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==============================Original Headers============================== 20, 14 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 1 10:04:20 2015 20, 14 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 20, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t01G4JjG016147 20, 14 -- for {microscopy-at-microscopy.com} ; Thu, 1 Jan 2015 10:04:20 -0600 20, 14 -- Message-ID: {54A57004.1030702-at-microscopy.com} 20, 14 -- Date: Thu, 01 Jan 2015 10:04:20 -0600 20, 14 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 14 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 14 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 20, 14 -- MIME-Version: 1.0 20, 14 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 14 -- Subject: Administrivia: Happy New Year 2015 20, 14 -- Content-Type: text/plain; charset=utf-8; format=flowed 20, 14 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Offline TIA viewer for windows.
Message: How to see .emi and .ser file on windows.? Is there any offline TIA viewer for windows available.?
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==============================Original Headers============================== 11, 17 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 2 18:33:07 2015 11, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t030X73P019812 11, 17 -- for {microscopy-at-microscopy.com} ; Fri, 2 Jan 2015 18:33:07 -0600 11, 17 -- Message-ID: {54A738C3.8090206-at-microscopy.com} 11, 17 -- Date: Fri, 02 Jan 2015 18:33:07 -0600 11, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 11, 17 -- MIME-Version: 1.0 11, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 17 -- Subject: viaWWW:Offline TIA viewer for windows 11, 17 -- References: {201501021738.t02HchTB029960-at-ns.microscopy.com} 11, 17 -- In-Reply-To: {201501021738.t02HchTB029960-at-ns.microscopy.com} 11, 17 -- X-Forwarded-Message-Id: {201501021738.t02HchTB029960-at-ns.microscopy.com} 11, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This won't help entirely, but ImageJ has a plugin which can open the .SER files. You need to be sure that you point to the correct file which contains the image. Most of the experimental information is stored in the .EMI file.
The FEI website has a link to an off-line version of ESVision (http://www.fei.com/service-support/es-vision/) which is the original version of TIA. You may be able to run it and analyze the data directly. (I've not tested it.)
Good luck, Henk
On 1/2/2015 7:35 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: ravi.thakkar369-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com } Name: Ravi } } Title-Subject: [Filtered] Offline TIA viewer for windows. } } Message: How to see .emi and .ser file on windows.? } Is there any offline TIA viewer for windows available.? } } Login Host: 129.130.146.69 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
When coming back from holidays, I found our CM10's monitor display was completely dark :( A small part of data display on a corner of the screen has been disappeared (fainted) for a while but the machine was operational without any problem. Now the whole screen was black out. The Data Dim and Panel Dim knobs were usually set at low or off during non-usage times. Other panel lights are all normal.
It seems like a small part (bulb or circuit board?) wearing out. Is there any simple way to check or order/replay the part?
Your expertise on this is much appreciated. Thanks you in advance.
Guosheng Liu University of Saskatchewan Canada
==============================Original Headers============================== 6, 36 -- From guosheng.liu-at-usask.ca Mon Jan 5 14:39:36 2015 6, 36 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t05Kda7Y018234 6, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 14:39:36 -0600 6, 36 -- Received: from conversion-daemon.usask.ca by usask.ca 6, 36 -- (Oracle Communications Messaging Server 7.0.5.34.0 64bit (built Oct 14 2014)) 6, 36 -- id {0NHQ007001CC0Q00-at-usask.ca} for Microscopy-at-microscopy.com; Mon, 6, 36 -- 05 Jan 2015 14:39:23 -0600 (CST) 6, 36 -- Received: from CAMPUSCAS4.usask.ca (campuscas4.usask.ca [128.233.194.195]) 6, 36 -- by usask.ca 6, 36 -- (Oracle Communications Messaging Server 7.0.5.34.0 64bit (built Oct 14 2014)) 6, 36 -- with ESMTPS id {0NHQ0042E1DNL560-at-usask.ca} for Microscopy-at-microscopy.com; Mon, 6, 36 -- 05 Jan 2015 14:39:23 -0600 (CST) 6, 36 -- Received: from CAMPUSMB4.usask.ca ([fe80::f90b:9124:ecc1:45ed]) 6, 36 -- by CAMPUSCAS4.usask.ca ([::1]) with mapi id 14.03.0195.001; Mon, 6, 36 -- 5 Jan 2015 14:39:22 -0600 6, 36 -- Date: Mon, 05 Jan 2015 20:39:21 +0000 6, 36 -- From: "Liu, Guosheng" {guosheng.liu-at-usask.ca} 6, 36 -- Subject: The data screen/monitor turns black in our Philips CM10 TEM---need 6, 36 -- help. 6, 36 -- X-Originating-IP: [10.65.80.9] 6, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 36 -- Message-id: {569262A9A49BF84695B7BFAE3D8E2A201795B657-at-CAMPUSMB4.usask.ca} 6, 36 -- MIME-version: 1.0 6, 36 -- Content-type: text/plain; charset=us-ascii 6, 36 -- Content-language: en-US 6, 36 -- Accept-Language: en-US 6, 36 -- Thread-topic: The data screen/monitor turns black in our Philips CM10 6, 36 -- TEM---need help. 6, 36 -- Thread-index: AdApJ687OIaT+yDFR1KAXCrWuAYUFQ== 6, 36 -- X-MS-Has-Attach: 6, 36 -- X-MS-TNEF-Correlator: 6, 36 -- x-pmwin-version: 3.1.2.0, Antivirus-Engine: 3.53.0, Antivirus-Data: 5.09 6, 36 -- x-puremessage: [Scanned] 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id t05Kda7Y018234 ==============================End of - Headers==============================
One thing you can try is to shine a bright light up in the upper left corner of the Data Monitor and see if the characters show up. The Data monitor intensity circuitry is a little strange. When you adjust the Data Dim knob it controls the intensity of a bulb on a circuit board behind the panel. There is a light absorbing diode that is next to the bulb which measures the intensity and adjust the circuitry that controls the Data brightness. If this bulb is burned out, then the monitor goes black.
Good Luck, John
-----Original Message----- X-from: guosheng.liu-at-usask.ca [mailto:guosheng.liu-at-usask.ca] Sent: Monday, January 05, 2015 3:58 PM To: John J. Schreiber
Happy new year EM community!
When coming back from holidays, I found our CM10's monitor display was completely dark :( A small part of data display on a corner of the screen has been disappeared (fainted) for a while but the machine was operational without any problem. Now the whole screen was black out. The Data Dim and Panel Dim knobs were usually set at low or off during non-usage times. Other panel lights are all normal.
It seems like a small part (bulb or circuit board?) wearing out. Is there any simple way to check or order/replay the part?
Your expertise on this is much appreciated. Thanks you in advance.
Guosheng Liu University of Saskatchewan Canada
==============================Original Headers============================== 6, 36 -- From guosheng.liu-at-usask.ca Mon Jan 5 14:39:36 2015 6, 36 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t05Kda7Y018234 6, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 14:39:36 -0600 6, 36 -- Received: from conversion-daemon.usask.ca by usask.ca 6, 36 -- (Oracle Communications Messaging Server 7.0.5.34.0 64bit (built Oct 14 2014)) 6, 36 -- id {0NHQ007001CC0Q00-at-usask.ca} for Microscopy-at-microscopy.com; Mon, 6, 36 -- 05 Jan 2015 14:39:23 -0600 (CST) 6, 36 -- Received: from CAMPUSCAS4.usask.ca (campuscas4.usask.ca [128.233.194.195]) 6, 36 -- by usask.ca 6, 36 -- (Oracle Communications Messaging Server 7.0.5.34.0 64bit (built Oct 14 2014)) 6, 36 -- with ESMTPS id {0NHQ0042E1DNL560-at-usask.ca} for Microscopy-at-microscopy.com; Mon, 6, 36 -- 05 Jan 2015 14:39:23 -0600 (CST) 6, 36 -- Received: from CAMPUSMB4.usask.ca ([fe80::f90b:9124:ecc1:45ed]) 6, 36 -- by CAMPUSCAS4.usask.ca ([::1]) with mapi id 14.03.0195.001; Mon, 6, 36 -- 5 Jan 2015 14:39:22 -0600 6, 36 -- Date: Mon, 05 Jan 2015 20:39:21 +0000 6, 36 -- From: "Liu, Guosheng" {guosheng.liu-at-usask.ca} 6, 36 -- Subject: The data screen/monitor turns black in our Philips CM10 TEM---need 6, 36 -- help. 6, 36 -- X-Originating-IP: [10.65.80.9] 6, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 36 -- Message-id: {569262A9A49BF84695B7BFAE3D8E2A201795B657-at-CAMPUSMB4.usask.ca} 6, 36 -- MIME-version: 1.0 6, 36 -- Content-type: text/plain; charset=us-ascii 6, 36 -- Content-language: en-US 6, 36 -- Accept-Language: en-US 6, 36 -- Thread-topic: The data screen/monitor turns black in our Philips CM10 6, 36 -- TEM---need help. 6, 36 -- Thread-index: AdApJ687OIaT+yDFR1KAXCrWuAYUFQ== 6, 36 -- X-MS-Has-Attach: 6, 36 -- X-MS-TNEF-Correlator: 6, 36 -- x-pmwin-version: 3.1.2.0, Antivirus-Engine: 3.53.0, Antivirus-Data: 5.09 6, 36 -- x-puremessage: [Scanned] 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id t05Kda7Y018234 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 41 -- From js51-at-princeton.edu Mon Jan 5 15:13:00 2015 17, 41 -- Received: from Princeton.EDU (ppa03.Princeton.EDU [128.112.128.214]) 17, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t05LCxv5007434 17, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 15:13:00 -0600 17, 41 -- Received: from csgsmtp201l.Princeton.EDU (csgsmtp201l.Princeton.EDU [128.112.134.60]) 17, 41 -- by ppa03.princeton.edu (8.14.5/8.14.5) with ESMTP id t05LCwte008008 17, 41 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 17, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 16:12:59 -0500 17, 41 -- Received: from CSGHUB209W.pu.win.princeton.edu (csghub209w.Princeton.EDU [128.112.128.68]) 17, 41 -- by csgsmtp201l.Princeton.EDU (8.13.8/8.12.9) with ESMTP id t05LCw95028402; 17, 41 -- Mon, 5 Jan 2015 16:12:58 -0500 17, 41 -- Received: from CSGMBX204W.pu.win.princeton.edu ([169.254.5.107]) by 17, 41 -- CSGHUB209W.pu.win.princeton.edu ([128.112.128.68]) with mapi id 17, 41 -- 14.03.0174.001; Mon, 5 Jan 2015 16:12:58 -0500 17, 41 -- From: "John J. Schreiber" {js51-at-princeton.edu} 17, 41 -- To: "guosheng.liu-at-usask.ca" {guosheng.liu-at-usask.ca} 17, 41 -- CC: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 17, 41 -- Subject: RE: [Microscopy] The data screen/monitor turns black in our Philips 17, 41 -- CM10 TEM---need 17, 41 -- Thread-Topic: [Microscopy] The data screen/monitor turns black in our 17, 41 -- Philips CM10 TEM---need 17, 41 -- Thread-Index: AQHQKSpaN5c4gssm1kytWb/5J777E5yyBEEg 17, 41 -- Date: Mon, 5 Jan 2015 21:12:57 +0000 17, 41 -- Message-ID: {3C628C43B600C14EB700249D43D718D30E7497B9-at-CSGMBX204W.pu.win.princeton.edu} 17, 41 -- References: {201501052058.t05KwQuc005842-at-ns.microscopy.com} 17, 41 -- In-Reply-To: {201501052058.t05KwQuc005842-at-ns.microscopy.com} 17, 41 -- Accept-Language: en-US 17, 41 -- Content-Language: en-US 17, 41 -- X-MS-Has-Attach: 17, 41 -- X-MS-TNEF-Correlator: 17, 41 -- x-originating-ip: [128.112.141.39] 17, 41 -- Content-Type: text/plain; charset="us-ascii" 17, 41 -- MIME-Version: 1.0 17, 41 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.13.68,1.0.33,0.0.0000 17, 41 -- definitions=2015-01-05_04:2015-01-05,2015-01-05,1970-01-01 signatures=0 17, 41 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 17, 41 -- suspectscore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 17, 41 -- adjust=0 reason=mlx scancount=1 engine=7.0.1-1402240000 17, 41 -- definitions=main-1501050208 17, 41 -- Content-Transfer-Encoding: 8bit 17, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t05LCxv5007434 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both roy.geiss-at-colostate.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: roy.geiss-at-colostate.edu Name: Roy H. Geiss
Organization: Colorado State University
Title-Subject: [Filtered] Summer School on Electron Diffraction
Message: We are happy to announce the second edition of the CIF Summer School, which this year will feature a 3-day workshop on electron diffraction methods for materials analysis (May 19-21, 2015). Registration will open mid-January to both CSU and non-CSU students and researchers. For more information, go to: http://cif.colostate.edu/cif-summer-school/.
Thank you. Regards, Karolien
Karolien Denef Associate Director/Research Scientist Central Instrument Facility (CIF) Department of Chemistry - Colorado State University Fort Collins, CO 80523-1872 970-491- 3832 (o); 970-556-4846 (cell) http://cif.colostate.edu/
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==============================Original Headers============================== 17, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 5 20:04:40 2015 17, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 17, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0624eGX004992 17, 17 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 20:04:40 -0600 17, 17 -- Message-ID: {54AB42B8.30808-at-microscopy.com} 17, 17 -- Date: Mon, 05 Jan 2015 20:04:40 -0600 17, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 17, 17 -- MIME-Version: 1.0 17, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 17 -- Subject: viaWWW:CSU Summer School on Electron Diffraction 17, 17 -- References: {201501051905.t05J5twj015449-at-ns.microscopy.com} 17, 17 -- In-Reply-To: {201501051905.t05J5twj015449-at-ns.microscopy.com} 17, 17 -- X-Forwarded-Message-Id: {201501051905.t05J5twj015449-at-ns.microscopy.com} 17, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jennifer.korrell-at-nih.gov Name: Jennifer Korrell
Message: Leidos Biomedical Research Inc. is seeking a dedicated, driven Research Associate II to join their Electron Microscopy Laboratory. This position is located in Frederick, MD.
Below is a link that will take you directly to the job description on our website. If interested, please apply using the "apply" button at the bottom of the page.
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==============================Original Headers============================== 16, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 5 20:05:27 2015 16, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0625Rlo005829 16, 17 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 20:05:27 -0600 16, 17 -- Message-ID: {54AB42E7.4080805-at-microscopy.com} 16, 17 -- Date: Mon, 05 Jan 2015 20:05:27 -0600 16, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 16, 17 -- MIME-Version: 1.0 16, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 17 -- Subject: viaWWW:Job Opportunity- Leidos Biomed 16, 17 -- References: {201501052108.t05L8Zbv007387-at-ns.microscopy.com} 16, 17 -- In-Reply-To: {201501052108.t05L8Zbv007387-at-ns.microscopy.com} 16, 17 -- X-Forwarded-Message-Id: {201501052108.t05L8Zbv007387-at-ns.microscopy.com} 16, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am in the early stages of my career as a facility director and was wondering if you could lend some tips for negotiating service contract prices.
Our facility has a TEM and SEM, both of which are under service contracts with FEI. The increase in price has been pretty steady over the years, i.e. ~$200 for the SEM and ~$500 for the TEM. This year, however, the increase in price for the SEM contract is ~$1,300!! This was even after the mutli-contract discount.
I did not expect such an increase this year so my budget was not adjusted accordingly. I would say that I was pretty unhappy with the SEM service this past year. Despite contacting my field service engineer multiple times, I never heard back or it took over a month to hear back. We are also still having low vac problems that have resulted in countless hours of down time. Should I mention these points to my sales rep?
I'm new to the whole business side of science and I am wondering if anyone has any suggestions. I wouldn't want to say something wrong.....
Thank you in advance!
-Blanca
==============================Original Headers============================== 7, 28 -- From bicarbaj-at-mtholyoke.edu Mon Jan 5 21:02:20 2015 7, 28 -- Received: from mail-qa0-f43.google.com (mail-qa0-f43.google.com [209.85.216.43]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0632K9S016234 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 21:02:20 -0600 7, 28 -- Received: by mail-qa0-f43.google.com with SMTP id n4so12813048qaq.30 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 05 Jan 2015 19:02:19 -0800 (PST) 7, 28 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 28 -- d=1e100.net; s=20130820; 7, 28 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to 7, 28 -- :content-type; 7, 28 -- bh=+gOabxjl/kKejBr9QtA8GHwB8BxOpFPF1/e5j2kL9ug=; 7, 28 -- b=Amkbww+76aocR+RPffhMCeCoHRQ+QhDbSYD2qLBMCZ6sGmrefz4FlNzWM/cdVYHCsy 7, 28 -- S8mupsgCRTxN86MhrXyBjpQ6NaSlqhvoUBxNxo1c/I3nOleS8Pk5Dt0qrZCNY+fIQOcV 7, 28 -- kP+Y73GsFNMyWJlb064vsNm4X9R+HKp2GWocQyPgBRs1tyEY1kQgc3xHSB46qXfbWPdk 7, 28 -- tLuEu3wOcTWBh5dowZO9QmF2cJXAyHvz7LYWrBP5OpVf4i7fthSh7lxmJdO9y9IklgpL 7, 28 -- obgCmL3hxyAbqan/x//k8I3BkUmi0ABfAs9dIU2s4VT9aXQSao9y2KfV+gIY54zgYUTF 7, 28 -- mLnA== 7, 28 -- X-Gm-Message-State: ALoCoQmYZLxpHyfvhreduK0PvHAFDvvWolUnN1G8IAMT2d++xBtEYy2GyBeI95LgGgCRdacNnoQ4 7, 28 -- MIME-Version: 1.0 7, 28 -- X-Received: by 10.140.108.9 with SMTP id i9mr59518512qgf.73.1420513339566; 7, 28 -- Mon, 05 Jan 2015 19:02:19 -0800 (PST) 7, 28 -- Received: by 10.96.118.1 with HTTP; Mon, 5 Jan 2015 19:02:19 -0800 (PST) 7, 28 -- Date: Mon, 5 Jan 2015 22:02:19 -0500 7, 28 -- Message-ID: {CAO94suncLfadFc2=b_TYFnr4Z+SZN0szUpB-Kqsr3AZKg_cpwA-at-mail.gmail.com} 7, 28 -- Subject: EM service contracts-Negotiating prices? 7, 28 -- From: Blanca Carbajal Gonzalez {bicarbaj-at-mtholyoke.edu} 7, 28 -- To: microscopy-at-microscopy.com 7, 28 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
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} realname - Ondrej Kotecky } Email - ondrej.kotecky-at-gmail.com } EDUCATION - Graduate College } LOCATION - Brno, Czech Republic } QUESTION - Hello, } } i had rubber sample made from two rubber components/compounds. The interface between the components was not visible in reflected light on a guillotine cut, and it was not visible on a polished section. (Polished similarly as a metallurgical sample. Polishing resulted in a nearly plane and slightly inclined surface.) Neither dark-field nor polarized-light could resolve the interface -- one colour across the whole sample. } } } From the above, i was assuming that there is no step in between the components (dark-field) and that the compounds give the same reflection in polarized light. So DIC should not show the interface. I tried anyway and was surprised to see two different colours on both sides of the interface. } } I'd like to understand why DIC shows a different colour. The literature did not help. I must be missing something. Could you help to explain why DIC results in two homogeneous regions with a distinct colour contrast? } } Many thanks, } Ondrej Kotecky
==============================Original Headers============================== 4, 33 -- From oshel1pe-at-cmich.edu Wed Jan 7 07:07:14 2015 4, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07D7CVf029518 4, 33 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 07:07:13 -0600 4, 33 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 4, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t07D79Sn019994 4, 33 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 08:07:10 -0500 4, 33 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 4, 33 -- cas1.central.cmich.local (2002:8dd1:f2b::8dd1:f2b) with Microsoft SMTP Server 4, 33 -- (TLS) id 14.3.195.1; Wed, 7 Jan 2015 08:07:09 -0500 4, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 33 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 4, 33 -- 14.3.195.1; Wed, 7 Jan 2015 08:07:08 -0500 4, 33 -- Message-ID: {54AD2F73.1080701-at-cmich.edu} 4, 33 -- Date: Wed, 7 Jan 2015 08:06:59 -0500 4, 33 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 4, 33 -- Reply-To: Ondrej Kotecky {ondrej.kotecky-at-gmail.com} 4, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 33 -- MIME-Version: 1.0 4, 33 -- To: micro {microscopy-at-microscopy.com} 4, 33 -- Subject: Re: Ask-A-Microscopist: DIC light microscopy of 2 rubber compounds 4, 33 -- in contact 4, 33 -- References: {1983698105.1257.1420620947588.JavaMail.EWHSERVER1324$-at-10.10.133.40} 4, 33 -- In-Reply-To: {1983698105.1257.1420620947588.JavaMail.EWHSERVER1324$-at-10.10.133.40} 4, 33 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 4, 33 -- Content-Transfer-Encoding: 7bit 4, 33 -- X-Originating-IP: [141.209.2.100] 4, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 33 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 33 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 4, 33 -- X-CanItPRO-Stream: default 4, 33 -- X-Canit-Stats-ID: 02NB17alG - ef731ed09bda - 20150107 4, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Email: guy.vermeildeconchard-at-fr.netgrs.com Name: Guy VERMEIL DE CONCHARD
Organization: BIOLOGIE SERVIER COMPANY
Title-Subject: [Filtered] genuine spare part JEM 100SX
Message: To be given 8 never used filaments type K (tungsten) Jeol part No. 804500070 - type MA113008(03) for Jeol transmission microscope type 100SX.
please, contact by mail.
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 7 07:55:44 2015 14, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07Dtibi017872 14, 17 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 07:55:44 -0600 14, 17 -- Message-ID: {54AD3AE0.2050205-at-microscopy.com} 14, 17 -- Date: Wed, 07 Jan 2015 07:55:44 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW: never used filaments genuine spare part JEM 100SX 14, 17 -- References: {201501070832.t078W4ln022210-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201501070832.t078W4ln022210-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201501070832.t078W4ln022210-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Organization: University of Massachusetts Medical School
Title-Subject: [Filtered] Recycling old EM negatives
Message: A colleague of mine found several boxes of old (from her dissertation) EM negatives while cleaning out her basement. She contacted me and asked if we could recycle them. It never crossed my mind that these could be recycled but apparently there are hundreds of them and it does seem kind of a waste to just through them out. Does anyone out there no of/or if Old EM negatives (Kodak 4489) can be recycled? Happy New year to you all Greg
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==============================Original Headers============================== 13, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 7 07:56:32 2015 13, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07DuWV8018798 13, 17 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 07:56:32 -0600 13, 17 -- Message-ID: {54AD3B10.3040609-at-microscopy.com} 13, 17 -- Date: Wed, 07 Jan 2015 07:56:32 -0600 13, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 13, 17 -- MIME-Version: 1.0 13, 17 -- To: microscopyListServer-Forward {microscopy-at-microscopy.com} 13, 17 -- Subject: viaWWW:Recycling old EM negatives 13, 17 -- References: {201501071333.t07DXCTi017215-at-ns.microscopy.com} 13, 17 -- In-Reply-To: {201501071333.t07DXCTi017215-at-ns.microscopy.com} 13, 17 -- X-Forwarded-Message-Id: {201501071333.t07DXCTi017215-at-ns.microscopy.com} 13, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
X-from a quick google, Kodak have a document called KES-60, which has a list of scrap film buyers, the most recent version I could find in Google was this:
On 07/01/2015 14:04, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
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==============================Original Headers============================== 10, 30 -- From ben.micklem-at-pharm.ox.ac.uk Wed Jan 7 08:13:02 2015 10, 30 -- Received: from relay15.mail.ox.ac.uk (relay15.mail.ox.ac.uk [163.1.2.163]) 10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07ED1jB024855 10, 30 -- for {Microscopy-at-Microscopy.com} ; Wed, 7 Jan 2015 08:13:02 -0600 10, 30 -- Received: from hub01.nexus.ox.ac.uk ([163.1.154.218] helo=HUB01.ad.oak.ox.ac.uk) 10, 30 -- by relay15.mail.ox.ac.uk with esmtp (Exim 4.80) 10, 30 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 10, 30 -- id 1Y8rM5-0005ye-n2 10, 30 -- for Microscopy-at-Microscopy.com; Wed, 07 Jan 2015 14:13:01 +0000 10, 30 -- Received: from MBX03.ad.oak.ox.ac.uk ([169.254.3.5]) by HUB01.ad.oak.ox.ac.uk 10, 30 -- ([163.1.154.92]) with mapi id 14.03.0169.001; Wed, 7 Jan 2015 14:13:00 +0000 10, 30 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 10, 30 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 10, 30 -- Subject: Re: [Microscopy] viaWWW:Recycling old EM negatives 10, 30 -- Thread-Topic: [Microscopy] viaWWW:Recycling old EM negatives 10, 30 -- Thread-Index: AQHQKoLPirOrbQ6QOkSUnvankMEcHpy0sw0A 10, 30 -- Date: Wed, 7 Jan 2015 14:13:00 +0000 10, 30 -- Message-ID: {D0D2EEF2.3F3B9%ben.micklem-at-pharm.ox.ac.uk} 10, 30 -- In-Reply-To: {201501071404.t07E48kb004689-at-ns.microscopy.com} 10, 30 -- Accept-Language: en-GB, en-US 10, 30 -- Content-Language: en-US 10, 30 -- X-MS-Has-Attach: 10, 30 -- X-MS-TNEF-Correlator: 10, 30 -- user-agent: Microsoft-MacOutlook/14.3.4.130416 10, 30 -- x-originating-ip: [172.16.150.240] 10, 30 -- Content-Type: text/plain; charset="utf-8" 10, 30 -- Content-ID: {85A27FDF5D9B4C4F95242DE7ED67ECE0-at-ad.oak.ox.ac.uk} 10, 30 -- MIME-Version: 1.0 10, 30 -- Content-Transfer-Encoding: 8bit 10, 30 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t07ED1jB024855 ==============================End of - Headers==============================
Thank you very much for all the helpful advice and kind words. I learned about many alternatives for service that I will definitely be considering in the future.
I found out what the issue was with FEI. Apparently, we were being given an unusual 5% multi-tool discount which was reduced to 2% this year. However, we were not given notice of this reduction last year when we were preparing our budget (this happened before I started the position).
FEI was kind enough to negotiate to a 3.5% discount for this year, mostly because our budget was prepared with a 5% discount in mind.
More good news: I contacted our service engineer about the continued low vac problem. His response this time was prompt and he is coming out to inspect the instrument at the end of the week.
Before my post, I was not aware that the best plan of action was to voice my concerns to the service manager when I found myself dissatisfied with the service. Thank you for letting me know. Maybe now I won't have issues with obtaining prompt replies.
Thank you,
Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu
==============================Original Headers============================== 9, 28 -- From bicarbaj-at-mtholyoke.edu Wed Jan 7 11:45:33 2015 9, 28 -- Received: from mail-qg0-f50.google.com (mail-qg0-f50.google.com [209.85.192.50]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07HjXq2018025 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 11:45:33 -0600 9, 28 -- Received: by mail-qg0-f50.google.com with SMTP id z60so1176036qgd.37 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 07 Jan 2015 09:45:33 -0800 (PST) 9, 28 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 28 -- d=1e100.net; s=20130820; 9, 28 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to 9, 28 -- :content-type; 9, 28 -- bh=oiZ7Ele5Gm/qgS0h/zXTxDHcHhxlYYc3rghDW9Udld8=; 9, 28 -- b=QyP8hzqzOXbut6MyFu1e1gOO6SRnY0XB14KpwW4/dVU0OsbAPArbnJYVc9jx63Ql7K 9, 28 -- /nPU200c3t9jF9tViE6GhgrtQD75O00iHeZVApmmITMQPhIUqrtGjDo4c5qM7nNcv4EA 9, 28 -- 9VzX1+gp78VC1wzNAA+LXysIDxhINxA3D1N7W884CZf/Mnev/xqwBRIfnKWewLkyUPHm 9, 28 -- qrxfXr2gI7pdnPwq2yowMWaxqeJbFF7aWxrxjz1bxVXMBLJM+gz00EGcEiljyIh+bftT 9, 28 -- OQ+glOpFZxTFcDqvopihuvmwOUwzJf1oP0UZ3kR9UlxfiUB9/Z5CoDWJ8S8GMAgA23rK 9, 28 -- ak4g== 9, 28 -- X-Gm-Message-State: ALoCoQl8Qd6Jzkw6C0+QKU4RKXstbvk6ts2sRG7dZRtTtUaQ/UWhWYFp5R3rPRnqEsSen9PZ9onb 9, 28 -- MIME-Version: 1.0 9, 28 -- X-Received: by 10.224.43.3 with SMTP id u3mr6832680qae.50.1420652732929; Wed, 9, 28 -- 07 Jan 2015 09:45:32 -0800 (PST) 9, 28 -- Received: by 10.96.118.1 with HTTP; Wed, 7 Jan 2015 09:45:32 -0800 (PST) 9, 28 -- Date: Wed, 7 Jan 2015 12:45:32 -0500 9, 28 -- Message-ID: {CAO94su=N5J2iC_wocctoCpo2UCxY5ZPmBV=0JfoN+Fe7Pcaiig-at-mail.gmail.com} 9, 28 -- Subject: EM service contracts-Negotiating prices?--update 9, 28 -- From: Blanca Carbajal Gonzalez {bicarbaj-at-mtholyoke.edu} 9, 28 -- To: microscopy-at-microscopy.com 9, 28 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
There are people around the world that recovers silver from both unexposed film and negatives. Many have gathered together on the http://goldrefiningforum.com/ forum. There you could probably find someone close-by that can recover the silver from the films. The process is also described on several places on the forum for anyone interested.
/Göran
ben.micklem-at-pharm.ox.ac.uk skrev den 2015-01-07 15:13: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from a quick google, Kodak have a document called KES-60, which has a list } of scrap film buyers, the most recent version I could find in Google was } this: } } http://www.kodak.co.uk/ek/uploadedFiles/Content/About_Kodak/Global_Sustaina } bility/Health,_Safety_and_Environment/HSE_Support_Center/Product_End_of_Lif } e_Management/KES-60_Scrap_Film_Buyers.pdf } } } Ben } } } } On 07/01/2015 14:04, "microscopylistserver-noreply-at-microscopy.com" } {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } } } } -------------------------------------------------------------------------- } } -- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------------------- } } -- } } } } X-from: Gregory.Hendricks-at-umassmed.edu } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } -------------------------------------------------------------------------- } } - } } Remember this posting is most likely not from a Subscriber, so when } } replying } } please copy both Gregory.Hendricks-at-umassmed.edu as well as the } } Microscopy Listserver } } -------------------------------------------------------------------------- } } - } } } } Email: Gregory.Hendricks-at-umassmed.edu } } Name: Greg Hendricks } } } } Organization: University of Massachusetts Medical School } } } } Title-Subject: [Filtered] Recycling old EM negatives } } } } Message: A colleague of mine found several boxes of old (from her } } dissertation) EM negatives while } } cleaning out her basement. She contacted me and asked if we could } } recycle them. It never crossed } } my mind that these could be recycled but apparently there are hundreds of } } them and it does seem kind } } of a waste to just through them out. Does anyone out there no of/or if } } Old EM negatives (Kodak } } 4489) can be recycled? } } Happy New year to you all } } Greg } } } } Login Host: 146.189.245.29 } } Listserver Email Form V - 20120416 } } -------------------------------------------------------------------------- } } - } } } } } } } } } } -- } } =========================================== } } Do not reply to this message it is from } } the Microscopy Listserver NO-REPLY forwarding } } system. 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==============================Original Headers============================== 4, 24 -- From axelsson-at-acc.umu.se Wed Jan 7 17:34:03 2015 4, 24 -- Received: from mail.acc.umu.se (mail.acc.umu.se [130.239.18.156]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07NY2JX016771 4, 24 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 17:34:03 -0600 4, 24 -- Received: from localhost (localhost [127.0.0.1]) 4, 24 -- by amavisd-new (Postfix) with ESMTP id 909EBB40 4, 24 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 00:34:02 +0100 (MET) 4, 24 -- X-Virus-Scanned: amavisd-new at acc.umu.se 4, 24 -- Received: from [192.168.1.140] (dynamic.1.23.c4acbfe70.0405c18da6.cust.bredband2.com [89.233.195.134]) 4, 24 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 4, 24 -- (No client certificate requested) 4, 24 -- by mail.acc.umu.se (Postfix) with ESMTPS id E67B2B3F 4, 24 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 00:34:00 +0100 (MET) 4, 24 -- Message-ID: {54ADC263.3050900-at-acc.umu.se} 4, 24 -- Date: Thu, 08 Jan 2015 00:33:55 +0100 4, 24 -- From: =?windows-1252?Q?G=F6ran_Axelsson?= {axelsson-at-acc.umu.se} 4, 24 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 4, 24 -- MIME-Version: 1.0 4, 24 -- To: microscopy-at-microscopy.com 4, 24 -- Subject: Re: [Microscopy] Re: viaWWW:Recycling old EM negatives 4, 24 -- References: {201501071413.t07ED73e024909-at-ns.microscopy.com} 4, 24 -- In-Reply-To: {201501071413.t07ED73e024909-at-ns.microscopy.com} 4, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 24 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: leex3870-at-umn.edu Name: Han
Organization: University of Minnesota
Title-Subject: [Filtered] Compresser problem in RMC RFD-9010CR freeze fracture machine
Message: Hello
I have a RMC RFD-9010CR freeze fracture machine and its compressor (Mitsubishi super line SP-KR) is not working. I checked fuses in the mainboard, and none of them is blown. I'm wondering that anyone had similar problem with RMC freeze fracture machine can give some advice so that I can start with. Thanks, Han
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==============================Original Headers============================== 12, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 8 06:54:37 2015 12, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t08Csbfd013530 12, 17 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 06:54:37 -0600 12, 17 -- Message-ID: {54AE7E0D.5010402-at-microscopy.com} 12, 17 -- Date: Thu, 08 Jan 2015 06:54:37 -0600 12, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 12, 17 -- MIME-Version: 1.0 12, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 17 -- Subject: viaWWW:Compresser problem in RMC RFD-9010CR freeze fracture machine 12, 17 -- References: {201501072320.t07NKinB016338-at-ns.microscopy.com} 12, 17 -- In-Reply-To: {201501072320.t07NKinB016338-at-ns.microscopy.com} 12, 17 -- X-Forwarded-Message-Id: {201501072320.t07NKinB016338-at-ns.microscopy.com} 12, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: afmhelp-at-gmail.com Name: Peter Eaton
Organization: UCIBIO-at-Requimte / University of Porto, Portugal
Title-Subject: [Filtered] AFM Training course 2015
Message: Dear All, The 4th Porto Atomic Force Microscopy Training Workshop will run during Easter 2015, from the 30th March to 2nd April in the historic city of Porto, Portugal. Following the successful courses that ran in 2011, 2013 and 2014, the course will include several hours hands-on training in acquiring images with the atomic force microscope as well as AFM data processing. Other topics covered in lectures include AFM modes, AFM instrumentation, sample preparation, and applications. The course will also feature advanced topics lectures from guest scientists in biology and materials science. Those interested in attending are encouraged to register as soon as possible, as the course usually fills quickly. More details can be found at http://afmhelp.com/course, and enquiries can be sent to afmhelp-at-gmail.com. Regards, Dr. Peter Eaton
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 8 06:55:39 2015 14, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t08CtdEW014194 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 06:55:39 -0600 14, 17 -- Message-ID: {54AE7E4B.4020500-at-microscopy.com} 14, 17 -- Date: Thu, 08 Jan 2015 06:55:39 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:AFM Training course 2015 - Portugal 14, 17 -- References: {201501081019.t08AJ3Mf009403-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201501081019.t08AJ3Mf009403-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201501081019.t08AJ3Mf009403-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You may recall that I asked the list about what to include in a bachelor's level EM curriculum.
I received many responses, mostly directly to me, and a few that were shared on the list.
I am still working on this project and want to thank everyone who has contributed. Keep those cards and letters coming, more ideas are always better.
I got several requests to post the replies to the list, but the page count is now up to 11 and I think that's too long to post. So, if you would like to receive a copy of the replies, send me a request and I will send a PDF to you. Fear not, I think I have sanitized the responses so you have nothing to fear from the thought police. If you sent a reply and do not wish to have it shared, let me know and I will take care of it.
Jon
-- Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Avenue Stockton, CA 95207 jkrupp-at-deltacollege.edu (209) 954-5284
Find us on Facebook at Electron Microscopy at SJ Delta College
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Email: czyrska-at-agh.edu.pl Name: Aleksandra Czyrska-Filemonowicz
Organization: Centre of Electron Microscopy for Materials Science, AGH University of Science and Technology, Krakow, Poland
Title-Subject: [Filtered] Post-doctorate or senior electron microscopist
Message: The Faculty of Metals Engineering and Industrial Computer Sciences and International Centre of Electron Microscopy for Materials Science (IC-EM) invite applications for an experienced Post-Doctorate Scientist in materials science and/or physics to strengthen and further develop an eleven people team.
The present research frame is focused on quantitative characterisation of micro/nanostructure and properties of various materials, structure-property relationship as well as tailoring microstructure for desired properties. Transmission electron microscopy (TEM) is the main investigation tool around a probe Cs corrected Titan Cubed 60-300 with a ChemiSTEM, Merlin Gemini II SEM and comprehensive preparation laboratory with FIB and NanoMill 1040. More details are given on the Centre website: http://www.tem.agh.edu.pl
This offer aims at a person with a PhD degree in materials science or physics. Proven ability to conduct academic research (mainly using TEM), ability to make clear oral and written presentations as well as self-motivation are prerequisite. Ability to write publications in international journals, fluent English (spoken and written) are expected. Teaching ability in English and in Polish is required. Female scientists are encouraged to apply.
The successful candidate will take the responsibility for research within existing projects and is expected to develop her/his own projects on a longer perspective based on national, international and industry funding.
The call is open immediately and will be closed on 10th of February, 2015. The University position will be open from 1st of March 2015 and secure for the next two years with a possibility of further prolongation.
Please send your application (CV and publications list) together with the names of two referees (phone, e-mail and website addresses) by e-mail to:
Prof. Dr. Aleksandra Czyrska-Filemonowicz AGH University of Science and Technology, Krakow, Poland Tel. +48 12 617 2929 or +48 12 617 2587 (secretary) email: czyrska-at-agh.edu.pl http://www.tem.agh.edu.pl
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==============================Original Headers============================== 25, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 8 18:19:10 2015 25, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 25, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t090JAsS002956 25, 17 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 18:19:10 -0600 25, 17 -- Message-ID: {54AF1E7E.8080701-at-microscopy.com} 25, 17 -- Date: Thu, 08 Jan 2015 18:19:10 -0600 25, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 25, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 25, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 25, 17 -- MIME-Version: 1.0 25, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 25, 17 -- Subject: viaWWW:Post-doctorate or senior electron microscopist / Krakow, Poland 25, 17 -- References: {201501081527.t08FRFu3027322-at-ns.microscopy.com} 25, 17 -- In-Reply-To: {201501081527.t08FRFu3027322-at-ns.microscopy.com} 25, 17 -- X-Forwarded-Message-Id: {201501081527.t08FRFu3027322-at-ns.microscopy.com} 25, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 25, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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I'm looking for input on preparing some very testy powder samples of mesoporous silica embedded with small metal nanoparticles. The goal is to get cross-sectional HRTEM to show that the particles are embedded into the walls of the silica, instead in the pores/surface. Imaging the material as powder doesn't show any of the tubular bundles in the correct orientation (looking down the pores), so I've tried cross sections.
FIB chewed up the material, so the next approach was to embed in epoxy and microtome slices. My collaborator used a general procedure from the technical guide, 19:21:1.2 Araldite502:DDSA:BDMA with powder mixed in, at 60C for 24h after degassing, in BEEM capsules. The epoxy slices (70-200nm) were relatively soft, had a couple bubbles, and often split lengthwise during cutting. The admin of our facility suggested that the polymerizarion was unfinished. Under our FEI Titan beam at both 80kV and 300kV, the slices crumpled & broke immediately at medium magnification (3800X) with a spread beam (spot size 4), and including after exposure/pre-warming at low mag, which was ok.
Assuming that microtoming is the way to go, can anyone suggest modifications to the embedding procedure to make the slices more robust? Or TEM tricks to prevent breaking? Or, what techniques might be able to cross section powder better? The silica pores/channels (~10nm diameter) also present a challenge because they swell under the beam, and being embedded makes the ~5nm nps harder to find...
Thanks to anyone who read all that! Those of us on this projects are new at the microtome, so all input is very appreciated.
-Chilan
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==============================Original Headers============================== 18, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 8 18:20:13 2015 18, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 18, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t090KDep003744 18, 17 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 18:20:13 -0600 18, 17 -- Message-ID: {54AF1EBD.4020802-at-microscopy.com} 18, 17 -- Date: Thu, 08 Jan 2015 18:20:13 -0600 18, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 18, 17 -- MIME-Version: 1.0 18, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 17 -- Subject: viaWWW:Cross-section Preparation of mesoporous silica 18, 17 -- References: {201501081858.t08IwhZo018915-at-ns.microscopy.com} 18, 17 -- In-Reply-To: {201501081858.t08IwhZo018915-at-ns.microscopy.com} 18, 17 -- X-Forwarded-Message-Id: {201501081858.t08IwhZo018915-at-ns.microscopy.com} 18, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
If you asked for a PDF of the replies I got to my question about EM curriculum, sit tight.
The file is on my other computer and I won't get to it for about a week. Will send out when I can.
Jon
-- Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Avenue Stockton, CA 95207 jkrupp-at-deltacollege.edu (209) 954-5284
Find us on Facebook at Electron Microscopy at SJ Delta College
I've looked at many specimens of mesoporous silica embedded with nanoparticles in my time, as well as a fair few microtomed specimens - and have a few suggestions;
1) Stop using TEM - use STEM instead, preferably some flavour of ADF imaging as the contrast from the metal nanoparticles will be strong, and there will be no phase contrast making interpretation harder.
Also both mesoporous silica and the embedding polymer undergo damage via electron beam heating and/or electrostatic charging induced tearing. As the total dose rate is much lower in STEM (and a moving probe means no area is exposed for long) damage will be much less of a problem. Also note this means high voltage means LESS damage for the silica - as the inelastic cross section drops with increasing voltage. As such 300kV is orders of magnitude more stable than 80kV for these materials.
2) Coat your sections with a monolayer of carbon after microtoming. As heating/charging is the damage mechanism this will provide a conduction path for removing charge and heat. (In some microtomed sections this converts specimens from being unusable in STEM to perfectly stable).
3) To make it easier to see end on pores in the powder you should grind the sample before dispersing it in alcohol - and sonicate it well. The raw material tends to form in micelles that are 'sausage' shaped with the pores along the long axis of the sausage. This is what makes it unlikely to come across end on pores in an unground specimen.
4) Tilt! If you cant see end on pores when flat your microscope has a double tilt stage - use it. Even better carry out tomography as you'll be able to resolve internal/external and particle positions in the reconstruction. Note that whatever your specimen prep technique only the tilting approaches will allow you to say with any certainty where your nanoparticles are in relation to the silica - all other imaging techniques will be limited by projection.
Hope that helps,
Matthew
On 9/01/2015 11:39 AM, microscopylistserver-noreply-at-microscopy.com wrote:
} Organization: UCLA Materials Science } } Title-Subject: [Filtered] Cross-section Preparation } } Message: Dear all, } } I'm looking for input on preparing some very testy powder samples of } mesoporous silica embedded with small metal nanoparticles. The goal } is to get cross-sectional HRTEM to show that the particles are } embedded into the walls of the silica, instead in the pores/surface. } Imaging the material as powder doesn't show any of the tubular } bundles in the correct orientation (looking down the pores), so I've } tried cross sections. } } FIB chewed up the material, so the next approach was to embed in } epoxy and microtome slices. My collaborator used a general procedure } from the technical guide, 19:21:1.2 Araldite502:DDSA:BDMA with powder } mixed in, at 60C for 24h after degassing, in BEEM capsules. The epoxy } slices (70-200nm) were relatively soft, had a couple bubbles, and } often split lengthwise during cutting. The admin of our facility } suggested that the polymerizarion was unfinished. Under our FEI Titan } beam at both 80kV and 300kV, the slices crumpled & broke immediately } at medium magnification (3800X) with a spread beam (spot size 4), and } including after exposure/pre-warming at low mag, which was ok. } } Assuming that microtoming is the way to go, can anyone suggest } modifications to the embedding procedure to make the slices more } robust? Or TEM tricks to prevent breaking? Or, what techniques might } be able to cross section powder better? The silica pores/channels } (~10nm diameter) also present a challenge because they swell under } the beam, and being embedded makes the ~5nm nps harder to find... } } Thanks to anyone who read all that! Those of us on this projects are } new at the microtome, so all input is very appreciated. } } -Chilan
-- Dr M.Weyland, Associate Professor & Titan Manager -------------------------------------------------------------------------- Monash Centre for Electron Microscopy Bldg 81 Monash University Victoria 3800 Australia. -------------------------------------------------------------------------- www.mcem.monash.edu.au ------------------------------------------------------------------------- Ph: (+61) 3 990 59026 --- Fax: (+61) 3 990 53600 --------------------------------------------------------------------------
==============================Original Headers============================== 13, 41 -- From matthew.weyland-at-monash.edu Thu Jan 8 23:03:06 2015 13, 41 -- Received: from na3sys009aog124.obsmtp.com (na3sys009aog124.obsmtp.com [74.125.149.151]) 13, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t09535V2008403 13, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 23:03:05 -0600 13, 41 -- Received: from mail-pd0-f172.google.com ([209.85.192.172]) (using TLSv1) by na3sys009aob124.postini.com ([74.125.148.12]) with SMTP 13, 41 -- ID DSNKVK9hCEGWqGRuEgORF/rrdAYXLncB/XOB-at-postini.com; Thu, 08 Jan 2015 21:03:06 PST 13, 41 -- Received: by mail-pd0-f172.google.com with SMTP id y13so15335057pdi.3 13, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 08 Jan 2015 21:03:04 -0800 (PST) 13, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 41 -- d=1e100.net; s=20130820; 13, 41 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 13, 41 -- :cc:subject:references:in-reply-to:content-type 13, 41 -- :content-transfer-encoding; 13, 41 -- bh=ebziDJocUcY6TywWTdDslsV8/iSZ83iZN3o2G4WEEZM=; 13, 41 -- b=Sc62iB/zdFK8uRooX72/Z16uH9leu5KWOToag/K9U2ZTp0rQmUbEpeAOAgV1hJkqk2 13, 41 -- MftBtGTLS77z1s/Bz0PHO5KYcIjgfw8MCokBZ75IYaD2UaBt5Kuuuw/T4c6u1cIzNIV1 13, 41 -- ifDHwFRSpTFfiZmc3A12rmlp+yoWrpEVYiyS2ofG4qZ5hwbLh9T4y/mSc21gOkO0SeZL 13, 41 -- 3ndvv+/X/KTA059tR8ne9pkWPqivycyka5XGlXTJoPPgEW1y+sm8+cTCMPIpUqZ7Hmp3 13, 41 -- 0zKW4ELk+W6Lap9c59g1YpjdWe/JxvE8XnLlxexFBW8vE62iNsfOoh5O5aVkJzt5A50g 13, 41 -- Py2Q== 13, 41 -- X-Gm-Message-State: ALoCoQlXneIBL7JEHomgcoortAz0GsvyVR32b5OA72EhF8P1S0UjnG/W95FBi6mLFwNVSsZbLkSnn6hAliJWAiGdW4vZ2Hl4vU6tXBeLWDf7425JOBEYkQEwONWZjGTPVH7fzp4dhMvnPrpQOKb06AzrbZuRrdzkhw== 13, 41 -- X-Received: by 10.70.138.37 with SMTP id qn5mr21011936pdb.118.1420779784129; 13, 41 -- Thu, 08 Jan 2015 21:03:04 -0800 (PST) 13, 41 -- X-Received: by 10.70.138.37 with SMTP id qn5mr21011914pdb.118.1420779783964; 13, 41 -- Thu, 08 Jan 2015 21:03:03 -0800 (PST) 13, 41 -- Received: from [192.168.1.10] (ppp118-209-72-8.lns20.mel4.internode.on.net. [118.209.72.8]) 13, 41 -- by mx.google.com with ESMTPSA id xl12sm5889037pac.41.2015.01.08.21.03.01 13, 41 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 41 -- Thu, 08 Jan 2015 21:03:03 -0800 (PST) 13, 41 -- Message-ID: {54AF60F6.6060807-at-monash.edu} 13, 41 -- Date: Fri, 09 Jan 2015 16:02:46 +1100 13, 41 -- From: Matthew Weyland {matthew.weyland-at-monash.edu} 13, 41 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 13, 41 -- MIME-Version: 1.0 13, 41 -- To: Microscopy-at-microscopy.com 13, 41 -- CC: chilan.ngo-at-ucla.edu 13, 41 -- Subject: Re: viaWWW:Cross-section Preparation of mesoporous silica 13, 41 -- References: {201501090039.t090dA9c006083-at-ns.microscopy.com} 13, 41 -- In-Reply-To: {201501090039.t090dA9c006083-at-ns.microscopy.com} 13, 41 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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ealname - Francoise Marga Email - fmarga-at-modernmeadow.com ORGANIZATION - Modern Meadow EDUCATION - Graduate College LOCATION - Brooklyn, NY, USA SUBJECT_OF_QUESTION - SEM in NYC QUESTION - Hi,
Our company would like to look at our samples by SEM. We need to go up x15,000 to visualize collagen fibers. As a business, we have trouble to find a SEM accessible to a private company. Does anyone know a facility or a private service in our area (Brooklyn, NY) that could help us. Thanks for your help. Kind regards,
Francoise
==============================Original Headers============================== 6, 31 -- From oshel1pe-at-cmich.edu Mon Jan 12 15:53:10 2015 6, 31 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0CLrAld023845 6, 31 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2015 15:53:10 -0600 6, 31 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 6, 31 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0CLrAee031293 6, 31 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2015 16:53:10 -0500 6, 31 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 6, 31 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 6, 31 -- (TLS) id 14.3.195.1; Mon, 12 Jan 2015 16:53:04 -0500 6, 31 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 6, 31 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 6, 31 -- 14.3.195.1; Mon, 12 Jan 2015 16:53:05 -0500 6, 31 -- Message-ID: {54B44244.2030207-at-cmich.edu} 6, 31 -- Date: Mon, 12 Jan 2015 16:53:08 -0500 6, 31 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 6, 31 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 31 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 31 -- MIME-Version: 1.0 6, 31 -- To: micro {microscopy-at-microscopy.com} 6, 31 -- Subject: Ask-A-Microscopist: SEM service available in Brooklyn NY area for 6, 31 -- collagen fibers? 6, 31 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 31 -- Content-Transfer-Encoding: 7bit 6, 31 -- X-Originating-IP: [141.209.2.100] 6, 31 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 31 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 6, 31 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 6, 31 -- X-CanItPRO-Stream: default 6, 31 -- X-Canit-Stats-ID: 02ND9Rax7 - 0460a495d384 - 20150112 6, 31 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Microscope Society of NE Ohio (MSNO)
Title-Subject: [Filtered] MSNO 2015 meeting
Message: MSNO 2015 Winter Meeting
MSNO 2015 Winter Meeting - Wednesday, March 4th, 2015, 3:00 Â 8:30 p.m. at Cleveland Museum of Natural History (1 Wade Oval Drive, University Circle, Cleveland, OH 44106)
Lillian A. Kuri from Cleveland Foundation will give a lecture on "ClevelandÂs Greater University Circle Initiative" and John Hemsath - Retired Director of Theater Operations will give a lecture on "The History of Playhouse Square"
Registration including dinner will be $20 for MSNO members, $25 for non-members and $5 for student members, $10 for student non-members. Preregistration is available at http://www.msneo.org/meetings.html, or registration and payment at the door will also be available (additional 5$ for all). Preregistration is required so we can get a good head count.
Please see more detail at https://www.facebook.com/MicroscopySociety/photos/pcb.631540963642067/631540420308788/?type=1&theater
Please contact nxa157-at-case.edu for more information.
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==============================Original Headers============================== 21, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 13 19:27:08 2015 21, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 21, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0E1R8Vr007935 21, 17 -- for {microscopy-at-microscopy.com} ; Tue, 13 Jan 2015 19:27:08 -0600 21, 17 -- Message-ID: {54B5C5EC.2020506-at-microscopy.com} 21, 17 -- Date: Tue, 13 Jan 2015 19:27:08 -0600 21, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 21, 17 -- MIME-Version: 1.0 21, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 17 -- Subject: viaWWW:MSNO 2015 meeting 21, 17 -- References: {201501132040.t0DKeu7t003559-at-ns.microscopy.com} 21, 17 -- In-Reply-To: {201501132040.t0DKeu7t003559-at-ns.microscopy.com} 21, 17 -- X-Forwarded-Message-Id: {201501132040.t0DKeu7t003559-at-ns.microscopy.com} 21, 17 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] Research Fellow in Scanning Transmission Electron Microscopy Imaging and Simulations of Interfaces in Light Alloys
Message: The following position is currently available:
Research Fellow in Scanning Transmission Electron Microscopy Imaging and Simulations of Interfaces in Light Alloys
Department of Materials Engineering, Monash University, Australia
Application closing date: 15 February 2015
http://jobs.monash.edu.au/jobDetails.asp?sJobIDs=530367 or http://users.monash.edu/~bourgeoi/Open-Positions.html
A/Prof. Laure Bourgeois Associate Professor - Microscope Manager Monash Centre for Electron Microscopy -- Department of Materials Engineering Building 81, 10 Innovation Walk Monash University, VIC 3800, Australia Tel: +61-(0)3-9905-5368 -- Fax: +61-(0)3-9905-3600 www.mcem.monash.edu.au users.monash.edu/~bourgeoi
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==============================Original Headers============================== 23, 18 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 14 06:56:33 2015 23, 18 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 23, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0ECuW5c012967 23, 18 -- for {microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 06:56:33 -0600 23, 18 -- Message-ID: {54B66780.9040002-at-microscopy.com} 23, 18 -- Date: Wed, 14 Jan 2015 06:56:32 -0600 23, 18 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 18 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 23, 18 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 23, 18 -- MIME-Version: 1.0 23, 18 -- To: microscopyListServer-Forward {microscopy-at-microscopy.com} 23, 18 -- Subject: viaWWW:Research Fellow in Scanning Transmission Electron Microscopy 23, 18 -- Imaging and Simulations of Interfaces in Light Alloys 23, 18 -- References: {201501140517.t0E5Hpqe000633-at-ns.microscopy.com} 23, 18 -- In-Reply-To: {201501140517.t0E5Hpqe000633-at-ns.microscopy.com} 23, 18 -- X-Forwarded-Message-Id: {201501140517.t0E5Hpqe000633-at-ns.microscopy.com} 23, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 23, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: grottigni-at-areialab.com Name: Rottigni V. Guglielmo
Organization: AREIA LABORATOIRES -NORMANDY - FRANCE
I use a Cressington 108 Carbon Coater to make carbon strata on polycarbonate filters, following ISO 13794 (the filters, after coating, are used to filter suspensions containing asbestos fibers), but _ while mantaining the same conditions (usually 4.5 V qnd three shots of " seconds each), the quantity of carbon deposed vary very much, and randomly. Sometimes I obtain 20 nm, sometimes only 10. Is there someone using the same Cressington Coater who may give me some advice qbout the best way to obtain carbon films of constant thickness?
Thanks in advance
Guglielmo Rottigni AREIA LABORATOIRES www.areialab.com Bourgtheroulde-Infreville Normandy FRANCE
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==============================Original Headers============================== 17, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 14 06:57:13 2015 17, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 17, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0ECvDS8013604 17, 17 -- for {microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 06:57:13 -0600 17, 17 -- Message-ID: {54B667A9.6030105-at-microscopy.com} 17, 17 -- Date: Wed, 14 Jan 2015 06:57:13 -0600 17, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 17, 17 -- MIME-Version: 1.0 17, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 17 -- Subject: viaWWW:CRESSINGTON 108 CARBON COATER 17, 17 -- References: {201501141113.t0EBDl6J007676-at-ns.microscopy.com} 17, 17 -- In-Reply-To: {201501141113.t0EBDl6J007676-at-ns.microscopy.com} 17, 17 -- X-Forwarded-Message-Id: {201501141113.t0EBDl6J007676-at-ns.microscopy.com} 17, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Electron Microscopy Facility of the Campus Science Support Facilities (Vienna, Austria) and NexÂpeÂrion - Solutions for Electron Microscopy (Vienna, Austria) are jointly organising an international
Advanced Course on Cryo-Electron Tomography Vienna, Austria, Europe June 6â7, 2015: Optional Pre-Course June 8â12, 2015: Main Course
for students, post-doctoral staff, scientists, and group leaders from academia and industry. While this course targets an advanced audience pre-existing knowledge in electron tomography, data processing and/or Cryo-TEM), a weekend pre-course will be offered for less experienced participants to catch-up.
The main part of the course will cover
Immersion freezing for cryo-electron tomography Cryo-CLEM Low-dose data collection with SeriÂalEM Processing of low-dose tilt series with IMOD Modelling and interpretation of cryo-electron tomography data SubtoÂmoÂgram averaging with PEET
Instructors and Organisers
Dr. Thomas Heuser, Campus Science Support Facilities, Vienna, Austria Dr. Johanna HÜÜg, University of Gothenburg, Sweden Dr. David MasÂtronarde, University of Colorado, Boulder, United States Dr. ReinÂhard Rachel, University of Regensburg, Germany Dr. Guenter Resch, NexÂpeÂrion e.U. - Solutions for Electron Microscopy, Vienna, Austria
Sponsored Guest Lectures
Dr. Andreas KasÂtenÂmĂźller, Gatan GmbH, Munich, Germany Dr. Ruwin Pandithage, Leica Microsystems, Vienna, Austria
For details about on-site infrastructure, the registration procedure and fees, see
http://www.nexperion.net/cryotomo2015
We are looking forward to seeing you there, best regards,
Guenter on behalf of all organizers
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
==============================Original Headers============================== 7, 25 -- From lists-at-nexperion.net Wed Jan 14 11:15:25 2015 7, 25 -- Received: from 503.hosttech.eu (503.hosttech.eu [62.112.145.5]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0EHFPQX027330 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 11:15:25 -0600 7, 25 -- Received: from localhost (unknown [127.0.0.1]) 7, 25 -- by 503.hosttech.eu (Postfix) with ESMTP id ABEE66CA0D1 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 17:15:31 +0000 (UTC) 7, 25 -- X-Virus-Scanned: amavisd-new at example.com 7, 25 -- Received: from 503.hosttech.eu ([127.0.0.1]) 7, 25 -- by localhost (503.hosttech.eu [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id Lyccsxea2nV6 for {Microscopy-at-microscopy.com} ; 7, 25 -- Wed, 14 Jan 2015 18:15:16 +0100 (CET) 7, 25 -- Received: from aventine (chello062178180023.17.14.vie.surfer.at [62.178.180.23]) 7, 25 -- by 503.hosttech.eu (Postfix) with ESMTPSA id 645226CA0CD 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 18:15:16 +0100 (CET) 7, 25 -- Date: Wed, 14 Jan 2015 18:15:07 +0100 7, 25 -- From: Guenter Resch {lists-at-nexperion.net} 7, 25 -- To: Microscopy-at-microscopy.com 7, 25 -- Subject: Advanced Course on Cryo-Electron Tomography, Vienna, June 2015 7, 25 -- Message-ID: {20150114181507.7de1b05d-at-aventine} 7, 25 -- X-Mailer: Claws Mail 3.9.3 (GTK+ 2.24.23; x86_64-pc-linux-gnu) 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=UTF-8 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0EHFPQX027330 ==============================End of - Headers==============================
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Email: javaidqazi-at-kemet.com Name: Javaid Qazi
Organization: Kemet Electronics
Title-Subject: [Filtered] Feed back on SEM-EDS systems
Message: All,
I am planning to buy a SEM with EDS. I would like to get feedback from the group.
I am interested in knowing pros and cons between the two Hitachi SU3500 and JEOL IT300LV to decide which one to get.
In terms of EDS which systems are better, any recommendation both in terms of detector size and software usage.
The equipment will typically be used by 3-4 users who do have experience using the SEM-EDS.
I will take the feedback offline.
Thanks for your help.
Javaid
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I just ordered the same OSRAM 103 W/2 bulbs I've been using for years in a Zeiss HBO 100 unit. The cathode (top) of all 3 bulbs that arrived is too wide to fit into the HBO 100's clamp (bottom cathode is OK). Comparing with the old bulb I ordered 9 months ago, the anode is indeed wider now than before. Has anyone else received OSRAM 103 W/2 bulbs recently and were the dimensions OK? I got mine from Bulbtronics.com. How about with the similar USHIO 103D?
Thanks, Esteban
==============================Original Headers============================== 3, 27 -- From g.esteban.fernandez-at-gmail.com Fri Jan 16 00:50:12 2015 3, 27 -- Received: from mail-yh0-f51.google.com (mail-yh0-f51.google.com [209.85.213.51]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0G6oCVi015337 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2015 00:50:12 -0600 3, 27 -- Received: by mail-yh0-f51.google.com with SMTP id a41so9377388yho.10 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=20120113; 3, 27 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 27 -- bh=SyoELpsT7M2u4ScUjeNrLiZTvRKY3Z3nYrn4nN+BzFQ=; 3, 27 -- b=XSWNgtdH9aMRBoVL0o7jHomBH2jJBMZsoBizofA7LtITLCx4PAKNW+3VVj50XgYMuJ 3, 27 -- 4MzA8r+kjFSnt9jRt77lahEtck8uIsSi7xUIovwgukBDndF4g1HOC2n7Y5FgPQ3Ay1L5 3, 27 -- S1gjU+m84I50zkdLvv+GbI16G0bU/zmCd+uOL19STlE8rPIVOwVyCzCmdOfVn1RSRm6i 3, 27 -- xFn1NVNHqPkhw/C+JAyjvfC3lStfcHa2uUV2vt2swrvDGDszFEJKyNyUR7Q49B3orfI2 3, 27 -- 6b80ndL49mB8LT76GicMVhTUTvqUxkEMmSAG8cfBEsIurV+wJt9v8q6Kc5d2GHAfgPFX 3, 27 -- dTYQ== 3, 27 -- MIME-Version: 1.0 3, 27 -- X-Received: by 10.170.61.202 with SMTP id d193mr9936210ykd.32.1421391010895; 3, 27 -- Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- Received: by 10.170.174.134 with HTTP; Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- Date: Thu, 15 Jan 2015 22:50:10 -0800 3, 27 -- Message-ID: {CAB_3w78mthi57-Kc4L_dx=pbkoRhmjZYqodbpm7RLLQ6aXMEuw-at-mail.gmail.com} 3, 27 -- Subject: Changed dimensions of OSRAM mercury bulb 103 W/2? 3, 27 -- From: "G. Esteban Fernandez" {g.esteban.fernandez-at-gmail.com} 3, 27 -- To: Confocal Microscopy List {CONFOCALMICROSCOPY-at-lists.umn.edu} , 3, 27 -- microscopy-at-microscopy.com 3, 27 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Does anyone have a TEM digital imaging plate system - the Ditabis system, any others? - sitting around no longer being used? And looking to get rid of it?
A couple have come up on the list over the years, as we all move to digital cameras, but have a unique imaging experiment system we've been playing around with and these plates might serve really well.
No need to clog the list so just contact me offline.
Thank you!
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
The specifications of the bulb including dimensions of the anode are given at this link file:///C:/Users/phillipst/Downloads/ZMP_56327.pdf - it would be interesting to see whether the match your old bulb or the new one. Let us know the result since I also use those bulbs.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: g.esteban.fernandez-at-gmail.com [mailto:g.esteban.fernandez-at-gmail.com] Sent: Friday, January 16, 2015 12:51 AM To: Phillips, Thomas E.
Hi everyone,
I just ordered the same OSRAM 103 W/2 bulbs I've been using for years in a Zeiss HBO 100 unit. The cathode (top) of all 3 bulbs that arrived is too wide to fit into the HBO 100's clamp (bottom cathode is OK). Comparing with the old bulb I ordered 9 months ago, the anode is indeed wider now than before. Has anyone else received OSRAM 103 W/2 bulbs recently and were the dimensions OK? I got mine from Bulbtronics.com. How about with the similar USHIO 103D?
Thanks, Esteban
==============================Original Headers============================== 3, 27 -- From g.esteban.fernandez-at-gmail.com Fri Jan 16 00:50:12 2015 3, 27 -- Received: from mail-yh0-f51.google.com (mail-yh0-f51.google.com [209.85.213.51]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0G6oCVi015337 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2015 00:50:12 -0600 3, 27 -- Received: by mail-yh0-f51.google.com with SMTP id a41so9377388yho.10 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=20120113; 3, 27 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 27 -- bh=SyoELpsT7M2u4ScUjeNrLiZTvRKY3Z3nYrn4nN+BzFQ=; 3, 27 -- b=XSWNgtdH9aMRBoVL0o7jHomBH2jJBMZsoBizofA7LtITLCx4PAKNW+3VVj50XgYMuJ 3, 27 -- 4MzA8r+kjFSnt9jRt77lahEtck8uIsSi7xUIovwgukBDndF4g1HOC2n7Y5FgPQ3Ay1L5 3, 27 -- S1gjU+m84I50zkdLvv+GbI16G0bU/zmCd+uOL19STlE8rPIVOwVyCzCmdOfVn1RSRm6i 3, 27 -- xFn1NVNHqPkhw/C+JAyjvfC3lStfcHa2uUV2vt2swrvDGDszFEJKyNyUR7Q49B3orfI2 3, 27 -- 6b80ndL49mB8LT76GicMVhTUTvqUxkEMmSAG8cfBEsIurV+wJt9v8q6Kc5d2GHAfgPFX 3, 27 -- dTYQ== 3, 27 -- MIME-Version: 1.0 3, 27 -- X-Received: by 10.170.61.202 with SMTP id d193mr9936210ykd.32.1421391010895; 3, 27 -- Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- Received: by 10.170.174.134 with HTTP; Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- Date: Thu, 15 Jan 2015 22:50:10 -0800 3, 27 -- Message-ID: {CAB_3w78mthi57-Kc4L_dx=pbkoRhmjZYqodbpm7RLLQ6aXMEuw-at-mail.gmail.com} 3, 27 -- Subject: Changed dimensions of OSRAM mercury bulb 103 W/2? 3, 27 -- From: "G. Esteban Fernandez" {g.esteban.fernandez-at-gmail.com} 3, 27 -- To: Confocal Microscopy List {CONFOCALMICROSCOPY-at-lists.umn.edu} , 3, 27 -- microscopy-at-microscopy.com 3, 27 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
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Email: rowland-at-matsys.com Name: Rod Rowland
Organization: MATSYS, Inc.
Title-Subject: [Filtered] JEOL 6100
Message: We have a JEOL 6100 which needs the magnification power supply board replaced. Our chiller stopped working the other day for about an hour and the mag. power supply got so hot half of the wires melted. We are trying to repair it in house but it's not looking good. If anyone can suggest a source for either repair it would be greatly appreciated. The JEOL 6100 model is SM111040-154. The power supply model is SM111040-7A.
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My name is Evan Francis and I'm a recruiter with Schafer Corporation. We're looking to hire a qualified Electron Microscopy Senior Scientist at our lab out in the Sunol, CA area. When you get this, if you're interested, please give me a call at 703-516-6051 or shoot me an email at evan.francis-at-schafercorp.com. Here is the position description on our company website:
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==============================Original Headers============================== 13, 17 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 16 19:24:03 2015 13, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0H1O2Z1025118 13, 17 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2015 19:24:03 -0600 13, 17 -- Message-ID: {54B9B9B3.8080702-at-microscopy.com} 13, 17 -- Date: Fri, 16 Jan 2015 19:24:03 -0600 13, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 13, 17 -- MIME-Version: 1.0 13, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 17 -- Subject: viaWWW:Senior Electron Microscopy Scientist Needed! 13, 17 -- References: {201501161648.t0GGmMCA007483-at-ns.microscopy.com} 13, 17 -- In-Reply-To: {201501161648.t0GGmMCA007483-at-ns.microscopy.com} 13, 17 -- X-Forwarded-Message-Id: {201501161648.t0GGmMCA007483-at-ns.microscopy.com} 13, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
According with my expérience you'll have to change this power supply because it's hard to repair when such problem occurs. This part contains many power transistors and, for this reason, it's water cooled. The most common accident occurs when water flow inside hose and power is switch off. The gradiant between room temperature and cooling water temperature may causes condensation of water (from air humidity) on the surface of power supply and finally destructive short-circuits when power supply is switch on. Week-end and hollydays are dangerous periods where the SEM can be shut down but not water cooling supply. Normally there is a small detector that shut off the board when temperature exceed 70°; it seems this security has not worked for your SEM or this was shorted on the past of your SEM (it's very easy to do from outside of the board and very easy to forget later). Probably JEOL has not such parts in spare because the JSM 6100 is rather old. Another solution is to find this part from a scrapped 6100 (there is today one 6100 on EBay for 12000 dollars). Last solution is to change all the broken parts of the board (there is probably many). This is possible for a good electronics engineer if you can supply him the diagramms. Hope this is help.
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique ŕ balayage et microanalyse 2 rue de la Houssiničre BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
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==============================Original Headers============================== 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Tue Jan 20 04:12:09 2015 8, 25 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0KAC8Mf031824 8, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2015 04:12:08 -0600 8, 25 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 8, 25 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id t0KAC6b2024513 8, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2015 11:12:06 +0100 8, 25 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 8, 25 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0KAC5OV018825 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT) 8, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2015 11:12:06 +0100 8, 25 -- Message-ID: {54BE2A2B.208-at-univ-nantes.fr} 8, 25 -- Date: Tue, 20 Jan 2015 11:12:59 +0100 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 8, 25 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 8, 25 -- MIME-Version: 1.0 8, 25 -- To: microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] viaWWW:JEOL 6100 repair 8, 25 -- References: {201501170141.t0H1frSX017421-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201501170141.t0H1frSX017421-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-Miltered: at b-mail2 with ID 54BE29F6.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 8, 25 -- X-j-chkmail-Enveloppe: 54BE29F6.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Dear all, anybody out there who has a Usermanual for the Zeiss DSM 960 SEM available in PDF?
Best wishes, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: jlribas-at-us.es Name: Juan Luis Ribas
Organization: Microscopy Facility. Univ of Seville. Spain
Title-Subject: [Filtered] Libra 120. Problem with screens not moving
Message: Good morning everybody, We are having problems with the moving of the small and large screens in our Libra 120. Sometimes (even 3-4 a day) the small screen stay in up position and the large screen in down position and the system (Wintem or iTEM) hangs. That means that neither through the left touch pannel (Small screen or M8) nor the software Wintem buttom have any action when pressed. Also, there is no way to get them back trough iTEM. The rest of the buttons and actions in Wintem are fully functional. The only way to unblock them is to go to off with Libra and on again. Even going to standby has no action.
Someone with more experience in Libra120 handling has experimented this situation before? Thank you very much in advance. Best regards
Juan Luis Ribas
Microscopy Facility. Univ of Seville. Spain
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] ESEM
Message: Hi!
Does any one is having service manual of 'FEI' ESEM Quanta 3D
Rashmi
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Email: SLC6-at-Lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh Microscopy School 2015
Message: Now accepting registrations for the 45th annual Lehigh Microscopy School which will be held June 7-12, 2015. All courses, lecturers, and instrument suppliers will be together for what promises to be a phenomenal week. Course offerings include: Scanning Electron Microscopy and X-ray Microanalysis  Introduction to SEM and EDS for the New Operator  Focused Ion Beam Instrumentation and Applications  Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD Data  Quantitative X-ray Microanalysis: Problem Solving using EDS and WDS Techniques  Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register and pay in full by April 14 for an Early Bird Discount! Contact: Sharon Coe (sharon.coe-at-Lehigh.edu or 610-758-5133). See www.lehigh.edu/microscopy for prices.
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Email: 13qw9-at-queensu.ca Name: Jason Wang
Organization: Queen's University
Title-Subject: [Filtered] how to stream the in-situ TEM video to another computer?
Message: Hello all, what are the normal ways you use for streaming or capturing the in-situ TEM videos by another computer? Since the computer directly connected to TEM is not allowed to install any other software on it, we are not able to take the in-situ video easily. So does connecting to another computer having a Video Capture Card will solve this problem? Thanks for you time!
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Email: gciambrone-at-comcast.net Name: Gary Ciambrone
Organization: Foothill College
Title-Subject: [Filtered] Stage position upon microscope moving/storage
Message: I'm in the process of beginning to teach some community college students the proper way to use a microscope. I have used microscopes in the past and have always moved the stage down away from the objectives (after swinging the low power objective into place) for moving or storing the scope. But then I have run across some information online that implies that this is incorrect. My question therefore is: what is the recommended position for the stage when moving or storing a microscope? Should the stage be moved as far from the objectives as possible, or should the stage be moved all the way to the top position after the low power objective is swung into place? Thank you very much.
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*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
} realname - hatem alharbi } Email - hatem.alharbi-at-my.mcphs.edu } ORGANIZATION - mcphs } EDUCATION - Graduate College } LOCATION - boston,MA,USA } SUBJECT_OF_QUESTION - flow cytometry + microscopy } QUESTION - Do you have comparison between flow cytomety devices please } how many flow cytometry + microscopy devices there and what is the best one of them }
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Wed Jan 21 07:07:39 2015 5, 32 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0LD7do4005074 5, 32 -- for {microscopy-at-microscopy.com} ; Wed, 21 Jan 2015 07:07:39 -0600 5, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 5, 32 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0LD7bdL003839 5, 32 -- for {microscopy-at-microscopy.com} ; Wed, 21 Jan 2015 08:07:37 -0500 5, 32 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 5, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.195.1; Wed, 21 Jan 2015 08:07:38 -0500 5, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 32 -- cas2.central.cmich.local (141.209.15.141) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.195.1; Wed, 21 Jan 2015 08:07:38 -0500 5, 32 -- Message-ID: {54BFA491.3090202-at-cmich.edu} 5, 32 -- Date: Wed, 21 Jan 2015 08:07:29 -0500 5, 32 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: micro {microscopy-at-microscopy.com} 5, 32 -- Subject: Re: Ask-A-Microscopist: any flow cytometers with microscopes? 5, 32 -- References: {831116267.1397.1421803102220.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 32 -- In-Reply-To: {831116267.1397.1421803102220.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 32 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit 5, 32 -- X-Originating-IP: [141.209.2.100] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 05NGB7B3x - cc2422988384 - 20150121 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Hooke College of Applied Sciences, located in Westmont, IL, is offering a Scanning Electron Microscopy short course March 23-27, 2015. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.
For further SEM training details and registration information, please follow the link below:
Chris Gorman | Admissions Specialist Hooke College of Applied Sciences, LLC . 850 Pasquinelli Drive . Westmont, IL 60559 P. 630.887.7100 | F. 630.887.7412 | www.hookecollege.com
==============================Original Headers============================== 8, 41 -- From CGorman-at-hookecollege.com Thu Jan 22 10:44:46 2015 8, 41 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 8, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0MGikow001847 8, 41 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 10:44:46 -0600 8, 41 -- X-ASG-Debug-ID: 1421945083-07bbd410cb57fb0001-4CH8be 8, 41 -- Received: from TMGEX2.tmg.mccrone.com ([192.168.101.54]) by spam.mccrone.com with ESMTP id TJB1hhP0WeDoLO5x for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 10:44:43 -0600 (CST) 8, 41 -- X-Barracuda-Envelope-From: CGorman-at-hookecollege.com 8, 41 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by 8, 41 -- TMGEX2.tmg.mccrone.com (192.168.101.74) with Microsoft SMTP Server (TLS) id 8, 41 -- 15.0.913.22; Thu, 22 Jan 2015 10:44:42 -0600 8, 41 -- Received: from TMGEX1.tmg.mccrone.com ([::1]) by TMGEX1.tmg.mccrone.com 8, 41 -- ([fe80::5c:a323:e462:160%14]) with mapi id 15.00.0913.011; Thu, 22 Jan 2015 8, 41 -- 10:44:42 -0600 8, 41 -- From: Christine Gorman {CGorman-at-hookecollege.com} 8, 41 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 8, 41 -- Subject: Scanning Electron Microscopy short course March 23-27, 2015 8, 41 -- Thread-Topic: Scanning Electron Microscopy short course March 23-27, 2015 8, 41 -- X-ASG-Orig-Subj: Scanning Electron Microscopy short course March 23-27, 2015 8, 41 -- Thread-Index: AdA2Yp7amkh3/6q5SL2qvUjXtDzI0g== 8, 41 -- Date: Thu, 22 Jan 2015 16:44:41 +0000 8, 41 -- Message-ID: {764a26b9fc6146c39685f7b7eeada80d-at-TMGEX1.tmg.mccrone.com} 8, 41 -- Accept-Language: en-US 8, 41 -- Content-Language: en-US 8, 41 -- X-MS-Has-Attach: 8, 41 -- X-MS-TNEF-Correlator: 8, 41 -- x-originating-ip: [192.168.101.154] 8, 41 -- Content-Type: text/plain; charset="iso-8859-1" 8, 41 -- MIME-Version: 1.0 8, 41 -- X-Barracuda-Connect: UNKNOWN[192.168.101.54] 8, 41 -- X-Barracuda-Start-Time: 1421945083 8, 41 -- X-Barracuda-URL: http://spam.mccrone.com:80/cgi-mod/mark.cgi 8, 41 -- X-Virus-Scanned: by bsmtpd at mccrone.com 8, 41 -- X-Barracuda-BRTS-Status: 1 8, 41 -- X-Barracuda-Spam-Score: 0.00 8, 41 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 8, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.14531 8, 41 -- Rule breakdown below 8, 41 -- pts rule name description 8, 41 -- ---- ---------------------- -------------------------------------------------- 8, 41 -- Content-Transfer-Encoding: 8bit 8, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0MGikow001847 ==============================End of - Headers==============================
Hello We cannot align the aperture in the JSM5600LV - SEM . Using the wobbler tool we cannot correct horizontal movement, in all 3 aperture positions.. Looks like the alignment point lies outside the aperture openings. The problem worsens with increasing tilt angle and affects the resolution (5K looks like 15K). I cleaned the upper column up to the aperture level and the aperture, but didn't help (it was not dirty anyway). However, last time I changed filament I noticed some little white crisps around the Wehnelt.orifice. Is it likely to have contamination of the lower parts of the column or somethig else? Any advise will be much appreciated Thanks yorgos
I don`t expect the dirt on the wehnelt aperture to be much of a problem, but better clean it away. Are you sure your final aperture strip (or do you have single apertures?) are not moving in the aperturestrip-holder? Did you check that the holder is perfectly clean? Since you say you have the same problem with all three aperture openings it might also be a problem with the astigmatism correction voltage going to the coils. If you have the schematics, you can measure this at the plug.
All the best, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 22.01.15 um 17:54 schrieb eikonika-at-otenet.gr: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello } We cannot align the aperture in the JSM5600LV - SEM . Using the wobbler tool } we cannot correct horizontal movement, in all 3 aperture positions.. Looks } like the alignment point lies outside the aperture openings. The problem } worsens with increasing tilt angle and affects the resolution (5K looks like } 15K). I cleaned the upper column up to the aperture level and the aperture, } but didn't help (it was not dirty anyway). However, last time I changed } filament I noticed some little white crisps around the Wehnelt.orifice. } Is it likely to have contamination of the lower parts of the column or } somethig else? Any advise will be much appreciated } Thanks } yorgos } } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.net www.aim.cat } ************************************ } } } ==============================Original Headers============================== } 4, 22 -- From eikonika-at-otenet.gr Thu Jan 22 10:50:05 2015 } 4, 22 -- Received: from echidna.otenet.gr (smtp-out33.otenet.gr [83.235.69.33]) } 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0MGo5JF005234 } 4, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 10:50:05 -0600 } 4, 22 -- Received: from pegasus (ppp-2-84-173-165.home.otenet.gr [2.84.173.165]) } 4, 22 -- by echidna.otenet.gr (ESMTP) with SMTP } 4, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 18:50:01 +0200 (EET) } 4, 22 -- Message-ID: {8879A4BBAFB44336B6364198CE575588-at-pegasus} } 4, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr} } 4, 22 -- To: {microscopy-at-microscopy.com} } 4, 22 -- Subject: JSM5600LV beam alignment problem } 4, 22 -- Date: Thu, 22 Jan 2015 18:49:58 +0200 } 4, 22 -- MIME-Version: 1.0 } 4, 22 -- Content-Type: text/plain; } 4, 22 -- format=flowed; } 4, 22 -- charset="iso-8859-1"; } 4, 22 -- reply-type=original } 4, 22 -- Content-Transfer-Encoding: 7bit } 4, 22 -- X-Priority: 3 } 4, 22 -- X-MSMail-Priority: Normal } 4, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 } 4, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 23 -- From stefan.diller-at-t-online.de Thu Jan 22 11:49:56 2015 9, 23 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0MHntHe009757 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 11:49:55 -0600 9, 23 -- Received: from fwd24.aul.t-online.de (fwd24.aul.t-online.de [172.20.26.129]) 9, 23 -- by mailout04.t-online.de (Postfix) with SMTP id 8F26A4822A5 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 18:49:54 +0100 (CET) 9, 23 -- Received: from mac-pro.local (GibinyZD8hrn4wQ7a8Fv1AMjC0QtMtGrE4MWt7NFC9WFer452eNbWxXhLjwIjxowEA-at-[91.58.164.206]) by fwd24.t-online.de 9, 23 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) 9, 23 -- esmtp id 1YELt6-2xMJvc0; Thu, 22 Jan 2015 18:49:48 +0100 9, 23 -- Message-ID: {54C1383B.7040405-at-t-online.de} 9, 23 -- Date: Thu, 22 Jan 2015 18:49:47 +0100 9, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 9, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 9, 23 -- MIME-Version: 1.0 9, 23 -- To: microscopy-at-microscopy.com 9, 23 -- Subject: Re: [Microscopy] JSM5600LV beam alignment problem 9, 23 -- References: {201501221654.t0MGs6df013480-at-ns.microscopy.com} 9, 23 -- In-Reply-To: {201501221654.t0MGs6df013480-at-ns.microscopy.com} 9, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- X-ID: GibinyZD8hrn4wQ7a8Fv1AMjC0QtMtGrE4MWt7NFC9WFer452eNbWxXhLjwIjxowEA 9, 23 -- X-TOI-MSGID: 4a043bc0-c1a2-444b-8ce9-49d94abc2aa4 ==============================End of - Headers==============================
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} Below is the result of your form, submitted on Thursday, January 22, 2015 at 03:33:32 PM. } } realname - Giovanni De Caro, MD } Email - neurostar-at-outlook.it } ORGANIZATION - Museo Scienze Naturali Montalbo´ } EDUCATION - Graduate College } LOCATION - Campobasso, Italy } SUBJECT_OF_QUESTION - Spot Insight QE digital camera } QUESTION - Hi all, } } I have acquired secondhand a SPOT Insight QE digital camera , model 4.2 , S/N 221276. The camera appears to be in good overall shape, it powers up and the fan goes; unfortunately I do not have the manual, the software and the data cable. Hs someone of you experience in operating this camera and can give me some hints about how to find the lacking components? The manual is available online on the Spot website, but I do not know if this is the monochrome or the color camera model; about the data cable, maybe it can be found on the web and , to end, maybe there is a shareware software that can be used to get pictures from this camera on my PC. } I look forward receiving answers form you and remain.
==============================Original Headers============================== 5, 33 -- From oshel1pe-at-cmich.edu Thu Jan 22 15:46:34 2015 5, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0MLkXRQ006442 5, 33 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 15:46:34 -0600 5, 33 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0MLkVF8001988 5, 33 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 16:46:31 -0500 5, 33 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 5, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 33 -- (TLS) id 14.3.195.1; Thu, 22 Jan 2015 16:46:30 -0500 5, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 33 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 5, 33 -- 14.3.195.1; Thu, 22 Jan 2015 16:46:30 -0500 5, 33 -- Message-ID: {54C16FB6.3080200-at-cmich.edu} 5, 33 -- Date: Thu, 22 Jan 2015 16:46:30 -0500 5, 33 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 33 -- MIME-Version: 1.0 5, 33 -- To: micro {microscopy-at-microscopy.com} 5, 33 -- Subject: Re: Ask-A-Microscopist: manual, software, and cables for Spot Insight 5, 33 -- QE camera 5, 33 -- References: {1784974025.5285.1421958813550.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 33 -- In-Reply-To: {1784974025.5285.1421958813550.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 33 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 5, 33 -- Content-Transfer-Encoding: 8bit 5, 33 -- X-Originating-IP: [141.209.2.100] 5, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 33 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 33 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 33 -- X-CanItPRO-Stream: default 5, 33 -- X-Canit-Stats-ID: 02NH9Kvts - faea7a0ad411 - 20150122 5, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
May I suggest you take out the aperture strip and see if you can align the instrument to your satisfaction. The problem may be in another part of the column resulting in an apparent aperture problem. I too do not expect the dirt on the wehnelt to be a real problem.
Try this and let us know what happens. You will need to use small spot sizes to obtain a reasonable image quality in the test.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: 22 January 2015 17:51 To: protrain-at-emcourses.com
X-from: huixin.xiu-at-googlemail.com
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Email: huixin.xiu-at-googlemail.com Name: Helen Xiu
Organization: FEI
Title-Subject: [Filtered] chirality identification using TEM tomography
Message: Dear all,
We would like to identify the chirality of their spiral material using TEM tomography in a FEI Tecnai F20 with Explore 3D software. After TEM tomography acquisition, we used Inspect3D 3.1 to reconstruct the data, however, we found using different direction (Y direction or Z direction) to reconstruct volume, we got opposite chirality of their spiral. We compared with the chirality got from SEM image, finding that reconstructing along Y direction gives the right chirality, but as far as I know, Inspect3D4.0 will reconstruct the volume along z direction as a default. Can anybody share your expertise with tomography reconstruction or any related experience? Many thanks!
Best regards, Helen
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==============================Original Headers============================== 19, 17 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 23 07:24:27 2015 19, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 19, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0NDOR0j026794 19, 17 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2015 07:24:27 -0600 19, 17 -- Message-ID: {54C24B8B.4040608-at-microscopy.com} 19, 17 -- Date: Fri, 23 Jan 2015 07:24:27 -0600 19, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 19, 17 -- MIME-Version: 1.0 19, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 17 -- Subject: viaWWW:chirality identification using TEM tomography 19, 17 -- References: {201501230637.t0N6bd0b020124-at-ns.microscopy.com} 19, 17 -- In-Reply-To: {201501230637.t0N6bd0b020124-at-ns.microscopy.com} 19, 17 -- X-Forwarded-Message-Id: {201501230637.t0N6bd0b020124-at-ns.microscopy.com} 19, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 19, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I'm trying to analyze some STEM-EDS data acquired using a JEOL ARM-200CF. The data includes maps of several edges and is stored in ".img" and ".map" formats. I would like to import this data into the Cornell Spectrum Imager (CSI) program for analysis using PCA and other routines, but there doesn't seem to be a way to do so.
Can anyone offer advice on dealing with JEOL EDS data? Are there any viewers or ImageJ plugins that I could use to read/convert this data into the proper format?
Thanks for your help!
__________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: Monday, January 26, 2015 10:07 AM To: John Mardinly
Hello everyone,
I'm trying to analyze some STEM-EDS data acquired using a JEOL ARM-200CF. The data includes maps of several edges and is stored in ".img" and ".map" formats. I would like to import this data into the Cornell Spectrum Imager (CSI) program for analysis using PCA and other routines, but there doesn't seem to be a way to do so.
Can anyone offer advice on dealing with JEOL EDS data? Are there any viewers or ImageJ plugins that I could use to read/convert this data into the proper format?
Thanks for your help!
__________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
The University of Missouri - Columbia is looking for a new staff member for our Molecular Cytology Core (MCC) facility. The MCC is an instrumentation resource and service facility for all type of light microscopic imaging. Last year the MCC served approximately 200 clients from over 80 different labs located in 30 departments on our campus. Information on our facility can be found at http://biotech.rnet.missouri.edu/mcc/
The ideal candidate will have extensive experience in confocal microscopy. We are particularly interested in individuals with experience making use of FLIM or FLIM/FRET technology.
Job responsibilities include:
* Supervise and train users (faculty, postdoctoral fellows, and graduate students) in the use of sophisticated microscopy instruments and software. * Develop new protocols involving single photon and multi-photon confocal microscopy and FLIM/FRET applications. * Maintain instruments and a clean, safe and orderly work environment. * Assist in some service work for clients. * Prepare monthly billing statement; maintain inventory and order supplies, dispense materials to clients.
Although some applicants to this position may hold a PhD degree, it is not a requirement for those with significant experience in a core or imaging lab. To apply for the job, visit http://hrs.missouri.edu/find-a-job/staff/index.php and search for "Research Specialist II" - the job ID number is 15116. If you have questions, you can contact either the MCC's Director, Dr. Tom Phillips (phillipst-at-missouri.edu) or the core's Associate Director, Dr. Alexander Jurkevic (jurkevica-at-missouri.edu). The job will remain open until a suitable candidate is found.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax)
==============================Original Headers============================== 7, 30 -- From PhillipsT-at-missouri.edu Mon Jan 26 17:36:22 2015 7, 30 -- Received: from um-nip4-missouri-out.um.umsystem.edu (um-nip4-missouri-out.um.umsystem.edu [198.209.49.177]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0QNaMiu021944 7, 30 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2015 17:36:22 -0600 7, 30 -- X-IronPort-Anti-Spam-Filtered: true 7, 30 -- X-IronPort-Anti-Spam-Result: A2HJBQDizsZU/9SeoM9AFwODBlJZBMZBhXECgRpDAQEBAQEDeoI+OAgEHQITWQEBBDpNAgIBKhQQFhwlAQEEEwiIJA03zxYBhFIBCgEBAQEBARsEjWeBXDgRgwWBEwWOboNLgg2FFIpzhX4iggCBbm8BgUN+AQEB 7, 30 -- X-IPAS-Result: A2HJBQDizsZU/9SeoM9AFwODBlJZBMZBhXECgRpDAQEBAQEDeoI+OAgEHQITWQEBBDpNAgIBKhQQFhwlAQEEEwiIJA03zxYBhFIBCgEBAQEBARsEjWeBXDgRgwWBEwWOboNLgg2FFIpzhX4iggCBbm8BgUN+AQEB 7, 30 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 7, 30 -- by um-nip4-exch-relay.um.umsystem.edu with ESMTP; 26 Jan 2015 17:36:21 -0600 7, 30 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.75]) by 7, 30 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0210.002; 7, 30 -- Mon, 26 Jan 2015 17:36:21 -0600 7, 30 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 7, 30 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 7, 30 -- Subject: Staff position in LM core 7, 30 -- Thread-Topic: Staff position in LM core 7, 30 -- Thread-Index: AdA5v5Zrxzj78aj5TSClRg6cPC++CQAARUIw 7, 30 -- Date: Mon, 26 Jan 2015 23:36:20 +0000 7, 30 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DE7023734-at-UM-MBX-T02.um.umsystem.edu} 7, 30 -- References: {CB463A8DE3499A4CA204087E4EEB363DE70235FE-at-UM-MBX-T02.um.umsystem.edu} 7, 30 -- In-Reply-To: {CB463A8DE3499A4CA204087E4EEB363DE70235FE-at-UM-MBX-T02.um.umsystem.edu} 7, 30 -- Accept-Language: en-US 7, 30 -- Content-Language: en-US 7, 30 -- X-MS-Has-Attach: 7, 30 -- X-MS-TNEF-Correlator: 7, 30 -- x-originating-ip: [128.206.81.115] 7, 30 -- Content-Type: text/plain; charset="us-ascii" 7, 30 -- MIME-Version: 1.0 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0QNaMiu021944 ==============================End of - Headers==============================
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Email: sameesh-at-uga.edu Name: Sam Gonzalez
Organization: University of Georgia
Title-Subject: [Filtered] Fixation of mitochondria
Message: Okay I am very confused about how to go about fixing a sample of isolated mitochondria for TEM.
All the procedures seem to be very vague about "washing the pellet". To me, this implies resuspending the pellet in buffer and then spinning it back down. But the procedures I have looked at never mention the g force for this step, but does specify the time So I would assume just use the g force of the last centrifuge step performed, but this step involves addition of agarose solution to enrobe the pellet, so it seems like this would be a distinct and unrelated step.
Also, the addition of agarose is never mentioned again, so would I add it to the buffer for the washing steps, assuming washing involves more centrifugation?
Alternatively, does washing the pellet just mean inundating the pellet with buffer and then pouring it off? If that is the case, how do i do this for 20 minutes? Just let the buffer sit on the pellet for 20 minutes then pour it off?
Thanks, Sam Very confused masters student
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 26 18:57:44 2015 15, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0R0vhbh011871 15, 17 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2015 18:57:44 -0600 15, 17 -- Message-ID: {54C6E288.9040005-at-microscopy.com} 15, 17 -- Date: Mon, 26 Jan 2015 18:57:44 -0600 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:Fixation of mitochondria 15, 17 -- References: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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We have a custom holder for the JEOL 2100 but have been having problems with insertion and extraction. The holder does not get "sucked in" by the vacuum after the first and second rotations. It actually requires a little pushing, and sometimes extraction is very difficult, requiring more force than standard holder. The puzzling thing is that the holder works perfectly in a JEOL 2010F.
The holder manufacturer has rechecked all physical dimensions of the holder and the o-rings, finding them within tolerance. JEOL also has taken back the goniometer on repeated occasions but has not found a solution. Oddly, a loaner goniometer JEOL installs when they take ours away, happens to work without problems.
Last incident we had, the vacuum was breached upon holder extraction. While pulling the holder, the outer gasket on the goniometer was extracted too. What is the most possible cause for this? We see wear marks on the alignment pin of the holder.
Thanks!
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==============================Original Headers============================== 16, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 26 18:58:43 2015 16, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0R0whxX012825 16, 17 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2015 18:58:43 -0600 16, 17 -- Message-ID: {54C6E2C3.9010209-at-microscopy.com} 16, 17 -- Date: Mon, 26 Jan 2015 18:58:43 -0600 16, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 16, 17 -- MIME-Version: 1.0 16, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 17 -- Subject: viaWWW:Holder Insertion/Extraction problems JEOL 2100 16, 17 -- References: {201501261431.t0QEVwfb004278-at-ns.microscopy.com} 16, 17 -- In-Reply-To: {201501261431.t0QEVwfb004278-at-ns.microscopy.com} 16, 17 -- X-Forwarded-Message-Id: {201501261431.t0QEVwfb004278-at-ns.microscopy.com} 16, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
It sounds like a goniometer problem. Wear marks on the alignment pin is not good because the inner tube of the goniometer can be scratched. This tube is a rotating part that moves with the holder and a scuffing is possible between this tube and the internal wall of the goniometer. Scratchs on the inner tube can weak the vacuum sealing during the movement of the holder. If this hypothesis is the good one it's necessary to remove this tube (It's a job for JEOL engineer) to get it into shape of the goniometer wall (by soft abrasion with Pikal for example). If there is scratchs this inner tube must be replaced. This is a tricky job and expensive part. Each vacuum leak during holder push/pull movement is bad experience for the ion pump. Hope this is help.
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique ŕ balayage et microanalyse 2 rue de la Houssiničre BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
Le 27/01/2015 02:13, microscopylistserver-noreply-at-microscopy.com a écrit : } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } X-from: robernal-at-u.northwestern.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } please copy both robernal-at-u.northwestern.edu as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } } Email: robernal-at-u.northwestern.edu } Name: Rodrigo Bernal } } Organization: Northwestern University } } Title-Subject: [Filtered] Holder Insertion/Extraction problems JEOL } 2100 } } Message: Hello All, } } We have a custom holder for the JEOL 2100 but have been having } problems with insertion and extraction. The holder does not get } "sucked in" by the vacuum after the first and second rotations. It } actually requires a little pushing, and sometimes extraction is very } difficult, requiring more force than standard holder. The puzzling } thing is that the holder works perfectly in a JEOL 2010F. } } The holder manufacturer has rechecked all physical dimensions of the } holder and the o-rings, finding them within tolerance. JEOL also has } taken back the goniometer on repeated occasions but has not found a } solution. Oddly, a loaner goniometer JEOL installs when they take } ours away, happens to work without problems. } } Last incident we had, the vacuum was breached upon holder extraction. } While pulling the holder, the outer gasket on the goniometer was } extracted too. What is the most possible cause for this? We see wear } marks on the alignment pin of the holder. } } Thanks! } } Login Host: 158.130.72.175 Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Tue Jan 27 02:30:50 2015 7, 26 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0R8Un4L031783 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 02:30:49 -0600 7, 26 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 7, 26 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id t0R8UlIj013046; 7, 26 -- Tue, 27 Jan 2015 09:30:47 +0100 7, 26 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 7, 26 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0R8UkfC018245 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT); 7, 26 -- Tue, 27 Jan 2015 09:30:46 +0100 7, 26 -- Message-ID: {54C74CB6.3070307-at-univ-nantes.fr} 7, 26 -- Date: Tue, 27 Jan 2015 09:30:46 +0100 7, 26 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 7, 26 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 7, 26 -- MIME-Version: 1.0 7, 26 -- To: microscopy-at-microscopy.com, robernal-at-u.northwestern.edu 7, 26 -- Subject: Re: [Microscopy] viaWWW:Holder Insertion/Extraction problems JEOL 7, 26 -- 2100 7, 26 -- References: {201501270113.t0R1DlfP005080-at-ns.microscopy.com} 7, 26 -- In-Reply-To: {201501270113.t0R1DlfP005080-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-Miltered: at b-mail2 with ID 54C74CB7.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 7, 26 -- X-j-chkmail-Enveloppe: 54C74CB7.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Liquid agarose is added to make processing easier. Processing some samples such as cell suspensions and organelles can be tricky, especially when it a very small quantity, as the majority of the sample gets lost during all the washing steps - plus it can get very hard to pellet them in the thick embedding resins. Adding liquid agarose in the earlier stages - then leaving it to cool so that it sets hard - embeds the samples in a larger, gel like pellet that can then be processed through as if it were a larger sample - without the need for centrifugation at each step.
When I process organelles, I usually fix in primary fixative, wash in buffer, centrifuge it down into a good pellet, remove as much supernatant as possible, then add a small drop of warm (not too hot) agarose (3%), making sure there is no air bubbles between it and the pellet, leave in a warm waterbath for 10 minutes to allow the agarose to infuse with the pellet, then move to the fridge until the agarose is completely set. I then carefully remove the agarose/sample from the tube, and if necessary, use a razor blade to cut it into 1mm2 pieces for further processing. You should then have some nice cubes of agarose/sample (or maybe just a small smear of sample in the tip if you're processing organelles or a difficult sample) that you can process through without the need for further spinning.
Sometimes, despite all my best efforts, the sample pellet gets left behind in the Eppendorf when I remove the set agarose gel. In this instance, I carefully slip the pellet onto a glass slide, and sandwich it between the agarose that way. If anyone has any helpful hints to combat this, it would be much appreciated!
Hope that makes more sense Sam?
Nat
Miss Natalie Allcock CBS Electron Microscopy Facility College of Medicine, Biological Sciences and Psychology Adrian Building University of Leicester LE1 7RH
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: 27 January 2015 01:21 To: Allcock, Natalie S.
X-from: sameesh-at-uga.edu
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Email: sameesh-at-uga.edu Name: Sam Gonzalez
Organization: University of Georgia
Title-Subject: [Filtered] Fixation of mitochondria
Message: Okay I am very confused about how to go about fixing a sample of isolated mitochondria for TEM.
All the procedures seem to be very vague about "washing the pellet". To me, this implies resuspending the pellet in buffer and then spinning it back down. But the procedures I have looked at never mention the g force for this step, but does specify the time So I would assume just use the g force of the last centrifuge step performed, but this step involves addition of agarose solution to enrobe the pellet, so it seems like this would be a distinct and unrelated step.
Also, the addition of agarose is never mentioned again, so would I add it to the buffer for the washing steps, assuming washing involves more centrifugation?
Alternatively, does washing the pellet just mean inundating the pellet with buffer and then pouring it off? If that is the case, how do i do this for 20 minutes? Just let the buffer sit on the pellet for 20 minutes then pour it off?
Thanks, Sam Very confused masters student
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 26 18:57:44 2015 15, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0R0vhbh011871 15, 17 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2015 18:57:44 -0600 15, 17 -- Message-ID: {54C6E288.9040005-at-microscopy.com} 15, 17 -- Date: Mon, 26 Jan 2015 18:57:44 -0600 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:Fixation of mitochondria 15, 17 -- References: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 33, 32 -- From nsa2-at-leicester.ac.uk Tue Jan 27 04:58:07 2015 33, 32 -- Received: from bontebok.le.ac.uk (bontebok.le.ac.uk [143.210.16.36]) 33, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0RAw77T020747 33, 32 -- for {Microscopy-at-Microscopy.com} ; Tue, 27 Jan 2015 04:58:07 -0600 33, 32 -- Received: from host-133-195.it.le.ac.uk ([143.210.133.195]:64339 helo=exp-casht-i3.uol.le.ac.uk) 33, 32 -- by bontebok.le.ac.uk with esmtps (TLS1.0:RSA_AES_256_CBC_SHA1:256) 33, 32 -- (Exim 4.80) 33, 32 -- (envelope-from {nsa2-at-leicester.ac.uk} ) 33, 32 -- id 1YG3qO-0006zJ-2v 33, 32 -- for Microscopy-at-Microscopy.com; Tue, 27 Jan 2015 10:58:04 +0000 33, 32 -- Received: from EXP-DAG1-N2.uol.le.ac.uk ([::1]) by exp-casht-i3.uol.le.ac.uk 33, 32 -- ([143.210.133.195]) with mapi id 14.03.0195.001; Tue, 27 Jan 2015 10:58:05 33, 32 -- +0000 33, 32 -- From: "Allcock, Natalie S." {nsa2-at-leicester.ac.uk} 33, 32 -- To: "'Microscopy-at-Microscopy.com'" {Microscopy-at-Microscopy.com} 33, 32 -- Subject: RE: [Microscopy] viaWWW:Fixation of mitochondria 33, 32 -- Thread-Topic: [Microscopy] viaWWW:Fixation of mitochondria 33, 32 -- Thread-Index: AQHQOc+MQrqNW+MkVki+cYQMgojOwpzTwUFA 33, 32 -- Date: Tue, 27 Jan 2015 10:58:04 +0000 33, 32 -- Message-ID: {C31045C47F49444BA76584B3DC6020CE16C1BFC7-at-exp-dag1-n2.uol.le.ac.uk} 33, 32 -- References: {201501270121.t0R1L5ql019062-at-ns.microscopy.com} 33, 32 -- In-Reply-To: {201501270121.t0R1L5ql019062-at-ns.microscopy.com} 33, 32 -- Accept-Language: en-GB, en-US 33, 32 -- Content-Language: en-US 33, 32 -- X-MS-Has-Attach: 33, 32 -- X-MS-TNEF-Correlator: 33, 32 -- x-originating-ip: [143.210.155.106] 33, 32 -- Content-Type: text/plain; charset="us-ascii" 33, 32 -- MIME-Version: 1.0 33, 32 -- X-UoL-Id: 6cee7a9a2660ae1862e174211234a0d7-at-1YG3qO-0006zJ-2v-at-bontebok.le.ac.uk 33, 32 -- Content-Transfer-Encoding: 8bit 33, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0RAw77T020747 ==============================End of - Headers==============================
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Email: alice-at-rms.org.uk Name: Alice Pyper
Organization: Royal Microscopical Society
Title-Subject: [Filtered] RMS Electron Microscopy Spring School
Message: The Royal Microscopical Society Electron Microscopy Spring School is talking place at Leeds University, England, from 23rd to 27th March 2015.
The School offers education on the theory and practice of scanning and transmission electron microscopy. The course covers, imaging, diffraction, and chemical microanalysis, as well as the important area of specimen preparation. It consists of lectures, demonstrations and hands on practical periods, as well as offering plenty of opportunity to ask questions, and to arrange time for specific demonstrations. There are two basic streams, Physical and Engineering Sciences, and Life Sciences, with an additional stream for those wishing to concentrate on TEM.
The course is designed to suit both beginners and experienced microscopists from all areas of academia and industry.
We are pleased to have Steve Chapman give us an insight into a life in electron microscopy, by presenting the annual lecture which he has entitled "50 years and a Million Miles"
The contact for more information is alice-at-rms.org.uk
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 27 07:10:33 2015 15, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0RDAX27009286 15, 17 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 07:10:33 -0600 15, 17 -- Message-ID: {54C78E49.50806-at-microscopy.com} 15, 17 -- Date: Tue, 27 Jan 2015 07:10:33 -0600 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: microscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:RMS Electron Microscopy Spring School 15, 17 -- References: {201501271005.t0RA5prE020071-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201501271005.t0RA5prE020071-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201501271005.t0RA5prE020071-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Does anyone have a PDF of the Leica RM2065 operator's manual? We need to set up and use our old unit but the manual is missing.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 11, 26 -- From lettj-at-ent.wustl.edu Tue Jan 27 10:35:22 2015 11, 26 -- Received: from wusm-pcf.wustl.edu (pcf-ironmail02.wusm-pcf.wustl.edu [128.252.17.82]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0RGZLsY031915 11, 26 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 10:35:21 -0600 11, 26 -- Received: from ([10.39.163.68]) 11, 26 -- by pcf-ironmail02.wusm-pcf.wustl.edu with ESMTP with TLS id 5THYGJ1.29579809; 11, 26 -- Tue, 27 Jan 2015 10:34:06 -0600 11, 26 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 11, 26 -- PCFHUB01.wusm-pcf.wustl.edu ([10.39.163.64]) with mapi id 14.03.0181.006; 11, 26 -- Tue, 27 Jan 2015 10:35:18 -0600 11, 26 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 11, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 26 -- Subject: LM -- Leica RM2065 rotary microtome 11, 26 -- Thread-Topic: LM -- Leica RM2065 rotary microtome 11, 26 -- Thread-Index: AdA6Tt7iq792OVPSQhGCrkbeEtJ8og== 11, 26 -- Date: Tue, 27 Jan 2015 16:35:17 +0000 11, 26 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E1353220129605-at-pcfmbx01.wusm-pcf.wustl.edu} 11, 26 -- Accept-Language: en-US 11, 26 -- Content-Language: en-US 11, 26 -- X-MS-Has-Attach: 11, 26 -- X-MS-TNEF-Correlator: 11, 26 -- x-originating-ip: [10.39.135.187] 11, 26 -- Content-Type: text/plain; charset="iso-8859-1" 11, 26 -- MIME-Version: 1.0 11, 26 -- Content-Transfer-Encoding: 8bit 11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0RGZLsY031915 ==============================End of - Headers==============================
My second question of the day: Does anyone have a PDF of the Reichert Knifemaker II? (I guess the Reichert-Jung 705202 is almost identical.)
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 12, 26 -- From lettj-at-ent.wustl.edu Tue Jan 27 16:51:27 2015 12, 26 -- Received: from wusm-pcf.wustl.edu (pcf-ironmail01.wusm-pcf.wustl.edu [128.252.17.163]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0RMpRAR025736 12, 26 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 16:51:27 -0600 12, 26 -- Received: from ([10.39.163.69]) 12, 26 -- by pcf-ironmail01.wusm-pcf.wustl.edu with ESMTP with TLS id 4THYGJ1.108462541; 12, 26 -- Tue, 27 Jan 2015 16:51:24 -0600 12, 26 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 12, 26 -- DRPCFHUB01.wusm-pcf.wustl.edu ([10.39.163.69]) with mapi id 14.03.0181.006; 12, 26 -- Tue, 27 Jan 2015 16:51:24 -0600 12, 26 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 12, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 26 -- Subject: TEM -- Reichert Knifemaker II 12, 26 -- Thread-Topic: TEM -- Reichert Knifemaker II 12, 26 -- Thread-Index: AdA6g3f4VMmJQJPIT0ufOKfkLLThhA== 12, 26 -- Date: Tue, 27 Jan 2015 22:51:23 +0000 12, 26 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E135322012A76A-at-pcfmbx01.wusm-pcf.wustl.edu} 12, 26 -- Accept-Language: en-US 12, 26 -- Content-Language: en-US 12, 26 -- X-MS-Has-Attach: 12, 26 -- X-MS-TNEF-Correlator: 12, 26 -- x-originating-ip: [10.39.135.187] 12, 26 -- Content-Type: text/plain; charset="iso-8859-1" 12, 26 -- MIME-Version: 1.0 12, 26 -- Content-Transfer-Encoding: 8bit 12, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0RMpRAR025736 ==============================End of - Headers==============================
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Email: neurostar-at-outlook.it Name: Giovanni De Caro
Organization: Museo Scienze Naturali Montalbo´
Title-Subject: [Filtered] Spot microscope camera - help needed
Message: Hi, I am the scientific director of a no profit Science Museum for youngsters based in Southern Italy (http://web.tiscali.it/exploratorium). We have been given an old SPOT Insight QE color digital camera , model 4.2 , S/N 221276 (Diagnostic Instruments inc.) and we would like to use it for our educational programs. The camera came to us with its power supply, it powers up fine and the fan runs, the only problem is that we did not get the data cable and the proprietary PCI card to run it. We did contact Diagnostic Instruments but they asked about 2000 USD for the card and the cable, so we are looking for a different solution. May you help us? Are there universal PCI cards that can be modified to work with this camera? Thank you for your kind help, I look forward receiving your reply and remain Giovanni De Caro, MD Italy
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 27 20:03:08 2015 14, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S2383w015214 14, 17 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 20:03:08 -0600 14, 17 -- Message-ID: {54C84358.1060506-at-microscopy.com} 14, 17 -- Date: Tue, 27 Jan 2015 20:03:04 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:Spot microscope camera - help needed 14, 17 -- References: {201501272104.t0RL4pUM023977-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201501272104.t0RL4pUM023977-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201501272104.t0RL4pUM023977-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I wouldnât be surprised if it is a pretty generic RS-422 parallel interface like AIA. You might talk to the guys at EDT (Engineering Design Team, Inc), they make interfaces for a lot of cameras.
There is a card on ebay that is probably for your camera, search fir "Diagnostic Instruments Inc P/N 0457â and it should come up. Make them an offer.
-Jerry
} On Jan 27, 2015, at 6:21 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } SPOT Insight QE
==============================Original Headers============================== 7, 36 -- From jerry.biehler-at-gmail.com Tue Jan 27 20:34:31 2015 7, 36 -- Received: from mail-ig0-f180.google.com (mail-ig0-f180.google.com [209.85.213.180]) 7, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S2YV8v002997 7, 36 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 20:34:31 -0600 7, 36 -- Received: by mail-ig0-f180.google.com with SMTP id b16so8562202igk.1 7, 36 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 18:34:30 -0800 (PST) 7, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 36 -- d=gmail.com; s=20120113; 7, 36 -- h=content-type:mime-version:subject:from:in-reply-to:date:cc 7, 36 -- :content-transfer-encoding:message-id:references:to; 7, 36 -- bh=TKgH1EZGDItrHMCw+Dh51WKysz+KSdaoYF9wG1wDhSs=; 7, 36 -- b=ELnHk9KlWP9I9T1RSK8vnrxBal4S5WvviLAtPk/wyeYfd8TtnzLbA+Ldi2tulKqvEP 7, 36 -- UnRkMaT0+OSBzXdlqLEkK4NvByAoImW/0HowbGzOkcpBOiJuXML4FplbYW6RArhdAUBw 7, 36 -- Lux2MK+vf/bMgHwiialf4akpAypdJYa4FchAQLxrx4hcGYj0+PQGiDWw/zGpjfwQa+63 7, 36 -- q2fbS0wAwwTuf4uv/Q3J87+OIXmauleEY8ab1TM4UU+TA8yaFAJd6sq25+5GZCn9mnaD 7, 36 -- a+P0m3bSCo0ZdnH2oWES2CrQuNeoeTkJqIAB9qcR15JGodzceXX//CKq+vM1rT89+Jhm 7, 36 -- Vn2g== 7, 36 -- X-Received: by 10.42.151.67 with SMTP id d3mr1178748icw.56.1422412470714; 7, 36 -- Tue, 27 Jan 2015 18:34:30 -0800 (PST) 7, 36 -- Received: from [192.168.1.126] (static-50-53-94-149.bvtn.or.frontiernet.net. [50.53.94.149]) 7, 36 -- by mx.google.com with ESMTPSA id t5sm8733284ign.12.2015.01.27.18.34.29 7, 36 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 7, 36 -- Tue, 27 Jan 2015 18:34:30 -0800 (PST) 7, 36 -- Content-Type: text/plain; charset=utf-8 7, 36 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.4\)) 7, 36 -- Subject: Re: [Microscopy] viaWWW:Spot microscope camera - help needed 7, 36 -- From: Jerry Biehler {jerry.biehler-at-gmail.com} 7, 36 -- In-Reply-To: {201501280221.t0S2LeE8002157-at-ns.microscopy.com} 7, 36 -- Date: Tue, 27 Jan 2015 18:34:28 -0800 7, 36 -- Cc: neurostar-at-outlook.it 7, 36 -- Message-Id: {CE776AD7-7A17-45CD-939C-E5223A4FCCC7-at-gmail.com} 7, 36 -- References: {201501280221.t0S2LeE8002157-at-ns.microscopy.com} 7, 36 -- To: microscopy-at-microscopy.com 7, 36 -- X-Mailer: Apple Mail (2.2070.4) 7, 36 -- Content-Transfer-Encoding: 8bit 7, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0S2YV8v002997 ==============================End of - Headers==============================
First, thanks to everyone for all the excellent advice I've received in the past on this list. I've got the rather unique task of running an SEM program accessible to the general public at a makerspace in Chicago, as a volunteer with no formal training and a shoestring budget. As far as I know, our circumstance is rather unique. There's no way I could have managed this long without all the help I've received here.
I managed to track down and repair the problem with wildly fluctuating vacuum readings (an extremely dirty Penning gauge) and the reason the vacuum was really bad after I fixed it (a leaking air admit solenoid.) But a bad shaft coupling on our Edwards E2M12 rotary pump was causing it to overheat and burn its oil, and, unfortunately, splattering said burnt oil up the rubber vacuum hose. I secured funding to get the pump overhauled, but now I need to know what to do with the hose. I figure it needs to be replaced, seeing as it reeks of burnt oil.
What I'm measuring seems to be about 7/8 I.D. and 1/4" wall thickness, so about 1 3/8" O.D. It's difficult to get a good reading as the hose ends were either clamped around something or stretched. Looking at http://www.pchemlabs.com/subcatagoryb.asp?pid=Pure-Gum-Rubber it looks like the gum rubber vacuum hose they sell jumps from 13/16" I.D. with 3/8" walls (listed as used for KF16) 1" with 1/2" walls (listed as used for KF25). The pump has a KF25 fitting, and the hose slips over a copper pipe in a concrete block as a vibration damper. But that size seems like it would be too large.
McMaster-Carr is local for us, so I checked http://www.mcmaster.com/#abrasion-resistant-gum-rubber-tubing/ and see vacuum rated red and tan tubing. (I have no idea what the color means. For reference, the tubing that came with the SEM was black.) The tan jumps from 3/4" with 5/8" walls to 1" with 1/2" walls. The red does come in 7/8" but it has 1/2" walls too. I'm not even sure if that would fit out the opening in the back of the SEM.
There's a good chance the vacuum hose is original to the scope, which is a Leica S430. Any help would be appreciated. Alternately, am I incorrect in assuming the hose should be replaced?
Thanks, Ryan
==============================Original Headers============================== 6, 16 -- From rdpierce-at-pobox.com Tue Jan 27 23:27:47 2015 6, 16 -- Received: from elna.mackenziegems.com (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S5RkTw024307 6, 16 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 23:27:46 -0600 6, 16 -- Received: from [127.0.0.1] (localhost [127.0.0.1]) 6, 16 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id 38A576E0A42 6, 16 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 23:27:44 -0600 (CST) 6, 16 -- Message-ID: {54C8734F.2000009-at-pobox.com} 6, 16 -- Date: Tue, 27 Jan 2015 23:27:43 -0600 6, 16 -- From: Ryan Pierce {rdpierce-at-pobox.com} 6, 16 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 6, 16 -- MIME-Version: 1.0 6, 16 -- To: microscopy-at-microscopy.com 6, 16 -- Subject: SEM: Vacuum hose source? 6, 16 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
good "standard" supplier is http://duniway.com/catalog/su-tubing-clamps.php (many components, not just the hose)
a cheap-cheap option is more work but you can make whatever diameter hose plus it can be easily compressed around a (much) smaller diameter tube since support spring is separate. Components: a) braided PVC of suitable diameter, either Home Depot etc. or McMaster Carr; b) continuous spring for supporting hose from collapsing, Again McMaster Carr.
tubing: http://www.mcmaster.com/#standard-high-pressure-pvc-tubing/=vnotog then select "clear" or from home page enter "High-Pressure PVC Tubing" in search line, then select "clear"
spring: http://www.mcmaster.com/#cut-to-length-springs/=vnp0wo then select "extension spring" or from home page enter "Cut-to-Length Extension Springs" stainless springs softer and won't rust. They sold in 20" pieces and will easily extend 10+ times. Springs made with wire 0.04" to 0.08" thick are best for diameters around 1".
3/4" to 1" ID vac. hose made in this fashion costs between $2 and $5 per foot depending on source of materials. It is transparent so oil accumulation can be seen before problems begin plus PVC will outlast rubber hands down. The oldest still in use I installed in 1998.
Use standard common hose-clams as long as they flat and at least close to 1/2" wide. Wire clamps not recommended.
Thin-wall-soft-not-braided low pressure PVC is even cheaper but less durable and unforgiving wrt vac. leaks at installation. Same longevity unless stressed.
For long straight connections use large diameter thin-wall copper tube, again from Home Depot, just ensure adequate cross-section for very long connections.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 1/28/2015 12:29 AM, rdpierce-at-pobox.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } First, thanks to everyone for all the excellent advice I've received in } the past on this list. I've got the rather unique task of running an SEM } program accessible to the general public at a makerspace in Chicago, as } a volunteer with no formal training and a shoestring budget. As far as I } know, our circumstance is rather unique. There's no way I could have } managed this long without all the help I've received here. } } I managed to track down and repair the problem with wildly fluctuating } vacuum readings (an extremely dirty Penning gauge) and the reason the } vacuum was really bad after I fixed it (a leaking air admit solenoid.) } But a bad shaft coupling on our Edwards E2M12 rotary pump was causing it } to overheat and burn its oil, and, unfortunately, splattering said burnt } oil up the rubber vacuum hose. I secured funding to get the pump } overhauled, but now I need to know what to do with the hose. I figure it } needs to be replaced, seeing as it reeks of burnt oil. } } What I'm measuring seems to be about 7/8 I.D. and 1/4" wall thickness, } so about 1 3/8" O.D. It's difficult to get a good reading as the hose } ends were either clamped around something or stretched. Looking at } http://www.pchemlabs.com/subcatagoryb.asp?pid=Pure-Gum-Rubber it looks } like the gum rubber vacuum hose they sell jumps from 13/16" I.D. with } 3/8" walls (listed as used for KF16) 1" with 1/2" walls (listed as used } for KF25). The pump has a KF25 fitting, and the hose slips over a copper } pipe in a concrete block as a vibration damper. But that size seems like } it would be too large. } } McMaster-Carr is local for us, so I checked } http://www.mcmaster.com/#abrasion-resistant-gum-rubber-tubing/ and see } vacuum rated red and tan tubing. (I have no idea what the color means. } For reference, the tubing that came with the SEM was black.) The tan } jumps from 3/4" with 5/8" walls to 1" with 1/2" walls. The red does come } in 7/8" but it has 1/2" walls too. I'm not even sure if that would fit } out the opening in the back of the SEM. } } There's a good chance the vacuum hose is original to the scope, which is } a Leica S430. Any help would be appreciated. Alternately, am I incorrect } in assuming the hose should be replaced? } } Thanks, } Ryan } } ==============================Original Headers============================== } 6, 16 -- From rdpierce-at-pobox.com Tue Jan 27 23:27:47 2015 } 6, 16 -- Received: from elna.mackenziegems.com (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) } 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S5RkTw024307 } 6, 16 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 23:27:46 -0600 } 6, 16 -- Received: from [127.0.0.1] (localhost [127.0.0.1]) } 6, 16 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id 38A576E0A42 } 6, 16 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 23:27:44 -0600 (CST) } 6, 16 -- Message-ID: {54C8734F.2000009-at-pobox.com} } 6, 16 -- Date: Tue, 27 Jan 2015 23:27:43 -0600 } 6, 16 -- From: Ryan Pierce {rdpierce-at-pobox.com} } 6, 16 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 } 6, 16 -- MIME-Version: 1.0 } 6, 16 -- To: microscopy-at-microscopy.com } 6, 16 -- Subject: SEM: Vacuum hose source? } 6, 16 -- Content-Type: text/plain; charset=utf-8; format=flowed } 6, 16 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
On Jan 27, 2015, at 9:43 PM, rdpierce-at-pobox.com wrote:
} First, thanks to everyone for all the excellent advice I've received } in } the past on this list. I've got the rather unique task of running an } SEM } program accessible to the general public at a makerspace in Chicago, } as } a volunteer with no formal training and a shoestring budget. As far } as I } know, our circumstance is rather unique. There's no way I could have } managed this long without all the help I've received here. } } I managed to track down and repair the problem with wildly fluctuating } vacuum readings (an extremely dirty Penning gauge) and the reason the } vacuum was really bad after I fixed it (a leaking air admit solenoid.) } But a bad shaft coupling on our Edwards E2M12 rotary pump was } causing it } to overheat and burn its oil, and, unfortunately, splattering said } burnt } oil up the rubber vacuum hose. I secured funding to get the pump } overhauled, but now I need to know what to do with the hose. I } figure it } needs to be replaced, seeing as it reeks of burnt oil. } } What I'm measuring seems to be about 7/8 I.D. and 1/4" wall thickness, } so about 1 3/8" O.D. It's difficult to get a good reading as the hose } ends were either clamped around something or stretched. Looking at } http://www.pchemlabs.com/subcatagoryb.asp?pid=Pure-Gum-Rubber it looks } like the gum rubber vacuum hose they sell jumps from 13/16" I.D. with } 3/8" walls (listed as used for KF16) 1" with 1/2" walls (listed as } used } for KF25). The pump has a KF25 fitting, and the hose slips over a } copper } pipe in a concrete block as a vibration damper. But that size seems } like } it would be too large. } } McMaster-Carr is local for us, so I checked } http://www.mcmaster.com/#abrasion-resistant-gum-rubber-tubing/ and see } vacuum rated red and tan tubing. (I have no idea what the color means. } For reference, the tubing that came with the SEM was black.) The tan } jumps from 3/4" with 5/8" walls to 1" with 1/2" walls. The red does } come } in 7/8" but it has 1/2" walls too. I'm not even sure if that would fit } out the opening in the back of the SEM. } } There's a good chance the vacuum hose is original to the scope, } which is } a Leica S430. Any help would be appreciated. Alternately, am I } incorrect } in assuming the hose should be replaced? } } Thanks, } Ryan
Dear Ryan, If you make a cut through the tubing, you should be able to measure ID and OD accurately. Yours, Bill
==============================Original Headers============================== 8, 35 -- From wtivol-at-sbcglobal.net Wed Jan 28 02:14:26 2015 8, 35 -- Received: from nm22-vm6.access.bullet.mail.gq1.yahoo.com (nm22-vm6.access.bullet.mail.gq1.yahoo.com [216.39.63.170]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S8EQKS001410 8, 35 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2015 02:14:26 -0600 8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1422432865; bh=XSzdTAvSYETMXTQ53+oUZDvC9ZbjugpUTZz3OgZm7Yo=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=BOlRe1orBdvJoLk327YPQGl57UODLpHKdmgYHPGf7wmUJRMr52Nvh9BAtSSLzpyEYYdVwCiFJbdCzoleNB54I0AnJg5YCngi5zoVy2v0a2S06OeANA2HxDAiom+05ksqvQk0mStCtxq2FyUpboKNquZEBaYNTPcK7tUchXXDZ9qpE/i3FcpqpZyxMCgY3IF8bcljYBo+uUhpcntTzDkoRDpHAMpFUvGmYZxyFUnTCnH00XsSKDBu9jDnGl1YbZCyEzPytNxj2v43cHxe6iTl0dtr/SjQSX4Jk394aBieFqrKojbw+MjmDkg9dbvKWnBEJnXnc+Iti/hh2fRiAxCWPQ== 8, 35 -- Received: from [216.39.60.166] by nm22.access.bullet.mail.gq1.yahoo.com with NNFMP; 28 Jan 2015 08:14:25 -0000 8, 35 -- Received: from [67.195.22.116] by tm2.access.bullet.mail.gq1.yahoo.com with NNFMP; 28 Jan 2015 08:14:25 -0000 8, 35 -- Received: from [127.0.0.1] by smtp111.sbc.mail.gq1.yahoo.com with NNFMP; 28 Jan 2015 08:14:25 -0000 8, 35 -- X-Yahoo-Newman-Id: 961722.18361.bm-at-smtp111.sbc.mail.gq1.yahoo.com 8, 35 -- X-Yahoo-Newman-Property: ymail-3 8, 35 -- X-YMail-OSG: uAw2yy0VM1kvy.V8wNyegRcz8oKO6QeJNCxCh8pBrV3.nZQ 8, 35 -- IHwWMCuJRbPrRbCxMg1xvVQIbSnH255HrAU.ySf8uyhIRpqAsTpb7iiclaxI 8, 35 -- oveBvtBY3lq7dB2BN4LMRFx9Ex.nKng8mLtanNyuP1bKC9HJr15G.8ZGiczi 8, 35 -- lncII7JxafjOKowUsug3Xfrh9GbTLySzKK3ZgFFWhZNXn8PAHHxbhpE2vJTb 8, 35 -- 0EQzqnXMmmIxIQcQxL5veHUBxewbwU3Cm5dzwz0uHtd41UeZ9BBtdrm.msFN 8, 35 -- QiUI8xlZ9u1y9Y18je9sWifDZeAN2G56jJ1BgNhkFlw1R.qDQbCrIbM6j_eW 8, 35 -- I3InAvv0k6p2TW8JTGPGwaxlgt8R2zOZBdFoRgYfUjfErwmHRGtEZnC_InP6 8, 35 -- eMOwTmPu8FWaJl5Ta_GK321M3.cXDq2IqvIbA8wuWhoT1dl0V_n1zsLCk_ER 8, 35 -- FtW4nRSqQG2VYp52TC42qCBeyxnZiBY9Kn4xriLnySZnon6PuMovrv7Z4piO 8, 35 -- Srf2arjBKvK5G4S86fzcyKNafbsgjG.zYUoZ3Bpk0CcOI6h7Fmjt_sWKlILK 8, 35 -- Rz5uRrHDwL16jo2nyrwOCsHZlg.HKWtC2Vn6vuCndCiF_ZUQ1wFhoHbHl2pY 8, 35 -- MwMHNA5rGoeRQm.m3Fn7f6muZSNL1ae2pHM4_XZdWj2EBiOuKjRcD59KGhmo 8, 35 -- vdhUg1AdL.YlGVzzYmOPPvrGIP4kanA-- 8, 35 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 8, 35 -- Message-Id: {84196C3C-87E7-4830-B1E7-3F5EF198B635-at-sbcglobal.net} 8, 35 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 8, 35 -- To: microscopy-at-microscopy.com 8, 35 -- In-Reply-To: {201501280543.t0S5hjYw006423-at-ns.microscopy.com} 8, 35 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 35 -- Content-Transfer-Encoding: 7bit 8, 35 -- Mime-Version: 1.0 (Apple Message framework v936) 8, 35 -- Subject: Re: [Microscopy] SEM: Vacuum hose source? 8, 35 -- Date: Wed, 28 Jan 2015 00:14:23 -0800 8, 35 -- References: {201501280543.t0S5hjYw006423-at-ns.microscopy.com} 8, 35 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Virginia Tech is seeking an Instrument specialist for its Nanoscale Characterization and Fabrication Laboratory. The selected candidate will: Operate instrumentation in the Nanoscale Characterization and Fabrication Laboratory. Maintain and operate the FEI Helios Nanolab 600 Focused Ion Beam (FIB) in the NCFL. Keep the laboratory up to date in the methods and techniques used with these instruments. Perform FIB analyses and provide assessments of the results, support individual research with training on the instruments and guidance on the techniques. Provide analytical service to industrial clients. Maintain logs of all equipment usage and provide info to accounting monthly for timely billing through the service center. Comply with all Environmental Health and Safety regulations for proper lab operation. Keep up to date in the field on current techniques and procedures. Provide demonstrations for classes and visitors, and assistance on other instruments as needed. Virginia Tech is an Equal Opportunity/Affirmative Action Institution.
For further information or to apply, please go to: https://listings.jobs.vt.edu/postings/54282
Thank you, Christopher Winkler, PhD Senior Research Associate Nanoscale Characterization and Fabrication Laboratory Virginia Tech
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Message: Does anyone have a manual for a JEOL hollow-cone diffraction unit? Specifically, it's labeled HCID 10 and is part of a JEOL 2010 TEM. Thanks in advance!
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Title-Subject: [Filtered] SAVE THE DATE: NESM's Annual Spring Meeting -at- Bruker!
Message: Dear fellow microscopists,
This is a reminder to SAVE THE DATE for the New England Society for MicroscopyÂs annual Spring Meeting, which will take place on Thursday, March 5th at Bruker Corporation in Billerica, MA. The meeting will consist of facility tours, a buffet dinner and two technical talks showcasing microscopy application and innovation in the materials and life sciences. Stay tuned  more information, including speaker abstracts and bios, will follow in the coming weeks.
We hope to see you in March!
Cheers, NESM Board
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Email: sniper711220-at-hotmail.com Name: Gary
Organization: University of Oxford
Title-Subject: [Filtered] Analysis of Pb in the SEM-EDS
Message: Hi I have several copper-tin-lead alloy standards with varying Pb contents(5, 10, 15, 20). I analyzed them by the SEM-EDS, but it keeps giving me lower Pb concentrations(around 2, 5, 7, 10 individually). The totals are good around 100 precents, but just inaccurate(tin is the same, bur Pb is lower and Cu is higher than their real values. I use the default internal calibration (leaded glass) to calibrate Pb element. It is known that Pb in the copper alloy will form a separate phase.
Have anyone encountered such a problem and how to solve it ？
Thanks Gary
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 30 07:02:53 2015 15, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0UD2rlK014299 15, 17 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2015 07:02:53 -0600 15, 17 -- Message-ID: {54CB80FD.8000304-at-microscopy.com} 15, 17 -- Date: Fri, 30 Jan 2015 07:02:53 -0600 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:Analysis of Pb in the SEM-EDS 15, 17 -- References: {201501300509.t0U59pqm004683-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201501300509.t0U59pqm004683-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201501300509.t0U59pqm004683-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
How flat is the surface? What is the phase distribution on a micro-scale? If the Pb is present as a second phase it might well be preferentially polished away changing the volume (and mass) fraction and skewing the results.
Also, EDS correction routines are written to process intensities from homogeneous analysis volumes. If you are trying to lower the magnification and scan an area, you will present the sum of the phase intensities to the analyzer. However, Pb probably had nothing to do in determining the interaction volume in the regions where the Cu and Sn are nor did the Cu and Sn x-rays undergo absorption by Pb atoms. Once in a while, EDS might give the right answer in such cases, but I would not count on it being the right.
BTW, the same issue would be true of WDS too. The matrix corrections are much the same. They assume a homogenous sample volume.
Warren
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, January 30, 2015 7:04 AM To: Straszheim, Warren E [BIOTC]
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Email: sniper711220-at-hotmail.com Name: Gary
Organization: University of Oxford
Title-Subject: [Filtered] Analysis of Pb in the SEM-EDS
Message: Hi I have several copper-tin-lead alloy standards with varying Pb contents(5, 10, 15, 20). I analyzed them by the SEM-EDS, but it keeps giving me lower Pb concentrations(around 2, 5, 7, 10 individually). The totals are good around 100 precents, but just inaccurate(tin is the same, bur Pb is lower and Cu is higher than their real values. I use the default internal calibration (leaded glass) to calibrate Pb element. It is known that Pb in the copper alloy will form a separate phase.
Have anyone encountered such a problem and how to solve it ？
Thanks Gary
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==============================Original Headers============================== 23, 47 -- From wesaia-at-iastate.edu Fri Jan 30 09:03:49 2015 23, 47 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2on0084.outbound.protection.outlook.com [65.55.169.84]) 23, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0UF3mSA003300 23, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Jan 2015 09:03:48 -0600 23, 47 -- Received: from BN1PR04MB551.namprd04.prod.outlook.com (10.141.65.148) by 23, 47 -- BN1PR04MB185.namprd04.prod.outlook.com (10.255.204.153) with Microsoft SMTP 23, 47 -- Server (TLS) id 15.1.75.20; Fri, 30 Jan 2015 15:03:43 +0000 23, 47 -- Received: from BN1PR04MB552.namprd04.prod.outlook.com (10.141.65.152) by 23, 47 -- BN1PR04MB551.namprd04.prod.outlook.com (10.141.65.148) with Microsoft SMTP 23, 47 -- Server (TLS) id 15.1.65.19; Fri, 30 Jan 2015 15:03:41 +0000 23, 47 -- Received: from BN1PR04MB552.namprd04.prod.outlook.com ([169.254.6.42]) by 23, 47 -- BN1PR04MB552.namprd04.prod.outlook.com ([169.254.6.53]) with mapi id 23, 47 -- 15.01.0065.013; Fri, 30 Jan 2015 15:03:41 +0000 23, 47 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 23, 47 -- To: "sniper711220-at-hotmail.com" {sniper711220-at-hotmail.com} 23, 47 -- CC: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 23, 47 -- Subject: RE: [Microscopy] viaWWW:Analysis of Pb in the SEM-EDS 23, 47 -- Thread-Topic: [Microscopy] viaWWW:Analysis of Pb in the SEM-EDS 23, 47 -- Thread-Index: AQHQPI1fprqTSG+pr02aw+LOK7/ARpzYwKsA 23, 47 -- Date: Fri, 30 Jan 2015 15:03:41 +0000 23, 47 -- Message-ID: {BN1PR04MB552DFACAA17225677F53FDCD7310-at-BN1PR04MB552.namprd04.prod.outlook.com} 23, 47 -- References: {201501301304.t0UD4J7D015082-at-ns.microscopy.com} 23, 47 -- In-Reply-To: {201501301304.t0UD4J7D015082-at-ns.microscopy.com} 23, 47 -- Accept-Language: en-US 23, 47 -- Content-Language: en-US 23, 47 -- X-MS-Has-Attach: 23, 47 -- X-MS-TNEF-Correlator: 23, 47 -- x-originating-ip: [129.186.227.4] 23, 47 -- authentication-results: hotmail.com; dkim=none (message not signed) 23, 47 -- header.d=none;hotmail.com; dmarc=none action=none header.from=iastate.edu; 23, 47 -- x-dmarcaction-test: None 23, 47 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID:(3005004);SRVR:BN1PR04MB551;UriScan:; 23, 47 -- x-exchange-antispam-report-test: UriScan:; 23, 47 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:;SRVR:BN1PR04MB551; 23, 47 -- x-forefront-prvs: 04724A515E 23, 47 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(13464003)(377454003)(87936001)(54606007)(40100003)(575784001)(86362001)(2656002)(325944007)(77156002)(62966003)(90282001)(122556002)(102836002)(15975445007)(2950100001)(54356999)(50986999)(76176999)(2501002)(33656002)(74316001)(75432002)(92566002)(64544003)(89122001)(110136001)(2900100001)(46102003)(54206007)(88552001)(19580395003)(76576001)(1720100001)(106116001)(66066001)(551984002)(2351001)(19580405001);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR04MB551;H:BN1PR04MB552.namprd04.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 23, 47 -- Content-Type: text/plain; charset="us-ascii" 23, 47 -- MIME-Version: 1.0 23, 47 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 30 Jan 2015 15:03:41.1922 23, 47 -- (UTC) 23, 47 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 23, 47 -- X-MS-Exchange-CrossTenant-id: 0347d89a-0174-4dd3-adeb-3339c89c35f5 23, 47 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN1PR04MB551 23, 47 -- X-Microsoft-Antispam: BCL:0;PCL:0;RULEID:;SRVR:BN1PR04MB185; 23, 47 -- X-OriginatorOrg: iastate.edu 23, 47 -- Content-Transfer-Encoding: 8bit 23, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0UF3mSA003300 ==============================End of - Headers==============================
I would not use the Pb in glass standard for this analysis. You should try to use a standard as close to your unknown composition. Pb in SiO2 is not close. I would try using one of you CuSnPb standards as the standard and see how the results turn out. You say you 'use the default internal calibration (leaded glass) to calibrate Pb element', did you use the same operating conditions? kV, take off angle. Also which lines for each element did you use? K L M? Be careful of background too.
wesaia-at-iastate.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } How flat is the surface? What is the phase distribution on a micro-scale? If the Pb is present as a second phase it might well be preferentially polished away changing the volume (and mass) fraction and skewing the results. } } Also, EDS correction routines are written to process intensities from homogeneous analysis volumes. If you are trying to lower the magnification and scan an area, you will present the sum of the phase intensities to the analyzer. However, Pb probably had nothing to do in determining the interaction volume in the regions where the Cu and Sn are nor did the Cu and Sn x-rays undergo absorption by Pb atoms. Once in a while, EDS might give the right answer in such cases, but I would not count on it being the right. } } BTW, the same issue would be true of WDS too. The matrix corrections are much the same. They assume a homogenous sample volume. } } Warren } } -----Original Message----- } X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Friday, January 30, 2015 7:04 AM } To: Straszheim, Warren E [BIOTC] } Subject: [Microscopy] viaWWW:Analysis of Pb in the SEM-EDS } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: sniper711220-at-hotmail.com } } } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both sniper711220-at-hotmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: sniper711220-at-hotmail.com } Name: Gary } } Organization: University of Oxford } } Title-Subject: [Filtered] Analysis of Pb in the SEM-EDS } } Message: Hi } I have several copper-tin-lead alloy standards with varying Pb contents(5, 10, 15, 20). } I analyzed them by the SEM-EDS, but it keeps giving me lower Pb concentrations(around 2, 5, 7, 10 individually). } The totals are good around 100 precents, but just inaccurate(tin is the same, bur Pb is lower and Cu is higher than their real values. } I use the default internal calibration (leaded glass) to calibrate Pb element. } It is known that Pb in the copper alloy will form a separate phase. } } Have anyone encountered such a problem and how to solve it ？ } } Thanks } Gary } } Login Host: 163.1.130.189 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } -- } =========================================== } Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. 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Message: I am looking to purchase a used Si(Li) EDS system for a JEOL 2010 (horizontal EDS port). It seems the vendors have abandoned this technology for the SDD, but these systems are beyond our budget justification for such an old scope without STEM. If you are interested in selling such a system, please contact me offline.
Rich
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==============================Original Headers============================== 12, 17 -- From microscopylistserver-noreply-at-microscopy.com Sun Feb 1 12:52:20 2015 12, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t11IqKWm031166 12, 17 -- for {microscopy-at-microscopy.com} ; Sun, 1 Feb 2015 12:52:20 -0600 12, 17 -- Message-ID: {54CE75E4.2090502-at-microscopy.com} 12, 17 -- Date: Sun, 01 Feb 2015 12:52:20 -0600 12, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 12, 17 -- MIME-Version: 1.0 12, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 17 -- Subject: viaWWW:Looking for a used EDS system 12, 17 -- References: {201502011739.t11HdYp2030471-at-ns.microscopy.com} 12, 17 -- In-Reply-To: {201502011739.t11HdYp2030471-at-ns.microscopy.com} 12, 17 -- X-Forwarded-Message-Id: {201502011739.t11HdYp2030471-at-ns.microscopy.com} 12, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: lamiller-at-illinois.edu Name: Lou Ann Miller
Organization: Materials Research Laboratory, University of Illinois
Title-Subject: [Filtered] Job opening for an experienced Electron Microscopist
Message: Research Scientist(s) position Please see the link below for the job information and to apply electronically online:
*Note for those unfamiliar with University jobs here, a common question: Is the job for only one year with the "12 month appointment"?
All academic positions at the University are appointed on a 9- or 12-month service basis, eligible for annual renewal based upon mutual agreement and the annual performance review process. This Academic Professional position is a regularly recurring staff scientist role not subject to grant funding. See http://ap.illinois.edu for more information.
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230
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==============================Original Headers============================== 23, 18 -- From microscopylistserver-noreply-at-microscopy.com Mon Feb 2 08:20:04 2015 23, 18 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 23, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t12EK3Qv002907 23, 18 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2015 08:20:04 -0600 23, 18 -- Message-ID: {54CF8793.2000503-at-microscopy.com} 23, 18 -- Date: Mon, 02 Feb 2015 08:20:03 -0600 23, 18 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 18 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 23, 18 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 23, 18 -- MIME-Version: 1.0 23, 18 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 23, 18 -- Subject: viaWWW:Job opening for an experienced Electron Microscopist - Univ. 23, 18 -- of Illinois 23, 18 -- References: {201502021411.t12EBfYo002745-at-ns.microscopy.com} 23, 18 -- In-Reply-To: {201502021411.t12EBfYo002745-at-ns.microscopy.com} 23, 18 -- X-Forwarded-Message-Id: {201502021411.t12EBfYo002745-at-ns.microscopy.com} 23, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 23, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I had several questions from folks on the list serve about another job posting. I did not see it yesterday, but it appears to be a resend of the ⌠maybe now 15-16 month ago email that somehow got retransmitted a while back.
Iâm sorry, I do not know where in cyber this came from, but there is no TEM job opening at MRL laboratory at this time. ( They updated our Lync system, perhaps it was somehow caught in that action)
Thanks to those who asked, so I became aware, and many apologies for those looking for a job, that this has resurfaced. We filled both spots a year ago.
Lou Ann
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230
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Email: lou.howell-at-live.com Name: Louise
Organization: Institute of Cancer Research
Title-Subject: [Filtered] advice on new camera for Zeiss Axioplan 2
Message: Dear all,
IÂm looking into upgrading some software for my Zeiss Axioplan 2 microscope (currently have very old SmartCapture software). We look at protein expression levels and also DNA damage foci and so something which could quantify these signals would be ideal. Also IÂm looking into buying a new camera compatible with the new software/microscope.
Any advice on the best camera and suitable software compatible with this microscope would be most gratefully received.
Thank you very much in advance.
Louise.
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==============================Original Headers============================== 17, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 4 07:13:59 2015 17, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 17, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t14DDwSX025651 17, 17 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2015 07:13:58 -0600 17, 17 -- Message-ID: {54D21B16.9060703-at-microscopy.com} 17, 17 -- Date: Wed, 04 Feb 2015 07:13:58 -0600 17, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 17, 17 -- MIME-Version: 1.0 17, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 17 -- Subject: viaWWW:advice on new camera for Zeiss Axioplan 2 17, 17 -- References: {201502041123.t14BNQT2023553-at-ns.microscopy.com} 17, 17 -- In-Reply-To: {201502041123.t14BNQT2023553-at-ns.microscopy.com} 17, 17 -- X-Forwarded-Message-Id: {201502041123.t14BNQT2023553-at-ns.microscopy.com} 17, 17 -- Content-Type: text/plain; charset=UTF-8; format=flowed 17, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: KSU
Title-Subject: [Filtered] Calibration of Oxford EDS.
Message: Hi, I have EDS analysis of Au Nanoparticle on Copper TEM grid using Oxford EDS.But I am getting lot of cu peaks and instead of Au peak. I am using INCA microanalysos suit version 4.15. Could anybody suggest me how to do calibration and What kind of std reference sample needed for calibration.?
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 4 16:56:49 2015 14, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t14MunNp028822 14, 17 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2015 16:56:49 -0600 14, 17 -- Message-ID: {54D2A3B1.8020804-at-microscopy.com} 14, 17 -- Date: Wed, 04 Feb 2015 16:56:49 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:Calibration of Oxford EDS. 14, 17 -- References: {201502042042.t14Kg36Z024862-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201502042042.t14Kg36Z024862-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201502042042.t14Kg36Z024862-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You are always going to see Cu under those circumstances. The amount of Cu metal in the grid is vast compared to the size of an Au nanoparticle. As your electron beam passes through the Au nanoparticle, some electrons will scatter and hit the grid producing fluorescence. There can also be electrons scattered from electron microscope components such as apertures as well â though this is less a problem these days than it used to be (depends on the age of your microscope).
If you arenât interested in Cu, I suggest you just ignore the peak. If you are interested in Cu in your nanoparticle, then producers such as EMS, Ted Pella and Ladd make a variety of grids out of a variety of elements and compounds so that you can almost certainly find some element you donât care about.
When avoiding Cu, Iâve had luck using beryllium, and silicon nitride.
For general calibration, a simple and very common approach is to use the Cliff-Lorimer method of k-factors: Cliff, G., & Lorimer, G. W. (1975). The Quantitative Analysis of Thin Specimens. Journal of Microscopy, 103(2), 203â207.
The Oxford software has this method built in and allows you to define those k-factors once you measure them (using the method from the paper above). The Oxford software also has some good guesses built in if you donât need very much accuracy.
I have also found it useful to extract peak areas from TEM/EDS spectra and process them myself. I first use fityk, Python, or the Oxford/Bruker softwares to fit peak areas. Then I use k-factors and apply a thickness correction using some software I wrote for myself (https://github.com/ZGainsforth/StoichiometryFitter/blob/master/Stoichiometry%20Fitter%20Screenshot.png) You might like a solution like this if you want to control every aspect of the analysis.
Hopefully that clears it up?
Zack
On Feb 4, 2015, at 3:14 PM, microscopylistserver-noreply-at-microscopy.com wrote:
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: KSU
Title-Subject: [Filtered] Calibration of Oxford EDS.
Message: Hi, I have EDS analysis of Au Nanoparticle on Copper TEM grid using Oxford EDS.But I am getting lot of cu peaks and instead of Au peak. I am using INCA microanalysos suit version 4.15. Could anybody suggest me how to do calibration and What kind of std reference sample needed for calibration.?
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==============================Original Headers============================== 32, 36 -- From zackg-at-berkeley.edu Wed Feb 4 17:43:44 2015 32, 36 -- Received: from mail-ie0-f169.google.com (mail-ie0-f169.google.com [209.85.223.169]) 32, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t14NhimD016685 32, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Feb 2015 17:43:44 -0600 32, 36 -- Received: by mail-ie0-f169.google.com with SMTP id rl12so6241285iec.0 32, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Feb 2015 15:43:43 -0800 (PST) 32, 36 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 32, 36 -- d=1e100.net; s=20130820; 32, 36 -- h=x-gm-message-state:from:content-type:content-transfer-encoding 32, 36 -- :subject:date:references:to:message-id:mime-version; 32, 36 -- bh=LJPiuRHVw6TQMIPgcC7byr6GC1byPI6/WZX6dN9FCTs=; 32, 36 -- b=ZZLbGeCO83B/liSo0DeOY6iNPJG6AYJPREPpWf3SHKmD8KFFcT6fir+Es7aB0HKYLm 32, 36 -- KJh1QZIZdw1gXu2LY978BbzwmaFvQ2Q3y3Es0UK5T3SwmYumFYenZNCBKwhSLc8z3rhn 32, 36 -- IZrGNaEvtTqu77sV18ZzapK52JspKNW3YDkQTbTz5oO7sEFF6fEzTX5xNjuvGnFnIwPl 32, 36 -- NnpKEHz2+Zs68I3ZKclKAnAKfc/57xXSkJiVfofDSKTjLA5WLz1BjdmJlnL0iNhJXmh0 32, 36 -- oiBFGqUUTYJHIGJ0NlHRC8EKsDEVHxBinULp52wcabZl8qwwq/rzQZsenGRBX/xq0nDt 32, 36 -- D+yw== 32, 36 -- X-Gm-Message-State: ALoCoQk3T0SVLC6HSYF1/vqqGbMNpZ8lmzYNmmFl0TkXoN69/8mND2sk6zll5QjwS96w968kQwhq 32, 36 -- X-Received: by 10.50.154.106 with SMTP id vn10mr27241541igb.49.1423093423558; 32, 36 -- Wed, 04 Feb 2015 15:43:43 -0800 (PST) 32, 36 -- Received: from continuum.home (pool-173-60-44-43.lsanca.fios.verizon.net. [173.60.44.43]) 32, 36 -- by mx.google.com with ESMTPSA id g15sm1628049ioi.22.2015.02.04.15.43.42 32, 36 -- for {Microscopy-at-microscopy.com} 32, 36 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 32, 36 -- Wed, 04 Feb 2015 15:43:42 -0800 (PST) 32, 36 -- From: Zack Gainsforth {zackg-at-berkeley.edu} 32, 36 -- Content-Type: text/plain; charset=utf-8 32, 36 -- Subject: Re: [Microscopy] viaWWW:Calibration of Oxford EDS. 32, 36 -- Date: Wed, 4 Feb 2015 15:43:41 -0800 32, 36 -- References: {64508464-990E-4EDC-8AF5-7CD62986884F-at-berkeley.edu} 32, 36 -- To: Microscopy-at-microscopy.com 32, 36 -- Message-Id: {4F29FE4E-AE92-44C3-97F3-BCF086A09908-at-berkeley.edu} 32, 36 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.6\)) 32, 36 -- X-Mailer: Apple Mail (2.2070.6) 32, 36 -- Content-Transfer-Encoding: 8bit 32, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t14NhimD016685 ==============================End of - Headers==============================
From mrsivonneemile-at-gmail.com Sat Feb 7 12:28:05 2015 Return-Path: {mrsivonneemile-at-gmail.com} Received: from n10.c03.server-system.net (n10.c03.server-system.net [72.47.224.10]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t17IS5kS008220; Sat, 7 Feb 2015 12:28:05 -0600 Received: from [146.71.103.85] (port=51998 helo=User) by n10.c03.server-system.net with esmtpa (Exim 4.80.1) (envelope-from {mrsivonneemile-at-gmail.com} ) id 1YKA6r-0007G7-S4; Sat, 07 Feb 2015 10:28:01 -0800 Reply-To: {mrsivonnemile-at-religious.com}
X-from: rashmi_mehata-at-yahoo.com
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] 'FEI' TEM HRTEM ESEM service Manuals
Message: HI
Our 'FEI' TEM, HRTEM and ESEM are not functioning for some time. Does anyone have the service manuals for this microscopes which will help us bring back these instruments into working conditions
Regards Rashmi
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Email: ian.maclaren-at-glasgow.ac.uk Name: Ian MacLaren
Organization: University of Glasgow
Title-Subject: [Filtered] Two Postdoc Positions - Glasgow and Oxford
Message: SCHOOL OF PHYSICS AND ASTRONOMY, UNIVERSITY OF GLASGOW DEPARTMENT OF MATERIALS, UNIVERSITY OF OXFORD
Two post-doctoral research positions in fast pixelated detectors for scanning transmission electron microscopy
Oxford: Research Associate, Grade 7 / Salary in the range: Ł30,434 to Ł33,242 p.a. / Vacancy ID: 117029
Applications are invited for two fixed-term (up to 36 months) postdoctoral positions (one at Glasgow and one at Oxford) in research associated with using fast pixelated detectors in STEM.
The overarching aim of the project is to use fast pixelated detectors to record the intensity as a function of scattering angle in the detector plane of a STEM, which is effectively a diffraction pattern. By recording each two-dimensional diffraction pattern as a function of probe position in a two-dimensional scan, a four-dimensional data set can be recorded that is the ultimate STEM imaging experiment. Such a rich dataset contains information about the phase shift that results from transmission, about the composition of the sample, the strain in the sample and the three-dimensional ordering in the sample. We propose to develop the methods to record this 4D data set, using fast pixelated detectors, and by developing an optimised direct-detection system, together with the methods to process such datasets to enable physically useful measurements to be made. Application areas include imaging of soft materials, detection of fields and charge transfer, separation of strain and compositional information, and measurement of three-dimensional crystallographic ordering.
The posts have been created through the funding by the EPSRC of a joint project between The Universities of Glasgow and Oxford on developing methods and applications associated with pixelated detectors in aberration-corrected scanning transmission electron microscopy.
The posts are available from 1 April 2015. By the start date, applicants should have a good first degree and completed PhD in Physics, Materials, Engineering, Chemistry or a related field. They should have experience with operating a STEM and a good understanding of its principles of operation, a high level of expertise in the theory of electron scattering and image formation in the electron microscope, and excellent IT skills including the use of electron microscopy specific software packages and an ability to write code using a suitable high-level language.
The closing date for applications is 11 March 2015 (Glasgow, midnight; Oxford, midday) with interviews planned for 24 March 2015 (in Glasgow). It is important that candidates submit applications to both Oxford and Glasgow (these can be identical applications) if they wish to be considered for both posts.
Informal enquiries about the Glasgow post may be directed to Dr Ian MacLaren, ian.maclaren-at-glasgow.ac.uk Informal enquiries about the Oxford post should be directed to Prof. Peter Nellist, peter.nellist-at-materials.ox.ac.uk
Glasgow Application Details:
Apply online at www.glasgow.ac.uk/jobs
Closing date: 11 March 2015. The School of Physics and Astronomy has been awarded Juno Champion status and also the Athena SWAN Silver Award.
The University has recently been awarded the Athena SWAN Institutional Bronze Award.
The University is committed to equality of opportunity in employment.
The University of Glasgow, charity number SC004401.
Oxford Application Details:
Applications for the Oxford post are to be made online (vacancy reference 117029). To apply for this role and for further details, including the job description and selection criteria, please click on the link below:
The Department of Materials is an Athena Swan silver award holder. Applications are particularly welcome from women and black and ethnic minority candidates, who are under-represented in research posts in the Department.
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Hello Everyone, I'm looking for suggestions about a beam sensitive sample.
I'm cutting thin sections with a cryomicrotome of HIPS, High Impact Polystyrene, that is toughened with polybutadiene. I'm cutting at -100 C because of the low TG of the polybutadiene and staining with Os. My thin sections are very beam sensitive, too sensitive to focus well and get a good image. I'm imaging at 80KV with 8800X magnification. If I spread out my beam to reduce beam damage I can't see my sample to focus and any pictures I take look crappy.
Any suggestions?
I've been thinking about trying carbon coated grids to help act as a heat sink as well as going to a larger TEM spot setting. I have read that lower KV can make the damage worse because each electron resides in the thin section longer and produced more heat. Is this a tried and true observation?
Thanks!!!
Frank
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==============================Original Headers============================== 9, 28 -- From frank_karl-at-ardl.com Mon Feb 9 12:15:49 2015 9, 28 -- Received: from cal1-mh775.smtproutes.com (cal1-mh775.smtproutes.com [208.70.91.141]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t19IFmQm020125 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 12:15:48 -0600 9, 28 -- X-Katharion-ID: 1423505727.99310.cal1-mh775 9, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by 9, 28 -- cal1-mh775.smtproutes.com [(192.69.16.141)] with ESMTP via TCP 9, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 09 Feb 2015 18:15:27 +0000 9, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 9, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 9 9, 28 -- Feb 2015 13:15:26 -0500 9, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 9, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 9, 28 -- {microscopy-at-microscopy.com} 9, 28 -- Date: Mon, 9 Feb 2015 13:15:26 -0500 9, 28 -- Subject: TEM and HIPS 9, 28 -- Thread-Topic: TEM and HIPS 9, 28 -- Thread-Index: AdBElGBOLrir6G9eQt6ILmdqm8wZ1g== 9, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C83443E1B-at-exchange2k7.ad.ardl.com} 9, 28 -- Accept-Language: en-US 9, 28 -- Content-Language: en-US 9, 28 -- X-MS-Has-Attach: 9, 28 -- X-MS-TNEF-Correlator: 9, 28 -- acceptlanguage: en-US 9, 28 -- Content-Type: text/plain; charset="us-ascii" 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t19IFmQm020125 ==============================End of - Headers==============================
You'll probably have better luck if you can put a thin carbon coating on top of your microtomed sample prior to examination in the TEM. A nanometer or less--just a few seconds in most carbon coaters--should minimize the charging and preserve your samples under the beam. Staining should also help stabilize the sample but apparently not in this case.
If you don't have a coater then you can try "cooking" the microtome sections in the TEM: go to a few thousand magnification, remove the condenser aperture and adjust your condenser lenses to the largest spot size (highest beam current), and spread the beam out to illuminate a large fraction of the thin section. Leave it in this condition, assuming the sample is not sputtering away, for a few minutes. Take a look at 10kx magnification and see if it's stable. If not, try again for a longer time. This may never stabilize the section but often helps in my experience.
We look at HIPS samples (albiet stained samples with a few angstroms of carbon coating) all the time at 200kV and 300kV and even run long STEM maps without issue. Try a higher accelerating voltage if your microscope is capable of it. The damage mechanisms are complicated in TEM but generally lower voltages increase interaction cross-section of the electron in the sample, leading to higher sample temperatures and radiolysis effects. Higher accelerating voltages minimize the interaction cross-section but lead to knock-on damage. Egerton, Li, and Malac wrote a paper titled, "Radiation Damage in the TEM and SEM" (Micron 35 (2004) 399-409) which can be a good starting point for understanding beam induced damage.
Good luck, Chris Senior Research Associate Nanoscale Characterization and Fabrication Laboratory Institute for Critical Technology and Applied Science Virginia Tech (540) 200-9511
On Mon, Feb 9, 2015 at 1:25 PM, {frank_karl-at-ardl.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Everyone, } I'm looking for suggestions about a beam sensitive sample. } } I'm cutting thin sections with a cryomicrotome of HIPS, High Impact Polystyrene, that is toughened with polybutadiene. I'm cutting at -100 C because of the low TG of the polybutadiene and staining with Os. } My thin sections are very beam sensitive, too sensitive to focus well and get a good image. I'm imaging at 80KV with 8800X magnification. If I spread out my beam to reduce beam damage I can't see my sample to focus and any pictures I take look crappy. } } Any suggestions? } } I've been thinking about trying carbon coated grids to help act as a heat sink as well as going to a larger TEM spot setting. I have read that lower KV can make the damage worse because each electron resides in the thin section longer and produced more heat. Is this a tried and true observation? } } Thanks!!! } } Frank } } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } } ==============================Original Headers============================== } 9, 28 -- From frank_karl-at-ardl.com Mon Feb 9 12:15:49 2015 } 9, 28 -- Received: from cal1-mh775.smtproutes.com (cal1-mh775.smtproutes.com [208.70.91.141]) } 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t19IFmQm020125 } 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 12:15:48 -0600 } 9, 28 -- X-Katharion-ID: 1423505727.99310.cal1-mh775 } 9, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by } 9, 28 -- cal1-mh775.smtproutes.com [(192.69.16.141)] with ESMTP via TCP } 9, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 09 Feb 2015 18:15:27 +0000 } 9, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by } 9, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 9 } 9, 28 -- Feb 2015 13:15:26 -0500 } 9, 28 -- From: Frank Karl {frank_karl-at-ardl.com} } 9, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" } 9, 28 -- {microscopy-at-microscopy.com} } 9, 28 -- Date: Mon, 9 Feb 2015 13:15:26 -0500 } 9, 28 -- Subject: TEM and HIPS } 9, 28 -- Thread-Topic: TEM and HIPS } 9, 28 -- Thread-Index: AdBElGBOLrir6G9eQt6ILmdqm8wZ1g== } 9, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C83443E1B-at-exchange2k7.ad.ardl.com} } 9, 28 -- Accept-Language: en-US } 9, 28 -- Content-Language: en-US } 9, 28 -- X-MS-Has-Attach: } 9, 28 -- X-MS-TNEF-Correlator: } 9, 28 -- acceptlanguage: en-US } 9, 28 -- Content-Type: text/plain; charset="us-ascii" } 9, 28 -- MIME-Version: 1.0 } 9, 28 -- Content-Transfer-Encoding: 8bit } 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t19IFmQm020125 } ==============================End of - Headers==============================
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Yes to both your questions. Putting the sections on carbon-coated grids will help with heat dissipation, and lowering the kV will increase beam damage because of more beam interactions with your specimens. We typically use a 10 ľm spot when looking at our thin sections, especially at lower mags. And spread the illumination. Maybe try 100 kV. But - how thick are your thin sections? If they're 100 - 120 nm, try cutting them 80 - 90 nm. Less beam energy deposition in the thinner sections. And of course, this being reality, if they're already this thin (or thinner), try 100 nm sections, as they'll be more robust, and maybe use the higher kV. Probably the carbon-coated grids will do the best, but changing kV is easy.
(Hey, it's all quantum - your sections are simultaneously in burst and unburst states, you just have to pick the ones that collapse to the unburst state when you observe them.)
Phil
} Hello Everyone, } I'm looking for suggestions about a beam sensitive sample. } } I'm cutting thin sections with a cryomicrotome of HIPS, High Impact Polystyrene, that is toughened with polybutadiene. I'm cutting at -100 C because of the low TG of the polybutadiene and staining with Os. } My thin sections are very beam sensitive, too sensitive to focus well and get a good image. I'm imaging at 80KV with 8800X magnification. If I spread out my beam to reduce beam damage I can't see my sample to focus and any pictures I take look crappy. } } Any suggestions? } } I've been thinking about trying carbon coated grids to help act as a heat sink as well as going to a larger TEM spot setting. I have read that lower KV can make the damage worse because each electron resides in the thin section longer and produced more heat. Is this a tried and true observation? } } Thanks!!! } } Frank } } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Mon Feb 9 12:48:03 2015 5, 32 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t19Ilvh6020004 5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 12:48:03 -0600 5, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 5, 32 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t19Ikoco029695; 5, 32 -- Mon, 9 Feb 2015 13:46:50 -0500 5, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 5, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.195.1; Mon, 9 Feb 2015 13:46:49 -0500 5, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.195.1; Mon, 9 Feb 2015 13:46:48 -0500 5, 32 -- Message-ID: {54D90098.1080308-at-cmich.edu} 5, 32 -- Date: Mon, 9 Feb 2015 13:46:48 -0500 5, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: {frank_karl-at-ardl.com} , micro {microscopy-at-microscopy.com} 5, 32 -- Subject: Re: [Microscopy] TEM and HIPS 5, 32 -- References: {201502091832.t19IWKIe004860-at-ns.microscopy.com} 5, 32 -- In-Reply-To: {201502091832.t19IWKIe004860-at-ns.microscopy.com} 5, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-Originating-IP: [141.209.2.100] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -0.40 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 05NOiKOIq - f51c085a9689 - 20150209 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Does anyone know of an anti-catalase primary antibody for post-label on HM20 sections? I have rabbit and mouse 2Ab. A researcher gave me the ABCAM anti-catalase antibody ab16731 but it was not tested for TEM and my results were negative.
Any suggestions or recommendations would be greatly appreciated.
Karen Kelley University of Florida
==============================Original Headers============================== 6, 40 -- From vau-at-ufl.edu Mon Feb 9 17:12:33 2015 6, 40 -- Received: from smtp.ufl.edu (smtp-prod04.osg.ufl.edu [128.227.74.220]) 6, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t19NCWHo018835 6, 40 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Feb 2015 17:12:32 -0600 6, 40 -- X-UFL-GatorLink-Authenticated: authenticated as () with from 10.241.106.146 6, 40 -- Received: from UFEXCH-CASHT02.ad.ufl.edu ([10.241.106.146]) 6, 40 -- by smtp.ufl.edu (8.14.4/8.14.4/3.0.0) with ESMTP id t19NCVAc019774 6, 40 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 6, 40 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Feb 2015 18:12:32 -0500 6, 40 -- Received: from UFEXCH-MBXN03.ad.ufl.edu (10.253.18.73) by 6, 40 -- UFEXCH-CASHT02.ad.ufl.edu (10.241.106.146) with Microsoft SMTP Server (TLS) 6, 40 -- id 14.3.181.6; Mon, 9 Feb 2015 18:12:32 -0500 6, 40 -- Received: from UFEXCH-MBXN02.ad.ufl.edu ([fe80::4801:abc9:e123:b1bb]) by 6, 40 -- UFEXCH-MBXN03.ad.ufl.edu ([fe80::e09b:9163:ed13:4711%18]) with mapi id 6, 40 -- 14.03.0181.006; Mon, 9 Feb 2015 18:12:31 -0500 6, 40 -- From: "Kelley,Karen L" {vau-at-ufl.edu} 6, 40 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 6, 40 -- Subject: anti-catalase 1Ab for TEM 6, 40 -- Thread-Topic: anti-catalase 1Ab for TEM 6, 40 -- Thread-Index: AQHQRL3hebxG7gANkUGY2qGV92XAVA== 6, 40 -- Date: Mon, 9 Feb 2015 23:12:30 +0000 6, 40 -- Message-ID: {D0FEA918.FFCF%vau-at-ufl.edu} 6, 40 -- Accept-Language: en-US 6, 40 -- Content-Language: en-US 6, 40 -- X-MS-Has-Attach: 6, 40 -- X-MS-TNEF-Correlator: 6, 40 -- user-agent: Microsoft-MacOutlook/14.0.0.100825 6, 40 -- x-originating-ip: [10.36.9.36] 6, 40 -- Content-Type: text/plain; charset="us-ascii" 6, 40 -- Content-ID: {C1D377045D81BC4797555A963C45145C-at-ad.ufl.edu} 6, 40 -- MIME-Version: 1.0 6, 40 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.13.68,1.0.33,0.0.0000 6, 40 -- definitions=2015-02-09_04:2015-02-09,2015-02-09,1970-01-01 signatures=0 6, 40 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 6, 40 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 6, 40 -- engine=7.0.1-1402240000 definitions=main-1502090228 6, 40 -- X-Spam-Level: * 6, 40 -- X-UFL-Spam-Level: * 6, 40 -- Content-Transfer-Encoding: 8bit 6, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t19NCWHo018835 ==============================End of - Headers==============================
it may be that it is not the primary that is not working. Do you have positive controls with the secondaries? Catalase is usually so abundant and some molecules would always survive in my opinion, certainly in HM20 preps. Feel free to contact me or Peter van de Plas (email below) off-list if you would like some ideas on how to take this further.
Thank you and good luck,
Jan Leunissen Aurion
Aurion ImmunoGold Reagents Binnenhaven 5 6709 PD Wageningen The Netherlands
I looked up this antibody. It might be helpful if you can share the protocol you used for sample preparation and immunolabeling. Feel free to call or email. Thanks.
Hong Emory EM 404 712 8491 hyi-at-emory.edu
On 2/9/15 6:16 PM, "vau-at-ufl.edu" {vau-at-ufl.edu} wrote:
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==============================Original Headers============================== 11, 41 -- From hyi-at-emory.edu Mon Feb 9 21:41:30 2015 11, 41 -- Received: from ndb-mr3.cc.emory.edu (ndb-mr3.cc.emory.edu [170.140.53.253]) 11, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1A3fUMU029504 11, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 21:41:30 -0600 11, 41 -- Received: from e14edge2w.Emory.Edu (e14edge2w.eu.emory.edu [170.140.108.22] (may be forged)) 11, 41 -- by ndb-mr3.cc.emory.edu (8.13.8/8.13.8) with ESMTP id t1A3fSPD026892 11, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 22:41:29 -0500 11, 41 -- Received: from E14CH1N.Enterprise.emory.net (10.240.8.113) by 11, 41 -- e14edge2w.Emory.Edu (170.140.108.22) with Microsoft SMTP Server (TLS) id 11, 41 -- 14.3.224.2; Mon, 9 Feb 2015 22:41:28 -0500 11, 41 -- Received: from E14MBX11W.Enterprise.emory.net ([fe80::2cf2:45a4:a1cc:51f7]) by 11, 41 -- e14ch1n.Enterprise.emory.net ([::1]) with mapi id 14.03.0174.001; Mon, 9 Feb 11, 41 -- 2015 22:41:28 -0500 11, 41 -- From: "Yi, Hong" {hyi-at-emory.edu} 11, 41 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 41 -- Subject: Re: [Microscopy] anti-catalase 1Ab for TEM 11, 41 -- Thread-Topic: [Microscopy] anti-catalase 1Ab for TEM 11, 41 -- Thread-Index: AQHQRL55aOiDc8+gAEiLbAq4L7mFFJzpPW2A 11, 41 -- Date: Tue, 10 Feb 2015 03:41:28 +0000 11, 41 -- Message-ID: {D0FEE5AD.155F2%hyi-at-emory.edu} 11, 41 -- References: {201502092316.t19NGdf1021960-at-ns.microscopy.com} 11, 41 -- In-Reply-To: {201502092316.t19NGdf1021960-at-ns.microscopy.com} 11, 41 -- Accept-Language: en-US 11, 41 -- Content-Language: en-US 11, 41 -- X-MS-Has-Attach: 11, 41 -- X-MS-TNEF-Correlator: 11, 41 -- user-agent: Microsoft-MacOutlook/14.4.7.141117 11, 41 -- x-originating-ip: [71.236.2.128] 11, 41 -- Content-Type: text/plain; charset="us-ascii" 11, 41 -- Content-ID: {ED19DE58AAC0244BA26B6C69701DE399-at-Enterprise.emory.net} 11, 41 -- MIME-Version: 1.0 11, 41 -- X-Emory-MailScanner-Information: Please contact the ISP for more information 11, 41 -- X-Emory-MailScanner-ID: t1A3fSPD026892 11, 41 -- X-Emory-MailScanner: Found to be clean 11, 41 -- X-Emory-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 11, 41 -- score=-0.01, required 8, autolearn=disabled, 11, 41 -- T_RP_MATCHES_RCVD -0.01) 11, 41 -- X-Emory-MailScanner-From: hyi-at-emory.edu 11, 41 -- X-Spam-Status: No 11, 41 -- Content-Transfer-Encoding: 8bit 11, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1A3fUMU029504 ==============================End of - Headers==============================
The Southeastern Microscopy Society would like to invite you to join us for the Society's 51st Annual meeting to be held May 20-22, 2015. The meeting will be held at the Courtyard Marriott hotel in downtown Decatur, GA located at 130 Clairemont Avenue. Further, I would encourage you and your students to consider submitting an abstract for a poster or platform presentation at the meeting.
All information regarding the meeting can be found on the SEMS website. Please visit http://southeasternmicroscopy.org/2015-meeting/ for registration and the Call for Papers. All abstracts should be submitted by April 17. Students should be encouraged to present their research through the Ruska competition. Ruska participants receive meeting registration, housing at the meeting hotel (applicants must be willing to share a room with other students), a banquet ticket, and a certificate of participation. The first place Ruska Award winner will receive the Ruska Award plaque and $300.00 at the SEMS banquet. This is great opportunity for students!
The Invited speakers will be Dale Newbury, with NIST; Lucille Giannuzzi, with L.A. Giannuzzi & Associates; and Peter Kner, with the University of Georgia. In addition, Robert Simmons from Georgia State University has agreed to talk about Microscopy and Art for the banquet.
The meeting will start Wednesday, May 20, with workshops and technical talks. Wednesday evening we will have the Mixer and poster session starting at 6 PM. Thursday's schedule contains invited and submitted presentations and opportunities to visit our vendors. The Banquet will be Thursday evening. Plan to attend the Business Breakfast on Friday morning and finish off attending some final presentations, with the meeting ending at noon.
Hotel rooms will cost $129 + tax, and reservations can be made at http://cwp.marriott.com/atldc/sems or by calling the hotel at 404-371-0204 at asking for the SEMS special rate. Reservation must be made by April 28 to receive this rate.
Russ Goddard SEMS President
Amanda Lawrence Outreach Coordinator/Research Associate Institute for Imaging and Analytical Technologies Mississippi State University
==============================Original Headers============================== 11, 31 -- From ALawrence-at-i2at.msstate.edu Tue Feb 10 13:24:45 2015 11, 31 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1AJOjo9000614 11, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2015 13:24:45 -0600 11, 31 -- Received: from mail05.ad.msstate.edu (mail05.ad.msstate.edu [130.18.230.64]) 11, 31 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id t1AJOixd008628 11, 31 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 11, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2015 13:24:45 -0600 11, 31 -- X-Sender: {} 11, 31 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by 11, 31 -- mail05.ad.msstate.edu (2002:8212:e640::8212:e640) with Microsoft SMTP Server 11, 31 -- (TLS) id 15.0.913.22; Tue, 10 Feb 2015 13:24:41 -0600 11, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 11, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 11, 31 -- 15.00.0913.011; Tue, 10 Feb 2015 13:24:41 -0600 11, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 11, 31 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 11, 31 -- Subject: Southeastern Microscopy Society (SEMS) Annual Meeting 11, 31 -- Thread-Topic: Southeastern Microscopy Society (SEMS) Annual Meeting 11, 31 -- Thread-Index: AdBFZlZFVh92mi/UQeC30qvCK0LQLA== 11, 31 -- Date: Tue, 10 Feb 2015 19:24:40 +0000 11, 31 -- Message-ID: {ba551c2f7b7c454391c2540a94b9192c-at-mail02.ad.msstate.edu} 11, 31 -- Accept-Language: en-US 11, 31 -- Content-Language: en-US 11, 31 -- X-MS-Has-Attach: 11, 31 -- X-MS-TNEF-Correlator: 11, 31 -- x-originating-ip: [130.18.230.93] 11, 31 -- Content-Type: text/plain; charset="us-ascii" 11, 31 -- MIME-Version: 1.0 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1AJOjo9000614 ==============================End of - Headers==============================
We are submitting a NSF proposal for new equipment and came across a requirement for a data management plan. At this moment my facility users are responsible for their own data. I'm interested to find out what others are using for their data management plans. We are a "Google" university (Mail/Calendar etc.) - would storing data on their Drive folder be adequate?
Thanks in advance!
Owen
Owen Mills Michigan Tech University Houghton, MI
==============================Original Headers============================== 5, 34 -- From opmills-at-mtu.edu Tue Feb 10 13:53:35 2015 5, 34 -- Received: from mail-ie0-f178.google.com (mail-ie0-f178.google.com [209.85.223.178]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1AJrZ1l024383 5, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2015 13:53:35 -0600 5, 34 -- Received: by iebtr6 with SMTP id tr6so27420226ieb.4 5, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2015 11:53:35 -0800 (PST) 5, 34 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 34 -- d=1e100.net; s=20130820; 5, 34 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 5, 34 -- :subject:content-type:content-transfer-encoding; 5, 34 -- bh=HnimjtjZhVRMLF79perpaxDSN40i0rFTOVsqA/8JmdI=; 5, 34 -- b=e3PqhNDPGdiuaPLx9dw7QBc4LucCNhWFXgwpuZL7bMCDqMNfd7GP5q2PUBwfDNmtyL 5, 34 -- rsA+KMADaRvKXGXpLoZPpF6pFrDZ2lLdEmCakGD9MNH3aZ7gu7h5Aahx0dZRLpQbNVq4 5, 34 -- aC0Lsqq9RIvfPGMRayHRmQrjEcgRTjdxDyVshU/bPpVfiThwZ/vdaGSF6ZKiW3s4BI6A 5, 34 -- JXPDts7t1j1iVTlEG2kj31rFsrFkyR0/R8SNl0w8pZ1GLPueHDWOJCi84C9Evu2hffML 5, 34 -- 9/aQbpUgouapf79nw8bJam+BMzJyUR1VqnPu7H3q1+qWKcHHO2VIp9vVBIS/s4UAcWm3 5, 34 -- Lx8g== 5, 34 -- X-Gm-Message-State: ALoCoQlDX7DWHDm8NhRAPdbedu+XdtTCfZKc+cNYnt699hNb0O5h73RirBXWFEqhVylKSw2/JcRw 5, 34 -- X-Received: by 10.43.140.68 with SMTP id iz4mr27964599icc.77.1423598013948; 5, 34 -- Tue, 10 Feb 2015 11:53:33 -0800 (PST) 5, 34 -- Received: from e12-0626-fac.coe.ad.mtu.edu (e12-0626-fac.coe.ad.mtu.edu. [141.219.30.140]) 5, 34 -- by mx.google.com with ESMTPSA id m77sm9196236iom.38.2015.02.10.11.53.32 5, 34 -- for {Microscopy-at-microscopy.com} 5, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 5, 34 -- Tue, 10 Feb 2015 11:53:33 -0800 (PST) 5, 34 -- Message-ID: {54DA627E.4090400-at-mtu.edu} 5, 34 -- Date: Tue, 10 Feb 2015 14:56:46 -0500 5, 34 -- From: Owen Mills {opmills-at-mtu.edu} 5, 34 -- User-Agent: Postbox 3.0.11 (Macintosh/20140602) 5, 34 -- MIME-Version: 1.0 5, 34 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 34 -- Subject: NSF data management plans 5, 34 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 34 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: MICROSCOPY SOCIETY OF NORTHEASTERN OHIO
Title-Subject: [Filtered] MSNO 2015 Meeting
Message: I'd like to invite you to join MSNO Winter Meeting on Wednesday, March 4th, 2015, 3:00 Â8:30 p.m. at Cleveland Museum of Natural History.
Lillian A. Kuri from Cleveland Foundation will give a talk on "ClevelandÂs Greater University Circle Initiative" and John Hemsath, a Director of Theater Operations will give a talk on "The History of Playhouse Square".
This meeting will give us two successful demonstrations on how collaboration could make a wonderful impact and we could also make such a significant impact to our society too.
Pre-registration and more detail could be found at http://www.msneo.org/2015-winter-meeting.html
We hope to see you at the meeting.
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Email: kpseverin-at-alaska.edu Name: Ken Severin
Organization: University of Alaska Fairbanks
Title-Subject: [Filtered] HV cable for Ladd sputter coater
Message: The HV cable on my old Ladd/Polaron (also sold as ISI and a few other brands) went up in smoke. If someone has a spare or source (or a simple sputter coater that is no longer in use) please contact me off line and we'll work out a deal.
Thanks much!
Ken Severin University of Alaska Fairbanks kpseverin-at-alaska.edu
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Email: bob.price-at-uscmed.sc.edu Name: Bob Price
Organization: University of South Carolina
Title-Subject: [Filtered] Workshop on Basic Confocal Microscopy
Message: During the week of June 15-19 the University of South Carolina Instrumentation Resource Facility will again host the "Basic Confocal Microscopy Workshop." Workshop material is directed towards beginning and intermediate users of confocal microscopes and involves a series of lectures (specimen preparation, labeling strategies, proper set-up of instrument operating parameters, proper handling of 2D and 3D confocal images in programs such as Adobe Photoshop and AMIRA), hands on specimen preparation, and time on a number of different point scanning and spinning disk confocal microscopes.
This is a hands on workshop where participants will stain cells and tissues with multiple labels, collect images, and participate in image analysis exercises using Photoshop and AMIRA.
Faculty will include Dr. Jay Jerome of Vanderbilt University, Dr. Ralph Albrecht of the University of Wisconsin Madison, Dr. John Mackenzie of North Carolina State University, Dr. Tom Trusk of the Medical University of South Carolina, and Dr. Bob Price of the University of South Carolina. Several companies will also have equipment and applications experts on hand to assist with instrumentation and questions about confocal microscopy research protocols.
Cost for the entire week is $350 which covers meals and supplies for the workshop. Please register soon as the workshop has filled the past several years.
For further information please see: http://irf.med.sc.edu/
For questions and registration contact Anna McNeal (anna.mcneal-at-uscmed.sc.edu or Bob Price (bob.price-at-uscmed.sc.edu
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On Feb 9, 2015, at 10:30 AM, frank_karl-at-ardl.com wrote:
} Hello Everyone, } I'm looking for suggestions about a beam sensitive sample. } } I'm cutting thin sections with a cryomicrotome of HIPS, High Impact } Polystyrene, that is toughened with polybutadiene. I'm cutting at } -100 C because of the low TG of the polybutadiene and staining with } Os. } My thin sections are very beam sensitive, too sensitive to focus } well and get a good image. I'm imaging at 80KV with 8800X } magnification. If I spread out my beam to reduce beam damage I } can't see my sample to focus and any pictures I take look crappy. } } Any suggestions? } } I've been thinking about trying carbon coated grids to help act as a } heat sink as well as going to a larger TEM spot setting. I have read } that lower KV can make the damage worse because each electron } resides in the thin section longer and produced more heat. Is this } a tried and true observation? } } Thanks!!! } } Frank } } This email and any of its attachments may contain confidential } information intended only for the use of the addressee(s). If the } reader of this email is not the intended recipient or the employee } or agent responsible for delivering it to the intended recipient, } you are hereby notified that any dissemination or copying of this } email is strictly prohibited. If you have received this email in } error, please notify us by return email at info-at-ardl.com, } permanently delete the email, and destroy any printouts. If this } email contains test data and/or draft reports, you are hereby } notified that only a signed original test report will contain } official results, a copy of which resides in the project folder } located at ARDL, Inc. Thank you. Akron Rubber Development } Laboratory, Inc. } } Dear Frank, Since you have cryosectioning capability, can I assume you have cryomicroscopy capability? If so, do you have a low-dose capability? In addition to carbon-coating, you could put a few gold nanoparticles onto the specimen, use the low-dose settings to find an area a micrometer or so away from the imaging area, focus pretty quickly on the gold beads--easier with FEG or very coherent LaB6--then take the image. You could even burn a small hole at the focussing position (assuming that the specimen can survive that) without having the beam overlap the imaging position. If you do not have low-dose capability, you might try using an image shift setting to get to a focussing area-- calibrate with a cross grating--and return the shift to its original setting for imaging. All this shifting should be done with a pre- specimen shutter engaged between shifts to avoid unexpected exposure of the specimen. Good luck. Yours, Bill
==============================Original Headers============================== 6, 32 -- From wtivol-at-sbcglobal.net Wed Feb 11 20:46:34 2015 6, 32 -- Received: from nm5-vm4.access.bullet.mail.gq1.yahoo.com (nm5-vm4.access.bullet.mail.gq1.yahoo.com [216.39.63.93]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1C2kYQJ024088 6, 32 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2015 20:46:34 -0600 6, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1423709194; bh=ANalGMYfWm6XiJl1uxYSFAp+6o/TDeIS1aVl4jtfvEg=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=ijGr3i0bOs/+0l4Rt1vv4Tp0R2TI/3vQOwgZAY2kPkI/pt4taPMEsgy6A/35FvE9swbCswyOjV14SQJpIKGowMR8KeHaNT/YpfAIGXfKUcBQzswV+AXTYJ3AkRToYv6/5HGLUhQhWnssuWRzFS2FgGhwu3M/0vo+QckJyDIllt0SRV5lt5G6t+mybp8x8aEYGznWttfKoPj9QVQwylae+L+BeoN9YV2LHz2ckpNue1yrGsMwGL229qELV9JP1CAny+36SuTrm/j4abyGay+hBldoj+NjaW3JiK5zOHHIcBBqoKODQ99sMX82pseZB7LVMdT+DU+CEGUA59e7WEfbzA== 6, 32 -- Received: from [216.39.60.170] by nm5.access.bullet.mail.gq1.yahoo.com with NNFMP; 12 Feb 2015 02:46:34 -0000 6, 32 -- Received: from [67.195.23.144] by tm6.access.bullet.mail.gq1.yahoo.com with NNFMP; 12 Feb 2015 02:46:34 -0000 6, 32 -- Received: from [127.0.0.1] by smtp116.sbc.mail.gq1.yahoo.com with NNFMP; 12 Feb 2015 02:46:34 -0000 6, 32 -- X-Yahoo-Newman-Id: 79688.63392.bm-at-smtp116.sbc.mail.gq1.yahoo.com 6, 32 -- X-Yahoo-Newman-Property: ymail-3 6, 32 -- X-YMail-OSG: 5hWdtEUVM1n8w3XKR2m3i8j.yR.n2xvTFFJTMLjogU.HxIC 6, 32 -- A4fwnhGP3P2fQ7qodSqVCUmP.bVnI9iqWkroyeaORc9anoa00KHetgSq2ISp 6, 32 -- TuQcAQvJGQ6ijAQGQJopvYwkcDn88YIhgfdd_Ptq5ldCB7Oq2IolVstIrGnc 6, 32 -- AHaGYhwfeHX8u55SF8A2.751_vT6Lw2SKQtz_h5sjd40wEZqxJDPdppEXObj 6, 32 -- j826fGUmBcZ65nRqlGzJ03zyVqrn4trGd9ZKLeT0n9trYJX5pEvqCgFUuKJS 6, 32 -- vjYoxIIDp2ZS07DKeS.0aQ93q0mt1y6iv7NmDG6IOZlEic13c93.eC.VnMRB 6, 32 -- RY8QMSLJ1PHyyX5svejJprItCHMmKx5K78.MfPWyojDFx3ODXA.C3SfN2LD. 6, 32 -- AYu3u68hz.L4273he2O8NhEhnc6cJPKc8W1mqRcC85RCYBkx7lm0KSj.g668 6, 32 -- iJlNTdDq9WvEgJ8gg.TuXbEkwb6fQWslQ9HdxkuyrKPhbIzpHW3VnxIiJcW. 6, 32 -- csnZUnBxAXXvdDoU.SvTssYI3hc7DRS4.v1cr 6, 32 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 32 -- Message-Id: {C445CC56-20C1-44E8-A4F6-6E41F3F766C0-at-sbcglobal.net} 6, 32 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 32 -- To: Frank Karl {frank_karl-at-ardl.com} , microscopy-at-microscopy.com 6, 32 -- In-Reply-To: {201502091830.t19IULUS002451-at-ns.microscopy.com} 6, 32 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 32 -- Content-Transfer-Encoding: 7bit 6, 32 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 32 -- Subject: Re: [Microscopy] TEM and HIPS 6, 32 -- Date: Wed, 11 Feb 2015 18:46:30 -0800 6, 32 -- References: {201502091830.t19IULUS002451-at-ns.microscopy.com} 6, 32 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
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Email: rms-at-angstrom.us Name: Bob Sommerville
Organization: Angstrom Scientific Inc.
Title-Subject: [Filtered] UCLA Workshop on Imaging Kinetics at the Atomic Level
Message: Wednesday, February 18th at the UCLA California NanoSystems Institute 570 Westwood Plaza, Building 114 Los Angeles, CA 90095 9:30am: Invited Talks (Executive Conference Room) 1:30pm: Live Demonstrations (Room B145) Please RSVP to: rms-at-angstrom.us ** Talks will be followed by lunch and then a live demonstration of the capabilities of the DENSsolutions heating holder on the EICNÂs FEI Titan Krios. We encourage participants to bring samples. Invited talk by Dr. Paul Voyles Professor, Materials Science and Engineering, University of Wisconsin-Madison Atomic Rearrangements in Glass-Forming Metal Alloys Melted Inside the STEM Atomic rearrangements in the liquid state are fundamental to transformations of materials including crystallization and the glass transition, but they occupy a difficult to access regime of time- and length-scale. For glass-forming liquids, the relaxation time, which is the characteristic time for atoms to switch neighbors, ranges from microseconds or less in the high-temperature equilibrium liquid to hundreds of seconds in the deeply supercooled liquid near the glass transition temperature, Tg. The length scale is likely to be on the nanometer to sub-nanometer scale, but is largely unknown. We have used time-resolved coherent electron nanodiffraction in the STEM to measure for Pd40Ni40P20 alloy in the supercooled liquid from Tg to Tg + 40 K with 2 nm spatial resolution. This new experimental technique, called electron correlation microscopy, is the electron equivalent of x-ray photon correlation spectroscopy, but at higher spatial resolution. It is made possible by recent advances in electron microscope instrumentation, including highly coherent electron sources, high stability heating holders, and fast electron detectors. Additional presentations: MaterialÂs kinetics at the atomic level Eric Kievit, Director of Sales, DENSsolutions Revealing sintering behavior of SiC nanoparticles by in-situ heating HRTEM Dr. Lianyi Chen, Department of Mechanical and Aerospace Engineering, UCLA DENSsolutions Provides Sample Management Solutions for in-Situ Electron Microscopy Angstrom Scientific Inc. is a US Distributor providing characterization solutions to the nanotech marketplace. Angstrom supports DENS throughout the US as well as other principals such as Kleindiek and Hitachi for Tabletop TM 3030 SEM's and for key products and accessories related to TEM, SEM and Dual Beam markets
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Email: ech-at-uvic.ca Name: Elaine Humphrey
Organization: University of Victoria
Title-Subject: [Filtered] M&M 2015 Family Affair ProjectMICRO and Foldscopes
Message: Dear All Last year in Hartford at the M&M the Solve the Mystery was based on the story: Secret Agent, James B Atom, wrote a message for Headquarters. It was written on a FIB and could only be read on an sem (too small for a light microscope. It was cut into four pieces and your task should you choose to accept it, is to take your piece of the message to the Q's lab (Exhibit Hall where we have some vendors to work with you) to read it. Once deciphered you should take it to Headquarters (the Outreach Booth) where it will be put together.
The message read "Never trust an Atom, they make up everything"
Everyone who attended seemed to particularly enjoy this bit, so I was thinking of doing it again but with a different message. Any ideas?
Also I put our name down for some Foldscopes (origami microscopes) which have just arrived and I intent to bring them to the Family Affair at M&M. I hope to have one at the Outreach booth too. http://www.foldscope.com/
The Project Micro workshop since we will be in Portland, will be the ever popular "The Private Eye workshop" http://the-private-eye.com/index.html This is one of the best outreach workshops to help Elementary School Teachers get sucked into Science even though they may be intimidated by Science. They use a 5x loupe.
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Išm sure this question has been asked many times before, but I wanted to see if people might share their systems for keeping track of TEM samples. In grad school it wasnšt too bad, since I was focused mainly on my own samples, but now Išve been deluged with samples from many different projects.
Išm considering using Evernote in combination with a naming and tracking scheme, but Išm open to other options as well.
Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
Hi Steven, X-from my experience, having good tracking, a naming method and physical storage for samples is key. I would suggest going beyond a simple text file, as this becomes unwieldy quickly. A database can be as simple as a spreadsheet, with columns for all the parameters of interest and links to image storage locations. A spreadsheet can be filtered and searched and doesn't require any programming - unless you need to do a real database for PNNL tracking or billing. Set viewing and editing permissions so that only those you trust to enter data properly can make changes. A physical organizer for grid boxes, with names that relate to your database for getting at them later if desired, is really helpful. Good luck! Regards, Larry Scipioni ZS Genetics
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: Thursday, February 12, 2015 6:57 PM To: LES-at-ZSGENETICS.COM
Hello everyone,
Išm sure this question has been asked many times before, but I wanted to see if people might share their systems for keeping track of TEM samples. In grad school it wasnšt too bad, since I was focused mainly on my own samples, but now Išve been deluged with samples from many different projects.
Išm considering using Evernote in combination with a naming and tracking scheme, but Išm open to other options as well.
Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
We give each project a unique project number, which is incorporated into sample labels, processing records and image records, as well as a project database (currently Filemaker pro) so every step can be traced back to the project. Ours is prefixed by an identifying code, followed by the financial year, followed by the project number - ie, EMLP 14-15.037
The database includes client name, department (or company), order number, brief description of work, financial details etc etc. Each project has a processing protocol and job summary that is filed in a folder headed with the corresponding project number.
This way, as long as you've got the project number, you can refer back to any sample.
We also give each image a unique image number for the same reason.
All the best,
Natalie
Miss Natalie Allcock CBS Electron Microscopy Facility University of Leicester
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: 12 February 2015 23:58 To: Allcock, Natalie S.
Hello everyone,
Išm sure this question has been asked many times before, but I wanted to see if people might share their systems for keeping track of TEM samples. In grad school it wasnšt too bad, since I was focused mainly on my own samples, but now Išve been deluged with samples from many different projects.
Išm considering using Evernote in combination with a naming and tracking scheme, but Išm open to other options as well.
Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
We've got an old Model 8000 IMIX PC EDS system lying around made by Princeton Gamma Tech and we were wondering if anyone might need it for spare parts, or whatever. Let us know.
ERiC Jay Miller Microscopy & Imaging Specialist Electron Probe Instrumentation Center
Northwestern University Mail: 2036 Cook Hall Office: 1152 Cook Hall 2220 Campus Drive Evanston, IL 60208-3108
ph: (847) 467-0789
http://www.nuance.northwestern.edu
==============================Original Headers============================== 9, 56 -- From eric-miller-at-northwestern.edu Fri Feb 13 17:02:19 2015 9, 56 -- Received: from evcspsym1.ads.northwestern.edu (evcspsym1.ads.northwestern.edu [129.105.238.5]) 9, 56 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1DN2Joi027678 9, 56 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Feb 2015 17:02:19 -0600 9, 56 -- X-AuditID: 8169ee05-f797b6d0000014e3-fd-54de827b2d6e 9, 56 -- Received: from evcspprf3.ads.northwestern.edu (evcspprf3.ads.northwestern.edu [165.124.82.242]) 9, 56 -- by evcspsym1.ads.northwestern.edu (Symantec Messaging Gateway) with SMTP id C5.6D.05347.B728ED45; Fri, 13 Feb 2015 17:02:19 -0600 (CST) 9, 56 -- Received: from pps.filterd (evcspprf3.ads.northwestern.edu [127.0.0.1]) 9, 56 -- by evcspprf3.ads.northwestern.edu (8.14.7/8.14.7) with SMTP id t1DM1hnd025981 9, 56 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Feb 2015 15:02:14 -0800 9, 56 -- Received: from evcspcas3.ads.northwestern.edu (evcspcas3.ads.northwestern.edu [129.105.68.135]) 9, 56 -- by evcspprf3.ads.northwestern.edu with ESMTP id 1sh0xq0vvq-1 9, 56 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Feb 2015 15:02:14 -0800 9, 56 -- Received: from EVCSPMBX3.ads.northwestern.edu ([169.254.3.67]) by 9, 56 -- EVCSPCAS3.ads.northwestern.edu ([129.105.68.135]) with mapi id 9, 56 -- 14.03.0158.001; Fri, 13 Feb 2015 17:02:18 -0600 9, 56 -- From: Eric Jay Miller {eric-miller-at-northwestern.edu} 9, 56 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 56 -- Subject: IMIX-PC EDS by PGT 9, 56 -- Thread-Topic: IMIX-PC EDS by PGT 9, 56 -- Thread-Index: AdBH4MQ2XraouV8ATxKz66/43CQ+yg== 9, 56 -- Date: Fri, 13 Feb 2015 23:02:17 +0000 9, 56 -- Message-ID: {1A377C4705E2B14C8D55336F80E66047018EA533-at-evcspmbx3.ads.northwestern.edu} 9, 56 -- Accept-Language: en-US 9, 56 -- Content-Language: en-US 9, 56 -- X-MS-Has-Attach: 9, 56 -- X-MS-TNEF-Correlator: 9, 56 -- x-originating-ip: [165.124.43.167] 9, 56 -- Content-Type: text/plain; charset="us-ascii" 9, 56 -- MIME-Version: 1.0 9, 56 -- X-Proofpoint-Virus-Version: vendor=nai engine=5400 definitions=5800 signatures=585085 9, 56 -- X-Proofpoint-Spam-Details: rule=audit_notspam policy=audit score=0 spamscore=0 suspectscore=0 9, 56 -- phishscore=0 adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx 9, 56 -- scancount=1 engine=7.0.1-1402240000 definitions=main-1409170064 9, 56 -- X-Brightmail-Tracker: H4sIAAAAAAAAA02TbUxTVxjH89y+eEEuXgqUx8rI1sxpfEE0W7LhavywD7DNZM4Sk22JVHql 9, 56 -- HbWw3oLCNgSCQ5DNEjq0ZdrCkBXXrBlhxgBGVrepEOaI8XXYieDGS3GbEIWtuJ3bF+iXm+f+ 9, 56 -- /uf5n/85Tw4tknXFKGi90cyZjBqDUhorPv3R2482fljlU2c0NyS9HLhYsWw7ZP19dIp6C96J 9, 56 -- fVXLGfQlnGnTttxYXe/3N6miAdFBl8MuqYBpqg5oGtkX0XJcVgcxpJTjLz6PtA5iaRk7ATg/ 9, 56 -- 66BCP3cAJy8dDisjgA1XXeLQTxegbfCWROiXEquFoWapYJvEbsUfO1UCTmRTcXbuuiSEn8OB 9, 56 -- CbGAk9h0tJ61gFCL2dVYc+3sMqFm2Bw83GQJOgJJ9KTfTQm1iE3BO2MOKpQ0AVube0WR1E+7 9, 56 -- R6Shwyhx+EZuaPkGdPY8kobq9djeMiUK2SfgFduYONSag+P+r4InQXYKsH3eCRZIsUdtZ4/y 9, 56 -- skd52aO8nCA+A2lcSR5fxJfu35yu0fLpxkKTWXeA44U5pXPa4k4go6rUP5Scg2ONG73A0qCM 9, 56 -- Yyxv3lXLJJoS0uaFN2hKmcx4PvapZfF7C7WlOg2v22MqNnC8MonxVRDMLOK9xYYCpYJRVxKa 9, 56 -- uEiN3AHewJnJnl5AWkTatu0U2rSa0jLOVBgy88IqWqxMYeZjhtQyNl9j5go4rogzRVQ1TSuR 9, 56 -- uSfsl2Di8rmD+/QGc0QmfU90RGGjlWCYZ5j3M4kgjxai81B0jBey6DgSyiCkZvgizX5enx/2 9, 56 -- TWSKhR3jIjTouZIpEaAsApf8+iGPdtZcsFEysbHQyClSGFowZYWVumLjYl6FnEm7R+54RZQg 9, 56 -- WCtSma2jhCdH8SX3yLOahACQOSUy/wWzkVe3FFjGvFJO4PIwDOZFpiZ4bWG2ZLili/iwQxT6 9, 56 -- na0MftZWG49z/vMrcGzBn4wnZqvk6GuoewE/P+Veg23tThW6rsxlY8/lgBpdE905WF/bthvn 9, 56 -- hy7sRvvMxX342+1PCvDU0y8MONPxoBzdoz2VgH3DjipA67HAEcC/OmtrAS/b/iFfz8JoPeD9 9, 56 -- 6REr4MzjqzbA4aOftgDeGj7dSnjH8T7A8c7GnwB/6JwfALR0tw4C+i+ND8IkGRhFBtZw4ldh 9, 56 -- YGaNOXpgboHGRWh4YGcEKIvApRtQVEDmyco8+jVr4Jve7363vrfzy1RV+Y7tetX97kBNRj99 9, 56 -- 6M9nDdWqdzPiV16/kZ2dNfet+4M1ezyvp/XrWyTXmtZVa9cONw3Yfi5bmzP9R3WjRT3uul3v 9, 56 -- Gaf7fY8fDm5K7ct1fJ2yYbnD0td8chV2bPn3+ZurXzqfuV5+6FxZ+667ux4ccXUpxbxOs3md 9, 56 -- yMRr/gcYypyJRwUAAA== 9, 56 -- Content-Transfer-Encoding: 8bit 9, 56 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1DN2Joi027678 ==============================End of - Headers==============================
I want to thanks all the advice I received about cutting cryothin sections of HIPS. The carbon coated grids worked great as did increasing the KV to 100. I stained my thin sections on the grids with OsO4 vapor for two hours and that made the phase really "POP!"
Thanks again. Now I'm hip about HIPS.
Frank
PS, I just really want more out of office remarks, it's like cowbell, how can you have too much!!!
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 9, 28 -- From frank_karl-at-ardl.com Wed Feb 18 07:12:05 2015 9, 28 -- Received: from cal1-mh779.smtproutes.com (cal1-mh779.smtproutes.com [208.70.91.145]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1IDC4kR016686 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 18 Feb 2015 07:12:04 -0600 9, 28 -- X-Katharion-ID: 1424265096.26986.cal1-mh779 9, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by 9, 28 -- cal1-mh779.smtproutes.com [(192.69.16.145)] with ESMTP via TCP 9, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 18 Feb 2015 13:11:36 +0000 9, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 9, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Wed, 18 9, 28 -- Feb 2015 08:11:36 -0500 9, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 9, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 9, 28 -- {microscopy-at-microscopy.com} 9, 28 -- Date: Wed, 18 Feb 2015 08:11:35 -0500 9, 28 -- Subject: Thanks !!! 9, 28 -- Thread-Topic: Thanks !!! 9, 28 -- Thread-Index: AdBLfGutJoPPrJaKSYq7WxmAjNbh7Q== 9, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C88CAFE59-at-exchange2k7.ad.ardl.com} 9, 28 -- Accept-Language: en-US 9, 28 -- Content-Language: en-US 9, 28 -- X-MS-Has-Attach: 9, 28 -- X-MS-TNEF-Correlator: 9, 28 -- acceptlanguage: en-US 9, 28 -- Content-Type: text/plain; charset="us-ascii" 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1IDC4kR016686 ==============================End of - Headers==============================
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Email: gary-at-gaugler.com Name: Dr. Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] LEO 1455VP LaB6
Message: Has anyone with a LEO/Zeiss 1455VP LaB6 SEM compared the Wehnelt aperture sizes? They are 1mm and 500um. Zeiss seems to have settled on the 500um Wehnelt aperture size for LaB6 and W emitters.
Is there any feedback from users about which aperture size produces the best results?
TIA, gary g.
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Email: connellyps-at-mail.nih.gov Name: Pat Connelly
Organization: NIH/National Heart, Lung and Blood Institute
Title-Subject: [Filtered] Position open at NIH
Message: There is an opening in the National Heart, Lung and Blood Institute in Bethesda, MD for an "Electron Microscopy Senior Scientist and/or Core Director".
The posting is listed on the MSA Site [Resources} Placement Office/Job Openings] as Job ID 22114004 with a full detailed description and application directions.
The job requirements state that applicants must have a PhD. (or equivalent) and be an experienced scientist with significant experience in many aspects of electron microscopy and a record of independent scientific productivity as evidenced by citable publications. Experience with correlative light/EM imaging, novel methods development, digital image processing and analysis, cryo-EM, and 3D EM methods are highly desirable. Excellent interpersonal skills are a requirement.
I believe that the same posting is also on the ASCB Job Board.
This message would not go through the List Server as I usually post with plain text.
Pat Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491 connellyps-at-mail.nih.gov
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
Does anyone have a schematic diagram of the electronics in the Emitech K-450 carbon coater? This is NOT the K-450X which is a later version. It appears that a part failed somewhere between the pirani gauge and the meter. Having the electrical diagram just might help with tracking it down.
Tom Kremer IPS Testing 3211 E. Capitol Drive Appleton, WI 54911 920-749-3040 ext.121 tkremer-at-ipstesting.com
==============================Original Headers============================== 4, 42 -- From tkremer-at-ipstesting.com Fri Feb 20 14:08:03 2015 4, 42 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1on0061.outbound.protection.outlook.com [157.56.110.61]) 4, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1KK80EM004662 4, 42 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2015 14:08:02 -0600 4, 42 -- Received: from DM2PR0701MB1020.namprd07.prod.outlook.com (25.160.24.150) by 4, 42 -- DM2PR0701MB1018.namprd07.prod.outlook.com (25.160.24.148) with Microsoft SMTP 4, 42 -- Server (TLS) id 15.1.93.16; Fri, 20 Feb 2015 20:07:52 +0000 4, 42 -- Received: from DM2PR0701MB1020.namprd07.prod.outlook.com ([25.160.24.150]) by 4, 42 -- DM2PR0701MB1020.namprd07.prod.outlook.com ([25.160.24.150]) with mapi id 4, 42 -- 15.01.0093.004; Fri, 20 Feb 2015 20:07:52 +0000 4, 42 -- From: Tom Kremer {tkremer-at-ipstesting.com} 4, 42 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 42 -- Subject: Emitech K-450 4, 42 -- Thread-Topic: Emitech K-450 4, 42 -- Thread-Index: AdBNR71/nTN/CqK/T+qaTvJLSTRIug== 4, 42 -- Date: Fri, 20 Feb 2015 20:07:51 +0000 4, 42 -- Message-ID: {DM2PR0701MB1020500E2FE271983249312BDB2A0-at-DM2PR0701MB1020.namprd07.prod.outlook.com} 4, 42 -- Accept-Language: en-US 4, 42 -- Content-Language: en-US 4, 42 -- X-MS-Has-Attach: 4, 42 -- X-MS-TNEF-Correlator: 4, 42 -- x-originating-ip: [96.11.14.178] 4, 42 -- authentication-results: spf=none (sender IP is ) 4, 42 -- smtp.mailfrom=tkremer-at-ipstesting.com; 4, 42 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID:;SRVR:DM2PR0701MB1018; 4, 42 -- x-microsoft-antispam-prvs: {DM2PR0701MB101823EC9F4CB263BE3DE3D5B52A0-at-DM2PR0701MB1018.namprd07.prod.outlook.com} 4, 42 -- x-exchange-antispam-report-test: UriScan:; 4, 42 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:;SRVR:DM2PR0701MB1018; 4, 42 -- x-forefront-prvs: 0493852DA9 4, 42 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(189002)(199003)(38414003)(450100001)(62966003)(76576001)(229853001)(86362001)(77156002)(102836002)(74316001)(66066001)(2351001)(2501002)(110136001)(68736005)(64706001)(107886001)(40100003)(106356001)(105586002)(99286002)(92566002)(50986999)(87936001)(33656002)(19580395003)(19580405001)(101416001)(2656002)(46102003)(122556002)(2900100001)(97736003)(54356999);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR0701MB1018;H:DM2PR0701MB1020.namprd07.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 4, 42 -- received-spf: None (protection.outlook.com: ipstesting.com does not designate 4, 42 -- permitted sender hosts) 4, 42 -- Content-Type: text/plain; charset="us-ascii" 4, 42 -- MIME-Version: 1.0 4, 42 -- X-OriginatorOrg: ipstesting.com 4, 42 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 20 Feb 2015 20:07:51.5606 4, 42 -- (UTC) 4, 42 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 4, 42 -- X-MS-Exchange-CrossTenant-id: fcd656df-08de-421e-a3c5-e640523dfad4 4, 42 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR0701MB1018 4, 42 -- Content-Transfer-Encoding: 8bit 4, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1KK80EM004662 ==============================End of - Headers==============================
At the University of Rochester Medical Center's Pathology Department in Rochester, NY we are looking to replace our retiring Kidney EM Clinical Laboratory Specialist..
Please contact Karen Bentley if you have any questions about this clinical position or you can go to the University of Rochester Medical Center's website and at the bottom of the page listed under URMC Information is the "Job Opportunities" link which will lead you to the jobs, type in # 185704.
This position requires sufficient knowledge to run the day-to-day operations of both the Transmission Electron Microscope (TEM) and Immunofluorescent (IF) laboratories, under supervision of the Medical Director of Renal EM and Autopsy who is responsible for those laboratories. Required skills, which can be acquired during the initial on the job training, include knowledge of normal and abnormal ultrastructural and immunofluorescence morphology.
Bachelor's degree in Clinical Laboratory Science and one year of experience; or an equivalent combination of education, experience or certification as required for New York State licensure. Experience in field of electron microscopy, renal pathology strongly preferred, but on the job training will be provided to the right candidate.
Karen Bentley, M.S. Director Electron Microscope Research Core Pathology & Laboratory Medicine University of Rochester Medical Center 575 Elmwood Avenue Rochester, NY 14642 585-275-1954
==============================Original Headers============================== 7, 44 -- From Karen_Bentley-at-URMC.Rochester.edu Mon Feb 23 12:37:08 2015 7, 44 -- Received: from voltage3.urmc.rochester.edu (voltage3.urmc.rochester.edu [128.151.10.58]) 7, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1NIb8Vx024893 7, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Feb 2015 12:37:08 -0600 7, 44 -- Received: from proofpoint2.urmc.rochester.edu (proofpoint2.urmc.rochester.edu [128.151.10.141]) 7, 44 -- by voltage3.urmc.rochester.edu (8.14.4/8.14.4) with ESMTP id t1NIb7t9013307 7, 44 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 7, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Feb 2015 13:37:07 -0500 7, 44 -- Received: from pps.filterd (proofpoint2.urmc.rochester.edu [127.0.0.1]) 7, 44 -- by proofpoint2.urmc.rochester.edu (8.14.5/8.14.5) with SMTP id t1NIXoMI005842 7, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Feb 2015 13:37:07 -0500 7, 44 -- Received: from urmcht1.urmc-sh.rochester.edu (urmcht1.urmc.rochester.edu [128.151.10.24]) 7, 44 -- by proofpoint2.urmc.rochester.edu with ESMTP id 1sr5bh8716-1 7, 44 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NOT) 7, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Feb 2015 13:37:07 -0500 7, 44 -- Received: from urmccas3.urmc-sh.rochester.edu (128.151.10.15) by 7, 44 -- urmcht1.urmc-sh.rochester.edu (128.151.10.24) with Microsoft SMTP Server 7, 44 -- (TLS) id 8.3.389.2; Mon, 23 Feb 2015 13:36:54 -0500 7, 44 -- Received: from URMCMS4.urmc-sh.rochester.edu 7, 44 -- ([fe80:0000:0000:0000:2429:259e:33.91.196.25]) by 7, 44 -- urmccas3.urmc-sh.rochester.edu ([172.16.126.201]) with mapi; Mon, 23 Feb 2015 7, 44 -- 13:37:07 -0500 7, 44 -- From: "Bentley, Karen" {Karen_Bentley-at-URMC.Rochester.edu} 7, 44 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 7, 44 -- Date: Mon, 23 Feb 2015 13:37:07 -0500 7, 44 -- Subject: Clinical EM job opening 7, 44 -- Thread-Topic: Clinical EM job opening 7, 44 -- Thread-Index: AdBPlxoGGq/VSZ3hR3+z9GHhe4ew1w== 7, 44 -- Message-ID: {20D976085A319340B0FFF3BED71E93E80609F06EDD-at-URMCMS4.urmc-sh.rochester.edu} 7, 44 -- Accept-Language: en-US 7, 44 -- Content-Language: en-US 7, 44 -- X-MS-Has-Attach: 7, 44 -- X-MS-TNEF-Correlator: 7, 44 -- acceptlanguage: en-US 7, 44 -- Content-Type: text/plain; charset="us-ascii" 7, 44 -- MIME-Version: 1.0 7, 44 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.13.68,1.0.33,0.0.0000 7, 44 -- definitions=2015-02-23_02:2015-02-23,2015-02-23,1970-01-01 signatures=0 7, 44 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 7, 44 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 7, 44 -- engine=7.0.1-1402240000 definitions=main-1502230170 7, 44 -- X-Proofpoint-Outbound-Spam-Details: urmc_outbound_spam_policy 7, 44 -- Content-Transfer-Encoding: 8bit 7, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1NIb8Vx024893 ==============================End of - Headers==============================
Wehnelt apertures are usually selected depending upon the application. High magnification operations are best made with a small aperture, with the filament closer to the front of the Wehnelt, where as for low magnification work, moving the filament back, and using a larger aperture, provides sufficient performance with longer filament life. Those carrying out a large amount of BSE investigations will also be better served using a larger aperture as bigger sources provide higher signals.
Most manufacturers use a smaller Wehnelt aperture than for tungsten, when LaB6 filaments are fitted, as stronger bias fields are required to control the source.
Enjoy
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 20 February 2015 07:31 To: protrain-at-emcourses.com
X-from: hu.duan-at-averydennison.com
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Email: hu.duan-at-averydennison.com Name: Hu Duan
Organization: Avery
Title-Subject: [Filtered] Need your input of diamond knife
Message: Dear microscopists:
I would like to seek you experience about diamond knife. We used to use DDK diamond knife to do cryo sectioning and polishing of soft polymers. Due to supply issue, we would like to switch to another supplier.
With consideration of comparable price and performance, what would you recommend for the replacement? Or which brand/type of diamond knife you have been satisfied with cryo sectioning of soft polymers? Thank you very much.
Hu
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Title-Subject: [Filtered] Northern California Society for Microscopy Spring Meeting
Message: The NCSM spring meeting will be on Thursday, March 19 at Gatan in Pleasanton.
Please join us for dinner and excellent speakers: Ilke Arslan, of PNNL and Brigitte Papahadjopoulos-Sternberg, of NanoAnalytical Lab.
Additional details are on our website: www.ncsmicroscopy.org
We hope to see you on March 19!
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Title-Subject: [Filtered] SEM- Quanta 200 3D Control board
Message: Hi,
We are facing a problem with our FEI Quanta 200 3D (MK1 model). The microscope is extremely slow to respond to the software commands and sometimes never responds at all and the software freezes. When the scope does respond, the pumps stop working after 2 minutes of pumping and\or it is impossible to give any command to the microscope afterward. The FEI maintenance service informed us that we would have to replace the main controller board (PCB, CB2/OPTD) and\or the vaccum control board (PCB, VCB).
Did you experience this kind of problems before? How was it solved? Also, would you know of any used PCB CCB2/OPTD or VCB (from a discarded Quanta 200 3D, MK1) for sale?
Thanks a lot for your help!
Youssef
-------------------------------------- Youssef Chebli, PhD Microscopy and Imaging facility operational manager Institut de recherche en biologie végétale (514 343 6111 #82122) Department of anthropology (514 343 6111 #26901) Université de Montréal
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Hooke College of Applied Sciences, located in Westmont, IL, is offering two courses in electron microscopy.
Scanning Electron Microscopy short course March 23-27, 2015 Transmission Electron Microscopy short course April 7-9, 2015
In addition to lectures, these courses emphasize hands-on training using state-of-the-art equipment.
For further training details and registration information, please follow the link below: http://www.hookecollege.com/
Best regards- __________________________________________________ Chris Gorman Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax)
==============================Original Headers============================== 9, 41 -- From CGorman-at-hookecollege.com Wed Feb 25 14:03:50 2015 9, 41 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 9, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1PK3ofv021153 9, 41 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2015 14:03:50 -0600 9, 41 -- X-ASG-Debug-ID: 1424894629-07bbd413f2a04c0001-4CH8be 9, 41 -- Received: from TMGEX1.tmg.mccrone.com ([192.168.101.73]) by spam.mccrone.com with ESMTP id 7r9TvYXYvytMTDMf for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2015 14:03:49 -0600 (CST) 9, 41 -- X-Barracuda-Envelope-From: CGorman-at-hookecollege.com 9, 41 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by 9, 41 -- TMGEX1.tmg.mccrone.com (192.168.101.73) with Microsoft SMTP Server (TLS) id 9, 41 -- 15.0.913.22; Wed, 25 Feb 2015 14:03:48 -0600 9, 41 -- Received: from TMGEX1.tmg.mccrone.com ([::1]) by TMGEX1.tmg.mccrone.com 9, 41 -- ([fe80::5c:a323:e462:160%14]) with mapi id 15.00.0913.011; Wed, 25 Feb 2015 9, 41 -- 14:03:48 -0600 9, 41 -- From: Christine Gorman {CGorman-at-hookecollege.com} 9, 41 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 41 -- Subject: Electron Microscopy Short Courses 9, 41 -- Thread-Topic: Electron Microscopy Short Courses 9, 41 -- X-ASG-Orig-Subj: Electron Microscopy Short Courses 9, 41 -- Thread-Index: AdBRNiqxBg7FH/TASjiw5ts7vhaydA== 9, 41 -- Date: Wed, 25 Feb 2015 20:03:48 +0000 9, 41 -- Message-ID: {5d0182fcaa204b06bf6e758b2d531a2d-at-TMGEX1.tmg.mccrone.com} 9, 41 -- Accept-Language: en-US 9, 41 -- Content-Language: en-US 9, 41 -- X-MS-Has-Attach: 9, 41 -- X-MS-TNEF-Correlator: 9, 41 -- x-originating-ip: [192.168.101.154] 9, 41 -- Content-Type: text/plain; charset="us-ascii" 9, 41 -- MIME-Version: 1.0 9, 41 -- X-Barracuda-Connect: UNKNOWN[192.168.101.73] 9, 41 -- X-Barracuda-Start-Time: 1424894629 9, 41 -- X-Barracuda-URL: https://spam.mccrone.com:443/cgi-mod/mark.cgi 9, 41 -- X-Virus-Scanned: by bsmtpd at mccrone.com 9, 41 -- X-Barracuda-BRTS-Status: 1 9, 41 -- X-Barracuda-Spam-Score: 0.00 9, 41 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 9, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.15841 9, 41 -- Rule breakdown below 9, 41 -- pts rule name description 9, 41 -- ---- ---------------------- -------------------------------------------------- 9, 41 -- Content-Transfer-Encoding: 8bit 9, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1PK3ofv021153 ==============================End of - Headers==============================
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Title-Subject: [Filtered] LEO 435 VP light panel error
Message: Hi Group!! I have a Leo 435 VP SEM and recently had some imaging problems. I noticed that on the light panel on the back of the instrument, lights #7 and 9 are blinking when the beam is on, but not when it is off or on standby. I know this is an older instrument but does anyone know what these lights indicate (i.e. what circuit board or power supply has failed)?
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==============================Original Headers============================== 10, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 25 18:34:54 2015 10, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1Q0YspU014583 10, 17 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2015 18:34:54 -0600 10, 17 -- Message-ID: {54EE6A2E.6080108-at-microscopy.com} 10, 17 -- Date: Wed, 25 Feb 2015 18:34:54 -0600 10, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 10, 17 -- MIME-Version: 1.0 10, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 17 -- Subject: viaWWW:LEO 435 VP light panel error 10, 17 -- References: {201502251653.t1PGrNCL016237-at-ns.microscopy.com} 10, 17 -- In-Reply-To: {201502251653.t1PGrNCL016237-at-ns.microscopy.com} 10, 17 -- X-Forwarded-Message-Id: {201502251653.t1PGrNCL016237-at-ns.microscopy.com} 10, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear colleagues- As part of a pitch to install remote viewing / webinar capabilities in our microscopy lab, my administration as asked me to find example systems and collect information to help us optimize and balance our wants, needs, and costs. If you have an electron microscope that you can remotely view, remotely operate, send the display output to a classroom, send the display outside your institution's network for a webinar, etc. and are willing to answer some questions about components and costs would you please contact me at brachfelds-at-mail.montclair.edu. Thank you for your time and your help, Stefanie
==============================Original Headers============================== 1, 30 -- From brachfelds-at-mail.montclair.edu Thu Feb 26 11:32:04 2015 1, 30 -- Received: from smtp.montclair.edu (smtp.montclair.edu [130.68.1.64]) 1, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1QHW4Ui020108 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 11:32:04 -0600 1, 30 -- Received: from smtp.montclair.edu (pmx-smtp1.montclair.edu [127.0.0.1]) 1, 30 -- by localhost (Postfix) with SMTP id 2992B26572 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 12:32:04 -0500 (EST) 1, 30 -- Received: from convergence2.montclair.edu (smtp.montclair.edu [130.68.1.64]) 1, 30 -- by smtp.montclair.edu (Postfix) with ESMTP id E4CFA2596C 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 12:32:03 -0500 (EST) 1, 30 -- MIME-version: 1.0 1, 30 -- Content-transfer-encoding: 7BIT 1, 30 -- Content-disposition: inline 1, 30 -- Content-type: text/plain; CHARSET=US-ASCII 1, 30 -- Received: from montclair.edu ([127.0.0.1]) by convergence2.montclair.edu 1, 30 -- (Oracle Communications Messaging Server 7u4-25.01(7.0.4.25.0) 64bit (built Feb 1, 30 -- 29 2012)) with ESMTPA id {0NKE0029G3DFT100-at-convergence2.montclair.edu} for 1, 30 -- Microscopy-at-microscopy.com; Thu, 26 Feb 2015 12:32:03 -0500 (EST) 1, 30 -- Received: from [127.0.0.1] (Forwarded-For: 130.68.125.143) 1, 30 -- by convergence2.montclair.edu (mshttpd); Thu, 26 Feb 2015 12:32:03 -0500 1, 30 -- From: "Stefanie A. Brachfeld" {brachfelds-at-mail.montclair.edu} 1, 30 -- To: Microscopy-at-microscopy.com 1, 30 -- Message-id: {76b087d53a6d28d9.54ef1243-at-montclair.edu} 1, 30 -- Date: Thu, 26 Feb 2015 12:32:03 -0500 1, 30 -- X-Mailer: Oracle Communications Messenger Express 7u4-25.01(7.0.4.25.0) 64bit 1, 30 -- (built Feb 29 2012) 1, 30 -- Content-language: en 1, 30 -- Subject: Hardware and software for remote viewing of SEM and TEM displays 1, 30 -- X-Accept-Language: en 1, 30 -- Priority: normal ==============================End of - Headers==============================
Telepresence Collaboration which is fairly old. We started doing it at ANL when the Mosaic WWW browser first appeared ~ 1994.
There are also a few old PDF's of Lectures here:
http://tpm.amc.anl.gov/Lectures/
Today, there are numerous solutions that update these older protocols.
To be honest, because of network safety (i.e. hacker) issues, I no longer allow remote control, only passive observation. I would caution you that you will need to do the same. All publically accessible sites get attacked. You don't want to know how many hackers try to break into the Microscopy Listserver every DAY.
Nestor Your Friendly Neighborhood SysOp
On Feb 26, 2015, at 11:32 AM CST, brachfelds-at-mail.montclair.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues- } As part of a pitch to install remote viewing / webinar capabilities in our microscopy lab, my administration as asked me to find example systems and collect information to help us optimize and balance our wants, needs, and costs. If you have an electron microscope that you can remotely view, remotely operate, send the display output to a classroom, send the display outside your institution's network for a webinar, etc. and are willing to answer some questions about components and costs would you please contact me at brachfelds-at-mail.montclair.edu. } Thank you for your time and your help, } Stefanie } } ==============================Original Headers============================== } 1, 30 -- From brachfelds-at-mail.montclair.edu Thu Feb 26 11:32:04 2015 } 1, 30 -- Received: from smtp.montclair.edu (smtp.montclair.edu [130.68.1.64]) } 1, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1QHW4Ui020108 } 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 11:32:04 -0600 } 1, 30 -- Received: from smtp.montclair.edu (pmx-smtp1.montclair.edu [127.0.0.1]) } 1, 30 -- by localhost (Postfix) with SMTP id 2992B26572 } 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 12:32:04 -0500 (EST) } 1, 30 -- Received: from convergence2.montclair.edu (smtp.montclair.edu [130.68.1.64]) } 1, 30 -- by smtp.montclair.edu (Postfix) with ESMTP id E4CFA2596C } 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 12:32:03 -0500 (EST) } 1, 30 -- MIME-version: 1.0 } 1, 30 -- Content-transfer-encoding: 7BIT } 1, 30 -- Content-disposition: inline } 1, 30 -- Content-type: text/plain; CHARSET=US-ASCII } 1, 30 -- Received: from montclair.edu ([127.0.0.1]) by convergence2.montclair.edu } 1, 30 -- (Oracle Communications Messaging Server 7u4-25.01(7.0.4.25.0) 64bit (built Feb } 1, 30 -- 29 2012)) with ESMTPA id {0NKE0029G3DFT100-at-convergence2.montclair.edu} for } 1, 30 -- Microscopy-at-microscopy.com; Thu, 26 Feb 2015 12:32:03 -0500 (EST) } 1, 30 -- Received: from [127.0.0.1] (Forwarded-For: 130.68.125.143) } 1, 30 -- by convergence2.montclair.edu (mshttpd); Thu, 26 Feb 2015 12:32:03 -0500 } 1, 30 -- From: "Stefanie A. Brachfeld" {brachfelds-at-mail.montclair.edu} } 1, 30 -- To: Microscopy-at-microscopy.com } 1, 30 -- Message-id: {76b087d53a6d28d9.54ef1243-at-montclair.edu} } 1, 30 -- Date: Thu, 26 Feb 2015 12:32:03 -0500 } 1, 30 -- X-Mailer: Oracle Communications Messenger Express 7u4-25.01(7.0.4.25.0) 64bit } 1, 30 -- (built Feb 29 2012) } 1, 30 -- Content-language: en } 1, 30 -- Subject: Hardware and software for remote viewing of SEM and TEM displays } 1, 30 -- X-Accept-Language: en } 1, 30 -- Priority: normal } ==============================End of - Headers==============================
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center NanoScience and Technology Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
Francoise Marga asked about imaging collagen fibers by SEM. ASM has been working with collagen fibers and monomers for over 20 years, imaging them using AFM(atomic force microscopy). The example images found on our website at:
show that AFM can image individual polymer molecules and can show subtle height variations in the fibers.
I suggest that AFM be considered for the needed analysis.
Disclosure: ASM is an independent analytical service laboratory specializing in Atomic Force Microscopy and related Scanning Probe Microscopy techniques.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: 317-895-5630 Toll free: 800-374-8557 (in USA & Canada) Fax: 317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
----- Original Message ----- From: oshel1pe-at-cmich.edu To: donc-at-asmicro.com Sent: Monday, January 12, 2015 4:59 PM Subject: [a] [Microscopy] Ask-A-Microscopist: SEM service available in Brooklyn NY area for
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Realname - Francoise Marga Email - fmarga-at-modernmeadow.com ORGANIZATION - Modern Meadow EDUCATION - Graduate College LOCATION - Brooklyn, NY, USA SUBJECT_OF_QUESTION - SEM in NYC QUESTION - Hi,
Our company would like to look at our samples by SEM. We need to go up x15,000 to visualize collagen fibers. As a business, we have trouble to find a SEM accessible to a private company. Does anyone know a facility or a private service in our area (Brooklyn, NY) that could help us. Thanks for your help. Kind regards,
Francoise
==============================Original Headers============================== 6, 31 -- From oshel1pe-at-cmich.edu Mon Jan 12 15:53:10 2015 6, 31 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0CLrAld023845 6, 31 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2015 15:53:10 -0600 6, 31 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 6, 31 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0CLrAee031293 6, 31 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2015 16:53:10 -0500 6, 31 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 6, 31 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with
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Title-Subject: [Filtered] Preventing charging in a TEM
Message: Hello, Does anybody use a coater to coat ceramic TEM samples with a very thin layer to mitigate charging? I would also be happy to know if there are any other ways to stop charging of samples. I am looking at manually polished complex oxide samples. Thanks! --------------------------------------------- Debangshu Mukherjee Materials Research Institute The Pennsylvania State University N-303 Millennium Science Complex University Park, PA 16802-2130 Phone: (617) 501-7316 ----------------------------------------------
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==============================Original Headers============================== 15, 16 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 28 08:02:14 2015 15, 16 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1SE2EPw010681 15, 16 -- for {microscopy-at-microscopy.com} ; Sat, 28 Feb 2015 08:02:14 -0600 15, 16 -- Message-ID: {54F1CA66.909-at-microscopy.com} 15, 16 -- Date: Sat, 28 Feb 2015 08:02:14 -0600 15, 16 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 16 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 16 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 15, 16 -- MIME-Version: 1.0 15, 16 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 16 -- Subject: viaWWW: Preventing charging in a TEM 15, 16 -- References: {201502272306.t1RN65mp030923-at-ns.microscopy.com} 15, 16 -- In-Reply-To: {201502272306.t1RN65mp030923-at-ns.microscopy.com} 15, 16 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We've had good luck putting down a few nm (usually 3nm on our system) of Carbon to mitigate charging and to stabilize the formvar films our bio friends make. If you are doing atomic resolution STEM, make sure the C is on the bottom side.
Cheers, Henk
On 2/28/2015 9:05 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from:debangshu-at-psu.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy bothdebangshu-at-psu.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email:debangshu-at-psu.edu } Name: Debangshu Mukherjee } } Organization: The Pennsylvania State University } } Title-Subject: [Filtered] Preventing charging in a TEM } } Message: Hello, } Does anybody use a coater to coat ceramic TEM samples with a very thin layer to mitigate charging? } I would also be happy to know if there are any other ways to stop charging of samples. I am looking } at manually polished complex oxide samples. } Thanks! } --------------------------------------------- } Debangshu Mukherjee } Materials Research Institute } The Pennsylvania State University } N-303 Millennium Science Complex } University Park, PA 16802-2130 } Phone: (617) 501-7316 } ---------------------------------------------- } } Login Host: 104.39.17.35 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
I assume that Debangshu is using the highest accelerating voltage that he can with his instrument, and probably small condenser apertures and spot sizes.
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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==============================Original Headers============================== 7, 22 -- From protrain-at-emcourses.com Mon Mar 2 04:51:26 2015 7, 22 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t22ApPgq017562 7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2015 04:51:26 -0600 7, 22 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 7, 22 -- id {B54f440a60000} ; Mon, 02 Mar 2015 10:51:18 +0000 7, 22 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Mon, 2 Mar 2015 10:51:17 +0000 7, 22 -- From: "Steve Chapman" {protrain-at-emcourses.com} 7, 22 -- To: {colijn.1-at-osu.edu} , {debangshu-at-psu.edu} 7, 22 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 7, 22 -- References: {201502281753.t1SHrdm1002327-at-ns.microscopy.com} 7, 22 -- In-Reply-To: {201502281753.t1SHrdm1002327-at-ns.microscopy.com} 7, 22 -- Subject: RE: [Microscopy] Re: viaWWW: Preventing charging in a TEM 7, 22 -- Date: Mon, 2 Mar 2015 10:51:21 -0000 7, 22 -- Message-ID: {00b601d054d6$d228fb20$767af160$-at-com} 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: text/plain; 7, 22 -- charset="us-ascii" 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- X-Mailer: Microsoft Office Outlook 12.0 7, 22 -- Thread-index: AdBTf3tmVMFuO1OpTA6cBWaHIm6mZgBVoOAw 7, 22 -- Content-language: en-gb ==============================End of - Headers==============================
Hello Since the 90ies I use double sided adhesive tape for mounting SEM specimens and remember how strong these early tapes were. The last years I tried many different suppliers and all (carbon or copper) tapes I get are not very sticky, resulting in charging and beam instability problems that gives a headache. Anybody knows where to get a double sided adhesive tape for mounting SEM specimens that is really sticky? Thanks
I have a Gatan PIPS II system with Digital Micrograph. Does anyone know if there is a DM script available that can auto-terminate the ion milling process when perforation occurs? Â
-Scott Walck
==============================Original Headers============================== 3, 35 -- From s.walck-at-comcast.net Wed Mar 4 03:30:30 2015 3, 35 -- Received: from resqmta-ch2-09v.sys.comcast.net (resqmta-ch2-09v.sys.comcast.net [69.252.207.41]) 3, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t249UUUe018714 3, 35 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2015 03:30:30 -0600 3, 35 -- Received: from resomta-ch2-11v.sys.comcast.net ([69.252.207.107]) 3, 35 -- by resqmta-ch2-09v.sys.comcast.net with comcast 3, 35 -- id zMWW1p0012Ka2Q501MWWhj; Wed, 04 Mar 2015 09:30:30 +0000 3, 35 -- Received: from resmail-ch2-280v.sys.comcast.net ([162.150.49.59]) 3, 35 -- by resomta-ch2-11v.sys.comcast.net with comcast 3, 35 -- id zMWW1p0031GdifW01MWWvU; Wed, 04 Mar 2015 09:30:30 +0000 3, 35 -- Date: Wed, 4 Mar 2015 09:30:29 +0000 (UTC) 3, 35 -- From: "S.Walck" {s.walck-at-comcast.net} 3, 35 -- To: MicroscopyListserver {microscopy-at-microscopy.com} 3, 35 -- Message-ID: {1078403573.3352557.1425461429944.JavaMail.zimbra-at-comcast.net} 3, 35 -- In-Reply-To: {1718498827.3352392.1425461389483.JavaMail.zimbra-at-comcast.net} 3, 35 -- Subject: Auto-Termination Script for PIPS II 3, 35 -- MIME-Version: 1.0 3, 35 -- Content-Type: text/plain; charset=utf-8 3, 35 -- X-Originating-IP: [2601:a:6300:b917:7423:6014:1f43:8e05] 3, 35 -- X-Mailer: Zimbra 8.0.7_GA_6031 (ZimbraWebClient - IE7 (Win)/8.0.7_GA_6031) 3, 35 -- Thread-Topic: Auto-Termination Script for PIPS II 3, 35 -- Thread-Index: nncZUZ7jGz9gQIO9MAzq3R4Lckoqng== 3, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 3, 35 -- s=q20140121; t=1425461430; 3, 35 -- bh=np+b6KnP/8E9XnAXXPFalShgkLCzWyEtXT7Z20sdI5A=; 3, 35 -- h=Received:Received:Date:From:To:Message-ID:Subject:MIME-Version: 3, 35 -- Content-Type; 3, 35 -- b=CeAAgBSUNa9j8DWm/LM4VGgu/o/d7xm2R37yact1fDha9itcTiv2oURkKSjUtT0rs 3, 35 -- dlecRYPa8N1e4sGRLEDGi+1BtHY0+WtjL63abcoL0y/eA1xqT5i6paGjw6nAS/Tl7I 3, 35 -- ZuGsrpYSXO+PODk8uqccPA5zRVaki49CxEhlDXXxtOSfqhWoUCSVbvez56gRBcHnHc 3, 35 -- LmXG30t5zgQWO3okkr3VsLmOJNA8jLi1/2/iFrNvxYSW8aEmRH3BlLc33K9QWHIWik 3, 35 -- qM705L9SWrUUe/LGu1ORutuRY60uN5VG8xF3XZFqr+e0tU2rjbPfsaGA//aAN9IpYc 3, 35 -- fkhPIwsq8URgA== 3, 35 -- Content-Transfer-Encoding: 8bit 3, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t249UUUe018714 ==============================End of - Headers==============================
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Email: jarmstrong-at-ciw.edu Name: JOHN T ARMSTRONG
Organization: Carnegie Institution of Washington - Geophysical Laboratory
Title-Subject: [Filtered] Endowed support staff position - Microbeam specialist - available
Message: CARNEGIE INSTITUTION OF WASHINGTON - Vacancy Announcement ÂMicrobeam Specialist
The Microbeam Specialist will work at the Geophysical Laboratory location in Washington, D.C.
Job Description:
The primary responsibility is to maintain and operate focused ion beam - scanning electron microscope (FIB-SEM) crossbeam system and other microbeam instruments, including training and providing assistance to new and visiting users, performing routine maintenance, sample preparation, and collaborating with staff and students. As a member of the electronics/microbeam analysis center, the person is expected to work with existing microbeam members to maintain smooth operation of the facility. The Laboratory supports world-class facilities in high-pressure research; organic, stable isotope and biogeochemistry; mineral physics and petrology; and astrobiology. See https://www.gl.ciw.edu/ for a listing of its research programs and facilities.
See details at https://www.gl.ciw.edu/content/2015/2/26/microbeam-specialist
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==============================Original Headers============================== 21, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Mar 4 06:02:41 2015 21, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 21, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t24C2fLV008326 21, 17 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2015 06:02:41 -0600 21, 17 -- Message-ID: {54F6F461.1060108-at-microscopy.com} 21, 17 -- Date: Wed, 04 Mar 2015 06:02:41 -0600 21, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 21, 17 -- MIME-Version: 1.0 21, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 17 -- Subject: viaWWW:Endowed support staff position - Microbeam specialist - available 21, 17 -- References: {201503032028.t23KSe2x016429-at-ns.microscopy.com} 21, 17 -- In-Reply-To: {201503032028.t23KSe2x016429-at-ns.microscopy.com} 21, 17 -- X-Forwarded-Message-Id: {201503032028.t23KSe2x016429-at-ns.microscopy.com} 21, 17 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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} realname - Paul Webster } Email - pwebster-at-usc.edu } EDUCATION - Graduate College } LOCATION - Pasadena, CA 91107, USA } SUBJECT_OF_QUESTION - Critical Point Dryer } QUESTION - Dear All, } } I have an old Balzers Union critical point dryer FL 9496 that has been working well until now. } } The problem is that the lid of the chamber has started to leak. It may just need a gasket, but I have no idea where I could get one. } } Does anyone have information on where I could find a gasket for this part. Failing that, a source for a new lid would be welcome. } } Perhaps Technotrade is still out there and has parts for these old machines. } } Regards, } } Paul } } Paul Webster, Ph.D. } }
==============================Original Headers============================== 5, 35 -- From oshel1pe-at-cmich.edu Thu Mar 5 07:14:27 2015 5, 35 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t25DERHO026661 5, 35 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 07:14:27 -0600 5, 35 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 35 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t25DEQPf014486 5, 35 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 08:14:26 -0500 5, 35 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 5, 35 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 35 -- (TLS) id 14.3.195.1; Thu, 5 Mar 2015 08:14:26 -0500 5, 35 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 5, 35 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 5, 35 -- (TLS) id 14.3.195.1; Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 35 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 5, 35 -- 14.3.195.1; Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- Message-ID: {54F856B1.1070604-at-cmich.edu} 5, 35 -- Date: Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 35 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 35 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 35 -- MIME-Version: 1.0 5, 35 -- To: micro {microscopy-at-microscopy.com} 5, 35 -- Subject: Re: Ask-A-Microscopist 5, 35 -- References: {985736566.4028.1425508312396.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 35 -- In-Reply-To: {985736566.4028.1425508312396.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 35 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 5, 35 -- Content-Transfer-Encoding: 7bit 5, 35 -- X-Originating-IP: [141.209.2.100] 5, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 35 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 35 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 35 -- X-CanItPRO-Stream: default 5, 35 -- X-Canit-Stats-ID: 02NXNeqly - b4c40e78afdd - 20150305 5, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Don't miss the doodle honoring Momofuku Ando on the Google homepage today. There are different versions but at least one features him working at a microscope! He is presumably looking at the microscopic pores in the ramen noodles that result from flash frying and allows their rapid rehydration upon adding boiling water. Many a hungry grad student slaving over a microscope owes him a debt.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
The only posibility - I guess - to get any spare part for a Balzers CPD of old vintage in Europe (I know of, last correction in my PAB done: 2012/07) would be: ===== Info distributed by PROVAC, former supplier for BALZERS instruments and spare-parts: Date: 6 Nov 2009 "As of October 15th 2009 Baltic Präparation, Niesgrau/Germany takes over the business of Consumables for the Electron Microscopy Preparation Tecnology from Provac AG, Balzers. For many years Provac AG was a reliable partner for sales of consumables for the Elctron Microscopy Preparation Technology and we would like to thank our customers for the good cooperation and the trust in our company. Mrs. Claudia Köster from BALTIC PRÄPERATION will be the new contact for any future inquiries. For further information please contact: Mrs. Claudia Köster Baltic Präparation Koppelheck 34B D-24395 Niesgrau Germany Phone: +49 4643 18 65 43 e-mail: baltic.praeparation-at-t-online.de "
as of the (BALTIC) German website 2015-03-05: Postal address: Baltic Präparation Postfach 1116 D-24393 Koppelheck Fon 04643/186543 Fax 04643/186554 e-mail: baltic.praeparation-at-t-online.de or php-form at http://www.baltic-praeparation.de/kontakt.html
I found only a Website in GERMAN . cf: www.baltic-praeparation.de which (since 2012) still is "under construction" cf also : Products 2009 (lacking any description of a CPD-FL 9496): http://www.baltic-praeparation.de/index.php?file=tl_files/pdfs/procac2.pdf
Not knowing how they perform nowadays.... but I think it would be worth a try to request for the spare part. Best wishes, good luck, and my best regards, Wolfgang
Wolfgang MUSS EM-Lab, Univ.Inst.PATHOLOGY, SALK-LKH (Gen. Hospital) and PMU SALZBURG SALZBURG, AUSTRIA
Von: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Gesendet: Donnerstag, 05. März 2015 14:23 An: Muß Wolfgang Betreff: [Microscopy] Re: Ask-A-Microscopist: Critical Point Dryer (Spare parts for: BALZERS FL 9496)
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} realname - Paul Webster } Email - pwebster-at-usc.edu } EDUCATION - Graduate College } LOCATION - Pasadena, CA 91107, USA } SUBJECT_OF_QUESTION - Critical Point Dryer } QUESTION - } } Dear All, } I have an old Balzers Union critical point dryer FL 9496 that has been working well until now. } } The problem is that the lid of the chamber has started to leak. It may just need a gasket, but I have no idea where I could get one. } } Does anyone have information on where I could find a gasket for this part. Failing that, a source for a new lid would be welcome. } } Perhaps Technotrade is still out there and has parts for these old machines. } } Regards, } } Paul } } Paul Webster, Ph.D.
==============================Original Headers============================== 5, 35 -- From oshel1pe-at-cmich.edu Thu Mar 5 07:14:27 2015 5, 35 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t25DERHO026661 5, 35 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 07:14:27 -0600 5, 35 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 35 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t25DEQPf014486 5, 35 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 08:14:26 -0500 5, 35 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 5, 35 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 35 -- (TLS) id 14.3.195.1; Thu, 5 Mar 2015 08:14:26 -0500 5, 35 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 5, 35 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 5, 35 -- (TLS) id 14.3.195.1; Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 35 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 5, 35 -- 14.3.195.1; Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- Message-ID: {54F856B1.1070604-at-cmich.edu} 5, 35 -- Date: Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 35 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 35 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 35 -- MIME-Version: 1.0 5, 35 -- To: micro {microscopy-at-microscopy.com} 5, 35 -- Subject: Re: Ask-A-Microscopist 5, 35 -- References: {985736566.4028.1425508312396.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 35 -- In-Reply-To: {985736566.4028.1425508312396.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 35 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 5, 35 -- Content-Transfer-Encoding: 7bit 5, 35 -- X-Originating-IP: [141.209.2.100] 5, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 35 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 35 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 35 -- X-CanItPRO-Stream: default 5, 35 -- X-Canit-Stats-ID: 02NXNeqly - b4c40e78afdd - 20150305 5, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 43 -- From W.Muss-at-salk.at Thu Mar 5 08:52:44 2015 15, 43 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 15, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t25EqijD029551 15, 43 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 08:52:44 -0600 15, 43 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 15, 43 -- by hermes.salk.at (Postfix) with ESMTP id 23CD8646B87 15, 43 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 15:52:43 +0100 (CET) 15, 43 -- X-Scanned-By: SALK-Content-Filter 15, 43 -- Received: from hermes.salk.at ([127.0.0.1]) 15, 43 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 15, 43 -- with ESMTP id YHf29aeiiPZs for {Microscopy-at-microscopy.com} ; 15, 43 -- Thu, 5 Mar 2015 15:52:42 +0100 (CET) 15, 43 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 15, 43 -- by hermes.salk.at (Postfix) with ESMTP id D2F9E646B9E 15, 43 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 15:52:42 +0100 (CET) 15, 43 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 15, 43 -- by N1EX198.lks.local with ESMTP; 05 Mar 2015 16:04:03 +0100 15, 43 -- Received: from N1DT132.lksdom21.lks.local ([fe80::c9bf:82a7:c92e:ef25]) by 15, 43 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 15, 43 -- 14.03.0224.002; Thu, 5 Mar 2015 15:52:42 +0100 15, 43 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 15, 43 -- To: "'pwebster-at-usc.edu'" {pwebster-at-usc.edu} , 15, 43 -- "'Microscopy-at-microscopy.com'" 15, 43 -- {Microscopy-at-microscopy.com} 15, 43 -- CC: "'oshel1pe-at-cmich.edu'" {oshel1pe-at-cmich.edu} 15, 43 -- Subject: [Microscopy] Re: Ask-A-Microscopist: Critical Point Dryer (Spare 15, 43 -- parts for: BALZERS FL 9496) 15, 43 -- Thread-Topic: [Microscopy] Re: Ask-A-Microscopist: Critical Point Dryer 15, 43 -- (Spare parts for: BALZERS FL 9496) 15, 43 -- Thread-Index: AQHQV0eQR5NIPI/HPEWFTKhMolWKMJ0N9QFw 15, 43 -- Date: Thu, 5 Mar 2015 14:52:42 +0000 15, 43 -- Message-ID: {3D22E6EE65F39E448DCE41628153767133DCA867-at-N1DT132.lksdom21.lks.local} 15, 43 -- References: {201503051323.t25DNDT7031073-at-ns.microscopy.com} 15, 43 -- In-Reply-To: {201503051323.t25DNDT7031073-at-ns.microscopy.com} 15, 43 -- Accept-Language: de-DE, en-US 15, 43 -- Content-Language: de-DE 15, 43 -- X-MS-Has-Attach: 15, 43 -- X-MS-TNEF-Correlator: 15, 43 -- x-originating-ip: [192.168.42.2] 15, 43 -- Content-Type: text/plain; charset="iso-8859-1" 15, 43 -- MIME-Version: 1.0 15, 43 -- Content-Transfer-Encoding: 8bit 15, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t25EqijD029551 ==============================End of - Headers==============================
I survived for almost half a decade on raman noodles and learned many ways to make them including stir frying with Napa cabbage and veggies to at least pretend that it was a different dinner than the last 4000 raman noodle dinners I had. The generic ones were 8 for one buck and between that and bulk pinto beans, we managed to survive on our meager food budget which was inadequate to pay for all the beer we drank and the food too. :)
Sincerely,
James Neal-Kababick Director Flora Research Laboratories, LLC An FDA and DEA Registered & Inspected Laboratory Fellow AOAC International Vice Chair USP {2251} ADSDDA Expert Panel Adjunct Faculty Bastyr University Botanical Medicine Department 1-541-472-0980 phone jimk-at-floraresearch.com www.floraresearch.com
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-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Thursday, March 05, 2015 6:58 AM To: James Neal-Kababick
Don't miss the doodle honoring Momofuku Ando on the Google homepage today. There are different versions but at least one features him working at a microscope! He is presumably looking at the microscopic pores in the ramen noodles that result from flash frying and allows their rapid rehydration upon adding boiling water. Many a hungry grad student slaving over a microscope owes him a debt.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
A Postdoctoral position in immediately available at Northwestern University in the group of L. D. Marks (www.numis.northwestern.edu) to work in the area of tribology at the nanoscale with spillover into issues related to hip implants such as tribocorrosion and wear. While most of the work will be materials science based, aspects of it will involve collaborations with orthopedic and dental researchers and doctors. Strong experimental skills in transmission electron microscopy are essential, and experience with using Nanofactory holders (both STM and AFM) would be significant. While a background in metallurgy could be useful, it is not essential; more important is the ability to learn and think outside the box.
Please send a CV and the name of three referees to L. D. Marks L-marks-at-northwestern.edu, email only.
-- Professor Laurence Marks Department of Materials Science and Engineering Northwestern University www.numis.northwestern.edu Corrosion in 4D: MURI4D.numis.northwestern.edu Co-Editor, Acta Cryst A "Research is to see what everybody else has seen, and to think what nobody else has thought" Albert Szent-Gyorgi
==============================Original Headers============================== 4, 26 -- From marksmsa-at-gmail.com Thu Mar 5 18:50:53 2015 4, 26 -- Received: from mail-wi0-f173.google.com (mail-wi0-f173.google.com [209.85.212.173]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t260or8a022676 4, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 18:50:53 -0600 4, 26 -- Received: by wibhm9 with SMTP id hm9so39847wib.2 4, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 05 Mar 2015 16:50:52 -0800 (PST) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=20120113; 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 26 -- bh=rESq0Vp9Zoex6eYrZTxUjYnSx7vsc53BBI/HbCIGqng=; 4, 26 -- b=Qc3LFWjxoka2o7Gbw6nG/MWYPzziOIyuBrpWiY0D+iBCNoPCf9POYklDoyI9Dlsxzq 4, 26 -- 5vM05aHp0stI2N64t1AgKJBq6nfhiQrI6YSiMxfdaHhwCudYQt9hwglVQ39vTACmmz5L 4, 26 -- s+fXbpjBF+7Ug3MILxxXVX43DaKarwLrkER1MdJUAA1KPER1OFzsDpR3v1lv7rizJJpH 4, 26 -- rvxB5QRLh0HW7ONIlbKyqgnRg8Z6Y/YQkHFEQBO/mGmdhQvhiLVU9jeqvVw5STaXU7ro 4, 26 -- TFruWWmsrWESJI1M4AdX8xUcBxoWZby7jyULzhAF72OU1jJbjfzdHU9zlLuM/RrmZnW/ 4, 26 -- j3SA== 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Received: by 10.180.14.7 with SMTP id l7mr70336403wic.40.1425587600135; Thu, 4, 26 -- 05 Mar 2015 12:33:20 -0800 (PST) 4, 26 -- Received: by 10.194.156.234 with HTTP; Thu, 5 Mar 2015 12:33:20 -0800 (PST) 4, 26 -- Date: Thu, 5 Mar 2015 14:33:20 -0600 4, 26 -- Message-ID: {CAHuu12otiawVsZTO67s3qhV_GeVD=0gzCZ_Jw-dOE5jzNuYqyg-at-mail.gmail.com} 4, 26 -- Subject: From Tribology to Hip Implants: Postdoctoral Position 4, 26 -- From: L Marks {marksmsa-at-gmail.com} 4, 26 -- To: microscopy {Microscopy-at-microscopy.com} 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
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Email: mattinson-at-geology.cwu.edu Name: Chris Mattinson
Organization: Central Washington University
Title-Subject: [Filtered] SEM - Recommendation on C-coater, CL detector
Message: We are looking to buy a C-coater to be used with a FE-SEM, and would be grateful for your comments/experiences with C-coaters such as Cressington 208C, Denton Desk V, Quorumtech Q150T, or others you may recommend. Our coater would primarily be used to prepare geological samples for EDS analysis and mapping, including quantitative EDS analysis using standards and beam current measurement, so the evenness and reproducibility of the coating are important.
Also, we are considering the Centaurus CL detector for the SEM, and would appreciate input from anyone who has experience with this detector.
Thanks, Chris Mattinson Central Washington University
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Email: schenderson-at-vcu.edu Name: Scott Henderson
Organization: Virginia Commonwealth University
Title-Subject: [Filtered] position available - Microscopy Technician
Message: Microscopy Technician
A technical position is available in the Microscopy Facility of the Department of Anatomy and Neurobiology in the School of Medicine at Virginia Commonwealth University. The facility houses confocal (laser scanning & spinning disc), multi-photon, TIRF, super-resolution / SIM and electron microscopes (TEM & SEM). The successful candidate will assist with microscopy studies of various biological systems. Duties include assisting users of the facility, providing basic instruction in the use of equipment within the facility (i.e. microscopes, microtomes, and image analysis programs), sample preparation (e.g. sectioning, staining), minor equipment maintenance and some administrative work (ordering of supplies and monthly billing). Applicants should have excellent communication and organizational skills, an understanding of laboratory procedures, and the ability to manage a large and varied workload. Minimum qualifications include a bachelorÂs degree in Science (with a concentration in Biology), previous hands-on experience with advanced light microscopy (e.g. confocal), electron microscopy, sample preparation (including sectioning), and image analysis. Computer skills are essential.
To apply, go to the VCU Jobs website at: https://www.vcujobs.com/
Click the Search Postings tab. The position number is 551220.
--------------------------
Scott Henderson, Ph.D. Director, VCU Microscopy Facility Associate Professor Dept. Anatomy & Neurobiology Virginia Commonwealth University School of Medicine 1101 East Marshall St. Richmond, VA 23298-0709
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Message: Hello, I am preparing cross-sectional TEM samples of pyroelectric materials, which crack when I am curing my epoxy. It would be great if anyone can point me towards recommended examples of room-temperature epoxies available. Thanks!
Debangshu Mukherjee PhD candidate MatSE, Penn State
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Sat Mar 7 09:58:10 2015 14, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t27FwAw7003917 14, 17 -- for {microscopy-at-microscopy.com} ; Sat, 7 Mar 2015 09:58:10 -0600 14, 17 -- Message-ID: {54FB2012.6090702-at-microscopy.com} 14, 17 -- Date: Sat, 07 Mar 2015 09:58:10 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:Room Temperature Epoxy 14, 17 -- References: {201503070641.t276fKGQ026848-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201503070641.t276fKGQ026848-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201503070641.t276fKGQ026848-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On Mar 7, 2015, at 8:35 AM, microscopylistserver- noreply-at-microscopy.com wrote:
} Name: Chris Mattinson } } Organization: Central Washington University } } Title-Subject: [Filtered] SEM - Recommendation on C-coater, CL } detector } } Message: We are looking to buy a C-coater to be used with a FE-SEM, } and would be grateful for your } comments/experiences with C-coaters such as Cressington 208C, Denton } Desk V, Quorumtech Q150T, or } others you may recommend. Our coater would primarily be used to } prepare geological samples for EDS } analysis and mapping, including quantitative EDS analysis using } standards and beam current } measurement, so the evenness and reproducibility of the coating are } important. } } Also, we are considering the Centaurus CL detector for the SEM, and } would appreciate input from } anyone who has experience with this detector. } } Thanks,
Dear Chris, I used the Cressington 208 for both carbon and metal coating, and it was very reliable and consistent. Just a satisfied customer; no financial interest. Yours, Bill
==============================Original Headers============================== 8, 32 -- From wtivol-at-sbcglobal.net Sat Mar 7 18:49:22 2015 8, 32 -- Received: from nm21-vm2.access.bullet.mail.gq1.yahoo.com (nm21-vm2.access.bullet.mail.gq1.yahoo.com [216.39.63.49]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t280nLD9013097 8, 32 -- for {microscopy-at-microscopy.com} ; Sat, 7 Mar 2015 18:49:21 -0600 8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1425775760; bh=gvZP17A01J4ymZw2T5vtdyDudnTwD79SBFxtIBsOASw=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=IjkZWvuqBs1KHZ0XJldoGnxo8GdfC8VVpDhuXtCkzN22MG7Bd9U7VRFksa2Go+YrAxUeyMxsS5Y2HHlI9YMBVl1dRWVwe/99UixqG7GGnVJTIf+r5Q1D1hxLAmG8gRzHPxo+VMQ0octyi+TPQRZSlHxhSeLF89E98hJKKmIdB16l17JRdzFLBy6h0FD7hbaHJzu0dm8CLwF9lQPw5SNMU4NWIgl/3LztCGxqHn43kocmpBSiPPBZjtK0h2bAhlL9ti4xOQ7MAKyy0mUtVE5mDjt6SWBbIkI5dC0VVOBNjf6bJcAH+1wKvbI+ZPaLCInI+E8+55bKwtfBgrMDjtLlAw== 8, 32 -- Received: from [216.39.60.170] by nm21.access.bullet.mail.gq1.yahoo.com with NNFMP; 08 Mar 2015 00:49:20 -0000 8, 32 -- Received: from [67.195.22.116] by tm6.access.bullet.mail.gq1.yahoo.com with NNFMP; 08 Mar 2015 00:49:20 -0000 8, 32 -- Received: from [127.0.0.1] by smtp111.sbc.mail.gq1.yahoo.com with NNFMP; 08 Mar 2015 00:49:20 -0000 8, 32 -- X-Yahoo-Newman-Id: 898186.49976.bm-at-smtp111.sbc.mail.gq1.yahoo.com 8, 32 -- X-Yahoo-Newman-Property: ymail-3 8, 32 -- X-YMail-OSG: 8gydN4UVM1m3KGqo_oYIcvjioMLLTxkjYQFqpg8LJtj57a9 8, 32 -- Ne1HMsc8xg6JsA8VGGnkNkpOeng.svuVUoU246mihGZJdCe_UNuAv2IoB4D1 8, 32 -- 43Xd01ld163HwoyB5vZABYcEjY1H.s77ZSCp3mjRfBYzUHKMZCi7_HAZyfCw 8, 32 -- 5JjWgIs.mrtY6pOeqf1swO9MnZANbVB1tIoVNYXxrZEr72Vglqk_xr0P5Ori 8, 32 -- 6FSTdyqjcldSDVIaZl2RHeO4DtSS801ja.zzqYVfA8ZLUP7nhbZcks6AX10m 8, 32 -- UrUNjV10OnOrRz_zGN9lq.nxq1kuxeRukhDKXiHYnmsBptKVhdQ3Vt.plQWy 8, 32 -- A61QW_8ANRwx3rmeEou94jLqC.EHjFksd8tXLbNvA1b07tKK7RPzM2kArlm4 8, 32 -- 8_FTOWrPX92vCAN2QWqT9XhSvAS5DtH7EOkpgq_yfWuL4JPBcpwcYC1BpQ1S 8, 32 -- 5rlwwiv2tehgSSMr9pV0qZqqt8eHHJukeOe1hrdQbFds.cf0TAxDcvAGt73D 8, 32 -- 4C7xFkNb27cjkDmhOWbvqoL.diNiaE7hlMV9lTg-- 8, 32 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 8, 32 -- Message-Id: {01B9058D-F98A-478B-9796-196B4CC067EF-at-sbcglobal.net} 8, 32 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 8, 32 -- To: microscopy-at-microscopy.com, mattinson-at-geology.cwu.edu 8, 32 -- In-Reply-To: {201503071635.t27GZSMZ015996-at-ns.microscopy.com} 8, 32 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 32 -- Content-Transfer-Encoding: 7bit 8, 32 -- Mime-Version: 1.0 (Apple Message framework v936) 8, 32 -- Subject: Re: [Microscopy] viaWWW:SEM - Recommendation on C-coater, CL detector 8, 32 -- Date: Sat, 7 Mar 2015 16:49:18 -0800 8, 32 -- References: {201503071635.t27GZSMZ015996-at-ns.microscopy.com} 8, 32 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
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Email: cmazareanu-at-yahoo.com Name: Constantin
Organization: hobby
Title-Subject: [Filtered] kevex 4855
Message: Hi I am looking for any information regarding kevex 4855 SEM control digital interface. I need setup information and and a schematic if is possible. I searched around internet but no information is available. This is a part of a larger effort to bring a SEm to digital age. I am looking also for KEVEX MCA schematics and setup. Cannot find anywhere KEvex Sigma software. There is someone who can help me? Thank you Constantin
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Sun Mar 8 17:01:19 2015 14, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t28M1INq025100 14, 17 -- for {microscopy-at-microscopy.com} ; Sun, 8 Mar 2015 17:01:18 -0500 14, 17 -- Message-ID: {54FCC6AE.7090401-at-microscopy.com} 14, 17 -- Date: Sun, 08 Mar 2015 17:01:18 -0500 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:kevex 4855 info needed 14, 17 -- References: {201503081559.t28FxZej007013-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201503081559.t28FxZej007013-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201503081559.t28FxZej007013-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We have an issue with our two conventional Gatan heating holders (inconnel and tantalum) and were wondering, if someone else faced the same.
The problem is that we cannot get HRTEM images when the holder is connected to the power supply and the supply is switched on. It doesn't matter if the heating is running or not, it just has to be switched on. The power supply is connected to a socket coming from the microscope and additional grounding doesn't help. Interchanging of the two power supplies makes no difference. Interestingly, this issue is not 100 procent reproducible, as from time to time everything is working perfectly and you see no effect at all. The microscope is a FEI Titan.
Here is a link to a few images. One taken with the power supply switched off and the related FFT, and one with the power supply switched on and the related FFT. (https://onedrive.live.com/redir?resid=507493AEB39D8528!211&authkey=!AHqWGyO oE_yhiCw&ithint=folder%2cjpg)
Did anybody face the same or has an idea about it? Any help is very much appreciated.
Thanks a lot Jens
==============================Original Headers============================== 7, 24 -- From jens.kling-at-web.de Mon Mar 9 07:23:17 2015 7, 24 -- Received: from mout.web.de (mout.web.de [212.227.15.3]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t29CNHEZ001516 7, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2015 07:23:17 -0500 7, 24 -- Received: from CENJens ([130.225.78.50]) by smtp.web.de (mrweb004) with 7, 24 -- ESMTPSA (Nemesis) id 0MNx4F-1YOcFr1pcZ-007XcV for 7, 24 -- {Microscopy-at-microscopy.com} ; Mon, 09 Mar 2015 13:23:16 +0100 7, 24 -- From: "Jens Kling" {jens.kling-at-web.de} 7, 24 -- To: {Microscopy-at-microscopy.com} 7, 24 -- Subject: TEM Gatan heating holder issue 7, 24 -- Date: Mon, 9 Mar 2015 13:23:15 +0100 7, 24 -- Message-ID: {001201d05a63$d1dd7c80$75987580$-at-web.de} 7, 24 -- MIME-Version: 1.0 7, 24 -- Content-Type: text/plain; 7, 24 -- charset="us-ascii" 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- X-Mailer: Microsoft Outlook 14.0 7, 24 -- Thread-Index: AdBaY3QbnkVpQNnKRI+AAy79f8sH+Q== 7, 24 -- Content-Language: en-us 7, 24 -- X-Provags-ID: V03:K0:bMFxPjQpRAB5zt0uiHriJv86s82zFbA8i5W6/kLldFnvuaqDi0S 7, 24 -- 1pvu600FEkpnz0mVvC2kWuqdO/Nv9qpwNXfLKYEnPmV3ymQ5/lg/9NXE9+XHVGXu1hNOwa1 7, 24 -- nG2IpsHHuNjWILMYtGTPOaeum6RX1wwzMWdZKdkI8GWOaZkiONQ8hI67Lv5vEepEKCdWNmK 7, 24 -- euL3rsnTI/ck31VcTR6uQ== 7, 24 -- X-UI-Out-Filterresults: notjunk:1; ==============================End of - Headers==============================
It could be vibrations induced in the connecting cable that are transferred into the holder. If it was electrical interference caused by insufficient or poor grounding, then I would think it wouldn't be intermittent. Try securing the cable and see if it improves the behavior. Otherwise, I would contact Gatan directly to see if they have a better idea.
Good luck, Chris
On Mon, Mar 9, 2015 at 8:35 AM, {jens.kling-at-web.de} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Everyone } } We have an issue with our two conventional Gatan heating holders (inconnel } and tantalum) and were wondering, if someone else faced the same. } } The problem is that we cannot get HRTEM images when the holder is connected } to the power supply and the supply is switched on. It doesn't matter if the } heating is running or not, it just has to be switched on. The power supply } is connected to a socket coming from the microscope and additional grounding } doesn't help. Interchanging of the two power supplies makes no difference. } Interestingly, this issue is not 100 procent reproducible, as from time to } time everything is working perfectly and you see no effect at all. The } microscope is a FEI Titan. } } Here is a link to a few images. One taken with the power supply switched off } and the related FFT, and one with the power supply switched on and the } related FFT. } (https://onedrive.live.com/redir?resid=507493AEB39D8528!211&authkey=!AHqWGyO } oE_yhiCw&ithint=folder%2cjpg) } } Did anybody face the same or has an idea about it? } Any help is very much appreciated. } } Thanks a lot } Jens } } } ==============================Original Headers============================== } 7, 24 -- From jens.kling-at-web.de Mon Mar 9 07:23:17 2015 } 7, 24 -- Received: from mout.web.de (mout.web.de [212.227.15.3]) } 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t29CNHEZ001516 } 7, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2015 07:23:17 -0500 } 7, 24 -- Received: from CENJens ([130.225.78.50]) by smtp.web.de (mrweb004) with } 7, 24 -- ESMTPSA (Nemesis) id 0MNx4F-1YOcFr1pcZ-007XcV for } 7, 24 -- {Microscopy-at-microscopy.com} ; Mon, 09 Mar 2015 13:23:16 +0100 } 7, 24 -- From: "Jens Kling" {jens.kling-at-web.de} } 7, 24 -- To: {Microscopy-at-microscopy.com} } 7, 24 -- Subject: TEM Gatan heating holder issue } 7, 24 -- Date: Mon, 9 Mar 2015 13:23:15 +0100 } 7, 24 -- Message-ID: {001201d05a63$d1dd7c80$75987580$-at-web.de} } 7, 24 -- MIME-Version: 1.0 } 7, 24 -- Content-Type: text/plain; } 7, 24 -- charset="us-ascii" } 7, 24 -- Content-Transfer-Encoding: 7bit } 7, 24 -- X-Mailer: Microsoft Outlook 14.0 } 7, 24 -- Thread-Index: AdBaY3QbnkVpQNnKRI+AAy79f8sH+Q== } 7, 24 -- Content-Language: en-us } 7, 24 -- X-Provags-ID: V03:K0:bMFxPjQpRAB5zt0uiHriJv86s82zFbA8i5W6/kLldFnvuaqDi0S } 7, 24 -- 1pvu600FEkpnz0mVvC2kWuqdO/Nv9qpwNXfLKYEnPmV3ymQ5/lg/9NXE9+XHVGXu1hNOwa1 } 7, 24 -- nG2IpsHHuNjWILMYtGTPOaeum6RX1wwzMWdZKdkI8GWOaZkiONQ8hI67Lv5vEepEKCdWNmK } 7, 24 -- euL3rsnTI/ck31VcTR6uQ== } 7, 24 -- X-UI-Out-Filterresults: notjunk:1; } ==============================End of - Headers==============================
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Email: twh-at-cen.dtu.dk Name: Thomas Hansen
Organization: DTU
Title-Subject: [Filtered] TIA on Windows 8.1
Message: Hi.
Has anyone managed to get the TIA software from FEI running on a Windows 8.1 PC?
Thanks.
Thomas
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==============================Original Headers============================== 18, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 9 08:55:53 2015 18, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t29DtrSY001518 18, 17 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2015 08:55:53 -0500 18, 17 -- Message-ID: {54FDA669.7010301-at-microscopy.com} 18, 17 -- Date: Mon, 09 Mar 2015 08:55:53 -0500 18, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 18, 17 -- MIME-Version: 1.0 18, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 17 -- Subject: viaWWW:FEI TIA on Windows 8.1 18, 17 -- References: {201503091103.t29B3tHO026729-at-ns.microscopy.com} 18, 17 -- In-Reply-To: {201503091103.t29B3tHO026729-at-ns.microscopy.com} 18, 17 -- X-Forwarded-Message-Id: {201503091103.t29B3tHO026729-at-ns.microscopy.com} 18, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The only way I was able to get TIA running was to set up a virtual machine and install WinXP in it. I used VirtualBox though there are other virtual machine programs as well.
Good luck, Henk
On 3/9/2015 9:57 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from:twh-at-cen.dtu.dk } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy bothtwh-at-cen.dtu.dk as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email:twh-at-cen.dtu.dk } Name: Thomas Hansen } } Organization: DTU } } Title-Subject: [Filtered] TIA on Windows 8.1 } } Message: Hi. } } Has anyone managed to get the TIA software from FEI running on a Windows 8.1 PC? } } Thanks. } } Thomas } } } Login Host: 130.225.78.31 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } }
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Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
The deadline for abstract submission has passed and as you are making plans to attend the Portland meetings (Aug. 2-6), please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.
The students will be paid $10 an hour to work for ~20 hours (up to 40 hours) during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia (assisting with audio-visual needs, speaker set-up, maintaining an attendance count), staffing the volunteer office, monitoring use of the Internet Café, and helping with vendor tutorial sign-up. Payment is given as a check at the end of the meetings or when the student leaves Portland.
Once the program has been finalized, each registered bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up "working" sessions they would attend anyway. There is an added bonus of $10 cash to help with meals for each morning and/or afternoon session worked and a meeting shirt.
If anyone would like to participate in the bursary program, please check the "I wish to apply for a student bursary" box in section 2 of the registration form. Also, please send me an email of your intention. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.
For those 'non-students' volunteers are also needed to help with the above mentioned meeting activities. Although not paid on an hourly basis as the student bursaries, volunteers do receive a meeting shirt and the same cash allotment to help with meals. Volunteers also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact:
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-7998 alawrence-at-i2at.msstate.edu
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I'm trying to export data from a 2D EELS spectrum image in Digital Micrograph and Išm not sure what the best approach is. Here is my problem:
1. I have a 2D EELS map / SI of a thin film interface, where x is some width parallel to the interface, y is some width perpendicular to the interface, and z is the EELS energy range (400 700 eV). 2. I would like to integrate all the spectra in plane (x-direction) to improve signal-to-noise (This would essentially leave me with an EELS line scan parallel to y). 3. I would then like to export slices at specified integration windows normal to the plane (y-direction) to text files.
The only way I can currently do this is by drawing an ROI onto the SI, which generates an individual spectrum integrated across x. I then have to export this and drag the ROI, repeating ad nauseam until the entire y length of the scan is traversed. Is there a simpler and faster way to do this?
Please let me know if you need more clarification. Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
Hi Steven, If you use MATLAB you can import DM3 files. Here's what might be a useful link (I have not used this particular one but it looks simple): http://www.mathworks.com/matlabcentral/fileexchange/29351-dm3-import-for-gat an-digital-micrograph
ImageJ will also open .DM3 files directly; I do all my analyses in that package. There are plugins that can be used for this as well, that you can try on spectrum images.
Once you are in one of those programs you can easily write scripts to do any arbitrary operation on the data.
Good Luck! -Larry Scipioni
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: Monday, March 09, 2015 8:17 PM To: LES-at-ZSGENETICS.COM
Hello everyone,
I'm trying to export data from a 2D EELS spectrum image in Digital Micrograph and Išm not sure what the best approach is. Here is my problem:
1. I have a 2D EELS map / SI of a thin film interface, where x is some width parallel to the interface, y is some width perpendicular to the interface, and z is the EELS energy range (400 700 eV). 2. I would like to integrate all the spectra in plane (x-direction) to improve signal-to-noise (This would essentially leave me with an EELS line scan parallel to y). 3. I would then like to export slices at specified integration windows normal to the plane (y-direction) to text files.
The only way I can currently do this is by drawing an ROI onto the SI, which generates an individual spectrum integrated across x. I then have to export this and drag the ROI, repeating ad nauseam until the entire y length of the scan is traversed. Is there a simpler and faster way to do this?
Please let me know if you need more clarification. Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
I would suggest that you use a multivariate statistical analysis (MSA) approach such as using AXSIA (Automated eXpert Spectrum Image Analysis) software or the MSA plug-in for DM that does Principal Component Analysis. The AXSIA software uses Matlab and the SIMSIMAN module that comes with the AXSIA software would allow you to extract your line profile easily. It would also be available in Matlab for any manipulation or export that you need. The MSA plug-in would allow you to reconstruct your data to improve your signal to noise for the profile. In both cases, you must be careful to align your EELS spectra in energy throughout the spectrum image using either the zero loss peak or a peak that is in both phases and does not have a chemical shift. You must also take care of eliminating X-ray peaks in your EELS data, otherwise they are identified as unique phases. If you do this, you minimize the number of factors (components) identified. Masasha Watanabe and Paul Kotula gave excellent tutorial talks at M&M'13 on the topic. Both are available online for viewing. You might have to contact John Mansfield for the link because I don't have it available as I write this. The advantage of the MSA approach is that your analysis gives you an image and so any inhomogeneities across your interface in terms of the concentration profile would easily be identified. It is also a totally unbiased analysis approach.
Masashi is the author of the MSA plugin for DM and it is available through HREM Research. Paul is a co-author of the AXSIA software and a co-patent holder for it as well. I would highly recommend that you look up their publications on the topic as they are very good reference articles to have.
We just started using the AXSIA technique after having Paul Kotula give us a tutorial at the Army Research Laboratory and are finding it a very powerful. It's a bit more trickier with EELS that with XEDS, though.
-Scott Walck
----- Original Message ----- X-from: "steven spurgeon" {steven.spurgeon-at-pnnl.gov} To: "S Walck" {S.Walck-at-comcast.net} Sent: Monday, March 9, 2015 8:17:02 PM
Hello everyone,
I'm trying to export data from a 2D EELS spectrum image in Digital Micrograph and I�m not sure what the best approach is. Here is my problem:
1. I have a 2D EELS map / SI of a thin film interface, where x is some width parallel to the interface, y is some width perpendicular to the interface, and z is the EELS energy range (400 ďż˝ 700 eV). 2. I would like to integrate all the spectra in plane (x-direction) to improve signal-to-noise (This would essentially leave me with an EELS line scan parallel to y). 3. I would then like to export slices at specified integration windows normal to the plane (y-direction) to text files.
The only way I can currently do this is by drawing an ROI onto the SI, which generates an individual spectrum integrated across x. I then have to export this and drag the ROI, repeating ad nauseam until the entire y length of the scan is traversed. Is there a simpler and faster way to do this?
Please let me know if you need more clarification. Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
Steven, Digital Micrograph has a host of tools for dealing with 3-D data sets. There is a menu labeled "Volume". This will allow you to rotate or project the data along any direction needed.
For your application, you would want to project the data along the "y". You will now have a 2D dataset with the projected intensity along the interface in the X-Dimension and Energy in the Y-Dimension.
To save this as a series of files, you would then use the "File:Save As Series ..." menu item. You can choose EMSA format for the file type and the EELS header information and calibrations will be preserved. You can also use the "Text" format, then you only get the intensities.
You can write a simple script in Digital Micrograph to do this. Below is an example. It took about 4x longer to document that actually write.
For more information about scripting, there is a good reference section in the Digital Micrograph help file. You can also get a lot of information at the DM Script site at TUGraz { http://portal.tugraz.at/portal/page/portal/felmi/DM-Script }
Hope this helps, Ray
//**************** // Simple script to project a Spectrum Image into a Line Scan.
image imgSrc := GetFrontImage() // Grabs a pointer to the front dataset. This is your 3D Spectrum Image image imgRes; // Variable for result. This does not yet exist
// Get the size of the spectrum image number sX, sY, sZ; sX = imgSrc.ImageGetDimensionSize(0); sY = imgSrc.ImageGetDimensionSize(1); sZ = imgSrc.ImageGetDimensionSize(2);
// Choose a projection direction. Returns "1" for "Along X" and "0" for "Along Y". // 1/0 is interpreted as true/false (Actually 0 = false, non-zero = true) number projectX = TwoButtonDialog("Choose a projection direction", "Along X", "Along Y")
//Create the image to receive the projection // Uses the "ImageClone" function to keep all of the acquisition tags // Uses the "Slice2" function to choose a sub-region of the original data // There are more elegant ways to do this, but this is simple. if(projectX) imgRes := ImageClone(imgSrc.Slice2(0, 0, 0, 2, sZ, 1 , 1, sY, 1)); else imgRes := ImageClone(imgSrc.Slice2(0, 0, 0, 2, sZ, 1 , 0, sX, 1));
// Set the new image to all zeros. imgRes = 0;
//Do the projection. Uses the image iterators to operate on the entire image at once. This could be done // with a for loop, but with an interpreted language, it would take forever. // This is compiled on the fly and very fast.
// Sets some image information. Uses the simple "If, Then ,Else" structure ( "logical statement" ? "Do if true" : "Do if false") // You can use this structure to operate of every pixel in an image. imgRes.SetNumberNote("EELS:Acquisition:Number of frames", (projectX ? sX : sY)) imgRes.SetName(imgSrc.GetName() + "_Projection") imgRes.ShowImage() // If you do not show the image, it is killed when the script exits.
//********************
Ray D. Twesten, Ph.D. Product Manager Analytical Instruments Gatan, Inc. Tel. +1 (925) 224-7392
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: Monday, March 09, 2015 5:08 PM To: ray.twesten-at-sbcglobal.net
Hello everyone,
I'm trying to export data from a 2D EELS spectrum image in Digital Micrograph and Išm not sure what the best approach is. Here is my problem:
1. I have a 2D EELS map / SI of a thin film interface, where x is some width parallel to the interface, y is some width perpendicular to the interface, and z is the EELS energy range (400 700 eV). 2. I would like to integrate all the spectra in plane (x-direction) to improve signal-to-noise (This would essentially leave me with an EELS line scan parallel to y). 3. I would then like to export slices at specified integration windows normal to the plane (y-direction) to text files.
The only way I can currently do this is by drawing an ROI onto the SI, which generates an individual spectrum integrated across x. I then have to export this and drag the ROI, repeating ad nauseam until the entire y length of the scan is traversed. Is there a simpler and faster way to do this?
Please let me know if you need more clarification. Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
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Email: lauri.rimorin-at-nih.gov Name: Lauri Rimorin
Organization: Leidos Biomedical Research, Inc.
Title-Subject: [Filtered] Scientist I Opening in Frederick, MD (FNLCR)
Message: Leidos Biomedical Research, Inc. (LBRI), a wholly owned subsidiary of Leidos, operates the Frederick National Laboratory for Cancer Research (FNLCR). FNLCR is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI). It is the only FFRDC dedicated to biomedical research. Through its status as an FFRDC, FNLCR provides NCI and others with a unique national resource to accelerate the development and delivery of effective preventive, diagnostic, and therapeutic products for cancer and AIDS.
The breadth of FNLCRÂs activities spans the research and development spectrum, including investigator-initiated, hypothesis-driven research into cancer and AIDS; advanced technology programs focused on genetics and genomics, proteins and proteomics, imaging, nanotechnology, bioinformatics, and laboratory animal sciences; clinical operations in support of NCI and National Institute of Allergy and Infectious Diseases (NIAID)-sponsored clinical trials, as well as NCI drug discovery and development efforts; and management and operations of biopharmaceutical development and manufacturing programs under current Good Manufacturing Practice conditions for NCI and NIAID. Administrative, procurement, financial, safety, and facilities support is provided to these R&D activities through state-of-the-art business processes. LBRI has approximately 1,900 employees and manages an annual operating budget of approximately $450M.
For more information about Leidos Biomedical Research Inc., please visit our webpage at www.leidosbiomed.com.
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The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community. A major focus of the CRTP is the NCI RAS Initiative with the goal to discover new therapeutic interventions against RAS-driven cancers. In addition, the CRTP hosts the Nanotechnology Characterization Laboratory (NCL) which performs and standardizes preclinical efficacy and toxicity testing of nanoparticles intended for cancer therapeutics and diagnostics, and the Antibody Characterization Lab (ACL) which characterizes monoclonal antibodies or other renewable affinity binding reagents for use in cancer related research. CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.
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The Scientist I will perform: 1) negative staining and cryo-EM of proteins and protein complexes with biological sample preparation and imaging by traditional transmission electron microscopy (TEM) and scanning electron microscopy (SEM), 2) image analysis and three-dimensional reconstructions on data from negative stained specimens, cryo-electron microscopy, and electron tomography, 3) sample preparation for electron microscopy studies (negative staining, SEM, room temperature plastic embedding, cryo-electron microscopy), 4) operation of electron microscopes (SEM and TEM) and cryogenic instrumentation, 5) image analysis and three dimensional reconstructions of electron microscopy data, and 6) segmentation, interpretation and presentation of two-dimensional averages and three-dimensional volume data and fitting of high resolution data into electron density maps.
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ÂPossession of Doctoral degree from an accredited college or university in a field related to Computational Science, Physics, Biology or Chemistry, or eight (8) years related experience in lieu of degree ÂForeign degrees must be evaluated for U.S. equivalency ÂProficiency in biological and/or material sample preparation for electron microscopy ÂProficiency in the use and maintenance of transmission electron microscopes and scanning electron microscopes ÂStrong analytical skills and ability to effectively communicate scientific results ÂExperience with computational image analysis of electron microscopy data (e.g. Relion, Eman, Spider, Imagic, or similar)
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ÂExperience with scripting or other programming skills ÂExperience with cryogenic liquids ÂExperience with maintenance of advanced instrumentation ÂExperience with single particle reconstruction from negative stain and/or cryo-EM data
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When: April 27-28, 2015 Where: Norwood, Massachusetts What: Workshop on Atomic Force Microscopy to Characterize Polymers
Led by Dalia Yablon, Ph.D., this two day course mixes lecture with labwork on the basics of atomic force microscopy and its specific application to imaging polymer materials.
AFM hardware and software will be reviewed, with special emphasis on the imaging modes and image processing needed to study polymer materials.
While we utilize AFMs from AFMWorkshop to teach basic concepts and demonstrate AFM operation, attendees with experience on any make or model of atomic force microscope will find the labwork relevant and practical.
All levels of experience are welcome. An "early bird" discount registration is being offered through March, 2015.
For more information, please visit: http://www.afmworkshop.com/characterization-of-polymer-materials-afm-training.html
Thank you, Pamela Stone
Pamela Stone AFMWorkshop, Inc. 1434 E. 33rd Street Signal Hill, CA 90755 www.afmworkshop.com
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Organization: Instituto Gulbenkian de Cięncia, Portugal
Title-Subject: [Filtered] TEM - Hair Samples
Message: Dear List,
We are trying to preserve newborn hair samples by HPF / AFS for TEM analysis. We are having the problem that we can not section the hair samples because the sample keeps popping out of the Epon blocks. If anyone has any experience processing hair samples, could you please contact me? I would appreciate learning how you did your infiltration, what resin you used and if you have any tricks to overcoming the problem of small samples popping out of blocks.
Thank-you for your help Erin
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Mar 12 18:23:21 2015 15, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2CNNKHf015639 15, 17 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2015 18:23:21 -0500 15, 17 -- Message-ID: {55021FE9.3010505-at-microscopy.com} 15, 17 -- Date: Thu, 12 Mar 2015 18:23:21 -0500 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:TEM - Hair Samples 15, 17 -- References: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hair and other keratin fibres are not easy tissues and must be treated differently than normal tissue samples. However, what method you need depends on if you are looking at the hair above the skin only, or if you are also looking at the hair follicle. For the moment I'll assume you are interested in only the hair above the skin.
Hair is already fixed by nature. It is also very dense. The water content is very low, and the cells are dead. It is the opposite to normal living cells in terms of the problems for TEM preparation. It can be very easy to work with also depending on what features you want to see.
The easiest method is to wash the hair to remove external lipids and dirt, place the hairs across a small frame made of plastic, or thread hairs through a narrow plastic tube, embed in LR-White (epoxy is ok too), trim, section with a diamond knife to about 100 nm/gold sections and section stain with uranyl acetate and lead citrate (slightly extended stain times compared to normal) and you can see most features.
The resin will not penetrate the hair. But the hair will sit inside the resin. There are often problems with folding (you can reduce this with thicker sections) and sometimes problems at the edges of the fibres where the fibre has swollen with the water in the knife boat while the resin has not.
If you want to see the intermediate filaments that make up most of the cortex of the hair, you have to do something more complicated involving repeated treatments of reduction to open up disulfides to attach stain to and use osmium. Or there is also a silver nitrate method which allows you to see the filaments, but at the expense of seeing various other structures.
I'll send a separate email to you with a paper that colleagues and I put together with all these methods.
Harland, D. P., Vernon, J. A., Walls, R. J., & Woods, J. L. (2011). Transmission electron microscopy staining methods for the cortex of human hair: a modified osmium method and comparison with other stains. Journal of Microscopy, 243(2), 184-196. doi: 10.1111/j.1365-2818.2011.03493.x
Kind regards Duane
____________________________ Dr Duane P Harland Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz AgResearch Limited Lincoln Research Centre Cnr Springs Rd & Gerald Street, Lincoln Private Bag 4749 Christchurch 8140, New Zealand T +64 3 325 9900 F +64 3 325 9946 www.agresearch.co.nz Farming Food and Health. First Te Ahuwhenua Te Kai me te Whai Ora. Tuatahi
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Organization: Instituto Gulbenkian de Cięncia, Portugal
Title-Subject: [Filtered] TEM - Hair Samples
Message: Dear List,
We are trying to preserve newborn hair samples by HPF / AFS for TEM analysis. We are having the problem that we can not section the hair samples because the sample keeps popping out of the Epon blocks. If anyone has any experience processing hair samples, could you please contact me? I would appreciate learning how you did your infiltration, what resin you used and if you have any tricks to overcoming the problem of small samples popping out of blocks.
Thank-you for your help Erin
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Mar 12 18:23:21 2015 15, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2CNNKHf015639 15, 17 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2015 18:23:21 -0500 15, 17 -- Message-ID: {55021FE9.3010505-at-microscopy.com} 15, 17 -- Date: Thu, 12 Mar 2015 18:23:21 -0500 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:TEM - Hair Samples 15, 17 -- References: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers============================== ======================================================================= Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately. =======================================================================
Wešve recently been conducting STEM-EELS analysis of chromium oxides and wešd like to get more precise about our quantification of white line ratios in these compounds. To that end, wešd like to prepare Cr standards that are not oxides (to avoid overlap between the Cr L23 and O K edges). Does anyone have any recommendations for compounds that have worked well for this purpose? I am currently considering buying powders such as CrCl3, but Išd be open to suggestions and preparation tips.
Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
we are trying to observe ice in the SEM. The purpose is visualising the transition of high density ice (ice II or III) to low density ice, either ice Ic or Ih using EBSD. The cryo stage in the present setup does not get lower than -140°C and this is only just below the recrystallisation temperature.
To prevent charging gas is admitted into the microscope. This however has a tremendous effect on the stage temperature which easily goes up to -110°C, way above the recrystallisation temperature. As a result we have so far of course not been able to identify the high pressure ice polymorphs.
The best remedy would very probably be to improve the cold stage so we can reach lower temperatures, and for the future this may well be what will be done. For the time being I am looking for alternatives to admitting gas to prevent charging.
Two ideas popped up: freezing salt water or freezing a conductive nanoparticle solution, e.g. gold or silver or as was suggested by Guenter Resch carbon rods. The rationale being that in the eutectica between the pure ice crystals a high concentration of ions or nanoparticles forms a network of conductive material that might or might not assist in reducing charge build up.
Does anyone of you have experience in this area or have alternative ideas?
Thanks,
Jan Leunissen Dept Geology - University of Otago Dunedin New Zealand.
==============================Original Headers============================== 9, 31 -- From leunissen-at-aurion.nl Mon Mar 16 16:00:11 2015 9, 31 -- Received: from nm13-vm6.access.bullet.mail.bf1.yahoo.com (nm13-vm6.access.bullet.mail.bf1.yahoo.com [216.109.115.6]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2GL0AP9005972 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2015 16:00:10 -0500 9, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1426539610; bh=LbOeOHymtFJyx7REZKA3iU/601T/No4Awkby3xmJ3fI=; h=From:Subject:Date:To:From:Subject; b=AFpHWSs2ePzn5fAdXWlMup/y405G67MD2oZENfXo48XwNsW/JgMNYvsJN//7s6XKNyiZnZeDFn0XTy37FB+PnP/2LINCA8TOxkXRlKpcOExMLLgteqbsSPZn2kXV6xvCS2l8RTtsvtQ9ao3QJs4ooI8xim6RfwinwFiI9HHkqy60X2I4XSFzqEEWM6SIIdjfMxukqcSNBNDRf1MCNdfuT59vEJpxRkow+i/8q5nhOX9poyXIj/T8MRsZhb0rvDxL7LpJHNJBrspIZst6gvyFqMvWj6urQ+2R8MiipRIgBG7nI8Pl67WKfM/wE9Ugeh4Y9zMKOlAyDb0qw8ocVeFXlQ== 9, 31 -- Received: from [66.196.81.158] by nm13.access.bullet.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- Received: from [98.139.244.50] by tm4.access.bullet.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- Received: from [127.0.0.1] by smtp112.sbc.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- X-Yahoo-Newman-Id: 270704.77657.bm-at-smtp112.sbc.mail.bf1.yahoo.com 9, 31 -- X-Yahoo-Newman-Property: ymail-3 9, 31 -- X-YMail-OSG: y2Aps44VM1l2nRyUVnqPdnc5H19i9IJN80qoZcPtGyQIMSH 9, 31 -- C3WjFz7v33PxOsOVonRNZUjGr4r2FjI0CpAVFxHi0LRMO1OOINquaoBPudyE 9, 31 -- snE9gGKVZBqla05z_GNToYDwWQnRDXtIH3POgFMtrzetr4_KX4xOR_C5y8s5 9, 31 -- GI80EaPaxEiuo1Joq45_VU6Ipspvt7tlJMzWVEl6sD3pSrcXtPBpGPVmjdbr 9, 31 -- iKwgaSYLp_2VP1gqkN1Zs1WoM4dCX7IYU_PJZ06vsW0UFums_yO_bfqpgIXL 9, 31 -- OoDftKCv_kJBq2UHrAgI_Ps4CK0KoqKYCHAxJuqaEZwkrmjRTwcLvRJSKQ.R 9, 31 -- iqDSVt7ACSG_YJ6I284694ok0KUPsHCQz2XFOSDJlGwiJbJNb_3ZMdZsGQrc 9, 31 -- wZNqaVz_2VbFbDrjFCjPlbjmcaJO0pmSD8LGtBErRimgzZgjV0wRJnoVWThc 9, 31 -- J7KKe82fdQeDQwyrfgD2S76Iirky.Kg2N963MYcDjnUfQbWeEY3.AvVd_7em 9, 31 -- IQ_rFoCee4yHmBj8R0Sbt9kN98CoLpOPuBJjTGaYf3dPdEA-- 9, 31 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 9, 31 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 31 -- Content-Type: text/plain; charset=utf-8 9, 31 -- Subject: charging of ice in SEM 9, 31 -- Message-Id: {EE9118FD-D201-417F-87EB-388AF90D6781-at-aurion.nl} 9, 31 -- Date: Tue, 17 Mar 2015 10:00:05 +1300 9, 31 -- To: microscopy-at-microscopy.com 9, 31 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.6\)) 9, 31 -- X-Mailer: Apple Mail (2.2070.6) 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2GL0AP9005972 ==============================End of - Headers==============================
In my past life I ran a FE-SEM with cryostage, and charging of ice was of little issue at 1-2 kV. Most frequently, 1 or 1.5 kV. What sort of instrument are you using? Can you get a low enough kV to reach charge balance?
And ... adding nanoparticles, salt water, etc. I'd wonder about that. Yes, the crystallization process does exclude ions and such to produce the ice crystals (sea ice is really interesting because of this), but I doubt that process is 100% complete. I suspect it would be less complete with nanoparticles than it is with ions. Which means adding salts or nanoparticles will affect the properties you're trying to study. Plus, the added salts/nanoparticles are going to add electrical effects to your samples, even if they are excluded from the crystals. What do these do?
Phil
} Hello, } } we are trying to observe ice in the SEM. The purpose is visualising the transition of high density ice (ice II or III) to low density ice, either ice Ic or Ih using EBSD. } The cryo stage in the present setup does not get lower than -140°C and this is only just below the recrystallisation temperature. } } To prevent charging gas is admitted into the microscope. This however has a tremendous effect on the stage temperature which easily goes up to -110°C, way above the recrystallisation temperature. As a result we have so far of course not been able to identify the high pressure ice polymorphs. } } The best remedy would very probably be to improve the cold stage so we can reach lower temperatures, and for the future this may well be what will be done. For the time being I am looking for alternatives to admitting gas to prevent charging. } } Two ideas popped up: freezing salt water or freezing a conductive nanoparticle solution, e.g. gold or silver or as was suggested by Guenter Resch carbon rods. The rationale being that in the eutectica between the pure ice crystals a high concentration of ions or nanoparticles forms a network of conductive material that might or might not assist in reducing charge build up. } } Does anyone of you have experience in this area or have alternative ideas? } } } Thanks, } } Jan Leunissen } Dept Geology - University of Otago } Dunedin } New Zealand.
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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On Mar 16, 2015, at 2:23 PM, leunissen-at-aurion.nl wrote:
} we are trying to observe ice in the SEM. The purpose is visualising } the transition of high density ice (ice II or III) to low density } ice, either ice Ic or Ih using EBSD. } The cryo stage in the present setup does not get lower than -140°C } and this is only just below the recrystallisation temperature. } } To prevent charging gas is admitted into the microscope. This } however has a tremendous effect on the stage temperature which } easily goes up to -110°C, way above the recrystallisation } temperature. As a result we have so far of course not been able to } identify the high pressure ice polymorphs. } } The best remedy would very probably be to improve the cold stage so } we can reach lower temperatures, and for the future this may well be } what will be done. For the time being I am looking for alternatives } to admitting gas to prevent charging. } } Two ideas popped up: freezing salt water or freezing a conductive } nanoparticle solution, e.g. gold or silver or as was suggested by } Guenter Resch carbon rods. The rationale being that in the eutectica } between the pure ice crystals a high concentration of ions or } nanoparticles forms a network of conductive material that might or } might not assist in reducing charge build up. } } Does anyone of you have experience in this area or have alternative } ideas? } } } Thanks, } } Jan Leunissen } Dept Geology - University of Otago } Dunedin } New Zealand.
Dear Jan, I have no experience in SEM of ice, but from other posts to this list, I would try low-voltage SEM to balance electrons staying in the specimen with secondary electrons leaving the specimen. An additional comment is that trying to freeze salt water is likely to result in crystals of ice surrounded by molecules of salt, since most salts do not dissolve in ice. One exception, which I have also considered in order to increase the conductivity of ice, is NH4F, since both NH3 and HF can incorporate into the ice structure--the reference for this is a book called Physics of Ice, the name of the author of which I do not remember. Yours, Bill
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Thank you, everyone, for your replies. A brief summary below since not all messages were cced to this list.
The idea of freezing brine or a conductive nanoparticle suspension met with scepticism since the ice will separate from the conductive components. The general recommendation was: use as low a kV as you can, it will cause less charging and the surface charge may even become positive.
Unfortunately the low kV and EBSD do not go well together.
Whereas the geology department in Dunedin has been very successful in imaging Ice Ih and getting excellent EBSD patterns from it, this can be done at much higher temperatures since recrystallisation is not an issue. The situation is more complicated for the high pressure crystalline ice polymorphs as the temperature of the ice must not be warmer than ~ -135°C.
A tough nut to crack, but an interesting one, so not giving up yet.
Thank you all, it was great to have so many replies. As you will have been able to tell from my question and the omission of essential details I am not an SEM expert, so your help was very valuable.
Jan
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } } we are trying to observe ice in the SEM. The purpose is visualising the transition of high density ice (ice II or III) to low density ice, either ice Ic or Ih using EBSD. } The cryo stage in the present setup does not get lower than -140°C and this is only just below the recrystallisation temperature. } } To prevent charging gas is admitted into the microscope. This however has a tremendous effect on the stage temperature which easily goes up to -110°C, way above the recrystallisation temperature. As a result we have so far of course not been able to identify the high pressure ice polymorphs. } } The best remedy would very probably be to improve the cold stage so we can reach lower temperatures, and for the future this may well be what will be done. For the time being I am looking for alternatives to admitting gas to prevent charging. } } Two ideas popped up: freezing salt water or freezing a conductive nanoparticle solution, e.g. gold or silver or as was suggested by Guenter Resch carbon rods. The rationale being that in the eutectica between the pure ice crystals a high concentration of ions or nanoparticles forms a network of conductive material that might or might not assist in reducing charge build up. } } Does anyone of you have experience in this area or have alternative ideas? } } } Thanks, } } Jan Leunissen } Dept Geology - University of Otago } Dunedin } New Zealand. } } ==============================Original Headers============================== } 9, 31 -- From leunissen-at-aurion.nl Mon Mar 16 16:00:11 2015 9, 31 -- Received: from nm13-vm6.access.bullet.mail.bf1.yahoo.com (nm13-vm6.access.bullet.mail.bf1.yahoo.com [216.109.115.6]) } 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2GL0AP9005972 } 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2015 16:00:10 -0500 } 9, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1426539610; bh=LbOeOHymtFJyx7REZKA3iU/601T/No4Awkby3xmJ3fI=; h=From:Subject:Date:To:From:Subject; b=AFpHWSs2ePzn5fAdXWlMup/y405G67MD2oZENfXo48XwNsW/JgMNYvsJN//7s6XKNyiZnZeDFn0XTy37FB+PnP/2LINCA8TOxkXRlKpcOExMLLgteqbsSPZn2kXV6xvCS2l8RTtsvtQ9ao3QJs4ooI8xim6RfwinwFiI9HHkqy60X2I4XSFzqEEWM6SIIdjfMxukqcSNBNDRf1MCNdfuT59vEJpxRkow+i/8q5nhOX9poyXIj/T8MRsZhb0rvDxL7LpJHNJBrspIZst6gvyFqMvWj6urQ+2R8MiipRIgBG7nI8Pl67WKfM/wE9Ugeh4Y9zMKOlAyDb0qw8ocVeFXlQ== } 9, 31 -- Received: from [66.196.81.158] by nm13.access.bullet.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- Received: from [98.139.244.50] by tm4.access.bullet.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- Received: from [127.0.0.1] by smtp112.sbc.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- X-Yahoo-Newman-Id: 270704.77657.bm-at-smtp112.sbc.mail.bf1.yahoo.com } 9, 31 -- X-Yahoo-Newman-Property: ymail-3 9, 31 -- X-YMail-OSG: y2Aps44VM1l2nRyUVnqPdnc5H19i9IJN80qoZcPtGyQIMSH } 9, 31 -- C3WjFz7v33PxOsOVonRNZUjGr4r2FjI0CpAVFxHi0LRMO1OOINquaoBPudyE } 9, 31 -- snE9gGKVZBqla05z_GNToYDwWQnRDXtIH3POgFMtrzetr4_KX4xOR_C5y8s5 } 9, 31 -- GI80EaPaxEiuo1Joq45_VU6Ipspvt7tlJMzWVEl6sD3pSrcXtPBpGPVmjdbr } 9, 31 -- iKwgaSYLp_2VP1gqkN1Zs1WoM4dCX7IYU_PJZ06vsW0UFums_yO_bfqpgIXL } 9, 31 -- OoDftKCv_kJBq2UHrAgI_Ps4CK0KoqKYCHAxJuqaEZwkrmjRTwcLvRJSKQ.R } 9, 31 -- iqDSVt7ACSG_YJ6I284694ok0KUPsHCQz2XFOSDJlGwiJbJNb_3ZMdZsGQrc } 9, 31 -- wZNqaVz_2VbFbDrjFCjPlbjmcaJO0pmSD8LGtBErRimgzZgjV0wRJnoVWThc } 9, 31 -- J7KKe82fdQeDQwyrfgD2S76Iirky.Kg2N963MYcDjnUfQbWeEY3.AvVd_7em } 9, 31 -- IQ_rFoCee4yHmBj8R0Sbt9kN98CoLpOPuBJjTGaYf3dPdEA-- } 9, 31 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF } 9, 31 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 31 -- Content-Type: text/plain; charset=utf-8 9, 31 -- Subject: charging of ice in SEM 9, 31 -- Message-Id: {EE9118FD-D201-417F-87EB-388AF90D6781-at-aurion.nl} } 9, 31 -- Date: Tue, 17 Mar 2015 10:00:05 +1300 9, 31 -- To: microscopy-at-microscopy.com 9, 31 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.6\)) 9, 31 -- X-Mailer: Apple Mail (2.2070.6) 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2GL0AP9005972 ==============================End of - Headers==============================
==============================Original Headers============================== 12, 37 -- From leunissen-at-aurion.nl Thu Mar 19 16:32:39 2015 12, 37 -- Received: from nm23-vm7.access.bullet.mail.bf1.yahoo.com (nm23-vm7.access.bullet.mail.bf1.yahoo.com [216.109.115.166]) 12, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2JLWchw025102 12, 37 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2015 16:32:39 -0500 12, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1426800758; bh=MTIclw8O86ckCCe93kIC29b8SYz64cAwrTQGl61/vCc=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=QgQKAssfmm3owKDJkIS14TobjlYF9eYfQ5RArDpi5hbcTRvzly1z4DxufKqqaZBpzceKrup+HkwN+ht9GKcgXi1/xWJt2un5A/PyomkWMKLWjtZ/bLEZA/MBPf2aFiEtmRIpxD3M2CN7QsTXlImygfjJLAVEzRovDomaewvwqZUVjtL7TtiOeNhhJiisSy2J7uMBaEe9382qdNclhp8BuP6osirpipNIyvOCeGDdUKBZgn6kZJZ16LfcAiYcmPG/8LaRDDJy0qt3N/8DLoIQts4QTKiHkpkP5kogHExsjpExqSB58J2HBBmLlrycLtn9LxfTo29ckYnGPbm5eYYfSw== 12, 37 -- Received: from [66.196.81.160] by nm23.access.bullet.mail.bf1.yahoo.com with NNFMP; 19 Mar 2015 21:32:38 -0000 12, 37 -- Received: from [98.139.244.51] by tm6.access.bullet.mail.bf1.yahoo.com with NNFMP; 19 Mar 2015 21:32:38 -0000 12, 37 -- Received: from [127.0.0.1] by smtp113.sbc.mail.bf1.yahoo.com with NNFMP; 19 Mar 2015 21:32:38 -0000 12, 37 -- X-Yahoo-Newman-Id: 122782.74263.bm-at-smtp113.sbc.mail.bf1.yahoo.com 12, 37 -- X-Yahoo-Newman-Property: ymail-3 12, 37 -- X-YMail-OSG: 7N1n0wQVM1n4xuLejYbY6jQasOduj6qFmHbUheGShit1dtD 12, 37 -- brN4d_bB3hAKK2ODg8cf2PIvQzt6n6wvs4xtowAxKo5QOKCGsmNQihYp1.N_ 12, 37 -- 3QT6DNNY9qm1M17ZNWtjj8OHFK1fRh0shNFMTdcYzs8XOgTh_Uj0dGWTu3zV 12, 37 -- eZnTTwJGU.9Sb_Wh7Q.Lw7_6.ca.t7L9PPSA6ARi46lXFP.e2tjARDUFRi3r 12, 37 -- e.evHcw0hT6swDU3yMgqzeRazLKc5V_dfFAZ4SNC9BJzT45H4Bc7_Kc99.2q 12, 37 -- RFWmP9c48gbCGElWDClDQ0BbJg7CeDSkVonms3tVjd_fjwVACBvxaoVybYT5 12, 37 -- DJU2yUNSrFrnsUBoBcIc6Vk0SwRU_v33r3IoIRlGhsEtS7glZHttBamV6.jJ 12, 37 -- pjjid2Z0ufnM0116K9stWtwSRQSrZ0FjhaztI48zQ9DAKrWQc5UadJiNjOiU 12, 37 -- HB_Mr3ZcVdLzWRX8Ayutty7fdCtX0U0dvXZP0vdndBNdewuX5cBfwNCkJEQe 12, 37 -- lFyG_WjT1uQ0pJns3.MhfgalLZQuXBGv7M3T4Cxxr8P9krtfgHl8vW8Bhkwy 12, 37 -- dujOnpE0YhRgj5BSFRD.LTC6KkjVYcauh85xhKd15HQ70kDg8KaaW4a4IKX8 12, 37 -- 3dUL9wPLGM2z0g277Qp3fnJPv_OXNtOIYUVfg9y0UtOhjmSJ7AWbDjYtPHFI 12, 37 -- lbh5VNFox_DPJuO0AUQGIrCM7iyvzfUU5k1elPP2XkgHC_RV8UgFUvHnqCXg 12, 37 -- 5fde_HUHIdoqEsE8eD79eMaO_dnm0F_sEzgakHxEifdME 12, 37 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 12, 37 -- Content-Type: text/plain; charset=windows-1252 12, 37 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.6\)) 12, 37 -- Subject: Re: [Microscopy] charging of ice in SEM - summary 12, 37 -- From: Jan Leunissen {leunissen-at-aurion.nl} 12, 37 -- In-Reply-To: {BLUPR04MB546B98149BE3D6CD680A10DD7030-at-BLUPR04MB546.namprd04.prod.outlook.com} 12, 37 -- Date: Fri, 20 Mar 2015 10:32:27 +1300 12, 37 -- Message-Id: {43497D60-7FDA-490C-8F31-607FDEDA75AA-at-aurion.nl} 12, 37 -- References: {201503162101.t2GL1eBs007492-at-ns.microscopy.com} {BLUPR04MB546B98149BE3D6CD680A10DD7030-at-BLUPR04MB546.namprd04.prod.outlook.com} 12, 37 -- To: microscopy-at-microscopy.com 12, 37 -- X-Mailer: Apple Mail (2.2070.6) 12, 37 -- Content-Transfer-Encoding: 8bit 12, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2JLWchw025102 ==============================End of - Headers==============================
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Email: m.aindow-at-uconn.edu Name: Mark Aindow
Organization: University of Connecticut
Title-Subject: [Filtered] Senior Staff Position: UConn-FEI Center of Excellence in Microscopy.
Message: Academic Assistant 4 - Institute of Materials Science
The Institute of Materials Science (IMS) at the University of Connecticut is seeking qualified applicants to fill the position of Academic Assistant IV for the newly established UConn-FEI Center of Excellence in Microscopy. The Center has been formed as a partnership between UConn and FEI Inc. to serve the analytical needs of industrial and academic researchers. The Center is acquiring seven new electron beam instruments over the next two years including state-of-the-art TEM, SEM and FIB systems. These will be housed in the UConn Technology Park as part of purpose-built Advanced Characterization Laboratory (ACL), which is scheduled to open in 2017.
Working under the direction of the Center Director, the Academic Assistant will co-ordinate, oversee, and be responsible for the effective and safe management of the existing IMS Microscopy Laboratory and the new Center of Excellence. Duties will include: supervising the staff and students who work for the Center; keeping abreast of published, scientific literature to understand what has been done before and any new developments in the field; sharing their knowledge with staff and students; publishing papers as appropriate; overseeing the operation, maintenance and user training on the instruments; managing systems for user booking and logging of instrument usage; responsible for maintenance of instruments and providing advice on instrument upgrades, including the co-ordination of new equipment installation in the IMS Microscopy Laboratory and the subsequent relocation to the ACL.
Minimum Qualifications: An earned doctorate in Materials Science, Chemistry, Physics or a related discipline; a minimum of five years post-doctoral experience involving the use of electron microscopes and related instrumentation; good written and verbal communication skills; strong interpersonal skills including the ability to interact effectively with faculty, staff, students and customers from industry.
Preferred Qualifications: Experience with FEI instruments, including: aberration-corrected TEMs, small dual-beam FIB systems and SEMs; Prior experience of microscopy user facility management; Strong track record of working with industry.
Appointment Terms: This is a full-time, 11-month position with an anticipated start date of May 1st. Salary will be commensurate with qualifications and experience.
To Apply: Interested applicants should submit a cover letter, C.V., and contact information for three references online at Husky Hire (www.jobs.uconn.edu). Review of applications will begin immediately. Position inquiries should be directed to the Institute Director, Dr. Steven Suib (steven.suib-at-uconn.edu). Employment of the successful candidate will be contingent upon the successful completion of a pre-employment background check. (Search # 2015336)
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If you have been considering sending in an application to NIH/National Heart, Lung and Blood Institute for an "Electron Microscopy Senior Scientist and/or Core Director" (see MSA Job posting ID 22114004) you only have about two more weeks to get it in.
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Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491 connellyps-at-mail.nih.gov
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Email: chrisbrantner-at-email.gwu.edu Name: Chris Brantner
Organization: George Washington University
Title-Subject: [Filtered] reticle for Leica Ultracut R
Message: Good afternoon listers,
I was wondering if anyone out there has a reticle that fits into the eye piece of a Leica Ultracut R that they are not using and would be willing to part with. I would like to put it on this ultramicrotome that I will be using to train students so that they can "see" how large their resin blackface is.
Thanks Chris
George Washington U-Center for Nanofabrication and Imaging Washington DC chrisbrantner-at-gwu.edu
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Email: klaus.leifer-at-angstrom.uu.se Name: Klaus Leifer
Organization: Uppsala University
Title-Subject: [Filtered] 2nd international workshop on TEM spectroscopy in materials science
Message: Uppsala University organises the 2nd international workshop on TEM spectroscopy in materials science in Uppsala (18th-20th May). We have invited very good colleagues from the field of spectroscopy and have made strong efforts to keep the registration fees very low. We believe that this could make the workshop interesting for some of your co-workers or students. Uppsala is the closest city to Stockholm airport (18min) and the airport is easy to reach for national and international flights.
Detailed Information about the workshop can be found at this URL
http://www.teknik.uu.se/elmin/spectroscopy.php
Best regards Klaus
-- Prof. Klaus Leifer, Chair of Experimental Physics, Laboratory of Electron Microscopy and Nanoengineering, Div. Applied Materials Science, Dep. Engineering Science, Uppsala University, Angstromlaboratory, Lägerhyddsv. 1, Box 534, 751 21 UPPSALA, Sweden Tel: 0046 18-471 7942, Mobile: 0046 70 425 09 81, Fax: 0046 18-471 32 70, email: klaus.leifer-at-Angstrom.uu.se
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==============================Original Headers============================== 25, 18 -- From microscopylistserver-noreply-at-microscopy.com Wed Mar 25 08:50:43 2015 25, 18 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 25, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2PDohje024544 25, 18 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2015 08:50:43 -0500 25, 18 -- Message-ID: {5512BD33.7020306-at-microscopy.com} 25, 18 -- Date: Wed, 25 Mar 2015 08:50:43 -0500 25, 18 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 25, 18 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 25, 18 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 25, 18 -- MIME-Version: 1.0 25, 18 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 25, 18 -- Subject: viaWWW:2nd international workshop on =?windows-1252?Q?=94TEM_s?= 25, 18 -- =?windows-1252?Q?pectroscopy_in_materials_science=94?= 25, 18 -- References: {201503251347.t2PDl7oX024471-at-ns.microscopy.com} 25, 18 -- In-Reply-To: {201503251347.t2PDl7oX024471-at-ns.microscopy.com} 25, 18 -- X-Forwarded-Message-Id: {201503251347.t2PDl7oX024471-at-ns.microscopy.com} 25, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 25, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We have a very valuable sample that was wrapped in Kapton tape for analysis by synchrotron and micro-CT. The problem now is that we want to remove the tape and sticky residue to prepare the sample for FIB and TEM.
Does anyone have any recipes (chemical or otherwise) for getting the polymers off cleanly? We could bake / burn the sample (it's refractory ceramic) but we don't want to do this unless necessary.
Thanks Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Radiation Effects and Microstructural Analysis Group Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 6, 37 -- From parishcm-at-ornl.gov Fri Mar 27 09:07:13 2015 6, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2RE7AcI002059 6, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2015 09:07:13 -0500 6, 37 -- X-SG: RELAYLIST 6, 37 -- X-IronPort-AV: E=Sophos;i="5.11,479,1422939600"; 6, 37 -- d="scan'208";a="77237093" 6, 37 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 6, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 27 Mar 2015 10:07:10 -0400 6, 37 -- Received: from EXCHCS31.ornl.gov (exchcs31.ornl.gov [128.219.12.145]) 6, 37 -- (using TLSv1 with cipher AES256-SHA (256/256 bits)) 6, 37 -- (No client certificate requested) 6, 37 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 31694100145 6, 37 -- for {Microscopy-at-Microscopy.Com} ; Fri, 27 Mar 2015 10:07:10 -0400 (EDT) 6, 37 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS31.ornl.gov 6, 37 -- (128.219.12.145) with Microsoft SMTP Server (TLS) id 15.0.1044.25; Fri, 27 6, 37 -- Mar 2015 10:07:09 -0400 6, 37 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by 6, 37 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id 6, 37 -- 15.00.1044.021; Fri, 27 Mar 2015 10:07:09 -0400 6, 37 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 6, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 6, 37 -- Subject: Removing Kapton tape 6, 37 -- Thread-Topic: Removing Kapton tape 6, 37 -- Thread-Index: AdBol09xycvJFFxeSWiSWHeC5oAvjw== 6, 37 -- Date: Fri, 27 Mar 2015 14:07:08 +0000 6, 37 -- Message-ID: {8601c06cd41a4b8fb03522f5764d20fb-at-EXCHCS32.ornl.gov} 6, 37 -- Accept-Language: en-US 6, 37 -- Content-Language: en-US 6, 37 -- X-MS-Has-Attach: 6, 37 -- X-MS-TNEF-Correlator: 6, 37 -- x-ms-exchange-transport-fromentityheader: Hosted 6, 37 -- x-originating-ip: [128.219.12.132] 6, 37 -- Content-Type: text/plain; charset="us-ascii" 6, 37 -- MIME-Version: 1.0 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2RE7AcI002059 ==============================End of - Headers==============================
I had good luck removing Kapton tape residue from thin (~250um) semiconductor samples by soaking in warm (~40C, covered beaker under fume hood) acetone overnight and then gently rubbing with a Q-tip soaked in acetone on a flat piece of teflon plastic.
If you find a better approach - please share.
Valery Ray - also with NISP Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
On 3/27/2015 10:09 AM, parishcm-at-ornl.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have a very valuable sample that was wrapped in Kapton tape for analysis by synchrotron and micro-CT. The problem now is that we want to remove the tape and sticky residue to prepare the sample for FIB and TEM. } } Does anyone have any recipes (chemical or otherwise) for getting the polymers off cleanly? We could bake / burn the sample (it's refractory ceramic) but we don't want to do this unless necessary. } } Thanks } Chad } } --------------------- } Chad M. Parish, Ph.D. } Research and Development Staff Member } Radiation Effects and Microstructural Analysis Group } Materials Science and Technology Division } Oak Ridge National Laboratory } Phone: 1 865 574 0092 } Email: parishcm-at-ornl.gov } } } } ==============================Original Headers============================== } 6, 37 -- From parishcm-at-ornl.gov Fri Mar 27 09:07:13 2015 } 6, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) } 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2RE7AcI002059 } 6, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2015 09:07:13 -0500 } 6, 37 -- X-SG: RELAYLIST } 6, 37 -- X-IronPort-AV: E=Sophos;i="5.11,479,1422939600"; } 6, 37 -- d="scan'208";a="77237093" } 6, 37 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) } 6, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 27 Mar 2015 10:07:10 -0400 } 6, 37 -- Received: from EXCHCS31.ornl.gov (exchcs31.ornl.gov [128.219.12.145]) } 6, 37 -- (using TLSv1 with cipher AES256-SHA (256/256 bits)) } 6, 37 -- (No client certificate requested) } 6, 37 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 31694100145 } 6, 37 -- for {Microscopy-at-Microscopy.Com} ; Fri, 27 Mar 2015 10:07:10 -0400 (EDT) } 6, 37 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS31.ornl.gov } 6, 37 -- (128.219.12.145) with Microsoft SMTP Server (TLS) id 15.0.1044.25; Fri, 27 } 6, 37 -- Mar 2015 10:07:09 -0400 } 6, 37 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by } 6, 37 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id } 6, 37 -- 15.00.1044.021; Fri, 27 Mar 2015 10:07:09 -0400 } 6, 37 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} } 6, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} } 6, 37 -- Subject: Removing Kapton tape } 6, 37 -- Thread-Topic: Removing Kapton tape } 6, 37 -- Thread-Index: AdBol09xycvJFFxeSWiSWHeC5oAvjw== } 6, 37 -- Date: Fri, 27 Mar 2015 14:07:08 +0000 } 6, 37 -- Message-ID: {8601c06cd41a4b8fb03522f5764d20fb-at-EXCHCS32.ornl.gov} } 6, 37 -- Accept-Language: en-US } 6, 37 -- Content-Language: en-US } 6, 37 -- X-MS-Has-Attach: } 6, 37 -- X-MS-TNEF-Correlator: } 6, 37 -- x-ms-exchange-transport-fromentityheader: Hosted } 6, 37 -- x-originating-ip: [128.219.12.132] } 6, 37 -- Content-Type: text/plain; charset="us-ascii" } 6, 37 -- MIME-Version: 1.0 } 6, 37 -- Content-Transfer-Encoding: 8bit } 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2RE7AcI002059 } ==============================End of - Headers============================== }
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Email: 13qw9-at-queensu.ca Name: Qiang Wang
Organization: Queen's University
Title-Subject: [Filtered] Bruker Esprit 1.9 Problem
Message: Hi all, we just installed an Bruker Esprit 1.9 offline software. Some problems happened. The first one is when I was trying to do QMap for some existed ChemiSTEM HyperMap data, all the QMap images are just black but not with different colors as usual. The second one is when I wanted to make a new QMap method, error happened like "cliff-lorimer: wrong primary energy in standard library". So, I guess there may be some parameters need to be changed? I change the "Voltage range" to 200KV in the "Microscope information" already. So, if anyone of you know any possible reason for this, please let me know. Thank you very much!
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A friend is trying to get some instruments running but doesn't have the manual for this. I found something similar, but it would be good to have the actual user manual. Does anyone have a way to get this?
I've collected what I have so far here: http://siliconpr0n.org/wiki/doku.php?id=lecroy:6010_magic_controller
John
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Organization: West Virginia Univirsity EM Department
Title-Subject: [Filtered] Service Contracts on older equipment
Message: We have an older Leica UCT ultramicrotome that is used for research and is no longer covered under service contracts provided by Leica. Are there any companies that would provide service contracts for older equipment such as this?
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 30 17:52:46 2015 15, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2UMqkt6027635 15, 17 -- for {microscopy-at-microscopy.com} ; Mon, 30 Mar 2015 17:52:46 -0500 15, 17 -- Message-ID: {5519D3BE.4070003-at-microscopy.com} 15, 17 -- Date: Mon, 30 Mar 2015 17:52:46 -0500 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:Service Contracts on older Leica equipment 15, 17 -- References: {201503301557.t2UFvcm7017716-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201503301557.t2UFvcm7017716-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201503301557.t2UFvcm7017716-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Although the older microtomes are officially no longer covered by service contracts, you can often convince your Leica microtome specialist to service them. They may complain, but they will usually do it (in our experience, at least). We recently moved into a different building, and the microtome specialist gave all of the microtomes a service, even the oldest Ultracut (pre-Ultracut E). It will just cost a chunk of money.
However, in the USA, you likely have alternatives to Leica. They may not give you a service contract but I imagine they will service the instruments - they just need to be aware of the idiosyncracies of the different models.
good luck, cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 E rosemary.white-at-csiro.au
On 31/03/15 10:00 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
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Generally when you receive the message that you have a "wrong primary energy in standard library" it means that your spectra or map was recorded at a different energy than that used to build the Esprit library.
Go to the menu "Database", then top right open the button "Standard Library" and select "new". Accept to change the current library and fill the data for the new one: -any name you like, - elevation is the take-off angle, probably 18° or 22° for Titan or Osiris respectively, - azimuth the angle between the goniometer axis and the diode positions 45° - sample tilt that you used to record the data.
Regards
Philippe
==============================Original Headers============================== 5, 19 -- From philippe.buffat-at-epfl.ch Mon Mar 30 19:35:09 2015 5, 19 -- Received: from smtp5.epfl.ch (smtp5.epfl.ch [128.178.224.8]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2V0Z98X004303 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 30 Mar 2015 19:35:09 -0500 5, 19 -- Received: (qmail 6716 invoked by uid 107); 31 Mar 2015 00:35:06 -0000 5, 19 -- X-Virus-Scanned: ClamAV 5, 19 -- Received: from vpn-254-045.epfl.ch (HELO vpn-254-045.epfl.ch) (128.179.254.45) 5, 19 -- by mail.epfl.ch (AngelmatoPhylax SMTP proxy) with ESMTP; Tue, 31 Mar 2015 02:35:06 +0200 5, 19 -- Message-ID: {5519EBB6.4090401-at-epfl.ch} 5, 19 -- Date: Tue, 31 Mar 2015 02:35:02 +0200 5, 19 -- From: Philippe Buffat {philippe.buffat-at-epfl.ch} 5, 19 -- Reply-To: philippe.buffat-at-epfl.ch 5, 19 -- Organization: EPFL 5, 19 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy-at-microscopy.com 5, 19 -- Subject: Bruker Esprit 1.9 Problem 5, 19 -- Content-Type: text/plain; charset=utf-8; format=flowed 5, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Dear All, anybody out there who can give me the original Zeiss part number of the serial interface used to remote a Zeiss DSM 960 ? It should be something like "348331-9023-1234" Or better: Somebody out there who would like to part with this interface card?
Best regards, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
We have 3 FEI FEG SEMs in our building. A new FEI FIB, a 4 yr old FEI nanoSEM and a 15 yr old FEI XL30 SFEG
We have 2 older sputter coaters. A Polaron E5100 (1988) and a Denton Desk II. Both use mech pumps. Our Polaron is no longer working
The problem of course is the visible grain at high mags (} 50Kx) with gold, gold/palladium, etc. It doesn't matter if it's the Polaron or the Denton. Same result. Visible grain over 50Kx.
I need to justify with written rationale to our directors why we should consider buying a high res coater. Simply stating "to compliment our 3 FEG SEMs" is not enough of a reason to spend the money. They prefer I fix the Polaron.
Any ideas/feedback from those who have already crossed this bridge would be helpful.
Thank you in advance
Also, does anyone have a resource for parts for Polaron coaters circa 1988 models?
Fred Hayes Manager, AMCaT Labs CHMS, College of Eng UC Davis
==============================Original Headers============================== 12, 51 -- From fahayes-at-ucdavis.edu Tue Mar 31 21:02:02 2015 12, 51 -- Received: from smtp2.ucdavis.edu (smtp2.ucdavis.edu [128.120.32.219]) 12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t312211I009135 12, 51 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2015 21:02:02 -0500 12, 51 -- Received: from exhub.ex.ad3.ucdavis.edu (mailbag.housing.ucdavis.edu [169.237.229.112]) 12, 51 -- by smtp2.ucdavis.edu (8.14.4/8.14.5/it-oel6-mimedefang-smtp-1.9) with ESMTP id t3121mde006125 12, 51 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=OK) 12, 51 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2015 19:02:00 -0700 12, 51 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (207.46.163.184) 12, 51 -- by exhub.ex.ad3.ucdavis.edu (169.237.229.112) with Microsoft SMTP Server 12, 51 -- (TLS) id 14.3.224.2; Tue, 31 Mar 2015 19:01:58 -0700 12, 51 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com (10.242.127.155) by 12, 51 -- DM2PR0801MB651.namprd08.prod.outlook.com (10.242.127.154) with Microsoft SMTP 12, 51 -- Server (TLS) id 15.1.118.21; Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) by 12, 51 -- DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) with mapi id 12, 51 -- 15.01.0118.022; Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 12, 51 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 51 -- Subject: rationale for buying a high res sputter coater 12, 51 -- Thread-Topic: rationale for buying a high res sputter coater 12, 51 -- Thread-Index: AdBsHbeDSWYu9NZsSriicsUEbzfugg== 12, 51 -- Date: Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- Message-ID: {DM2PR0801MB652387327D70F18ECE1AC91BEF30-at-DM2PR0801MB652.namprd08.prod.outlook.com} 12, 51 -- Accept-Language: en-US 12, 51 -- Content-Language: en-US 12, 51 -- X-MS-Has-Attach: 12, 51 -- X-MS-TNEF-Correlator: 12, 51 -- x-originating-ip: [169.237.64.26] 12, 51 -- authentication-results: microscopy.com; dkim=none (message not signed) 12, 51 -- header.d=none; 12, 51 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB651; 12, 51 -- x-forefront-antispam-report: BMV:1;SFV:NSPM;SFS:(10009020)(6009001)(504964003)(102836002)(50986999)(54356999)(40100003)(86362001)(87936001)(450100001)(76576001)(2501003)(2656002)(122556002)(77156002)(92566002)(2900100001)(2351001)(229853001)(62966003)(77096005)(90282001)(33656002)(74316001)(107886001)(46102003)(110136001)(99286002)(88552001)(75432002)(89122001);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR0801MB651;H:DM2PR0801MB652.namprd08.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 12, 51 -- x-microsoft-antispam-prvs: {DM2PR0801MB651C26D2D1ADE9C9B84F910BEF30-at-DM2PR0801MB651.namprd08.prod.outlook.com} 12, 51 -- x-exchange-antispam-report-test: UriScan:; 12, 51 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5002010)(5005006);SRVR:DM2PR0801MB651;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB651; 12, 51 -- x-forefront-prvs: 053315510E 12, 51 -- Content-Type: text/plain; charset="us-ascii" 12, 51 -- MIME-Version: 1.0 12, 51 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 01 Apr 2015 02:01:56.1550 12, 51 -- (UTC) 12, 51 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 12, 51 -- X-MS-Exchange-CrossTenant-id: a8046f64-66c0-4f00-9046-c8daf92ff62b 12, 51 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR0801MB651 12, 51 -- X-OriginatorOrg: ucdavis.edu 12, 51 -- X-Greylist: Sender succeeded STARTTLS authentication, not delayed by milter-greylist-4.4.3 (smtp2.ucdavis.edu [128.120.32.8]); Tue, 31 Mar 2015 19:02:00 -0700 (PDT) 12, 51 -- X-Virus-Scanned: clamav-milter 0.98.1 at smtp2 12, 51 -- X-Virus-Status: Clean 12, 51 -- X-Scanned-By: MIMEDefang 2.74 12, 51 -- Content-Transfer-Encoding: 8bit 12, 51 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t312211I009135 ==============================End of - Headers==============================
At the risk of sounding too salsesman-y, I'd like to offer my thoughts on your situation.
First, congrats on having 3 FEGs at your facility! That's always a good problem to have! A couple things to consider:
As for the coaters, I would start with the following approach. First, figure out the size of the features you are looking for. You should have an idea of the grain size put down by each of your coaters. If the coating thickness or grain size exceeds the size of the features you are looking for, you will never see them. I would try stating it in such a way that you may be missing important information or obtaining inaccurate information from your samples because it is highly possible small features have been completely obscured by the thickness and or grain size of the metal coating you are currently using. I would recommend either an osmium coater or a high vacuum iridium or platinum coater, as you will be able to lay down much thinner coatings with grain sizes that may not even be visible.
If you are working at very low accelerating voltages, know that almost all samples, regardless of how carefully they were prepared or stored, build up a thin layer of hydrocarbon material on the surface. When working below 2kV, this contamination contributes significantly to the image formed. Ideally it should be removed with a UV cleaning cycle (although a very low power plasma clean may work on some materials). Doing so may will give a much better, and more accurate surface image, and may eliminate the need for a metal coating in some instances.
In the end, I think a valid way to frame your request is by stating that you want to use the equipment and tools that will get you the most accurate data from your samples, and not leave your results open to questioning or second guessing. If it results in saving time (samples come out right the first time) or money (new systems come with warranties, less prone to breakdowns) that might also help.
Best of luck! If you would like to continue this conversation offline, I would be happy to.
Cheers,
Jeff
jhall-at-2spi.com
Jeffrey A. Hall | Microscopist SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA 1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook
Dismclaimer: SPI Supplies manufactures and distributes tools for people working in microscopy laboratories
________________________________________ X-from: fahayes-at-ucdavis.edu {fahayes-at-ucdavis.edu} Sent: Tuesday, March 31, 2015 10:19 PM To: Jeff Hall
Listers,
We have 3 FEI FEG SEMs in our building. A new FEI FIB, a 4 yr old FEI nanoSEM and a 15 yr old FEI XL30 SFEG
We have 2 older sputter coaters. A Polaron E5100 (1988) and a Denton Desk II. Both use mech pumps. Our Polaron is no longer working
The problem of course is the visible grain at high mags (} 50Kx) with gold, gold/palladium, etc. It doesn't matter if it's the Polaron or the Denton. Same result. Visible grain over 50Kx.
I need to justify with written rationale to our directors why we should consider buying a high res coater. Simply stating "to compliment our 3 FEG SEMs" is not enough of a reason to spend the money. They prefer I fix the Polaron.
Any ideas/feedback from those who have already crossed this bridge would be helpful.
Thank you in advance
Also, does anyone have a resource for parts for Polaron coaters circa 1988 models?
Fred Hayes Manager, AMCaT Labs CHMS, College of Eng UC Davis
==============================Original Headers============================== 12, 51 -- From fahayes-at-ucdavis.edu Tue Mar 31 21:02:02 2015 12, 51 -- Received: from smtp2.ucdavis.edu (smtp2.ucdavis.edu [128.120.32.219]) 12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t312211I009135 12, 51 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2015 21:02:02 -0500 12, 51 -- Received: from exhub.ex.ad3.ucdavis.edu (mailbag.housing.ucdavis.edu [169.237.229.112]) 12, 51 -- by smtp2.ucdavis.edu (8.14.4/8.14.5/it-oel6-mimedefang-smtp-1.9) with ESMTP id t3121mde006125 12, 51 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=OK) 12, 51 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2015 19:02:00 -0700 12, 51 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (207.46.163.184) 12, 51 -- by exhub.ex.ad3.ucdavis.edu (169.237.229.112) with Microsoft SMTP Server 12, 51 -- (TLS) id 14.3.224.2; Tue, 31 Mar 2015 19:01:58 -0700 12, 51 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com (10.242.127.155) by 12, 51 -- DM2PR0801MB651.namprd08.prod.outlook.com (10.242.127.154) with Microsoft SMTP 12, 51 -- Server (TLS) id 15.1.118.21; Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) by 12, 51 -- DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) with mapi id 12, 51 -- 15.01.0118.022; Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 12, 51 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 51 -- Subject: rationale for buying a high res sputter coater 12, 51 -- Thread-Topic: rationale for buying a high res sputter coater 12, 51 -- Thread-Index: AdBsHbeDSWYu9NZsSriicsUEbzfugg== 12, 51 -- Date: Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- Message-ID: {DM2PR0801MB652387327D70F18ECE1AC91BEF30-at-DM2PR0801MB652.namprd08.prod.outlook.com} 12, 51 -- Accept-Language: en-US 12, 51 -- Content-Language: en-US 12, 51 -- X-MS-Has-Attach: 12, 51 -- X-MS-TNEF-Correlator: 12, 51 -- x-originating-ip: [169.237.64.26] 12, 51 -- authentication-results: microscopy.com; dkim=none (message not signed) 12, 51 -- header.d=none; 12, 51 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB651; 12, 51 -- x-forefront-antispam-report: BMV:1;SFV:NSPM;SFS:(10009020)(6009001)(504964003)(102836002)(50986999)(54356999)(40100003)(86362001)(87936001)(450100001)(76576001)(2501003)(2656002)(122556002)(77156002)(92566002)(2900100001)(2351001)(229853001)(62966003)(77096005)(90282001)(33656002)(74316001)(107886001)(46102003)(110136001)(99286002)(88552001)(75432002)(89122001);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR0801MB651;H:DM2PR0801MB652.namprd08.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 12, 51 -- x-microsoft-antispam-prvs: {DM2PR0801MB651C26D2D1ADE9C9B84F910BEF30-at-DM2PR0801MB651.namprd08.prod.outlook.com} 12, 51 -- x-exchange-antispam-report-test: UriScan:; 12, 51 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5002010)(5005006);SRVR:DM2PR0801MB651;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB651; 12, 51 -- x-forefront-prvs: 053315510E 12, 51 -- Content-Type: text/plain; charset="us-ascii" 12, 51 -- MIME-Version: 1.0 12, 51 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 01 Apr 2015 02:01:56.1550 12, 51 -- (UTC) 12, 51 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 12, 51 -- X-MS-Exchange-CrossTenant-id: a8046f64-66c0-4f00-9046-c8daf92ff62b 12, 51 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR0801MB651 12, 51 -- X-OriginatorOrg: ucdavis.edu 12, 51 -- X-Greylist: Sender succeeded STARTTLS authentication, not delayed by milter-greylist-4.4.3 (smtp2.ucdavis.edu [128.120.32.8]); Tue, 31 Mar 2015 19:02:00 -0700 (PDT) 12, 51 -- X-Virus-Scanned: clamav-milter 0.98.1 at smtp2 12, 51 -- X-Virus-Status: Clean 12, 51 -- X-Scanned-By: MIMEDefang 2.74 12, 51 -- Content-Transfer-Encoding: 8bit 12, 51 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t312211I009135 ==============================End of - Headers==============================
==============================Original Headers============================== 29, 28 -- From jhall-at-2spi.com Wed Apr 1 07:36:28 2015 29, 28 -- Received: from SPI-EX-01.spi.local (mail.2spi.com [209.120.197.107]) 29, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t31CaSv0015083 29, 28 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 07:36:28 -0500 29, 28 -- Received: from SPI-EX-01.spi.local (172.25.75.31) by SPI-EX-01.spi.local 29, 28 -- (172.25.75.31) with Microsoft SMTP Server (TLS) id 15.0.847.32; Wed, 1 Apr 29, 28 -- 2015 08:35:35 -0400 29, 28 -- Received: from SPI-EX-01.spi.local ([fe80::f8c4:9b23:d8de:fa70]) by 29, 28 -- SPI-EX-01.spi.local ([fe80::f8c4:9b23:d8de:fa70%12]) with mapi id 29, 28 -- 15.00.0847.030; Wed, 1 Apr 2015 08:35:35 -0400 29, 28 -- From: Jeff Hall {jhall-at-2spi.com} 29, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 29, 28 -- Subject: RE: [Microscopy] rationale for buying a high res sputter coater 29, 28 -- Thread-Topic: [Microscopy] rationale for buying a high res sputter coater 29, 28 -- Thread-Index: AQHQbCI+vtFjTwF650qdZXTql9qpgZ04ESZK 29, 28 -- Date: Wed, 1 Apr 2015 12:35:35 +0000 29, 28 -- Message-ID: {dc3081fa7e3c40dfa7d25d179b1d350c-at-SPI-EX-01.spi.local} 29, 28 -- References: {201504010219.t312JaHa022597-at-ns.microscopy.com} 29, 28 -- In-Reply-To: {201504010219.t312JaHa022597-at-ns.microscopy.com} 29, 28 -- Accept-Language: en-US 29, 28 -- Content-Language: en-US 29, 28 -- X-MS-Has-Attach: 29, 28 -- X-MS-TNEF-Correlator: 29, 28 -- x-originating-ip: [172.25.75.111] 29, 28 -- Content-Type: text/plain; charset="us-ascii" 29, 28 -- MIME-Version: 1.0 29, 28 -- Content-Transfer-Encoding: 8bit 29, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t31CaSv0015083 ==============================End of - Headers==============================
The University of Bath is holding a one-day Microscopy and Analysis Conference on 6th May 2015.
We have some excellent speakers for the Conference - see http://blogs.bath.ac.uk/mas/ for details of speakers, information about the trade exhibition and a conference program to download.
There will be a free buffet lunch and wine/beer reception for delegates!
It is free to sign up for the conference - just email Ursula Potter at University of Bath (u.j.potter-at-bath.ac.uk).
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Email: rhsia-at-umaryland.edu Name: Ru-ching Hsia
Organization: University of Maryland, Baltimore
Title-Subject: [Filtered] 2015 UMB Current Electron Microscopy Techniques Workshop
Message: Dear Colleagues,
We are pleased to announce that the Electron Microscopy Core Imaging facility (EMCIF) at the University of Maryland Baltimore will be hosting the Second Annual Current EM Techniques Workshop on May 28th and 29th, 2015.
The focus of this yearÂs workshop is immuno electron microscopy. The workshop will include oral presentations and tips-and-tricks discussion forums during the morning session followed by live instrument demonstrations and hands-on practice in the afternoon session.
Dr. Kent McDonald will be keynote speaker this year. There will be four tips and trick discussion forum sessions led by experienced panelists to discuss various aspects of immuno EM methods and techniques. Demo instruments will feature high pressure freezer, freeze substitution systems, a cryo-ultramicrotome, a grid plunger, cryo-TEM, cryo-SEM and possibly a CLEM system.
A dinner event will be co-sponsored by the Chesapeake Microscopy and Microanalysis society (CMMS) on May 28th providing opportunities for social and scientific exchange among workshop participants and CMMS members. More information regarding the workshop and registration can be found on our website
Please email coreimaging-at-umaryland.edu for any enquiries about the workshop.
We look forward to welcoming you in Baltimore in May.
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Email: dan.fairweather-at-delphi.com Name: Dan Fairweather
Organization: Delphi Automotive, Powertrain Div
Title-Subject: [Filtered] Diagnosing problems with a carbon coater
Message: Our lab had an EMS 450 carbon coater sitting on the counter top. I located the roughing pump in storage and am trying to make the system operational. All electrical operation seems to be working. I dumped out the old pump oil and replaced with new oil. Upon first pumping down the system, the vacuum gauge leveled at 5x10-1 mbar. I worked with the vacuum pump connections and was able to obtain 2x10-1 mbar. I ordered new seals for the jar that forms the vacuum chamber [the old seals are at least 10yrs old]. The new seals just came in, and there was no improvement to the level of vacuum. One of the observations that I have made is that I obtain the best vacuum when I turn on the system in the morning. If I leave the system running, the vacuum level will steadily worsen, holding finally at 5x10-1 mbar. Once I have cycled the system, I can never reach that level again unless I wait until the next day.
Any suggestions from the community? Do I have a pump problem or a vacuum gauge problem?
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Email: aheiss-at-amnh.org Name: Aaron Heiss
Organization: American Museum of Natural History
Title-Subject: [Filtered] TEM - MT2 Ultramicrotome Operation
Message: Hello all! I am a moderately experienced ultramicrotomist who learned on a Leica UC7, and subsequently used a Reichert-Jung Ultracut E. I'm now working with a Sorvall MT2, and while the unit is well-maintained and I can get good sections from it, I'm not sure exactly how thick it's cutting.* I know that the "main" setting is done with the knob on the top (which is marked for thickness in tens-of-Angstroms, better known as nanometres) but that this is affected by the wheel on the left of the front panel, and I'm not really sure how, or what to make of the markings (0 to 18). So, my question is this: how exactly does one set a precise thickness on the MT2?
* Yes, I can use interference colours, or check the thickness of folds, but this only measures the thickness of the sections, not how much material was cut from the block. In other words, it doesn't account for compression, which is of course variable both before and after spreading (I use chloroform vapours for this). The amount cut from the block is important because I'm doing 3-D reconstruction of serial sections, which means that I need to know exactly how far apart the sections are in the block.
TIA! Aaron
Aaron A. Heiss, Ph.D. Gerstner Scholar and Lerner-Gray Fellow Eunsoo Kim Laboratory Department of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York, NY 10024 USA
212-769-5838 aheiss-at-amnh.org
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Email: joseph.n.madary.civ-at-mail.mil Name: nick madary
Organization: joint pathology center(US Gov)
Title-Subject: [Filtered] Need to purchase a TEM with EDS any suggestions?
Message: I am sorry if this as a duplicate. In the market for a TEM with EDS and top notch imaging. If anyone has any suggestions on what not to buy and there are vendors out there please help. We do strictly biologicals(but do have a need for elemental analysis some times). regards, Nick
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==============================Original Headers============================== 11, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Apr 1 18:01:40 2015 11, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t31N1ePZ007852 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 18:01:40 -0500 11, 17 -- Message-ID: {551C78D4.6070207-at-microscopy.com} 11, 17 -- Date: Wed, 01 Apr 2015 18:01:40 -0500 11, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 11, 17 -- MIME-Version: 1.0 11, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 17 -- Subject: viaWWW:Need to purchase a TEM with EDS any suggestions? 11, 17 -- References: {201504011940.t31JesEE001574-at-ns.microscopy.com} 11, 17 -- In-Reply-To: {201504011940.t31JesEE001574-at-ns.microscopy.com} 11, 17 -- X-Forwarded-Message-Id: {201504011940.t31JesEE001574-at-ns.microscopy.com} 11, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I'm liking my Hitachi HT7700 all digital (no film) TEM. It strikes me as being very suitable for pathology. Hitachi would probably be glad to put you in contact with a couple of path labs I know of who bought one. EDS is available for that TEM, but I do not have it.
Aloha, Tina
} } Email: joseph.n.madary.civ-at-mail.mil } Name: nick madary } } Organization: joint pathology center(US Gov) } } Title-Subject: [Filtered] Need to purchase a TEM with EDS any suggestions? } } Message: I am sorry if this as a duplicate. In the market for a TEM with } EDS and top notch imaging. } If anyone has any suggestions on what not to buy and there are vendors out } there please help. We do } strictly biologicals(but do have a need for elemental analysis some times). } regards, Nick } } Login Host: 143.85.107.18 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original } Headers============================== } 11, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Apr 1 } 18:01:40 2015 } 11, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com } [206.69.208.22]) } 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } t31N1ePZ007852 } 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 18:01:40 } -0500 } 11, 17 -- Message-ID: {551C78D4.6070207-at-microscopy.com} } 11, 17 -- Date: Wed, 01 Apr 2015 18:01:40 -0500 } 11, 17 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 11, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 11, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) } Gecko/20100101 Thunderbird/31.5.0 } 11, 17 -- MIME-Version: 1.0 } 11, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 17 -- Subject: viaWWW:Need to purchase a TEM with EDS any suggestions? } 11, 17 -- References: {201504011940.t31JesEE001574-at-ns.microscopy.com} } 11, 17 -- In-Reply-To: {201504011940.t31JesEE001574-at-ns.microscopy.com} } 11, 17 -- X-Forwarded-Message-Id: } {201504011940.t31JesEE001574-at-ns.microscopy.com} } 11, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 17 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 21 -- From tina-at-pbrc.hawaii.edu Wed Apr 1 19:27:30 2015 7, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t320RUPd021941 7, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 19:27:30 -0500 7, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id t320RTFI012672 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 7, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 14:27:29 -1000 (HST) 7, 21 -- Received: from localhost (tina-at-localhost) 7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id t320RTGg012668 7, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 14:27:29 -1000 (HST) 7, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 7, 21 -- Date: Wed, 1 Apr 2015 14:27:29 -1000 (HST) 7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 21 -- X-X-Sender: tina-at-b1000 7, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 21 -- Subject: Re: [Microscopy] viaWWW:Need to purchase a TEM with EDS any suggestions? 7, 21 -- (fwd) 7, 21 -- Message-ID: {Pine.GSO.4.64.1504011426550.7593-at-b1000} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Plenty of variables to consider, but perhaps most straight-forward is that if the seals in your roughing pump are as old as the jar seals AND it was sitting around with used oil in it, you probably should consider a pump rebuild since there's no telling how bad things might be inside the pump. You might also check out the literature on your pump if any is available to see what vacuum levels it's capable of when working perfectly so that you can determine a real target vacuum to aim for (not knowing the details of the pump, for all we know you're actually within spec, poor as it might be).
-John
On April 1, 2015 7:26:26 PM EDT,microscopylistserver-noreply-at-microscopy.com wrote: --| --| --| --|---------------------------------------------------------------------------- --|The Microscopy ListServer -- CoSponsor: The Microscopy Society of --|America
Do you have the manual? If not, I can send a pdf of it. Basically, the knob on top is thickness in Ĺngstroms, and the thickness wheel on the left is a multiplier of the knob value. So: knob at 100 X wheel = thickness in Ĺngstroms How accurate this is depends on how well maintained and how worn is your MT-2. Particularly the Nylon block that rests on the advance screw.
Phil
} Email: aheiss-at-amnh.org } Name: Aaron Heiss } } Organization: American Museum of Natural History } } Title-Subject: [Filtered] TEM - MT2 Ultramicrotome Operation } } Message: Hello all! I am a moderately experienced ultramicrotomist who learned on a Leica UC7, and } subsequently used a Reichert-Jung Ultracut E. I'm now working with a Sorvall MT2, and while the } unit is well-maintained and I can get good sections from it, I'm not sure exactly how thick it's } cutting.* I know that the "main" setting is done with the knob on the top (which is marked for } thickness in tens-of-Angstroms, better known as nanometres) but that this is affected by the wheel } on the left of the front panel, and I'm not really sure how, or what to make of the markings (0 to } 18). So, my question is this: how exactly does one set a precise thickness on the MT2? } } * Yes, I can use interference colours, or check the thickness of folds, but this only measures the } thickness of the sections, not how much material was cut from the block. In other words, it doesn't } account for compression, which is of course variable both before and after spreading (I use } chloroform vapours for this). The amount cut from the block is important because I'm doing 3-D } reconstruction of serial sections, which means that I need to know exactly how far apart the } sections are in the block. } } TIA! } Aaron } } } Aaron A. Heiss, Ph.D. } Gerstner Scholar and Lerner-Gray Fellow } Eunsoo Kim Laboratory } Department of Invertebrate Zoology } American Museum of Natural History } Central Park West at 79th Street } New York, NY 10024 USA } } 212-769-5838 } aheiss-at-amnh.org -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 32 -- From oshel1pe-at-cmich.edu Thu Apr 2 08:56:50 2015 4, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t32DungD025412 4, 32 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2015 08:56:50 -0500 4, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 4, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t32DuZUc008144; 4, 32 -- Thu, 2 Apr 2015 09:56:48 -0400 4, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 4, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 4, 32 -- (TLS) id 14.3.195.1; Thu, 2 Apr 2015 09:56:40 -0400 4, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 4, 32 -- 14.3.195.1; Thu, 2 Apr 2015 09:56:39 -0400 4, 32 -- Message-ID: {551D4A97.5070609-at-cmich.edu} 4, 32 -- Date: Thu, 2 Apr 2015 09:56:39 -0400 4, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 32 -- MIME-Version: 1.0 4, 32 -- To: {aheiss-at-amnh.org} , micro {microscopy-at-microscopy.com} 4, 32 -- Subject: Re: [Microscopy] viaWWW:TEM - MT2 Ultramicrotome Operation 4, 32 -- References: {201504012330.t31NUYjN008255-at-ns.microscopy.com} 4, 32 -- In-Reply-To: {201504012330.t31NUYjN008255-at-ns.microscopy.com} 4, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 32 -- Content-Transfer-Encoding: 8bit 4, 32 -- X-Originating-IP: [141.209.2.100] 4, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 32 -- X-Spam-Score: -1.50 () [Hold at 6.00] RDNS_NONE,_L_IAA,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 4, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 4, 32 -- X-CanItPRO-Stream: default 4, 32 -- X-Canit-Stats-ID: 02Ob1UMkC - 05044bef603b - 20150402 4, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Dan's right - There are a lot of variables to consider. I'll add a few more :)
The fact that your vacuum worsens as time elapses makes me wonder if it is backstreaming oil into your carbon coater. A quick check of the vacuum line should let you know. If it is, that's the first thing to take care of before you contaminate the whole system with oil.
Assuming there is no backstreaming occurring, if you have access to a vacuum meter, you might want to attach it directly to the pump and see what kind of vacuum the pump is pulling on its own (no coater, no vacuum tubing). That should tell you which side the problem is on. If you don't have a vacuum meter, try to find a second pump to try out on the system to confirm the vacuum you can pull.
If it's the pump, a rebuild or a new pump is probably the best option. My personal experience with rebuilds has been about 50/50, for what it's worth.
If the pump seems fine and the problem seems to be on the coater side, I would start by removing the bell jar and plugging the vacuum inlet in the chamber with a stopper to see again which side the problem is on - the chamber itself, or the internals. From there, it becomes a matter of trying to check seals to find the leak.
I hope that helps - best of luck!
Jeff
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Dan,
Plenty of variables to consider, but perhaps most straight-forward is that if the seals in your roughing pump are as old as the jar seals AND it was sitting around with used oil in it, you probably should consider a pump rebuild since there's no telling how bad things might be inside the pump. You might also check out the literature on your pump if any is available to see what vacuum levels it's capable of when working perfectly so that you can determine a real target vacuum to aim for (not knowing the details of the pump, for all we know you're actually within spec, poor as it might be).
-John
On April 1, 2015 7:26:26 PM EDT,microscopylistserver-noreply-at-microscopy.com wrote: --| --| --| --|--------------------------------------------------------------------- --|------- The Microscopy ListServer -- CoSponsor: The Microscopy --|Society of America To Subscribe/Unsubscribe -- --|http://www.microscopy.com/MicroscopyListserver --|On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html --|--------------------------------------------------------------------- --|------- --| --|X-from:dan.fairweather-at-delphi.com --| --|This Question/Comment was submitted to the Microscopy Listserver --|using the WWW based Form athttp://www.microscopy.com/MLFormMail.html --|--------------------------------------------------------------------- --|------ Remember this posting is most likely not from a Subscriber, so --|when replying --|please copy bothdan.fairweather-at-delphi.com as well as the --|Microscopy Listserver --|--------------------------------------------------------------------- --|------ --| --|Email:dan.fairweather-at-delphi.com --|Name: Dan Fairweather --| --|Organization: Delphi Automotive, Powertrain Div --| --|Title-Subject: [Filtered] Diagnosing problems with a carbon coater --| --|Message: Our lab had an EMS 450 carbon coater sitting on the counter --|top. I located the roughing pump in storage and am trying to make the --|system operational. All electrical operation seems to be working. I --|dumped out the old pump oil and replaced with new oil. Upon first --|pumping down the system, the vacuum gauge leveled at 5x10-1 mbar. I --|worked with the vacuum pump connections and was able to obtain 2x10-1 --|mbar. I ordered new seals for the jar that forms the vacuum chamber --|[the old seals are at least 10yrs old]. The new seals just came in, --|and there was no improvement to the level of vacuum. One of the --|observations that I have made is that I obtain the best vacuum when I --|turn on the system in the morning. If I leave the system running, the --|vacuum level will steadily worsen, holding finally at 5x10-1 mbar. --|Once I have cycled the system, I can never reach that level again --|unless I wait until the next day. --| --|Any suggestions from the community? Do I have a pump problem or a --|vacuum gauge problem? --| --| Login Host: 216.82.243.84 --| Listserver Email Form V - 20120416 --|--------------------------------------------------------------------- --|------ --| --| --|-- --|=========================================== --|Do not reply to this message it is from the Microscopy Listserver --|NO-REPLY forwarding system. You should send a new message to --| --|Microscopy-at-Microscopy.com --| --|============================================
Hi Phil -- I don't think we do have a manual -- and whether or not we do, a PDF would be most welcome!
I should've mentioned in my original post that I'd been told that the two numbers were multiplied, but I was beginning to suspect that I'd misunderstood. This is because I'd set the top knob to my desired thickness (50 nm in this case) and the front wheel to 1, and was getting no advance at all. Setting the front wheel to 2 gave different results depending on whether I'd dialed up or down to get there. Others have told me that the best practice is to set the top knob to something much lower and dial the front wheel up -- so for 50 nm I should set the top knob to 10 and the front wheel to 5, or vice versa. I'll give that a try and see if it works.
Many thanks, to you and to everyone else who responded via e-mail!! Aaron
Aaron A. Heiss, Ph.D. Gerstner Scholar and Lerner-Gray Fellow Eunsoo Kim Laboratory Department of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York, NY 10024 USA
212-769-5838 aheiss-at-amnh.org
________________________________________ X-from: Philip Oshel [oshel1pe-at-cmich.edu] Sent: April 2, 2015 9:56 AM To: Aaron Heiss; micro
X-from: joseph.uknalis-at-ars.usda.gov
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA
Title-Subject: [Filtered] Denton DCP-1 critical point dryer gasket
Message: I got replacement gaskets for the pressure chamber from Denton because the existing one was very old (cracking, leaking). Popped the new one on and threw the old in the trash. The new ones looked thicker than the old but I chalked it up to age...
Now when I bleed gas into the chamber the gasket pops out of place no matter how tight I screw down the clamp. Mercifully at a low pressure { 50 psi.
Our instrument is pretty old, prob from 1972. I got the sense that the new ones have a groove that the o-ring sits in...
Can anyone verify this? Anyone know of a source for a slightly thinner O-ring that will fit??
thanks
Joe
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Email: Pruessner-at-ukzn.ac.za Name: Karin Pruessner
Organization: University of KwaZulu-Natal
Title-Subject: [Filtered] Postdoctoral Research Position
Message: The University of KwaZulu-Natal in beautiful Durban, South Africa, is home to an interdisciplinary Nanotechnology Platform consisting of 4 pillars in Nano Energy, Nano Materials, Nano Health and Quantum. The Nano Energy pillar has a position available for a Postdoctoral Researcher to be filled as soon as possible. Our team consists of Chemists, Physicists, Materials Scientists, and Engineers. We are working on the development of an off-grid refrigeration unit for rural areas in Africa. We are looking for a Materials Scientist with a solid background in Materials Characterization to join us. Neighboring fields will be considered. The successful candidate should have experience in nanotechnology and at least one of the following areas:  Nano Photovoltaics  Nano Energy Storage  Nano Cooling Fluids  Graphene Hands-on experience in X-Ray Diffraction, Scanning and Transmission Electron Microscopy and Raman Spectroscopy is expected. Additional expertise in Materials Synthesis would be advantageous. Interested candidates should send their electronic application materials to: Dr Karin Pruessner, Coordinator Nano Energy School of Chemistry and Physics University of KwaZulu-Natal - Westville Campus University Road Durban, 4000 South Africa Ph: ++27 31 260-7660 e-mail: Pruessner-at-ukzn.ac.za
The post is initially for one year with the possibility of renewal. Review of applications will begin immediately.
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Email: gary-at-gaugler.com Name: Dr. Gary Gaugler
Organization: Here and there
Title-Subject: [Filtered] Bruker EDS system
Message: Does anyone know about this Bruker EDS system and what its used price might be? I assume that it is an SDD. I'm not familiar with Bruker. Details of the system are below.
Bruker EDS detector
X-Flash Detector 3001: 10 mm2, FWHM { 129 Quantax single processing unit SVE Quantax workstation class computer Quantax IO-scan system and interface Esprit integrated manual
Software for Bruker EDS: Esprit Spectrum based Esprit Quant Esprit E-quant Esprit HS-quant Esprit Scan Esprit Line Esprit Map Esprit Multi-point Esprit Project Esprit Report Esprit SEMlink Esprit U-quant
What is an SVE?
TIA, gary g.
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==============================Original Headers============================== 20, 17 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 4 17:08:20 2015 20, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t34M8K5Y003547 20, 17 -- for {microscopy-at-microscopy.com} ; Sat, 4 Apr 2015 17:08:20 -0500 20, 17 -- Message-ID: {552060D3.3050901-at-microscopy.com} 20, 17 -- Date: Sat, 04 Apr 2015 17:08:19 -0500 20, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 20, 17 -- MIME-Version: 1.0 20, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 17 -- Subject: viaWWW:Bruker EDS system info needed 20, 17 -- References: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- In-Reply-To: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- X-Forwarded-Message-Id: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, may i ask if any of you have the chance to try both Gatan Cryo/ LN2 Cold Stage Holder Model 636 and CHDT3504? Are both holder performing equally well? Any pros and cons?
Cheers, Yee Yan, Tay Nanyang Technological University
==============================Original Headers============================== 2, 28 -- From rongchigram79-at-yahoo.com.sg Sun Apr 5 09:35:48 2015 2, 28 -- Received: from nm24-vm3.bullet.mail.sg3.yahoo.com (nm24-vm3.bullet.mail.sg3.yahoo.com [106.10.151.82]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t35EZkXO005663 2, 28 -- for {Microscopy-at-microscopy.com} ; Sun, 5 Apr 2015 09:35:47 -0500 2, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1428241690; bh=v5del0jaRsrAATLocxaRLbJtBlX4YGHLd8Prx6xaFj8=; h=Date:From:Reply-To:To:Subject:From:Subject; b=Z+gO8CYg7aOkxzR+ITbTLNDuvpy2P12ikqL0eBq0+8PPocizuC5neSCZiI8scOprfNhc+d0jfmp74fMbYNub9juXdnsvEduvhC+nTUuMdKWgOZ4KOvvENWYBxSJAQ3RySa2sZTlthVcY0IFL9NqfABY3IRqDNEZzLRJgvPIdTBfkMiVnHI3xJMJs7ZAtHfHXPCuUPHkzGupFTQo+LBXlFILnmrmpe8SYJ8Z3dDtu1hEWophBQmQe6WZknPIy8oMxYsN6xybHQCOvP64/XRWufQhoruShv6ogmNYzOs5qZ/8mQaVJERWio4MfVtd69K7ExxFlsRZIFK57Hir51jumWg== 2, 28 -- Received: from [106.10.166.120] by nm24.bullet.mail.sg3.yahoo.com with NNFMP; 05 Apr 2015 13:48:10 -0000 2, 28 -- Received: from [106.10.151.250] by tm9.bullet.mail.sg3.yahoo.com with NNFMP; 05 Apr 2015 13:48:10 -0000 2, 28 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com with NNFMP; 05 Apr 2015 13:48:10 -0000 2, 28 -- X-Yahoo-Newman-Property: ymail-3 2, 28 -- X-Yahoo-Newman-Id: 1396.74919.bm-at-omp1021.mail.sg3.yahoo.com 2, 28 -- X-YMail-OSG: hDuiypAVM1ndP5yD74mEkK3Q0WpV3gWvvQmqoZ8cPZlQGCCM_sGHDj3zDwQzcB0 2, 28 -- nM3v.My8j9PGoybqOYDFooDOMFy.DbZarcuffh.DSSDFDV.6LsrhifrOlPfN6JrRwRJzywI5I40s 2, 28 -- MksSFgeTEf3ulelpafM0iIW9YuhtQhNrG5baVI5VmnV1AYiOYD.nGB1eqrZgkvf49PnDNPfH5OOL 2, 28 -- ZWFaBYsIIocpkFWQ.vkdQkpIY.G7EBvJULwiPW6WBTqBiDxQ.o2z58cN3aaxj.0AB8mTvCHue510 2, 28 -- ub5a1ifSIBcdlwCZCjPdTQ6TD2Q_o3CS1b6sq3ZfiYD9CoAS3J3Vf2xsCvOwCMMfMGueZxo0E.pp 2, 28 -- jbMxv4qy3cCr8qxAuFjVBvgkALdAMhaqnml8faxMGTjgPNmHqqzsg81j59u5qOJcMghcGi9fU_0e 2, 28 -- wXHOmXTPe9XIrL8S154MVyTViyBIzv5JXlA8ZWlHGOTfxue0JdjkJw1jw75gUahxzCQ-- 2, 28 -- Received: by 106.10.196.93; Sun, 05 Apr 2015 13:48:09 +0000 2, 28 -- Date: Sun, 5 Apr 2015 13:48:09 +0000 (UTC) 2, 28 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 2, 28 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 2, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 28 -- Message-ID: {2018643352.32987.1428241689237.JavaMail.yahoo-at-mail.yahoo.com} 2, 28 -- Subject: Anyone have tried both Gatan Cryo/ LN2 Cold Stage Holder Model 636 2, 28 -- and CHDT3504 2, 28 -- MIME-Version: 1.0 2, 28 -- Content-Type: text/plain; charset=UTF-8 2, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Gary, My lab has two Bruker EDS systems and they are great. In my opinion they are the best EDS systems on the market. Yes it has a silicon drift detector. I don't know what SVE stands for. 10mm2 window is small but will likely give you more counts than you are used to (50K to 100K cps). The software options are all the most basic ones.
The big thing that is missing is the HyperMap option, which in my opinion is one of the best things about the system. It allows you to go back to a map you collected a year ago and change the element list or create an EDS spectrum of a point or area on that map just like the sample were still in the SEM. But I think Bruker will let you add that or any other software option that you may want which is not already activated.
My guess is that the system is worth at least $15K to $20K depending on its age and condition. The guy you want to talk to about this system is Robert Brandon of Bruker (Robert.Brandom-at-bruker-axs.com). He is one of their sales managers and has been very helpful to me in the past. Ted Juzwak, Applications Lab Manager at Bruker (Ted.Juzwak-at-bruker-axs.com) is also a wealth of information on these systems.
You don't see X-Flash systems on the used equipment market very often though. They last a long time and are so good no one sells them to upgrade to a better system because there isn't anything better.
Good luck,
John Knowles President MicroVision Laboratories
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Saturday, April 04, 2015 6:31 PM To: John-at-microvisionlabs.com
X-from: gary-at-gaugler.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-gaugler.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-gaugler.com Name: Dr. Gary Gaugler
Organization: Here and there
Title-Subject: [Filtered] Bruker EDS system
Message: Does anyone know about this Bruker EDS system and what its used price might be? I assume that it is an SDD. I'm not familiar with Bruker. Details of the system are below.
Bruker EDS detector
X-Flash Detector 3001: 10 mm2, FWHM { 129 Quantax single processing unit SVE Quantax workstation class computer Quantax IO-scan system and interface Esprit integrated manual
Software for Bruker EDS: Esprit Spectrum based Esprit Quant Esprit E-quant Esprit HS-quant Esprit Scan Esprit Line Esprit Map Esprit Multi-point Esprit Project Esprit Report Esprit SEMlink Esprit U-quant
What is an SVE?
TIA, gary g.
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==============================Original Headers============================== 20, 17 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 4 17:08:20 2015 20, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t34M8K5Y003547 20, 17 -- for {microscopy-at-microscopy.com} ; Sat, 4 Apr 2015 17:08:20 -0500 20, 17 -- Message-ID: {552060D3.3050901-at-microscopy.com} 20, 17 -- Date: Sat, 04 Apr 2015 17:08:19 -0500 20, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 20, 17 -- MIME-Version: 1.0 20, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 17 -- Subject: viaWWW:Bruker EDS system info needed 20, 17 -- References: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- In-Reply-To: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- X-Forwarded-Message-Id: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 33, 28 -- From John-at-MicroVisionLabs.com Mon Apr 6 10:00:44 2015 33, 28 -- Received: from n55.mail01.mtsvc.net (mailout90.mail01.mtsvc.net [216.70.64.171]) 33, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t36F0iIe019801 33, 28 -- for {Microscopy-at-Microscopy.com} ; Mon, 6 Apr 2015 10:00:44 -0500 33, 28 -- Received: from c-24-61-207-227.hsd1.ma.comcast.net ([24.61.207.227]:37713 helo=JohnPC) 33, 28 -- by n55.mail01.mtsvc.net with esmtpa (Exim 4.72) 33, 28 -- (envelope-from {John-at-MicroVisionLabs.com} ) 33, 28 -- id 1Yf8W3-0002uC-Jh 33, 28 -- for Microscopy-at-Microscopy.com; Mon, 06 Apr 2015 11:00:44 -0400 33, 28 -- Reply-To: {John-at-MicroVisionLabs.com} 33, 28 -- From: "John Knowles" {John-at-MicroVisionLabs.com} 33, 28 -- To: {Microscopy-at-Microscopy.com} 33, 28 -- References: {201504042231.t34MVPWs010352-at-ns.microscopy.com} 33, 28 -- In-Reply-To: 33, 28 -- Subject: FW: [Microscopy] viaWWW:Bruker EDS system info needed 33, 28 -- Date: Mon, 6 Apr 2015 11:00:43 -0400 33, 28 -- Organization: MicroVision Labs, Inc. 33, 28 -- Message-ID: {016901d0707a$74774940$5d65dbc0$-at-MicroVisionLabs.com} 33, 28 -- MIME-Version: 1.0 33, 28 -- Content-Type: text/plain; 33, 28 -- charset="iso-8859-1" 33, 28 -- X-Mailer: Microsoft Outlook 14.0 33, 28 -- Thread-Index: AQI1z5VinkqMwXrQBMg+oxLBk+qp0Zx1SBZQgAANc+A= 33, 28 -- Content-Language: en-us 33, 28 -- X-Authenticated-User: 1235518 John-at-MicroVisionLabs.com 33, 28 -- X-MT-ID: 5872FFFCEE07D69BCD65B86159E3C59E569EAD28 33, 28 -- Content-Transfer-Encoding: 8bit 33, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t36F0iIe019801 ==============================End of - Headers==============================
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Email: scottserata-at-gmail.com Name: Scott Serata
Organization: California Academy of Sciences
Title-Subject: [Filtered] which SEM should we buy for biological/zoological work?
Message: Hi, I am the SEM engineer at the California Academy of Sciences in San Francisco. We have had a Zeiss/LEO 1450VP SEM for about 14 years. We are a zoological research organization with specialists in Entomology, Arachnology, Botany, Invertebrate Zoology, Herpetology, etc. I am starting the process of collecting information in order to purchase a new SEM. Any advice out there from people who do similar work?
Also if anyone has any feedback about the quality of field service from Zeiss, Hitachi, JEOL, FEI, etc.
Our SEM is generally used for imaging specimens using the conventional high-vacuum SE detector. Usually the specimens are coated using a gold/palladium sputter coater. We do need the ability to image uncoated specimens using either VPSE (variable pressure) or BSD. Our biggest problem with the old LEO 1450VP was the inability to get good sharp images on uncoated specimens due to charging. We generally do not need to look at wet specimens so we do not need very high chamber pressures. Currently I am looking at the Zeiss EVO MA 10 and the Hitachi SU 3500. We do not need any analytical detectors (no Xray EDS, etc) Max magnifications are perhaps 100kX. Tungsten filament electron gun is fine. We probably don't want to spend more than $200k. This is a big deal for us because we will probably be stuck with this machine for another 14 years so we want to make the right decision!
Thank you for your time! Scott Serata
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==============================Original Headers============================== 17, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 7 19:33:33 2015 17, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t380XX96025279 17, 17 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2015 19:33:33 -0500 17, 17 -- Message-ID: {5524775D.9030700-at-microscopy.com} 17, 17 -- Date: Tue, 07 Apr 2015 19:33:33 -0500 17, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 17, 17 -- MIME-Version: 1.0 17, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 17 -- Subject: viaWWW:which SEM should we buy for biological/zoological work? 17, 17 -- References: {201504072044.t37Ki9Hq009904-at-ns.microscopy.com} 17, 17 -- In-Reply-To: {201504072044.t37Ki9Hq009904-at-ns.microscopy.com} 17, 17 -- X-Forwarded-Message-Id: {201504072044.t37Ki9Hq009904-at-ns.microscopy.com} 17, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: joseph.n.madary.civ-at-mail.mil Name: Nick Madary
Organization: joint pathology center
Title-Subject: [Filtered] Reply about SEM purchase
Message: Hi Scott, You have come to the right place because I have had so much great info regarding a TEM c EDS purchase. I will tell you actually using Hitachis, they are really good, I lean to the 3500 just because of my apps. JEOL has a nice table top model that is excellent as wel, you almost do not even realize there is an SEM there, the computer screen is as large as the unit it seems. You are in an enviable position. I see you have money, but if not, there are some gov surplus sites that might have really decent scopes that were turned in due to BRAC. We will do one soon ourselves. Zeiss has always been awesome at TEM,so if you can choose go with Hitachit or even that nice, new small JEOL.
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Electron microprobe Cameca model Camebax MB1 with Tracor Northern ver. TN2000 electronic control console. Max. accelerating voltage of 45 kV Fitted with BSE detector, light microscope and three WDX spectrometers: Spectrometer 1 fitted with LIF, PET, TAP, and OdPb crystals Spectrometer 2 - PET and TAP Spectrometer 3 -LIF and PET Turbomolecular vacuum pump. Documentation and some spare parts are available.
Krassimir Bozhilov
bozhilov-at-ucr.edu
==============================Original Headers============================== 6, 34 -- From krassimir.bozhilov-at-ucr.edu Thu Apr 9 15:17:13 2015 6, 34 -- Received: from mx2.ucr.edu (mx2.ucr.edu [138.23.62.3]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t39KHCRS027756 6, 34 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2015 15:17:13 -0500 6, 34 -- X-IronPort-Anti-Spam-Filtered: true 6, 34 -- X-IronPort-Anti-Spam-Result: A2CFAAD23SZVnED4F4pcg1pcBcYih0pMAQEBAQEBEgEBAQEBCAsJCRQuhCY6UQE+QicEE4gqBQioIqE+AYUBAR+OEYFXg12BFgWvboQRb4FEfwEBAQ 6, 34 -- X-IPAS-Result: A2CFAAD23SZVnED4F4pcg1pcBcYih0pMAQEBAQEBEgEBAQEBCAsJCRQuhCY6UQE+QicEE4gqBQioIqE+AYUBAR+OEYFXg12BFgWvboQRb4FEfwEBAQ 6, 34 -- X-IronPort-AV: E=Sophos;i="5.11,552,1422950400"; 6, 34 -- d="scan'208";a="365523087" 6, 34 -- Received: from exch-edge-2.ucr.edu ([138.23.248.64]) 6, 34 -- by smtp2.ucr.edu with ESMTP/TLS/AES128-SHA; 09 Apr 2015 13:17:06 -0700 6, 34 -- Received: from EXCH-CLNT-4.exch.ucr.edu (138.23.249.31) by exch-edge-2.ucr.edu 6, 34 -- (138.23.248.64) with Microsoft SMTP Server (TLS) id 14.3.224.2; Thu, 9 Apr 6, 34 -- 2015 13:16:42 -0700 6, 34 -- Received: from EXCH-MBOX-6.exch.ucr.edu ([169.254.6.194]) by 6, 34 -- exch-clnt-4.exch.ucr.edu ([138.23.249.31]) with mapi id 14.03.0224.002; Thu, 6, 34 -- 9 Apr 2015 13:17:05 -0700 6, 34 -- From: Krassimir Bozhilov {krassimir.bozhilov-at-ucr.edu} 6, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 34 -- Subject: Cameca Camebax MB1 available 6, 34 -- Thread-Topic: Cameca Camebax MB1 available 6, 34 -- Thread-Index: AQHQcwIlMMuFu1pEOEWRxSUPRuKk0g== 6, 34 -- Date: Thu, 9 Apr 2015 20:17:04 +0000 6, 34 -- Message-ID: {162245AD-7BB7-4228-A150-A297AC2D0244-at-ucr.edu} 6, 34 -- Accept-Language: en-US 6, 34 -- Content-Language: en-US 6, 34 -- X-MS-Has-Attach: 6, 34 -- X-MS-TNEF-Correlator: 6, 34 -- x-originating-ip: [138.23.63.83] 6, 34 -- Content-Type: text/plain; charset="us-ascii" 6, 34 -- Content-ID: {685C9C7010304647949395D07A28C43E-at-exch.ucr.edu} 6, 34 -- MIME-Version: 1.0 6, 34 -- Content-Transfer-Encoding: 8bit 6, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t39KHCRS027756 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Preparing gold samples for TEM
Message: Does anyone have experienced preparing gold samples for use in a TEM. I have FIB sections but there seems to be gallium from the milling present on the surface. Contact me if you would like to see the image. I understand that gold is very soft and keeping a natural texture is problematic. Any advise would be great.
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Immunogold labeling
Message: I want to label toxin A & B in C. Difficile bacterial with immunogold, so which antigen retrieval solution should I use or how to unmask the antigen before proceeding Immuno labeling on LR whte sections. Is there any universal or general method is available to unmask antigen or it should be antigen specific.?
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The Southeastern North Carolina Regional Microanalytical and Imaging Center (SENCR-MIC) at Fayetteville State University (FSU) is seeking applications for a Research Associate staff position, working primarily on a world-class Field-Emission Electron Probe Microanalyzer (EPMA) and a Scanning Electron Microscope (SEM), and other analytical instruments including AFM, XRD. The initial position is for two years, while extension is possible contingent on the performance and funding.
The successful candidate will be responsible for the day to day operations and maintenance of instruments at SENCR-MIC under the supervision of the SENCR-MIC Director. Major duties include training diverse internal and external users on the instruments, providing technical assistance to users, conducting professional services based on user fees, teaching student labs for formal courses, and other duties assigned by the Director.
Requirements: Minimum of Master's Degree (Ph.D. preferred) in Geosciences, Materials Science, Engineering, Chemistry, Physics or other related disciplines. Experiences in EPMA or SEM analysis are required, and experiences in AFM, XRD and TEM are optimum. The successful candidate must be highly dedicated to professional services, possess outstanding oral and written communication skills, and must be able to work with a wide range of users including faculty, staff, visiting scientists and students under supervision of the Director.
Application to this position should be made online at https://jobs.uncfsu.edu/postings/10917.
Fayetteville State University is committed to equality of educational opportunity and employment and does not discriminate against applicants, students, or employees based on race, color, national origin, religion, sex, gender identity, sexual orientation, age, disability, genetic information or veteran status. Moreover, Fayetteville State University values diversity and actively seeks to recruit talented students, faculty, and staff from diverse backgrounds.
==============================Original Headers============================== 9, 21 -- From zhiping_luo-at-hotmail.com Thu Apr 9 20:15:08 2015 9, 21 -- Received: from BAY004-OMC3S11.hotmail.com (bay004-omc3s11.hotmail.com [65.54.190.149]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3A1F8hf011157 9, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2015 20:15:08 -0500 9, 21 -- Received: from BAY175-W42 ([65.54.190.189]) by BAY004-OMC3S11.hotmail.com over TLS secured channel with Microsoft SMTPSVC(7.5.7601.22751); 9, 21 -- Thu, 9 Apr 2015 18:15:08 -0700 9, 21 -- X-TMN: [t+fFSseJThmdmZKkcyoI+VFmot7hgSqG] 9, 21 -- X-Originating-Email: [zhiping_luo-at-hotmail.com] 9, 21 -- Message-ID: {BAY175-W4206A1E7DD768A955C5EB08DFA0-at-phx.gbl} 9, 21 -- Reply-To: {zhiping_luo-at-hotmail.com} 9, 21 -- From: Zhiping LUO {zhiping_luo-at-hotmail.com} 9, 21 -- To: Microscopy {microscopy-at-microscopy.com} 9, 21 -- Subject: Research Associate Staff Position (mainly in EPMA) available at 9, 21 -- Fayetteville State University, North Carolina 9, 21 -- Date: Thu, 9 Apr 2015 20:15:08 -0500 9, 21 -- Importance: Normal 9, 21 -- Content-Type: text/plain; charset="iso-8859-1" 9, 21 -- MIME-Version: 1.0 9, 21 -- X-OriginalArrivalTime: 10 Apr 2015 01:15:08.0106 (UTC) FILETIME=[C8A922A0:01D0732B] 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3A1F8hf011157 ==============================End of - Headers==============================
McCrone Research Institute cordially invites you to participate in the 67th annual Inter/Micro conference by giving a presentation of your research paper. We are accepting presentation abstracts in techniques and instrumentation, environmental and industrial microscopy, and chemical and forensic microscopy. Presentations will be held at McCrone Research Institute on June 8-10.
The deadline to submit titles and abstracts is April 13, 2015. Click here to register.
Speakers will receive a $50 conference registration discount!
http://www.mcri.org/v/101/InterMicro
Tony
......................................... Andrew Anthony "Tony" Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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I am keeping alive (non-commercially) FIB-620 dual-beam FIB/SEM, which is based on the platform of Phillips XL-30/XL-40 SEM and used for student training and research projects that can't be done on brand-new instruments.
To keep it going I am looking for following parts:
If anyone will be disposing off old XL-30 or XL-40 SEM, or FIB-620 FIB I'd gladly accept it as "donation"
Thanks everyone beforehand,
-- Valery Ray - also with AIM/NISP Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
==============================Original Headers============================== 7, 30 -- From vray-at-partbeamsystech.com Fri Apr 10 10:08:22 2015 7, 30 -- Received: from nm23-vm1.bullet.mail.bf1.yahoo.com (nm23-vm1.bullet.mail.bf1.yahoo.com [98.139.213.141]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3AF8L3B006924 7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 10 Apr 2015 10:08:22 -0500 7, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1428678501; bh=Y/uGYBFJAcWJksdL8ZlBCdYVWFPQ9U5+CDm9FLF+aA0=; h=Date:From:To:Subject:From:Subject; b=MF4klSlMJbNLfQcNfCOFhDMwEdcMhhXK1SyI/NRknW9thm2Zhd49cPPsj+Z3F93LszvOj6QozBUKPRaYQNfnTkmIMZqcY9eM1EjPE6U9DBX9GuB8birN3W21T7SXbsgJdsVoMwVA+gczA3hiW7ae+wF1Qo9nz/bNLck6ffMdcFiB9C+nwKCifaC/P6+TzyXnw5YekfiuAtp3wp6NiMq+MR12t+m083J75KHHRaMOHUncttyj63ppKp/H1ykyz0N2Lsc+FoZxOGs8fhyz6LsXy825bIrBvhWG9/8LjxmG73IwX5HjrRIOISPYsHJD+vwl1WbEc52tRU8N5HvitzQVJg== 7, 30 -- Received: from [98.139.170.178] by nm23.bullet.mail.bf1.yahoo.com with NNFMP; 10 Apr 2015 15:08:21 -0000 7, 30 -- Received: from [68.142.230.75] by tm21.bullet.mail.bf1.yahoo.com with NNFMP; 10 Apr 2015 15:08:21 -0000 7, 30 -- Received: from [127.0.0.1] by smtp232.mail.bf1.yahoo.com with NNFMP; 10 Apr 2015 15:08:21 -0000 7, 30 -- X-Yahoo-Newman-Id: 590739.3789.bm-at-smtp232.mail.bf1.yahoo.com 7, 30 -- X-Yahoo-Newman-Property: ymail-3 7, 30 -- X-YMail-OSG: _iAg.fgVM1lAB0eiIBN85x8heelkWFLEO9yU1.SqD5c6Xic 7, 30 -- hXOK8EZTyhMXvavmDtczGJx9HtVfBelmGVt5TYmOTnTDlg5zjc21HXLZk_z7 7, 30 -- Soa_HjsgU76ziaypbiT8e898bB0lrKJ_TM9frQVe8yQg2iAyMGfK22bEAt8l 7, 30 -- ODAxe2bDcN63Sl2vPkFAZoyAd944tTce1fAAlc8PJFRNjuRvN51KAGGLqYvu 7, 30 -- 0Nt6ULCzm3u.6w7QSahBW_lStkf2HZkjFAKnRk5PslE515WLJ0pQreZeLoIQ 7, 30 -- SHKvmyBkSIwhw.V2iJedktRzvWgK0B8An6g9.n0eFWn.3HTjmBkOLJgQuDyb 7, 30 -- PSlf1yZE6DLNAvfb4p1mjmGIsDxbE0E8HvJOo6MKRpb0f3dYbNQxp5HTFZv9 7, 30 -- 8lixAxXrcB4sf8maAXk4StcLEWIuf0PbK34Bk.cgPDTXLLAGr5JoLAO78pR3 7, 30 -- WbbvvV_Np4m0ROuINWjw.OOTwRHeARQGtyyphbW1GZvenXvfd_cl_aU5jQKn 7, 30 -- X0dRXZUU2W4TyjOnk3Xr8exY4207QviwqVcWgrbXn 7, 30 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 30 -- Message-ID: {5527E763.8040705-at-partbeamsystech.com} 7, 30 -- Date: Fri, 10 Apr 2015 11:08:19 -0400 7, 30 -- From: Valery Ray {vray-at-partbeamsystech.com} 7, 30 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 7, 30 -- MIME-Version: 1.0 7, 30 -- To: microscopy-at-microscopy.com 7, 30 -- Subject: Looking for parts or parts or parts tool: XL-30, XL-40, or FIB-620 7, 30 -- Content-Type: text/plain; charset=utf-8; format=flowed 7, 30 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: Univ of Connecticut
Title-Subject: [Filtered] Access to User Facilities
Message: I am looking for advice from the community about how access to microscope facilities is granted to users. Specifically, I am thinking of something not quite so formal as the General User Access Proposal process available at National Labs, but not as open as our existing "free-for-all" where anyone who requests training can have open access.
I don't need advice on how to train users, rather, I need a fair process on how to determine who should or should not become a user.
Does anyone have a policy/process they wish to share--off line if you like--that weighs user requests? Perhaps a policy that includes various levels of access to users based on their needs and skills?
And a follow-up question is the magic "silver bullet" of how to monitor users after training to ensure that they are actually doing everything the way they were taught.
Thanks, Roger Ristau Univ of Connecticut
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Please send out a summary of replies once you get some. They can remain anonymous as far as I care.
We are probably a small enough lab that we have remained rather informal about access.
There are a few people that I have thought about redirecting away from the microscope as their brain just doesn't seem to be wired for that kind of work. I don't know if I have officially said anything out loud. Some seemed to have handed off SEM work to others in their research groups who are more adept.
Upon granting sign-up privileges, I advise new users whether they are free to sign up as they please or whether I would definitely like to be nearby for their next few sessions. Our reservation system, ORS, provides the option for ones to be a resource monitor by e-mail. I set that option for myself so I get notified about every change in the schedule. Most notices can be ignored. A few users are on my watch list. The delete button is easy to use for those that don't concern me.
We average about 25 hours of use per week. There is really no reason that ones HAVE to use the SEM outside of regular hours. A few users are granted or loaned keys for access at any time. Sometimes a last minute evening or weekend session is needed for a paper deadline, but that is unusual. We do allow users to come before we leave for the day and continue their session into the evening.
I do review photos occasionally from our users. Mostly I look at the images left on the SEM user interface and look deeper if I see signs of poor operation.
I plainly reference the cost of detectors during training and assure users that if they follow the standard operating procedures, they should have no problems. I also explain to them that the procedures need to be followed completely and in order. I worked hard to make the short form of the SOP short and practical. It is about 1-1/3 pages with another 2/3 page of common shortcut codes. And I tell them of some spectacular failures like ones that couldn't get an image and it was because they had failed to click the "Beam On" button on the UI.
I make it a point to refer users back to the written procedures. Otherwise I allow users to ask me too many questions and they never learn for themselves.
I hope these ideas help. I look forward to hearing what others have to say.
Warren Straszheim
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, April 10, 2015 6:09 PM To: Straszheim, Warren E [BIOTC]
X-from: roger.ristau-at-uconn.edu
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: Univ of Connecticut
Title-Subject: [Filtered] Access to User Facilities
Message: I am looking for advice from the community about how access to microscope facilities is granted to users. Specifically, I am thinking of something not quite so formal as the General User Access Proposal process available at National Labs, but not as open as our existing "free-for-all" where anyone who requests training can have open access.
I don't need advice on how to train users, rather, I need a fair process on how to determine who should or should not become a user.
Does anyone have a policy/process they wish to share--off line if you like--that weighs user requests? Perhaps a policy that includes various levels of access to users based on their needs and skills?
And a follow-up question is the magic "silver bullet" of how to monitor users after training to ensure that they are actually doing everything the way they were taught.
Thanks, Roger Ristau Univ of Connecticut
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Title-Subject: [Filtered] Access to user facilities
Message: I would say that it is important to identify those candidates which indeed have sufficient amount of work to do on SEM. Those who need 2-3 "nice pictures" to get are potentially problematic opertors with minor or zero motivation for learning and understanding the techique. The next issue is to provide the users for the approach or some signs of right way to analyse their samples. The idea is to assist users from the very beginning to find the right condition for imaging/microanalysis/diffraction pattern/etc and to explain the reasons for the choice. THe last important issue I would mention is interpersonal relations: it's better is the user (especially the new one with no experience) will feel safe and compfortable to report about any problem to the instrument/facility supervisor. Users always make mistakes, but to repair or to come back to te source of the mistake is always easier if you get the whole story and it is done ASAP. This is possible only if user does not afraid to report about a mistake. This also ensures that user will be instructed properly how to avoid this same mistake in the future. Good luck, Inna
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==============================Original Headers============================== 13, 17 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 11 09:17:25 2015 13, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3BEHPGs001406 13, 17 -- for {microscopy-at-microscopy.com} ; Sat, 11 Apr 2015 09:17:25 -0500 13, 17 -- Message-ID: {55292CF5.8010705-at-microscopy.com} 13, 17 -- Date: Sat, 11 Apr 2015 09:17:25 -0500 13, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 13, 17 -- MIME-Version: 1.0 13, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 17 -- Subject: viaWWW:Access to user facilities 13, 17 -- References: {201504110756.t3B7usBa029672-at-ns.microscopy.com} 13, 17 -- In-Reply-To: {201504110756.t3B7usBa029672-at-ns.microscopy.com} 13, 17 -- X-Forwarded-Message-Id: {201504110756.t3B7usBa029672-at-ns.microscopy.com} 13, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I operate our SEM/TEM/confocal/epi/laser microdissection facility just like Warren does, so he saved me a lot of typing! It mostly works.
Aloha, Tina
} Please send out a summary of replies once you get some. They can remain anonymous as far as I care. } } We are probably a small enough lab that we have remained rather informal about access. } } There are a few people that I have thought about redirecting away from the microscope as their brain just doesn't seem to be wired for that kind of work. I don't know if I have officially said anything out loud. Some seemed to have handed off SEM work to others in their research groups who are more adept. } } Upon granting sign-up privileges, I advise new users whether they are free to sign up as they please or whether I would definitely like to be nearby for their next few sessions. Our reservation system, ORS, provides the option for ones to be a resource monitor by e-mail. I set that option for myself so I get notified about every change in the schedule. Most notices can be ignored. A few users are on my watch list. The delete button is easy to use for those that don't concern me. } } We average about 25 hours of use per week. There is really no reason that ones HAVE to use the SEM outside of regular hours. A few users are granted or loaned keys for access at any time. Sometimes a last minute evening or weekend session is needed for a paper deadline, but that is unusual. We do allow users to come before we leave for the day and continue their session into the evening. } } I do review photos occasionally from our users. Mostly I look at the images left on the SEM user interface and look deeper if I see signs of poor operation. } } I plainly reference the cost of detectors during training and assure users that if they follow the standard operating procedures, they should have no problems. I also explain to them that the procedures need to be followed completely and in order. I worked hard to make the short form of the SOP short and practical. It is about 1-1/3 pages with another 2/3 page of common shortcut codes. And I tell them of some spectacular failures like ones that couldn't get an image and it was because they had failed to click the "Beam On" button on the UI. } } I make it a point to refer users back to the written procedures. Otherwise I allow users to ask me too many questions and they never learn for themselves. } } I hope these ideas help. I look forward to hearing what others have to say. } } Warren Straszheim } } -----Original Message----- } X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Friday, April 10, 2015 6:09 PM } To: Straszheim, Warren E [BIOTC] } Subject: [Microscopy] viaWWW:Access to User Facilities } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: roger.ristau-at-uconn.edu } } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both roger.ristau-at-uconn.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: roger.ristau-at-uconn.edu } Name: Roger Ristau } } Organization: Univ of Connecticut } } Title-Subject: [Filtered] Access to User Facilities } } Message: I am looking for advice from the community about how access to microscope facilities is granted to users. Specifically, I am thinking of something not quite so formal as the General User Access Proposal process available at National Labs, but not as open as our existing "free-for-all" } where anyone who requests training can have open access. } } I don't need advice on how to train users, rather, I need a fair process on how to determine who should or should not become a user. } } Does anyone have a policy/process they wish to share--off line if you like--that weighs user requests? Perhaps a policy that includes various levels of access to users based on their needs and skills? } } And a follow-up question is the magic "silver bullet" of how to monitor users after training to ensure that they are actually doing everything the way they were taught. } } Thanks, } Roger Ristau } Univ of Connecticut } } Login Host: 137.99.20.243 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } -- } =========================================== } Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. 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**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 22 -- From tina-at-pbrc.hawaii.edu Sun Apr 12 14:07:21 2015 5, 22 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3CJ7Kd5002835 5, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Apr 2015 14:07:21 -0500 5, 22 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 22 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id t3CJ7Ids015508 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 22 -- Sun, 12 Apr 2015 09:07:18 -1000 (HST) 5, 22 -- Received: from localhost (tina-at-localhost) 5, 22 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id t3CJ7Hh0015505; 5, 22 -- Sun, 12 Apr 2015 09:07:17 -1000 (HST) 5, 22 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 5, 22 -- Date: Sun, 12 Apr 2015 09:07:16 -1000 (HST) 5, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 22 -- X-X-Sender: tina-at-sf-v240 5, 22 -- To: roger.ristau-at-uconn.edu, Microscopy Listserver {Microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] RE: viaWWW:Access to User Facilities 5, 22 -- In-Reply-To: {201504110202.t3B22nwY030419-at-ns.microscopy.com} 5, 22 -- Message-ID: {Pine.GSO.4.64.1504120905230.15399-at-sf-v240} 5, 22 -- References: {201504110202.t3B22nwY030419-at-ns.microscopy.com} 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
I'm in an unusual environment: a makerspace or hackerspace in Chicago, which to my knowledge has the most public access policy of any SEM.
Any adult can join for $40/month which gets them 24x7 access to the SEM, as well as access to the rest of the tools in the space.
I can't assume any science background with people who want to use it. I've put together a 3 hour PowerPoint course, which is a prerequisite. Then I schedule about 1 1/2 hours of hands on training. Then they are free to use the scope without supervision.
Generally, there is some level of self selection because there is a time commitment to go through this. Still, I find that many people don't come back and use it. This is true of many of the tools at the space. People see a scarcity of training classes on, say, the milling machine or our large CNC router, and they sign up just in case, even if they don't have a project that could use it. We haven't found a good way around this problem, and with nearly 400 members, and all tool authorizations done by volunteers, it is a source of stress for the organization.
I have put in place a tiered access structure for the SEM, and right now have only implemented the bottom tier. This training doesn't permit users to change samples, use the sputter coater, critical point dryer, backscatter detector, or EDX. Users need to work with me to prep samples, so I can make sure nobody is going to put something wet or that will outgas in the chamber. Many of the users just want to see how a SEM works, so I keep interesting samples in the chamber at all times.
So far, the only user breakage problem I've had was someone who couldn't differentiate between first and second peak, and kept raising filament current until it blew. Not that big a deal.
That's also why I've been nervous about letting anyone do sample prep, risk running the sample into the BSD, or have a liquid nitrogen accident with the EDX. Also, our sputter coater is very finicky, and the Ar pressure difference between the plasma extinguishing and the power supply overloading is quite difficult to maintain with the needle valve. Additionally I may have been too hasty buying a CPD; we don't have a fume hood, so I'm not comfortable fixing wet samples in glutaraldehyde, and we've done absolutely nothing with wet samples. (I also don't have formal training myself, and I've been hoping to find a mentor in the Chicago area to help out.)
Cheers, Ryan I'm in an unusual environment: a makerspace or hackerspace in Chicago, which to my knowledge has the most public access policy of any SEM.
Any adult can join for $40/month which gets them 24x7 access to the SEM, as well as access to the rest of the tools in the space.
I can't assume any science background with people who want to use it. I've put together a 3 hour PowerPoint course, which is a prerequisite. Then I schedule about 1 1/2 hours of hands on training. Then they are free to use the scope without supervision.
Generally, there is some level of self selection because there is a time commitment to go through this. Still, I find that many people don't come back and use it. This is true of many of the tools at the space. People see a scarcity of training classes on, say, the milling machine or our large CNC router, and they sign up just in case, even if they don't have a project that could use it. We haven't found a good way around this problem, and with nearly 400 members, and all tool authorizations done by volunteers, it is a source of stress for the organization.
I have put in place a tiered access structure for the SEM, and right now have only implemented the bottom tier. This training doesn't permit users to change samples, use the sputter coater, critical point dryer, backscatter detector, or EDX. Users need to work with me to prep samples, so I can make sure nobody is going to put something wet or that will outgas in the chamber. Many of the users just want to see how a SEM works, so I keep interesting samples in the chamber at all times.
So far, the only user breakage problem I've had was someone who couldn't differentiate between first and second peak, and kept raising filament current until it blew. Not that big a deal.
That's also why I've been nervous about letting anyone do sample prep, risk running the sample into the BSD, or have a liquid nitrogen accident with the EDX. Also, our sputter coater is very finicky, and the Ar pressure difference between the plasma extinguishing and the power supply overloading is quite difficult to maintain with the needle valve. Additionally I may have been too hasty buying a CPD; we don't have a fume hood, so I'm not comfortable fixing wet samples in glutaraldehyde, and we've done absolutely nothing with wet samples. (I also don't have formal training myself, and I've been hoping to find a mentor in the Chicago area to help out.)
Cheers, Ryan
==============================Original Headers============================== 15, 20 -- From rdpierce-at-pobox.com Sun Apr 12 14:56:21 2015 15, 20 -- Received: from elna.mackenziegems.com (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) 15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3CJuKOL023140 15, 20 -- for {Microscopy-at-Microscopy.com} ; Sun, 12 Apr 2015 14:56:20 -0500 15, 20 -- Received: from [192.168.1.107] (108-219-222-230.lightspeed.cicril.sbcglobal.net [108.219.222.230]) 15, 20 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id 85DFB6E0EDD 15, 20 -- for {Microscopy-at-Microscopy.com} ; Sun, 12 Apr 2015 14:56:20 -0500 (CDT) 15, 20 -- Subject: Re: [Microscopy] viaWWW:Access to User Facilities 15, 20 -- References: {201504121929.t3CJTgSF019516-at-ns.microscopy.com} 15, 20 -- From: Ryan Pierce {rdpierce-at-pobox.com} 15, 20 -- Content-Type: text/plain; 15, 20 -- charset=us-ascii 15, 20 -- X-Mailer: iPad Mail (12F69) 15, 20 -- In-Reply-To: {201504121929.t3CJTgSF019516-at-ns.microscopy.com} 15, 20 -- Message-Id: {00622CF2-0689-436A-8F51-EFD902F21820-at-pobox.com} 15, 20 -- Date: Sun, 12 Apr 2015 14:56:18 -0500 15, 20 -- To: Microscopy-at-Microscopy.com 15, 20 -- Mime-Version: 1.0 (1.0) 15, 20 -- Content-Transfer-Encoding: 8bit 15, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3CJuKOL023140 ==============================End of - Headers==============================
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Email: chris.guerin-at-irc.vib-ugent.be Name: Chris Guerin
Organization: VIB
Title-Subject: [Filtered] Summer school microscopy in Ghent
Message: Hello Everyone:
I am pleased to announce that the 4th VIB Summer School in Advanced Light Microscopy will take place in the beautiful city of Ghent Belgium the week of June 15th. This course will give users both practical and theoretical background on the proper use of microscopes in biological research as well as introduce them to how to use imaging to obtain functional information from cells and tissues. The uses of advanced techniques such as FRET/FLIM, FRAP, TIRF, Super-resolution, and correlative light and electron microscopy will be explained and demonstrated. Hands on practical sessions will allow students to use and observe advanced microscope systems and to understand the best ways to design and carry out modern bioimaging. The final day will be dedicated to data analysis and interpretation of microscope generated data sets. This year the faculty of expert microscopists will include: Chris Guerin, VIB - Ghent University, Belgium Sebastian Munck, VIB - KU Leuven, Belgium Peter OÂToole, University of York, UK Bob Asselbergh, University of Antwerp, Belgium Stefan Vinckier, VIB - KU Leuven, Belgium Spencer Shorte, Institut Pasteur, France Lucy Collinson, London Research Institute, UK Andreas Schertel, Carl Zeiss, Germany Jean-Yves Tinevez, Institut Pasteur, France Eef Parthoens, VIB - Ghent University, Belgium You can see the full program and register at http://www.vib.be/en/training/research-training/courses/Pages/Summer-School-in-Advanced-Light-Microscopy.aspx. The course is limited to 32 participants and a simple application is required in case, (as in previous years), that the number of interested people exceeds our capacity. Registration is Â450 for the week long course and includes a daily lunch and dinner Mon, Tues and Thurs. evenings. We look forward to welcoming you to Ghent.
best wishes,
Chris GuĂŠrin Manager, VIB Bio Imaging Core Ghent
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 13 16:32:42 2015 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3DLWgna009165 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:32:42 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 7F06541616D 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:32:42 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at znl.com 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id EgcaxHBHO02R for {microscopy-at-microscopy.com} ; 13, 28 -- Mon, 13 Apr 2015 16:32:33 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 2B8B0416165 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:32:33 -0500 (CDT) 13, 28 -- Message-ID: {552C35F0.6000303-at-microscopy.com} 13, 28 -- Date: Mon, 13 Apr 2015 16:32:32 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:4th VIB Summer School in Advanced Light Microscopy in Ghent 13, 28 -- References: {201504131220.t3DCKcLX031307-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201504131220.t3DCKcLX031307-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201504131220.t3DCKcLX031307-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 13, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Kansas State University
Title-Subject: [Microscopy] viaWWW:Access to User Facilities
Message: Hi Roger, I have 6 years of experience in EM lab management. I was giving training to 5 user for TEM operation per semester. First step, I will define their real need of EM based on research activity and average usage of their research group.
1. I used to call user for 6 hours (2 hours per consecutive 3 days) for observation only. 2. One week step wise training (focusing, imaging, sample change, how to switch on-off machine). 3. Written test (10-15 MCQ on TEM operation) one assignment on SOP to operate TEM. (I never use to give them SOP, they have to make themselves and it has to approved by lab manager before use.) 4. Practical test on TEM by lab manager.
Permission to use TEM withdrawn, If any qualified user make three mistake (left the machine without following proper closing procedure, drastic change in alignment) and fail to report any incident or error.
Ravi.
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 13 16:33:35 2015 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3DLXZKJ009722 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:33:35 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id B7055416179 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:33:35 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at znl.com 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id c8FUrl-DkAX9 for {microscopy-at-microscopy.com} ; 16, 28 -- Mon, 13 Apr 2015 16:33:24 -0500 (CDT) 16, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id CAB7D416171 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:33:24 -0500 (CDT) 16, 28 -- Message-ID: {552C3624.2030403-at-microscopy.com} 16, 28 -- Date: Mon, 13 Apr 2015 16:33:24 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:Access to User Facilities 16, 28 -- References: {201504132047.t3DKlX0e008286-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201504132047.t3DKlX0e008286-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201504132047.t3DKlX0e008286-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Before you get to excited we are based in the UK in Bath and we would expect anyone who wants these items to collect them at their expense. I will give priority to people who want whole items but should any of you want parts let me know and if no takers for the whole thing then they are yours.
We have a Gatan Duo Ion Mill 600 diff pumped with sector and cryo cooler. It works but is getting tired so I will say 'spares or repair' It comes with a large assortment of consumables. If anyone want the whole thing then fine otherwise I am willing to offer the parts on first come first serve basis.
We also have a vintage dimple grinder and core drill. If these are any good to any of you then you are welcome to it but when I say vintage I mean 1981! it still works.
We also have some struers tenupol 2 systems again vintage but functional.
I look forward to hearing from you if you can save these from the scrap pile.
John
==============================Original Headers============================== 7, 26 -- From john.mitchels-at-gmail.com Tue Apr 14 13:28:44 2015 7, 26 -- Received: from mail-ig0-f176.google.com (mail-ig0-f176.google.com [209.85.213.176]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3EISi6h015033 7, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 13:28:44 -0500 7, 26 -- Received: by iget9 with SMTP id t9so80932978ige.1 7, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 11:28:43 -0700 (PDT) 7, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 26 -- d=gmail.com; s=20120113; 7, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 7, 26 -- bh=MQbIuyYuUj8Rc6+3sNJ921plKuY1BJ9svAIIJO76/lU=; 7, 26 -- b=HlJQYa1iaOdKLrw4JLYI4Vi9EzflN9eVKubCg/HWO6lE/JEKFI/jEDRCEF8EMUsLqs 7, 26 -- tyan9TgQff/YHJdNfe07saxLD0vGx9gYqYp5Kapwx9yyZwK7VsOalB0msEUyNXGbUfTR 7, 26 -- oSPb/e1PifHuiwbxBV/TgBVLtnshDPTsvd72rXJd2Bb+073iEmaTlQUG/L9u0Bo0Dn6V 7, 26 -- r9t3iD6G2flNj9GK9098Yqj0d/JAb6l/BrLbSzh1PF/jNkSKYOShA313j77wQb+/vJz5 7, 26 -- ThK4P5RxolSFdpmmKbZqx9519pwsiqtxP7xIkuL5DAbCIQUXEDIUcpNNb8+LH/UZSaZI 7, 26 -- 7RRg== 7, 26 -- MIME-Version: 1.0 7, 26 -- X-Received: by 10.50.43.162 with SMTP id x2mr26117609igl.46.1429036123732; 7, 26 -- Tue, 14 Apr 2015 11:28:43 -0700 (PDT) 7, 26 -- Received: by 10.36.17.19 with HTTP; Tue, 14 Apr 2015 11:28:43 -0700 (PDT) 7, 26 -- Date: Tue, 14 Apr 2015 19:28:43 +0100 7, 26 -- Message-ID: {CAHX7yTS=z+nw6vPOR_1WmC82HAP_7cwzthzeFXO9V7K7mqRdUw-at-mail.gmail.com} 7, 26 -- Subject: Free to good home Ion Mill, Dimple Grinder and Electro jet polishers (UK) 7, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
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Email: roy.geiss-at-colostate.edu Name: ROy Geiss
Organization: Colorado State University
Title-Subject: [Filtered] Summer School on Electron Diffraction
Message: Just a reminder that Wednesday, April 15 is the last day to register for the upcoming Summer School on Electron Diffraction at Colorado State University from May 19 to May 21, 2015. For a flyer, the program, and registration materials please go to: http://cif.colostate.edu/ and follow the links to the Summer school.
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 14 20:14:12 2015 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3F1ECt9012287 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 20:14:12 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 9402A4178AB 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 20:14:12 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at znl.com 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id CyLo1eoDc11w for {microscopy-at-microscopy.com} ; 14, 28 -- Tue, 14 Apr 2015 20:14:05 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 5776F4178A2 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 20:14:05 -0500 (CDT) 14, 28 -- Message-ID: {552DBB5C.2060407-at-microscopy.com} 14, 28 -- Date: Tue, 14 Apr 2015 20:14:04 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Summer School on Electron Diffraction 14, 28 -- References: {201504142339.t3ENd5AE010203-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201504142339.t3ENd5AE010203-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201504142339.t3ENd5AE010203-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: Are there any Canadian listers out there who are using an Edwards 1105 vacuum controller. I've bought one with gauges off ebay, but it doesn't have UL or CSA inspection stickers so the university won't approve me using it. If anyone has one in use a photo of the back with the stickers would go a long way towards getting it approved.
Thanks in advance for any help
Glenn
I do subscribe to the list but my institution insists on attaching advertising to the bottom of all my email, thus ensuring rejection by the very effective spam filters
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Apr 16 08:27:14 2015 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3GDRDgL004754 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 08:27:13 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 1E62C419687 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 08:27:13 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at znl.com 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id TvVtrwGxI2us for {microscopy-at-microscopy.com} ; 13, 28 -- Thu, 16 Apr 2015 08:27:05 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id BFB5941967B 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 08:27:05 -0500 (CDT) 13, 28 -- Message-ID: {552FB8A9.2030207-at-microscopy.com} 13, 28 -- Date: Thu, 16 Apr 2015 08:27:05 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Edwards 1105 vacuum controller 13, 28 -- References: {201504161322.t3GDMfUi004706-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201504161322.t3GDMfUi004706-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201504161322.t3GDMfUi004706-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We would like to bring your attention to the workshop "Big, Deep, and Smart Data Analytics in Materials Imaging" organized jointly by the five DOE Nanoscale Science Research Centers (Program Committee: E. Stach, J. Werner, D. Miller, S.V. Kalinin and J. Schuck) and to be held at Oak Ridge National Laboratory on June 8-10. This workshop will bring together researchers from different imaging disciplines (electron microscopy, scanning probe microscopy, focused x-ray, neutron, atom probe tomography, chemical imaging, optical microscopies) as well as experts in mathematical/statistical/computational approaches to discuss opportunities and future needs in the integration of advanced data analytics and theory into imaging science. It will provide a forum to present achievements in the various imaging disciplines with emphasis on acquisition, visualization, and analysis of multidimensional data sets, the corresponding approaches for theory-experiment matching, and novel opportunities for instrumental development enabled by the availability of high speed data analytic tools. Additional information regarding this workshop will be posted at the http://www.cnms.ornl.gov/JointNSRC2015/.
Please address questions to Amanda Zetans, zetansac-at-ornl.gov {mailto:zetansac-at-ornl.gov} , 865.241.1182.
On behalf of the organizers,
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Theme Leader, Center for Nanophase Materials Science
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 10, 23 -- From sergei2-at-ornl.gov Thu Apr 16 16:21:37 2015 10, 23 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3GLLbv9008227 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 16:21:37 -0500 10, 23 -- X-SG: RELAYLIST 10, 23 -- X-IronPort-AV: E=Sophos;i="5.11,590,1422939600"; 10, 23 -- d="scan'208";a="78488049" 10, 23 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 10, 23 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 16 Apr 2015 17:21:37 -0400 10, 23 -- Received: from [128.219.192.92] (pc83680.ornl.gov [128.219.192.92]) 10, 23 -- (using TLSv1 with cipher DHE-RSA-CAMELLIA256-SHA (256/256 bits)) 10, 23 -- (No client certificate requested) 10, 23 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 275D3E01F4 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 17:21:37 -0400 (EDT) 10, 23 -- Message-ID: {553027E0.8040803-at-ornl.gov} 10, 23 -- Date: Thu, 16 Apr 2015 17:21:37 -0400 10, 23 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 10, 23 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:14.0) Gecko/20120713 Thunderbird/14.0 10, 23 -- MIME-Version: 1.0 10, 23 -- To: microscopy-at-microscopy.com 10, 23 -- Subject: Workshop - Big, Deep, and Smart Data Analytics in Materials Imaging 10, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: susan.vanhorn-at-stonybrook.edu Name: Susan C Van Horn
Organization: Central Microscopy Imaging Center
Title-Subject: [Filtered] embedding yeast
Message: I always have problems embedding/infiltrating yeast!!! have tried different resins, vacuum steps,etc.. does anyone have an embedding protocol/resin that works??!!! thanks for sharing sue
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Apr 17 07:29:33 2015 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3HCTXEL027358 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:33 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 440F341AA1F 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:33 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at znl.com 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id M6SZ3DeJ6q02 for {microscopy-at-microscopy.com} ; 11, 28 -- Fri, 17 Apr 2015 07:29:21 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 64DA441AA14 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:21 -0500 (CDT) 11, 28 -- Message-ID: {5530FCA1.8000603-at-microscopy.com} 11, 28 -- Date: Fri, 17 Apr 2015 07:29:21 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:embedding yeast 11, 28 -- References: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: Pruessner-at-ukzn.ac.za Name: Karin Pruessner
Organization: University of KwaZulu-Natal, Durban
Title-Subject: [Filtered] Postdoctoral poasition in energy materials
Message: The University of KwaZulu-Natal in Durban, South Africa, is home to an interdisciplinary Nanotechnology Platform consisting of 4 pillars in Nano Energy, Nano Materials, Nano Health and Quantum Physics. The Nano Energy pillar has at least one, possibly two, positions available for a Postdoctoral Researcher to be filled as soon as possible. Our team consists of Chemists, Physicists, Materials Scientists, and Engineers. We are working on materials for energy applications, specifically on the development of an off-grid refrigeration unit for rural areas in Africa. We are looking for a Materials Scientist with a solid background in Materials Characterization to join us. Neighboring fields will be considered. Hands-on experience in Scanning and Transmission Electron Microscopy, X-Ray Diffraction, and Raman Spectroscopy is expected. Experience in at least one of the following areas would be beneficial:  Nano Photovoltaics  Nano Energy Storage  Nano Cooling Fluids  Graphene The university houses an Electron Microscopy Unit. XRD and Raman Spectroscopy are available in the School of Chemistry and Physics. Advanced instrumentation including aberration-corrected HRTEM is available through national user centers. Interested candidates should send their electronic application materials to: Dr Karin Pruessner UKZN Nanotechnology Platform, Nano Energy Coordinator School of Chemistry and Physics University of KwaZulu-Natal - Westville Campus University Road Durban, 4000 South Africa Ph: ++27 31 260-7660 e-mail: Pruessner-at-ukzn.ac.za
Review of applications will begin immediately and continue until the posts are filled.
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Not sure what type of yeast you work with. We work with budding yeast and have been had good luck with microwave radiation, especially for stationary yeast, of which the cell wall is very tough. http://www.ncbi.nlm.nih.gov/pubmed/17156022
For log-phase cells, extended infiltration (at least one overnight) works fine using Spurr. I believe the low viscosity of Spurr helps.
Best luck and let me know if you need a reprint.
Zhaojie Zhang University of Wyoming
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, April 17, 2015 6:39 AM To: Z.J. Zhang
X-from: susan.vanhorn-at-stonybrook.edu
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Email: susan.vanhorn-at-stonybrook.edu Name: Susan C Van Horn
Organization: Central Microscopy Imaging Center
Title-Subject: [Filtered] embedding yeast
Message: I always have problems embedding/infiltrating yeast!!! have tried different resins, vacuum steps,etc.. does anyone have an embedding protocol/resin that works??!!! thanks for sharing sue
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Apr 17 07:29:33 2015 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3HCTXEL027358 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:33 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 440F341AA1F 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:33 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at znl.com 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id M6SZ3DeJ6q02 for {microscopy-at-microscopy.com} ; 11, 28 -- Fri, 17 Apr 2015 07:29:21 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 64DA441AA14 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:21 -0500 (CDT) 11, 28 -- Message-ID: {5530FCA1.8000603-at-microscopy.com} 11, 28 -- Date: Fri, 17 Apr 2015 07:29:21 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:embedding yeast 11, 28 -- References: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
dear Sue, there are many resources in the web concerning the (tricky) task to properly embed yeast cells for ultrathin sectioning. my favourite ones, as of today: Mary's manual (Boulder, CO, USA): http://bio3d.colorado.edu/docs/mmanual.pdf then Giddings, T. H., Jr., OâToole, E. T., Morphew, M., Mastronarde, D. N., McIntosh, J. R., and Winey, M. (2001). Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis. Methods Cell Biol. 67, 27â42 (yes, Mary is one of the co-authors), and Kent L McDonald - Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material, -- Protoplasma (2014) 251:429â448 DOI 10.1007/s00709-013-0575-y. This article is quite helpful as it shows that some of the paradigms of 'old' embedding protocols are clearly out-dated, if not to say "wrong". It also depends on your equipment, the specific question, and so on. kind regards - reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: http://www.mc2015.de DGE - Microscopy Conference 2015, Sept 6-11, GĂśttingen http://www.emc2016.fr/en/ 16th Europ Microsc Congress EMC 2016 in Lyon, FR next Microbiol. conferences: VAAM - Annual Conf March 13-16, 2016, Jena
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Hi folks, I am acquiring a Jeol JSM-T200 SEM and wanted to try and dig up some info on it, or similar models. Mainly interested in the raster scanning circuitry, as this seems to need some work or possibly be re-implemented from scratch.
Any info would be helpful, at this point I've acquired the user-manual... so I guess I'm looking for the service-manual now.
I'll even take info on similar models, since that is likely better than having no clues at all!
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Email: martin.roe-at-nottingham.ac.uk Name: Martin Roe
Organization: Nottingham University
Title-Subject: [Filtered] JEOL 6400 SEM Diffusion pump heater element
Message: Dear Listservers, Does anyone have a spare diffusion pump heater element for a JEOL 6400 SEM? There are two diff pumps on the instrument and it is the larger of the two that has the heater problem. The original part number of the heater is 322000025 and is 4 inches in diameter (rated at 200V/600W) with what seemed like ÂTKÂ written on it. Would be willing to pay for the part and shipment of it. Thanks, Martin
Martin Roe Department of Mechanical, Materials & Manufacturing Faculty of Engineering University of Nottingham, Wolfson Building, University Park, Nottingham, NG7 2RD, UK
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NIST DTSA-II has recently been updated to version Iona. (Download for free from http://www.cstl.nist.gov/div837/837.02/epq/dtsa2/index.html) DTSA-II provides a host of tools for quantitative EDS microanalysis including quantification, simulation and measurement planning. Iona has a host of improvements both large and small which are detailed in the release notes on the web site.
Further details on quantitative analysis with NIST DTSA-II are available in Newbury & Ritchie's J. Mat Sc. Article "Performing elemental microanalysis with high accuracy and high precision by scanning electron microscopy/silicon drift detector energy-dispersive X-ray spectrometry (SEM/SDD-EDS)" (free for download from http://link.springer.com/article/10.1007/s10853-014-8685-2 ). This article demonstrates the potential of the modern EDS detector to perform reliable, quantitatively accurate compositional measurements even for some very challenging samples.
==============================Original Headers============================== 5, 42 -- From nicholas.ritchie-at-nist.gov Tue Apr 21 12:40:33 2015 5, 42 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1bon0117.outbound.protection.outlook.com [157.56.111.117]) 5, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3LHeXfe002896 5, 42 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2015 12:40:33 -0500 5, 42 -- Received: from BN3PR09MB0595.namprd09.prod.outlook.com (25.160.119.28) by 5, 42 -- BN3PR09MB0595.namprd09.prod.outlook.com (25.160.119.28) with Microsoft SMTP 5, 42 -- Server (TLS) id 15.1.136.25; Tue, 21 Apr 2015 17:40:32 +0000 5, 42 -- Received: from BN3PR09MB0595.namprd09.prod.outlook.com ([25.160.119.28]) by 5, 42 -- BN3PR09MB0595.namprd09.prod.outlook.com ([25.160.119.28]) with mapi id 5, 42 -- 15.01.0136.026; Tue, 21 Apr 2015 17:40:32 +0000 5, 42 -- From: "Ritchie, Nicholas" {nicholas.ritchie-at-nist.gov} 5, 42 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 42 -- Subject: NIST DTSA-II Iona released & high precision quant with an SDD 5, 42 -- Thread-Topic: NIST DTSA-II Iona released & high precision quant with an SDD 5, 42 -- Thread-Index: AdB8WH/3zCY7ftpPRVWY5nHI2M73bw== 5, 42 -- Date: Tue, 21 Apr 2015 17:40:31 +0000 5, 42 -- Message-ID: {BN3PR09MB05958089BF34F4467D081439F1EF0-at-BN3PR09MB0595.namprd09.prod.outlook.com} 5, 42 -- Accept-Language: en-US 5, 42 -- Content-Language: en-US 5, 42 -- X-MS-Has-Attach: 5, 42 -- X-MS-TNEF-Correlator: 5, 42 -- authentication-results: spf=none (sender IP is ) 5, 42 -- smtp.mailfrom=nicholas.ritchie-at-nist.gov; 5, 42 -- x-originating-ip: [129.6.126.223] 5, 42 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BN3PR09MB0595; 5, 42 -- x-forefront-antispam-report: BMV:1;SFV:NSPM;SFS:(10019020)(979002)(6009001)(11905935001)(77156002)(40100003)(2656002)(50986999)(15188555004)(54356999)(15975445007)(102836002)(74316001)(46102003)(86362001)(87936001)(122556002)(19580395003)(2501003)(450100001)(107886001)(92566002)(229853001)(2900100001)(76576001)(66066001)(99286002)(33656002)(110136001)(2351001)(62966003)(969003)(989001)(999001)(1009001)(1019001);DIR:OUT;SFP:1102;SCL:1;SRVR:BN3PR09MB0595;H:BN3PR09MB0595.namprd09.prod.outlook.com;FPR:;SPF:None;MLV:ovrnspm;PTR:InfoNoRecords;LANG:en; 5, 42 -- x-microsoft-antispam-prvs: {BN3PR09MB059526675199707646AF126BF1EF0-at-BN3PR09MB0595.namprd09.prod.outlook.com} 5, 42 -- x-exchange-antispam-report-test: UriScan:; 5, 42 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5002010)(5005006);SRVR:BN3PR09MB0595;BCL:0;PCL:0;RULEID:;SRVR:BN3PR09MB0595; 5, 42 -- x-forefront-prvs: 0553CBB77A 5, 42 -- received-spf: None (protection.outlook.com: nist.gov does not designate 5, 42 -- permitted sender hosts) 5, 42 -- Content-Type: text/plain; charset="us-ascii" 5, 42 -- MIME-Version: 1.0 5, 42 -- X-OriginatorOrg: nist.gov 5, 42 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 21 Apr 2015 17:40:31.7353 5, 42 -- (UTC) 5, 42 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 5, 42 -- X-MS-Exchange-CrossTenant-id: 2ab5d82f-d8fa-4797-a93e-054655c61dec 5, 42 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN3PR09MB0595 5, 42 -- Content-Transfer-Encoding: 8bit 5, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3LHeXfe002896 ==============================End of - Headers==============================
A colleague has an old JEOL JAM-7830 Auger microprobe. He has had troubles starting it back up after the last couple power outages. The system fails to recognize the field emission gun and instead thinks it has a LaB6 source.
He has been able to stumble around and eventually get it to work by trying various things, but the results are not immediately obvious and he is not clear which steps have been effective and necessary or if the order of steps is important.
Does anyone have experience with this problem on a 7830 and have some suggestions for him? We thank you in advance.
Warren Straszheim Ames Lab/Iowa State University
==============================Original Headers============================== 5, 40 -- From wesaia-at-iastate.edu Tue Apr 21 18:03:17 2015 5, 40 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2on0095.outbound.protection.outlook.com [207.46.100.95]) 5, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3LN3G9p011221 5, 40 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2015 18:03:16 -0500 5, 40 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 5, 40 -- BLUPR04MB548.namprd04.prod.outlook.com (10.141.29.152) with Microsoft SMTP 5, 40 -- Server (TLS) id 15.1.136.25; Tue, 21 Apr 2015 23:03:15 +0000 5, 40 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.116]) by 5, 40 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.116]) with mapi id 5, 40 -- 15.01.0136.014; Tue, 21 Apr 2015 23:03:15 +0000 5, 40 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 5, 40 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 40 -- Subject: Problem with Auger gun startup 5, 40 -- Thread-Topic: Problem with Auger gun startup 5, 40 -- Thread-Index: AdB8hoYj/rAzWfGiRNCIvyhA2Vmi7Q== 5, 40 -- Date: Tue, 21 Apr 2015 23:03:15 +0000 5, 40 -- Message-ID: {BLUPR04MB546DE08E1D8AC17743D80CFD7EF0-at-BLUPR04MB546.namprd04.prod.outlook.com} 5, 40 -- Accept-Language: en-US 5, 40 -- Content-Language: en-US 5, 40 -- X-MS-Has-Attach: 5, 40 -- X-MS-TNEF-Correlator: 5, 40 -- authentication-results: microscopy.com; dkim=none (message not signed) 5, 40 -- header.d=none; 5, 40 -- x-originating-ip: [129.186.227.4] 5, 40 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BLUPR04MB548; 5, 40 -- x-microsoft-antispam-prvs: {BLUPR04MB548B39B6B4CA182CF79CA7BD7EF0-at-BLUPR04MB548.namprd04.prod.outlook.com} 5, 40 -- x-forefront-antispam-report: BMV:1;SFV:NSPM;SFS:(10009020)(6009001)(504964003)(99286002)(40100003)(2351001)(76576001)(107886001)(86362001)(90282001)(2900100001)(450100001)(110136001)(2501003)(33656002)(92566002)(75432002)(74316001)(54356999)(50986999)(2656002)(87936001)(122556002)(62966003)(88552001)(229853001)(89122001)(46102003)(66066001)(102836002)(77156002);DIR:OUT;SFP:1101;SCL:1;SRVR:BLUPR04MB548;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 5, 40 -- x-exchange-antispam-report-test: UriScan:; 5, 40 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5005006)(5002010);SRVR:BLUPR04MB548;BCL:0;PCL:0;RULEID:;SRVR:BLUPR04MB548; 5, 40 -- x-forefront-prvs: 0553CBB77A 5, 40 -- Content-Type: text/plain; charset="us-ascii" 5, 40 -- MIME-Version: 1.0 5, 40 -- X-OriginatorOrg: iastate.edu 5, 40 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 21 Apr 2015 23:03:15.6208 5, 40 -- (UTC) 5, 40 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 5, 40 -- X-MS-Exchange-CrossTenant-id: 0347d89a-0174-4dd3-adeb-3339c89c35f5 5, 40 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BLUPR04MB548 5, 40 -- Content-Transfer-Encoding: 8bit 5, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3LN3G9p011221 ==============================End of - Headers==============================
I find myself in a little bit of a quandary. ARDL would like to upgrade our low to medium light imaging capacity. One of the quandaries is the fellow with the purse strings has demands. He wants a simple imaging system with the highest resolution at a reasonable price.
I'm looking for a stereomicroscope with a good, flat field, corrected lens for color and aberration. A course and fine focus would be well received as would a translumination base, but these are not deal breakers. I'd like a camera with about 5-12 megapixals. I'd like software with some basic annotating features, image processing ability and measurement functions. The software should have file storage system suitable for both scientific and legal applications.
If you're a vendor, if you have experience with these systems, if you have an axe to grind or just want to get in on the conversation please get in touch with me. I'd like to get demos if possible.
It's been suggest we are in a rush to spend money, but......I understand the reputation we have with the closed purse. I don't think I have to say anything else.
Thank you!
Frank Karl ARDL Frank_karl-at-ARDL.com 330-794-6600
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 10, 28 -- From frank_karl-at-ardl.com Thu Apr 23 13:02:41 2015 10, 28 -- Received: from cal1-mh775.smtproutes.com (cal1-mh775.smtproutes.com [208.70.91.141]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3NI2ePp031802 10, 28 -- for {microscopy-at-microscopy.com} ; Thu, 23 Apr 2015 13:02:41 -0500 10, 28 -- X-Katharion-ID: 1429812143.35221.cal1-mh775 10, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by 10, 28 -- cal1-mh775.smtproutes.com [(192.69.16.141)] with ESMTP via TCP 10, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 23 Apr 2015 18:02:23 +0000 10, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 10, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Thu, 23 10, 28 -- Apr 2015 14:02:04 -0400 10, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 10, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 10, 28 -- {microscopy-at-microscopy.com} 10, 28 -- Date: Thu, 23 Apr 2015 14:02:03 -0400 10, 28 -- Subject: Stereomicroscope and cameria 10, 28 -- Thread-Topic: Stereomicroscope and cameria 10, 28 -- Thread-Index: AdB975qsNmEk30WkQ4eftJNgFUeMbQ== 10, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772CA5D9EB69-at-exchange2k7.ad.ardl.com} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3NI2ePp031802 ==============================End of - Headers==============================
Re: Microscope - I'm sure that a number of vendors will contact you Re: Camera + SW - have forwarded your request to a colleague who specializes in that area and has some clever solution
One thing to consider which is economical and also dramatically expands your stereomicroscopy is the fluorescence system from Nightsea (Nightsea.com ... Caveat: No financial interest). It has been interesting to see the response at a variety of meetings I've attended over the last 6 months. Although fluorescence came up through the ranks via the Life Sciences, there are intriguing applications in materials sciences as well. I remember looking at pockets/bubbles in an elastomer to determine if they had air or gel. As I remember, the gel fluoresced. Also great when used with fluorescent dyes for imaging surface cracks/defects. Nightsea's inventor, Dr. Charlie Mazel, is always interested in looking at new things. I recommend giving him a call and sending him some samples to see if this should be part of your planned upgrade.
Hope this was helpful.
Good hunting! Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education ... "Education, not Training" 7101 Royal Glen Trail, Suite A - McKinney, TX 75070 - P: 972-924-5310 www.MicroscopyEducation.com
NEW! Getting involved in Raman or FTIR? MME is now offering courses in these areas specifically for microscopists! Now scheduling courses through the end of 2015. We can customize a course on nearly any topic, from fluorescence to confocal to image analysis to SEM/TEM. Call today for a free training evaluation.
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==============================Original Headers============================== 23, 29 -- From bfoster-at-the-mip.com Thu Apr 23 17:42:58 2015 23, 29 -- Received: from barcelona.directrouter.com (barcelona.directrouter.com [72.249.98.64]) 23, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3NMgvBL004553 23, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 23 Apr 2015 17:42:57 -0500 23, 29 -- Message-Id: {201504232242.t3NMgvBL004553-at-ns.microscopy.com} 23, 29 -- Received: from 99-168-104-53.lightspeed.rcsntx.sbcglobal.net ([99.168.104.53]:60683 helo=Bs-PC.the-mip.com) 23, 29 -- by barcelona.directrouter.com with esmtpsa (TLSv1:DHE-RSA-AES256-SHA:256) 23, 29 -- (Exim 4.85) 23, 29 -- (envelope-from {bfoster-at-the-mip.com} ) 23, 29 -- id 1YlPpc-0007kB-DA; Thu, 23 Apr 2015 17:42:52 -0500 23, 29 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 23, 29 -- Date: Thu, 23 Apr 2015 17:42:48 -0500 23, 29 -- To: frank_karl-at-ardl.com, 23, 29 -- "MIcroscopy-Microscopy.com" {Microscopy-at-Microscopy.com} 23, 29 -- From: Barbara Foster {bfoster-at-the-mip.com} 23, 29 -- Subject: Re: [Microscopy] Stereomicroscope and cameria 23, 29 -- In-Reply-To: {201504231816.t3NIGN6w012025-at-ns.microscopy.com} 23, 29 -- References: {201504231816.t3NIGN6w012025-at-ns.microscopy.com} 23, 29 -- Mime-Version: 1.0 23, 29 -- Content-Type: text/plain; charset="us-ascii" 23, 29 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 23, 29 -- X-AntiAbuse: Primary Hostname - barcelona.directrouter.com 23, 29 -- X-AntiAbuse: Original Domain - microscopy.com 23, 29 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 23, 29 -- X-AntiAbuse: Sender Address Domain - the-mip.com 23, 29 -- X-Get-Message-Sender-Via: barcelona.directrouter.com: authenticated_id: bfoster-at-the-mip.com 23, 29 -- X-Source: 23, 29 -- X-Source-Args: 23, 29 -- X-Source-Dir: ==============================End of - Headers==============================
Does anyone have a spare diode holder (preferably with diode) for the GW 113A backscatter detector?
This is for an S-800 that I have been restoring for the past year for personal / educational use. I have the GW arm, the 113A electronics, and the external beam monitor electronics (for compensate for the cold field emission tip).
Any help in sourcing the diode holder / diode would be appreciated. Even drawings of this diode holder would be helpful since I could always make one up!
Hello, We are seeking a Microscopy Facility Specialist here at the Johns Hopkins School of Medicine Microscope Facility, Baltimore MD. The main focus is on fluorescence and confocal microscopy operation, training and maintenance, with emphasis on computer based analysis. All interested parties should apply at: https://hrnt.jhu.edu/jhujobs/job_view.cfm?view_req_id=64229&view=sch
We look forward to reading your resumes.
Sincerely, Michael Delannoy Assoc. Director JHU SOM Microscope Facility
The University of Bath's Microscopy and Analysis Facility (in the UK) is looking for a microscopy specialist to look after users from chemistry, physics and engineering. The job is advertised at the following link. Note the deadline is the 10th May with interviews on the 18th ideally. The role will be to look after the suite collection of electron microscopes. Candidates with experience of maintenance of older equipment, elemental analysis, diffraction analysis and if possible Raman, FTIR, and SPM knowledge.
This link is for the job: http://www.bath.ac.uk/jobs/Vacancy.aspx?ref=SS3127
This link is for the suite: http://www.bath.ac.uk/facilities/mas
==============================Original Headers============================== 4, 26 -- From john.mitchels-at-gmail.com Mon Apr 27 11:19:33 2015 4, 26 -- Received: from mail-ie0-f175.google.com (mail-ie0-f175.google.com [209.85.223.175]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3RGJXHM028015 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 11:19:33 -0500 4, 26 -- Received: by iedfl3 with SMTP id fl3so147192867ied.1 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 09:19:32 -0700 (PDT) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=20120113; 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 26 -- bh=/YKIbA1sQJo4uBt8nJfMT2OyAlGoxSm7DdSiFNViElc=; 4, 26 -- b=PRvhpnXBEpZIOwBAtY2stimWb+N/surS6OICZ5GabW5OxL2rNXegnh82IDCIxDfvb0 4, 26 -- nEZFzw7WIOGCpTG1OlmQ/+9S4ePYDYb2JYBJrS7Q+exWNflREAomF7xnimBAkaGPKW8K 4, 26 -- 1TJ2M3v/NVol8i0rNwPjCqkJLfKxEtW4s0SqAtcleK7SJM4lGtzI5SP/6E8QR1J8OgqF 4, 26 -- EFzP25uqubnQyuSt9S60tm1hVOdSg0L6fGIYZS/7FJJPf2+5EGmBEZZSAmtJY5UHb9dD 4, 26 -- h/6/wttRsKkxH+xsnLfbm9IxnceMfMrwE8ApQEb0eW43ZucMIl+gWFwKnA9+hHHbqx83 4, 26 -- 5GYw== 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Received: by 10.107.157.70 with SMTP id g67mr15199886ioe.11.1430151572547; 4, 26 -- Mon, 27 Apr 2015 09:19:32 -0700 (PDT) 4, 26 -- Received: by 10.36.17.19 with HTTP; Mon, 27 Apr 2015 09:19:32 -0700 (PDT) 4, 26 -- Date: Mon, 27 Apr 2015 17:19:32 +0100 4, 26 -- Message-ID: {CAHX7yTTTwr4qRPGUDLeuPnTv3aZ75y_9t5thqDfDvJMrK8XOqw-at-mail.gmail.com} 4, 26 -- Subject: Materials Microscopy Specialist Position Bath, UK 4, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
I would like to revisit the problem of old software, computers, and institutional support.
We have many instruments that run on proprietary software that has not been upgraded to more modern operating systems.
For example, some of our instruments use programs only compatible with Windows XP.
Our IT guys want to 'upgrade' all campus computers to a newer operating system and don't want any old machines running.
Is it reasonable to tell them that we need our old XP (and earlier) computers to keep our instruments running.
What would be a good approach to satisfy their urge to stay current and our need to live in the past?
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 20, 38 -- From prvs=155290bd1d=jkrupp-at-deltacollege.edu Mon Apr 27 15:15:58 2015 20, 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.175.111]) 20, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t3RKFvvC020511 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 15:15:58 -0500 20, 38 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 20, 38 -- by localhost (Email Security Appliance) with SMTP id 3D82F342714_53E98F9B 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 20:15:53 +0000 (GMT) 20, 38 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) 20, 38 -- by mailin.deltacollege.edu (Sophos Email Appliance) with SMTP id 330D72FD6F1_53E98F9F 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 20:15:53 +0000 (GMT) 20, 38 -- Received: from localhost (localhost [127.0.0.1]) 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C13C6FE5C545 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 20, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 20, 38 -- with ESMTP id IJghSG1cqNe5 for {Microscopy-at-microscopy.com} ; 20, 38 -- Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- Received: from localhost (localhost [127.0.0.1]) 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5DFC2FE5C547 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 20, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 20, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 20, 38 -- with ESMTP id 4rHh9uhMGHZx for {Microscopy-at-microscopy.com} ; 20, 38 -- Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- Received: from jonathans-mbp.deltacollege.edu (unknown [10.146.0.14]) 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 41E6FFE5C545 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 20, 38 -- Content-Type: text/plain; charset=us-ascii 20, 38 -- Subject: Computer updates 20, 38 -- Date: Mon, 27 Apr 2015 13:15:53 -0700 20, 38 -- Message-Id: {B7150470-C9EA-44A4-B8DC-762A5F824F24-at-deltacollege.edu} 20, 38 -- To: Microscopy-at-microscopy.com 20, 38 -- Mime-Version: 1.0 (Apple Message framework v1085) 20, 38 -- X-Mailer: Apple Mail (2.1085) 20, 38 -- Content-Transfer-Encoding: 8bit 20, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3RKFvvC020511 ==============================End of - Headers==============================
I think all of us feel your pain. Having done both microscopy and systems administration I empathize on both sides of the equation.
The IT staff often don't "get" that you have a valuable piece of scientific equipment that will continue to work for many years (and which people NEED to use for their education or research interests), but will never again run an up-to-date operating system. Contrary to popular belief, you aren't being a Luddite, you are stuck with something that will break if the OS is upgraded and you can't afford to break it or replace it with a newer piece of equipment.
X-from the IT perspective they are looking at a computer that will no longer receive security patches and whose antivirus support has already or is about to run out. Understandably, they want it off their network.
With even flash drives being capable of transmitting viruses, you need a way for people to use the equipment, but not have a way for the computer to become infected.
You might also need backup hardware that can still run Windows XP (some labs at the University here have a small stockpile of XP compatible computers as fallbacks). At minimum you should consider regularly creating disk images of the hard drive(s) to ensure that you can recreate the setup when some of the hardware fails (it's not getting any younger). Old hardware drivers can be difficult to find, especially if they came on manufacturer's CDs or floppies that you may or may not be able to locate in a crisis.
What some labs have done is create a private network with a file server. The file server uses a current/secure OS and is where users on all the old computers store their files. The server can share the files out to the larger network via one connection, while firewalling the private network (where all the WinXP, Win2K, etc computers live) that the server is connected to via a different connection. In other words. The file server has two network cards. Users can no longer use floppies or USB devices on the old computers, they can only physically connect to the server.
Depending on how your building wiring was done, the IT folks may be able to create the private network using the building's network switch, without the need for physical rewiring.
There may be other ways, but this seems like the most viable to me. It will take some money and expertise to pull off, but the alternative is worse...
Doug
------------------------------------------------------------------------------------------ Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
http://swehsc.pharmacy.arizona.edu/micro Home of: "Microscopy and Imaging Resources on the WWW"
UA Microscopy Alliance - http://microscopy.arizona.edu
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Monday, April 27, 2015 1:16 PM To: Cromey, Douglas W - (dcromey)
Dear all:
I would like to revisit the problem of old software, computers, and institutional support.
We have many instruments that run on proprietary software that has not been upgraded to more modern operating systems.
For example, some of our instruments use programs only compatible with Windows XP.
Our IT guys want to 'upgrade' all campus computers to a newer operating system and don't want any old machines running.
Is it reasonable to tell them that we need our old XP (and earlier) computers to keep our instruments running.
What would be a good approach to satisfy their urge to stay current and our need to live in the past?
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 20, 38 -- From prvs=155290bd1d=jkrupp-at-deltacollege.edu Mon Apr 27 15:15:58 2015 20, 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.175.111]) 20, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t3RKFvvC020511 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 15:15:58 -0500 20, 38 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 20, 38 -- by localhost (Email Security Appliance) with SMTP id 3D82F342714_53E98F9B 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 20:15:53 +0000 (GMT) 20, 38 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) 20, 38 -- by mailin.deltacollege.edu (Sophos Email Appliance) with SMTP id 330D72FD6F1_53E98F9F 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 20:15:53 +0000 (GMT) 20, 38 -- Received: from localhost (localhost [127.0.0.1]) 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C13C6FE5C545 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 20, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 20, 38 -- with ESMTP id IJghSG1cqNe5 for {Microscopy-at-microscopy.com} ; 20, 38 -- Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- Received: from localhost (localhost [127.0.0.1]) 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5DFC2FE5C547 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 20, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 20, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 20, 38 -- with ESMTP id 4rHh9uhMGHZx for {Microscopy-at-microscopy.com} ; 20, 38 -- Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- Received: from jonathans-mbp.deltacollege.edu (unknown [10.146.0.14]) 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 41E6FFE5C545 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 20, 38 -- Content-Type: text/plain; charset=us-ascii 20, 38 -- Subject: Computer updates 20, 38 -- Date: Mon, 27 Apr 2015 13:15:53 -0700 20, 38 -- Message-Id: {B7150470-C9EA-44A4-B8DC-762A5F824F24-at-deltacollege.edu} 20, 38 -- To: Microscopy-at-microscopy.com 20, 38 -- Mime-Version: 1.0 (Apple Message framework v1085) 20, 38 -- X-Mailer: Apple Mail (2.1085) 20, 38 -- Content-Transfer-Encoding: 8bit 20, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3RKFvvC020511 ==============================End of - Headers==============================
==============================Original Headers============================== 40, 37 -- From dcromey-at-email.arizona.edu Mon Apr 27 15:50:11 2015 40, 37 -- Received: from mails1n1-route0.email.arizona.edu (mails1n1-route0.email.arizona.edu [128.196.130.51]) 40, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3RKoA0d008874 40, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 15:50:10 -0500 40, 37 -- X-IronPort-Anti-Spam-Filtered: true 40, 37 -- X-IronPort-Anti-Spam-Result: A2GWAwDXnz5V/4Y1jApZA4MMU1wFxVSCNwqGAAICAoE1PBABAQEBAQEBA4EHgk86AQgEHQITKxICGQEBAQQBAQE3FB0aBAIBBQMRAwEBAQ0SCQcxARQDAQUIAgQKCQgOBwSICg3CW4RsAQEBAQEBAQMBAQEBAQEBARqLOIJ0AYEkCRACAQUJFxsSBQYLghxNgTMFhSyBFosTgy5ThjiBIj2QMoNQI4N0PjEBgQECAxsigQABAQE 40, 37 -- X-IPAS-Result: A2GWAwDXnz5V/4Y1jApZA4MMU1wFxVSCNwqGAAICAoE1PBABAQEBAQEBA4EHgk86AQgEHQITKxICGQEBAQQBAQE3FB0aBAIBBQMRAwEBAQ0SCQcxARQDAQUIAgQKCQgOBwSICg3CW4RsAQEBAQEBAQMBAQEBAQEBARqLOIJ0AYEkCRACAQUJFxsSBQYLghxNgTMFhSyBFosTgy5ThjiBIj2QMoNQI4N0PjEBgQECAxsigQABAQE 40, 37 -- X-IronPort-AV: E=Sophos;i="5.11,659,1422946800"; 40, 37 -- d="scan'208";a="12582151" 40, 37 -- Received: from turquoise.catnet.arizona.edu (HELO smtp.catnet.arizona.edu) ([10.140.53.134]) 40, 37 -- by mails1n1out.email.arizona.edu with ESMTP/TLS/AES256-SHA; 27 Apr 2015 13:50:09 -0700 40, 37 -- Received: from CYAN.catnet.arizona.edu (10.140.53.35) by 40, 37 -- TURQUOISE.catnet.arizona.edu (10.140.53.134) with Microsoft SMTP Server (TLS) 40, 37 -- id 15.0.1076.9; Mon, 27 Apr 2015 13:50:09 -0700 40, 37 -- Received: from CYAN.catnet.arizona.edu ([fe80::1952:f9f4:e68b:e800]) by 40, 37 -- CYAN.catnet.arizona.edu ([fe80::1952:f9f4:e68b:e800%12]) with mapi id 40, 37 -- 15.00.1076.000; Mon, 27 Apr 2015 13:50:09 -0700 40, 37 -- From: "Cromey, Douglas W - (dcromey)" {dcromey-at-email.arizona.edu} 40, 37 -- To: "Microscopy listserv (Microscopy-at-Microscopy.Com)" 40, 37 -- {Microscopy-at-microscopy.com} 40, 37 -- Subject: RE: [Microscopy] Computer updates 40, 37 -- Thread-Topic: [Microscopy] Computer updates 40, 37 -- Thread-Index: AQHQgSck9DhEIgZ3y066LmnPQDBbS51hTGAQ 40, 37 -- Date: Mon, 27 Apr 2015 20:50:08 +0000 40, 37 -- Message-ID: {24aeb187f18d42b78c3ab6654c0cbeec-at-CYAN.catnet.arizona.edu} 40, 37 -- References: {201504272016.t3RKGTvi021133-at-ns.microscopy.com} 40, 37 -- In-Reply-To: {201504272016.t3RKGTvi021133-at-ns.microscopy.com} 40, 37 -- Accept-Language: en-US 40, 37 -- Content-Language: en-US 40, 37 -- X-MS-Has-Attach: 40, 37 -- X-MS-TNEF-Correlator: 40, 37 -- x-ms-exchange-transport-fromentityheader: Hosted 40, 37 -- x-originating-ip: [128.196.222.40] 40, 37 -- Content-Type: text/plain; charset="us-ascii" 40, 37 -- MIME-Version: 1.0 40, 37 -- Content-Transfer-Encoding: 8bit 40, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3RKoA0d008874 ==============================End of - Headers==============================
It's perfectly reasonable to tell the IT people you need XP or whatever to keep your instruments running. We're in the same boat with one of our confocals. A good approach to satisfy their need to upgrade and your need to not upgrade is to offer to let them pay for upgrading the hardware (computers) and software running your instruments. Or to not pay for the upgrades and leave you with your current systems on instruments.
Phil
} Dear all: } } I would like to revisit the problem of old software, computers, and institutional support. } } We have many instruments that run on proprietary software that has not been upgraded to more modern operating systems. } } For example, some of our instruments use programs only compatible with Windows XP. } } Our IT guys want to 'upgrade' all campus computers to a newer operating system and don't want any old machines running. } } Is it reasonable to tell them that we need our old XP (and earlier) computers to keep our instruments running. } } What would be a good approach to satisfy their urge to stay current and our need to live in the past? } } Thanks } } Jon } } Jonathan Krupp } Applied Science, Business& Technology } San Joaquin Delta College } 5151 Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } Find us on Facebook -at- } Electron Microscopy at SJ Delta College -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Mon Apr 27 15:54:52 2015 5, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3RKspKk014919 5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 15:54:51 -0500 5, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 5, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t3RKnwqi027737; 5, 32 -- Mon, 27 Apr 2015 16:54:45 -0400 5, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 5, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.195.1; Mon, 27 Apr 2015 16:47:55 -0400 5, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.195.1; Mon, 27 Apr 2015 16:47:53 -0400 5, 32 -- Message-ID: {553EA079.7070807-at-cmich.edu} 5, 32 -- Date: Mon, 27 Apr 2015 16:47:53 -0400 5, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: {jkrupp-at-deltacollege.edu} , micro {microscopy-at-microscopy.com} 5, 32 -- Subject: Re: [Microscopy] Computer updates 5, 32 -- References: {201504272029.t3RKTdgX005384-at-ns.microscopy.com} 5, 32 -- In-Reply-To: {201504272029.t3RKTdgX005384-at-ns.microscopy.com} 5, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit 5, 32 -- X-Originating-IP: [141.209.2.100] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -1.20 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,_L_SUPPORT,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 02Ol8SIN6 - f70364897d8f - 20150427 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
The volume involved in consumer manufacturing makes computer equipment cheap and expendable. That does not make it a reasonable proposition to scrap specialized equipment that lacks the economies of scale. Specialized instrumentation is neither cheap nor expendable. If the IT unit has difficulty understanding this, since you are at a college, you might consider getting an Econ faculty member involved who could assign a student project to evaluate the economics of the two alternatives: 1) isolate the security risks with a closed network running older operating systems; 2) scrap and replace with new instrumentation. Part of the cost of alternative #1 is that the interface boards from the scope manufacturer's may go out of production and repairs involving the interfaces may become more expensive for that reason. However, if the manufacturers know that numbers of their clients are addressing the software obsolescence problem with well-maintained older operating systems, pressure to hustle older systems off to the junkpile may diminish.
The rush to scrap and replace, solely for operating system compatibility issues, fails on all sorts of sustainability and economic grounds.
John Twilley
Consulting Scientist, New York
-----Original Message----- } From: jkrupp-at-deltacollege.edu } Sent: Apr 27, 2015 4:16 PM } To: jtwilley-at-sprynet.com } Subject: [Microscopy] Computer updates } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Yes a common pain from time to time. I'll just bullet point a couple of thoughts/discussion points (based on my lab's experiences)
1. dedicated control computers are part of an instrument. An upgrade of the computer part of the instrument implies IT needs to make sure that the device it attaches to still works ok - let them deal with the vendor (if a Win7, virus checker, security update friendly solution exists then great). The job of IT is, in part, to make sure you have a working system and that disruption is minimised. If the vendor tells them that their software won't work then it's time to discuss options. Anything you can do to massage the relationship with the IT people toward them realising they are upgrading a microscope rather than an office PC and the realistic costs of software induced hardware failure etc, the better things will go long term. Then you can get them to suggest solutions.
2. We solved the problem of "connection to the network" and virus transfer via USB a mixture of policy and tech solutions. For example. We have a TEM which runs Windows 2K on its control PC. The PC is connected via LAN to another PC running Windows 7 in a different room (this PC also connects the same way to some other microscopes). The PC also connects, via a separate LAN card, to the organisation LAN and the internet etc. It is fully patched and with security. To get files from the microscope users access the shared data drive on the microscope PC from the win 7 pc (which they can do while someone else is using the microscope). You then have a policy of no USB drive etc for the microscope PC which is considered to be "not on the network".
Hope there is something relevant in that. Best wishes, Duane
AgResearch Limited | Lincoln Research Centre | New Zealand Dr Duane P Harland, Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Tuesday, 28 April 2015 8:30 a.m. To: Harland, Duane
Dear all:
I would like to revisit the problem of old software, computers, and institutional support.
We have many instruments that run on proprietary software that has not been upgraded to more modern operating systems.
For example, some of our instruments use programs only compatible with Windows XP.
Our IT guys want to 'upgrade' all campus computers to a newer operating system and don't want any old machines running.
Is it reasonable to tell them that we need our old XP (and earlier) computers to keep our instruments running.
What would be a good approach to satisfy their urge to stay current and our need to live in the past?
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 20, 38 -- From prvs=155290bd1d=jkrupp-at-deltacollege.edu Mon Apr 27 15:15:58 2015 20, 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.175.111]) 20, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t3RKFvvC020511 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 15:15:58 -0500 20, 38 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 20, 38 -- by localhost (Email Security Appliance) with SMTP id 3D82F342714_53E98F9B 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 20:15:53 +0000 (GMT) 20, 38 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) 20, 38 -- by mailin.deltacollege.edu (Sophos Email Appliance) with SMTP id 330D72FD6F1_53E98F9F 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 20:15:53 +0000 (GMT) 20, 38 -- Received: from localhost (localhost [127.0.0.1]) 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C13C6FE5C545 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 20, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 20, 38 -- with ESMTP id IJghSG1cqNe5 for {Microscopy-at-microscopy.com} ; 20, 38 -- Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- Received: from localhost (localhost [127.0.0.1]) 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5DFC2FE5C547 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 20, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 20, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 20, 38 -- with ESMTP id 4rHh9uhMGHZx for {Microscopy-at-microscopy.com} ; 20, 38 -- Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- Received: from jonathans-mbp.deltacollege.edu (unknown [10.146.0.14]) 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 41E6FFE5C545 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) 20, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 20, 38 -- Content-Type: text/plain; charset=us-ascii 20, 38 -- Subject: Computer updates 20, 38 -- Date: Mon, 27 Apr 2015 13:15:53 -0700 20, 38 -- Message-Id: {B7150470-C9EA-44A4-B8DC-762A5F824F24-at-deltacollege.edu} 20, 38 -- To: Microscopy-at-microscopy.com 20, 38 -- Mime-Version: 1.0 (Apple Message framework v1085) 20, 38 -- X-Mailer: Apple Mail (2.1085) 20, 38 -- Content-Transfer-Encoding: 8bit 20, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3RKFvvC020511 ==============================End of - Headers==============================
Step 1: install Win.7/8 PC and disconnect XP/Win2K machine from network. Arguing with IT policy does not work but taking problem off their hands may.
Step 2: let old PC only run the equipment, do not use it for any other purposes.
Step 3: The old PC should have nothing except necessary app. program and hardware drivers. Transfer/remove all acquired data from it at the end of each session, this way there is nothing to backup. Just keep couple of spare installation disks with application and drivers.
Step 4: identify obsolete components i.e. motherboard with older bus type and proprietary interface card(s), etc. Buy spares while available. Or even the entire computer. Cheap for now but will become expensive or NLA just like components for old PDP-based EDS computers in the 90-s.
Data transfer can be done in many ways. Private network or USB drives or CDs or anything else. If XP machine becomes infected then system restore will likely fix it but if not then re-install OS and the application - easy with nothing to backup and no data loss. Besides a "lethal" malware is a rarity. My PC-related tech. support problems almost always about gigabytes of temp. files, junk applications plus disk cleanup and defrag not done in years.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 4/27/2015 4:17 PM, jkrupp-at-deltacollege.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all: } } I would like to revisit the problem of old software, computers, and institutional support. } } We have many instruments that run on proprietary software that has not been upgraded to more modern operating systems. } } For example, some of our instruments use programs only compatible with Windows XP. } } Our IT guys want to 'upgrade' all campus computers to a newer operating system and don't want any old machines running. } } Is it reasonable to tell them that we need our old XP (and earlier) computers to keep our instruments running. } } What would be a good approach to satisfy their urge to stay current and our need to live in the past? } } Thanks } } Jon } } Jonathan Krupp } Applied Science, Business & Technology } San Joaquin Delta College } 5151 Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } Find us on Facebook -at- } Electron Microscopy at SJ Delta College } } } } } } } } } } } ==============================Original Headers============================== } 20, 38 -- From prvs=155290bd1d=jkrupp-at-deltacollege.edu Mon Apr 27 15:15:58 2015 } 20, 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.175.111]) } 20, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t3RKFvvC020511 } 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 15:15:58 -0500 } 20, 38 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 20, 38 -- by localhost (Email Security Appliance) with SMTP id 3D82F342714_53E98F9B } 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 20:15:53 +0000 (GMT) } 20, 38 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) } 20, 38 -- by mailin.deltacollege.edu (Sophos Email Appliance) with SMTP id 330D72FD6F1_53E98F9F } 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 20:15:53 +0000 (GMT) } 20, 38 -- Received: from localhost (localhost [127.0.0.1]) } 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C13C6FE5C545 } 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) } 20, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 20, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) } 20, 38 -- with ESMTP id IJghSG1cqNe5 for {Microscopy-at-microscopy.com} ; } 20, 38 -- Mon, 27 Apr 2015 13:15:54 -0700 (PDT) } 20, 38 -- Received: from localhost (localhost [127.0.0.1]) } 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5DFC2FE5C547 } 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) } 20, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu } 20, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 20, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) } 20, 38 -- with ESMTP id 4rHh9uhMGHZx for {Microscopy-at-microscopy.com} ; } 20, 38 -- Mon, 27 Apr 2015 13:15:54 -0700 (PDT) } 20, 38 -- Received: from jonathans-mbp.deltacollege.edu (unknown [10.146.0.14]) } 20, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 41E6FFE5C545 } 20, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 13:15:54 -0700 (PDT) } 20, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 20, 38 -- Content-Type: text/plain; charset=us-ascii } 20, 38 -- Subject: Computer updates } 20, 38 -- Date: Mon, 27 Apr 2015 13:15:53 -0700 } 20, 38 -- Message-Id: {B7150470-C9EA-44A4-B8DC-762A5F824F24-at-deltacollege.edu} } 20, 38 -- To: Microscopy-at-microscopy.com } 20, 38 -- Mime-Version: 1.0 (Apple Message framework v1085) } 20, 38 -- X-Mailer: Apple Mail (2.1085) } 20, 38 -- Content-Transfer-Encoding: 8bit } 20, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3RKFvvC020511 } ==============================End of - Headers============================== }
Hi Jon, basically, all is said in the various answers. a) we - the admin's of the scopes - have to supervise not only the scopes and the (many) users but also all the IT associated with it, and we need to know quite well what's going on inside (if we have not done already); a good collaboration with the IT gurus is necessary ... or even try to turn into an IT guru ;-) b) } Our IT guys want to 'upgrade' all campus computers to a newer operating
} system and don't want any old machines running. --} this is understandable - but here, they already understood that this is not possible. They have accepted that "we need our old XP (and earlier) computers to keep our instruments running." c) } What would be a good approach to satisfy their urge to stay current and our } need to live in the past? here, all older machines (XP and older) are running in a local, secure network inside the campus, and not accessible from outside and with no access to the outside world. One major problem: all these 'drives' (e.g. USB etc) which become connected to the local machine for copying / storing images / data. One option is to stop any access to USB ports - and backing up all scientific data on a central storage area. Second problem: hardware issues on the old computers (solution: store old PC's for hardware backup). And, make people aware about 'our' problems. kind regards - Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: http://www.mc2015.de DGE - Microscopy Conference 2015, Sept 6-11, GĂśttingen http://www.emc2016.fr/en/ 16th Europ Microsc Congress EMC 2016 in Lyon, FR next Microbiol. conferences: VAAM - Annual Conf March 13-16, 2016, Jena
==============================Original Headers============================== 5, 24 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Apr 28 06:17:39 2015 5, 24 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3SBHcWG032645 5, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 06:17:38 -0500 5, 24 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 5, 24 -- by localhost (Postfix) with SMTP id DDDC64EAF9 5, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 13:17:34 +0200 (CEST) 5, 24 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 5, 24 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id A71274DB59 5, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 13:17:34 +0200 (CEST) 5, 24 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 5, 24 -- with Novell_GroupWise; Tue, 28 Apr 2015 13:17:34 +0200 5, 24 -- Message-Id: {553F886C0200005400053B77-at-gwsmtp1.uni-regensburg.de} 5, 24 -- X-Mailer: Novell GroupWise Internet Agent 14.0.1 5, 24 -- Date: Tue, 28 Apr 2015 13:17:32 +0200 5, 24 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 5, 24 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 5, 24 -- Subject: Computer updates 5, 24 -- References: {201504272055.t3RKt3u5015393-at-ns.microscopy.com} 5, 24 -- In-Reply-To: {201504272055.t3RKt3u5015393-at-ns.microscopy.com} 5, 24 -- Mime-Version: 1.0 5, 24 -- Content-Type: text/plain; charset=UTF-8 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- Content-Disposition: inline ==============================End of - Headers==============================
Hello Reinhard, We are facing the same problem with old computers and not supported operating systems. We use the same solution, a separate local network (hosting 1x Win3.11WfW â an old EDAX DX4 :-) , 1x Win98, 2x WinXP and 2x Win7). Here I would like to comment USB drives problem. After very bad experiences about 10 years ago, no user is allowed to copy data onto his/her own USB drive directly from microscope PC. For USB drive users we use following set-up. We have installed one linux box in our local network running Debian Wheezy and Samba server. Everyone who wants to copy data onto USB flash disk has to do it thru this linux box and Samba shared folders on the old Win systems.
With best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Tue, 28 Apr 2015 06:21:21 -0500, Reinhard.Rachel-at-biologie.uni-regensburg.de wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Jon, } basically, all is said in the various answers. } a) we - the admin's of the scopes - have to supervise not only the } scopes and the (many) users but also all the IT associated with it, } and we need to know quite well what's going on inside (if we have not } done already); a good collaboration with the IT gurus is } necessary ... or even try to turn into an IT guru ;-) } b) } Our IT guys want to 'upgrade' all campus computers to a newer } operating } } } system and don't want any old machines running. } --} this is understandable - but here, they already understood that } this is not possible. They have accepted that "we need our old XP } (and earlier) computers to keep our instruments running." } c) } What would be a good approach to satisfy their urge to stay } current and our } } need to live in the past? } here, all older machines (XP and older) are running in a local, } secure network inside the campus, and not accessible from outside and } with no access to the outside world. } One major problem: all these 'drives' (e.g. USB etc) which become } connected to the local machine for copying / storing images / data. } One option is to stop any access to USB ports - and backing up all } scientific data on a central storage area. Second problem: hardware } issues on the old computers (solution: store old PC's for hardware } backup). And, make people aware about 'our' problems. } kind regards - Reinhard } } -- } Prof. Dr. Reinhard Rachel } University of Regensburg } Centre for EM / Anatomy } Faculty of Biology & Preclin. Med. } Universitaetsstrasse 31 } D-93053 Regensburg - Germany } tel +49 941 943 -2837, -1720 } mail reinhard.rachel-at-biologie.uni-regensburg.de } office: VKL 3.1.29 } } Next microscopy conferences: } http://www.mc2015.de } DGE - Microscopy Conference 2015, Sept 6-11, GĂÂśttingen } http://www.emc2016.fr/en/ } 16th Europ Microsc Congress EMC 2016 in Lyon, FR } next Microbiol. conferences: } VAAM - Annual Conf March 13-16, 2016, Jena } } } } ==============================Original } Headers============================== 5, 24 -- From } Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Apr 28 06:17:39 2015 } 5, 24 -- Received: from rrzmta1.uni-regensburg.de } (rrzmta1.uni-regensburg.de [194.94.155.51]) 5, 24 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } t3SBHcWG032645 5, 24 -- for {Microscopy-at-microscopy.com} ; Tue, } 28 Apr 2015 06:17:38 -0500 5, 24 -- Received: from } rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 5, 24 -- by } localhost (Postfix) with SMTP id DDDC64EAF9 5, 24 -- for } {Microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 13:17:34 +0200 (CEST) } 5, 24 -- Received: from gwsmtp1.uni-regensburg.de } (gwsmtp1.uni-regensburg.de [132.199.5.51]) 5, 24 -- by } rrzmta1.uni-regensburg.de (Postfix) with ESMTP id A71274DB59 5, 24 -- } for {Microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 13:17:34 } +0200 (CEST) 5, 24 -- Received: from uni-regensburg-smtp1-MTA by } gwsmtp1.uni-regensburg.de 5, 24 -- with Novell_GroupWise; } Tue, 28 Apr 2015 13:17:34 +0200 5, 24 -- Message-Id: } {553F886C0200005400053B77-at-gwsmtp1.uni-regensburg.de} 5, 24 -- } X-Mailer: Novell GroupWise Internet Agent 14.0.1 5, 24 -- Date: Tue, } 28 Apr 2015 13:17:32 +0200 5, 24 -- From: "Reinhard Rachel" } {Reinhard.Rachel-at-biologie.uni-regensburg.de} 5, 24 -- To: "microscopy } listserver" {Microscopy-at-microscopy.com} 5, 24 -- Subject: Computer } updates 5, 24 -- References: } {201504272055.t3RKt3u5015393-at-ns.microscopy.com} 5, 24 -- In-Reply-To: } {201504272055.t3RKt3u5015393-at-ns.microscopy.com} 5, 24 -- } Mime-Version: 1.0 5, 24 -- Content-Type: text/plain; charset=UTF-8 5, } 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- Content-Disposition: } inline ==============================End of - } Headers==============================
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==============================Original Headers============================== 10, 41 -- From benada-at-biomed.cas.cz Tue Apr 28 08:17:38 2015 10, 41 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 10, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3SDHbeY021819 10, 41 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 08:17:38 -0500 10, 41 -- X-ASG-Debug-ID: 1430227056-05011e54d5886d0001-4CH8be 10, 41 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id JA9pVqlVkiBc36Bt (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 15:17:36 +0200 (CEST) 10, 41 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 10, 41 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 10, 41 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) 10, 41 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 10, 41 -- (No client certificate requested) 10, 41 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id E4AF92C80E9 10, 41 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 15:17:32 +0200 (CEST) 10, 41 -- Date: Tue, 28 Apr 2015 15:17:29 +0200 10, 41 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 41 -- To: {microscopy-at-microscopy.com} 10, 41 -- Subject: Fw: [Microscopy] Computer updates 10, 41 -- Message-ID: {20150428151729.209800bb-at-u117fs} 10, 41 -- X-ASG-Orig-Subj: Fw: [Microscopy] Computer updates 10, 41 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 41 -- =?UTF-8?B?xIxS?= 10, 41 -- X-Mailer: Claws Mail 3.10.1 (GTK+ 2.24.10; i486-pc-linux-gnu) 10, 41 -- MIME-Version: 1.0 10, 41 -- Content-Type: text/plain; charset=UTF-8 10, 41 -- X-IoP-CAS-MailScanner-ID: E4AF92C80E9.C05FF 10, 41 -- X-IoP-CAS-MailScanner: Processed 10, 41 -- X-Spam-Status: No 10, 41 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 10, 41 -- X-Barracuda-Start-Time: 1430227056 10, 41 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 10, 41 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 10, 41 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 10, 41 -- X-Barracuda-BRTS-Status: 1 10, 41 -- X-Barracuda-Spam-Score: 0.00 10, 41 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 10, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.18423 10, 41 -- Rule breakdown below 10, 41 -- pts rule name description 10, 41 -- ---- ---------------------- -------------------------------------------------- 10, 41 -- Content-Transfer-Encoding: 8bit 10, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3SDHbeY021819 ==============================End of - Headers==============================
Time: June 2 - 4th, 2015 Location: Yale Medical School, The Anlyan Center (TAC), New Haven, CT Cost: Free registration (on-line or on-site) Information: http://microscopy.med.yale.edu {http://microscopy.med.yale.edu/index.aspx}
Features: - Symposia on Lightsheet Microscopy ,electron tomography and Cryo-EM - Open access to state-of-the-art light and electron microscopes, as well as various sample preparation equipment (conventional and cryoEM) - Demonstrations, technical lectures, and walk-in clinics - Multiple vendor participation
There are two themes for the upcoming Symposium: "Lightsheet Microscopy" and "Visualizing sub-cellular structure in 3D by electron tomography and cryo-EM". We invite you to participate in a three day workshop. This free annual event is a combination of symposium and open access to a wide variety of fully functional microscopes. This year's symposium is focused on Lightsheet microscopy and Cryo-EM. We are very pleased to announce that several recent product releases will be available for hands-on use, including Lightsheet microscopes from Leica and LaVision BioTec and a Leica Cryo-EM system.
This event is designed for the busy researcher who wants to explore new techniques, see the latest in instrumentation, or simply get needed advice from vendor representatives. Reservations can be made for many of the instruments after registration. View this as an opportunity to perform a "surgical strike" exploration without any financial or time commitments. Feel free to come for advice from vendor representatives or to try out a microscope even if you are not attending a class or a seminar.
The confirmed speakers and schedule for electron tomography and cryo-EM are the following:
Tuesday, June 2nd, 9:005:00 PM
"Cryo-EM for molecular and cellular biology research" Wah Chiu, PhD National Center for Macromolecular Imaging, Baylor College of Medicine, Houston TX
"Sharpening our view of molecular machines that attach to filaments˛ Chuck Sindelar, PhD, Dept of Molecular Biophysics and Biochemistry Yale University
"Cryo-EM studies of microtubule-MAP interactions in vitro and in situ" Andreas Hoenger, PhD Dept of Molecular, Cellular and Developmental Biology, University of Colorado at Bolder
The workshop is open to the international research community and only requires free registration. Please see the website for free registration, a schedule of events, and list of equipment. http://microscopy.med.yale.edu {http://microscopy.med.yale.edu}
Please feel free to disseminate information about this workshop by word-of-mouth or through email at your institution. Need to try out an instrument that is not on the list but manufactured by a vendor that is already participating? Write to an organizer and we'll see if they can bring it.
We look forward to seeing you there - please reserve early.
The organizers, Ann Haberman ann.haberman-at-yale.edu {mailto:ann.haberman-at-yale.edu} Derek Toomre derek.toomre-at-yale.edu {mailto:derek.toomre-at-yale.edu} Joerg Bewersdorf joerg.bewersdorf-at-yale.edu {mailto:joerg.bewersdorf-at-yale.edu} Xinran Liu xinran.liu-at-yale.edu {mailto:xinran.liu-at-yale.edu}
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dwitters-at-caltech.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: dwitters-at-caltech.edu Name: Daan Witters
Organization: Caltech
Title-Subject: [Filtered] Critical point drying of hydrogel as sample prep for SEM
Message: Hi, I have a thin membrane that contains a hydrogel that has pores estimated to be 100-200 nm. I would like to visualize the pore structure on SEM and I heard that critical point drying is a suitable method for preparing my sample.
I wondered if my sample prep can be as easy as exchanging the water in my hydrogel with ethanol, placing the sample directly with ethanol in the critical point dryer, and dry the sample. If I want a cross-sectional view of the membrane, is it sufficient to cut the membrane while it is soaked in ethanol? Or are there other precautions I should take into account? Also, is there any recommendation for how much gold I can sputter on the dried sample? 50 nm of gold would already influence my pore size significantly and I don't know if gold gets deposited uniformly everywhere in the gel.
Thanks so much, Best, Daan Witters
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Others can probably comment better than I can about the entire plan, but there are a couple items I would address immediately.
First, I would be careful about cutting the membrane to reveal a cross section. I have clients cut samples to see the structure of a 300-nm layer on the surface. Even when using a fresh razor blade, they are surprised at the amount of damage left behind. It is ugly at 15kx. Your situation could be the same. However, if you are not looking at the surface but at a rather thick core areas, simple cutting might work for you.
We used to apply 15 nm of gold with our old coater. That is terribly thick by today's standards. We not often coat with as little as 2 nm of iridium. I would worry of you would still have charging due to the porous nature of your material. You probably want small volumes with something nearby to conduct away the charge. You might also want to look into variable pressure SEM to help mitigate charging.
Warren Straszheim
P.S. Now let me see how many vendors and labs that I have no interest in barrage me with out-of-office messages. Somehow sending out-of-office messages to someone you have no regular dealings with seems the antithesis of good customer service or public relations.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, April 28, 2015 3:59 PM To: Straszheim, Warren E [BIOTC]
X-from: dwitters-at-caltech.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dwitters-at-caltech.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: dwitters-at-caltech.edu Name: Daan Witters
Organization: Caltech
Title-Subject: [Filtered] Critical point drying of hydrogel as sample prep for SEM
Message: Hi, I have a thin membrane that contains a hydrogel that has pores estimated to be 100-200 nm. I would like to visualize the pore structure on SEM and I heard that critical point drying is a suitable method for preparing my sample.
I wondered if my sample prep can be as easy as exchanging the water in my hydrogel with ethanol, placing the sample directly with ethanol in the critical point dryer, and dry the sample. If I want a cross-sectional view of the membrane, is it sufficient to cut the membrane while it is soaked in ethanol? Or are there other precautions I should take into account? Also, is there any recommendation for how much gold I can sputter on the dried sample? 50 nm of gold would already influence my pore size significantly and I don't know if gold gets deposited uniformly everywhere in the gel.
Thanks so much, Best, Daan Witters
Login Host: 131.215.32.123 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 16, 45 -- From wesaia-at-iastate.edu Tue Apr 28 17:33:49 2015 16, 45 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1bn0100.outbound.protection.outlook.com [157.56.110.100]) 16, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3SMXnug031199 16, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 17:33:49 -0500 16, 45 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 16, 45 -- BLUPR04MB545.namprd04.prod.outlook.com (10.141.29.139) with Microsoft SMTP 16, 45 -- Server (TLS) id 15.1.148.16; Tue, 28 Apr 2015 22:33:48 +0000 16, 45 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.219]) by 16, 45 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.219]) with mapi id 16, 45 -- 15.01.0148.008; Tue, 28 Apr 2015 22:33:48 +0000 16, 45 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 16, 45 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 16, 45 -- CC: "dwitters-at-caltech.edu" {dwitters-at-caltech.edu} 16, 45 -- Subject: RE: [Microscopy] viaWWW:Critical point drying of hydrogel as sample 16, 45 -- prep for SEM 16, 45 -- Thread-Topic: [Microscopy] viaWWW:Critical point drying of hydrogel as sample 16, 45 -- prep for SEM 16, 45 -- Thread-Index: AQHQgfY5zYvc/Qo6sUWAxe5k7PJGm51i/+Zg 16, 45 -- Date: Tue, 28 Apr 2015 22:33:48 +0000 16, 45 -- Message-ID: {BLUPR04MB5469FE8809CACB1C85F2DB7D7E80-at-BLUPR04MB546.namprd04.prod.outlook.com} 16, 45 -- References: {201504282059.t3SKxOEE009453-at-ns.microscopy.com} 16, 45 -- In-Reply-To: {201504282059.t3SKxOEE009453-at-ns.microscopy.com} 16, 45 -- Accept-Language: en-US 16, 45 -- Content-Language: en-US 16, 45 -- X-MS-Has-Attach: 16, 45 -- X-MS-TNEF-Correlator: 16, 45 -- authentication-results: microscopy.com; dkim=none (message not signed) 16, 45 -- header.d=none; 16, 45 -- x-originating-ip: [129.186.227.4] 16, 45 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(42139001);SRVR:BLUPR04MB545; 16, 45 -- x-microsoft-antispam-prvs: {BLUPR04MB545DF7DADCE69EA66CDF628D7E80-at-BLUPR04MB545.namprd04.prod.outlook.com} 16, 45 -- x-forefront-antispam-report: BMV:1;SFV:NSPM;SFS:(10009020)(6009001)(252514010)(377454003)(13464003)(2351001)(50986999)(2656002)(89122001)(62966003)(99286002)(90282001)(106116001)(19580395003)(87936001)(76176999)(92566002)(76576001)(2501003)(46102003)(75432002)(122556002)(15395725005)(1720100001)(2900100001)(33656002)(2950100001)(86362001)(19580405001)(40100003)(54356999)(66066001)(74316001)(88552001)(110136001)(77156002)(15975445007)(102836002);DIR:OUT;SFP:1101;SCL:1;SRVR:BLUPR04MB545;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 16, 45 -- x-exchange-antispam-report-test: UriScan:; 16, 45 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5005006)(5002010)(3002001);SRVR:BLUPR04MB545;BCL:0;PCL:0;RULEID:;SRVR:BLUPR04MB545; 16, 45 -- x-forefront-prvs: 0560A2214D 16, 45 -- Content-Type: text/plain; charset="us-ascii" 16, 45 -- MIME-Version: 1.0 16, 45 -- X-OriginatorOrg: iastate.edu 16, 45 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 28 Apr 2015 22:33:48.7219 16, 45 -- (UTC) 16, 45 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 16, 45 -- X-MS-Exchange-CrossTenant-id: 0347d89a-0174-4dd3-adeb-3339c89c35f5 16, 45 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BLUPR04MB545 16, 45 -- Content-Transfer-Encoding: 8bit 16, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3SMXnug031199 ==============================End of - Headers==============================
Hi Daan, } I have a thin membrane that contains a hydrogel that has pores estimated } to be 100â200 nm. I would like to visualize the pore structure on SEM } and I heard that critical point drying is a suitable method for } preparing my sample. in principle, one might argue: "Yes, this may work"
} I wondered if my sample prep can be as easy as exchanging the water in } my hydrogel with ethanol, but, do you have any reason to believe that the pore size is the same (a) in water / aqueous environment, and (b) in the hydrogel in ethanol?
} placing the sample directly with ethanol in } the critical point dryer, and dry the sample. you might be able to do this, technically. But I assume that again, the pore size will be altered, by removing the ethanol, during CPD. (if you do not worry about this alteration: go ahead; you also may try air-drying - you might have done this already?)
} If I want a } crossâsectional view of the membrane, is it sufficient to cut the } membrane while it is soaked in ethanol? again, this is artificial .... you can do this, but how do you know what kind of alterations are introduced?
} Or are there other precautions I } should take into account? Also, is there any recommendation for how much } gold I can sputter on the dried sample? 50 nm of gold would already } influence my pore size significantly and I don't know if gold gets } deposited uniformly everywhere in the gel. if at all: platinum (or iridium) is the way to go, and as little as possible (something in the range of few nm; 2 to 3 nm is sufficient, for imaging in a modern SEM, at low kV, 1-2keV).
in fact, you might have to resort to cryo-SEM of uncoated, properly frozen samples, if you really want to get the real answer. Tricky, though, for the unexperienced ... (like me). You need to have the right cryo-SEM, and experience ... kind regards - Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: http://www.mc2015.de DGE - Microscopy Conference 2015, Sept 6-11, GĂśttingen http://www.emc2016.fr/en/ 16th Europ Microsc Congress EMC 2016 in Lyon, FR next Microbiol. conferences: VAAM - Annual Conf March 13-16, 2016, Jena
==============================Original Headers============================== 9, 24 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Apr 28 17:49:26 2015 9, 24 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3SMnPQq019232 9, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 17:49:26 -0500 9, 24 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 9, 24 -- by localhost (Postfix) with SMTP id 5330037980 9, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Apr 2015 00:49:25 +0200 (CEST) 9, 24 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 9, 24 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id 30ED6377A9 9, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Apr 2015 00:49:25 +0200 (CEST) 9, 24 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 9, 24 -- with Novell_GroupWise; Wed, 29 Apr 2015 00:49:25 +0200 9, 24 -- Message-Id: {55402A930200005400053BDD-at-gwsmtp1.uni-regensburg.de} 9, 24 -- X-Mailer: Novell GroupWise Internet Agent 14.0.1 9, 24 -- Date: Wed, 29 Apr 2015 00:49:23 +0200 9, 24 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 9, 24 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 9, 24 -- Subject: CPD of hydrogel - for SEM 9, 24 -- References: {201504282058.t3SKwSE6008940-at-ns.microscopy.com} 9, 24 -- In-Reply-To: {201504282058.t3SKwSE6008940-at-ns.microscopy.com} 9, 24 -- Mime-Version: 1.0 9, 24 -- Content-Type: text/plain; charset=UTF-8 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- Content-Disposition: inline ==============================End of - Headers==============================
CryoSEM is a good idea. With 200-300 nm pore size, you should be able to freeze the hydrogel onto a stub with little or no ice crystal formation within the membrane itself, then place on cryostage in the SEM and carefully etch away the ice. We've done this quite a bit with plant material, and plant cell walls are essentially hydrogels though with much smaller pores. You'd need to be able to do well-controlled coating in the cryotransfer unit, which the modern instruments should be capable of. Soaking the hydrogel in something conductive before freezing would help too and may allow you to image the membrane uncoated.
best regards, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1600 Canberra, ACT 2601 Australia T 61 2 6246 5475
________________________________________ X-from: Reinhard.Rachel-at-biologie.uni-regensburg.de [Reinhard.Rachel-at-biologie.uni-regensburg.de] Sent: Wednesday, 29 April 2015 8:54 a.m. To: White, Rosemary (Agriculture, Black Mountain)
Hi Daan, } I have a thin membrane that contains a hydrogel that has pores estimated } to be 100â200 nm. I would like to visualize the pore structure on SEM } and I heard that critical point drying is a suitable method for } preparing my sample. in principle, one might argue: "Yes, this may work"
} I wondered if my sample prep can be as easy as exchanging the water in } my hydrogel with ethanol, but, do you have any reason to believe that the pore size is the same (a) in water / aqueous environment, and (b) in the hydrogel in ethanol?
} placing the sample directly with ethanol in } the critical point dryer, and dry the sample. you might be able to do this, technically. But I assume that again, the pore size will be altered, by removing the ethanol, during CPD. (if you do not worry about this alteration: go ahead; you also may try air-drying - you might have done this already?)
} If I want a } crossâsectional view of the membrane, is it sufficient to cut the } membrane while it is soaked in ethanol? again, this is artificial .... you can do this, but how do you know what kind of alterations are introduced?
} Or are there other precautions I } should take into account? Also, is there any recommendation for how much } gold I can sputter on the dried sample? 50 nm of gold would already } influence my pore size significantly and I don't know if gold gets } deposited uniformly everywhere in the gel. if at all: platinum (or iridium) is the way to go, and as little as possible (something in the range of few nm; 2 to 3 nm is sufficient, for imaging in a modern SEM, at low kV, 1-2keV).
in fact, you might have to resort to cryo-SEM of uncoated, properly frozen samples, if you really want to get the real answer. Tricky, though, for the unexperienced ... (like me). You need to have the right cryo-SEM, and experience ... kind regards - Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: http://www.mc2015.de DGE - Microscopy Conference 2015, Sept 6-11, GĂśttingen http://www.emc2016.fr/en/ 16th Europ Microsc Congress EMC 2016 in Lyon, FR next Microbiol. conferences: VAAM - Annual Conf March 13-16, 2016, Jena
==============================Original Headers============================== 9, 24 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Apr 28 17:49:26 2015 9, 24 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3SMnPQq019232 9, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Apr 2015 17:49:26 -0500 9, 24 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 9, 24 -- by localhost (Postfix) with SMTP id 5330037980 9, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Apr 2015 00:49:25 +0200 (CEST) 9, 24 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 9, 24 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id 30ED6377A9 9, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Apr 2015 00:49:25 +0200 (CEST) 9, 24 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 9, 24 -- with Novell_GroupWise; Wed, 29 Apr 2015 00:49:25 +0200 9, 24 -- Message-Id: {55402A930200005400053BDD-at-gwsmtp1.uni-regensburg.de} 9, 24 -- X-Mailer: Novell GroupWise Internet Agent 14.0.1 9, 24 -- Date: Wed, 29 Apr 2015 00:49:23 +0200 9, 24 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 9, 24 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 9, 24 -- Subject: CPD of hydrogel - for SEM 9, 24 -- References: {201504282058.t3SKwSE6008940-at-ns.microscopy.com} 9, 24 -- In-Reply-To: {201504282058.t3SKwSE6008940-at-ns.microscopy.com} 9, 24 -- Mime-Version: 1.0 9, 24 -- Content-Type: text/plain; charset=UTF-8 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- Content-Disposition: inline ==============================End of - Headers==============================
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Email: gary-at-microtechnics.com Name: Dr. Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] TFE tips in Zeiss FESEMs
Message: Does anyone have any experiences, good or bad, with YPS versus Denka TFE 174 tips in Zeiss Gemini Supra SEMs? The cost difference is not huge but lifetime is a question as is performance. What about turning the gun off when not needed and back on for work?
Any input is greatly appreciated. TIA.
gary g.
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Message: I just had a big problem with the osmium fixative I used (2% in 0.1 M cacodylate buffer) turning a purplish black after 3 hours of fixation.
The tissue was slimy and basically ruined. Any ideas of what could have gone wrong? The osmium was perfectly clear and slightly yellow as always when I made the solution.
I also used the same type vials and buffer I have used before. What kind of solutions could cause this reaction?
Thank you
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The question really is: do you need an analysis of the structure of the pore or do you just need to determine their size accurately? If you "only" need the size, I would recommend other techniques than EM, because hydration is probably critical as it was already mentioned. Perhaps you could consider for example atomic force microscopy (AFM), which can be performed in liquid? Just my 2 cents.
Regards Stephane
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Subject: [Microscopy] viaWWW:Critical point drying of hydrogel as sample prep for SEM To: nizets2-at-yahoo.com Date: Tuesday, April 28, 2015, 11:05 PM
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Email: dwitters-at-caltech.edu Name: Daan Witters
Organization: Caltech
Title-Subject: [Filtered] Critical point drying of hydrogel as sample prep for SEM
Message: Hi, I have a thin membrane that contains a hydrogel that has pores estimated to be 100-200 nm. I would like to visualize the pore structure on SEM and I heard that critical point drying is a suitable method for preparing my sample.
I wondered if my sample prep can be as easy as exchanging the water in my hydrogel with ethanol, placing the sample directly with ethanol in the critical point dryer, and dry the sample. If I want a cross-sectional view of the membrane, is it sufficient to cut the membrane while it is soaked in ethanol? Or are there other precautions I should take into account? Also, is there any recommendation for how much gold I can sputter on the dried sample? 50 nm of gold would already influence my pore size significantly and I don't know if gold gets deposited uniformly everywhere in the gel.
Thanks so much, Best, Daan Witters
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Short and efficient: OOMDB (only over my dead body). Hope to hear from you soon! :-D
More seriously, I agree with others saying that you need to explain that these PCs are part of the instrument. You can't change them without changing the instrument.
Regards, Stephane
-------------------------------------------- On Mon, 4/27/15, jkrupp-at-deltacollege.edu {jkrupp-at-deltacollege.edu} wrote:
Subject: [Microscopy] Computer updates To: nizets2-at-yahoo.com Date: Monday, April 27, 2015, 10:21 PM
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Dear all:
I would like to revisit the problem of old software, computers, and institutional support.
We have many instruments that run on proprietary software that has not been upgraded to more modern operating systems.
For example, some of our instruments use programs only compatible with Windows XP.
Our IT guys want to 'upgrade' all campus computers to a newer operating system and don't want any old machines running.
Is it reasonable to tell them that we need our old XP (and earlier) computers to keep our instruments running.
What would be a good approach to satisfy their urge to stay current and our need to live in the past?
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
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The most common causes of Osmium turning color are because there is still glutaraldehyde in the sample or at the top of the tube which mixes with the osmium and oxidation occurs.
I buffer wash 3 times 20 minutes and make sure that I rinse the whole vial and inside of the cap then I wipe the top of the vial to dry it. The extended wash time is used to stop "peppering" of mitochondria and other dense structures. The osmium fixative is in cacodylate buffer, as you use, but at 1%. Several microscopists that I know do use 2% osmium. I have never gone much over an hour and do not understand the need for 3 hours as you mention. I had learned that osmium penetrates into a sample about 0.5mm in an hour then it starts to block itself from going deeper into a tissue sample. That is why we keep the size of all samples to 1mm cubic or smaller. If a larger sample is cut in two after an hour one can see that it is white in the center and hence the osmium did not reach that point to do its work as a fixative.
I had a sample back in 2007 or 2008, that had a huge amount of fat in it. I saw that the osmium did start to turn within the hour. I assume that the glutaraldehyde was not washed out completely, for back then I buffer washed only a half hour.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Pat
Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491 connellyps-at-mail.nih.gov
On 4/29/15 1:26 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
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We are inviting applications for a Postdoctoral Researcher in the laboratory of Prof. Marek Basler at the Biozentrum of University of Basel (full time position).
======================================================== Structure of the bacterial Type VI secretion system by cryo-EM ========================================================
The Type VI secretion system (T6SS) is a unique protein-translocating bacterial nanomachine that is fascinating from several points of view: (i) T6SS clusters are present in numerous pathogenic bacteria, (ii) T6SS can deliver effectors into both bacterial and eukaryotic targets, (iii) components of T6SS are evolutionarily related to contractile phage tails, (iv) dynamics of T6SS can be studied using fluorescence microscopy in live cells allowing unprecedented insights into function of this complex nanomachine.
More info: http://www.biozentrum.unibas.ch/basler/ Basler, M., et al., Nature 2012, 483, 182â186. Kudryashev, M., et al., Cell 2015, 160, 952â962.
In this project, we aim to obtain the high-resolution structure of the whole T6SS assembly in various model organisms using cryo-electron microscopy. The goal is to obtain mechanistic insight into T6SS function and explain differences in T6SS dynamics between model organisms. We will primarily focus on the following biological questions: How is T6SS tail attached to the cell wall? What are differences between pre- and post- contraction structures of T6SS? What are the fundamental structural differences between model organisms?
We collaborate with Prof. Henning Stahlberg and have access to C-CINA (http://www.c-cina.unibas.ch/) equipped with a FEI Titan Krios with Quantum-LS GIF / K2-Summit and a FEI Polara with K2-Summit. Extensive computing infrastructure is available. Starting date by arrangement (approx. second half of 2015). Long term funding is secured from an SNSF grant. Salary according to SNSF guidelines.
Qualifications:
Candidates holding a PhD diploma with experience in cryo-electron microscopy and/or cryo-electron tomography of bacteria and image processing of such data are encouraged to apply. The working language is English.
How to apply:
Please, send your CV (max 3 pages), a brief description of previous research experience (1 page), a motivation letter describing your interests in our lab (1 page), and the names of two references to Dr. Anne-CĂŠcile Hiebel: basler-recruitment-at-unibas.ch. Deadline: Jul-31-2015.
The Biozentrum of the University of Basel is one of the leading institutes worldwide for molecular and biomedical basic research and teaching. It is home to more than 30 research groups with scientists from over 40 countries. Research at the Biozentrum focuses on the areas of Cell Growth & Development, Infection Biology, Neurobiology, Structural Biology & Biophysics and Computational & Systems Biology. With its more than 550 employees, the Biozentrum is the largest department at the University of Baselâs Faculty of Science.
Marek Basler, Biozentrum, University Basel
==============================Original Headers============================== 15, 38 -- From henning.stahlberg-at-unibas.ch Wed Apr 29 11:08:10 2015 15, 38 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 15, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3TG8AhB009029 15, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Apr 2015 11:08:10 -0500 15, 38 -- Received: from localhost (localhost [127.0.0.1]) 15, 38 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 17A0D220144 15, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Apr 2015 18:08:09 +0200 (CEST) 15, 38 -- X-Virus-Scanned: amavisd-new at unibas.ch 15, 38 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 15, 38 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 15, 38 -- with LMTP id ijC8T1PnV5IR for {Microscopy-at-microscopy.com} ; 15, 38 -- Wed, 29 Apr 2015 18:08:09 +0200 (CEST) 15, 38 -- Received: from URZ-HT-CAS-3.urz.unibas.ch (urz-ht-cas-3.urz.unibas.ch [131.152.8.133]) 15, 38 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 054AB22011C 15, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Apr 2015 18:08:09 +0200 (CEST) 15, 38 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 15, 38 -- URZ-HT-CAS-3.urz.unibas.ch ([fe80::cc17:655b:f0b:e110%11]) with mapi id 15, 38 -- 14.03.0224.002; Wed, 29 Apr 2015 18:08:08 +0200 15, 38 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 15, 38 -- To: Microscopy {Microscopy-at-microscopy.com} 15, 38 -- CC: Marek Basler {marek.basler-at-unibas.ch} 15, 38 -- Subject: PostDoc in Cryo-Electron Tomography of T6SS in the Basler lab at 15, 38 -- the University of Basel, Switzerland, available 15, 38 -- Thread-Topic: PostDoc in Cryo-Electron Tomography of T6SS in the Basler lab 15, 38 -- at the University of Basel, Switzerland, available 15, 38 -- Thread-Index: AQHQgpauOg9gNyrYMUeMYbMqb+EimA== 15, 38 -- Date: Wed, 29 Apr 2015 16:08:07 +0000 15, 38 -- Message-ID: {643CEA1E-171C-4944-AD66-ACC09074C4F7-at-unibas.ch} 15, 38 -- Accept-Language: en-US, de-CH 15, 38 -- Content-Language: en-US 15, 38 -- X-MS-Has-Attach: 15, 38 -- X-MS-TNEF-Correlator: 15, 38 -- x-originating-ip: [131.152.8.190] 15, 38 -- Content-Type: text/plain; charset="utf-8" 15, 38 -- Content-ID: {10A6FCD3E9C1B645994A54DCB6A257F8-at-zuv.ads.unibas.ch} 15, 38 -- MIME-Version: 1.0 15, 38 -- Content-Transfer-Encoding: 8bit 15, 38 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t3TG8AhB009029 ==============================End of - Headers==============================
We have a user who wants to do mapping with non-square pixels. That is, the step size in X would be different than the step size in Y. I cannot see any way to do this in DM. Does anyone have any suggestions?
Thanks.
John Mardinly, ASU
==============================Original Headers============================== 4, 35 -- From John.Mardinly-at-asu.edu Thu Apr 30 14:57:24 2015 4, 35 -- Received: from bcnetw1.asu.edu (bcnetw1.asu.edu [149.169.2.71]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3UJvNLn013560 4, 35 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2015 14:57:24 -0500 4, 35 -- X-ASG-Debug-ID: 1430423840-0671b54ca801b70005-4CH8be 4, 35 -- Received: from exhubw01.asurite.ad.asu.edu (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnetw1.asu.edu with ESMTP id EkucLl9zoBjP74Wa (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2015 12:57:22 -0700 (MST) 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 4, 35 -- X-ASG-Whitelist: Client 4, 35 -- Received: from EXMBW02.asurite.ad.asu.edu ([169.254.9.128]) by 4, 35 -- exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id 14.03.0210.002; 4, 35 -- Thu, 30 Apr 2015 12:54:39 -0700 4, 35 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 35 -- Subject: Gatan Digital Microscopy Question 4, 35 -- Thread-Topic: Gatan Digital Microscopy Question 4, 35 -- X-ASG-Orig-Subj: Gatan Digital Microscopy Question 4, 35 -- Thread-Index: AdCDf309BaoJLVW+S5mfsbma9gR/pg== 4, 35 -- Date: Thu, 30 Apr 2015 19:54:37 +0000 4, 35 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9401C6E64F7-at-exmbw02.asurite.ad.asu.edu} 4, 35 -- Accept-Language: en-US 4, 35 -- Content-Language: en-US 4, 35 -- X-MS-Has-Attach: 4, 35 -- X-MS-TNEF-Correlator: 4, 35 -- x-originating-ip: [129.219.4.240] 4, 35 -- Content-Type: text/plain; charset="us-ascii" 4, 35 -- MIME-Version: 1.0 4, 35 -- X-Barracuda-Connect: exhubw01.asurite.ad.asu.edu[129.219.4.199] 4, 35 -- X-Barracuda-Start-Time: 1430423842 4, 35 -- X-Barracuda-Encrypted: AES128-SHA 4, 35 -- X-Barracuda-URL: https://149.169.2.71:443/cgi-mod/mark.cgi 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 35 -- X-Barracuda-BRTS-Status: 1 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3UJvNLn013560 ==============================End of - Headers==============================
From no-reply-at-financeservicesltd.com Sun May 3 18:10:58 2015 Return-Path: {no-reply-at-financeservicesltd.com} Received: from doori11.dooricare.com ([211.218.144.13]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t43NArD9023699; Sun, 3 May 2015 18:10:57 -0500 Received: from User (217-19-105-202.synterra-ug.ru [217.19.105.202] (may be forged)) (authenticated bits=0) by doori11.dooricare.com (8.14.1/8.14.1) with ESMTP id t43N6fWE009771; Mon, 4 May 2015 08:06:47 +0900 Message-Id: {201505032306.t43N6fWE009771-at-doori11.dooricare.com}
Hi Listers,
I have a user who needs a cryo-SEM (and we do not have one). Could you please direct me to closest to Kansas City area facility where he can have work done (for a fee).
Thanks,
Vladimir
Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Director
(816) 235-2072
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784
==============================Original Headers============================== 13, 28 -- From DusevichV-at-umkc.edu Tue May 5 09:10:01 2015 13, 28 -- Received: from um-nip4-umkc-out.um.umsystem.edu (um-nip4-umkc-out.um.umsystem.edu [198.209.49.180]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t45EA1xv007657 13, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 5 May 2015 09:10:01 -0500 13, 28 -- X-IronPort-Anti-Spam-Filtered: true 13, 28 -- X-IronPort-Anti-Spam-Result: A2EXBQCSzkhV/8SeoM9ZA4MMgTTLAYJeAoE1PBABAQEBAQEBA4EHhCIFOlEBKhRCJgEEG4gkoVyeXwGEVgEKAQEBAR2QDSEogwaBFgWSHYt6g1aRNSNggxSCM4EBAQEB 13, 28 -- X-IPAS-Result: A2EXBQCSzkhV/8SeoM9ZA4MMgTTLAYJeAoE1PBABAQEBAQEBA4EHhCIFOlEBKhRCJgEEG4gkoVyeXwGEVgEKAQEBAR2QDSEogwaBFgWSHYt6g1aRNSNggxSCM4EBAQEB 13, 28 -- Received: from um-tcas1.um.umsystem.edu ([207.160.158.196]) 13, 28 -- by um-nip4-exch-relay.um.umsystem.edu with ESMTP; 05 May 2015 09:10:01 -0500 13, 28 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.165]) by 13, 28 -- UM-TCAS1.um.umsystem.edu ([207.160.158.196]) with mapi id 14.03.0235.001; 13, 28 -- Tue, 5 May 2015 09:10:00 -0500 13, 28 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 13, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 13, 28 -- Subject: Cryo-SEM in Kansas City area 13, 28 -- Thread-Topic: Cryo-SEM in Kansas City area 13, 28 -- Thread-Index: AdCHPPzvSqxSTx3xRbCssILccCnFRw== 13, 28 -- Date: Tue, 5 May 2015 14:10:00 +0000 13, 28 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DC5D512-at-UM-MBX-T01.um.umsystem.edu} 13, 28 -- Accept-Language: en-US 13, 28 -- Content-Language: en-US 13, 28 -- X-MS-Has-Attach: 13, 28 -- X-MS-TNEF-Correlator: 13, 28 -- x-originating-ip: [134.193.156.189] 13, 28 -- Content-Type: text/plain; charset="us-ascii" 13, 28 -- MIME-Version: 1.0 13, 28 -- Content-Transfer-Encoding: 8bit 13, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t45EA1xv007657 ==============================End of - Headers==============================
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Email: marie.cantino-at-uconn.edu Name: Marie Cantino
Organization: University of Connecticut
Title-Subject: [Filtered] Staff Position, Electron Microscopy Specialist
Message: The Department of Physiology and Neurobiology at the University of Connecticut seeks applications for an Electron Microscopy Specialist (Academic Assistant 1 or 2). This is a full-time, annually renewable position. The successful candidate will work in the Bioscience Electron Microscopy Laboratory (BEML) located on the main campus in Storrs. The successful candidate will be in charge of transmission electron microscopy (TEM) services, assisting scientists in experimental design and interpretation of results, preparation of biological and organic samples and TEM operation, as well as training and supervision of students in these procedures. Other duties may include assisting other laboratory staff in carrying out projects and training associated with scanning electron microscopy, and routine laboratory maintenance. Go to www.jobs.uconn.edu, Staff Openings, Job ID# 2015312, for more information.
Screening of applications will begin immediately and will continue until the position is filled.
The University of Connecticut is an EEO/AA employer.
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ. of Idaho
Title-Subject: [Filtered] 2015 "Lunch with a Manager" -at- MM Annual Meeting in Portland
Message: Greetings,
The Facilities Operations & Management FIG is pleased to once again offer our Lunch w/Manager round-table. We are also pleased to have financial support from JEOL Ltd. If you would be so kind as to distribute the information below to your students, post-docs, colleagues, or anyone you believe might be interested.
I would be most appreciative! See you in Portland. Tom Williams
An Invitation to the Membership:
2015 Microscopy & Microanalysis Annual Meeting Portland, OR ÂLunch with a Manager Hosted by Facilities Operations & Management Focused Interest Group & Sponsored by JEOL Ltd.
The MSA FOM-FIG is pleased to host a Lunch & Roundtable forum with a panel of veteran Facility Managers.
Discussion Topics: --ÂIÂm from Venus, the Dean is from Mars!Â: Communication with Administration --Open Questions round-table
The lunches are limited to the first 20 registered participants! Walk-ins are welcome depending on available seating.
Deadline to register: 28 July
To Register: Contact Tom Williams at the University of Idaho: tomw-at-uidaho.edu -provide a contact email, institutional affiliation, and any dietary accommodations or restrictions.
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==============================Original Headers============================== 26, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue May 5 18:41:58 2015 26, 28 -- Received: from znl.com ([206.69.208.20]) 26, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t45NfwhZ011922 26, 28 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2015 18:41:58 -0500 26, 28 -- Received: from localhost (localhost [127.0.0.1]) 26, 28 -- by znl.com (Postfix) with ESMTP id 53F1A44981C 26, 28 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2015 18:41:58 -0500 (CDT) 26, 28 -- X-Virus-Scanned: amavisd-new at znl.com 26, 28 -- Received: from znl.com ([127.0.0.1]) 26, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 26, 28 -- with ESMTP id Jpn2FNWfGen1 for {microscopy-at-microscopy.com} ; 26, 28 -- Tue, 5 May 2015 18:41:49 -0500 (CDT) 26, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 26, 28 -- by znl.com (Postfix) with ESMTPA id E0FD0449810 26, 28 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2015 18:41:49 -0500 (CDT) 26, 28 -- Message-ID: {5549553D.60808-at-microscopy.com} 26, 28 -- Date: Tue, 05 May 2015 18:41:49 -0500 26, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 26, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 26, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 26, 28 -- MIME-Version: 1.0 26, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 26, 28 -- Subject: viaWWW:2015 "Lunch with a Manager" -at- MM Annual Meeting in Portland 26, 28 -- References: {201505051640.t45GeGHt031571-at-ns.microscopy.com} 26, 28 -- In-Reply-To: {201505051640.t45GeGHt031571-at-ns.microscopy.com} 26, 28 -- X-Forwarded-Message-Id: {201505051640.t45GeGHt031571-at-ns.microscopy.com} 26, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 26, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: kristian.molhave-at-nanotech.dtu.dk Name: Kristian Mølhave
Organization: DTU Nanotech
Title-Subject: [Filtered] Postdoc position in Developing Electron Microscopy of Electrochemical Processes in Liquids
Message: Postdoc position in Developing Electron Microscopy of Electrochemical Processes in Liquids
Application deadline 17th may 2015
Description and online application form: http://www.nanotech.dtu.dk/english/About-NTCH/Jobs/job?id=60b546be-0212-48a3-8c40-c26f26d20f8a
DTU Nanotech at the Technical University of Denmark invites applications for a position as postdoc in Developing Electron Microscopy of Electrochemical Processes in Liquids.
Electron microscopic imaging of physical and chemical processes in liquids with atomic scale resolution has become possible in the last couple of years by using special microchip based systems that can contain the liquid sample behind ultra-thin electron transparent membranes. The aim of this project is to develop new nanoscale imaging systems based on microfluidic chips, including electrically contacted electrodes in the chip systems, to enable electron microscopy of electrochemical processes in liquids.
In this project you will be developing such microfabricated devices that will make it possible to observe a range of electrochemical processes in new ways using both scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
The project will involve development and microfabrication of chip systems in our 1300 m2 state of art clean room facility Danchip; imaging in our electron microscopy facility CEN, using SEM and TEM to observe and characterize fundamental electrochemical processes. The work will be in close collaboration with microscopists, electrochemists and industrial partners in the project.
Responsibilities and tasks The Post Doc project will involve:  Electron microscopy at DTU advanced microscopy facility CEN  The development of microchip systems in our cleanroom  Performing experiments and detailed analysis of the chemical and physical processes in collaboration with the project partners.  Participate in supervision of students and close collaboration with PhD students in the group.
Qualifications Candidates should have a PhD degree in engineering or a similar degree with an academic level equivalent to the PhD degree in engineering. A background in chemistry and/or physics and experience with one or more of the involved methods will be beneficial, such as:  TEM methods such as STEM, EELS, EDX and holography  Electrochemical measurements  Cleanroom fabrication
Assessment The assessment of the applicants will be made by Kristian Mølhave, Head of the Molecular Windows group at DTU Nanotech, Jakob Wagner Professor at the Center for Electron Nanoscopy and Ole Hansen, Professor at DTU Nanotech.
We offer We offer an interesting and challenging job in an international environment focusing on education, research, public-sector consultancy and innovation, which contribute to enhancing the economy and improving social welfare. We strive for academic excellence, collegial respect and freedom tempered by responsibility. The Technical University of Denmark (DTU) is a leading technical university in northern Europe and benchmarks with the best universities in the world.
Salary and terms of employment The appointment will be based on the collective agreement with the Confederation of Professional Associations. The allowance will be agreed with the relevant union. The employment until 28 February 2018, starting as soon as possible.
Further information Further information may be obtained from Kristian Mølhave, Head of the Molecular Windows group at DTU Nanotech on Kristian.molhave-at-nanotech.dtu.dk or tel.: +45 2512 6672.
The project is financed by the FTP Sapere Aude project LiquidEM.
You can read more about the Molecular Windows group on www.nanotech.dtu.dk/mowin.
Application procedure: Please submit your online application no later than 17th May 2015. Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply online," fill in the online application form, and attach all your materials in English in one PDF file. The file must include: Â Application (cover letter) Â CV Â Diploma (an official translation into English) Â List of publications Applications and enclosures received after the deadline will not be considered.
All interested candidates irrespective of age, gender, disability, race, religion or ethnic background are encouraged to apply.
DTU Nanotech - the Department of Micro- and Nanotechnology is a centre of excellence in micro- and nanotechnology exploiting sciences across the traditional boundaries of technology, thereby enabling innovative solutions for the benefit of society. DTU Nanotech has approx. 220 people on its staff with 40 % international employees creating a highly international working environment.
The Center for Electron Nanoscopy (CEN) has a suite of seven complementary electron microscopes, comprising two scanning electron microscopes (SEMs), two dual beam (SEM combined with a focused ion beam, FIB), an FEI Tecnai transmission electron microscope (TEM) and two Âhigh end FEI Titan TEMs.
DTU Danchip is the national Danish center for micro- and nanofabrication, providing state-of-the-art processing equipment within the 1300 m2 cleanroom facilities.
DTU is a technical university providing internationally leading research, education, innovation and public service. Our staff of 5,700 advance science and technology to create innovative solutions that meet the demands of society; and our 10,000 students are being educated to address the technological challenges of the future. DTU is an independent academic university collaborating globally with business, industry, government, and public agencies.
Group homepage http://www.nanotech.dtu.dk/mowin
With best regards Kristian ___________________________ Kristian Mølhave Associate Professor / Lektor, Ph.D. Molecular Windows group leader www.nanotech.dtu.dk/mowin DTU Nanotech - Dept. of Micro and Nanotechnology 345e-252 Technical University of Denmark Mobile Phone (0045) 2512 6672 E-mail: kristian.molhave-at-nanotech.dtu.dk http://kristian.molhave.dk
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Email: Linda.Davis-at-Vesuvius.com Name: Linda Davis
Message: Hello All, We are getting rid of an old Struers Rotopol-31 grinding polishing machine with Rotoforce 4 and Multidoser (with Rotocom). We had a custom-machined head made for the Rotoforce, which holds 4 thin-section samples. We have numerous discs and cloths and supplies to go with this. If you want this, itÂs yours for either pickup or the shipping costs. Please contact me at Linda.Davis-at-Vesuvius.com if you are interested.
Linda Davis
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Hi All This year at M&M in Portland the ProjectMICRO workshop Tuesday afternoon, will be hosted by The Private Eye . There's a great portrait of The Private Eye in the current issue of Microscopy Today. Click the link, type in page 52 at the top of the page. Or scroll to page 52.
Perhaps you could pass along to like-minded administrators, or educators who might be attending the M&M Conference?
Best wishes Elaine Humphrey
Dr. Elaine C. Humphrey Advanced Microscopy Facility University of Victoria, Canada Lab: 250-853-3968 cell: 250-886-2068 website: http://www.stehm.uvic.ca
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Email: lstafford-at-sono-tek.com Name: Lisa
Organization: Sono-Tek
Title-Subject: [Filtered] Proximity of Sputter Coater to SEM
Message: Dear Colleagues,
I'm in the process of reorganizing my lab and I currently have my sputter coater and JEOL JCM-6000 Benchtop SEM placed 115 cm apart. My concern is that if the column is open to the atmosphere and the sputter coater is run that the column may get contaminated.
Is this a legitimate concern?
Thank you for your time,
Lisa
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed May 6 16:49:37 2015 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t46LnbAY001560 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2015 16:49:37 -0500 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id D804144CAD3 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2015 16:49:37 -0500 (CDT) 15, 28 -- X-Virus-Scanned: amavisd-new at znl.com 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id 1V08AIc-SIM8 for {microscopy-at-microscopy.com} ; 15, 28 -- Wed, 6 May 2015 16:49:30 -0500 (CDT) 15, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 8513844CAC6 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2015 16:49:30 -0500 (CDT) 15, 28 -- Message-ID: {554A8C6A.8060707-at-microscopy.com} 15, 28 -- Date: Wed, 06 May 2015 16:49:30 -0500 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:Proximity of Sputter Coater to SEM 15, 28 -- References: {201505061521.t46FLbuh000464-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201505061521.t46FLbuh000464-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201505061521.t46FLbuh000464-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
With all due respect I think you have your priorities wrong!
A sputter coater, like any other piece of equipment using a rotary pump, can be a contamination device due to the pump fluid vapour which is brought out of the pump with the gas. This contamination will pollute the laboratory air, fall on surfaces and on people. But even these problems should not worry you as much as your staff breathing this contaminated air!
Please make sure you use the best possible filter on the pump, and change it regularly; to wait until you smell the contamination, in my mind, too late! Alternatively, vent the pump to the outside world. Have in mind that the way to clean out some of the contamination within any rotary pump fluid, is to run a high level of air/gas through it. As the sputter coater rotary pump starts to operate it ejects a high level of gas which will contain pump fluid. Even under its actual operating conditions you are purging gas into the system that is also passing through the pump fluid. Add to this that the sputter coater is usually the first instrument to put the specimens under vacuum, thus it has all the vapours from the specimen to cope with. This combination of vapours, and chemical reactions within the pump fluid, result in the smell you sense if the filter fitted to the pump is unable to cope.
I do hope that this helps you and the many others who should be aware of rotary pump vapours?
Regards
Steve
Steve Chapman FRMS Electron Microscopy Consultancy and Training www.emcourses.com Cell +44 (0)7711606967
Title-Subject: [Filtered] Proximity of Sputter Coater to SEM
Message: Dear Colleagues,
I'm in the process of reorganizing my lab and I currently have my sputter coater and JEOL JCM-6000 Benchtop SEM placed 115 cm apart. My concern is that if the column is open to the atmosphere and the sputter coater is run that the column may get contaminated.
Is this a legitimate concern?
Thank you for your time,
Lisa
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==============================Original Headers============================== 24, 22 -- From protrain-at-emcourses.com Thu May 7 03:42:32 2015 24, 22 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 24, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t478gWus027795 24, 22 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2015 03:42:32 -0500 24, 22 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 24, 22 -- id {B554b25650000} ; Thu, 07 May 2015 09:42:13 +0100 24, 22 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Thu, 7 May 2015 09:42:12 +0100 24, 22 -- From: "Steve Chapman" {protrain-at-emcourses.com} 24, 22 -- To: {lstafford-at-sono-tek.com} 24, 22 -- Cc: {microscopy-at-microscopy.com} 24, 22 -- References: {201505062151.t46Lpg1E002637-at-ns.microscopy.com} 24, 22 -- In-Reply-To: {201505062151.t46Lpg1E002637-at-ns.microscopy.com} 24, 22 -- Subject: RE: [Microscopy] viaWWW:Proximity of Sputter Coater to SEM 24, 22 -- Date: Thu, 7 May 2015 09:42:20 +0100 24, 22 -- Message-ID: {002101d088a1$bb1d34b0$31579e10$-at-com} 24, 22 -- MIME-Version: 1.0 24, 22 -- Content-Type: text/plain; 24, 22 -- charset="us-ascii" 24, 22 -- Content-Transfer-Encoding: 7bit 24, 22 -- X-Mailer: Microsoft Office Outlook 12.0 24, 22 -- Thread-Index: AdCIRs/ZM/eBKgjAR2umhtaL1nPJhQAVsajg 24, 22 -- Content-Language: en-gb ==============================End of - Headers==============================
This is not a notification regarding employment position application. My retirement is approaching and we are considering various options for continuing electron and light microscopy core resources at UCSF. Please respond to me if you have an interest in a 40â60% position managing and operating our transmission and scanning electron microscopy core located on the San Francisco Parnassus Avenue campus. The duties will include maintaining the instruments, training new users, developing new services, preparing samples including thin sectioning or teaching users how to prepare samples ranging from negative staining, immuno-labeling, cell cultures and tissues of many types including dental non-organic samples, operation of the microscopes for or with users, administrative duties including preparation of recharges and marketing. It is helpful to have experience with a multi-user facility, knowledge of SerialEM software, confocal, widefield fluorescence and other light microscopy instrumentation and techniques.
-- Larry Ackerman, Specialist Electron Microscopy Lab Manager, Diabetes & Endocrinology Research Center Microscopy Core UCSF, Dept. of Anatomy, Rm S657 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-999-4758
==============================Original Headers============================== 7, 58 -- From btv1==56964010fc3==Larry.Ackerman-at-ucsf.edu Thu May 7 14:18:52 2015 7, 58 -- Received: from esa2.ucsf.iphmx.com (esa2.ucsf.iphmx.com [68.232.143.34]) 7, 58 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t47JIp1H003954 7, 58 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2015 14:18:51 -0500 7, 58 -- Received: from mcbmobwap001.ucsfmedicalcenter.org ([64.54.35.18]) 7, 58 -- by esa2.ucsf.iphmx.com with ESMTP/TLS/DHE-RSA-AES256-SHA; 07 May 2015 12:18:50 -0700 7, 58 -- X-AuditID: 40362312-f79716d0000032b8-e4-554bba9a0e0b 7, 58 -- Received: from bcuda5.ucsf.edu (otp005580ots.ucsfmedicalcenter.org [64.54.36.202]) 7, 58 -- by mcbmobwap001.ucsfmedicalcenter.org (Symantec Mail Security) with SMTP id C3.19.12984.A9ABB455; Thu, 7 May 2015 12:18:50 -0700 (PDT) 7, 58 -- X-ASG-Debug-ID: 1431026032-0547732c237bae90006-1DjkGe 7, 58 -- Received: from exht05.net.ucsf.edu (mx.ucsf.edu [64.54.247.193]) by bcuda5.ucsf.edu with ESMTP id Gv3jcMgzbvTflEJU (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-microscopy.com} ; Thu, 07 May 2015 12:13:54 -0700 (PDT) 7, 58 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu 7, 58 -- X-Barracuda-Apparent-Source-IP: 64.54.247.193 7, 58 -- Received: from [192.168.0.101] (128.218.141.134) by exht05.net.ucsf.edu 7, 58 -- (64.54.247.222) with Microsoft SMTP Server id 14.3.224.2; Thu, 7 May 2015 7, 58 -- 12:13:51 -0700 7, 58 -- Message-ID: {554BB96F.8070206-at-ucsf.edu} 7, 58 -- Date: Thu, 7 May 2015 12:13:51 -0700 7, 58 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu} 7, 58 -- Reply-To: {larry.ackerman-at-ucsf.edu} 7, 58 -- Organization: Microscopy Core, UCSF 7, 58 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 7, 58 -- MIME-Version: 1.0 7, 58 -- To: {Microscopy-at-microscopy.com} 7, 58 -- Subject: Who might want to work at UC San Francisco? 7, 58 -- Content-Type: text/plain; charset="utf-8"; format=flowed 7, 58 -- X-ASG-Orig-Subj: Who might want to work at UC San Francisco? 7, 58 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193] 7, 58 -- X-Barracuda-Start-Time: 1431026033 7, 58 -- X-Barracuda-Encrypted: AES128-SHA 7, 58 -- X-Barracuda-URL: https://bcuda5.ucsf.edu:443/cgi-mod/mark.cgi 7, 58 -- X-Virus-Scanned: by bsmtpd at ucsf.edu 7, 58 -- X-Barracuda-BRTS-Status: 1 7, 58 -- X-Barracuda-Spam-Score: 0.00 7, 58 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=4.5 KILL_LEVEL=5.0 tests= 7, 58 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.18705 7, 58 -- Rule breakdown below 7, 58 -- pts rule name description 7, 58 -- ---- ---------------------- -------------------------------------------------- 7, 58 -- X-CFilter-Loop: Reflected 7, 58 -- X-Brightmail-Tracker: H4sIAAAAAAAAA01Ta0hTURznbHO7Lm9dr49OSwyvlZT4Cg0jK3t80IQQYUF+yTt33Zab03tn 7, 58 -- aQStMgZaphZEkzCjfAxKzUhNKLqmTcWyIhWzhyYJaQuJDHtA9+ze2b79+D3+/985h4PJyVqV 7, 58 -- BjMV2Ri2iDZTSrUiffumw3HOh1naxJGRiNQ/fXZVOshYrJqXZYNcdZqeMZuOM2zC7jy1cbj3 7, 58 -- jbK4S11Wd6ciwA4msUoQiEEiGX6b/gpEHA5H37cpK4EaI4kpAOscLnklwAQhBdYMRom8B8CO 7, 58 -- X+OCSSXw26C9QIzGQd5dD0TLLQBvnKlRIAEnYuCzV71KhBXERvhnwe7FSiIeNgy8liFMEtFw 7, 58 -- abhRjvAaAdedbfFmw4hcWPuDl+YEw8FrswpUJ5TYABsueceECPUnOie8FjmRCq81vJCOkgbd 7, 58 -- nr9A5GNhU+O8XOQT4PKHMclDwZcX2hUijoLV7iGViHfB3/ZzEt4MJxd8WAMdTdOSPwJ6Wj8q 7, 58 -- RXwKOobdEh4D8CN/WsTr4JOWCUUNiHT6ncDpV9XpV+8GkLsAZcnXWay6E3RxYmJSfGk+V2Bh 7, 58 -- 9KZ82pzPoCePt7KGe8D76NFkN/i1vJcHBAaoIJzoOaglA+jjXLmFBwmYjArD+x5kacnVOqu+ 7, 58 -- 3EhzxqNsqZnhqFA8pkeg8RVaV2ou5AHE5II014QkPV1+kmGtYoAH6zEFtRbPHN+jJQkDbWMK 7, 58 -- GaaYYX1qCoZREN+FZgazjIEpKzCZbT5ZyJm7BYXwV8SFMiyQB3FYkLC1H1lwrpi2cCaDFAzB 7, 58 -- 96ORQT4WhYbADs1anEQ8gXhjadHKJk04zpdkaMk1fgLK+D7IFxArXFMIftY7Vfg+/1eReCEi 7, 58 -- V0kkSn0RmsmEZi40EudstM2/WQCbgZpJLPJr7CAqgRvTNXvWRz6f/F6S3WFwPlb9bT1mj3Mt 7, 58 -- HDr9dubRQOasOkV5sax+tGqg4oBj1dWl+sqnW44cgKmfra7rbs++2ZyY6kDAVzQsZ1/v6sib 7, 58 -- quKTO5vDaq+S9yfrzmfevdlWdHn+SlryhrnFkeiJne8Sf96ObOz/NOPI2d5+pTm4rIVScEY6 7, 58 -- aauc5eh/P+X2UyQEAAA= 7, 58 -- Content-Transfer-Encoding: 8bit 7, 58 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t47JIp1H003954 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Sr. Application Specialist, Bruker Berlin, Germany
Message: We are looking for an Sr. Applications Specialist to join our team in Berlin and support our energy-dispersive (EDS) and wavelength-dispersive (WDS) X-ray spectrometry products. The ideal candidate has a minimum of 3 years professional experience with EDS & WDS technologies on electron microscopes, is able to analyze complex samples of advanced materials and can produce meaningful lab reports and applications notes with minimal supervision. He/she is well connected with the scientific community, has an excellent understanding of related scientific trends, challenges and limitations, and can represent our company at scientific events and commercial tradeshows.
Go to https://worldwidecareers-bruker.icims.com/jobs/search?ss=1&searchKeyword=2723 for more information. Job ID: 2015-2723
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu May 7 19:58:09 2015 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t480w97F001204 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2015 19:58:09 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id A3048451B97 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2015 19:58:09 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at znl.com 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id ebqYqcDTSh6j for {microscopy-at-microscopy.com} ; 18, 28 -- Thu, 7 May 2015 19:58:02 -0500 (CDT) 18, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id 4B9CE451B8F 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2015 19:58:02 -0500 (CDT) 18, 28 -- Message-ID: {554C0A19.1020000-at-microscopy.com} 18, 28 -- Date: Thu, 07 May 2015 19:58:01 -0500 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: viaWWW:Sr. Application Specialist, Bruker Berlin, Germany 18, 28 -- References: {201505071645.t47GjPNi030795-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201505071645.t47GjPNi030795-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201505071645.t47GjPNi030795-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Microscopy society of NE Ohio (MSNO)
Title-Subject: [Filtered] The 59th Annual May Conference
Message: Microscopy society of NE Ohio (MSNO) with AVS,SAS and ACS is arranging the 59th Annual May meeting which will be on May 20, at John Carroll University. It is a full day meeting, including 33 talks and 30 posters. Student pre-registration is free (including lunch). MSNO member pre-registration is $25 and $35 for non-members (including technical sessions and luncheon).
You can see program and more detail at http://www.msneo.org/2015-may-meeting.html.
Wish to see you there.
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu May 7 19:59:19 2015 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t480xIme002070 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2015 19:59:18 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 8AEEA451BB2 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2015 19:59:18 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at znl.com 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id F-s9FiDW8U40 for {microscopy-at-microscopy.com} ; 16, 28 -- Thu, 7 May 2015 19:59:08 -0500 (CDT) 16, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id F0659451BAA 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2015 19:59:08 -0500 (CDT) 16, 28 -- Message-ID: {554C0A5C.4000409-at-microscopy.com} 16, 28 -- Date: Thu, 07 May 2015 19:59:08 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:The 59th Annual MSNO May Conference 16, 28 -- References: {201505072112.t47LCKgk026253-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201505072112.t47LCKgk026253-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201505072112.t47LCKgk026253-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I work with FEI Nova NanoSEM 230 equipped with Apollo 40 detector by EDAX. I have a problem concerning wrong results of EDS quantitative analysis in a standardless mode. This happens for some of our samples, such as CaF2, In2O3, CeO2, hydroxyapatite as well as for standards (from SPI supplies), such as fluorapatite, indium phosphide, etc. I realize that the standardless analysis method is not an accurate one, though the differences between real and measured values seem to be too large (up to about 10%). For example, fluorapatite contains 38,6 wt% of Ca, while the measured value for Ca is 28,9 wt%. I tried to find a solution for the problem. I made a calibration using Cu and Al K lines. I checked HPD, but there was a good fit between measured and theoretical spectra. I was changing various parameters, such as working distance, spot size, acceleration voltage, time constant, but differences between real and measured values were roughly remaining the same. Next, I tried to use SEC parameters correction. For example, based on the In2O3 and InP, I set SEC value for phosphor. Nevertheless, this deteriorated the results of quantitative analysis for a pentaphosphate. It seems that SEC correction is not a sufficient solution. Finally, I tried measurement using our old and rarely used Philips SEM 515 microscope equipped with SUTW+ detector (also by EDAX) cooled with liquid nitrogen. Surprisingly, results of quantitative analysis for hydroxyapatite turned out to be almost correct. This suggests a problem with the newer Apollo detector. Does the detector work wrong? Is there any solution to this problem, which can be found by a user?
Krzysztof Rola
Institute of Low Temperature and Structural Research Polish Academy of Sciences Wroclaw, Poland
==============================Original Headers============================== 5, 18 -- From k.rola-at-int.pan.wroc.pl Fri May 8 08:59:16 2015 5, 18 -- Received: from mserv3.int.pan.wroc.pl (mserv3.int.pan.wroc.pl [156.17.85.6]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t48DxFU5013187 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 8 May 2015 08:59:16 -0500 5, 18 -- Received: from [192.168.25.130] (unknown [192.168.25.130]) 5, 18 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 5, 18 -- (No client certificate requested) 5, 18 -- by mserv3.int.pan.wroc.pl (Postfix) with ESMTPSA id B5F124232F 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 8 May 2015 15:59:14 +0200 (CEST) 5, 18 -- Message-ID: {554CC133.7060708-at-int.pan.wroc.pl} 5, 18 -- Date: Fri, 08 May 2015 15:59:15 +0200 5, 18 -- From: "K.Rola" {k.rola-at-int.pan.wroc.pl} 5, 18 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 5, 18 -- MIME-Version: 1.0 5, 18 -- To: Microscopy-at-Microscopy.Com 5, 18 -- Subject: SEM/EDS - problem with standardless quantitative analysis 5, 18 -- Content-Type: text/plain; charset=utf-8; format=flowed 5, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, I need to work with fresh human or human-analog material concerning the structure of the eye. Any procedure for fixation, OsO4 staining and going with ethanol to critical point drying would be appreciated. Tissues to be processed are lens, iris and retina.
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
We are looking to employ a Technical Manger for the Neuroscience Imaging Center at the University of Tennessee Health Science Center, based in Memphis TN.
This position will manage the Neuroscience Imaging Center at UTHSC, which is comprised of five major facilities for on-campus and off-campus users who desire training in light, confocal, and electron microscopic imaging. Duties and responsibilities include training new users on Imaging Center equipment; assisting users with experimental design; sample processing and sectioning for TEM; overseeing scheduling of all major equipment; billing and ordering of necessary supplies; determining workload for Imaging Center histology technician.
Minimum qualifications are M.A. or M.S. degree with three (3) years of experience directly related to the duties stated above. Candidate should have knowledge of cell biology (preferably some neuroscience) and histology; knowledge of embedding and sectioning material for light, confocal and electron microscopy, operation and theory of light, confocal and electron microscopes; excellent interpersonal, verbal, and written communication skills. For a full job description, please follow this link:
I would also be happy to answer any questions regarding this position.
Sincerely,
Amanda
-- Amanda Preston, Ph.D Technical Manager Neuroscience Imaging Center University of Tennessee Health Science Center 311 Link Building Memphis, TN 38163 (901) 448-5976 apresto9-at-uthsc.edu
==============================Original Headers============================== 14, 42 -- From apresto9-at-uthsc.edu Fri May 8 14:09:11 2015 14, 42 -- Received: from barracuda.uthsc.edu (smtp.uthsc.edu [128.169.0.28]) 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t48J9B9D030254 14, 42 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2015 14:09:11 -0500 14, 42 -- X-ASG-Debug-ID: 1431112149-04e3fa1c83d1650003-4CH8be 14, 42 -- Received: from hscch1.uthsc.tennessee.edu (hscch1.uthsc.edu [128.169.10.80]) by barracuda.uthsc.edu with ESMTP id RNld0o3yw3C35teX (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Fri, 08 May 2015 14:09:10 -0500 (CDT) 14, 42 -- X-Barracuda-Envelope-From: apresto9-at-uthsc.edu 14, 42 -- X-Barracuda-Apparent-Source-IP: 128.169.10.80 14, 42 -- Received: from HSCMBX5.uthsc.tennessee.edu ([169.254.3.154]) by 14, 42 -- hscch1.uthsc.tennessee.edu ([128.169.10.80]) with mapi id 14.03.0224.002; 14, 42 -- Fri, 8 May 2015 14:06:50 -0500 14, 42 -- From: "Preston, Amanda M" {apresto9-at-uthsc.edu} 14, 42 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 42 -- Subject: Technical Manager - Memphis TN 14, 42 -- Thread-Topic: Technical Manager - Memphis TN 14, 42 -- X-ASG-Orig-Subj: Technical Manager - Memphis TN 14, 42 -- Thread-Index: AQHQicIiyjbZwylG406uElh1vWHvRQ== 14, 42 -- Date: Fri, 8 May 2015 19:06:49 +0000 14, 42 -- Message-ID: {D1727378.1176E%apresto9-at-uthsc.edu} 14, 42 -- Accept-Language: en-US 14, 42 -- Content-Language: en-US 14, 42 -- X-MS-Has-Attach: 14, 42 -- X-MS-TNEF-Correlator: 14, 42 -- user-agent: Microsoft-MacOutlook/14.4.4.140807 14, 42 -- x-originating-ip: [172.21.160.137] 14, 42 -- Content-Type: text/plain; charset="us-ascii" 14, 42 -- Content-ID: {7D9A64C3F56F3345B9B9FA54409D5095-at-mail.tennessee.edu} 14, 42 -- MIME-Version: 1.0 14, 42 -- X-Barracuda-Connect: hscch1.uthsc.edu[128.169.10.80] 14, 42 -- X-Barracuda-Start-Time: 1431112150 14, 42 -- X-Barracuda-Encrypted: AES128-SHA 14, 42 -- X-Barracuda-URL: http://128.169.0.28:8000/cgi-mod/mark.cgi 14, 42 -- X-Virus-Scanned: by bsmtpd at uthsc.edu 14, 42 -- X-Barracuda-BRTS-Status: 1 14, 42 -- X-Barracuda-Spam-Score: 0.00 14, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=5.0 tests= 14, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.18732 14, 42 -- Rule breakdown below 14, 42 -- pts rule name description 14, 42 -- ---- ---------------------- -------------------------------------------------- 14, 42 -- Content-Transfer-Encoding: 8bit 14, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t48J9B9D030254 ==============================End of - Headers==============================
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Email: rhsia-at-umaryland.edu Name: Ru-ching Hsia
Organization: University of Maryland Baltimore, Electron Microscopy Core Imaging Facility
Title-Subject: [Filtered] UMB Current EM Techniques workshop and Chesapeake Microscopy &Micoranalysis Society Spring Dinner
Message: UMB and Chesapeake Microscopy & Microanalysis Society Current Electron Microscopy Techniques Workshop and Spring Dinner
May 28th and 29th, 2015.
Dear Colleagues,
This is to remind you that registration for the UMB/CMMS Current EM Techniques Workshop and Spring Dinner will close on May 21st, 2015, 5 PM.
This yearÂs workshop will be focused on immuno electron microscopy (IEM). The format includes oral presentations, instrument demonstrations and Tips-and-Tricks discussion forums. Dr. Kent McDonald from UC Berkeley will be keynote speaker this year. Other speakers include application specialists from Carl Zeiss Microscopy, Aurion and Nanoprobes. Demo instruments will feature the high pressure freezer (Leica EMPACT 2), automated and quick freeze substitution systems, cryo-ultramicrotome, grid plunger, cryo-TEM, cryo-SEM and CorrSight CLEM solution. There will be four Tips-and-Tricks discussion forum sessions led by experienced panelists to discuss various aspects of pre-embedding, post-embedding, Tokuyasu immunolabeling techniques and alternative non-antibody labeling. The Chesapeake Microscopy and Microanalysis Society (CMMS) Spring Dinner will be held on the evening of May 28th providing opportunities for social and scientific exchange among workshop participants and CMMS members.
More information regarding the workshop and registration can be found on our website http://www.dental.umaryland.edu/2015currentemtechniquesworkshop/
Please email at coreimaging-at-umaryland.edu for any inquiries about the workshop. We look forward to welcoming you in Baltimore soon.
Electron Microscopy Core Imaging Facility University of Maryland, Baltimore
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==============================Original Headers============================== 18, 29 -- From microscopylistserver-noreply-at-microscopy.com Fri May 8 16:54:00 2015 18, 29 -- Received: from znl.com ([206.69.208.20]) 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t48Ls0rs021212 18, 29 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2015 16:54:00 -0500 18, 29 -- Received: from localhost (localhost [127.0.0.1]) 18, 29 -- by znl.com (Postfix) with ESMTP id 63155454B33 18, 29 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2015 16:54:00 -0500 (CDT) 18, 29 -- X-Virus-Scanned: amavisd-new at znl.com 18, 29 -- Received: from znl.com ([127.0.0.1]) 18, 29 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 18, 29 -- with ESMTP id axfRvsGmhk_W for {microscopy-at-microscopy.com} ; 18, 29 -- Fri, 8 May 2015 16:53:53 -0500 (CDT) 18, 29 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 18, 29 -- by znl.com (Postfix) with ESMTPA id 04C9F454B25 18, 29 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2015 16:53:53 -0500 (CDT) 18, 29 -- Message-ID: {554D3070.8040206-at-microscopy.com} 18, 29 -- Date: Fri, 08 May 2015 16:53:52 -0500 18, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 18, 29 -- MIME-Version: 1.0 18, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 29 -- Subject: viaWWW:UMB Current EM Techniques workshop and Chesapeake Microscopy 18, 29 -- &Micoranalysis Society Spring Dinner 18, 29 -- References: {201505081443.t48EhqEl002691-at-ns.microscopy.com} 18, 29 -- In-Reply-To: {201505081443.t48EhqEl002691-at-ns.microscopy.com} 18, 29 -- X-Forwarded-Message-Id: {201505081443.t48EhqEl002691-at-ns.microscopy.com} 18, 29 -- Content-Type: text/plain; charset=UTF-8; format=flowed 18, 29 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dst-at-gmail.com Sat May 9 00:43:14 2015 Return-Path: {mike.sfsd4f564s6df45dst-at-gmail.com} Received: from gmail.com ([112.216.97.198]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t495h8mt017187 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 9 May 2015 00:43:13 -0500 Message-ID: {7DF1C7F7.BB129397-at-gmail.com}
X-from: Pamela-at-afmworkshop.com
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Email: Pamela-at-afmworkshop.com Name: Pamela Stone
Organization: AFMWorkshop
Title-Subject: [Filtered] AFM Bio Training Course
What: Workshop: Bioapplications with Atomic Force Microscopy When: July 16-17 Where: Signal Hill, CA
Atomic force microscopes (AFMs) enable advanced measurements of biological samples that are not possible with other microscopy techniques. These include measuring high resolution images of biomolecules and cells in ambient as well as in situ liquid environments. In addition to measuring images, an AFM can capture force/displacement curves that give a quantitative measurement of a specimen's stiffness or of molecular interaction forces.
This two day course provides the operational concepts of an atomic force microscope as well as hands-on AFM =C2=AD experience. Morning classes cover the range of AFM bioapplications, while afternoon labs provide applications training using the TT-AFM as well as the LS-AFM.
Biological topics covered in the course: - Sample preparation for biological experiments - Imaging biological samples in air and in liquid - Measuring Force-Distance curves for sample stiffness or intermolecular force determination
Attendees with all levels of AFM experience, and owners of all makes and models of AFMs, are welcome.
For more information, please visit: http://www.afmworkshop.com/afm-bioapplications.html
Thank you, Pamela Stone AFMWorkshop, Inc.
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==============================Original Headers============================== 21, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat May 9 18:14:05 2015 21, 28 -- Received: from znl.com ([206.69.208.20]) 21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t49NE5S5023683 21, 28 -- for {microscopy-at-microscopy.com} ; Sat, 9 May 2015 18:14:05 -0500 21, 28 -- Received: from localhost (localhost [127.0.0.1]) 21, 28 -- by znl.com (Postfix) with ESMTP id 64EB1458448 21, 28 -- for {microscopy-at-microscopy.com} ; Sat, 9 May 2015 18:14:05 -0500 (CDT) 21, 28 -- X-Virus-Scanned: amavisd-new at znl.com 21, 28 -- Received: from znl.com ([127.0.0.1]) 21, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 21, 28 -- with ESMTP id IUYwPDG-7qk2 for {microscopy-at-microscopy.com} ; 21, 28 -- Sat, 9 May 2015 18:13:55 -0500 (CDT) 21, 28 -- Received: from mac22.zaluzec.com (unknown [206.69.208.22]) 21, 28 -- by znl.com (Postfix) with ESMTPA id 8D00C45843B 21, 28 -- for {microscopy-at-microscopy.com} ; Sat, 9 May 2015 18:13:55 -0500 (CDT) 21, 28 -- Message-ID: {554E94B2.3090405-at-microscopy.com} 21, 28 -- Date: Sat, 09 May 2015 18:13:54 -0500 21, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 21, 28 -- MIME-Version: 1.0 21, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 28 -- Subject: viaWWW:Workshop: Bioapplications with Atomic Force Microscopy 21, 28 -- References: {201505091746.t49Hkoul006852-at-ns.microscopy.com} 21, 28 -- In-Reply-To: {201505091746.t49Hkoul006852-at-ns.microscopy.com} 21, 28 -- X-Forwarded-Message-Id: {201505091746.t49Hkoul006852-at-ns.microscopy.com} 21, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 21, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: P.J.F.Harris-at-reading.ac.uk Name: Peter Harris
Organization: University of Reading
Title-Subject: [Filtered] Patchy TEM negatives
Message: We are still using photographic film to record TEM images, and we have a persistent problem with the negatives coming out very patchy and uneven. Sometimes this obscures detail in the image. Apart from "buy a high-res digital camera", does anyone have any suggestions?
Many thanks
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon May 11 08:09:05 2015 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4BD95xO028177 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2015 08:09:05 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 067C845D970 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2015 08:09:05 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at znl.com 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id 96L3Bi7HU5Zf for {microscopy-at-microscopy.com} ; 13, 28 -- Mon, 11 May 2015 08:08:51 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id A53A645D968 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2015 08:08:51 -0500 (CDT) 13, 28 -- Message-ID: {5550A9E3.8090609-at-microscopy.com} 13, 28 -- Date: Mon, 11 May 2015 08:08:51 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Patchy TEM negatives 13, 28 -- References: {201505111044.t4BAi5YV022387-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201505111044.t4BAi5YV022387-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201505111044.t4BAi5YV022387-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Wow, this takes me back to my time as a Hitachi demonstrator in the 1970s! We had Ilford as a customer, and one thing that they taught me, that helped me past this problem, was to follow the "Dunk and Tilt" procedure.
1. Submerge the film in the developer and tap the rack on the tank base (to remove air bubbles). 2. After 15secs lift out the film and tilt to one side for 2secs. 3. Repeat the procedure, including tapping, tilting to alternates sides as you pass through the development cycle. 4. Place in running water for one minute. 5. Follow exactly the same procedure in the fix, for the for the first one and a half minutes, before allowing the fixation to be completed. 6. Wash in running water for 30 minutes, with the drain above the level of the film. 7. Immerse in a wetting agent, a few drops of soap solution in a developing tank full of water. 8. Dry in a cabinet with the temperature no higher than 100deg F.
The only further problem I had to solve was fogging of the negatives, because I did not know that darkroom filters only have a specific "safe" life! This problem is generated by the amount of time the film spends out in the dark room as it is being processed.
I used a point source enlarger that gave amazing contrast, but equally amazing could see even the most subtle patches within the negatives. Following the above solved my problem and went a long way in helping Hitachi secure TEM orders.
Good Luck
Steve
Steve Chapman FRMS Electron Microscopy Consultancy and Training www.emcourses.com Cell +44 (0)7711606967
---------------------------------------------------------------------------- Email: P.J.F.Harris-at-reading.ac.uk Name: Peter Harris
Organization: University of Reading
Title-Subject: [Filtered] Patchy TEM negatives
Message: We are still using photographic film to record TEM images, and we have a persistent problem with the negatives coming out very patchy and uneven. Sometimes this obscures detail in the image. Apart from "buy a high-res digital camera", does anyone have any suggestions?
Many thanks
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon May 11 08:09:05 2015 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4BD95xO028177 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2015 08:09:05 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 067C845D970 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2015 08:09:05 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at znl.com 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id 96L3Bi7HU5Zf for {microscopy-at-microscopy.com} ; 13, 28 -- Mon, 11 May 2015 08:08:51 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id A53A645D968 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2015 08:08:51 -0500 (CDT) 13, 28 -- Message-ID: {5550A9E3.8090609-at-microscopy.com} 13, 28 -- Date: Mon, 11 May 2015 08:08:51 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Patchy TEM negatives 13, 28 -- References: {201505111044.t4BAi5YV022387-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201505111044.t4BAi5YV022387-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201505111044.t4BAi5YV022387-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 21, 22 -- From protrain-at-emcourses.com Mon May 11 08:56:32 2015 21, 22 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 21, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4BDuW3X017042 21, 22 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2015 08:56:32 -0500 21, 22 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 21, 22 -- id {B5550b50f0000} ; Mon, 11 May 2015 14:56:31 +0100 21, 22 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Mon, 11 May 2015 14:56:30 +0100 21, 22 -- From: "Steve Chapman" {protrain-at-emcourses.com} 21, 22 -- To: {P.J.F.Harris-at-reading.ac.uk} 21, 22 -- Cc: {microscopy-at-microscopy.com} 21, 22 -- References: {201505111311.t4BDBwSR029400-at-ns.microscopy.com} 21, 22 -- In-Reply-To: {201505111311.t4BDBwSR029400-at-ns.microscopy.com} 21, 22 -- Subject: RE: [Microscopy] viaWWW:Patchy TEM negatives 21, 22 -- Date: Mon, 11 May 2015 14:56:27 +0100 21, 22 -- Message-ID: {000601d08bf2$46941240$d3bc36c0$-at-com} 21, 22 -- MIME-Version: 1.0 21, 22 -- Content-Type: text/plain; 21, 22 -- charset="us-ascii" 21, 22 -- Content-Transfer-Encoding: 7bit 21, 22 -- X-Mailer: Microsoft Office Outlook 12.0 21, 22 -- Thread-Index: AdCL7AqXR9dDG55lS3y2jBhQ1XE38AAAyqXw 21, 22 -- Content-Language: en-gb ==============================End of - Headers==============================
Hi, as Steve suggests, even development needs agitation. What you can do prior to the developer to enhance even development is: - immerse your rack with the films into plain water with the same temeprature as the developer for one minute, agitate and tap films to the tank base to remove air bubble adhering to the films - then put rack into the developer, add ca. 30-40 seconds more time than usual to allow for replacement of the water in the film with developer
- rinse - fix etc.
Foggy negatives can also result from a weak developer, exhausted fixer. Are you sure solutions are still good? Be sure to use an active stop bath (3% acetic acid) before fixer. Water might not be good enough for this job...
Sometimes the problem is the film. You can contact me offline with the name of the producer ;-)
Best, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 11.05.15 um 15:20 schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: P.J.F.Harris-at-reading.ac.uk } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both P.J.F.Harris-at-reading.ac.uk as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: P.J.F.Harris-at-reading.ac.uk } Name: Peter Harris } } Organization: University of Reading } } Title-Subject: [Filtered] Patchy TEM negatives } } Message: We are still using photographic film to record TEM images, and we have a persistent problem } with the negatives coming out very patchy and uneven. Sometimes this obscures detail in the image. } Apart from "buy a high-res digital camera", does anyone have any suggestions? } } Many thanks } } Login Host: 134.225.49.100 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 11, 23 -- From stefan.diller-at-t-online.de Mon May 11 09:24:37 2015 11, 23 -- Received: from mailout09.t-online.de (mailout09.t-online.de [194.25.134.84]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4BEOakV005736 11, 23 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2015 09:24:36 -0500 11, 23 -- Received: from fwd11.aul.t-online.de (fwd11.aul.t-online.de [172.20.27.152]) 11, 23 -- by mailout09.t-online.de (Postfix) with SMTP id 637691414D1; 11, 23 -- Mon, 11 May 2015 16:24:35 +0200 (CEST) 11, 23 -- Received: from mac-pro.local (TJ95cUZrrhQEOavsvISdZ+kZX5O4yaGv69J2kp8ZOBePFh8iF3w1nJQlTwxNGjqw3O-at-[91.58.188.222]) by fwd11.t-online.de 11, 23 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) 11, 23 -- esmtp id 1YrodF-1L0IIi0; Mon, 11 May 2015 16:24:33 +0200 11, 23 -- Message-ID: {5550BBA1.4060104-at-t-online.de} 11, 23 -- Date: Mon, 11 May 2015 16:24:33 +0200 11, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 11, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 11, 23 -- MIME-Version: 1.0 11, 23 -- To: microscopy-at-microscopy.com, P.J.F.Harris-at-reading.ac.uk 11, 23 -- Subject: Re: [Microscopy] viaWWW:Patchy TEM negatives 11, 23 -- References: {201505111320.t4BDKFjW001438-at-ns.microscopy.com} 11, 23 -- In-Reply-To: {201505111320.t4BDKFjW001438-at-ns.microscopy.com} 11, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 23 -- Content-Transfer-Encoding: 7bit 11, 23 -- X-ID: TJ95cUZrrhQEOavsvISdZ+kZX5O4yaGv69J2kp8ZOBePFh8iF3w1nJQlTwxNGjqw3O 11, 23 -- X-TOI-MSGID: 2dd663bf-4f0d-4683-9e80-fe8d68fb8950 ==============================End of - Headers==============================
From mike.newsletter30ujgyl-at-gmail.com Mon May 11 14:35:24 2015 Return-Path: {mike.newsletter30ujgyl-at-gmail.com} Received: from gmail.com ([1.210.175.62]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t4BJZKW1008102 for {microscopylistserverarchive5-at-microscopy.com} ; Mon, 11 May 2015 14:35:22 -0500 Message-ID: {9E4593BE.80574DF0-at-gmail.com}
On May 11, 2015, at 6:36 AM, microscopylistserver- noreply-at-microscopy.com wrote:
} } } } Email: P.J.F.Harris-at-reading.ac.uk } Name: Peter Harris } } Organization: University of Reading } } Title-Subject: [Filtered] Patchy TEM negatives } } Message: We are still using photographic film to record TEM images, } and we have a persistent problem } with the negatives coming out very patchy and uneven. Sometimes this } obscures detail in the image. } Apart from "buy a high-res digital camera", does anyone have any } suggestions? } } Many thanks }
Dear Peter, If you use bubbling N2 gas to agitate the developer, uneven exposure to the developer can occur. Try to use either more or less agitation until the patchiness goes away, or agitate by moving the negative rack up and down in the tank. If there are limited areas that are uneven, you can sometimes fix that during printing. N.b., this requires practice to develop the skill required, but if the first print is not satisfactory, try another. Good luck.
Yours, Bill
==============================Original Headers============================== 8, 32 -- From wtivol-at-sbcglobal.net Mon May 11 20:45:28 2015 8, 32 -- Received: from nm22-vm6.access.bullet.mail.gq1.yahoo.com (nm22-vm6.access.bullet.mail.gq1.yahoo.com [216.39.63.170]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4C1jPdg002660 8, 32 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2015 20:45:27 -0500 8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1431395125; bh=LxfIaZxrVbT8h8HbJkoeW3cKYPeS+FCsCWtY4nwwaRU=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=dx8ffCp/kHyzni+rm0itJbKtCsj0CZWYGjWlXrwzXk7C/Esx7LiWJ2dxvsOXNYVbLp1qI7z58sLFsvPt0ZX7dgb8VWn9H8Cb87r7a46z5sjyBJikMSEC9h4j9o3Vb61B5SDUS/ZBLw6O3/KdP+TMz9aqBNIJiIU7hgVMBAcjd1yhAKHRH1pZKI0nlomAzPgKC3Fx/yK2cplqn5dlVeea6g4dc+n+NxSTlO76byJ62I4tdl4rmQr5iJifJPXhkpy3Q/EuNpQLryWKlPYka0+3GI0O+Ewhh8rCNMSJebZ87r5whQrJs5oT6XQX1XF0pF/wrBN6V58445foCnHuc2xlVg== 8, 32 -- Received: from [216.39.60.170] by nm22.access.bullet.mail.gq1.yahoo.com with NNFMP; 12 May 2015 01:45:25 -0000 8, 32 -- Received: from [67.195.23.145] by tm6.access.bullet.mail.gq1.yahoo.com with NNFMP; 12 May 2015 01:45:25 -0000 8, 32 -- Received: from [127.0.0.1] by smtp117.sbc.mail.gq1.yahoo.com with NNFMP; 12 May 2015 01:45:25 -0000 8, 32 -- X-Yahoo-Newman-Id: 191367.88793.bm-at-smtp117.sbc.mail.gq1.yahoo.com 8, 32 -- X-Yahoo-Newman-Property: ymail-3 8, 32 -- X-YMail-OSG: hdLMkGkVM1lc.Dd0taetYgkIiUCdY38xQp3HWxRfViPYGrR 8, 32 -- _HqYT9Y9GRVwWdJiUAMarSJ4pAiNhqdRHFVjhqUFP9jZpO7byJYSZg6rJ.VU 8, 32 -- Yit_z8kS4MUCFVqmAn1WGViyonHfXZKg3r2DH9n_nfuh38w6hoQNvDledyKv 8, 32 -- 0oc3nPPQYc8I7b3.I1iY3rNlgi6nun8QXZoD6.vnYY32M37A5DqtbJHINRG. 8, 32 -- .t63KEA.PIDcLjpWi8LzmLhb1.eOdOgDRJHgB5O2GvRaJmyiO70KvMMNr6kD 8, 32 -- RU4RU33CB4efTAGzoVDnd0cBOTRPxN0DqEPN5g4uqHd3zjCS0iKBFVKhMiey 8, 32 -- Pzj0IGgj.O9kZS4P9YYXI8Sc4B7NtUdWovga0ifTNA9ymGdS6NHsP_QTuH7c 8, 32 -- 0mgSSdcP42Rec9J6iaaDbPwJ2fLjVbIQO_LfuwDlfn_u9lF3F.mWUAb238gv 8, 32 -- vdPkxrf3.PN9YJTP_zEeSv3fWFuds_9wUW3g0.cwIf1GXYLMU5sHLk2iL.Db 8, 32 -- 4USi5NQTNSev1MnBmHhEh06M6oaPtrv3wH5Sz1Bo- 8, 32 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 8, 32 -- Message-Id: {3B3CBAF8-D6DC-4790-9A57-285A5C0E497C-at-sbcglobal.net} 8, 32 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 8, 32 -- To: microscopy-at-microscopy.com, P.J.F.Harris-at-reading.ac.uk 8, 32 -- In-Reply-To: {201505111336.t4BDai0g010828-at-ns.microscopy.com} 8, 32 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 32 -- Content-Transfer-Encoding: 7bit 8, 32 -- Mime-Version: 1.0 (Apple Message framework v936) 8, 32 -- Subject: Re: [Microscopy] viaWWW:Patchy TEM negatives 8, 32 -- Date: Mon, 11 May 2015 18:45:23 -0700 8, 32 -- References: {201505111336.t4BDai0g010828-at-ns.microscopy.com} 8, 32 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
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Email: chris.guerin-at-irc.vib-ugent.be Name: Christopher Guerin
Organization: VIB
Title-Subject: [Filtered] post doctoral position in Ghent Belgium
Message: Hi Everyone:
A 2 year post-doctoral fellowship is available immediately at the VIB Bio Imaging Core in Ghent Belgium. This position is designed for a person who will work on a joint project with Janssen Pharmaceutial Group of Companies (J&J) in Beerse, Belgium and the successful candidate will spend most of their time on the Beerse site.
The project will involve the development of high throughput microscopy for automated imaging of mouse brain slices and analysis of phenotypic changes in models of neurodegenerative disease. Additional partners will provide engineering and computational image analysis expertise. The fellow will be responsible for all aspects of the microscopy and imaging, but will be expected to work closely together with the computational scientist to explore and validate the image and data analyses. Profile
you have a degree in biological sciences you have some experience of microscopy experience in or knowledge of mouse neuroanatomy are a plus recent graduates are welcome to apply you are an interactive individual fluency in English is required
We offer:
a renewable 2-year contract a competitive salary a position at the interface between industry and the academic world experience in translational research excellent skills and training courses
The post will remain open until filled and preference will be given to candidates that can begin before end of June 2015.
To apply please go to the VIB jobs webpage at: http://www.vib.be/en/jobs/Pages/Postdoc-Position-at-VIB-Bio-Imaging.aspx
Informal enquiries can be made directly to me at chris.guerin-at-irc.vib-ugent.be
Best regards,
Chris
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==============================Original Headers============================== 24, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue May 12 07:43:24 2015 24, 28 -- Received: from znl.com ([206.69.208.20]) 24, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4CChNmH004777 24, 28 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2015 07:43:23 -0500 24, 28 -- Received: from localhost (localhost [127.0.0.1]) 24, 28 -- by znl.com (Postfix) with ESMTP id E8CDC460ED1 24, 28 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2015 07:43:22 -0500 (CDT) 24, 28 -- X-Virus-Scanned: amavisd-new at znl.com 24, 28 -- Received: from znl.com ([127.0.0.1]) 24, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 24, 28 -- with ESMTP id CYtJAq5vGLx0 for {microscopy-at-microscopy.com} ; 24, 28 -- Tue, 12 May 2015 07:43:15 -0500 (CDT) 24, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 24, 28 -- by znl.com (Postfix) with ESMTPA id 1EB4B460EC5 24, 28 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2015 07:43:15 -0500 (CDT) 24, 28 -- Message-ID: {5551F562.1070600-at-microscopy.com} 24, 28 -- Date: Tue, 12 May 2015 07:43:14 -0500 24, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 24, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 24, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 24, 28 -- MIME-Version: 1.0 24, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 24, 28 -- Subject: viaWWW:post doctoral position in Ghent Belgium 24, 28 -- References: {201505120844.t4C8iVrb032301-at-ns.microscopy.com} 24, 28 -- In-Reply-To: {201505120844.t4C8iVrb032301-at-ns.microscopy.com} 24, 28 -- X-Forwarded-Message-Id: {201505120844.t4C8iVrb032301-at-ns.microscopy.com} 24, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 24, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Quick, I need to know in the next 2.5 hours (it's still afternoon here in Hawaii):
Can LR White be polymerized in plastic Petri dishes? I don't know why I don't know this. I've always used gelatin capsules, but these cells need to be fixed and preferably embedded in place. Will it polymerize under a vacuum at 50C? How about with UV in the freezer? I'd prefer the former...
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Tue May 12 19:58:55 2015 4, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4D0wsOs017863 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2015 19:58:55 -0500 4, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id t4D0wsX5011614 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2015 14:58:54 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id t4D0wrGR011610 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2015 14:58:53 -1000 (HST) 4, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Tue, 12 May 2015 14:58:53 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-b1000 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: Can LR White be polymerized in plastic Petri dishes? 4, 20 -- Message-ID: {Pine.GSO.4.64.1505121456270.7442-at-b1000} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
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Organization: Centers for Disease Control and Prevention
Title-Subject: [Filtered] Disappearing glycogen
Message: For several years, we have lost the ability to visualize glycogen by thin section TEM. As I recall, this occurred when we changed bottles of DBP in our Epon-substitute/Araldite mixture. Has anyone else had the glycogen ÂdisappearÂ, and come up with a solution?
Thank you, Cynthia Goldsmith Infectious Diseases Pathology Branch Centers for Disease Control and Prevention
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue May 12 20:16:46 2015 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4D1Gkn5027947 12, 28 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2015 20:16:46 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id 30162462B23 12, 28 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2015 20:16:46 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at znl.com 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id 8HAE6ekt6W9D for {microscopy-at-microscopy.com} ; 12, 28 -- Tue, 12 May 2015 20:16:38 -0500 (CDT) 12, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id CBF0D462B15 12, 28 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2015 20:16:38 -0500 (CDT) 12, 28 -- Message-ID: {5552A5F5.4030806-at-microscopy.com} 12, 28 -- Date: Tue, 12 May 2015 20:16:37 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Disappearing glycogen 12, 28 -- References: {201505122138.t4CLcq9q007773-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201505122138.t4CLcq9q007773-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201505122138.t4CLcq9q007773-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 12, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
As a reminder - 4 days remain to submit your abstract to the workshop "Big, Deep, and Smart Data Analytics in Materials Imaging" organized jointly by the five DOE Nanoscale Science Research Centers (Program Committee: E. Stach, J. Werner, D. Miller, S.V. Kalinin and J. Schuck), to be held at Oak Ridge National Laboratory on June 8-10.
This workshop brings together researchers from different imaging disciplines (electron microscopy, scanning probe microscopy, focused x-ray, neutron, atom probe tomography, chemical imaging, optical microscopies) with the experts in mathematical/statistical/computational approaches to discuss opportunities and future needs in the integration of advanced data analytics and theory into imaging science. It will provide a forum to present achievements in the various imaging disciplines with emphasis on acquisition, visualization, and analysis of multidimensional data sets, the corresponding approaches for theory-experiment matching, and novel opportunities for instrumental development enabled by the availability of high speed data analytic tools. Additional information regarding this workshop can be found at http://www.cnms.ornl.gov/JointNSRC2015/. The confirmed invited speakers include Paul Voyles (UWisc), James Labeau (NCSU), Eric Stach (BNL), Michael Demkowicz (MIT), Jim Ciston (Berkeley), Kirsten Kleese van Dam (PNNL), Paul Kotula (Sandia), and many others.
Please note that the abstract deadline (May 17) and registration deadline (May 25) are coming up soon. Looking forward to seeing you at ORNL!
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Theme Leader, Center for Nanophase Materials Science
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 10, 24 -- From sergei2-at-ornl.gov Wed May 13 08:19:55 2015 10, 24 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.14]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4DDJsgu022534 10, 24 -- for {microscopy-at-microscopy.com} ; Wed, 13 May 2015 08:19:54 -0500 10, 24 -- X-SG: RELAYLIST 10, 24 -- X-IronPort-AV: E=Sophos;i="5.13,420,1427774400"; 10, 24 -- d="scan'208";a="102845404" 10, 24 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 10, 24 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 13 May 2015 09:19:53 -0400 10, 24 -- Received: from [128.219.192.92] (pc83680.ornl.gov [128.219.192.92]) 10, 24 -- (using TLSv1 with cipher DHE-RSA-CAMELLIA256-SHA (256/256 bits)) 10, 24 -- (No client certificate requested) 10, 24 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id A5F6F100303; 10, 24 -- Wed, 13 May 2015 09:19:52 -0400 (EDT) 10, 24 -- Message-ID: {55534F78.8000500-at-ornl.gov} 10, 24 -- Date: Wed, 13 May 2015 09:19:52 -0400 10, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 10, 24 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:14.0) Gecko/20120713 Thunderbird/14.0 10, 24 -- MIME-Version: 1.0 10, 24 -- To: undisclosed-recipients:; 10, 24 -- Subject: Reminder - Workshop on Big, Deep, and Smart Data Analytics in Materials 10, 24 -- Imaging 10, 24 -- Content-Type: text/plain; charset=UTF-8; format=flowed 10, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Cynthia, Glycogen can become coalesced into amorphous unstained regions in the cytoplasm under certain fixing/staining conditions. I have found this in certain cell types (ie muscle, lung tissue) if you use non-reduced osmium followed by un-buffered uranyl acetate (aqueous) en-bloc staining. Generally if you use non-reduced osmium, I would en-bloc with buffered (0.1 M maleate pH 6.2) 2% uranyl acetate. If you do use reduced osmium (K4Fe(CN)6), then you can continue with aqueous uranyl acetate. A remedy for material already in plastic is to triple stain the sections: 1% filtered tannic acid (10 min) followed by 2% uranyl acetate ( aq.) then lead citrate. This will fill in the amorphous regions and stain the glycogen there, (I have done this with lung tissue). Hope this helps; Michael Delannoy
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Organization: Centers for Disease Control and Prevention
Title-Subject: [Filtered] Disappearing glycogen
Message: For several years, we have lost the ability to visualize glycogen by thin section TEM. As I recall, this occurred when we changed bottles of DBP in our Epon-substitute/Araldite mixture. Has anyone else had the glycogen ÂdisappearÂ, and come up with a solution?
Thank you, Cynthia Goldsmith Infectious Diseases Pathology Branch Centers for Disease Control and Prevention
==============================Original Headers============================== 15, 21 -- From prvs=56882261b=mdelann1-at-jhmi.edu Wed May 13 10:35:52 2015 15, 21 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.200]) 15, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4DFZpjx015602 15, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 10:35:52 -0500 15, 21 -- X-IronPort-AV: E=Sophos;i="5.13,421,1427774400"; 15, 21 -- d="scan'208";a="35790933" 15, 21 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96]) 15, 21 -- by IPEB3.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 13 May 2015 11:35:51 -0400 15, 21 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 15, 21 -- To: {Microscopy-at-microscopy.com} 15, 21 -- Subject: glycogen 15, 21 -- Date: Wed, 13 May 2015 11:35:54 -0400 15, 21 -- Message-ID: {000101d08d92$7ff88740$7fe995c0$-at-jhmi.edu} 15, 21 -- MIME-Version: 1.0 15, 21 -- Content-Type: text/plain; 15, 21 -- charset="iso-8859-1" 15, 21 -- X-Mailer: Microsoft Outlook 14.0 15, 21 -- Thread-Index: AdCNkm3bumACbZYsQ9S6wTV22+H75w== 15, 21 -- Content-Language: en-us 15, 21 -- Content-Transfer-Encoding: 8bit 15, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4DFZpjx015602 ==============================End of - Headers==============================
Thanks to everyone who replied. It was late for most of you ... we needed to decide before 6:00 pm Hawaii time whether to fix these cells in place in plastic Petri dishes or to detach them before fixation (which they don't like). We went ahead and fixed in place, so I will have an answer for any interested parties next week. I will try to exclude air with Aclar (if I can find my stash) or something else. Maybe also pull a vacuum.
The replies included 2 "No, not ever!", 2 "Yes, no problem if you exclude air (which goes without saying), and 7 "Hmmm, maybe, I dunno, good luck with that".
Mahalo, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 23 -- From tina-at-pbrc.hawaii.edu Wed May 13 13:38:59 2015 4, 23 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4DIcw9l010553 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 13:38:59 -0500 4, 23 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 23 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id t4DIcxBa023739 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 08:38:59 -1000 (HST) 4, 23 -- Received: from localhost (tina-at-localhost) 4, 23 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id t4DIcwUw023735 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 08:38:58 -1000 (HST) 4, 23 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 23 -- Date: Wed, 13 May 2015 08:38:58 -1000 (HST) 4, 23 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 23 -- X-X-Sender: tina-at-b1000 4, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 23 -- Subject: Re: [Microscopy] Can LR White be polymerized in plastic Petri dishes? 4, 23 -- In-Reply-To: {Pine.GSO.4.64.1505121522040.7442-at-b1000} 4, 23 -- Message-ID: {Pine.GSO.4.64.1505130834040.23506-at-b1000} 4, 23 -- References: {201505130115.t4D1FjrC027282-at-ns.microscopy.com} 4, 23 -- {3AE94C60-0817-4C92-9725-63C18F238E12-at-illinois.edu} {Pine.GSO.4.64.1505121522040.7442-at-b1000} 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
What is the best imaging mode\setup to image polymer blends samples?
Actually, I am trying to image a sample that contains PTB7:PC71BM with DIO. basically what I am looking for is to generate some contrast between these three materials. The first one is a sulfur-rich while the second rich with carbon.DIO is Iodine rich.
We tried to use HAADF and dark field imaging in STEM with low convergence angle probe in the objective off mode. Unfortunately, it was not successful.
We are not keen to attempt anything with EFTEM, as the doses required to get good signal to noise are rather high and likely to fry the sample. Additionally, finding small amounts of I using EFTEM will be very hard (slow rising edge at a fairly high energy of 619 eV gives very weak signal and therefore requires a huge dose to see it, which probably destroys the sample).
I would be very grateful for your help
Regards --
Ala' Afeef,
PhD Scholar - Glasgow University
==============================Original Headers============================== 10, 26 -- From alaa.afeef-at-gmail.com Wed May 13 15:35:20 2015 10, 26 -- Received: from mail-la0-f41.google.com (mail-la0-f41.google.com [209.85.215.41]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4DKZKRD003900 10, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 15:35:20 -0500 10, 26 -- Received: by layy10 with SMTP id y10so39821487lay.0 10, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 13:35:19 -0700 (PDT) 10, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 26 -- d=gmail.com; s=20120113; 10, 26 -- h=mime-version:from:date:message-id:subject:to:content-type; 10, 26 -- bh=J1p24MH993ezEGpXIy27L+itx2u7SHe3ptwfhdcCEzU=; 10, 26 -- b=Tb/1eTGSDyl7RZF2TiJmeD2zuCUgVu54epByf1HF0uXm9De/wjLF4o1NIk9ARDzHlA 10, 26 -- L2H4qA2kVeb2T6VGWSL2FE2Wgl/zxq5Um8Qv75SzeNcB4PzenltpnSHWmv02w+WSENAi 10, 26 -- fE6+RJmnhHAm+X2+cBwh+g+Ipn4159Vwu/tSvuJUqTg37kOwewP4CayYbv2A9jdvoA6f 10, 26 -- HBbnPd2BxZbXw+eVHyawuI9dQtDIfT9DcTe0R5iHI2qt7KVcklU0GUlKVCv/D2cmb+7o 10, 26 -- ViPvm8f9nBN89HWOCE3DEuU3DoMmnS0+vBFwOZaJn/D4HQD2Z0SCQ6vK1CZILepW2i3G 10, 26 -- TsVg== 10, 26 -- X-Received: by 10.112.189.102 with SMTP id gh6mr485665lbc.115.1431549319719; 10, 26 -- Wed, 13 May 2015 13:35:19 -0700 (PDT) 10, 26 -- MIME-Version: 1.0 10, 26 -- Received: by 10.114.77.5 with HTTP; Wed, 13 May 2015 13:34:59 -0700 (PDT) 10, 26 -- From: alaa afeef {alaa.afeef-at-gmail.com} 10, 26 -- Date: Wed, 13 May 2015 21:34:59 +0100 10, 26 -- Message-ID: {CAKsTHA22pt0hHU8Gpsz_0-iDeeVnTSmougmG35CoH_0Pz5pb9w-at-mail.gmail.com} 10, 26 -- Subject: STEM of polymer blends samples 10, 26 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" {Microscopy-at-microscopy.com} 10, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
If all you want is contrast then plasmonic imaging in EELS will be much, much lower dose than core loss EELS. Hereâs an example of plasmonic EELS imaging of block copolymers which will give you some idea of what can be done this way.
On May 13, 2015, at 1:54 PM, alaa.afeef-at-gmail.com wrote:
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Dear Listers,
May I have your help with the below issue:
What is the best imaging mode\setup to image polymer blends samples?
Actually, I am trying to image a sample that contains PTB7:PC71BM with DIO. basically what I am looking for is to generate some contrast between these three materials. The first one is a sulfur-rich while the second rich with carbon.DIO is Iodine rich.
We tried to use HAADF and dark field imaging in STEM with low convergence angle probe in the objective off mode. Unfortunately, it was not successful.
We are not keen to attempt anything with EFTEM, as the doses required to get good signal to noise are rather high and likely to fry the sample. Additionally, finding small amounts of I using EFTEM will be very hard (slow rising edge at a fairly high energy of 619 eV gives very weak signal and therefore requires a huge dose to see it, which probably destroys the sample).
I would be very grateful for your help
Regards --
Ala' Afeef,
PhD Scholar - Glasgow University
==============================Original Headers============================== 10, 26 -- From alaa.afeef-at-gmail.com Wed May 13 15:35:20 2015 10, 26 -- Received: from mail-la0-f41.google.com (mail-la0-f41.google.com [209.85.215.41]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4DKZKRD003900 10, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 15:35:20 -0500 10, 26 -- Received: by layy10 with SMTP id y10so39821487lay.0 10, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 13:35:19 -0700 (PDT) 10, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 26 -- d=gmail.com; s=20120113; 10, 26 -- h=mime-version:from:date:message-id:subject:to:content-type; 10, 26 -- bh=J1p24MH993ezEGpXIy27L+itx2u7SHe3ptwfhdcCEzU=; 10, 26 -- b=Tb/1eTGSDyl7RZF2TiJmeD2zuCUgVu54epByf1HF0uXm9De/wjLF4o1NIk9ARDzHlA 10, 26 -- L2H4qA2kVeb2T6VGWSL2FE2Wgl/zxq5Um8Qv75SzeNcB4PzenltpnSHWmv02w+WSENAi 10, 26 -- fE6+RJmnhHAm+X2+cBwh+g+Ipn4159Vwu/tSvuJUqTg37kOwewP4CayYbv2A9jdvoA6f 10, 26 -- HBbnPd2BxZbXw+eVHyawuI9dQtDIfT9DcTe0R5iHI2qt7KVcklU0GUlKVCv/D2cmb+7o 10, 26 -- ViPvm8f9nBN89HWOCE3DEuU3DoMmnS0+vBFwOZaJn/D4HQD2Z0SCQ6vK1CZILepW2i3G 10, 26 -- TsVg== 10, 26 -- X-Received: by 10.112.189.102 with SMTP id gh6mr485665lbc.115.1431549319719; 10, 26 -- Wed, 13 May 2015 13:35:19 -0700 (PDT) 10, 26 -- MIME-Version: 1.0 10, 26 -- Received: by 10.114.77.5 with HTTP; Wed, 13 May 2015 13:34:59 -0700 (PDT) 10, 26 -- From: alaa afeef {alaa.afeef-at-gmail.com} 10, 26 -- Date: Wed, 13 May 2015 21:34:59 +0100 10, 26 -- Message-ID: {CAKsTHA22pt0hHU8Gpsz_0-iDeeVnTSmougmG35CoH_0Pz5pb9w-at-mail.gmail.com} 10, 26 -- Subject: STEM of polymer blends samples 10, 26 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" {Microscopy-at-microscopy.com} 10, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 36 -- From zackg-at-berkeley.edu Wed May 13 16:07:24 2015 21, 36 -- Received: from mail-ig0-f172.google.com (mail-ig0-f172.google.com [209.85.213.172]) 21, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4DL7OnW025561 21, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 16:07:24 -0500 21, 36 -- Received: by igbsb11 with SMTP id sb11so56671612igb.0 21, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2015 14:07:23 -0700 (PDT) 21, 36 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 21, 36 -- d=1e100.net; s=20130820; 21, 36 -- h=x-gm-message-state:from:content-type:content-transfer-encoding 21, 36 -- :subject:date:references:to:message-id:mime-version; 21, 36 -- bh=sQsjVtmPMYZiLkSpBRr4fK6P5xTg3ZnRW/SKzSV8nbw=; 21, 36 -- b=D7Fzg/8Hu4CkGFMBkNvkIA4ysY4VGIURzmpZAAJ4J84Nv+QDt7gAg/wOLd37A3LAqX 21, 36 -- ytdH1Hrla38UyVBTlgpVM7uGpO8ejoOeLly4F77ttYsAQtuU2UAH7OX99ZrvGR3u/cEe 21, 36 -- 9cpG+kY8kwnHUOHe0JeIYNOQbw8BNUiuP08EBsKYvFDnGXRdpImoKu2TNMkj2+CY+wKn 21, 36 -- woVdj1qV3WGgUuRsgMsziYpG66B+skwdS3PCGhzsUJOPYZf5UbclaF3AOmtvvr/Z1cAJ 21, 36 -- CN4hfb0ZRjfIFuk+yKoQEMNE6NTiLfEPLrq3cFZYVVvfOCN9A1xUEuN83xYgyH1eG+Y6 21, 36 -- tqtA== 21, 36 -- X-Gm-Message-State: ALoCoQniN5MM7Jw8rcpMuRSvlzozE8/OpISPqoFrGeHUMrLQgq8Tt4k/CKPGwOCnwdsxBdPmMQWY 21, 36 -- X-Received: by 10.50.43.169 with SMTP id x9mr13311891igl.7.1431551243736; 21, 36 -- Wed, 13 May 2015 14:07:23 -0700 (PDT) 21, 36 -- Received: from new-host-2.home (pool-108-23-67-146.lsanca.fios.verizon.net. [108.23.67.146]) 21, 36 -- by mx.google.com with ESMTPSA id 7sm15055113iol.43.2015.05.13.14.07.22 21, 36 -- for {Microscopy-at-microscopy.com} 21, 36 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 21, 36 -- Wed, 13 May 2015 14:07:23 -0700 (PDT) 21, 36 -- From: Zack Gainsforth {zackg-at-berkeley.edu} 21, 36 -- Content-Type: text/plain; charset=utf-8 21, 36 -- Subject: Fwd: [Microscopy] STEM of polymer blends samples 21, 36 -- Date: Wed, 13 May 2015 14:07:22 -0700 21, 36 -- References: {7B344262-4574-4877-B937-89189BC2791F-at-berkeley.edu} 21, 36 -- To: Microscopy-at-microscopy.com 21, 36 -- Message-Id: {397D5D56-B231-4AFC-ADC0-7EBE313779AB-at-berkeley.edu} 21, 36 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2098\)) 21, 36 -- X-Mailer: Apple Mail (2.2098) 21, 36 -- Content-Transfer-Encoding: 8bit 21, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4DL7OnW025561 ==============================End of - Headers==============================
Hi all, My department EM lab is being dismantled. Our TEM is for sale. Let me know if you'd like the link to the listing site to bid on it. It is fully functional and in excellent condition but they do not list it that way for some odd reason. Good scope but film camera. Going cheap! It might be the last functional 902 in the US.
Sad time...but time to let it go. Beth
==============================Original Headers============================== 2, 41 -- From bethrichardson-at-uga.edu Thu May 14 16:38:45 2015 2, 41 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1on0135.outbound.protection.outlook.com [157.56.110.135]) 2, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4ELcj4f026142 2, 41 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2015 16:38:45 -0500 2, 41 -- Received: from BLUPR02MB212.namprd02.prod.outlook.com (10.242.189.151) by 2, 41 -- BLUPR02MB209.namprd02.prod.outlook.com (10.242.189.140) with Microsoft SMTP 2, 41 -- Server (TLS) id 15.1.160.19; Thu, 14 May 2015 21:38:44 +0000 2, 41 -- Received: from BLUPR02MB212.namprd02.prod.outlook.com ([169.254.8.47]) by 2, 41 -- BLUPR02MB212.namprd02.prod.outlook.com ([169.254.8.47]) with mapi id 2, 41 -- 15.01.0160.009; Thu, 14 May 2015 21:38:44 +0000 2, 41 -- From: Beth Richardson {bethrichardson-at-uga.edu} 2, 41 -- To: microscopy microscopy {microscopy-at-microscopy.com} 2, 41 -- Subject: Zeiss 902A TEM 2, 41 -- Thread-Topic: Zeiss 902A TEM 2, 41 -- Thread-Index: AQHQjo5ZbDlzBC802UG6tSXNbZw57Q== 2, 41 -- Date: Thu, 14 May 2015 21:38:43 +0000 2, 41 -- Message-ID: {9041B9BA-1966-448D-8A20-C3979987B96C-at-uga.edu} 2, 41 -- Accept-Language: en-US 2, 41 -- Content-Language: en-US 2, 41 -- X-MS-Has-Attach: 2, 41 -- X-MS-TNEF-Correlator: 2, 41 -- authentication-results: microscopy.com; dkim=none (message not signed) 2, 41 -- header.d=none; 2, 41 -- x-originating-ip: [198.137.20.110] 2, 41 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BLUPR02MB209; 2, 41 -- x-microsoft-antispam-prvs: {BLUPR02MB209776815D941AACEEBDA95DDD80-at-BLUPR02MB209.namprd02.prod.outlook.com} 2, 41 -- x-exchange-antispam-report-test: UriScan:; 2, 41 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5005006)(3002001);SRVR:BLUPR02MB209;BCL:0;PCL:0;RULEID:;SRVR:BLUPR02MB209; 2, 41 -- x-forefront-prvs: 0576145E86 2, 41 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(53754006)(229853001)(2656002)(99286002)(88552001)(89122001)(82746002)(83716003)(90282001)(102836002)(33656002)(106116001)(107886002)(5001960100002)(2900100001)(110136002)(46102003)(66066001)(50986999)(54356999)(189998001)(75432002)(92566002)(122556002)(36756003)(62966003)(77156002)(40100003)(87936001)(86362001)(450100001)(104396002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR02MB209;H:BLUPR02MB212.namprd02.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 2, 41 -- Content-Type: text/plain; charset="us-ascii" 2, 41 -- Content-ID: {BF173E81BE2DF04EB7969B363BCFB65C-at-namprd02.prod.outlook.com} 2, 41 -- MIME-Version: 1.0 2, 41 -- X-OriginatorOrg: uga.edu 2, 41 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 14 May 2015 21:38:43.9115 2, 41 -- (UTC) 2, 41 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 2, 41 -- X-MS-Exchange-CrossTenant-id: a8216c1e-4d63-4352-8c3b-50fa1f1475b1 2, 41 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BLUPR02MB209 2, 41 -- Content-Transfer-Encoding: 8bit 2, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4ELcj4f026142 ==============================End of - Headers==============================
From news3409384uerui-at-gmail.com Sat May 16 21:30:24 2015 Return-Path: {news3409384uerui-at-gmail.com} Received: from gmail.com ([27.255.72.80]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t4H2UL3f027735 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 16 May 2015 21:30:23 -0500 Message-ID: {38D2A266.56606CDD-at-gmail.com}
Hi listers,
I would like to get some input on epoxy embedding of Drosophila fat bodies. I understand, technically it should be similar to embedding adipocytes (which I have never done either) - so I expect problem with penetration during fixation. Any recommendations?
Thanks,
Michal Jarnik
==============================Original Headers============================== 5, 29 -- From michal.jarnik-at-nih.gov Sun May 17 04:54:09 2015 5, 29 -- Received: from nihxwayout.hub.nih.gov (nihxwayout.hub.nih.gov [128.231.90.109]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4H9s9mr016002 5, 29 -- for {microscopy-at-microscopy.com} ; Sun, 17 May 2015 04:54:09 -0500 5, 29 -- X-SBRS-Extended: Low 5, 29 -- X-IronPortListener: Outbound_SMTP 5, 29 -- X-IronPort-Anti-Spam-Filtered: true 5, 29 -- X-IronPort-Anti-Spam-Result: A2EJBQAIZVhV/4RHKJxcgxCBOMQDb4dFAQEIAoEZOhIBAQEBAQEBA4EHhCQBBBJ5ARUVQxMmAQQbGogKo0qFLa5gkA6DT4EWBYtxhnQHinOIAY8LI4N4gjSBAQEBAQ 5, 29 -- X-IPAS-Result: A2EJBQAIZVhV/4RHKJxcgxCBOMQDb4dFAQEIAoEZOhIBAQEBAQEBA4EHhCQBBBJ5ARUVQxMmAQQbGogKo0qFLa5gkA6DT4EWBYtxhnQHinOIAY8LI4N4gjSBAQEBAQ 5, 29 -- Received: from unknown (HELO msgb08.nih.gov) ([156.40.71.132]) 5, 29 -- by nihxwayout.hub.nih.gov with ESMTP/TLS/AES128-SHA; 17 May 2015 05:53:44 -0400 5, 29 -- Received: from MSGB03.nih.gov ([169.254.3.75]) by msgb08.nih.gov 5, 29 -- ([169.254.8.15]) with mapi id 14.03.0235.001; Sun, 17 May 2015 05:53:44 -0400 5, 29 -- From: "Jarnik, Michal (NIH/NICHD) [E]" {michal.jarnik-at-nih.gov} 5, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 29 -- Subject: Embedding fat tissue 5, 29 -- Thread-Topic: Embedding fat tissue 5, 29 -- Thread-Index: AdCQh1wytiv8XutkTHyBqOQVycu/zg== 5, 29 -- Date: Sun, 17 May 2015 09:53:43 +0000 5, 29 -- Message-ID: {A569C0CB3AAF894C9EA691CA2B2370831D451274-at-msgb03.nih.gov} 5, 29 -- Accept-Language: en-US 5, 29 -- Content-Language: en-US 5, 29 -- X-MS-Has-Attach: 5, 29 -- X-MS-TNEF-Correlator: 5, 29 -- x-originating-ip: [156.40.70.6] 5, 29 -- Content-Type: text/plain; charset="iso-8859-1" 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Transfer-Encoding: 8bit 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4H9s9mr016002 ==============================End of - Headers==============================
yet again a new chapter in the life of a biologist responsible for a FEI Tecnai G20! Our TEM is connected to a N2 cylinder to deliver the pressure necessary for the valves to work (full story at the end *). Now we got a new air compressor with all the necessary filters to avoid having water in the lines so I want to use it to feed the TEM valves with pressurized air. In the water/air rack, we have 2 manometers. The first one limits the pressure to 5 bar, the lines are labeled CCD camera, blow off (whatever it is) and probably the cushioning of the microscope (it is labeled "S34"). The second one is connected to the first one and limits the pressure to 0,3 bar, it is used for the TEM Valves.
I am concerned with the use of pressurized air in state of N2 because the second manometer has a label "N2 gas". So my questions:
- can I safely connect pressurized air in state of N2? - Is there any reason why I would absolutely need a N2 line? (for flushing, f.ex. during the procedure of SF6 filling, we bring a cylinder next to the microscope)
Many thanks in advance!
Stephane
* Full story here:
We then decided to use dry N2 from cylinders to feed the valves until we got a bug in the system, freezing not only the software but also one valve which stays open, wasting all the N2 in one night. Actually the bug does not occur very often and could be manageable if we woudn't have to order a new N2 cylinder each time. In other words, we are not ready (yet) to upgrade the PC and software just to solve this bug.
==============================Original Headers============================== 8, 34 -- From nizets2-at-yahoo.com Tue May 19 09:42:58 2015 8, 34 -- Received: from nm42-vm6.bullet.mail.ne1.yahoo.com (nm42-vm6.bullet.mail.ne1.yahoo.com [98.138.121.62]) 8, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4JEgvT2022342 8, 34 -- for {microscopy-at-microscopy.com} ; Tue, 19 May 2015 09:42:57 -0500 8, 34 -- Received: from [127.0.0.1] by nm42.bullet.mail.ne1.yahoo.com with NNFMP; 19 May 2015 14:42:57 -0000 8, 34 -- Received: from [98.138.100.117] by nm42.bullet.mail.ne1.yahoo.com with NNFMP; 19 May 2015 14:40:13 -0000 8, 34 -- Received: from [66.196.81.172] by tm108.bullet.mail.ne1.yahoo.com with NNFMP; 19 May 2015 14:40:13 -0000 8, 34 -- Received: from [98.139.212.211] by tm18.bullet.mail.bf1.yahoo.com with NNFMP; 19 May 2015 14:40:13 -0000 8, 34 -- Received: from [127.0.0.1] by omp1020.mail.bf1.yahoo.com with NNFMP; 19 May 2015 14:40:13 -0000 8, 34 -- X-Yahoo-Newman-Property: ymail-4 8, 34 -- X-Yahoo-Newman-Id: 672277.95673.bm-at-omp1020.mail.bf1.yahoo.com 8, 34 -- Received: (qmail 88052 invoked by uid 60001); 19 May 2015 14:40:13 -0000 8, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1432046413; bh=xblQgxBzYew4/8cc/CmcGffsiiRKbD55ebw/sFpgchI=; h=Message-ID:Date:From:Subject:To:MIME-Version:Content-Type; b=Ornmf7KR20Y0A2QpijNj/3ygw9MYHHceX2b0jdBx8cRBJD6JiSTCk83rCq58y/6XzGrKtY9QBH5az1rOqschaukBod9/BIVUop5UDcf/9M2GomgH0mVaj4bS1uQguRcRy9WZMhXuYuxFp5mkfDox4/qxIYQ4skI/3o4t25k15L0= 8, 34 -- X-YMail-OSG: E44DEpcVM1kSmoju4d87vB6ROPyYImXCG9kBAfjWf49R4M3 8, 34 -- 1Y0N5ZfoeNk5ZLCEHRnanRP3UXpxRdpmqjhUcdoEel7Sd8J7jAqUAfpXOUEe 8, 34 -- c2psnRUvgVTUimsfqYNDh9ZmaqE9EKDoyxczqIyBNMLfgVEOabPgVGIv3l9t 8, 34 -- DgSgvamjW1xOKBBLX59hX0FiwBG_aQ4tloOgoI42N5djh.cIxZj7ktqHbmtD 8, 34 -- 1r56WVMsGaEahoEW7QckjxRHhnVJIZ7jYLMD6EWMHyl.6ygujTLUpNxEgoNg 8, 34 -- KjTZ4YtaGJTaSPmg7PR_fDpudtBdjrx7Vz4tPIRkS9z8Bg1JTWRexnFfjI91 8, 34 -- taVnWcpuhkQWO4MduLNK3Xjk8bWKM91gaZioanNQpcmXfL0A6jPfboircwLh 8, 34 -- 4EDSU1.DxywYTIU5sAlW2hfVAi4myN8Ou8EsjBYkkGdXOZehQfGUuVovVlfT 8, 34 -- PfanGxUphbeTPD1Edzi4LOddP2oq7nLM.hTblSgBZ7b7W88AIes9cqY9ziZX 8, 34 -- 87U6FUJs0mBHhoQzcK6zGfJk6_CgTihRHwv871Y2erQKhfZ6SKiyOyjeHhzq 8, 34 -- mR8nbYeEsBLWISI4- 8, 34 -- Received: from [93.83.199.146] by web141702.mail.bf1.yahoo.com via HTTP; Tue, 19 May 2015 07:40:13 PDT 8, 34 -- X-Rocket-MIMEInfo: 002.001,RGVhciBjb2xsZWFndWVzLA0KDQp5ZXQgYWdhaW4gYSBuZXcgY2hhcHRlciBpbiB0aGUgbGlmZSBvZiBhIGJpb2xvZ2lzdCByZXNwb25zaWJsZSBmb3IgYSBGRUkgVGVjbmFpIEcyMCENCk91ciBURU0gaXMgY29ubmVjdGVkIHRvIGEgTjIgY3lsaW5kZXIgdG8gZGVsaXZlciB0aGUgcHJlc3N1cmUgbmVjZXNzYXJ5IGZvciB0aGUgdmFsdmVzIHRvIHdvcmsgKGZ1bGwgc3RvcnkgYXQgdGhlIGVuZCAqKS4NCk5vdyB3ZSBnb3QgYSBuZXcgYWlyIGNvbXByZXNzb3Igd2l0aCBhbGwgdGhlIG5lY2Vzc2FyeSBmaWx0ZXIBMAEBAQE- 8, 34 -- X-Mailer: YahooMailClassic/519 YahooMailWebService/0.8.203.740 8, 34 -- Message-ID: {1432046413.44324.YahooMailBasic-at-web141702.mail.bf1.yahoo.com} 8, 34 -- Date: Tue, 19 May 2015 07:40:13 -0700 8, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 8, 34 -- Subject: Pressurized air vs N2 8, 34 -- To: microscopy-at-microscopy.com 8, 34 -- MIME-Version: 1.0 8, 34 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
You can use pressurized air as your main air supply. The N2 line is for back filling as in a filament exchange, column vent or camera vent. Bringing in a gas cylinder when needed works well.
John
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, May 19, 2015 11:03 AM To: John J. Schreiber
Dear colleagues,
yet again a new chapter in the life of a biologist responsible for a FEI Tecnai G20! Our TEM is connected to a N2 cylinder to deliver the pressure necessary for the valves to work (full story at the end *). Now we got a new air compressor with all the necessary filters to avoid having water in the lines so I want to use it to feed the TEM valves with pressurized air. In the water/air rack, we have 2 manometers. The first one limits the pressure to 5 bar, the lines are labeled CCD camera, blow off (whatever it is) and probably the cushioning of the microscope (it is labeled "S34"). The second one is connected to the first one and limits the pressure to 0,3 bar, it is used for the TEM Valves.
I am concerned with the use of pressurized air in state of N2 because the second manometer has a label "N2 gas". So my questions:
- can I safely connect pressurized air in state of N2? - Is there any reason why I would absolutely need a N2 line? (for flushing, f.ex. during the procedure of SF6 filling, we bring a cylinder next to the microscope)
Many thanks in advance!
Stephane
* Full story here:
We then decided to use dry N2 from cylinders to feed the valves until we got a bug in the system, freezing not only the software but also one valve which stays open, wasting all the N2 in one night. Actually the bug does not occur very often and could be manageable if we woudn't have to order a new N2 cylinder each time. In other words, we are not ready (yet) to upgrade the PC and software just to solve this bug.
==============================Original Headers============================== 8, 34 -- From nizets2-at-yahoo.com Tue May 19 09:42:58 2015 8, 34 -- Received: from nm42-vm6.bullet.mail.ne1.yahoo.com (nm42-vm6.bullet.mail.ne1.yahoo.com [98.138.121.62]) 8, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4JEgvT2022342 8, 34 -- for {microscopy-at-microscopy.com} ; Tue, 19 May 2015 09:42:57 -0500 8, 34 -- Received: from [127.0.0.1] by nm42.bullet.mail.ne1.yahoo.com with NNFMP; 19 May 2015 14:42:57 -0000 8, 34 -- Received: from [98.138.100.117] by nm42.bullet.mail.ne1.yahoo.com with NNFMP; 19 May 2015 14:40:13 -0000 8, 34 -- Received: from [66.196.81.172] by tm108.bullet.mail.ne1.yahoo.com with NNFMP; 19 May 2015 14:40:13 -0000 8, 34 -- Received: from [98.139.212.211] by tm18.bullet.mail.bf1.yahoo.com with NNFMP; 19 May 2015 14:40:13 -0000 8, 34 -- Received: from [127.0.0.1] by omp1020.mail.bf1.yahoo.com with NNFMP; 19 May 2015 14:40:13 -0000 8, 34 -- X-Yahoo-Newman-Property: ymail-4 8, 34 -- X-Yahoo-Newman-Id: 672277.95673.bm-at-omp1020.mail.bf1.yahoo.com 8, 34 -- Received: (qmail 88052 invoked by uid 60001); 19 May 2015 14:40:13 -0000 8, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1432046413; bh=xblQgxBzYew4/8cc/CmcGffsiiRKbD55ebw/sFpgchI=; h=Message-ID:Date:From:Subject:To:MIME-Version:Content-Type; b=Ornmf7KR20Y0A2QpijNj/3ygw9MYHHceX2b0jdBx8cRBJD6JiSTCk83rCq58y/6XzGrKtY9QBH5az1rOqschaukBod9/BIVUop5UDcf/9M2GomgH0mVaj4bS1uQguRcRy9WZMhXuYuxFp5mkfDox4/qxIYQ4skI/3o4t25k15L0= 8, 34 -- X-YMail-OSG: E44DEpcVM1kSmoju4d87vB6ROPyYImXCG9kBAfjWf49R4M3 8, 34 -- 1Y0N5ZfoeNk5ZLCEHRnanRP3UXpxRdpmqjhUcdoEel7Sd8J7jAqUAfpXOUEe 8, 34 -- c2psnRUvgVTUimsfqYNDh9ZmaqE9EKDoyxczqIyBNMLfgVEOabPgVGIv3l9t 8, 34 -- DgSgvamjW1xOKBBLX59hX0FiwBG_aQ4tloOgoI42N5djh.cIxZj7ktqHbmtD 8, 34 -- 1r56WVMsGaEahoEW7QckjxRHhnVJIZ7jYLMD6EWMHyl.6ygujTLUpNxEgoNg 8, 34 -- KjTZ4YtaGJTaSPmg7PR_fDpudtBdjrx7Vz4tPIRkS9z8Bg1JTWRexnFfjI91 8, 34 -- taVnWcpuhkQWO4MduLNK3Xjk8bWKM91gaZioanNQpcmXfL0A6jPfboircwLh 8, 34 -- 4EDSU1.DxywYTIU5sAlW2hfVAi4myN8Ou8EsjBYkkGdXOZehQfGUuVovVlfT 8, 34 -- PfanGxUphbeTPD1Edzi4LOddP2oq7nLM.hTblSgBZ7b7W88AIes9cqY9ziZX 8, 34 -- 87U6FUJs0mBHhoQzcK6zGfJk6_CgTihRHwv871Y2erQKhfZ6SKiyOyjeHhzq 8, 34 -- mR8nbYeEsBLWISI4- 8, 34 -- Received: from [93.83.199.146] by web141702.mail.bf1.yahoo.com via HTTP; Tue, 19 May 2015 07:40:13 PDT 8, 34 -- X-Rocket-MIMEInfo: 002.001,RGVhciBjb2xsZWFndWVzLA0KDQp5ZXQgYWdhaW4gYSBuZXcgY2hhcHRlciBpbiB0aGUgbGlmZSBvZiBhIGJpb2xvZ2lzdCByZXNwb25zaWJsZSBmb3IgYSBGRUkgVGVjbmFpIEcyMCENCk91ciBURU0gaXMgY29ubmVjdGVkIHRvIGEgTjIgY3lsaW5kZXIgdG8gZGVsaXZlciB0aGUgcHJlc3N1cmUgbmVjZXNzYXJ5IGZvciB0aGUgdmFsdmVzIHRvIHdvcmsgKGZ1bGwgc3RvcnkgYXQgdGhlIGVuZCAqKS4NCk5vdyB3ZSBnb3QgYSBuZXcgYWlyIGNvbXByZXNzb3Igd2l0aCBhbGwgdGhlIG5lY2Vzc2FyeSBmaWx0ZXIBMAEBAQE- 8, 34 -- X-Mailer: YahooMailClassic/519 YahooMailWebService/0.8.203.740 8, 34 -- Message-ID: {1432046413.44324.YahooMailBasic-at-web141702.mail.bf1.yahoo.com} 8, 34 -- Date: Tue, 19 May 2015 07:40:13 -0700 8, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 8, 34 -- Subject: Pressurized air vs N2 8, 34 -- To: microscopy-at-microscopy.com 8, 34 -- MIME-Version: 1.0 8, 34 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
==============================Original Headers============================== 17, 39 -- From js51-at-princeton.edu Tue May 19 10:13:41 2015 17, 39 -- Received: from Princeton.EDU (ppa03.Princeton.EDU [128.112.128.214]) 17, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4JFDew1010785 17, 39 -- for {Microscopy-at-microscopy.com} ; Tue, 19 May 2015 10:13:40 -0500 17, 39 -- Received: from csgsmtp200l.Princeton.EDU (csgsmtp200l.Princeton.EDU [128.112.130.131]) 17, 39 -- by ppa03.princeton.edu (8.14.5/8.14.5) with ESMTP id t4JFDclm006340 17, 39 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 17, 39 -- for {Microscopy-at-microscopy.com} ; Tue, 19 May 2015 11:13:39 -0400 17, 39 -- Received: from CSGHUB208W.pu.win.princeton.edu (csghub208w.Princeton.EDU [10.6.60.210]) 17, 39 -- by csgsmtp200l.Princeton.EDU (8.13.8/8.12.9) with ESMTP id t4JFDW2Q007853; 17, 39 -- Tue, 19 May 2015 11:13:38 -0400 17, 39 -- Received: from CSGMBX205W.pu.win.princeton.edu ([169.254.6.186]) by 17, 39 -- CSGHUB208W.pu.win.princeton.edu ([10.6.60.210]) with mapi id 14.03.0235.001; 17, 39 -- Tue, 19 May 2015 11:13:14 -0400 17, 39 -- From: "John J. Schreiber" {js51-at-princeton.edu} 17, 39 -- To: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} 17, 39 -- CC: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 17, 39 -- Subject: RE: [Microscopy] Pressurized air vs N2 17, 39 -- Thread-Topic: [Microscopy] Pressurized air vs N2 17, 39 -- Thread-Index: AQHQkkTm+/99Xh/AvE2LeLp6m2oGnp2DZwZg 17, 39 -- Date: Tue, 19 May 2015 15:13:13 +0000 17, 39 -- Message-ID: {3C628C43B600C14EB700249D43D718D312645248-at-CSGMBX205W.pu.win.princeton.edu} 17, 39 -- References: {201505191503.t4JF3Na1008495-at-ns.microscopy.com} 17, 39 -- In-Reply-To: {201505191503.t4JF3Na1008495-at-ns.microscopy.com} 17, 39 -- Accept-Language: en-US 17, 39 -- Content-Language: en-US 17, 39 -- X-MS-Has-Attach: 17, 39 -- X-MS-TNEF-Correlator: 17, 39 -- x-originating-ip: [128.112.141.39] 17, 39 -- Content-Type: text/plain; charset="us-ascii" 17, 39 -- MIME-Version: 1.0 17, 39 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.14.151,1.0.33,0.0.0000 17, 39 -- definitions=2015-05-19_05:2015-05-19,2015-05-19,1970-01-01 signatures=0 17, 39 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 17, 39 -- suspectscore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 17, 39 -- adjust=0 reason=mlx scancount=1 engine=7.0.1-1402240000 17, 39 -- definitions=main-1505190191 17, 39 -- Content-Transfer-Encoding: 8bit 17, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4JFDew1010785 ==============================End of - Headers==============================
Like The Venerable Bicycle, ItĄŚs Safe As Directed! Because of the scientific appeal as well as the economic benefits associated with discovery, numerous attempts have been made over the past decade to find a substance equal to or better than Uranyl Acetate for medical testing and scientific research. The results of these experiments are in: there is no effective replacement for Uranyl Acetate. At IBI Labs, we think the use of Uranyl Acetate is analogous to the bicycle. Dating to the early 1800s according to historical records, the bicycle ĄV while enhanced with bells and accessories ĄV today remains a simple and effective method of personal conveyance. The bicycle really has not changed since invention. Improvements have been made but still it is a combination of wheels, seat, handlebars, sprockets, chain, and pedals. And while one can engage in risky behavior with just about any device, a bicycle is safe as directed. TodayĄŚs guidelines for use are quite straightforward: watch where youĄŚre going and wear a helmet! And so it is with Uranyl Acetate. Follow generally accepted scientific practices ĄV also known as reading the directions -- and wear laboratory-grade rubber gloves. ItĄŚs that basic. Simply, the extensive and longstanding use of Uranyl Acetate as a staining method in electron microscopy can be traced to the following key attributes: Ď Uranyl Acetate staining is easy and quick to perform,
Ď Uranyl Acetate staining provides results within a few minutes,
Ď Uranyl Acetate emits a very low level of alpha radiation: 0.37ĄV0.51 microcuries (14ĄV19 kBq) per gram,
Ď Urany AcetateĄŚs alpha radiation is not harmful when the material remains external to the human body,
Ď Uranyl Acetate is commercially available, reasonably priced, and permitted for national and international shipping. Uranyl Acetate: Tried and True The analogy of Uranyl Acetate to the bicycle doesnĄŚt end with the above items. Extensive experience in the marketplace suggests that despite attempts by some outliers to introduce alternatives -- such as the highly toxic and only marginally effective IBI Labs Blue Platinum* -- scientific and medical researchers find using Uranyl Acetate a lot like riding a bike: once learned, itĄŚs never forgotten. Tried ĄK true ĄK reliable ĄK safe ĄK effective ĄK thatĄŚs Uranyl Acetate in a 50 ml beaker (the scientific equivalent of a nutshell)!
Jump On The Bandwagon Need a breath of fresh air and some exercise? Hop on a bike ĄK few activities rival the simple, venerable bicycle. Need a staining method for electron microscopy? Jump on the Uranyl Acetate bandwagon ĄK or if youĄŚve been on it, donĄŚt be tempted to get off! No other substance ĄV past or present ĄV rivals Uranyl Acetate for effectiveness and reliability.
For more information on Uranyl Acetate, go to ibilabs.com -----
*IBI Labs Blue Platinum has yielded positive reviews in limited, narrowly focused applications. To review the Technical Notes associated with these reviews, go to IBIlabs.com and click on protocols About IBI
International Bio-analytical Industries, Inc. (IBI) is the worldĄŚs leading manufacturer and distributor of specialty Uranium and Thorium compounds as well as related research and medical products. Founded in 2002, IBI specializes in production of Uranyl Acetate, Uranyl Nitrate, Uranyl Tetrafluoride, Uranyl Oxide, and other fine specialty Uranium compounds and reagents manufactured exclusively from depleted Uranium.
-- INTERNATIONAL BIO-ANALYTICAL INDUSTRIES,INC.
3495 N. Dixie Hwy. Unit # 8 Boca Raton, Florida 33431
Tel: 561-826-0061 Fax: 561-892-8450 Web Site: http://www.ibilabs.com Email: bioanalytics-at-ibilabs.com
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==============================Original Headers============================== 18, 46 -- From bioanalytics-at-ibilabs.com Wed May 20 08:43:40 2015 18, 46 -- Received: from linux36.domainnameservers.net (linux36.domainnameservers.net [173.193.173.82]) 18, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4KDhd00027459 18, 46 -- for {Microscopy-at-microscopy.com} ; Wed, 20 May 2015 08:43:40 -0500 18, 46 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; d=ibilabs.com; s=default; 18, 46 -- h=Content-Transfer-Encoding:Content-Type:MIME-Version:To:From:Subject:Date:Message-ID; bh=njQcVF9PPlK8c7LRj3duR5O2+oEF0XznNYymMmsp0iE=; 18, 46 -- b=EBeFk7XN2XhpRosyEZPDoRdTxTXzUV6B3sDPOSn8z4Bo66x0LanYwEwjSjvPVHokZ3AAwnhAjtBb3tD0N2Je7pb8f+0H1Ep/hTurIq+cmSO9f3VdG6cdO1XoTlVPl5ic1eYkRop0mAFfhxgNUKoCZS69oxCc3r6LFPoMFUW5HvQ=; 18, 46 -- Received: from localhost ([127.0.0.1]:41984 helo=linux36.domainnameservers.net) 18, 46 -- by linux36.domainnameservers.net with esmtpa (Exim 4.85) 18, 46 -- (envelope-from {bioanalytics-at-ibilabs.com} ) 18, 46 -- id 1Yv4HC-0000bL-DC 18, 46 -- for Microscopy-at-microscopy.com; Wed, 20 May 2015 09:43:14 -0400 18, 46 -- Received: from 99.181.22.54 ([99.181.22.54]) 18, 46 -- (SquirrelMail authenticated user bioanalytics-at-ibilabs.com) 18, 46 -- by linux36.domainnameservers.net with HTTP; 18, 46 -- Wed, 20 May 2015 09:43:14 -0400 18, 46 -- Message-ID: {c7b321d55cd0570cceac4d405c02a22d.squirrel-at-linux36.domainnameservers.net} 18, 46 -- Date: Wed, 20 May 2015 09:43:14 -0400 18, 46 -- Subject: No effective replacement for uranyl acetate 18, 46 -- From: "IBILABS" {bioanalytics-at-ibilabs.com} 18, 46 -- To: Microscopy-at-microscopy.com 18, 46 -- User-Agent: SquirrelMail/1.5.2 [SVN] 18, 46 -- MIME-Version: 1.0 18, 46 -- Content-Type: text/plain;charset=iso-8859-1 18, 46 -- Content-Transfer-Encoding: 8bit 18, 46 -- X-Confirm-Reading-To: "IBILABS" {bioanalytics-at-ibilabs.com} 18, 46 -- Disposition-Notification-To: "IBILABS" {bioanalytics-at-ibilabs.com} 18, 46 -- X-Priority: 1 (Highest) 18, 46 -- Importance: High 18, 46 -- X-Buzix-MailScanner-Information: Please contact the ISP for more information 18, 46 -- X-Buzix-MailScanner-ID: 1Yv4HC-0000bL-DC 18, 46 -- X-Buzix-MailScanner: Found to be clean 18, 46 -- X-Buzix-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 18, 46 -- score=-2.899, required 5, autolearn=not spam, ALL_TRUSTED -1.00, 18, 46 -- BAYES_00 -1.90, URIBL_BLOCKED 0.00) 18, 46 -- X-Buzix-MailScanner-From: bioanalytics-at-ibilabs.com 18, 46 -- X-Spam-Status: No 18, 46 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 18, 46 -- X-AntiAbuse: Primary Hostname - linux36.domainnameservers.net 18, 46 -- X-AntiAbuse: Original Domain - microscopy.com 18, 46 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 18, 46 -- X-AntiAbuse: Sender Address Domain - ibilabs.com 18, 46 -- X-Get-Message-Sender-Via: linux36.domainnameservers.net: authenticated_id: bioanalytics-at-ibilabs.com 18, 46 -- X-Source: 18, 46 -- X-Source-Args: 18, 46 -- X-Source-Dir: ==============================End of - Headers==============================
==============================Original Headers============================== 10, 24 -- From ian-at-acutance.co.uk Wed May 20 12:32:00 2015 10, 24 -- Received: from mailex.mailcore.me (mailex.mailcore.me [94.136.40.145]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4KHVxVP026367 10, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 20 May 2015 12:32:00 -0500 10, 24 -- Received: from [2.28.164.22] (helo=THINKdesktop13) 10, 24 -- by smtp04.mailcore.me with esmtpa (Exim 4.80.1) 10, 24 -- (envelope-from {ian-at-acutance.co.uk} ) 10, 24 -- id 1Yv7qZ-0006Rd-DK 10, 24 -- for Microscopy-at-microscopy.com; Wed, 20 May 2015 18:31:59 +0100 10, 24 -- From: "Ian Holton" {ian-at-acutance.co.uk} 10, 24 -- To: {Microscopy-at-microscopy.com} 10, 24 -- Subject: Does anyone have experience of SGX EDS? 10, 24 -- Date: Wed, 20 May 2015 18:31:56 +0100 10, 24 -- Message-ID: {019c01d09322$ded02b20$9c708160$-at-acutance.co.uk} 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; 10, 24 -- charset="iso-8859-1" 10, 24 -- X-Mailer: Microsoft Outlook 15.0 10, 24 -- Thread-Index: AdCTIsmOA90Qe004TGKfcM2sZP41Jw== 10, 24 -- Content-Language: en-gb 10, 24 -- X-Mailcore-Auth: 10108238 10, 24 -- X-Mailcore-Domain: 1117517 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4KHVxVP026367 ==============================End of - Headers==============================
From advertisebz09uiva-at-gmail.com Wed May 20 23:51:15 2015 Return-Path: {advertisebz09uiva-at-gmail.com} Received: from gmail.com ([1.234.25.151]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t4L4pCaV011348 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 20 May 2015 23:51:14 -0500 Message-ID: {7764FF5C.089D60DB-at-gmail.com}
SGX makes a REALLY nice smart phone app for looking up X-ray line energies!
John Mardinly, ASU
-----Original Message----- X-from: ian-at-acutance.co.uk [mailto:ian-at-acutance.co.uk] Sent: Wednesday, May 20, 2015 10:46 AM To: John Mardinly
Dear Listers
A friend asks whether I have experience with SGX Sensortech EDS - I don't, but I offered to ask the question on this site.
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I just read a post on uranyl acetate and wanted ti ask you a question regarding a particular uranyl acetate sold by EMS as non radioactive. Does this work same as radioactive ones? Has anybody used this non-radioactive uranyl acetate? Does it have any kind of limitations?
Their description is at this website: http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx#22400
Unicryl Embedding and Staining Kit
Please see listing in Embedding Media Kits. RT 14660 kit 300.00 Add to Cart msdsarrowUranyl Acetate, Reagent, A.C.S.
UO2(OCOCH3)2ˇ2H2O F.W. 424.14 CAS #541-09-3 Assay 98.0-102.0% Specifications: Insoluble Matters 0.01 Chloride 0.003% Sulfate 0.01% Alkalies (as SO4) 0.05% Heavy Metals 0.002% Iron 0.001%
A universal EM stain for thin sections, en-block staining, and negative staining. RT 22400 25g 155.00 Add to Cart
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==============================Original Headers============================== 20, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu May 21 19:38:04 2015 20, 28 -- Received: from znl.com ([206.69.208.20]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4M0c4J4013315 20, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2015 19:38:04 -0500 20, 28 -- Received: from localhost (localhost [127.0.0.1]) 20, 28 -- by znl.com (Postfix) with ESMTP id 6E69C4E9A23 20, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2015 19:38:04 -0500 (CDT) 20, 28 -- X-Virus-Scanned: amavisd-new at znl.com 20, 28 -- Received: from znl.com ([127.0.0.1]) 20, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 20, 28 -- with ESMTP id 8WUFyHBJkLhn for {microscopy-at-microscopy.com} ; 20, 28 -- Thu, 21 May 2015 19:37:50 -0500 (CDT) 20, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 20, 28 -- by znl.com (Postfix) with ESMTPA id 821154E9A0C 20, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2015 19:37:50 -0500 (CDT) 20, 28 -- Message-ID: {555E7A5E.6000400-at-microscopy.com} 20, 28 -- Date: Thu, 21 May 2015 19:37:50 -0500 20, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 20, 28 -- MIME-Version: 1.0 20, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 28 -- Subject: viaWWW:non-radioactive uranly acetate 20, 28 -- References: {201505210100.t4L10Ox6003046-at-ns.microscopy.com} 20, 28 -- In-Reply-To: {201505210100.t4L10Ox6003046-at-ns.microscopy.com} 20, 28 -- X-Forwarded-Message-Id: {201505210100.t4L10Ox6003046-at-ns.microscopy.com} 20, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Unknown structure in Reptile RBC.
Message: We got unusual structure in reptile RBC. Can any body tell me what is this ? What are the histological stains to identify this structure.?
Figure 2 High Power: https://www.flickr.com/photos/97321550-at-N08/17937041251/in/dateposted-public/
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==============================Original Headers============================== 10, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu May 21 19:38:46 2015 10, 28 -- Received: from znl.com ([206.69.208.20]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4M0ckAU013768 10, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2015 19:38:46 -0500 10, 28 -- Received: from localhost (localhost [127.0.0.1]) 10, 28 -- by znl.com (Postfix) with ESMTP id 155364E9A40 10, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2015 19:38:46 -0500 (CDT) 10, 28 -- X-Virus-Scanned: amavisd-new at znl.com 10, 28 -- Received: from znl.com ([127.0.0.1]) 10, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id XSndKP7ee-BI for {microscopy-at-microscopy.com} ; 10, 28 -- Thu, 21 May 2015 19:38:38 -0500 (CDT) 10, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 10, 28 -- by znl.com (Postfix) with ESMTPA id B99CA4E9A36 10, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2015 19:38:38 -0500 (CDT) 10, 28 -- Message-ID: {555E7A8E.1070303-at-microscopy.com} 10, 28 -- Date: Thu, 21 May 2015 19:38:38 -0500 10, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 10, 28 -- MIME-Version: 1.0 10, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 28 -- Subject: viaWWW:Unknown structure in Reptile RBC 10, 28 -- References: {201505211553.t4LFrkvL030359-at-ns.microscopy.com} 10, 28 -- In-Reply-To: {201505211553.t4LFrkvL030359-at-ns.microscopy.com} 10, 28 -- X-Forwarded-Message-Id: {201505211553.t4LFrkvL030359-at-ns.microscopy.com} 10, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Many thanks indeed for all the feedback. A pretty useful picture emerges from all this and I will feed it all back to my friend.
Many thanks again for your help.
Ian
-----Original Message----- X-from: John Mitchels [mailto:john.mitchels-at-gmail.com] Sent: 21 May 2015 22:10 To: ian-at-acutance.co.uk
Dear All, I was thinking of buying a portable Gauss meter last week to try and track down some electrical interference in our lab. Comment from a colleague - 'oh, just download a free app for your phone'. So I did; I think there are several ones available but I got Teslameter. It looks really good - and seems to work with reading levels down to 0.1mG. Does anyone know if these apps actually work as well they appear to? Are they accurate?
Richard
==============================Original Headers============================== 3, 32 -- From contact-at-integrityscientific.com Fri May 22 03:47:41 2015 3, 32 -- Received: from mo4-p01-ob.smtp.rzone.de (mo4-p01-ob.smtp.rzone.de [81.169.146.165]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4M8leIA032312 3, 32 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2015 03:47:41 -0500 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; t=1432284460; l=474; 3, 32 -- s=domk; d=integrityscientific.com; 3, 32 -- h=Mime-Version:Content-Transfer-Encoding:To:Date:Content-Type:From: 3, 32 -- Subject; 3, 32 -- bh=e/37PnhXyC7WX9UtDT8F/tqw9VzVM4IhCfqXI4rZ+Y8=; 3, 32 -- b=rT+wy8e8+XgZQ7jtRqN+bcQKbY4IZMXCfEKJhkuArlGDF8ynXewOgLTKY1FoPkp1wp+ 3, 32 -- f1yBDS9X1mrXLCFA8usC8l8/UWr3XDiugz3ihURDzQs6nSayhXMRHBrtSmAOW4UyeCgea 3, 32 -- MGr1Lc2lmAtFOx9R07zzgudp+/oJ45JssVg= 3, 32 -- X-RZG-AUTH: :L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBSdqsy65lHRHJMBLi7o7zMYgEOC6u2s= 3, 32 -- X-RZG-CLASS-ID: mo01 3, 32 -- Received: from [192.168.1.6] (host-2-97-120-126.as13285.net [2.97.120.126]) 3, 32 -- by smtp.strato.com (RZmta 37.6 DYNA|AUTH) 3, 32 -- with ESMTPSA id g03805r4M8ldg3u 3, 32 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (curve secp521r1 with 521 ECDH bits, eq. 15360 bits RSA)) 3, 32 -- (Client did not present a certificate) 3, 32 -- for {microscopy-at-microscopy.com} ; 3, 32 -- Fri, 22 May 2015 10:47:39 +0200 (CEST) 3, 32 -- Subject: Gauss meter app for smartphones 3, 32 -- From: Integrity Scientific Ltd {contact-at-integrityscientific.com} 3, 32 -- Content-Type: text/plain; 3, 32 -- charset=us-ascii 3, 32 -- X-Mailer: iPad Mail (12B435) 3, 32 -- Message-Id: {B61EBC4A-7964-4738-8DC5-32B59A2FD11E-at-integrityscientific.com} 3, 32 -- Date: Fri, 22 May 2015 09:47:42 +0100 3, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4M8leIA032312 ==============================End of - Headers==============================
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we are planing to get a high vacuum coater. We have two in our hands: a Leica ACE600 and a Quorum Q150R. Any inputs, e.g. complains, thoughts, opinions, would be greatly appreciated.
Best
Leandro
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri May 22 06:32:55 2015 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4MBWtJf027494 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2015 06:32:55 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 2D7734EF810 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2015 06:32:52 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at znl.com 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id VEAwzexNTus2 for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 22 May 2015 06:32:44 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 9FE414EF807 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2015 06:32:44 -0500 (CDT) 14, 28 -- Message-ID: {555F13DC.1080501-at-microscopy.com} 14, 28 -- Date: Fri, 22 May 2015 06:32:44 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:High vacuum coater 14, 28 -- References: {201505221058.t4MAw2Ps025775-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201505221058.t4MAw2Ps025775-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201505221058.t4MAw2Ps025775-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Greetings Richard, There are I think two dimensions to this question. The first is technical. If you are trying to track down sources of interference in your lab, you are probably concerned with time-varying magnetic fields - often at 60 Hz. The sensors in a phone will give DC readings, and thus they are not useful to you. I bought a Tenmars TM-192 3-axis EMF meter ( {$200), which I compared to a $2000 Hall effect probe, and I found it to compare nicely for purposes of walking around a space and seeing where "hot spots" are. The second dimension is economic. Do you want to base expensive decisions such as room design, instrument selection, instrument placement or interference abatement on an app you got for free? Be sure your investment in information gathering is in proportion to the commitments you make based on the information.
Regards, Larry Scipioni ZS Genetics
-----Original Message----- X-from: contact-at-integrityscientific.com [mailto:contact-at-integrityscientific.com] Sent: Friday, May 22, 2015 5:02 AM To: LES-at-ZSGENETICS.COM
Dear All, I was thinking of buying a portable Gauss meter last week to try and track down some electrical interference in our lab. Comment from a colleague - 'oh, just download a free app for your phone'. So I did; I think there are several ones available but I got Teslameter. It looks really good - and seems to work with reading levels down to 0.1mG. Does anyone know if these apps actually work as well they appear to? Are they accurate?
Richard
==============================Original Headers============================== 3, 32 -- From contact-at-integrityscientific.com Fri May 22 03:47:41 2015 3, 32 -- Received: from mo4-p01-ob.smtp.rzone.de (mo4-p01-ob.smtp.rzone.de [81.169.146.165]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4M8leIA032312 3, 32 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2015 03:47:41 -0500 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; t=1432284460; l=474; 3, 32 -- s=domk; d=integrityscientific.com; 3, 32 -- h=Mime-Version:Content-Transfer-Encoding:To:Date:Content-Type:From: 3, 32 -- Subject; 3, 32 -- bh=e/37PnhXyC7WX9UtDT8F/tqw9VzVM4IhCfqXI4rZ+Y8=; 3, 32 -- b=rT+wy8e8+XgZQ7jtRqN+bcQKbY4IZMXCfEKJhkuArlGDF8ynXewOgLTKY1FoPkp1wp+ 3, 32 -- f1yBDS9X1mrXLCFA8usC8l8/UWr3XDiugz3ihURDzQs6nSayhXMRHBrtSmAOW4UyeCgea 3, 32 -- MGr1Lc2lmAtFOx9R07zzgudp+/oJ45JssVg= 3, 32 -- X-RZG-AUTH: :L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBSdqsy65lHRHJMBLi7o7zMYgEOC6u2s = 3, 32 -- X-RZG-CLASS-ID: mo01 3, 32 -- Received: from [192.168.1.6] (host-2-97-120-126.as13285.net [2.97.120.126]) 3, 32 -- by smtp.strato.com (RZmta 37.6 DYNA|AUTH) 3, 32 -- with ESMTPSA id g03805r4M8ldg3u 3, 32 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (curve secp521r1 with 521 ECDH bits, eq. 15360 bits RSA)) 3, 32 -- (Client did not present a certificate) 3, 32 -- for {microscopy-at-microscopy.com} ; 3, 32 -- Fri, 22 May 2015 10:47:39 +0200 (CEST) 3, 32 -- Subject: Gauss meter app for smartphones 3, 32 -- From: Integrity Scientific Ltd {contact-at-integrityscientific.com} 3, 32 -- Content-Type: text/plain; 3, 32 -- charset=us-ascii 3, 32 -- X-Mailer: iPad Mail (12B435) 3, 32 -- Message-Id: {B61EBC4A-7964-4738-8DC5-32B59A2FD11E-at-integrityscientific.com} 3, 32 -- Date: Fri, 22 May 2015 09:47:42 +0100 3, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4M8leIA032312 ==============================End of - Headers==============================
==============================Original Headers============================== 11, 45 -- From les-at-zsgenetics.com Fri May 22 07:33:00 2015 11, 45 -- Received: from gateway22.websitewelcome.com (gateway22.websitewelcome.com [192.185.47.100]) 11, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4MCX0sm016479 11, 45 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2015 07:33:00 -0500 11, 45 -- Received: by gateway22.websitewelcome.com (Postfix, from userid 500) 11, 45 -- id 110BA1323FC35; Fri, 22 May 2015 07:33:00 -0500 (CDT) 11, 45 -- Received: from gator2013.hostgator.com (gator2013.hostgator.com [50.87.144.13]) 11, 45 -- by gateway22.websitewelcome.com (Postfix) with ESMTP id 01F881323FC04 11, 45 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2015 07:33:00 -0500 (CDT) 11, 45 -- Received: from [146.115.5.114] (port=54581 helo=LESZSG) 11, 45 -- by gator2013.hostgator.com with esmtpsa (TLSv1:AES256-SHA:256) 11, 45 -- (Exim 4.82) 11, 45 -- (envelope-from {les-at-zsgenetics.com} ) 11, 45 -- id 1Yvm8J-0004FU-EE; Fri, 22 May 2015 07:32:59 -0500 11, 45 -- From: "Larry Scipioni" {les-at-zsgenetics.com} 11, 45 -- To: {contact-at-integrityscientific.com} 11, 45 -- Cc: {microscopy-at-microscopy.com} 11, 45 -- References: {201505220901.t4M91VZ8016320-at-ns.microscopy.com} 11, 45 -- In-Reply-To: {201505220901.t4M91VZ8016320-at-ns.microscopy.com} 11, 45 -- Subject: RE: [Microscopy] Gauss meter app for smartphones 11, 45 -- Date: Fri, 22 May 2015 08:33:00 -0400 11, 45 -- Organization: ZS Genetics 11, 45 -- Message-ID: {006d01d0948b$711e23b0$535a6b10$-at-zsgenetics.com} 11, 45 -- MIME-Version: 1.0 11, 45 -- Content-Type: text/plain; 11, 45 -- charset="us-ascii" 11, 45 -- Content-Transfer-Encoding: 7bit 11, 45 -- X-Mailer: Microsoft Outlook 14.0 11, 45 -- Thread-Index: AQGUkDZs4DtIrOtZRhpPAN8TGAJbWp3/9EVQ 11, 45 -- Content-Language: en-us 11, 45 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 11, 45 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com 11, 45 -- X-AntiAbuse: Original Domain - microscopy.com 11, 45 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 11, 45 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com 11, 45 -- X-BWhitelist: no 11, 45 -- X-Source-IP: 146.115.5.114 11, 45 -- X-Exim-ID: 1Yvm8J-0004FU-EE 11, 45 -- X-Source: 11, 45 -- X-Source-Args: 11, 45 -- X-Source-Dir: 11, 45 -- X-Source-Sender: (LESZSG) [146.115.5.114]:54581 11, 45 -- X-Source-Auth: les-at-zsgenetics.com 11, 45 -- X-Email-Count: 5 11, 45 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20= ==============================End of - Headers==============================
On May 21, 2015, at 6:06 PM, microscopylistserver- noreply-at-microscopy.com wrote:
} Email: fxwatanabe-at-ualr.edu } Name: Fumiya Watanabe } } Organization: UALR Center for Integrative Nanotechnology Sciences } } Title-Subject: [Filtered] non-radioactive uranly acetate } } Message: Hello Listers, } } I just read a post on uranyl acetate and wanted ti ask you a } question regarding a particular uranyl } acetate sold by EMS as non radioactive. Does this work same as } radioactive ones? Has anybody used } this non-radioactive uranyl acetate? Does it have any kind of } limitations? } } Their description is at this website: } http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx#22400 } } } } Unicryl Embedding and Staining Kit } } Please see listing in Embedding Media Kits. } RT 14660 kit 300.00 Add to Cart } msdsarrowUranyl Acetate, Reagent, A.C.S. } } UO2(OCOCH3)2ˇ2H2O F.W. 424.14 } CAS #541-09-3 } Assay 98.0-102.0% } Specifications: } Insoluble Matters 0.01 } Chloride 0.003% } Sulfate 0.01% } Alkalies (as SO4) 0.05% } Heavy Metals 0.002% } Iron 0.001% } } A universal EM stain for thin sections, en-block staining, and } negative staining. } RT 22400 25g 155.00 Add to Cart } } Dear Fumiya, Uranyl acetate is always radioactive. There are uranium isotopes whose decay rates are quite low, and in some states, the total radioactivity is deemed low enough so that UAc can be discarded down the sink. I expect that this is what EMS has in mind. Yours, Bill
==============================Original Headers============================== 7, 33 -- From wtivol-at-sbcglobal.net Fri May 22 17:41:00 2015 7, 33 -- Received: from nm14.access.bullet.mail.gq1.yahoo.com (nm14.access.bullet.mail.gq1.yahoo.com [216.39.62.45]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4MMexjG020606 7, 33 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2015 17:40:59 -0500 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1432334459; bh=LLmOFlNk0DXsxiUMwvO1Ks007+4NvK3yQeuof0L6LA8=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=QYIlUql+KP7kM5TCx8+R0JkqXAq2M6KRSCMZXQ8dO3S8RUNAu7duy/AJrs/EluQlbjXONGw9KEcYqvpOr/nR9Mti18xss6vULtZZFlsbsWaT0VHIVcTIT3CFe4yBzsecf80ZWcyTyDfNwV17h/vGK6x7eky22xOLP/8+TWyEjWTAQXWDZrrVtQJodEAVrcTfoaTi4juNzqjVS9s79aH6bjGGftao75T0LJXPu1w6QsIhK5Pl7vu29GtU8YyiK4HRVX4B1eaZ3GfnYoy3Ye5vZ1QfbUUzUBkKu4HA8V3UkLtA8snPFBqIjGkoZ6JvECJ9H478bW2hSll8HFt/O9GUXA== 7, 33 -- Received: from [216.39.60.168] by nm14.access.bullet.mail.gq1.yahoo.com with NNFMP; 22 May 2015 22:40:59 -0000 7, 33 -- Received: from [67.195.23.148] by tm4.access.bullet.mail.gq1.yahoo.com with NNFMP; 22 May 2015 22:40:59 -0000 7, 33 -- Received: from [127.0.0.1] by smtp120.sbc.mail.gq1.yahoo.com with NNFMP; 22 May 2015 22:40:59 -0000 7, 33 -- X-Yahoo-Newman-Id: 266864.17574.bm-at-smtp120.sbc.mail.gq1.yahoo.com 7, 33 -- X-Yahoo-Newman-Property: ymail-3 7, 33 -- X-YMail-OSG: WmhCRVoVM1nclPZGGE4Qxf642NTg_TfO3mSOQPcxSqoy26K 7, 33 -- 2FIe_4er1xFiDk0Drr4vnevvb3Qp4sm60MkT2RRT_t.6wZRhF6UKvBOB0aXV 7, 33 -- CzofruEh_rt4Utk4n5a_NMZKoovyG8D4uhLZ5cF3MJtVzCV5eFSlxUakgBtZ 7, 33 -- TN6tjggWAjeSs7POplLRaIvOYoiFIm3lFlYPp8yMGEdupYt3QUevE0f2HrAG 7, 33 -- JZZ.wkZx7vNBWOH8d42agN0qrgQeqB3XXIslCgUUJdw3Oi2uTRzdI8.NyX0v 7, 33 -- IEWqGrhE2rBDcW6_bmdkrToNe0LvhcSQiBQWrswK38.DlO0UPvtuaqyv7BqV 7, 33 -- n8_IwabRyhpwCAIC5qQD4Mh1OUsxSpi55ogP9zYF05YUH5nnDkSaHk7T9tlw 7, 33 -- TrlRQ.Lk67aL.o4ZWjnuM9gDyEYQJ.xRkR0WO9U.Nd8oehCdOa717hgbheuS 7, 33 -- TVRTOrzktFl9epiCbsga.YESlrOl_VFNWlR0C.tMWxw9j39N9Uj7KPCc3hLu 7, 33 -- Nr5iO73m9iMrzUNf9a7gI3tdtw8nw0.Sk3rR4pBPmqQ1aeA8EI62s 7, 33 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 7, 33 -- Message-Id: {64ADFD8E-0BC5-4F29-8DDD-A276241CFF97-at-sbcglobal.net} 7, 33 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 7, 33 -- To: microscopy-at-microscopy.com, fxwatanabe-at-ualr.edu 7, 33 -- In-Reply-To: {201505220106.t4M16R9X011072-at-ns.microscopy.com} 7, 33 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 7, 33 -- Mime-Version: 1.0 (Apple Message framework v936) 7, 33 -- Subject: Re: [Microscopy] viaWWW:non-radioactive uranly acetate 7, 33 -- Date: Fri, 22 May 2015 15:40:57 -0700 7, 33 -- References: {201505220106.t4M16R9X011072-at-ns.microscopy.com} 7, 33 -- X-Mailer: Apple Mail (2.936) 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4MMexjG020606 ==============================End of - Headers==============================
As mentioned by Bill uranyl acetate is at best depleted, but never non-radioactive. What you came across is actually a non-radioactive uranyl acetate (!) substitute (!), which consist of "Two lanthanide salts, samarium and gadolinium triacetate". Check the paper quoted in the data sheet. As a general note, the staining properties of UAc vary substantially between manufacturers and thus between countries (as nobody wants to ship the stuff unless necessary). Testing is the only way to find out. Best Chris
} On May 22, 2015, at 8:03 PM, wtivol-at-sbcglobal.net wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On May 21, 2015, at 6:06 PM, microscopylistserver- } noreply-at-microscopy.com wrote: } } } Email: fxwatanabe-at-ualr.edu } } Name: Fumiya Watanabe } } } } Organization: UALR Center for Integrative Nanotechnology Sciences } } } } Title-Subject: [Filtered] non-radioactive uranly acetate } } } } Message: Hello Listers, } } } } I just read a post on uranyl acetate and wanted ti ask you a } } question regarding a particular uranyl } } acetate sold by EMS as non radioactive. Does this work same as } } radioactive ones? Has anybody used } } this non-radioactive uranyl acetate? Does it have any kind of } } limitations? } } } } Their description is at this website: } } http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx#22400 } } } } } } } } Unicryl Embedding and Staining Kit } } } } Please see listing in Embedding Media Kits. } } RT 14660 kit 300.00 Add to Cart } } msdsarrowUranyl Acetate, Reagent, A.C.S. } } } } UO2(OCOCH3)2¡2H2O F.W. 424.14 } } CAS #541-09-3 } } Assay 98.0-102.0% } } Specifications: } } Insoluble Matters 0.01 } } Chloride 0.003% } } Sulfate 0.01% } } Alkalies (as SO4) 0.05% } } Heavy Metals 0.002% } } Iron 0.001% } } } } A universal EM stain for thin sections, en-block staining, and } } negative staining. } } RT 22400 25g 155.00 Add to Cart } } } } } Dear Fumiya, } Uranyl acetate is always radioactive. There are uranium isotopes } whose decay rates are quite low, and in some states, the total } radioactivity is deemed low enough so that UAc can be discarded down } the sink. I expect that this is what EMS has in mind. } Yours, } Bill } } } } } } ==============================Original Headers============================== } 7, 33 -- From wtivol-at-sbcglobal.net Fri May 22 17:41:00 2015 } 7, 33 -- Received: from nm14.access.bullet.mail.gq1.yahoo.com (nm14.access.bullet.mail.gq1.yahoo.com [216.39.62.45]) } 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4MMexjG020606 } 7, 33 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2015 17:40:59 -0500 } 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1432334459; bh=LLmOFlNk0DXsxiUMwvO1Ks007+4NvK3yQeuof0L6LA8=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=QYIlUql+KP7kM5TCx8+R0JkqXAq2M6KRSCMZXQ8dO3S8RUNAu7duy/AJrs/EluQlbjXONGw9KEcYqvpOr/nR9Mti18xss6vULtZZFlsbsWaT0VHIVcTIT3CFe4yBzsecf80ZWcyTyDfNwV17h/vGK6x7eky22xOLP/8+TWyEjWTAQXWDZrrVtQJodEAVrcTfoaTi4juNzqjVS9s79aH6bjGGftao75T0LJXPu1w6QsIhK5Pl7vu29GtU8YyiK4HRVX4B1eaZ3GfnYoy3Ye5vZ1QfbUUzUBkKu4HA8V3UkLtA8snPFBqIjGkoZ6JvECJ9H478bW2hSll8HFt/O9GUXA== } 7, 33 -- Received: from [216.39.60.168] by nm14.access.bullet.mail.gq1.yahoo.com with NNFMP; 22 May 2015 22:40:59 -0000 } 7, 33 -- Received: from [67.195.23.148] by tm4.access.bullet.mail.gq1.yahoo.com with NNFMP; 22 May 2015 22:40:59 -0000 } 7, 33 -- Received: from [127.0.0.1] by smtp120.sbc.mail.gq1.yahoo.com with NNFMP; 22 May 2015 22:40:59 -0000 } 7, 33 -- X-Yahoo-Newman-Id: 266864.17574.bm-at-smtp120.sbc.mail.gq1.yahoo.com } 7, 33 -- X-Yahoo-Newman-Property: ymail-3 } 7, 33 -- X-YMail-OSG: WmhCRVoVM1nclPZGGE4Qxf642NTg_TfO3mSOQPcxSqoy26K } 7, 33 -- 2FIe_4er1xFiDk0Drr4vnevvb3Qp4sm60MkT2RRT_t.6wZRhF6UKvBOB0aXV } 7, 33 -- CzofruEh_rt4Utk4n5a_NMZKoovyG8D4uhLZ5cF3MJtVzCV5eFSlxUakgBtZ } 7, 33 -- TN6tjggWAjeSs7POplLRaIvOYoiFIm3lFlYPp8yMGEdupYt3QUevE0f2HrAG } 7, 33 -- JZZ.wkZx7vNBWOH8d42agN0qrgQeqB3XXIslCgUUJdw3Oi2uTRzdI8.NyX0v } 7, 33 -- IEWqGrhE2rBDcW6_bmdkrToNe0LvhcSQiBQWrswK38.DlO0UPvtuaqyv7BqV } 7, 33 -- n8_IwabRyhpwCAIC5qQD4Mh1OUsxSpi55ogP9zYF05YUH5nnDkSaHk7T9tlw } 7, 33 -- TrlRQ.Lk67aL.o4ZWjnuM9gDyEYQJ.xRkR0WO9U.Nd8oehCdOa717hgbheuS } 7, 33 -- TVRTOrzktFl9epiCbsga.YESlrOl_VFNWlR0C.tMWxw9j39N9Uj7KPCc3hLu } 7, 33 -- Nr5iO73m9iMrzUNf9a7gI3tdtw8nw0.Sk3rR4pBPmqQ1aeA8EI62s } 7, 33 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t } 7, 33 -- Message-Id: {64ADFD8E-0BC5-4F29-8DDD-A276241CFF97-at-sbcglobal.net} } 7, 33 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} } 7, 33 -- To: microscopy-at-microscopy.com, fxwatanabe-at-ualr.edu } 7, 33 -- In-Reply-To: {201505220106.t4M16R9X011072-at-ns.microscopy.com} } 7, 33 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes } 7, 33 -- Mime-Version: 1.0 (Apple Message framework v936) } 7, 33 -- Subject: Re: [Microscopy] viaWWW:non-radioactive uranly acetate } 7, 33 -- Date: Fri, 22 May 2015 15:40:57 -0700 } 7, 33 -- References: {201505220106.t4M16R9X011072-at-ns.microscopy.com} } 7, 33 -- X-Mailer: Apple Mail (2.936) } 7, 33 -- Content-Transfer-Encoding: 8bit } 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4MMexjG020606 } ==============================End of - Headers==============================
==============================Original Headers============================== 4, 37 -- From cbuser125-at-gmail.com Fri May 22 18:55:44 2015 4, 37 -- Received: from mail-qg0-f48.google.com (mail-qg0-f48.google.com [209.85.192.48]) 4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4MNtiv3009825 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2015 18:55:44 -0500 4, 37 -- Received: by qgfa63 with SMTP id a63so18039841qgf.0 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2015 16:55:43 -0700 (PDT) 4, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 37 -- d=gmail.com; s=20120113; 4, 37 -- h=from:content-type:content-transfer-encoding:mime-version:subject 4, 37 -- :message-id:date:references:in-reply-to:to; 4, 37 -- bh=oIa0vhlv1eIJS7JdxHBMxaPXUegZ3diL/4G5Y8t6244=; 4, 37 -- b=hCPqhTMj1N1pPwwXN9DuMAk2wibteHlEzKk8fKopBjBbSBBechAdeQmW5iJiLpyM+D 4, 37 -- u9INeqX119qms74+41yg7q92XLc//AcoCg0FrND39tmLT0KJs+WwsWDfTdjTEsrikCRd 4, 37 -- jPSwmTZoPyx7dOVEzAbhCD8AtY7hhPIZvUVTM7TmkmBBHW1ZQbojRawcCbj+1CPYU6NU 4, 37 -- g9NGUK5JAMOVZlA3bi7iYoO52hO9swPdg6oKeHYhqfWTt3Q6ZmYufdP52zOgaN64saJw 4, 37 -- Gptpchj31jCT4bsWmyfwyGbMfZiQeIJDtpBziW8gmqTiH2PYwv2pyetz3W8luOom36gL 4, 37 -- MmMQ== 4, 37 -- X-Received: by 10.55.53.72 with SMTP id c69mr23651787qka.67.1432338515700; 4, 37 -- Fri, 22 May 2015 16:48:35 -0700 (PDT) 4, 37 -- Received: from [172.16.0.18] ([200.61.9.66]) 4, 37 -- by mx.google.com with ESMTPSA id k69sm2293269qkh.48.2015.05.22.16.48.34 4, 37 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 4, 37 -- Fri, 22 May 2015 16:48:35 -0700 (PDT) 4, 37 -- From: Chris Buser {cbuser125-at-gmail.com} 4, 37 -- Content-Type: text/plain; 4, 37 -- charset=utf-8 4, 37 -- Mime-Version: 1.0 (1.0) 4, 37 -- Subject: Re: [Microscopy] Re: viaWWW:non-radioactive uranly acetate 4, 37 -- Message-Id: {ED6197C2-278E-41B3-B39F-5E3341EA6AB8-at-gmail.com} 4, 37 -- Date: Fri, 22 May 2015 20:48:32 -0300 4, 37 -- References: {201505222303.t4MN3GsQ008691-at-ns.microscopy.com} 4, 37 -- In-Reply-To: {201505222303.t4MN3GsQ008691-at-ns.microscopy.com} 4, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , 4, 37 -- "fxwatanabe-at-ualr.edu" {fxwatanabe-at-ualr.edu} 4, 37 -- X-Mailer: iPhone Mail (12F70) 4, 37 -- Content-Transfer-Encoding: 8bit 4, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4MNtiv3009825 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45ds-at-gmail.com Sat May 23 20:18:44 2015 Return-Path: {mike.sfsd4f564s6df45ds-at-gmail.com} Received: from gmail.com ([175.119.156.122]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t4O1IeAP010776 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 23 May 2015 20:18:42 -0500 Message-ID: {AB74902D.ACEE4BB6-at-gmail.com}
Hello All, We have a trouble with our old Philips CM100. The info CRT has gone. About two weeks the image on it was very faint, but still resolvable. Now the screen is completely dark. The problem is not in dimming circuits because the dimming of panel's LEDs is working fine and can be adjusted. I think that the problem is in CRT tube. Please does anybody had to solve such problem? I find out that CRT tube (M24-306WR/ED) is still available on the market.
Thanking for any response in advance.
Oldrich
P.S. No response from service, up to now.
Upozorneni: Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.
Disclaimer: If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR.
==============================Original Headers============================== 8, 40 -- From benada-at-biomed.cas.cz Mon May 25 03:37:07 2015 8, 40 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4P8b788015965 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 03:37:07 -0500 8, 40 -- X-ASG-Debug-ID: 1432543025-05011e2f78029f0001-4CH8be 8, 40 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id 4KjfoH6y4W5u4NE8 (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 10:37:05 +0200 (CEST) 8, 40 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 8, 40 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 8, 40 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) 8, 40 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 8, 40 -- (No client certificate requested) 8, 40 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id B1E092C80E9 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 10:37:01 +0200 (CEST) 8, 40 -- Date: Mon, 25 May 2015 10:37:00 +0200 8, 40 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 8, 40 -- To: {microscopy-at-microscopy.com} 8, 40 -- Subject: Philips CM100 trouble 8, 40 -- Message-ID: {20150525103700.0158a2ff-at-u117fs} 8, 40 -- X-ASG-Orig-Subj: Philips CM100 trouble 8, 40 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 8, 40 -- =?UTF-8?B?xIxS?= 8, 40 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Type: text/plain; charset=US-ASCII 8, 40 -- Content-Transfer-Encoding: 7bit 8, 40 -- X-IoP-CAS-MailScanner-ID: B1E092C80E9.36113 8, 40 -- X-IoP-CAS-MailScanner: Processed 8, 40 -- X-Spam-Status: No 8, 40 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 8, 40 -- X-Barracuda-Start-Time: 1432543025 8, 40 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 8, 40 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 8, 40 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 8, 40 -- X-Barracuda-BRTS-Status: 1 8, 40 -- X-Barracuda-Spam-Score: 0.00 8, 40 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 8, 40 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.19260 8, 40 -- Rule breakdown below 8, 40 -- pts rule name description 8, 40 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
Replacing CRT is a straight-forward task which is well within the scope of skills for any decent TV repair technician... look for old-timers who have actually seen a CRT. To verify that problem is really with CRT and not malfunction of the dimming circuit you would need help of the EE. Find one local who is adequately qualified, isn't be afraid touching TEM, and figure out ways of paying him for doing the job.
Good luck!
Valery Valery Ray - also with AIM Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
On 5/25/2015 4:39 AM, benada-at-biomed.cas.cz wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello All, } We have a trouble with our old Philips CM100. The info CRT has gone. } About two weeks the image on it was very faint, but still resolvable. } Now the screen is completely dark. The problem is not in dimming } circuits because the dimming of panel's LEDs is working fine and can be } adjusted. I think that the problem is in CRT tube. Please does anybody } had to solve such problem? I find out that CRT tube (M24-306WR/ED) is } still available on the market. } } Thanking for any response in advance. } } Oldrich } } P.S. No response from service, up to now. } } } Upozorneni: } Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i. } } Disclaimer: } If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR. } } } ==============================Original Headers============================== } 8, 40 -- From benada-at-biomed.cas.cz Mon May 25 03:37:07 2015 } 8, 40 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) } 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4P8b788015965 } 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 03:37:07 -0500 } 8, 40 -- X-ASG-Debug-ID: 1432543025-05011e2f78029f0001-4CH8be } 8, 40 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id 4KjfoH6y4W5u4NE8 (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 10:37:05 +0200 (CEST) } 8, 40 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz } 8, 40 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 } 8, 40 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) } 8, 40 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) } 8, 40 -- (No client certificate requested) } 8, 40 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id B1E092C80E9 } 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 10:37:01 +0200 (CEST) } 8, 40 -- Date: Mon, 25 May 2015 10:37:00 +0200 } 8, 40 -- From: Oldrich Benada {benada-at-biomed.cas.cz} } 8, 40 -- To: {microscopy-at-microscopy.com} } 8, 40 -- Subject: Philips CM100 trouble } 8, 40 -- Message-ID: {20150525103700.0158a2ff-at-u117fs} } 8, 40 -- X-ASG-Orig-Subj: Philips CM100 trouble } 8, 40 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV } 8, 40 -- =?UTF-8?B?xIxS?= } 8, 40 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) } 8, 40 -- MIME-Version: 1.0 } 8, 40 -- Content-Type: text/plain; charset=US-ASCII } 8, 40 -- Content-Transfer-Encoding: 7bit } 8, 40 -- X-IoP-CAS-MailScanner-ID: B1E092C80E9.36113 } 8, 40 -- X-IoP-CAS-MailScanner: Processed } 8, 40 -- X-Spam-Status: No } 8, 40 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] } 8, 40 -- X-Barracuda-Start-Time: 1432543025 } 8, 40 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA } 8, 40 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi } 8, 40 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz } 8, 40 -- X-Barracuda-BRTS-Status: 1 } 8, 40 -- X-Barracuda-Spam-Score: 0.00 } 8, 40 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= } 8, 40 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.19260 } 8, 40 -- Rule breakdown below } 8, 40 -- pts rule name description } 8, 40 -- ---- ---------------------- -------------------------------------------------- } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 32 -- From vray-at-partbeamsystech.com Mon May 25 08:31:11 2015 5, 32 -- Received: from nm12-vm0.bullet.mail.bf1.yahoo.com (nm12-vm0.bullet.mail.bf1.yahoo.com [98.139.213.140]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4PDVBHF012830 5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 08:31:11 -0500 5, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1432560670; bh=VNBzQYmuoumL/kRrtZpNmexFACxtttFGY8s/peQjGTs=; h=Date:From:To:Subject:References:In-Reply-To:From:Subject; b=IB0WIlo8n7g7KFkxyj5LqkQAQlQqCpqjpFM7S2AVtLWa7DTGhG7r609acyuDQ4eD95rSzMKPk1rw/mM1LBjSl8/QebCZfYh6jspdjA9kP6JDwRb1eh/xMmiRF+5t/NRFnFu6CZCgR6QXAAv7tRNUc8bXPE7Y771iDVkdjOLy3w6JiLtT4aGhoEnG91klGKk5HUzNfv+vH3hNCZDEBB0QVGP9pQw/iBguXX0VkZFS0fFdSQPFhk8H7aCUJNeO9XCxWq3ivRr5pzfSvjf6sWw7EU4JQpFfPlcxy5s8F2uh+1jM/qQ41HB6bhvycT03CkugDvvLfSLRgWAaGRcyIZ6Pew== 5, 32 -- Received: from [66.196.81.172] by nm12.bullet.mail.bf1.yahoo.com with NNFMP; 25 May 2015 13:31:10 -0000 5, 32 -- Received: from [98.139.213.11] by tm18.bullet.mail.bf1.yahoo.com with NNFMP; 25 May 2015 13:31:10 -0000 5, 32 -- Received: from [127.0.0.1] by smtp111.mail.bf1.yahoo.com with NNFMP; 25 May 2015 13:31:10 -0000 5, 32 -- X-Yahoo-Newman-Id: 900142.20303.bm-at-smtp111.mail.bf1.yahoo.com 5, 32 -- X-Yahoo-Newman-Property: ymail-3 5, 32 -- X-YMail-OSG: Pf5zpAoVM1np5cHvlkrzdTxfvwdeLJ76wXOZMTRMViJRfFa 5, 32 -- Q05a.l1yk9enXe5ynx91lXdSKOaZP3KSFNtBSNiLfa7m_C.9F1YOL5PEChyH 5, 32 -- 94VjsO.mFcGkUndHLAzwb5VMfZh6BnutuyzdAQ1ju_2aIizG4ioThhjt3OZe 5, 32 -- bSbN8bdh707brnl4LEOu7AWCbOXMvvtzveB6am2Nr10aFhWWzISQJSyXc5N. 5, 32 -- 7RCCc48qhYoEA72nsdWnCS9DXB1f96N9JLQHCemdis48.1PKz4UkJnvB5UXd 5, 32 -- fdBj9eSPV9rnNX1tmklgoZpjT2FmwYgQvOjs6npUgfL5BW.knKF3wlQ0XPHo 5, 32 -- 22gxLiLqEXkaA6J2QPPsH_a4VW5U05iwlR_OebsyfIfAhhB_NFr0zWz3o6BX 5, 32 -- vkIRqKxINi08FJ.4YWLBoqhxKGBobPF3XLSYLDMbsWzz3Mi1Va9fJkRt3Z7g 5, 32 -- TpoOFmEEO4.RQp8Df_1DcE6qZGiWFMQcQoeIpZRZ2nFC8qKBqKK4H9jjC86Y 5, 32 -- E13ZouAWaP1ZLPLI0zR0aFlY_8RqBnLK1yMLDWVdu1A-- 5, 32 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 32 -- Message-ID: {5563241E.6020300-at-partbeamsystech.com} 5, 32 -- Date: Mon, 25 May 2015 09:31:10 -0400 5, 32 -- From: Valery Ray {vray-at-partbeamsystech.com} 5, 32 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 5, 32 -- MIME-Version: 1.0 5, 32 -- To: benada-at-biomed.cas.cz, microscopy-at-microscopy.com 5, 32 -- Subject: Re: [Microscopy] Philips CM100 trouble 5, 32 -- References: {201505250839.t4P8dBoO017121-at-ns.microscopy.com} 5, 32 -- In-Reply-To: {201505250839.t4P8dBoO017121-at-ns.microscopy.com} 5, 32 -- Content-Type: text/plain; charset=windows-1252; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mk-at-seclab.tuwien.ac.at as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mk-at-seclab.tuwien.ac.at Name: Markus Kammerstetter
Organization: Secure Systems Lab Vienna, Vienna University of Technology
Title-Subject: [Filtered] Looking for affordable second-hand FIB
Message: Hello,
we are a small lab at Vienna University of Technology working in the field of Hardware and Integrated Circuit Security. Due to a lack of funding, a major part of our lab equipment is second hand and has been jointly funded by lab members so far. As a result, we mostly work with second-hand equipment (i.e. a customized digital SEM, a plasma etcher, a custom motorized confocal microscope, a sputter coater, a probe station, a wedge bonder, a CMP polisher, etc.). To fit our needs, we enjoy working on constantly improving, modernizing and/or customizing our tools, for instance by developing custom hard- and software, adding newer system components or machining custom parts. While our equipment is not state of the art, we see the possibility to make these adaptions and customizations as huge advantage allowing us to fit our tools to our specific applications.
In that regard, we are looking for an affordable second-hand Focused Ion Beam (FIB) system which we plan to use for circuit edits on integrated circuits. This involves milling through passivation/insulation layers (SiO2 or Si3N4) as well as metal layers (e.g. Cu or Al). Using precursor gas based deposition, the next step involves metal and insulator deposition to re-wire signals on the die or to make probe pads which we can electrically contact with our probe station and tungsten microprobe needles on micropositioners.
Ideally, the FIB should have 2 GIS systems installed for the deposition of a metal (e.g. tungsten) and an insulator (SiO2). For instance, a system as old as a single-beam FEI FIB 200 would still work well for us and we would be more than happy to have it at our lab.
If you tend to have an older model FIB at your facility and you're planning to sell, it would be great if you could drop me a line.
Thank you and best regards, Markus
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==============================Original Headers============================== 24, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon May 25 09:23:53 2015 24, 28 -- Received: from znl.com ([206.69.208.20]) 24, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4PENrOK003148 24, 28 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 09:23:53 -0500 24, 28 -- Received: from localhost (localhost [127.0.0.1]) 24, 28 -- by znl.com (Postfix) with ESMTP id 3F73951A5B3 24, 28 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 09:23:53 -0500 (CDT) 24, 28 -- X-Virus-Scanned: amavisd-new at znl.com 24, 28 -- Received: from znl.com ([127.0.0.1]) 24, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 24, 28 -- with ESMTP id ji8O1Y27fyqI for {microscopy-at-microscopy.com} ; 24, 28 -- Mon, 25 May 2015 09:23:38 -0500 (CDT) 24, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 24, 28 -- by znl.com (Postfix) with ESMTPA id 545E251A5A0 24, 28 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 09:23:38 -0500 (CDT) 24, 28 -- Message-ID: {55633063.1020803-at-microscopy.com} 24, 28 -- Date: Mon, 25 May 2015 09:23:31 -0500 24, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 24, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 24, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 24, 28 -- MIME-Version: 1.0 24, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 24, 28 -- Subject: viaWWW:Looking for affordable second-hand FIB 24, 28 -- References: {201505251010.t4PAAGIo006536-at-ns.microscopy.com} 24, 28 -- In-Reply-To: {201505251010.t4PAAGIo006536-at-ns.microscopy.com} 24, 28 -- X-Forwarded-Message-Id: {201505251010.t4PAAGIo006536-at-ns.microscopy.com} 24, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 24, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I just did a quick Google search on the part number and found this website... http://crtsolutions.highwire.com/product/m24-306wred
For $200US you can swap the CRT and verify whether it is indeed the tube. Even if it is a different part of the circuit, at least you will have a spare tube since they do have a finite lifetime.
No financial interest in the vendor. It was the 1st one on the Google search list...
Cheers, Henk
On 5/25/2015 4:39 AM, benada-at-biomed.cas.cz wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello All, } We have a trouble with our old Philips CM100. The info CRT has gone. } About two weeks the image on it was very faint, but still resolvable. } Now the screen is completely dark. The problem is not in dimming } circuits because the dimming of panel's LEDs is working fine and can be } adjusted. I think that the problem is in CRT tube. Please does anybody } had to solve such problem? I find out that CRT tube (M24-306WR/ED) is } still available on the market. } } Thanking for any response in advance. } } Oldrich } } P.S. No response from service, up to now. } } } Upozorneni: } Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i. } } Disclaimer: } If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR. } } } ==============================Original Headers============================== } 8, 40 -- From benada-at-biomed.cas.cz Mon May 25 03:37:07 2015 } 8, 40 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) } 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4P8b788015965 } 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 03:37:07 -0500 } 8, 40 -- X-ASG-Debug-ID: 1432543025-05011e2f78029f0001-4CH8be } 8, 40 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id 4KjfoH6y4W5u4NE8 (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 10:37:05 +0200 (CEST) } 8, 40 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz } 8, 40 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 } 8, 40 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) } 8, 40 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) } 8, 40 -- (No client certificate requested) } 8, 40 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id B1E092C80E9 } 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 10:37:01 +0200 (CEST) } 8, 40 -- Date: Mon, 25 May 2015 10:37:00 +0200 } 8, 40 -- From: Oldrich Benada {benada-at-biomed.cas.cz} } 8, 40 -- To: {microscopy-at-microscopy.com} } 8, 40 -- Subject: Philips CM100 trouble } 8, 40 -- Message-ID: {20150525103700.0158a2ff-at-u117fs} } 8, 40 -- X-ASG-Orig-Subj: Philips CM100 trouble } 8, 40 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV } 8, 40 -- =?UTF-8?B?xIxS?= } 8, 40 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) } 8, 40 -- MIME-Version: 1.0 } 8, 40 -- Content-Type: text/plain; charset=US-ASCII } 8, 40 -- Content-Transfer-Encoding: 7bit } 8, 40 -- X-IoP-CAS-MailScanner-ID: B1E092C80E9.36113 } 8, 40 -- X-IoP-CAS-MailScanner: Processed } 8, 40 -- X-Spam-Status: No } 8, 40 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] } 8, 40 -- X-Barracuda-Start-Time: 1432543025 } 8, 40 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA } 8, 40 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi } 8, 40 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz } 8, 40 -- X-Barracuda-BRTS-Status: 1 } 8, 40 -- X-Barracuda-Spam-Score: 0.00 } 8, 40 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= } 8, 40 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.19260 } 8, 40 -- Rule breakdown below } 8, 40 -- pts rule name description } 8, 40 -- ---- ---------------------- -------------------------------------------------- } ==============================End of - Headers==============================
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
Replacing a crt is a pretty non-trivial task when they do not include the yoke. The problem is screen geometry. There are tiny magnets installed with glue or tape on the yoke and crt that help compensate for irregularities in the magnetic field driving the scan of the crt and to compensate for variances in crt manufacture. You have to adjust the yoke while the machine is on and running which is a bit dangerous.
Before you mess with the crt you need to make sure it is not an issue in the drive. Bad HV from the flyback could cause this as well as a bad transistor in the video drive. You will need a high voltage probe to check the voltage at the anode cap and a o-scope to check the video signals.
-Jerry
} On May 25, 2015, at 9:46 AM, colijn.1-at-osu.edu wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Oldrich, } } I just did a quick Google search on the part number and found this } website... } http://crtsolutions.highwire.com/product/m24-306wred } } For $200US you can swap the CRT and verify whether it is indeed the } tube. Even if it is a different part of the circuit, at least you will } have a spare tube since they do have a finite lifetime. } } No financial interest in the vendor. It was the 1st one on the Google } search list... } } Cheers, } Henk } } } } On 5/25/2015 4:39 AM, benada-at-biomed.cas.cz wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hello All, } } We have a trouble with our old Philips CM100. The info CRT has gone. } } About two weeks the image on it was very faint, but still resolvable. } } Now the screen is completely dark. The problem is not in dimming } } circuits because the dimming of panel's LEDs is working fine and can be } } adjusted. I think that the problem is in CRT tube. Please does anybody } } had to solve such problem? I find out that CRT tube (M24-306WR/ED) is } } still available on the market. } } } } Thanking for any response in advance. } } } } Oldrich } } } } P.S. No response from service, up to now. } } } } } } Upozorneni: } } Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i. } } } } Disclaimer: } } If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR. } } } } } } ==============================Original Headers============================== } } 8, 40 -- From benada-at-biomed.cas.cz Mon May 25 03:37:07 2015 } } 8, 40 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) } } 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4P8b788015965 } } 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 03:37:07 -0500 } } 8, 40 -- X-ASG-Debug-ID: 1432543025-05011e2f78029f0001-4CH8be } } 8, 40 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id 4KjfoH6y4W5u4NE8 (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 10:37:05 +0200 (CEST) } } 8, 40 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz } } 8, 40 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 } } 8, 40 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) } } 8, 40 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) } } 8, 40 -- (No client certificate requested) } } 8, 40 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id B1E092C80E9 } } 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 10:37:01 +0200 (CEST) } } 8, 40 -- Date: Mon, 25 May 2015 10:37:00 +0200 } } 8, 40 -- From: Oldrich Benada {benada-at-biomed.cas.cz} } } 8, 40 -- To: {microscopy-at-microscopy.com} } } 8, 40 -- Subject: Philips CM100 trouble } } 8, 40 -- Message-ID: {20150525103700.0158a2ff-at-u117fs} } } 8, 40 -- X-ASG-Orig-Subj: Philips CM100 trouble } } 8, 40 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV } } 8, 40 -- =?UTF-8?B?xIxS?= } } 8, 40 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) } } 8, 40 -- MIME-Version: 1.0 } } 8, 40 -- Content-Type: text/plain; charset=US-ASCII } } 8, 40 -- Content-Transfer-Encoding: 7bit } } 8, 40 -- X-IoP-CAS-MailScanner-ID: B1E092C80E9.36113 } } 8, 40 -- X-IoP-CAS-MailScanner: Processed } } 8, 40 -- X-Spam-Status: No } } 8, 40 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] } } 8, 40 -- X-Barracuda-Start-Time: 1432543025 } } 8, 40 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA } } 8, 40 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi } } 8, 40 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz } } 8, 40 -- X-Barracuda-BRTS-Status: 1 } } 8, 40 -- X-Barracuda-Spam-Score: 0.00 } } 8, 40 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= } } 8, 40 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.19260 } } 8, 40 -- Rule breakdown below } } 8, 40 -- pts rule name description } } 8, 40 -- ---- ---------------------- -------------------------------------------------- } } ==============================End of - Headers============================== } } -- } } Hendrik O. Colijn } } *C*enter for *E*lectron *M*icroscopy & *A*nalysi*S* } } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212 } } colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} 614.643.3458 Office } } cemas.osu.edu } } "Time is that quality of nature which keeps things from happening all at } once. Lately it doesn't seem to be working." } } } ==============================Original Headers============================== } 15, 93 -- From colijn.1-at-osu.edu Mon May 25 11:10:43 2015 } 15, 93 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1bon0138.outbound.protection.outlook.com [157.56.111.138]) } 15, 93 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4PGAfHT031392 } 15, 93 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 11:10:43 -0500 } 15, 93 -- Received: from BN1BFFO11FD001.protection.gbl (10.58.144.34) by } 15, 93 -- BN1BFFO11HUB029.protection.gbl (10.58.144.176) with Microsoft SMTP Server } 15, 93 -- (TLS) id 15.1.172.14; Mon, 25 May 2015 16:10:40 +0000 } 15, 93 -- Authentication-Results: spf=pass (sender IP is 164.107.81.216) } 15, 93 -- smtp.mailfrom=osu.edu; biomed.cas.cz; dkim=none (message not signed) } 15, 93 -- header.d=none; } 15, 93 -- Received-SPF: Pass (protection.outlook.com: domain of osu.edu designates } 15, 93 -- 164.107.81.216 as permitted sender) receiver=protection.outlook.com; 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==============================Original Headers============================== 5, 37 -- From jerry.biehler-at-gmail.com Mon May 25 12:37:44 2015 5, 37 -- Received: from mail-pd0-f169.google.com (mail-pd0-f169.google.com [209.85.192.169]) 5, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4PHbhKK020968 5, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 25 May 2015 12:37:44 -0500 5, 37 -- Received: by pdea3 with SMTP id a3so73205262pde.2 5, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 25 May 2015 10:37:43 -0700 (PDT) 5, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 37 -- d=gmail.com; s=20120113; 5, 37 -- h=from:content-type:content-transfer-encoding:mime-version:subject 5, 37 -- :message-id:date:references:in-reply-to:to; 5, 37 -- bh=yiUktj8IVWma3mkUeuLAqDk3+QQOBzsR45i7xZQ7h/E=; 5, 37 -- b=MFxZFx0o/iJ9EPEQsrfxlVNcjxTvi2Ii0jXgNMQY1pfFDhTEh6vvnBkaVntJ37+24+ 5, 37 -- q6+Iz6frNUsf1yMdWNevzZ0MTXfjiL7X+a0gbKOvQ2fe03yDYv8fQd6878puyleXMZX7 5, 37 -- VlDzzEMx0n6FSKqFIRiSNVsML8cdUo4xiy0onCAa/W/H4tIAkx0EAKZTESSGV/RUz/rM 5, 37 -- fDCelRjbBIWKpa47E8umhzrYXdU02e5244Z74OSVVG7VG9W0zFyKp2Hv0RAyimXF3KMf 5, 37 -- 6bDNa0mFmvic4i+6Urrf8938kyBREvAVSNIgzgWPrwypRWyB9IDmkiLWruUe0nB8DPbJ 5, 37 -- tbtQ== 5, 37 -- X-Received: by 10.70.63.1 with SMTP id c1mr41695720pds.90.1432575463173; 5, 37 -- Mon, 25 May 2015 10:37:43 -0700 (PDT) 5, 37 -- Received: from [192.168.1.146] (static-50-53-94-149.bvtn.or.frontiernet.net. [50.53.94.149]) 5, 37 -- by mx.google.com with ESMTPSA id j7sm10612351pbq.35.2015.05.25.10.37.41 5, 37 -- for {Microscopy-at-microscopy.com} 5, 37 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 5, 37 -- Mon, 25 May 2015 10:37:41 -0700 (PDT) 5, 37 -- From: Jerry Biehler {jerry.biehler-at-gmail.com} 5, 37 -- Content-Type: text/plain; 5, 37 -- charset=us-ascii 5, 37 -- Mime-Version: 1.0 (1.0) 5, 37 -- Subject: Re: [Microscopy] Re: Philips CM100 trouble 5, 37 -- Message-Id: {061366F4-F4FF-4C57-BC83-D5BAAD90A1EA-at-gmail.com} 5, 37 -- Date: Mon, 25 May 2015 10:37:40 -0700 5, 37 -- References: {201505251646.t4PGkJDW018485-at-ns.microscopy.com} 5, 37 -- In-Reply-To: {201505251646.t4PGkJDW018485-at-ns.microscopy.com} 5, 37 -- To: Microscopy-at-microscopy.com 5, 37 -- X-Mailer: iPhone Mail (12F70) 5, 37 -- Content-Transfer-Encoding: 8bit 5, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4PHbhKK020968 ==============================End of - Headers==============================
Hi, I recently acquired a New Wave Research ezlaze 532/355 laser microscope but the power supply is missing. Any recommendations for how to acquire a replacement? Maybe someone has a half working system they can part out?
Unit looks like this: https://siliconpr0n.org/wiki/lib/exe/detail.php?id=nwr%3Aezlaze&media=ebay:ntxsupply:151675355993:6.jpg
There is a parts unit on eBay but they are asking outside of my price range so I'm trying to explore other options
John
==============================Original Headers============================== 4, 33 -- From johndmcmaster-at-gmail.com Mon May 25 13:14:26 2015 4, 33 -- Received: from mail-pd0-f173.google.com (mail-pd0-f173.google.com [209.85.192.173]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4PIEQJd009256 4, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 25 May 2015 13:14:26 -0500 4, 33 -- Received: by pdbki1 with SMTP id ki1so32016150pdb.1 4, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 25 May 2015 11:14:26 -0700 (PDT) 4, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 33 -- d=gmail.com; s=20120113; 4, 33 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 33 -- :content-type:content-transfer-encoding; 4, 33 -- bh=NPaRFOf+REh7wD7HGKwElJCdIUftthKvrms3SozUWxg=; 4, 33 -- b=LA+LN0ELnVnIdQNWgjpVxsCvkWgiOTdKU2VyJ89oi9v23NW/xsrxq4qzPnzUPH/Fgz 4, 33 -- RTz3xRC3oFRvv/imek+5Cuzo28hzbo0RgMRqUlPv4Z3zuJr79J1eArHvwznyCj/Z4ehg 4, 33 -- FOg7KCbTEUBgAt5cOyggAjFJ9zGkLTNu9PNGD7MdZpbfw4GG/E01wqUeBkrUCYjrzuEJ 4, 33 -- 9232hsSKTM+syEFJC9af90e3KBOSBmAcGG3FdS2E0iVURVSE6u/99zBmH40qhVrs6Zzn 4, 33 -- JOB4EfaMhndNiAL/dsRZmZZv/qoGBEhM6YehOr77W14S+9V5Nji9rurngLbdBWs4TkVw 4, 33 -- yW0A== 4, 33 -- X-Received: by 10.68.201.138 with SMTP id ka10mr42078384pbc.6.1432577666075; 4, 33 -- Mon, 25 May 2015 11:14:26 -0700 (PDT) 4, 33 -- Received: from [192.168.2.103] (c-67-169-180-245.hsd1.ca.comcast.net. [67.169.180.245]) 4, 33 -- by mx.google.com with ESMTPSA id fs16sm10708565pdb.12.2015.05.25.11.14.25 4, 33 -- for {Microscopy-at-microscopy.com} 4, 33 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 4, 33 -- Mon, 25 May 2015 11:14:25 -0700 (PDT) 4, 33 -- Message-ID: {55636680.3090504-at-gmail.com} 4, 33 -- Date: Mon, 25 May 2015 11:14:24 -0700 4, 33 -- From: John McMaster {johndmcmaster-at-gmail.com} 4, 33 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 4, 33 -- MIME-Version: 1.0 4, 33 -- To: Microscopy-at-microscopy.com 4, 33 -- Subject: Missing ezlaze power supply 4, 33 -- Content-Type: text/plain; charset=utf-8; format=flowed 4, 33 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: lisavandermye-at-adcorp.com.au Name: Lisa van der Mye
Organization: Macquarie University
Title-Subject: [Filtered] Position Opening: Manager (Microscopy Unit)
Message: The Role The Faculty of Science and Engineering is seeking an enthusiastic and talented person to provide expert technical management of the Faculty's Microscopy Unit. Reporting to the Faculty Technical Manager the appointee will manage the Unit and its resources and provide a comprehensive range of technical advice and support across light, fluorescence, confocal and electron microscopy.
For further information regarding this role please view http://jobs.mq.edu.au/cw/en/job/495809/manager-microscopy-unit
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon May 25 22:17:42 2015 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4Q3HgTZ015262 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 22:17:42 -0500 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id 67E33521A8F 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 22:17:42 -0500 (CDT) 15, 28 -- X-Virus-Scanned: amavisd-new at znl.com 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id NQ2Yp5k4sFgY for {microscopy-at-microscopy.com} ; 15, 28 -- Mon, 25 May 2015 22:17:27 -0500 (CDT) 15, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 48112521A7D 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 25 May 2015 22:17:27 -0500 (CDT) 15, 28 -- Message-ID: {5563E5C6.7050200-at-microscopy.com} 15, 28 -- Date: Mon, 25 May 2015 22:17:26 -0500 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:Position Opening: Manager (Microscopy Unit) Macquarie University 15, 28 -- References: {201505252343.t4PNhsbD023663-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201505252343.t4PNhsbD023663-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201505252343.t4PNhsbD023663-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
As Research Scientist, Microscopy, you will engage with a diverse range of students, postdocs, staff scientists and international visitors, in training, mentoring and collaborative roles. You will also maintain and develop microscopy infrastructure to enable current research and plan for future work. This is a particularly wide-ranging role, requiring both a deep knowledge of imaging and a broad background in plant biology.
First two requirements: A doctorate or equivalent research experience in plant cell and/or molecular biology. Postgraduate or equivalent research experience, reflected in co-authorship of publications in plant structure and biology, requiring use of fluorescence and/or confocal microscopy and/or scanning or transmission electron microscopy.
Please visit http://csiro.nga.net.au/cp/index.cfm?event=jobs.jati&returnToEvent=jobs.hom e&jobID=14145619-bd01-8553-6b48-88b975168783&audienceTypeCode=EXT&UseAudien ceTypeLanguage=1 for full details about the position and to find out how to apply.
If this link is broken, visit http://csiro.nga.net.au Select Research Scientists and Engineers. Find this position in the list.
Closing date 28 June, 2015
Dr Rosemary White Manager, Microscopy Centre CSIRO Agriculture, Black Mountain GPO Box 1600 Canberra, ACT 2601
T 02 6246 5475 E rosemary.white-at-csiro.au
for further information.
==============================Original Headers============================== 11, 46 -- From prvs=581137107=Rosemary.White-at-csiro.au Tue May 26 01:35:38 2015 11, 46 -- Received: from act-MTAout5.csiro.au (act-MTAout5.csiro.au [150.229.7.42]) 11, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4Q6ZbLd013114 11, 46 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2015 01:35:37 -0500 11, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 11, 46 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; 11, 46 -- t=1432622138; x=1464158138; 11, 46 -- h=from:to:subject:date:message-id:in-reply-to:content-id: 11, 46 -- content-transfer-encoding:mime-version; 11, 46 -- bh=/rZi0Hxl3alFbcL+yWM7VfIqzUym0BQ/6tacpTXShcQ=; 11, 46 -- b=sCE6/HaysnEgYX6W7wmZ+miBsR0OozIPXB8XsSgd/z/nrJwQaGvwre0N 11, 46 -- Urw91d2qe1FwtTN8D71S2N82LnFOM5rK7FR9XdIU41njPlMxOrcQuq5bP 11, 46 -- oqNyWV+6gPq9Xat; 11, 46 -- X-SBRS: 4.0 11, 46 -- X-IronPort-Anti-Spam-Filtered: true 11, 46 -- X-IronPort-Anti-Spam-Result: A+EZKQDTEmRVjACwBSSwhIATAJKcgCJcgmZ+XgEFxHOENYFAAoFFOxEBAQEBAQEBAw4BAQEnT4MLLAhIIjpRAT5CJQIEExyIEAEMnzizOQEfizqFDBaEFwWQTIZxhlqBKT6DM5IVgQSDF28BAYFEgQEBAQE 11, 46 -- X-IPAS-Result: A+EZKQDTEmRVjACwBSSwhIATAJKcgCJcgmZ+XgEFxHOENYFAAoFFOxEBAQEBAQEBAw4BAQEnT4MLLAhIIjpRAT5CJQIEExyIEAEMnzizOQEfizqFDBaEFwWQTIZxhlqBKT6DM5IVgQSDF28BAYFEgQEBAQE 11, 46 -- X-IronPort-AV: E=Sophos;i="5.13,496,1427720400"; 11, 46 -- d="scan'208";a="57209352" 11, 46 -- Received: from exhtca02-cdc.nexus.csiro.au ([IPv6:2405:b000:601:13::247:22]) 11, 46 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES256-SHA; 26 May 2015 16:35:35 +1000 11, 46 -- Received: from EXHTCA01-CDC.nexus.csiro.au (2405:b000:601:13::247:21) by 11, 46 -- ExHTCA02-CDC.nexus.csiro.au (2405:b000:601:13::247:22) with Microsoft SMTP 11, 46 -- Server (TLS) id 14.3.224.2; Tue, 26 May 2015 16:35:34 +1000 11, 46 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80::9de3:d7af:4ed1:b7b6]) by 11, 46 -- exhtca01-cdc.nexus.csiro.au ([fe80::296a:b134:b85b:89de%13]) with mapi id 11, 46 -- 14.03.0224.002; Tue, 26 May 2015 16:35:34 +1000 11, 46 -- From: {Rosemary.White-at-csiro.au} 11, 46 -- To: {microscopy-at-microscopy.com} 11, 46 -- Subject: Research Scientist, Microscopy at CSIRO, Australia 11, 46 -- Thread-Topic: Research Scientist, Microscopy at CSIRO, Australia 11, 46 -- Thread-Index: AQHQl34rvgyLzCrPYEeVE6Ao/EodOA== 11, 46 -- Date: Tue, 26 May 2015 06:35:34 +0000 11, 46 -- Message-ID: {D18A5051.193674%rosemary.white-at-csiro.au} 11, 46 -- In-Reply-To: {201505260329.t4Q3TYYw022334-at-ns.microscopy.com} 11, 46 -- Accept-Language: en-NZ, en-US, en-AU 11, 46 -- Content-Language: en-US 11, 46 -- X-MS-Has-Attach: 11, 46 -- X-MS-TNEF-Correlator: 11, 46 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 11, 46 -- x-originating-ip: [152.83.195.117] 11, 46 -- Content-Type: text/plain; charset="us-ascii" 11, 46 -- Content-ID: {2CCEA09FB4BFF64981CD8A6F53D3367A-at-csiro.au} 11, 46 -- MIME-Version: 1.0 11, 46 -- Content-Transfer-Encoding: 8bit 11, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4Q6ZbLd013114 ==============================End of - Headers==============================
I would like to thank everyone for advices, they were all valuable. Especially, I would like to thank Harry Verhulst from Ametek BV, who helped me solve the problem. The geometry settings of the system were incorrect and, after the correction of these settings, the quant results are much better.
Kind regards,
Krzysztof Rola
W dniu 2015-05-08 o 15:59, K.Rola pisze: } Dear all, } } I work with FEI Nova NanoSEM 230 equipped with Apollo 40 detector by } EDAX. I have a problem concerning wrong results of EDS quantitative } analysis in a standardless mode. This happens for some of our samples, } such as CaF2, In2O3, CeO2, hydroxyapatite as well as for standards } (from SPI supplies), such as fluorapatite, indium phosphide, etc. I } realize that the standardless analysis method is not an accurate one, } though the differences between real and measured values seem to be too } large (up to about 10%). For example, fluorapatite contains 38,6 wt% } of Ca, while the measured value for Ca is 28,9 wt%. } I tried to find a solution for the problem. I made a calibration using } Cu and Al K lines. I checked HPD, but there was a good fit between } measured and theoretical spectra. I was changing various parameters, } such as working distance, spot size, acceleration voltage, time } constant, but differences between real and measured values were } roughly remaining the same. Next, I tried to use SEC parameters } correction. For example, based on the In2O3 and InP, I set SEC value } for phosphor. Nevertheless, this deteriorated the results of } quantitative analysis for a pentaphosphate. It seems that SEC } correction is not a sufficient solution. } Finally, I tried measurement using our old and rarely used Philips SEM } 515 microscope equipped with SUTW+ detector (also by EDAX) cooled with } liquid nitrogen. Surprisingly, results of quantitative analysis for } hydroxyapatite turned out to be almost correct. This suggests a } problem with the newer Apollo detector. } Does the detector work wrong? Is there any solution to this problem, } which can be found by a user? } } Krzysztof Rola } } Institute of Low Temperature and Structural Research } Polish Academy of Sciences } Wroclaw, Poland }
==============================Original Headers============================== 6, 20 -- From k.rola-at-int.pan.wroc.pl Tue May 26 02:22:34 2015 6, 20 -- Received: from mserv3.int.pan.wroc.pl (mserv3.int.pan.wroc.pl [156.17.85.6]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4Q7MYkI003266 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 26 May 2015 02:22:34 -0500 6, 20 -- Received: from [192.168.25.130] (unknown [192.168.25.130]) 6, 20 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 6, 20 -- (No client certificate requested) 6, 20 -- by mserv3.int.pan.wroc.pl (Postfix) with ESMTPSA id DAC9F4233A 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 26 May 2015 09:22:32 +0200 (CEST) 6, 20 -- Message-ID: {55641F3F.3020003-at-int.pan.wroc.pl} 6, 20 -- Date: Tue, 26 May 2015 09:22:39 +0200 6, 20 -- From: "K.Rola" {k.rola-at-int.pan.wroc.pl} 6, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 6, 20 -- MIME-Version: 1.0 6, 20 -- To: Microscopy-at-Microscopy.Com 6, 20 -- Subject: Re: SEM/EDS - problem with standardless quantitative analysis 6, 20 -- References: {554CC133.7060708-at-int.pan.wroc.pl} 6, 20 -- In-Reply-To: {554CC133.7060708-at-int.pan.wroc.pl} 6, 20 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
They do resemble emptied fungal spores, and there are structures (on the right of the clump, and center of the clump going to the left) that look like sporangial stalks. If this is what they are, then the Mn and Fe is probably from the soil - or, the Mn is really S, which one would expect in a spore wall. Lots of S-containing proteins, especially if (*IF*) this is a) fungal and b) a fungus with keratinous cell walls. But ... I wouldn't go beyond "resembles" on just this image. Should be lots of fungal bits and pieces in your soil sample.
Phil
On 05/22/2015 15:41 , AssociationManagement-at-microscopy.org wrote: } Below is the result of your form, submitted on Friday, May 22, 2015 at 03:41:39 PM. } } realname - Valerie Lecomte } Email - valerie.lecomte-at-usherbrooke.ca } ORGANIZATION - University of Sherbrooke } EDUCATION - Graduate College } LOCATION - Sherbrooke. Qc, Canada } SUBJECT_OF_QUESTION - soil unidentified particle } QUESTION - Dear community members, } } I am doing a research project on metals in humic soils (humus in brunisol and podzol). I look at dried soil samples with a SEM under variable pressure, without coating, and I found a particular structure (see dropbox link bellow). I would like to know if someone can confirm what it is. I am thinking that it could be dessicated fungus. The image is a BSD image and the structure contains mainly carbon and oxygen, but also manganese, iron and calcium. } } Thanks for your help, } Regards, } Valerie } } https://www.dropbox.com/s/1bcvhh1vuwyuxj8/Soil.jpeg?dl=0 } }
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 34 -- From oshel1pe-at-cmich.edu Tue May 26 07:29:45 2015 5, 34 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4QCTjbh008508 5, 34 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2015 07:29:45 -0500 5, 34 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 5, 34 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t4QCTcJ6009818; 5, 34 -- Tue, 26 May 2015 08:29:42 -0400 5, 34 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 5, 34 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 5, 34 -- (TLS) id 14.3.195.1; Tue, 26 May 2015 08:29:39 -0400 5, 34 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 34 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 5, 34 -- 14.3.195.1; Tue, 26 May 2015 08:29:42 -0400 5, 34 -- Message-ID: {55646732.9020200-at-cmich.edu} 5, 34 -- Date: Tue, 26 May 2015 08:29:38 -0400 5, 34 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 34 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 34 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 34 -- MIME-Version: 1.0 5, 34 -- To: Valerie Lecomte {valerie.lecomte-at-usherbrooke.ca} , 5, 34 -- micro 5, 34 -- {microscopy-at-microscopy.com} 5, 34 -- Subject: Re: Ask-A-Microscopist 5, 34 -- References: {25363557.10752.1432323708703.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 34 -- In-Reply-To: {25363557.10752.1432323708703.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 34 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 5, 34 -- Content-Transfer-Encoding: 7bit 5, 34 -- X-Originating-IP: [141.209.2.100] 5, 34 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 34 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 34 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 5, 34 -- X-CanItPRO-Stream: default 5, 34 -- X-Canit-Stats-ID: 02OwAtGha - ff1595abb6eb - 20150526 5, 34 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I'm in the enviable position of replacing our diffusion pumped SEM with a new one (Yay!) which will almost certainly be turbo pumped. Up until this time, the Haskris chiller has served both the SEM with occasional diversion of some of the flow to the vacuum evaporator. My question is, what's the best way to handle this now fairly minor use of the chiller - typically once a month or less? I was thinking of options such as running up both pieces of equipment on a weekly basis, but maybe someone else has come up with a better plan. It seems rather wasteful and possibly harmful to let the chiller run 24/7 with no load, and likewise the evaporator really doesn't need to be on all the time. Also any advice on what additives I should be using to the water would be helpful. Right now I'm using 10% propylene glycol, but don't know if this is the best coolant to be using with occasional use.
Thanks in advance,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
As a reminder - 24 hours remain to register for the workshop "Big, Deep, and Smart Data Analytics in Materials Imaging" organized jointly by the five DOE Nanoscale Science Research Centers (Program Committee: E. Stach, J. Werner, D. Miller, S.V. Kalinin and J. Schuck), to be held at Oak Ridge National Laboratory on June 8-10.
This workshop brings together researchers from different imaging disciplines (electron microscopy, scanning probe microscopy, focused x-ray, neutron, atom probe tomography, chemical imaging, optical microscopies) with the experts in mathematical/statistical/computational approaches to discuss opportunities and future needs in the integration of advanced data analytics and theory into imaging science. It will provide a forum to present achievements in the various imaging disciplines with emphasis on acquisition, visualization, and analysis of multidimensional data sets, the corresponding approaches for theory-experiment matching, and novel opportunities for instrumental development enabled by the availability of high speed data analytic tools.
The program and invited speaker list is now available at http://www.cnms.ornl.gov/JointNSRC2015/.
Looking forward to seeing you at ORNL!
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Theme Leader, Center for Nanophase Materials Science
Oak Ridge National Laboratory
Phone: (865) 241-0236
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We have a BioRad / Polaron E5100 sputter coater with a broken HT cable that needs replacing. Serial number 88107. Cable is approx. 12-15 inches in length and is coaxial. Does anyone have a spare, or know of a supplier for parts?
Thank you
Fred Hayes AMCaT Lab Manager Chem Engr and Mat Sci Univ of CA Davis Davis CA 95616 5300-752-0284
==============================Original Headers============================== 4, 53 -- From fahayes-at-ucdavis.edu Tue May 26 17:38:18 2015 4, 53 -- Received: from smtp3.ucdavis.edu (smtp3.ucdavis.edu [128.120.32.129]) 4, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4QMcHLJ024561 4, 53 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2015 17:38:17 -0500 4, 53 -- Received: from exhub.ex.ad3.ucdavis.edu (exhub.ex.ad3.ucdavis.edu [169.237.229.112]) 4, 53 -- by smtp3.ucdavis.edu (8.14.4/8.14.5/it-oel6-mimedefang-smtp-1.9) with ESMTP id t4QMbnnk054277 4, 53 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=OK) 4, 53 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2015 15:38:16 -0700 4, 53 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (207.46.163.205) 4, 53 -- by exhub.ex.ad3.ucdavis.edu (169.237.229.112) with Microsoft SMTP Server 4, 53 -- (TLS) id 14.3.224.2; Tue, 26 May 2015 15:37:47 -0700 4, 53 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com (10.242.127.155) by 4, 53 -- DM2PR0801MB650.namprd08.prod.outlook.com (10.242.127.153) with Microsoft SMTP 4, 53 -- Server (TLS) id 15.1.172.22; Tue, 26 May 2015 22:37:46 +0000 4, 53 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) by 4, 53 -- DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) with mapi id 4, 53 -- 15.01.0166.017; Tue, 26 May 2015 22:37:46 +0000 4, 53 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 4, 53 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 53 -- Subject: looking for a HT cable for a Polaron E5100 sputter coater 4, 53 -- Thread-Topic: looking for a HT cable for a Polaron E5100 sputter coater 4, 53 -- Thread-Index: AdCYA7y95AF2B6wASoWs4rqZZySfRQ== 4, 53 -- Date: Tue, 26 May 2015 22:37:45 +0000 4, 53 -- Message-ID: {DM2PR0801MB65217B70992A781AA647BC0BECC0-at-DM2PR0801MB652.namprd08.prod.outlook.com} 4, 53 -- Accept-Language: en-US 4, 53 -- Content-Language: en-US 4, 53 -- X-MS-Has-Attach: 4, 53 -- X-MS-TNEF-Correlator: 4, 53 -- authentication-results: spf=none (sender IP is ) 4, 53 -- smtp.mailfrom=fahayes-at-UCDAVIS.EDU; 4, 53 -- x-originating-ip: [169.237.64.26] 4, 53 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB650; 4, 53 -- x-microsoft-antispam-prvs: {DM2PR0801MB650CDB3FDE602B3FDE73A0ABECC0-at-DM2PR0801MB650.namprd08.prod.outlook.com} 4, 53 -- x-exchange-antispam-report-test: UriScan:; 4, 53 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(520003)(5005006)(3002001);SRVR:DM2PR0801MB650;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB650; 4, 53 -- x-forefront-prvs: 0588B2BD96 4, 53 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(189002)(199003)(40224003)(87936001)(5001860100001)(97736004)(5001830100001)(81156007)(4001540100001)(88552001)(90282001)(54356999)(2656002)(99286002)(33656002)(76576001)(189998001)(64706001)(110136002)(5001960100002)(75432002)(107886002)(101416001)(66066001)(105586002)(74316001)(2501003)(122556002)(68736005)(77096005)(102836002)(2351001)(229853001)(89122001)(106356001)(86362001)(450100001)(77156002)(92566002)(62966003)(50986999)(46102003)(2900100001)(40100003)(5002640100001);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR0801MB650;H:DM2PR0801MB652.namprd08.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 4, 53 -- received-spf: None (protection.outlook.com: UCDAVIS.EDU does not designate 4, 53 -- permitted sender hosts) 4, 53 -- Content-Type: text/plain; charset="us-ascii" 4, 53 -- MIME-Version: 1.0 4, 53 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 26 May 2015 22:37:45.6851 4, 53 -- (UTC) 4, 53 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 4, 53 -- X-MS-Exchange-CrossTenant-id: a8046f64-66c0-4f00-9046-c8daf92ff62b 4, 53 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR0801MB650 4, 53 -- X-OriginatorOrg: ucdavis.edu 4, 53 -- X-Greylist: Sender succeeded STARTTLS authentication, not delayed by milter-greylist-4.4.3 (smtp3.ucdavis.edu [128.120.32.8]); Tue, 26 May 2015 15:38:16 -0700 (PDT) 4, 53 -- X-Virus-Scanned: clamav-milter 0.98.1 at smtp3 4, 53 -- X-Virus-Status: Clean 4, 53 -- X-Scanned-By: MIMEDefang 2.74 4, 53 -- Content-Transfer-Encoding: 8bit 4, 53 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4QMcHLJ024561 ==============================End of - Headers==============================
On May 26, 2015, at 7:13 AM, jehrman-at-mta.ca wrote:
} Hi listers, } } I'm in the enviable position of replacing our diffusion pumped SEM } with } a new one (Yay!) which will almost certainly be turbo pumped. Up until } this time, the Haskris chiller has served both the SEM with occasional } diversion of some of the flow to the vacuum evaporator. My question } is, } what's the best way to handle this now fairly minor use of the } chiller - } typically once a month or less? I was thinking of options such as } running up both pieces of equipment on a weekly basis, but maybe } someone } else has come up with a better plan. It seems rather wasteful and } possibly harmful to let the chiller run 24/7 with no load, and } likewise } the evaporator really doesn't need to be on all the time. Also any } advice on what additives I should be using to the water would be } helpful. Right now I'm using 10% propylene glycol, but don't know if } this is the best coolant to be using with occasional use. } } Thanks in advance, } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB E4L 1G7 } CANADA
Dear Jim, Does the Haskris cool the SEM lenses? In this case keeping them at constant temperature makes the scope more stable. When I was in charge of the HVEM, we left the chillers on all the time, and in over 20 years we had no problems. We added an anti-corrosion preparation from Aqua Labs, Aquatreet 42, which was Mo-based, and we kept the pH between 7.5 and 8 to prevent solvation of the Cu tubing. If Aqua Labs cannot supply the product, Skasol has a similar one. (Aqua supplied the East, and Skasol, at least, the West). Yours, Bill
==============================Original Headers============================== 8, 32 -- From wtivol-at-sbcglobal.net Tue May 26 18:28:14 2015 8, 32 -- Received: from nm9-vm7.access.bullet.mail.gq1.yahoo.com (nm9-vm7.access.bullet.mail.gq1.yahoo.com [216.39.63.187]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4QNSDbP013422 8, 32 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2015 18:28:14 -0500 8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1432682893; bh=L0wJ5PDtqF7e60XXTR4ejeoEyBUDCTU+Ynb+Upo7kOY=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=mY4PmS8IuXYEwcVnFh3s+10BqR0+a9Jalhvm0nb9AYhGmFxb+sO2a4NpPeN/DMW50IsXxU2SoxWIhwBZlnNJcRQUAyR6Iz16X2q6CSM9nT+N6g4Mh3tn4dSwXad5koQucvb1FOEl5uhQAD0YAn4zffmMNNApe792MiruMfxUcIv93vEVCqd18ySGR+YAVayA9STjT8eEaPFVU0+RHiMd4s1eox5K7nWb3Q1dE6W4HHX0rqcxZuFLWGODznTK81y1/PjLxC7fzb/a8esiiiOM1LadjpK5LNMq0EvrCGw89GLPDcBvHkM1pBjFI7Cy4eV7KqTlKIu2YSqywaZj/wVzxA== 8, 32 -- Received: from [216.39.60.168] by nm9.access.bullet.mail.gq1.yahoo.com with NNFMP; 26 May 2015 23:28:13 -0000 8, 32 -- Received: from [67.195.22.118] by tm4.access.bullet.mail.gq1.yahoo.com with NNFMP; 26 May 2015 23:28:13 -0000 8, 32 -- Received: from [127.0.0.1] by smtp113.sbc.mail.gq1.yahoo.com with NNFMP; 26 May 2015 23:28:13 -0000 8, 32 -- X-Yahoo-Newman-Id: 446831.36249.bm-at-smtp113.sbc.mail.gq1.yahoo.com 8, 32 -- X-Yahoo-Newman-Property: ymail-3 8, 32 -- X-YMail-OSG: SYX7ecsVM1mgQ0_4pJJIIjqvtYIt.I0uVwiVNKsNeUai95T 8, 32 -- MVC.PDXWYSg60vKLkj6bwgbN1woBpEhwmTo_PmWkOfyaN1s35E4N3w7Ooaia 8, 32 -- YakVNiDkNhkdTxA9FLBRpgswzIdsrl6qz87wsbJ6jGKRGkwQEyEDAUZVmPik 8, 32 -- hOJ.mLDNNvfC6T9wap62b2sNuR0TvBLjQlJiGFtQCay8CqbueJi4c_J2OEEG 8, 32 -- HdFo_WhEKLbW0_VK5KepG4WaJBjhe_LjRx4GedRy32dQcRMSLQ0iMPJxZYUv 8, 32 -- z.eKZ7Z7nuyBtSwq914Kc4UiSAYICeVpv9jHfiIySQ.FBO4QaJoGcltelp6F 8, 32 -- _NQAWC12WFSXM4KKhDQy1.kIJ3ROKNQ9tAbt40k1.Fhfj3LU8K8P9_xDYhR5 8, 32 -- bJB1pYHr.lrznYpQ0ghwGyV7Y0BPL060rt6U4R73qWpZzbzl8Suz4rdvkEqx 8, 32 -- 34ZhzvjRjgxF6vFmxdgA1wilaMYeXfMmkm5RhRGAlbgn9.B2FRk9kt0ZrfNw 8, 32 -- bpCUVK.K7RAMUaLrBWDYyG_F4WFPruoFbKjtEUidGTzA- 8, 32 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 8, 32 -- Message-Id: {844BDCCC-E93B-4EDC-AD96-7F64AABE5E4E-at-sbcglobal.net} 8, 32 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 8, 32 -- To: jehrman-at-mta.ca, microscopy-at-microscopy.com 8, 32 -- In-Reply-To: {201505261413.t4QED1CS014478-at-ns.microscopy.com} 8, 32 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 32 -- Content-Transfer-Encoding: 7bit 8, 32 -- Mime-Version: 1.0 (Apple Message framework v936) 8, 32 -- Subject: Re: [Microscopy] part time chiller use - best strategy? 8, 32 -- Date: Tue, 26 May 2015 16:28:10 -0700 8, 32 -- References: {201505261413.t4QED1CS014478-at-ns.microscopy.com} 8, 32 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: ASU
Title-Subject: [Filtered] mitochondria contrast
Message: we have a few mammalian skeletal muscle samples that are already embedded in SpurrÂs resin. They were conventionally fixed with glutaraldehyde and OsO4, and en bloc stained with uranyl acetate. The mitochondria are poorly contrasted within the muscle fibers using uranyl acetate (50% ethanolic solution) and lead citrate post-staining. Section thickness is 70 nm. Are there any modified post-staining techniques that can preferentially enhance contrast of the mitochondria vs the surrounding muscle fibers?
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Thank you all, Who kindly provided me with valuable advices. I hope we will be able to solve our CM100 problem soon.
My best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
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==============================Original Headers============================== 7, 43 -- From benada-at-biomed.cas.cz Wed May 27 01:53:26 2015 7, 43 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 7, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4R6rPC1032478 7, 43 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 01:53:26 -0500 7, 43 -- X-ASG-Debug-ID: 1432709604-05011e634614000001-4CH8be 7, 43 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id h0ghv6X5RkoMq03X (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 08:53:24 +0200 (CEST) 7, 43 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 7, 43 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 7, 43 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) 7, 43 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 43 -- (No client certificate requested) 7, 43 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 9D3E22C8075 7, 43 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 08:53:19 +0200 (CEST) 7, 43 -- Date: Wed, 27 May 2015 08:53:15 +0200 7, 43 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 7, 43 -- To: {microscopy-at-microscopy.com} 7, 43 -- Subject: Re: [Microscopy] Philips CM100 trouble 7, 43 -- Message-ID: {20150527085315.01b56dbf-at-u117fs} 7, 43 -- X-ASG-Orig-Subj: Re: [Microscopy] Philips CM100 trouble 7, 43 -- In-Reply-To: {CALbJ0i4e1QjEcet_fNY7xFZEKgPpjqXAfqgZKy9mv+64G0zyqg-at-mail.gmail.com} 7, 43 -- References: {201505250841.t4P8fhIv018828-at-ns.microscopy.com} 7, 43 -- {CALbJ0i4e1QjEcet_fNY7xFZEKgPpjqXAfqgZKy9mv+64G0zyqg-at-mail.gmail.com} 7, 43 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 7, 43 -- =?UTF-8?B?xIxS?= 7, 43 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 7, 43 -- MIME-Version: 1.0 7, 43 -- Content-Type: text/plain; charset=US-ASCII 7, 43 -- Content-Transfer-Encoding: 7bit 7, 43 -- X-IoP-CAS-MailScanner-ID: 9D3E22C8075.254AD 7, 43 -- X-IoP-CAS-MailScanner: Processed 7, 43 -- X-Spam-Status: No 7, 43 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 7, 43 -- X-Barracuda-Start-Time: 1432709604 7, 43 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 7, 43 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 7, 43 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 7, 43 -- X-Barracuda-BRTS-Status: 1 7, 43 -- X-Barracuda-Spam-Score: 0.00 7, 43 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 7, 43 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.19315 7, 43 -- Rule breakdown below 7, 43 -- pts rule name description 7, 43 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
Hi Fred, Did you try to contact Quorum Technologies? They have E5100 on the list of partially supported devices. {http://www.quorumtech.com/customer-support/product-and-spares-supported.html}
I hope they could help you.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
tel.: 241062399
On Tue, 26 May 2015 17:44:08 -0500, fahayes-at-ucdavis.edu wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have a BioRad / Polaron E5100 sputter coater with a broken HT } cable that needs replacing. Serial number 88107. Cable is approx. } 12-15 inches in length and is coaxial. Does anyone have a spare, or } know of a supplier for parts? } } Thank you } } Fred Hayes } AMCaT Lab Manager } Chem Engr and Mat Sci } Univ of CA Davis } Davis CA 95616 } 5300-752-0284 } } } ==============================Original } Headers============================== 4, 53 -- From } fahayes-at-ucdavis.edu Tue May 26 17:38:18 2015 4, 53 -- Received: from } smtp3.ucdavis.edu (smtp3.ucdavis.edu [128.120.32.129]) 4, 53 -- } by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } t4QMcHLJ024561 4, 53 -- for {Microscopy-at-microscopy.com} ; Tue, } 26 May 2015 17:38:17 -0500 4, 53 -- Received: from } exhub.ex.ad3.ucdavis.edu (exhub.ex.ad3.ucdavis.edu [169.237.229.112]) } 4, 53 -- by smtp3.ucdavis.edu } (8.14.4/8.14.5/it-oel6-mimedefang-smtp-1.9) with ESMTP id } t4QMbnnk054277 4, 53 -- (version=TLSv1/SSLv3 } cipher=AES128-SHA bits=128 verify=OK) 4, 53 -- for } {Microscopy-at-microscopy.com} ; Tue, 26 May 2015 15:38:16 -0700 4, 53 -- } Received: from na01-bl2-obe.outbound.protection.outlook.com } (207.46.163.205) 4, 53 -- by exhub.ex.ad3.ucdavis.edu } (169.237.229.112) with Microsoft SMTP Server 4, 53 -- (TLS) id } 14.3.224.2; Tue, 26 May 2015 15:37:47 -0700 4, 53 -- Received: from } DM2PR0801MB652.namprd08.prod.outlook.com (10.242.127.155) by 4, 53 } -- DM2PR0801MB650.namprd08.prod.outlook.com (10.242.127.153) with } Microsoft SMTP 4, 53 -- Server (TLS) id 15.1.172.22; Tue, 26 May } 2015 22:37:46 +0000 4, 53 -- Received: from } DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) by 4, 53 } -- DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) with } mapi id 4, 53 -- 15.01.0166.017; Tue, 26 May 2015 22:37:46 +0000 4, } 53 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 4, 53 -- To: } "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 53 -- } Subject: looking for a HT cable for a Polaron E5100 sputter coater 4, } 53 -- Thread-Topic: looking for a HT cable for a Polaron E5100 } sputter coater 4, 53 -- Thread-Index: } AdCYA7y95AF2B6wASoWs4rqZZySfRQ== 4, 53 -- Date: Tue, 26 May 2015 } 22:37:45 +0000 4, 53 -- Message-ID: } {DM2PR0801MB65217B70992A781AA647BC0BECC0-at-DM2PR0801MB652.namprd08.prod.outlook.com} } 4, 53 -- Accept-Language: en-US 4, 53 -- Content-Language: en-US 4, } 53 -- X-MS-Has-Attach: 4, 53 -- X-MS-TNEF-Correlator: 4, 53 -- } authentication-results: spf=none (sender IP is ) 4, 53 -- } smtp.mailfrom=fahayes-at-UCDAVIS.EDU; 4, 53 -- x-originating-ip: } [169.237.64.26] 4, 53 -- x-microsoft-antispam: } UriScan:;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB650; 4, 53 -- } x-microsoft-antispam-prvs: } {DM2PR0801MB650CDB3FDE602B3FDE73A0ABECC0-at-DM2PR0801MB650.namprd08.prod.outlook.com} } 4, 53 -- x-exchange-antispam-report-test: UriScan:; 4, 53 -- } x-exchange-antispam-report-cfa-test: } BCL:0;PCL:0;RULEID:(601004)(520003)(5005006)(3002001);SRVR:DM2PR0801MB650;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB650; } 4, 53 -- x-forefront-prvs: 0588B2BD96 4, 53 -- } x-forefront-antispam-report: } SFV:NSPM;SFS:(10009020)(6009001)(189002)(199003)(40224003)(87936001)(5001860100001)(97736004)(5001830100001)(81156007)(4001540100001)(88552001)(90282001)(54356999)(2656002)(99286002)(33656002)(76576001)(189998001)(64706001)(110136002)(5001960100002)(75432002)(107886002)(101416001)(66066001)(105586002)(74316001)(2501003)(122556002)(68736005)(77096005)(102836002)(2351001)(229853001)(89122001)(106356001)(86362001)(450100001)(77156002)(92566002)(62966003)(50986999)(46102003)(2900100001)(40100003)(5002640100001);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR0801MB650;H:DM2PR0801MB652.namprd08.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; } 4, 53 -- received-spf: None (protection.outlook.com: UCDAVIS.EDU does } not designate 4, 53 -- permitted sender hosts) 4, 53 -- } Content-Type: text/plain; charset="us-ascii" 4, 53 -- MIME-Version: } 1.0 4, 53 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 26 May } 2015 22:37:45.6851 4, 53 -- (UTC) 4, 53 -- } X-MS-Exchange-CrossTenant-fromentityheader: Hosted 4, 53 -- } X-MS-Exchange-CrossTenant-id: a8046f64-66c0-4f00-9046-c8daf92ff62b 4, } 53 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: } DM2PR0801MB650 4, 53 -- X-OriginatorOrg: ucdavis.edu 4, 53 -- } X-Greylist: Sender succeeded STARTTLS authentication, not delayed by } milter-greylist-4.4.3 (smtp3.ucdavis.edu [128.120.32.8]); Tue, 26 May } 2015 15:38:16 -0700 (PDT) 4, 53 -- X-Virus-Scanned: clamav-milter } 0.98.1 at smtp3 4, 53 -- X-Virus-Status: Clean 4, 53 -- X-Scanned-By: } MIMEDefang 2.74 4, 53 -- Content-Transfer-Encoding: 8bit 4, 53 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id t4QMcHLJ024561 ==============================End } of - Headers==============================
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==============================Original Headers============================== 10, 45 -- From benada-at-biomed.cas.cz Wed May 27 02:09:52 2015 10, 45 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 10, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4R79oI6019481 10, 45 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 02:09:51 -0500 10, 45 -- X-ASG-Debug-ID: 1432710590-05011e6345149a0001-4CH8be 10, 45 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id 1wB5FffUquoMBzQd (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 09:09:50 +0200 (CEST) 10, 45 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 10, 45 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 10, 45 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) 10, 45 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 10, 45 -- (No client certificate requested) 10, 45 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id D3FD52C80EC 10, 45 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 09:09:46 +0200 (CEST) 10, 45 -- Date: Wed, 27 May 2015 09:09:45 +0200 10, 45 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 45 -- To: {microscopy-at-microscopy.com} 10, 45 -- Subject: Re: [Microscopy] looking for a HT cable for a Polaron E5100 sputter 10, 45 -- coater 10, 45 -- Message-ID: {20150527090945.14236442-at-u117fs} 10, 45 -- X-ASG-Orig-Subj: Re: [Microscopy] looking for a HT cable for a Polaron E5100 sputter 10, 45 -- coater 10, 45 -- In-Reply-To: {201505262244.t4QMi8JK027477-at-ns.microscopy.com} 10, 45 -- References: {201505262244.t4QMi8JK027477-at-ns.microscopy.com} 10, 45 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 45 -- =?UTF-8?B?xIxS?= 10, 45 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 10, 45 -- MIME-Version: 1.0 10, 45 -- Content-Type: text/plain; charset=US-ASCII 10, 45 -- Content-Transfer-Encoding: 7bit 10, 45 -- X-IoP-CAS-MailScanner-ID: D3FD52C80EC.685E0 10, 45 -- X-IoP-CAS-MailScanner: Processed 10, 45 -- X-Spam-Status: No 10, 45 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 10, 45 -- X-Barracuda-Start-Time: 1432710590 10, 45 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 10, 45 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 10, 45 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 10, 45 -- X-Barracuda-BRTS-Status: 1 10, 45 -- X-Barracuda-Spam-Score: 0.50 10, 45 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M 10, 45 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.19315 10, 45 -- Rule breakdown below 10, 45 -- pts rule name description 10, 45 -- ---- ---------------------- -------------------------------------------------- 10, 45 -- 0.50 BSF_RULE7568M Custom Rule 7568M ==============================End of - Headers==============================
David, You can try triple staining with 1% filtered and aq. Tannic acid (10 min) before the UA and Pb. The grids must be formvar coated as the copper will react with the TA.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, May 26, 2015 11:51 PM To: delannoy-at-jhmi.edu
X-from: dlowry-at-asu.edu
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: ASU
Title-Subject: [Filtered] mitochondria contrast
Message: we have a few mammalian skeletal muscle samples that are already embedded in SpurrÂs resin. They were conventionally fixed with glutaraldehyde and OsO4, and en bloc stained with uranyl acetate. The mitochondria are poorly contrasted within the muscle fibers using uranyl acetate (50% ethanolic solution) and lead citrate post-staining. Section thickness is 70 nm. Are there any modified post-staining techniques that can preferentially enhance contrast of the mitochondria vs the surrounding muscle fibers?
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue May 26 22:39:23 2015 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4R3dNwO008419 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2015 22:39:23 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id F28CB52F8CA 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2015 22:39:22 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at znl.com 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id 1Ja7YD9mTlfc for {microscopy-at-microscopy.com} ; 14, 28 -- Tue, 26 May 2015 22:39:14 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 6BC2C52F8B4 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2015 22:39:14 -0500 (CDT) 14, 28 -- Message-ID: {55653C62.2090500-at-microscopy.com} 14, 28 -- Date: Tue, 26 May 2015 22:39:14 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:mitochondria contrast 14, 28 -- References: {201505262139.t4QLdWf0023261-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201505262139.t4QLdWf0023261-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201505262139.t4QLdWf0023261-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 14, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 24, 23 -- From prvs=582b1d32e=mdelann1-at-jhmi.edu Wed May 27 08:41:25 2015 24, 23 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [128.220.229.147]) 24, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4RDfPYd025790 24, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2015 08:41:25 -0500 24, 23 -- X-IronPort-AV: E=Sophos;i="5.13,505,1427774400"; 24, 23 -- d="scan'208";a="111833832" 24, 23 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96]) 24, 23 -- by IPMTW1.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 27 May 2015 09:41:25 -0400 24, 23 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 24, 23 -- To: {Microscopy-at-microscopy.com} 24, 23 -- References: {201505270351.t4R3pTjb015228-at-ns.microscopy.com} 24, 23 -- In-Reply-To: {201505270351.t4R3pTjb015228-at-ns.microscopy.com} 24, 23 -- Subject: RE: [Microscopy] viaWWW:mitochondria contrast 24, 23 -- Date: Wed, 27 May 2015 09:41:27 -0400 24, 23 -- Message-ID: {003301d09882$d4894240$7d9bc6c0$-at-jhmi.edu} 24, 23 -- MIME-Version: 1.0 24, 23 -- Content-Type: text/plain; 24, 23 -- charset="iso-8859-1" 24, 23 -- X-Mailer: Microsoft Outlook 14.0 24, 23 -- Thread-Index: AQFeR5uE50mWzj6CFkLHhB0hbUGeP550dj4w 24, 23 -- Content-Language: en-us 24, 23 -- Content-Transfer-Encoding: 8bit 24, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4RDfPYd025790 ==============================End of - Headers==============================
From advertise.bz222p-at-gmail.com Wed May 27 13:47:05 2015 Return-Path: {advertise.bz222p-at-gmail.com} Received: from gmail.com ([202.130.88.148]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t4RIl0b6022878 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 27 May 2015 13:47:01 -0500 Message-ID: {AE0B17D4.ECAF30A5-at-gmail.com}
X-from: schauflingerm-at-missouri.edu
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Email: schauflingerm-at-missouri.edu Name: Martin
Organization: UM
Title-Subject: [Filtered] anti-GFP antibody for IEM
Message: Hello everyone.
I am looking for an anti-GFP antibody that works for both pre- and postembedding labeling approaches. My samples are GFP expressing E. coli. Ideally the antibody should work on FA/GA fixed samples. I'd appreciate any information about antibodies (that are still available!) that have been successfully used on LR-Gold, HM20, or similarly embedded samples.
Thanks!
Martin
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed May 27 17:21:21 2015 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4RMLL54030010 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 17:21:21 -0500 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id 7445B53A28B 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 17:21:21 -0500 (CDT) 15, 28 -- X-Virus-Scanned: amavisd-new at znl.com 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id Ox9JbrBOAdNk for {microscopy-at-microscopy.com} ; 15, 28 -- Wed, 27 May 2015 17:21:07 -0500 (CDT) 15, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 3EDD353A277 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 17:21:07 -0500 (CDT) 15, 28 -- Message-ID: {55664352.7030204-at-microscopy.com} 15, 28 -- Date: Wed, 27 May 2015 17:21:06 -0500 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:anti-GFP antibody for IEM 15, 28 -- References: {201505272057.t4RKvOse027286-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201505272057.t4RKvOse027286-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201505272057.t4RKvOse027286-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear colleagues, I found unknown EM-related objects in the TEM room and wanted to ask if you can help me identify them. We have a technai G20 and I suppose these pieces belong to it. I am no expert in picture sharing through external sites, so let's give it a try:
Dear Stephane, your first image might show - when I look at my Philips EM420 gun section - a top cover on the upper column and IGP valve section to keep it under vacuum during transport. The second image is an adapter piece for the bottom mount flange on the Tecnai or something else belonging to a FEI / Philips SEM. My proposal would be: keep them...
Best wishes, Stefan
Am 28.05.2015 um 13:00 schrieb nizets2-at-yahoo.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues, } I found unknown EM-related objects in the TEM room and wanted to ask if you can help me identify them. } We have a technai G20 and I suppose these pieces belong to it. } I am no expert in picture sharing through external sites, so let's give it a try: } } https://www.flickr.com/gp/24377807-at-N02/S232aH } https://www.flickr.com/gp/24377807-at-N02/j85kC9 } } Hope the links work. } Thanks! } } Stephane } } ==============================Original Headers============================== } 4, 28 -- From nizets2-at-yahoo.com Thu May 28 05:52:42 2015 } 4, 28 -- Received: from nm18-vm0.bullet.mail.bf1.yahoo.com (nm18-vm0.bullet.mail.bf1.yahoo.com [98.139.213.138]) } 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4SAqgug001802 } 4, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2015 05:52:42 -0500 } 4, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1432810362; bh=0T+e3G4OtwpsI+0tJ95rTiKw/nWFqsP7qId2HZljdwE=; h=Date:From:Reply-To:To:Subject:From:Subject; b=uL0LGLV7C7dEKJWj+UtzZ7HUkt4tI6FE7T5R1xFY4C7EdbOkHmB7lqjAO5Qbp8Wokw0ZUA6qT/C0Y23gscHXQZmEhP6CmYB3V88TuhIcQM3fAFCUqR7Ls93qvtECKv0V/HzA91KeFEzL4Nzbu/4jCkGUdbPTZ00884ia9ec1LAEDXJfHlglTZqbiScZ81XhPjXXWi/Fn1hTLzLjma2Nsf4yJLyCUrdrNYhILuKVrVUqwTJodwVRCIhn2GIU6olnfLbWsc7K35OnlJWjMYerN36suWnTb21oarlsXyqeaIqmd0NbbmVSngwtuKNpVv+FfXiin1ZgucLXy+1AkCIMazQ== } 4, 28 -- Received: from [98.139.170.180] by nm18.bullet.mail.bf1.yahoo.com with NNFMP; 28 May 2015 10:52:42 -0000 } 4, 28 -- Received: from [98.139.215.228] by tm23.bullet.mail.bf1.yahoo.com with NNFMP; 28 May 2015 10:52:42 -0000 } 4, 28 -- Received: from [127.0.0.1] by omp1068.mail.bf1.yahoo.com with NNFMP; 28 May 2015 10:52:42 -0000 } 4, 28 -- X-Yahoo-Newman-Property: ymail-3 } 4, 28 -- X-Yahoo-Newman-Id: 324302.27668.bm-at-omp1068.mail.bf1.yahoo.com } 4, 28 -- X-YMail-OSG: GImrSFEVM1lT4fzWZ7o0o74gh8sstUkKHDVg1xCa22KF.ExoE9N14fwkYycyl6Z } 4, 28 -- m8exJvvbz1kwg5VRmpkhVFtjzWe6gzi018Pdy2xeuWgNkrdanxZOKiaPqOPaHeSxUb821ahapJn0 } 4, 28 -- j8jkFGX63jVw6zF5tkqyAiyB2yHClI13oMUVA0iiAYwrFAXA3qfYKr9ESpkW8bxvJwahbJhHPrhL } 4, 28 -- ibYNmAOTtDIVLp8YSav18rAbAdvljLji71Ipm5UkzP1gGHPJCqwHBHlW__LrH6grg.jugIywY.ty } 4, 28 -- 1ewujsk7Geiz0h2MUZE0N11iCHXZaVd75C.ZoeNnbvvu59mT1ADCI.pbL6r6l29FFJi5NA6ENt8e } 4, 28 -- EXh0hbwDeqTsIsJFVJGDqPWb_GPOzm_siyR5ARjIBtcJOr8vfB__i1wHByw8Xf_ShLNQu2GACXJV } 4, 28 -- 5xy7BdMHyT_dHf8SrrJ58EXF1ypAiNBTspFcxoEW38mnbdzAt8nsDonpU7w.0Lt3v2cUHwPG_eea } 4, 28 -- zL.n8yCQ- } 4, 28 -- Received: by 66.196.80.126; Thu, 28 May 2015 10:52:41 +0000 } 4, 28 -- Date: Thu, 28 May 2015 10:52:41 +0000 (UTC) } 4, 28 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 4, 28 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } 4, 28 -- To: Microscopy Listserver {microscopy-at-microscopy.com} } 4, 28 -- Message-ID: {845131800.342119.1432810361044.JavaMail.yahoo-at-mail.yahoo.com} } 4, 28 -- Subject: UEMO } 4, 28 -- MIME-Version: 1.0 } 4, 28 -- Content-Type: text/plain; charset=UTF-8 } 4, 28 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: gary-at-microtechnics.com Name: Dr. Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] SEM documents
Message: Hi all:
I have the following docs that need a new home other than the landfill or shredder:
1. Hitachi S-2700 operator manual and schematics 2. AMR 1000 service manual and brochures 3. PGT IMIX tutorial Version 7 4. CAM SCAN Series 4 brochure 5. ISI Topcon DS-130, DS-130F and WB-6 brochures 6. Polaron E5200 manual with schematics 7. Hitachi S-570 manuals, schematics, all documentation 8. Two (2) Amray 1800 series schematics, PCB layouts #118-163
Please contact me off-line for shipping costs that I'd like reimbursed. The brochures are on me. The manuals are not light weight.
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From benny0439655322345345uky-at-gmail.com Thu May 28 18:00:16 2015 Return-Path: {benny0439655322345345uky-at-gmail.com} Received: from gmail.com ([27.255.72.80]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t4SN0DH7020761 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 28 May 2015 18:00:15 -0500 Message-ID: {1FE48328.4EB5F4AE-at-gmail.com}
X-from: chris.guerin-at-irc.vib-ugent.be
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Email: chris.guerin-at-irc.vib-ugent.be Name: Christopher Guerin
Organization: VIB Bio Imaging COre
Title-Subject: [Filtered] Post doc opening in Ghent/Beerse Belgium
Message: Postdoc Position at VIB Bio Imaging
Description
A 2 year post-doctoral fellowship is available immediately at the VIB Bio Imaging Core (http://bio-imaging-core.be/) in Ghent Belgium. This position is designed for a person who will work on a joint project with Janssen Pharmaceutial Group of Companies (J&J) in Beerse, Belgium and the successful candidate will spend most of their time on the Beerse site.
The project will involve the development of high throughput microscopy for automated imaging of mouse brain slices and analysis of phenotypic changes in models of neurodegenerative disease. Additional partners will provide engineering and computational image analysis expertise. The fellow will be responsible for all aspects of the microscopy and imaging, but will be expected to work closely together with the computational scientist to explore and validate the image and data analyses.
Profile
You have a doctoral degree in biological sciences you have some experience of microscopy experience in or knowledge of mouse neuroanatomy are a plus recent graduates are welcome to apply you are an interactive individual fluency in English is required We offer:
a renewable 2-year contract a competitive salary a position at the interface between industry and the academic world experience in translational research excellent skills and training courses The post will remain open until filled and preference will be given to candidates that can begin before end of June 2015.
Interested candidates should apply through the VIB JOBS webpage http://www.vib.be/en/jobs/Pages/Postdoc-Position-at-VIB-Bio-Imaging.aspx
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri May 29 08:18:39 2015 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4TDIdMu008537 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2015 08:18:39 -0500 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 0D4CE550189 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2015 08:18:39 -0500 (CDT) 19, 28 -- X-Virus-Scanned: amavisd-new at znl.com 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id fx7QJ4sM6moi for {microscopy-at-microscopy.com} ; 19, 28 -- Fri, 29 May 2015 08:18:26 -0500 (CDT) 19, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 4AC3B550180 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2015 08:18:26 -0500 (CDT) 19, 28 -- Message-ID: {55686721.8060908-at-microscopy.com} 19, 28 -- Date: Fri, 29 May 2015 08:18:25 -0500 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:Post doc opening in Ghent/Beerse Belgium 19, 28 -- References: {201505290945.t4T9jijT003277-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201505290945.t4T9jijT003277-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201505290945.t4T9jijT003277-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
2015 Workshop on Microwave Tissue Processing for EM
Date: June 24-26 Location: Madison Area Technical College, Madison, WI Information & Agenda: http://www.tedpella.com/company_html/WorkshopAnnouncement_L5.htm
Presenters: Cindy Smith Microwave Product Manager, Ted Pella Inc. - 530-243-2200 x259 Michael Kostrna Director, MATC EM Lab, Madison Area Technical College - 608-243-4309 Mark Sanders Program Director, University Imaging Center, University of Minnesota 612-624-7938
Explore the ease and efficiency of tissue processing with the PELCO BioWaveŽ Pro. Hands-on techniques will address electron microscopy and immunolabeling in the microwave environment. Basic fundamentals and troubleshooting will be covered as well as novel approaches unique to the PELCO BioWaveŽ Pro.
Mike Toalson VP Sales & Marketing Ted Pella, Inc. PO Box 492477 Redding, CA 96049 Tel: 530-243-2200 x205 Mob: 530-356-5921 mike_toalson-at-tedpella.com http://www.tedpella.com
==============================Original Headers============================== 9, 20 -- From mike_toalson-at-tedpella.com Fri May 29 17:36:34 2015 9, 20 -- Received: from asmx01.boltonsmith.com (asmx01.boltonsmith.com [69.90.54.3]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4TMaY2u030724 9, 20 -- for {Microscopy-at-Microscopy.com} ; Fri, 29 May 2015 17:36:34 -0500 9, 20 -- X-Virus-Scanned: by SpamTitan at boltonsmith.net 9, 20 -- Received: from pellavp205b [12.7.209.242] by intown.net with ESMTP 9, 20 -- (SMTPD-12.0.0.384) id d6a8001758d95823; Fri, 29 May 2015 18:36:29 -0400 9, 20 -- From: "Mike Toalson" {mike_toalson-at-tedpella.com} 9, 20 -- To: {Microscopy-at-Microscopy.com} 9, 20 -- Subject: MATC Workshop on Microwave Tissue Processing for EM 9, 20 -- Date: Fri, 29 May 2015 15:36:32 -0700 9, 20 -- Message-ID: {036d01d09a5f$ea1db350$be5919f0$-at-tedpella.com} 9, 20 -- MIME-Version: 1.0 9, 20 -- Content-Type: text/plain; 9, 20 -- charset="iso-8859-1" 9, 20 -- X-Mailer: Microsoft Outlook 14.0 9, 20 -- Thread-Index: AdCaX8hHTRr03e6FSLaIV/mSPa8jPw== 9, 20 -- Content-Language: en-us 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4TMaY2u030724 ==============================End of - Headers==============================
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I am looking for recommendations for a diamond wire saw to cut oriented single crystals of non- conducting alloys and ceramics. My requirements are: table top style; preferably can accept SouthBay model 260 3-axis goniometers (~4Âx4Âx4Â) or has available 3-axis goniometer accessory; can cut up to 1Â diameter or larger (this size of sample mounted to a 3-axis goniometer); reliable; easy to use; accurate, reproducible cut.
I have considered SouthBay, Well Diamond Saws, MTI and Precision Wire Saw models but am not finding reviews. I would like to know from end users what they think about these saws before I buy one. Thanks in advance!
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat May 30 07:32:24 2015 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4UCWOPX009110 14, 28 -- for {microscopy-at-microscopy.com} ; Sat, 30 May 2015 07:32:24 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id A444855D431 14, 28 -- for {microscopy-at-microscopy.com} ; Sat, 30 May 2015 07:32:24 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at znl.com 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id glAdQ1uTwBZT for {microscopy-at-microscopy.com} ; 14, 28 -- Sat, 30 May 2015 07:32:12 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 65BB855D41C 14, 28 -- for {microscopy-at-microscopy.com} ; Sat, 30 May 2015 07:32:11 -0500 (CDT) 14, 28 -- Message-ID: {5569AD87.5060303-at-microscopy.com} 14, 28 -- Date: Sat, 30 May 2015 07:31:03 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:TEM, SEM sample cutting 14, 28 -- References: {201505291331.t4TDVUOe023406-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201505291331.t4TDVUOe023406-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201505291331.t4TDVUOe023406-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 14, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Organization: University of Arkansas at Little Rock
Title-Subject: [Filtered] Preparation of yeast cells for SEM observation
Message: Dear Listers,
I have been asked by a biology faculty to analyze his yeast cell samples by our SEM (JEOL 7000F, no LV mode) to find out how much Cd atoms his cells have absorbed (by EDS).
I Googled sample preparation methods for yeast cells, and it seems difficult or complicated. If any of you is an expert in this, please help us out by sending your protocol (or guide me to the reference).
I've found this ref, but it says this is for LV mode: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121316/
Best regards,
Fumiya
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat May 30 07:32:27 2015 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4UCWRb8009116 18, 28 -- for {microscopy-at-microscopy.com} ; Sat, 30 May 2015 07:32:27 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id 1B20155D438 18, 28 -- for {microscopy-at-microscopy.com} ; Sat, 30 May 2015 07:32:27 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at znl.com 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id tEv2RFN-wHqw for {microscopy-at-microscopy.com} ; 18, 28 -- Sat, 30 May 2015 07:32:16 -0500 (CDT) 18, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id D50F055D420 18, 28 -- for {microscopy-at-microscopy.com} ; Sat, 30 May 2015 07:32:16 -0500 (CDT) 18, 28 -- Message-ID: {5569ADD0.20701-at-microscopy.com} 18, 28 -- Date: Sat, 30 May 2015 07:32:16 -0500 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: rviaWWW:Preparation of yeast cells for SEM observation 18, 28 -- References: {201505300249.t4U2n4bN023106-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201505300249.t4U2n4bN023106-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201505300249.t4U2n4bN023106-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have particularly good experience with Well Wire Saws (http://www.welldiamondwiresaws.com/index.html). I have used these saw for years and was able to cut ceramics very precisely and reproducibly. Look at the accessories for goniometers and microscopes.
This is really the only type of diamond wire saw I have worked with, so I have no comparative data. Also, I do not have any financial interest in the company! Just consider it a recommendation.
Good luck with your search! Klaus can Benthem
Sent from one of my iThings Please excuse any tipos or erors
==============================Original Headers============================== 4, 36 -- From benthem-at-ucdavis.edu Sat May 30 08:28:26 2015 4, 36 -- Received: from mail-pa0-f46.google.com (mail-pa0-f46.google.com [209.85.220.46]) 4, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4UDSQ60019208 4, 36 -- for {Microscopy-at-microscopy.com} ; Sat, 30 May 2015 08:28:26 -0500 4, 36 -- Received: by pabru16 with SMTP id ru16so78790742pab.1 4, 36 -- for {Microscopy-at-microscopy.com} ; Sat, 30 May 2015 06:28:25 -0700 (PDT) 4, 36 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 36 -- d=1e100.net; s=20130820; 4, 36 -- h=x-gm-message-state:from:content-type:content-transfer-encoding 4, 36 -- :mime-version:subject:message-id:date:to; 4, 36 -- bh=DCU0KkfVUccdWv13Own38dMwEjbiVwBbdKbwZjBaF4M=; 4, 36 -- b=dMMFzkJ/suaENEcPLLGdLAA5wvs3QctwEDowcd+0q56qrZKqoXMTqZcBlL2qQV6oJy 4, 36 -- SpRoxqqBkKzsxqrC8gM/hQ2JdVM158eqOJJZM9ywBWIdNGfuklZs9Cr8n/xnpxYL6xqN 4, 36 -- klNl5QSfFQHF7bp6JvwzJPOlxTJYi/PY8VhpOYahTLU93unXo9Z01APg3EHnOi3UeYZz 4, 36 -- bUXJRZhkNe3mUKraAnjik6v0Eug6UrXzFVGFmqnKJTy+My1h36VsFr110eoyAmNb9zlD 4, 36 -- JRtOuMfJDE7bYRsvGUatvCUHCRZvMF25jOlmnHugdpK7fPbIb8reVbiIa9aKVZegihdV 4, 36 -- dKrw== 4, 36 -- X-Gm-Message-State: ALoCoQlo6P7e0rlgubU/GBN0UOTXbVWpq1ASSVp4X4X2ixvHqrwIGHVwswSjiym6mcbYc7+eYV7c 4, 36 -- X-Received: by 10.67.4.161 with SMTP id cf1mr23873353pad.35.1432992505719; 4, 36 -- Sat, 30 May 2015 06:28:25 -0700 (PDT) 4, 36 -- Received: from [192.168.15.167] ([50.13.190.109]) 4, 36 -- by mx.google.com with ESMTPSA id zs5sm8791633pac.11.2015.05.30.06.28.23 4, 36 -- for {Microscopy-at-microscopy.com} 4, 36 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 4, 36 -- Sat, 30 May 2015 06:28:24 -0700 (PDT) 4, 36 -- From: Klaus van Benthem {benthem-at-ucdavis.edu} 4, 36 -- Content-Type: text/plain; 4, 36 -- charset=us-ascii 4, 36 -- Mime-Version: 1.0 (1.0) 4, 36 -- Subject: TEM, SEM sample cutting 4, 36 -- Message-Id: {5C0BB045-8700-4B18-923C-43655F223C82-at-ucdavis.edu} 4, 36 -- Date: Sat, 30 May 2015 06:28:21 -0700 4, 36 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 4, 36 -- X-Mailer: iPhone Mail (11D257) 4, 36 -- Content-Transfer-Encoding: 8bit 4, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t4UDSQ60019208 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dswxyiu-at-gmail.com Sat May 30 17:38:35 2015 Return-Path: {mike.sfsd4f564s6df45dswxyiu-at-gmail.com} Received: from gmail.com ([175.119.156.122]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t4UMcWsb017168 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 30 May 2015 17:38:34 -0500 Message-ID: {1C78AFEE.459FF0A5-at-gmail.com}
~You Are Invited~
When: July 13-14, 2015 Where: Signal Hill,CA What: Advanced AFM Operation Techniques â Workshop
It's relatively easy to measure images of samples with dimensions of 50 nm (requiring a magnification of 10,000X) or greater with an atomic force microscope (AFM). However, it's often necessary to measure images with features as small as 1 nm (requiring a magnification of 1,000,000X). Measuring images with such high magnification requires specialized skill in the operation of an AFM.
This two-day advanced operator course is designed to help attendees learn the skills required for measuring high-resolution AFM images and is led by AFM experts Peter Eaton, Ph.D. And Paul West, Ph.D. Four samples are scanned to assure specific skills are mastered. The four samples and their associated skills are as follows: 1. Silicon test pattern - calibrating the AFM,basic operation skills; 2. Silicon Substrate â measuring noise floor; 3. Nanoparticles â probe approach; 4. BOPP polymer sample â controlling probe-sample forces.
The course mixes lecture with labwork on atomic force microscopy operation. While we will utilize AFMs from AFMWorkshop to teach basic concepts and demonstrate AFM operation, attendees with experience on any make or model of AFM instrument will find the labwork relevant and practical.
For more information, please visit: http://www.afmworkshop.com/advanced-afm-operation-techniques.html
In my hands the best commercially available anti-GFP antibody is the # 70R-GG001, Fitzgerald Industries International, which I used a lot on yeast http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113337/ The Abcam290 worked nearly as well. Your real problem will be your prep. GFP is sensitive to GA and complete dehydration. All my labeling attempts with a bunch of antibodies failed if the FS had more than 0.5% GA or if the specimen was completely dehydrated. Read the discussion in the paper and contact me if you need more details.
Best, Chris
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Email: schauflingerm-at-missouri.edu Name: Martin
Organization: UM
Title-Subject: [Filtered] anti-GFP antibody for IEM
Message: Hello everyone.
I am looking for an anti-GFP antibody that works for both pre- and postembedding labeling approaches. My samples are GFP expressing E. coli. Ideally the antibody should work on FA/GA fixed samples. I'd appreciate any information about antibodies (that are still available!) that have been successfully used on LR-Gold, HM20, or similarly embedded samples.
Thanks!
Martin
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed May 27 17:21:21 2015 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t4RMLL54030010 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 17:21:21 -0500 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id 7445B53A28B 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 17:21:21 -0500 (CDT) 15, 28 -- X-Virus-Scanned: amavisd-new at znl.com 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id Ox9JbrBOAdNk for {microscopy-at-microscopy.com} ; 15, 28 -- Wed, 27 May 2015 17:21:07 -0500 (CDT) 15, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 3EDD353A277 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2015 17:21:07 -0500 (CDT) 15, 28 -- Message-ID: {55664352.7030204-at-microscopy.com} 15, 28 -- Date: Wed, 27 May 2015 17:21:06 -0500 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:anti-GFP antibody for IEM 15, 28 -- References: {201505272057.t4RKvOse027286-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201505272057.t4RKvOse027286-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201505272057.t4RKvOse027286-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 22, 39 -- From cbuser125-at-gmail.com Mon Jun 1 15:18:38 2015 22, 39 -- Received: from mail-yk0-f181.google.com (mail-yk0-f181.google.com [209.85.160.181]) 22, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t51KIcnV031690 22, 39 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Jun 2015 15:18:38 -0500 22, 39 -- Received: by ykfl8 with SMTP id l8so47138594ykf.1 22, 39 -- for {Microscopy-at-microscopy.com} ; Mon, 01 Jun 2015 13:18:38 -0700 (PDT) 22, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 22, 39 -- d=gmail.com; s=20120113; 22, 39 -- h=from:to:cc:references:in-reply-to:subject:date:message-id 22, 39 -- :mime-version:content-type:content-transfer-encoding:thread-index 22, 39 -- :content-language; 22, 39 -- bh=AcuB0mjibt16LxwW3k1L1764lCC6JEHtzfue+JF8zOI=; 22, 39 -- b=oFQxh1qcH9SiIiq1wIrM/Wb7AjDKjXaDE42xwYnlVq4v3omYYBqIpK5aR5/q+4Litd 22, 39 -- SLA5mEGeaQ4dn8GEsZ1NEeNAM8Uk+g/6V7kk2oDjgio+Jb3DtqEC19bjtBePtdeGqMsz 22, 39 -- 0JckTCozGmUsh6oR+WCwr2BpkI8JKxQ11InGXjJCcY/aBjTQn9+7EAJ0KiQj5nwNHweb 22, 39 -- donLsmQtkuYfCqlaCVU8W8UXK7tgmWEvP5664B1jhPlvHV1rSdvr128uw5j6w43rjyJy 22, 39 -- Budb3BAoy+GT/Kc52DdKclCov3QERCbOs1Y8Aejd+dVDaSn2KSHu4WPSwrG8zKC+Mb/L 22, 39 -- mY1g== 22, 39 -- X-Received: by 10.236.42.201 with SMTP id j49mr25299213yhb.107.1433189918264; 22, 39 -- Mon, 01 Jun 2015 13:18:38 -0700 (PDT) 22, 39 -- Received: from Droseran (71-84-246-54.dhcp.gldl.ca.charter.com. [71.84.246.54]) 22, 39 -- by mx.google.com with ESMTPSA id z10sm12574525yhc.55.2015.06.01.13.18.36 22, 39 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 22, 39 -- Mon, 01 Jun 2015 13:18:37 -0700 (PDT) 22, 39 -- From: "Christopher Buser" {cbuser125-at-gmail.com} 22, 39 -- To: {Microscopy-at-microscopy.com} 22, 39 -- Cc: {schauflingerm-at-missouri.edu} 22, 39 -- References: {201505272256.t4RMu9xk019208-at-ns.microscopy.com} 22, 39 -- In-Reply-To: {201505272256.t4RMu9xk019208-at-ns.microscopy.com} 22, 39 -- Subject: RE: [Microscopy] viaWWW:anti-GFP antibody for IEM 22, 39 -- Date: Mon, 1 Jun 2015 13:18:35 -0700 22, 39 -- Message-ID: {005d01d09ca8$24493280$6cdb9780$-at-gmail.com} 22, 39 -- MIME-Version: 1.0 22, 39 -- Content-Type: text/plain; 22, 39 -- charset="us-ascii" 22, 39 -- Content-Transfer-Encoding: 7bit 22, 39 -- X-Mailer: Microsoft Outlook 15.0 22, 39 -- Thread-Index: AQHSPAhW0H/OxBXkRfKDc7p/K7APPJ2U0xMA 22, 39 -- Content-Language: en-us ==============================End of - Headers==============================
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Message: There are still a few slots open in the upcoming Basic Confocal Microscopy Workshop at the University of South Carolina. Further information is below.
During the week of June 15-19 the University of South Carolina Instrumentation Resource Facility will again host the ÂBasic Confocal Microscopy Workshop. Workshop material is directed towards beginning and intermediate users of confocal microscopes and involves a series of lectures (specimen preparation, labeling strategies, proper set-up of instrument operating parameters, proper handling of 2D and 3D confocal images in programs such as Adobe Photoshop and AMIRA), hands on specimen preparation, and time on a number of different point scanning and spinning disk confocal microscopes.
This is a hands on workshop where participants will stain cells and tissues with multiple labels, collect images, and participate in image analysis exercises using Photoshop and AMIRA.
Faculty will include Dr. Jay Jerome of Vanderbilt University, Dr. Ralph Albrecht of the University of Wisconsin Madison, Dr. John Mackenzie of North Carolina State University, Dr. Tom Trusk of the Medical University of South Carolina, and Dr. Bob Price of the University of South Carolina. Several companies will also have equipment and applications experts on hand to assist with instrumentation and questions about confocal microscopy research protocols.
Cost for the entire week is $350 which covers meals and supplies for the workshop. Please register soon as the workshop has filled the past several years.
For further information please see: http://irf.med.sc.edu/
For questions and registration contact Anna McNeal (anna.mcneal-at-uscmed.sc.edu) or Bob Price (bob.price-at-uscmed.sc.edu)
Bob Price Research Professor Director, Instrumentation Resource Facility School of Medicine, Univ South Carolina Bldg 1 Room B60 6439 GarnerÂs Ferry Road Columbia, SC 29208 803-216-3824 Admin: 803-216-3825
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Email: jegoolsby-at-hotmail.com Name: James Goolsby
Organization: Paragon Bioservices
Title-Subject: [Filtered] Synaptosome Preparation
Message: I am looking for information regarding synaptosome fix/stain/embedding for TEM. Does anyone have experience with this prep and particularly human post-mortem samples?
Has anyone compared preps to determine what works best for visualization of the pre- & post synaptic densities?
Thanks,
James Goolsby, PhD Sr. Scientist Paragon Bioservices Baltimore, MD
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jun 1 17:55:44 2015 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t51Mtipn027718 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 1 Jun 2015 17:55:44 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 2EDE2578FB2 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 1 Jun 2015 17:55:44 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at znl.com 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id TocIFhfZXbEB for {microscopy-at-microscopy.com} ; 13, 28 -- Mon, 1 Jun 2015 17:55:36 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id CD2E3578FAA 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 1 Jun 2015 17:55:36 -0500 (CDT) 13, 28 -- Message-ID: {556CE2E8.3010909-at-microscopy.com} 13, 28 -- Date: Mon, 01 Jun 2015 17:55:36 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Synaptosome Preparation 13, 28 -- References: {201506011722.t51HMVcT021887-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201506011722.t51HMVcT021887-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201506011722.t51HMVcT021887-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: mk-at-seclab.tuwien.ac.at Name: Markus Kammerstetter
Organization: Secure Systems Lab Vienna
Title-Subject: [Filtered] Looking for RJLee PSEM Schematics
Message: Hello,
we have an older model RJ Lee Instruments PSEM Scanning Electron Microscope and we're looking for Service Manuals and Schematics for it. If you have any of those, please let me know.
Thank you, Markus
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jun 1 17:56:25 2015 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t51MuPOx028197 14, 28 -- for {microscopy-at-microscopy.com} ; Mon, 1 Jun 2015 17:56:25 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 1D12B578FCA 14, 28 -- for {microscopy-at-microscopy.com} ; Mon, 1 Jun 2015 17:56:25 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at znl.com 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id Id-glTaWrxjY for {microscopy-at-microscopy.com} ; 14, 28 -- Mon, 1 Jun 2015 17:56:14 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id E0CA4578FC1 14, 28 -- for {microscopy-at-microscopy.com} ; Mon, 1 Jun 2015 17:56:14 -0500 (CDT) 14, 28 -- Message-ID: {556CE30E.7050709-at-microscopy.com} 14, 28 -- Date: Mon, 01 Jun 2015 17:56:14 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Looking for RJLee PSEM Schematics 14, 28 -- References: {201506011723.t51HNW88021938-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201506011723.t51HNW88021938-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201506011723.t51HNW88021938-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From ohn.bullocknewsniyhp-at-gmail.com Mon Jun 1 23:14:35 2015 Return-Path: {ohn.bullocknewsniyhp-at-gmail.com} Received: from gmail.com ([220.241.17.26]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t524EWXC005107 for {microscopylistserverarchive5-at-microscopy.com} ; Mon, 1 Jun 2015 23:14:34 -0500 Message-ID: {48F9725E.AEC1F5EB-at-gmail.com}
Dear TEMers/REMers and OTHers,
I (biologist) have had an animated discussion with a colleague material scientist. The question is: is it possible to measure the size of microparticles (some aluminium-containing mineral) by embedding them, preparing a fine flat surface, coating and analyzing in REM with W gun in BSE mode (I think this is called microprobe analysis in the material science world, far far away in another galaxy). My colleague says it is no problem, really.
I say I see 2 objections (really): - the first one is that the interaction volume is much bigger in BSE mode than in SE mode (Mr Chapman's rule n°3), decreasing the lateral resolution, which is already a problem for particle sizing in REM - the second one is more theoretical: by sectionning microparticles, we get all sorts of cross-sections. By measuring the diameter of the crossections we don't get the measure of the mean diameter of the particles but a much more spread size distribution which is not centered on the actual diameter of the particles.
Des commentaires? Your Comments? Kommentare? Quando se come aqui?
Stephane
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You are right. The problem of size distribution caused by serial sectioning can be overcame by a) Assuming all of the particles are the same size and are randomly distributed in the embedding medium - dangerous assumptions at best or b) Serial sectioning the block and imaging the block face - this requires an ultratome within the SEM (=REM) chamber, but such things exist now: Leighton, S.B. 1981. SEM images of block faces, cut by a miniture microtome within the SEM - a technical note. Scan Electron Microsc. 1981;(Pt 2):73-6. Wanner, A.A., et al. 2015. Challenges of microtome-based serial block-face scanning electron microscopy in neuroscience. J. Microscopy doi: 10.1111/jmi.12244 23 April on-line. or c) Micro-CT within the SEM or by itself.
This still leaves all of the issues in your 1st objection and the basic "where's the edge?" question. Even in secondary imaging this is an issue. How important depends on the particle size - if they're micrometers in size the error won't be too big relative to the particle; if they're nanometers in size, the error can be relatively big. Either way, why not use light or TEM microscopy to size the particles? These would be more accurate.
Phil
} Dear TEMers/REMers and OTHers, } } I (biologist) have had an animated discussion with a colleague material scientist. } The question is: is it possible to measure the size of microparticles (some aluminium-containing mineral) by embedding them, preparing a fine flat surface, coating and analyzing in REM with W gun in BSE mode (I think this is called microprobe analysis in the material science world, far far away in another galaxy). } My colleague says it is no problem, really. } } I say I see 2 objections (really): } - the first one is that the interaction volume is much bigger in BSE mode than in SE mode (Mr Chapman's rule n°3), decreasing the lateral resolution, which is already a problem for particle sizing in REM } - the second one is more theoretical: by sectionning microparticles, we get all sorts of cross-sections. By measuring the diameter of the crossections we don't get the measure of the mean diameter of the particles but a much more spread size distribution which is not centered on the actual diameter of the particles. } } Des commentaires? } Your Comments? } Kommentare? } Quando se come aqui? } } Stephane -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Tue Jun 2 07:35:33 2015 5, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t52CZX9q010697 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jun 2015 07:35:33 -0500 5, 32 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t52CZVR3008902; 5, 32 -- Tue, 2 Jun 2015 08:35:31 -0400 5, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 5, 32 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.195.1; Tue, 2 Jun 2015 08:35:30 -0400 5, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.195.1; Tue, 2 Jun 2015 08:35:30 -0400 5, 32 -- Message-ID: {556DA312.3070900-at-cmich.edu} 5, 32 -- Date: Tue, 2 Jun 2015 08:35:30 -0400 5, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: {nizets2-at-yahoo.com} , micro {microscopy-at-microscopy.com} 5, 32 -- Subject: Re: [Microscopy] Particle sizing with REM 5, 32 -- References: {201506020750.t527o4tE031219-at-ns.microscopy.com} 5, 32 -- In-Reply-To: {201506020750.t527o4tE031219-at-ns.microscopy.com} 5, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-Originating-IP: [141.209.2.100] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 02OzozvXP - d5849a4f32d4 - 20150602 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Dear Stephane, Interaction volume in BSE is bigger than in SE but is not so big if accelerating voltage is low enought. This volume can be small enought, at low voltage, if the mean atomic number of particles is not too poor. Many BSE detectors are now efficient under 5kV; if you can use a FE SEM with a good BSE detector just above the sample (some millimeters) you may obtain a good result. More than lateral resolution the precision of the measure may be affected by the calibration of magnification. For such job it could be interesting to check accuracy of magnification with an appropriate standard. You can easily simulate how far is the interaction volume for your sample using a software like CASINO for example. If you are lucky, may be you will find a good value for accelerationg voltage; not too deep on the sample to avoid resolution problem but enought deep to get BSE from all the volume of the particles. In that case, probably you will see the diameter of the particle and not only the diameter of the sectionned disk on the surface. Of course it supposes good correlation beetween several factors : Z number of particles, Z number of matrix, size of particles, etc...
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique ŕ balayage et microanalyse 2 rue de la Houssiničre BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
Le 02/06/2015 14:54, oshel1pe-at-cmich.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Stephane, } } You are right. } The problem of size distribution caused by serial sectioning can be } overcame by } a) Assuming all of the particles are the same size and are randomly } distributed in the embedding medium - dangerous assumptions at best } or } b) Serial sectioning the block and imaging the block face - this } requires an ultratome within the SEM (=REM) chamber, but such things } exist now: } Leighton, S.B. 1981. SEM images of block faces, cut by a miniture } microtome within the SEM - a technical note. Scan Electron Microsc. } 1981;(Pt 2):73-6. } Wanner, A.A., et al. 2015. Challenges of microtome-based serial } block-face scanning electron microscopy in neuroscience. J. Microscopy } doi: 10.1111/jmi.12244 23 April on-line. } or } c) Micro-CT within the SEM or by itself. } } This still leaves all of the issues in your 1st objection and the basic } "where's the edge?" question. Even in secondary imaging this is an } issue. How important depends on the particle size - if they're } micrometers in size the error won't be too big relative to the particle; } if they're nanometers in size, the error can be relatively big. } Either way, why not use light or TEM microscopy to size the particles? } These would be more accurate. } } Phil } } } Dear TEMers/REMers and OTHers, } } } } I (biologist) have had an animated discussion with a colleague material scientist. } } The question is: is it possible to measure the size of microparticles (some aluminium-containing mineral) by embedding them, preparing a fine flat surface, coating and analyzing in REM with W gun in BSE mode (I think this is called microprobe analysis in the material science world, far far away in another galaxy). } } My colleague says it is no problem, really. } } } } I say I see 2 objections (really): } } - the first one is that the interaction volume is much bigger in BSE mode than in SE mode (Mr Chapman's rule n°3), decreasing the lateral resolution, which is already a problem for particle sizing in REM } } - the second one is more theoretical: by sectionning microparticles, we get all sorts of cross-sections. By measuring the diameter of the crossections we don't get the measure of the mean diameter of the particles but a much more spread size distribution which is not centered on the actual diameter of the particles. } } } } Des commentaires? } } Your Comments? } } Kommentare? } } Quando se come aqui? } } } } Stephane
==============================Original Headers============================== 7, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Tue Jun 2 09:19:33 2015 7, 25 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t52EJUdY000326 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jun 2015 09:19:33 -0500 7, 25 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 7, 25 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id t52EJ9me017832; 7, 25 -- Tue, 2 Jun 2015 16:19:09 +0200 7, 25 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 7, 25 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id t52EJ8x7006786 7, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT); 7, 25 -- Tue, 2 Jun 2015 16:19:08 +0200 7, 25 -- Message-ID: {556DBB8B.2060903-at-univ-nantes.fr} 7, 25 -- Date: Tue, 02 Jun 2015 16:19:55 +0200 7, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 7, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 7, 25 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 7, 25 -- MIME-Version: 1.0 7, 25 -- To: oshel1pe-at-cmich.edu, microscopy-at-microscopy.com 7, 25 -- Subject: Re: [Microscopy] Re: Particle sizing with REM 7, 25 -- References: {201506021254.t52Cs84q023452-at-ns.microscopy.com} 7, 25 -- In-Reply-To: {201506021254.t52Cs84q023452-at-ns.microscopy.com} 7, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-Miltered: at b-mail2 with ID 556DBB5D.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 7, 25 -- X-j-chkmail-Enveloppe: 556DBB5D.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Dear Stephane and colleagues, Why do you like a complicated approach to a simple question? Remove the bulk substrate!
The easiest would be to cast a water or ethanol suspension of your particles on a TEM C-film/Cu 200 mesh grid, then go to see a colleague who runs a TEM or STEM microscope! Too far? Too expensive? No friend? Be self-standing...
1.- First obvious solution: stick your TEM grid on top of a 10mm deep 2mm diameter hole drilled in any light conductive material block (Al alloy is fine). Look at it in SE mode as usual, you will avoid all the trouble of electrons spreading in the substrate, BSE backscattering and secondaries of type 2. Of course the W cathode is not as good as a FEG for ultimate resolution, but nevertheless will give a better resolution than BSE.
2.- Assuming you have a semi-conductor BSE detector, you can easily transform your SEM in S(T)EM microscope. With a bit of manual skill you can carefully mount your BSE detector on the sample table (upside-down to receive the primary probe!) , then with a light U-shape jig hold the TEM grid some 10-15mm above the centre of the BSE detector. The BSE signal becomes a STEM one. However the contrast is not well defined as both directly transmitted (bright field) and scattered electrons (dark field+HAADF) are detected together.
3.- to improve the contrast, you may add a beam stop to catch the transmitted beam and let only the scattered electrons to reach the BSE detector (DF+HAADF). Or you may cover the BSE detector, letting only a 2mm hole to get the directly transmitted beam and BF contrast. Lazy? You may even more easily mount the BSE detector off-centre to block the directly transmitted electrons on its edge and detect only electrons scattered in 2 quadrants (DF+HAADF, 50% signal). The efficiency of detection is high, it means you may use lower beam currents than usual and certainly much lower than for BSE. Expect a better resolution in this STEM mode than in SE. Beware! Contamination may be a limiting factor depending on your vacuum quality and require to work fast, even to record pictures on fresh areas adjacent to that used for focusing. Enjoy Philippe
==============================Original Headers============================== 5, 19 -- From philippe.buffat-at-epfl.ch Tue Jun 2 20:00:56 2015 5, 19 -- Received: from smtp0.epfl.ch (smtp0.epfl.ch [128.178.224.218]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5310t0p006140 5, 19 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jun 2015 20:00:55 -0500 5, 19 -- Received: (qmail 7511 invoked by uid 107); 3 Jun 2015 01:00:52 -0000 5, 19 -- X-Virus-Scanned: ClamAV 5, 19 -- Received: from vpn-253-056.epfl.ch (HELO vpn-253-056.epfl.ch) (128.179.253.56) 5, 19 -- by mail.epfl.ch (AngelmatoPhylax SMTP proxy) with ESMTP; Wed, 03 Jun 2015 03:00:52 +0200 5, 19 -- Message-ID: {556E51C2.2050405-at-epfl.ch} 5, 19 -- Date: Wed, 03 Jun 2015 03:00:50 +0200 5, 19 -- From: Philippe Buffat {philippe.buffat-at-epfl.ch} 5, 19 -- Reply-To: philippe.buffat-at-epfl.ch 5, 19 -- Organization: EPFL 5, 19 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.10; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy-at-microscopy.com 5, 19 -- Subject: RE: Particle sizing with SEM 5, 19 -- Content-Type: text/plain; charset=utf-8; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: Hello, Digital Micrograph records EEL spectra as .dm3 files. While there are scripts that can export the data to a text file - I am wondering whether the reverse is possible? I have a two column .txt file of my EELS data - will it be possible to import that data into Digital Micrograph for further processing? Regards, Debangshu Mukherjee
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Dear Debangshu, There is a feature in the GMS "File" menu called "Import Data ...". This is a fully functioned import tool that handles most data types. For importing EELS data, the spectra most be represented in equally spaced energy bins. If it is just a single row of data with the intensity values, you can convert this to EELS and calibrate using the "Spectrum" menu items.
If the data is in an energy, intensity format, there is no simple way to convert it to EELS data. I have included a short DMScript that handles imported data where the first row (or column) contains the energy values and the second row (or column) contains the EELS counts.
I hope this helps, Ray
Disclosure: I work for Gatan but I am not a programmer. I am sure the code below breaks a dozen rules. I learned scripting as a user of Gatan equipment and continue to use it when analyzing data, trying out new ideas or prototyping. This is unsupported script code.
// ******************** image ConvertImportToEELS( image img ) { number sx, sy number specLength, specDim, d0slice2, d1slice2
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, June 03, 2015 6:13 AM To: ray.twesten-at-sbcglobal.net
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Message: Hello, Digital Micrograph records EEL spectra as .dm3 files. While there are scripts that can export the data to a text file - I am wondering whether the reverse is possible? I have a two column .txt file of my EELS data - will it be possible to import that data into Digital Micrograph for further processing? Regards, Debangshu Mukherjee
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==============================Original Headers============================== 10, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Jun 3 07:58:11 2015 10, 28 -- Received: from znl.com ([206.69.208.20]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t53CwBnP011836 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jun 2015 07:58:11 -0500 10, 28 -- Received: from localhost (localhost [127.0.0.1]) 10, 28 -- by znl.com (Postfix) with ESMTP id 2E2F258B5FD 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jun 2015 07:58:07 -0500 (CDT) 10, 28 -- X-Virus-Scanned: amavisd-new at znl.com 10, 28 -- Received: from znl.com ([127.0.0.1]) 10, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id DLv-YEu8vTUK for {microscopy-at-microscopy.com} ; 10, 28 -- Wed, 3 Jun 2015 07:57:58 -0500 (CDT) 10, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 10, 28 -- by znl.com (Postfix) with ESMTPA id 441FC58B5EE 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jun 2015 07:57:58 -0500 (CDT) 10, 28 -- Message-ID: {556EF9D5.3010801-at-microscopy.com} 10, 28 -- Date: Wed, 03 Jun 2015 07:57:57 -0500 10, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 10, 28 -- MIME-Version: 1.0 10, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 28 -- Subject: viaWWW:Convert EELS to .dm3 10, 28 -- References: {201506030144.t531iLew027002-at-ns.microscopy.com} 10, 28 -- In-Reply-To: {201506030144.t531iLew027002-at-ns.microscopy.com} 10, 28 -- X-Forwarded-Message-Id: {201506030144.t531iLew027002-at-ns.microscopy.com} 10, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 36, 36 -- From ray.twesten-at-sbcglobal.net Wed Jun 3 22:19:17 2015 36, 36 -- Received: from nm4.access.bullet.mail.bf1.yahoo.com (nm4.access.bullet.mail.bf1.yahoo.com [216.109.114.56]) 36, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t543JEJd015257 36, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Jun 2015 22:19:17 -0500 36, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1433387953; bh=gHHIcHRlW9yX5Jsnw8sNO7cUZhcxZ53b+G37UPevSw0=; h=From:To:Cc:References:In-Reply-To:Subject:Date:From:Subject; b=RzuiCZlUYf/CV3zKJHJr8ov0uUV1ZLmMJt7c4N9RKK518XeMuiuibg1oriv3oRho2+tX5mnY0tLDiHQojjzNl1lJDvyuqUB6I+8WBUcB7Bl3Q6Ex8Q/5i9Jf0ows0JT38ekgvHc4nX3I1Iq2SuGjaCEGOoW4Ehh22Z6qKUgKx9XQJmJsrNkJ46TnbrhUdOAINA1Hqr6sWXK8rMwOHdDQjyFWNGavVQrzKbRKAOgzOI2F07Gbru89NaCaIaaSHOZAzG+VNxvpY+ezCC43Pq/9Wqs7W1UlUJNB62yAZVukCMXsMKEwug3fwBguugUE27w5OdZNxTjWAXqlcixhJIAG2g== 36, 36 -- Received: from [66.196.81.157] by nm4.access.bullet.mail.bf1.yahoo.com with NNFMP; 04 Jun 2015 03:19:13 -0000 36, 36 -- Received: from [98.138.104.97] by tm3.access.bullet.mail.bf1.yahoo.com with NNFMP; 04 Jun 2015 03:19:13 -0000 36, 36 -- Received: from [127.0.0.1] by smtp117.sbc.mail.ne1.yahoo.com with NNFMP; 04 Jun 2015 03:19:13 -0000 36, 36 -- X-Yahoo-Newman-Id: 818621.52265.bm-at-smtp117.sbc.mail.ne1.yahoo.com 36, 36 -- X-Yahoo-Newman-Property: ymail-3 36, 36 -- X-YMail-OSG: vSFLyt4VM1m2iEz4LzSRsEGtRBmATDy7vpyXkOYyeJ9.HRk 36, 36 -- eimtckgNWlSxwa.T9KQffWNThV6CrMZ7V.RYs_Is8ItSEF1kj3JdN4XHP2Vh 36, 36 -- YcfDVOlkDuIVPGcWAW1_1GB3zi4zU3szEKmITc7igkS4TiSgPaO5Y3MRCs3C 36, 36 -- VBF4aIafMTJDcq6VStgom6EssYHtFuVVTmL4qsI6LN.4JCPmSGzw4iliwV5M 36, 36 -- zQgxH1pYoHbXYmtot.wGG25SXsgi5ozKq6sI89Y52nyZT5ScQ__zKMnIAePC 36, 36 -- 1jv7gZmpeRyCBb1tjXMGQ4HjZSbD8JERapjwV.CtjvAZOoBp9h9Fr013YJxb 36, 36 -- Yj1pHsnmSDZuzSNXNbPH5VToZ5Xdu65Kr4lfaN.eH1CHjBqZP4UkDEZ0Wng1 36, 36 -- uBeESGMMUvaikjk0.rxQsxuKtA.mSM2sLSqd6sy7bsyZNAlW8sK6A9Sd3GF7 36, 36 -- TanzRl7k4IQz15V3i.dYOem_AeMe_7QaIyxsYYSlqwnAFSPd69gX4NnmQ5Cb 36, 36 -- Yn_sR4.eC3GpGKSaFuL0bxbptH9GCZ88IBH82VRQ2RkPQ_SSHki5JzY5I 36, 36 -- X-Yahoo-SMTP: FjtCMrCswBA518Wrbym5neVt69dnMekrpc0eLz_ppQkuKcL_gU8- 36, 36 -- From: "Ray Twesten" {ray.twesten-at-sbcglobal.net} 36, 36 -- To: {Microscopy-at-microscopy.com} 36, 36 -- Cc: {debangshu-at-psu.edu} 36, 36 -- References: {201506031312.t53DCdm2021271-at-ns.microscopy.com} 36, 36 -- In-Reply-To: {201506031312.t53DCdm2021271-at-ns.microscopy.com} 36, 36 -- Subject: RE: [Microscopy] viaWWW:Convert EELS to .dm3 36, 36 -- Date: Wed, 3 Jun 2015 20:19:13 -0700 36, 36 -- Message-ID: {001501d09e75$3bbb7e90$b3327bb0$-at-sbcglobal.net} 36, 36 -- MIME-Version: 1.0 36, 36 -- Content-Type: text/plain; 36, 36 -- charset="us-ascii" 36, 36 -- Content-Transfer-Encoding: 7bit 36, 36 -- X-Mailer: Microsoft Outlook 14.0 36, 36 -- Thread-Index: AQG8F5sgSe4dGaKuWftkMXsx9amy5p3EtiAA 36, 36 -- Content-Language: en-us ==============================End of - Headers==============================
Does anyone have a dongle for the Kevex AIA2 analysis software for EDX? Thermo was unable to help me.
-Jerry
==============================Original Headers============================== 2, 35 -- From jerry.biehler-at-gmail.com Wed Jun 3 22:51:24 2015 2, 35 -- Received: from mail-qg0-f42.google.com (mail-qg0-f42.google.com [209.85.192.42]) 2, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t543pOlV004295 2, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Jun 2015 22:51:24 -0500 2, 35 -- Received: by qgfa63 with SMTP id a63so12727640qgf.0 2, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 03 Jun 2015 20:51:24 -0700 (PDT) 2, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 35 -- d=gmail.com; s=20120113; 2, 35 -- h=from:content-type:content-transfer-encoding:mime-version:subject 2, 35 -- :message-id:date:to; 2, 35 -- bh=ScgFhWUUma1ShDHJ8dxdEHBYYSunAGmnKf7lSeHW7rg=; 2, 35 -- b=OyfYNL9FGzc5IEbaM4wmXxXQ5W9pVG0m7HQOc8Pj1/pKjp/gPcHypbqNyul8CboNOR 2, 35 -- nTKJ6EbMeyE/m2gFZiyI3H/LvB0uW2Yutx2mTn4fWlyLyFt7Bejx/bxgl5BhgLDfQpaL 2, 35 -- ZFYwm+mEQDdpeZWpSRqtQb9vkIvnFgl7qL2VIFMmelsFANFrFKkqqT/LgPUS/TrD9chI 2, 35 -- lX+JvsedhvZcOZkfPr1z5+WC3OQwcd3irinkUiQXNcFdPCSNSDVtLGdApTVUuWlTtGXM 2, 35 -- 19sRDkg3HqLS41ZfPmKdfGxdqaPT6IIPpSsoWzygRhmq70BytKWJrzcFVZUOq/20pj4k 2, 35 -- QbXw== 2, 35 -- X-Received: by 10.140.92.132 with SMTP id b4mr39371716qge.93.1433389884059; 2, 35 -- Wed, 03 Jun 2015 20:51:24 -0700 (PDT) 2, 35 -- Received: from [10.115.93.35] (mobile-166-176-187-233.mycingular.net. [166.176.187.233]) 2, 35 -- by mx.google.com with ESMTPSA id q74sm1673449qha.4.2015.06.03.20.51.22 2, 35 -- for {Microscopy-at-microscopy.com} 2, 35 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 2, 35 -- Wed, 03 Jun 2015 20:51:22 -0700 (PDT) 2, 35 -- From: Jerry Biehler {jerry.biehler-at-gmail.com} 2, 35 -- Content-Type: text/plain; 2, 35 -- charset=us-ascii 2, 35 -- Mime-Version: 1.0 (1.0) 2, 35 -- Subject: Dongle for Kevex AIA2 2, 35 -- Message-Id: {82CB7ADD-F086-450A-9696-D3017673202A-at-gmail.com} 2, 35 -- Date: Wed, 3 Jun 2015 20:51:19 -0700 2, 35 -- To: Microscopy-at-microscopy.com 2, 35 -- X-Mailer: iPhone Mail (12F70) 2, 35 -- Content-Transfer-Encoding: 8bit 2, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t543pOlV004295 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dsjyeo-at-gmail.com Wed Jun 3 23:35:09 2015 Return-Path: {mike.sfsd4f564s6df45dsjyeo-at-gmail.com} Received: from gmail.com ([1.234.25.150]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t544Z6cL026832 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 3 Jun 2015 23:35:08 -0500 Message-ID: {6655134B.53128F77-at-gmail.com}
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Email: j.werkmann-at-gmail.com Name: werckmann
Organization: INMETRO
Title-Subject: [Filtered] DM4 to DM3
Message: Hello, Every body know how transform DM4 to DM3 format Gatan Thanks for your help Jacques
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jun 4 07:28:04 2015 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t54CS4a5010924 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:28:04 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 8A3CF596CEA 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:28:04 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at znl.com 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id ts3n1Yf6XqaN for {microscopy-at-microscopy.com} ; 11, 28 -- Thu, 4 Jun 2015 07:27:51 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 55B34596CDA 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:27:51 -0500 (CDT) 11, 28 -- Message-ID: {55704446.3040905-at-microscopy.com} 11, 28 -- Date: Thu, 04 Jun 2015 07:27:50 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:DM4 to DM3? 11, 28 -- References: {201506031614.t53GE8YH010476-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201506031614.t53GE8YH010476-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201506031614.t53GE8YH010476-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: UMB cryo-ultramicrotomy mini-course, July 28th and 29th, 2015.
Instructors: Helmut Gnaegi, Diatome Ltd. Maximum number of participants: 6
Dear Colleagues,
We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a cryo-ultramicrotomy mini-course on July 27th and 28th, 2015. The targeted participants of this course are individuals who are experienced with ultramicrotome operation at ambient temperature and wish to extend their skills to include sectioning CEMOVIS and Tokuyasu immmunolabeling specimen. The course will include lectures, demonstration and hands on practice. More information regarding the course and registration can be found in our website
http://www.dental.umaryland.edu/2015ultramicrotomecourse/ Please email coreimaging-at-umaryland.edu for any inquiries.
Thanks.
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jun 4 07:28:59 2015 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t54CSwKw011294 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:28:59 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id 1AA83596D08 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:28:58 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at znl.com 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id HyHJautWMqEB for {microscopy-at-microscopy.com} ; 18, 28 -- Thu, 4 Jun 2015 07:28:46 -0500 (CDT) 18, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id B4742596D00 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:28:46 -0500 (CDT) 18, 28 -- Message-ID: {5570447E.2010209-at-microscopy.com} 18, 28 -- Date: Thu, 04 Jun 2015 07:28:46 -0500 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: viaWWW:UMB cryo-ultramicrotomy mini-course, July 28th and 29th, 2015. 18, 28 -- References: {201506040343.t543hMqh031420-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201506040343.t543hMqh031420-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201506040343.t543hMqh031420-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We're excited to announce the second Oregon Challenges Workshop on Determining the Composition and Structure of Small Volumes on July 30-31, 2015 at the University of Oregon.
The workshop includes two days of talks from experts in electron microscopy, X-ray microscopy, atom probe tomography, and field ion microscopy and will focus on device applications of these techniques.
The full program is available here: https://blogs.uoregon.edu/smallvol2/program
Registration is $160, or $125 for students; the registration form is here: https://blogs.uoregon.edu/smallvol2/registration
If you'll be coming to Portland for M&M, consider coming a few days early and heading down to Eugene for this great workshop. It's only two hours away by train or car. Hope to see you there!
Dr. Benjamin McMorran Assistant Professor of Physics Materials Science Institute and Oregon Center for Optics Department of Physics 1274 University of Oregon Eugene, OR 97403-1274 office phone: 541-346-8624
==============================Original Headers============================== 7, 29 -- From trh-at-uoregon.edu Thu Jun 4 09:30:36 2015 7, 29 -- Received: from smtp.uoregon.edu (oh-smtp4.uoregon.edu [184.171.108.236]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t54EUZt3022132 7, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 09:30:35 -0500 7, 29 -- Received: from webmail.uoregon.edu (webmail2.uoregon.edu [128.223.143.236] (may be forged)) 7, 29 -- (authenticated bits=0) 7, 29 -- by smtp.uoregon.edu (8.14.4/8.14.4) with ESMTP id t54EUVEl003891 7, 29 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-GCM-SHA384 bits=256 verify=NOT) 7, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:30:34 -0700 7, 29 -- Received: from c-73-25-124-121.hsd1.or.comcast.net ([73.25.124.121]) 7, 29 -- by webmail.uoregon.edu 7, 29 -- with HTTP (HTTP/1.1 POST); Thu, 04 Jun 2015 07:30:31 -0700 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain; charset=US-ASCII; 7, 29 -- format=flowed 7, 29 -- Content-Transfer-Encoding: 7bit 7, 29 -- Date: Thu, 04 Jun 2015 07:30:31 -0700 7, 29 -- From: Tyler R Harvey {trh-at-uoregon.edu} 7, 29 -- To: Microscopy-at-microscopy.com 7, 29 -- Subject: Workshop on Determining the Composition and Structure of Small 7, 29 -- Volumes 7, 29 -- Message-ID: {b179a412b0f910c86f292d9558d7be10-at-uoregon.edu} 7, 29 -- X-Sender: trh-at-uoregon.edu 7, 29 -- User-Agent: Roundcube Webmail/1.0.4 7, 29 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.14.151,1.0.33,0.0.0000 7, 29 -- definitions=2015-06-04_08:2015-06-03,2015-06-04,1970-01-01 signatures=0 7, 29 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=5 phishscore=0 7, 29 -- adultscore=0 bulkscore=0 classifier=spam adjust=-40 reason=mlx scancount=1 7, 29 -- engine=7.0.1-1402240000 definitions=main-1506040194 ==============================End of - Headers==============================
Dear Jacques, The DigitalMicrograph application has a "Batch Convert" option that will convert files of various formats. It will allow changing from DM4 to DM3 format. It you do not have the application, a free version can be downloaded from: { http://www.gatan.com/products/tem-analysis/gatan-microscopy-suite-software }
Hope this helps, Ray
Disclaimer: I am an employee of Gatan
Ray D. Twesten Livermore, CA
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, June 04, 2015 5:46 AM To: ray.twesten-at-sbcglobal.net
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Email: j.werkmann-at-gmail.com Name: werckmann
Organization: INMETRO
Title-Subject: [Filtered] DM4 to DM3
Message: Hello, Every body know how transform DM4 to DM3 format Gatan Thanks for your help Jacques
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jun 4 07:28:04 2015 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t54CS4a5010924 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:28:04 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 8A3CF596CEA 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:28:04 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at znl.com 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id ts3n1Yf6XqaN for {microscopy-at-microscopy.com} ; 11, 28 -- Thu, 4 Jun 2015 07:27:51 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 55B34596CDA 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 07:27:51 -0500 (CDT) 11, 28 -- Message-ID: {55704446.3040905-at-microscopy.com} 11, 28 -- Date: Thu, 04 Jun 2015 07:27:50 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:DM4 to DM3? 11, 28 -- References: {201506031614.t53GE8YH010476-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201506031614.t53GE8YH010476-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201506031614.t53GE8YH010476-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 21, 36 -- From ray.twesten-at-sbcglobal.net Thu Jun 4 19:47:56 2015 21, 36 -- Received: from nm25-vm9.access.bullet.mail.gq1.yahoo.com (nm25-vm9.access.bullet.mail.gq1.yahoo.com [216.39.62.72]) 21, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t550lsE3003559 21, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 19:47:56 -0500 21, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1433465274; bh=kRuk1kB4H/m9r5wVp8LokA+1xpdbaxNdikqmJlbZwxU=; h=From:To:Cc:References:In-Reply-To:Subject:Date:From:Subject; b=JQ3QxXNrVfy8JKIaRr3GN7bpWHWu4xWgjOIbCgnPa7TpbaJU9U4MrWYI32jHji3gtU1pTV2QOvwDIWLgjAOgl9LY0BnkpRUlmTpJayn3yW6oIsiTfNTF3Np/bOjfPtAFS7Fu4vJ7wZRaY62MNw66x19sh5xB8MxsFhA2XizpFbCl0V/DZ+WcFTefihKxnW6VE2yw/LstM4haiAPoGPgfrlJ5cdvdphICYRdobrUbfSaJe6WwcUdPqOml0/wE+l2+C9f7jLUG8HFfjuB3RKCAZkiwq9ZN0WyPeSe88wb+Qgg8+ZqHbcAbZwCljSCtOFnZJBuTkF0+WP3xm4C/cGlZmA== 21, 36 -- Received: from [216.39.60.169] by nm25.access.bullet.mail.gq1.yahoo.com with NNFMP; 05 Jun 2015 00:47:54 -0000 21, 36 -- Received: from [98.138.226.244] by tm5.access.bullet.mail.gq1.yahoo.com with NNFMP; 05 Jun 2015 00:47:53 -0000 21, 36 -- Received: from [127.0.0.1] by smtp115.sbc.mail.ne1.yahoo.com with NNFMP; 05 Jun 2015 00:47:53 -0000 21, 36 -- X-Yahoo-Newman-Id: 782174.46529.bm-at-smtp115.sbc.mail.ne1.yahoo.com 21, 36 -- X-Yahoo-Newman-Property: ymail-3 21, 36 -- X-YMail-OSG: 7HH0a2MVM1mhMrNBR6n50lrrSoGzkUPJf_vanNNbrgg8jgF 21, 36 -- hYI5Mf3tCtdOKhLkU6R_3b6Z9dJx6entb3x8toew0YdXaGPveNzGNAVZtvca 21, 36 -- woK6ENyPqVjgTQIMNTgAObGtoe67qP0MENC4cKn5XbTffO_ezLkDeim0MI__ 21, 36 -- wei5gT7kVX9Y2xBm1mrtL0fPylAbafgsMSDwftOUWRXzLAHU8syf_qH_iQ7D 21, 36 -- oCzSyPq_aWEAnShbXBwVC0T40wLqW5kpppbol9kOeu71sS75vyOoJwuW2mDf 21, 36 -- xgSHtDzZiq5utA9ZZJqW3NVWyo8m._YMznmfsCEYFXRl3ZD1eNZXYRMh2WRj 21, 36 -- WRfj2mUIjrgyDlOCGImyo7AqsGcoNRy1BSEb3.IgewZUDxHjO6y4S1mXzoLC 21, 36 -- MSr_SuXM7ONZjXolzFPxgTjRIZLo3nujbDJl3ItxYTcmtIeLVDVNWj9n4d6A 21, 36 -- 0lJua3kAHZDp6.7Iudv7mjB7_KwbyL1p3dRAx3yK3ge1g0xTWS63Ejj2IvfU 21, 36 -- lW7Q7uazO.r1EL0hCN7fMecY6jmArIGi7wXUhFCcZokOmfjAtEj1SFiLO 21, 36 -- X-Yahoo-SMTP: FjtCMrCswBA518Wrbym5neVt69dnMekrpc0eLz_ppQkuKcL_gU8- 21, 36 -- From: "Ray Twesten" {ray.twesten-at-sbcglobal.net} 21, 36 -- To: {Microscopy-at-microscopy.com} 21, 36 -- Cc: {j.werkmann-at-gmail.com} 21, 36 -- References: {201506041246.t54CkQHc028972-at-ns.microscopy.com} 21, 36 -- In-Reply-To: {201506041246.t54CkQHc028972-at-ns.microscopy.com} 21, 36 -- Subject: RE: [Microscopy] viaWWW:DM4 to DM3? 21, 36 -- Date: Thu, 4 Jun 2015 17:47:53 -0700 21, 36 -- Message-ID: {004b01d09f29$42167d30$c6437790$-at-sbcglobal.net} 21, 36 -- MIME-Version: 1.0 21, 36 -- Content-Type: text/plain; 21, 36 -- charset="us-ascii" 21, 36 -- Content-Transfer-Encoding: 7bit 21, 36 -- X-Mailer: Microsoft Outlook 14.0 21, 36 -- Thread-Index: AQIUOB+k9lUYepZxHcZDwtMAdq5I/50V4AVA 21, 36 -- Content-Language: en-us ==============================End of - Headers==============================
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Title-Subject: [Filtered] TEM imaging for food samples
Message: Hi,
I am a PhD candidate at Food Science department in University of Kentucky. My research involves high pressure processing and natural antimicrobials to inhibit pathogens in infant formula and infant cereal.
WeÂd like to use TEM to observe the structural changes in pathogens after high pressure and natural microbial applications.
Since we have food samples, we can not do chemical substitution for TEM observation. We need to prepare the samples with freeze-substitution or high pressure freezing and then do thin sectioning or do CryoTEM. Unfortunately we donÂt have a core facility at our university with freeze substitution or CryoTEM capability.
Is there a freeze-substitution/ high pressure freezing/CryoTEM facility which could process our food samples to visualize bacterial structure?
Thanks
Hayriye
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==============================Original Headers============================== 22, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jun 4 20:48:59 2015 22, 28 -- Received: from znl.com ([206.69.208.20]) 22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t551mx2N025579 22, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 20:48:59 -0500 22, 28 -- Received: from localhost (localhost [127.0.0.1]) 22, 28 -- by znl.com (Postfix) with ESMTP id 3E3D359D3CC 22, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 20:48:59 -0500 (CDT) 22, 28 -- X-Virus-Scanned: amavisd-new at znl.com 22, 28 -- Received: from znl.com ([127.0.0.1]) 22, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 22, 28 -- with ESMTP id j0BaA6sKMtJG for {microscopy-at-microscopy.com} ; 22, 28 -- Thu, 4 Jun 2015 20:48:44 -0500 (CDT) 22, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 22, 28 -- by znl.com (Postfix) with ESMTPA id EE5AF59D3BF 22, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2015 20:48:43 -0500 (CDT) 22, 28 -- Message-ID: {5570FFF5.7030101-at-microscopy.com} 22, 28 -- Date: Thu, 04 Jun 2015 20:48:37 -0500 22, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 22, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 22, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 22, 28 -- MIME-Version: 1.0 22, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 28 -- Subject: viaWWW:TEM imaging for food samples 22, 28 -- References: {201506041434.t54EYqkc025504-at-ns.microscopy.com} 22, 28 -- In-Reply-To: {201506041434.t54EYqkc025504-at-ns.microscopy.com} 22, 28 -- X-Forwarded-Message-Id: {201506041434.t54EYqkc025504-at-ns.microscopy.com} 22, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 22, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Available sometime around October 2015 when the new microscope is delivered. SEM has been under constant service contract. No significant operational issues on any of the components. As I believe this scope has many useful years left in it, I'd hate to see it scavenged for parts or dumpstered.
Feel free to contact me off list with any questions.
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
A programmer walks to the butcher shop and buys a kilo of meat. An hour later he comes back upset that the butcher shortchanged him by 24 grams.
==============================Original Headers============================== 12, 20 -- From jehrman-at-mta.ca Fri Jun 5 06:44:33 2015 12, 20 -- Received: from smtpy.mta.ca (smtpy.mta.ca [138.73.1.168]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t55BiXH1006619 12, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2015 06:44:33 -0500 12, 20 -- Received: from host-22-169.mta.ca ([138.73.22.169]:54474) 12, 20 -- by smtpy.mta.ca with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 12, 20 -- (Exim 4.84) 12, 20 -- (envelope-from {jehrman-at-mta.ca} ) 12, 20 -- id 1Z0q33-00069l-9X 12, 20 -- for Microscopy-at-microscopy.com; Fri, 05 Jun 2015 08:44:29 -0300 12, 20 -- Message-ID: {55718BA2.7090208-at-mta.ca} 12, 20 -- Date: Fri, 05 Jun 2015 08:44:34 -0300 12, 20 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 12, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 12, 20 -- MIME-Version: 1.0 12, 20 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 12, 20 -- Subject: JEOL JSM-5600/Oxford Inca EDS/Deben stage automation available for 12, 20 -- surplus 12, 20 -- Content-Type: text/plain; charset=utf-8; format=flowed 12, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dshgtwy-at-gmail.com Fri Jun 5 17:58:20 2015 Return-Path: {mike.sfsd4f564s6df45dshgtwy-at-gmail.com} Received: from gmail.com ([182.252.177.173]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t55MwEEt019809 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 5 Jun 2015 17:58:16 -0500 Message-ID: {FBA3E3D1.DB0B5769-at-gmail.com}
X-from: vicenzie-at-si.edu
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Email: vicenzie-at-si.edu Name: Edward Vicenzi
Organization: Smithsonian Institution
Title-Subject: [Filtered] Staff position available at the Smithsonian Institution
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==============================Original Headers============================== 14, 38 -- From microscopy.listserver-at-gmail.com Sat Jun 6 16:49:19 2015 14, 38 -- Received: from mail-qk0-f175.google.com (mail-qk0-f175.google.com [209.85.220.175]) 14, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t56LnJjs007567 14, 38 -- for {microscopy-at-microscopy.com} ; Sat, 6 Jun 2015 16:49:19 -0500 14, 38 -- Received: by qkhq76 with SMTP id q76so58653321qkh.2 14, 38 -- for {microscopy-at-microscopy.com} ; Sat, 06 Jun 2015 14:49:19 -0700 (PDT) 14, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 38 -- d=gmail.com; s=20120113; 14, 38 -- h=from:message-id:date:reply-to:user-agent:mime-version:to:subject 14, 38 -- :references:in-reply-to:content-type:content-transfer-encoding; 14, 38 -- bh=t6CZBR12FQktGKDArMrnZsy3/Q1yoBBlmnxT6pYKCuE=; 14, 38 -- b=yiANEjlmy8izs/OH/RJxDsZovmbSudu5fO2OkKl4KKZPTw6T7d6jL9jS8WDukeZbYi 14, 38 -- 4ekjQuRMd2PWKgUzZA1GFe072qwJsI+EeF0fXXYbo0kr40P72rqM9OkCR3orPHfdf/6Z 14, 38 -- BPkVU7mA3q+18obePifTaMs6YTghwjm5NLDq8gzgHusHNNRT1qnqOCYWhTulGQuiQ5K8 14, 38 -- yMGKtoFHQKFHAq0srRAnMlCeHNNrBZkN7IcFQJWevXbDelhjsLHKoH9tW7RBFyVPN3XE 14, 38 -- kMWk+8I+m+oWtWryObyKj8KwFx1oolpx3zvFpGXMvhZVjIXRC+xsqViXMaqGjp7cbXKq 14, 38 -- /9XQ== 14, 38 -- X-Received: by 10.140.233.22 with SMTP id e22mr11581084qhc.67.1433627359286; 14, 38 -- Sat, 06 Jun 2015 14:49:19 -0700 (PDT) 14, 38 -- Received: from Nestors-MacBookAir-Pro-2014.local ([187.84.140.156]) 14, 38 -- by mx.google.com with ESMTPSA id 102sm5862947qgn.37.2015.06.06.14.49.16 14, 38 -- for {microscopy-at-microscopy.com} 14, 38 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 38 -- Sat, 06 Jun 2015 14:49:18 -0700 (PDT) 14, 38 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 38 -- X-Google-Original-From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 38 -- Message-ID: {55736ADA.6040509-at-microscopy.com} 14, 38 -- Date: Sat, 06 Jun 2015 18:49:14 -0300 14, 38 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 38 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 14, 38 -- MIME-Version: 1.0 14, 38 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 38 -- Subject: viaWWW: 14, 38 -- References: {201506051509.t55F9BgC008719-at-ns.microscopy.com} 14, 38 -- In-Reply-To: {201506051509.t55F9BgC008719-at-ns.microscopy.com} 14, 38 -- X-Forwarded-Message-Id: {201506051509.t55F9BgC008719-at-ns.microscopy.com} 14, 38 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 38 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From ferguson651651615poeuu-at-gmail.com Sat Jun 6 20:57:17 2015 Return-Path: {ferguson651651615poeuu-at-gmail.com} Received: from gmail.com ([218.147.241.154]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t571vBAf032039 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 6 Jun 2015 20:57:13 -0500 Message-ID: {9E6D2D31.34A154A9-at-gmail.com}
X-from: romina.cialdella-at-nih.gov
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Title-Subject: [Filtered] Register for Frontiers in Light Microscopy, Nov. 17, 2015
Message: Register for Frontiers in Light Microscopy
The Center for Cancer Research at the National Cancer Institute invites you to a one-day national symposium entitled "Frontiers in Light Microscopy" on November 17, 2015 at the National Institutes of Health main campus in Bethesda, Maryland. The program includes recent advances in the field and should be an exciting forum for discussion and debate on the current state of the field.
Speakers:  Peter Friedl  Hari Shroff  Roberto Weigert  Wesley Legant  Samie Jaffrey  Klaus Hahn  Daniel Larson  Joerg Bewersdorf  Jennifer Lippincott-Schwartz  Na Ji
Registration is free, but seating is limited so be sure to register early.
Please mark November 17, 2015 on your calendar for this exciting event, and forward this email to colleagues.
For conference-related questions, please contact conferenceplanning-at-mail.nih.gov.
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We've AGAIN updated our free image processing instructions from the NUANCE Center at Northwestern University. A few additions have been made to include explanations of file types, how to resize images the right way, and even some new coloring instructions. All of which are designed to be super easy and will walk the user step-by-(sometimes painful) step to do all sorts of normal image processing procedures, to several different procedures to apply false color to an image. THE EXTRA SPECIAL PART is the chapter on what I call Multi-Detector Color. Now I know I did not invent this technique, BUT I've worked out some pretty simple procedures that will allow you to make these really fantastic color images, even if you only have 1 SE detector in your SEM. It's totally free, so please check it out and let us know what you think.
ERiC Jay Miller Microscopy & Imaging Specialist Electron Probe Instrumentation Center
Northwestern University Mail: 2036 Cook Hall Office: 1152 Cook Hall 2220 Campus Drive Evanston, IL 60208-3108
ph: (847) 467-0789
http://www.nuance.northwestern.edu
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From benny0439655322345345t-at-gmail.com Wed Jun 10 21:21:33 2015 Return-Path: {benny0439655322345345t-at-gmail.com} Received: from gmail.com ([210.121.164.118]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t5B2LVK0003540 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 10 Jun 2015 21:21:32 -0500 Message-ID: {3F2F2EC8.63F50C97-at-gmail.com}
Hello everyone,
I am looking for a program that will help me calculate beam broadening for STEM-EDS measurements. In particular, Iâd like to figure out the maximum achievable spatial resolution given a known sample composition, zone axis, thickness, energy, and so on. Does anyone know if there is a freely available program that can help me with these calculations?
Thanks! ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
On Jun 11, 2015, at 5:18 PM, steven.spurgeon-at-pnnl.gov wrote:
} Hello everyone, } } I am looking for a program that will help me calculate beam } broadening for STEM-EDS measurements. In particular, Id like to } figure out the maximum achievable spatial resolution given a known } sample composition, zone axis, thickness, energy, and so on. Does } anyone know if there is a freely available program that can help me } with these calculations? } } Thanks! } ______________________________________ } Steven R. Spurgeon, Ph.D. } Postdoctoral Research Associate } Fundamental and Computational Sciences Directorate } } Pacific Northwest National Laboratory } 902 Battelle Boulevard } P.O. Box 999 MSIN:K8-87 } Richland, WA 99352 } } Tel: +1-509-371-7123 } steven.spurgeon-at-pnnl.gov } www.stevenspurgeon.com {http://www.stevenspurgeon.com/} } www.pnnl.gov {http://www.pnnl.gov/} } Dear Steven, David Joy wrote such a program some time ago. I hope someone else on the list knows how to get a hold of it. Yours, Bill
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From advertise.bz222vhhoa-at-gmail.com Thu Jun 11 22:28:07 2015 Return-Path: {advertise.bz222vhhoa-at-gmail.com} Received: from gmail.com ([222.99.124.120]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t5C3S4MV028189 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 11 Jun 2015 22:28:06 -0500 Message-ID: {72BF94BA.1910C21C-at-gmail.com}
The 2015 ImageJ User and Developer Conference (http://conference.imagej.net/) will be in Madison, Wisconsin on September 3rd and 4th, and you're invited to take part!
This conference will complement the European meetings in Luxembourg and will offer workshops to improve software knowledge and usage for both beginner/user and advanced/developer topics. The conference will provide a community forum for ImageJ development over the past three years and will also feature shorter presentations highlighting case studies, plugins, and solutions to common problems. Submissions from the ImageJ community for 10 minute "lightning talk" presentations; 60 to 120 minute workshops; and scientific posters for the parallel poster session are warmly encouraged and those who wish to attend and/or participate should apply by July 1st.
Submissions for all audiences - from novice user to advanced developer - are welcome. The focus of the conference is on community, so tools being presented must be made publicly available before the start of the conference.
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Monday, the start of another workweek but I thought I'd ask a question of the collective wisdom.
How many decimal points do you report or believe when making measurements with:
SEM? TEM? LM?
Do you report numbers suggesting you can measure to one thousandth of a nanometer? How about a hundredth of a micron or micrometer? What about EDS analysis? On a non-flat, non-homogeneous sample can you actually measure to a tenth of 1 wt%? How about hundredth?
Will I get a lot of Out-of-Office responses and do I believe them?
Questions on Monday morning to start the gray matter working.\
Stay safe................ Frank
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==============================Original Headers============================== 13, 28 -- From frank_karl-at-ardl.com Mon Jun 15 07:12:32 2015 13, 28 -- Received: from cal1-mh779.smtproutes.com (cal1-mh779.smtproutes.com [208.70.91.145]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5FCCWW1025934 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 07:12:32 -0500 13, 28 -- X-Katharion-ID: 1434370330.87475.cal1-mh779 13, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by 13, 28 -- cal1-mh779.smtproutes.com [(192.69.16.145)] with ESMTP via TCP 13, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 15 Jun 2015 12:12:10 +0000 13, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 13, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 15 13, 28 -- Jun 2015 08:12:10 -0400 13, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 13, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 13, 28 -- {microscopy-at-microscopy.com} 13, 28 -- Date: Mon, 15 Jun 2015 08:12:09 -0400 13, 28 -- Subject: meassurements: It's how small? 13, 28 -- Thread-Topic: meassurements: It's how small? 13, 28 -- Thread-Index: AdCnZID1Qkvf8eMpRwutUMpq1aZTdQ== 13, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772CBF0B0518-at-exchange2k7.ad.ardl.com} 13, 28 -- Accept-Language: en-US 13, 28 -- Content-Language: en-US 13, 28 -- X-MS-Has-Attach: 13, 28 -- X-MS-TNEF-Correlator: 13, 28 -- acceptlanguage: en-US 13, 28 -- Content-Type: text/plain; charset="us-ascii" 13, 28 -- MIME-Version: 1.0 13, 28 -- Content-Transfer-Encoding: 8bit 13, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5FCCWW1025934 ==============================End of - Headers==============================
You can also try using the Monte Carlo programs by Gauvin and Demers group (WinXray and Casino) or DTSA-II (N. Ritchie, NIST). The cross-sections are more accurate for SEM work, but they should be good enough to give you an idea of your beam spreading. DTSA-II has an option for a thin film on substrate and you can define the substrate as "none".
Win X-ray MC X-ray http://montecarlomodeling.mcgill.ca/software/softwareprojects.html
On 6/11/2015 8:05 PM, steven.spurgeon-at-pnnl.gov wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello everyone, } } I am looking for a program that will help me calculate beam broadening for STEM-EDS measurements. In particular, Iâd like to figure out the maximum achievable spatial resolution given a known sample composition, zone axis, thickness, energy, and so on. Does anyone know if there is a freely available program that can help me with these calculations? } } Thanks! } ______________________________________ } Steven R. Spurgeon, Ph.D. } Postdoctoral Research Associate } Fundamental and Computational Sciences Directorate } } Pacific Northwest National Laboratory } 902 Battelle Boulevard } P.O. Box 999 MSIN:K8-87 } Richland, WA 99352 } } Tel: +1-509-371-7123 } steven.spurgeon-at-pnnl.gov } www.stevenspurgeon.com {http://www.stevenspurgeon.com/} } www.pnnl.gov {http://www.pnnl.gov/} } } } ==============================Original Headers============================== } 6, 26 -- Fromprvs=598c7a13d=steven.spurgeon-at-pnnl.gov Thu Jun 11 19:02:48 2015 } 6, 26 -- Received: from Emailgw01.pnnl.gov (emailgw01.pnnl.gov [192.101.109.61]) } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5C02m4M013579 } 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Jun 2015 19:02:48 -0500 } 6, 26 -- Received: from ex10cashub05.pnnl.gov ([130.20.128.106]) } 6, 26 -- by Emailgw01.pnnl.gov with ESMTP/TLS/AES128-SHA; 11 Jun 2015 17:02:47 -0700 } 6, 26 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.189]) by EX10CASHUB05.pnnl.gov } 6, 26 -- ([130.20.128.106]) with mapi id 14.03.0224.002; Thu, 11 Jun 2015 17:02:26 } 6, 26 -- -0700 } 6, 26 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} } 6, 26 -- To:"Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 6, 26 -- Subject: Beam broadening calculations for STEM-EDS } 6, 26 -- Thread-Topic: Beam broadening calculations for STEM-EDS } 6, 26 -- Thread-Index: AQHQpKMQ0e1BzAHgskKlJb0XA0womQ== } 6, 26 -- Date: Fri, 12 Jun 2015 00:02:25 +0000 } 6, 26 -- Message-ID: {6A8E207A-C2C9-49AA-B219-4115EDD1A7F2-at-pnnl.gov} } 6, 26 -- Accept-Language: en-US } 6, 26 -- Content-Language: en-US } 6, 26 -- X-MS-Has-Attach: } 6, 26 -- X-MS-TNEF-Correlator: } 6, 26 -- x-originating-ip: [130.20.128.10] } 6, 26 -- Content-Type: text/plain; charset="utf-8" } 6, 26 -- Content-ID: {23B9B2DC6F93CE45BFAA24D18F1EE5D8-at-pnnl.gov} } 6, 26 -- MIME-Version: 1.0 } 6, 26 -- Content-Transfer-Encoding: 8bit } 6, 26 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t5C02m4M013579 } ==============================End of - Headers==============================
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
I would contend, that you are asking the wrong question. The subject should be accuracy and precision of a measurement.
It is not how many digits you report after a measurement, but more importantly, reporting of the standard error with any measurement be it dimension, composition or whatever. Both accuracy and precision.
Without an measurement error estimate then the number of digits is irrelevant.
My 2 cents.
Nestor Your Friendly Neighborhood SysOp
BTW, I chuckle at seeing measurements reported more than 3 significant figures and no error bars.
On Jun 15, 2015, at 7:12 AM CDT, {frank_karl-at-ardl.com} {frank_karl-at-ardl.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Monday, the start of another workweek but I thought I'd ask a question of the collective wisdom. } } How many decimal points do you report or believe when making measurements with: } } SEM? } TEM? } LM? } } Do you report numbers suggesting you can measure to one thousandth of a nanometer? How about a hundredth of a micron or micrometer? What about EDS analysis? On a non-flat, non-homogeneous sample can you actually measure to a tenth of 1 wt%? How about hundredth? } } } Will I get a lot of Out-of-Office responses and do I believe them? } } Questions on Monday morning to start the gray matter working.\ } } Stay safe................ } Frank } } } } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } } ==============================Original Headers============================== } 13, 28 -- From frank_karl-at-ardl.com Mon Jun 15 07:12:32 2015 } 13, 28 -- Received: from cal1-mh779.smtproutes.com (cal1-mh779.smtproutes.com [208.70.91.145]) } 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5FCCWW1025934 } 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 07:12:32 -0500 } 13, 28 -- X-Katharion-ID: 1434370330.87475.cal1-mh779 } 13, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by } 13, 28 -- cal1-mh779.smtproutes.com [(192.69.16.145)] with ESMTP via TCP } 13, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 15 Jun 2015 12:12:10 +0000 } 13, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by } 13, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 15 } 13, 28 -- Jun 2015 08:12:10 -0400 } 13, 28 -- From: Frank Karl {frank_karl-at-ardl.com} } 13, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" } 13, 28 -- {microscopy-at-microscopy.com} } 13, 28 -- Date: Mon, 15 Jun 2015 08:12:09 -0400 } 13, 28 -- Subject: meassurements: It's how small? } 13, 28 -- Thread-Topic: meassurements: It's how small? } 13, 28 -- Thread-Index: AdCnZID1Qkvf8eMpRwutUMpq1aZTdQ== } 13, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772CBF0B0518-at-exchange2k7.ad.ardl.com} } 13, 28 -- Accept-Language: en-US } 13, 28 -- Content-Language: en-US } 13, 28 -- X-MS-Has-Attach: } 13, 28 -- X-MS-TNEF-Correlator: } 13, 28 -- acceptlanguage: en-US } 13, 28 -- Content-Type: text/plain; charset="us-ascii" } 13, 28 -- MIME-Version: 1.0 } 13, 28 -- Content-Transfer-Encoding: 8bit } 13, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5FCCWW1025934 } ==============================End of - Headers==============================
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center NanoScience and Technology Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
I am one of the author of some of the program mentioned and I want to add a warning. All MC programs mentioned does not take into account the crystal structure of the sample, they consider the sample amorphous. So you will get the beam broadening without channeling effect (or orientation effect). For more information, we published a paper on the lateral resolution in STEM mode with CASINO v3 in amorphous sample: Demers, H.; Ramachandra, R.; Drouin, D. & de Jonge, N. The Probe Profile and Lateral Resolution of Scanning Transmission Electron Microscopy of Thick Specimens *Microscopy and Microanalysis, **2012**, 18*, 582-590 DOI: 10.1017/S1431927612000232
Regards, Hendrix
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Good point! ALL the MC programs I mentioned assume an amorphous sample. I had missed the fact that Steve had included channeling in his requirements.
I believe that Dave Muller's group at Cornell has taken channeling into account in their atomic resolution STEM calculations. For non-atomic resolution work, channeling may safely be neglected in most cases. It is also likely that beam convergence angle will have a more significant effect on resolution than channeling. For example, in organic samples {100nm, Muller's group indicate that the beam convergence has a greater effect than the beam spreading (Ultra. v.109 p.1 2008).
Cheers, Henk
On 6/15/2015 10:13 AM, drix00-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Steve and Henk, } } I am one of the author of some of the program mentioned and I want to add a } warning. All MC programs mentioned does not take into account the crystal } structure of the sample, they consider the sample amorphous. So you will } get the beam broadening without channeling effect (or orientation effect). } For more information, we published a paper on the lateral resolution in } STEM mode with CASINO v3 in amorphous sample: } Demers, H.; Ramachandra, R.; Drouin, D. & de Jonge, N. } The Probe Profile and Lateral Resolution of Scanning Transmission Electron } Microscopy of Thick Specimens } *Microscopy and Microanalysis, **2012**, 18*, 582-590 } DOI: 10.1017/S1431927612000232 } } } Regards, } Hendrix } } ==============================Original Headers============================== } 4, 26 -- From drix00-at-gmail.com Mon Jun 15 09:12:16 2015 } 4, 26 -- Received: from mail-yh0-f52.google.com (mail-yh0-f52.google.com [209.85.213.52]) } 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5FECG6C024053 } 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 09:12:16 -0500 } 4, 26 -- Received: by yhpn97 with SMTP id n97so43352053yhp.0 } 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 07:12:14 -0700 (PDT) } 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 26 -- d=gmail.com; s=20120113; } 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; } 4, 26 -- bh=DFaVS+UPzHW5nnkUErw770XYgjzk38ouFKjAODdx8sM=; } 4, 26 -- b=Vcg3E3/2wjb0EY738rxIGuUlKmPbwnsD7eUbBfpaKSLrrN40aq9fLorL+Ch2mTcbg/ } 4, 26 -- wCvYGfAhTICHEd1wvR8+srleAGB/VJ5oiiLDJshHXOyTzUAcdL2aUyQROobW1yGbEYmV } 4, 26 -- m3FYCFLw9UOY0mrcPyeds0YacTEc9fkdGZrkuK9gfXBful+qF6F6eQU7KOV1i6s7cy3R } 4, 26 -- EeZrdaPJuvGmEGADXqH29qJlntJMQNz0qrnpR/5+SFg0bA/dldFVNJir/wB4lQ6InFeW } 4, 26 -- jR+FipQw/pnFA06TkzAgss5JzSapKaSLzRWai3ZGxq9H9HeTI3AxDZ5zVbHGSUsP6VEd } 4, 26 -- Q/Yw== } 4, 26 -- MIME-Version: 1.0 } 4, 26 -- X-Received: by 10.13.231.132 with SMTP id q126mr34097152ywe.14.1434377534429; } 4, 26 -- Mon, 15 Jun 2015 07:12:14 -0700 (PDT) } 4, 26 -- Received: by 10.37.207.196 with HTTP; Mon, 15 Jun 2015 07:12:14 -0700 (PDT) } 4, 26 -- Date: Mon, 15 Jun 2015 10:12:14 -0400 } 4, 26 -- Message-ID: {CAO806QQXwNgwrGYazoYmtXaRjnfiRkpaZNs13n=t47o6AJLQhw-at-mail.gmail.com} } 4, 26 -- Subject: Re: Beam broadening calculations for STEM-EDS } 4, 26 -- From: drix {drix00-at-gmail.com} } 4, 26 -- To: Microscopy-at-microscopy.com } 4, 26 -- Content-Type: text/plain; charset=UTF-8 } ==============================End of - Headers==============================
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
Iâve been trying to decipher the quantification options for our STEM-EDS analysis program. The program is called âAnalysis Stationâ and its sub-program is âMappingProgram.â
It gives me three options:
* ZAF * Ratio * NET Int.
I have figured out what ZAF is, but Iâm not sure about the other two. Does anyone know if these routines automatically deconvolute overlapping peaks (judging by the appearance of my maps they doâŚ)? Moreover, does anyone happen to have a manual that describes what the various options are for each routine?
Thanks! ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
The JEOL EDS software works on both SEMs and TEMs and the three different quantification methods are tailored to those two different microscopy techniques. In short, the quantification routines are used to compute the k-factors for quantitative analysis. The ZAF routine is a method to compute matrix corrections for the effects of atomic number, likelihood of absorption, and characteristic fluorescence of emitted x-rays from your sample, and these corrections are generally only valid when you have the classic teardrop interaction volume (i.e. bulk samples). So this would be a good routine to correct maps acquired via SEM.
The ratio technique is the appropriate technique for maps acquired via TEM as TEM samples are typically much too thin for the emitted x-rays to be influenced in any statistically significant manner by Z, A, or F (you might still need to worry about channeling effects induced by sample orientation, however). This routine uses precomputed k-factors or experimentally calculated k-factors as anchor points from which the quantitative composition of your sample is derived, as the ratio of peak intensities from a known material and an unknown material is directly related to the k-factor. Selecting the ratio routine fills in the peak intensity of a known material and the k-factor, allowing the software to calculate the concentration from the measured peak intensities of your unknown sample. Obviously your results will be much more quantitative should you experimentally calculate k-factors for the elements of interest (and under identical illumination conditions) than by using the software database.
X-from the JEOL EDS software manual regarding the net intensity routine:
"The net intensity map displays relative intensities after deconvolution and back-ground subtraction. The result of the net intensity map is different between with check in the â100%â and without. Check â100%â Concentration before correcting by Quant. Method displays. The summation of K-ratios for all elements is unified at each pixel. Uncheck â100%â Normalize the maximum net intensity to 255 in the all pixel of the element."
Practically speaking, you'll want to be exceedingly careful generating quantitative EDS data without first experimentally generating appropriate k-factors. Without doing so, your quantitative numbers are going to be ballpark estimates for anything but the simplest samples. Such data might not be trustworthy by itself but it can be useful as a comparison between EDS data from different unknowns acquired under similar conditions. Masashi Watanabe and David Williams discuss the problems associated with acquisition of quantitative EDS data and an alternative to using k-factors in a series of papers (see M. Watanabe's website at Lehigh) and in the second edition of the classic Williams and Carter intro to TEM text.
Good luck, Chris
On Mon, Jun 15, 2015 at 3:47 PM, {steven.spurgeon-at-pnnl.gov} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello everyone, } } Iâve been trying to decipher the quantification options for our STEM-EDS analysis program. The program is called âAnalysis Stationâ and its sub-program is âMappingProgram.â } } It gives me three options: } } * ZAF } * Ratio } * NET Int. } } I have figured out what ZAF is, but Iâm not sure about the other two. Does anyone know if these routines automatically deconvolute overlapping peaks (judging by the appearance of my maps they doâŚ)? Moreover, does anyone happen to have a manual that describes what the various options are for each routine? } } Thanks! } ______________________________________ } Steven R. Spurgeon, Ph.D. } Postdoctoral Research Associate } Fundamental and Computational Sciences Directorate } } Pacific Northwest National Laboratory } 902 Battelle Boulevard } P.O. Box 999 MSIN:K8-87 } Richland, WA 99352 } } Tel: +1-509-371-7123 } steven.spurgeon-at-pnnl.gov } www.stevenspurgeon.com {http://www.stevenspurgeon.com/} } www.pnnl.gov {http://www.pnnl.gov/} } } } ==============================Original Headers============================== } 9, 26 -- From prvs=60155df7a=steven.spurgeon-at-pnnl.gov Mon Jun 15 14:37:45 2015 } 9, 26 -- Received: from Emailgw01.pnnl.gov (emailgw01.pnnl.gov [192.101.109.61]) } 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5FJbiT6007840 } 9, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 14:37:44 -0500 } 9, 26 -- Received: from ex10cashub02.pnnl.gov ([130.20.128.185]) } 9, 26 -- by Emailgw01.pnnl.gov with ESMTP/TLS/AES128-SHA; 15 Jun 2015 12:37:43 -0700 } 9, 26 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.169]) by ex10cashub02.pnnl.gov } 9, 26 -- ([130.20.128.185]) with mapi id 14.03.0224.002; Mon, 15 Jun 2015 12:37:43 } 9, 26 -- -0700 } 9, 26 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} } 9, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 9, 26 -- Subject: JEOL STEM-EDS Software Quantification Options } 9, 26 -- Thread-Topic: JEOL STEM-EDS Software Quantification Options } 9, 26 -- Thread-Index: AQHQp6K/Cd0S5GxkAE+bGYRysqpMJQ== } 9, 26 -- Date: Mon, 15 Jun 2015 19:37:42 +0000 } 9, 26 -- Message-ID: {0CB7FA56-4984-4EE3-86E9-69A448798CA3-at-pnnl.gov} } 9, 26 -- Accept-Language: en-US } 9, 26 -- Content-Language: en-US } 9, 26 -- X-MS-Has-Attach: } 9, 26 -- X-MS-TNEF-Correlator: } 9, 26 -- x-originating-ip: [130.20.128.10] } 9, 26 -- Content-Type: text/plain; charset="utf-8" } 9, 26 -- Content-ID: {2AFC3367B6CF4141B7BC1B1E03DD5058-at-pnnl.gov} } 9, 26 -- MIME-Version: 1.0 } 9, 26 -- Content-Transfer-Encoding: 8bit } 9, 26 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t5FJbiT6007840 } ==============================End of - Headers==============================
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==============================Original Headers============================== 18, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jun 15 18:46:15 2015 18, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5FNkFkK023264 18, 17 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 18:46:15 -0500 18, 17 -- Message-ID: {557F63C7.1000208-at-microscopy.com} 18, 17 -- Date: Mon, 15 Jun 2015 18:46:15 -0500 18, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 18, 17 -- MIME-Version: 1.0 18, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 17 -- Subject: viaWWW:Job Opportunity at Bruker Nano 18, 17 -- References: {201506152008.t5FK8v5J029479-at-ns.microscopy.com} 18, 17 -- In-Reply-To: {201506152008.t5FK8v5J029479-at-ns.microscopy.com} 18, 17 -- X-Forwarded-Message-Id: {201506152008.t5FK8v5J029479-at-ns.microscopy.com} 18, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On a related point, inaccuracy of standardless "quantitative" EDS analyses can lead to substantial errors. An approach by Dale Newbury (Joseph Goldstein, Dale E. Newbury, David C. Joy, Charles E. Lyman, Patrick Echlin, Eric Lifshin, Linda Sawyer, J.R. Michael, Scanning Electron Microscopy and X-ray Microanalysis: Third Ed., Springer, 2012, 355) is that numerical values for concentration of such analyses are reported as a range. Specifically, the concentration of an element is represented by one of three categories: Major: } 10 wt%; Minor: 1-10 wt%; and Trace: { 1 wt%.
The lab I retired from used this approach to give our customers some sense of the prevalence of an element in a qualitative analysis. After acquiring a qualitative EDS spectrum, we ran a quick standardless quantitative analysis and reported the "concentrations" as major, minor or trace, as described above. Furthermore, we did not divulge to our customers the concentration ranges that the three categories represented. This solved several problems, one of which was that we did not provide our internal customer a potentially worthless number that could be used as the final word on concentration of the element in the sample. My apologies to Dale for any misstatements.
I would contend, that you are asking the wrong question. The subject should be accuracy and precision of a measurement.
It is not how many digits you report after a measurement, but more importantly, reporting of the standard error with any measurement be it dimension, composition or whatever. Both accuracy and precision. Without an measurement error estimate then the number of digits is irrelevant.
My 2 cents.
Nestor Your Friendly Neighborhood SysOp
BTW, I chuckle at seeing measurements reported more than 3 significant figures and no error bars.
On Jun 15, 2015, at 7:12 AM CDT, {frank_karl-at-ardl.com} {frank_karl-at-ardl.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Monday, the start of another workweek but I thought I'd ask a question of the collective wisdom. } } How many decimal points do you report or believe when making measurements with: } } SEM? } TEM? } LM? } } Do you report numbers suggesting you can measure to one thousandth of a nanometer? How about a hundredth of a micron or micrometer? What about EDS analysis? On a non-flat, non-homogeneous sample can you actually measure to a tenth of 1 wt%? How about hundredth? } } } Will I get a lot of Out-of-Office responses and do I believe them? } } Questions on Monday morning to start the gray matter working.\ } } Stay safe................ } Frank } } } } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } } ==============================Original Headers============================== } 13, 28 -- From frank_karl-at-ardl.com Mon Jun 15 07:12:32 2015 } 13, 28 -- Received: from cal1-mh779.smtproutes.com (cal1-mh779.smtproutes.com [208.70.91.145]) } 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5FCCWW1025934 } 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 07:12:32 -0500 } 13, 28 -- X-Katharion-ID: 1434370330.87475.cal1-mh779 } 13, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by } 13, 28 -- cal1-mh779.smtproutes.com [(192.69.16.145)] with ESMTP via TCP } 13, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 15 Jun 2015 12:12:10 +0000 } 13, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by } 13, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 15 } 13, 28 -- Jun 2015 08:12:10 -0400 } 13, 28 -- From: Frank Karl {frank_karl-at-ardl.com} } 13, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" } 13, 28 -- {microscopy-at-microscopy.com} } 13, 28 -- Date: Mon, 15 Jun 2015 08:12:09 -0400 } 13, 28 -- Subject: meassurements: It's how small? } 13, 28 -- Thread-Topic: meassurements: It's how small? } 13, 28 -- Thread-Index: AdCnZID1Qkvf8eMpRwutUMpq1aZTdQ== } 13, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772CBF0B0518-at-exchange2k7.ad.ardl.com} } 13, 28 -- Accept-Language: en-US } 13, 28 -- Content-Language: en-US } 13, 28 -- X-MS-Has-Attach: } 13, 28 -- X-MS-TNEF-Correlator: } 13, 28 -- acceptlanguage: en-US } 13, 28 -- Content-Type: text/plain; charset="us-ascii" } 13, 28 -- MIME-Version: 1.0 } 13, 28 -- Content-Transfer-Encoding: 8bit } 13, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5FCCWW1025934 } ==============================End of - Headers==============================
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center NanoScience and Technology Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
==============================Original Headers============================== 22, 38 -- From anl.nestor.zaluzec-at-gmail.com Mon Jun 15 08:09:23 2015 22, 38 -- Received: from mail-ie0-f178.google.com (mail-ie0-f178.google.com [209.85.223.178]) 22, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5FD9MZp022326 22, 38 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 08:09:22 -0500 22, 38 -- Received: by iebmu5 with SMTP id mu5so61845784ieb.1 22, 38 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 06:09:22 -0700 (PDT) 22, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 22, 38 -- d=gmail.com; s=20120113; 22, 38 -- h=sender:content-type:mime-version:subject:from:in-reply-to:date:cc 22, 38 -- :content-transfer-encoding:message-id:references:to; 22, 38 -- bh=iPg42v6KTKE6jTY1aGXtqkJoEBTPNUaBB8AGEeu7wAo=; 22, 38 -- b=Du0htZv5I5L+gPPxzkvE1hcde2t6wDIr26o4bmTBdaJ26xykr0sUwG7Vx2EEDd9cpu 22, 38 -- ClaY5KB2BgC/LqMXFAaKG0CE6t1OK+svasMbmbWXDhecsPgcEvG50YXrzNkmXo+1KFni 22, 38 -- ZQ0gmAkCGz8bQaQbnLq6ketYYz0neDuQ2YnrAooU/6R007ezI5FdNnEqwTvK7sC73Sjn 22, 38 -- iysN0MyZSDJq60E9CeYb8Fq7Se5Vi85QLkqzKZ0hN+oO61xmvHyfRmNj1a4YnlbR+CaX 22, 38 -- yNNQ/koyh7xqOMlw2yo7Z9y+QNuvXIYWv9eEEjImbZMUVH0q01pZQrj5Og5DwS4nnJiy 22, 38 -- Slpw== 22, 38 -- X-Received: by 10.107.31.134 with SMTP id f128mr34329165iof.19.1434373762350; 22, 38 -- Mon, 15 Jun 2015 06:09:22 -0700 (PDT) 22, 38 -- Received: from [10.0.0.6] ([67.176.143.24]) 22, 38 -- by mx.google.com with ESMTPSA id 82sm1556216ioh.3.2015.06.15.06.09.20 22, 38 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 22, 38 -- Mon, 15 Jun 2015 06:09:21 -0700 (PDT) 22, 38 -- Sender: "ANL.Nestor Zaluzec" {anl.nestor.zaluzec-at-gmail.com} 22, 38 -- Content-Type: text/plain; charset=us-ascii 22, 38 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.6\)) 22, 38 -- Subject: Re: [Microscopy] measurements: It's how small? or more importantly what is your accuracy and precision.... 22, 38 -- From: "Nestor J. Zaluzec - ANL/GAccnt" {zaluzec-at-aaem.amc.anl.gov} 22, 38 -- In-Reply-To: {201506151212.t5FCCY5o025941-at-ns.microscopy.com} 22, 38 -- Date: Mon, 15 Jun 2015 08:09:20 -0500 22, 38 -- Cc: "Zaluzec-ANL Nestor J." {zaluzec-at-aaem.amc.anl.gov} 22, 38 -- Message-Id: {532B52EA-D0E9-4DEB-94F4-4C69F386722B-at-aaem.amc.anl.gov} 22, 38 -- References: {201506151212.t5FCCY5o025941-at-ns.microscopy.com} 22, 38 -- To: frank_karl-at-ardl.com, 22, 38 -- MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 38 -- X-Mailer: Apple Mail (2.1878.6) 22, 38 -- Content-Transfer-Encoding: 8bit 22, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5FD9MZp022326 ==============================End of - Headers==============================
--
"An expert is a person who has made all the mistakes which can be made in a very narrow field.â and "Prediction is very difficult, especially if it's about the future." Niels Bohr
==============================Original Headers============================== 40, 32 -- From microscopy.gmb-at-gmail.com Mon Jun 15 19:53:14 2015 40, 32 -- Received: from mail-wi0-f172.google.com (mail-wi0-f172.google.com [209.85.212.172]) 40, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5G0rDee012326 40, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 19:53:13 -0500 40, 32 -- Received: by wicnd19 with SMTP id nd19so39569092wic.1 40, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 17:53:13 -0700 (PDT) 40, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 40, 32 -- d=gmail.com; s=20120113; 40, 32 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 40, 32 -- :content-type:content-transfer-encoding; 40, 32 -- bh=MY43nQncRuAGQf5ZtC2Kk/hHhCQQIHnqHdvVy2ea8k0=; 40, 32 -- b=iNm8O8Q8pbPlvkVUDHiju+bxLfRBfwI6OF4sr8cruo7dfYnDOrpSpObhNbZbosdppt 40, 32 -- IHmqy+rcfEmGdrpQZh0DfHhpCjNrrDpv3/cCBiPchmXf0CcIjo6eh5Jkf6eQSwcCln5J 40, 32 -- mwssNhCCispclwnHwcxvbyIQWzJxl1rmTT0ptyWJhCPdpk/qB4IsqFtlbxdVyVbFzkP0 40, 32 -- ZRYmlYBu4OtWuoMomQpA4NvRXEB3FrPqBFgwuCFxEFr5mbEH6a6X1qG0btYBqCgWgRli 40, 32 -- D2zFbD/bnE0TQWbiQiG4Z7c8BgqXa8WZre34aejNUgjERPmtvCK3SbzGO99Ye6d6psy/ 40, 32 -- Nyxg== 40, 32 -- MIME-Version: 1.0 40, 32 -- X-Received: by 10.181.29.100 with SMTP id jv4mr475060wid.4.1434415992930; Mon, 40, 32 -- 15 Jun 2015 17:53:12 -0700 (PDT) 40, 32 -- Received: by 10.180.206.239 with HTTP; Mon, 15 Jun 2015 17:53:12 -0700 (PDT) 40, 32 -- In-Reply-To: {201506151325.t5FDPAF4020845-at-ns.microscopy.com} 40, 32 -- References: {201506151325.t5FDPAF4020845-at-ns.microscopy.com} 40, 32 -- Date: Mon, 15 Jun 2015 19:53:12 -0500 40, 32 -- Message-ID: {CADhGOT+XuD_dWNxTfGFMMVsmKgcu5Mvfn0NjeJ_0-ai2XHw1Hg-at-mail.gmail.com} 40, 32 -- Subject: Fwd: [Microscopy] Re: measurements: It's how small? or more 40, 32 -- importantly what is your accuracy and precision.... 40, 32 -- From: Gary Brown {microscopy.gmb-at-gmail.com} 40, 32 -- To: Listserver {Microscopy-at-microscopy.com} , Frank Karl {frank_karl-at-ardl.com} 40, 32 -- Content-Type: text/plain; charset=UTF-8 40, 32 -- Content-Transfer-Encoding: 8bit 40, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5G0rDee012326 ==============================End of - Headers==============================
Gary, I completely concur with your statements below and I think Dale would agree also.
I would only add that NIST is somewhere in the middle of another evaluation of standardless EDS. But 15 years later, the preliminary results *do not* look any more promising than you noted yourself. I posted Dale's slide summarizing these more recent (2011) examples here:
On 6/15/2015 6:02 PM, microscopy.gmb-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Frank, } } On a related point, inaccuracy of standardless "quantitative" EDS } analyses can lead to substantial errors. An approach by Dale Newbury } (Joseph Goldstein, Dale E. Newbury, David C. Joy, Charles E. Lyman, } Patrick Echlin, Eric Lifshin, Linda Sawyer, J.R. Michael, Scanning } Electron Microscopy and X-ray Microanalysis: Third Ed., Springer, } 2012, 355) is that numerical values for concentration of such analyses } are reported as a range. Specifically, the concentration of an } element is represented by one of three categories: Major: } 10 wt%; } Minor: 1-10 wt%; and Trace: { 1 wt%. } } The lab I retired from used this approach to give our customers some } sense of the prevalence of an element in a qualitative analysis. } After acquiring a qualitative EDS spectrum, we ran a quick } standardless quantitative analysis and reported the "concentrations" } as major, minor or trace, as described above. Furthermore, we did not } divulge to our customers the concentration ranges that the three } categories represented. This solved several problems, one of which } was that we did not provide our internal customer a potentially } worthless number that could be used as the final word on concentration } of the element in the sample. My apologies to Dale for any } misstatements. } } All the best, } } Gary Brown } Polymer Microscopy Consultant } } } } ---------- Forwarded message ---------- } X-from: {zaluzec-at-aaem.amc.anl.gov} } Date: Mon, Jun 15, 2015 at 8:25 AM } Subject: [Microscopy] Re: measurements: It's how small? or more } importantly what is your accuracy and precision.... } To: microscopy.gmb-at-gmail.com } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Frank } } I would contend, that you are asking the wrong question. The subject } should be accuracy and precision of a measurement. } } It is not how many digits you report after a measurement, but } more importantly, reporting of the standard error with any measurement } be it dimension, composition or whatever. Both accuracy and precision. } Without an measurement error estimate then the number of digits is irrelevant. } } My 2 cents. } } Nestor } Your Friendly Neighborhood SysOp } } } BTW, I chuckle at seeing measurements reported more than 3 significant figures } and no error bars. } } } On Jun 15, 2015, at 7:12 AM CDT, {frank_karl-at-ardl.com} } {frank_karl-at-ardl.com} wrote: } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Monday, the start of another workweek but I thought I'd ask a question of the collective wisdom. } } } } How many decimal points do you report or believe when making measurements with: } } } } SEM? } } TEM? } } LM? } } } } Do you report numbers suggesting you can measure to one thousandth of a nanometer? How about a hundredth of a micron or micrometer? What about EDS analysis? On a non-flat, non-homogeneous sample can you actually measure to a tenth of 1 wt%? How about hundredth? } } } } } } Will I get a lot of Out-of-Office responses and do I believe them? } } } } Questions on Monday morning to start the gray matter working.\ } } } } Stay safe................ } } Frank } } } } } } } } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } } } } } } ==============================Original Headers============================== } } 13, 28 -- From frank_karl-at-ardl.com Mon Jun 15 07:12:32 2015 } } 13, 28 -- Received: from cal1-mh779.smtproutes.com (cal1-mh779.smtproutes.com [208.70.91.145]) } } 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5FCCWW1025934 } } 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 07:12:32 -0500 } } 13, 28 -- X-Katharion-ID: 1434370330.87475.cal1-mh779 } } 13, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by } } 13, 28 -- cal1-mh779.smtproutes.com [(192.69.16.145)] with ESMTP via TCP } } 13, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 15 Jun 2015 12:12:10 +0000 } } 13, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by } } 13, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 15 } } 13, 28 -- Jun 2015 08:12:10 -0400 } } 13, 28 -- From: Frank Karl {frank_karl-at-ardl.com} } } 13, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" } } 13, 28 -- {microscopy-at-microscopy.com} } } 13, 28 -- Date: Mon, 15 Jun 2015 08:12:09 -0400 } } 13, 28 -- Subject: meassurements: It's how small? } } 13, 28 -- Thread-Topic: meassurements: It's how small? } } 13, 28 -- Thread-Index: AdCnZID1Qkvf8eMpRwutUMpq1aZTdQ== } } 13, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772CBF0B0518-at-exchange2k7.ad.ardl.com} } } 13, 28 -- Accept-Language: en-US } } 13, 28 -- Content-Language: en-US } } 13, 28 -- X-MS-Has-Attach: } } 13, 28 -- X-MS-TNEF-Correlator: } } 13, 28 -- acceptlanguage: en-US } } 13, 28 -- Content-Type: text/plain; charset="us-ascii" } } 13, 28 -- MIME-Version: 1.0 } } 13, 28 -- Content-Transfer-Encoding: 8bit } } 13, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5FCCWW1025934 } } ==============================End of - Headers============================== } =========================================== } Dr. Nestor J. Zaluzec } Argonne National Laboratory } Electron Microscopy Center } NanoScience and Technology Division } 9700 S. Cass Ave } Bldg 212 / A-143 } Argonne, Illinois 60439 USA } Email: Zaluzec-at-aaem.amc.anl.gov } } } Tel: 530-NES-TORZ (530-637-8679) has Voice Mail } Lab: 630-252-7901 } Fax: 630-252-4798 } } iChat: Zaluzec-at-AIM.com } Skype: Zaluzec } Polycom: 146.139.72.119 } TPM: http://tpm.amc.anl.gov } } } Senior Scientist - Argonne National Laboratory } Fellow of the Microscopy Society of America } Senior Fellow the Computational Institute - University of Chicago } E.P. Wigner Fellow - Oak Ridge National Laboratory } Past President Microscopy Society of America } Adjunct Professor of Physics - Northern Illinois University & } the University of Illinois at Chicago } Visiting Professor of Microscopy - Manchester University } } =========================================== } TPMLab: http://tpm.amc.anl.gov } MMSite: http://www.amc.anl.gov } =========================================== } } The box said ... } "This program requires Win 95/98/NT or better..." } So I bought a Mac ! } } =========================================== } LLAP } =========================================+===== } } } } ==============================Original Headers============================== } 22, 38 -- From anl.nestor.zaluzec-at-gmail.com Mon Jun 15 08:09:23 2015 } 22, 38 -- Received: from mail-ie0-f178.google.com } (mail-ie0-f178.google.com [209.85.223.178]) } 22, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id t5FD9MZp022326 } 22, 38 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 08:09:22 -0500 } 22, 38 -- Received: by iebmu5 with SMTP id mu5so61845784ieb.1 } 22, 38 -- for {microscopy-at-microscopy.com} ; Mon, 15 Jun 2015 } 06:09:22 -0700 (PDT) } 22, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 22, 38 -- d=gmail.com; s=20120113; } 22, 38 -- } h=sender:content-type:mime-version:subject:from:in-reply-to:date:cc } 22, 38 -- :content-transfer-encoding:message-id:references:to; } 22, 38 -- bh=iPg42v6KTKE6jTY1aGXtqkJoEBTPNUaBB8AGEeu7wAo=; } 22, 38 -- } b=Du0htZv5I5L+gPPxzkvE1hcde2t6wDIr26o4bmTBdaJ26xykr0sUwG7Vx2EEDd9cpu } 22, 38 -- } ClaY5KB2BgC/LqMXFAaKG0CE6t1OK+svasMbmbWXDhecsPgcEvG50YXrzNkmXo+1KFni } 22, 38 -- } ZQ0gmAkCGz8bQaQbnLq6ketYYz0neDuQ2YnrAooU/6R007ezI5FdNnEqwTvK7sC73Sjn } 22, 38 -- } iysN0MyZSDJq60E9CeYb8Fq7Se5Vi85QLkqzKZ0hN+oO61xmvHyfRmNj1a4YnlbR+CaX } 22, 38 -- } yNNQ/koyh7xqOMlw2yo7Z9y+QNuvXIYWv9eEEjImbZMUVH0q01pZQrj5Og5DwS4nnJiy } 22, 38 -- Slpw== } 22, 38 -- X-Received: by 10.107.31.134 with SMTP id } f128mr34329165iof.19.1434373762350; } 22, 38 -- Mon, 15 Jun 2015 06:09:22 -0700 (PDT) } 22, 38 -- Received: from [10.0.0.6] ([67.176.143.24]) } 22, 38 -- by mx.google.com with ESMTPSA id } 82sm1556216ioh.3.2015.06.15.06.09.20 } 22, 38 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); } 22, 38 -- Mon, 15 Jun 2015 06:09:21 -0700 (PDT) } 22, 38 -- Sender: "ANL.Nestor Zaluzec" {anl.nestor.zaluzec-at-gmail.com} } 22, 38 -- Content-Type: text/plain; charset=us-ascii } 22, 38 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.6\)) } 22, 38 -- Subject: Re: [Microscopy] measurements: It's how small? or } more importantly what is your accuracy and precision.... } 22, 38 -- From: "Nestor J. Zaluzec - ANL/GAccnt" {zaluzec-at-aaem.amc.anl.gov} } 22, 38 -- In-Reply-To: {201506151212.t5FCCY5o025941-at-ns.microscopy.com} } 22, 38 -- Date: Mon, 15 Jun 2015 08:09:20 -0500 } 22, 38 -- Cc: "Zaluzec-ANL Nestor J." {zaluzec-at-aaem.amc.anl.gov} } 22, 38 -- Message-Id: {532B52EA-D0E9-4DEB-94F4-4C69F386722B-at-aaem.amc.anl.gov} } 22, 38 -- References: {201506151212.t5FCCY5o025941-at-ns.microscopy.com} } 22, 38 -- To: frank_karl-at-ardl.com, } 22, 38 -- MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 22, 38 -- X-Mailer: Apple Mail (2.1878.6) } 22, 38 -- Content-Transfer-Encoding: 8bit } 22, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id t5FD9MZp022326 } ==============================End of - Headers============================== } } }
-- John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1443 E. 13th Ave (541) 346-4655 (probe) Eugene, OR (541) 346-6854 (FAX) 97403-1241
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From mike.sfsd4f564s6df45dsnialh-at-gmail.com Tue Jun 16 17:27:56 2015 Return-Path: {mike.sfsd4f564s6df45dsnialh-at-gmail.com} Received: from gmail.com ([61.100.180.52]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t5GMRp5d019453 for {microscopylistserverarchive5-at-microscopy.com} ; Tue, 16 Jun 2015 17:27:55 -0500 Message-ID: {D8731FA8.4C202FA8-at-gmail.com}
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Email: smodla-at-udel.edu Name: Shannon Modla
Organization: University of Delaware
Title-Subject: [Filtered] Microwave processor
Message: We recently acquired a Pelco BioWave Microwave processor and a Pelco SteadyTemp. Unfortunately, the unit did not come with the connectors or tubing needed to connect the SteadyTemp to the microwave processor. Looking at the back of the SteadyTemp, there are two threaded ports. It looks like there should be a connector to attach the tubing to the threaded ports. I was wondering if anyone who has this unit might know what type of connectors we would need to purchase to get the unit up and running.
Thanks, Shannon
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==============================Original Headers============================== 12, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Jun 16 22:12:43 2015 12, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5H3CgnZ023743 12, 17 -- for {microscopy-at-microscopy.com} ; Tue, 16 Jun 2015 22:12:42 -0500 12, 17 -- Message-ID: {5580E5AA.6060709-at-microscopy.com} 12, 17 -- Date: Tue, 16 Jun 2015 22:12:42 -0500 12, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 12, 17 -- MIME-Version: 1.0 12, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 17 -- Subject: viaWWW:Microwave processor 12, 17 -- References: {201506161805.t5GI5ZC8024833-at-ns.microscopy.com} 12, 17 -- In-Reply-To: {201506161805.t5GI5ZC8024833-at-ns.microscopy.com} 12, 17 -- X-Forwarded-Message-Id: {201506161805.t5GI5ZC8024833-at-ns.microscopy.com} 12, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Don't forget the deadline for early registration for the 2015 Microscopy and Microanalysis meetings in Portland is next week (June 22).
There is still space for a few more student bursaries* to help with meeting activities such as provide support in the different symposia, staff the volunteer office, monitor use of the Internet Café, and help with vendor tutorial sign-up. MSA's student bursary program is designed to encourage students to attend the meetings by helping to defray some of the costs and giving them an opportunity to meet and interact with the established microscopy community.
Bursaries will earn $10/hour for assisting with any of the tasks mentioned above (paid by check at the end of the meetings). There is an added bonus of a meeting shirt and $10 cash for each morning and/or afternoon session worked to help with meals. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.
*For those 'non-students' volunteers are also needed to help with these same meeting activities. Although not paid on an hourly basis as the student bursaries, they do receive a meeting shirt and the same cash allotment to help with meals. Volunteers also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
We can't do it without help from you, so please consider serving as a student bursary or volunteer. If anyone has any questions about the bursary/volunteer program, and/or would like to participate, please contact me.
Thanks and see you in Portland! Amanda
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University Box 9775 Mississippi State, MS 39762
==============================Original Headers============================== 18, 31 -- From ALawrence-at-i2at.msstate.edu Wed Jun 17 06:04:43 2015 18, 31 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 18, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5HB4heM027486 18, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2015 06:04:43 -0500 18, 31 -- Received: from mail03.ad.msstate.edu (mail03.ad.msstate.edu [130.18.230.62]) 18, 31 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id t5HB4g8L015372 18, 31 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 18, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2015 06:04:42 -0500 18, 31 -- X-Sender: {} 18, 31 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by 18, 31 -- mail03.ad.msstate.edu (2002:8212:e63e::8212:e63e) with Microsoft SMTP Server 18, 31 -- (TLS) id 15.0.913.22; Wed, 17 Jun 2015 06:04:42 -0500 18, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 18, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 18, 31 -- 15.00.0913.011; Wed, 17 Jun 2015 06:04:30 -0500 18, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 18, 31 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 18, 31 -- Subject: M&M 2015 Portland Meeting Assistance 18, 31 -- Thread-Topic: M&M 2015 Portland Meeting Assistance 18, 31 -- Thread-Index: AdCo6+tKnj8Z5KiVQY+Tr5ajC/LwBw== 18, 31 -- Date: Wed, 17 Jun 2015 11:04:29 +0000 18, 31 -- Message-ID: {3201e4b009a343969cf470be975b367e-at-mail02.ad.msstate.edu} 18, 31 -- Accept-Language: en-US 18, 31 -- Content-Language: en-US 18, 31 -- X-MS-Has-Attach: 18, 31 -- X-MS-TNEF-Correlator: 18, 31 -- x-originating-ip: [130.18.230.93] 18, 31 -- Content-Type: text/plain; charset="iso-8859-1" 18, 31 -- MIME-Version: 1.0 18, 31 -- Content-Transfer-Encoding: 8bit 18, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5HB4heM027486 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dspc-at-gmail.com Wed Jun 17 10:00:54 2015 Return-Path: {mike.sfsd4f564s6df45dspc-at-gmail.com} Received: from gmail.com ([118.34.243.91]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t5HF0pra028076 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 17 Jun 2015 10:00:53 -0500 Message-ID: {4AEF1C7D.FEAE1E63-at-gmail.com}
Hey I was wondering if anyone has ever come up with a good repeatable technique for making high dynamic range (HDR) images using SEM or TEM micrographs. I think this could be a useful technique to use on low contrast samples and just to make a cool image. But I can't seem to get it to work on my own. Just wondering if anyone else ever has. Thanks.
ERiC Jay Miller Microscopy & Imaging Specialist Electron Probe Instrumentation Center
Northwestern University Mail: 2036 Cook Hall Office: 1152 Cook Hall 2220 Campus Drive Evanston, IL 60208-3108
ph: (847) 467-0789
http://www.nuance.northwestern.edu
==============================Original Headers============================== 9, 49 -- From eric-miller-at-northwestern.edu Wed Jun 17 17:06:43 2015 9, 49 -- Received: from evcspsym1.ads.northwestern.edu (evcspsym1.ads.northwestern.edu [129.105.238.5]) 9, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5HM6hqj008489 9, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2015 17:06:43 -0500 9, 49 -- X-AuditID: 8169ee05-f79f36d000004870-30-5581ef72da0b 9, 49 -- Received: from evcspmbx04.ads.northwestern.edu (evcspmbx04.ads.northwestern.edu [165.124.43.176]) 9, 49 -- by evcspsym1.ads.northwestern.edu (Symantec Messaging Gateway) with SMTP id 21.DE.18544.27FE1855; Wed, 17 Jun 2015 17:06:42 -0500 (CDT) 9, 49 -- Received: from EVCSPMBX01.ads.northwestern.edu (165.124.43.173) by 9, 49 -- evcspmbx04.ads.northwestern.edu (165.124.43.176) with Microsoft SMTP Server 9, 49 -- (TLS) id 15.0.1076.9; Wed, 17 Jun 2015 17:06:42 -0500 9, 49 -- Received: from EVCSPMBX01.ads.northwestern.edu ([fe80::431:8478:d25e:21c7]) by 9, 49 -- evcspmbx01.ads.northwestern.edu ([fe80::431:8478:d25e:21c7%13]) with mapi id 9, 49 -- 15.00.1076.000; Wed, 17 Jun 2015 17:06:42 -0500 9, 49 -- From: Eric Jay Miller {eric-miller-at-northwestern.edu} 9, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 49 -- Subject: High Dynamic Range Images for EM? 9, 49 -- Thread-Topic: High Dynamic Range Images for EM? 9, 49 -- Thread-Index: AdCpSZ1ifIkrlfjgSy6LullTFNvYtQ== 9, 49 -- Date: Wed, 17 Jun 2015 22:06:41 +0000 9, 49 -- Message-ID: {24cc1af57a124b89a6af3943470f1110-at-evcspmbx01.ads.northwestern.edu} 9, 49 -- Accept-Language: en-US 9, 49 -- Content-Language: en-US 9, 49 -- X-MS-Has-Attach: 9, 49 -- X-MS-TNEF-Correlator: 9, 49 -- x-ms-exchange-transport-fromentityheader: Hosted 9, 49 -- x-originating-ip: [129.105.136.8] 9, 49 -- Content-Type: text/plain; charset="us-ascii" 9, 49 -- MIME-Version: 1.0 9, 49 -- X-Brightmail-Tracker: H4sIAAAAAAAAA02Tf2wTZRjH87bXchu97XZdt4eqRJuIuLEOJgaNVkdiImoyR6QaNBFv67l2 9, 49 -- 69py15bNqGmdSlbYDyALsJlaYW2AIM5FzCzLSLpMoaJjOpQNBYJlOroxxeA6oIt3vXa9/573 9, 49 -- 87zf7/O998nhcqotR4tbbE6GtdFWnTIXC75b2lfGznmNa0fjTz5xb9izrBJt+mdXXFaNXs99 9, 49 -- 2sRYLW6GLX/mrVzzDx92IUcSa7rbGZJ50ILch3JwINfD8Kl9SrEugvOXv+DrXJwi4wjif04i 9, 49 -- 8XAGwaG+iFw8jCI4sn8SEyRKXp4c6+ElOF5IPgUj/QYBq8kS6PN7MRGXQ2hipYALST180NOV 9, 49 -- UmLkw9Ax7E8NJsgqONM6nuKIDzEfPS4TajlZDJOxT2ViOBJ6B0fToTUw/ceiQqzXwsngECbW 9, 49 -- D8G5rw8qRO0aCJy6pRTrUgh9FpeLswrg7MEY1ok03ZIR3RJJt0TSLZEEEHYMrWTctZyDa25c 9, 49 -- p6dNnN5mZ53mHQwn7EPPmFz9iF+J13JTMYA69pVFEIkjnYrwDHiMlIJ287IIegmX6TTEspte 9, 49 -- I5VXYzc1m2nOvI11WRlOV0jkCZhYwjUua4NOS3wkUPUStTE7OCvj5GdGEOByXma4JshMdPM7 9, 49 -- DGsXzSLoPhzTFRPz09uNFFlHO5kGhnEwbKa7Bcd1QPTN8sIClqljmt62WJ2ZNq9LxvkOKe2k 9, 49 -- wjxAPBjmG0XShjSPDM+JoE24ig81JXgTnINu5Cx1aV81UXOdp6oMTXmuIDYKw6gMzPpFUS0e 9, 49 -- SFz6XEZhNruN0RYTpwVTUrhpdtmW8mqLCLf3fSOVL2kI1tr7iV1f8W+vkfCse+b3uYFmEb8n 9, 49 -- NVEtvLOK/7uygSkiLExcnoapvEDsnBGeLc2yhhVB3oe8J4cTkz45LJ7tUMBM4BAB7b2teXAg 9, 49 -- cYCEzraJAoglZzRweY9vFXT5jz8Cv8XmV8NfFwdLIeEZWAMDgz16aP15qgJ6QwEDXAzOPge7 9, 49 -- Z/c+D3e+W3gRWsajVeAfvLYZhkK/vgJHpsNGWBg7/RpMXQ29Abvbrm6D8R8P18KViY8bwL/4 9, 49 -- iRUu9IcdkOgfcULv0VgTLMyNvHeD35CM35BJeDOCc9JO6YYsAlVlaHpDqatUBmY/WetB+mR9 9, 49 -- UjN0skURXV2mbK8/Wv8NnQMvd26k3Hcrb31fZKpiId/HnX/h3783uB7dUP946+GSzbeD5dM1 9, 49 -- Y3nVzp3LA29+O/zTiL3ixLHtW+0Fd7b+V+m7goXtl+YMr0Yhkb9qy4rGBF6357GW/RfoqnZs 9, 49 -- ruPZ279E18u+nDDMJ6Z+v35OrcM4M72uRM5y9P/ChWqoIAUAAA== 9, 49 -- Content-Transfer-Encoding: 8bit 9, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5HM6hqj008489 ==============================End of - Headers==============================
Try a Y-modulation scan in the SEM (or SEM mode of a STEM). Basically a line-profile aka Y-scan that is scanned down an image. Ends up looking line a bas relief. The more scan lines, the finer the image. The advantage is that since contrast is in the "Y" direction on the monitor - the line profile - one can often crank the contrast more than with a normal XY scan. Just watch for peak clipping indicating saturation. (This is an old method, dating to at least 1969. Maybe older. But oddly enough, this is difficult to do with some modern SEMs' software.)
Phil
} Hey I was wondering if anyone has ever come up with a good repeatable technique for making high dynamic range (HDR) images using SEM or TEM micrographs. I think this could be a useful technique to use on low contrast samples and just to make a cool image. But I can't seem to get it to work on my own. Just wondering if anyone else ever has. Thanks. } } } } ERiC Jay Miller } Microscopy& Imaging Specialist } Electron Probe Instrumentation Center } } Northwestern University } Mail: 2036 Cook Hall } Office: 1152 Cook Hall } 2220 Campus Drive } Evanston, IL 60208-3108 } } ph: (847) 467-0789 } } http://www.nuance.northwestern.edu -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 32 -- From oshel1pe-at-cmich.edu Thu Jun 18 07:08:28 2015 4, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5IC8Swf015349 4, 32 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 07:08:28 -0500 4, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 4, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t5IC8LNd020460; 4, 32 -- Thu, 18 Jun 2015 08:08:25 -0400 4, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 4, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 4, 32 -- (TLS) id 14.3.195.1; Thu, 18 Jun 2015 08:08:23 -0400 4, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 32 -- 14.3.195.1; Thu, 18 Jun 2015 08:08:23 -0400 4, 32 -- Message-ID: {5582B4B0.5050507-at-cmich.edu} 4, 32 -- Date: Thu, 18 Jun 2015 08:08:16 -0400 4, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 32 -- MIME-Version: 1.0 4, 32 -- To: {eric-miller-at-northwestern.edu} , micro {microscopy-at-microscopy.com} 4, 32 -- Subject: Re: [Microscopy] High Dynamic Range Images for EM? 4, 32 -- References: {201506172224.t5HMOCrb026140-at-ns.microscopy.com} 4, 32 -- In-Reply-To: {201506172224.t5HMOCrb026140-at-ns.microscopy.com} 4, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 32 -- Content-Transfer-Encoding: 7bit 4, 32 -- X-Originating-IP: [141.209.2.100] 4, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 32 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 4, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 4, 32 -- X-CanItPRO-Stream: default 4, 32 -- X-Canit-Stats-ID: 02OFM8ppC - 888d92d81121 - 20150618 4, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Hi, There are two issues, it seems: pixel depth and look-up table. I was a little confused by your statement of low contrast images, for HDR is usually used for high contrast images, where some places are saturated and/or some are lost in the black. That issue beside, with an 8-bit detector signal, one could take two images - at lower and higher gain - and then map the two onto a single 16 or 32 bit image. Then the gray levels need to be compressed into a non-linear scale that allows the wide range to show in a human-detectable fashion. A method used in astronomy is the wavelet-log representation, for example, where the pixel values are converted to a log scale. This allows many orders of magnitude of signal to be represented in a nice way visually. See Starck and Bobin, Astronomical Data Analysis, 2009, Equation (2). Of course, you must start with a high bit-depth image. I wrote an ImageJ macro that does such a wavelet transform, and my meager testing found it to be good for bringing out faint details in 32-bit STEM images. Low contrast images don't look good, for they get compressed into too few gray levels. I would be glad to send it to you offline, as the listserver doesn't digest attachments. Regards, Larry Scipioni ZS Genetics
-----Original Message----- X-from: eric-miller-at-northwestern.edu [mailto:eric-miller-at-northwestern.edu] Sent: Wednesday, June 17, 2015 6:23 PM To: LES-at-ZSGENETICS.COM
Hey I was wondering if anyone has ever come up with a good repeatable technique for making high dynamic range (HDR) images using SEM or TEM micrographs. I think this could be a useful technique to use on low contrast samples and just to make a cool image. But I can't seem to get it to work on my own. Just wondering if anyone else ever has. Thanks.
ERiC Jay Miller Microscopy & Imaging Specialist Electron Probe Instrumentation Center
Northwestern University Mail: 2036 Cook Hall Office: 1152 Cook Hall 2220 Campus Drive Evanston, IL 60208-3108
ph: (847) 467-0789
http://www.nuance.northwestern.edu
==============================Original Headers============================== 9, 49 -- From eric-miller-at-northwestern.edu Wed Jun 17 17:06:43 2015 9, 49 -- Received: from evcspsym1.ads.northwestern.edu (evcspsym1.ads.northwestern.edu [129.105.238.5]) 9, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5HM6hqj008489 9, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2015 17:06:43 -0500 9, 49 -- X-AuditID: 8169ee05-f79f36d000004870-30-5581ef72da0b 9, 49 -- Received: from evcspmbx04.ads.northwestern.edu (evcspmbx04.ads.northwestern.edu [165.124.43.176]) 9, 49 -- by evcspsym1.ads.northwestern.edu (Symantec Messaging Gateway) with SMTP id 21.DE.18544.27FE1855; Wed, 17 Jun 2015 17:06:42 -0500 (CDT) 9, 49 -- Received: from EVCSPMBX01.ads.northwestern.edu (165.124.43.173) by 9, 49 -- evcspmbx04.ads.northwestern.edu (165.124.43.176) with Microsoft SMTP Server 9, 49 -- (TLS) id 15.0.1076.9; Wed, 17 Jun 2015 17:06:42 -0500 9, 49 -- Received: from EVCSPMBX01.ads.northwestern.edu ([fe80::431:8478:d25e:21c7]) by 9, 49 -- evcspmbx01.ads.northwestern.edu ([fe80::431:8478:d25e:21c7%13]) with mapi id 9, 49 -- 15.00.1076.000; Wed, 17 Jun 2015 17:06:42 -0500 9, 49 -- From: Eric Jay Miller {eric-miller-at-northwestern.edu} 9, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 49 -- Subject: High Dynamic Range Images for EM? 9, 49 -- Thread-Topic: High Dynamic Range Images for EM? 9, 49 -- Thread-Index: AdCpSZ1ifIkrlfjgSy6LullTFNvYtQ== 9, 49 -- Date: Wed, 17 Jun 2015 22:06:41 +0000 9, 49 -- Message-ID: {24cc1af57a124b89a6af3943470f1110-at-evcspmbx01.ads.northwestern.edu} 9, 49 -- Accept-Language: en-US 9, 49 -- Content-Language: en-US 9, 49 -- X-MS-Has-Attach: 9, 49 -- X-MS-TNEF-Correlator: 9, 49 -- x-ms-exchange-transport-fromentityheader: Hosted 9, 49 -- x-originating-ip: [129.105.136.8] 9, 49 -- Content-Type: text/plain; charset="us-ascii" 9, 49 -- MIME-Version: 1.0 9, 49 -- X-Brightmail-Tracker: H4sIAAAAAAAAA02Tf2wTZRjH87bXchu97XZdt4eqRJuIuLEOJgaNVkdiImoyR6QaNBFv67l2 9, 49 -- 69py15bNqGmdSlbYDyALsJlaYW2AIM5FzCzLSLpMoaJjOpQNBYJlOroxxeA6oIt3vXa9/573 9, 49 -- 87zf7/O998nhcqotR4tbbE6GtdFWnTIXC75b2lfGznmNa0fjTz5xb9izrBJt+mdXXFaNXs99 9, 49 -- 2sRYLW6GLX/mrVzzDx92IUcSa7rbGZJ50ILch3JwINfD8Kl9SrEugvOXv+DrXJwi4wjif04i 9, 49 -- 8XAGwaG+iFw8jCI4sn8SEyRKXp4c6+ElOF5IPgUj/QYBq8kS6PN7MRGXQ2hipYALST180NOV 9, 49 -- UmLkw9Ax7E8NJsgqONM6nuKIDzEfPS4TajlZDJOxT2ViOBJ6B0fToTUw/ceiQqzXwsngECbW 9, 49 -- D8G5rw8qRO0aCJy6pRTrUgh9FpeLswrg7MEY1ok03ZIR3RJJt0TSLZEEEHYMrWTctZyDa25c 9, 49 -- p6dNnN5mZ53mHQwn7EPPmFz9iF+J13JTMYA69pVFEIkjnYrwDHiMlIJ287IIegmX6TTEspte 9, 49 -- I5VXYzc1m2nOvI11WRlOV0jkCZhYwjUua4NOS3wkUPUStTE7OCvj5GdGEOByXma4JshMdPM7 9, 49 -- DGsXzSLoPhzTFRPz09uNFFlHO5kGhnEwbKa7Bcd1QPTN8sIClqljmt62WJ2ZNq9LxvkOKe2k 9, 49 -- wjxAPBjmG0XShjSPDM+JoE24ig81JXgTnINu5Cx1aV81UXOdp6oMTXmuIDYKw6gMzPpFUS0e 9, 49 -- SFz6XEZhNruN0RYTpwVTUrhpdtmW8mqLCLf3fSOVL2kI1tr7iV1f8W+vkfCse+b3uYFmEb8n 9, 49 -- NVEtvLOK/7uygSkiLExcnoapvEDsnBGeLc2yhhVB3oe8J4cTkz45LJ7tUMBM4BAB7b2teXAg 9, 49 -- cYCEzraJAoglZzRweY9vFXT5jz8Cv8XmV8NfFwdLIeEZWAMDgz16aP15qgJ6QwEDXAzOPge7 9, 49 -- Z/c+D3e+W3gRWsajVeAfvLYZhkK/vgJHpsNGWBg7/RpMXQ29Abvbrm6D8R8P18KViY8bwL/4 9, 49 -- iRUu9IcdkOgfcULv0VgTLMyNvHeD35CM35BJeDOCc9JO6YYsAlVlaHpDqatUBmY/WetB+mR9 9, 49 -- UjN0skURXV2mbK8/Wv8NnQMvd26k3Hcrb31fZKpiId/HnX/h3783uB7dUP946+GSzbeD5dM1 9, 49 -- Y3nVzp3LA29+O/zTiL3ixLHtW+0Fd7b+V+m7goXtl+YMr0Yhkb9qy4rGBF6357GW/RfoqnZs 9, 49 -- ruPZ279E18u+nDDMJ6Z+v35OrcM4M72uRM5y9P/ChWqoIAUAAA== 9, 49 -- Content-Transfer-Encoding: 8bit 9, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5HM6hqj008489 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 45 -- From les-at-zsgenetics.com Thu Jun 18 07:46:29 2015 17, 45 -- Received: from gateway05.websitewelcome.com (gateway05.websitewelcome.com [69.93.164.10]) 17, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5ICkS1D003817 17, 45 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 07:46:28 -0500 17, 45 -- Received: by gateway05.websitewelcome.com (Postfix, from userid 5007) 17, 45 -- id E121D9B08575A; Thu, 18 Jun 2015 07:46:27 -0500 (CDT) 17, 45 -- Received: from gator2013.hostgator.com (gator2013.hostgator.com [50.87.144.13]) 17, 45 -- by gateway05.websitewelcome.com (Postfix) with ESMTP id C93ED9B08573A 17, 45 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 07:46:27 -0500 (CDT) 17, 45 -- Received: from [146.115.5.114] (port=56417 helo=LESZSG) 17, 45 -- by gator2013.hostgator.com with esmtpsa (TLSv1:AES256-SHA:256) 17, 45 -- (Exim 4.82) 17, 45 -- (envelope-from {les-at-zsgenetics.com} ) 17, 45 -- id 1Z5ZD9-00076C-Ag; Thu, 18 Jun 2015 07:46:27 -0500 17, 45 -- From: "Larry Scipioni" {les-at-zsgenetics.com} 17, 45 -- To: {eric-miller-at-northwestern.edu} 17, 45 -- Cc: {microscopy-at-microscopy.com} 17, 45 -- References: {201506172223.t5HMNJm9025217-at-ns.microscopy.com} 17, 45 -- In-Reply-To: {201506172223.t5HMNJm9025217-at-ns.microscopy.com} 17, 45 -- Subject: RE: [Microscopy] High Dynamic Range Images for EM? 17, 45 -- Date: Thu, 18 Jun 2015 08:46:27 -0400 17, 45 -- Organization: ZS Genetics 17, 45 -- Message-ID: {009501d0a9c4$cb5e8d80$621ba880$-at-zsgenetics.com} 17, 45 -- MIME-Version: 1.0 17, 45 -- Content-Type: text/plain; 17, 45 -- charset="us-ascii" 17, 45 -- Content-Transfer-Encoding: 7bit 17, 45 -- X-Mailer: Microsoft Outlook 14.0 17, 45 -- Thread-Index: AQCzU401d7eABzTzAhlMQXEs75YRDZ/s3aOg 17, 45 -- Content-Language: en-us 17, 45 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 17, 45 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com 17, 45 -- X-AntiAbuse: Original Domain - microscopy.com 17, 45 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 17, 45 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com 17, 45 -- X-BWhitelist: no 17, 45 -- X-Source-IP: 146.115.5.114 17, 45 -- X-Exim-ID: 1Z5ZD9-00076C-Ag 17, 45 -- X-Source: 17, 45 -- X-Source-Args: 17, 45 -- X-Source-Dir: 17, 45 -- X-Source-Sender: (LESZSG) [146.115.5.114]:56417 17, 45 -- X-Source-Auth: les-at-zsgenetics.com 17, 45 -- X-Email-Count: 6 17, 45 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20= ==============================End of - Headers==============================
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Message: I would advise that you contact Ted Pella, their number is on their website, www.tedpella.com, and let their customer service know what happened. I am sure they can direct you to the correct hoses and fitting for your units. We just ordered another complete Pella Microwave system and enjoy their products immensely.
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==============================Original Headers============================== 12, 43 -- From eschumacher-at-mccrone.com Thu Jun 18 08:37:49 2015 12, 43 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 12, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5IDbnQr029053 12, 43 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 08:37:49 -0500 12, 43 -- X-ASG-Debug-ID: 1434634668-07bbd429fb0d5e0001-4CH8be 12, 43 -- Received: from TMGEX1.tmg.mccrone.com ([192.168.101.73]) by spam.mccrone.com with ESMTP id BFA6V2rFXYFH61uj for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 08:37:48 -0500 (CDT) 12, 43 -- X-Barracuda-Envelope-From: eschumacher-at-mccrone.com 12, 43 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by 12, 43 -- TMGEX1.tmg.mccrone.com (192.168.101.73) with Microsoft SMTP Server (TLS) id 12, 43 -- 15.0.913.22; Thu, 18 Jun 2015 08:37:47 -0500 12, 43 -- Received: from TMGEX1.tmg.mccrone.com ([::1]) by TMGEX1.tmg.mccrone.com 12, 43 -- ([fe80::e15d:262f:24ef:342a%14]) with mapi id 15.00.0913.011; Thu, 18 Jun 12, 43 -- 2015 08:37:47 -0500 12, 43 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 12, 43 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 12, 43 -- CC: "Gina C. Schuster" {gschuster-at-mccrone.com} 12, 43 -- Subject: Job Opportunity: Electron Optics 12, 43 -- Thread-Topic: Job Opportunity: Electron Optics 12, 43 -- X-ASG-Orig-Subj: Job Opportunity: Electron Optics 12, 43 -- Thread-Index: AdCpywwWq8G58SA5RGOT1FHbzecoTg== 12, 43 -- Date: Thu, 18 Jun 2015 13:37:46 +0000 12, 43 -- Message-ID: {fe9d00c9fe3944ad8cc2776ecdf75130-at-TMGEX1.tmg.mccrone.com} 12, 43 -- Accept-Language: en-US 12, 43 -- Content-Language: en-US 12, 43 -- X-MS-Has-Attach: 12, 43 -- X-MS-TNEF-Correlator: 12, 43 -- x-vipre-scanned: 0FCCF66200A1180FCCF7AF 12, 43 -- x-originating-ip: [192.168.101.85] 12, 43 -- Content-Type: text/plain; charset="iso-8859-1" 12, 43 -- MIME-Version: 1.0 12, 43 -- X-Barracuda-Connect: UNKNOWN[192.168.101.73] 12, 43 -- X-Barracuda-Start-Time: 1434634668 12, 43 -- X-Barracuda-URL: https://spam.mccrone.com:443/cgi-mod/mark.cgi 12, 43 -- X-Virus-Scanned: by bsmtpd at mccrone.com 12, 43 -- X-Barracuda-BRTS-Status: 1 12, 43 -- X-Barracuda-Spam-Score: 0.00 12, 43 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 12, 43 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.19953 12, 43 -- Rule breakdown below 12, 43 -- pts rule name description 12, 43 -- ---- ---------------------- -------------------------------------------------- 12, 43 -- Content-Transfer-Encoding: 8bit 12, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5IDbnQr029053 ==============================End of - Headers==============================
Dear Eric, we did this for TEM some time ago and finally got round to publishing a little letter on it last year: see Microscopy and Microanalysis 20 1601-4 (2014), DOI: 10.1017/S1431927614012975. Also at https://www.researchgate.net/publication/266246078_High_dynamic_range_electron_imaging_the_new_standard. You should be able to find the Digital Micrograph script there too, under supplementary resources.
It doesn't help with low contrast samples, but it does help with very high contrast data (e.g. diffraction patterns). For low contrast samples it would be better to use a phase plate ($$$) or a through-focal series to produce an exit wave reconstruction (or in-line holography, however you want to label it).
Best regards
Richard
On 17/06/2015 23:15, eric-miller-at-northwestern.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hey I was wondering if anyone has ever come up with a good repeatable technique for making high dynamic range (HDR) images using SEM or TEM micrographs. I think this could be a useful technique to use on low contrast samples and just to make a cool image. But I can't seem to get it to work on my own. Just wondering if anyone else ever has. Thanks. } } } } ERiC Jay Miller } Microscopy & Imaging Specialist } Electron Probe Instrumentation Center } } Northwestern University } Mail: 2036 Cook Hall } Office: 1152 Cook Hall } 2220 Campus Drive } Evanston, IL 60208-3108 } } ph: (847) 467-0789 } } http://www.nuance.northwestern.edu } } } } ==============================Original Headers============================== } 9, 49 -- From eric-miller-at-northwestern.edu Wed Jun 17 17:06:43 2015 } 9, 49 -- Received: from evcspsym1.ads.northwestern.edu (evcspsym1.ads.northwestern.edu [129.105.238.5]) } 9, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5HM6hqj008489 } 9, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Jun 2015 17:06:43 -0500 } 9, 49 -- X-AuditID: 8169ee05-f79f36d000004870-30-5581ef72da0b } 9, 49 -- Received: from evcspmbx04.ads.northwestern.edu (evcspmbx04.ads.northwestern.edu [165.124.43.176]) } 9, 49 -- by evcspsym1.ads.northwestern.edu (Symantec Messaging Gateway) with SMTP id 21.DE.18544.27FE1855; Wed, 17 Jun 2015 17:06:42 -0500 (CDT) } 9, 49 -- Received: from EVCSPMBX01.ads.northwestern.edu (165.124.43.173) by } 9, 49 -- evcspmbx04.ads.northwestern.edu (165.124.43.176) with Microsoft SMTP Server } 9, 49 -- (TLS) id 15.0.1076.9; Wed, 17 Jun 2015 17:06:42 -0500 } 9, 49 -- Received: from EVCSPMBX01.ads.northwestern.edu ([fe80::431:8478:d25e:21c7]) by } 9, 49 -- evcspmbx01.ads.northwestern.edu ([fe80::431:8478:d25e:21c7%13]) with mapi id } 9, 49 -- 15.00.1076.000; Wed, 17 Jun 2015 17:06:42 -0500 } 9, 49 -- From: Eric Jay Miller {eric-miller-at-northwestern.edu} } 9, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 9, 49 -- Subject: High Dynamic Range Images for EM? } 9, 49 -- Thread-Topic: High Dynamic Range Images for EM? } 9, 49 -- Thread-Index: AdCpSZ1ifIkrlfjgSy6LullTFNvYtQ== } 9, 49 -- Date: Wed, 17 Jun 2015 22:06:41 +0000 } 9, 49 -- Message-ID: {24cc1af57a124b89a6af3943470f1110-at-evcspmbx01.ads.northwestern.edu} } 9, 49 -- Accept-Language: en-US } 9, 49 -- Content-Language: en-US } 9, 49 -- X-MS-Has-Attach: } 9, 49 -- X-MS-TNEF-Correlator: } 9, 49 -- x-ms-exchange-transport-fromentityheader: Hosted } 9, 49 -- x-originating-ip: [129.105.136.8] } 9, 49 -- Content-Type: text/plain; charset="us-ascii" } 9, 49 -- MIME-Version: 1.0 } 9, 49 -- X-Brightmail-Tracker: H4sIAAAAAAAAA02Tf2wTZRjH87bXchu97XZdt4eqRJuIuLEOJgaNVkdiImoyR6QaNBFv67l2 } 9, 49 -- 69py15bNqGmdSlbYDyALsJlaYW2AIM5FzCzLSLpMoaJjOpQNBYJlOroxxeA6oIt3vXa9/573 } 9, 49 -- 87zf7/O998nhcqotR4tbbE6GtdFWnTIXC75b2lfGznmNa0fjTz5xb9izrBJt+mdXXFaNXs99 } 9, 49 -- 2sRYLW6GLX/mrVzzDx92IUcSa7rbGZJ50ILch3JwINfD8Kl9SrEugvOXv+DrXJwi4wjif04i } 9, 49 -- 8XAGwaG+iFw8jCI4sn8SEyRKXp4c6+ElOF5IPgUj/QYBq8kS6PN7MRGXQ2hipYALST180NOV } 9, 49 -- UmLkw9Ax7E8NJsgqONM6nuKIDzEfPS4TajlZDJOxT2ViOBJ6B0fToTUw/ceiQqzXwsngECbW } 9, 49 -- D8G5rw8qRO0aCJy6pRTrUgh9FpeLswrg7MEY1ok03ZIR3RJJt0TSLZEEEHYMrWTctZyDa25c } 9, 49 -- p6dNnN5mZ53mHQwn7EPPmFz9iF+J13JTMYA69pVFEIkjnYrwDHiMlIJ287IIegmX6TTEspte } 9, 49 -- I5VXYzc1m2nOvI11WRlOV0jkCZhYwjUua4NOS3wkUPUStTE7OCvj5GdGEOByXma4JshMdPM7 } 9, 49 -- DGsXzSLoPhzTFRPz09uNFFlHO5kGhnEwbKa7Bcd1QPTN8sIClqljmt62WJ2ZNq9LxvkOKe2k } 9, 49 -- wjxAPBjmG0XShjSPDM+JoE24ig81JXgTnINu5Cx1aV81UXOdp6oMTXmuIDYKw6gMzPpFUS0e } 9, 49 -- SFz6XEZhNruN0RYTpwVTUrhpdtmW8mqLCLf3fSOVL2kI1tr7iV1f8W+vkfCse+b3uYFmEb8n } 9, 49 -- NVEtvLOK/7uygSkiLExcnoapvEDsnBGeLc2yhhVB3oe8J4cTkz45LJ7tUMBM4BAB7b2teXAg } 9, 49 -- cYCEzraJAoglZzRweY9vFXT5jz8Cv8XmV8NfFwdLIeEZWAMDgz16aP15qgJ6QwEDXAzOPge7 } 9, 49 -- Z/c+D3e+W3gRWsajVeAfvLYZhkK/vgJHpsNGWBg7/RpMXQ29Abvbrm6D8R8P18KViY8bwL/4 } 9, 49 -- iRUu9IcdkOgfcULv0VgTLMyNvHeD35CM35BJeDOCc9JO6YYsAlVlaHpDqatUBmY/WetB+mR9 } 9, 49 -- UjN0skURXV2mbK8/Wv8NnQMvd26k3Hcrb31fZKpiId/HnX/h3783uB7dUP946+GSzbeD5dM1 } 9, 49 -- Y3nVzp3LA29+O/zTiL3ixLHtW+0Fd7b+V+m7goXtl+YMr0Yhkb9qy4rGBF6357GW/RfoqnZs } 9, 49 -- ruPZ279E18u+nDDMJ6Z+v35OrcM4M72uRM5y9P/ChWqoIAUAAA== } 9, 49 -- Content-Transfer-Encoding: 8bit } 9, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5HM6hqj008489 } ==============================End of - Headers==============================
-- Richard Beanland
Director, Integrity Scientific Ltd.
==============================Original Headers============================== 10, 32 -- From contact-at-integrityscientific.com Thu Jun 18 09:12:03 2015 10, 32 -- Received: from mo4-p01-ob.smtp.rzone.de (mo4-p01-ob.smtp.rzone.de [81.169.146.164]) 10, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5IEC2Dh023618 10, 32 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 09:12:02 -0500 10, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; t=1434636721; l=5782; 10, 32 -- s=domk; d=integrityscientific.com; 10, 32 -- h=Content-Transfer-Encoding:Content-Type:In-Reply-To:References: 10, 32 -- Subject:To:MIME-Version:Reply-To:From:Date; 10, 32 -- bh=YMsaJLhONw9mnjoiLFMDBdJFEnDf3hxNEvUCk0cc6Ho=; 10, 32 -- b=PlZxIuJ+R8qXB0z4PiRlLXFOZEvNlt6ZVYa/K/Ipa2O5QgJuoGUKxzQTzpOhtX6jums 10, 32 -- rJTGqXwBe+1sEVWJBrKQdWUw4zD+ZJifU7JQmYZ86dwMSUgZtb29KyREpD0vUGSPZfHQT 10, 32 -- fK70QN2G+A/mkfnuRHjqGeUY1NMGBMuseOE= 10, 32 -- X-RZG-AUTH: :L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBSd5rS69964oI29h2ZepZS93fbcBRZ/xDGCbsBk= 10, 32 -- X-RZG-CLASS-ID: mo01 10, 32 -- Received: from [127.0.0.1] (hosts-137-205-164-152.phys.warwick.ac.uk [137.205.164.152]) 10, 32 -- by smtp.strato.de (RZmta 37.7 AUTH) 10, 32 -- with ESMTPSA id y02947r5IEBwAT4 10, 32 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-SHA (curve X9_62_prime256v1 with 256 ECDH bits, eq. 3072 bits RSA)) 10, 32 -- (Client did not present a certificate); 10, 32 -- Thu, 18 Jun 2015 16:11:58 +0200 (CEST) 10, 32 -- Message-ID: {5582D1AF.7060705-at-integrityscientific.com} 10, 32 -- Date: Thu, 18 Jun 2015 15:11:59 +0100 10, 32 -- From: Richard Beanland {contact-at-integrityscientific.com} 10, 32 -- Reply-To: contact-at-integrityscientific.com 10, 32 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 10, 32 -- MIME-Version: 1.0 10, 32 -- To: eric-miller-at-northwestern.edu, microscopy-at-microscopy.com 10, 32 -- Subject: Re: [Microscopy] High Dynamic Range Images for EM? 10, 32 -- References: {201506172215.t5HMFMur016061-at-ns.microscopy.com} 10, 32 -- In-Reply-To: {201506172215.t5HMFMur016061-at-ns.microscopy.com} 10, 32 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 32 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Thank you all for the suggestions. I confused myself and misspoke when I said low contrast images. Usually when you have an image with very bright or very dark areas in it, you'll need TO TAKE a very low contrast image to make all of the data visible. I just think this is an interesting technique for data as well as artistic reasons that should be able to be applied to EM. Just haven't found an easy way of making it happen yet.
-----Original Message----- X-from: Richard Beanland [mailto:contact-at-integrityscientific.com] Sent: Thursday, June 18, 2015 9:12 AM To: Eric Jay Miller; microscopy-at-microscopy.com
Dear colleagues,
We would like to invite you to a 2nd discussion meeting on open source software for quantitative microanalysis on Tuesday August 4th, 2015 after the second day of the MM2015 conference in Portland, Oregon.
It goes without saying that the microanalysis community heavily depends on software. Quantification software, simulation programs, and physical quantities databases are essential tools for today's microanalysts. Broadly speaking, these software can be classified into four categories: commercial (sold by instrumentation's manufacturers or third-party companies), personal/research group internal or not openly distributed (XFILM, PENELOPE), free to download and use (WinCasino, CalcZAF) and open source (DTSA-II, pyPENELOPE). Without a doubt all types of software play an important role in the community, but we strongly believe that only open source software can foster innovation while offering the same level of validation and transparency as peer-reviewed scientific work. Although closed source software may be based on published equations, they remain black boxes as other equations are sometimes used in running codes. Alternate implementations, further optimization or special cases are not shared with the users. Most importantly, commercial, private and free software are a barrier to innovate as they force scientists, who would like to improve or develop new algorithms, to start from scratch.
We would therefore propose to start a community-driven open source project to centralize physical quantity databases and algorithms used for quantification. The goal is to encourage collaborative work and provide the necessary building blocks for new projects in microanalysis. For more information, you can visit our website openmicroanalysis.org and discussion forum https://groups.google.com/forum/#!forum/openmicroanalysis, where the slides of the 1st meeting held at EMAS2015 are available.
The meeting will take place on: Tuesday August 4th, 2015 at 4:00 to 5:00 PM in room B118 in Oregon Convention Center.
You can gladly forward this invitation to other colleagues that may be interested in joining the discussion.
Best regards, Hendrix, Raynald, Philippe and Silvia
-- ----------------------------------------------------------- Hendrix Demers Research Associate --------------- Department of Mining and Materials Engineering Room 1250 Wong Building 3610 University Street Montreal QC H3A 0C5 Tel: 514-398-4755 ext.0225 Mobile: 514-927-6186 email: hendrix.demers-at-mail.mcgill.ca -----------------------------------------------------------
==============================Original Headers============================== 8, 27 -- From drix00-at-gmail.com Thu Jun 18 11:15:06 2015 8, 27 -- Received: from mail-yk0-f177.google.com (mail-yk0-f177.google.com [209.85.160.177]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5IGF6Kk001377 8, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 11:15:06 -0500 8, 27 -- Received: by ykfl8 with SMTP id l8so70222536ykf.1 8, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 09:15:04 -0700 (PDT) 8, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 27 -- d=gmail.com; s=20120113; 8, 27 -- h=mime-version:date:message-id:subject:from:to:content-type; 8, 27 -- bh=957QdYSjl43iY9123CY92XeDPKa0oda44LO5G8Xu5tI=; 8, 27 -- b=b0jcojroipg40aNbk+zLSdE+h2uYx4ip12Qj002dJZWwKMev8wKIZPkSa4Qyu9058V 8, 27 -- vvQ1e+SEmywopt+fiW3otyOZbuwWgRjYQ5nCTMA8BckJ0Vtauyz9iEH2QDmj8+aven4I 8, 27 -- yYD2OeEB2r75COj1+cAArKnNoAjfqLYOJj28koT4Ni/0i3X8FRHHAgngK6GZotcI8y1w 8, 27 -- AaVOS/PZRaFjO5iZdFt21Yn6II8LybgYr2w6hyL49IxU1p3R/jSwlxpewcMldY9JWY/W 8, 27 -- YnAzWgtrwT/ejRIwNd0VmJ3mV2/EfvQyjVNqVXPmYVG8GZ9WdTUR9GiaFPhydexgJOvj 8, 27 -- oBTw== 8, 27 -- MIME-Version: 1.0 8, 27 -- X-Received: by 10.13.213.212 with SMTP id x203mr14374080ywd.174.1434644104261; 8, 27 -- Thu, 18 Jun 2015 09:15:04 -0700 (PDT) 8, 27 -- Received: by 10.37.207.196 with HTTP; Thu, 18 Jun 2015 09:15:04 -0700 (PDT) 8, 27 -- Date: Thu, 18 Jun 2015 12:15:04 -0400 8, 27 -- Message-ID: {CAO806QS30gSMw3Ap=aHQjeRT0Zb00HOSsEmKDvHtCKdEiAKLEQ-at-mail.gmail.com} 8, 27 -- Subject: Discussion meeting on open source software for quantitative 8, 27 -- microanalysis at MM2015 8, 27 -- From: drix {drix00-at-gmail.com} 8, 27 -- To: Microscopy-at-microscopy.com 8, 27 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Thanks for the suggestion. Do you have a link to the description of the MSA Standards Committee? I check the MSA website and did not find anything.
Regards, Hendrix
On Thu, Jun 18, 2015 at 12:40 PM, Nestor Zaluzec - ANL {zaluzec-at-aaem.amc.anl.gov} wrote: } } Raynald etal } } An alternate venue for this might be via the MSA Standards Committee. } which meets during MM2015. That committee has members from MSA, MAS and } also AMAS. } } Nestor } Chair of the MSA Standards Committee } } } } } On Thursday, June 18, 2015, {drix00-at-gmail.com} wrote: } } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ---------------------------------------------------------------------------- } } } } Dear colleagues, } } } } We would like to invite you to a 2nd discussion meeting on open source } } software for quantitative microanalysis on Tuesday August 4th, 2015 } } after the second day of the MM2015 conference in Portland, Oregon. } } } } It goes without saying that the microanalysis community heavily } } depends on software. Quantification software, simulation programs, and } } physical quantities databases are essential tools for today's } } microanalysts. Broadly speaking, these software can be classified into } } four categories: commercial (sold by instrumentation's manufacturers } } or third-party companies), personal/research group internal or not } } openly distributed (XFILM, PENELOPE), free to download and use } } (WinCasino, CalcZAF) and open source (DTSA-II, pyPENELOPE). Without a } } doubt all types of software play an important role in the community, } } but we strongly believe that only open source software can foster } } innovation while offering the same level of validation and } } transparency as peer-reviewed scientific work. Although closed source } } software may be based on published equations, they remain black boxes } } as other equations are sometimes used in running codes. Alternate } } implementations, further optimization or special cases are not shared } } with the users. Most importantly, commercial, private and free } } software are a barrier to innovate as they force scientists, who would } } like to improve or develop new algorithms, to start from scratch. } } } } We would therefore propose to start a community-driven open source } } project to centralize physical quantity databases and algorithms used } } for quantification. The goal is to encourage collaborative work and } } provide the necessary building blocks for new projects in } } microanalysis. For more information, you can visit our website } } openmicroanalysis.org and discussion forum } } https://groups.google.com/forum/#!forum/openmicroanalysis, where the } } slides of the 1st meeting held at EMAS2015 are available. } } } } The meeting will take place on: } } Tuesday August 4th, 2015 at 4:00 to 5:00 PM in room B118 in Oregon } } Convention Center. } } } } You can gladly forward this invitation to other colleagues that may be } } interested in joining the discussion. } } } } Best regards, } } Hendrix, Raynald, Philippe and Silvia } } } } -- } } ----------------------------------------------------------- } } Hendrix Demers } } Research Associate } } --------------- } } Department of Mining and Materials Engineering } } Room 1250 Wong Building } } 3610 University Street } } Montreal QC H3A 0C5 } } Tel: 514-398-4755 ext.0225 } } Mobile: 514-927-6186 } } email: hendrix.demers-at-mail.mcgill.ca } } ----------------------------------------------------------- } } } } ==============================Original } } Headers============================== } } 8, 27 -- From drix00-at-gmail.com Thu Jun 18 11:15:06 2015 } } 8, 27 -- Received: from mail-yk0-f177.google.com (mail-yk0-f177.google.com } } [209.85.160.177]) } } 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id t5IGF6Kk001377 } } 8, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 11:15:06 } } -0500 } } 8, 27 -- Received: by ykfl8 with SMTP id l8so70222536ykf.1 } } 8, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 } } 09:15:04 -0700 (PDT) } } 8, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 8, 27 -- d=gmail.com; s=20120113; } } 8, 27 -- } } h=mime-version:date:message-id:subject:from:to:content-type; } } 8, 27 -- bh=957QdYSjl43iY9123CY92XeDPKa0oda44LO5G8Xu5tI=; } } 8, 27 -- } } b=b0jcojroipg40aNbk+zLSdE+h2uYx4ip12Qj002dJZWwKMev8wKIZPkSa4Qyu9058V } } 8, 27 -- } } vvQ1e+SEmywopt+fiW3otyOZbuwWgRjYQ5nCTMA8BckJ0Vtauyz9iEH2QDmj8+aven4I } } 8, 27 -- } } yYD2OeEB2r75COj1+cAArKnNoAjfqLYOJj28koT4Ni/0i3X8FRHHAgngK6GZotcI8y1w } } 8, 27 -- } } AaVOS/PZRaFjO5iZdFt21Yn6II8LybgYr2w6hyL49IxU1p3R/jSwlxpewcMldY9JWY/W } } 8, 27 -- } } YnAzWgtrwT/ejRIwNd0VmJ3mV2/EfvQyjVNqVXPmYVG8GZ9WdTUR9GiaFPhydexgJOvj } } 8, 27 -- oBTw== } } 8, 27 -- MIME-Version: 1.0 } } 8, 27 -- X-Received: by 10.13.213.212 with SMTP id } } x203mr14374080ywd.174.1434644104261; } } 8, 27 -- Thu, 18 Jun 2015 09:15:04 -0700 (PDT) } } 8, 27 -- Received: by 10.37.207.196 with HTTP; Thu, 18 Jun 2015 09:15:04 } } -0700 (PDT) } } 8, 27 -- Date: Thu, 18 Jun 2015 12:15:04 -0400 } } 8, 27 -- Message-ID: } } {CAO806QS30gSMw3Ap=aHQjeRT0Zb00HOSsEmKDvHtCKdEiAKLEQ-at-mail.gmail.com} } } 8, 27 -- Subject: Discussion meeting on open source software for } } quantitative } } 8, 27 -- microanalysis at MM2015 } } 8, 27 -- From: drix {drix00-at-gmail.com} } } 8, 27 -- To: Microscopy-at-microscopy.com } } 8, 27 -- Content-Type: text/plain; charset=UTF-8 } } ==============================End of - } } Headers============================== } } } } -- } ---===[|]===--- } =========================================== } } Dr. Nestor J. Zaluzec } Argonne National Laboratory } Electron Microscopy Center } NanoScience and Technology Division } Bldg 212 / A-143 } 9700 S. Cass Ave } Argonne, Illinois 60439 USA } } Tel: 530-NES-TORZ (530-637-8679) has Voice Mail } Lab: 630-252-7901 } } Email: Zaluzec-at-aaem.amc.anl.gov } } iChat: Zaluzec-at-AIM.com } Skype: Zaluzec } Polycom: 146.139.72.119 } TPM: http://tpm.amc.anl.gov } } } } Senior Scientist - Argonne National Laboratory } Fellow of the Microscopy Society of America } Senior Fellow the Computational Institute - University of Chicago } E.P. Wigner Fellow - Oak Ridge National Laboratory } Past President Microscopy Society of America } Adjunct Professor of Physics - Northern Illinois University & } the University of Illinois at Chicago } Visiting Professor of Microscopy - Manchester University } } =========================================== } TPMLab: http://tpm.amc.anl.gov } MMSite: http://www.amc.anl.gov } =========================================== } } The box said ... } "This program requires Win 95/98/NT or better..." } So I bought a Mac ! } } =========================================== } ---===[|]===--- }
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Email: microscopy.gmb-at-gmail.com Name: Gary Brown
Organization: Independent consultant
Title-Subject: [Filtered] Embedding resins
Message: I use one of two resins for embedding the catalyst samples and polymer samples we encounter in the advanced characterization lab of the major petrochemical corporation (from which I retired last year after 33 years in polymer microscopy).
Porous samples that are inorganic or high melting organic materials typically are vacuum infiltrated and embedded in LR White resin (hard grade without accelerator) in flat embedding molds of high density polyethylene (conventional BEEM capsules can also be used; silicone rubber molds should be avoided because the silicone can be swelled by the LR White), and cured at 70-80C overnight. This approach produces very hard, brittle blocks that cut exceptionally well. LR White with accelerator is not used in our lab to embed polymer samples because LR White can interact with the sample before polymer is complete. Important note: LR White containing no accelerator can be exposed to oxygen without curing problems.
Polymer samples (films, fabrics, fibers, etc.) are embedded at room temperature or 50C in EpoFix epoxy resin with accelerator (25:3 wt/wt resin:accelerator) according to the manufacturer's directions. (EpoFix is available from Electron Microscopy Sciences although Ted Pella and others may sell a similar resin.) It is important to note that the resin-accelerator mixture cures in about 90 minutes but phase separates upon standing during the initial 45 minutes or so of curing. To prevent this, the mixture must be manually stirred using a stirring rod, wooden splint, or equivalent for at least two minutes (timed) and continued stirring using a magnetic stir bar/stir plate until the resin-accelerator mixture is stable after 45 to 60 minutes curing; about 60 to 90 minutes, the viscosity of the curing resin gradually increases beyond a useable point. The resin-accelerator mixture may be cured at 50C for several hours or at ambient temperature overnight. Blocks cured at ambient temperature may be a little soft after curing overnight; an additional day or more of curing improves cutting performance. Heat curing is preferred if the sample can tolerate 50C temperature without annealing or melting. For example, high density polyethylenes and polypropylenes (melting temperatures ~130C and 155-160C, respectively) were cured at 50C but softer, lower density polyethylenes and elastomers containing low melting fractions that melt just above ambient temperatures and up to 60C are cured at ambient temperature.
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jun 18 18:10:13 2015 15, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5INADZr019357 15, 17 -- for {microscopy-at-microscopy.com} ; Thu, 18 Jun 2015 18:10:13 -0500 15, 17 -- Message-ID: {55834FD5.1080408-at-microscopy.com} 15, 17 -- Date: Thu, 18 Jun 2015 18:10:13 -0500 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:Embedding resins 15, 17 -- References: {201506181938.t5IJcM0i014181-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201506181938.t5IJcM0i014181-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201506181938.t5IJcM0i014181-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I got a lot of great suggestions for tools to simulate STEM-EDS probe broadening and Iâve been exploring different software packages, including ÂľSTEM and Dr. Probe. However, my challenge is that I am trying to simulate images from thin film interfacesâthat is an extended planar boundary between two single crystals.
While many of these programs explain in detail how to build simple single-crystal models for input, I am not sure how to construct a model for an interface to run the multislice calculations on. How does one define the supercell to consider an artificial boundary? Iâve searched through the manuals for these programs but they donât offer much guidance.
Iâd appreciate any thoughts or suggestions. Thanks again! ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] Advanced EELS and EFTEM Training School GmbH
Message: This is an intensive 4-day training school in Jßlich that incorporates lectures, computer laboratories and microscope practical classes to provide participants with comprehensive, hands-on training on advanced electron energy los spectroscopy (EELS) and energy-filtered TEM (EFTEM) topics. Practical sessions on the microscope will be given at the Ernst Ruska-Center for Microscopy in Jßlich using a Cs probe corrected FEI Titan equipped with a X-FEG and a fully loaded Enfinium ER and the Cs/Cc corrected Pico microscope equipped with monochromator, a X-FEG and GIF Quantum ERS system.
This course focuses on the advanced practice of EELS imaging and analysis in the (S)TEM microscope on advanced based EELS techniques. A good experience with electron microscopy and EELS is highly recommended. By the end of the course, participants can expect to get the best out of their EELS spectrometer and know how to best optimize the experimental conditions in order to capture the maximum amount of information out of the TEM samples using EELS.
For more details and online register, please go to: http://www.gatan.com/company/events/advanced-eels-and-eftem-training-school-gmbh
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==============================Original Headers============================== 17, 19 -- From microscopylistserver-noreply-at-microscopy.com Fri Jun 19 17:54:00 2015 17, 19 -- Received: from anlvpn001.nst.anl.gov (mac22.zaluzec.com [206.69.208.22]) 17, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5JMs07R027868 17, 19 -- for {microscopy-at-microscopy.com} ; Fri, 19 Jun 2015 17:54:00 -0500 17, 19 -- Message-ID: {55849D64.7010103-at-microscopy.com} 17, 19 -- Date: Fri, 19 Jun 2015 17:53:24 -0500 17, 19 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 19 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 19 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 17, 19 -- MIME-Version: 1.0 17, 19 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 19 -- Subject: =?UTF-8?B?dmlhV1dXOkFkdmFuY2VkIEVFTFMgYW5kIEVGVEVNIFRyYWluaW5nIFM=?= 17, 19 -- =?UTF-8?B?Y2hvb2wgR21iSCBhdCB0aGUgRXJuc3QgUnVza2EtQ2VudGVyIGZvciBNaWNyb3M=?= 17, 19 -- =?UTF-8?B?Y29weSBpbiBKw7xsaWNo?= 17, 19 -- References: {201506191720.t5JHK6ZC000805-at-ns.microscopy.com} 17, 19 -- In-Reply-To: {201506191720.t5JHK6ZC000805-at-ns.microscopy.com} 17, 19 -- X-Forwarded-Message-Id: {201506191720.t5JHK6ZC000805-at-ns.microscopy.com} 17, 19 -- Content-Type: text/plain; charset=UTF-8; format=flowed 17, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: erwrigh-at-emory.edu Name: Elizabeth Wright
Organization: Emory University
Title-Subject: [Filtered] Cryo-EM staff position at Emory University
Message: Dear Colleagues,
Cryo-EM Staff Position Available In The Robert P. Apkarian Integrated Electron Microscopy Core At Emory University
The Robert P. Apkarian Integrated Electron Microscopy Core of Emory University seeks an experienced cryo-electron microscopist to assist faculty, postdoctoral fellows, and students with cryo-electron microscopy projects.
FACILITY RESOURCES: The facility is equipped with a JEOL JEM-2200FS 200 kV FEG TEM with an in-column energy filter, phase plate system, Direct Electron DE-20 direct detection device, and Gatan 4k x 4k CCD camera; a JEOL JEM-1400 120 kV TEM with Gatan 2k x 2k CCD camera; a Hitachi H-7700 120 kV TEM, two ISI-TopCon DS FEG-SEMs; a Bal-Tec high pressure freezer; a Leica freeze substitution system; a Leica cryo-ultramicrotome; and other ancillary equipment.
QUALIFICATIONS: The ideal candidate will have a Ph.D. and postdoctoral experience in a relevant area of structural biology or biophysics and significant experience in the field of cryo-EM. Qualifications include experience with electron microscopy related laboratory research, experimental design of TEM experiments, cryo-EM for the direct-imaging of biological/soft matter. Familiarity with JEOL microscopes, cryo-EM data collection strategies, and single particle and electron tomography image processing are preferred. In addition, problem solving skills and a proven ability to learn and teach in a multidisciplinary environment, strong attention to detail and good organizational skills are advantageous.
Key responsibilities include, but are not limited to: 1) Negative stain EM and cryo-EM of biological and soft materials specimens; 2) Image analysis and three-dimensional reconstructions of EM data; 3) Operation of electron microscopes (TEM) and cryogenic instrumentation; 4) Image analysis and three-dimensional reconstructions of electron microscopy data; 5) Interpretation and presentation of two-dimensional averages and three-dimensional volume data and fitting of high-resolution data into electron density maps.
COMPENSATION: Emory University offers competitive compensation and benefits packages covering health insurance, retirement benefits, and savings programs.
Interested candidates should contact Dr. Elizabeth R. Wright (erwrigh-at-emory.edu) and include a CV, statement of accomplishments and electron microscopy skills, research interests, and contact information for three references.
The advertisement will remain open until the position is filled.
Regards,
Liz
-- Elizabeth R. Wright, PhD Assistant Professor Emory University erwrigh-at-emory.edu (404) 727-4665
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==============================Original Headers============================== 27, 17 -- From microscopylistserver-noreply-at-microscopy.com Sun Jun 21 11:43:09 2015 27, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 27, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5LGh90x019232 27, 17 -- for {microscopy-at-microscopy.com} ; Sun, 21 Jun 2015 11:43:09 -0500 27, 17 -- Message-ID: {5586E99D.9090105-at-microscopy.com} 27, 17 -- Date: Sun, 21 Jun 2015 11:43:09 -0500 27, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 27, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 27, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 27, 17 -- MIME-Version: 1.0 27, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 27, 17 -- Subject: viaWWW:Cryo-EM staff position at Emory University 27, 17 -- References: {201506210252.t5L2qhas021244-at-ns.microscopy.com} 27, 17 -- In-Reply-To: {201506210252.t5L2qhas021244-at-ns.microscopy.com} 27, 17 -- X-Forwarded-Message-Id: {201506210252.t5L2qhas021244-at-ns.microscopy.com} 27, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 27, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dsnixew-at-gmail.com Sun Jun 21 17:14:49 2015 Return-Path: {mike.sfsd4f564s6df45dsnixew-at-gmail.com} Received: from gmail.com ([180.150.230.114]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t5LMEkUe012164 for {microscopylistserverarchive5-at-microscopy.com} ; Sun, 21 Jun 2015 17:14:47 -0500 Message-ID: {C0DE874C.CF5E8CE6-at-gmail.com}
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
realname - Cristovao de Lemos Email - lemos_nh-at-ig.com.br EDUCATION - Graduate College QUESTION - To whom it may help,
I am contacting you in order to request some help to specify the more appropriate blade to my microscopy necessities. I am using a microtome HM 450 and I need a blade that allows me to prepare polyolefins samples (soft samples) to be analyzed at Scanning Eléctron Microscopy. My researches have identified a combination between a good blade + using a freezing spray. To be checked! So, the samples probably will be injection or compression molded and the surface could not be damaged, which normally happen when it is prepared in a room temperature. I found three options of blades as described below:
- Shandon Premium and Standard High-Profile Disposable Blades
- MB22 Premier Disposable Low-Profile Microtome Blade
- Edge-Rite Disposable Microtome Blades
Which one do you think is it more recommended? Otherwise, could you help me to specify the better one?
I am looking forward to hearing from you.
Thanks in advance and best regards,
==============================Original Headers============================== 12, 33 -- From oshel1pe-at-cmich.edu Mon Jun 22 07:19:59 2015 12, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 12, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5MCJxML022260 12, 33 -- for {microscopy-at-microscopy.com} ; Mon, 22 Jun 2015 07:19:59 -0500 12, 33 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 12, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t5MCJvg2003459 12, 33 -- for {microscopy-at-microscopy.com} ; Mon, 22 Jun 2015 08:19:58 -0400 12, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 12, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 12, 33 -- (TLS) id 14.3.195.1; Mon, 22 Jun 2015 08:19:57 -0400 12, 33 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 12, 33 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 12, 33 -- (TLS) id 14.3.195.1; Mon, 22 Jun 2015 08:19:57 -0400 12, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 12, 33 -- cas2.central.cmich.local (141.209.15.141) with Microsoft SMTP Server (TLS) id 12, 33 -- 14.3.195.1; Mon, 22 Jun 2015 08:19:56 -0400 12, 33 -- Message-ID: {5587FD6C.7070100-at-cmich.edu} 12, 33 -- Date: Mon, 22 Jun 2015 08:19:56 -0400 12, 33 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 12, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 12, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 33 -- MIME-Version: 1.0 12, 33 -- To: micro {microscopy-at-microscopy.com} 12, 33 -- Subject: Ask-a-Microscopist: Which blade for sectioining polyolefins? 12, 33 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 12, 33 -- Content-Transfer-Encoding: 8bit 12, 33 -- X-Originating-IP: [141.209.2.100] 12, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 12, 33 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 12, 33 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 12, 33 -- X-CanItPRO-Stream: default 12, 33 -- X-Canit-Stats-ID: 02OHojWFx - 3d19460f9d66 - 20150622 12, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
It is time to start thinking about the Fall Biological Conference at the Materials Research Laboratory here at UIUC!
November 4th, 2015 Attendee registration is $20 1 day of lectures and student competition, next day tours and open lab in my Bio service lab here at MRL, as well as some vendor demonstrations.
The theme this year is Bioengineering, with our keynote speaker, MRL Director - Dr. John Rogers. rogers.matse.illinois.edu/
Other speakers:
Dr. Rashid Bashir illinois.edu/ds/detail?search_type=all&search=&from_result_list=true&skinId=0&userId=rbashir&sub=
Dr Brian Cunningham illinois.edu/ds/detail?userId=bcunning&search_type=all&search=brian%20cunningham&from_result_list=true&skinId=0&sub=
Dr. Ryan Bailey www.chemistry.illinois.edu/faculty/Ryan_Bailey.html
Dr. Rohit Bhargava chemimage.illinois.edu
Dr. Elizabeth Driskell illinois.edu/ds/search?search_type=userid&search=edriskel&skinId=10776
We will also have room for 9 student competitors. Competition rules will be copied at the end of this email. Entree is via registration form for the conference, if competing, you will be refunded the $20.
Dear vendors! ___________________
Your possibilities:
Table: $200 / conference
Once you have a table, at registration you may want to consider the following on the registration form:
Pizza sponser $50 = name on poster, name in announcements to user, and name on intersession slide show. ( we found out that since is part of conference, you can do this)
Student competition Prize sponsor $99.99 - given to the student who wins the presentation & poster competition. Prize named after you, prize mentioned on M&M list server.
You can find the online registration at the link: my.mrl.illinois.edu/eventreg/
Attendees: _____________________ Your link for registration will soon be up at â mrl.illinois.edu/events/conferences-workshops
A way to upload your abstracts and chose to be a competitor will be in the registration form, we hope to have that up soon in the coming days.
Please join us for a fun day of lectures, science and getting to know each other, Please SAVE THE DATE!
Lou Ann
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230
Student Competition Nov 4th 2015 BioConference Prizes ⢠$99 for First Place, 2 categories: student and postdoc ⢠Name and photo announcement for MRL publications ⢠Announcement on the International Microscopy List serve ⢠Bonus: Competitors register for free.
Entry Requirements ⢠Must present a POSTER and a PRESENTATION
⢠Post Docs enter the post do category, Undergraduates and Graduate students enter the student category. ⢠Subject matter should be a biological- or a biological/materials science- project you have worked on, highlighting use of the core facilities of MRL, Beckman or IGB. ⢠Upload a 1/2 page abstract (2-3 images may be included) online with your registration by October 1st, 2015. ⢠Be available (a participant of the conference) during the Morning of Nov 4th, 2015 ⢠The presentation should include tutorial-like explanations of the principles of the instruments and techniques used. ⢠The presentation should use Keynote or PowerPoint or other presentation software compatible with the presentation system.
Limitations ⢠Entries accepted from meeting registrants only ⢠Participants are to be Graduate, Undergraduate Students or a post doc from an institution of higher learning ⢠Entries limited to 9 presentations and posters ⢠The presentation shall be 15 minutes long, followed by a 5-minute Q&A session ⢠One speaker per entry
Judging criteria (including but not limited to) ⢠Methods and instrumentation explained in tutorial fashion ⢠Knowledge of the instrument(s) and technique(s) shown ⢠Subject delivered to the audience in an understandable and ordered manor ⢠Delivery that demonstrates understanding rather than memorization ⢠Audio clarity of the presentation ⢠Effective visual aids ⢠Sufficient number of images ⢠Performance during Q&A ⢠Presentation within the allotted time ⢠Winners will be announced at the end of the day of competition
==============================Original Headers============================== 54, 43 -- From lamiller-at-illinois.edu Mon Jun 22 08:16:45 2015 54, 43 -- Received: from pps01.cites.illinois.edu (pps01.cites.illinois.edu [192.17.82.69]) 54, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5MDGiZM012959 54, 43 -- for {microscopy-at-microscopy.com} ; Mon, 22 Jun 2015 08:16:44 -0500 54, 43 -- Received: from chiht4.cites.illinois.edu (chiht4.cites.illinois.edu [64.22.177.76]) 54, 43 -- by pps01.cites.illinois.edu (8.14.5/8.14.5) with ESMTP id t5MDDbh3022072 54, 43 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 54, 43 -- for {microscopy-at-microscopy.com} ; Mon, 22 Jun 2015 08:16:39 -0500 54, 43 -- Received: from CHIMBX2.ad.uillinois.edu ([169.254.7.146]) by 54, 43 -- CHIHT4.ad.uillinois.edu ([64.22.177.76]) with mapi id 14.03.0224.002; Mon, 22 54, 43 -- Jun 2015 08:15:44 -0500 54, 43 -- From: "Miller, Lou A" {lamiller-at-illinois.edu} 54, 43 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 54, 43 -- Subject: U of Illinois, MRL Fall Biological Conference -- Vendor 54, 43 -- Registration is Open, Attendee Registration to soon open at same URL. 54, 43 -- Thread-Topic: U of Illinois, MRL Fall Biological Conference -- Vendor 54, 43 -- Registration is Open, Attendee Registration to soon open at same URL. 54, 43 -- Thread-Index: AQHQrO2LCyaHn7jWwUabTvesf6emxw== 54, 43 -- Date: Mon, 22 Jun 2015 13:15:44 +0000 54, 43 -- Message-ID: {D26F8F46-9AED-4267-A19D-561F6CF7E1A6-at-illinois.edu} 54, 43 -- Accept-Language: en-US 54, 43 -- Content-Language: en-US 54, 43 -- X-MS-Has-Attach: 54, 43 -- X-MS-TNEF-Correlator: 54, 43 -- x-originating-ip: [128.174.34.199] 54, 43 -- Content-Type: text/plain; charset="utf-8" 54, 43 -- Content-ID: {37A91E3E4B477849A0EA519125DC9C0B-at-mx.uillinois.edu} 54, 43 -- MIME-Version: 1.0 54, 43 -- X-Spam-Score: 0 54, 43 -- X-Spam-Details: rule=cautious_plus_nq_notspam policy=cautious_plus_nq score=0 54, 43 -- kscore.is_bulkscore=7.48158757168937e-11 kscore.compositescore=0 54, 43 -- circleOfTrustscore=0 compositescore=0.175289816837112 suspectscore=0 54, 43 -- recipient_domain_to_sender_totalscore=0 phishscore=0 bulkscore=0 54, 43 -- kscore.is_spamscore=0 rbsscore=0.175289816837112 54, 43 -- recipient_to_sender_totalscore=0 54, 43 -- recipient_domain_to_sender_domain_totalscore=0 spamscore=0 54, 43 -- recipient_to_sender_domain_totalscore=0 urlsuspectscore=0.175289816837112 54, 43 -- adultscore=0 classifier=spam adjust=0 reason=mlx scancount=1 54, 43 -- engine=7.0.1-1402240000 definitions=main-1506220222 54, 43 -- X-Spam-OrigSender: lamiller-at-illinois.edu 54, 43 -- X-Spam-Bar: 54, 43 -- Content-Transfer-Encoding: 8bit 54, 43 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t5MDGiZM012959 ==============================End of - Headers==============================
I am trying to do post embedment immuno gold labeling on brain tissueso far Ive had no signal, only uniform background.
The target is glycine. The resin being used is Epon8-12. The secondary antibody is gold conjugated with 15 nm particles. The tissue is fixed in 3% Paraformaldehyde and 1% Glutaraldehyde.
Ive tried etching (this disintegrates the tissue even with recommended 25% Na Ethoxide). Ive also tried deosmication with 1% Na Metaperiodate.
Does anyone have any recommendations for an antibody and/or protocol that has proven success?
Unfortunately, because this tissue is used for other purposes as well, I cannot change the fixation or embedment protocol. ( I already know LR white is optimal for immuno staining). Any recommendations must be for tissue that is already thin sectioned and collected on Ni Grids.
Thanks much, -- Anna Thiessen Smith Lab Manager Department of Auditory Neuroscience University Wisconsin Madison 1300 University Ave. Madison WI, 53703 608-262-0291
==============================Original Headers============================== 17, 38 -- From athiessen-at-wisc.edu Mon Jun 22 12:12:10 2015 17, 38 -- Received: from smtpauth3.wiscmail.wisc.edu (wmauth3.doit.wisc.edu [144.92.197.226]) 17, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5MHCA6C007738 17, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Jun 2015 12:12:10 -0500 17, 38 -- MIME-version: 1.0 17, 38 -- Content-disposition: inline 17, 38 -- Content-type: text/plain; charset=windows-1252 17, 38 -- Received: from avs-daemon.smtpauth3.wiscmail.wisc.edu by 17, 38 -- smtpauth3.wiscmail.wisc.edu 17, 38 -- (Oracle Communications Messaging Server 7.0.5.33.0 64bit (built Aug 27 2014)) 17, 38 -- id {0NQC00800UTT9A00-at-smtpauth3.wiscmail.wisc.edu} for 17, 38 -- Microscopy-at-microscopy.com; Mon, 22 Jun 2015 12:12:10 -0500 (CDT) 17, 38 -- X-Spam-PmxInfo: Server=avs-3, Version=6.1.1.2430161, 17, 38 -- Antispam-Engine: 2.7.2.2107409, Antispam-Data: 2015.6.22.165715, 17, 38 -- SenderIP=0.0.0.0 17, 38 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=0.0.0.0 17, 38 -- Received: from wiscmail.wisc.edu (unknown [144.92.8.48]) 17, 38 -- by smtpauth3.wiscmail.wisc.edu 17, 38 -- (Oracle Communications Messaging Server 7.0.5.33.0 64bit (built Aug 27 2014)) 17, 38 -- with ESMTPA id {0NQC005LBVS99R00-at-smtpauth3.wiscmail.wisc.edu} for 17, 38 -- Microscopy-at-microscopy.com; Mon, 22 Jun 2015 12:12:09 -0500 (CDT) 17, 38 -- Received: from [144.92.8.48] (Forwarded-For: 144.92.8.48) 17, 38 -- by wmweb8.doit.wisc.edu (mshttpd); Mon, 22 Jun 2015 12:12:09 -0500 17, 38 -- From: Anna Thiessen {athiessen-at-wisc.edu} 17, 38 -- To: Microscopy-at-microscopy.com 17, 38 -- Message-id: {7450b9171746da.5587fb99-at-wiscmail.wisc.edu} 17, 38 -- Date: Mon, 22 Jun 2015 12:12:09 -0500 17, 38 -- X-Mailer: Oracle Communications Messenger Express 7u4-25.01(7.0.4.25.0) 64bit 17, 38 -- (built Feb 29 2012) 17, 38 -- Content-language: en 17, 38 -- Subject: TEM: Post-Embedment Immunogold Labeling 17, 38 -- X-Accept-Language: en 17, 38 -- Priority: normal 17, 38 -- In-reply-to: {76b0f252175d14.558841e3-at-wiscmail.wisc.edu} 17, 38 -- References: {7790bb081746b9.558841a6-at-wiscmail.wisc.edu} 17, 38 -- {76b0f252175d14.558841e3-at-wiscmail.wisc.edu} 17, 38 -- Content-Transfer-Encoding: 8bit 17, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5MHCA6C007738 ==============================End of - Headers==============================
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Microscopy Society of NE Ohio (MSNO)
Title-Subject: [Filtered] MSNO Summer School 2015
Message: We are very glad to announce the MSNO Summer School 2015 (July 17th -at- Kent State University. Topic: "Microscopy of soft-matter materials"). The registration is open now and is free to MSNO members. I am attaching a flyer (with the tentative schedule) here. The latest updates can be found at http://www.msneo.org/2015-summer-school.html. The registration page is http://www.msneo.org/2015-summer-school-registration.html. If you have any questions, please feel free to let us know.
This is part of our new efforts to diversify our activities and to increase our MSNO membership value. For the first event, the instructors will be 3 of our MSNO board members, talking about what we are most familiar with. Hopefully this will become a new MSNO tradition and we'll have many more organizations to host future educational events on many topics.
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Does anyone out there has experience adjusting pH of uranyl acetate? If yes, what chemical should be used? The desired pH is 5+.
Thank you all for any advice.
Hong
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==============================Original Headers============================== 9, 36 -- From hyi-at-emory.edu Tue Jun 23 16:58:26 2015 9, 36 -- Received: from ws-mr3.cc.emory.edu (ws-mr3.cc.emory.edu [170.140.50.233]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5NLwPW5024551 9, 36 -- for {microscopy-at-microscopy.com} ; Tue, 23 Jun 2015 16:58:26 -0500 9, 36 -- Received: from e14edge2n.Emory.Edu (e14edge2n.cc.emory.edu [170.140.53.162]) 9, 36 -- by ws-mr3.cc.emory.edu (8.13.8/8.13.8) with ESMTP id t5NLwMwr023533 9, 36 -- for {microscopy-at-microscopy.com} ; Tue, 23 Jun 2015 17:58:22 -0400 9, 36 -- Received: from E14CH3W.Enterprise.emory.net (10.240.10.115) by 9, 36 -- e14edge2n.Emory.Edu (170.140.53.162) with Microsoft SMTP Server (TLS) id 9, 36 -- 14.3.235.1; Tue, 23 Jun 2015 17:58:22 -0400 9, 36 -- Received: from E14MBX14N.Enterprise.emory.net ([fe80::e464:5551:d968:fbcc]) by 9, 36 -- E14CH3W.Enterprise.emory.net ([::1]) with mapi id 14.03.0235.001; Tue, 23 Jun 9, 36 -- 2015 17:58:22 -0400 9, 36 -- From: "Yi, Hong" {hyi-at-emory.edu} 9, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 36 -- Subject: Adjusting pH of uranyl acetate 9, 36 -- Thread-Topic: Adjusting pH of uranyl acetate 9, 36 -- Thread-Index: AdCt/7hWUXTI2xvwS5iMuTdR70VFLQ== 9, 36 -- Date: Tue, 23 Jun 2015 21:58:21 +0000 9, 36 -- Message-ID: {8AACF890-BF93-4BFF-9E81-6F722A0FAAD4-at-emory.edu} 9, 36 -- Accept-Language: en-US 9, 36 -- Content-Language: en-US 9, 36 -- X-MS-Has-Attach: 9, 36 -- X-MS-TNEF-Correlator: 9, 36 -- Content-Type: text/plain; charset="us-ascii" 9, 36 -- Content-ID: {EC0A2DD40C70A34DBC66F6F906B38FB2-at-Enterprise.emory.net} 9, 36 -- MIME-Version: 1.0 9, 36 -- X-Emory-MailScanner-Information: Please contact the ISP for more information 9, 36 -- X-Emory-MailScanner-ID: t5NLwMwr023533 9, 36 -- X-Emory-MailScanner: Found to be clean 9, 36 -- X-Emory-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 9, 36 -- score=-1.428, required 8, autolearn=disabled, RP_MATCHES_RCVD -1.43) 9, 36 -- X-Emory-MailScanner-From: hyi-at-emory.edu 9, 36 -- X-Spam-Status: No 9, 36 -- Content-Transfer-Encoding: 8bit 9, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5NLwPW5024551 ==============================End of - Headers==============================
Uranyl acetate will change into something else (partly Uranyl hydroxide) if one attempts to raise the pH and doing so will likely alter its chemical behaviour. There is a paper by Tokuyasu from the late 70âs in which he describes the use of oxalic acid and some base to make âneutral uranyl acetateâ. But this is no longer UAc. It was used to generate a sort of positive membrane contrast in cryosections.
Sorry, I do not have the reference close, but it shouldnât be hard to find the paper on line.
Jan Aurion
} On 24/06/2015, at 09:59, hyi-at-emory.edu wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear. All: } } Does anyone out there has experience adjusting pH of uranyl acetate? If yes, what chemical should be used? The desired pH is 5+. } } Thank you all for any advice. } } Hong
==============================Original Headers============================== 6, 37 -- From leunissen-at-aurion.nl Tue Jun 23 17:11:02 2015 6, 37 -- Received: from nm9-vm9.access.bullet.mail.gq1.yahoo.com (nm9-vm9.access.bullet.mail.gq1.yahoo.com [216.39.63.247]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5NMB16p030055 6, 37 -- for {microscopy-at-microscopy.com} ; Tue, 23 Jun 2015 17:11:02 -0500 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1435097457; bh=fd8Wk7wxWtZ8YmAUn2/jMZa2dogXStPOFJ/YVKD3mGw=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=QEYCIP7gRTVkyavY4xjN0RwcMEfLKHdfTPdWGqS62nVpOBVVv3mVfgFh5k598VH5j9cWSc0J7m1bJc7cviulqL0huipX940STeiRjZ1hNhLYF+5D2owZh9Ivoq4oLoFVSA1RPw5qbl6FP3EFJUinNaDK3Y0SfUs5Byp9CJmBTDBxcvEQleyEMPlsW7U8mDwmOTFAnazQ5z9JVDRfeN7qYjfpPQ/p3pGGfOT7GfLvHXrHPeEQfvkIekfGtjy/c7OsVoeVHxYN77lMj+vJCNYqC7rZeLsWOxrjNbH9X3Lf/4PcLaX8zPy2Wu+SbOtxoAQ6q0qfEPX5L7a/ZSZR5LO9ZQ== 6, 37 -- Received: from [216.39.60.171] by nm9.access.bullet.mail.gq1.yahoo.com with NNFMP; 23 Jun 2015 22:10:57 -0000 6, 37 -- Received: from [124.108.96.24] by tm7.access.bullet.mail.gq1.yahoo.com with NNFMP; 23 Jun 2015 22:10:57 -0000 6, 37 -- Received: from [127.0.0.1] by omp1001.tnz.mail.aue.yahoo.com with NNFMP; 23 Jun 2015 22:10:57 -0000 6, 37 -- X-Yahoo-Newman-Id: 830.91503.bm-at-omp1001.tnz.mail.aue.yahoo.com 6, 37 -- Received: (qmail 34974 invoked by uid 1000); 23 Jun 2015 22:10:56 -0000 6, 37 -- Received: from 124.108.96.72 by rel103.mail.aue.yahoo.com with SMTP; Tue, 23 Jun 2015 22:10:56 +0000 6, 37 -- Received: (qmail 85679 invoked from network); 23 Jun 2015 22:10:56 -0000 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1435097456; bh=fd8Wk7wxWtZ8YmAUn2/jMZa2dogXStPOFJ/YVKD3mGw=; h=Content-Type:Mime-Version:Subject:From:In-Reply-To:Date:Content-Transfer-Encoding:Message-Id:References:To; b=G8wfZmNh37omwo+cyMJHMzIhQncg4RVo68DueBFU3clUZH0Rv3uh3BTwTnZeNG6SeXysuTEHZmrIa3CXrhQA/SswEGLH6H3dXH10IismgUuRFvnTjlHDPn/V6ORS/4YJuB5E7KF6GsqfVSwlSMRuZupIPAeLsh8rByXS6+8UC9o= 6, 37 -- X-Yahoo-Newman-Property: ymail-3 6, 37 -- X-YMail-OSG: JLdklusVM1mdb15AnOBNipesi8kYv2bqnrMqFO5TEGrp0FU 6, 37 -- JYsqkEq.aLfMZ9HfnULJs.HEhq2_HmjqNoN0s5343F9_.c40P2JOr01ca_Uh 6, 37 -- xIfsEOKZwRzv0QGHnaVk_52r0NNY79E0VwohRpDDBviOOxTQW.n52pQIP_2m 6, 37 -- JOyoNp9HqAG.YHAeWN..NRJ8EtkgGcQkOPQsJTbhk2N0wd0wODrdHeBLUk2E 6, 37 -- IZQ05X_o8KbjPcsR8CTUGwH3uoX10m13FX2UwvC1oESGbwJUTWpyzgmThkMu 6, 37 -- amr0DEtcFf5a.OArB4WS6Bdw6UDyMNop7QNB8Fpg8uW3acV2PouXuL0iuX1D 6, 37 -- u4HBWQZ1Vw3zyONqLINveLfLZjCn3wrGqO.ZYaK_SiOjlYQ6dymexEPIQ9lM 6, 37 -- fjqwrk9PAZDHDB.hB7BMc_0jeS7XAPqN.dXffCoEd7.sBXNDvbx7asux7m6f 6, 37 -- Jl4xJzOffWjllsloQCTLhEPxvMxfBO6eHJmgdNzp_iuGTtWs31W65hcJWIy_ 6, 37 -- zDUd212ccV6S5gOsOV0EFhOQJMo48E30HRWtxaHKsIgrtqDs- 6, 37 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 6, 37 -- Content-Type: text/plain; charset=utf-8 6, 37 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2098\)) 6, 37 -- Subject: Re: [Microscopy] Adjusting pH of uranyl acetate 6, 37 -- From: Jan Leunissen {leunissen-at-aurion.nl} 6, 37 -- In-Reply-To: {201506232159.t5NLxF6l024818-at-ns.microscopy.com} 6, 37 -- Date: Wed, 24 Jun 2015 10:10:54 +1200 6, 37 -- Message-Id: {6CB0DA04-0A3E-4E30-87C1-11A963C18816-at-aurion.nl} 6, 37 -- References: {201506232159.t5NLxF6l024818-at-ns.microscopy.com} 6, 37 -- To: microscopy-at-microscopy.com 6, 37 -- X-Mailer: Apple Mail (2.2098) 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5NMB16p030055 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dsrbcay-at-gmail.com Wed Jun 24 00:13:30 2015 Return-Path: {mike.sfsd4f564s6df45dsrbcay-at-gmail.com} Received: from gmail.com ([114.129.213.240]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t5O5DRcp008046 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 24 Jun 2015 00:13:29 -0500 Message-ID: {EA42E9BB.195D3EBF-at-gmail.com}
Dear colleagues,
We are happy to announce that the third edition of the Swiss Image-Based Screening Conference 2015 (SIBS 2015), 30.Sep -1.Oct in Basel, Switzerland is open for registration and abstract submission.
For this two-day international event, the scientific and organizing committee put a particular emphasis on the innovative aspects of cell-based assays design and development with screening applicability: new probes and tools, novel instrumental and methodological approaches will be covered by a panel of experienced researchers selected from academia and industry as invited speakers.
Please, visit the SIBS 2015 Conference website for the list of confirmed speakers, registration and for more information about the event: https://www.sibs2015.ethz.ch/
(It is advised to register early as Basel hotel prices can be high close to the event date and please note that the number of conference participants is limited).
We are looking forward to seeing you in Basel!
On behalf of the SIBS2015 scientific and organizing committee,
Thomas Horn and Gerardo Turcatti Co-Chairs of SIBS 2015
Dr. Thomas Horn, Head of the Single Cell Facility and the Laboratory Automation Facility Department of Biosystems Science and Engineering (D-BSSE) Swiss Federal Institute of Technology Zurich (ETH) Mattenstrasse 26, U1.46 CH 4058 Basel Switzerland
==============================Original Headers============================== 11, 27 -- From thomas.horn-at-bsse.ethz.ch Wed Jun 24 04:45:42 2015 11, 27 -- Received: from edge10.ethz.ch (edge10.ethz.ch [82.130.75.186]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5O9jfNT017217 11, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Jun 2015 04:45:42 -0500 11, 27 -- Received: from CAS12.d.ethz.ch (172.31.38.212) by edge10.ethz.ch 11, 27 -- (82.130.75.186) with Microsoft SMTP Server (TLS) id 14.3.195.1; Wed, 24 Jun 11, 27 -- 2015 11:45:37 +0200 11, 27 -- Received: from MBX13.d.ethz.ch ([fe80::c4aa:956e:5014:ce66]) by 11, 27 -- CAS12.d.ethz.ch ([fe80::7861:4ecb:7c42:cad4%10]) with mapi id 14.03.0195.001; 11, 27 -- Wed, 24 Jun 2015 11:45:40 +0200 11, 27 -- From: "Horn Thomas" {thomas.horn-at-bsse.ethz.ch} 11, 27 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 27 -- Subject: Swiss Image-Based Screening Conference 2015 in Basel, Switzerland 11, 27 -- Thread-Topic: Swiss Image-Based Screening Conference 2015 in Basel, 11, 27 -- Switzerland 11, 27 -- Thread-Index: AdCuYofc6eQCstUHSWSPjQc5patGiA== 11, 27 -- Date: Wed, 24 Jun 2015 09:45:40 +0000 11, 27 -- Message-ID: {02EA489736E52549B41463867B33154D42E59355-at-MBX13.d.ethz.ch} 11, 27 -- Accept-Language: de-DE, de-CH, en-US 11, 27 -- Content-Language: en-US 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- x-originating-ip: [129.132.97.141] 11, 27 -- Content-Type: text/plain; charset="us-ascii" 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5O9jfNT017217 ==============================End of - Headers==============================
In a cable carrying both low voltage and high voltage signals via multiple insulated wires, it is possible to check for degradation of insulation by measuring the insulation resistance. Since the resistance is very high, a high DC voltage is used, such as 1KV to 5KV. My question is: for a cable that is labeled "300V", is it safe to apply a test voltage higher than that? regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: 317-895-5630 Toll free: 800-374-8557 (in USA & Canada) Fax: 317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 2, 36 -- From donc-at-asmicro.com Wed Jun 24 09:02:42 2015 2, 36 -- Received: from resqmta-ch2-09v.sys.comcast.net (resqmta-ch2-09v.sys.comcast.net [69.252.207.41]) 2, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5OE2gAq019805 2, 36 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2015 09:02:42 -0500 2, 36 -- Received: from resomta-ch2-07v.sys.comcast.net ([69.252.207.103]) 2, 36 -- by resqmta-ch2-09v.sys.comcast.net with comcast 2, 36 -- id kE2W1q0052EPM3101E2hSc; Wed, 24 Jun 2015 14:02:41 +0000 2, 36 -- Received: from asm20 ([98.226.74.93]) 2, 36 -- by resomta-ch2-07v.sys.comcast.net with comcast 2, 36 -- id kE2h1q00420mEeS01E2hsx; Wed, 24 Jun 2015 14:02:41 +0000 2, 36 -- Message-ID: {62E6D56224304A6099CF93FA972A096B-at-asm20} 2, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 2, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com} 2, 36 -- Subject: Electrical cable testing 2, 36 -- Date: Wed, 24 Jun 2015 10:02:50 -0400 2, 36 -- MIME-Version: 1.0 2, 36 -- Content-Type: text/plain; 2, 36 -- format=flowed; 2, 36 -- charset="iso-8859-1"; 2, 36 -- reply-type=original 2, 36 -- Content-Transfer-Encoding: 7bit 2, 36 -- X-Priority: 3 2, 36 -- X-MSMail-Priority: Normal 2, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 2, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 2, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 2, 36 -- s=q20140121; t=1435154561; 2, 36 -- bh=yv4cToPl354mI4XCPMTBLnqqxPX/4PUo+vM80hnf1m8=; 2, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version: 2, 36 -- Content-Type; 2, 36 -- b=pP3TFxVFrza5Fq8w6pHhp9z/MNQxHaqgGQJAurhPr2zKG1EqNG2AFoOU0ZhRzMa6y 2, 36 -- Fdz57wVHtX3KPSh1btJjEPXldbfz9o0+mfCBDxfVWIgsKYOQk9/t6rn+4NP0Em4W29 2, 36 -- N+KhPgi4DCI/mC5m1jW9c61eASQX4dtF3tQMKLtGB8yEx5MKP2RpfKWwMAjuGy+1yw 2, 36 -- uxCVj91HXFjRFVTW72/bIHPX0ive0ZYIOj/iGrlTZq1wu1Pnfb+BUkaP6Tis2ppfQA 2, 36 -- rKgzR7WXs+02VtOntLIPqEngo+NdQiAs3MIsNwTYLuERpoJqeVQJO2tNLylbtGXka8 2, 36 -- rAObj1c6PGs0w== ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dan.buntman-at-beamservices.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: dan.buntman-at-beamservices.com Name: Dan Buntman
Organization: Beam Services, Inc.
Title-Subject: [Filtered] SMI3200 FIB system looking for a good home
Message: We have a Seiko SMI3200 FIB single beam system available for donation to any university or other non-profit organization. The system has only been used ~3,500hrs., is fully functional and currently operating in our facility with demonstrated resolution of {5nm. The recipient will be responsible for crating, shipping and installation costs. If you are interested please contact me at dan.buntman-at-beamservices.com.
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==============================Original Headers============================== 11, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Jun 24 18:03:49 2015 11, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5ON3mfZ000739 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2015 18:03:48 -0500 11, 17 -- Message-ID: {558B3754.8090000-at-microscopy.com} 11, 17 -- Date: Wed, 24 Jun 2015 18:03:48 -0500 11, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 11, 17 -- MIME-Version: 1.0 11, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 17 -- Subject: viaWWW:SMI3200 FIB system looking for a good home 11, 17 -- References: {201506241723.t5OHNnE8030468-at-ns.microscopy.com} 11, 17 -- In-Reply-To: {201506241723.t5OHNnE8030468-at-ns.microscopy.com} 11, 17 -- X-Forwarded-Message-Id: {201506241723.t5OHNnE8030468-at-ns.microscopy.com} 11, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From news3409384wur-at-gmail.com Thu Jun 25 04:51:35 2015 Return-Path: {news3409384wur-at-gmail.com} Received: from gmail.com ([103.27.109.44]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t5P9pWU1008073 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 25 Jun 2015 04:51:34 -0500 Message-ID: {B5102E85.0F4466AA-at-gmail.com}
Hi! I will be retiring on June 29th and would like to thank all of you in the electron and light microscopy world for 44 years of friendship and support. Since I started with a Siemens 101 at the VAMC in Iowa City in 1971 to the digital wonders of current electron and light microscopes at UCSF in San Francisco I have received training, guidance and encouragement from others in our community. Thank you.
If you wish to contact me after October please email me at Larry-at-SaintRubidium.com
Best wishes, Larry
-- Larry Ackerman, Specialist Electron Microscopy Lab Manager, Diabetes & Endocrinology Research Center Microscopy Core UCSF, Dept. of Anatomy, Rm S657 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-999-4758
==============================Original Headers============================== 7, 57 -- From btv1==618c24752f7==Larry.Ackerman-at-ucsf.edu Thu Jun 25 11:45:08 2015 7, 57 -- Received: from esa1.ucsf.iphmx.com (esa1.ucsf.iphmx.com [68.232.141.34]) 7, 57 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5PGj8YT020468 7, 57 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Jun 2015 11:45:08 -0500 7, 57 -- Received: from mcbmobwap002.ucsfmedicalcenter.org ([64.54.35.146]) 7, 57 -- by esa1.ucsf.iphmx.com with ESMTP/TLS/DHE-RSA-AES256-SHA; 25 Jun 2015 09:44:58 -0700 7, 57 -- X-AuditID: 40362392-f79936d000006367-f3-558c30095ecb 7, 57 -- Received: from bcuda4.ucsf.edu (otp005580ots.ucsfmedicalcenter.org [64.54.36.202]) 7, 57 -- by mcbmobwap002.ucsfmedicalcenter.org (Symantec Mail Security) with SMTP id AC.0B.25447.9003C855; Thu, 25 Jun 2015 09:44:57 -0700 (PDT) 7, 57 -- X-ASG-Debug-ID: 1435250673-04d82034ae6ad9c0002-1DjkGe 7, 57 -- Received: from EXHT01.net.ucsf.edu (mx.net.ucsf.edu [64.54.247.193]) by bcuda4.ucsf.edu with ESMTP id rstuA9sMQERCru1l (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-microscopy.com} ; Thu, 25 Jun 2015 09:44:33 -0700 (PDT) 7, 57 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu 7, 57 -- X-Barracuda-Apparent-Source-IP: 64.54.247.193 7, 57 -- Received: from [192.168.0.101] (128.218.141.134) by exht01.net.ucsf.edu 7, 57 -- (64.54.247.218) with Microsoft SMTP Server id 14.3.224.2; Thu, 25 Jun 2015 7, 57 -- 09:44:33 -0700 7, 57 -- Message-ID: {558C2FE9.2060406-at-ucsf.edu} 7, 57 -- Date: Thu, 25 Jun 2015 09:44:25 -0700 7, 57 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu} 7, 57 -- Reply-To: {larry.ackerman-at-ucsf.edu} 7, 57 -- Organization: Microscopy Core, UCSF 7, 57 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 7, 57 -- MIME-Version: 1.0 7, 57 -- To: {Microscopy-at-microscopy.com} 7, 57 -- Subject: Goodbye and Thanks 7, 57 -- Content-Type: text/plain; charset="utf-8"; format=flowed 7, 57 -- X-ASG-Orig-Subj: Goodbye and Thanks 7, 57 -- Content-Transfer-Encoding: 7bit 7, 57 -- X-Barracuda-Connect: mx.net.ucsf.edu[64.54.247.193] 7, 57 -- X-Barracuda-Start-Time: 1435250673 7, 57 -- X-Barracuda-Encrypted: AES128-SHA 7, 57 -- X-Barracuda-URL: https://bcuda4.ucsf.edu:443/cgi-mod/mark.cgi 7, 57 -- X-Virus-Scanned: by bsmtpd at ucsf.edu 7, 57 -- X-Barracuda-BRTS-Status: 1 7, 57 -- X-Barracuda-Spam-Score: 0.00 7, 57 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=4.5 KILL_LEVEL=5.0 tests= 7, 57 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.20179 7, 57 -- Rule breakdown below 7, 57 -- pts rule name description 7, 57 -- ---- ---------------------- -------------------------------------------------- 7, 57 -- X-CFilter-Loop: Reflected 7, 57 -- X-Brightmail-Tracker: H4sIAAAAAAAAA01Ta0gUYRTl2x13x21Hx/F120pzthLKx/bYEHpZZA8iKGj9IYSNOrqb+5Cd 7, 57 -- 3VLpx1L22t6WgiZoRrUJWlaogRmtUaY/jCLJ7KGUkpZlUlEg1nwza+2/wzn33Hvu/fhIJVOj 7, 57 -- 1pEWu4t32jkrq9IQ6SsXZiaHGk6aDA11iWlTnR51Otry7cQnxQ6UpVmdx1st+3hn6to9GnPT 7, 57 -- 27SiClXxVOVjwoO+E14USgK9Aho7fyhkHANP395QeZGGZOjXCH7X3A/xIlIUjNB/m5f5SQQT 7, 57 -- A/2iQS3yy8CTL1uTwd91EcklVxA8fvhChQWKToTG86ekWQS9ENonKqVZKjoFah89lzBD6+Fn 7, 57 -- zyUlxuEiLj/ok+qj6SyoeN9EyH0i4EnVBwLHiaLjofaM1D6SngNen0eNsZJOg6raXiTn0cPk 7, 57 -- 4AUk8/HQOl6jlPnl0Ds4ppYxC6dGPwZWT4DTXd1qeds1MNpvkelF8OrzoUC5Do5eHQpcbS58 7, 57 -- uT6okvEBONrTJV0N6D4EvQfLAj1nwwPfS+IsiqsO2qA6KGp1ULw6pGxArC03x+bI2c8VGQzL 7, 57 -- Uty5Qr6Nz7PkctZcHr94isNZcAtJb64vb0O/Dm3wI5pErJbybDxhYkK4fUKJzY9SSQUbTUHC 7, 57 -- SRMTluPIKzFzgjnb6bbyAhtFXUgRaeofneO2FvoRkEpROrIAS3lcSSnvdMgGP5pDEmwsZYwX 7, 57 -- +9MFnIsv5Pki3jmjGkmSBcqHe0Y4+QK+ON9idc3Iok+PFTpYkQcqyFA/Sia14tRpKZBQxNkE 7, 57 -- S0HAGEmlLRZZ7QyLTd0og6z7NdCoYAi7w87rYqkMqTmuMbvt/6bqYihvndfEhAcJ2D/zV8bQ 7, 57 -- EvFkkVQVdmvFn/R/LEONYnJWgMSuMTGlQkw5nu3FKV2cKzhl5R4vThlgcb3Ogy7vjdZkdt6b 7, 57 -- e2fTs10Rq74TzOGd7dn1ZRlxrS2arqyHzTcHVidd+jN6ev226g3Fmq1fS+/UNJ8L047s3kwr 7, 57 -- piosbRX9TfXRW3zzsvrKthuHjfuTjMeWG8oa9Ovmu651vJlwTzeTHabIqsnz1vAjIUN6x0gE 7, 57 -- 9y5xWGhtSdhYcny89S5LCGZu6WKlU+D+AkzIcRovBAAA ==============================End of - Headers==============================
Hello All, Our CM100 works again. The problem was in two capacitors C19 and C21. A man from local electronic workshop localized them and replaced them with new ones. Now, CRT on our CM100 works fine as before.
Thank you all again for your hints and valuable advices.
Oldrich
Here is the link to a summary: http://www2.biomed.cas.cz/~benada/CM100_trouble_crt.html
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Mon, 25 May 2015 03:41:44 -0500, benada-at-biomed.cas.cz wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello All, } We have a trouble with our old Philips CM100. The info CRT has gone. } About two weeks the image on it was very faint, but still resolvable. } Now the screen is completely dark. The problem is not in dimming } circuits because the dimming of panel's LEDs is working fine and can } be adjusted. I think that the problem is in CRT tube. Please does } anybody had to solve such problem? I find out that CRT tube } (M24-306WR/ED) is still available on the market. } } Thanking for any response in advance. } } Oldrich } } P.S. No response from service, up to now. } }
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Disclaimer: If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR.
==============================Original Headers============================== 11, 42 -- From benada-at-biomed.cas.cz Fri Jun 26 08:34:22 2015 11, 42 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5QDYLRt004586 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 26 Jun 2015 08:34:21 -0500 11, 42 -- X-ASG-Debug-ID: 1435325660-05011e634635b490001-4CH8be 11, 42 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id 35KLatG2Yip7YLgP (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Fri, 26 Jun 2015 15:34:20 +0200 (CEST) 11, 42 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 11, 42 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 11, 42 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 11, 42 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 11, 42 -- (No client certificate requested) 11, 42 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id D95452C80E9 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 26 Jun 2015 15:34:18 +0200 (CEST) 11, 42 -- Date: Fri, 26 Jun 2015 15:33:44 +0200 11, 42 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 11, 42 -- To: {microscopy-at-microscopy.com} 11, 42 -- Subject: Re: [Microscopy] Philips CM100 trouble 11, 42 -- Message-ID: {20150626153344.5f2afa9f-at-u117ob02} 11, 42 -- X-ASG-Orig-Subj: Re: [Microscopy] Philips CM100 trouble 11, 42 -- In-Reply-To: {201505250841.t4P8fi8J018867-at-ns.microscopy.com} 11, 42 -- References: {201505250841.t4P8fi8J018867-at-ns.microscopy.com} 11, 42 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 11, 42 -- =?UTF-8?B?xIxS?= 11, 42 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 11, 42 -- MIME-Version: 1.0 11, 42 -- Content-Type: text/plain; charset=US-ASCII 11, 42 -- Content-Transfer-Encoding: 7bit 11, 42 -- X-IoP-CAS-MailScanner-ID: D95452C80E9.E392B 11, 42 -- X-IoP-CAS-MailScanner: Processed 11, 42 -- X-Spam-Status: No 11, 42 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 11, 42 -- X-Barracuda-Start-Time: 1435325660 11, 42 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 11, 42 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 11, 42 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 11, 42 -- X-Barracuda-BRTS-Status: 1 11, 42 -- X-Barracuda-Spam-Score: 0.00 11, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 11, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.20204 11, 42 -- Rule breakdown below 11, 42 -- pts rule name description 11, 42 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both paul.cannard-at-huawei.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: paul.cannard-at-huawei.com Name: Paul Cannard
Organization: CIP Technologies/ Huawei
Title-Subject: [Filtered] Manual for ETPSEMRA Robinson BSE controller
Message: Does anybody have a PDF of the instruction manual of the ETPSEMRA controller for a Robinson BSE detector manufactured c.2000. We don't appear to have one (SEM was ex-demo and the manual probably just got overlooked) and I thought somebody out in the community might just have one. It is easy enough to operate but I'm not sure how to engage the quadrants separately.
Thanks, Paul
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==============================Original Headers============================== 13, 17 -- From microscopylistserver-noreply-at-microscopy.com Fri Jun 26 16:59:13 2015 13, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5QLxDHO024479 13, 17 -- for {microscopy-at-microscopy.com} ; Fri, 26 Jun 2015 16:59:13 -0500 13, 17 -- Message-ID: {558DCB30.4090503-at-microscopy.com} 13, 17 -- Date: Fri, 26 Jun 2015 16:59:12 -0500 13, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 13, 17 -- MIME-Version: 1.0 13, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 17 -- Subject: viaWWW:Manual for ETPSEMRA Robinson BSE controller 13, 17 -- References: {201506261526.t5QFQXTW032680-at-ns.microscopy.com} 13, 17 -- In-Reply-To: {201506261526.t5QFQXTW032680-at-ns.microscopy.com} 13, 17 -- X-Forwarded-Message-Id: {201506261526.t5QFQXTW032680-at-ns.microscopy.com} 13, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zhiqiang-at-pdx.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: zhiqiang-at-pdx.edu Name: Zhiqiang Chen
Organization: Portland State University
Title-Subject: [Filtered] Portland State University Workshop SURFACE ANALYSIS by XPS and AES
Message: Portland State University Workshop SURFACE ANALYSIS by XPS and AES
August 2nd 2015
Bldg. NASCC, Room 110, 710 SW Jackson, Portland OR 97201 ÂŹÂŹÂŹÂŹÂŹÂŹÂŹÂŹÂŹÂŹÂŹÂŹ The Center for Electron Microscopy and Nanofabrication at Portland State University and Physical Electronics cordially invite you to attend surface analysis Workshop including technical lectures and CEMN facility tour.
Technical topics will include: Â XPS/AES fundamentals. Â Basic Concept of the Scanning X-ray microprobe VersaProbe II system. Â XPS applications, AES and UPS applications. Â Spectrum data processing fundamentals including Peak ID, Quantification, Curve Fit, Depth Profiles (with LLS and Curve Fits), Chemical state mapping. Technical tutorial lectures will be given by Senior Staff Scientist, Dr. Sankar Raman at of Physical Electronics, followed by VersaProbe II system overview and demonstration sessions.
Please RSVP by July 27th to cemn.physics-at-gmail.com.
REGISTRATION: We have limited space for lectures and parking, please email us the registration form as soon as possible so that we can make reservation of parking space for you. Participants will be responsible for their own travel and lodging.
DEADLINES: Registration July 27, 2015 - late registrations will be NOT accepted. Registration info is available at http://www.pdx.edu/cemn/event/phi-psu-xps-workshop-surface-analysis-xps-and-aes?delta=0
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==============================Original Headers============================== 19, 18 -- From microscopylistserver-noreply-at-microscopy.com Sat Jun 27 06:49:25 2015 19, 18 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5RBnPZD032217 19, 18 -- for {microscopy-at-microscopy.com} ; Sat, 27 Jun 2015 06:49:25 -0500 19, 18 -- Message-ID: {558E8DC5.3030101-at-microscopy.com} 19, 18 -- Date: Sat, 27 Jun 2015 06:49:25 -0500 19, 18 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 18 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 18 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 19, 18 -- MIME-Version: 1.0 19, 18 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 18 -- Subject: viaWWW:Portland State University Workshop SURFACE ANALYSIS by XPS 19, 18 -- and AES on Aug 2, 2015 19, 18 -- References: {201506262335.t5QNZYwO032342-at-ns.microscopy.com} 19, 18 -- In-Reply-To: {201506262335.t5QNZYwO032342-at-ns.microscopy.com} 19, 18 -- X-Forwarded-Message-Id: {201506262335.t5QNZYwO032342-at-ns.microscopy.com} 19, 18 -- Content-Type: text/plain; charset=UTF-8; format=flowed 19, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From hanja07656515151tumaw-at-gmail.com Sun Jun 28 23:08:30 2015 Return-Path: {hanja07656515151tumaw-at-gmail.com} Received: from gmail.com ([1.215.227.86]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t5T48RqH011759 for {microscopylistserverarchive5-at-microscopy.com} ; Sun, 28 Jun 2015 23:08:29 -0500 Message-ID: {7E39567C.722C2ED8-at-gmail.com}
X-from: dlowry-at-asu.edu
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] TEM pre-embeddment labeling procedure
Message: We are looking for suggestions to resolve a problem with pre-embeddment labeling using the 1.4nm colloidal-gold conjugate and silver enhancement. The specimen is mouse brain and we are using vibratome sections of approx. 90-100 um thickness. We have attempted to follow a few published procedures with similar specimens but in preliminary trials we have encountered a large amount of background debris in the form of irregular-shaped, soft-edged dark spots of varying density approx 30-60 nm diameter. We suspect the main source of this is myelin sheath material due to its appearance and proximity. Here are particulars of the prep:
-perfusion fix with 3% PFA/0.2% GA in PBS -vibratome -re-immerse in perfusion mixture overnight 4C -wash with PBS -block sections with 10% goat-serum (NGS) in PBS with 0.3% Triton-X-100, 1 hr -wash with 1% NGS in PBS -1Â Ab in PBS with 1% NGS, 48 hrs 4C -wash with PBS/1% NGS -2Â Ab (Fab fragment) with 1.4nm gold in PBS/1% NGS, overnight 4C -wash with PBS only -1% GA fix in PBS -wash with deionized water (also tried using 0.02M citrate pH 7.0 here) -silver enhancement (various times, 30 sec to 4 min) -wash with deionized water -1% osmium and then continue with standard TEM prep, ultimately cutting 60 nm sections for observations
We have tried some variations such as bovine serum albumen instead of NGS during blocking and labeling, and tris-buffer instead of phosphate but the problem persists. We're planning to try thinner vibratome sections if possible. It seems a detergent is necessary to permeabilize the tissue but might it is also be solubilizing the myelin? Any suggestions are appreciated.
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==============================Original Headers============================== 17, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Jun 30 07:51:31 2015 17, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5UCpURo018499 17, 17 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jun 2015 07:51:31 -0500 17, 17 -- Message-ID: {559290D2.7060609-at-microscopy.com} 17, 17 -- Date: Tue, 30 Jun 2015 07:51:30 -0500 17, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 17, 17 -- MIME-Version: 1.0 17, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 17 -- Subject: viaWWW:TEM pre-embeddment labeling procedure 17, 17 -- References: {201506291727.t5THRIZA026576-at-ns.microscopy.com} 17, 17 -- In-Reply-To: {201506291727.t5THRIZA026576-at-ns.microscopy.com} 17, 17 -- X-Forwarded-Message-Id: {201506291727.t5THRIZA026576-at-ns.microscopy.com} 17, 17 -- Content-Type: text/plain; charset=UTF-8; format=flowed 17, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hi, all I have a user who wishes to polish fresh (non-dried) bone samples to around 20Âľm thickness and examine them in transmitted light and look at the surface of the sample with our VP-SEM. We have a Minimet. Does anyone out there have a polishing protocol to share? TIA for a full protocol or any helpful hints, Julian
-- Julian P.S. Smith III Director, Winthrop Microscopy Facility Dept. of Biology Winthrop University 349 Columbia Ave Rock Hill, SC 29733
803-323-2111 x6427 (vox) 803-323-3448 (fax) 803-524-2347 (cell) Research Website www.birdnest.org/smithj Personal Website www.rociada-east.net
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Is there artifact there if you skip silver enhancement? Or silver enhancement without a gold labeling step? You need to figure out if you are enhancing endogenous salts or if that is background binding of clumped gold antibodies. Have you tried gold enhancement (e.g., Nanoprobes)? I find gold enhancement works much better than silver enhancement. I would have thought the Triton X-100 would have permeabilized the sections.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] TEM pre-embeddment labeling procedure
Message: We are looking for suggestions to resolve a problem with pre-embeddment labeling using the 1.4nm colloidal-gold conjugate and silver enhancement. The specimen is mouse brain and we are using vibratome sections of approx. 90-100 um thickness. We have attempted to follow a few published procedures with similar specimens but in preliminary trials we have encountered a large amount of background debris in the form of irregular-shaped, soft-edged dark spots of varying density approx 30-60 nm diameter. We suspect the main source of this is myelin sheath material due to its appearance and proximity. Here are particulars of the prep:
-perfusion fix with 3% PFA/0.2% GA in PBS -vibratome -re-immerse in perfusion mixture overnight 4C -wash with PBS -block sections with 10% goat-serum (NGS) in PBS with 0.3% Triton-X-100, 1 hr -wash with 1% NGS in PBS -1Â' Ab in PBS with 1% NGS, 48 hrs 4C -wash with PBS/1% NGS -2Â' Ab (Fab fragment) with 1.4nm gold in PBS/1% NGS, overnight 4C -wash with PBS only -1% GA fix in PBS -wash with deionized water (also tried using 0.02M citrate pH 7.0 here) -silver enhancement (various times, 30 sec to 4 min) -wash with deionized water -1% osmium and then continue with standard TEM prep, ultimately cutting 60 nm sections for observations
We have tried some variations such as bovine serum albumen instead of NGS during blocking and labeling, and tris-buffer instead of phosphate but the problem persists. We're planning to try thinner vibratome sections if possible. It seems a detergent is necessary to permeabilize the tissue but might it is also be solubilizing the myelin? Any suggestions are appreciated.
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==============================Original Headers============================== 29, 28 -- From PhillipsT-at-missouri.edu Tue Jun 30 08:41:14 2015 29, 28 -- Received: from um-tip1-missouri-out.um.umsystem.edu (um-tip1-missouri-out.um.umsystem.edu [198.209.49.135]) 29, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5UDfDUo027182 29, 28 -- for {microscopy-at-microscopy.com} ; Tue, 30 Jun 2015 08:41:13 -0500 29, 28 -- X-IronPort-Anti-Spam-Filtered: true 29, 28 -- X-IronPort-Anti-Spam-Result: A2AcBQC9m5JV/9SeoM9ZA4MRVF8GvyEKhXgCgUY7EQEBAQEBAQGBCoQiAQEBBE43AgQBCBEDAQEBCwIKARAdGwEUCQkBBAoJCBUEiA4Nnz6hLgGFGQEBAQEBBQEBAQEBAQEbBItGgmsJAYEuEQEeAiESCwsBAYIaTR2BFAWHA4URN4c8AYRZhE+DaESDTpJxJmOBJ4FwbwEBgQMHFyOBAgEBAQ 29, 28 -- X-IPAS-Result: A2AcBQC9m5JV/9SeoM9ZA4MRVF8GvyEKhXgCgUY7EQEBAQEBAQGBCoQiAQEBBE43AgQBCBEDAQEBCwIKARAdGwEUCQkBBAoJCBUEiA4Nnz6hLgGFGQEBAQEBBQEBAQEBAQEbBItGgmsJAYEuEQEeAiESCwsBAYIaTR2BFAWHA4URN4c8AYRZhE+DaESDTpJxJmOBJ4FwbwEBgQMHFyOBAgEBAQ 29, 28 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 29, 28 -- by um-tip1-exch-relay.um.umsystem.edu with ESMTP; 30 Jun 2015 08:41:13 -0500 29, 28 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.190]) by 29, 28 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0235.001; 29, 28 -- Tue, 30 Jun 2015 08:41:12 -0500 29, 28 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 29, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 29, 28 -- Subject: Re: [Microscopy] viaWWW:TEM pre-embeddment labeling procedure 29, 28 -- Thread-Topic: [Microscopy] viaWWW:TEM pre-embeddment labeling procedure 29, 28 -- Thread-Index: AdCzOmDDswwmHuhxQ06E9d/UkAkvvQ== 29, 28 -- Date: Tue, 30 Jun 2015 13:41:12 +0000 29, 28 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DE71A6737-at-UM-MBX-T02.um.umsystem.edu} 29, 28 -- Accept-Language: en-US 29, 28 -- Content-Language: en-US 29, 28 -- X-MS-Has-Attach: 29, 28 -- X-MS-TNEF-Correlator: 29, 28 -- x-originating-ip: [128.206.81.115] 29, 28 -- Content-Type: text/plain; charset="iso-8859-1" 29, 28 -- MIME-Version: 1.0 29, 28 -- Content-Transfer-Encoding: 8bit 29, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t5UDfDUo027182 ==============================End of - Headers==============================
From yuantainr-at-yahoo.com.tw Tue Jun 30 16:52:04 2015 Return-Path: {yuantainr-at-yahoo.com.tw} Received: from mvb2000.localdomain ([59.46.162.244]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5ULq3xk009507; Tue, 30 Jun 2015 16:52:04 -0500 Received: from User (75-148-252-49-Houston.hfc.comcastbusiness.net [75.148.252.49]) by mvb2000.localdomain (Postfix) with ESMTP id 7CEB38A21B2A; Tue, 30 Jun 2015 19:15:15 +0800 (CST) Reply-To: {yuantainv-at-yahoo.com.tw}
X-from: Georgianne.Ciraolo-at-cchmc.org
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Organization: Cincinnati Children's Hospital Medical Center
Title-Subject: [Filtered] fixation of white adipose
Message: I have a researcher who is interested in doing EM on white adipose specifically the mitochondria found within. Does anyone have a specific protocol for adipose fixation?
Thank you in advance
Georgianne Ciraolo Sr. EM Tech Department of Pathology
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Unfortunately, I have little experience with these disposable blades. I do, however, have years of experience sectioning polyolefins using glass and diamond knives initially using a manual sledge microtome and a cryogenic ultramicrotome for the last 30 years.
You got my attention when you mentioned using a freezing spray to cool the polyolefin sample during microtomy. To cut polyolefins without severe deformation, one needs to cool the sample to or near its glass transition temperature (Tg). The Tg for polyproplene and polyethylene are 0 C to -10 C and approximately -100 C to -130 C, respectively. It is essential that the samples be cooled prior to and during cutting. This can be achieved by simultaneously cooling the sample in the sample chuck and the blade prior to and during cutting. I achieved this by carefully pouring a stream of liquid nitrogen (LN2) over the blade and the sample (while it is mounted in the chuck) for several minutes before cutting is started and continued without interruption during cutting. The Tg of polypropylene is sufficiently high that you might use a freezing spray. However, the amount of spray needed to adequately cool the sample prior to and during cutting may be very expensive. For this reason, I suggest LN2 for all cryomicrotomy applications.
Please feel free to contact me off-line as needed.
I wish you all the best,
Gary M Brown Polymer Microscopy Consultant microscopy.gmb-at-gmail.com
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realname - Cristovao de Lemos Email - lemos_nh-at-ig.com.br EDUCATION - Graduate College QUESTION - To whom it may help,
I am contacting you in order to request some help to specify the more appropriate blade to my microscopy necessities. I am using a microtome HM 450 and I need a blade that allows me to prepare polyolefins samples (soft samples) to be analyzed at Scanning ElĂŠctron Microscopy. My researches have identified a combination between a good blade + using a freezing spray. To be checked! So, the samples probably will be injection or compression molded and the surface could not be damaged, which normally happen when it is prepared in a room temperature. I found three options of blades as described below:
- Shandon⢠Premium and Standard High-Profile Disposable Blades
- MB22 Premier Disposable Low-Profile Microtome Blade
- Edge-Rite⢠Disposable Microtome Blades
Which one do you think is it more recommended? Otherwise, could you help me to specify the better one?
I am looking forward to hearing from you.
Thanks in advance and best regards,
==============================Original Headers============================== 12, 33 -- From oshel1pe-at-cmich.edu Mon Jun 22 07:19:59 2015 12, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 12, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t5MCJxML022260 12, 33 -- for {microscopy-at-microscopy.com} ; Mon, 22 Jun 2015 07:19:59 -0500 12, 33 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 12, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t5MCJvg2003459 12, 33 -- for {microscopy-at-microscopy.com} ; Mon, 22 Jun 2015 08:19:58 -0400 12, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 12, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with
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Email: plarson-at-ou.edu Name: Preston Larson
Organization: University of Oklahoma
Title-Subject: [Filtered] Looking for Zeiss Vacuum Flange
Message: Hi all,
We're looking for a couple of Zeiss SEM vacuum flanges, 7.5 cm diameter, 4 x 3 mm bolts. We are looking to modify these flanges for an application on an existing microscope.
Would anyone have access to any spare flanges perhaps off an unused or decommissioned microscope that they would be willing to part with?
If so, please contact me off list.
Thanks, Preston Larson plarson-at-ou.edu
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From mike.sfsd4f564s6df45dscuhay-at-gmail.com Thu Jul 2 10:46:35 2015 Return-Path: {mike.sfsd4f564s6df45dscuhay-at-gmail.com} Received: from gmail.com (133-136-99-118.savecom.net.tw [118.99.136.133] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t62FkWPw003476 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 2 Jul 2015 10:46:34 -0500 Message-ID: {9653FC97.C2D1358C-at-gmail.com}
Hi everyone, we acquired a gift of an electron energy-loss spectrometer and digital instrumentation for a Gatan DigiPEELS model 766 (ultimately to go on a TEM), but without any software; fortunately we had cables and are hoping to resurrect the system.
We are looking for a copy of some "driver" software, circa 1995 that is used to talk to a Gatan DMA card (our card is in a NuBus slot). We have version 1.7.5 of FilterControl and already know that version 1.7.1 was written for the PCI cards that followed the NuBus cards at about that time. In other words we have the wrong software and are seeking someone who can help us please.
In particular there is an application (called PEELS Control) and a Macintosh Extension; as described in the EL/P 3.0 Users Guide (page 2-5). If you have such a system, or used to have and still have the disk, or even can e-mail a disk image (zipped perhaps), the size is less than 800kB (an old double-density floppy disk) and we would very interested.
There are alternative paths to our salvation, one would be to get a suitable DMA card that fits a PCI bus - since we have a working version of FilterControl written for PCI. Suggestions, ideas and opinions could be shared here, or message offers of support to help resolve our impasse would be most welcome.
Thanks Rob Keyse
-- Robert Keyse, DPhil
Research Scientist Lehigh University
5, East Packer Avenue, Whitaker Laboratory Bethlehem, PA 18015-3194
From yuantainr-at-yahoo.com.tw Sun Jul 5 09:26:18 2015 Return-Path: {yuantainr-at-yahoo.com.tw} Received: from mail45.fssprus.ru (fssp45.ru [85.233.136.66] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t65EQHrA007021; Sun, 5 Jul 2015 09:26:18 -0500 Received: from mail45.fssprus.ru (localhost.localdomain [127.0.0.1]) by mail45.fssprus.ru (Postfix) with ESMTP id E8C9F8DE9737; Sun, 5 Jul 2015 14:45:10 +0300 (MSK) Received: from User (75-148-252-49-houston.hfc.comcastbusiness.net [75.148.252.49]) by mail45.fssprus.ru (Postfix) with ESMTP; Sun, 5 Jul 2015 14:45:10 +0300 (MSK) Reply-To: {yuantainv-at-yahoo.com.tw}
X-from: anaitbouda-at-cdta.dz
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Email: stones.fern-at-dol.gov Name: Fern Stones
Title-Subject: [Filtered] Elemental Hg analysis using SEM/EDS
Message: Anyone have experience looking for trace amount of elemental mercury in samples?
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From massnewsletter4654654bytua-at-gmail.com Wed Jul 8 11:52:18 2015 Return-Path: {massnewsletter4654654bytua-at-gmail.com} Received: from gmail.com ([128.134.244.125]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t68GqF40006123 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 8 Jul 2015 11:52:17 -0500 Message-ID: {3B99BBE4.F9EB37EE-at-gmail.com}
Dear listers,
We are currently looking for a post-doc here at ORNL, for someone who has experience in defect analysis in materials via (S)TEM, and related skills. The posting can be found at http://1.usa.gov/1JU927S , or at jobs.ornl.gov . I have pasted the description below, as well. Please pass this along to anyone who may be interested.
Thanks, Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Radiation Effects and Microstructural Analysis Group Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov http://web.ornl.gov/sci/psd/mst/remag/parish.shtml
Postdoctoral Research Associate - Plasma Material Interactions / NB50493913
End Posting Date 08/15/2015
Purpose Under general supervision, the incumbent will focus/research on electron microscopy and microstructural analysis of radiation effects and plasma-materials interactions in refractory metals. This position resides in the Radiation Effects and Microstructural Analysis Group in the Materials Science and Technology Division (MSTD), Physical Sciences Directorate (PSD) at Oak Ridge National Laboratory (ORNL).
The Radiation Effects and Microstructural Analysis Group (REMAG) at ORNL uses computer modeling and experimental characterization methods (microscopy, thermophysical and mechanical properties) to advance the science of radiation effects on materials. Most of the work is performed in the Low Activation Materials Development and Analysis (LAMDA) laboratory, which houses three FEI DualBeam focused ion beams, one JEOL J2100F field-emission 200 kV general-purpose TEM-STEM, and an FEI Talos F200X field-emission 200 kV analytical TEM-STEM. Other ORNL laboratories include ion beam lines, additional FIBs and TEMs, multiple aberration-corrected STEMs, and atom-probe tomography. This project will explore the fundamental behavior of plasma-materials interactions for fusion energy by exposing tungsten and related high-temperature materials to low-energy, high-flux plasmas and measuring the response of the material to the disposition of the injected plasma ions, with an eventual long-term goal of designing more robust plasma-facing materials for tokamak systems.
Major Duties/Responsibilities * Design and lead experiments in electron microscopy, particularly transmission electron microscopy (TEM), of defect structures formed in refractory metals under plasma and ion-irradiation exposure * Collaborate with plasma and ion-irradiation laboratories to produce plasma-exposed and ion-irradiated specimens for electron microscopy examination * Prepare TEM specimens through electropolishing and focused ion beam (FIB) techniques. Examine specimens using scanning electron microscopy (SEM) and electron backscatter diffraction (EBSD) * Travel domestically to collaborator facilities for plasma and / or ion beam exposures of specimens * Collaborate within the ORNL LAMDA lab to utilize other research teams' expertise (nanoindentation, positron annihilation spectroscopy, etc.) * Responsible for presenting and reporting research results and publishing scientific results in peer-reviewed journals in a timely manner * Ensure compliance with environment, safety, health and quality program requirements * Maintain strong commitment to the implementation and perpetuation of values and ethics Qualifications Required * A PhD in Materials Science and Engineering, or a closely related field, completed within the last 5 years * An excellent record of productive and creative research demonstrated by publications in peer-reviewed journals * Expertise, as measured through first-author publications and/or major conference presentations, in transmission electron microscopy (TEM) and related methods (e.g., STEM, analytical STEM) in defect analysis in structural materials * Expertise in TEM sample preparation methods, such as focused ion beam (FIB) or electropolishing * Excellent written and oral communication skills and the ability to communicate in English to an international scientific audience
QUALIFICATIONS DESIRED: Expertise in materials properties and the influence of defects and processing on materials, particularly metals, is strongly desired. Knowledge or expertise in radiation effects in materials or plasma-materials interactions is highly desirable. Experience or expertise in scanning electron microscopy (SEM), electron backscatter diffraction (EBSD), in situ TEM experiments, and related topics is highly desirable. Knowledge of MATLAB software and programming is helpful.
Appointments will initially be for 24 months with a possibility of an extension of up to 12 months. Initial appointments and extensions are subject to performance and availability of funding. Appointment will start beginning of October 2015.
Please provide a list of publications when applying for this position. Three letters of reference are required and can be uploaded to your profile or emailed directly to PSDrecruit-at-ornl.gov. Please include the title of the position in the subject line.
This position will remain open for a minimum of 5 days after which it will close when a qualified candidate is identified and/or hired.
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Notice: If the position requires a Security Clearance, reviews and tests for the absence of any illegal drug as defined in 10 CFR 707.4 will be conducted by the employer and a background investigation by the Federal government may be required to obtain an access authorization prior to employment and subsequent reinvestigations may be required. If the position is covered by the Counterintelligence Evaluation Program regulations at 10 CFR 709, a counterintelligence evaluation may include a counterintelligence-scope polygraph examination. ORNL is an equal opportunity employer. All qualified applicants, including individuals with disabilities and protected veterans, are encouraged to apply. UT-Battelle is an E-Verify Employer.
==============================Original Headers============================== 17, 37 -- From parishcm-at-ornl.gov Wed Jul 8 13:52:26 2015 17, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 17, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t68IqPMp012578 17, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jul 2015 13:52:26 -0500 17, 37 -- X-SG: RELAYLIST 17, 37 -- X-IronPort-AV: E=Sophos;i="5.15,433,1432612800"; 17, 37 -- d="scan'208";a="83686312" 17, 37 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 17, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 08 Jul 2015 14:52:25 -0400 17, 37 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151]) 17, 37 -- (using TLSv1 with cipher AES256-SHA (256/256 bits)) 17, 37 -- (No client certificate requested) 17, 37 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 29FDEE08A5 17, 37 -- for {Microscopy-at-Microscopy.Com} ; Wed, 8 Jul 2015 14:52:25 -0400 (EDT) 17, 37 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHOS31.ornl.gov 17, 37 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Wed, 8 Jul 17, 37 -- 2015 14:52:24 -0400 17, 37 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by 17, 37 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id 17, 37 -- 15.00.1104.000; Wed, 8 Jul 2015 14:52:24 -0400 17, 37 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 17, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 17, 37 -- Subject: Post-doc opening in plasma-materials interactions at ORNL 17, 37 -- Thread-Topic: Post-doc opening in plasma-materials interactions at ORNL 17, 37 -- Thread-Index: AdC5rzhlS+2YrldKTOCEjN1R//H3nQ== 17, 37 -- Date: Wed, 8 Jul 2015 18:52:24 +0000 17, 37 -- Message-ID: {0f2dcab6fe524253924a534f53a856d2-at-EXCHCS32.ornl.gov} 17, 37 -- Accept-Language: en-US 17, 37 -- Content-Language: en-US 17, 37 -- X-MS-Has-Attach: 17, 37 -- X-MS-TNEF-Correlator: 17, 37 -- x-ms-exchange-transport-fromentityheader: Hosted 17, 37 -- x-originating-ip: [128.219.12.132] 17, 37 -- Content-Type: text/plain; charset="us-ascii" 17, 37 -- MIME-Version: 1.0 17, 37 -- Content-Transfer-Encoding: 8bit 17, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t68IqPMp012578 ==============================End of - Headers==============================
From ferguson651651615asis-at-gmail.com Thu Jul 9 03:08:22 2015 Return-Path: {ferguson651651615asis-at-gmail.com} Received: from gmail.com ([203.235.143.27]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t6988JXj028776 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 9 Jul 2015 03:08:21 -0500 Message-ID: {251390C2.DE29450B-at-gmail.com}
To the Microscopy Community,
We are currently looking for an experienced person for the Chief Histology Technician position in the Department of Pathology at the College of Veterinary Medicine, University of Georgia. The posting can be found at www.ugajobsearch.com, Posting #20151272. A full description of the position may be found below. Please pass this along to anyone you feel may be interested.
Kind regards, Mary
***************** Mary Ard, BS, HT(ASCP), AAS EM Lab Coordinator Dept of Pathology College of Veterinary Medicine 501 D.W. Brooks Drive University of Georgia Athens, GA 30602 706-542-5537 maryard-at-uga.edu
CHIEF HISTOLOGY TECHNICIAN
The University of Georgia, College of Veterinary Medicine is dedicated to training future veterinarians, providing services to animal owners and veterinarians, and conducting investigations to improve the health of animals as well as people. The college benefits pets and their owners, food producing animals, and wildlife by offering the highest quality hospital and diagnostic laboratory services. We have the most technologically advanced facilities located on a university campus, and we are dedicated to safeguarding public health by studying emerging infectious diseases that affect both animal and human health.
Position Announcement: Chief Histology Technician Job Summary: This is a supervisory and technical position in the department management and preparation of tissue specimens for pathological examinations. The incumbent of this position is responsible for planning and supervising the work activities of other Histology Technicians. Work involves the hiring, mentoring, performance management, assigning work areas and duties, training new technicians, and instructing other technicians in histologic techniques when there are difficult work assignments. The Chief Histology Technician is responsible for getting work out on time and without error. Work is reviewed by a pathologist and instructions are received from same.
Qualifications: 1. High School Diploma and a minimum of 2 years college in an appropriate field, and at least 5 years of experience in a histology lab, or any equivalent combination of training and experience. Minimum of three (3) years supervisory experience. 2. Must have HT or HLT certification at time of appointment, with experience with immunohistochemistry. 3. Thorough knowledge of budgetary procedures to support the effective use of funds and support profitability. 4. Competent in ordering supplies and equipment ensuring cost effectiveness and waste minimization. 5. Ability to plan and organize work schedules for subordinates to ensure adequate and efficient staffing levels. Ability to supervise technical personnel including hiring, training, motivating, coaching or discipline, and performance assessments.
Compensation: Minimum annual salary is $43,694 or higher depending upon experience. In addition UGA employees have access to a choice of health plan options and vision, dental, short term and long term disability plans, generous leave accruals. Retirement plans include either Teachers Retirement System of Georgia or a choice of several 401(k) plans. Details may be found here: http://www.hr.uga.edu/benefits
To apply for this position please visit www.ugajobsearch.com then type in Posting Number 20151272. This will take you directly to Chief Histology Technician job opening. You must create an online application form, and please feel free to attach any supplemental documents you prefer to support your application.
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What is the glass transition temperature (Tg) of the non-sulfonated PS-co-DVB copolymer? I expect it would be appreciably higher than room temperature. Regardless, the crumbling of the sections on the dry knife indicates too low a cutting temperature.
The first pieces of information a microtomist needs to know about the polymer are (1) its composition (polarity, solvent susceptibility, etc.) and (2) the Tg of the polymer. Best microtomy results for virtually any polymer will be obtained by cryosectioning at or slightly below the Tg or at room temperature for high Tg polymers. Finally, my decades of experience in cryoultramicrotomy of polyolefin plastics/elastomers and block copolymers led me to believe that DRY sectioning is the best way to section these materials. I know that others have success in this area but I found that liquid cryoultramicrotomy just wasn't worth the trouble caused by wetting of the back of the knife and the sample, swelling of the sample, residue of DMSO on the sections, etc. Feel free to contact me off-line as needed.
I wish you all the best.
Gary Brown Consultant in Polymer Microscopy
"An expert is a person who has made all the mistakes which can be made in a very narrow field.â and "Prediction is very difficult, especially if it's about the future." Niels Bohr
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We are renovating our histology lab and are shopping around for some new or used equipment. Our main user is interested in a Leica 2155 microtome (he has used this instrument in the past) and I have been told that this is a good model in that it works well and is still serviceable. Does anyone else have anything to add about this instrument?
We are also in need for an embedding center. Our main user has used a Leica EG1150 and a Thermo Shandon Histocentre 2, but he is open to other models so long as they are still serviceable and reasonably priced.
Suggestions on a flotation work station/water bath are also welcome. Our main user has used a Triangle Biomed Sci FWS-120, but is open to other serviceable models.
Ideally, we'd purchase all 3 (used) items for ~$12,000. We do have a bigger budget if we have to spend more though.
I've looked around on ebay and various used instrument sites, but I thought I'd ask the experts for suggestions before I proceeded with any purchases.
Thank you in advance,
Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu
From yuantainr-at-yahoo.com.tw Fri Jul 10 12:27:51 2015 Return-Path: {yuantainr-at-yahoo.com.tw} Received: from doori11.dooricare.com ([211.218.144.13]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6AHRnDH013507; Fri, 10 Jul 2015 12:27:50 -0500 Received: from User ([41.138.168.164]) (authenticated bits=0) by doori11.dooricare.com (8.14.1/8.14.1) with ESMTP id t6AHEdJ9000593; Sat, 11 Jul 2015 02:14:45 +0900 Message-Id: {201507101714.t6AHEdJ9000593-at-doori11.dooricare.com} Reply-To: {yuantainv-at-yahoo.com.tw}
On Jul 7, 2015, at 12:16 AM, microscopy.listserver-at-gmail.com wrote:
} Name: Fern Stones } } Title-Subject: [Filtered] Elemental Hg analysis using SEM/EDS } } Message: Anyone have experience looking for trace amount of elemental } mercury in samples? } Dear Fern, Since no one else has posted on this, I examined some river sediment with EDS, and while I wasn't specifically looking for Hg, I did find many elements. I also had enough overvoltage to see even the k- lines. There are two problems that you face: The first is that EDS is not sensitive to amounts much smaller than 1%, and the second is that Hg is volatile, so the amount under the beam will be continually decreasing. If you have access to WDS, it will be easier to find small amounts of Hg before it goes away. Good luck. Yours, Bill
I also held back, as my acquaintance with mercury in the SEM is with a tooth filling, a mercury amalgam! Acquired when a student broke a tooth the "specimen" proved to be very interesting as an EDX investigation. Made up of mercury, silver, tin and copper, we pick up mercury if we jump to a new area but, dwelling too long on an area the mercury may not show .
For those who would like a nice EDX test specimen, ask a dentist, they often have a pot full of potential specimens.
Without any doubt specimens like this make great teaching material.
Regards
Steve
Steve Chapman FRMS Electron Microscopy Consultancy and Training www.emcourses.com Cell +44 (0)7711606967
-----Original Message---- - X-from: wtivol-at-sbcglobal.net [mailto:wtivol-at-sbcglobal.net] Sent: 11 July 2015 20:59 To: protrain-at-emcourses.com
wtivol-at-sbcglobal.net wrote: } } The first is that EDS } is not sensitive to amounts much smaller than 1%, and the second is } that Hg is volatile
SEM/EDS sensitivity can be much better. I've gotten better than 500 PPM detection limits, using an SDD at high currents (5 nA or so, but still less than a probe) for about 1 minute acquisition times (~15-20 million counts in the spectrum) with very careful sum-peak stripping.
Can't speak to volatility, but I suppose a cold stage plus area scanning might work if a yes/no answer is all that's needed and that meets your definition of "trace". The Hg La line is in a nice place WRT potential overlaps, with the exception of Ge K which is still more than 100 eV away.
Depending on the sample and the spatial resolution requirement, XRF might be a better tool for this if the sample is something like Bill's river sediment and you need one or two more orders of magnitude in sensitivity. No beam heating issues.
Rick Mott, PulseTor LLC
==============================Original Headers============================== 5, 18 -- From rmott-at-pulsetor.com Sun Jul 12 10:26:09 2015 5, 18 -- Received: from p3plsmtpa07-09.prod.phx3.secureserver.net (p3plsmtpa07-09.prod.phx3.secureserver.net [173.201.192.238]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6CFQ01w017178 5, 18 -- for {microscopy-at-microscopy.com} ; Sun, 12 Jul 2015 10:26:04 -0500 5, 18 -- Received: from [192.168.15.2] ([174.57.45.195]) 5, 18 -- by p3plsmtpa07-09.prod.phx3.secureserver.net with 5, 18 -- id rTRy1q0074CfpA601TRy3J; Sun, 12 Jul 2015 08:25:59 -0700 5, 18 -- Message-ID: {55A2871F.8040007-at-pulsetor.com} 5, 18 -- Date: Sun, 12 Jul 2015 11:26:23 -0400 5, 18 -- From: Rick Mott {rmott-at-pulsetor.com} 5, 18 -- User-Agent: Thunderbird 2.0.0.24 (Windows/20100228) 5, 18 -- MIME-Version: 1.0 5, 18 -- To: microscopy-at-microscopy.com 5, 18 -- Subject: Re: [Microscopy] Re: viaWWW:Elemental Hg analysis using SEM/EDS 5, 18 -- References: {201507112013.t6BKDFJD012013-at-ns.microscopy.com} 5, 18 -- In-Reply-To: {201507112013.t6BKDFJD012013-at-ns.microscopy.com} 5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
wtivol-at-sbcglobal.net wrote: } } The first is that EDS } is not sensitive to amounts much smaller than 1%, and the second is } that Hg is volatile
SEM/EDS sensitivity can be much better. I've gotten better than 500 PPM detection limits, using an SDD at high currents (5 nA or so, but still less than a probe) for about 1 minute acquisition times (~15-20 million counts in the spectrum) with very careful sum-peak stripping.
Can't speak to volatility, but I suppose a cold stage plus area scanning might work if a yes/no answer is all that's needed and that meets your definition of "trace". The Hg La line is in a nice place WRT potential overlaps, with the exception of Ge K which is still more than 100 eV away.
Depending on the sample and the spatial resolution requirement, XRF might be a better tool for this if the sample is something like Bill's river sediment and you need one or two more orders of magnitude in sensitivity. No beam heating issues.
Rick Mott, PulseTor LLC
==============================Original Headers============================== 5, 18 -- From rmott-at-pulsetor.com Sun Jul 12 11:20:16 2015 5, 18 -- Received: from p3plsmtpa07-09.prod.phx3.secureserver.net (p3plsmtpa07-09.prod.phx3.secureserver.net [173.201.192.238]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6CFQ01w017178 5, 18 -- for {microscopy-at-microscopy.com} ; Sun, 12 Jul 2015 10:26:04 -0500 5, 18 -- Received: from [192.168.15.2] ([174.57.45.195]) 5, 18 -- by p3plsmtpa07-09.prod.phx3.secureserver.net with 5, 18 -- id rTRy1q0074CfpA601TRy3J; Sun, 12 Jul 2015 08:25:59 -0700 5, 18 -- Message-ID: {55A2871F.8040007-at-pulsetor.com} 5, 18 -- Date: Sun, 12 Jul 2015 11:26:23 -0400 5, 18 -- From: Rick Mott {rmott-at-pulsetor.com} 5, 18 -- User-Agent: Thunderbird 2.0.0.24 (Windows/20100228) 5, 18 -- MIME-Version: 1.0 5, 18 -- To: microscopy-at-microscopy.com 5, 18 -- Subject: Re: [Microscopy] Re: viaWWW:Elemental Hg analysis using SEM/EDS 5, 18 -- References: {201507112013.t6BKDFJD012013-at-ns.microscopy.com} 5, 18 -- In-Reply-To: {201507112013.t6BKDFJD012013-at-ns.microscopy.com} 5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would suggest inductively coupled plasma- optical emission spectroscopy (ICP-OES) or inductively coupled plamsa- mass-spectrometry (ICP-MS) if you have access to them. ICP-MS is capable of ppq in the right conditions, and frequently in the ppt. Samples do not need to be liquid, they are digested or ashed, then digested, for introduction into the instruments. While SEM-EDS or XRF might be capable of detecting the element, the conversion from excitation volume, ZAF correction, and peak area to a ppm or similar number is non-trivial.
I hope this helps, and good luck with your research! -Allen J. Hall http://www.prairienanotech.com/
rmott-at-pulsetor.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } wtivol-at-sbcglobal.net wrote: } } } } } } The first is that EDS } } is not sensitive to amounts much smaller than 1%, and the second is } } that Hg is volatile
I have a Pentafet Link Oxford X-Ray Detector Module 5431 10 MMSQ, but the berilium window is broken. I need a similar/compatible detector to ressurrect the system. These days LN2 detectors are being abandoned and I have hopes that someone has one that may be offered or purchased at low enough price. We will of course take care of the additional cost of the transport. Can anybody help?
Best wishes and thanks in advance. Please contact-me to the e-mail below
Prof. A.P. Alves de Matos Head of Egas Moniz Electron Microscopy and Histopathology Centre, caparica, Portugal apamatos-at-gmail.com
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Email: oscar.rivera-at-uda.cl Name: Oscar Rivera
Organization: Universidad de Atacama
Title-Subject: [Filtered] Can molybdenum and sulfur be separated on EDS elemental maping?
Message: Difference between MoL and SK lines (2.307 and 2.293 keV) is smaller than energy resolution of many EDS detectors, so both peaks are generally overlapped.
This problem turns bigger in EDS elemental mapping, so I want to ask if there is a procedure or "philosopy" to confront this problem and obtain a representative and correct mapping from a sample.
Best Regards, ORRL
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jul 13 07:21:23 2015 14, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6DCLN59030126 14, 17 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jul 2015 07:21:23 -0500 14, 17 -- Message-ID: {55A3AD43.4050906-at-microscopy.com} 14, 17 -- Date: Mon, 13 Jul 2015 07:21:23 -0500 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:Can molybdenum and sulfur be separated on EDS elemental maping? 14, 17 -- References: {201507120321.t6C3LJML027684-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201507120321.t6C3LJML027684-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201507120321.t6C3LJML027684-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: es.smrln-at-gmail.com Name: Erin Summerlin
Organization: Materials Analysis Startup Lab
Title-Subject: [Filtered] Preparing Standards
Message: Does anyone have any tips or a good method for mounting standards in a standard block for SEM/Probe analysis? We got the Smithsonian microbeam standards recently and are trying to figure out the most effective way to create our own in-house standards block. Any help or advice would be much appreciated.
Erin
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We mount all of our standards in acrylics pucks with 35 pre-drilled holes. The advantage is that when the epoxy shrinks as it cures, the whole mount shrinks without introducing any cracks which catch abrasives, oil, etc.
On 7/13/2015 3:54 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: es.smrln-at-gmail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both es.smrln-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: es.smrln-at-gmail.com } Name: Erin Summerlin } } Organization: Materials Analysis Startup Lab } } Title-Subject: [Filtered] Preparing Standards } } Message: Does anyone have any tips or a good method for mounting standards in a standard block for } SEM/Probe analysis? We got the Smithsonian microbeam standards recently and are trying to figure } out the most effective way to create our own in-house standards block. Any help or advice would be } much appreciated. } } Erin } } Login Host: 97.77.39.58 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
-- John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1443 E. 13th Ave (541) 346-4655 (probe) Eugene, OR (541) 346-6854 (FAX) 97403-1241
Anyone have a protocol or information how to process LCM excised tissue collected on polymer cap for scanning and transmission EM?
We have FluoroNanogold tagged fungal hyphae. The target cells will be captured using a Leica LMD7000 and processed for EM.
My questions are;
1) Has this been done before? 2) Can EM processing be done inside the cap containing tissue? 3) If not, how does one remove the tissue from UV cured polymer cap?
Any help or advice would be much appreciated. Karen
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Dear Microscopy List Serve, you are welcome to come to our November 4th & 5th MRL biological conference.
A day of lectures and student competition, the next day will be tours, open lab in my bio-service lab, and vendor instrument demonstrations.
The user, the registration for the fall conference is only ----- $20 ------- Rules, registration, the schedule and conference poster are found at:
This year the emphasis is on Bioengineering, and includes speakers:
Dr. John Rogers, UIUC Materials Research Laboratory Electronics for the Human Body
Dr. Elizabeth Driskell, UIUC Veterinary Medicine Cellular and Organ Ultrastructure
Dr. Rashid Bashir, UIUC Bioengineering Building Biological Machines with Living Cells
Dr. Brian Cunningham, UIUC Bioengineering Quantitative Imaging of Live Cell Adhesion by Photonic Crystal Enhanced Microscopy
Dr. Ryan Bailey, UIUC Chemistry New Bioanalytical Tools
Dr. Rohit Bhargava, UIUC Bioengineering Chemical Imaging for Molecular and Material Anaysls in Cancer
** 9 student competitors
We are excited to have such a good line up, and a program that will be of interest to material sciences as well as biology.
We will once again have a student competition, students from other institutions are welcome. There is also a category for post docs to show a poster, give a 15 min. presentation, and the winner of each category will win $99. The topic should involve biological components, involve instrumentation that would be used in one of our core facilities (LM, confocal,TEM, SEM SIMMS etc See IGB, Beckman and MRL institutes under illinois.edu) and be in tutorial format.
Your names will be posted on the MRL website, and to the national microscopy list serve.
Students register, select yes on the competition, submit a 1/2 page abstract by October 15th. If your entry is selected, the registration fee will be refunded to you. ** Note a poster is expected.
Come join us for food, tours, lectures, vendors and a great educational experience!
email me with any questions
Lou Ann Miller Biological and Soft Materials at MRL
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230
Register for NCI's Frontiers in Light Microscopy Symposium
The Center for Cancer Research at the National Cancer Institute (NCI) invites you to a free, one-day national symposium titled "Frontiers in Light Microscopy". The event should be an exciting forum for discussion and debate on the current state of the field.
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Speakers:
- Joerg Bewersdorf, Yale School of Medicine, New Haven, CT - Peter Friedl, University of Texas MD Anderson Cancer Center, Houston, TX - Klaus Hahn, University of North Carolina School of Medicine, Chapel Hill, NC - Samie Jaffrey, Weill Cornell Medical College, New York, NY - Na Ji, HHMI/Janelia Research Campus, Ashburn, VA - Daniel Larson, NCI, Bethesda, MD - Wesley Legant, HHMI/Janelia Research Campus, Ashburn, VA - Jennifer Lippincott-Schwartz, National Institute of Child Health and Human Development, Bethesda, MD - Hari Shroff, National Institute of Biomedical Imaging and Bioengineering, Bethesda, MD - Roberto Weigert, NCI and National Institute of Dental and Craniofacial Research, Bethesda, MD
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Please mark November 17, 2015 on your calendar for this exciting event, and forward this e-mail invitation and the conference flyer to colleagues.
For conference-related questions, please contact conferenceplanning-at-mail.nih.gov.
-- Romina Cialdella Office of Communications and Public Liaison National Cancer Institute National Institutes of Health 9609 Medical Center Drive, 2E424 Rockville, MD 20850 Direct: 240-276-6659 romina.cialdella-at-nih.gov
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I have a JEOL JSM- 840A SEM, with Oxford ISIS EDS that has not worked for about 2 years. Before I send it to a scrapyard, I want to be sure it has no value to anyone else. About 2 years ago it developed a vacuum problem that resulted in serious oxidation of the diffusion pump oil. Even after cleaning the pumps and replacing the oil, I have been unable to achieve workable vacuum. Even before the vacuum problems the stigmators stopped working. The EDS was working until the SEM went down. I am certain that it is not economically feasible to resurrect the microscope, and I am offering it free to anyone that wants it, in whole or in part. Preference will be given to those wanting, and willing to cart away the largest portion. I am willing to ship small parts that can be safely packed and shipped by wrapping in a few layers of bubble wrap and putting in a medium sized box(at recipients expense, of course). Larger pieces must be picked up in Medford, MA , 5 miles north of Boston. I can provide assistance in loading. If you have any interest, please contact me off list.
____________________________ David J. Wilbur, Ph.D. Instrumentation Specialist Department of Chemistry Tufts University 62 Talbot Ave. Medford, MA 02155 voice: 617-627-2163 Fax: 617-627-3443 email: david.wilbur-at-tufts.edu
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Organization: Kansas State University
Title-Subject: [Filtered] How to process cells grown on Cover slip for TEM.
Message: Hi Listeners, I have one user, she wants to do TEM study of cultured Neurons on cover slip. She wants to preserve elongated nerve processes. We can not go with suspension culture and make its pellets. If any body could help me here. TIA.
Ravi.
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Title-Subject: [Filtered] Wear pattern of used sputter target.
Message: We have Denton Desk II Sputter coater. An Attached is the image of glowing sputter target. Is it need to replaced or it has more lifespan to go on.
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I have had success looking at drosophila testes that have been adhered to a coverslip and then procressed into resin for TEM.
I followed a method by J P Chang where you process the samples normally (I used a glass petri dish to contain the solutions with something on the bottom to hold up the coverslip and make it easier to pick up), infiltrate on a shaker plate and then embed on a mold that I made from silicon. To separate the coverslip from the resin I dipped the sample in liquid nitrogen briefly and didn't notice any changes in the tissue.
The paper is: Chang J.P. (1971) A new technique for separation of coverglass substrate from epoxy-embedded specimens for electron microscopy, 37, 370-377.
You can also process as normal, infiltrate in the glass petri dish on a shaker plate and then use Beem capsules to embed. You put some resin (don't fill it right up) in the Beem capsule and then carefully invert it onto the area of interest and carefully place in the oven. The capsules can be quite easy to tip over. They can be separated using liquid nitrogen also.
Good luck!
Jordan Taylor Microscopy Technician Manawatu Microscopy and Imaging Centre Massey University Private Bag 11-222 Palmerston North, 4442 Ph +64 6 356 9099 extn 84719
________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: 17 July 2015 12:16 To: Taylor, Jordan
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Organization: Kansas State University
Title-Subject: [Filtered] How to process cells grown on Cover slip for TEM.
Message: Hi Listeners, I have one user, she wants to do TEM study of cultured Neurons on cover slip. She wants to preserve elongated nerve processes. We can not go with suspension culture and make its pellets. If any body could help me here. TIA.
Ravi.
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==============================Original Headers============================== 13, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jul 16 18:43:21 2015 13, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6GNhLNr020641 13, 17 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jul 2015 18:43:21 -0500 13, 17 -- Message-ID: {55A84199.4080903-at-microscopy.com} 13, 17 -- Date: Thu, 16 Jul 2015 18:43:21 -0500 13, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 13, 17 -- MIME-Version: 1.0 13, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 17 -- Subject: viaWWW:How to process cells grown on Cover slip for TEM 13, 17 -- References: {201507162135.t6GLZZOD017953-at-ns.microscopy.com} 13, 17 -- In-Reply-To: {201507162135.t6GLZZOD017953-at-ns.microscopy.com} 13, 17 -- X-Forwarded-Message-Id: {201507162135.t6GLZZOD017953-at-ns.microscopy.com} 13, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 26, 32 -- From J.W.Taylor-at-massey.ac.nz Thu Jul 16 21:30:12 2015 26, 32 -- Received: from tur-issmail1.massey.ac.nz (tur-issmail1.massey.ac.nz [130.123.96.135]) 26, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6H2U96N030455 26, 32 -- for {Microscopy-at-Microscopy.com} ; Thu, 16 Jul 2015 21:30:10 -0500 26, 32 -- Received: from tur-mm5.massey.ac.nz (tur-mm5.massey.ac.nz [130.123.96.133]) 26, 32 -- by tur-issmail1.massey.ac.nz (Postfix) with ESMTP id DA0982F9 26, 32 -- for {Microscopy-at-Microscopy.com} ; Fri, 17 Jul 2015 14:30:05 +1200 (NZST) 26, 32 -- Received: from tur-exch-hub2.massey.ac.nz (Not Verified[10.100.189.3]) by tur-mm5.massey.ac.nz with Trustwave SEG 26, 32 -- id {B55a868ad0000} ; Fri, 17 Jul 2015 14:30:05 +1200 26, 32 -- Received: from TUR-EXCH-NODE2.massey.ac.nz ([fe80::6d90:b430:e061:20ed]) by 26, 32 -- tur-exch-hub2.massey.ac.nz ([fe80::b593:a83a:a6d1:9959%13]) with mapi id 26, 32 -- 14.03.0235.001; Fri, 17 Jul 2015 14:30:04 +1200 26, 32 -- From: "Taylor, Jordan" {J.W.Taylor-at-massey.ac.nz} 26, 32 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 26, 32 -- Subject: RE: [Microscopy] viaWWW:How to process cells grown on Cover slip 26, 32 -- for TEM 26, 32 -- Thread-Topic: [Microscopy] viaWWW:How to process cells grown on Cover slip 26, 32 -- for TEM 26, 32 -- Thread-Index: AQHQwCXHh8TUVIry3Uu1yBEdLk/0QZ3e7XWT 26, 32 -- Date: Fri, 17 Jul 2015 02:30:04 +0000 26, 32 -- Message-ID: {9B653BFF0A441142A2C9C552190A494B05071D80-at-tur-exch-node2.massey.ac.nz} 26, 32 -- References: {201507170016.t6H0G31p027031-at-ns.microscopy.com} 26, 32 -- In-Reply-To: {201507170016.t6H0G31p027031-at-ns.microscopy.com} 26, 32 -- Accept-Language: en-GB, en-NZ, en-US 26, 32 -- Content-Language: en-GB 26, 32 -- X-MS-Has-Attach: 26, 32 -- X-MS-TNEF-Correlator: 26, 32 -- x-originating-ip: [10.100.189.5] 26, 32 -- Content-Type: text/plain; charset="us-ascii" 26, 32 -- MIME-Version: 1.0 26, 32 -- Content-Transfer-Encoding: 8bit 26, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6H2U96N030455 ==============================End of - Headers==============================
This is the normal glow pattern of a Denton Desk II in use. We'd need to see a photo of the target itself when not it use. Top open, looking directly at the target to get a good image. If there are any holes in the target, especially in the area of the plasma annulus, the target is toasted and needs to be replaced.
Phil
} Email: ravi.thakkar369-at-gmail.com } Name: Ravi } } Title-Subject: [Filtered] Wear pattern of used sputter target. } } Message: We have Denton Desk II Sputter coater. An Attached is the image of glowing sputter target. } Is it need to replaced or it has more lifespan to go on. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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No problem, you can flat-embed the cultured cells on the coverslip. Process the coverslips + cells as you would if you had pellets. Probably can use just glutaraldehyde, no formalin, since these are spread cells. I'd also add 1% monomeric tannic acid to the glut and/or OsO4 to help preserve the membranes.
Do the resin infiltration with a nutator or the like, but try to limit the degrees of tilt (and slow speed), so you don't get the fluid everywhere. Go through to 100% resin as usual, then cut the bottom & cap off of a BEEM capsule, invert over the cells, Carefully! fill with 100% resin - don't get air bubbles! - and polymerize. After polymerization, drop the coverslip + BEEM capsule in LN2, and the capsule with pop off. Trim and section.
Phil
} This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com } Name: Ravi Thakkar } } Organization: Kansas State University } } Title-Subject: [Filtered] How to process cells grown on Cover slip for TEM. } } Message: Hi Listeners, } I have one user, she wants to do TEM study of cultured Neurons on cover slip. She wants to preserve } elongated nerve processes. We can not go with suspension culture and make its pellets. } If any body could help me here. } TIA. } } Ravi. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 33 -- From oshel1pe-at-cmich.edu Fri Jul 17 07:13:29 2015 5, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6HCDSgl032504 5, 33 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jul 2015 07:13:29 -0500 5, 33 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t6HCDR5k003823; 5, 33 -- Fri, 17 Jul 2015 08:13:27 -0400 5, 33 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 5, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 33 -- (TLS) id 14.3.248.2; Fri, 17 Jul 2015 08:13:27 -0400 5, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 33 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 5, 33 -- 14.3.248.2; Fri, 17 Jul 2015 08:13:26 -0400 5, 33 -- Message-ID: {55A8F167.4020403-at-cmich.edu} 5, 33 -- Date: Fri, 17 Jul 2015 08:13:27 -0400 5, 33 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 33 -- MIME-Version: 1.0 5, 33 -- To: micro {microscopy-at-microscopy.com} , {ravi.thakkar369-at-gmail.com} 5, 33 -- Subject: Re: [Microscopy] viaWWW:How to process cells grown on Cover slip 5, 33 -- for TEM 5, 33 -- References: {201507170012.t6H0CvlD021848-at-ns.microscopy.com} 5, 33 -- In-Reply-To: {201507170012.t6H0CvlD021848-at-ns.microscopy.com} 5, 33 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 33 -- Content-Transfer-Encoding: 7bit 5, 33 -- X-Originating-IP: [141.209.2.100] 5, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 33 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 33 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 5, 33 -- X-CanItPRO-Stream: default 5, 33 -- X-Canit-Stats-ID: 02ORodroX - 272c1074ae85 - 20150717 5, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
This is the normal glow pattern of a Denton Desk II in use. We'd need to see a photo of the target itself when not it use. Top open, looking directly at the target to get a good image. If there are any holes in the target, especially in the area of the plasma annulus, the target is toasted and needs to be replaced.
Phil
} Email: ravi.thakkar369-at-gmail.com } Name: Ravi } } Title-Subject: [Filtered] Wear pattern of used sputter target. } } Message: We have Denton Desk II Sputter coater. An Attached is the image of glowing sputter target. } Is it need to replaced or it has more lifespan to go on. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 32 -- From oshel1pe-at-cmich.edu Fri Jul 17 07:37:05 2015 4, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6HC09Vm025553 4, 32 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jul 2015 07:00:10 -0500 4, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 4, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t6HC06UY002482; 4, 32 -- Fri, 17 Jul 2015 08:00:06 -0400 4, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 4, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 4, 32 -- (TLS) id 14.3.248.2; Fri, 17 Jul 2015 08:00:06 -0400 4, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 4, 32 -- 14.3.248.2; Fri, 17 Jul 2015 08:00:06 -0400 4, 32 -- Message-ID: {55A8EE45.9030200-at-cmich.edu} 4, 32 -- Date: Fri, 17 Jul 2015 08:00:05 -0400 4, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 32 -- MIME-Version: 1.0 4, 32 -- To: micro {microscopy-at-microscopy.com} , {ravi.thakkar369-at-gmail.com} 4, 32 -- Subject: Re: [Microscopy] viaWWW:Wear pattern of used sputter target 4, 32 -- References: {201507170012.t6H0CvbJ021853-at-ns.microscopy.com} 4, 32 -- In-Reply-To: {201507170012.t6H0CvbJ021853-at-ns.microscopy.com} 4, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 32 -- Content-Transfer-Encoding: 7bit 4, 32 -- X-Originating-IP: [141.209.2.100] 4, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 4, 32 -- X-CanItPRO-Stream: default 4, 32 -- X-Canit-Stats-ID: 02ORo06iD - 540d4ccfe822 - 20150717 4, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nsa2-at-leicester.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] SEM of Trichomonas tenax
Message: Hello Everybody,
What is the current thinking regarding conventional SEM of protozoa? I have some Trichomonas tenax fixed in 2.5% glut that need processing for SEM (and TEM) and I was hoping to use my standard procedure through ethanol/HMDS. Will this be adequate, or will critical point drying yield superior results?
Also, what is the best method of transition? Does the sample need filtering, and I process the filter? Would they adhere to a subbed coverslip, or will they all just wash off? If using HMDS, can I process them in a centrifuge tube, and just sprinkle the dried sample onto a sticky tab? We have some microporous specimen capsules that I use for critical point drying, but fear the protozoa are too small, and there is not enough sample, to effectively retrieve them after processing.
If anyone does regular processing for TEM and has a reliable protocol for resin embedding protozoa, that would also be very useful!
Any suggestions would be welcome, I look forward to hearing how many of you are out the office enjoying the summer holidays.
Nat
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With a dubious target of 57mm, I, with fresh gloves, reemove it and lay it on a vertically oriented dissecting scope lamp. In a darkened room, one can easily detect pinhole defects in the anulus.
Hopefully helpful,
Fred
Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson-at-wcupa.edu ________________________________________ X-from: oshel1pe-at-cmich.edu {oshel1pe-at-cmich.edu} Sent: Friday, July 17, 2015 8:19 AM To: Monson, Frederick
Ravi,
This is the normal glow pattern of a Denton Desk II in use. We'd need to see a photo of the target itself when not it use. Top open, looking directly at the target to get a good image. If there are any holes in the target, especially in the area of the plasma annulus, the target is toasted and needs to be replaced.
Phil
} Email: ravi.thakkar369-at-gmail.com } Name: Ravi } } Title-Subject: [Filtered] Wear pattern of used sputter target. } } Message: We have Denton Desk II Sputter coater. An Attached is the image of glowing sputter target. } Is it need to replaced or it has more lifespan to go on. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 32 -- From oshel1pe-at-cmich.edu Fri Jul 17 07:00:10 2015 4, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6HC09Vm025553 4, 32 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jul 2015 07:00:10 -0500 4, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 4, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t6HC06UY002482; 4, 32 -- Fri, 17 Jul 2015 08:00:06 -0400 4, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 4, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 4, 32 -- (TLS) id 14.3.248.2; Fri, 17 Jul 2015 08:00:06 -0400 4, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 4, 32 -- 14.3.248.2; Fri, 17 Jul 2015 08:00:06 -0400 4, 32 -- Message-ID: {55A8EE45.9030200-at-cmich.edu} 4, 32 -- Date: Fri, 17 Jul 2015 08:00:05 -0400 4, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 32 -- MIME-Version: 1.0 4, 32 -- To: micro {microscopy-at-microscopy.com} , {ravi.thakkar369-at-gmail.com} 4, 32 -- Subject: Re: [Microscopy] viaWWW:Wear pattern of used sputter target 4, 32 -- References: {201507170012.t6H0CvbJ021853-at-ns.microscopy.com} 4, 32 -- In-Reply-To: {201507170012.t6H0CvbJ021853-at-ns.microscopy.com} 4, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 32 -- Content-Transfer-Encoding: 7bit 4, 32 -- X-Originating-IP: [141.209.2.100] 4, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 4, 32 -- X-CanItPRO-Stream: default 4, 32 -- X-Canit-Stats-ID: 02ORo06iD - 540d4ccfe822 - 20150717 4, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 12, 40 -- From FMonson-at-wcupa.edu Fri Jul 17 08:07:12 2015 12, 40 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.63.2]) 12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6HD7B6K032197 12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jul 2015 08:07:12 -0500 12, 40 -- X-ASG-Debug-ID: 1437138431-08911c358b4b91cd0001-4CH8be 12, 40 -- Received: from WCUXCHP10.PASSHE.LCL ([144.26.2.146]) by MX01.WCUPA.EDU with ESMTP id T1pA6FRKGLwrYvh4 (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NO); Fri, 17 Jul 2015 09:07:11 -0400 (EDT) 12, 40 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 12, 40 -- X-Barracuda-Apparent-Source-IP: 144.26.2.146 12, 40 -- X-ASG-Whitelist: Client 12, 40 -- Received: from WCUXCHP08.PASSHE.LCL (144.26.2.144) by WCUXCHP10.PASSHE.LCL 12, 40 -- (144.26.2.146) with Microsoft SMTP Server (TLS) id 15.0.847.32; Fri, 17 Jul 12, 40 -- 2015 09:07:10 -0400 12, 40 -- Received: from WCUXCHP08.PASSHE.LCL ([144.26.2.144]) by WCUXCHP08.PASSHE.LCL 12, 40 -- ([144.26.2.144]) with mapi id 15.00.0847.030; Fri, 17 Jul 2015 09:07:10 -0400 12, 40 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 12, 40 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 40 -- CC: "oshel1pe-at-cmich" {oshel1pe-at-cmich} 12, 40 -- Subject: RE: [Microscopy] Re: viaWWW:Wear pattern of used sputter target 12, 40 -- Thread-Topic: [Microscopy] Re: viaWWW:Wear pattern of used sputter target 12, 40 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: viaWWW:Wear pattern of used sputter target 12, 40 -- Thread-Index: AQHQwIrbGw+5IvEQX0KovVw+TX2MLJ3foIXG 12, 40 -- Date: Fri, 17 Jul 2015 13:07:10 +0000 12, 40 -- Message-ID: {92d45eef74bc44d18c87f6fe227d9e6a-at-WCUXCHP08.PASSHE.LCL} 12, 40 -- References: {201507171219.t6HCJRMC007470-at-ns.microscopy.com} 12, 40 -- In-Reply-To: {201507171219.t6HCJRMC007470-at-ns.microscopy.com} 12, 40 -- Accept-Language: en-US 12, 40 -- Content-Language: en-US 12, 40 -- X-MS-Has-Attach: 12, 40 -- X-MS-TNEF-Correlator: 12, 40 -- x-originating-ip: [144.26.2.61] 12, 40 -- Content-Type: text/plain; charset="us-ascii" 12, 40 -- MIME-Version: 1.0 12, 40 -- X-Barracuda-Connect: UNKNOWN[144.26.2.146] 12, 40 -- X-Barracuda-Start-Time: 1437138431 12, 40 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA 12, 40 -- X-Barracuda-URL: https://SPAMCONTROL.WCUPA.EDU:443/cgi-mod/mark.cgi 12, 40 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 12, 40 -- X-Barracuda-BRTS-Status: 1 12, 40 -- Content-Transfer-Encoding: 8bit 12, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6HD7B6K032197 ==============================End of - Headers==============================
No problem, you can flat-embed the cultured cells on the coverslip. Process the coverslips + cells as you would if you had pellets. Probably can use just glutaraldehyde, no formalin, since these are spread cells. I'd also add 1% monomeric tannic acid to the glut and/or OsO4 to help preserve the membranes.
Do the resin infiltration with a nutator or the like, but try to limit the degrees of tilt (and slow speed), so you don't get the fluid everywhere. Go through to 100% resin as usual, then cut the bottom & cap off of a BEEM capsule, invert over the cells, Carefully! fill with 100% resin - don't get air bubbles! - and polymerize. After polymerization, drop the coverslip + BEEM capsule in LN2, and the capsule with pop off. Trim and section.
Phil
} This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com } Name: Ravi Thakkar } } Organization: Kansas State University } } Title-Subject: [Filtered] How to process cells grown on Cover slip for TEM. } } Message: Hi Listeners, } I have one user, she wants to do TEM study of cultured Neurons on cover slip. She wants to preserve } elongated nerve processes. We can not go with suspension culture and make its pellets. } If any body could help me here. } TIA. } } Ravi. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 33 -- From oshel1pe-at-cmich.edu Fri Jul 17 08:13:34 2015 5, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6HCDSgl032504 5, 33 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jul 2015 07:13:29 -0500 5, 33 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t6HCDR5k003823; 5, 33 -- Fri, 17 Jul 2015 08:13:27 -0400 5, 33 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 5, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 33 -- (TLS) id 14.3.248.2; Fri, 17 Jul 2015 08:13:27 -0400 5, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 33 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 5, 33 -- 14.3.248.2; Fri, 17 Jul 2015 08:13:26 -0400 5, 33 -- Message-ID: {55A8F167.4020403-at-cmich.edu} 5, 33 -- Date: Fri, 17 Jul 2015 08:13:27 -0400 5, 33 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 33 -- MIME-Version: 1.0 5, 33 -- To: micro {microscopy-at-microscopy.com} , {ravi.thakkar369-at-gmail.com} 5, 33 -- Subject: Re: [Microscopy] viaWWW:How to process cells grown on Cover slip 5, 33 -- for TEM 5, 33 -- References: {201507170012.t6H0CvlD021848-at-ns.microscopy.com} 5, 33 -- In-Reply-To: {201507170012.t6H0CvlD021848-at-ns.microscopy.com} 5, 33 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 33 -- Content-Transfer-Encoding: 7bit 5, 33 -- X-Originating-IP: [141.209.2.100] 5, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 33 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 33 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 5, 33 -- X-CanItPRO-Stream: default 5, 33 -- X-Canit-Stats-ID: 02ORodroX - 272c1074ae85 - 20150717 5, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I filter protozoas onto a membrane filter (pore size determined by the critters, but anywhere from 0.45 ľm to 8 ľm). First, sputter coat the filters on both sides, so you filter the beasts onto a conductive surface. Then add fix (1 - 1.25% glut); obviously in your case, just filter the fixed critters. Go through the dehydration series in whatever filter apparatus you have. Then: If HMDS, go through a EtOH:HMDS seriese 2:1, 1:1, 1:2 then 3 X 100% HMDS. I find most protistans dry best at 60 deg C.
You can CPD the filters with critters, but some (sometimes most) of them may come off in the CPD and be lost. Othertimes, this works fine. Just be sure you know which side of the filter your critters are on - the simplest way is to look at the pattern on the filter support, as it will be embossed on the filter.
You might also try drying from tert-butyl alcohol. There's an article about this in the May 2014 issue of Microscopy Today.
I never use microporous capsules, I find they shed particles and clog CPD valves.
TEM: try processing with whatever your usual protocol is.
Protistans are so variable, you really have to experiment, or get a known good protocol from someone who does your particular organism - Trichomonas tenax, here.
From processing euglenoids, we find even congeneric taxa can vary greatly in their processing requirements, for both SEM & TEM.
Phil
} Email: nsa2-at-leicester.ac.uk } Name: Natalie Allcock } } Organization: University of Leicester } } Title-Subject: [Filtered] SEM of Trichomonas tenax } } Message: Hello Everybody, } } What is the current thinking regarding conventional SEM of protozoa? I have some Trichomonas tenax } fixed in 2.5% glut that need processing for SEM (and TEM) and I was hoping to use my standard } procedure through ethanol/HMDS. Will this be adequate, or will critical point drying yield superior } results? } } Also, what is the best method of transition? Does the sample need filtering, and I process the } filter? Would they adhere to a subbed coverslip, or will they all just wash off? If using HMDS, can } I process them in a centrifuge tube, and just sprinkle the dried sample onto a sticky tab? We have } some microporous specimen capsules that I use for critical point drying, but fear the protozoa are } too small, and there is not enough sample, to effectively retrieve them after processing. } } If anyone does regular processing for TEM and has a reliable protocol for resin embedding protozoa, } that would also be very useful! } } Any suggestions would be welcome, I look forward to hearing how many of you are out the office } enjoying the summer holidays. } } Nat -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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The other replies have been very good- light will help show a pin-hole in the target when viewed from the other side in a dark room. I wanted to add a few points that might be helpful for other deposition systems also. For thicker targets, a feeler gauge often used by woodworkers to duplicate a profile can be used to help determine the depth of the wear-groove. This gauge has a row of pins that slide when pushed and match the profile of the item being pushed against. This is helpful in harder to reach targets if the gauge can fit.
Often the mounting plate for the target is made of another material (stainless steel or copper) and this will show up in EDS or other analyses when the deposited film is analyzed if the wear track has broken through the target sufficiently.
Finally, a good metals reclaimer (and sometimes the target supplier) will be willing to pay for the remaining high purity metal target "scrap." This may help in purchasing the replacement target.
Wishing you success in your work and research, -Allen J. Hall http://www.prairienanotech.com/
OK, now that the baseball all-star game is over, and we did NOT get to see Max Scherzer pitch (hottest pitcher in major leagues-$210M contract), I am still burning with curiosity as to whether he may be related to Otto Scherzer. Wiki photographs reveal a startling physical resemblance. Any theories or actual knowledge out there?
One addition to J.W. Taylor's suggestion: I use Wheaton's coverlip jars for the solvent and resin steps (and sometimes the entire process). To keep track of which surface the cells are on, I have all of the coverslips "facing" the same way, & I've used a diamond scribe to mark one side of each jar. It is easier for me to do the resin steps with the coverslips vertical - I get less damage to the cs this way.
................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Organization: Kansas State University
Title-Subject: [Filtered] How to process cells grown on Cover slip for TEM.
Message: Hi Listeners, I have one user, she wants to do TEM study of cultured Neurons on cover slip. She wants to preserve elongated nerve processes. We can not go with suspension culture and make its pellets. If any body could help me here. TIA.
Ravi.
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Email: unocicrr-at-ornl.gov Name: Raymond Unocic
Organization: Center for Nanophase Materials Science Oak Ridge National Laboratory
Title-Subject: [Filtered] In situ S/TEM: Postdoctoral Research Position (ORNL)
Message: Dear Nestor
Is it possible to post a new postdoc position on the microscopy list server?
Direct Link: https://recruiting.ornl.gov/sap/bc/webdynpro/sap/hrrcf_a_posting_apply?PARAM=cG9zdF9pbnN0X2d1aWQ9MDA1MDU2QjQzOTc3MUVENThBRTBENTlCRTc1NkQ0MjUmY2FuZF90eXBlPUVYVA%3d%3d&sap-client=010&sap-language=EN#
The Microscopy Group in the Center for Nanophase Materials Sciences (CNMS) at Oak Ridge National Laboratory (ORNL) is seeking a highly motivated candidate to conduct microscopy research on energy materials using novel in situ S/TEM techniques. The selected candidate will work with scientists and users at the CNMS to develop in situ S/TEM methods and capabilities for operando studies of nanomaterials, which includes the development and use of in situ liquid, electrochemical, and gas-cell systems and developing data acquisition and analysis methodologies. This position will also be closely associated with a collaborative and multidisciplinary team of scientists at ORNLÂs Fluid Interface Reactions, Structures and Transport Energy Frontier Research Center (FIRST-EFRC). Major Duties/Responsibilities
 Conduct nanomaterials research using in situ TEM methods, which will include developing/optimizing methodologies for operando studies in liquid and gas environments
 Develop advanced data acquisition and data analysis methods towards streamlining in situ studies
 Collaborate with approved users at the CNMS on in situ microscopy experiments
 Responsible for presenting and reporting research results and publishing scientific results in peer-reviewed journals in a timely manner
 Ensure compliance with environment, safety, health and quality program requirements
 Maintain strong commitment to the implementation and perpetuation of values and ethics
Qualifications Required  A PhD in Materials Science & Engineering, Chemistry, Physics, or closely related field completed within the last 5 years  Strong background in aberration corrected STEM and EELS
 Hands-on practical expertise with in situ microscopy methods
 Background in big data analytics and simulations using MatLab and COMSOL modeling - National Instruments data acquisition hardware, LabVIEW programming, and FPGA programming experience considered a plus
 Excellent record of productive and creative research demonstrated by publications in peer-reviewed journals
 Excellent written and oral communication skills and the ability to communicate in English to an international scientific audience
 Ability to set priorities, multi-task, and adapt to ever changing needs with the proven ability to function well in a fast-paced environment
 Ability to work both independently and in a collaborative team environment Work Directions and Interfaces
Appointments will initially be for 24 months with a possibility of an extension of up to 12 months. Initial appointments and extensions are subject to performance and availability of funding.
Please provide a list of publications when applying for this position. Three letters of reference are required and can be uploaded to your profile or emailed directly to PSDrecruit-at-ornl.gov. Please include the title of the position in the subject line. This position will remain open for a minimum of 5 days after which it will close when a qualified candidate is identified and/or hired.
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Protists are notoriously variable things; different but closely-related strains can respond very differently to the same fixation protocol. So you might get good results the first time you try, or you might have to experiment. To answer your questions in order:
I usually use a CPD for SEM prep, and I've gotten good results with that. However, there are a few different types of CPD, some of which require careful operation. Thus, the results may depend both on the type of CPD and the operator. I've also used tert-butanol in a freeze-dryer with adequate results, although I've gotten better results in some cases with the CPD. I've never used HMDS but I've heard very good things about it.
For mounting, I've used both poly-L-lysine-coated coverslips (the round 12-mm types, which unfortunately tend to be expensive) and Isopore filters. Give the cells about an hour on the coverslip and they're stuck. Isopore filters don't require any treatment at all: I fix my cells in a Petri dish and just plunge them through the filter and that's enough. Of course you want to be careful not to apply back-pressure when removing the cartridge, handle anything with cells adhering to it gently, and keep it wet! A few seconds at most out of EtOH is fine, though.
As for TEM, I 'trap' my cells in 2% agarose after fixation. I do this by pipetting a shallow puddle of molten agarose onto a fresh slide (use a transfer pipette or a cut-off Gilson: narrow pipettes will clog!) and then pipetting 1-10 ľl of concentrated fixed cuture directly into the puddle, trying to suspend the cells directly in the middle of the water (agarose?) column. After the agarose sets, I cut out a ~1-mm cube around the cells, and do my dehydration and embedding with the cube. I'll typically get 4-6 cubes from a single fixation. One nice aspect of this protocol is that, for the resin-containing stages, the sample will float if it's not ready for the next change! I also have colleagues who instead spin down their cells and dehydrate and embed them 'free' in an Eppendorf tube, but my cultures are usually too sparse to deal with the amount of material one loses in that procedure.
Hope that helps -- please feel free to e-mail me if you need any clarification or further advice.
Cheers! Aaron
Aaron A. Heiss, Ph.D. Gerstner Scholar and Lerner-Gray Fellow Eunsoo Kim Laboratory Department of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York, NY 10024 USA
212-769-5838 aheiss-at-amnh.org ________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: July 17, 2015 9:11 AM To: Aaron Heiss
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Title-Subject: [Filtered] SEM of Trichomonas tenax
Message: Hello Everybody,
What is the current thinking regarding conventional SEM of protozoa? I have some Trichomonas tenax fixed in 2.5% glut that need processing for SEM (and TEM) and I was hoping to use my standard procedure through ethanol/HMDS. Will this be adequate, or will critical point drying yield superior results?
Also, what is the best method of transition? Does the sample need filtering, and I process the filter? Would they adhere to a subbed coverslip, or will they all just wash off? If using HMDS, can I process them in a centrifuge tube, and just sprinkle the dried sample onto a sticky tab? We have some microporous specimen capsules that I use for critical point drying, but fear the protozoa are too small, and there is not enough sample, to effectively retrieve them after processing.
If anyone does regular processing for TEM and has a reliable protocol for resin embedding protozoa, that would also be very useful!
Any suggestions would be welcome, I look forward to hearing how many of you are out the office enjoying the summer holidays.
Nat
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==============================Original Headers============================== 25, 42 -- From aheiss-at-amnh.org Sat Jul 18 18:36:33 2015 25, 42 -- Received: from mail-mx-002.amnh.org (mail-mx-002.amnh.org [216.73.244.167]) 25, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6INaWls029967 25, 42 -- for {Microscopy-at-microscopy.com} ; Sat, 18 Jul 2015 18:36:32 -0500 25, 42 -- X-ASG-Debug-ID: 1437262591-0426413b991048a0001-1DjkGe 25, 42 -- Received: from exchange.amnh.org ([172.16.8.237]) by mail-mx-002.amnh.org with ESMTP id BQzUzaBRChgCsTOt; Sat, 18 Jul 2015 19:36:31 -0400 (EDT) 25, 42 -- X-Barracuda-Envelope-From: aheiss-at-amnh.org 25, 42 -- Received: from MAIL-MBX-005.internal.amnh.org ([fe80::f8d9:d817:b12:fce8]) by 25, 42 -- MAIL-CASHT-002.internal.amnh.org ([::1]) with mapi id 14.03.0235.001; Sat, 18 25, 42 -- Jul 2015 19:36:31 -0400 25, 42 -- From: Aaron Heiss {aheiss-at-amnh.org} 25, 42 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , 25, 42 -- "nsa2-at-leicester.ac.uk" {nsa2-at-leicester.ac.uk} 25, 42 -- Subject: RE: [Microscopy] viaWWW:SEM of Trichomonas tenax 25, 42 -- Thread-Topic: [Microscopy] viaWWW:SEM of Trichomonas tenax 25, 42 -- X-ASG-Orig-Subj: RE: [Microscopy] viaWWW:SEM of Trichomonas tenax 25, 42 -- Thread-Index: AQHQwJIkEv8jAASI20u577BBYlXEv53h18j3 25, 42 -- Date: Sat, 18 Jul 2015 23:36:31 +0000 25, 42 -- Message-ID: {D024C7AADDC93D45A0B10B4C92A7BDBD55B65003-at-MAIL-MBX-005.internal.amnh.org} 25, 42 -- References: {201507171311.t6HDBnab012340-at-ns.microscopy.com} 25, 42 -- In-Reply-To: {201507171311.t6HDBnab012340-at-ns.microscopy.com} 25, 42 -- Accept-Language: en-CA, en-US 25, 42 -- Content-Language: en-CA 25, 42 -- X-MS-Has-Attach: 25, 42 -- X-MS-TNEF-Correlator: 25, 42 -- x-originating-ip: [172.16.8.249] 25, 42 -- Content-Type: text/plain; charset="iso-8859-1" 25, 42 -- MIME-Version: 1.0 25, 42 -- X-Barracuda-Connect: UNKNOWN[172.16.8.237] 25, 42 -- X-Barracuda-Start-Time: 1437262591 25, 42 -- X-Barracuda-URL: https://spam.amnh.org:443/cgi-mod/mark.cgi 25, 42 -- X-Virus-Scanned: by bsmtpd at amnh.org 25, 42 -- X-Barracuda-BRTS-Status: 1 25, 42 -- X-Barracuda-Spam-Score: 0.00 25, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=4.0 KILL_LEVEL=9.0 tests=BSF_SC0_MISMATCH_TO 25, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.20867 25, 42 -- Rule breakdown below 25, 42 -- pts rule name description 25, 42 -- ---- ---------------------- -------------------------------------------------- 25, 42 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header 25, 42 -- Content-Transfer-Encoding: 8bit 25, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6INaWls029967 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dsru-at-gmail.com Mon Jul 20 03:49:22 2015 Return-Path: {mike.sfsd4f564s6df45dsru-at-gmail.com} Received: from gmail.com (118-163-37-8.HINET-IP.hinet.net [118.163.37.8]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t6K8nJj2006522 for {microscopylistserverarchive5-at-microscopy.com} ; Mon, 20 Jul 2015 03:49:21 -0500 Message-ID: {A99BD6BE.B296CAEF-at-gmail.com}
The JEOL 840 I offered last week is spoken for. I underestimated the interest, and I regret I cannot meet all requests. Please, no more inquiries.
Dave Wilbur
____________________________ David J. Wilbur, Ph.D. Instrumentation Specialist Department of Chemistry Tufts University 62 Talbot Ave. Medford, MA 02155 voice: 617-627-2163 Fax: 617-627-3443 email: david.wilbur-at-tufts.edu
I have a JEOL JSM- 840A SEM, with Oxford ISIS EDS that has not worked for about 2 years. Before I send it to a scrapyard, I want to be sure it has no value to anyone else. About 2 years ago it developed a vacuum problem that resulted in serious oxidation of the diffusion pump oil. Even after cleaning the pumps and replacing the oil, I have been unable to achieve workable vacuum. Even before the vacuum problems the stigmators stopped working. The EDS was working until the SEM went down. I am certain that it is not economically feasible to resurrect the microscope, and I am offering it free to anyone that wants it, in whole or in part. Preference will be given to those wanting, and willing to cart away the largest portion. I am willing to ship small parts that can be safely packed and shipped by wrapping in a few layers of bubble wrap and putting in a medium sized box(at recipients expense, of course). Larger pieces must be picked up in Medford, MA , 5 miles north of Boston. I can provide assistance in loading. If you have any interest, please contact me off list.
____________________________ David J. Wilbur, Ph.D. Instrumentation Specialist Department of Chemistry Tufts University 62 Talbot Ave. Medford, MA 02155 voice: 617-627-2163 Fax: 617-627-3443 email: david.wilbur-at-tufts.edu
==============================Original Headers============================== 9, 31 -- From David.Wilbur-at-tufts.edu Mon Jul 20 09:24:38 2015 9, 31 -- Received: from smtpout-prod-03.uit.tufts.edu (smtpout-prod-03-relay.uit.tufts.edu [130.64.19.53]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6KEOcRF025887 9, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Jul 2015 09:24:38 -0500 9, 31 -- Received: from tabvmexcashub02.tufts.ad.tufts.edu ([10.246.136.82]:28737) 9, 31 -- by smtpout-prod-03.uit.tufts.edu with esmtps (TLSv1:AES128-SHA:128) 9, 31 -- (Exim 4.76) 9, 31 -- (envelope-from {David.Wilbur-at-tufts.edu} ) 9, 31 -- id 1ZHBzi-0000KI-Kr 9, 31 -- for Microscopy-at-microscopy.com; Mon, 20 Jul 2015 10:24:38 -0400 9, 31 -- Received: from SSVMEXDAG01MB01.tufts.ad.tufts.edu ([169.254.7.8]) by 9, 31 -- tabvmexcashub02.tufts.ad.tufts.edu ([10.246.136.82]) with mapi id 9, 31 -- 14.03.0210.002; Mon, 20 Jul 2015 10:24:37 -0400 9, 31 -- From: "Wilbur, David J." {David.Wilbur-at-tufts.edu} 9, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 31 -- Subject: [Microscopy] JEOL 840A SEM available (non-working)- No longer 9, 31 -- available 9, 31 -- Thread-Topic: [Microscopy] JEOL 840A SEM available (non-working)- No longer 9, 31 -- available 9, 31 -- Thread-Index: AdDC981umXdWxOFIQsOEJvE82LLr0Q== 9, 31 -- Date: Mon, 20 Jul 2015 14:24:38 +0000 9, 31 -- Message-ID: {128CA7701AE7DB40B6DC972AA91371419DD9D29C-at-SSVMEXDAG01MB01.tufts.ad.tufts.edu} 9, 31 -- Accept-Language: en-US 9, 31 -- Content-Language: en-US 9, 31 -- X-MS-Has-Attach: 9, 31 -- X-MS-TNEF-Correlator: 9, 31 -- x-originating-ip: [10.250.136.17] 9, 31 -- Content-Type: text/plain; charset="iso-8859-1" 9, 31 -- MIME-Version: 1.0 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6KEOcRF025887 ==============================End of - Headers==============================
Hello Fellow Microscopists, We have a position to fill here at the Johns Hopkins SOM Microscope Facility. Microscopy Facility Specialist Req. number 67234. All interested parties should apply with Human Resources:
We seek a microscopy specialist talented in fluorescence/confocal microscopy with emphasis on computer-based analysis. Prior experience with an image quantitation package (e.g. Volocity, ImageJ, Imaris, Metamorph, MatLab) is highly desirable. Strong analytical and organizational skills coupled with strong interpersonal and communication skills (both oral and written) are essential.
The primary duties and responsibilities of the job: The primary duties of the candidate will be to train users on microscope operation, and to train and provide image analysis and quantitation services. Basic knowledge of cell biology is critical for communicating with users. Helping users with troubleshooting and advising on specimen preparation and interpretation may be needed as part of user services on projects. Regular duties include maintaining microscope work areas and include collecting and maintaining a library of protocols, manuals and tutorials for users. With all duties, timely record-keeping (electronic & written) are required.
With 4 other staff members, the candidate will provide user support at the Johns Hopkins School of Medicine Microscope Facility. This facility provides light, fluorescence and electron microscopy services to 350+ users annually throughout Johns Hopkins using 17+ advanced microscopes and sophisticated preparatory equipment. Because of the diversity of equipment within the facility, the candidate must display resourceful independence and a willingness to learn. Ultimately, the candidate is expected to become an expert resource for image quantitation, as well as a resource for using, analyzing, and troubleshooting experiments with fluorescence/confocal microscopy spanning generic and highly specialized applications.
Qualifications
BS/BA in biological sciences, chemistry, or related field required. At least three years of relevant work experience is required. Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
NOTE: The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the School of Medicine HR Divisional Office at 410-955-2990. For TTY users, call via Maryland Relay or dial 711.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
Note: Job postings are updated daily and remain online until filled. See more in our FAQ.
EEO Is The Law Applicants to and employees of Johns Hopkins are protected under Federal law from discrimination on several bases. Learn more
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Stage error on Hitachi S-3500N SEM.
Message: Dear All,
We have Hitachi S-3500N SEM. We are facing one strange error "The moter is wrong or the hardware limit is working: please close the system". (We didn't go the extreme with stage movement in any of the axis) I checked the all cable connected to stage all are fit well.
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Email: sbarlow-at-mail.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] M&M 2015 room to share?
Message: Dear all M&M 2015 attendees in Portland
At the last minute my graduate assistant decided he would attend the upcoming meeting in Portland, his first M&M meeting.
Is there anyone with a room/housing that would consider allowing this grad student to share their space and the cost?
Please contact me offline at sbarlow-at-mail.sdsu.edu
Thanks for your consideration
Steve
-- Steven Barlow, Ph.D. Director, EM Facility/ SDSU Biology 5500 Campanile Drive MC-4614 San Diego, CA 92182-4614
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sbarlow-at-mail.sdsu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: sbarlow-at-mail.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] M&M 2015 room to share?
Message: Dear all M&M 2015 attendees in Portland
At the last minute my graduate assistant decided he would attend the upcoming meeting in Portland, his first M&M meeting.
Is there anyone with a room/housing that would consider allowing this grad student to share their space and the cost?
Please contact me offline at sbarlow-at-mail.sdsu.edu
Thanks for your consideration
Steve
-- Steven Barlow, Ph.D. Director, EM Facility/ SDSU Biology 5500 Campanile Drive MC-4614 San Diego, CA 92182-4614
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I've just updated my email client and it appears to have changed how forward mail address of WWW site submissions is delivered. You may see messages coming from "mail-at-microscopy.com" instead of "microscopylistserver-noreply". I will attempt to fix this, but it may take some time. Unfortunately and the next several weeks are very busy for me. So accept my apologies in advance. I will get it fixed but the time line is unknown at this point.
Cheers,
Nestor Your Friendly (and Perplexed) Neighborhood SysOp
==============================Original Headers============================== 7, 35 -- From anl.nestor.zaluzec-at-gmail.com Mon Jul 20 22:11:03 2015 7, 35 -- Received: from mail-ig0-f178.google.com (mail-ig0-f178.google.com [209.85.213.178]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6L3B3xK009147 7, 35 -- for {microscopy-at-microscopy.com} ; Mon, 20 Jul 2015 22:11:03 -0500 7, 35 -- Received: by igbpg9 with SMTP id pg9so96521983igb.0 7, 35 -- for {microscopy-at-microscopy.com} ; Mon, 20 Jul 2015 20:11:03 -0700 (PDT) 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 35 -- d=gmail.com; s=20120113; 7, 35 -- h=sender:from:content-type:content-transfer-encoding:subject:date 7, 35 -- :message-id:cc:to:mime-version; 7, 35 -- bh=gduiYLQT33xWFyu2DlDP+BE80Pdv7/O6rcgbjmoi4cY=; 7, 35 -- b=tyBJD5u6NxcVT7B6AzAp9m0BuuOjtiVf2gtOUQ1295CZvElv9CwrLx6TMa9nWGmHAi 7, 35 -- rwGFUKtUKethHZC13nbRdASclgP5E2F1mikYuIErC2GppnoPGQeqgqKhNyglSWVGzZ+b 7, 35 -- ORBsR080SHQ+/MC/DWbGUUgj+8BfMwtiWkwKaTC/RALzHFSkjgF1EZWFzU9OFUsF6ARd 7, 35 -- bAJ7Q/VmeHGvme7wjxdN9irdZUziZ9tr4lPMlGNDsBWNKXiEMOK2x1stfGc644+kl/MT 7, 35 -- GRcivPrTTPITJDZ8CabzmdY8SxN5V5kN1eTPG7j4wZfm2F5zxJwvCQTApK50KfkXgNQq 7, 35 -- lwkA== 7, 35 -- X-Received: by 10.107.129.215 with SMTP id l84mr24726036ioi.78.1437448263113; 7, 35 -- Mon, 20 Jul 2015 20:11:03 -0700 (PDT) 7, 35 -- Received: from [10.0.0.6] ([67.176.143.24]) 7, 35 -- by smtp.gmail.com with ESMTPSA id x4sm15000392iod.26.2015.07.20.20.11.02 7, 35 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 7, 35 -- Mon, 20 Jul 2015 20:11:02 -0700 (PDT) 7, 35 -- Sender: "ANL.Nestor Zaluzec" {anl.nestor.zaluzec-at-gmail.com} 7, 35 -- From: "Nestor J. Zaluzec - ANL/GAccnt" {zaluzec-at-aaem.amc.anl.gov} 7, 35 -- Content-Type: text/plain; charset=us-ascii 7, 35 -- Subject: Administrivia: Listserver Address oddities 7, 35 -- Date: Mon, 20 Jul 2015 22:10:57 -0500 7, 35 -- Message-Id: {7B7B7A1A-944F-4A02-B957-4F0FD88D1AD8-at-aaem.amc.anl.gov} 7, 35 -- Cc: "Zaluzec-ANL Nestor J." {zaluzec-at-aaem.amc.anl.gov} 7, 35 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 35 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.6\)) 7, 35 -- X-Mailer: Apple Mail (2.1878.6) 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6L3B3xK009147 ==============================End of - Headers==============================
I spotted this kickstarter proposal developing very small but rather bright lightsources and wondered how it compared with others currently available. Seems a convenient way of illuminating samples on a dissecting microscope, though it's really designed for photographers. The peak wavelength lights (red, green, blue, amber) look interesting too.
I've been very, very pleased with a pair of IKEA Jansjo lamps (60cm version with base) for dissection microscope illumination. Homogeneous beam, high output, cheaper than anything else (Ł10 GBP).
Ben ________________________________________ X-from: Rosemary.White-at-csiro.au [Rosemary.White-at-csiro.au] Sent: 21 July 2015 07:42 To: Ben Micklem
Dear all,
I spotted this kickstarter proposal developing very small but rather bright lightsources and wondered how it compared with others currently available. Seems a convenient way of illuminating samples on a dissecting microscope, though it's really designed for photographers. The peak wavelength lights (red, green, blue, amber) look interesting too.
==============================Original Headers============================== 10, 31 -- From ben.micklem-at-pharm.ox.ac.uk Tue Jul 21 02:40:00 2015 10, 31 -- Received: from relay12.mail.ox.ac.uk (relay12.mail.ox.ac.uk [129.67.1.163]) 10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6L7dxHO020312 10, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jul 2015 02:40:00 -0500 10, 31 -- Received: from hub01.nexus.ox.ac.uk ([163.1.154.218] helo=HUB01.ad.oak.ox.ac.uk) 10, 31 -- by relay12.mail.ox.ac.uk with esmtp (Exim 4.80) 10, 31 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 10, 31 -- id 1ZHS9f-0006Yx-dj 10, 31 -- for Microscopy-at-microscopy.com; Tue, 21 Jul 2015 08:39:59 +0100 10, 31 -- Received: from MBX03.ad.oak.ox.ac.uk ([169.254.3.49]) by HUB01.ad.oak.ox.ac.uk 10, 31 -- ([163.1.154.92]) with mapi id 14.03.0169.001; Tue, 21 Jul 2015 08:39:59 +0100 10, 31 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 10, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 31 -- Subject: RE: [Microscopy] *sort-of commercial post - a kickstarter about 10, 31 -- lights* 10, 31 -- Thread-Topic: [Microscopy] *sort-of commercial post - a kickstarter about 10, 31 -- lights* 10, 31 -- Thread-Index: AQHQw4Blr4OvHsrssUGhlEpGwQ2JBJ3liLo8 10, 31 -- Date: Tue, 21 Jul 2015 07:39:58 +0000 10, 31 -- Message-ID: {168A63C1140FEB4EB61870A513446A4A1885FF22-at-MBX03.ad.oak.ox.ac.uk} 10, 31 -- References: {201507210642.t6L6gI4P009164-at-ns.microscopy.com} 10, 31 -- In-Reply-To: {201507210642.t6L6gI4P009164-at-ns.microscopy.com} 10, 31 -- Accept-Language: en-GB, en-US 10, 31 -- Content-Language: en-GB 10, 31 -- X-MS-Has-Attach: 10, 31 -- X-MS-TNEF-Correlator: 10, 31 -- x-originating-ip: [172.16.150.238] 10, 31 -- Content-Type: text/plain; charset="iso-8859-1" 10, 31 -- MIME-Version: 1.0 10, 31 -- Content-Transfer-Encoding: 8bit 10, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6L7dxHO020312 ==============================End of - Headers==============================
From mike.newsletter30yfrae-at-gmail.com Tue Jul 21 14:30:12 2015 Return-Path: {mike.newsletter30yfrae-at-gmail.com} Received: from gmail.com ([175.196.230.246]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t6LJU9rc028222 for {microscopylistserverarchive5-at-microscopy.com} ; Tue, 21 Jul 2015 14:30:10 -0500 Message-ID: {6842D45F.AB6A819B-at-gmail.com}
Dear Colleagues and Friends,
The Society for Ultrastructural Pathology is organizing its XVIII Congress. This time it will be held in Lisbon, Portugal from July 11 to 15, 2016.
The Congress will be preceded by an introductory course on Ultrastructural Pathology organized by our senior and experienced Colleague Joseph Lloreta-Trull, from Hospital del Mar in Barcelona.
This Congress will focus primarily in ultrastructural diagnosis. Sessions of this applied branch of ultrastructural pathology will be dedicated to the main diagnostic areas for which electron microscopy is important.
A comprehensive set of sessions and presentations will cover both more fundamental research and new technical advances, including super-resolution microscopy, important for Ultrastructural Pathology as a whole.
It is our great pleasure to invite you to join us at UltrapathXVIII in the pleasant, friendly and inexpensive environment of Lisbon, and in the superb facilities of the Gulbenkian Foundation, where the Congress will be held.
Further information about this meeting can be found in the Congress web site Congress-at-UltrapathXVIII.org
On behalf of the organizing committee, I am looking forward to welcoming you to Lisbon in June 2016.
With kind regards,
From the Organizing Committee,
A.P. Alves de Matos Chair of UltrapathXVIII
==============================Original Headers============================== 13, 34 -- From apamatos-at-gmail.com Wed Jul 22 17:59:09 2015 13, 34 -- Received: from mail-wi0-f174.google.com (mail-wi0-f174.google.com [209.85.212.174]) 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6MMx8fH009846 13, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jul 2015 17:59:09 -0500 13, 34 -- Received: by wibxm9 with SMTP id xm9so183833492wib.0 13, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jul 2015 15:59:08 -0700 (PDT) 13, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 34 -- d=gmail.com; s=20120113; 13, 34 -- h=to:from:subject:message-id:date:user-agent:mime-version 13, 34 -- :content-type:content-transfer-encoding; 13, 34 -- bh=Fpgtr5Q3M4Q15lW9nQ5MFmB8k/TVhX9HJIeuYmYogfM=; 13, 34 -- b=fRUeFC66acnDeZ4TNuX9DLcL5FSP1QBX7qHXXIzAWWJRYQfEmUV6ffHSxvjwB2h8eh 13, 34 -- QGaNDDCAAfq0eNV474koPLeuLxRkREcnJUebejCzPhInWj6Pdafzo/lM/qwEm2N1EiSe 13, 34 -- tWHSKG+fF3XmjWUx+Jt8QoQfGA0kEHRhEZ94X47+9Gc+JlLJ+OhQb64hfYKOWWtrrdWb 13, 34 -- 3ewKf7FV0wW6UyI67HqfPrnRV/iGQpVpWUvBUv9nL8Kf4bssnw8kH8hxBOI797OZ+BAl 13, 34 -- I/RYOBksUe0Yduqrr7ROchLV3PT+AsjuDp9BYiA7f6Stdl15OtC1MjxOYBWG8dFsQjNf 13, 34 -- +Rjg== 13, 34 -- X-Received: by 10.194.120.198 with SMTP id le6mr9434601wjb.133.1437605948240; 13, 34 -- Wed, 22 Jul 2015 15:59:08 -0700 (PDT) 13, 34 -- Received: from [192.168.1.79] ([188.250.177.178]) 13, 34 -- by smtp.gmail.com with ESMTPSA id js3sm4570121wjc.5.2015.07.22.15.59.07 13, 34 -- for {Microscopy-at-microscopy.com} 13, 34 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 34 -- Wed, 22 Jul 2015 15:59:07 -0700 (PDT) 13, 34 -- To: Microscopy-at-microscopy.com 13, 34 -- From: AP Alves de Matos {apamatos-at-gmail.com} 13, 34 -- Subject: Society for Ultrastructural Pathology Congress - Ultrapath XVIII 13, 34 -- Message-ID: {55B0203A.6020400-at-gmail.com} 13, 34 -- Date: Wed, 22 Jul 2015 23:59:06 +0100 13, 34 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101 13, 34 -- Thunderbird/38.1.0 13, 34 -- MIME-Version: 1.0 13, 34 -- Content-Type: text/plain; charset=utf-8; format=flowed 13, 34 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Congress will be preceded by an introductory course on Ultrastructural Pathology organized by our senior and experienced Colleague Joseph Lloreta-Trull, from Hospital del Mar in Barcelona.
This Congress will focus primarily in ultrastructural diagnosis. Sessions of this applied branch of ultrastructural pathology will be dedicated to the main diagnostic areas for which electron microscopy is important.
A comprehensive set of sessions and presentations will cover both more fundamental research and new technical advances, including super-resolution microscopy, important for Ultrastructural Pathology as a whole.
It is our great pleasure to invite you to join us at UltrapathXVIII in the pleasant, friendly and inexpensive environment of Lisbon, and in the superb facilities of the Gulbenkian Foundation, where the Congress will be held.
Further information about this meeting can be found in the Congress web site Congress.UltrapathXVIII.org
On behalf of the organizing committee, I am looking forward to welcoming you to Lisbon in June 2016.
With kind regards,
From the Organizing Committee,
A.P. Alves de Matos Chair of UltrapathXVIII
==============================Original Headers============================== 9, 35 -- From apamatos-at-gmail.com Wed Jul 22 18:25:07 2015 9, 35 -- Received: from mail-wi0-f173.google.com (mail-wi0-f173.google.com [209.85.212.173]) 9, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6MNP7xn031476 9, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jul 2015 18:25:07 -0500 9, 35 -- Received: by wibud3 with SMTP id ud3so684752wib.1 9, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jul 2015 16:25:06 -0700 (PDT) 9, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 35 -- d=gmail.com; s=20120113; 9, 35 -- h=to:from:subject:message-id:date:user-agent:mime-version 9, 35 -- :content-type:content-transfer-encoding; 9, 35 -- bh=KeLYCyb82Bsd8MPReZ1lWmN/xZ6BcB7oeHpaam7yang=; 9, 35 -- b=0ts5GYeyUirWMajYyw6gwsVf9gR6ntvyL2TFDmqsH5he2b+/hvgITaUv97wjtyKs/r 9, 35 -- ZnmysOnukJRlEQySKVJIxbbUmlrqGQagYSOOGrbUbYzRuA00MJA6d3JYE6IdP4eGI49q 9, 35 -- e37rGcTDUPL2EKhLVxz3s+h/1ZNucJhH/zL/yGPplr4hGT7r0c3B6dV+ukE6YeXy7sRS 9, 35 -- oc/UnEfmkPXw7b8OyUcN1cWVapoh3mxffepop8QY7Ge9lCLWWBGcRQcuAHWF0lSt6pAV 9, 35 -- s6VeztGIRHQlBpOCy3FAXeLSBPgGc0g/Cgyt1uAnpKY2Jc/cWxM3ca4hYfSm0zc9whcX 9, 35 -- a1qQ== 9, 35 -- X-Received: by 10.180.75.243 with SMTP id f19mr33887989wiw.52.1437607506818; 9, 35 -- Wed, 22 Jul 2015 16:25:06 -0700 (PDT) 9, 35 -- Received: from ?IPv6:2001:8a0:f953:e801:211:9ff:feee:2f41? ([2001:8a0:f953:e801:211:9ff:feee:2f41]) 9, 35 -- by smtp.gmail.com with ESMTPSA id ej5sm4615077wjd.22.2015.07.22.16.25.06 9, 35 -- for {Microscopy-at-microscopy.com} 9, 35 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 9, 35 -- Wed, 22 Jul 2015 16:25:06 -0700 (PDT) 9, 35 -- To: Microscopy-at-microscopy.com 9, 35 -- From: AP Alves de Matos {apamatos-at-gmail.com} 9, 35 -- Subject: CORRECTION - Society for Ultrastructural Pathology Congress - 9, 35 -- Ultrapath XVIII 9, 35 -- Message-ID: {55B02651.40403-at-gmail.com} 9, 35 -- Date: Thu, 23 Jul 2015 00:25:05 +0100 9, 35 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101 9, 35 -- Thunderbird/38.1.0 9, 35 -- MIME-Version: 1.0 9, 35 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] EELS and EFTEM Training School October 2015
Message: October 13-16, 2015, Pleasanton, CA USA
This course reviews the basic theory and practice of EELS imaging and analysis in the TEM, but its main emphasis is on practical techniques, optimum deployment of Gatan hardware and software systems, and advanced EELS and EFTEM applications. Some prior experience with EELS, EFTEM, and Gatan systems is recommended, as is a good familiarity with TEM/STEM instrumentation and techniques. By the end of the course, participants can expect to know how best to optimize the performance of their EELS hardware as well as their EELS and EFTEM experimental setups in order to capture and extract the maximum amount of information from their TEM samples.
Topics include:
ÂFundamentals of EELS and energy-filtered imaging in TEM ÂPrinciples of operation of Gatan EFTEM and EELS systems ÂOptimization of EFTEM and EELS data acquisition ÂQuantification of elemental composition ÂOther information provided by EFTEM/EELS and how best to extract it ÂUse of EELS signals to form maps of elemental and chemical composition ÂEFTEM and STEM EELS spectrum imaging techniques ÂIdentification of material phases via EELS fine structure mapping ÂApplications to biological and physical science specimens
From mike.sfsd4f564s6df45dsiapuf-at-gmail.com Fri Jul 24 11:23:52 2015 Return-Path: {mike.sfsd4f564s6df45dsiapuf-at-gmail.com} Received: from gmail.com ([121.168.56.92]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t6OGNo1i021928 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 24 Jul 2015 11:23:51 -0500 Message-ID: {DB7ACEE0.50524E8C-at-gmail.com}
X-from: mike.marko.em-at-gmail.com
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Email: mike.marko.em-at-gmail.com Name: Mike Marko
Organization: Wadsworth Center
Title-Subject: [Filtered] Open permanent position in Cryo-EM
Message: A permanent Staff Scientist position in cryo-EM is available at Wadsworth Center, NY State Department of Health, Albany, NY. We seek applications from highly motivated individuals specializing in various aspects of high-resolution electron microscopy. Responsibilities for this position include the maintenance of high-end electron microscopes and ancillary equipment, cryo-specimen preparation, data collection, and management. The new hire will be expected to actively support grant-funded projects and the development of new grant applications. Salary will be commensurate with experience.
We have excellent institutional support and infrastructure, including a state-of-the-art JEOL 3200 FSC/PP microscope with an in-column energy filter and a K2 Summit direct-electron detector camera. Interested applicants should submit a cover letter describing their relevant experience, curriculum vitae, and contact information for at least three references to wcphgc-at-health.ny.gov, with "3D-EM" in the subject line. Applications will be accepted until the position is filled and reviewed as they are received. AA/EOE.
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
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Message: Hi everybody, We have a problem down here at the University of Texas at El Paso. Some careless user, who has forfeited his rights to the tool, has uninstalled the PC-SEM on the computer for a Hitachi S-4800. To our knowledge the tool isn't damaged, but we cant recover the software. Does anyone have the PC_SEM software for a Hitachi S-4800 or do they know where to get it. We would be willing to cover any charges if you mail an installation package to us. We have great tool indisposed right now acting essentially as an expensive showpiece. If anyone can help that would be greatly appreciated. E-mail me if there are any additional questions about the computer or the tool.
Thanks in advance, Alejandro Hinojos
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Greetings The 2015 Microscopy and Microanalysis meetings are about a week away and we could still use some volunteers to help with tasks not covered by our student bursaries. If you would like to donate a little time to help keep things running smoothly, please contact me or check by the Volunteer Office during the meetings.
Thanks and see everyone in Portland,
Amanda
Amanda Lawrence Outreach Coordinator/Research Associate Institute for Imaging and Analytical Technologies Mississippi State University
==============================Original Headers============================== 5, 31 -- From ALawrence-at-i2at.msstate.edu Sat Jul 25 04:28:51 2015 5, 31 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6P9Spm5031728 5, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 25 Jul 2015 04:28:51 -0500 5, 31 -- Received: from maillag01.ad.msstate.edu (maillag01.ad.msstate.edu [130.18.230.70]) 5, 31 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id t6P9SpoV029590 5, 31 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 5, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 25 Jul 2015 04:28:51 -0500 5, 31 -- X-Sender: {} 5, 31 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by 5, 31 -- maillag01.ad.msstate.edu (2002:8212:e646::8212:e646) with Microsoft SMTP 5, 31 -- Server (TLS) id 15.0.913.22; Sat, 25 Jul 2015 04:28:31 -0500 5, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 5, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 5, 31 -- 15.00.0913.011; Sat, 25 Jul 2015 04:28:31 -0500 5, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 5, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 31 -- Subject: M&M meeting help 5, 31 -- Thread-Topic: M&M meeting help 5, 31 -- Thread-Index: AQHQxrvPncqPRo+D00a0Bx/zoLdsWA== 5, 31 -- Date: Sat, 25 Jul 2015 09:28:31 +0000 5, 31 -- Message-ID: {82e1ddfb137741f1b8172b588f1f9e08-at-mail02.ad.msstate.edu} 5, 31 -- Accept-Language: en-US 5, 31 -- Content-Language: en-US 5, 31 -- X-MS-Has-Attach: 5, 31 -- X-MS-TNEF-Correlator: 5, 31 -- x-originating-ip: [130.18.230.93] 5, 31 -- Content-Type: text/plain; charset="iso-8859-1" 5, 31 -- MIME-Version: 1.0 5, 31 -- Content-Transfer-Encoding: 8bit 5, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6P9Spm5031728 ==============================End of - Headers==============================
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Email: 12hy1-at-queensu.ca Name: Hongbing Yu
Organization: Queen's University
Title-Subject: [Filtered] TECNAI OSIRIS
Message: Hello Everyone, We have a FEI TECNAI OSIRIS TEM in our lab. The high tension of the microscope has turned off suddenly without any warning or error information for several times during our operation even thought the vacuum and the supplies were all good. And we are not allowed to turn on high tension after that. Does anyone has similar experience or know what is the reason and how to avoid that? Thanks Your sincerely Hongbing Yu
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==============================Original Headers============================== 12, 30 -- From microscopylistserver-noreply-at-microscopy.com Mon Jul 27 14:32:50 2015 12, 30 -- Received: from znl.com ([206.69.208.20]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6RJWnDf017655 12, 30 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2015 14:32:49 -0500 12, 30 -- Received: from localhost (localhost [127.0.0.1]) 12, 30 -- by znl.com (Postfix) with ESMTP id B48A7817323 12, 30 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2015 14:32:49 -0500 (CDT) 12, 30 -- X-Virus-Scanned: amavisd-new at znl.com 12, 30 -- Received: from znl.com ([127.0.0.1]) 12, 30 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 12, 30 -- with ESMTP id n9fvGenzyeaU for {microscopy-at-microscopy.com} ; 12, 30 -- Mon, 27 Jul 2015 14:32:36 -0500 (CDT) 12, 30 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 12, 30 -- by znl.com (Postfix) with ESMTPA id CFF80817315 12, 30 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2015 14:32:36 -0500 (CDT) 12, 30 -- Subject: Fwd: [Filtered] MicroscopyListserverviaWWW:TECNAI OSIRIS HT Problem 12, 30 -- References: {201507271608.t6RG8V6k002948-at-ns.microscopy.com} 12, 30 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 30 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 30 -- From: MicroscopyListserver-NoReply 12, 30 -- {microscopylistserver-noreply-at-microscopy.com} 12, 30 -- X-Forwarded-Message-Id: {201507271608.t6RG8V6k002948-at-ns.microscopy.com} 12, 30 -- Message-ID: {55B68754.1030908-at-microscopy.com} 12, 30 -- Date: Mon, 27 Jul 2015 14:32:36 -0500 12, 30 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 12, 30 -- Gecko/20100101 Thunderbird/38.1.0 12, 30 -- MIME-Version: 1.0 12, 30 -- In-Reply-To: {201507271608.t6RG8V6k002948-at-ns.microscopy.com} 12, 30 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 30 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Could you elaborate what do you mean by saying "we are not allowed to turn on high tension after that?" Do you mean that functionality is disabled, or do you mean that somebody forbid you to do so?
Valery Ray - also with AIM Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
On 7/27/2015 3:36 PM, mail wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: 12hy1-at-queensu.ca } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: 12hy1-at-queensu.ca } Name: Hongbing Yu } } Organization: Queen's University } } Title-Subject: [Filtered] TECNAI OSIRIS } } Message: Hello Everyone, } We have a FEI TECNAI OSIRIS TEM in our lab. The high tension of the microscope has turned off } suddenly without any warning or error information for several times during our operation even } thought the vacuum and the supplies were all good. And we are not allowed to turn on high tension } after that. Does anyone has similar experience or know what is the reason and how to avoid that? } Thanks } Your sincerely } Hongbing Yu } } } Login Host: 130.15.32.247 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
I would first check the SF6 pressure in the gun to make sure it is 6 bars. You can also look at the HTI board located in the Power Cabinet to check for indicator LEDs. Let me know if any of the LEDs are on and I can tell you possible issues. Did you hear any sounds just before the high tension shut off.
John
-----Original Message----- X-from: mail [mailto:mail-at-ns.microscopy.com] Sent: Monday, July 27, 2015 4:17 PM To: John J. Schreiber
X-from: 12hy1-at-queensu.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: 12hy1-at-queensu.ca Name: Hongbing Yu
Organization: Queen's University
Title-Subject: [Filtered] TECNAI OSIRIS
Message: Hello Everyone, We have a FEI TECNAI OSIRIS TEM in our lab. The high tension of the microscope has turned off suddenly without any warning or error information for several times during our operation even thought the vacuum and the supplies were all good. And we are not allowed to turn on high tension after that. Does anyone has similar experience or know what is the reason and how to avoid that? Thanks Your sincerely Hongbing Yu
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==============================Original Headers============================== 12, 30 -- From microscopylistserver-noreply-at-microscopy.com Mon Jul 27 14:32:50 2015 12, 30 -- Received: from znl.com ([206.69.208.20]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6RJWnDf017655 12, 30 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2015 14:32:49 -0500 12, 30 -- Received: from localhost (localhost [127.0.0.1]) 12, 30 -- by znl.com (Postfix) with ESMTP id B48A7817323 12, 30 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2015 14:32:49 -0500 (CDT) 12, 30 -- X-Virus-Scanned: amavisd-new at znl.com 12, 30 -- Received: from znl.com ([127.0.0.1]) 12, 30 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 12, 30 -- with ESMTP id n9fvGenzyeaU for {microscopy-at-microscopy.com} ; 12, 30 -- Mon, 27 Jul 2015 14:32:36 -0500 (CDT) 12, 30 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 12, 30 -- by znl.com (Postfix) with ESMTPA id CFF80817315 12, 30 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jul 2015 14:32:36 -0500 (CDT) 12, 30 -- Subject: Fwd: [Filtered] MicroscopyListserverviaWWW:TECNAI OSIRIS HT Problem 12, 30 -- References: {201507271608.t6RG8V6k002948-at-ns.microscopy.com} 12, 30 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 30 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 30 -- From: MicroscopyListserver-NoReply 12, 30 -- {microscopylistserver-noreply-at-microscopy.com} 12, 30 -- X-Forwarded-Message-Id: {201507271608.t6RG8V6k002948-at-ns.microscopy.com} 12, 30 -- Message-ID: {55B68754.1030908-at-microscopy.com} 12, 30 -- Date: Mon, 27 Jul 2015 14:32:36 -0500 12, 30 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 12, 30 -- Gecko/20100101 Thunderbird/38.1.0 12, 30 -- MIME-Version: 1.0 12, 30 -- In-Reply-To: {201507271608.t6RG8V6k002948-at-ns.microscopy.com} 12, 30 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 30 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have a TIFF file that Iâd like to import into Digital Micrograph using the File } } Import Data command. I know the pixel size of my image but Iâm not sure about the type of the data (integer, RGB, etc.). I have a grayscale micrograph that I would like to convert to a DM3 format to run all my plugins in DM, but I keep getting errors about the import or it appears as distorted noise.
Does anyone have a suggestion for how to interpret the TIFF file format so that it can be imported? Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
==============================Original Headers============================== 7, 26 -- From prvs=643041bbe=steven.spurgeon-at-pnnl.gov Mon Jul 27 18:03:39 2015 7, 26 -- Received: from Emailgw01.pnnl.gov (emailgw01.pnnl.gov [192.101.109.61]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6RN3daL021118 7, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Jul 2015 18:03:39 -0500 7, 26 -- Received: from ex10cashub06.pnnl.gov ([130.20.248.212]) 7, 26 -- by Emailgw01.pnnl.gov with ESMTP/TLS/AES128-SHA; 27 Jul 2015 16:03:34 -0700 7, 26 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.94]) by EX10CASHUB06.pnnl.gov 7, 26 -- ([130.20.248.212]) with mapi id 14.03.0248.002; Mon, 27 Jul 2015 16:03:35 7, 26 -- -0700 7, 26 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} 7, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 26 -- Subject: Importing TIFF to Digital Micrograph 7, 26 -- Thread-Topic: Importing TIFF to Digital Micrograph 7, 26 -- Thread-Index: AQHQyMB2SrmEdjPI4UGMY0TioLaRKQ== 7, 26 -- Date: Mon, 27 Jul 2015 23:03:33 +0000 7, 26 -- Message-ID: {30976D49-5092-4C0D-8B5D-E8FDCDE3B743-at-pnnl.gov} 7, 26 -- Accept-Language: en-US 7, 26 -- Content-Language: en-US 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- x-originating-ip: [130.20.128.10] 7, 26 -- Content-Type: text/plain; charset="utf-8" 7, 26 -- Content-ID: {3922CF132A125640999F508729025A09-at-pnnl.gov} 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t6RN3daL021118 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dsigeti-at-gmail.com Tue Jul 28 00:32:41 2015 Return-Path: {mike.sfsd4f564s6df45dsigeti-at-gmail.com} Received: from gmail.com ([14.48.129.38]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t6S5WcEU025443 for {microscopylistserverarchive5-at-microscopy.com} ; Tue, 28 Jul 2015 00:32:40 -0500 Message-ID: {F4275F21.A798FF6B-at-gmail.com}
Assistant Research Scientist (JOB# 11220) Arizona State University LeRoy Eying Center for Solid State Science http://le-csss.asu.edu/node/355
The LeRoy Eyring Center for Solid State Science (LE-CSSS) at Arizona State University invites applications for an Assistant Research Scientist to work in the John M. Cowley Center for High Resolution Electron Microscopy. This is a full-time, benefits eligible, non-tenure track position renewable on an annual basis contingent upon satisfactory performance, availability of resources, and the needs of the university. Anticipated start date is September 28, 2015.
The successful candidate will be expected to operate a state-of-the-art Electron Microprobe, working in an interdisciplinary research environment, assisting users from many academic departments, including those within the College of Liberal Arts and Sciences and the Fulton School of Engineering. Duties will primarily include operation, troubleshooting and maintaining the electron microprobe, training others to operate the microprobe independently. Opportunities exist for collaborative within these academic units including the School of Earth and Space Exploration and independent research.
Minimum qualifications include: a Ph.D. in Geological Sciences, Chemistry, Materials Science, or a related field by the time of appointment; expertise with running an electron microprobe, expertise in processing micro-analytical data and preparing samples for electron microprobe analysis. Candidates must demonstrate effective verbal and written communication and the ability to work with a large number of scientists. Candidate must demonstrate the ability to develop new microprobe techniques and applications.
Desired qualifications include: demonstrated expertise in use of an electron microprobe to analyze chemically complex materials, expertise with analytical software (Donovan's Probe for EPMA) and an active research program in earth sciences, planetary sciences, solid state chemistry or materials science.
To apply, please send a single pdf file containing a letter of application, a CV with publication list, and the names and contact information of three professional references to csss-at-asu.edu. Electronic applications are strongly encouraged, but hard copy applications will be considered if sent to:
Chair of the Electron Microprobe Search Committee LeRoy Eyring Center for Solid State Science, PO Box 8717014, Arizona State University, Tempe, AZ 85287-1704
Initial review of applications will begin August 10, 2015; if not filled, applications will be reviewed weekly thereafter until the search is closed. Background check is required for employment.
Arizona State University is a VEVRAA Federal Contractor and an Equal Opportunity/Affirmative Action Employer. All qualified applicants will be considered without regard to race, color, sex, religion, national origin, disability, protected veteran status, or any other basis protected by law. https://www.asu.edu/aad/manuals/acd/acd401.html https://www.asu.edu/titleIX/
==============================Original Headers============================== 14, 35 -- From John.Mardinly-at-asu.edu Tue Jul 28 17:06:49 2015 14, 35 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 14, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6SM6mtU029045 14, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Jul 2015 17:06:48 -0500 14, 35 -- X-ASG-Debug-ID: 1438121206-05202e0fe0b93b0001-FOsErg 14, 35 -- Received: from exhubt03.asurite.ad.asu.edu (exhubt03.asurite.ad.asu.edu [129.219.103.42]) by bcnet6.asu.edu with ESMTP id pMWHIzzGUzpEcDsX (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Tue, 28 Jul 2015 15:06:47 -0700 (MST) 14, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 14, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.103.42 14, 35 -- X-ASG-Whitelist: Client 14, 35 -- Received: from EXMBW01.asurite.ad.asu.edu ([169.254.8.191]) by 14, 35 -- exhubt03.asurite.ad.asu.edu ([129.219.103.42]) with mapi id 14.03.0210.002; 14, 35 -- Tue, 28 Jul 2015 15:06:46 -0700 14, 35 -- From: John Mardinly {John.Mardinly-at-asu.edu} 14, 35 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 14, 35 -- Subject: Electron Microprobe Position at Arizona State University 14, 35 -- Thread-Topic: Electron Microprobe Position at Arizona State University 14, 35 -- X-ASG-Orig-Subj: Electron Microprobe Position at Arizona State University 14, 35 -- Thread-Index: AdDJgbGi858k8U3sT66TlblcTZ45lQ== 14, 35 -- Date: Tue, 28 Jul 2015 22:06:46 +0000 14, 35 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9401F369CF1-at-exmbw01.asurite.ad.asu.edu} 14, 35 -- Accept-Language: en-US 14, 35 -- Content-Language: en-US 14, 35 -- X-MS-Has-Attach: 14, 35 -- X-MS-TNEF-Correlator: 14, 35 -- x-originating-ip: [129.219.4.240] 14, 35 -- Content-Type: text/plain; charset="us-ascii" 14, 35 -- MIME-Version: 1.0 14, 35 -- X-Barracuda-Connect: exhubt03.asurite.ad.asu.edu[129.219.103.42] 14, 35 -- X-Barracuda-Start-Time: 1438121207 14, 35 -- X-Barracuda-Encrypted: AES128-SHA 14, 35 -- X-Barracuda-URL: https://129.219.117.248:443/cgi-mod/mark.cgi 14, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu 14, 35 -- X-Barracuda-BRTS-Status: 1 14, 35 -- Content-Transfer-Encoding: 8bit 14, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6SM6mtU029045 ==============================End of - Headers==============================
From advertise.bz222ev-at-gmail.com Tue Jul 28 20:59:52 2015 Return-Path: {advertise.bz222ev-at-gmail.com} Received: from gmail.com ([58.64.164.116]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t6T1xnwo020967 for {microscopylistserverarchive5-at-microscopy.com} ; Tue, 28 Jul 2015 20:59:51 -0500 Message-ID: {F5FCA1F7.409A1CB5-at-gmail.com}
Hello all,
I wonder if there is any historical information in the EM community regarding polymerization of epoxy resin at temperatures lower than 60-70°C. Is it possible to get there by modifying the composition? One would expect so, but expectations are not always workable or realistic.⌠I know I could go the acrylate path.
Thank you,
Jan Leunissen Dunedin, New Zealand
==============================Original Headers============================== 6, 35 -- From leunissen-at-aurion.nl Wed Jul 29 23:03:08 2015 6, 35 -- Received: from nm24.access.bullet.mail.gq1.yahoo.com (nm24.access.bullet.mail.gq1.yahoo.com [216.39.62.55]) 6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6U42iCr019220 6, 35 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jul 2015 23:03:08 -0500 6, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1438228962; bh=KuyItTiysRzbxnQ8sQo2kQJCFY54tfl1oo7Od6+T+kg=; h=From:Subject:Date:To:From:Subject; b=n+5DbFE0BddSIQ6zL4/Cy/tkNViQ5OB+YljQNtAcD27j+8cDpdJPzlxk9VwtBFSb5MnJy1pOrXZpxd7QCmKi5TsDAydNVrSHzgPC+wEPekYA7vhMplWPkqJsl1gyvxxOu0tArTx+mmqLp+MDLY95RPilpEB+npkFscIP17aE6qhIw/tfg7IP8kOfLKsXS7UdePjUTH+U8xBk3fP58dZinVw1Vl5gsnAQnSBecthHhfPdwPAprma6MqaWbjXL7GmgmXp9apdQYA2Y8I9AYWnaKuYN2hq6sgdA1CpKSJzQ4pVkNhVbDoxCfX46Bl0x21PfhZBqpW+OOUO0fY8O083AJg== 6, 35 -- Received: from [216.39.60.166] by nm24.access.bullet.mail.gq1.yahoo.com with NNFMP; 30 Jul 2015 04:02:42 -0000 6, 35 -- Received: from [124.108.96.24] by tm2.access.bullet.mail.gq1.yahoo.com with NNFMP; 30 Jul 2015 04:02:42 -0000 6, 35 -- Received: from [127.0.0.1] by omp1001.tnz.mail.aue.yahoo.com with NNFMP; 30 Jul 2015 04:02:42 -0000 6, 35 -- X-Yahoo-Newman-Id: 160005.47331.bm-at-omp1001.tnz.mail.aue.yahoo.com 6, 35 -- Received: (qmail 89894 invoked by uid 1000); 30 Jul 2015 04:02:42 -0000 6, 35 -- Received: from 124.108.96.72 by rel110.mail.aue.yahoo.com with SMTP; Thu, 30 Jul 2015 04:02:41 +0000 6, 35 -- Received: (qmail 99155 invoked from network); 30 Jul 2015 04:02:41 -0000 6, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1438228961; bh=KuyItTiysRzbxnQ8sQo2kQJCFY54tfl1oo7Od6+T+kg=; h=From:Content-Type:Content-Transfer-Encoding:Subject:Message-Id:Date:To:Mime-Version; b=BHXOKQNGQYnXC8+IzI5WMef2VRSflpI4v29Dr7j2fAnc+K6ep+bepAH0HZk3ySIm5i36nj4qIu3xywPbTY/pIw6WifqObBMhT70O7znGs9GfUSCpCSJ3oKEtCxtIdQwrVeMEdtn1/2upadpqeI8pVMdFlrGz0UVt+k8F0TG55ps= 6, 35 -- X-Yahoo-Newman-Property: ymail-3 6, 35 -- X-YMail-OSG: Tf3t0xYVM1mrH8frIY_wzo3GCNAOsItwBiT2fpwjZLORdkf 6, 35 -- 4EMUKoEdGbkNoATuNM8U7fpece.rhrr6u9MqfTyUq9wrTyxbWs1D8_InAKAA 6, 35 -- QQW9Bm986fjCIvVoDE4hVxq9yLkQ1hWgzgzM7gijqUYpoZEenxUMdsJbgkPS 6, 35 -- Pue18TR3LQQvZK.u77xcZlAcwgWIFY7LeQ7R6JrG4cqIE1QYT0S509uHKPN6 6, 35 -- sZNDTFsStNWeVPGxVD3kcO9vwiobZDlL5LFIlfsEKeZsppW_GASUOs89x7vy 6, 35 -- PbessA_kJPoK.YezPwZCLgP8N__ALH8L6.ClUIe8bnnI1.vjUQ94jd08MQYN 6, 35 -- B8Z1X5C6SDunTzdzNqnOdIKZt1InpC0ZaQqdW5mun3lgLDFSKLajLHjcQja_ 6, 35 -- WQobxfcbuu1BIFxPah6GeX0oyS7EL0b1unDFpbYJDJwf6Celh8wq4tOuL3oY 6, 35 -- 776Yo4L7TIwHlDVh_I2e2lR5zSFHE4oMcd_VgR8kYVaIzZHYmRHbC8LfMLpB 6, 35 -- XHGM0TMgEs5mpjKrRhPB.Hfbpdnhux.OithaJeYs- 6, 35 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 6, 35 -- From: Jan Leunissen {leunissen-at-aurion.nl} 6, 35 -- Content-Type: text/plain; charset=utf-8 6, 35 -- Subject: epoxy polymerisation at lower temperatures 6, 35 -- Message-Id: {655ED07B-6CDB-4585-B033-81ACD0005395-at-aurion.nl} 6, 35 -- Date: Thu, 30 Jul 2015 16:02:39 +1200 6, 35 -- To: microscopy-at-microscopy.com 6, 35 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2102\)) 6, 35 -- X-Mailer: Apple Mail (2.2102) 6, 35 -- Content-Transfer-Encoding: 8bit 6, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6U42iCr019220 ==============================End of - Headers==============================
From benny0439655322345345ykier-at-gmail.com Thu Jul 30 02:24:19 2015 Return-Path: {benny0439655322345345ykier-at-gmail.com} Received: from gmail.com ([14.48.129.38]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t6U7OG1h012539 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 30 Jul 2015 02:24:18 -0500 Message-ID: {D21E4B43.9322EA42-at-gmail.com}
Thank you all for your answers, the list provides such a wealth of knowledge.
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Email: tom.mcnulty-at-rigaku.com Name: tom mcnulty
Organization: rigaku americas corporation
Title-Subject: [Filtered] employment
Message: Rigaku Americas Corporation, a leading worldwide supplier of analytical X-ray instrumentation is seeking an XRM Product Specialist for our North American marketing group. This position is a full-time position at our office located at 9009 New Trails Drive, The Woodlands, Texas, 77381.
Qualifications:
 A thorough understanding of NanoCT/XRM principals, instrument design, and applications is required.
 Previous work experience within the Analytical Instrumentation Industry is preferred.
 A graduate degree in mechanical, materials, or bio-medical engineering is preferred.
 Bi-lingual candidates, English/Spanish or English/Japanese, are preferred.
 Exceptional written, verbal, and presentation skills are critical.
Responsibilities:
 Operation, maintenance, training, and demonstration of Rigaku s line of NanoCT/XRM products.
 Technical and product based sales support for our North American sales force and channel sales representatives
 Participation in the development of product marketing strategies for Rigaku NanoCT/XRM products in North America
 Collaboration with manufacturing centers in the US and Japan regarding new product development and product introductions
 The position requires up to 50% travel both inside the US and abroad.
 Other duties as assigned.
Must meet minimum requirements.
Apply to position at: https://careers-rigaku.icims.com
Compensation will consist of both salary and performance based incentive programs commensurate with experience.
Rigaku offers a rewarding work environment and excellent benefits. All applicants are kept in strict confidence. Benefits package including Health, Dental, Vision, STD, LTD, Life insurance, section 125 flexible spending accounts, 401(k) plan and more. Rigaku is an EEO/AA Employer M/F/Disabled/Veteran. Applicants must be able to prove they can legally work in the US
Rigaku will be exhibiting at The 2015 MSA Microscopy & Micro-analysis Conference in Portland OR, Aug 2-6. Qualified candidates are welcome to visit with Rigaku at the conference to discuss this opportunity in person.
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Particle size distribution using Gatan digital micrograph.
Message: How to do particle size distribution using Gatan Digital micrograph? TIA,
Ravi.
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Hi All This yearšs Family Affair for delegates families and friends is Wednesday afternoon August 5.
As well as microscopic explorations of materials and metals and a visitor from NASA, I have a few Foldscopes - origami microscopes.
The Solve the Mystery to take down to the Exhibitoršs Hall Secret Agent, James B Atom, has another message for Headquarters. If you remember last year he wrote it on a Focused Ion Beam so that it could only be read using a scanning electron microscope. The message last year was łNever Trust and Atom, They make up everything.˛ This yearšs task should you choose to accept it is another message written on a FIB in four pieces. When you have read it, you take your piece to Headquarters (the Outreach Booth) where it will be assembled on a white board.
Best wishes Elaine
Dr. Elaine C. Humphrey Lab Manager and STEHM Technologist Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada Lab: 250-853-3968 cell: 250-886-2068 website: http://www.stehm.uvic.ca
==============================Original Headers============================== 10, 36 -- From ech-at-uvic.ca Fri Jul 31 16:29:00 2015 10, 36 -- Received: from armhook.comp.uvic.ca (armhook.comp.uvic.ca [142.104.177.229]) 10, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t6VLT0sc025605 10, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Jul 2015 16:29:00 -0500 10, 36 -- Received: from tailor.uvic.ca (tailor.uvic.ca [142.104.225.130]) 10, 36 -- by armhook.comp.uvic.ca (8.14.4/8.14.3) with ESMTP id t6VLSv08005994 10, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Jul 2015 14:28:57 -0700 10, 36 -- Received: from lyretail.uvic.ca (142.104.225.129) by tailor.uvic.ca 10, 36 -- (142.104.225.130) with Microsoft SMTP Server (TLS) id 15.0.1076.9; Fri, 31 10, 36 -- Jul 2015 14:28:56 -0700 10, 36 -- Received: from lyretail.uvic.ca ([fe80::a175:af10:b91b:4534]) by 10, 36 -- lyretail.uvic.ca ([fe80::a175:af10:b91b:4534%13]) with mapi id 10, 36 -- 15.00.1076.000; Fri, 31 Jul 2015 14:28:57 -0700 10, 36 -- From: Elaine Humphrey {ech-at-uvic.ca} 10, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 36 -- Subject: Family Affair M&M 2015 10, 36 -- Thread-Topic: Family Affair M&M 2015 10, 36 -- Thread-Index: AQHQy9fnLpm7Z30JmUS+7knkBEnoZA== 10, 36 -- Date: Fri, 31 Jul 2015 21:28:56 +0000 10, 36 -- Message-ID: {D1E136A3.503F9%ech-at-uvic.ca} 10, 36 -- Accept-Language: en-US 10, 36 -- Content-Language: en-US 10, 36 -- X-MS-Has-Attach: 10, 36 -- X-MS-TNEF-Correlator: 10, 36 -- user-agent: Microsoft-MacOutlook/14.4.3.140616 10, 36 -- x-ms-exchange-transport-fromentityheader: Hosted 10, 36 -- x-originating-ip: [142.104.193.193] 10, 36 -- Content-Type: text/plain; charset="iso-8859-1" 10, 36 -- Content-ID: {8A639A6B7A3BB34F8E4F6C1DB96DE44B-at-local.uvic.ca} 10, 36 -- MIME-Version: 1.0 10, 36 -- X-UVic-Virus-Scanned: OK - Passed virus scan by ClamAV (clamd) on armhook 10, 36 -- X-UVic-Virus-Scanned: OK - Passed virus scan by Sophos (sophie) on armhook 10, 36 -- X-UVic-Scan: armhook.comp.uvic.ca filter_version 3.7.9 10, 36 -- X-Scanned-By: MIMEDefang 2.67 on 142.104.177.245 10, 36 -- Content-Transfer-Encoding: 8bit 10, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t6VLT0sc025605 ==============================End of - Headers==============================
From news3409384mxfh-at-gmail.com Sat Aug 1 16:54:20 2015 Return-Path: {news3409384mxfh-at-gmail.com} Received: from gmail.com ([211.192.221.35]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t71LsHsf022658 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 1 Aug 2015 16:54:19 -0500 Message-ID: {0602570E.54CECBE9-at-gmail.com}
X-from: gary-at-gaugler.com
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I'd really like to find some LEO/Zeiss users who are way more versed in SmartSEM macros than myself. I'm trying to make a macro to take scans with scan speed and line integration values according to specific store resolutions. This seemed pretty straightforward but it got real problematic quickly.
Image storing is using line integration for noise reduction, and depending on the store resolution, the scan speed and line integration N are changed to result in about a one minute total scan time for each store resolution. The macro I have thus far is:
Macro Name : photo2 Version 1.0 /* GDG July 2015 */ Unfreeze All Freeze on = End Frame If : Store resolution = 3072 * 2304 Is True Line Int. N= 8 Scan Speed 5 EndIf Statement If : Store resolution = 2048 * 1536 Is True Line Int. N= 6 Scan Speed 6 EndIf Statement If : Store resolution = 1024 * 768 Is True Line Int. N= 7 Scan Speed 8 EndIf Statement If : Store resolution = 512 * 384 Is True Line Int. N= 12 Scan Speed 9 EndIf Statement End Macro PHOTO2
I have a drop down Menu for Store Resolution but I cannot figure how to feed this into the macro before the first If statement. I tried Else and other decision statements but this macro is close to working. If I set the Store Resolution in the Scanning tab and then just run the macro, it works fine. But I'm looking for a way to specify the resolution at the beginning and have that fed into the Photo2 macro.
Does anyone have any suggestions about how to accomplish this task? All inputs are appreciated. TIA.
gary g.
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
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Email: gcat-at-udel.edu Name: Glenn
Organization: University of Delaware
Title-Subject: [Filtered] ISI-DS-130 service manual request
Message: Hi,
Does anyone have an electronic copy of the service manual for an ISI-DS-130 scanning electron microscope? Does such a manual exist?
I am interested in tuning up a unit and need to know the reference voltages for all of the test points.
Thanks!
-Glenn
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I have an unusual request for our machine. It seems we've hit more than our fair share of snags with our operating system and LEO software and we're hoping that someone on the listserver can help us out. What we would like to do is get a hold of a hard-drive disc image of a working LEO 1550 that is still using the old/non-upgraded plinth system.
Currently, we are running Windows 98 (ya, really..) and LEO 32 software version 2.03, patch f. We had a hard drive malfunction on us about 6 months ago and tried to install a replacement from scratch. We were able to get a hold of old IDE drives, install Win98, install LEO-32, but not without a lot of hunting for drivers and updates of files. In the end, the software was seemingly working. We were able to image and run EDS for about a month. Then one day the software crashed and hasn't been the same since.
Sorry for the sob story, but if there's any chance we can get a disc image, any and all sensitive data/photos may be removed (I wouldn't have any need for it I'm sure), thought this shot in the dark couldn't hurt. Also, if you know of any other labs that might have a 1550, that maybe don't subscribe here, please let us know so we can get in touch with them. Of course compensation isn't out of the question, but hopefully of a reasonable manner.
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==============================Original Headers============================== 11, 40 -- From andrew.ornelas-at-intertek.com Thu Aug 6 12:46:02 2015 11, 40 -- Received: from gwo3.mbox.net (gwo3.mbox.net [165.212.64.25]) 11, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t76Hk27X018675 11, 40 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2015 12:46:02 -0500 11, 40 -- Received: from gwo3.mbox.net (localhost [127.0.0.1]) 11, 40 -- by gwo3.mbox.net (Postfix) with ESMTP id 3mnHL203Yvz18X993 11, 40 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2015 17:46:01 +0000 (UTC) 11, 40 -- X-USANET-Received: from gwo3.mbox.net [127.0.0.1] by gwo3.mbox.net via mtad (C8.MAIN.4.02J) 11, 40 -- with ESMTP id 515THFRt41216Mo3; Thu, 06 Aug 2015 17:45:55 -0000 11, 40 -- X-USANET-Routed: 6 gwsout-disclaimer Q:watd 11, 40 -- X-USANET-Routed: 5 gwsout-gwsd Q:gwsd 11, 40 -- X-USANET-Routed: 3 gwsout-vs Q:bmvirus 11, 40 -- X-USANET-GWS2-Service: gwsdout-archive True 11, 40 -- X-USANET-GWS2-Tenant: intertek.com 11, 40 -- X-USANET-GWS2-Tagid: RETN 11, 40 -- Received: from S1P5HUB1.EXCHPROD.USA.NET [165.212.120.254] by gwo3.mbox.net via smtad (C8.MAIN.4.02Q) 11, 40 -- with ESMTPS id XID156THFRt45005Xo3; Thu, 06 Aug 2015 17:45:55 -0000 11, 40 -- X-USANET-Source: 165.212.120.254 OUT andrew.ornelas-at-intertek.com S1P5HUB1.EXCHPROD.USA.NET TLS 11, 40 -- X-USANET-MsgId: XID156THFRt45005Xo3 11, 40 -- Received: from S1P5DDAG1B.EXCHPROD.USA.NET ([169.254.2.90]) by 11, 40 -- S1P5HUB1.EXCHPROD.USA.NET ([10.120.223.31]) with mapi id 14.03.0235.001; Thu, 11, 40 -- 6 Aug 2015 17:45:53 +0000 11, 40 -- From: "Andrew Ornelas Intertek" {andrew.ornelas-at-intertek.com} 11, 40 -- To: "'Microscopy-at-Microscopy.com'" {Microscopy-at-microscopy.com} 11, 40 -- Subject: Help for our Zeiss - LEO 1550 11, 40 -- Thread-Topic: Help for our Zeiss - LEO 1550 11, 40 -- Thread-Index: AdDQbz/oFxCGa+mAQe6nPLZl5qpSXQAAEiwQ 11, 40 -- Date: Thu, 6 Aug 2015 17:45:53 +0000 11, 40 -- Message-ID: {76B37BEE80A38F41BCCD0E40574435E8506D82FF-at-S1P5DDAG1B.EXCHPROD.USA.NET} 11, 40 -- References: {76B37BEE80A38F41BCCD0E40574435E8506D82DD-at-S1P5DDAG1B.EXCHPROD.USA.NET} 11, 40 -- In-Reply-To: {76B37BEE80A38F41BCCD0E40574435E8506D82DD-at-S1P5DDAG1B.EXCHPROD.USA.NET} 11, 40 -- Accept-Language: en-US 11, 40 -- Content-Language: en-US 11, 40 -- X-MS-Has-Attach: 11, 40 -- X-MS-TNEF-Correlator: 11, 40 -- x-originating-ip: [12.184.110.124] 11, 40 -- Content-Type: text/plain; charset="us-ascii" 11, 40 -- MIME-Version: 1.0 11, 40 -- Content-Transfer-Encoding: 8bit 11, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t76Hk27X018675 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dsxheuu-at-gmail.com Fri Aug 7 08:28:27 2015 Return-Path: {mike.sfsd4f564s6df45dsxheuu-at-gmail.com} Received: from gmail.com ([180.150.230.114]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t77DSOUh000444 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 7 Aug 2015 08:28:25 -0500 Message-ID: {1B3B6CD0.14298030-at-gmail.com}
Hello listserv subscribers,
The Nanoscale Characterization and Fabrication Laboratory at Virginia Tech is preparing to auction off our Thermo Fisher Microlab 350 Auger/XPS system. Details of the system are below. We are looking for parties who may be interested in bidding for this unit, so please contact me offline for more details and pictures if the AES is of interest to you. We will also be auctioning a Cameca Atomika SIMS, details of which will be posted to the SIMS listserv.
Description: The Thermo Fisher Scientific (formerly VG Scientific) Microlab 350 is a multi-purpose lab instrument with capability for both Auger Electron Spectroscopy (AES) and XPS (X-ray Photoelectron Spectroscopy). The chamber is optimized for Auger analysis â using a field-emission electron source. Sample sizes are limited to about 15mm X 15mm X 7mm due to the sample holders used and the method of introducing samples into the analysis chamber. The system also has a dual-anode (Mg/Al) achromatic X-ray source for XPS on large spots (effective analysis area of several mm X several mm). There is an ion gun for sputter depth profiling, although the ion gun is optimized for Auger and is not for depth profiling for XPS analysis due to the large spot required. The system has a motorized, 5-axis stage. The system has a standard ion pump for pumping the main chamber and is equipped with a turbo pump for pumping the load lock and differentially pumping the Ar gas line.
3. Tool Description: Microlab 350 can perform Auger Electron Spectroscopy (AES) and XPS (X-ray Photoelectron Spectroscopy) or ESCA (Electron Spectroscopy for Chemical Analysis). AES is performed using a field-emission electron source. XPS uses a dual anode (Mg/Al) large spot, achromatic X-ray source.
4. Date of Manufacture: April 2001
5. Entity Code / CEID (if applicable): AES22
6. Sample type (wafer, solid, gas, liquid): Wafer pieces and other solid samples
7. Sample dimensions: Max. size of ~15mm X 15mm X 7mm (thickness)
8. Vendor Serial #A1105
9. Operating System and Software Version: Windows XP Professional and Thermo Avantage 3.51 Build 02148 (Installed 2007)
10. Location: Blacksburg, VA 24060
11. Operational Status: Inoperable when moved into storage. All parts and pieces other than the water chiller are included. It has been over six months since a beam was successfully generated on the system. Vacuum is in good working order and system is currently under vacuum. While we suspect this system can be brought into fully functioning order again, we are advertising this as parts only.
12. Electrical Power Configuration: 230V, 40A, single phase
13. Benchtop or standalone? Standalone
14. Sample Loading Configuration (load-lock, robot, liquid sample introduction system, etc.): Load lock for pumping down and then samples are manually introduced by means of a transfer rod and âwobble stickâ arm
Again, please contact me directly for additional information including space and facilities requirements, pictures, and details regarding the auction process.
Thanks, Chris Winkler
Senior Research Associate Nanoscale Characterization and Fabrication Laboratory Institute for Critical Technology and Applied Science Virginia Tech crwinkler-at-vt.edu (540) 200-9511
==============================Original Headers============================== 21, 29 -- From microwink-at-gmail.com Fri Aug 7 11:50:14 2015 21, 29 -- Received: from mail-ob0-f170.google.com (mail-ob0-f170.google.com [209.85.214.170]) 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t77GoDvn007264 21, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Aug 2015 11:50:14 -0500 21, 29 -- Received: by obbfr1 with SMTP id fr1so46407354obb.1 21, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 07 Aug 2015 09:50:13 -0700 (PDT) 21, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 21, 29 -- d=gmail.com; s=20120113; 21, 29 -- h=mime-version:date:message-id:subject:from:to:content-type 21, 29 -- :content-transfer-encoding; 21, 29 -- bh=xS98kaNU9J7K8p9GwljHlqPYERnXqaWWnTYrJjhwIF8=; 21, 29 -- b=hsQmgc3JseCsIRAGpiRZ1U2t6Hvbo7VWbvP+5PzKX2FtFlJNJkscHLVbqJtqixzA5u 21, 29 -- 35NzNW/Hf52OpiNM7/e000nvKStc3O3fJ2vveODVGvNBtnLG8U1020ad0CPw6N7tTcl5 21, 29 -- MIRiNGIA1qba+ogavskj/NagcMDdPN4aCmvqUI3ACkwyhXqeBNYaKEFNyEmdYKadf7e+ 21, 29 -- JMgqg8IpXV6JLpZ5IH6LEPBM5bmqb2KbW+VGVHBbVuJLOQTWN+91BSsqZL1FY7xZf2nq 21, 29 -- 9Qs7wVGm88WhZ0ghS6D79aRdP4L8luvI+b3jTbCVP3rOxve+rPCbmOsYI2NIsJbfpXgk 21, 29 -- QT5w== 21, 29 -- MIME-Version: 1.0 21, 29 -- X-Received: by 10.60.45.104 with SMTP id l8mr8013360oem.61.1438966213584; Fri, 21, 29 -- 07 Aug 2015 09:50:13 -0700 (PDT) 21, 29 -- Received: by 10.202.83.213 with HTTP; Fri, 7 Aug 2015 09:50:13 -0700 (PDT) 21, 29 -- Date: Fri, 7 Aug 2015 12:50:13 -0400 21, 29 -- Message-ID: {CAA8T2PMX5tTpY4TZBHLRkqyAjsvwv2omjQo7ObfmXTWdgbxfpg-at-mail.gmail.com} 21, 29 -- Subject: Thermo Fisher Scientific Microlab 350 AES/XPS for auction 21, 29 -- From: Christopher Winkler {microwink-at-gmail.com} 21, 29 -- To: Microscopy-at-microscopy.com 21, 29 -- Content-Type: text/plain; charset=UTF-8 21, 29 -- Content-Transfer-Encoding: 8bit 21, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t77GoDvn007264 ==============================End of - Headers==============================
We have an old (circa 2000) LEO 1450VP SEM that we need to get rid of fast. It is in good working condition. We have purchased a new Hitachi SEM and we need to get the old machine out of here. We have 3 options, either find an institution that will buy it from us, at a price to be negotiated, or find a scientific research organization, preferably in an under-developed country, that we could donate it to provided they pay for the shipping. Or we could call a metals recycler and have them haul it away as junk. That seems like a shame since the machine is working.
It has a tungsten filament, turbomolecular pump, has conventional ET SE detector, VPSE and BSD. It has the variable pressure option. The machine has been under a Zeiss service contract until a few months ago. NO analytical instruments, no X-ray EDS, etc. The machine is based on a Win 98 PC. Machine comes with a water chiller. SEM was used exclusively for zoological research.
Direct any inquiries to: Scott Serata California Academy of Sciences SEM Lab 55 Music Concourse Dr San Francisco, CA 94118
scottserata-at-gmail.com (415) 724-4807
Thanks!
==============================Original Headers============================== 5, 26 -- From scottserata-at-gmail.com Fri Aug 7 21:25:00 2015 5, 26 -- Received: from mail-vk0-f46.google.com (mail-vk0-f46.google.com [209.85.213.46]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t782Oxjs010425 5, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Aug 2015 21:24:59 -0500 5, 26 -- Received: by vkfx1 with SMTP id x1so15550967vkf.0 5, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 07 Aug 2015 19:24:59 -0700 (PDT) 5, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 26 -- d=gmail.com; s=20120113; 5, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 5, 26 -- bh=35Lu000vg/N43kZk/tpR5XvAxafWZFbbtRgWf5egI6s=; 5, 26 -- b=toSpUOur/HlpHxmcX/AUQyeRrbzMuxcVg4DRG/cKedRT9bkr9b452XfHRGRhsqKDpn 5, 26 -- EPgEzDVVbEPUZkeeTrho+OH1ZewFjjgdXf+rMNDDSbbQJNv2F3jCcgEqeqnl2DVoacT0 5, 26 -- VsvazAyR57fmKb2n6CBSHHCkHhZIhk48pqsSsGtPXThmy0B9BxFLbZ84yCb47ppoTUlH 5, 26 -- Yz31+7kzDsCSIOLm99B9Xne1CAOKmA4iXATODsTXy1epyXo9ZvYDKhwcpQUVrLIZiw9L 5, 26 -- Dt/egaz8X6Hcr/bSsXSg2M6ptRtDpHCgJuNFeLt8rOjLcx1SUrdfGdQXVJKzTPS/bEiv 5, 26 -- Q0mA== 5, 26 -- MIME-Version: 1.0 5, 26 -- X-Received: by 10.52.113.40 with SMTP id iv8mr11746569vdb.28.1439000699422; 5, 26 -- Fri, 07 Aug 2015 19:24:59 -0700 (PDT) 5, 26 -- Received: by 10.31.186.9 with HTTP; Fri, 7 Aug 2015 19:24:59 -0700 (PDT) 5, 26 -- Date: Fri, 7 Aug 2015 19:24:59 -0700 5, 26 -- Message-ID: {CAGdf6R4F18ZSK74qmhZaRtUN9-k2HVuqD1irWk_wOUL0_JO6Ow-at-mail.gmail.com} 5, 26 -- Subject: Used LEO/Zeiss SEM for sale or possible donation, LEO1450VP, circa 2000 5, 26 -- From: Scott Serata {scottserata-at-gmail.com} 5, 26 -- To: Microscopy-at-microscopy.com 5, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
From hanja07656515151fuia-at-gmail.com Sun Aug 9 03:04:13 2015 Return-Path: {hanja07656515151fuia-at-gmail.com} Received: from gmail.com ([211.104.160.66]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t7984A3v032660 for {microscopylistserverarchive5-at-microscopy.com} ; Sun, 9 Aug 2015 03:04:11 -0500 Message-ID: {A386F5F2.B215F75B-at-gmail.com}
Hello all,
a summary of the answers I received on/off list regarding my question whether it is possible to polymerise epoxy resins at lower temperature than commonly applied.
Thanks everyone, it was good to have the opportunity to discuss this issue at M&M in Portland!
Jan Leunissen, (Back in snowy Dunedin, New Zealand)
original message:
Hello all,
I wonder if there is any historical information in the EM community regarding polymerization of epoxy resin at temperatures lower than 60-70°C. Is it possible to get there by modifying the composition? One would expect so, but expectations are not always workable or realistic.⌠I know I could go the acrylate path.
Thank you,
Jan Leunissen Dunedin, New Zealand
âââ
I have personally used MBond 610 cured at room temperature for manual polished microscopy samples without any problems whatsoever. You only need to keep it glued for at least 24 hours for the resin to cure fully at room temperature. Regards, Debangshu Mukherjee | MatSE PhD Candidate, Penn State MRSEC Center for Nanoscale Science | T: (617) 501-7316 N-303 Millennium Science Complex, University Park, PA
âââ
I always had the opinion that âhistoricalâ (practical) fact finding by the âold guysâ told about the beneficial effect of polymerizing (at least starting polymerization) epoxy resins at lower temperatures. This ended up in the âclassicalâ polymerization of epoxy blocks (EPON 812) 35-37° / 45° / 60-65° for 12 h. each. When I digged into the original publication of LUFT 1961 (*) I found the following:
ââŚ..Moreover, according to Lee and Neville (1957), the amount of cross-linkage can be modified further to some extent by varying the temperature of the curing cycle. Thus low temperatures are said to favor a linear polymer with fewcross-linkages. Lee and Neville state further that once the molecular architecture of the gelled resin is established, it is less likely to be influenced by subsequent higher temperatures, which are, nevertheless, necessary to increase the degree of cure. For purposes of tissue embedding, a cool initial incubation period might be useful to evaporate off the propylene oxide without boiling (b.p. 35-37°C.). In view of the arguments presented by Lee and Neville (1957), a three-stage curing program was chosen with incubation at 35 °, 45 °, and 60°C. for about 12 hours at each temperature. The resulting resin has a low heat distortion point, indicating a low degree of cross-linkage. âŚ.â
I routinely adhered (since starting in 1981 and prior to that during my studies at the University) up to date with the so called âclassical polymerization scheduleâ as well as mixing proportions of LUFT (using the classical SHELL-Monsanto EPON 812 regularly up to 1985, then changing to Glycidether 100 from SERVA as the substitute for the then unavailable EPON 812: 35-37°C at least over night, 45°C at least 12 h, 65°C at least for 12 h adding 1.75% DMP-30 to the resin mixture; but also using the so called ârapid embedding scheduleâ [= dehydration for some reasons after 70% EtOH into 2,2,-DMP acidified, at least 5x for 5 min each, 2,2-DMP acidified : complete resin mixture= 1:1 at least for 2 h on rotator, the resin mixture of which consists of the original LUFT ratio Epon 812/Glycidether100 : DDSA : MNA/NMA BUT + 2% DMP-30, starting polymerization with: 35°C for at least 3-4 hrs [and re-/orienting), 45°C for at least 2 hrs [and re-/orienting at the right time), 65°C for at least 2 hrs [resin has polymerized at that temperature though], and a final 1 h at 80°-85°C without no problems in cutting and/or staining semithin sections or ultrathin sections ).
Donât know if you wanted information about low(er = below 35°C) polymerization temperatures for epoxy resins initially but must admit that I personally never tried (e.g.) âPLTâ with EPON or any other âoldâ Epoxide (EPON 812 substitute) resin (because of the boiling point of PO -at- ca.35°C).
*) John H. LUFT: IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS J Biophys Biochem Cytol 9, -1961, pp.409-414.
NB: by separate e-mail to you I can provide a pdf âEPOXY RESINS & polymerizatn(Overview)by BHATNAGAR MS,1996(13-07-09pdf).pdfâ which I havenât evaluated crucially for regarding our quest but hope that you might find therein some valuable information on polymerization mechanisms.
Hope to have added anything new (or old news) to this your interesting request, which I would know more about if you get personal REâs via the MSA ListserverâŚ.
best wishes and regards
Wolfgang
Wolfgang MUSS SALZBURG AUSTRIA [to retire by 1st of Dec. 2015]
âââ
I use Buehler epothin or Stuers Epofix that both cure at room temp overnight. I have cured epothin in the refrigerator in the past. I have only used these to embed things for sections or SEM, they sure fast enough that there is little infiltration, except of larger voids. Both can be used with vacuum. -David David A. Waugh, Ph.D. Research Assistant Department of Anatomy and Neurobiology NEOMED 4209 State Route 44 Rootstown, OH 44272
âââ
Back in the early â70s we cured by first using a 35ËC oven overnight then into 60Ë to finish off. If we left the blocks in the 35 too long they never really got hard.
Next I had an oven that did not keep temperature very well. It fluctuated between 50 and 60. Iâd leave the real Epon 812 blocks in a full 48 hours and that cut well.
Next came using original Epon, curing at 60Ë and another lab took blocks, sectioned and immuno stained but I do not know the details. It worked!
I am interested to see what others say.
Pat Connelly
âââ
Yes, you can polymerize at a lower temp, just takes longer. If you leave epoxy resin with the accelerator added at room temp it will polymerize in a few days.
Geoff (Mcauliff)
âââ
Yes it is possible to to polymerize epoxy resins below 60-70 C just by doubling the accelerator in the batch of resin used for embedding and polymerizing at 45 C for 48 hrs rather than the usual overnight. There are a number of labs that are polymerizing epoxy resins at 60C overnight and still doing colloidal gold immunolabel after embedding in epoxy resins. I have used epoxy resins based on Quetol 651 for the last 25 years and done immunolabel successfully. It is also possible to increase the anhydride to epoxide ratio (A:E) and get more cross linkage that way but I do not know of anyone who has tried that. It is something that most people are not knowledgable enough to do correctly. I submitted a paper on reviewing the use of Quetol 651 and formulations. I can send you a copy in the future after the paper is accepted.
There are a couple of things that can be done ia addition to lowering the temperature and doubling the accelerator. Brorrson recommended the use of para-phyleneaminediamine (PPD) to the dehydration alcohols which works quite nicely with epoxy or acrylic resins. The epoxies are easier to section and probably not as expensive as Lowicryls.
I am attaching a protocol on the use of PPD and a short paper that I published a couple of years ago in Microscopy Today (only in original off list messageâ Jan)
I know peole who swear that you can only use acrylic resins and must keep the temperature below 60 C; however the experience for some of us is just the opposite. In my lab we have done colloidal gold localization of GFP in a Quetol based epoxy resin.
Hope all this helps you go in the right direction.
E. Ann Ellis Ms, CEMT, FRMS, FMSA Consultant in Biological Electron Microscopy eann.ellisem-at-gmail.com
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==============================Original Headers============================== 36, 35 -- From leunissen-at-aurion.nl Mon Aug 10 15:21:30 2015 36, 35 -- Received: from nm22-vm8.access.bullet.mail.gq1.yahoo.com (nm22-vm8.access.bullet.mail.gq1.yahoo.com [216.39.63.230]) 36, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7AKLTkO023971 36, 35 -- for {microscopy-at-microscopy.com} ; Mon, 10 Aug 2015 15:21:30 -0500 36, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1439238089; bh=m3j6woQvzVxtRQxLW8pfKaTSztdROxUgynKzKgyIn1I=; h=From:Subject:Date:To:From:Subject; b=U3h1tFc9T6TDHZNXpAqxCsA67YSy0uJt9NXn+dIRetTx+6JtL255jdl/Hldx5vwBTdBj/pJWomf+z//NLD2/ecSK9Ry2pwGZ0tIdu2f75Em60yZpsYOy2eomDQaHNROORUM8a5WNBCrzIgGIMrqfQxyx1qz8AR5MlAxPzQK8Y4ImmdozriqnrP1JFRvHYAVSNOjlIM/pe2Hs6Ar8MK20HJkUdYnlXy9uT4Mx7ry+gF81WzZnc310n1XhJt6OuXwH/atx6FFEFP3wVNg5h80SLNJaW0OMS041N4oitKkKdnBaSf65R2ly2IWUptwZM/Cpyvo9hCUXR6w60nFNTl51pw== 36, 35 -- Received: from [216.39.60.172] by nm22.access.bullet.mail.gq1.yahoo.com with NNFMP; 10 Aug 2015 20:21:29 -0000 36, 35 -- Received: from [124.108.96.25] by tm8.access.bullet.mail.gq1.yahoo.com with NNFMP; 10 Aug 2015 20:21:29 -0000 36, 35 -- Received: from [127.0.0.1] by omp1002.tnz.mail.aue.yahoo.com with NNFMP; 10 Aug 2015 20:21:28 -0000 36, 35 -- X-Yahoo-Newman-Id: 800159.73583.bm-at-omp1002.tnz.mail.aue.yahoo.com 36, 35 -- Received: (qmail 43431 invoked by uid 1000); 10 Aug 2015 20:21:28 -0000 36, 35 -- Received: from 124.108.96.119 by rel109.mail.aue.yahoo.com with SMTP; Mon, 10 Aug 2015 20:21:28 +0000 36, 35 -- Received: (qmail 38792 invoked from network); 10 Aug 2015 20:21:28 -0000 36, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1439238088; bh=m3j6woQvzVxtRQxLW8pfKaTSztdROxUgynKzKgyIn1I=; h=From:Content-Type:Content-Transfer-Encoding:Subject:Message-Id:Date:To:Mime-Version; b=HN5f3VZ0zwcAHRDFu8Fk5y1NkCOkT+f4S0Qjnhq7TiURtVJeL2l8S1u4v87WBEX2TjAfgJIC4CpCj7ExRZA0Jn+u7jySa5oDdyc3GqT/GSJBiJnWjHVX2yFy/DEJZLe93TL23dAkm7ZIeSGBgE7qu6LJ/JiXa7xaY1vj6c1PtLw= 36, 35 -- X-Yahoo-Newman-Property: ymail-3 36, 35 -- X-YMail-OSG: YX3ivKIVM1nV32O_.56EiyHtJhGNVsdnX9LvrsnFsKsPZuh 36, 35 -- 1sJ4VA_BSXSy_gIoXQfoKfgkFeWbwTupMlQtxvef9TazKo.Liwq.M8RP6gyS 36, 35 -- PorQmn1Ji2D4Fm5RQtP1jgnZa4vpBzKIyuXDc7VxWxDtuuiz9tFMR43XbYOg 36, 35 -- cgBsPgVqqVddb.bhOHDc52tAoc9.WtuamsMWq88VmzLrUfPzweGvuIu5NTEk 36, 35 -- PljgP1zF91z8bJ.8e4w.6.n4nI3s1pl8hU.fkgE.0IQzyk_P5JANFDxZ43XC 36, 35 -- OoOEdSKunatCgC9qq3Iny16Jx84f8SlLAQ7iXGBSz4eKg70aL40wdZpmIsU7 36, 35 -- 0HjZnjbYr71_j3pHhgHZLyt3q6JG7laCLME35PPXfHJywwTUaYm3IybbIkgK 36, 35 -- qI3SFb7RaVtVVgEBpudndAYBTKLUdHy6L_cCtBuq_CAN_4eCxSMQxMZRYht5 36, 35 -- 6fyHp5XIRcpKGMnGKkTWLn33uztDtvJiKoT9WLCXd8AZTZN_ks_3ZC6p5h9S 36, 35 -- cAadLphRLBA6VuTfKamfS1vtYzreK_AVAz0kCkXg- 36, 35 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 36, 35 -- From: Jan Leunissen {leunissen-at-aurion.nl} 36, 35 -- Content-Type: text/plain; charset=utf-8 36, 35 -- Subject: collected answers re "Epoxy Polymerisation at Lower Temperatures" 36, 35 -- Message-Id: {94883238-C0D4-44AF-A8C0-5B91E1BD73A4-at-aurion.nl} 36, 35 -- Date: Tue, 11 Aug 2015 08:21:26 +1200 36, 35 -- To: microscopy-at-microscopy.com 36, 35 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2102\)) 36, 35 -- X-Mailer: Apple Mail (2.2102) 36, 35 -- Content-Transfer-Encoding: 8bit 36, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7AKLTkO023971 ==============================End of - Headers==============================
I got an electron microscope a few months ago and just found this course that I'm going to start going through: https://www.coursera.org/learn/cryo-em/outline
Anyone else interested to work along with me?
My goal is to be able to repair my SEM and add in some custom instrumentation or mechanical stuff (sensors, gantry/stage type stuff). These goals are outside the scope of this course, but I expect the course will only aide my general comfort with the subject area.
My background is in Biotechnology and Computer Science, with electronics as a longtime hobby.
This might be pretty basic stuff for most of the folks on this list... but I figured I'd pass it along!
-- -Nathan
-- -Nathan
==============================Original Headers============================== 9, 26 -- From nmz787-at-gmail.com Mon Aug 10 21:29:31 2015 9, 26 -- Received: from mail-wi0-f180.google.com (mail-wi0-f180.google.com [209.85.212.180]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7B2TVkc027685 9, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2015 21:29:31 -0500 9, 26 -- Received: by wicja10 with SMTP id ja10so58037562wic.1 9, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2015 19:29:30 -0700 (PDT) 9, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 26 -- d=gmail.com; s=20120113; 9, 26 -- h=mime-version:from:date:message-id:subject:to:content-type; 9, 26 -- bh=rZxofhUWKBnoPC3NoTPlNmAW4W5FM70aJYrAj90LRm0=; 9, 26 -- b=eiFwRMfV8TN9L8q6jHxKItg2MclRhO4fbAloiU6oJKAJ+glX5bOZ7++qHH/F+RtHjQ 9, 26 -- 6Myt9WMnh2PE6D3tyosrU4j9PkXww44a6WIwjKwN69MhWR3cE775rHmxlDp/1vw9kGXA 9, 26 -- FWRhvkOVDg35ihPAEu4hnAhMaFcY6VrKhVAW5fhJFSA1fHYOUFoyfQXktW5YCb8nXLUF 9, 26 -- ML8ofyJg+dyaZcc9Ntj4q+4YgAfPN5/6OF3eqpeCO4g5S1zNA8j7tgKQniEDRzbFkqL4 9, 26 -- LCHCVv7xLCwgIA1NFYMAP5gzaPzLnQCSZFDFAj+Uv+uAEDd+U04PMVHRX5JZuySBHZyC 9, 26 -- wAnw== 9, 26 -- X-Received: by 10.180.205.148 with SMTP id lg20mr31772272wic.60.1439260170291; 9, 26 -- Mon, 10 Aug 2015 19:29:30 -0700 (PDT) 9, 26 -- MIME-Version: 1.0 9, 26 -- Received: by 10.28.217.205 with HTTP; Mon, 10 Aug 2015 19:29:10 -0700 (PDT) 9, 26 -- From: Nathan McCorkle {nmz787-at-gmail.com} 9, 26 -- Date: Mon, 10 Aug 2015 19:29:10 -0700 9, 26 -- Message-ID: {CA+82U9JnbWxi854m4t1iBgYvD8ZqN7kY+_ymYq_UXitTWjsxXw-at-mail.gmail.com} 9, 26 -- Subject: I'm taking a CRYO-EM class on Coursera! 9, 26 -- To: Microscopy-at-microscopy.com 9, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Hi Warren, I've got a JEOL JSM-T200, and a bunch of schematics and all the manuals as far as I can tell. I was told it works mostly, but that the image seemed like a beam-deflection problem was occurring. I haven't taken to looking into the problem much yet since I haven't really had the time lately, but I do have a nice hefty textbook and now found this course which I've been watching for an hour or so now. So far it's pretty good!
Hopefully I can get it working again without too much effort, but I'm up for trying to build a new beam deflection controller and deflection amplifiers! I've seen at least the controllers for sale for after-market patterning system upgrades. I've just got to get up to speed on power supplies and amplifiers for high-voltage before I can really make much progress on that. If I can trace a bad series of components with my multimeter and/or oscilloscope, all the better to get it up and running again sooner. But I'd LOVE to do patterning! I don't really want to think about much more than beam deflection and digital image acquisition for now though. At least someone else online has done digital acquisition with an oscilloscope and some matlab-type scripts (Ben Krasnow) with this same model... and I've been told by a guy who was previously a maintenance tech that these scopes were great to work on. He did say to watch out for some plastic supports that are prone to breaking in the lens coils, he said they can crack when (if) you need to remove to coils to remove hydrocarbon buildup.
Anyway, I didn't sink much into getting it, and it makes me giddy to be able to say I own one. It will be fun no matter what the outcome, which will at least be a mean set of pictures for takeitapart.com (which I am a co-creator of).
Thanks!
On Mon, Aug 10, 2015 at 7:35 PM, Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu} wrote: } It might help to know about which SEM you have and what condition it is in. } } I think SEMs are a blast. I've been working with them for almost 40 years. } } However, they can also be rather challenging to repair. I have debugged ours often but usually left the service to the factory engineers. Depending on what you are faced with, it could be a daunting task. } } Best of luck, } Warren } } -----Original Message----- } From: nmz787-at-gmail.com [mailto:nmz787-at-gmail.com] } Sent: Monday, August 10, 2015 9:31 PM } To: Straszheim, Warren E [BIOTC] } Subject: [Microscopy] I'm taking a CRYO-EM class on Coursera! } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I got an electron microscope a few months ago and just found this course that I'm going to start going through: } https://www.coursera.org/learn/cryo-em/outline } } Anyone else interested to work along with me? } } My goal is to be able to repair my SEM and add in some custom instrumentation or mechanical stuff (sensors, gantry/stage type stuff). These goals are outside the scope of this course, but I expect the course will only aide my general comfort with the subject area. } } My background is in Biotechnology and Computer Science, with electronics as a longtime hobby. } } This might be pretty basic stuff for most of the folks on this list... } but I figured I'd pass it along! } } } -- } -Nathan } } } -- } -Nathan } } ==============================Original Headers============================== } 9, 26 -- From nmz787-at-gmail.com Mon Aug 10 21:29:31 2015 9, 26 -- Received: from mail-wi0-f180.google.com (mail-wi0-f180.google.com [209.85.212.180]) } 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7B2TVkc027685 } 9, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2015 21:29:31 -0500 } 9, 26 -- Received: by wicja10 with SMTP id ja10so58037562wic.1 } 9, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2015 19:29:30 -0700 (PDT) } 9, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 26 -- d=gmail.com; s=20120113; } 9, 26 -- h=mime-version:from:date:message-id:subject:to:content-type; } 9, 26 -- bh=rZxofhUWKBnoPC3NoTPlNmAW4W5FM70aJYrAj90LRm0=; } 9, 26 -- b=eiFwRMfV8TN9L8q6jHxKItg2MclRhO4fbAloiU6oJKAJ+glX5bOZ7++qHH/F+RtHjQ } 9, 26 -- 6Myt9WMnh2PE6D3tyosrU4j9PkXww44a6WIwjKwN69MhWR3cE775rHmxlDp/1vw9kGXA } 9, 26 -- FWRhvkOVDg35ihPAEu4hnAhMaFcY6VrKhVAW5fhJFSA1fHYOUFoyfQXktW5YCb8nXLUF } 9, 26 -- ML8ofyJg+dyaZcc9Ntj4q+4YgAfPN5/6OF3eqpeCO4g5S1zNA8j7tgKQniEDRzbFkqL4 } 9, 26 -- LCHCVv7xLCwgIA1NFYMAP5gzaPzLnQCSZFDFAj+Uv+uAEDd+U04PMVHRX5JZuySBHZyC } 9, 26 -- wAnw== } 9, 26 -- X-Received: by 10.180.205.148 with SMTP id lg20mr31772272wic.60.1439260170291; } 9, 26 -- Mon, 10 Aug 2015 19:29:30 -0700 (PDT) 9, 26 -- MIME-Version: 1.0 9, 26 -- Received: by 10.28.217.205 with HTTP; Mon, 10 Aug 2015 19:29:10 -0700 (PDT) 9, 26 -- From: Nathan McCorkle {nmz787-at-gmail.com} 9, 26 -- Date: Mon, 10 Aug 2015 19:29:10 -0700 9, 26 -- Message-ID: {CA+82U9JnbWxi854m4t1iBgYvD8ZqN7kY+_ymYq_UXitTWjsxXw-at-mail.gmail.com} } 9, 26 -- Subject: I'm taking a CRYO-EM class on Coursera! } 9, 26 -- To: Microscopy-at-microscopy.com } 9, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
-- -Nathan
==============================Original Headers============================== 9, 31 -- From nmz787-at-gmail.com Mon Aug 10 23:18:51 2015 9, 31 -- Received: from mail-wi0-f181.google.com (mail-wi0-f181.google.com [209.85.212.181]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7B4IpOH016999 9, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2015 23:18:51 -0500 9, 31 -- Received: by wibhh20 with SMTP id hh20so177077562wib.0 9, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Aug 2015 21:18:50 -0700 (PDT) 9, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 31 -- d=gmail.com; s=20120113; 9, 31 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to 9, 31 -- :content-type:content-transfer-encoding; 9, 31 -- bh=xtwhAKrxIfdhZx07mSPGrjqZ1w7snzGAA3hh6/3YPMc=; 9, 31 -- b=T0q8mWjkVeU9vB1K/vLrCyKREJMe35oZK4FwQn+q5+38sqzscY1CNLrVchWAeQ51Yr 9, 31 -- spMOgy1t7vuBObdqnGH1dSkAuG1XXzqBOcWBo+biuXyF18tFVrw9LTB/eqYDrDin1TTB 9, 31 -- xmBnh8qzxWoHa2FfXJMI9+9xoULsyJ4DwBmMWd3+IRB+23AbDmYxKryNfwrEpjKdMkXV 9, 31 -- vqMg1zYakVR9r8by9+4pi2/ca+/GhQHY1BBdDmUv/2OTvuIsg+wm8/27jSNT0E/l2KjO 9, 31 -- Jdx124MTEP7cCZ5fEAh/puTNFwA1I+TnZ4Lz3aEO7F55GlQkaYLO4E6SD9mgOomvy7mz 9, 31 -- CB5g== 9, 31 -- X-Received: by 10.180.205.148 with SMTP id lg20mr32629213wic.60.1439266730445; 9, 31 -- Mon, 10 Aug 2015 21:18:50 -0700 (PDT) 9, 31 -- MIME-Version: 1.0 9, 31 -- Received: by 10.28.217.205 with HTTP; Mon, 10 Aug 2015 21:18:31 -0700 (PDT) 9, 31 -- In-Reply-To: {BN1PR04MB5529892547EFF35DE4F7EB4D77F0-at-BN1PR04MB552.namprd04.prod.outlook.com} 9, 31 -- References: {201508110231.t7B2VNGe028471-at-ns.microscopy.com} {BN1PR04MB5529892547EFF35DE4F7EB4D77F0-at-BN1PR04MB552.namprd04.prod.outlook.com} 9, 31 -- From: Nathan McCorkle {nmz787-at-gmail.com} 9, 31 -- Date: Mon, 10 Aug 2015 21:18:31 -0700 9, 31 -- Message-ID: {CA+82U9L_Rox8RAT8=4m-ZGT57Fjgf9ZTJuDHtK-x2tE5m_WgnQ-at-mail.gmail.com} 9, 31 -- Subject: Re: [Microscopy] I'm taking a CRYO-EM class on Coursera! 9, 31 -- To: Microscopy-at-microscopy.com 9, 31 -- Content-Type: text/plain; charset=UTF-8 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7B4IpOH016999 ==============================End of - Headers==============================
I'm being asked to run a rubber tank wall that may have small particles of a magnetic ore stuck to it's surface (monazite, I believe) in our SEM.
Some question has been raised that the magnetic particles could be pulled off the sticky rubber surface and end up in out column.
Has anyone any experience with this material and will it create a problem for us?
Thanks....... Frank Karl
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 7, 28 -- From frank_karl-at-ardl.com Tue Aug 11 13:48:38 2015 7, 28 -- Received: from cal1-mh677b.smtproutes.com (cal1-mh677b.smtproutes.com [208.70.89.140]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7BImbHE003819 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Aug 2015 13:48:37 -0500 7, 28 -- X-Katharion-ID: 1439318898.14959.cal1-mh677 7, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by 7, 28 -- cal1-mh677.smtproutes.com [(192.69.16.49)] with ESMTP via TCP 7, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 11 Aug 2015 18:48:18 +0000 7, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 7, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Tue, 11 7, 28 -- Aug 2015 14:48:18 -0400 7, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 7, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 7, 28 -- {microscopy-at-microscopy.com} 7, 28 -- Date: Tue, 11 Aug 2015 14:48:18 -0400 7, 28 -- Subject: SEM and mahnetic particles 7, 28 -- Thread-Topic: SEM and mahnetic particles 7, 28 -- Thread-Index: AdDUZkkMoBPJ5/H/QfGHeufA97V7uw== 7, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772CD8A4E2EE-at-exchange2k7.ad.ardl.com} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- acceptlanguage: en-US 7, 28 -- Content-Type: text/plain; charset="us-ascii" 7, 28 -- MIME-Version: 1.0 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7BImbHE003819 ==============================End of - Headers==============================
Has anyone written a plugin for ImageJ/Fiji that will calculate the perpendicular distance between an inner and outer line at various positions?
We need to measure the wall thickness on isolated plant cells which, some of which look like cross-sections of a cylinder. Now, if they were all nearly circular cylinders with even wall thickness, that'd be easy. But these cells are far from perfectly cylindrical in shape and the wall thickness is uneven.
In the past, we just made 4 measurements of the wall at fixed N-S-E-W positions and calculated the average thickness. But with the imaging tools available today, it should be possible to write a plugin that will do something like this - run a ball along the wall which shrinks and swells according to the wall thickness and record the ball diameter at every position, for example. That would not only give us the average thickness but some useful stats about variability (per cell and per sample) as well.
If anyone knows of something that will do this, you will have our eternal gratitude!
thanks, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 E rosemary.white-at-csiro.au
==============================Original Headers============================== 10, 45 -- From prvs=659a6e3bc=Rosemary.White-at-csiro.au Wed Aug 12 00:12:14 2015 10, 45 -- Received: from act-MTAout3.csiro.au (act-MTAout3.csiro.au [150.229.7.39]) 10, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7C5CDtC006152 10, 45 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Aug 2015 00:12:13 -0500 10, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 10, 45 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; 10, 45 -- t=1439356334; x=1470892334; 10, 45 -- h=from:to:subject:date:message-id:content-id: 10, 45 -- content-transfer-encoding:mime-version; 10, 45 -- bh=ZGQozuF/oy8xXA7TDVcgeXbj5EQnr/NudO3oxi6PFxM=; 10, 45 -- b=dfl+o9f+7EmB65ezUcUsYavst8XtmGDE6j8oJ6Ponp5isdqhPElhokmm 10, 45 -- IPTJGim2ziObwWhRjlHO+GYWoP/HC0Obz6SdMKc/Xd4v+GmbI0ryd6ay7 10, 45 -- GU4oeaCPBDKfihE; 10, 45 -- X-SBRS: 4.0 10, 45 -- X-IronPort-Anti-Spam-Filtered: true 10, 45 -- X-IronPort-Anti-Spam-Result: A+FaDwB81MpVjACwBSSwhIATAJKcgCRdgm+BaQaRO5s1lzCDDjwQAQEBAQEBAQMOAQEBJ0+EKjpRAT5CHwgEE4guAalXpi+QeYQVBZUSjGuaDoF9gidxgUiBBAEBAQ 10, 45 -- X-IPAS-Result: A+FaDwB81MpVjACwBSSwhIATAJKcgCRdgm+BaQaRO5s1lzCDDjwQAQEBAQEBAQMOAQEBJ0+EKjpRAT5CHwgEE4guAalXpi+QeYQVBZUSjGuaDoF9gidxgUiBBAEBAQ 10, 45 -- X-IronPort-AV: E=Sophos;i="5.15,658,1432562400"; 10, 45 -- d="scan'208";a="70070172" 10, 45 -- Received: from exhtca04-cdc.nexus.csiro.au ([IPv6:2405:b000:601:13::247:24]) 10, 45 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES256-SHA; 12 Aug 2015 15:12:10 +1000 10, 45 -- Received: from EXHTCA05-CDC.nexus.csiro.au (2405:b000:601:13::247:25) by 10, 45 -- exhtca04-cdc.nexus.csiro.au (2405:b000:601:13::247:24) with Microsoft SMTP 10, 45 -- Server (TLS) id 14.3.224.2; Wed, 12 Aug 2015 15:12:11 +1000 10, 45 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80::9de3:d7af:4ed1:b7b6]) by 10, 45 -- exhtca05-cdc.nexus.csiro.au ([fe80::fde4:64ec:c03d:871%13]) with mapi id 10, 45 -- 14.03.0224.002; Wed, 12 Aug 2015 15:12:11 +1000 10, 45 -- From: {Rosemary.White-at-csiro.au} 10, 45 -- To: {Microscopy-at-microscopy.com} 10, 45 -- Subject: measuring wall thickness 10, 45 -- Thread-Topic: measuring wall thickness 10, 45 -- Thread-Index: AQHQ1L1wYxAc1GxJYkK9K7PzDv2EeQ== 10, 45 -- Date: Wed, 12 Aug 2015 05:12:09 +0000 10, 45 -- Message-ID: {D1F112C8.1A3E90%rosemary.white-at-csiro.au} 10, 45 -- Accept-Language: en-NZ, en-US, en-AU 10, 45 -- Content-Language: en-US 10, 45 -- X-MS-Has-Attach: 10, 45 -- X-MS-TNEF-Correlator: 10, 45 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 10, 45 -- x-originating-ip: [152.83.195.117] 10, 45 -- Content-Type: text/plain; charset="us-ascii" 10, 45 -- Content-ID: {B4D886B824F19E42AA1A75357227C89B-at-csiro.au} 10, 45 -- MIME-Version: 1.0 10, 45 -- Content-Transfer-Encoding: 8bit 10, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7C5CDtC006152 ==============================End of - Headers==============================
} On Aug 11, 2015, at 10:27 PM, Rosemary.White-at-csiro.au wrote: } } Apologies for cross-posting from confocal list. } } Has anyone written a plugin for ImageJ/Fiji that will calculate the } perpendicular distance between an inner and outer line at various } positions? } } We need to measure the wall thickness on isolated plant cells which, some } of which look like cross-sections of a cylinder. Now, if they were all } nearly circular cylinders with even wall thickness, that'd be easy. But } these cells are far from perfectly cylindrical in shape and the wall } thickness is uneven. } } In the past, we just made 4 measurements of the wall at fixed N-S-E-W } positions and calculated the average thickness. But with the imaging tools } available today, it should be possible to write a plugin that will do } something like this - run a ball along the wall which shrinks and swells } according to the wall thickness and record the ball diameter at every } position, for example. That would not only give us the average thickness } but some useful stats about variability (per cell and per sample) as well. } } If anyone knows of something that will do this, you will have our eternal } gratitude! } } thanks, } Rosemary } } Dr Rosemary White } CSIRO Black Mountain } GPO Box 1600 } Canberra, ACT 2601 } Australia
Dear Rosemary, The 2D filament plug-in might do the job, although more will have to be added. With this plug-in, you can make âsnakesâ that follow edges, so you could find the inside and outside edges of cross-sections of the cells. Higher contrast works better, so using this with cryo data can be problematical. After you have traced the edges, you are still left with the task of determining the distance between them, but maybe someone else on either list can help. Yours, Bill
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From advertise.bz222zyjle-at-gmail.com Thu Aug 13 05:15:23 2015 Return-Path: {advertise.bz222zyjle-at-gmail.com} Received: from gmail.com ([121.254.237.37]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t7DAFKhI009472 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 13 Aug 2015 05:15:22 -0500 Message-ID: {9C57FDC4.95BBF015-at-gmail.com}
One of our Wild M5 stereomicroscopes, serial number 32035, with transmitted light base and transport hood, has been stolen from the Biology Building. This is the old beige model with the large knobs. Reward of $100 for information leading to its return. Thanks for any leads, and apologies for clogging the inboxes of those of you outside of the U.S. Julian
-- Julian P.S. Smith III Director, Winthrop Microscopy Facility Dept. of Biology Winthrop University 349 Columbia Ave Rock Hill, SC 29733
803-323-2111 x6427 (vox) 803-323-3448 (fax) 803-524-2347 (cell) Research Website www.birdnest.org/smithj Personal Website www.rociada-east.net
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I have several spinal root fibers from rats that were fixed in 4% paraformaldehyde and 1% glutaraldehyde in phosphate buffer, followed by 1% osmium in NaCacodylate, then dehydrated and embedded in an Epon-812 replacement epoxy. After cutting semi-thins (400 nm), the sections were stained on a hot plate with 1% Toluidine Blue O in 1% Na Borate. This usually results in uniform staining of the root fibers, but this particular batch of fibers stained unevenly. The uneven pattern is usually a gradient with the outer edge stained light blue, and the inner portion stained dark blue. Sometimes the center fades back to light blue. The axons look good throughout and are not disrupted (at least, not at the light microscope level). Can anyone tell me why I am getting this pattern, and is there anything I can do to get uniform staining on these samples?
Thank you,
Sharon W. Matthews, Ph.D. Senior Electron Microscopist COM Electron Microscopy Core University of Florida Room RB-167, 1600 SW Archer Road PO Box 100224 HSC Gainesville, FL 32610 Telephone: (352) 846-0821 Fax: (352) 392-8996 Email: emcore-at-medicine.ufl.edu
==============================Original Headers============================== 6, 48 -- From Sharon.Matthews-at-medicine.ufl.edu Thu Aug 13 16:16:19 2015 6, 48 -- Received: from smtp.ufl.edu (smtp-prod04.osg.ufl.edu [128.227.74.220]) 6, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7DLGJR8006707 6, 48 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Aug 2015 16:16:19 -0500 6, 48 -- X-UFL-GatorLink-Authenticated: authenticated as () with from 10.36.133.35 6, 48 -- Received: from exmbxprd04.ad.ufl.edu ([10.36.133.35]) 6, 48 -- by smtp.ufl.edu (8.14.4/8.14.4/3.0.0) with ESMTP id t7DLG8Zr001880 6, 48 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 6, 48 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Aug 2015 17:16:18 -0400 6, 48 -- Received: from AHC-EXCH03.ad.ufl.edu (10.15.93.86) by exmbxprd04.ad.ufl.edu 6, 48 -- (10.36.133.35) with Microsoft SMTP Server (TLS) id 15.0.1076.9; Thu, 13 Aug 6, 48 -- 2015 17:16:15 -0400 6, 48 -- Received: from AHC-EXCH08.ad.ufl.edu (10.15.93.96) by AHC-EXCH03.ad.ufl.edu 6, 48 -- (10.15.93.86) with Microsoft SMTP Server (TLS) id 15.0.1076.9; Thu, 13 Aug 6, 48 -- 2015 17:16:14 -0400 6, 48 -- Received: from AHC-EXCH08.ad.ufl.edu ([fe80::e9e9:53aa:98d3:7315]) by 6, 48 -- AHC-EXCH08.ad.ufl.edu ([fe80::e9e9:53aa:98d3:7315%18]) with mapi id 6, 48 -- 15.00.1076.000; Thu, 13 Aug 2015 17:16:14 -0400 6, 48 -- From: "Matthews,Sharon W" {Sharon.Matthews-at-medicine.ufl.edu} 6, 48 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 48 -- Subject: Uneven staining of epoxy semi-thin sections 6, 48 -- Thread-Topic: Uneven staining of epoxy semi-thin sections 6, 48 -- Thread-Index: AdDWCCx6X6fq1yobRZCZ1MS02B1Weg== 6, 48 -- Importance: high 6, 48 -- X-Priority: 1 6, 48 -- Date: Thu, 13 Aug 2015 21:16:14 +0000 6, 48 -- Message-ID: {9c88f83c9c2843f9884a042ad4c3102f-at-AHC-EXCH08.ad.ufl.edu} 6, 48 -- Accept-Language: en-US 6, 48 -- Content-Language: en-US 6, 48 -- X-MS-Has-Attach: 6, 48 -- X-MS-TNEF-Correlator: 6, 48 -- x-ms-exchange-transport-fromentityheader: Hosted 6, 48 -- x-originating-ip: [10.15.92.201] 6, 48 -- x-tm-as-product-ver: SMEX-11.0.0.4179-8.000.1202-21742.004 6, 48 -- x-tm-as-result: No--37.977800-8.000000-31 6, 48 -- x-tm-as-user-approved-sender: No 6, 48 -- x-tm-as-user-blocked-sender: No 6, 48 -- Content-Type: text/plain; charset="iso-8859-1" 6, 48 -- MIME-Version: 1.0 6, 48 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.14.151,1.0.33,0.0.0000 6, 48 -- definitions=2015-08-13_10:2015-08-13,2015-08-13,1970-01-01 signatures=0 6, 48 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 6, 48 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 6, 48 -- engine=7.0.1-1508030000 definitions=main-1508130323 6, 48 -- X-Spam-Level: * 6, 48 -- X-UFL-Spam-Level: * 6, 48 -- Content-Transfer-Encoding: 8bit 6, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7DLGJR8006707 ==============================End of - Headers==============================
I also registerd for this course, even if I doubt I can do cryo-EM with the old tungsten filament electron microscope I have, but I hope to be albe to apply some of the things I will learn in this course. I use the SEM in my lab but was never formally trained, so nothing is too basic for me.
Virginia Soares INESC-MN (Microsystems and Nanotechnologies) R. Alves Redol, 9 1000-029 LISBON Tel: +351213100300 Fax: +351213145843 www.inesc-mn.pt www.in-nano.net ________________________________________ De: nmz787-at-gmail.com [nmz787-at-gmail.com] Enviado: terça-feira, 11 de Agosto de 2015 3:50 Para: Virginia Abranches de Sousa R. Soares Assunto: [Microscopy] I'm taking a CRYO-EM class on Coursera!
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I got an electron microscope a few months ago and just found this course that I'm going to start going through: https://www.coursera.org/learn/cryo-em/outline
Anyone else interested to work along with me?
My goal is to be able to repair my SEM and add in some custom instrumentation or mechanical stuff (sensors, gantry/stage type stuff). These goals are outside the scope of this course, but I expect the course will only aide my general comfort with the subject area.
My background is in Biotechnology and Computer Science, with electronics as a longtime hobby.
This might be pretty basic stuff for most of the folks on this list... but I figured I'd pass it along!
-- -Nathan
-- -Nathan
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==============================Original Headers============================== 14, 32 -- From vsoares-at-inesc-mn.pt Fri Aug 14 06:04:30 2015 14, 32 -- Received: from smtp.inesc.pt (smtp.inesc.pt [146.193.0.2]) 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7EB4SZN000663 14, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Aug 2015 06:04:29 -0500 14, 32 -- Received: from PsExFront.inesc-mn.pt (PsExFront.inesc-mn.pt [146.193.96.251]) 14, 32 -- by smtp.inesc.pt (8.13.1/8.13.1/SuSE Linux 0.7) with ESMTP id t7EB4PVm002106; 14, 32 -- Fri, 14 Aug 2015 12:04:26 +0100 14, 32 -- Received: from PsExFront.inesc-mn.pt ([2002:92c1:60fb::92c1:60fb]) by 14, 32 -- PsExFront.inesc-mn.pt ([::1]) with mapi; Fri, 14 Aug 2015 12:02:52 +0100 14, 32 -- From: "Virginia Abranches de Sousa R. Soares" {vsoares-at-inesc-mn.pt} 14, 32 -- To: "nmz787-at-gmail.com" {nmz787-at-gmail.com} , 14, 32 -- "Microscopy-at-microscopy.com" 14, 32 -- {Microscopy-at-microscopy.com} 14, 32 -- Date: Fri, 14 Aug 2015 12:02:51 +0100 14, 32 -- Subject: RE: [Microscopy] I'm taking a CRYO-EM class on Coursera! 14, 32 -- Thread-Topic: [Microscopy] I'm taking a CRYO-EM class on Coursera! 14, 32 -- Thread-Index: AdDT4FBvm1dBSvO/RwaarLPJRr4L6QCn6WJ6 14, 32 -- Message-ID: {F98E23EE61DD534E8C2FF73AFFB28D7902FBF6E79334-at-PsExFront.inesc-mn.pt} 14, 32 -- References: {201508110250.t7B2oS67006048-at-ns.microscopy.com} 14, 32 -- In-Reply-To: {201508110250.t7B2oS67006048-at-ns.microscopy.com} 14, 32 -- Accept-Language: pt-PT 14, 32 -- Content-Language: pt-PT 14, 32 -- X-MS-Has-Attach: 14, 32 -- X-MS-TNEF-Correlator: 14, 32 -- acceptlanguage: pt-PT 14, 32 -- x-kse-antivirus-interceptor-info: scan successful 14, 32 -- x-kse-antivirus-info: Clean 14, 32 -- Content-Type: text/plain; charset="iso-8859-1" 14, 32 -- MIME-Version: 1.0 14, 32 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.4.1 (smtp.inesc.pt [146.193.0.2]); Fri, 14 Aug 2015 12:04:27 +0100 (WEST) 14, 32 -- Content-Transfer-Encoding: 8bit 14, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7EB4SZN000663 ==============================End of - Headers==============================
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The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community. A major focus of the CRTP is the NCI RAS Initiative with the goal to discover new therapeutic interventions against RAS-driven cancers. In addition, the CRTP hosts the Nanotechnology Characterization Laboratory (NCL) which performs and standardizes preclinical efficacy and toxicity testing of nanoparticles intended for cancer therapeutics and diagnostics, and the Antibody Characterization Lab (ACL) which characterizes monoclonal antibodies or other renewable affinity binding reagents for use in cancer related research. CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.
JOB DESCRIPTION
The CCR Electron Microscopy Core has access to four transmission electron microscopes (Hitachi H-7600, Hitachi H-7650, FEI T-12 BioTwin, and FEI T-20) and two scanning electron microscopes (Hitachi S-3000N and S-4500). The Scientist I will: 1) maintain the FEI T-12 and keep it at optimal performance, 2) perform sample preparation for imaging of tissue, cells, organelles, viruses and molecules with a variety of techniques including plastic embedding and sectioning, high-pressure freezing, negative stain, and electron tomography, and 3) help core users with their microscopy projects and perform training of new microscope users.
BASIC QUALIFICATIONS PhD in biochemistry, structural biology, protein biochemistry, crystallography, or in a related discipline from an accredited college or university appropriate to biomedical research, or eight (8) years experience in lieu of degree Foreign degrees must be evaluated for U.S. Equivalency No experience required beyond degree Proficiency in biological and/or material sample preparation for electron microscopy Proficiency in the use and maintenance of transmission electron microscopes Strong research background and publication record Must be able to communicate well with service engineers for equipment on service contracts
PREFERRED QUALIFICATIONS Experience in correlative light/electron microscopy Experience in immuno-electron microscopy Knowledge of tomography and related image processing Experience with cryo EM applications, in particular high pressure freezing, freeze substitution, and cryo-sectioning Knowledge of digital image processing and analysis
Leidos Overview: Leidos is an applied solutions company focused on markets that are seeing converging business and technological trends, and address basic, enduring human needs: defense and national security, health and life sciences, and energy, engineering and infrastructure. The Company's approximately 20,000 employees serve customers in the U.S. Department of Defense, the intelligence community, the U.S. Department of Homeland Security, other U.S. Government civil agencies and commercial health and engineering markets. Leidos is an Equal Opportunity Employer.
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==============================Original Headers============================== 28, 40 -- From zaluzec.microscopy.com-at-gmail.com Fri Aug 14 07:43:54 2015 28, 40 -- Received: from mail-ig0-f170.google.com (mail-ig0-f170.google.com [209.85.213.170]) 28, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7EChsHv026107 28, 40 -- for {microscopy-at-microscopy.com} ; Fri, 14 Aug 2015 07:43:54 -0500 28, 40 -- Received: by igbjg10 with SMTP id jg10so11045570igb.0 28, 40 -- for {microscopy-at-microscopy.com} ; Fri, 14 Aug 2015 05:43:54 -0700 (PDT) 28, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 28, 40 -- d=gmail.com; s=20120113; 28, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 28, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 28, 40 -- bh=Z0HObUKZV38SLMxf6hLHzZ4tNaguIG1gmMUtKfQX+0Y=; 28, 40 -- b=KHC+eCXF8MJG/Q47TYY+sS5gdQk1eHij3+JD62+9aWZbaJfs20dIkpadqqSdbkkRMN 28, 40 -- GegWrkXjh0ku/0RXWQMmVIf05iuoSvGCtbKfm9Oj04KL7tgzwjIkw15JPhuc06CpCCxR 28, 40 -- AG9E46hbAGRC5wQQjArtLik9p00v/lvp+nvoXSEiLvDg1bJiRAT8rDyutr05OwvAhOGH 28, 40 -- heGQh32X1HjFibn0ULpaMaBkwnk7KtiyGiJ1JZ7KNPRi+EMhCxNKUKY3ncduibMl8GHL 28, 40 -- Nme4BZQ80EhF0oFO4+YH9WYZACsixlgKkCwyAD5S0XVlo8ftXhG2QXeHnOV59Ewkay0U 28, 40 -- nIUg== 28, 40 -- X-Received: by 10.50.61.209 with SMTP id s17mr2279537igr.51.1439556233999; 28, 40 -- Fri, 14 Aug 2015 05:43:53 -0700 (PDT) 28, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 28, 40 -- by smtp.googlemail.com with ESMTPSA id 88sm4639082ioj.25.2015.08.14.05.43.52 28, 40 -- for {microscopy-at-microscopy.com} 28, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 28, 40 -- Fri, 14 Aug 2015 05:43:53 -0700 (PDT) 28, 40 -- From: MicroscopyListserver-NoReply {zaluzec.microscopy.com-at-gmail.com} 28, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 28, 40 -- {microscopylistserver-noreply-at-microscopy.com} 28, 40 -- Subject: viaWWW:Position Open - Scientist I, Electron Microscopy, 618013 28, 40 -- References: {201508131939.t7DJdbPH001685-at-ns.microscopy.com} 28, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 28, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 28, 40 -- X-Forwarded-Message-Id: {201508131939.t7DJdbPH001685-at-ns.microscopy.com} 28, 40 -- Message-ID: {55CDE2A5.6070200-at-microscopy.com} 28, 40 -- Date: Fri, 14 Aug 2015 07:44:21 -0500 28, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 28, 40 -- Gecko/20100101 Thunderbird/38.1.0 28, 40 -- MIME-Version: 1.0 28, 40 -- In-Reply-To: {201508131939.t7DJdbPH001685-at-ns.microscopy.com} 28, 40 -- Content-Type: text/plain; charset=UTF-8; format=flowed 28, 40 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From advertise.bz222yg-at-gmail.com Fri Aug 14 12:26:31 2015 Return-Path: {advertise.bz222yg-at-gmail.com} Received: from gmail.com ([220.241.216.221]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t7EHQS1c020875 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 14 Aug 2015 12:26:30 -0500 Message-ID: {1D12CACC.E39D21E8-at-gmail.com}
Trying to locate a local lab with these analytical techniques.
BET (Brunauer-Emmett-Teller) provides precise specific surface area in m2/g and pore area evaluation of materials by nitrogen multilayer adsorption. It is a very commonly used equipment in many labs.
DLS (Dynamic Light Scattering) measures particle sizes. We have been trying to understand the particle size of nano sized colloids in solution. The SEM images show that the particles get swelled and/or clustered when we place them in water, and so it became very difficult to understand the exact size when we have them in solution. So DLS will be extremely helpful for us to understand the size of particle aggregates.
Contact me directly if you can help.
Thanks
Roy Beavers Southern Methodist University rbeavers-at-smu.edu
==============================Original Headers============================== 5, 31 -- From rbeavers-at-mail.smu.edu Fri Aug 14 16:45:40 2015 5, 31 -- Received: from smtap1.systems.smu.edu (smtap1.systems.smu.edu [129.119.65.148]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7ELjePr029679 5, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Aug 2015 16:45:40 -0500 5, 31 -- X-IronPort-Anti-Spam-Filtered: true 5, 31 -- X-IronPort-Anti-Spam-Result: A2H+BAAAYM5V/4ZBd4Fdgm8sgT0GxXoCgUw8EAEBAQEBAQEDgQeEJQWBCwEqViYBBBMIE4gTAalboQqFO5Arg1CBFAWNFIgHAW6mISaDfXGBSIEEAQEB 5, 31 -- X-IPAS-Result: A2H+BAAAYM5V/4ZBd4Fdgm8sgT0GxXoCgUw8EAEBAQEBAQEDgQeEJQWBCwEqViYBBBMIE4gTAalboQqFO5Arg1CBFAWNFIgHAW6mISaDfXGBSIEEAQEB 5, 31 -- X-IronPort-AV: E=McAfee;i="5700,7163,7893"; a="158403" 5, 31 -- X-IronPort-AV: E=Sophos;i="5.15,680,1432616400"; 5, 31 -- d="scan'208";a="158403" 5, 31 -- Received: from sxht1p3.systems.smu.edu ([129.119.65.134]) 5, 31 -- by smtah1.systems.smu.edu with ESMTP/TLS/AES128-SHA; 14 Aug 2015 16:45:39 -0500 5, 31 -- Received: from SXMB1PG.SYSTEMS.SMU.EDU ([fe80::1449:c186:5adb:ddbb]) by 5, 31 -- SXHT1P3.SYSTEMS.SMU.EDU ([fe80::4479:3bff:ca05:ebce%17]) with mapi id 5, 31 -- 14.03.0224.002; Fri, 14 Aug 2015 16:45:39 -0500 5, 31 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 5, 31 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 31 -- Subject: Labs in the Dallas Tx area for these analysis techniques 5, 31 -- Thread-Topic: Labs in the Dallas Tx area for these analysis techniques 5, 31 -- Thread-Index: AdDW2hzD69HUE9r3Rci9cErTFPLIwQ== 5, 31 -- Date: Fri, 14 Aug 2015 21:45:38 +0000 5, 31 -- Message-ID: {BC0C36E74D341741A25CE2ABB122E7A350C21505-at-SXMB1PG.SYSTEMS.SMU.EDU} 5, 31 -- Accept-Language: en-US 5, 31 -- Content-Language: en-US 5, 31 -- X-MS-Has-Attach: 5, 31 -- X-MS-TNEF-Correlator: 5, 31 -- x-originating-ip: [129.119.65.139] 5, 31 -- Content-Type: text/plain; charset="iso-8859-1" 5, 31 -- MIME-Version: 1.0 5, 31 -- Content-Transfer-Encoding: 8bit 5, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7ELjePr029679 ==============================End of - Headers==============================
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Email: kavanagh-at-sfu.ca Name: Karen Kavanagh
Organization: Simon Fraser University
Title-Subject: [Filtered] New Postion Open: Helium Ion Microscopist
Establish a new Heilum Ion Microscopy facility within a larger microscopy user facility. Establish research and development programs using the instrument. Facilitate access by other researchers.
PREFERRED QUALIFICATIONS:
PhD in one of (MSE, Physics, Appl. Physics, Chemistry, Biology, etc.) Minimum of 4 years experience using a HIM. Proficiency with other types of microscopy including SEM, Ne or Ga FIB, and or STEM, an asset. Record of publications with portfolio of examples of microscopy skills that include HIM.
See further details at: www.4dlabs.ca/about/job-postings
Send your CV to info-at-4dlabs.ca Questions please ask Karen Kavanagh: kavanagh-at-sfu.ca
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] SEM imaging of starch grains
Message: Greetings,
I have a user from our Food Sciences Dept with various starch specimens in need of SEM imaging. Initial attempts were made with no specific prep other than carbon coating [using an evaporative rod coater]. Results were okay but not great. Grains were often fractured and would rupture under the beam after approx 30 seconds of exposure [used a 5 kV AV under normal High Vac conditions].
This geologist humbly requests input or suggested sample prep procedures for starch [such as metal sputter coating vs carbon coating, or drying/dessication??].
My experience with starch is eating a donut.....
Any assistance would be greatly appreciated!
Cheers, Tom Williams
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==============================Original Headers============================== 17, 40 -- From zaluzec.microscopy.com-at-gmail.com Sat Aug 15 08:33:35 2015 17, 40 -- Received: from mail-ig0-f182.google.com (mail-ig0-f182.google.com [209.85.213.182]) 17, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7FDXZJk013476 17, 40 -- for {microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 08:33:35 -0500 17, 40 -- Received: by igui7 with SMTP id i7so28548818igu.0 17, 40 -- for {microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 06:33:34 -0700 (PDT) 17, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 40 -- d=gmail.com; s=20120113; 17, 40 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 17, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 17, 40 -- bh=gYm0QSF+VWJADHcQhf5+riKaabe2PRtJHCCbL8ULcho=; 17, 40 -- b=FkAIKr3lwF6vXvewTSxviTyPieAfDMxEy19r8hy0s8NmpZZEGusxIniCtLGSCJ5l55 17, 40 -- kWMFgPH36m2RVXC9NNbckdTW4f9So4wXEfKxP9tIRNh329sOpp+s9khu9+dahQD7OgN/ 17, 40 -- nuptBfDxS+EsCDj6nb8ph7aY9HGT9Dn7bMricHqP/JG8lNDDF2CSoOS50KX1jf3/9kBF 17, 40 -- 5vgClgGN8b6FJtiqcMntrFC1buGoymSUTh/s7w8xsTMSmlCw/d7rUCG+QsJm3Sjr9GnI 17, 40 -- Yiuk8yrvr84y3szMERQz/cvN/7NOTwnV2zSUF/cp5AeDts0t3YC7TEz+EKkw7cDFzzAJ 17, 40 -- 8PPg== 17, 40 -- X-Received: by 10.50.136.134 with SMTP id qa6mr7478215igb.13.1439645614598; 17, 40 -- Sat, 15 Aug 2015 06:33:34 -0700 (PDT) 17, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 17, 40 -- by smtp.googlemail.com with ESMTPSA id i13sm7831418ioe.4.2015.08.15.06.33.33 17, 40 -- for {microscopy-at-microscopy.com} 17, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 17, 40 -- Sat, 15 Aug 2015 06:33:33 -0700 (PDT) 17, 40 -- From: MicroscopyListserver-NoReply {zaluzec.microscopy.com-at-gmail.com} 17, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 17, 40 -- {microscopylistserver-noreply-at-microscopy.com} 17, 40 -- Subject: viaWWW:SEM imaging of starch grains 17, 40 -- References: {201508141659.t7EGxwht020225-at-ns.microscopy.com} 17, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 40 -- X-Forwarded-Message-Id: {201508141659.t7EGxwht020225-at-ns.microscopy.com} 17, 40 -- Message-ID: {55CF3FAC.4060000-at-microscopy.com} 17, 40 -- Date: Sat, 15 Aug 2015 08:33:32 -0500 17, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 17, 40 -- Gecko/20100101 Thunderbird/38.1.0 17, 40 -- MIME-Version: 1.0 17, 40 -- In-Reply-To: {201508141659.t7EGxwht020225-at-ns.microscopy.com} 17, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: lavoie-at-uw.edu Name: Ellen Lavoie
Organization: Univ Washington
Title-Subject: [Filtered] SF6 gas in TEM rooms
Message: Curious to know how others are handling SF6 gas detection in their TEM labs right now.
Is your room sealed well from draft and/or air flow? If so (or even if not) do you have an SF6 and/or O2 monitor in the room?
Happy to have either public or private replies.
Cheers, Ellen Lavoie UW - Material Analysis Facility Seattle, WA, USA
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==============================Original Headers============================== 14, 30 -- From microscopylistserver-noreply-at-microscopy.com Sat Aug 15 08:38:23 2015 14, 30 -- Received: from znl.com ([206.69.208.20]) 14, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7FDcNHc017829 14, 30 -- for {microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 08:38:23 -0500 14, 30 -- Received: from localhost (localhost [127.0.0.1]) 14, 30 -- by znl.com (Postfix) with ESMTP id 3BB538EB7F3 14, 30 -- for {microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 08:38:23 -0500 (CDT) 14, 30 -- X-Virus-Scanned: amavisd-new at znl.com 14, 30 -- Received: from znl.com ([127.0.0.1]) 14, 30 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 14, 30 -- with ESMTP id pKHzhVIL_Oqn for {microscopy-at-microscopy.com} ; 14, 30 -- Sat, 15 Aug 2015 08:38:22 -0500 (CDT) 14, 30 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 14, 30 -- by znl.com (Postfix) with ESMTPA id AC6538EB7E6 14, 30 -- for {microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 08:38:22 -0500 (CDT) 14, 30 -- Subject: viaWWW:SF6 gas in TEM rooms 14, 30 -- References: {201508142209.t7EM9kkL017798-at-ns.microscopy.com} 14, 30 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 30 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 30 -- From: MicroscopyListserver-NoReply 14, 30 -- {microscopylistserver-noreply-at-microscopy.com} 14, 30 -- X-Forwarded-Message-Id: {201508142209.t7EM9kkL017798-at-ns.microscopy.com} 14, 30 -- Message-ID: {55CF40C8.8050100-at-microscopy.com} 14, 30 -- Date: Sat, 15 Aug 2015 08:38:16 -0500 14, 30 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 30 -- Gecko/20100101 Thunderbird/38.1.0 14, 30 -- MIME-Version: 1.0 14, 30 -- In-Reply-To: {201508142209.t7EM9kkL017798-at-ns.microscopy.com} 14, 30 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 30 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: lavoie-at-uw.edu Name: Ellen Lavoie
Organization: Univ Washington
Title-Subject: [Filtered] SF6 gas in TEM rooms
Message: Curious to know how others are handling SF6 gas detection in their TEM labs right now.
Is your room sealed well from draft and/or air flow? If so (or even if not) do you have an SF6 and/or O2 monitor in the room?
Happy to have either public or private replies.
Cheers, Ellen Lavoie UW - Material Analysis Facility Seattle, WA, USA
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Email: kpseverin-at-alaska.edu Name: Ken Severin
Organization: University of Alaska Fairbanks
Title-Subject: [Filtered] ISI-SR50 available
Message: We are (finally) taking our ISI-SR50 (tungsten) out of service. If someone in interested it it (or parts of it) please contact me. Shipping from Alaska could prove problematic, or at least expensive. The instrument was running until I pulled the plug.
Ken Severin kpseverin-at-alaska.edu University of Alaska Fairbanks
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Email: kpseverin-at-alaska.edu Name: Ken Severin
Organization: University of Alaska Fairbanks
Title-Subject: [Filtered] ISI-SR50 available
Message: We are (finally) taking our ISI-SR50 (tungsten) out of service. If someone in interested it it (or parts of it) please contact me. Shipping from Alaska could prove problematic, or at least expensive. The instrument was running until I pulled the plug.
Ken Severin kpseverin-at-alaska.edu University of Alaska Fairbanks
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A food scientist here is looking at gluten and gluten-free baked goods and dough, and is looking at starch grains from things like breadfruit flour. These are all "gushy" preps. What we've been doing is freezing pieces in liquid nitrogen (decided it wasn't worth trying to use a better cryogen for this), then throwing them down on the benchtop to "cryofracture" them, then freeze-drying them, since food scientists tend to have good freeze-dryers. Then mount on stubs, coat, and they've been pretty good. Of course, in this case, trying to decide what's a starch granule vs a fat glob has been fun, but these guys think they know. (I reserve jusdement.) If the material would not fracture by dropping it on the bench, we used the razor-bade-hammer-popitopen technique.
Aloha, Tina
} Message: Greetings, } } I have a user from our Food Sciences Dept with various starch specimens in need of SEM imaging. } Initial attempts were made with no specific prep other than carbon coating [using an evaporative rod } coater]. Results were okay but not great. Grains were often fractured and would rupture under the } beam after approx 30 seconds of exposure [used a 5 kV AV under normal High Vac conditions]. } } This geologist humbly requests input or suggested sample prep procedures for starch [such as metal } sputter coating vs carbon coating, or drying/dessication??]. } } My experience with starch is eating a donut..... } } Any assistance would be greatly appreciated! } } Cheers, } Tom Williams } } } Login Host: 129.101.55.31 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original Headers============================== } 17, 40 -- From zaluzec.microscopy.com-at-gmail.com Sat Aug 15 08:33:35 2015 } 17, 40 -- Received: from mail-ig0-f182.google.com (mail-ig0-f182.google.com [209.85.213.182]) } 17, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7FDXZJk013476 } 17, 40 -- for {microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 08:33:35 -0500 } 17, 40 -- Received: by igui7 with SMTP id i7so28548818igu.0 } 17, 40 -- for {microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 06:33:34 -0700 (PDT) } 17, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 17, 40 -- d=gmail.com; s=20120113; } 17, 40 -- h=from:subject:references:reply-to:to:message-id:date:user-agent } 17, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; } 17, 40 -- bh=gYm0QSF+VWJADHcQhf5+riKaabe2PRtJHCCbL8ULcho=; } 17, 40 -- b=FkAIKr3lwF6vXvewTSxviTyPieAfDMxEy19r8hy0s8NmpZZEGusxIniCtLGSCJ5l55 } 17, 40 -- kWMFgPH36m2RVXC9NNbckdTW4f9So4wXEfKxP9tIRNh329sOpp+s9khu9+dahQD7OgN/ } 17, 40 -- nuptBfDxS+EsCDj6nb8ph7aY9HGT9Dn7bMricHqP/JG8lNDDF2CSoOS50KX1jf3/9kBF } 17, 40 -- 5vgClgGN8b6FJtiqcMntrFC1buGoymSUTh/s7w8xsTMSmlCw/d7rUCG+QsJm3Sjr9GnI } 17, 40 -- Yiuk8yrvr84y3szMERQz/cvN/7NOTwnV2zSUF/cp5AeDts0t3YC7TEz+EKkw7cDFzzAJ } 17, 40 -- 8PPg== } 17, 40 -- X-Received: by 10.50.136.134 with SMTP id qa6mr7478215igb.13.1439645614598; } 17, 40 -- Sat, 15 Aug 2015 06:33:34 -0700 (PDT) } 17, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) } 17, 40 -- by smtp.googlemail.com with ESMTPSA id i13sm7831418ioe.4.2015.08.15.06.33.33 } 17, 40 -- for {microscopy-at-microscopy.com} } 17, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 17, 40 -- Sat, 15 Aug 2015 06:33:33 -0700 (PDT) } 17, 40 -- From: MicroscopyListserver-NoReply {zaluzec.microscopy.com-at-gmail.com} } 17, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply } 17, 40 -- {microscopylistserver-noreply-at-microscopy.com} } 17, 40 -- Subject: viaWWW:SEM imaging of starch grains } 17, 40 -- References: {201508141659.t7EGxwht020225-at-ns.microscopy.com} } 17, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 17, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 17, 40 -- X-Forwarded-Message-Id: {201508141659.t7EGxwht020225-at-ns.microscopy.com} } 17, 40 -- Message-ID: {55CF3FAC.4060000-at-microscopy.com} } 17, 40 -- Date: Sat, 15 Aug 2015 08:33:32 -0500 } 17, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) } 17, 40 -- Gecko/20100101 Thunderbird/38.1.0 } 17, 40 -- MIME-Version: 1.0 } 17, 40 -- In-Reply-To: {201508141659.t7EGxwht020225-at-ns.microscopy.com} } 17, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 17, 40 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 22 -- From tina-at-pbrc.hawaii.edu Sat Aug 15 14:02:58 2015 5, 22 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7FJ2vus028899 5, 22 -- for {Microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 14:02:57 -0500 5, 22 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 22 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id t7FJ2umB007290 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 22 -- Sat, 15 Aug 2015 09:02:56 -1000 (HST) 5, 22 -- Received: from localhost (tina-at-localhost) 5, 22 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id t7FJ2tEx007287; 5, 22 -- Sat, 15 Aug 2015 09:02:55 -1000 (HST) 5, 22 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 5, 22 -- Date: Sat, 15 Aug 2015 09:02:55 -1000 (HST) 5, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 22 -- X-X-Sender: tina-at-sf-v240 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , tomw-at-uidaho.edu 5, 22 -- Subject: Re: [Microscopy] SEM imaging of starch grains 5, 22 -- In-Reply-To: {201508151335.t7FDZiJO015922-at-ns.microscopy.com} 5, 22 -- Message-ID: {Pine.GSO.4.64.1508150856110.6679-at-sf-v240} 5, 22 -- References: {201508151335.t7FDZiJO015922-at-ns.microscopy.com} 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Starch grains are fun. They can be tough little buggers. I did some from barley in the past - the USDA people in the barley lab (because of the brewing industry in Wisconsin). What are you trying to image? Specimen prep: If dry, like corn kernels, just break open the kernel. Cryofracture is fun, but not needed. Poke out the starchy endosperm and spread it on the stub. Sputter coat with Au/Pd as per usual.
If wet - dissected from fresh, moist grains, then: Either dissect and allow to air dry - you won't affect the structure of the starch grains themselves and treat as above or fix with a normal formaldedhyde/glut fix, use an extended - like an hour or more - dehydration series and critical point dry. Dissect some more and spread on a stub.
If they're looking at how the grain is digested once the seed germinates - as the barley people were, then you must fix and dehydrate. But! It's also worth going the simple "do as little as possible" route. Starch grains are very tough and are very hard to break open - hitting with a hammer just produces individual grains. I've even tried cryofracture and not broken open a grain. But, the seeds do use enzymes and "open" the starch grains, producing pits. The walls of the pits have really neat light-dark layering.
Starch grains left behind by baking, I don't know. I've looked at bread dough and not seen starch grains, nor did I see any in the (fully baked) pretzels I did. Donuts, though ... there's a hole in my studies, there.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
________________________________________ X-from: zaluzec.microscopy.com-at-gmail.com [zaluzec.microscopy.com-at-gmail.com] Sent: Saturday, August 15, 2015 10:13 AM To: Oshel, Philip Eugene
X-from: tomw-at-uidaho.edu
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] SEM imaging of starch grains
Message: Greetings,
I have a user from our Food Sciences Dept with various starch specimens in need of SEM imaging. Initial attempts were made with no specific prep other than carbon coating [using an evaporative rod coater]. Results were okay but not great. Grains were often fractured and would rupture under the beam after approx 30 seconds of exposure [used a 5 kV AV under normal High Vac conditions].
This geologist humbly requests input or suggested sample prep procedures for starch [such as metal sputter coating vs carbon coating, or drying/dessication??].
My experience with starch is eating a donut.....
Any assistance would be greatly appreciated!
Cheers, Tom Williams
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==============================Original Headers============================== 28, 38 -- From oshel1pe-at-cmich.edu Sat Aug 15 19:16:56 2015 28, 38 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 28, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7G0Gu7n030886 28, 38 -- for {microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 19:16:56 -0500 28, 38 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 28, 38 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t7G0Gtlm022443; 28, 38 -- Sat, 15 Aug 2015 20:16:55 -0400 28, 38 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 28, 38 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 28, 38 -- (TLS) id 14.3.248.2; Sat, 15 Aug 2015 20:16:54 -0400 28, 38 -- Received: from cmail106.central.cmich.local ([fe80::a8:3e31:c722:be0a]) by 28, 38 -- CAS4.central.cmich.local ([fe80::cdc8:8010:7a7a:aa21%11]) with mapi id 28, 38 -- 14.03.0248.002; Sat, 15 Aug 2015 20:16:54 -0400 28, 38 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 28, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 28, 38 -- CC: "tomw-at-uidaho.edu" {tomw-at-uidaho.edu} 28, 38 -- Subject: RE: [Microscopy] viaWWW:SEM imaging of starch grains 28, 38 -- Thread-Topic: [Microscopy] viaWWW:SEM imaging of starch grains 28, 38 -- Thread-Index: AQHQ12+srl/YWd1z0UWRXtb6eyUetZ4NvD0J 28, 38 -- Date: Sun, 16 Aug 2015 00:16:53 +0000 28, 38 -- Message-ID: {FB5ED57CF3415D4F8C5F93F1DE5468732F79F2DA-at-cmail106.central.cmich.local} 28, 38 -- References: {201508151413.t7FEDA6d022113-at-ns.microscopy.com} 28, 38 -- In-Reply-To: {201508151413.t7FEDA6d022113-at-ns.microscopy.com} 28, 38 -- Accept-Language: en-US 28, 38 -- Content-Language: en-US 28, 38 -- X-MS-Has-Attach: 28, 38 -- X-MS-TNEF-Correlator: 28, 38 -- x-originating-ip: [141.209.2.100] 28, 38 -- Content-Type: text/plain; charset="us-ascii" 28, 38 -- MIME-Version: 1.0 28, 38 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 28, 38 -- X-Spam-Score: -4.60 () [Hold at 6.00] L_CTCMUM,L_EXCH_MF,RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 28, 38 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 28, 38 -- X-CanItPRO-Stream: default 28, 38 -- X-Canit-Stats-ID: 05P5cgTkO - cfe81a6a3cd6 - 20150815 28, 38 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 28, 38 -- Content-Transfer-Encoding: 8bit 28, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7G0Gu7n030886 ==============================End of - Headers==============================
Is there a reason they haven't tried the old fashioned way and looked at these samples with crossed polars in a light microscope? Starch grains give a distinctive maltese cross.
Good hunting, Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education ... "Education, not Training" 7101 Royal Glen Trail, Suite A - McKinney, TX 75070 - P: 972-924-5310 www.MicroscopyEducation.com
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At 02:26 PM 8/15/2015, you wrote:
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==============================Original Headers============================== 12, 32 -- From bfoster-at-the-mip.com Sat Aug 15 20:08:48 2015 12, 32 -- Received: from nm8-vm5.access.bullet.mail.gq1.yahoo.com (nm8-vm5.access.bullet.mail.gq1.yahoo.com [216.39.63.126]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7G18mjS020370 12, 32 -- for {Microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 20:08:48 -0500 12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1439687328; bh=hcUHo8R90PaaMhOihh5X8G995PgcPJXfMa/4QXlQ5UQ=; h=Date:To:From:Subject:In-Reply-To:References:From:Subject; b=EgYORuCFIkQ0L9xZMxrgtufD/fAA9qxZ8BGppuzJ/GrO6YxEs2BbTBjRUMMfz6KFFBI+DmJW9GvTbeJbm0ugrXSXQe7cJg6W8NdC/mlV3uI6ff/xiMP/26HcLpxdIbZs3akJxfzjWJIECRM4dV4/0WrWpZubzKRu/XArwFs7DqbtT404CggnXh9+n2EZEWj5/QIVR9JEBR4XTtfmKpi+QZpmIYDbqlyF0TLI5DYcNBSDar0wENWBwX+8mcyGIEOgHGucJjTZgtN9VnjqyZYFpzRuQe1ODKMlOFXaRYaxttjIzgfw6ZNFuOvc5p74xIKQIRIxsiaXi29Z5aLx3Ck2Gw== 12, 32 -- Received: from [216.39.60.168] by nm8.access.bullet.mail.gq1.yahoo.com with NNFMP; 16 Aug 2015 01:08:48 -0000 12, 32 -- Received: from [67.195.22.119] by tm4.access.bullet.mail.gq1.yahoo.com with NNFMP; 16 Aug 2015 01:08:48 -0000 12, 32 -- Received: from [127.0.0.1] by smtp114.sbc.mail.gq1.yahoo.com with NNFMP; 16 Aug 2015 01:08:48 -0000 12, 32 -- X-Yahoo-Newman-Id: 317686.4165.bm-at-smtp114.sbc.mail.gq1.yahoo.com 12, 32 -- Message-ID: {317686.4165.bm-at-smtp114.sbc.mail.gq1.yahoo.com} 12, 32 -- X-Yahoo-Newman-Property: ymail-3 12, 32 -- X-YMail-OSG: 1DAC5cAVM1nbtvxiKLBMNMoRtl1sB4GgVCxAKJm74CPFOMN 12, 32 -- baZpf7v2IFBlQlqoblknXjuplz0p7vxV968DOMtWt9tnQ9F9lF2hRWjeNinV 12, 32 -- hXyMv0bRkJDMW98p2SkKiDeOdtUlrbBlRQ8iZurAsJemrK3qz.jwlVxwYeic 12, 32 -- jW8lNzMHw.Y.d0cEnRaadQMEPnNLgIVQpc8z3NuQqMaQwyngHPafMnZDjWqg 12, 32 -- KyDwyrFQPvvFDe.JZXbe2SFJMLjS2tmZRnznM0qdjUIlay92pW0EuCil1hX9 12, 32 -- StLUBPubGlE5YY51ratme1JUgjHL7Cy3nDOuih3EtfqvWTe7q0vS70GTH2.H 12, 32 -- uKVYRQJFczsqtSUE0fr2ApJ5FYdU4xEHA7mtAFby6fWd8bINgyBGmMGkx.JI 12, 32 -- t16ARxVC4dB9XzkbGxqW_uLKMSqPuocYHPrYHAOTN024K8HonucnXGyziHKY 12, 32 -- lg64E2vl.vB0hxPWMC6ur43MUPbP4Lu0ih4kHpwIAJ5MUgcM2o6IBuW6mbTG 12, 32 -- FdbLqL6urEKJG3xpT4BJdc9pWce414bCV.BtIj3nbfIOyyf640AA4Lw-- 12, 32 -- X-Yahoo-SMTP: KOmzyESswBATZkB2214rZDIfSSNffJ2urJX_LO8NW63iukNVtw-- 12, 32 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 32 -- Date: Sat, 15 Aug 2015 20:01:32 -0500 12, 32 -- To: tina-at-pbrc.hawaii.edu, 12, 32 -- "MIcroscopy-Microscopy.com" {Microscopy-at-microscopy.com} 12, 32 -- From: Barbara Foster {bfoster-at-the-mip.com} 12, 32 -- Subject: Re: [Microscopy] Re: SEM imaging of starch grains 12, 32 -- In-Reply-To: {201508151913.t7FJDl1W008796-at-ns.microscopy.com} 12, 32 -- References: {201508151913.t7FJDl1W008796-at-ns.microscopy.com} 12, 32 -- Mime-Version: 1.0 12, 32 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
But for imaging starch breakdown - seeing the holes develop, and also for getting a quicker idea of the relative size, shape and abundance of small and large granules, SEM is quick, and you can keep the samples and look at them again if necessary.
Here, people from the starch lab extract the starch, wash to remove protein and other contaminants, dry it, spread on a sticky carbon tab on a stub, image with BSE at 10 Pa (no coating necessary). For higher magnification or resolution, gold-coat then image at 20 kV under HV.
Generally no fixation or other processing because in the various rinse steps you tend to lose the smaller B-granules which people here are very interested in. It's also easy to do and this way once trained, the starch folk can do this without needing my input.
Just have to make sure not to dwell on the grains too much when focussing or they tend to crack, especially if uncoated.
cheers, Rosemary
On 16/08/15 11:15 AM, "bfoster-at-the-mip.com" {bfoster-at-the-mip.com} wrote:
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==============================Original Headers============================== 10, 44 -- From prvs=663ea08eb=Rosemary.White-at-csiro.au Sat Aug 15 20:29:22 2015 10, 44 -- Received: from act-MTAout5.csiro.au (act-MTAout5.csiro.au [150.229.7.42]) 10, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7G1TLof008812 10, 44 -- for {Microscopy-at-microscopy.com} ; Sat, 15 Aug 2015 20:29:21 -0500 10, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 10, 44 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; 10, 44 -- t=1439688561; x=1471224561; 10, 44 -- h=from:to:cc:subject:date:message-id:in-reply-to: 10, 44 -- content-id:content-transfer-encoding:mime-version; 10, 44 -- bh=a64hNZ3CLcltU+I+sBw4dWb7sHitEC83bxMCovdsMbY=; 10, 44 -- b=BOeFLZKsRZi7uEEIRwg1TRuJ5S4w7hXuv1ipoCvh2rJF2volKS8IhuMQ 10, 44 -- M2Lja43G0c48uvaXWNOjftuoO+tx+axe9sk6MBaH8YZ+cFZfeITC1AD3h 10, 44 -- 9hrRnI8fhLqTO6j; 10, 44 -- X-SBRS: 4.5 10, 44 -- X-IronPort-Anti-Spam-Filtered: true 10, 44 -- X-IronPort-Anti-Spam-Result: A2FyDADj489VkRj3U5haA4JvgQBpBq1ehzGKJV2BcYJGgUICgS48EAEBAQEBAQEDDgEBAQEUEiEugiYgDAYEAi4BCAQJAQEBEQITGAQPBgwCGQEBAQQ6FCsSAQgRAwECAQwKAQcxEAETAQMBBQgCBAoEBRYGAQSHeAMSAQzIZgMKhVcBAQEBAQEBAwEBAQEBAQEBAQEBF4pPgQOCTw0aAYEvEAIBHQgbEAcGDAEBhBgFhWwMgSmKcIMMhQSFKVKBbYIQkGEKhyyEI3EBAQEBgQMHFyOBBAEBAQ 10, 44 -- X-IPAS-Result: A2FyDADj489VkRj3U5haA4JvgQBpBq1ehzGKJV2BcYJGgUICgS48EAEBAQEBAQEDDgEBAQEUEiEugiYgDAYEAi4BCAQJAQEBEQITGAQPBgwCGQEBAQQ6FCsSAQgRAwECAQwKAQcxEAETAQMBBQgCBAoEBRYGAQSHeAMSAQzIZgMKhVcBAQEBAQEBAwEBAQEBAQEBAQEBF4pPgQOCTw0aAYEvEAIBHQgbEAcGDAEBhBgFhWwMgSmKcIMMhQSFKVKBbYIQkGEKhyyEI3EBAQEBgQMHFyOBBAEBAQ 10, 44 -- X-IronPort-AV: E=Sophos;i="5.15,687,1432562400"; 10, 44 -- d="scan'208";a="70620044" 10, 44 -- Received: from exhtca04-cdc.nexus.csiro.au ([152.83.247.24]) 10, 44 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES256-SHA; 16 Aug 2015 11:29:19 +1000 10, 44 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80::9de3:d7af:4ed1:b7b6]) by 10, 44 -- exhtca04-cdc.nexus.csiro.au ([fe80::7414:eafc:a0d3:3063%13]) with mapi id 10, 44 -- 14.03.0224.002; Sun, 16 Aug 2015 11:29:19 +1000 10, 44 -- From: {Rosemary.White-at-csiro.au} 10, 44 -- To: {Microscopy-at-microscopy.com} 10, 44 -- CC: {tomw-at-uidaho.edu} 10, 44 -- Subject: Re: [Microscopy] SEM imaging of starch grains 10, 44 -- Thread-Topic: [Microscopy] SEM imaging of starch grains 10, 44 -- Thread-Index: AQHQ18EPVvXTi0RILUS5ThWebYGRfp4N1myA 10, 44 -- Date: Sun, 16 Aug 2015 01:29:19 +0000 10, 44 -- Message-ID: {D1F6221F.1A5474%rosemary.white-at-csiro.au} 10, 44 -- In-Reply-To: {201508160115.t7G1FNJ5027405-at-ns.microscopy.com} 10, 44 -- Accept-Language: en-NZ, en-US, en-AU 10, 44 -- Content-Language: en-US 10, 44 -- X-MS-Has-Attach: 10, 44 -- X-MS-TNEF-Correlator: 10, 44 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 10, 44 -- x-originating-ip: [152.83.195.117] 10, 44 -- Content-Type: text/plain; charset="us-ascii" 10, 44 -- Content-ID: {98D7D18854B0584F86B5820835373F89-at-csiro.au} 10, 44 -- MIME-Version: 1.0 10, 44 -- Content-Transfer-Encoding: 8bit 10, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7G1TLof008812 ==============================End of - Headers==============================
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Now that all are perhaps back from M&M, I'm posting this message again in hopes of some responses.
I'd really like to find some LEO/Zeiss users who are way more versed in SmartSEM macros than myself. I'm trying to edit a macro to take scans with scan speed and line integration values according to specific store resolutions. This seemed pretty straightforward but it got really problematic very quickly.
The image storing I'm using is line integration for noise reduction, and depending on the store resolution, the scan speed and line integration N are changed to result in about a one minute total scan time for each store resolution. The macro I have thus far is:
Macro Name : photo2 Version 1.0 /* GDG July 2015 */ Unfreeze All Freeze on = End Frame If : Store resolution = 3072 * 2304 Is True Line Int. N= 8 Scan Speed 5 EndIf Statement If : Store resolution = 2048 * 1536 Is True Line Int. N= 6 Scan Speed 6 EndIf Statement If : Store resolution = 1024 * 768 Is True Line Int. N= 7 Scan Speed 8 EndIf Statement If : Store resolution = 512 * 384 Is True Line Int. N= 12 Scan Speed 9 EndIf Statement End Macro PHOTO2
I have a drop down Menu for Store Resolution but I cannot figure out how to feed this into the macro before the first If statement. I tried Else and other decision statements but this macro is close to working but not working. If I set the Store Resolution in the Scanning tab and then just run the macro, it works fine. But I'm looking for a way to specify the resolution at the beginning and have that fed into the Photo2 macro.
Does anyone have any suggestions about how to accomplish this task?
All inputs are appreciated. TIA.
gary g.
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We invite you to submit Focus Topic Invited Speaker Nominations for the session on âC/omplex Oxide Interfaces and Heterostructures/â (DMP, 13.1.4) at the 2016 American Physical Society March Meeting (Baltimore, Maryland, March 14 - March 18, 2016, USA).
*Deadline for Nominations:*August 17, 2015
*Focus Topic Description:*Complex oxide heterostructures display a range of impressive multi-functionality, encompassing superconductivity, colossal magnetoresistance, magnetism, multiferroicity, and strongly correlated Mott-Hubbard insulator-type behavior in addition to novel interface-stabilized ground states such as two-dimensional electron gases (2DEGs), 2D superconductivity, novel magnetism, and topological phases. The extreme sensitivity of these phenomena to composition and interface structure offers endless possibilities for fundamental studies of the interactions between the structural and electronic degrees of freedom that give rise to these fascinating phenomena, and thus providing many insights into materials physics in addition to the capability to design completely new devices. Local symmetry breaking, charge transfer, magnetic and electrostatic interactions, and coupling between structural modes are just some of the many mechanisms that can lead to the appearance of novel interfacial functionalities and can be employed for rational design of artificial materials with desirable structural, electronic and magnetic properties.
The aim of this focus session is to provide a forum for the discussion of recent experimental and theoretical results on complex-oxide heterostructures and their interfaces. The topics covered in this session will include advances in the growth and characterization of complex-oxide heterostructures, development of interface-related measurement techniques, theory and modeling of oxide heterostructures and interfaces, experimental investigation and tuning of interface-related properties in conducting, insulating and magnetic oxides, chemical and electrochemical effects in manifestation of physical functionalities, applications based on interface-related phenomena in complex-oxide heterostructures, and new phenomena appearing in complex oxides owing to heterostructuring.
*Instructions:*Suggestions for invited speakers should be submitted at the APS nomination website, {http://www.aps.org/units/dmp/meetings/invited.cfm} http://www.aps.org/units/dmp/meetings/invited.cfm. Please select category,/13.1.4 Complex Oxide Interfaces and Heterostructures (DMP),/and complete the nomination form in full.
Your nomination will go to the organizers of the Focus Topic for which you have suggested a candidate and will aid the organizers in their selection of invited speakers. In suggesting speakers, please keep in mind that speakers who gave an invited talk at the previous March Meeting are ineligible (2015 March Meeting invited speakers: {http://meetings.aps.org/Meeting/MAR15/APS_Invited} http://meetings.aps.org/Meeting/MAR15/APS_Invited).
On behalf of the symposium organizers,
James Rondinelli, Northwestern University Sergei V. Kalinin, Oak Ridge National Laboratory
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Theme Leader, Center for Nanophase Materials Science
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 14, 25 -- From sergei2-at-ornl.gov Mon Aug 17 12:24:02 2015 14, 25 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7HHO2s2007125 14, 25 -- for {microscopy-at-microscopy.com} ; Mon, 17 Aug 2015 12:24:02 -0500 14, 25 -- X-SG: RELAYLIST 14, 25 -- X-IronPort-AV: E=Sophos;i="5.15,696,1432612800"; 14, 25 -- d="scan'208";a="86821586" 14, 25 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 14, 25 -- by iron2.ornl.gov with ESMTP/TLS/ADH-AES256-GCM-SHA384; 17 Aug 2015 13:24:01 -0400 14, 25 -- Received: from [128.219.192.116] (pc95228.ornl.gov [128.219.192.116]) 14, 25 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 14, 25 -- (No client certificate requested) 14, 25 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 3mw2KY3BLGz6wHZ 14, 25 -- for {microscopy-at-microscopy.com} ; Mon, 17 Aug 2015 13:24:01 -0400 (EDT) 14, 25 -- To: microscopy-at-microscopy.com 14, 25 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 14, 25 -- Subject: Call for Invited Speaker Nominations: APS 2016 March Meeting Focus 14, 25 -- Topic on Complex Oxide Interfaces and Heterostructures 14, 25 -- Message-ID: {55D218B1.7030802-at-ornl.gov} 14, 25 -- Date: Mon, 17 Aug 2015 13:24:01 -0400 14, 25 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 14, 25 -- Thunderbird/38.0.1 14, 25 -- MIME-Version: 1.0 14, 25 -- Content-Type: text/plain; charset=utf-8; format=flowed 14, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From ohn.bullocknewsbbug-at-gmail.com Mon Aug 17 13:47:11 2015 Return-Path: {ohn.bullocknewsbbug-at-gmail.com} Received: from gmail.com ([121.141.172.242]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t7HIl81c029148 for {microscopylistserverarchive5-at-microscopy.com} ; Mon, 17 Aug 2015 13:47:10 -0500 Message-ID: {BD4338C2.65F6FBFE-at-gmail.com}
Greetings colleagues,
I am in a slightly frustrating situation. I have a FEI Morgagni 268D TEM 100kV. We are trying to get both Megaview III and Tengra cameras (both made by Emsis, formerly OIS, formerly SIS) to run with OIS iTEM, but it is proving very difficult.
Have you ever seen a Morgagni control PC running XP or Win7? Anyone ever had experience getting these cameras to talk together on the same system? I'd be delighted to hear from you either on forum or privately.
It is definitely a software issue as we can get both cameras working on a Win7 PC separately and even together they are both briefly recognised, but iTEM software becomes unusable as soon as one or the other is selected.
Current likely solution is to have 2 pcs running, one controlling TEM and Megaview III and the other just with the Tengra (we will have to manually input mag etc. when we shoot and do manual montaging).
Other details: The control PC communicates via serial links to the microscope (via a DOS-like eprom pc).
The control PC runs Windows 2000 which, I am told by FEI, is the latest operating system that the control software should officially be run on.
Our MegaViewIII has had a firmware upgrade that will allow compatibility with later OSes, but not the hardware upgrade to G2 camera.
Hoping someone out there has been crazy enough to try this before? Duane
AgResearch Limited | Lincoln Research Centre | New Zealand Dr Duane P Harland, Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz ======================================================================= Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately. =======================================================================
==============================Original Headers============================== 11, 35 -- From Duane.Harland-at-agresearch.co.nz Mon Aug 17 15:55:55 2015 11, 35 -- Received: from mx1.securemx.biz (mx1.securemx.biz [203.84.134.2]) 11, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7HKtssj032389 11, 35 -- for {microscopy-at-microscopy.com} ; Mon, 17 Aug 2015 15:55:54 -0500 11, 35 -- DKIM-Signature: v=1; a=rsa-sha256; d=nz.smxemail.com; s=alpha; c=relaxed/relaxed; 11, 35 -- q=dns/txt; i=-at-nz.smxemail.com; t=1439844953; 11, 35 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc; 11, 35 -- bh=HwdjzeKogOCvn7JgvlkMCzjwdWdEz0ySRilenruURe8=; 11, 35 -- b=LuFhlBJrxjWKvyQyvDrtf5ptY2bkuMhSZijgMEhW5T6TVKIm+AJbfPge3fGd6W/i 11, 35 -- 8jJijUM3BzNoZ1ZyoDFMqGRVNsT8lOV0dwGiwqYj4u3rRAoOoRkPcumWipCEuQhh 11, 35 -- JhgW9lhlOuoIL3JUbxHQUER7oOIJ5JBbgRLucozPip8=; 11, 35 -- Received: from invexcpv02.agresearch.co.nz ([202.20.102.50]) 11, 35 -- by omr.nz.smxemail.com with ESMTP (using TLSv1 11, 35 -- with cipher AES128-SHA (128/128 bits)) 11, 35 -- id 55D24A59-6CB30250-at-mta1103.omr; 11, 35 -- Mon, 17 Aug 2015 20:55:53 +0000 11, 35 -- Received: from INVEXCPV01.agresearch.co.nz ([169.254.1.202]) by 11, 35 -- invexcpv03.agresearch.co.nz ([147.158.130.6]) with mapi id 14.03.0210.002; 11, 35 -- Tue, 18 Aug 2015 08:55:50 +1200 11, 35 -- From: "Harland, Duane" {Duane.Harland-at-agresearch.co.nz} 11, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 35 -- Subject: Morgagni + MegaVIII + Tengra, possible? 11, 35 -- Thread-Topic: Morgagni + MegaVIII + Tengra, possible? 11, 35 -- Thread-Index: AdDZLxh8Ig/IZw+YTnW9jCWh55wg+Q== 11, 35 -- Date: Mon, 17 Aug 2015 20:55:49 +0000 11, 35 -- Message-ID: {C1CB2BCA7689064DA55A366147C8BEF18D2779FB-at-invexcpv01.agresearch.co.nz} 11, 35 -- Accept-Language: en-GB, en-NZ, en-US 11, 35 -- Content-Language: en-US 11, 35 -- X-MS-Has-Attach: 11, 35 -- X-MS-TNEF-Correlator: 11, 35 -- x-originating-ip: [147.158.192.206] 11, 35 -- Content-Type: text/plain; charset="us-ascii" 11, 35 -- MIME-Version: 1.0 11, 35 -- Content-Transfer-Encoding: 8bit 11, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7HKtssj032389 ==============================End of - Headers==============================
Hi all, Does anyone in the Memphis, TN area do plunge freezing and freeze substitution? or high pressure freezing? Any cryo at Graceland? ;-) thank you, thank you very much, Beth
==============================Original Headers============================== 1, 46 -- From bethrichardson-at-uga.edu Tue Aug 18 10:23:06 2015 1, 46 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1on0144.outbound.protection.outlook.com [157.56.110.144]) 1, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7IFN6sL011759 1, 46 -- for {microscopy-at-microscopy.com} ; Tue, 18 Aug 2015 10:23:06 -0500 1, 46 -- Received: from BLUPR02MB212.namprd02.prod.outlook.com (10.242.189.151) by 1, 46 -- BLUPR02MB210.namprd02.prod.outlook.com (10.242.189.145) with Microsoft SMTP 1, 46 -- Server (TLS) id 15.1.231.21; Tue, 18 Aug 2015 15:23:03 +0000 1, 46 -- Received: from BLUPR02MB212.namprd02.prod.outlook.com ([169.254.1.247]) by 1, 46 -- BLUPR02MB212.namprd02.prod.outlook.com ([169.254.1.247]) with mapi id 1, 46 -- 15.01.0231.024; Tue, 18 Aug 2015 15:23:03 +0000 1, 46 -- From: Beth Richardson {bethrichardson-at-uga.edu} 1, 46 -- To: microscopy microscopy {microscopy-at-microscopy.com} 1, 46 -- Subject: plunge freezing in Memphis? 1, 46 -- Thread-Topic: plunge freezing in Memphis? 1, 46 -- Thread-Index: AQHQ2cnGxuToNRh2wkaweGVTyXAQqg== 1, 46 -- Date: Tue, 18 Aug 2015 15:23:03 +0000 1, 46 -- Message-ID: {F7700440-F15D-43C2-957C-B46AABA276DD-at-uga.edu} 1, 46 -- Accept-Language: en-US 1, 46 -- Content-Language: en-US 1, 46 -- X-MS-Has-Attach: 1, 46 -- X-MS-TNEF-Correlator: 1, 46 -- authentication-results: spf=none (sender IP is ) 1, 46 -- smtp.mailfrom=bethrichardson-at-uga.edu; 1, 46 -- x-originating-ip: [198.137.20.110] 1, 46 -- x-microsoft-exchange-diagnostics: 1;BLUPR02MB210;5:w+OOv6G8s3y1Cw3JgUICeAEm2yTG/n502Cz9a+N0JMiY9JTd1fTwhwxNCK5WOH3hJ/coy+YPXzBVr/GisTXo2hkuhKfA/NchHnbh75r6sBTwCTsq46XuMM3vgF/lGSwyHXzamxdBOL3bzF4wrdl9gQ==;24:0Bou56bsGRPdMPt7r3hoxodDiVexDWIqilIbjz465SAJsrNEPYNMsUotbCHJImcN33bEP5KZQX1VL/eZij1DBADr8tis7F75XPqQKvsQzgI=;20:0bKD30c4eYDqPvh7+QWQGiP6siGvj8G9ttQEHEkGh8hBzzhG1jL69uVECoZlM5DZl4UlT2JJ48ZA+gYAJWdymg== 1, 46 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BLUPR02MB210; 1, 46 -- x-microsoft-antispam-prvs: {BLUPR02MB21054F24FB4DC8BCC60BAF4DD780-at-BLUPR02MB210.namprd02.prod.outlook.com} 1, 46 -- x-exchange-antispam-report-test: UriScan:; 1, 46 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(8121501046)(5005006)(3002001);SRVR:BLUPR02MB210;BCL:0;PCL:0;RULEID:;SRVR:BLUPR02MB210; 1, 46 -- x-forefront-prvs: 067270ECAF 1, 46 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(53754006)(189002)(45984002)(199003)(40100003)(88552001)(10400500002)(86362001)(558084003)(50986999)(450100001)(68736005)(122556002)(229853001)(75432002)(2656002)(89122001)(33656002)(82746002)(54356999)(101416001)(83716003)(46102003)(2900100001)(85806002)(77156002)(106356001)(66066001)(90282001)(106116001)(62966003)(99286002)(87936001)(105586002)(4000370100001)(189998001)(102836002)(5001860100001)(5001960100002)(107886002)(36756003)(5002640100001)(81156007)(97736004)(64706001)(110136002)(4001540100001)(5001830100001)(92566002)(104396002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR02MB210;H:BLUPR02MB212.namprd02.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:3;A:1;LANG:en; 1, 46 -- received-spf: None (protection.outlook.com: uga.edu does not designate 1, 46 -- permitted sender hosts) 1, 46 -- spamdiagnosticoutput: 1:23 1, 46 -- spamdiagnosticmetadata: NSPM 1, 46 -- Content-Type: text/plain; charset="us-ascii" 1, 46 -- Content-ID: {F705109FFF9E074F9586414D9F341B07-at-namprd02.prod.outlook.com} 1, 46 -- MIME-Version: 1.0 1, 46 -- X-OriginatorOrg: uga.edu 1, 46 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 18 Aug 2015 15:23:03.5468 1, 46 -- (UTC) 1, 46 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 1, 46 -- X-MS-Exchange-CrossTenant-id: a8216c1e-4d63-4352-8c3b-50fa1f1475b1 1, 46 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BLUPR02MB210 1, 46 -- Content-Transfer-Encoding: 8bit 1, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7IFN6sL011759 ==============================End of - Headers==============================
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Email: ngr-at-ufl.edu Name: Nicholas Rudawski
Organization: University of Florida
Title-Subject: [Filtered] used digital camera for JEOL 200CX
Message: Hello everyone:
Does anyone happen to have a working TEM digital camera and is looking to sell it? We have a 16 year old Gatan MSC 794 mounted on a JEOL 200CX; the camera is starting to malfunction and Gatan cannot guarantee it can be repaired since it is discontinued, so we are looking for a replacement.
Thanks,
Nick
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Wed Aug 19 10:19:58 2015 14, 40 -- Received: from mail-io0-f181.google.com (mail-io0-f181.google.com [209.85.223.181]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7JFJw1w000523 14, 40 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 10:19:58 -0500 14, 40 -- Received: by iodt126 with SMTP id t126so12730210iod.2 14, 40 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 08:19:58 -0700 (PDT) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=p+BX3LyvUTprzTy9fud40nT4zvChiDEDnghLXWJgxmc=; 14, 40 -- b=yTmp+xyiS0FMSfE6WeqRJ53q+gIdEsCIKP7gBMdPvmvv5xsYwVReVggRAsEQIkiSZJ 14, 40 -- MEdl6U4SFsbnt1g7djGOFL6OHFriTAiQnxkPK8RejgATzi7Yzi7VuuyYN1f9g49jOr8I 14, 40 -- vt0MjtMx+ikcewngknl/riLuzu8rCjrQORZJC9hNqfZntl2sKkKF9xafe5EigDZC0+dS 14, 40 -- 4AKGN3/Y+iKrEZWAQfUptPQ0ITk/b7qDLRzfMZC1kXN1OXbT7JY8RAjppoAKyO2yugu8 14, 40 -- d26n67yV/CWCmdoUWGBVYfAOHuVQnZCP3t1TifACoymCmKcoPwpRiaPRKmYTsk9i2VDD 14, 40 -- QDpw== 14, 40 -- X-Received: by 10.107.8.11 with SMTP id 11mr11980828ioi.125.1439997598142; 14, 40 -- Wed, 19 Aug 2015 08:19:58 -0700 (PDT) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id vk8sm2353099igb.4.2015.08.19.08.19.57 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 40 -- Wed, 19 Aug 2015 08:19:57 -0700 (PDT) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:Looking for used digital camera for JEOL 200CX 14, 40 -- References: {201508171433.t7HEXClh001699-at-ns.microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- X-Forwarded-Message-Id: {201508171433.t7HEXClh001699-at-ns.microscopy.com} 14, 40 -- Message-ID: {55D49E9C.2080809-at-microscopy.com} 14, 40 -- Date: Wed, 19 Aug 2015 10:19:56 -0500 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.1.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201508171433.t7HEXClh001699-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: egelman-at-virginia.edu Name: Edward Egelman
Organization: University of Virginia
Title-Subject: [Filtered] Biophysical Society Cryo-EM Subgroup
Message: Dear Colleagues, Now that the Cryo-EM Subgroup of the Biophysical Society is official, it is time to start planning the first Symposium which we will hold in conjunction with the Annual Meeting in Los Angeles (27 February to 2 March, 2016). Like all subgroups, the Cryo-EM Subgroup Symposium will be held the day before the Annual Meeting: from 7:00 to 9:00 pm on Saturday, 27 February. We would like to have six talks, each 20 minutes including 5 minutes for questions. Following the talks, we will have a Business Meeting from 9:00 to 9:30 pm during which we will elect officers of the subgroup and decide on the format for future meetings. We want to guarantee a diverse group of speakers that includes students, postdocs and faculty. Due to the rapid pace of progress in cryo-EM these days, we thought that rather than inviting specific individuals, we would solicit abstracts with a deadline of 10 September. The site for abstract submission is:
https://www.surveymonkey.com/r/G5LLS3N
We will notify selected speakers by 16 September and they will be offered complimentary registration for the full Annual Meeting. Those who were not selected will be able to submit the same (or different) abstracts through the official Biophysical Society website, which has a 1 October deadline. We plan to also organize one or more Cryo-EM platform sessions during the Annual Meeting, the number of which will simply depend upon the number of abstracts submitted for the 1 October deadline.
Regards, The ad hoc committee for the Cryo-EM Subgroup Bridget Carragher Yifan Cheng Edward Egelman Irina Serysheva David Stokes Da-Neng Wang
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Message: We are decommissioning two research/demo tools; Hitachi3000H and FEI/Philips XL30. Willing to provide with or without new SDD EDS.
Please contact travisw-at-ixrfsystems.com if you are interested.
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==============================Original Headers============================== 16, 40 -- From microscopy.listserver-at-gmail.com Wed Aug 19 10:21:57 2015 16, 40 -- Received: from mail-io0-f174.google.com (mail-io0-f174.google.com [209.85.223.174]) 16, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7JFLqLP001521 16, 40 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 10:21:52 -0500 16, 40 -- Received: by iodt126 with SMTP id t126so12801646iod.2 16, 40 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 08:21:51 -0700 (PDT) 16, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 40 -- d=gmail.com; s=20120113; 16, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 16, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 16, 40 -- bh=8PEsDPg0CTiOkKrTXNxD3rR/555tUO/IHqBksm7Cb1k=; 16, 40 -- b=sfb4Z5t0JOwUpLhDmjWQdprFKs/dTckzAqp6UyyKsYUzzhrgsIVcsyvCzIxb/SLCXx 16, 40 -- HmRg5FYyD8O/MnC/KYLpDs9vr1p30Rzg4afTv6dBPqnRA4h99UNGhnb8yq5Xf2NFVty7 16, 40 -- auc9WaFkxgXT3QQmCBKLSTTpybEXPFywyV6gmrdt39MoYH/kFxm1jJklayLy7m8/mt/V 16, 40 -- kmEVfEHVp910z+UlVGfJ+G0D5Zxn1G3f7AjxYhjkEjUX9IBxbEMIYa/imHiqQQ4zoEL3 16, 40 -- xxmq+qg9fdRuR2MQhijl4fiwb2KpA+yFPrBvZufjt7/IPh9A8dKIAadabwyuoevx0OFo 16, 40 -- fDWg== 16, 40 -- X-Received: by 10.107.34.81 with SMTP id i78mr13102450ioi.40.1439997711587; 16, 40 -- Wed, 19 Aug 2015 08:21:51 -0700 (PDT) 16, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 16, 40 -- by smtp.googlemail.com with ESMTPSA id sa6sm2346681igb.13.2015.08.19.08.21.51 16, 40 -- for {microscopy-at-microscopy.com} 16, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 40 -- Wed, 19 Aug 2015 08:21:51 -0700 (PDT) 16, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 16, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 40 -- {microscopylistserver-noreply-at-microscopy.com} 16, 40 -- Subject: viaWWW:Decommissioning Scopes 16, 40 -- References: {201508181610.t7IGARkN001485-at-ns.microscopy.com} 16, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 40 -- X-Forwarded-Message-Id: {201508181610.t7IGARkN001485-at-ns.microscopy.com} 16, 40 -- Message-ID: {55D49F0E.3040200-at-microscopy.com} 16, 40 -- Date: Wed, 19 Aug 2015 10:21:50 -0500 16, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 16, 40 -- Gecko/20100101 Thunderbird/38.1.0 16, 40 -- MIME-Version: 1.0 16, 40 -- In-Reply-To: {201508181610.t7IGARkN001485-at-ns.microscopy.com} 16, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM Workshop
Message: The Georgia Electron Microscope center at the University of Georgia will be holding an intensive, three day workshop on Biological Transmission Electron Microscopy. October 12-14, 2015. Participation is limited to six people and the deadline is October 1.
Information on registration can be found at GEM.uga.edu and a flyer with more information, cost and other details can be obtained from John Shields at jpshield-at-uga.edu
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==============================Original Headers============================== 15, 40 -- From microscopy.listserver-at-gmail.com Wed Aug 19 10:22:44 2015 15, 40 -- Received: from mail-io0-f180.google.com (mail-io0-f180.google.com [209.85.223.180]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7JFMhBg002196 15, 40 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 10:22:43 -0500 15, 40 -- Received: by iodt126 with SMTP id t126so12833956iod.2 15, 40 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 08:22:43 -0700 (PDT) 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 40 -- d=gmail.com; s=20120113; 15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 40 -- bh=Hyi7bZlYwc8CsCEeB1ZXcTUFzFlib0s397wz6ZWFwiM=; 15, 40 -- b=DYaVAsh1gTRZujBkWCPQIsd0bB3jijEbpPkneglUFwV+uFEz2yeNxdCS21Kghc1VMS 15, 40 -- zxgxRnidTxYEUQo1l9493dcjwhRAdVKtG0XWQnDaAkgz3HV/enj9ZT5SSao7gn7JSBNI 15, 40 -- NPdk27pRLIW3I2vxMNjQw7of/+MldAJ7gralm6z32HszSDiJJEIT4uQM70kuEOQRX6g/ 15, 40 -- qtLAvAvw+eCxyhFKBg0S6VKMSjea7dTau7NLk+bR2r0P19HK/NRLCELBtRiVHxKI8IeL 15, 40 -- U6YwAo36ZGjx29wFekTWGujCXPgNt0alGPBsxeAtxHCnWCTgVtX69OeBdFQUVu29T+LE 15, 40 -- rxMg== 15, 40 -- X-Received: by 10.107.153.5 with SMTP id b5mr15350876ioe.143.1439997763481; 15, 40 -- Wed, 19 Aug 2015 08:22:43 -0700 (PDT) 15, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 40 -- by smtp.googlemail.com with ESMTPSA id q79sm848226ioi.0.2015.08.19.08.22.43 15, 40 -- for {microscopy-at-microscopy.com} 15, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 40 -- Wed, 19 Aug 2015 08:22:43 -0700 (PDT) 15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 40 -- {microscopylistserver-noreply-at-microscopy.com} 15, 40 -- Subject: viaWWW:Biological TEM Workshop -at-University of Georgia 15, 40 -- References: {201508181732.t7IHWP7l004052-at-ns.microscopy.com} 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 40 -- X-Forwarded-Message-Id: {201508181732.t7IHWP7l004052-at-ns.microscopy.com} 15, 40 -- Message-ID: {55D49F42.4090108-at-microscopy.com} 15, 40 -- Date: Wed, 19 Aug 2015 10:22:42 -0500 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 40 -- Gecko/20100101 Thunderbird/38.1.0 15, 40 -- MIME-Version: 1.0 15, 40 -- In-Reply-To: {201508181732.t7IHWP7l004052-at-ns.microscopy.com} 15, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] Thanks for all the Starch help!
Message: Greetings,
The help and suggestions for starch imaging where incredibly useful and generous. Many sent along detailed suggestions, steps, and helpful hints. After incorporating several of the tips I now have much better [and improving] images. I and my faculty member are much happier :)) .
Thanks again to all those who helped.
Cheers, Tom
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==============================Original Headers============================== 15, 40 -- From microscopy.listserver-at-gmail.com Wed Aug 19 10:23:22 2015 15, 40 -- Received: from mail-ig0-f182.google.com (mail-ig0-f182.google.com [209.85.213.182]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7JFNMpo002747 15, 40 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 10:23:22 -0500 15, 40 -- Received: by igui7 with SMTP id i7so110443443igu.1 15, 40 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 08:23:22 -0700 (PDT) 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 40 -- d=gmail.com; s=20120113; 15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 40 -- bh=kL0FXcO8UhIh2yneRgFrTDybMrb8Gf4txRJsxw6DnEU=; 15, 40 -- b=XT3RZqw5Cfe0lkCRfhjlSqcoXZh7X+GdXSJN5WiuB5sniRV1Ff4zvLqrB9Xld6bp9J 15, 40 -- /tW0Cy9Rabj7bXR6Gngkm1jcpOxej0NPAjf6Q0gAJC1tHWguWOysXQHAvvKA9VJJ3qWE 15, 40 -- ISFEgeldYcM4x+wyf2serfjKcLLVQ+Q5ZdxCRr12TEeD4l9dQJ1A9DoxLSyWDeFa51xB 15, 40 -- qJhY7aFXca2r59q+fm3PqjGvZ3QJfEtnwEYqHIygMPZrCiLjBg3wr39QZXbw5xiZfi6w 15, 40 -- MIsf+KMQYGPb4fWIslVhtZ3EUlUFpV0I/PAIjv0lvUAvpqVOXC0ti8g0waI27s4vqvfO 15, 40 -- hZFw== 15, 40 -- X-Received: by 10.50.82.10 with SMTP id e10mr9643701igy.73.1439997802410; 15, 40 -- Wed, 19 Aug 2015 08:23:22 -0700 (PDT) 15, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 40 -- by smtp.googlemail.com with ESMTPSA id q12sm15596336igr.2.2015.08.19.08.23.21 15, 40 -- for {microscopy-at-microscopy.com} 15, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 40 -- Wed, 19 Aug 2015 08:23:22 -0700 (PDT) 15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 40 -- {microscopylistserver-noreply-at-microscopy.com} 15, 40 -- Subject: viaWWW:Thanks for all the Starch help! 15, 40 -- References: {201508181837.t7IIbtvT005974-at-ns.microscopy.com} 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 40 -- X-Forwarded-Message-Id: {201508181837.t7IIbtvT005974-at-ns.microscopy.com} 15, 40 -- Message-ID: {55D49F69.9040603-at-microscopy.com} 15, 40 -- Date: Wed, 19 Aug 2015 10:23:21 -0500 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 40 -- Gecko/20100101 Thunderbird/38.1.0 15, 40 -- MIME-Version: 1.0 15, 40 -- In-Reply-To: {201508181837.t7IIbtvT005974-at-ns.microscopy.com} 15, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From advertise.bz222tnpv-at-gmail.com Wed Aug 19 12:48:32 2015 Return-Path: {advertise.bz222tnpv-at-gmail.com} Received: from gmail.com ([1.235.193.146]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t7JHmSST002844 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 19 Aug 2015 12:48:31 -0500 Message-ID: {99069289.DD43F3F3-at-gmail.com}
X-from: fengxia.liang-at-med.nyu.edu
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Email: fengxia.liang-at-med.nyu.edu Name: Alice Liang
Organization: NYULMC OCS Microscopy Core
Title-Subject: [Filtered] Biological sample preparation method for element analysis using EDX
Message: We have a project need to calculate calcium amount in the mitochondria of cultured cells. We tried once with SEM/EDX using thin section of routine TEM protocol of GA/OsO4/Epon with HEPES buffer, and result is not good. There are obvious some heavy mental contaminants, but we can't detect Calcium. Any suggestion?
Thanks, Alice NYULMC OCS Microscopy Core
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==============================Original Headers============================== 13, 41 -- From microscopy.listserver-at-gmail.com Wed Aug 19 18:30:23 2015 13, 41 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) 13, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7JNUNoV020587 13, 41 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 18:30:23 -0500 13, 41 -- Received: by iodt126 with SMTP id t126so27598362iod.2 13, 41 -- for {microscopy-at-microscopy.com} ; Wed, 19 Aug 2015 16:30:23 -0700 (PDT) 13, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 41 -- d=gmail.com; s=20120113; 13, 41 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 13, 41 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 13, 41 -- bh=8Xr2Vd1EpfnZxFVzD0UnDU+acfKwkkIqbNl+mXe95kk=; 13, 41 -- b=cgPAcYfydumzYxUrASpVk8dEKE1x9b8lmdBYDg/8MAyims7nof99GZBaZeJcsnc5n2 13, 41 -- hGFtDXu07TcKyvlwZIUJoZqlUwLwi+c4NS5T8OnU718eHZuVFhzVOTYS3z5bRK95j4FV 13, 41 -- my7XE/smXAc7ARwYyOMaQ//mAYX1hgRfbfTehZJCQmhwxIwsnn+wvkOjcI7ttJlCmvjY 13, 41 -- iAgL6gHWQZeQBLNM7prpZcaT4aufzPYQTDoeuDglcGrYcxIAZ+jS23yXMlj+vR36oVWj 13, 41 -- XGYQchqHzPJByKEQ5sAj6vyl/xvZTuC2hb4SLQRL8aiU0/qJ7SwqQ17BI8r+TZC70T5r 13, 41 -- +7EA== 13, 41 -- X-Received: by 10.107.135.152 with SMTP id r24mr76378ioi.142.1440027023428; 13, 41 -- Wed, 19 Aug 2015 16:30:23 -0700 (PDT) 13, 41 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 13, 41 -- by smtp.googlemail.com with ESMTPSA id 91sm1877684ioi.44.2015.08.19.16.30.22 13, 41 -- for {microscopy-at-microscopy.com} 13, 41 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 41 -- Wed, 19 Aug 2015 16:30:22 -0700 (PDT) 13, 41 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 13, 41 -- X-Google-Original-From: MicroscopyListserver-NoReply 13, 41 -- {microscopylistserver-noreply-at-microscopy.com} 13, 41 -- Subject: viaWWW:Biological sample preparation method for element analysis 13, 41 -- using EDX 13, 41 -- References: {201508191643.t7JGhPt2028164-at-ns.microscopy.com} 13, 41 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 41 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 41 -- X-Forwarded-Message-Id: {201508191643.t7JGhPt2028164-at-ns.microscopy.com} 13, 41 -- Message-ID: {55D5118D.7060201-at-microscopy.com} 13, 41 -- Date: Wed, 19 Aug 2015 18:30:21 -0500 13, 41 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 13, 41 -- Gecko/20100101 Thunderbird/38.1.0 13, 41 -- MIME-Version: 1.0 13, 41 -- In-Reply-To: {201508191643.t7JGhPt2028164-at-ns.microscopy.com} 13, 41 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: kpseverin-at-alaska.edu Name: Ken Severin
Organization: University of Alaska Fairbanks
Title-Subject: [Filtered] Looking for a CL detector for a Quanta 200
Message: I'm in the process of putting together a Quanta 200 and would love to hang a CL detector (any sort for now) on it. If someone has one they are looking to move, let me know and we'll see if we can work something out. Thanks!
Ken Severin
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Title-Subject: [Filtered] Position Available - Research Fellowship  Monash University
Message: Monash University, in Melbourne, Australia, is seeking a research fellow to conduct high-quality research in the development and application of methods to determine atomic structures using CBED, STEM and related techniques.
Duration: 2 years with possible extension to 3.
Full position description and instructions on how to apply can be found at:
http://www.monash.edu.au/jobs/externaljobs.html Search for Job No. 537419
Enquiries to: Professor Joanne Etheridge Tel.: +61 3 9905 1836 Email: joanne.etheridge-at-monash.edu
Closing Date: 8 September 2015
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==============================Original Headers============================== 15, 41 -- From microscopy.listserver-at-gmail.com Thu Aug 20 18:30:26 2015 15, 41 -- Received: from mail-ig0-f170.google.com (mail-ig0-f170.google.com [209.85.213.170]) 15, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7KNUQXR017403 15, 41 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2015 18:30:26 -0500 15, 41 -- Received: by igui7 with SMTP id i7so2771874igu.0 15, 41 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2015 16:30:26 -0700 (PDT) 15, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 41 -- d=gmail.com; s=20120113; 15, 41 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 41 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 41 -- bh=BDf9CKlOUaMJjLPCXEp7E24BWEak1mhPYmbEm5VLtYQ=; 15, 41 -- b=A5gj4Ux+nbShNKEgUhjO4aDlhn3Jd75PNAGDMjh2Kt03dO3yx6WAOgKSb1Z48TIIre 15, 41 -- nXfJgwiqVT277Yb5x3CIUqNwX2i1fVGnm+FD4MPbaINEbUVkNfOTZhT2kJ2Dd8Dlm81M 15, 41 -- Kq9FUfZXoXkJJDnYAOueZDBxcFdEik/hp7HkibYu0za9OjsS+uBeMhP+nRbMNGAhtndq 15, 41 -- ZpzdWFZLhaA+IDB4MxaespCp+QQgOxPIADD/BHFQXRJErAXO9B4p4f7KR9XAmGV3z73c 15, 41 -- G/BqXrvKfP4yJ2bqbRYCAVwjAKZPSYUY6WICi06IsLbIb0C9iF1uRO8+ni747tw+QQv3 15, 41 -- JvXg== 15, 41 -- X-Received: by 10.50.143.2 with SMTP id sa2mr638163igb.72.1440113426209; 15, 41 -- Thu, 20 Aug 2015 16:30:26 -0700 (PDT) 15, 41 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 41 -- by smtp.googlemail.com with ESMTPSA id l14sm4533642ioe.35.2015.08.20.16.30.25 15, 41 -- for {microscopy-at-microscopy.com} 15, 41 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 41 -- Thu, 20 Aug 2015 16:30:25 -0700 (PDT) 15, 41 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 41 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 41 -- {microscopylistserver-noreply-at-microscopy.com} 15, 41 -- Subject: =?UTF-8?Q?viaWWW:Position_Available_-_Research_Fellowship_=c2=96_Mo?= 15, 41 -- =?UTF-8?Q?nash_University?= 15, 41 -- References: {201508200827.t7K8RgmU024560-at-ns.microscopy.com} 15, 41 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 41 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 41 -- X-Forwarded-Message-Id: {201508200827.t7K8RgmU024560-at-ns.microscopy.com} 15, 41 -- Message-ID: {55D66311.8080204-at-microscopy.com} 15, 41 -- Date: Thu, 20 Aug 2015 18:30:25 -0500 15, 41 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 41 -- Gecko/20100101 Thunderbird/38.2.0 15, 41 -- MIME-Version: 1.0 15, 41 -- In-Reply-To: {201508200827.t7K8RgmU024560-at-ns.microscopy.com} 15, 41 -- Content-Type: text/plain; charset=UTF-8; format=flowed 15, 41 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: joseph.mowery-at-ars.usda.gov Name: Joseph Mowery
Organization: USDA ARS SGIL ECMU
Title-Subject: [Filtered] Frozen Insects for TEM
Message: Greetings,
We're interested in processing moths for TEM, but the moths have been stored in a 0F (-18C) freezer for a few weeks or months. Will it still be possible to immerses these moths in glutaraldehyde and process them for conventional TEM? I understand the ultrastructure may not be optimal, but has anyone had success processing frozen insects for TEM?
Thanks, -Joe
Joe Mowery | Biologist Electron and Confocal Microscopy Unit USDA Agricultural Research Service Lab 301-504-9027 | Mobile 817-821-8566 joseph.mowery-at-ars.usda.gov
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==============================Original Headers============================== 15, 40 -- From microscopy.listserver-at-gmail.com Thu Aug 20 18:31:16 2015 15, 40 -- Received: from mail-io0-f174.google.com (mail-io0-f174.google.com [209.85.223.174]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7KNVGMN018752 15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2015 18:31:16 -0500 15, 40 -- Received: by iodv127 with SMTP id v127so64090983iod.3 15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 20 Aug 2015 16:31:15 -0700 (PDT) 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 40 -- d=gmail.com; s=20120113; 15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 40 -- bh=2yMFEVBH7oJyr9zLFxMGeB6iOl40o2zKkW75Diz6WLs=; 15, 40 -- b=Dw0tysu4hTVGqo42gh8kObtEif0+o+yCNRf5IoCTvfwYVed4/GZJhbw1G/U/zP98kj 15, 40 -- vDJ03TXJPLKuepFdbJv9f50fxpa8gWR0Fd5ez6yx41gwCwPX+BOqQrYqDR7825lOXqk4 15, 40 -- 9szwIGCiUtfqt9noP2j4EH5wRW2b/NaZcg8U9K2zOb/EJJNtJMbJSfoam7q8wGeG6ygc 15, 40 -- IVqsZU/7kQBKe58FxzaGZuYPuCEDGAVGegjCTkDhX/Ng0PSjEILux/BfFXQvAOoymQqX 15, 40 -- rQg7PVlfkCvfpvQcOoziW4OvCgfjKfdRv4RTxR8eYK27s6350X2tTqZViC6q/FuihioM 15, 40 -- RC+Q== 15, 40 -- X-Received: by 10.107.136.69 with SMTP id k66mr2056415iod.111.1440113475338; 15, 40 -- Thu, 20 Aug 2015 16:31:15 -0700 (PDT) 15, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 40 -- by smtp.googlemail.com with ESMTPSA id r8sm4547463ioi.10.2015.08.20.16.31.14 15, 40 -- for {microscopy-at-microscopy.com} 15, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 40 -- Thu, 20 Aug 2015 16:31:14 -0700 (PDT) 15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 40 -- {microscopylistserver-noreply-at-microscopy.com} 15, 40 -- Subject: viaWWW:Frozen Insects for TEM 15, 40 -- References: {201508201537.t7KFbEMl002592-at-ns.microscopy.com} 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 40 -- X-Forwarded-Message-Id: {201508201537.t7KFbEMl002592-at-ns.microscopy.com} 15, 40 -- Message-ID: {55D66342.3060203-at-microscopy.com} 15, 40 -- Date: Thu, 20 Aug 2015 18:31:14 -0500 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 40 -- Gecko/20100101 Thunderbird/38.2.0 15, 40 -- MIME-Version: 1.0 15, 40 -- In-Reply-To: {201508201537.t7KFbEMl002592-at-ns.microscopy.com} 15, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: fengxia.liang-at-med.nyu.edu Name: Alice Liang
Organization: NYULMC OCS Microscopy Core
Title-Subject: [Filtered] Biological sample preparation method for element analysis using EDX
Message: Thank you everyone for the quick responses. I learned that the calcium level is important since EDX can only detect high level of the element; EELS might be better for light element analysis. Also it is better to use cryosection since conventional room temperature method might wash out the calcium.
Thanks again, Alice
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Email: oddioeng-at-aol.com Name: j. allen williams, jr
Title-Subject: [Filtered] radon 222 and 220 in uhv system
Message: Greetings,
I am curious to know if anyone has encountered Radon 222 and RDon 220 in your ultra high vacuum systems. All of my valves are metal bellows, vcr fitings with Ni gaskets, large flanges are conflat with copper gaskets.from the fore output of the turbo, to the inlet of the mechanical pump there are qf flanges with viton gaskets. Approximate size of manifold is ~15 ltr. At 60 C, my ion pressure is ~1.4 x 10-8 torr.
With the RGA my Rn 222 peak averages between 3.4 x 10-13 down to about 6.2 x 10-14 in ion current. The 220 peak closely follows. The water vapor peak averages about 5.3 x 10-13 ion current.
Helium 4 leak testing reveals no leaks at ~1.0 x 10-9 sccm-atm. Where is the radon coming from?
Thank you for your time,
J. Allen Williams, Jr
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==============================Original Headers============================== 13, 40 -- From microscopy.listserver-at-gmail.com Fri Aug 21 08:04:03 2015 13, 40 -- Received: from mail-ig0-f175.google.com (mail-ig0-f175.google.com [209.85.213.175]) 13, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7LD430W019578 13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2015 08:04:03 -0500 13, 40 -- Received: by igui7 with SMTP id i7so13454145igu.0 13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2015 06:04:03 -0700 (PDT) 13, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 40 -- d=gmail.com; s=20120113; 13, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 13, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 13, 40 -- bh=zg+Y8y/HiwiWUWwT9QxIcAxeAAMhbXv3GHHe55jxrZg=; 13, 40 -- b=vsNuy9uRAaIyhHi5+GeDXb3FIaBKGtZpS8kjVbmdh7CHwbxM1Uco2HLNMvymTJ1GXG 13, 40 -- mWXV3diTNvb8aLIj4sBkDf6mFRh7zYtrHp0Ljs+Lt1HnSuxP7nha5f6AEIlaaAW0UbbN 13, 40 -- J/nHqh6Tpd0VpChdbokD51X06kAG7A+cUB3RCDyF2vv/SP9qlsScd2C8v2kt/tm7ZSo0 13, 40 -- ABfNRkVyw2ZWNWGMzjEf3ySjylwfGd/zHhrijP8B/LWXnB79Zaapk8sGDrwKk0Wl15yZ 13, 40 -- 0N/Ks51z71jjvZ+OofF0HP1dil3QKrKDpgwtmyR+n9qHnAwgsvCYUImdUqB6S5tlGHVe 13, 40 -- /9Cw== 13, 40 -- X-Received: by 10.50.117.98 with SMTP id kd2mr2689360igb.78.1440162243107; 13, 40 -- Fri, 21 Aug 2015 06:04:03 -0700 (PDT) 13, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 13, 40 -- by smtp.googlemail.com with ESMTPSA id 85sm5919681iot.6.2015.08.21.06.04.02 13, 40 -- for {microscopy-at-microscopy.com} 13, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 40 -- Fri, 21 Aug 2015 06:04:02 -0700 (PDT) 13, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 13, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 13, 40 -- {microscopylistserver-noreply-at-microscopy.com} 13, 40 -- Subject: viaWWW:radon 222 and 220 in uhv system 13, 40 -- References: {201508210144.t7L1iIWj006320-at-ns.microscopy.com} 13, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 40 -- X-Forwarded-Message-Id: {201508210144.t7L1iIWj006320-at-ns.microscopy.com} 13, 40 -- Message-ID: {55D721C1.1070002-at-microscopy.com} 13, 40 -- Date: Fri, 21 Aug 2015 08:04:01 -0500 13, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 13, 40 -- Gecko/20100101 Thunderbird/38.2.0 13, 40 -- MIME-Version: 1.0 13, 40 -- In-Reply-To: {201508210144.t7L1iIWj006320-at-ns.microscopy.com} 13, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
} On Aug 21, 2015, at 6:25 AM, microscopy.listserver-at-gmail.com wrote: } } Email: oddioeng-at-aol.com } Name: j. allen williams, jr } } Title-Subject: [Filtered] radon 222 and 220 in uhv system } } Message: Greetings, } } I am curious to know if anyone has encountered Radon 222 and RDon 220 in your ultra high vacuum } systems. All of my valves are metal bellows, vcr fitings with Ni gaskets, large flanges are conflat } with copper gaskets.from the fore output of the turbo, to the inlet of the mechanical pump there are } qf flanges with viton gaskets. Approximate size of manifold is ~15 ltr. At 60 C, my ion pressure is } ~1.4 x 10-8 torr. } } With the RGA my Rn 222 peak averages between 3.4 x 10-13 down to about 6.2 x 10-14 in ion current. } The 220 peak closely follows. The water vapor peak averages about 5.3 x 10-13 ion current. } } Helium 4 leak testing reveals no leaks at ~1.0 x 10-9 sccm-atm. } Where is the radon coming from? } } Thank you for your time, } } J. Allen Williams, Jr } Dear Allen, The source of the radon could be the soil, depending where you are. For example, there is a formation called the Reading Prong in PA and NY that is very high in Rn, so much so that installing a heat pump system, which requires that the building is well sealed, leads to dangerous levels. Since Ems are typically in sub-basements, one would expect the highest Rn concentrations there. In fact, unless you have a few grams of radium lying around, the soil is certainly the source. Yours, Bill
==============================Original Headers============================== 6, 33 -- From wtivol-at-sbcglobal.net Fri Aug 21 19:46:12 2015 6, 33 -- Received: from nm22-vm2.access.bullet.mail.bf1.yahoo.com (nm22-vm2.access.bullet.mail.bf1.yahoo.com [216.109.115.145]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7M0k9eo025344 6, 33 -- for {microscopy-at-microscopy.com} ; Fri, 21 Aug 2015 19:46:11 -0500 6, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1440204369; bh=NJ73FGAKmHV7X/NwoWeVZ8aA1ktdft9ba1V0AzsyxXc=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=FsVled0abRe7S2QvYCkij1Tay5WjFZ6vGA5IA9tnOXyiDZbrmo0gxRipw0XxHMGCsKRQ3cm/5eM+zfwS/xd4YXzSdT+d6wy+kV4Lho5J5pOCeC66q8SYiIrVziDVpyUuGT9HALOqdWAa4ubFTgbxaqrFtl2t5mhlFNvJMEAa+jJsZwfRhicJk7KbtaJ+SM1sEqOilycsEvtgOQEF8qsPcSGp3tgK3dHqxzrxwvFkg4wISiyhYW/Mod73k16W8FLLPORDR6W2dz2jqk8H3QYTfLYWEnhuPMrRavxfdUCrwqy5zP2ZjDYkT9+FZL+kc41A2DxXJTNeuWmzX75t100TGA== 6, 33 -- Received: from [66.196.81.164] by nm22.access.bullet.mail.bf1.yahoo.com with NNFMP; 22 Aug 2015 00:46:09 -0000 6, 33 -- Received: from [98.139.221.250] by tm10.access.bullet.mail.bf1.yahoo.com with NNFMP; 22 Aug 2015 00:46:09 -0000 6, 33 -- Received: from [127.0.0.1] by smtp120.sbc.mail.bf1.yahoo.com with NNFMP; 22 Aug 2015 00:46:09 -0000 6, 33 -- X-Yahoo-Newman-Id: 74489.86480.bm-at-smtp120.sbc.mail.bf1.yahoo.com 6, 33 -- X-Yahoo-Newman-Property: ymail-3 6, 33 -- X-YMail-OSG: 3DJjh70VM1m2Q_DfEaFI7mypxeZ.6Rtoir45Gvk2zCuz6sg 6, 33 -- TC4pOorVKgXntZXoy2FkH3P6uCCMfi9WVi60TuP..074gASSwIu7th3X4.MM 6, 33 -- fYEPMTemIcVSLjTsgevEKSzipKM41ykYbz2UcFmNgxgANU650mVFoGPs3Rnd 6, 33 -- voBGYxhe__NXvRTPw9muedY6L9yGVvRdMJGTkYHrT58STW6Q7l7f.5G.WW9s 6, 33 -- HFJGmwLta5NjVyRwhEKBgamvcHShD0TC3Z9sDoqcrp.JfInbsIoXmIFIQq8h 6, 33 -- aoVAq_vl6B4dN6mxKakn55xrh.4ppIgf9zA3v4wniB80KwrUGpzEWLi2eAYl 6, 33 -- jjyOOcJmzpwcMJFd.QoNbFbbzpgxFneNI_yG55Pht7jArCex2Xtskar5lsy7 6, 33 -- KPZFws5TjHgj.RjIl8ImtiOEq1zgJClS30fIIBLdroZKksst4b5Q6IwlIpGm 6, 33 -- uvaclj1.87Gj.zsSF6ztgPNxIOf15gr1fm9Zde.fz3BTpTPPK6CKxEP4pSbY 6, 33 -- dNdFtZ5eyKnVyk.NFVtrTscJVNkedhhcRgMoJgkocZlq4LDVZr_nM 6, 33 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 33 -- Content-Type: text/plain; charset=us-ascii 6, 33 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 6, 33 -- Subject: Re: [Microscopy] viaWWW:radon 222 and 220 in uhv system 6, 33 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 33 -- In-Reply-To: {201508211325.t7LDPvx3001967-at-ns.microscopy.com} 6, 33 -- Date: Fri, 21 Aug 2015 17:46:05 -0700 6, 33 -- Message-Id: {1FEC8859-AD9C-4950-B8D5-5777F589DD7B-at-sbcglobal.net} 6, 33 -- References: {201508211325.t7LDPvx3001967-at-ns.microscopy.com} 6, 33 -- To: microscopy-at-microscopy.com 6, 33 -- X-Mailer: Apple Mail (2.2104) 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7M0k9eo025344 ==============================End of - Headers==============================
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Email: s.s.doak-at-lboro.ac.uk Name: Scott Doak
Organization: Materials Department, Loughborough University, UK
Title-Subject: [Filtered] Position Open:Research Development Officer
Message: Closing Date: 14 September 2015 The post is a full time, permanent role on grade 7 (Ł38,511 - Ł45,954). Informal enquiries should be made to Scott Doak by email at: S.S.Doak-at-lboro.ac.uk or by telephone on +44 (0)1509 223314.
A vacancy has arisen for a Research Development Officer in Loughborough Materials Characterisation Centre (LMCC), in the Department of Materials at Loughborough University. This would suit an Electron Microscopist with a background in Materials Science. The LMCC provides a high quality materials characterisation facility (including electron and optical microscopy, x-ray diffraction, thermal analysis and surface analysis) to support research within Loughborough University, the wider academic community and for industrial clients. This specialised role will play a vital part in representing the LMCC to external organisations, delivering and managing existing projects and developing new projects from inception stage. The post holder will be a technical specialist, with hands on experience of advanced microstructural characterisation techniques, such as SEM, FIB, EDS, EBSD and TEM. The ideal applicant will also have a track record of undertaking research involving relevant instrumentation and experience of writing or contributing to successful research grant applications.
Full position description and instructions on how to apply can be found at: https://vacancies.lboro.ac.uk/tlive_webrecruitment/wrd/run/ETREC107GF.open?VACANCY_ID%3d6376885YIl%1B&WVID=5913100PrZ&LANG=USA
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==============================Original Headers============================== 15, 41 -- From microscopy.listserver-at-gmail.com Mon Aug 24 08:58:19 2015 15, 41 -- Received: from mail-io0-f182.google.com (mail-io0-f182.google.com [209.85.223.182]) 15, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7ODwJvg019901 15, 41 -- for {microscopy-at-microscopy.com} ; Mon, 24 Aug 2015 08:58:19 -0500 15, 41 -- Received: by iodv127 with SMTP id v127so148987017iod.3 15, 41 -- for {microscopy-at-microscopy.com} ; Mon, 24 Aug 2015 06:58:19 -0700 (PDT) 15, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 41 -- d=gmail.com; s=20120113; 15, 41 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 41 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 41 -- bh=RffAivI/4mmr4K6WfEIIy1GOmRnpg6wa0Dvb1YEEL2g=; 15, 41 -- b=OtRrHMku0DmdtAztw3mRwH8KoGRq8UhoxAoXUryZxCP4r7JpJgT+4KzFPGWpkNChoT 15, 41 -- oZ/3XPzWlTlJ9xgElOqRYOc397NY5uvE5CrNmQntISpnFgj3H1inn3yB6ftoG1EZpchK 15, 41 -- 6wSsp6z+UPWbkuXXZY5+2mO7UjOze1XCzhK/yNokAuGy5TQCL1kIwKMRTR467/Gz0DXN 15, 41 -- 89TYznUZjVUi9YsMv3fN2wQ/G4Kgg9XC1CCuuwflecvNRExS1EzZTEqNl0g8z2eeYYO2 15, 41 -- xkze+J/VxFcgime9QuFDViAegEFrQ8en+SzizgEzFS5W5PrZtiH8LVIh2ggO22oNFD6+ 15, 41 -- uRTw== 15, 41 -- X-Received: by 10.107.19.202 with SMTP id 71mr17297992iot.94.1440424699097; 15, 41 -- Mon, 24 Aug 2015 06:58:19 -0700 (PDT) 15, 41 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 41 -- by smtp.googlemail.com with ESMTPSA id c64sm7372865ioc.13.2015.08.24.06.58.18 15, 41 -- for {microscopy-at-microscopy.com} 15, 41 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 41 -- Mon, 24 Aug 2015 06:58:18 -0700 (PDT) 15, 41 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 41 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 41 -- {microscopylistserver-noreply-at-microscopy.com} 15, 41 -- Subject: viaWWW:Position Open:Research Development Officer Loughborough 15, 41 -- University 15, 41 -- References: {201508241121.t7OBLQhD016594-at-ns.microscopy.com} 15, 41 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 41 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 41 -- X-Forwarded-Message-Id: {201508241121.t7OBLQhD016594-at-ns.microscopy.com} 15, 41 -- Message-ID: {55DB22F9.3020908-at-microscopy.com} 15, 41 -- Date: Mon, 24 Aug 2015 08:58:17 -0500 15, 41 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 41 -- Gecko/20100101 Thunderbird/38.2.0 15, 41 -- MIME-Version: 1.0 15, 41 -- In-Reply-To: {201508241121.t7OBLQhD016594-at-ns.microscopy.com} 15, 41 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 41 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: H.Wu2-at-lboro.ac.uk Name: Dr H Wu
Organization: Materials Department, Loughborough University, UK
Title-Subject: [Filtered] Position open: Research Associate in Nuclear Graphite
Message: Understanding and Improving Graphite for Advanced Nuclear Fission (UNIGRAF)
Fixed term for 36 months. Closing date: 8th September 2015 For full detail follow the link: http://www.jobs.ac.uk/job/ALT703/research-associate-in-nuclear-graphite/
Required to undertake experimental research on structure from atomic- to micro-scale of iso-graphite for advanced nuclear reactors. The project aims to improve the capability of nuclear graphite through a better understanding of irradiation-induced changes in structure and their impact on mechanical/physical behaviour. The post is part of an EPSRC funded project "UNIGRAF" in partnership with Oxford and Bristol University, and multi supporters in USA, China and Germany.
Based at Loughborough the post holder will have strong collaborations with scientists/engineers from all partners, and focus on structure characterisation before and after irradiation using HRTEM, EELS, FIB and other techniques. You will have opportunity to access structural characterisation facilities in Oak Ridge National Laboratory, Oxford, and EPSRC National Facility, and multi overseas travel is expected. A good honours degree (2.1 minimum) and a PhD or equivalent in Materials Science, Physics, or another relevant discipline and experience in materials structural characterisation techniques is essential. Research in graphite/graphene materials and knowledge in nuclear materials is desirable.
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==============================Original Headers============================== 15, 40 -- From microscopy.listserver-at-gmail.com Mon Aug 24 08:59:16 2015 15, 40 -- Received: from mail-ig0-f181.google.com (mail-ig0-f181.google.com [209.85.213.181]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7ODxGdf020335 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 24 Aug 2015 08:59:16 -0500 15, 40 -- Received: by igcse8 with SMTP id se8so47577569igc.1 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 24 Aug 2015 06:59:16 -0700 (PDT) 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 40 -- d=gmail.com; s=20120113; 15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 40 -- bh=UDXGNEPiniqdH2JNoHPUumRSd8vBMjmeOFtz1PKqcrQ=; 15, 40 -- b=ZqWDHWW4zsAkCA2XF/Q3XokDGtXEO3+mQtL1Rjaz/9TP6Jzsesi3S/pChXWu2cGejh 15, 40 -- FIAv3grtpHjZNhXx7xL2seYeiJX2HRTaCdEvZF66qy1t+g0cCXz/ZHDAm+mjq78aDD0Y 15, 40 -- QfUb0lRgalTk//cR5k+mDDzMNpeFXVv61xai75ThgEnY0+VHqvmsfP9yO4+YI2TEOzFC 15, 40 -- FLgZUb6NAr+8Dwo1+i0ktj/lSyhgh9p9l+4+sYy/NeI0IMEgOXVn0mQWa91DRk/LvJQM 15, 40 -- EkDRcUTCrVKt0X7rxKyOPzar2HdaWwwhC7rqrpSKyRQYYVS/easmOiLxZSelDz5H31wO 15, 40 -- 9ZOw== 15, 40 -- X-Received: by 10.50.61.195 with SMTP id s3mr15134651igr.62.1440424756203; 15, 40 -- Mon, 24 Aug 2015 06:59:16 -0700 (PDT) 15, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 40 -- by smtp.googlemail.com with ESMTPSA id d143sm7533489ioe.34.2015.08.24.06.59.15 15, 40 -- for {microscopy-at-microscopy.com} 15, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 40 -- Mon, 24 Aug 2015 06:59:15 -0700 (PDT) 15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 40 -- {microscopylistserver-noreply-at-microscopy.com} 15, 40 -- Subject: viaWWW:Position open: Research Associate in Nuclear Graphite 15, 40 -- References: {201508241133.t7OBXmWT016730-at-ns.microscopy.com} 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 40 -- X-Forwarded-Message-Id: {201508241133.t7OBXmWT016730-at-ns.microscopy.com} 15, 40 -- Message-ID: {55DB2333.2020909-at-microscopy.com} 15, 40 -- Date: Mon, 24 Aug 2015 08:59:15 -0500 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 40 -- Gecko/20100101 Thunderbird/38.2.0 15, 40 -- MIME-Version: 1.0 15, 40 -- In-Reply-To: {201508241133.t7OBXmWT016730-at-ns.microscopy.com} 15, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: mlibbee-at-gmail.com Name: Marissa
Organization: NCEM/MF
Title-Subject: [Filtered] cryo- transfer systems
Message: If anyone owns or has experience with the Quorum, Gatan or Leica cryo- transfer systems and has a willingness to offer some advice and feedback, please contact me offline.
Thank you,
Marissa Libbee 510-495-2308 mlibbee-at-gmail.com
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Dear Listers,
A researcher here at UNMC is collecting mitochondria and synaptosome fractions via differential ultracentrifugation. They would like to know which buffer is best to use in the fixative,0.1M Sodium Cacodylate or 0.1M Sorensen's Phosphate Buffer. The aldehyde percentages are 2% glutaraldehyde and 2% paraformaldehyde. All advice is appreciated, thank you.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 39 -- From tbargar-at-unmc.edu Wed Aug 26 16:34:38 2015 7, 39 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7QLYcJK032300 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:34:38 -0500 7, 39 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 39 -- by Outbound.unmc.edu (Proprietary) with SMTP id 7C47B5BCDEB 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:24:06 -0500 (CDT) 7, 39 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 39 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 39 -- (No client certificate requested) 7, 39 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id ADD385BCC8E 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:24:05 -0500 (CDT) 7, 39 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 39 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 39 -- 14.03.0235.001; Wed, 26 Aug 2015 16:34:37 -0500 7, 39 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 39 -- Subject: Differential ultracentrifugation fractions, best buffer to use in 7, 39 -- fixation 7, 39 -- Thread-Topic: Differential ultracentrifugation fractions, best buffer to use 7, 39 -- in fixation 7, 39 -- Thread-Index: AdDgRoQW9hgI86NaQ7mI19ksgb14iQ== 7, 39 -- Date: Wed, 26 Aug 2015 21:34:36 +0000 7, 39 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0747337ED-at-UNMCEX2.unmcresforest.org} 7, 39 -- Accept-Language: en-US 7, 39 -- Content-Language: en-US 7, 39 -- X-MS-Has-Attach: 7, 39 -- X-MS-TNEF-Correlator: 7, 39 -- x-originating-ip: [10.8.64.15] 7, 39 -- Content-Type: text/plain; charset="us-ascii" 7, 39 -- MIME-Version: 1.0 7, 39 -- X-VPM-MSG-ID: e07ad4ff-83f7-4d4f-9dc4-829a6926d8dc 7, 39 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 39 -- X-VPM-GROUP-ID: 7830dee5-1ccd-450f-9147-5f5b6a287f2a 7, 39 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 39 -- X-VPM-CERT-FLAG: 0 7, 39 -- X-VPM-IS-HYBRID: 0 7, 39 -- Content-Transfer-Encoding: 8bit 7, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7QLYcJK032300 ==============================End of - Headers==============================
A researcher here at UNMC is collecting mitochondria and synaptosome fractions via differential ultracentrifugation. They would like to know which buffer is best to use in the fixative,0.1M Sodium Cacodylate or 0.1M Sorensen's Phosphate Buffer. The aldehyde percentages are 2% glutaraldehyde and 2% paraformaldehyde. All advice is appreciated, thank you.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 7, 39 -- From tbargar-at-unmc.edu Wed Aug 26 16:51:44 2015 7, 39 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7QLpi42015924 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:51:44 -0500 7, 39 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 39 -- by Outbound.unmc.edu (Proprietary) with SMTP id 7C47B5BCDEB 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:24:06 -0500 (CDT) 7, 39 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 39 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 39 -- (No client certificate requested) 7, 39 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id ADD385BCC8E 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:24:05 -0500 (CDT) 7, 39 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 39 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 39 -- 14.03.0235.001; Wed, 26 Aug 2015 16:34:37 -0500 7, 39 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 39 -- Subject: Differential ultracentrifugation fractions, best buffer to use in 7, 39 -- fixation 7, 39 -- Thread-Topic: Differential ultracentrifugation fractions, best buffer to use 7, 39 -- in fixation 7, 39 -- Thread-Index: AdDgRoQW9hgI86NaQ7mI19ksgb14iQ== 7, 39 -- Date: Wed, 26 Aug 2015 21:34:36 +0000 7, 39 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0747337ED-at-UNMCEX2.unmcresforest.org} 7, 39 -- Accept-Language: en-US 7, 39 -- Content-Language: en-US 7, 39 -- X-MS-Has-Attach: 7, 39 -- X-MS-TNEF-Correlator: 7, 39 -- x-originating-ip: [10.8.64.15] 7, 39 -- Content-Type: text/plain; charset="us-ascii" 7, 39 -- MIME-Version: 1.0 7, 39 -- X-VPM-MSG-ID: e07ad4ff-83f7-4d4f-9dc4-829a6926d8dc 7, 39 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 39 -- X-VPM-GROUP-ID: 7830dee5-1ccd-450f-9147-5f5b6a287f2a 7, 39 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 39 -- X-VPM-CERT-FLAG: 0 7, 39 -- X-VPM-IS-HYBRID: 0 7, 39 -- Content-Transfer-Encoding: 8bit 7, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7QLpi42015924 ==============================End of - Headers==============================
Phosphate buffer will cause calcium to precipitate out and form noticeable dark spots in the TEM - especially in mitochondria. Cacodylate buffer will avoid that but so will one of the more modern generation buffers such as HEPES or PIPES. Why deal with the toxicity and increased environmental burden of using an arsenic-based buffer? I admit buffers can alter the preservation in subtle ways but a priori there is no reason to assume cacodylate is superior to HEPES or PIPES. I use HEPES at pH 7.4 for my fixations.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Wednesday, August 26, 2015 4:52 PM To: Phillips, Thomas E. {PhillipsT-at-missouri.edu}
Dear Listers,
A researcher here at UNMC is collecting mitochondria and synaptosome fractions via differential ultracentrifugation. They would like to know which buffer is best to use in the fixative,0.1M Sodium Cacodylate or 0.1M Sorensen's Phosphate Buffer. The aldehyde percentages are 2% glutaraldehyde and 2% paraformaldehyde. All advice is appreciated, thank you.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 7, 39 -- From tbargar-at-unmc.edu Wed Aug 26 16:51:44 2015 7, 39 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7QLpi42015924 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:51:44 -0500 7, 39 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 39 -- by Outbound.unmc.edu (Proprietary) with SMTP id 7C47B5BCDEB 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:24:06 -0500 (CDT) 7, 39 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 39 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 39 -- (No client certificate requested) 7, 39 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id ADD385BCC8E 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:24:05 -0500 (CDT) 7, 39 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 39 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 39 -- 14.03.0235.001; Wed, 26 Aug 2015 16:34:37 -0500 7, 39 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 39 -- Subject: Differential ultracentrifugation fractions, best buffer to use in 7, 39 -- fixation 7, 39 -- Thread-Topic: Differential ultracentrifugation fractions, best buffer to use 7, 39 -- in fixation 7, 39 -- Thread-Index: AdDgRoQW9hgI86NaQ7mI19ksgb14iQ== 7, 39 -- Date: Wed, 26 Aug 2015 21:34:36 +0000 7, 39 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0747337ED-at-UNMCEX2.unmcresforest.org} 7, 39 -- Accept-Language: en-US 7, 39 -- Content-Language: en-US 7, 39 -- X-MS-Has-Attach: 7, 39 -- X-MS-TNEF-Correlator: 7, 39 -- x-originating-ip: [10.8.64.15] 7, 39 -- Content-Type: text/plain; charset="us-ascii" 7, 39 -- MIME-Version: 1.0 7, 39 -- X-VPM-MSG-ID: e07ad4ff-83f7-4d4f-9dc4-829a6926d8dc 7, 39 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 39 -- X-VPM-GROUP-ID: 7830dee5-1ccd-450f-9147-5f5b6a287f2a 7, 39 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 39 -- X-VPM-CERT-FLAG: 0 7, 39 -- X-VPM-IS-HYBRID: 0 7, 39 -- Content-Transfer-Encoding: 8bit 7, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7QLpi42015924 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 34 -- From PhillipsT-at-missouri.edu Wed Aug 26 17:35:59 2015 18, 34 -- Received: from um-nip4-missouri-out.um.umsystem.edu (um-nip4-missouri-out.um.umsystem.edu [198.209.49.177]) 18, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7QMZx5i009721 18, 34 -- for {microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 17:35:59 -0500 18, 34 -- X-IronPort-Anti-Spam-Filtered: true 18, 34 -- X-IronPort-Anti-Spam-Result: A2AfBQAxPt5V/9CeoM9aAw6DDVRpBr90hXsCgTY8EAEBAQEBAQGBCoQjAQEBBDoUNwICAgEIEQMBAQELAhIJBxYbARQJCAIEAQkJCBUEiA0NxDQBhGgBAQEBAQEBAQEBAQEBAQEBAQEBAQEXBItXgm0JAYFiBgUEEgsHBQIEDIMGgRQFhymOEAGFBYk2RoNslGImgg00gQA+cQEBgUaBBQEBAQ 18, 34 -- X-IPAS-Result: A2AfBQAxPt5V/9CeoM9aAw6DDVRpBr90hXsCgTY8EAEBAQEBAQGBCoQjAQEBBDoUNwICAgEIEQMBAQELAhIJBxYbARQJCAIEAQkJCBUEiA0NxDQBhGgBAQEBAQEBAQEBAQEBAQEBAQEBAQEXBItXgm0JAYFiBgUEEgsHBQIEDIMGgRQFhymOEAGFBYk2RoNslGImgg00gQA+cQEBgUaBBQEBAQ 18, 34 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) 18, 34 -- by um-nip4-exch-relay.um.umsystem.edu with ESMTP; 26 Aug 2015 17:35:58 -0500 18, 34 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.122]) by 18, 34 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi id 14.03.0248.002; 18, 34 -- Wed, 26 Aug 2015 17:35:58 -0500 18, 34 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 18, 34 -- To: "tbargar-at-unmc.edu" {tbargar-at-unmc.edu} , 18, 34 -- "'microscopy-at-microscopy.com'" 18, 34 -- {microscopy-at-microscopy.com} 18, 34 -- Subject: RE: [Microscopy] Differential ultracentrifugation fractions, best 18, 34 -- buffer to use in 18, 34 -- Thread-Topic: [Microscopy] Differential ultracentrifugation fractions, best 18, 34 -- buffer to use in 18, 34 -- Thread-Index: AQHQ4EmBJqDIMynwxUK7U/YSmygkcJ4e3TPg 18, 34 -- Date: Wed, 26 Aug 2015 22:35:58 +0000 18, 34 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DE722C05F-at-UM-MBX-T02.um.umsystem.edu} 18, 34 -- References: {201508262152.t7QLqSlx017409-at-ns.microscopy.com} 18, 34 -- In-Reply-To: {201508262152.t7QLqSlx017409-at-ns.microscopy.com} 18, 34 -- Accept-Language: en-US 18, 34 -- Content-Language: en-US 18, 34 -- X-MS-Has-Attach: 18, 34 -- X-MS-TNEF-Correlator: 18, 34 -- x-originating-ip: [128.206.81.115] 18, 34 -- Content-Type: text/plain; charset="us-ascii" 18, 34 -- MIME-Version: 1.0 18, 34 -- Content-Transfer-Encoding: 8bit 18, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7QMZx5i009721 ==============================End of - Headers==============================
I can only second that piece of phillipsian wisdom. :-)
Stephane
-------------------------------------------- On Thu, 8/27/15, PhillipsT-at-missouri.edu {PhillipsT-at-missouri.edu} wrote:
Subject: [Microscopy] RE: Differential ultracentrifugation fractions, best To: nizets2-at-yahoo.com Date: Thursday, August 27, 2015, 12:40 AM
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Phosphate buffer will cause calcium to precipitate out and form noticeable dark spots in the TEM - especially in mitochondria. Cacodylate buffer will avoid that but so will one of the more modern generation buffers such as HEPES or PIPES. Why deal with the toxicity and increased environmental burden of using an arsenic-based buffer? I admit buffers can alter the preservation in subtle ways but a priori there is no reason to assume cacodylate is superior to HEPES or PIPES. I use HEPES at pH 7.4 for my fixations.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Wednesday, August 26, 2015 4:52 PM To: Phillips, Thomas E. {PhillipsT-at-missouri.edu} Subject: [Microscopy] Differential ultracentrifugation fractions, best buffer to use in
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Dear Listers,
A researcher here at UNMC is collecting mitochondria and synaptosome fractions via differential ultracentrifugation. They would like to know which buffer is best to use in the fixative,0.1M Sodium Cacodylate or 0.1M Sorensen's Phosphate Buffer. The aldehyde percentages are 2% glutaraldehyde and 2% paraformaldehyde. All advice is appreciated, thank you.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 39 -- From tbargar-at-unmc.edu Wed Aug 26 16:51:44 2015 7, 39 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7QLpi42015924 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:51:44 -0500 7, 39 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 39 -- by Outbound.unmc.edu (Proprietary) with SMTP id 7C47B5BCDEB 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:24:06 -0500 (CDT) 7, 39 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 39 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 39 -- (No client certificate requested) 7, 39 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id ADD385BCC8E 7, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Aug 2015 16:24:05 -0500 (CDT) 7, 39 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 39 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 39 -- 14.03.0235.001; Wed, 26 Aug 2015 16:34:37 -0500 7, 39 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 39 -- Subject: Differential ultracentrifugation fractions, best buffer to use in 7, 39 -- fixation 7, 39 -- Thread-Topic: Differential ultracentrifugation fractions, best buffer to use 7, 39 -- in fixation 7, 39 -- Thread-Index: AdDgRoQW9hgI86NaQ7mI19ksgb14iQ== 7, 39 -- Date: Wed, 26 Aug 2015 21:34:36 +0000 7, 39 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0747337ED-at-UNMCEX2.unmcresforest.org} 7, 39 -- Accept-Language: en-US 7, 39 -- Content-Language: en-US 7, 39 -- X-MS-Has-Attach: 7, 39 -- X-MS-TNEF-Correlator: 7, 39 -- x-originating-ip: [10.8.64.15] 7, 39 -- Content-Type: text/plain; charset="us-ascii" 7, 39 -- MIME-Version: 1.0 7, 39 -- X-VPM-MSG-ID: e07ad4ff-83f7-4d4f-9dc4-829a6926d8dc 7, 39 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 39 -- X-VPM-GROUP-ID: 7830dee5-1ccd-450f-9147-5f5b6a287f2a 7, 39 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 39 -- X-VPM-CERT-FLAG: 0 7, 39 -- X-VPM-IS-HYBRID: 0 7, 39 -- Content-Transfer-Encoding: 8bit 7, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7QLpi42015924 ==============================End of - Headers==============================
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Summary (you don't need the details to take a decision): "not optimal" is mildly put. Leaving biological material unfixed at -18°C (it is probably the worst method to preserver biological material) and expecting to see anything meaningful in TEM is not realistic. If you want to use TEM, you are interested in fine morphology right? Well you won't get fine morphological information this way.
Details (if you want to know why): 1) Upon freezing and especially slowly freezing like it happens when you put a specimen at -18°C, the water in the tissue and cells crystallizes. Nice spiky crystals. The crystals don't care about the biological structures (like membranes), they grow through them, perforating them. Upon thawing, the membrane are literally cut in pieces, leaving you with a biological soup with very few original morphological properties. 2) The cellular enzymes have had plenty of time to do whatever job they have to do 3) Big osmotic issues. Slowly freezing produces extreme osmotic forces because while some water freezes the remaining water which is in liquid state sees its salt concentration dramatically increase
Hope you can convince your colleagues to invest time and money in something more useful (like reading a book about the usefulness of fixatives).
Stephane
-------------------------------------------- On Fri, 8/21/15, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
Subject: [Microscopy] viaWWW:Frozen Insects for TEM To: nizets2-at-yahoo.com Date: Friday, August 21, 2015, 1:45 AM
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Email: joseph.mowery-at-ars.usda.gov Name: Joseph Mowery
Organization: USDA ARS SGIL ECMU
Title-Subject: [Filtered] Frozen Insects for TEM
Message: Greetings,
We're interested in processing moths for TEM, but the moths have been stored in a 0F (-18C) freezer for a few weeks or months. Will it still be possible to immerses these moths in glutaraldehyde and process them for conventional TEM? I understand the ultrastructure may not be optimal, but has anyone had success processing frozen insects for TEM?
Thanks, -Joe
Joe Mowery | Biologist Electron and Confocal Microscopy Unit USDA Agricultural Research Service Lab 301-504-9027 | Mobile 817-821-8566 joseph.mowery-at-ars.usda.gov
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Email: rhsia-at-umaryland.edu Name: Ru-ching Hsia
Organization: University of Maryland, Baltimore
Title-Subject: [Filtered] Course annoucement: Basic-ultramicrotomy mini-course, October 26th and 27th, 2015
Message: Dear Colleagues,
We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a two day basic-ultramicrotomy mini-course on October 26th and 27th, 2015. This course is for beginner or novice who wish to learn or sharpen their room temperature ultramicrotomy techniques for sectioning resin embedded biological material. No prior experience is required. The course will include lectures, demonstrations and hands on practice. Below are the topics that will be covered in the course  Mechanism and functionality of an ultramicrotome.  Use and care of diamond knives.  Glass knives and making of glass knives.  Ultramicrotomy of biological specimen workflow and general practice.  Ultramicrotomy clinics, common problems and troubleshooting.
More information regarding the course and registration can be found in our website
Please email coreimaging-at-umaryland.edu for any inquiries.
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Email: gul417-at-mail.usask.ca Name: Guosheng Liu
Organization: U of Saskatchewan
Title-Subject: [Filtered] CM10 TEM: beam shines like a dot but occasionally spreads normal shape.
Message: Hello all,
Our Philips CM10 TEM got a beam issue---it looked like in a dark field mode, concentrated as a tiny point and could not be spread out. But suddenly it might be normal for a few seconds to less than a minute without touching anywhere, then went back to point beam again.
The ODP/IGP vacuum readings are not ideal but seems working before. Anything else could be the cause of this problem? Where/how to check first?
Your help and suggestion are greatly appreciated.
Thank you in advance.
Guosheng
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Helo Guosheng, It might be the board regulating current for C2 condenser. You can check the current passing through C2 in this way: Go to PARAMETERS page on information CRT and press DISPLAY CURRENTS. Then you can monitor second condenser (C2) current. Go to SINGLE mode, select C2 and look what happens when you turn the INTENSITY button back and forth. If there is no change in current level for C2 then the trouble might be in the current regulating board.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Thu, 27 Aug 2015 17:56:42 -0500, microscopy.listserver-at-gmail.com wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: gul417-at-mail.usask.ca } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both gul417-at-mail.usask.ca as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: gul417-at-mail.usask.ca } Name: Guosheng Liu } } Organization: U of Saskatchewan } } Title-Subject: [Filtered] CM10 TEM: beam shines like a dot but } occasionally spreads normal shape. } } Message: Hello all, } } Our Philips CM10 TEM got a beam issue---it looked like in a dark } field mode, concentrated as a tiny point and could not be spread out. } But suddenly it might be normal for a few seconds to less than a } minute without touching anywhere, then went back to point beam again. } } The ODP/IGP vacuum readings are not ideal but seems working before. } Anything else could be the cause of this problem? Where/how to check } first? } } Your help and suggestion are greatly appreciated. } } Thank you in advance. } } Guosheng } } Login Host: 128.233.13.22 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original } Headers============================== 17, 41 -- From } microscopy.listserver-at-gmail.com Thu Aug 27 17:33:46 2015 17, 41 -- } Received: from mail-qk0-f172.google.com (mail-qk0-f172.google.com } [209.85.220.172]) 17, 41 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id t7RMXjaQ019485 17, 41 -- } for {microscopy-at-microscopy.com} ; Thu, 27 Aug 2015 17:33:45 } -0500 17, 41 -- Received: by qkda128 with SMTP id a128so19182830qkd.3 } 17, 41 -- for {microscopy-at-microscopy.com} ; Thu, 27 Aug 2015 } 15:33:45 -0700 (PDT) 17, 41 -- DKIM-Signature: v=1; a=rsa-sha256; } c=relaxed/relaxed; 17, 41 -- d=gmail.com; s=20120113; 17, 41 } -- } h=from:subject:references:to:reply-to:message-id:date:user-agent 17, } 41 } -- :mime-version:in-reply-to:content-type:content-transfer-encoding; } 17, 41 -- bh=cIJieG2D1Qo522FtGFVsfsEUrRIe/AhzZFuZDxRO4r0=; } 17, 41 -- } b=si2eBPUM6mxlakxb/z2rhehxaLPh6I4EwYVbr871Ms45dZMHPPVc7L3pcxL/nunYse } 17, 41 -- } Ck6AE5GMZS/6ATSAYOH0Ae+H7rVtSlqSYIbfIFePWXVWY7TstkBOAke7+eEVBh1XAkM6 } 17, 41 -- } Xtw1dZnaybAc6LOANv3Tmr1K07k5Jev2XGP9MeqCe9KxCtIKqFkyMwddsFKR6Fo5hAvk } 17, 41 -- } +5j9m7jGMgLfIOY1RcTEjKufh4Cx3Lc7tadlHanXIKTMTr7SF4Vmn11lxK/2E0yIqJfx } 17, 41 } -- /H/WCduXpWpqBjKzeJ9b+4802XydVTLhoOMeRcDxLtMRGJb7vvxieqn7kIl82EZp9e2L } 17, 41 -- h0xw== 17, 41 -- X-Received: by 10.55.198.92 with
Definitely sounds like one or more of the lenses is cutting out (check them all on Parameters page, as Oldrich suggests).
We are having the same issue with our CM10- caused by insufficient water flow through the heatsinks (MRU and LMH) in the lower right of the microscope (if you feel the two hoses at almost ground level to the left of the rotary pump, one of them will be the return with very hot water). The water flows around the column (top and bottom circuits of the column in parallel), then through the MRU and LMH heatsinks, then out of the microscope (the diff. pump water circuit is in parallel with this).
If the microscope is one for an extended period, as well as the MRU/LMH water causing the lenses to cut out, we also have the lower part of the column heating up (felt by hand on the surface). We are going to solve it by looping out the MRU+LMH part of the circuit, and instead feeding it a separate water supply from our chiller circuit.
There is a blow-off option that can help clear this circuit. The connector for this is on the right when you have the back of the microscope off. When you pull out the connector, a switch will turn off the microscope automatically. This quick-coupler is then connected to the inlet to push out water + debris from the lenses, MRU, LMH part of the microscope's cooling circuit. If you do this, make sure you know where the air will go when it is forced out of the return line of the microscope- we have had engineers make a fountain out the top of our chiller. We have a drain we can open.
Good luck,
Ben -- Research Support Manager and Acting IT Manager MRC Brain Network Dynamics Unit, University of Oxford Department of Pharmacology, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271649 {http://www.mrcbndu.ox.ac.uk/}
On 28/08/2015 09:06, "benada-at-biomed.cas.cz" {benada-at-biomed.cas.cz} wrote:
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If the beam is a pinpoint of light, the most likely cause is water flow. You will see on the Lens Current page that all the main lens values will be close to 0. You should have water flow gauges that are located near the mechanical pump. These have magnetic floats in them and if the flow is low, they will trip the sensor located near the bottom of the gauge which will shut all the lens off. These gauges only read the flow going to the upper and lower column. I would install two more gauges that read the water flow to the ODP and the Electronics. The proper water flow through each leg is 0.7 liters/ minute.
John Ex Philips Service
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Email: gul417-at-mail.usask.ca Name: Guosheng Liu
Organization: U of Saskatchewan
Title-Subject: [Filtered] CM10 TEM: beam shines like a dot but occasionally spreads normal shape.
Message: Hello all,
Our Philips CM10 TEM got a beam issue---it looked like in a dark field mode, concentrated as a tiny point and could not be spread out. But suddenly it might be normal for a few seconds to less than a minute without touching anywhere, then went back to point beam again.
The ODP/IGP vacuum readings are not ideal but seems working before. Anything else could be the cause of this problem? Where/how to check first?
Your help and suggestion are greatly appreciated.
Thank you in advance.
Guosheng
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==============================Original Headers============================== 26, 41 -- From js51-at-princeton.edu Fri Aug 28 08:07:04 2015 26, 41 -- Received: from Princeton.EDU (ppa03.Princeton.EDU [128.112.128.214]) 26, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7SD73cX005193 26, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Aug 2015 08:07:03 -0500 26, 41 -- Received: from csgsmtp202l.Princeton.EDU (csgsmtp202l.Princeton.EDU [140.180.223.155]) 26, 41 -- by ppa03.princeton.edu (8.15.0.59/8.15.0.59) with ESMTPS id t7SD73tJ015940 26, 41 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NOT) 26, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Aug 2015 09:07:03 -0400 26, 41 -- Received: from CSGHUB209W.pu.win.princeton.edu (csghub209w.Princeton.EDU [10.6.60.211]) 26, 41 -- by csgsmtp202l.Princeton.EDU (8.14.4/8.12.9) with ESMTP id t7SD72xr023443; 26, 41 -- Fri, 28 Aug 2015 09:07:03 -0400 26, 41 -- Received: from CSGMBX205W.pu.win.princeton.edu ([169.254.6.214]) by 26, 41 -- CSGHUB209W.pu.win.princeton.edu ([10.6.60.211]) with mapi id 14.03.0235.001; 26, 41 -- Fri, 28 Aug 2015 09:06:14 -0400 26, 41 -- From: "John J. Schreiber" {js51-at-princeton.edu} 26, 41 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 26, 41 -- CC: "gul417-at-mail.usask.ca" {gul417-at-mail.usask.ca} 26, 41 -- Subject: RE: [Microscopy] viaWWW:CM10 TEM: beam shines like a dot but 26, 41 -- occasionally spreads 26, 41 -- Thread-Topic: [Microscopy] viaWWW:CM10 TEM: beam shines like a dot but 26, 41 -- occasionally spreads 26, 41 -- Thread-Index: AQHQ4S0a2QvUrNDWX0GakDze9vrFc54hX44w 26, 41 -- Date: Fri, 28 Aug 2015 13:06:14 +0000 26, 41 -- Message-ID: {3C628C43B600C14EB700249D43D718D31EE1671F-at-CSGMBX205W.pu.win.princeton.edu} 26, 41 -- References: {201508280101.t7S11feP009648-at-ns.microscopy.com} 26, 41 -- In-Reply-To: {201508280101.t7S11feP009648-at-ns.microscopy.com} 26, 41 -- Accept-Language: en-US 26, 41 -- Content-Language: en-US 26, 41 -- X-MS-Has-Attach: 26, 41 -- X-MS-TNEF-Correlator: 26, 41 -- x-originating-ip: [128.112.141.39] 26, 41 -- Content-Type: text/plain; charset="us-ascii" 26, 41 -- MIME-Version: 1.0 26, 41 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2015-08-28_06:,, 26, 41 -- signatures=0 26, 41 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 26, 41 -- suspectscore=0 malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 26, 41 -- classifier=spam adjust=0 reason=mlx scancount=1 engine=8.0.1-1507310000 26, 41 -- definitions=main-1508280211 26, 41 -- Content-Transfer-Encoding: 8bit 26, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7SD73cX005193 ==============================End of - Headers==============================
Also wanted to mention that there are regulators that you can you use to adjust the water flow to the different legs of the water circuit. If you have those max out, then you will need to what is blocking it.
John
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, August 27, 2015 9:02 PM To: John J. Schreiber
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Email: gul417-at-mail.usask.ca Name: Guosheng Liu
Organization: U of Saskatchewan
Title-Subject: [Filtered] CM10 TEM: beam shines like a dot but occasionally spreads normal shape.
Message: Hello all,
Our Philips CM10 TEM got a beam issue---it looked like in a dark field mode, concentrated as a tiny point and could not be spread out. But suddenly it might be normal for a few seconds to less than a minute without touching anywhere, then went back to point beam again.
The ODP/IGP vacuum readings are not ideal but seems working before. Anything else could be the cause of this problem? Where/how to check first?
Your help and suggestion are greatly appreciated.
Thank you in advance.
Guosheng
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==============================Original Headers============================== 26, 41 -- From js51-at-princeton.edu Fri Aug 28 08:15:47 2015 26, 41 -- Received: from Princeton.EDU (ppa04.Princeton.EDU [128.112.128.215]) 26, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7SDFjE8006836 26, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Aug 2015 08:15:46 -0500 26, 41 -- Received: from csgsmtp202l.Princeton.EDU (csgsmtp202l.Princeton.EDU [140.180.223.155]) 26, 41 -- by ppa04.princeton.edu (8.15.0.59/8.15.0.59) with ESMTPS id t7SDFjHj016375 26, 41 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NOT) 26, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Aug 2015 09:15:45 -0400 26, 41 -- Received: from CSGHUB209W.pu.win.princeton.edu (csghub209w.Princeton.EDU [10.6.60.211]) 26, 41 -- by csgsmtp202l.Princeton.EDU (8.14.4/8.12.9) with ESMTP id t7SDFjta031231; 26, 41 -- Fri, 28 Aug 2015 09:15:45 -0400 26, 41 -- Received: from CSGMBX205W.pu.win.princeton.edu ([169.254.6.214]) by 26, 41 -- CSGHUB209W.pu.win.princeton.edu ([10.6.60.211]) with mapi id 14.03.0235.001; 26, 41 -- Fri, 28 Aug 2015 09:14:23 -0400 26, 41 -- From: "John J. Schreiber" {js51-at-princeton.edu} 26, 41 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 26, 41 -- CC: "gul417-at-mail.usask.ca" {gul417-at-mail.usask.ca} 26, 41 -- Subject: RE: [Microscopy] viaWWW:CM10 TEM: beam shines like a dot but 26, 41 -- occasionally spreads 26, 41 -- Thread-Topic: [Microscopy] viaWWW:CM10 TEM: beam shines like a dot but 26, 41 -- occasionally spreads 26, 41 -- Thread-Index: AQHQ4S0a2QvUrNDWX0GakDze9vrFc54hZBDA 26, 41 -- Date: Fri, 28 Aug 2015 13:14:23 +0000 26, 41 -- Message-ID: {3C628C43B600C14EB700249D43D718D31EE16741-at-CSGMBX205W.pu.win.princeton.edu} 26, 41 -- References: {201508280101.t7S11feP009648-at-ns.microscopy.com} 26, 41 -- In-Reply-To: {201508280101.t7S11feP009648-at-ns.microscopy.com} 26, 41 -- Accept-Language: en-US 26, 41 -- Content-Language: en-US 26, 41 -- X-MS-Has-Attach: 26, 41 -- X-MS-TNEF-Correlator: 26, 41 -- x-originating-ip: [128.112.141.39] 26, 41 -- Content-Type: text/plain; charset="us-ascii" 26, 41 -- MIME-Version: 1.0 26, 41 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2015-08-28_06:,, 26, 41 -- signatures=0 26, 41 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 26, 41 -- suspectscore=0 malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 26, 41 -- classifier=spam adjust=0 reason=mlx scancount=1 engine=8.0.1-1507310000 26, 41 -- definitions=main-1508280213 26, 41 -- Content-Transfer-Encoding: 8bit 26, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7SDFjE8006836 ==============================End of - Headers==============================
From ferguson651651615axuht-at-gmail.com Fri Aug 28 10:43:29 2015 Return-Path: {ferguson651651615axuht-at-gmail.com} Received: from gmail.com ([112.216.122.27]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t7SFhPNx013078 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 28 Aug 2015 10:43:27 -0500 Message-ID: {8DBE7A00.FE3B4444-at-gmail.com}
On 28/08/2015 16:05, "js51-at-princeton.edu" {js51-at-princeton.edu} wrote:
} Hello Guosheng, } } If the beam is a pinpoint of light, the most likely cause is water flow. You will see on the Lens Current page that all the main lens values will be close to 0. You should have water flow gauges that are located near the mechanical pump. These have magnetic floats in them and if the flow is low, they will trip the sensor located near the bottom of the gauge which will shut all the lens off. These gauges only read the flow going to the upper and lower column. I would install two more gauges that read the water flow to the ODP and the Electronics. The proper water flow through each leg is 0.7 liters/ minute. } } John } Ex Philips Service }
I don't know if the float meters were a later modification on CM10s, but ours doesn't have them, it just uses a temperature sensor in the heatsinks in the electronics part of the circuit. Our CM100 has all the float meters as you describe.
Ben
-- Research Support Manager and Acting IT Manager MRC Brain Network Dynamics Unit, University of Oxford Department of Pharmacology, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271649 {http://www.mrcbndu.ox.ac.uk/}
==============================Original Headers============================== 7, 32 -- From ben.micklem-at-pharm.ox.ac.uk Fri Aug 28 11:08:57 2015 7, 32 -- Received: from relay11.mail.ox.ac.uk (relay11.mail.ox.ac.uk [129.67.1.162]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7SG8t1H019257 7, 32 -- for {microscopy-at-microscopy.com} ; Fri, 28 Aug 2015 11:08:56 -0500 7, 32 -- Received: from hub04.nexus.ox.ac.uk ([163.1.154.215] helo=HUB04.ad.oak.ox.ac.uk) 7, 32 -- by relay11.mail.ox.ac.uk with esmtp (Exim 4.80) 7, 32 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 7, 32 -- id 1ZVMD1-00088h-ZX 7, 32 -- for microscopy-at-microscopy.com; Fri, 28 Aug 2015 17:08:55 +0100 7, 32 -- Received: from MBX03.ad.oak.ox.ac.uk ([169.254.3.47]) by HUB04.ad.oak.ox.ac.uk 7, 32 -- ([169.254.211.233]) with mapi id 14.03.0169.001; Fri, 28 Aug 2015 17:08:54 7, 32 -- +0100 7, 32 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 7, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 32 -- Subject: Re: [Microscopy] RE: viaWWW:CM10 TEM: beam shines like a dot but 7, 32 -- Thread-Topic: [Microscopy] RE: viaWWW:CM10 TEM: beam shines like a dot but 7, 32 -- Thread-Index: AQHQ4aL8SFC8Qzsr2UWrupw+jlh/954hlGYA 7, 32 -- Date: Fri, 28 Aug 2015 16:08:53 +0000 7, 32 -- Message-ID: {B1D2C2BB-59D5-4520-A08E-7D66BDDEFEC2-at-pharm.ox.ac.uk} 7, 32 -- References: {201508281505.t7SF5TTL002663-at-ns.microscopy.com} 7, 32 -- In-Reply-To: {201508281505.t7SF5TTL002663-at-ns.microscopy.com} 7, 32 -- Accept-Language: en-GB, en-US 7, 32 -- Content-Language: en-US 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- user-agent: Microsoft-MacOutlook/0.0.0.150807 7, 32 -- x-originating-ip: [172.16.150.236] 7, 32 -- Content-Type: text/plain; charset="iso-8859-1" 7, 32 -- Content-ID: {3986874D7A605447A86641B6D44969B8-at-ad.oak.ox.ac.uk} 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7SG8t1H019257 ==============================End of - Headers==============================
Yes, you are correct. The early CM10's did not have a site glass for the water flow. In which the water had only two splits in the circuit. From the water input, half went through the ODP and half goes thru the Lens and electronics. The Power Booster safety circuit shuts off the lens if the heatsink get too hot. I would still recommend adding the flow gauges to adjust the proper water flow.
John
-----Original Message----- X-from: ben.micklem-at-pharm.ox.ac.uk [mailto:ben.micklem-at-pharm.ox.ac.uk] Sent: Friday, August 28, 2015 2:22 PM To: John J. Schreiber
On 28/08/2015 16:05, "js51-at-princeton.edu" {js51-at-princeton.edu} wrote:
} Hello Guosheng, } } If the beam is a pinpoint of light, the most likely cause is water flow. You will see on the Lens Current page that all the main lens values will be close to 0. You should have water flow gauges that are located near the mechanical pump. These have magnetic floats in them and if the flow is low, they will trip the sensor located near the bottom of the gauge which will shut all the lens off. These gauges only read the flow going to the upper and lower column. I would install two more gauges that read the water flow to the ODP and the Electronics. The proper water flow through each leg is 0.7 liters/ minute. } } John } Ex Philips Service }
I don't know if the float meters were a later modification on CM10s, but ours doesn't have them, it just uses a temperature sensor in the heatsinks in the electronics part of the circuit. Our CM100 has all the float meters as you describe.
Ben
-- Research Support Manager and Acting IT Manager MRC Brain Network Dynamics Unit, University of Oxford Department of Pharmacology, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271649 {http://www.mrcbndu.ox.ac.uk/}
==============================Original Headers============================== 7, 32 -- From ben.micklem-at-pharm.ox.ac.uk Fri Aug 28 11:08:57 2015 7, 32 -- Received: from relay11.mail.ox.ac.uk (relay11.mail.ox.ac.uk [129.67.1.162]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7SG8t1H019257 7, 32 -- for {microscopy-at-microscopy.com} ; Fri, 28 Aug 2015 11:08:56 -0500 7, 32 -- Received: from hub04.nexus.ox.ac.uk ([163.1.154.215] helo=HUB04.ad.oak.ox.ac.uk) 7, 32 -- by relay11.mail.ox.ac.uk with esmtp (Exim 4.80) 7, 32 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 7, 32 -- id 1ZVMD1-00088h-ZX 7, 32 -- for microscopy-at-microscopy.com; Fri, 28 Aug 2015 17:08:55 +0100 7, 32 -- Received: from MBX03.ad.oak.ox.ac.uk ([169.254.3.47]) by HUB04.ad.oak.ox.ac.uk 7, 32 -- ([169.254.211.233]) with mapi id 14.03.0169.001; Fri, 28 Aug 2015 17:08:54 7, 32 -- +0100 7, 32 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 7, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 32 -- Subject: Re: [Microscopy] RE: viaWWW:CM10 TEM: beam shines like a dot but 7, 32 -- Thread-Topic: [Microscopy] RE: viaWWW:CM10 TEM: beam shines like a dot but 7, 32 -- Thread-Index: AQHQ4aL8SFC8Qzsr2UWrupw+jlh/954hlGYA 7, 32 -- Date: Fri, 28 Aug 2015 16:08:53 +0000 7, 32 -- Message-ID: {B1D2C2BB-59D5-4520-A08E-7D66BDDEFEC2-at-pharm.ox.ac.uk} 7, 32 -- References: {201508281505.t7SF5TTL002663-at-ns.microscopy.com} 7, 32 -- In-Reply-To: {201508281505.t7SF5TTL002663-at-ns.microscopy.com} 7, 32 -- Accept-Language: en-GB, en-US 7, 32 -- Content-Language: en-US 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- user-agent: Microsoft-MacOutlook/0.0.0.150807 7, 32 -- x-originating-ip: [172.16.150.236] 7, 32 -- Content-Type: text/plain; charset="iso-8859-1" 7, 32 -- Content-ID: {3986874D7A605447A86641B6D44969B8-at-ad.oak.ox.ac.uk} 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7SG8t1H019257 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 40 -- From js51-at-princeton.edu Fri Aug 28 14:37:18 2015 16, 40 -- Received: from Princeton.EDU (ppa02.Princeton.EDU [128.112.128.216]) 16, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7SJbGaQ021406 16, 40 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Aug 2015 14:37:16 -0500 16, 40 -- Received: from csgsmtp202l.Princeton.EDU (csgsmtp202l.Princeton.EDU [140.180.223.155]) 16, 40 -- by ppa02.princeton.edu (8.15.0.59/8.15.0.59) with ESMTPS id t7SJbFmF023462 16, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NOT) 16, 40 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Aug 2015 15:37:15 -0400 16, 40 -- Received: from CSGHUB208W.pu.win.princeton.edu (csghub208w.Princeton.EDU [10.6.60.210]) 16, 40 -- by csgsmtp202l.Princeton.EDU (8.14.4/8.12.9) with ESMTP id t7SJbFFY019099; 16, 40 -- Fri, 28 Aug 2015 15:37:15 -0400 16, 40 -- Received: from CSGMBX205W.pu.win.princeton.edu ([169.254.6.214]) by 16, 40 -- CSGHUB208W.pu.win.princeton.edu ([10.6.60.210]) with mapi id 14.03.0235.001; 16, 40 -- Fri, 28 Aug 2015 15:36:14 -0400 16, 40 -- From: "John J. Schreiber" {js51-at-princeton.edu} 16, 40 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 16, 40 -- CC: "ben.micklem-at-pharm.ox.ac.uk" {ben.micklem-at-pharm.ox.ac.uk} , 16, 40 -- "gul417-at-mail.usask.ca" {gul417-at-mail.usask.ca} 16, 40 -- Subject: RE: [Microscopy] viaWWW:CM10 TEM: beam shines like a dot but 16, 40 -- Thread-Topic: [Microscopy] viaWWW:CM10 TEM: beam shines like a dot but 16, 40 -- Thread-Index: AQHQ4b5nXI9q1RJ78kaKdByefdTHbp4hxFhw 16, 40 -- Date: Fri, 28 Aug 2015 19:36:13 +0000 16, 40 -- Message-ID: {3C628C43B600C14EB700249D43D718D31EE16C90-at-CSGMBX205W.pu.win.princeton.edu} 16, 40 -- References: {201508281821.t7SILlFU017730-at-ns.microscopy.com} 16, 40 -- In-Reply-To: {201508281821.t7SILlFU017730-at-ns.microscopy.com} 16, 40 -- Accept-Language: en-US 16, 40 -- Content-Language: en-US 16, 40 -- X-MS-Has-Attach: 16, 40 -- X-MS-TNEF-Correlator: 16, 40 -- x-originating-ip: [128.112.141.39] 16, 40 -- Content-Type: text/plain; charset="us-ascii" 16, 40 -- MIME-Version: 1.0 16, 40 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2015-08-28_09:,, 16, 40 -- signatures=0 16, 40 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 16, 40 -- suspectscore=0 malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 16, 40 -- classifier=spam adjust=0 reason=mlx scancount=1 engine=8.0.1-1507310000 16, 40 -- definitions=main-1508280310 16, 40 -- Content-Transfer-Encoding: 8bit 16, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7SJbGaQ021406 ==============================End of - Headers==============================
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] TEM processing of Histology Section.
Message: Dear Listener,
I have one user, who wants to carry out TEM analysis of his Histology samples. He has only histology slide stained with H&E.
Has anyone done this kind of work before? Kindly give the best suggestion from your experience.
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Email: gul417-at-mail.usask.ca Name: Guosheng Liu
Organization: University of Saskathewan
Title-Subject: [Filtered] CM10 TEM pinpoint beam problem --Thank all of you for the help
Message: Dear Lister,
First of all I want to thank you for the prompt replies regarding the pinpoint beam issue on our CM10.
In summary ,majority of the comments considered that the bad water flow led to the LENS current On/OFF intermittently due to the lens power heatsink's overheating. This is most likely what is happening in our CM10. Our CM10 has been with cooling issue for years. We have replaced a new circulating water chiller for the system, and also monitor the temperature for the column with temperature-sensor-type stickers. We have used CLR to clean the cooling system regularly (last time was half year ago). Even though, the temperature of bottom part of the gun column is still high, around 82-96F(28-35C) during operation (e.g. 80KV). The temp setting for the chiller is at 14C now. Definitely there is still some clog in the cooling system. The top part of the column tempertaure is used to be OK (always around 20C) but today it is 28C . Anyhow I'll first do the CLR flush (at least overnight) to try to boost water flow.
When I check the lens current, it indicated that Projector (1&2)Lens were down. Here are the readings (twice) when beam was normal and pinpoint: ______________________________ C1 424/294 424/424 mA Obj 110/115 120/117 Diff 787/549 787/795 Interm 3/3 3/124 Proj1 2190/1522 5/4 Proj2 1789/1248 -1/-1 _____________________________
My question is: is there any way to check the cooling hose from outside of the column which links to the projector lens part?
Except for the bad cooling water for lens, it also could be the problem related to the failure of C2 condenser board, projector lens' fuse, or dirty beam path or even HT board as others pointed out in the comments. I'll check these later if not working.
Thanks again.
Guosheng
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If you mean preparing an H&E section for TEM, I suspect you are in for a disappointing result. I haven't done it for an H&E section but have done it for an unstained paraffin section. I osmicated the section, infiltrated with resin and polymerized resin on the surface of the slide. I then popped it off the slide using liquid nitrogen and mounted en face on an epoxy block so I could section it. The tissue was recognizable in the TEM but he quality of the tissue preservation was terrible. It looked vacuolated. I am always amazed how "acceptable" tissue fixation looks like at the LM level but how bad it looks like once you go to TEM. TEM is tedious and demanding enough with optimally fixed tissues so it is best to handicapping your chances before you even start. Unless the tissue is something incredibly rare, it is worth repeating with proper fixation and embedding for TEM.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] TEM processing of Histology Section.
Message: Dear Listener,
I have one user, who wants to carry out TEM analysis of his Histology samples. He has only histology slide stained with H&E.
Has anyone done this kind of work before? Kindly give the best suggestion from your experience.
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I will second that Nizetian wisdom. As I just posted in response to someone wondering if a paraffin section could be prepared for electron microscopy, TEM is tedious and demanding enough with optimally fixed tissues so it is best to avoid handicapping your chances before you even start. Unless the tissue is something incredibly rare, it is worth repeating with proper fixation and embedding for TEM.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
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Hi Joseph!
Summary (you don't need the details to take a decision): "not optimal" is mildly put. Leaving biological material unfixed at -18°C (it is probably the worst method to preserver biological material) and expecting to see anything meaningful in TEM is not realistic. If you want to use TEM, you are interested in fine morphology right? Well you won't get fine morphological information this way.
Details (if you want to know why): 1) Upon freezing and especially slowly freezing like it happens when you put a specimen at -18°C, the water in the tissue and cells crystallizes. Nice spiky crystals. The crystals don't care about the biological structures (like membranes), they grow through them, perforating them. Upon thawing, the membrane are literally cut in pieces, leaving you with a biological soup with very few original morphological properties. 2) The cellular enzymes have had plenty of time to do whatever job they have to do 3) Big osmotic issues. Slowly freezing produces extreme osmotic forces because while some water freezes the remaining water which is in liquid state sees its salt concentration dramatically increase
Hope you can convince your colleagues to invest time and money in something more useful (like reading a book about the usefulness of fixatives).
Stephane
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Subject: [Microscopy] viaWWW:Frozen Insects for TEM To: nizets2-at-yahoo.com Date: Friday, August 21, 2015, 1:45 AM
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Email: joseph.mowery-at-ars.usda.gov Name: Joseph Mowery
Organization: USDA ARS SGIL ECMU
Title-Subject: [Filtered] Frozen Insects for TEM
Message: Greetings,
We're interested in processing moths for TEM, but the moths have been stored in a 0F (-18C) freezer for a few weeks or months. Will it still be possible to immerses these moths in glutaraldehyde and process them for conventional TEM? I understand the ultrastructure may not be optimal, but has anyone had success processing frozen insects for TEM?
Thanks, -Joe
Joe Mowery | Biologist Electron and Confocal Microscopy Unit USDA Agricultural Research Service Lab 301-504-9027 | Mobile 817-821-8566 joseph.mowery-at-ars.usda.gov
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==============================Original Headers============================== 18, 32 -- From PhillipsT-at-missouri.edu Sat Aug 29 15:04:59 2015 18, 32 -- Received: from um-tip1-missouri-out.um.umsystem.edu (um-tip1-missouri-out.um.umsystem.edu [198.209.49.135]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7TK4w2Q008038 18, 32 -- for {microscopy-at-microscopy.com} ; Sat, 29 Aug 2015 15:04:59 -0500 18, 32 -- X-IronPort-Anti-Spam-Filtered: true 18, 32 -- X-IronPort-Anti-Spam-Result: A2AhBQCYD+JV/9KeoM9bA4MbVGkGrTOSU4V7AoEkPBABAQEBAQEBgQqEIwEBAQROOQICAQgRAwECCwIKARcWCxABEwEJCAIEAQkJCBUEh3gDEg22dQMKRgGEXgEBAQEBAQEDAQEBAQEBAQEBAQEXBIZshHuCTw0RCQGBMRACAR8PEhIFBgwBAYMEgRQFhXQMgSuFRDmFAIMZAYUGhS5SBoMxRoNsjTKDUINsJoINAhyBVHEBAQEBgQMHFyOBBQEBAQ 18, 32 -- X-IPAS-Result: A2AhBQCYD+JV/9KeoM9bA4MbVGkGrTOSU4V7AoEkPBABAQEBAQEBgQqEIwEBAQROOQICAQgRAwECCwIKARcWCxABEwEJCAIEAQkJCBUEh3gDEg22dQMKRgGEXgEBAQEBAQEDAQEBAQEBAQEBAQEXBIZshHuCTw0RCQGBMRACAR8PEhIFBgwBAYMEgRQFhXQMgSuFRDmFAIMZAYUGhS5SBoMxRoNsjTKDUINsJoINAhyBVHEBAQEBgQMHFyOBBQEBAQ 18, 32 -- Received: from um-ncas5.um.umsystem.edu ([207.160.158.210]) 18, 32 -- by um-tip1-exch-relay.um.umsystem.edu with ESMTP; 29 Aug 2015 15:04:57 -0500 18, 32 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.122]) by 18, 32 -- UM-NCAS5.um.umsystem.edu ([207.160.158.210]) with mapi id 14.03.0248.002; 18, 32 -- Sat, 29 Aug 2015 15:04:57 -0500 18, 32 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 18, 32 -- To: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} , 18, 32 -- "'microscopy-at-microscopy.com'" 18, 32 -- {microscopy-at-microscopy.com} 18, 32 -- Subject: RE: [Microscopy] Re: viaWWW:Frozen Insects for TEM 18, 32 -- Thread-Topic: [Microscopy] Re: viaWWW:Frozen Insects for TEM 18, 32 -- Thread-Index: AQHQ4JOxUE0NH18Vakmapw5as7Pf+p4jagLg 18, 32 -- Date: Sat, 29 Aug 2015 20:04:56 +0000 18, 32 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DE723525E-at-UM-MBX-T02.um.umsystem.edu} 18, 32 -- References: {201508270643.t7R6hV4f025083-at-ns.microscopy.com} 18, 32 -- In-Reply-To: {201508270643.t7R6hV4f025083-at-ns.microscopy.com} 18, 32 -- Accept-Language: en-US 18, 32 -- Content-Language: en-US 18, 32 -- X-MS-Has-Attach: 18, 32 -- X-MS-TNEF-Correlator: 18, 32 -- x-originating-ip: [207.160.158.193] 18, 32 -- Content-Type: text/plain; charset="iso-8859-1" 18, 32 -- MIME-Version: 1.0 18, 32 -- Content-Transfer-Encoding: 8bit 18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7TK4w2Q008038 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dscibro-at-gmail.com Sun Aug 30 19:27:30 2015 Return-Path: {mike.sfsd4f564s6df45dscibro-at-gmail.com} Received: from gmail.com (sd27-255-84-105.dss-host.net [27.255.84.105] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t7V0RR8I029922 for {microscopylistserverarchive5-at-microscopy.com} ; Sun, 30 Aug 2015 19:27:29 -0500 Message-ID: {9B26AB46.F336A223-at-gmail.com}
X-from: kaltbeit-at-mpip-mainz.mpg.de
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Email: kaltbeit-at-mpip-mainz.mpg.de Name: Anke Kaltbeitzel
Organization: MPI Polymer-research
Title-Subject: [Filtered] Elastic montage/stitching of point-maps
Message: I'm trying to stitch overlapping SEM images. The image series also includes an overview screen with less resolution.
Due to electrostatic charging there is elastic deformation of the SEM image. Sputtering or averaging during image acquisition won't help because they reduce the image quality. However, using beads for registration should make it a task that can be solved. Of course in some images some beads are missing after object recognition (especially for the overview) but most points are detected.
The naked eye has little trouble in matching the beads in the images. So I thought it was an easy task for image analysis software...
I tried TRACKEM2 for elastic montage, but it failed (for me) to register the beads, maybe the elastic algorithm works better for larger scale features. Defining landmarks seems to work only for affine transformations in TRACKEM2(and this one is definitly elastic) The coherent point drift algorithm should work for elastic bead registration. But the overlap of the images is never } 50%.
Is there some software that easily solves the problem?
Anke Kaltbeitzel
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==============================Original Headers============================== 20, 40 -- From microscopy.listserver-at-gmail.com Mon Aug 31 07:24:26 2015 20, 40 -- Received: from mail-qg0-f52.google.com (mail-qg0-f52.google.com [209.85.192.52]) 20, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7VCOQKC021467 20, 40 -- for {microscopy-at-microscopy.com} ; Mon, 31 Aug 2015 07:24:26 -0500 20, 40 -- Received: by qgeb6 with SMTP id b6so64081571qge.3 20, 40 -- for {microscopy-at-microscopy.com} ; Mon, 31 Aug 2015 05:24:26 -0700 (PDT) 20, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 20, 40 -- d=gmail.com; s=20120113; 20, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 20, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 20, 40 -- bh=blPnVl2w5EpjU4ZNkcI+NsRUlzhgf9pIKalTJWy3zfY=; 20, 40 -- b=uE2xtciFqpnbE8qA5S2E1x9jMzt59MuKUb6x2ycVspD2GSKwyw88XhZids5FRPBSXR 20, 40 -- TCde65MGp3k98sxjNwlxGiUkZcuaHDCVD2rpNOvYe+muDapuwbm5GpNsAhoEWRIp+FnD 20, 40 -- hzdK7y2/S1L8JDKXcDjLI9raRcK1ATdtT1loaJ1aWXW0/20lRJejPm8P6wB7kAHls5OX 20, 40 -- CO5t3zn4eJBz0kUYcHk19Ypuj6lJFpPZQ72Dnne3uzm0AD0J71BAkn/r7ms6B99Cjqat 20, 40 -- WmtPyt5b667izzsTAnboqs9onqqAIvgUQvCCcZjgygw0v2mw5wU0wp/WUJ6WJiweylhQ 20, 40 -- wlQw== 20, 40 -- X-Received: by 10.140.236.194 with SMTP id h185mr38919786qhc.45.1441023865823; 20, 40 -- Mon, 31 Aug 2015 05:24:25 -0700 (PDT) 20, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 20, 40 -- by smtp.googlemail.com with ESMTPSA id o64sm3240148qge.44.2015.08.31.05.24.25 20, 40 -- for {microscopy-at-microscopy.com} 20, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 20, 40 -- Mon, 31 Aug 2015 05:24:25 -0700 (PDT) 20, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 20, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 20, 40 -- {microscopylistserver-noreply-at-microscopy.com} 20, 40 -- Subject: viaWWW:Elastic montage/stitching of point-maps 20, 40 -- References: {201508310929.t7V9Tfnh018100-at-ns.microscopy.com} 20, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 40 -- X-Forwarded-Message-Id: {201508310929.t7V9Tfnh018100-at-ns.microscopy.com} 20, 40 -- Message-ID: {55E44777.9050800-at-microscopy.com} 20, 40 -- Date: Mon, 31 Aug 2015 07:24:23 -0500 20, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 20, 40 -- Gecko/20100101 Thunderbird/38.2.0 20, 40 -- MIME-Version: 1.0 20, 40 -- In-Reply-To: {201508310929.t7V9Tfnh018100-at-ns.microscopy.com} 20, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
It is not (IT HAS NOT BEEN!) often that {someone} (via the MSA Listserver) requests such measures/procedures.. this probably due to the decrease of med.-diagnostic EM-ists (unfortunately many medico-diagnostic EM-Labs have been and further will be extincted) out in the wild. He/she must be a young pathologist or clinician, I guess.
Seconding 100% Prof. Phillips' opinion (and making no difference between an H&E stained = deparaffinized or unstained paraffin section): { Garbage in {-} Garbage out } : If the tissue initially fixed poorly then poor / bad preservation of ultrastructural detail will be the "natural" consequence.
BUT: SOMETIMES careful and sophisticated reprocessing of - selected areas of - either tissue from whole paraffin blocks or also a H&E-section only can yield some interesting and unforeseen result in terms of diagnosis (viral, bacteria, etc. etc., see also below).
So in the end: It always will depend on the circumstance and TASK of the study whether such a re-embedding/reprocessing (which might be sumptuous and/or a bit complex to accomplish) will yield something of value (e. g. especially something diagnostic ..) - worth to be documented.
In my 35 years EM-career I have done about 100-150 re-embeddings (from paraffin blocks as well as from deparaffinized H&E and or pre-embedding-IHC-sections) and the scientifically useable yield was -estimated - about 65-70% [esp. for evaluating the preembedding Immunolocaliztion of markers in (DAB-treated)IHC sections ].
There have been some articles in MICROSCOPY TODAY*), and other journals**) on that matter, and I know about there also are some questions and replies available within the MSA-Listserver Archives***).
Principal keywords for search in the MSA archives or also a {google search} : "reprocessing", "reembedding", "re-embedding",
Some examples for your convenience: : *)e.g.: ESTRADA JC et al, TEM of Paraffin-Embedded H&E Stained Sections for viral Diagnosis (an Unusual Papovavirus Case) Microscopy Today, Sept. 2005, pp. 22 - and - 24 (23 = full page advertisement)
**) see e.g. also [naturally not exhaustive!]: J. BURNS, [Technical Methods] Preparation of thin epoxy resin sections from thick sections of paraffin-embedded material in: J Clin Path 23(7),1970 p.643-645
or: Van den Bergh Weerman M.A., DINGEMANS K.P.: [New Techniques] Rapid deparaffinization for electron microscopy, Ultrastructural Pathology, 7:55-57,1984
or: S. Widéhn, and L.-G. Kindblom: [New Techniques] A Rapid and Simple Method for Electron Microscopy of Paraffin-Embedded Tissue Ultrastructural Pathology, 12: 131 - 136, 1988
or LIGHEZAN R, et al. {The value of the reprocessing method of paraffin-embedded biopsies for transmission electron microscopy} in: Romanian Journal of Morphology and Embryology 2009, 50(4):613-617 Abstract Transmission electron microscopy (TEM) implies an elaborate preparation protocol that includes: fixation in glutaraldehyde followed by osmium tetraoxide postfixation, specimen dehydration, infiltration, resin embedding, ultrathin sectioning and staining with heavy metal salts. The aim of TEM is to examine the ultrastructure of specimens in ways that cannot be examined using other equipments or techniques. In some cases, when the requirement for TEM were made after tissue collection, useful information can be obtained from reprocessing the formalin-fixed, wax-embedded tissue used for light microscopy. Keywords: reprocessing method, transmission electron microscopy.
***) e.g. {Reprocessing paraffin material to EM} : thread: Sat, 22 Jan 2005 11:50:44 -0600 until Tue, 25 Jan 2005 07:24:15 -0600
If you need articles (pdf) (as mentioned & more...) you certainly could ask me to send them off list.
Best wishes, good luck, and regards, Wolfgang MUSS PhD SALZBURG, Austria Member of MSA
} -----Ursprüngliche Nachricht----- } Von: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] } Gesendet: Samstag, 29. August 2015 22:43 } An: Muß Wolfgang } Betreff: [Microscopy] Re: TEM processing of Histology Section } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe www.microscopy.com -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } If you mean preparing an H&E section for TEM, I suspect you are in for } a disappointing result. } I haven't done it for an H&E section but have done it for an unstained } paraffin section. } I osmicated the section, infiltrated with resin and polymerized resin } on the surface of the slide. } I then popped it off the slide using liquid nitrogen and mounted en } face on an epoxy block so I could section it. } The tissue was recognizable in the TEM but he quality of the tissue } preservation was terrible. } It looked vacuolated. I am always amazed how "acceptable" tissue } fixation looks like at the LM level but how bad it looks like once you } go to TEM. } TEM is tedious and demanding enough with optimally fixed tissues so it } is best to handicapping your chances before you even start. } Unless the tissue is something incredibly rare, it is worth repeating } with proper fixation and embedding for TEM. } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: microscopy.listserver-at-gmail.com } [mailto:microscopy.listserver-at-gmail.com] } Sent: Friday, August 28, 2015 6:42 PM } To: Phillips, Thomas E. } Subject: [Microscopy] TEM processing of Histology Section } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } X-from: ravi.thakkar369-at-gmail.com } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://microscopy.com/MLFormMail.html } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both ravi.thakkar369-at-gmail.com as well as the } Microscopy Listserver } ----------------------------------------------------------------------- } ---- } Email: ravi.thakkar369-at-gmail.com } Name: Ravi } Title-Subject: [Filtered] TEM processing of Histology Section. } Message: Dear Listener, } I have one user, who wants to carry out TEM analysis of his Histology } samples. He has only histology slide stained with H&E. } Has anyone done this kind of work before? Kindly give the best } suggestion from your experience. } Login Host: 129.130.145.144 } Listserver Email Form V - 20120416 } ----------------------------------------------------------------------- } ---- } - ---===[|]===--- } - } =========================================== } Do not reply to this message it is from the Microscopy Listserver NO- } REPLY forwarding system. 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==============================Original Headers============================== 25, 38 -- From W.Muss-at-salk.at Mon Aug 31 09:42:09 2015 25, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 25, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t7VEg9g0012171 25, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 31 Aug 2015 09:42:09 -0500 25, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 25, 38 -- by hermes.salk.at (Postfix) with ESMTP id 7BF7A6331CD 25, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 31 Aug 2015 16:42:07 +0200 (CEST) 25, 38 -- X-Scanned-By: SALK-Content-Filter 25, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 25, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 25, 38 -- with ESMTP id isXEh9yLP8-9 for {Microscopy-at-microscopy.com} ; 25, 38 -- Mon, 31 Aug 2015 16:42:07 +0200 (CEST) 25, 38 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 25, 38 -- by hermes.salk.at (Postfix) with ESMTP id 472856331E4 25, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 31 Aug 2015 16:42:07 +0200 (CEST) 25, 38 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 25, 38 -- by N1EX198.lks.local with ESMTP; 31 Aug 2015 16:52:39 +0200 25, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 25, 38 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 25, 38 -- 14.03.0248.002; Mon, 31 Aug 2015 16:42:07 +0200 25, 38 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 25, 38 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 25, 38 -- Subject: [Microscopy] Re: TEM processing of Histology Section 25, 38 -- Thread-Topic: [Microscopy] Re: TEM processing of Histology Section 25, 38 -- Thread-Index: AQHQ4/TNWux9oMo51U+oguWBuuHX0Q== 25, 38 -- Date: Mon, 31 Aug 2015 14:42:06 +0000 25, 38 -- Message-ID: {3D22E6EE65F39E448DCE416281537671470E235D-at-N2DT131.lksdom21.lks.local} 25, 38 -- References: {201508292043.t7TKhC8l016939-at-ns.microscopy.com} 25, 38 -- In-Reply-To: {201508292043.t7TKhC8l016939-at-ns.microscopy.com} 25, 38 -- Accept-Language: de-DE, en-US 25, 38 -- Content-Language: de-DE 25, 38 -- X-MS-Has-Attach: 25, 38 -- X-MS-TNEF-Correlator: 25, 38 -- x-originating-ip: [192.168.42.2] 25, 38 -- Content-Type: text/plain; charset="iso-8859-1" 25, 38 -- MIME-Version: 1.0 25, 38 -- Content-Transfer-Encoding: 8bit 25, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t7VEg9g0012171 ==============================End of - Headers==============================
I know you just asked if we've already done this kind of work, not what we think about it. However I must second both Thomas and Wolfgang. Technically it is not such a big challenge but we like to know if what we do makes sense somehow. You rarely give yourself the hassle of TEM preparation because it is cool (although it IS cool), usually one has a precise purpose in mind. If your colleague/client wants fine morphological information, he will be very disappointed. Regards, Stephane
-------------------------------------------- On Mon, 8/31/15, W.Muss-at-salk.at {W.Muss-at-salk.at} wrote:
Subject: [Microscopy] Re: TEM processing of Histology Section To: nizets2-at-yahoo.com Date: Monday, August 31, 2015, 4:47 PM
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APOLOGIZE for Lengthiness-
Dear Ravi,
It is not (IT HAS NOT BEEN!) often that {someone} (via the MSA Listserver) requests such measures/procedures.. this probably due to the decrease of med.-diagnostic EM-ists (unfortunately many medico-diagnostic EM-Labs have been and further will be extincted) out in the wild. He/she must be a young pathologist or clinician, I guess.
Seconding 100% Prof. Phillips' opinion (and making no difference between an H&E stained = deparaffinized or unstained paraffin section): { Garbage in {-} Garbage out } : If the tissue initially fixed poorly then poor / bad preservation of ultrastructural detail will be the "natural" consequence.
BUT: SOMETIMES careful and sophisticated reprocessing of - selected areas of - either tissue from whole paraffin blocks or also a H&E-section only can yield some interesting and unforeseen result in terms of diagnosis (viral, bacteria, etc. etc., see also below).
So in the end: It always will depend on the circumstance and TASK of the study whether such a re-embedding/reprocessing (which might be sumptuous and/or a bit complex to accomplish) will yield something of value (e. g. especially something diagnostic ..) - worth to be documented.
In my 35 years EM-career I have done about 100-150 re-embeddings (from paraffin blocks as well as from deparaffinized H&E and or pre-embedding-IHC-sections) and the scientifically useable yield was -estimated - about 65-70% [esp. for evaluating the preembedding Immunolocaliztion of markers in (DAB-treated)IHC sections ].
There have been some articles in MICROSCOPY TODAY*), and other journals**) on that matter, and I know about there also are some questions and replies available within the MSA-Listserver Archives***).
Principal keywords for search in the MSA archives or also a {google search} : "reprocessing", "reembedding", "re-embedding",
Some examples for your convenience: : *)e.g.: ESTRADA JC et al, TEM of Paraffin-Embedded H&E Stained Sections for viral Diagnosis (an Unusual Papovavirus Case) Microscopy Today, Sept. 2005, pp. 22 - and - 24 (23 = full page advertisement)
**) see e.g. also [naturally not exhaustive!]: J. BURNS, [Technical Methods] Preparation of thin epoxy resin sections from thick sections of paraffin-embedded material in: J Clin Path 23(7),1970 p.643-645
or: Van den Bergh Weerman M.A., DINGEMANS K.P.: [New Techniques] Rapid deparaffinization for electron microscopy, Ultrastructural Pathology, 7:55-57,1984
or: S. Widéhn, and L.-G. Kindblom: [New Techniques] A Rapid and Simple Method for Electron Microscopy of Paraffin-Embedded Tissue Ultrastructural Pathology, 12: 131 - 136, 1988
or LIGHEZAN R, et al. {The value of the reprocessing method of paraffin-embedded biopsies for transmission electron microscopy} in: Romanian Journal of Morphology and Embryology 2009, 50(4):613-617 Abstract Transmission electron microscopy (TEM) implies an elaborate preparation protocol that includes: fixation in glutaraldehyde followed by osmium tetraoxide postfixation, specimen dehydration, infiltration, resin embedding, ultrathin sectioning and staining with heavy metal salts. The aim of TEM is to examine the ultrastructure of specimens in ways that cannot be examined using other equipments or techniques. In some cases, when the requirement for TEM were made after tissue collection, useful information can be obtained from reprocessing the formalin-fixed, wax-embedded tissue used for light microscopy. Keywords: reprocessing method, transmission electron microscopy.
***) e.g. {Reprocessing paraffin material to EM} : thread: Sat, 22 Jan 2005 11:50:44 -0600 until Tue, 25 Jan 2005 07:24:15 -0600
If you need articles (pdf) (as mentioned & more...) you certainly could ask me to send them off list.
Best wishes, good luck, and regards, Wolfgang MUSS PhD SALZBURG, Austria Member of MSA
} -----Ursprüngliche Nachricht----- } Von: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] } Gesendet: Samstag, 29. August 2015 22:43 } An: Muß Wolfgang } Betreff: [Microscopy] Re: TEM processing of Histology Section } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe www.microscopy.com -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } If you mean preparing an H&E section for TEM, I suspect you are in for } a disappointing result. } I haven't done it for an H&E section but have done it for an unstained } paraffin section. } I osmicated the section, infiltrated with resin and polymerized resin } on the surface of the slide. } I then popped it off the slide using liquid nitrogen and mounted en } face on an epoxy block so I could section it. } The tissue was recognizable in the TEM but he quality of the tissue } preservation was terrible. } It looked vacuolated. I am always amazed how "acceptable" tissue } fixation looks like at the LM level but how bad it looks like once you } go to TEM. } TEM is tedious and demanding enough with optimally fixed tissues so it } is best to handicapping your chances before you even start. } Unless the tissue is something incredibly rare, it is worth repeating } with proper fixation and embedding for TEM. } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: microscopy.listserver-at-gmail.com } [mailto:microscopy.listserver-at-gmail.com] } Sent: Friday, August 28, 2015 6:42 PM } To: Phillips, Thomas E. } Subject: [Microscopy] TEM processing of Histology Section } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } X-from: ravi.thakkar369-at-gmail.com } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://microscopy.com/MLFormMail.html } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both ravi.thakkar369-at-gmail.com as well as the } Microscopy Listserver } ----------------------------------------------------------------------- } ---- } Email: ravi.thakkar369-at-gmail.com } Name: Ravi } Title-Subject: [Filtered] TEM processing of Histology Section. } Message: Dear Listener, } I have one user, who wants to carry out TEM analysis of his Histology } samples. He has only histology slide stained with H&E. } Has anyone done this kind of work before? Kindly give the best } suggestion from your experience. } Login Host: 129.130.145.144 } Listserver Email Form V - 20120416 } ----------------------------------------------------------------------- } ---- } - ---===[|]===--- } - } =========================================== } Do not reply to this message it is from the Microscopy Listserver NO- } REPLY forwarding system. 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From hanja07656515151kuzqo-at-gmail.com Tue Sep 1 17:54:36 2015 Return-Path: {hanja07656515151kuzqo-at-gmail.com} Received: from gmail.com ([222.121.136.95]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t81MsXC7025587 for {microscopylistserverarchive5-at-microscopy.com} ; Tue, 1 Sep 2015 17:54:35 -0500 Message-ID: {EC44B940.EA4C0709-at-gmail.com}
X-from: stacie-at-ems-secure.com
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Title-Subject: [Filtered] ImmunoGold Fall Workshop
Message: Aurion and Electron Microscopy Sciences are pleased to announce the Fall Workshop, Aurion ImmunoGold Silver Staining, to be held at the University of Maryland's Electron Microscopy Core Imaging Facility in Baltimore, October 28-30, 2015. For more information, visit http://www.emsdiasum.com/microscopy/products/immunogold/workshop.aspx or contact me directly. Thank you, Stacie Kirsch President, Electron Microscopy Sciences 1560 Industry Road Hatfield, PA 19440 Tel: 215-412-8400 Fax: 215-412-8450 E-Mail: sgkcck-at-aol.com or stacie-at-ems-secure.com Web: www.emsdiasum.com
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Email: lfox1-at-luc.edu Name: Linda M Fox
Organization: Loyola University SSOM - Core Imaging Facility
Title-Subject: [Filtered] Free JEOL 840A - SEM
Message: Free to a Good Home JEOL scanning electron microscope - JSM-840A -all manuals, service records and expendable supplies Purchased 1986 SEM working well, not in use for the past year, but under service contract until Aug 2012. Has digital frame grabber/software/computer also Polaroid camera Computer for digital imaging no longer boots up properly. Ancillary equipment: Polaron critical point dryer (and a spare for parts) and Polaron sputter coater, misc stubs, silver paint, carbon blanks etc. **Possibility of a used Haskaris water chiller** Take everything  you move it.
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] EELS & EFTEM School October 2015 In California
Message: October13-16, 2015 Gatan R&D Headquarters, Pleasanton, CA
This course reviews the basic theory and practice of EELS imaging and analysis in the TEM, but its main emphasis is on practical techniques, optimum deployment of analytical TEM hardware and software systems, and advanced EELS and EFTEM applications. Some prior experience with EELS, EFTEM, and analytical TEM systems is recommended, as is a good familiarity with TEM/STEM instrumentation and techniques. By the end of the course, participants can expect to know how best to optimize the performance of their EELS hardware as well as their EELS and EFTEM experimental setups in order to capture and extract the maximum amount of information from their TEM samples.
If you are interested, please register online at: http://www.gatan.com/company/events/eels-eftem-analysis-training-school-october-2015
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The fall classes have started, schedules are filling up, we are hoping you will have room for the November 4th Fall MRL conference featuring Bioengineering!
We have some world class speakers in bioengineering featured.
You can find out more about the speakers, the student and post doc competition, and the vendor fair here: http://mrl.illinois.edu/mrl-biological-conference-2015
COMPETITORS:
Student and post doc competitors will earn a monetary fee, you need only to have a biological topic that includes equipment we use ( i.e. AFM, TEM, FIB, SEM, Light Microscopy, Confocal etc) Both a 15 min speech and a poster is expected from each participant. Enter the competition at the online registration, there will be a box for you to check, be prepared to upload a 1/2 page abstract, and if you are chosen for the competition, the registration fee will be refunded to you.
Registration is up, and is only $20. For out of town guests, we have a hotel only 2 small blocks away.
Email me with questions.
Lou Ann
VENDORS!!
We have a vendor fair for the participants.
If you have not registered already:
Vendors will have a chance for a one day table display, with perhaps opportunity for demonstrations ( email me), for $200. The next day, which is open for tours and my open lab, but if you have demostraions, you are also welcome to stay for the next day, we can set you up for demoâs the 2nd day.
Registration includes one vendor and one table, additional folks to your table only have to register $20 as a participant. ( covering food etc with this)
I hope to see you there, having great vendors is what makes this conference ever so much more special!
Thanks,
Lou Ann
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Hours: 7am-3:30 pm
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Email: lfox1-at-luc.edu Name: Linda M Fox
Organization: Loyola University SSOM - Core Imaging Facility
Title-Subject: [Filtered] Free SEM...now spoken for
Message: Thank all of you who expressed interest in our JEOl 840A SEM. We now have several interested parties. If any or all fall through, I will re-post for others to throw a hat into the ring.
Thank you all,
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we noticed changes of temperature in the TEM room so I installed a thermometer. The temperature fluctuates by approx. 3°C (21.4-24.1°C) in the day or overnight. We have a Tecnai G20 and a powerful air conditioning system. I know that temperature should be as constant as possible but I don't know how much it is important. What inconvenients should I expect with such a temperature fluctuation? What are the specifications of this instrument?
Best regards, Stephane
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Sure the room temperature may have an effect on TEM performance over time, but that mainly relates to the power supplies and electronics. Provided your room temperature falls within the manufacturers specifications you should not have a problem. I have worked with instruments in hot countries well outside the recommendations and still not had problems.
If we consider the average exposure time, then temperature stability over that time is not a problem. There are many parts of the instrument that shield you from room temperature interference; general lens bulk, water cooling etc. You are more likely to suffer from magnetic field problems than any other in the modern day environment.
My only advice, if you worry about specimen stage stability, is to store the specimen rod IN THE MICROSCOPE! In this way the few seconds it will take to change the specimen will hardly change the rod temperature, and by the time you are settled in to record images, the rod will be back at "stage temperature". I have always thought it to be an operating error to store the specimen rod at room temperature, when it needs to be at specimen stage temperature. Working at very high resolution the most unstable unit in my experience is the specimen rod. Remember it is a directional object so a constant drift direction will be produced.
In the good old days, when we used a round specimen cartridge, the specimen drift problem was very rare, due to the heat transfer being in all directions rather than one!
Hope this helps?
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 03 September 2015 08:17 To: protrain-at-emcourses.com
Hi Alice!
What do you mean by "calculate"? Do you mean concentrations? EM is not an analytical method, you cannot get moles or mg of Ca per gram tissue or per cell or per mitochondrium with EM. Calcium is best quantified with mass spectrometry (ICP-MS and related) after isolating mitochondria, but there will be so few Calcium, I wonder if there is any method with enough sensitivity. If you just need relative comparisons you could use fluorescence labeling, like most people studying calcium in live cells.
Regards, Stephane
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Subject: [Microscopy] viaWWW:Biological sample preparation method for element analysis To: nizets2-at-yahoo.com Date: Thursday, August 20, 2015, 1:43 AM
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Email: fengxia.liang-at-med.nyu.edu Name: Alice Liang
Organization: NYULMC OCS Microscopy Core
Title-Subject: [Filtered] Biological sample preparation method for element analysis using EDX
Message: We have a project need to calculate calcium amount in the mitochondria of cultured cells. We tried once with SEM/EDX using thin section of routine TEM protocol of GA/OsO4/Epon with HEPES buffer, and result is not good. There are obvious some heavy mental contaminants, but we can't detect Calcium. Any suggestion?
Thanks, Alice NYULMC OCS Microscopy Core
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Hooke College of Applied Sciences, located in Westmont, IL, is offering a Transmission Electron Microscopy short course October 6-8, 2015. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.
For further TEM training details and registration information, please follow the link below: https://www.mccrone.com/transmission-electron-microscopy-tem-course
Best regards- __________________________________________________ Chris Gorman Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100
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Hello all, A researcher is about to undertake a TEM study of mammalian skeletal muscle. Several publications report using Sodium Cacodylate buffer as the carrier for the fixation steps. Is there a reason to be using this particular buffer in preference to a HEPES buffered saline or a phosphate buffer of some kind? I am not a fan of cacodylate, however if it is better for some aspects of muscle TEM imaging, I'd like to know what and why. Tissue will be immersion fixed, postfixed and embedded in an Epon type resin. Cryo is not an option for us at present.
Thanks in advance, Rosey
Rosey van Driel Manager, Transmission Electron Microscopy (TEM) Institute for Frontier Materials, GTP Research
Deakin University Geelong Waurn Ponds Campus, Locked Bag 20000, Geelong, VIC 3220 +61 3 52273117 Mobile 0417 399 630 rosey.vandriel-at-deakin.edu.au www.deakin.edu.au https://www.deakin.edu.au/research/facilities/electron-microscope/index.php Deakin University CRICOS Provider Code 00113B
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The affect on your system will depend on what you are trying to do with it. If you are using the instrument at moderate magnifications and not using STEM or EELS then you won't see too many problems. The change in temperature relative to your column and electronics temperature will induce alignment as well as specimen drift, for STEM, HRTEM, EELS etc this will cause you to lose performance and have issues with stability over extended periods.
You say you have a "powerful" a/c system. The issue may be it is TOO powerful, a/c systems are designed to generate a stable temperature with by operating continually with a certain load, if the load it too small it will constantly cycle on/off cooling causing the exact problem you have. You can test this by increasing the heat load in the room (say a few bar heaters) and seeing if you get an improvement. Not a very green long term solution but could help you justify a change. Another option is putting computer servers in the room as well, these generate lots of extra load. Another potential problem is the location of the temperature sensor relative to the room airflow, if it is directly in line with the output flow it could be over cooled, tricking the system into thinking it needs to shut off and on. A "ductsock" on the a/c out will take away any heterogeneous flow and minimise this problem.
Hope that's useful,
Matthew
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Hello Everyone, My management is interested in the inspection/optical systems offered by Nanotronics. Hopefully, we will be seeing their systems presently.
Does anyone have any experience with these systems?
Thanks!!!!
Frank Karl
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==============================Original Headers============================== 7, 28 -- From frank_karl-at-ardl.com Fri Sep 4 09:11:09 2015 7, 28 -- Received: from cal1-mh747b.smtproutes.com (cal1-mh747b.smtproutes.com [208.70.89.158]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t84EB8gD004052 7, 28 -- for {microscopy-at-microscopy.com} ; Fri, 4 Sep 2015 09:11:09 -0500 7, 28 -- X-Katharion-ID: 1441375840.35831.cal1-mh747 7, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by 7, 28 -- cal1-mh747.smtproutes.com [(192.69.16.69)] with ESMTP via TCP 7, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 04 Sep 2015 14:10:40 +0000 7, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 7, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Fri, 4 7, 28 -- Sep 2015 10:10:40 -0400 7, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 7, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 7, 28 -- {microscopy-at-microscopy.com} 7, 28 -- Date: Fri, 4 Sep 2015 10:10:39 -0400 7, 28 -- Subject: Who knows what lurks ..... 7, 28 -- Thread-Topic: Who knows what lurks ..... 7, 28 -- Thread-Index: AdDnG3nvtTglG/ldRO+zjLgf7hbi5g== 7, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772CE3BEA870-at-exchange2k7.ad.ardl.com} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- acceptlanguage: en-US 7, 28 -- Content-Type: text/plain; charset="us-ascii" 7, 28 -- MIME-Version: 1.0 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t84EB8gD004052 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Looking for optical adsorption and surface plasmons in the EDS
Message: I did not know EDS spectra showed optical absorption peaks...:-)
"The EDX spectrum, shown in Figure 8, reveals the clear elemental composition profile of the green synthesized Ag NPs. The intense signal at 3 keV strongly suggests that Ag was the major element, which has an optical absorption in this range due to the SPR [Reference: Mallikarjuna K, et al Phytochemical fabrication and characterization of silver nanoparticles by using pepper leaf broth. Arabian Journal of Chemistry. 2013] Notably, the other signals in the range of 0.0Â0.5 keV  one of which is very intense  represent the typical absorption of carbon and oxygen and thus indicates the presence of the plant extract (as a capping ligand) on the surfaces of the NPs." Khan M, et al. Green synthesis of silver nanoparticles mediated by Pulicaria glutinosa extract. International Journal of Nanomedicine. 2013;8:1507-1516.
"The appearance of an optical absorption peak at 2.80 keV in energy dispersive spectra further confirmed the presence of Pd-NPs" Dauthal P et al., Biosynthesis of palladium nanoparticles using delonix regia leaf extract and its catalytic activity for nitro-aromatics hydrogenation Ind. Eng. Chem. Res. 2013, 52, 18131Â 18139
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A series of peaks in the 2.5 - 3.5 keV range would most probably be Ag and Pd L shell x-rays. They are certainly not optical transitions.
Optical absorption peaks and plasmons are generally { 20 eV (with visible emission wavelengths { 4 eV). You will need high resolution EELS, or true optical spectroscopy to measure those.
The author's statements are attributing this to optical absorption are simply wrong and clearly the Journal's reviewer also did not understand the spectroscopy being employed.
Cheers,
Nestor
On Sep 4, 2015, at 6:19 PM CDT, {microscopy.listserver-at-gmail.com} {microscopy.listserver-at-gmail.com} wrote:
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The intense signal at 3 keV strongly suggests that Ag was the major element, } which has an optical absorption in this range due to the SPR [Reference: Mallikarjuna K, et al } Phytochemical fabrication and characterization of silver nanoparticles by using pepper leaf broth. } Arabian Journal of Chemistry. 2013] Notably, the other signals in the range of 0.00.5 keV one of } which is very intense represent the typical absorption of carbon and oxygen and thus indicates the } presence of the plant extract (as a capping ligand) on the surfaces of the NPs." } Khan M, et al. Green synthesis of silver nanoparticles mediated by Pulicaria glutinosa extract. } International Journal of Nanomedicine. 2013;8:1507-1516. } } "The appearance of an optical absorption peak at 2.80 keV in energy dispersive spectra further } confirmed the presence of Pd-NPs" } Dauthal P et al., Biosynthesis of palladium nanoparticles using delonix regia leaf extract and its } catalytic activity for nitro-aromatics hydrogenation Ind. Eng. Chem. Res. 2013, 52, 18131 18139 } } Login Host: 195.41.211.254 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. 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=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center NanoScience and Technology Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
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From ferguson651651615foxyx-at-gmail.com Fri Sep 4 19:05:43 2015 Return-Path: {ferguson651651615foxyx-at-gmail.com} Received: from gmail.com ([112.216.122.27]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t8505dXg029549 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 4 Sep 2015 19:05:41 -0500 Message-ID: {0706C4F0.FF1B69BC-at-gmail.com}
Say it ain't so! Those are not the optical absorptions due to SPR but the characteristic x-ray emissions. My carbon peaks show up around 0.27 keV, not zero. The fact that C and O are present does not mean that they are capping ligands, even if you would like them to be. It just means that they are around.
Someone really needs to get some better reviewers and editors. It's about bad enough to make me give up on lots of journals.
Warren Straszheim
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, September 04, 2015 6:21 PM To: Straszheim, Warren E [BIOTC]
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Title-Subject: [Filtered] Looking for optical adsorption and surface plasmons in the EDS
Message: I did not know EDS spectra showed optical absorption peaks...:-)
"The EDX spectrum, shown in Figure 8, reveals the clear elemental composition profile of the green synthesized Ag NPs. The intense signal at 3 keV strongly suggests that Ag was the major element, which has an optical absorption in this range due to the SPR [Reference: Mallikarjuna K, et al Phytochemical fabrication and characterization of silver nanoparticles by using pepper leaf broth. Arabian Journal of Chemistry. 2013] Notably, the other signals in the range of 0.0Â0.5 keV Â one of which is very intense represent the typical absorption of carbon and oxygen and thus indicates the presence of the plant extract (as a capping ligand) on the surfaces of the NPs." Khan M, et al. Green synthesis of silver nanoparticles mediated by Pulicaria glutinosa extract. International Journal of Nanomedicine. 2013;8:1507-1516.
"The appearance of an optical absorption peak at 2.80 keV in energy dispersive spectra further confirmed the presence of Pd-NPs" Dauthal P et al., Biosynthesis of palladium nanoparticles using delonix regia leaf extract and its catalytic activity for nitro-aromatics hydrogenation Ind. Eng. Chem. Res. 2013, 52, 18131Â 18139
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Thank you to all who responded to my query. This listserver is such a great resource!
First let me give you a little background. I switched from phosphate buffer to HEPES buffered saline back in the '90s, and had previously used phosphate buffer extensively (and cacodylate to a lesser extent). For morphology and for immunolabelling, HEPES buffered saline has been my buffer of choice for use with primary fixatives. I loved the way the cytoplasm retained density, and the morphology of tissue more closely resembled what could be achieved with cryofixation. Cacodylate seemed to result in washed out cytoplasm. (Almost all replies agreed with this.)
During my time working with tissues infected with viruses, about 10 years ago, I did another comparison between phosphate buffer, cacodylate buffer and HEPES buffered saline. For some tissues, use of cacodylate was beneficial as the washed out cytoplasm made the viral particles easier to find. In those cases we were not interested in the tissue, only the presence of viral particles for diagnostic purposes. (For morphology, HEPES was the outright winner.)
So I wondered if cacodylate persisted in the literature for skeletal muscle for a similar reason.
A few replies have been from researchers who have worked extensively with TEM of mammalian skeletal muscle, and use of phosphate buffer is common and successful. However, it seems cacodylate can give a more desirable morphology for this tissue type when compared with other buffers. Terry Robertson, who has extensive experience with TEM of skeletal muscle, has done buffer comparisons with this tissue. He says the results are far superior with cacodylate buffer for skeletal muscle. He writes that this advantage far outweighs the problems of working with it, and is probably because the high atomic number of the buffer means that it offers some contrast to organelles and membranes. Terry was also kind enough to provide a protocol and a reading list. Thanks Terry! Thanks again to all those who replied. There were many other good hints about processing skeletal muscle and other specimens. I always find it interesting to see the things others find advantageous in their work.
There are so many ways to prepare samples, and in the absence of cryofixation, each tissue requires some tweaking of the protocol for optimal results. Of course "optimal" depends entirely on what it is in the tissue you wish to visualize.
Due to the difficulties of ordering, storing, handling and controlling cacodylate, we might use HEPES in the pilot study. However, I have been convinced it may be worth the researcher's time and effort to work with cacodylate buffered fixative.
I do agree with Stephane Nizet that TEM imaging of skeletal muscle is one of the most enjoyable things! So pretty!
Best regards, Rosey
Rosey van Driel Manager, Transmission Electron Microscopy (TEM) Institute for Frontier Materials, GTP Research
Deakin University Geelong Waurn Ponds Campus, Locked Bag 20000, Geelong, VIC 3220 +61 3 52273117 rosey.vandriel-at-deakin.edu.au www.deakin.edu.au https://www.deakin.edu.au/research/facilities/electron-microscopy-facility Deakin University CRICOS Provider Code 00113B
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Seeking advise to water condensate inside the console (column and electronic electronics); it so happened that there seemed to have been a brief power interruption during my 10 day vacation and there was no alternative person to watch and report this incident.
When I returned and entered the lab, I found water all around the flooring and console. Power supply was on, the water chiller was operating and scope was inactive, and the PC had rebooted.
On seeking advise from the support center, I was asked to shut the chiller and cut the power to the machine. The approach seems to be to dry out all the system for 2 or three days and then attempt the diagnose the resulting damage.
What to expect. Please share your experience, If any of you have gone through a similar situation.
Thanks
Mohammed Yousuf PhD Central Laboratories Microscopy and micro-analysis facility Qatar University Doha, Qatar.
==============================Original Headers============================== 8, 46 -- From mdyousuf-at-qu.edu.qa Mon Sep 7 01:05:53 2015 8, 46 -- Received: from mx1.qu.edu.qa (Mx1.qu.edu.qa [86.36.67.16]) 8, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8765o7P021695 8, 46 -- for {microscopy-at-microscopy.com} ; Mon, 7 Sep 2015 01:05:53 -0500 8, 46 -- X-AuditID: 56244310-f79ec6d000001660-28-55ed293bd873 8, 46 -- Received: from MPPSDEXCHSRVR01.qu.edu.qa (Unknown_Domain [10.100.105.151]) 8, 46 -- by mx1.qu.edu.qa (Qatar University Mail Server) with SMTP id A5.65.05728.B392DE55; Mon, 7 Sep 2015 09:05:47 +0300 (AST) 8, 46 -- Received: from MPPSDEXCHSRVR03.qu.edu.qa (10.100.105.153) by 8, 46 -- MPPSDEXCHSRVR01.qu.edu.qa (10.100.105.151) with Microsoft SMTP Server (TLS) 8, 46 -- id 15.0.1076.9; Mon, 7 Sep 2015 09:05:09 +0300 8, 46 -- Received: from MPPSDEXCHSRVR03.qu.edu.qa ([fe80::d970:4541:3977:22d9]) by 8, 46 -- MPPSDEXCHSRVR03.qu.edu.qa ([fe80::d970:4541:3977:22d9%18]) with mapi id 8, 46 -- 15.00.1076.000; Mon, 7 Sep 2015 09:05:09 +0300 8, 46 -- From: Mohammed Yousuf {mdyousuf-at-qu.edu.qa} 8, 46 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 46 -- Subject: TEM - Tecnai TF20 - Water condensation problem 8, 46 -- Thread-Topic: TEM - Tecnai TF20 - Water condensation problem 8, 46 -- Thread-Index: AQHQ6TMmxH2PxGqfBEiRgG6K9QgMew== 8, 46 -- Date: Mon, 7 Sep 2015 06:05:08 +0000 8, 46 -- Message-ID: {60a5d4d9f19940eeb0d1876963692938-at-MPPSDEXCHSRVR03.qu.edu.qa} 8, 46 -- Accept-Language: en-US 8, 46 -- Content-Language: en-US 8, 46 -- X-MS-Has-Attach: 8, 46 -- X-MS-TNEF-Correlator: 8, 46 -- x-ms-exchange-transport-fromentityheader: Hosted 8, 46 -- x-originating-ip: [10.10.144.152] 8, 46 -- x-exclaimer-md-config: fb0dc0f7-341c-4801-99de-3789d9cf9cdf 8, 46 -- Content-Type: text/plain; charset="us-ascii" 8, 46 -- MIME-Version: 1.0 8, 46 -- X-Brightmail-Tracker: H4sIAAAAAAAAA02Sb0gTYRzHeW63eTt3ds6pTxakSwudmxqG5b8iX9SLlCAmZFCd7NqGc+pt 8, 46 -- E02CUUIxkSSRchgunUGpWGZY+IeaE9T+zRdJKJmoSMskykqDsO7xpu3d9/l+f5/n++O5I0Ty 8, 46 -- bkkMYTRbWc7MmJQSEid1xlvqrMQVberU3OFDf0bsIUfBiW91y9gpUERm61iTsZLlUnIvkIb6 8, 46 -- x+tY+auQKu+zOmAH1yUOICUgnQ6n3Y6AjoK+2R5ek4ScHgXw7536wGEAwNvtD8XCwQtgbU8D 8, 46 -- 5gAEIaGT4GBnAqIVdBbcmGkDSEfQGbCpZWRzREFnw2GvSRjRwPXeARHSOB0Prw3XipGm6BPQ 8, 46 -- vurf1IBfYm2iC0NaREfD6cVWTFiOhu7BtyJBR0L/woZY0KnwSccwjqogHQfnm3YLdhb8veqW 8, 46 -- CNckQ9fA94BWwXt3l0VCbTgcb17EG0CUM6jNGYQ4gxBnEOIC+ANAlValaSpsGlZn01QwvYD/ 8, 46 -- GIXxefRT0OjK9ACaAEoZ9d7xRSsXM5WW6lIPSCEwZSS1c/+KVh5WXKarNjAWw3nOZmItSgUV 8, 46 -- i2xq2y62mUo8ABIiPkrv4y+hdEz1JZYrEwAP2EXgymiq4NeyVk7rGStbwrLlLLeVHiQIJaTm 8, 46 -- 0J3hHKtnqy4aTdatmOdeK/iEDk6EQoyQeoCakPGts5sLWcqZUotRHwAjKHwf78q2XARNgBzC 8, 46 -- tT7Tjclxc5mZjYmmWhBJoxmDzbzdGhNFOSb9WvmOoADxn4GKf6oIagZRMv7X/l8np44jMzRg 8, 46 -- CtNq/hUVlDTXj7azMtbg7TKRK9ty0XyMHTTujVjYCLPuITtPv7np6EpqDxm64oxt7lyzF+Qn 8, 46 -- 9JKX5+eLSmr6Vnx5KtM4WRbFrh2gOriajCXlDbHf98N6JDH2nPrndP2Ytya9vz90Rf8y59jU 8, 46 -- yU9NXF75qPnFetuQ5itumfPJpM8L3VeXks9MFk62nr3/aEwVp83/+K77A6vELQYmLUnEWZh/ 8, 46 -- peFpZcADAAA= 8, 46 -- Content-Transfer-Encoding: 8bit 8, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8765o7P021695 ==============================End of - Headers==============================
From advertise.bz222ztuza-at-gmail.com Mon Sep 7 05:54:38 2015 Return-Path: {advertise.bz222ztuza-at-gmail.com} Received: from gmail.com ([121.254.237.37]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t87AsZTZ012807 for {microscopylistserverarchive5-at-microscopy.com} ; Mon, 7 Sep 2015 05:54:37 -0500 Message-ID: {D5014DDC.73763BF3-at-gmail.com}
Hi
I do not know if this will help but this is my experience?
Twice in my career I have had water pour all over an instrument, one from a bust tap creating a fountain in the microscope room, the other from a fire on upper floors, and the extinguisher water ending up on the microscope. I found that with clean water going all over the microscope, after a week of drying out, we had NO problems. With the fire water damage, waiting the same amount of time one circuit board suffered, this was due to the contamination of the board by media from the building itself being brought down onto the board surface. No matter how much cleaning I tried I could not persuade the board to run. It had to be replaced.
So clean water should not be a problem, dirty water probably is!
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: mdyousuf-at-qu.edu.qa [mailto:mdyousuf-at-qu.edu.qa] Sent: 07 September 2015 07:09 To: protrain-at-emcourses.com
Hello Fellow Microscopists -
The marketplace has decided that I should retire, so a fifty year accumulation of scientific journals is available to anyone with more space than I have:
Yes, I'm that guy, too: George Langford of georgesbasement.com.
The following titles are available:
Metallurgical Transactions in Series A and B, 1972 to 2002 with a few gaps. Acta Metallurgica from its inception (1953) through 1999, including its transmogrification to Acta Materialia, with only a few missing issues. Metallography from its inception (1968) to 1988 with some gaps. Transactions A.I.M.E. (metals section) from 1950 to 1969, mostly bound, but with some gaps. American Society for Metals Review of Metal Literature, 1964 through 1967. Progress in Metal Physics from 1949 to 1959, missing Vol's 5 & 6. A.S.T.M. yearly collections, many ... mostly metals related.
This is about a ton (1000 kg) of books & magazines.
I'm trying to place these in good homes. If you just want one issue from a year, it's $5 for the handling; larger quantities are free for the asking, but you must pick up with a sturdy vehicle or pay for FedEx delivery to the U.S.A. only; I'll supply the packing materials if shipment is necessary. I need space !
==============================Original Headers============================== 9, 28 -- From amenex-at-amenex.com Tue Sep 8 08:31:32 2015 9, 28 -- Received: from ld120.inmotionhosting.com (ld120.inmotionhosting.com [74.124.198.158]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t88DVWgH021760 9, 28 -- for {microscopy-at-microscopy.com} ; Tue, 8 Sep 2015 08:31:32 -0500 9, 28 -- Received: from localhost ([127.0.0.1]:62237 helo=ld120.inmotionhosting.com) 9, 28 -- by ld120.inmotionhosting.com with esmtpsa (TLSv1:RC4-SHA:128) 9, 28 -- (Exim 4.85) 9, 28 -- (envelope-from {amenex-at-amenex.com} ) 9, 28 -- id 1ZZIzO-00005S-KZ; Tue, 08 Sep 2015 06:31:30 -0700 9, 28 -- Received: from 72.78.196.173 ([72.78.196.173]) by ld120.inmotionhosting.com 9, 28 -- (Horde Framework) with HTTP; Tue, 08 Sep 2015 13:31:06 +0000 9, 28 -- Date: Tue, 08 Sep 2015 13:31:06 +0000 9, 28 -- Message-ID: {20150908133106.Horde.bu6RdNJJ90DscrJg4EyzSw9-at-ld120.inmotionhosting.com} 9, 28 -- From: amenex-at-amenex.com 9, 28 -- To: microscopy-at-microscopy.com 9, 28 -- Cc: "George Langford, Sc.D." {amenex-at-amenex.com} 9, 28 -- Subject: Metallurgical journals available in southeast pennsylvania 9, 28 -- User-Agent: Internet Messaging Program (IMP) H5 (6.1.4) 9, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Disposition: inline 9, 28 -- X-OutGoing-Spam-Status: No, score=-102.9 9, 28 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 9, 28 -- X-AntiAbuse: Primary Hostname - ld120.inmotionhosting.com 9, 28 -- X-AntiAbuse: Original Domain - microscopy.com 9, 28 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 9, 28 -- X-AntiAbuse: Sender Address Domain - amenex.com 9, 28 -- X-Get-Message-Sender-Via: ld120.inmotionhosting.com: authenticated_id: amenex-at-amenex.com ==============================End of - Headers==============================
I took quite some time to summarize the many answers I got concerning T° fluctuations in the TEM room. Unfortunately just before posting I unconsciously hit some cryptic shortcut key combination which loaded the previous web page, deleting my message at the same time. Thanks microsoft for making my life a little bit harder! I won't write it again but still I am grateful for the many answers, all useful.
Now to my next problem: I have discovered 1L (!!!) of uranyle acetate solution in a fridge, packed in alu foil. It is probably there for several years now (I guess around 10 years). I don't know who was crazy enough to do that but my heart bleeds at the thought of having to call a specialized company to dispose of the solution. Is there a way I can use it? Is there a hope than it still can be used (other than forcing the person who prepared it to drink it all at once)? Filtering the solution through a 0.22ľm filter will probably not be enough. Maybe I can centrifuge it? How fast and how long would I need to centrifuge to get rid of the finest precipitates? I don't have an ultracentrifuge! Should I just forget it, beg mother earth's pardon for the "inconvenience" and just make a new solution? Just write a new message to the List: uranyle acetate ready solution to give away. You pay the transport and the insurance ? :-)))))
Regards, Stephane
==============================Original Headers============================== 12, 36 -- From nizets2-at-yahoo.com Wed Sep 9 04:41:32 2015 12, 36 -- Received: from nm36-vm0.bullet.mail.ne1.yahoo.com (nm36-vm0.bullet.mail.ne1.yahoo.com [98.138.229.112]) 12, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t899fVs6007965 12, 36 -- for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2015 04:41:32 -0500 12, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1441791691; bh=K0BAW/PX223peaCzxatvCxHLhHud9DNaJigjLnbGChA=; h=Date:From:Subject:To:From:Subject; b=UbMjH39rNNlNpfjGr5kdqxflqBwNFrTMQj63GZ9s3Wz/s2qlN7ncUYahlutObfoWRZz5t+uM863k5Uh8+qAsCjER/Oi5VK2F+qNwbDSCxhBUW9+cJlzl9I9sPcKHiv88g8ozxtdO6uXCta8JANC2k9iEZuE+ao0ohmARi1op17oDDGWvZfFG6FnYV46fBC8FSEhvH8roF9P57/Z4eijNgUNSKG0vvChN8MttcvZO3/R+6z/bkOj0FmsDLnKbv89iYWf9uYpYBlb7AtJiiJWhiB9IxwqUH3l4xyqWTNDUWlTd1wM2M/dDunAwB/DKZjFAJZQ/lg7Jp5OF8VXsGxZh2A== 12, 36 -- Received: from [127.0.0.1] by nm36.bullet.mail.ne1.yahoo.com with NNFMP; 09 Sep 2015 09:41:31 -0000 12, 36 -- Received: from [98.138.100.116] by nm36.bullet.mail.ne1.yahoo.com with NNFMP; 09 Sep 2015 09:38:43 -0000 12, 36 -- Received: from [98.139.170.181] by tm107.bullet.mail.ne1.yahoo.com with NNFMP; 09 Sep 2015 09:38:43 -0000 12, 36 -- Received: from [98.139.212.217] by tm24.bullet.mail.bf1.yahoo.com with NNFMP; 09 Sep 2015 09:38:42 -0000 12, 36 -- Received: from [127.0.0.1] by omp1026.mail.bf1.yahoo.com with NNFMP; 09 Sep 2015 09:38:42 -0000 12, 36 -- X-Yahoo-Newman-Property: ymail-4 12, 36 -- X-Yahoo-Newman-Id: 942503.4143.bm-at-omp1026.mail.bf1.yahoo.com 12, 36 -- Received: (qmail 86888 invoked by uid 60001); 9 Sep 2015 09:38:42 -0000 12, 36 -- X-YMail-OSG: jA3FHy0VM1mQhqeWGTqXsL24oCR7f3eojoa7VZxnk9B_bx8 12, 36 -- UpWKi6oHArfiR2CAStg196ipE.hHOgN1nlRcuMocFc6xma0wOnL7wQWGLmIN 12, 36 -- nYfLYu_cLs7IZWcOlqmnTSizk0tPb9GS2iu2y8KMiJ_EqJ_lw04cT1VKh1JB 12, 36 -- E7ujlxWVXbXijz2k2qx7sVScZLtxXrflW9oWDIJAqDXUm1dcPLTw6M8seix8 12, 36 -- P4hU_weNoDg4IFN6cZk6Okjt5L.tI4vuwgnbyKc4MyChLS7coiOPNmY65zr6 12, 36 -- ZbnHIojvBqSuA2HJN6F7zjOJ7fXprJhIb4idVcZQdm2BQq.SO0aHDNxvRIKC 12, 36 -- P1W0BQHgycpfeb_xnyqYWuYTJ7bu8eaCLL5PcXON9q9ncAhmTu70dgJ_K4mj 12, 36 -- H7S_YWVli7EPptuSnD2tN4G5.OV_bsJNYkxi8dNMCKRZe80hDis3oZtk4zdE 12, 36 -- 6fAgXNlcH0cFugJwWvpLSEMj4BeYAQcYMM..VSJ_vO9CWRaUZRi1yLGLtxax 12, 36 -- oeb3RehP6iMS13rs0lPqT.7irpzBaSRHkhUbdCRiDKv0_ETwbN9zT1.3D_8S 12, 36 -- KQVAwrOk8bD0TT7c- 12, 36 -- Received: from [213.33.126.84] by web141703.mail.bf1.yahoo.com via HTTP; Wed, 09 Sep 2015 02:38:42 PDT 12, 36 -- X-Rocket-MIMEInfo: 002.001,RGVhciBsaXN0ZXJzLA0KDQpJIHRvb2sgcXVpdGUgc29tZSB0aW1lIHRvIHN1bW1hcml6ZSB0aGUgbWFueSBhbnN3ZXJzIEkgZ290IGNvbmNlcm5pbmcgVMKwIGZsdWN0dWF0aW9ucyBpbiB0aGUgVEVNIHJvb20uDQpVbmZvcnR1bmF0ZWx5IGp1c3QgYmVmb3JlIHBvc3RpbmcgSSB1bmNvbnNjaW91c2x5IGhpdCBzb21lIGNyeXB0aWMgc2hvcnRjdXQga2V5IGNvbWJpbmF0aW9uIHdoaWNoIGxvYWRlZCB0aGUgcHJldmlvdXMgd2ViIHBhZ2UsIGRlbGV0aW5nIG15IG1lc3NhZ2UgYXQgdGhlIHNhbWUgdGltZS4gVGgBMAEBAQE- 12, 36 -- X-Mailer: YahooMailBasic/651 YahooMailWebService/0.8.203.813 12, 36 -- Message-ID: {1441791522.38350.YahooMailBasic-at-web141703.mail.bf1.yahoo.com} 12, 36 -- Date: Wed, 9 Sep 2015 02:38:42 -0700 12, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} 12, 36 -- Subject: uranyl acetate stability, eliminating precipitates 12, 36 -- To: microscopy-at-microscopy.com 12, 36 -- MIME-Version: 1.0 12, 36 -- Content-Type: text/plain; charset=iso-8859-1 12, 36 -- Content-Transfer-Encoding: 8bit 12, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t899fVs6007965 ==============================End of - Headers==============================
Dear Fellow SEM and TEM microscopists, A typical energy dispersive X-ray spectrum recorded using a TEM or SEM has peaks corresponding to characteristic X-ray energies, superimposed on a Bremsstrahlung background. I notice there is a zero peak followed by some background with reasonable intensity and it appears differently by different detector manufacture in terms of intensity. I understand that ionisation process results in the characteristic X-ray peaks. But, what process or electron/matter interactions could be involved to give the X-rays at low energy up to 100eV? My detector registered X-ray counts there, it must have come from somewhere. I would be extremely grateful if you could give me some hints, or suggest some book chapters to read, or some papers to refer to.
Kind regards, Zhaoxia Zhou Dr Z Zhou Experimental Officer Loughborough Materials Characterisation Centre (LMCC) Department of Materials Loughborough University Leicestershire LE11 3TU
==============================Original Headers============================== 3, 56 -- From Z.Zhou-at-lboro.ac.uk Wed Sep 9 11:38:30 2015 3, 56 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 3, 56 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t89GcT1m004922 3, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Sep 2015 11:38:29 -0500 3, 56 -- Received: from [158.125.167.10] (helo=ITSCASH-3.lunet.lboro.ac.uk) 3, 56 -- by mta-1.lboro.ac.uk with esmtps (TLSv1:AES128-SHA:128) 3, 56 -- (Exim 4.80) 3, 56 -- id 1ZZiNp-00058J-7N 3, 56 -- for Microscopy-at-microscopy.com; Wed, 09 Sep 2015 17:38:05 +0100 3, 56 -- Received: from emea01-db3-obe.outbound.protection.outlook.com (213.199.154.80) 3, 56 -- by email.lboro.ac.uk (158.125.167.4) with Microsoft SMTP Server (TLS) id 3, 56 -- 14.3.235.1; Wed, 9 Sep 2015 17:37:57 +0100 3, 56 -- Received: from DB4PR04MB0623.eurprd04.prod.outlook.com (10.242.221.148) by 3, 56 -- DB4PR04MB0622.eurprd04.prod.outlook.com (10.242.221.147) with Microsoft SMTP 3, 56 -- Server (TLS) id 15.1.262.15; Wed, 9 Sep 2015 16:38:03 +0000 3, 56 -- Received: from DB4PR04MB0623.eurprd04.prod.outlook.com ([10.242.221.148]) by 3, 56 -- DB4PR04MB0623.eurprd04.prod.outlook.com ([10.242.221.148]) with mapi id 3, 56 -- 15.01.0262.011; Wed, 9 Sep 2015 16:38:03 +0000 3, 56 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} 3, 56 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 3, 56 -- Subject: EDX in SEM and TEM origin of X-rays 0-100eV energy 3, 56 -- Thread-Topic: EDX in SEM and TEM origin of X-rays 0-100eV energy 3, 56 -- Thread-Index: AdDrHd7IJAHadQT1R6W9nGac4nJi0g== 3, 56 -- Date: Wed, 9 Sep 2015 16:38:02 +0000 3, 56 -- Message-ID: {DB4PR04MB06239475312544488710B769C6520-at-DB4PR04MB0623.eurprd04.prod.outlook.com} 3, 56 -- Accept-Language: en-GB, en-US 3, 56 -- Content-Language: en-US 3, 56 -- X-MS-Has-Attach: 3, 56 -- X-MS-TNEF-Correlator: 3, 56 -- authentication-results: spf=none (sender IP is ) 3, 56 -- smtp.mailfrom=Z.Zhou-at-lboro.ac.uk; 3, 56 -- x-originating-ip: [2001:630:301:3064:a873:fb69:a801:a608] 3, 56 -- x-microsoft-exchange-diagnostics: 1;DB4PR04MB0622;5:nMIq0KgW0YbIDyOFfmCfERsI4hEWyJMMA4YZpYlwnDm3KKdf73vffHWnSg7BXDpZDuwnaskKGfLItEDDdVhUpTAZrvmXgPCCDU4FnmVY5utYgSjoxzytmWNTkElQri9FXC9tJ5NvjJEXHXa2+9ZrLQ==;24:B0ZvUwvrJ12VYki9xdeNZHrAGY6UO6qT2HQBlypXd0cU4FgWuO7gTJEAXlVxhmzjtLYEmH5oWtBFyIxvmv9Nfbyqjc2wr9IlbeFYxdzRddQ=;20:nj1fvrKEa7uTHxOxH1kNsBxssQRyjwsVmAwXM+atySYyVOpF+mgFgHtXDc9MXFniE7mzkab9qSEA6u8+tlwEdg== 3, 56 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:DB4PR04MB0622; 3, 56 -- x-microsoft-antispam-prvs: {DB4PR04MB06225AFC1DA52F6032C0519EC6520-at-DB4PR04MB0622.eurprd04.prod.outlook.com} 3, 56 -- x-exchange-antispam-report-test: UriScan:; 3, 56 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(8121501046)(5005006)(3002001);SRVR:DB4PR04MB0622;BCL:0;PCL:0;RULEID:;SRVR:DB4PR04MB0622; 3, 56 -- x-forefront-prvs: 0694C54398 3, 56 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(71364002)(189002)(5001830100001)(102836002)(46102003)(74482002)(450100001)(87936001)(77156002)(74316001)(101416001)(2351001)(86362001)(2501003)(229853001)(33656002)(50986999)(77096005)(97736004)(5004730100002)(5002640100001)(54356999)(81156007)(11100500001)(5007970100001)(62966003)(68736005)(107886002)(105586002)(10400500002)(5001920100001)(5001860100001)(40100003)(2900100001)(189998001)(92566002)(76576001)(122556002)(64706001)(5001960100002)(5003600100002)(4001540100001)(106356001)(110136002)(3826002);DIR:OUT;SFP:1102;SCL:1;SRVR:DB4PR04MB0622;H:DB4PR04MB0623.eurprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 3, 56 -- received-spf: None (protection.outlook.com: lboro.ac.uk does not designate 3, 56 -- permitted sender hosts) 3, 56 -- spamdiagnosticoutput: 1:23 3, 56 -- spamdiagnosticmetadata: NSPM 3, 56 -- Content-Type: text/plain; charset="utf-8" 3, 56 -- MIME-Version: 1.0 3, 56 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 09 Sep 2015 16:38:03.0230 3, 56 -- (UTC) 3, 56 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 3, 56 -- X-MS-Exchange-CrossTenant-id: cf264fc0-aeb8-449f-9054-82ce4454084b 3, 56 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DB4PR04MB0622 3, 56 -- X-OriginatorOrg: lboro.ac.uk 3, 56 -- X-Scan-Signature: 6c534765df601ba4d0e3a26d153e2019 3, 56 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 3, 56 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Wed, 09 Sep 2015 17:38:28 +0100 3, 56 -- Content-Transfer-Encoding: 8bit 3, 56 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t89GcT1m004922 ==============================End of - Headers==============================
The fact that this zero peak appears different from detector to detector is your clue to the origin, a peak or peaks in this region is almost certainly an instrumental effect, and NOT x-rays. The various components in the detector and processing electronics generate some electronic noise which is digitized along with the true x-rays generated from the sample. Most pulse processors have a set of discriminators that are set on installation to minimize the amount of these effect, but cannot eliminate them entirely. This is true even if you have a recent "digital" pulse Processor--there will still be some software settings that act to reduce the zero noise peak.
Every manufacturer does this a bit differently hence the difference you see. If you can modify the discriminator setting on your unit try tweaking then a bit and you will see some interesting stuff. Proper discriminator settings are critical to getting good light element x-ray detection with proper peak shapes and peak positions.
Regards Jon
Jon J McCarthy, Ph. D. Senior Scientist- Emeritus and Researcher Wisconsin Materials Institute College of Engineering University of Wisconsin-Madison Room 246, Materials Science and Engineering Bldg. 608-890-3134 Office 608-345-6134 Cell jjmccarthy-at-wisc.edu
-----Original Message----- X-from: Z.Zhou-at-lboro.ac.uk [mailto:Z.Zhou-at-lboro.ac.uk] Sent: Wednesday, September 9, 2015 12:01 PM To: Jon Mccarthy {jjmccarthy-at-wisc.edu}
Dear Fellow SEM and TEM microscopists, A typical energy dispersive X-ray spectrum recorded using a TEM or SEM has peaks corresponding to characteristic X-ray energies, superimposed on a Bremsstrahlung background. I notice there is a zero peak followed by some background with reasonable intensity and it appears differently by different detector manufacture in terms of intensity. I understand that ionisation process results in the characteristic X-ray peaks. But, what process or electron/matter interactions could be involved to give the X-rays at low energy up to 100eV? My detector registered X-ray counts there, it must have come from somewhere. I would be extremely grateful if you could give me some hints, or suggest some book chapters to read, or some papers to refer to.
Kind regards, Zhaoxia Zhou Dr Z Zhou Experimental Officer Loughborough Materials Characterisation Centre (LMCC) Department of Materials Loughborough University Leicestershire LE11 3TU
==============================Original Headers============================== 3, 56 -- From Z.Zhou-at-lboro.ac.uk Wed Sep 9 11:38:30 2015 3, 56 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 3, 56 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t89GcT1m004922 3, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Sep 2015 11:38:29 -0500 3, 56 -- Received: from [158.125.167.10] (helo=ITSCASH-3.lunet.lboro.ac.uk) 3, 56 -- by mta-1.lboro.ac.uk with esmtps (TLSv1:AES128-SHA:128) 3, 56 -- (Exim 4.80) 3, 56 -- id 1ZZiNp-00058J-7N 3, 56 -- for Microscopy-at-microscopy.com; Wed, 09 Sep 2015 17:38:05 +0100 3, 56 -- Received: from emea01-db3-obe.outbound.protection.outlook.com (213.199.154.80) 3, 56 -- by email.lboro.ac.uk (158.125.167.4) with Microsoft SMTP Server (TLS) id 3, 56 -- 14.3.235.1; Wed, 9 Sep 2015 17:37:57 +0100 3, 56 -- Received: from DB4PR04MB0623.eurprd04.prod.outlook.com (10.242.221.148) by 3, 56 -- DB4PR04MB0622.eurprd04.prod.outlook.com (10.242.221.147) with Microsoft SMTP 3, 56 -- Server (TLS) id 15.1.262.15; Wed, 9 Sep 2015 16:38:03 +0000 3, 56 -- Received: from DB4PR04MB0623.eurprd04.prod.outlook.com ([10.242.221.148]) by 3, 56 -- DB4PR04MB0623.eurprd04.prod.outlook.com ([10.242.221.148]) with mapi id 3, 56 -- 15.01.0262.011; Wed, 9 Sep 2015 16:38:03 +0000 3, 56 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} 3, 56 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 3, 56 -- Subject: EDX in SEM and TEM origin of X-rays 0-100eV energy 3, 56 -- Thread-Topic: EDX in SEM and TEM origin of X-rays 0-100eV energy 3, 56 -- Thread-Index: AdDrHd7IJAHadQT1R6W9nGac4nJi0g== 3, 56 -- Date: Wed, 9 Sep 2015 16:38:02 +0000 3, 56 -- Message-ID: {DB4PR04MB06239475312544488710B769C6520-at-DB4PR04MB0623.eurprd04.prod.outlook.com} 3, 56 -- Accept-Language: en-GB, en-US 3, 56 -- Content-Language: en-US 3, 56 -- X-MS-Has-Attach: 3, 56 -- X-MS-TNEF-Correlator: 3, 56 -- authentication-results: spf=none (sender IP is ) 3, 56 -- smtp.mailfrom=Z.Zhou-at-lboro.ac.uk; 3, 56 -- x-originating-ip: [2001:630:301:3064:a873:fb69:a801:a608] 3, 56 -- x-microsoft-exchange-diagnostics: 1;DB4PR04MB0622;5:nMIq0KgW0YbIDyOFfmCfERsI4hEWyJMMA4YZpYlwnDm3KKdf73vffHWnSg7BXDpZDuwnaskKGfLItEDDdVhUpTAZrvmXgPCCDU4FnmVY5utYgSjoxzytmWNTkElQri9FXC9tJ5NvjJEXHXa2+9ZrLQ==;24:B0ZvUwvrJ12VYki9xdeNZHrAGY6UO6qT2HQBlypXd0cU4FgWuO7gTJEAXlVxhmzjtLYEmH5oWtBFyIxvmv9Nfbyqjc2wr9IlbeFYxdzRddQ=;20:nj1fvrKEa7uTHxOxH1kNsBxssQRyjwsVmAwXM+atySYyVOpF+mgFgHtXDc9MXFniE7mzkab9qSEA6u8+tlwEdg== 3, 56 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:DB4PR04MB0622; 3, 56 -- x-microsoft-antispam-prvs: {DB4PR04MB06225AFC1DA52F6032C0519EC6520-at-DB4PR04MB0622.eurprd04.prod.outlook.com} 3, 56 -- x-exchange-antispam-report-test: UriScan:; 3, 56 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(8121501046)(5005006)(3002001);SRVR:DB4PR04MB0622;BCL:0;PCL:0;RULEID:;SRVR:DB4PR04MB0622; 3, 56 -- x-forefront-prvs: 0694C54398 3, 56 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(71364002)(189002)(5001830100001)(102836002)(46102003)(74482002)(450100001)(87936001)(77156002)(74316001)(101416001)(2351001)(86362001)(2501003)(229853001)(33656002)(50986999)(77096005)(97736004)(5004730100002)(5002640100001)(54356999)(81156007)(11100500001)(5007970100001)(62966003)(68736005)(107886002)(105586002)(10400500002)(5001920100001)(5001860100001)(40100003)(2900100001)(189998001)(92566002)(76576001)(122556002)(64706001)(5001960100002)(5003600100002)(4001540100001)(106356001)(110136002)(3826002);DIR:OUT;SFP:1102;SCL:1;SRVR:DB4PR04MB0622;H:DB4PR04MB0623.eurprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 3, 56 -- received-spf: None (protection.outlook.com: lboro.ac.uk does not designate 3, 56 -- permitted sender hosts) 3, 56 -- spamdiagnosticoutput: 1:23 3, 56 -- spamdiagnosticmetadata: NSPM 3, 56 -- Content-Type: text/plain; charset="utf-8" 3, 56 -- MIME-Version: 1.0 3, 56 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 09 Sep 2015 16:38:03.0230 3, 56 -- (UTC) 3, 56 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 3, 56 -- X-MS-Exchange-CrossTenant-id: cf264fc0-aeb8-449f-9054-82ce4454084b 3, 56 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DB4PR04MB0622 3, 56 -- X-OriginatorOrg: lboro.ac.uk 3, 56 -- X-Scan-Signature: 6c534765df601ba4d0e3a26d153e2019 3, 56 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 3, 56 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Wed, 09 Sep 2015 17:38:28 +0100 3, 56 -- Content-Transfer-Encoding: 8bit 3, 56 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t89GcT1m004922 ==============================End of - Headers==============================
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From mike.sfsd4f564s6df45dshyioa-at-gmail.com Wed Sep 9 14:30:23 2015 Return-Path: {mike.sfsd4f564s6df45dshyioa-at-gmail.com} Received: from gmail.com ([220.241.216.221]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t89JUJWe029429 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 9 Sep 2015 14:30:21 -0500 Message-ID: {299F208C.1DE7064E-at-gmail.com}
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Email: jefbettini-at-gmail.com Name: Jefferson Bettini
Organization: CNPEM/LNNano
Title-Subject: [Filtered] 6th TEM Summer School in Brazil
Message: LNNano receives applications for the 6th TEM Summer School
Candidates may apply until August 31st. 100 participants are expected to attend the school.
Conducted every two years the already established Transmission Electron Microscopy (TEM) Summer School will receive applications for its sixth edition until August 31st. The event will be held at the Brazilian Nanotechnology National Laboratory (LNNano), located in the Brazilian Center for Research in Energy and Materials (CNPEM) campus, Campinas - Brazil, between January 11 and 29, 2016.
This school aims to contribute to the advanced training of graduate students and researchers in the fields of engineering, materials science, physics, chemistry and related fields from academic and industrial communities, in Brazil and abroad, on theoretical and practical concepts of TEM techniques for materials characterization. It is a three week course where the first two weeks are dedicated to basic and advanced theoretical classes and the third one will be focused on practical classes using the Electron Microscopy facilities available at LNNano.
The courses will be taught by leading researchers in the field of electron microscopy associated to important research and educational institutions of Brazil and oversees. See all speakers for the 6th edition.
Official invitation from the Organizing Committee
Every candidate must fill out the application form, attaching an updated CV, Graduate transcripts, a summary of research project justifying the use of TEM techniques, an essay justifying why is important for you to attend the school, an abstract of partial or complete analysis of your own scientific research (using TEM) to present as poster, and a recommendation letter. Check How to participate.
The 6th TEM Summer School is organized by the Electron Microscopy Laboratory (LME/LNNano) with the FAPESP support. For further information please visit: http://pages.cnpem.br/temsummerschool/
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Email: slkodjie-at-dow.com Name: Stephen Kodjie
Organization: Dow Chemical
Title-Subject: [Filtered] Mettler Toledo FP80 Temperature Controller
Message: I have a Mettler Toledo FP80 temperature controller but would need a manual in order to operate it. The Mettler Toledo website indicate that this product has been phased out since 1992. Does anyone have copy of the manual they can share? Either electronic format or hardcopy would be much appreciated.
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University of Connecticut, Institute of Materials Science Position in X-Ray Diffraction
The Institute of Materials Science (IMS) at UConn is an interdisciplinary center with the threefold mission of fostering education, research and outreach in all areas of the materials sciences. The X-ray Laboratory is a user facility which houses the main X-ray research instruments for the IMS including: single crystal diffraction, powder diffraction, scattering, and imaging. (see: http://www.ims.uconn.edu/research-facilities/x-ray-diffraction/).
There is an opening in the Laboratory for an X-ray specialist. The appointee will be involved in a range of academic and industrial projects, and will assist in the operation of the Laboratory including: offering short courses, performing routine maintenance, and training/assisting users of the instruments. Candidates should hold a higher degree (MS or Ph.D.) in Materials Science or a related discipline and must have extensive hands-on experience in X-ray diffraction / scattering/ imaging. Experience in maintenance of instrumentation would also be beneficial. This is a fixed-term appointment (one year in the first instance) and is available from October 1st. Screening of the applications will begin immediately and will continue until the post is filled. Applications from under-represented groups, including minorities, women, and persons with disabilities are encouraged.
Interested candidates should send a curriculum vitae and the names of at least three referees with postal addresses, telephone numbers and Email addresses to: Prof. Steve Suib, Director, Institute of Materials Science, University of Connecticut, 97 North Eagleville Road, Unit 3136, Storrs, CT 06269-3136 USA. Email: steven.suib-at-uconn.edu
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Email: 13qw9-at-queensu.ca Name: Jason
Title-Subject: [Filtered] 30KV fails atuomatically in FEI Nova NanoSEM450
Message: hello everybody, could anyone give some suggestions to our current problem? We are using a FEI Nova NanoSEM450. Whenever we use the 30KV, it only last for around 5 hours before fails. And if we turn on the beam again using 30KV, it will turn off instantly again. But it allows us to use the next day, and of course still only 5 hours. The 20KV and lower work fine. The technician checked everything but didn't find any problem...
Thank you in advance for your help!
Jason
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We had a similar problem with a Philips CM200 that would arc at 200 kV after several hours. Still had the problem after replacing the entire emission chamber. It turned out to be a DC power supply that regulated the high voltage. A 15 volt DC supply would start losing voltage causing the high voltage tank to increase to 220 kV plus then arc. It took a rookie service engineer, Ken Hurst, to setup a laptop to read all of the DC power supplies to find the one causing the problem.
microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: 13qw9-at-queensu.ca } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both 13qw9-at-queensu.ca as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: 13qw9-at-queensu.ca } Name: Jason } } Title-Subject: [Filtered] 30KV fails atuomatically in FEI Nova NanoSEM450 } } Message: hello everybody, could anyone give some suggestions to our current problem? We are using a } FEI Nova NanoSEM450. Whenever we use the 30KV, it only last for around 5 hours before fails. And if } we turn on the beam again using 30KV, it will turn off instantly again. But it allows us to use the } next day, and of course still only 5 hours. The 20KV and lower work fine. The technician checked } everything but didn't find any problem... } } Thank you in advance for your help! } } Jason } } Login Host: 130.15.32.234 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } }
I'm getting an increase in researchers coming to me wanting to see autophagosomes (the last few years it seems that autophagy is becoming quite a popular topic). Getting images of autophagosomes is not a problem. The problem I am having with researchers, is getting them to understand that you cannot quantify the presence of the autophagosomes by simply counting the ones you see in a few sections. When I try to explain about stereology methods we could use to quantify the autophagosomes I lose them, in addition I keep getting presented with papers they have read in which the quantitative methods being used are clearly wrong. I would like to hear from others who are dealing with similar requests and how are you handling these requests. In particular anyone who is involved in quantifying autophagosomes by using TEM. As always thanks in advance, all help is appreciated.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Mon Sep 14 09:52:37 2015 7, 37 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8EEqbNd030104 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Sep 2015 09:52:37 -0500 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id 9C30937CDF3 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Sep 2015 09:42:45 -0500 (CDT) 7, 37 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id 660AF37CDEF 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Sep 2015 09:42:44 -0500 (CDT) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 37 -- 14.03.0235.001; Mon, 14 Sep 2015 09:52:35 -0500 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Quantification of autophagosomes using TEM 7, 37 -- Thread-Topic: Quantification of autophagosomes using TEM 7, 37 -- Thread-Index: AdDu+xnCXMNDi18wS3WWYZ3kbX545A== 7, 37 -- Date: Mon, 14 Sep 2015 14:52:31 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0747379FE-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: 3541dcc2-eb29-4e04-8e5a-6f0b69339c56 7, 37 -- X-VPM-HOST: zixvpm02.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 9d7c9ecf-dd0a-4cb7-b69f-d9a647655caa 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8EEqbNd030104 ==============================End of - Headers==============================
Anyone have advice about jet thinning aluminum alloys?
Thinking of trying it, but need ideas. Anyone doing it or know someone who is?
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 15, 38 -- From prvs=169384dedb=jkrupp-at-deltacollege.edu Tue Sep 15 13:11:52 2015 15, 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.175.111]) 15, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t8FIBqw8026845 15, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 13:11:52 -0500 15, 38 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 15, 38 -- by localhost (Email Security Appliance) with SMTP id 425A03F7793_5F85F1CB 15, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 18:10:36 +0000 (GMT) 15, 38 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) 15, 38 -- by mailin.deltacollege.edu (Sophos Email Appliance) with SMTP id 2DC652BE117_5F85F1CF 15, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 18:10:36 +0000 (GMT) 15, 38 -- Received: from localhost (localhost [127.0.0.1]) 15, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 632DBF801242 15, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 11:11:48 -0700 (PDT) 15, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 15, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 15, 38 -- with ESMTP id 1ixGwDxRJVDk for {Microscopy-at-microscopy.com} ; 15, 38 -- Tue, 15 Sep 2015 11:11:48 -0700 (PDT) 15, 38 -- Received: from localhost (localhost [127.0.0.1]) 15, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 09BA5F85AD54 15, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 11:11:48 -0700 (PDT) 15, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 15, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 15, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 15, 38 -- with ESMTP id NY3Pbaugc7GU for {Microscopy-at-microscopy.com} ; 15, 38 -- Tue, 15 Sep 2015 11:11:47 -0700 (PDT) 15, 38 -- Received: from [10.249.4.9] (unknown [10.249.4.9]) 15, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id E1FB0F801242 15, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 11:11:47 -0700 (PDT) 15, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 15, 38 -- Content-Type: text/plain; charset=us-ascii 15, 38 -- Subject: Jet thin Al? 15, 38 -- Date: Tue, 15 Sep 2015 11:11:48 -0700 15, 38 -- Message-Id: {01AAF820-19B5-470B-9E9F-662E380556C8-at-deltacollege.edu} 15, 38 -- To: Microscopy-at-microscopy.com 15, 38 -- Mime-Version: 1.0 (Apple Message framework v1085) 15, 38 -- X-Mailer: Apple Mail (2.1085) 15, 38 -- Content-Transfer-Encoding: 8bit 15, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8FIBqw8026845 ==============================End of - Headers==============================
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} Ask a Microscopist } } } The following Ask a Microscopist form submission was received from your website. } } } Name: Connie Cummings } } School: MSA Member } } Grade/Education Level: Graduate } } Location: Durham, NC US } } Email: ultrapathimaging-at-gmail.com } } Subject: Sampling } } Your Question: An ongoing hair-pulling dispute between me, the microscopist, and almost every person doing research: Why did you take so many photomicrographs for each sample? Wouldn't say two or three photographs be just as good as the 5 to 10 you took. I, the investigation, still can't get over you embedding 6 blocks and sectioning all those blocks as semi-things! } HOW DO YOU HANDLE THIS SCENARIO? HOW DO YOU DEMONSTRATE THAT MORE IN EM IS ACTUALLY BETTER! }
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Tue Sep 15 13:55:51 2015 5, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8FItoHZ016191 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 13:55:50 -0500 5, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 5, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8FItm5T018670 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 14:55:49 -0400 5, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 5, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.248.2; Tue, 15 Sep 2015 14:55:48 -0400 5, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.248.2; Tue, 15 Sep 2015 14:55:48 -0400 5, 32 -- Message-ID: {55F869B4.7010204-at-cmich.edu} 5, 32 -- Date: Tue, 15 Sep 2015 14:55:48 -0400 5, 32 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: micro {microscopy-at-microscopy.com} 5, 32 -- Subject: Re: Ask a Microscopist 5, 32 -- References: {535726763.1684.1442338747552.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 5, 32 -- In-Reply-To: {535726763.1684.1442338747552.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 5, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit 5, 32 -- X-Originating-IP: [141.209.2.100] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 02PhuTNja - 8c77604ccdfa - 20150915 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
} } Your Question: An ongoing hair-pulling dispute between me, the } } microscopist, and almost every person doing research: Why did you take } } so many photomicrographs for each sample? Wouldn't say two or three } } photographs be just as good as the 5 to 10 you took. I, the } } investigation, still can't get over you embedding 6 blocks and } } sectioning all those blocks as semi-things! HOW DO YOU HANDLE THIS } } SCENARIO? HOW DO YOU DEMONSTRATE THAT MORE IN EM IS ACTUALLY BETTER!
Hahahaa. Yeah. Well, I draw them pictures (I always draw pictures, as if for a child) of their sample size. For TEM, I draw a cell. Then I draw the nucleus and maybe a few other organelles. Then I draw the object of interest, particularly if they are looking for a virus. Then I draw a line through the cell, representing a 60-80 nm section, which clearly misses any of the object of interest. Then I draw a bunch more parallel lines, representing lots more sections, still missing the object of interest. If it's a cell pellet, I draw more cells, but show that, unless there are a lot of cells and they are ALL showing the object of interest, my paltry 1mm x 2mm x 60nm section is going to miss it. If it's a tissue, same problem, probably worse. Explain sample size.
For semi-thins, same problem but at a slightly different scale. I just serial-sectioned a small marine organism for somebody who had a pre-conceived notion of how something worked based on a couple of random sections taken from a paraffin block ages ago. And gave a talk about it. The serial resin sections clearly showed something else was going on altogether, and then the intermittant ultrathins blew it all out of the water. (I love my job, teehee.)
This is why I really encourage investigators to come and do their own microscopy, so they have a better idea of the context. If they don't, I take a zillion micrographs and send them all. I hate just giving them a few select micrographs that may or may not reflect my bias (me, biased?).
We can rant together!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 8, 23 -- From tina-at-pbrc.hawaii.edu Tue Sep 15 14:28:48 2015 8, 23 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8FJSlwG004943 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 14:28:47 -0500 8, 23 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 8, 23 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id t8FJSghk007867 8, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 8, 23 -- Tue, 15 Sep 2015 09:28:42 -1000 (HST) 8, 23 -- Received: from localhost (tina-at-localhost) 8, 23 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id t8FJSg7l007863; 8, 23 -- Tue, 15 Sep 2015 09:28:42 -1000 (HST) 8, 23 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 8, 23 -- Date: Tue, 15 Sep 2015 09:28:41 -1000 (HST) 8, 23 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 8, 23 -- X-X-Sender: tina-at-b1000 8, 23 -- To: ultrapathimaging-at-gmail.com, 8, 23 -- Microscopy Listserver {Microscopy-at-microscopy.com} 8, 23 -- Subject: Re: Sampling, Ask a Microscopist 8, 23 -- In-Reply-To: {201509151857.t8FIv1E8017316-at-ns.microscopy.com} 8, 23 -- Message-ID: {Pine.GSO.4.64.1509150913190.6753-at-b1000} 8, 23 -- References: {201509151857.t8FIv1E8017316-at-ns.microscopy.com} 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
If you are digital, the memory for the images is PRACTICALLY FREE! Any professional photographer these days just shoots gazzilions of pictures. Been to a wedding lately? The photog just sits there, clicking away. My last vacation, I took 1,354 photos in 2 weeks. After I got home, I realized it was not enough. Experiments are expensive. Specimen prep is expensive. Once you have the object to photograph, take as many photos as you can!
John Mardinly, ASU
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Tuesday, September 15, 2015 12:06 PM To: John Mardinly
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} Ask a Microscopist } } } The following Ask a Microscopist form submission was received from your website. } } } Name: Connie Cummings } } School: MSA Member } } Grade/Education Level: Graduate } } Location: Durham, NC US } } Email: ultrapathimaging-at-gmail.com } } Subject: Sampling } } Your Question: An ongoing hair-pulling dispute between me, the microscopist, and almost every person doing research: Why did you take so many photomicrographs for each sample? Wouldn't say two or three photographs be just as good as the 5 to 10 you took. I, the investigation, still can't get over you embedding 6 blocks and sectioning all those blocks as semi-things! } HOW DO YOU HANDLE THIS SCENARIO? HOW DO YOU DEMONSTRATE THAT MORE IN EM IS ACTUALLY BETTER! }
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Tue Sep 15 13:55:51 2015 5, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8FItoHZ016191 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 13:55:50 -0500 5, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 5, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8FItm5T018670 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 14:55:49 -0400 5, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 5, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.248.2; Tue, 15 Sep 2015 14:55:48 -0400 5, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.248.2; Tue, 15 Sep 2015 14:55:48 -0400 5, 32 -- Message-ID: {55F869B4.7010204-at-cmich.edu} 5, 32 -- Date: Tue, 15 Sep 2015 14:55:48 -0400 5, 32 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: micro {microscopy-at-microscopy.com} 5, 32 -- Subject: Re: Ask a Microscopist 5, 32 -- References: {535726763.1684.1442338747552.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 5, 32 -- In-Reply-To: {535726763.1684.1442338747552.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 5, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit 5, 32 -- X-Originating-IP: [141.209.2.100] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 02PhuTNja - 8c77604ccdfa - 20150915 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 14, 37 -- From John.Mardinly-at-asu.edu Tue Sep 15 14:50:39 2015 14, 37 -- Received: from bcnetw2.asu.edu (bcnetw2.asu.edu [149.169.2.72]) 14, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8FJodEk025625 14, 37 -- for {Microscopy-at-Microscopy.com} ; Tue, 15 Sep 2015 14:50:39 -0500 14, 37 -- X-ASG-Debug-ID: 1442346637-064f8d0b8d66ff0001-FOsErg 14, 37 -- Received: from exhubw02.asurite.ad.asu.edu (exhubw02.asurite.ad.asu.edu [129.219.4.200]) by bcnetw2.asu.edu with ESMTP id 2XLffz8QGNXLZNLc (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Tue, 15 Sep 2015 12:50:37 -0700 (MST) 14, 37 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 14, 37 -- X-Barracuda-Apparent-Source-IP: 129.219.4.200 14, 37 -- X-ASG-Whitelist: Client 14, 37 -- Received: from EXMBT01.asurite.ad.asu.edu ([129.219.103.44]) by 14, 37 -- exhubw02.asurite.ad.asu.edu ([129.219.4.200]) with mapi id 14.03.0210.002; 14, 37 -- Tue, 15 Sep 2015 12:50:37 -0700 14, 37 -- From: John Mardinly {John.Mardinly-at-asu.edu} 14, 37 -- To: "ultrapathimaging-at-gmail.com" {ultrapathimaging-at-gmail.com} , 14, 37 -- "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 14, 37 -- Subject: FW: [Microscopy] Re: Ask a Microscopist 14, 37 -- Thread-Topic: [Microscopy] Re: Ask a Microscopist 14, 37 -- X-ASG-Orig-Subj: FW: [Microscopy] Re: Ask a Microscopist 14, 37 -- Thread-Index: AQHQ7+mLo/aTxc+7tkuMeXgvylmvpJ49/gFg 14, 37 -- Date: Tue, 15 Sep 2015 19:50:36 +0000 14, 37 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9402478E0ED-at-exmbt01.asurite.ad.asu.edu} 14, 37 -- Accept-Language: en-US 14, 37 -- Content-Language: en-US 14, 37 -- X-MS-Has-Attach: 14, 37 -- X-MS-TNEF-Correlator: 14, 37 -- x-originating-ip: [129.219.103.4] 14, 37 -- Content-Type: text/plain; charset="us-ascii" 14, 37 -- MIME-Version: 1.0 14, 37 -- X-Barracuda-Connect: exhubw02.asurite.ad.asu.edu[129.219.4.200] 14, 37 -- X-Barracuda-Start-Time: 1442346637 14, 37 -- X-Barracuda-Encrypted: AES128-SHA 14, 37 -- X-Barracuda-URL: https://149.169.2.72:443/cgi-mod/mark.cgi 14, 37 -- Received-SPF: neutral (asu.edu: 129.219.103.44 is neither permitted nor denied by domain of john.mardinly-at-asu.edu) 14, 37 -- X-Virus-Scanned: by bsmtpd at asu.edu 14, 37 -- X-Barracuda-BRTS-Status: 1 14, 37 -- Content-Transfer-Encoding: 8bit 14, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8FJodEk025625 ==============================End of - Headers==============================
One word: statistics. If that doesn't work, ask them what a sample is. The cell within which the Thing is hidden? So how many cells need to be sampled? From how many different tissues/organisms because the cells vary among tissues and organisms. The Thing, because they need to know the Thing's ultrastructure, which may vary by the local micro-environment? How many Things from how many different micro-environments need to be sampled? Perhaps it's the tissue, so how many bits of tissue need to be sampled? How many sections have to be cut in order to actually have a reasonable chance of cutting whateverthebloodyhairpullinghellitisthatthebloodyhairpullingInvestigator is looking for? In enough numbers to actually believe the images you get have something to do with reality?
Or because once you get cutting you can't stop the ultratome just keeps cutting ahhhhpleasenosomebodyturnitoff!! and geez, there are all these sections, I suppose I have to Look At Them.
What, me, loosing it? Just because classes are on and profs want demos and images of The Things The Class Just Made and we're teaching TEM and reseachers are walking in doing Microscopy! and it's just the beginning of the semester and the chillers acting up and I need parts for the sputter coater and ... I'll go hide under a table now.
Phil
On 09/15/2015 13:39 , AssociationManagement-at-microscopy.org wrote: } } Ask a Microscopist } } } The following Ask a Microscopist form submission was received from your website. } } } Name: Connie Cummings } } School: MSA Member } } Grade/Education Level: Graduate } } Location: Durham, NC US } } Email: ultrapathimaging-at-gmail.com } } Subject: Sampling } } Your Question: An ongoing hair-pulling dispute between me, the microscopist, and almost every person doing research: Why did you take so many photomicrographs for each sample? Wouldn't say two or three photographs be just as good as the 5 to 10 you took. I, the investigation, still can't get over you embedding 6 blocks and sectioning all those blocks as semi-things! } HOW DO YOU HANDLE THIS SCENARIO? HOW DO YOU DEMONSTRATE THAT MORE IN EM IS ACTUALLY BETTER! }
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 32 -- From oshel1pe-at-cmich.edu Tue Sep 15 15:48:32 2015 7, 32 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8FKmWU9015542 7, 32 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 15:48:32 -0500 7, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 7, 32 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8FKmPOM019971; 7, 32 -- Tue, 15 Sep 2015 16:48:30 -0400 7, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 7, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 7, 32 -- (TLS) id 14.3.248.2; Tue, 15 Sep 2015 16:47:54 -0400 7, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 7, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 7, 32 -- 14.3.248.2; Tue, 15 Sep 2015 16:47:53 -0400 7, 32 -- Message-ID: {55F883F9.40502-at-cmich.edu} 7, 32 -- Date: Tue, 15 Sep 2015 16:47:53 -0400 7, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 32 -- MIME-Version: 1.0 7, 32 -- To: micro {microscopy-at-microscopy.com} , {ultrapathimaging-at-gmail.com} 7, 32 -- Subject: Re: Ask a Microscopist 7, 32 -- References: {535726763.1684.1442338747552.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 7, 32 -- In-Reply-To: {535726763.1684.1442338747552.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 7, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 7, 32 -- Content-Transfer-Encoding: 7bit 7, 32 -- X-Originating-IP: [141.209.2.100] 7, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 32 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 7, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 7, 32 -- X-CanItPRO-Stream: default 7, 32 -- X-Canit-Stats-ID: 05PhwMud2 - cc23213da882 - 20150915 7, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Most of the journals have been spoken for, with the exception of Metallurgical Transactions, for which there have been no inquiries, and the ASTM volumes as indicated here: http://www.georgesbasement.com/galootsales/Sale07262015/MetallurgicalJournals.htm
However, during the cleanout of Amenex's storage facility, the following item has become accessible: a Joyce Loebl double-beam microdensitometer, which we obtained in working condition at a cash & carry sale, but for which we never found a use. It is functional and has been kept under cover for the last fifteen years, but the glass platen has gotten broken. I kept the pieces to facilitate measurements for a replacement. We cannot ship it, so the willing recipient will have to find transport. Time is short, as the storage unit is close to getting its final cleanout. The microdensitometer can just about be picked up and carried a few feet by one person (which I've done ... while breaking that glass plate with an errant squeeze) and it will fit in the trunk of an automobile. Packing is problematic for want of space in which to construct & store a crate. If you don't take it, it's going to the recycling center when our trash haulers do that final cleanout. Price: Totally free. It's located at Public Storage on Route 3 in West Chester, Pennsylvania, about equidistant between Philadelphia and Wilmington, Delaware.
==============================Original Headers============================== 5, 29 -- From amenex-at-amenex.com Tue Sep 15 16:27:43 2015 5, 29 -- Received: from ld120.inmotionhosting.com (ld120.inmotionhosting.com [74.124.198.158]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8FLRhC3004926 5, 29 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 16:27:43 -0500 5, 29 -- Received: from localhost ([127.0.0.1]:35561 helo=ld120.inmotionhosting.com) 5, 29 -- by ld120.inmotionhosting.com with esmtpsa (TLSv1:RC4-SHA:128) 5, 29 -- (Exim 4.85) 5, 29 -- (envelope-from {amenex-at-amenex.com} ) 5, 29 -- id 1ZbxlM-00086N-67; Tue, 15 Sep 2015 14:27:41 -0700 5, 29 -- Received: from 72.78.196.173 ([72.78.196.173]) by ld120.inmotionhosting.com 5, 29 -- (Horde Framework) with HTTP; Tue, 15 Sep 2015 21:27:40 +0000 5, 29 -- Date: Tue, 15 Sep 2015 21:27:40 +0000 5, 29 -- Message-ID: {20150915212740.Horde.Rybwaxfr76mw27zKcoH5Sg9-at-ld120.inmotionhosting.com} 5, 29 -- From: amenex-at-amenex.com 5, 29 -- To: microscopy-at-microscopy.com 5, 29 -- Cc: "George Langford, Sc.D." {amenex-at-amenex.com} 5, 29 -- Subject: Update on the metallurgical journals giveaway ... and another 5, 29 -- item's availability 5, 29 -- User-Agent: Internet Messaging Program (IMP) H5 (6.1.4) 5, 29 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Disposition: inline 5, 29 -- X-OutGoing-Spam-Status: No, score=-102.9 5, 29 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 5, 29 -- X-AntiAbuse: Primary Hostname - ld120.inmotionhosting.com 5, 29 -- X-AntiAbuse: Original Domain - microscopy.com 5, 29 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 5, 29 -- X-AntiAbuse: Sender Address Domain - amenex.com 5, 29 -- X-Get-Message-Sender-Via: ld120.inmotionhosting.com: authenticated_id: amenex-at-amenex.com ==============================End of - Headers==============================
Your conversation is so refreshing! Connie loves her preparations and shoots many photos, Tina points at the diverse aspects of each sample and John mentions how easier is to get photos in our digital era.
So where is the problem if flooded with photos? Are they not all of them unique snapshots of each sample? Why is a researcher bothered by them?
Well, the answer is simple but sometimes difficult to say. To appreciate morphology needs affection, brain and time. That makes it less attractive to people inclined to approaches that are easier to interpret, such as immunological and molecular ones.
In Biology, Morphology studies will remain the most straightforward approach to this hidden universe called microcosm. Morphology is a Queen that will always award her devotees! (I just finished a long series of SEM shoots, it is above midnight here and I felt I have to defend my Queen...)
----- Original Message ----- X-from: {tina-at-pbrc.hawaii.edu} To: {eikonika-at-otenet.gr} Sent: Tuesday, September 15, 2015 10:34 PM
Dear Listers,
Realizing that the request may be considered a bit naiive, but could someone please share, or point me to good resource on choosing fluids for diffusion pumps? Maybe even cross-reference table for Fomblin and hydrocarbon oils?
I am trying to return old Electroscan SEM back to life and considering charging Fomblin instead of mineral oil into its Varian M-2 dirrusion pumps...
Thanks beforehand, -- Valery Ray - also with AIM Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
Time is expensive! - the time taken to treat the plants or animals, take samples, process them, etc. Imaging is cheap, and you don't want to have to go back and redo imaging because you didn't have enough samples to begin with. And sectioning doesn't take that long for an experienced person. Nor is it much more effort to run multiple samples.
As an aside, in plant biology, it sometimes takes a year to get transgenics, yet people are often still unwilling to spend more than about a minute taking the key image showing the phenotype/gene expression. Grrr.
Another question is how many replicates are needed in experiments - for statistical differences to be detected? Also, how do you know what are the representative morphologies without multiple samples? Is any quantifiction needed? It often is, these days, you can't get away with saying "this is a representative image" so much any more. And if you don't look closely at your tissues and cells, you might miss something subtle.
Sigh.
cheers, Rosemary
Rosemary White CSIRO Agriculture GPO Box 1600 Canberra, ACT 2601
T 02 6246 5475 E rosemary.white-at-csiro.au
On 16/09/15 5:56 AM, "John.Mardinly-at-asu.edu" {John.Mardinly-at-asu.edu} wrote:
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Email: rrw3q-at-virginia.edu Name: Richard White
Organization: University of virginia
Title-Subject: [Filtered] EDS Controller
Message: We had our TEM Gresham EDS motor controller for our high angle Si/Li detector die on us and I was wondering if anyone has upgraded their system and has one lying around. It is a Gresham model 900-0820 - small white box that just toggles the motor to send the detector In/Out.
Thanks,
Richard
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I recommend that you look up the following report published by Bernie Kestel of Argonne National Lab. the 1994 Microscopy Society of America Outstanding Technologist Award winner.
ANL-80-120/Rev.1 Polishing Methods for Metallic and Ceramic Transmission Electron Microscopy Specimens by B.J. Kestel (1986) DE89016686 NTIS Issue Number 199005
A reference that should be in every microscopist's library of specimen preparation techniques. It is downloadable (Free) from the National Technical Information Service.
It documents 30+ years of electropolishing techniques for TEM specimen prep that Bernie developed before he retired and gives detailed electropolishing solutions and conditions.
Cheers,
Nestor Your Friendly Neighborhood SysOp
PS. The receipe for Aluminum by jet polishing is 20% HClO4 / 80% Ethanol, - 60 C, 90V/10-12 nA
On Tue, Sep 15, 2015 at 1:11 PM, {jkrupp-at-deltacollege.edu} wrote:
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Anyone have advice about jet thinning aluminum alloys?
Thinking of trying it, but need ideas. Anyone doing it or know someone who is?
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
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=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center NanoScience and Technology Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
Chapter 5 of my book "Vacuum Methods in Electron Microscopy" (Wilbur C. Bigelow. Portland Press, 1994) contains a rather extensive discusswion of the characteristics of varipus common diffusion pump fluids, plus a discussion of the factors to consider in chosing a fluid.
Fomblin is great if you are pumping something reactive like oxygen or fluorine. But for a EM you just want something that is low backstreaming. Look at something like Santovac 5, that is what is used in Jeol scopes. It is pretty spendy.
Also look at adding a alumina-media fore line trap, this will help stop mechanical pump oil from back streaming into the scope during roughing and contaminating the santovac.
I did the expensive route and replaced the diff pump with a Varian turbo backed with a Edwards oilless scroll pump. I think those two pumps are worth more than my SEM.
-Jerry
} On Sep 15, 2015, at 4:57 PM, vray-at-partbeamsystech.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } Realizing that the request may be considered a bit naiive, but could } someone please share, or point me to good resource on choosing fluids } for diffusion pumps? Maybe even cross-reference table for Fomblin and } hydrocarbon oils? } } I am trying to return old Electroscan SEM back to life and considering } charging Fomblin instead of mineral oil into its Varian M-2 dirrusion } pumps... } } Thanks beforehand, } -- } Valery Ray - also with AIM Lab, UMDCP } ======================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844, USA } Phone: +1-978-296-5063 } E-Mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } UMD E-Mail: vray-at-umd.edu } } ==============================Original Headers============================== } 4, 32 -- From vray-at-partbeamsystech.com Tue Sep 15 18:44:15 2015 } 4, 32 -- Received: from nm13-vm0.bullet.mail.bf1.yahoo.com (nm13-vm0.bullet.mail.bf1.yahoo.com [98.139.213.79]) } 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8FNiFsx015735 } 4, 32 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 18:44:15 -0500 } 4, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1442360654; bh=qno3G1jP/zkrloH6Wrvt7pvgIxphptEHfKzzZbMRWHw=; h=To:From:Subject:Date:From:Subject; b=AGin1gdOS1llgZvEi8dJJfE2yn77VBVlLXHalh6D1xMEln/nugGmvFSq9TgWCXeLuMXa/az+Zycgyd2lIqRfE0RE9+YCFlDBJvmp3Lv1Aehz/UmW1N44nNpGkwEwWuelLmBotmjbgM/2H+fwdhPDnb9MMuDV8UkpDluugBBQ8N3cEP8D1xgNbR9DrAYQFyukM/wftuMriaH98y4IgV3+bw34DuPkKtaMvzq2QkXs6jzNksO1xp7C7pxMsR3y7TYZY9QeIq58bOOvtAhlSDbamT73nxVU9L7sX2zIDa/RXMh/ju6cSguyKPLYfbJtPJQDzDxeOL089nWb+KKKIZUeOw== } 4, 32 -- Received: from [98.139.170.182] by nm13.bullet.mail.bf1.yahoo.com with NNFMP; 15 Sep 2015 23:44:14 -0000 } 4, 32 -- Received: from [98.139.211.160] by tm25.bullet.mail.bf1.yahoo.com with NNFMP; 15 Sep 2015 23:44:14 -0000 } 4, 32 -- Received: from [127.0.0.1] by smtp217.mail.bf1.yahoo.com with NNFMP; 15 Sep 2015 23:44:14 -0000 } 4, 32 -- X-Yahoo-Newman-Id: 291873.22690.bm-at-smtp217.mail.bf1.yahoo.com } 4, 32 -- X-Yahoo-Newman-Property: ymail-3 } 4, 32 -- X-YMail-OSG: w_a.5QcVM1lLkLSeF1QolkjzAc4g6raI84Zl_ZeZH2fMiDg } 4, 32 -- .TkJTqrfgPV80Vy0IMTSKc5_FHonzFL10GS7hJEe4247LziygPCXlxoWx0G. } 4, 32 -- YdUEtN.pLOPD_akjVJXrsG6iVS3toKnHQlCoSHsTMPFPybmrsH7D.l0dnr_k } 4, 32 -- mTIvDeBNxl32A0rtavoCY6PimyF7n0oDunuDA4nFdGPRKOqgZKntqq2Ka912 } 4, 32 -- dHGPq9Pb714Dtny5yLF.4a.I0H5W5Exa3PcGadHFCJhUUiGOMw3G6fqrj3C4 } 4, 32 -- NwfKyMDFOEX2MJDJzGHPEbsPFoAWOa5G70yh84yUBEglTZgpRw.UDyUnvUoC } 4, 32 -- _2tE.AP6hffjtO0yxpbdSwtkkL8F8eDa32XkY0u5P9mFua.O5Djv.hPz1CTl } 4, 32 -- EBQIvUC..9lJ19.qTvBPTfmnZiULFmZ1XGMJi0qWbBHA4.QgMJcMRoS1Dmgw } 4, 32 -- .EALwMNqQoiEH7bt.P5vWdGYB4KXHzftpHjSq1EQPyJ2RNCKzE4PfJ4Id.OX } 4, 32 -- 0kX43.FavC2pNDwqzSaRLT8WJuhGJRVEDFy3qYn1qtmmTjCsH_ZQoGP07WJX } 4, 32 -- S8Q-- } 4, 32 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- } 4, 32 -- To: microscopy-at-microscopy.com } 4, 32 -- From: Valery Ray {vray-at-partbeamsystech.com} } 4, 32 -- Subject: Converting diffusion pumps to Fomblin } 4, 32 -- Message-ID: {55F8AD4D.9050306-at-partbeamsystech.com} } 4, 32 -- Date: Tue, 15 Sep 2015 19:44:13 -0400 } 4, 32 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 } 4, 32 -- Thunderbird/38.2.0 } 4, 32 -- MIME-Version: 1.0 } 4, 32 -- Content-Type: text/plain; charset=utf-8; format=flowed } 4, 32 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 35 -- From jerry.biehler-at-gmail.com Tue Sep 15 21:53:11 2015 6, 35 -- Received: from mail-ig0-f173.google.com (mail-ig0-f173.google.com [209.85.213.173]) 6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8G2rBPw023703 6, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 21:53:11 -0500 6, 35 -- Received: by igcpb10 with SMTP id pb10so26919702igc.1 6, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 19:53:10 -0700 (PDT) 6, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 35 -- d=gmail.com; s=20120113; 6, 35 -- h=content-type:mime-version:subject:from:in-reply-to:date 6, 35 -- :content-transfer-encoding:message-id:references:to; 6, 35 -- bh=6LatNNcioEzsM3NBUumVTtS5h3gT5SQCkx/5/zkuZEE=; 6, 35 -- b=hSn+akzLeL7UdqXvTy1tdKX8NIsudn6UaR4mz6o89f0fU2fPiMYGUMAnbof3VyBOOF 6, 35 -- CheLigKdnYCZdodVE+Bv5UgtpaMX/I2d5RFkIi8TU8zXJUNNH3Ny7Rh46+Qc9rj9ST1e 6, 35 -- ZWfA73yDhPP3C0IUnbV9W46OH1td7qKmdVWFLDzW53AqD9F48p3sjKphR0fsNQUi//47 6, 35 -- EsxQnTyGBTTaoR0TOQABePpjuhO6PyPQZ/sOD2jeUvrqnTxDR18ThjREJmwP9u3rKzcb 6, 35 -- w+u9zinofNBifpm5zzzv02yZPHqVfUwUw0GPoyQIz6R5XCYqyQx6cIyfj/ieKzerzhX6 6, 35 -- VZnQ== 6, 35 -- X-Received: by 10.50.128.165 with SMTP id np5mr5488904igb.72.1442371989749; 6, 35 -- Tue, 15 Sep 2015 19:53:09 -0700 (PDT) 6, 35 -- Received: from [192.168.1.2] ([104.169.195.240]) 6, 35 -- by smtp.googlemail.com with ESMTPSA id x1sm1017687igl.14.2015.09.15.19.53.08 6, 35 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 6, 35 -- Tue, 15 Sep 2015 19:53:08 -0700 (PDT) 6, 35 -- Content-Type: text/plain; charset=us-ascii 6, 35 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 6, 35 -- Subject: Re: [Microscopy] Converting diffusion pumps to Fomblin 6, 35 -- From: Jerry Biehler {jerry.biehler-at-gmail.com} 6, 35 -- In-Reply-To: {201509152357.t8FNvluQ001988-at-ns.microscopy.com} 6, 35 -- Date: Tue, 15 Sep 2015 19:53:06 -0700 6, 35 -- Message-Id: {A0CD64DE-F4B6-41E9-8EA3-46589E151B01-at-gmail.com} 6, 35 -- References: {201509152357.t8FNvluQ001988-at-ns.microscopy.com} 6, 35 -- To: vray-at-partbeamsystech.com, Microscopy-at-microscopy.com 6, 35 -- X-Mailer: Apple Mail (2.2104) 6, 35 -- Content-Transfer-Encoding: 8bit 6, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8G2rBPw023703 ==============================End of - Headers==============================
Yorgos, it will depend on tissue and parameters of ..you know...(treatment prior to as well as fixation, processing for SEM, observation in SEM and then parameters of reprocessing specs for TEM.) It can be done and can be a valuable supplementing info to the images documented by SEM. (will send you an old EMSA-MSA-abstract [1990, with images] with an example of "arterial" SEM to TEM by separate-personal - mail),
best wishes and regards, Wolfgang
Wolfgang MUSS PhD Member of MSA SALZBURG, AUSTRA =====================================================================
Von: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr] Gesendet: Mittwoch, 16. September 2015 09:11 An: Muß Wolfgang Betreff: [Microscopy] TEM after SEM on the same sample?
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Hello Has anybody done TEM after SEM on the same sample (talking about soft animal tissue) with good or acceptable morphology? Thanks -yorgos
I would explain that: 1) Every cell is unique, therefore a sample size of one is not scientifically relevant. (statistics). 2) EM sample preparation is somewhat time intensive, and it would be a poor value to not see more of the sample after that effort.
Good luck, ~Gregg
Gregg Sobocinski Microscope Imaging Specialist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
On Tue, Sep 15, 2015 at 3:02 PM, {oshel1pe-at-cmich.edu} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *************************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } **************************************************************************************** } } } } Ask a Microscopist } } } } } } The following Ask a Microscopist form submission was received from your website. } } } } } } Name: Connie Cummings } } } } School: MSA Member } } } } Grade/Education Level: Graduate } } } } Location: Durham, NC US } } } } Email: ultrapathimaging-at-gmail.com } } } } Subject: Sampling } } } } Your Question: An ongoing hair-pulling dispute between me, the microscopist, and almost every person doing research: Why did you take so many photomicrographs for each sample? Wouldn't say two or three photographs be just as good as the 5 to 10 you took. I, the investigation, still can't get over you embedding 6 blocks and sectioning all those blocks as semi-things! } } HOW DO YOU HANDLE THIS SCENARIO? HOW DO YOU DEMONSTRATE THAT MORE IN EM IS ACTUALLY BETTER! } } } } } } ==============================Original Headers============================== } 5, 32 -- From oshel1pe-at-cmich.edu Tue Sep 15 13:55:51 2015 } 5, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) } 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8FItoHZ016191 } 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 13:55:50 -0500 } 5, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) } 5, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8FItm5T018670 } 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 15 Sep 2015 14:55:49 -0400 } 5, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by } 5, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server } 5, 32 -- (TLS) id 14.3.248.2; Tue, 15 Sep 2015 14:55:48 -0400 } 5, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by } 5, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id } 5, 32 -- 14.3.248.2; Tue, 15 Sep 2015 14:55:48 -0400 } 5, 32 -- Message-ID: {55F869B4.7010204-at-cmich.edu} } 5, 32 -- Date: Tue, 15 Sep 2015 14:55:48 -0400 } 5, 32 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} } 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} } 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 5, 32 -- MIME-Version: 1.0 } 5, 32 -- To: micro {microscopy-at-microscopy.com} } 5, 32 -- Subject: Re: Ask a Microscopist } 5, 32 -- References: {535726763.1684.1442338747552.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} } 5, 32 -- In-Reply-To: {535726763.1684.1442338747552.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} } 5, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed } 5, 32 -- Content-Transfer-Encoding: 7bit } 5, 32 -- X-Originating-IP: [141.209.2.100] } 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) } 5, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) } 5, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 } 5, 32 -- X-CanItPRO-Stream: default } 5, 32 -- X-Canit-Stats-ID: 02PhuTNja - 8c77604ccdfa - 20150915 } 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 } ==============================End of - Headers==============================
Yes. EDS, even. I just had the sections (thin sections) mounted on the TEM grid ready for the TEM, then put them in the SEM. This works best if the SEM stub has hole drilled in it just less than 3 mm diameter, so that there is a void space below the grid with samples. Be careful though. The lower kVs used in SEM will result in greater beam-stopping by the sample, therefore more energy deposited in the sample and a greater likelihood of rupturing the section or any supporting film.
Phil
} Hello } Has anybody done TEM after SEM on the same sample (talking about soft animal } tissue) with good or acceptable morphology? } Thanks -yorgos } } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.net www.aim.cat } ************************************
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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Yorgos, it will depend on tissue and parameters of ..you know...(treatment prior to as well as fixation, processing for SEM, observation in SEM and then parameters of reprocessing specs for TEM.) It can be done and can be a valuable supplementing info to the images documented by SEM. (will send you an old EMSA-MSA-abstract [1990, with images] with an example of "arterial" SEM to TEM by separate-personal - mail),
best wishes and regards, Wolfgang
Wolfgang MUSS PhD Member of MSA SALZBURG, AUSTRA =====================================================================
Von: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr] Gesendet: Mittwoch, 16. September 2015 09:11 An: Muß Wolfgang Betreff: [Microscopy] TEM after SEM on the same sample?
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Hello Has anybody done TEM after SEM on the same sample (talking about soft animal tissue) with good or acceptable morphology? Thanks -yorgos
Hi, Does anyone have experience with the kevex omicron xrf I am in need of some information with respect to analyzing the readings.
Alex Besenyo PhD
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Title-Subject: [Filtered] Investigator's Questions about Sampling, Sectioning, Photomicrographs
Message: Hi, If you read this yesterday, then I submitted my question in the right place so you can ignore this post. If you haven't read this, please read on and give an old frustrated EM lady some feedback! In an ongoing hair-pulling dispute between me, the microscopist, and almost every person doing research I get these questions: Why did you take so many photomicrographs for each sample? Wouldn't you say two or three photographs would be just as good as the 5 to 10 you took? I, the investigator, am still trying to get over you embedding 6 blocks and sectioning all those blocks as semi-thins! HOW DO YOU HANDLE THIS SCENARIO? HOW DO YOU DEMONSTRATE THAT MORE IN EM IS ACTUALLY BETTER!
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I should add that I've also done SEM-then-TEM on tissue samples prepared for TEM, en bloc stained or not, then dried and sputter coated for SEM. After the SEM, "rehydrate" in 100% ethanol, embed and section for TEM. The morphology in the thin sections likely won't be as good as tissue processed for TEM, embedded, sectioned, stained (or not), but depending on the study, it can still be useful. Imaging something like (vertebrate liver) bile canaliculi first in the SEM, then in sections in the TEM is a good example of this. Note: the sputter-coated layer of metal causes no issues when sectioning.
Phil
Yorgos,
Yes. EDS, even. I just had the sections (thin sections) mounted on the TEM grid ready for the TEM, then put them in the SEM. This works best if the SEM stub has hole drilled in it just less than 3 mm diameter, so that there is a void space below the grid with samples. Be careful though. The lower kVs used in SEM will result in greater beam-stopping by the sample, therefore more energy deposited in the sample and a greater likelihood of rupturing the section or any supporting film.
Phil
} Hello } Has anybody done TEM after SEM on the same sample (talking about soft animal } tissue) with good or acceptable morphology? } Thanks -yorgos } } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.net www.aim.cat } ************************************
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 32 -- From oshel1pe-at-cmich.edu Wed Sep 16 09:14:37 2015 7, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8GEEZfC008451 7, 32 -- for {microscopy-at-microscopy.com} ; Wed, 16 Sep 2015 09:14:35 -0500 7, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 7, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8GEDdcq006189 7, 32 -- for {microscopy-at-microscopy.com} ; Wed, 16 Sep 2015 10:13:52 -0400 7, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 7, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 7, 32 -- (TLS) id 14.3.248.2; Wed, 16 Sep 2015 10:13:49 -0400 7, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 7, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 7, 32 -- 14.3.248.2; Wed, 16 Sep 2015 10:13:49 -0400 7, 32 -- Message-ID: {55F9791D.5030603-at-cmich.edu} 7, 32 -- Date: Wed, 16 Sep 2015 10:13:49 -0400 7, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 32 -- MIME-Version: 1.0 7, 32 -- To: micro {microscopy-at-microscopy.com} 7, 32 -- Subject: Re: [Microscopy] TEM after SEM on the same sample? 7, 32 -- References: {201509160727.t8G7Raxi004466-at-ns.microscopy.com} 7, 32 -- In-Reply-To: {201509160727.t8G7Raxi004466-at-ns.microscopy.com} 7, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 7, 32 -- Content-Transfer-Encoding: 7bit 7, 32 -- X-Originating-IP: [141.209.2.100] 7, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 32 -- X-Spam-Score: -1.20 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,_L_SUPPORT,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 7, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 7, 32 -- X-CanItPRO-Stream: default 7, 32 -- X-Canit-Stats-ID: 02PhOdQmM - e9160a49b9e9 - 20150916 7, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Dear All, Can someone lead me to a good set of protocols for fixing chitin. My biology friends tell me that conventional methods for fixing chitin often fail to adhere the plastic to the fibers. I am doing an analytical study of the inorganic components in the chitin so I donât want to use any staining and I want to preserve the integrity of the Ca phases present. Thanks from a materials oriented microscopist! Ken
Kenneth JT Livi, PhD Director, Materials Characterization and Processing Center and HRAEM Materials Science and Engineering 3400 N Charles Street Johns Hopkins University Baltimore, Maryland 21218 USA klivi-at-jhu.edu
==============================Original Headers============================== 4, 33 -- From prvs=694bdd14a=klivi-at-jhu.edu Wed Sep 16 09:47:09 2015 4, 33 -- Received: from IPMTW2.johnshopkins.edu (ipmtw2.johnshopkins.edu [128.220.229.150]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8GEl8Vm030509 4, 33 -- for {microscopy-at-microscopy.com} ; Wed, 16 Sep 2015 09:47:08 -0500 4, 33 -- X-IronPort-AV: E=Sophos;i="5.17,539,1437451200"; 4, 33 -- d="scan'208";a="90761909" 4, 33 -- Received: from esgebex4.win.ad.jhu.edu ([10.15.89.9]) 4, 33 -- by IPMTW2.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 16 Sep 2015 10:47:03 -0400 4, 33 -- Received: from ESGEBEX1.win.ad.jhu.edu (10.15.89.6) by ESGEBEX4.win.ad.jhu.edu 4, 33 -- (10.15.89.9) with Microsoft SMTP Server (TLS) id 15.0.1076.9; Wed, 16 Sep 4, 33 -- 2015 10:47:03 -0400 4, 33 -- Received: from ESGEBEX1.win.ad.jhu.edu ([fe80::2171:57e9:1713:fc1b]) by 4, 33 -- ESGEBEX1.win.ad.jhu.edu ([fe80::2171:57e9:1713:fc1b%31]) with mapi id 4, 33 -- 15.00.1076.000; Wed, 16 Sep 2015 10:47:02 -0400 4, 33 -- From: KEN Livi {klivi-at-jhu.edu} 4, 33 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 33 -- CC: KEN Livi {klivi-at-jhu.edu} 4, 33 -- Subject: protocol for fixing chitin 4, 33 -- Thread-Topic: protocol for fixing chitin 4, 33 -- Thread-Index: AQHQ8I6Ko+9qZDDLZEm5+gTAYjOM6A== 4, 33 -- Date: Wed, 16 Sep 2015 14:46:59 +0000 4, 33 -- Message-ID: {0BFCF35F-2761-4CDD-AFD5-0988909E76DB-at-jhu.edu} 4, 33 -- Accept-Language: en-US 4, 33 -- Content-Language: en-US 4, 33 -- X-MS-Has-Attach: 4, 33 -- X-MS-TNEF-Correlator: 4, 33 -- x-ms-exchange-transport-fromentityheader: Hosted 4, 33 -- x-originating-ip: [10.181.192.120] 4, 33 -- Content-Type: text/plain; charset="utf-8" 4, 33 -- Content-ID: {4C800210512D4D4A91245C9C44C628D9-at-exchange.johnshopkins.edu} 4, 33 -- MIME-Version: 1.0 4, 33 -- Content-Transfer-Encoding: 8bit 4, 33 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t8GEl8Vm030509 ==============================End of - Headers==============================
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Hi, I work on cell walls of plants not fungi. But I believe the issue is related. From my reading of the literature (and this is far from complete), classical chemical crosslinking fixatives, like formaldhyde and glutaraldehyde, react to only a limited extent with cell wall polymers. I have always used fixatives when I want to keep the tissue or cytoplasm in good shape. But the wall itself I think does not need fixation (except perhaps to inactivate cell wall degrading enzymes that live out there). Cell walls are tough and can withstand typical dehydration / embedding schedules without too much trouble. You wrote about plastic adhering to the chitin. This seems like more of an infiltrating/embedding issue. An old trick that works to embed certain tricky plant samples is to start infiltrating with really low concentrations of your plastic, like 1% then 2%, 5%, 10% and then as normal.
Hope this helps, Tobias Baskin
On 9/16/15 10:49 AM, klivi-at-jhu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } Can someone lead me to a good set of protocols for fixing chitin. My biology friends tell me that conventional methods for fixing chitin often fail to adhere the plastic to the fibers. I am doing an analytical study of the inorganic components in the chitin so I donât want to use any staining and I want to preserve the integrity of the Ca phases present. } Thanks from a materials oriented microscopist! } Ken } } Kenneth JT Livi, PhD } Director, Materials Characterization and Processing Center and HRAEM } Materials Science and Engineering } 3400 N Charles Street } Johns Hopkins University } Baltimore, Maryland 21218 USA } klivi-at-jhu.edu } }
-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ University of Mass. / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ Amherst, Massachusetts / /___ / \ \___/ \_____ USA 413-545-1533 www.bio.umass.edu/biology/baskin
Any chitin in particular? Arthropod cuticle, fungus, or somebody else? This is a ubiquitous polysaccharide, but not necessarily identical. Especially for minerals, like Ca. And: Your question implies you're wanting to do TEM, but perhaps SEM would be better? If you're not interested in the ultrastructure of biological components (like cells), you could let the critter dry, then cryofracture in liquid nitrogen. This would expose the internal structure of the chitin and contained mineralized phases. If you need a polished surface for x-ray spectroscopy or EBSD, then this wouldn't work.
Phil
} Dear All, } Can someone lead me to a good set of protocols for fixing chitin. My biology friends tell me that conventional methods for fixing chitin often fail to adhere the plastic to the fibers. I am doing an analytical study of the inorganic components in the chitin so I donât want to use any staining and I want to preserve the integrity of the Ca phases present. } Thanks from a materials oriented microscopist! } Ken } } Kenneth JT Livi, PhD } Director, Materials Characterization and Processing Center and HRAEM } Materials Science and Engineering } 3400 N Charles Street } Johns Hopkins University } Baltimore, Maryland 21218 USA } klivi-at-jhu.edu -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 32 -- From oshel1pe-at-cmich.edu Wed Sep 16 13:05:49 2015 4, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8GI5nP7009715 4, 32 -- for {microscopy-at-microscopy.com} ; Wed, 16 Sep 2015 13:05:49 -0500 4, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 4, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8GI5jAf024581; 4, 32 -- Wed, 16 Sep 2015 14:05:46 -0400 4, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 4, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 4, 32 -- (TLS) id 14.3.248.2; Wed, 16 Sep 2015 14:05:45 -0400 4, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 4, 32 -- 14.3.248.2; Wed, 16 Sep 2015 14:05:44 -0400 4, 32 -- Message-ID: {55F9AF78.1080305-at-cmich.edu} 4, 32 -- Date: Wed, 16 Sep 2015 14:05:44 -0400 4, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 32 -- MIME-Version: 1.0 4, 32 -- To: {klivi-at-jhu.edu} , micro {microscopy-at-microscopy.com} 4, 32 -- Subject: Re: [Microscopy] protocol for fixing chitin 4, 32 -- References: {201509161535.t8GFZEBS013412-at-ns.microscopy.com} 4, 32 -- In-Reply-To: {201509161535.t8GFZEBS013412-at-ns.microscopy.com} 4, 32 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 4, 32 -- Content-Transfer-Encoding: 8bit 4, 32 -- X-Originating-IP: [141.209.2.100] 4, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 4, 32 -- X-CanItPRO-Stream: default 4, 32 -- X-Canit-Stats-ID: 02PhS5KAF - 2da327ce74b6 - 20150916 4, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
On Sep 16, 2015, at 8:20 AM, klivi-at-jhu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } Can someone lead me to a good set of protocols for fixing chitin. My biology friends tell me that conventional methods for fixing chitin often fail to adhere the plastic to the fibers. I am doing an analytical study of the inorganic components in the chitin so I dont want to use any staining and I want to preserve the integrity of the Ca phases present. } Thanks from a materials oriented microscopist!
I have never tried this, but I remember way back that some of my materials science friends said they needed a secret ingredient to make the embedding plastic stick to their samples.
I think I remember it as something called Z-1, but a search didn't find anything. Looked at EMS and they have Z-6040 which looks like it's supposed to do the same thing.
Might be worth a try.
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
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It's a new school year and I have alerted my EM students that they should follow the Listserver to learn more about microscopy and, if the can get up the courage, to use it for questions they can't otherwise figure out.
Please be kind to these students, some don't have any experience with listservers and may be a little rough around the edges. They are mostly good students trying to become better scientists.
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
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I did a lot of this in the 1980's on human ovary. We were looking for particular epithelia cell populations so we would find the cells in the SEM, dissect out the area and then do TEM on that area.
As a control we also worked backwards. If we saw the cell population of interest in TEM sections, or semi-thin sections, we would then dissolve out the embedding resin and look at the block in the SEM.
Worked quite well and the work was published. It was related to damage done to the epithelia layer of the ovary during surgical procedures. The ultrastructure in the TEM after SEM preparation wasn't super good but it gave the information that was required.
This was all pre-PDF and pre-computers so give me a few days and I will dig out my old notes, scan them and send them to you.
Have a great day
Allan
On 16/09/2015, at 7:17 PM, {eikonika-at-otenet.gr} {eikonika-at-otenet.gr} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello } Has anybody done TEM after SEM on the same sample (talking about soft animal } tissue) with good or acceptable morphology? } Thanks -yorgos } } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.net www.aim.cat } ************************************
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254 EM Centre: http://ocem.otago.ac.nz/
==============================Original Headers============================== 17, 23 -- From allan.mitchell-at-stonebow.otago.ac.nz Wed Sep 16 15:32:59 2015 17, 23 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 17, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8GKWw6i010099 17, 23 -- for {Microscopy-at-Microscopy.Com} ; Wed, 16 Sep 2015 15:32:59 -0500 17, 23 -- Received: from anatomy.otago.ac.nz (anatomyy.otago.ac.nz [139.80.40.227]) 17, 23 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id t8GKWrtH007138; 17, 23 -- Thu, 17 Sep 2015 08:32:53 +1200 17, 23 -- Received: from ou040139.otago.ac.nz (ou040139.otago.ac.nz [139.80.40.139]) 17, 23 -- by anatomy.otago.ac.nz (Postfix) with ESMTP id 9138CD046BA4; 17, 23 -- Thu, 17 Sep 2015 08:32:53 +1200 (NZST) 17, 23 -- Subject: Re: [Microscopy] TEM after SEM on the same sample? 17, 23 -- Mime-Version: 1.0 (Apple Message framework v1084) 17, 23 -- Content-Type: text/plain; charset=us-ascii 17, 23 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 17, 23 -- In-Reply-To: {201509160717.t8G7HaqG029247-at-ns.microscopy.com} 17, 23 -- Date: Thu, 17 Sep 2015 08:32:53 +1200 17, 23 -- Cc: Microscopy-at-Microscopy.Com 17, 23 -- Message-Id: {7BDEA4BE-EF69-4228-823D-B81510846C26-at-stonebow.otago.ac.nz} 17, 23 -- References: {201509160717.t8G7HaqG029247-at-ns.microscopy.com} 17, 23 -- To: {eikonika-at-otenet.gr} 17, 23 -- X-Mailer: Apple Mail (2.1084) 17, 23 -- Content-Transfer-Encoding: 8bit 17, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8GKWw6i010099 ==============================End of - Headers==============================
From mike.sfsd4f564s6df45dsriam-at-gmail.com Wed Sep 16 16:44:08 2015 Return-Path: {mike.sfsd4f564s6df45dsriam-at-gmail.com} Received: from gmail.com ([61.79.228.179]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t8GLi5Gd001420 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 16 Sep 2015 16:44:07 -0500 Message-ID: {941AB288.2200DEFE-at-gmail.com}
} On Sep 16, 2015, at 11:48 AM, jkrupp-at-deltacollege.edu wrote: } } } Dear All, } } Can someone lead me to a good set of protocols for fixing chitin. My biology friends tell me that conventional methods for fixing chitin often fail to adhere the plastic to the fibers. I am doing an analytical study of the inorganic components in the chitin so I donÂt want to use any staining and I want to preserve the integrity of the Ca phases present. } } Thanks from a materials oriented microscopist! } } I have never tried this, but I remember way back that some of my materials science friends said they needed a secret ingredient to make the embedding plastic stick to their samples. } } I think I remember it as something called Z-1, but a search didn't find anything. Looked at EMS and they have Z-6040 which looks like it's supposed to do the same thing. } } Might be worth a try. } } Jon
Dear Jon & Ken, I think that (at least some kinds of) chitin have a waxy coating. If that is true of your chitin, Ken, and if the wax structure is part of what youâre interested inâit may contain some Caâany additive that could dissolve the wax will be problematic. Perhaps Philâs suggestion of SEM to characterize the wax (if any), then prep for TEM would be best. Can chitin be placed in the SEM without treatment and/or coating? If so, then Iâd definitely try it. Yours, Bill
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Waxy epicuticles are common in insects and terrestrial arthropods, but that's about it. Not part of the chitin per se. And any wax has an unfortunate tendency to melt under the beam. Cryomethods are needed to study it - and the wax on plant leaves. That said, waxy coats on critters and plants are very poorly studied and I'm sure have more uses than just slowing or stopping dessication.
Phil
} } On Sep 16, 2015, at 11:48 AM, jkrupp-at-deltacollege.edu wrote: } } } } } Dear All, } } } Can someone lead me to a good set of protocols for fixing chitin. My biology friends tell me that conventional methods for fixing chitin often fail to adhere the plastic to the fibers. I am doing an analytical study of the inorganic components in the chitin so I donÂt want to use any staining and I want to preserve the integrity of the Ca phases present. } } } Thanks from a materials oriented microscopist! } } } } I have never tried this, but I remember way back that some of my materials science friends said they needed a secret ingredient to make the embedding plastic stick to their samples. } } } } I think I remember it as something called Z-1, but a search didn't find anything. Looked at EMS and they have Z-6040 which looks like it's supposed to do the same thing. } } } } Might be worth a try. } } } } Jon } } Dear Jon& Ken, } I think that (at least some kinds of) chitin have a waxy coating. If that is true of your chitin, Ken, and if the wax structure is part of what youâre interested inâit may contain some Caâany additive that could dissolve the wax will be problematic. Perhaps Philâs suggestion of SEM to characterize the wax (if any), then prep for TEM would be best. Can chitin be placed in the SEM without treatment and/or coating? If so, then Iâd definitely try it. } Yours, -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 35 -- From oshel1pe-at-cmich.edu Thu Sep 17 07:21:35 2015 4, 35 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8HCLZir008651 4, 35 -- for {microscopy-at-microscopy.com} ; Thu, 17 Sep 2015 07:21:35 -0500 4, 35 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 4, 35 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8HCLW7Q022668; 4, 35 -- Thu, 17 Sep 2015 08:21:33 -0400 4, 35 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 4, 35 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 4, 35 -- (TLS) id 14.3.248.2; Thu, 17 Sep 2015 08:21:31 -0400 4, 35 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 4, 35 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 4, 35 -- (TLS) id 14.3.248.2; Thu, 17 Sep 2015 08:21:31 -0400 4, 35 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 35 -- cas2.central.cmich.local (141.209.15.141) with Microsoft SMTP Server (TLS) id 4, 35 -- 14.3.248.2; Thu, 17 Sep 2015 08:21:30 -0400 4, 35 -- Message-ID: {55FAB04A.5000307-at-cmich.edu} 4, 35 -- Date: Thu, 17 Sep 2015 08:21:30 -0400 4, 35 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 35 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 35 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 35 -- MIME-Version: 1.0 4, 35 -- To: {wtivol-at-sbcglobal.net} , micro {microscopy-at-microscopy.com} 4, 35 -- Subject: Re: [Microscopy] Re: protocol for fixing chitin 4, 35 -- References: {201509170006.t8H06Tcp021314-at-ns.microscopy.com} 4, 35 -- In-Reply-To: {201509170006.t8H06Tcp021314-at-ns.microscopy.com} 4, 35 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-Originating-IP: [141.209.2.100] 4, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 35 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 4, 35 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 4, 35 -- X-CanItPRO-Stream: default 4, 35 -- X-Canit-Stats-ID: 05PiclxKF - 2f05bd81dc89 - 20150917 4, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Hi, What would anyone out there recommend for a TEM grid carbon coater for ultraclean carbon deposition (dry/UHV system), with precision in the 1-5 nm thickness range and a real-time thickness monitor?
Hi Larry, for carbon coating, you may prefer to use a turbo-pumped system and a fast evaporator. you may have a try on two systems which I have experience with: - Cressington turbo coater 208 (carbon rod and e-beam guns are available) - Leica ACE series (carbon rod and carbon wire are available, to my knowledge; e-beam?) other machines from other companies will do the job as well, I assume.
quartz thickness monitor YES; but real-time thickness: you will have to wait for a few (5 to 10) seconds until the system settles (physics of heat transfer). Upon Carbon evaporation the quartz is getting heated as well and will give you some numbers which are not realistic. At the end, we find this reproducible. Both machines work fine, in our hands, for light shadowing of bio-samples for STEM, TEM or SEM. try to find a lab where you can have a test experiment with your samples, or convince the sales rep to give you a system for a week for several tries. As you do not explicitly state what you are going to do: "TEM grid carbon coater": if you want to produce your own carbon supporting film, you may have first to shadow carbon onto mica, then float this off (on a water surface, e.g. ), and then pick up the C-film with (hydrophilized, glow-discharged) grids. Standard IMO. kind regards - Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: - 16th Europ Microsc Congress EMC http://www.emc2016.fr/en/ 28 Aug - 2 Sept 2016 in Lyon, FR - Microscopy Conference 2017 Dreiländertagung Lausanne, CH 20-25 August 2017 - next Microbiol. conferences: VAAM - Annual Conf March 13-16, 2016, Jena
==============================Original Headers============================== 5, 22 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Fri Sep 18 01:48:01 2015 5, 22 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8I6m0uL025246 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 01:48:00 -0500 5, 22 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 5, 22 -- by localhost (Postfix) with SMTP id DF50660ACD 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 08:47:57 +0200 (CEST) 5, 22 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 5, 22 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id BC9D260A54 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 08:47:57 +0200 (CEST) 5, 22 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 5, 22 -- with Novell_GroupWise; Fri, 18 Sep 2015 08:47:57 +0200 5, 22 -- Message-Id: {55FBCFBB020000540005780E-at-gwsmtp1.uni-regensburg.de} 5, 22 -- X-Mailer: Novell GroupWise Internet Agent 14.2.0 Beta 5, 22 -- Date: Fri, 18 Sep 2015 08:47:55 +0200 5, 22 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 5, 22 -- To: {microscopy-at-microscopy.com} 5, 22 -- Subject: C-Coater 5, 22 -- Mime-Version: 1.0 5, 22 -- Content-Type: text/plain; charset=UTF-8 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- Content-Disposition: inline ==============================End of - Headers==============================
Could others please share their experiences with the premade aqueous OsO4 solutions?
I have been making my own 4% solution, storing it in the fridge, and diluting a small volume just before use.
However our EM lab isn't very busy (3-4 preps every few months, although I am working on trying to get more users!) and I end up having to throw out a lot of the 20ml solution that I make; membranes start looking kind of crappy and with the older solution even if the solution still looks relatively clear. I also see a lot of black specks in my unstained sections.
I don't know of anyone who uses the premade ampoules, so I don't know if there are any significant differences in image quality. They come in such small volumes, which would be really convenient for me!
Thanks in advance!
-Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
I'm writing to this group on a referral basis. I would greatly appreciate a reply if you know of anyone in your professional network that would be a fit for the expertise we are trying to find. The Pacific Northwest National Laboratory is seeking a Post Doc focusing on Scanning Transmission Electron Microscopy. This postdoctoral position is best suited for a person who has recently obtained their Ph.D. on materials characterization primarily using aberration-corrected scanning transmission electron microscopy. The successful candidate will join a team that uses FIB, aberration corrected TEM/STEM, and other characterization methods to understand complex interfaces, and their effect on the material's function. Some interfaces of interest are metal/oxide and metal/semiconductor, and vary from crystalline to amorphous.
Requirements:
- Ph.D. in physics, materials science, engineering, mathematics, chemistry, or related field. - Must have experience operating Cs-corrected STEMs and performing careful analysis of data. - Experience preparing specimens using the focused ion beam (FIB) preferred. - Experience and an interest in image simulations and image analysis preferred. Experience with - MATLAB, C++, or other environments for algorithm development is highly desirable. - Ability to obtain a security clearance, which requires U.S. Citizenship
For the reasons you mention we have been making use of the premade aqueous OsO4 solutions for years. It works well for our own use and it is convenient when another user comes by asking for a few ml's of OsO4. Occasionally we still make up a solution for odd ball recipes or large quantities.
Thanks, Louie
----- Original Message ----- X-from: bicarbaj-at-mtholyoke.edu To: lkerr-at-mbl.edu Sent: Friday, September 18, 2015 11:01:37 AM
Hello everyone,
Could others please share their experiences with the premade aqueous OsO4 solutions?
I have been making my own 4% solution, storing it in the fridge, and diluting a small volume just before use.
However our EM lab isn't very busy (3-4 preps every few months, although I am working on trying to get more users!) and I end up having to throw out a lot of the 20ml solution that I make; membranes start looking kind of crappy and with the older solution even if the solution still looks relatively clear. I also see a lot of black specks in my unstained sections.
I don't know of anyone who uses the premade ampoules, so I don't know if there are any significant differences in image quality. They come in such small volumes, which would be really convenient for me!
Thanks in advance!
-Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
-- Louie Kerr | Director, Microscopy and Research Services | Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543 508-289-7273 | lkerr-at-mbl.edu | www.mbl.edu
==============================Original Headers============================== 19, 41 -- From lkerr-at-mbl.edu Fri Sep 18 12:24:44 2015 19, 41 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu [128.128.172.17]) 19, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8IHOiqu008609 19, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 12:24:44 -0500 19, 41 -- Received: from mailfrontier.mbl.edu (127.0.0.1) id hvgvde0171sp for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 12:41:59 -0400 (envelope-from {lkerr-at-mbl.edu} ) 19, 41 -- Received: from mail.mbl.edu ([128.128.172.47]) 19, 41 -- by mailfrontier.mbl.edu (SonicWALL 8.0.8.3655) 19, 41 -- with ESMTP id 201509181641590079300; Fri, 18 Sep 2015 12:41:59 -0400 19, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 19, 41 -- by mail.mbl.edu (Postfix) with ESMTP id 9600F1824001 19, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 13:24:42 -0400 (EDT) 19, 41 -- Received: from mail.mbl.edu ([127.0.0.1]) 19, 41 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10032) 19, 41 -- with ESMTP id bgSi1cZViTc5 for {Microscopy-at-microscopy.com} ; 19, 41 -- Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 19, 41 -- by mail.mbl.edu (Postfix) with ESMTP id 792B71824003 19, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- X-Virus-Scanned: amavisd-new at mbl.edu 19, 41 -- Received: from mail.mbl.edu ([127.0.0.1]) 19, 41 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10026) 19, 41 -- with ESMTP id AIL96b8THi9A for {Microscopy-at-microscopy.com} ; 19, 41 -- Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- Received: from mail.mbl.edu (mail.mbl.edu [128.128.172.47]) 19, 41 -- by mail.mbl.edu (Postfix) with ESMTP id 5EA4D1824001 19, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- Date: Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- From: Louis Kerr {lkerr-at-mbl.edu} 19, 41 -- To: Microscopy-at-microscopy.com 19, 41 -- Message-ID: {1325508340.603417.1442597081288.JavaMail.zimbra-at-mbl.edu} 19, 41 -- In-Reply-To: {466682353.594958.1442591900484.JavaMail.zimbra-at-mbl.edu} 19, 41 -- Subject: premade aqueous OsO4 ampoules 19, 41 -- MIME-Version: 1.0 19, 41 -- Content-Type: text/plain; charset=utf-8 19, 41 -- Content-Transfer-Encoding: 7bit 19, 41 -- X-Originating-IP: [128.128.172.47] 19, 41 -- X-Mailer: Zimbra 8.0.9_GA_6191 (ZimbraWebClient - FF40 (Mac)/8.0.9_GA_6191) 19, 41 -- Thread-Topic: premade aqueous OsO4 ampoules 19, 41 -- Thread-Index: MopQGwIA7hLDGvEgmk653U3RyhfVCw== 19, 41 -- X-Mlf-Version: 8.0.8.3655 19, 41 -- X-Mlf-UniqueId: o201509181641590079300 ==============================End of - Headers==============================
I worked in biological microscopy for several years prior to a long career in an industrial polymer microscopy. In the bio lab, we invariably made our OsO4 solutions from crystalline OsO4. In the polymer microscopy lab, we rarely used OsO4, often in short bursts. Invariably, I was skeptical of the pre-made solutions because many of the vials went to waste as indicated by black precipitate on the inner surface of the vials and loss of amber color of the solution.
The issue, as you indicated, is quality versus price (through waste). To maintain high quality, I suggest you keep OsO4 crystals on hand and make fresh batches as needed. The price of the crystals is less than the prepared solution and you can be assured of the quality: Electron Microscopy Sciences sells 1 gram of OsO4 crystals for $32 versus $53 for 10, 2 ml vials of 4% OsO4 solution.
When you make up a batch OsO4 solution, break it up into several small vials and store each under a inert gas (nitrogen or argon) head to slow degradation of the oxide. When a vial begins to go bad, hopefully the unopened vials are still fresh(er) and usable.
For the reasons you mention we have been making use of the premade aqueous OsO4 solutions for years. It works well for our own use and it is convenient when another user comes by asking for a few ml's of OsO4. Occasionally we still make up a solution for odd ball recipes or large quantities.
Thanks, Louie
----- Original Message ----- X-from: bicarbaj-at-mtholyoke.edu To: lkerr-at-mbl.edu Sent: Friday, September 18, 2015 11:01:37 AM
Hello everyone,
Could others please share their experiences with the premade aqueous OsO4 solutions?
I have been making my own 4% solution, storing it in the fridge, and diluting a small volume just before use.
However our EM lab isn't very busy (3-4 preps every few months, although I am working on trying to get more users!) and I end up having to throw out a lot of the 20ml solution that I make; membranes start looking kind of crappy and with the older solution even if the solution still looks relatively clear. I also see a lot of black specks in my unstained sections.
I don't know of anyone who uses the premade ampoules, so I don't know if there are any significant differences in image quality. They come in such small volumes, which would be really convenient for me!
Thanks in advance!
-Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
-- Louie Kerr | Director, Microscopy and Research Services | Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543 508-289-7273 | lkerr-at-mbl.edu | www.mbl.edu
==============================Original Headers============================== 19, 41 -- From lkerr-at-mbl.edu Fri Sep 18 12:24:44 2015 19, 41 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu [128.128.172.17]) 19, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8IHOiqu008609 19, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 12:24:44 -0500 19, 41 -- Received: from mailfrontier.mbl.edu (127.0.0.1) id hvgvde0171sp for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 12:41:59 -0400 (envelope-from {lkerr-at-mbl.edu} ) 19, 41 -- Received: from mail.mbl.edu ([128.128.172.47]) 19, 41 -- by mailfrontier.mbl.edu (SonicWALL 8.0.8.3655) 19, 41 -- with ESMTP id 201509181641590079300; Fri, 18 Sep 2015 12:41:59 -0400 19, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 19, 41 -- by mail.mbl.edu (Postfix) with ESMTP id 9600F1824001 19, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 13:24:42 -0400 (EDT) 19, 41 -- Received: from mail.mbl.edu ([127.0.0.1]) 19, 41 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10032) 19, 41 -- with ESMTP id bgSi1cZViTc5 for {Microscopy-at-microscopy.com} ; 19, 41 -- Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 19, 41 -- by mail.mbl.edu (Postfix) with ESMTP id 792B71824003 19, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- X-Virus-Scanned: amavisd-new at mbl.edu 19, 41 -- Received: from mail.mbl.edu ([127.0.0.1]) 19, 41 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10026) 19, 41 -- with ESMTP id AIL96b8THi9A for {Microscopy-at-microscopy.com} ; 19, 41 -- Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- Received: from mail.mbl.edu (mail.mbl.edu [128.128.172.47]) 19, 41 -- by mail.mbl.edu (Postfix) with ESMTP id 5EA4D1824001 19, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- Date: Fri, 18 Sep 2015 13:24:41 -0400 (EDT) 19, 41 -- From: Louis Kerr {lkerr-at-mbl.edu} 19, 41 -- To: Microscopy-at-microscopy.com 19, 41 -- Message-ID: {1325508340.603417.1442597081288.JavaMail.zimbra-at-mbl.edu} 19, 41 -- In-Reply-To: {466682353.594958.1442591900484.JavaMail.zimbra-at-mbl.edu} 19, 41 -- Subject: premade aqueous OsO4 ampoules 19, 41 -- MIME-Version: 1.0 19, 41 -- Content-Type: text/plain; charset=utf-8 19, 41 -- Content-Transfer-Encoding: 7bit 19, 41 -- X-Originating-IP: [128.128.172.47] 19, 41 -- X-Mailer: Zimbra 8.0.9_GA_6191 (ZimbraWebClient - FF40 (Mac)/8.0.9_GA_6191) 19, 41 -- Thread-Topic: premade aqueous OsO4 ampoules 19, 41 -- Thread-Index: MopQGwIA7hLDGvEgmk653U3RyhfVCw== 19, 41 -- X-Mlf-Version: 8.0.8.3655 19, 41 -- X-Mlf-UniqueId: o201509181641590079300 ==============================End of - Headers==============================
--
"An expert is a person who has made all the mistakes which can be made in a very narrow field.â and "Prediction is very difficult, especially if it's about the future." Niels Bohr
==============================Original Headers============================== 38, 31 -- From microscopy.gmb-at-gmail.com Fri Sep 18 17:02:49 2015 38, 31 -- Received: from mail-ig0-f174.google.com (mail-ig0-f174.google.com [209.85.213.174]) 38, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8IM2ncI009275 38, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 17:02:49 -0500 38, 31 -- Received: by igxx6 with SMTP id x6so26601556igx.1 38, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Sep 2015 15:02:47 -0700 (PDT) 38, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 38, 31 -- d=gmail.com; s=20120113; 38, 31 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 38, 31 -- :content-type:content-transfer-encoding; 38, 31 -- bh=kz5FuFPuuN5R2vzYUPcE3d+fQSQ5PIEpz9SnPLZ3FMc=; 38, 31 -- b=MBd7XdTpzPRCqOJbaSY8HbzxcAbcNSQsm4u3UYlu+YoOlNVjK/7gcqKZJRGZR3KTZs 38, 31 -- Oglw9sJiRvLx+PV5yuIWO3LKrX9ZspmZCRjWL8NgfUrp9q75SS+ezHcB3w//pup6ExBL 38, 31 -- G16EyGDoETdpEpLuxgVeSXIULiP7ZbIPkmDpId8e+vcjd8DUas3NPLu/2j/GVm4osDZW 38, 31 -- UfeX/OVLZQb3Pz4QgKykAbseyasRj1gCXbe7VdZJ1AZKhu49ldwrEr6nBZALSAqe/1Us 38, 31 -- zJSRIN5rLwhp168jQVm4UVN6StcQooZp/PTnVcwdslhWcnyTwLX5WxOlBk21FlbD0Ftn 38, 31 -- ASpw== 38, 31 -- MIME-Version: 1.0 38, 31 -- X-Received: by 10.50.45.33 with SMTP id j1mr610508igm.61.1442613767595; Fri, 38, 31 -- 18 Sep 2015 15:02:47 -0700 (PDT) 38, 31 -- Received: by 10.107.176.15 with HTTP; Fri, 18 Sep 2015 15:02:47 -0700 (PDT) 38, 31 -- In-Reply-To: {201509181736.t8IHagbq026645-at-ns.microscopy.com} 38, 31 -- References: {201509181736.t8IHagbq026645-at-ns.microscopy.com} 38, 31 -- Date: Fri, 18 Sep 2015 17:02:47 -0500 38, 31 -- Message-ID: {CADhGOT+s+VcuRmgXLJvtxQZH7CqV+AgN2UW73S0fuJwt4Nj0TA-at-mail.gmail.com} 38, 31 -- Subject: Fwd: [Microscopy] premade aqueous OsO4 ampoules 38, 31 -- From: Gary Brown {microscopy.gmb-at-gmail.com} 38, 31 -- To: Listserver {Microscopy-at-microscopy.com} , bicarbaj-at-mtholyoke.edu 38, 31 -- Content-Type: text/plain; charset=UTF-8 38, 31 -- Content-Transfer-Encoding: 8bit 38, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8IM2ncI009275 ==============================End of - Headers==============================
There is one consideration you may wish to put on the balance in relation to the choice between making your own solutions or using premade dilute solutions in small vials. That is worker risk and safety. As I am sure you know, this chemical happens to be extremely and acutely toxic and can cause permanent blindness if it gets into the eye. As scientists, many of us work with some very dangerous and toxic compounds as part of our daily work. In our laboratory, we endeavor to reduce or mitigate risks anyway possible within reason. Our concentrated trace metal acids are purchased in small 500mL bottles by the case and we have dispensing units that go right into the bottle to allow direct delivery of the desired acid and avoid both contamination of the reagent and the risk of exposure from having a larger bottle of acid dropped or spilled during transfer. We use prepared pesticide solutions from certified vendors (ISO) as well as stock inorganic standards already prepared in solution at 10PPM/125mL bottles (far more pure than we could make them in the lab and certified too). When we purchase BF3 MeOH, TFA or TMCS as well as other highly reactive derivatization reagents, I buy them in 1mL x 10 ampoules so that there is never more than 1mL being used at one time. This does add cost to the laboratory operations but I find it worth it for our needs. All that said, it does not mean I fear dangerous or toxic chemicals but rather have great respect for them and find the added cost well worth the peace of mind. I am grateful I can make that choice and I realize not everyone can due to budget limitations.
We currently do not use OsO4 in our microscopy laboratory group but we may have need of it in the future. Should that be the case, I would probably purchase the prepared solutions in small ampoules unless we found the quality to be lacking. Sometimes one vendor's quality is far superior to another and that is worth investigating in this case due to differing experiences shared by other experienced users of OsO4.
I just wanted to bring safety considerations into the conversation because anytime one is dealing with acutely toxic compounds, safety and ways in which we can reduce risks in the lab deserve our consideration. This is especially true in educational environments where students are just learning the techniques.
Sincerely,
James Neal-Kababick Director Flora Research Laboratories, LLC An FDA and DEA Registered & Inspected Laboratory Fellow AOAC International President Elect, PSW-AOAC Vice Chair USP {2251} ADSDDA Expert Panel United States Pharmacopeia NBDS Expert Committee USP Joint Standards Setting Subcommittee for Reference Standards (JS3) Adjunct Faculty Bastyr University Botanical Medicine Department 1-541-472-0980 phone jimk-at-floraresearch.com www.floraresearch.com
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Hello everyone,
Could others please share their experiences with the premade aqueous OsO4 solutions?
I have been making my own 4% solution, storing it in the fridge, and diluting a small volume just before use.
However our EM lab isn't very busy (3-4 preps every few months, although I am working on trying to get more users!) and I end up having to throw out a lot of the 20ml solution that I make; membranes start looking kind of crappy and with the older solution even if the solution still looks relatively clear. I also see a lot of black specks in my unstained sections.
I don't know of anyone who uses the premade ampoules, so I don't know if there are any significant differences in image quality. They come in such small volumes, which would be really convenient for me!
Thanks in advance!
-Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
-- Louie Kerr | Director, Microscopy and Research Services | Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543 508-289-7273 | lkerr-at-mbl.edu | www.mbl.edu
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Email: gxn7-at-psu.edu Name: Greg Ning
Organization: Penn State University
Title-Subject: [Filtered] TEM Job -at- Penn State Hershey Med
Message: Transmission Electron Microscopist  Microscopy Imaging Facility The Pennsylvania State University College of Medicine, Hershey, Pennsylvania
The PSU College of Medicine seeks qualified applicants for a Transmission Electron Microscopist position within our Core Facility. The successful candidate will be responsible for all aspects of TEM sample preparation and image acquisition in a recently constructed 600 sq ft laboratory equipped with a JEOL 1400 TEM, plus all needed sectioning and other equipment. The TEM facility is part of our Microscopy Imaging Facility (http://www.pennstatehershey.org/web/core/microscopy-histology), which provides a range of microscopy imaging acquisition services (in addition to TEM) to PSU researchers, including CryoEM, Confocal, Multiphoton, and Deconvolution microscopy, and advanced image processing with AUTO3DEM, EMAN2, Bitplane IMARIS, VOLOCITY, and HUYGENS Deconvolution software. The Microscopy Imaging Facility currently occupies } 2000 square feet of space and is equipped with a JEOL 2100 Cryo-EM, a Leica SP8 White Light Laser Confocal, a DeltaVision Elite Deconvolution Microscope, and a Nikon A1MP+ Multiphoton Microscope System, in addition to the JEOL 1400 TEM.
Minimum qualifications for the position include a BachelorÂs Degree in Science (M.S. or Ph.D. degrees in a relevant field would be a plus) with extensive hands-on experience in transmission electron microscopy imaging, relevant sample preparation methods involving biological tissues and cell lines, negative staining, immune-labeling procedures, and preferably additional core facility management experience. The primary expertise required for the position will be in TEM microscopy and relevant sample preparation methods. Not required but advantageous would be additional experience in light microscopy and CryoEM methods. The successful candidate must show evidence of strong interpersonal communication skills as well as the ability to collaborate with others, as (s)he will be working with a wide variety of biomedical researchers to aid in the design and successful completion of diverse research projects. Grant writing experience is not required, but would be a plus in possible instrumentation grant preparation, working with researchers in our various departments and institutes (e.g., the Penn State Clinical and Translational Science Institute, the Penn State Hershey Cancer Institute, the Heart and Vascular Institute, and the Huck Institute of the Life Sciences).
Review of applications will begin immediately and continue until the position is filled. Salary will be commensurate with the candidateÂs experience and qualifications. Candidates should provide a single PDF packet containing a letter of application, a concise statement of relevant research experience in transmission electron microscopy methods and imaging, curriculum vitae, and names and contact information for three references. This should be sent to Dr. John W. Wills, Chair of the TEM search committee, at jww4-at-psu.edu. Candidates should also apply online at https://psu.jobs/job/58857.
The Pennsylvania State University College of Medicine is an equal opportunity, affirmative action employer. Women and minority candidates are especially encouraged to apply. For your health, we are a non-smoking campus.
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Many thanks to everybody who responded to my posting on doing TEM after SEM on the same sample. I got interesting responses from people they have done it -and also from people they have not done it. It looks like this SEM-} TEM combination is feasible but not very popular (I didn't understand very well why). Best wishes yorgos Dr Yorgos Nikas Athens Innovative Microscopy Skra 36 Voula 16673 GREECE
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} Name: Steven Hill } } School: Adele C. Young Intermediate School } } Grade/Education Level: Middle School } } Location: Brigham City, UT US } } Email: steven.hill-at-besd.net } } Subject: Microscope Preventive Maintenance } } Your Question: Our light microscopes have not had any form of maintenance in years. We are looking for someone in Utah who could work on our microscopes. If you know of any, We would appreciate it very much. }
==============================Original Headers============================== 3, 36 -- From oshel1pe-at-cmich.edu Mon Sep 21 14:18:47 2015 3, 36 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8LJIlKl030046 3, 36 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2015 14:18:47 -0500 3, 36 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 3, 36 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8LJIi4s009550 3, 36 -- for {microscopy-at-microscopy.com} ; Mon, 21 Sep 2015 15:18:44 -0400 3, 36 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 3, 36 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 3, 36 -- (TLS) id 14.3.248.2; Mon, 21 Sep 2015 15:18:44 -0400 3, 36 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 3, 36 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 3, 36 -- (TLS) id 14.3.248.2; Mon, 21 Sep 2015 15:18:43 -0400 3, 36 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 3, 36 -- cas2.central.cmich.local (141.209.15.141) with Microsoft SMTP Server (TLS) id 3, 36 -- 14.3.248.2; Mon, 21 Sep 2015 15:18:43 -0400 3, 36 -- Message-ID: {56005813.5090606-at-cmich.edu} 3, 36 -- Date: Mon, 21 Sep 2015 15:18:43 -0400 3, 36 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 36 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 36 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 36 -- MIME-Version: 1.0 3, 36 -- To: micro {microscopy-at-microscopy.com} 3, 36 -- Subject: Re: Ask a Microscopist - School Microscope Preventive Maintenance 3, 36 -- in Utah 3, 36 -- References: {83190522.2799.1442704771615.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 3, 36 -- In-Reply-To: {83190522.2799.1442704771615.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 3, 36 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 36 -- Content-Transfer-Encoding: 7bit 3, 36 -- X-Originating-IP: [141.209.2.100] 3, 36 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 36 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 36 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 3, 36 -- X-CanItPRO-Stream: default 3, 36 -- X-Canit-Stats-ID: 02PjTiIXc - 629035c4e87e - 20150921 3, 36 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I am looking for mineral (sub-micro) particles in mouse organs by TEM and at the same time I am trying to localize them by LM in dark field mode in semi-thin sections (300nm). Since they have a size under 1ľm I just hope that I can find them at all in LM in dark field mode but this is another story. At the moment my problem is the dirt on the glass slides. I tried to clean the glass slides with detergent, sonication and ethanol but I still have too much dirt in the background.
Does everybody have a proven protocol and want to share it with me? Thank you in advance.
Stephane
==============================Original Headers============================== 5, 36 -- From nizets2-at-yahoo.com Tue Sep 22 05:49:47 2015 5, 36 -- Received: from nm48-vm5.bullet.mail.ne1.yahoo.com (nm48-vm5.bullet.mail.ne1.yahoo.com [98.138.121.117]) 5, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8MAnk3u010727 5, 36 -- for {microscopy-at-microscopy.com} ; Tue, 22 Sep 2015 05:49:47 -0500 5, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1442918984; bh=p4dCGhr1cWPUo1YX2kIIWlqFLg+GoZhK/yvvB/Laqic=; h=Date:From:Subject:To:From:Subject; b=Cw4OROIKn9hHJfGRhE0o0PYXaXwo4GtVHvap9KqciaPnPB0Q5U/iwz/imgjo4I0PZE/17R096lPA+aXkWADulLED00rUs7eO9+5uIP2z+OdXn32iTLvQne6xotFi4fWt8fGGMZ+8K6PNPeh07PoaVvwz6N/Egr/ZDdKwe9B5HWmS8ti1OSIiamIjz4vFVYqA0k3zc0hgt3HHUhE0qpSyAwwF0yUnXlGn8OAhiuACMUudhuSngkHzGEaHb8Uj5K2E5BWmJKkcvz2o3B40/c30pDqbccDzl451HPuExin52fR6xQkvgcqa95XGN+TythKsXwLLtoUgw84rBjBmsxpjnw== 5, 36 -- Received: from [127.0.0.1] by nm48.bullet.mail.ne1.yahoo.com with NNFMP; 22 Sep 2015 10:49:44 -0000 5, 36 -- Received: from [98.138.101.132] by nm48.bullet.mail.ne1.yahoo.com with NNFMP; 22 Sep 2015 10:46:59 -0000 5, 36 -- Received: from [98.139.215.143] by tm20.bullet.mail.ne1.yahoo.com with NNFMP; 22 Sep 2015 10:46:59 -0000 5, 36 -- Received: from [98.139.212.192] by tm14.bullet.mail.bf1.yahoo.com with NNFMP; 22 Sep 2015 10:46:59 -0000 5, 36 -- Received: from [127.0.0.1] by omp1001.mail.bf1.yahoo.com with NNFMP; 22 Sep 2015 10:46:59 -0000 5, 36 -- X-Yahoo-Newman-Property: ymail-4 5, 36 -- X-Yahoo-Newman-Id: 212473.78780.bm-at-omp1001.mail.bf1.yahoo.com 5, 36 -- Received: (qmail 83622 invoked by uid 60001); 22 Sep 2015 10:46:59 -0000 5, 36 -- X-YMail-OSG: B1mVAeQVM1k0pJ2rE.fu42aHL2M8CRYxN.lMURfYvzYoZti 5, 36 -- uvfY_KyqBhOCKzFpyHbQxgbDdsFzULwWxI4aMxUpy0.469Zm34ONWMGr2oIO 5, 36 -- dn6LLQbcCWytjbVFScZVmtqyNQbCGFwFQ24VtDD5rpGqssfIk1WR7hhVR90o 5, 36 -- AKoVgBE.f9x9bbyclmdxMMkyYDTUH_WA.JJmswlStsTmOt8yt0C6m7x_tkMO 5, 36 -- qOze69YwMyXQkrvv_nfyJbW5b1Zrpylos9gd.PCH95W6NLU3fYAc2JDIPVVu 5, 36 -- WYLH2fNcO2Tvmz9xbdkSWFbLYSHEuNj7yaspwepTmB0I2kOZcswrigeJZmZ5 5, 36 -- GT6z.Izr1K7t5k8rc8UkBgOXKwc74d1TseKXt_D65rnZN3bpktv.e69YT6th 5, 36 -- MQazENgbu1g6G7mRXeIr2onbUsUfgqqrAbPKiwFaZknG6b7KGakSrRj1idtD 5, 36 -- yILbmKvSpGqIoMTmpl3AeI_YThrJgkT0rIaMswJDS0ZdTlMa0pXKa2xNyWfa 5, 36 -- 2OnI7l_yD3.piZv9GF_qBBlngBAiYsBL8GN7Ju_Q0u12qtG9KAKpuUNM2sDb 5, 36 -- S3xhxScji7jMxZ0w- 5, 36 -- Received: from [213.33.126.84] by web141704.mail.bf1.yahoo.com via HTTP; Tue, 22 Sep 2015 03:46:58 PDT 5, 36 -- X-Rocket-MIMEInfo: 002.001,RGVhciBMaXN0ZXJzLA0KDQpJIGFtIGxvb2tpbmcgZm9yIG1pbmVyYWwgKHN1Yi1taWNybykgcGFydGljbGVzIGluIG1vdXNlIG9yZ2FucyBieSBURU0gYW5kIGF0IHRoZSBzYW1lIHRpbWUgSSBhbSB0cnlpbmcgdG8gbG9jYWxpemUgdGhlbSBieSBMTSBpbiBkYXJrIGZpZWxkIG1vZGUgaW4gc2VtaS10aGluIHNlY3Rpb25zICgzMDBubSkuDQpTaW5jZSB0aGV5IGhhdmUgYSBzaXplIHVuZGVyIDHCtW0gSSBqdXN0IGhvcGUgdGhhdCBJIGNhbiBmaW5kIHRoZW0gYXQgYWxsICBpbiBMTSBpbiBkYXJrIGZpZWxkIG0BMAEBAQE- 5, 36 -- X-Mailer: YahooMailBasic/701 YahooMailWebService/0.8.203.817 5, 36 -- Message-ID: {1442918818.39021.YahooMailBasic-at-web141704.mail.bf1.yahoo.com} 5, 36 -- Date: Tue, 22 Sep 2015 03:46:58 -0700 5, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 36 -- Subject: clean glass slides for dark field microscopy 5, 36 -- To: microscopy-at-microscopy.com 5, 36 -- MIME-Version: 1.0 5, 36 -- Content-Type: text/plain; charset=iso-8859-1 5, 36 -- Content-Transfer-Encoding: 8bit 5, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8MAnk3u010727 ==============================End of - Headers==============================
Apologies for coming back, I got some more good comments and would like to conclude: With SEM-} TEM you get a global idea of the surface morphology of the tissue piece and maybe, if you orient the specimen welll, of the particular section you look in TEM and this is very important. The cost is that you loose in TEM quality and also, it requires a high level of collaboration between microscopist, investigator and technician to see all its benefits. Cheers yorgos
----- Original Message ----- X-from: {eikonika-at-otenet.gr} To: {eikonika-at-otenet.gr} Sent: Monday, September 21, 2015 7:48 PM
Dear Listers,
I have objectives on a Zeiss axioimager light microscope with a label "HD" and I am wondering what that means. I can't find the information in the internet. Most of the info related to optics refers to high-definition of course, which makes the search quite hard. I suspect that it means "Hellfeld/Dunkelfeld" (bright field/dark field) but I am not sure. If it is the case, what makes these objectives so special? What is the feature which makes them more "dark field efficient"?
Regards Stephane
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From steven.345345.f-at-gmail.com Tue Sep 22 09:11:07 2015 Return-Path: {steven.345345.f-at-gmail.com} Received: from gmail.com ([220.241.216.221]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8MEB39K015411 for {microscopylistserverarchive5-at-microscopy.com} ; Tue, 22 Sep 2015 09:11:05 -0500 Reply-To: steven.345345.f-at-gmail.com
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Hello everyone,
Iâve been trying to develop a procedure to clean our STEM samples prior to insertion into our ARM200CF microscope. In particular, we are trying to perform highly analytical work that involves long EELS / EDS mapping, so we would like to reduce hydrocarbon contamination on the surface of our samples.
Since most of our specimens are oxide lift outs, they are quite robust to heating and plasma cleaning. Typically after a lift out is completed I will plasma clean for 2-5 minutes prior to insertion; however, someone recently suggested baking our samples for a few hours at 50-100 ÂşC to further reduce contamination. It was also suggested that we place our heater in a bell jar-like setup so that we can pump on the sample during the annealing.
I am considering building such a setup, but I would like to see if anyone has constructed something like this before. I would very much appreciate any design suggestions or other tips to help us clean up our samples.
Thank you! ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
Hi Steve, It definitely helps to bake - and not be shy about it. 160C for 8-12 hours (i.e. overnight) is good. I find that if that doesn't do it, it won't come clean. Our setup is not exactly home-made, but it consists of a Pfeiffer HiCube dry pumping station and an MTI quartz tube furnace. That way it is possible to do vacuum or air or to introduce other gas environments. And the temperature vs. time profile is programmable. I can send you a picture if you're interested. May be overkill for you... Regards, Larry
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: Tuesday, September 22, 2015 2:03 PM To: LES-at-ZSGENETICS.COM
Hello everyone,
IââŹâ˘ve been trying to develop a procedure to clean our STEM samples prior to insertion into our ARM200CF microscope. In particular, we are trying to perform highly analytical work that involves long EELS / EDS mapping, so we would like to reduce hydrocarbon contamination on the surface of our samples.
Since most of our specimens are oxide lift outs, they are quite robust to heating and plasma cleaning. Typically after a lift out is completed I will plasma clean for 2-5 minutes prior to insertion; however, someone recently suggested baking our samples for a few hours at 50-100 ĂÂşC to further reduce contamination. It was also suggested that we place our heater in a bell jar-like setup so that we can pump on the sample during the annealing.
I am considering building such a setup, but I would like to see if anyone has constructed something like this before. I would very much appreciate any design suggestions or other tips to help us clean up our samples.
Thank you! ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
As a matter of curiosity, what is the consensus on the usable lifetime of a made up from crystal, 4% osmium in water stock solution, stored in a fridge?
While on the subject of osmium stock solution storage, do people clean their stock solution storage bottle between making up their osmium stock solutions?
Have a great day
Allan
On 19/09/2015, at 3:10 AM, {bicarbaj-at-mtholyoke.edu} {bicarbaj-at-mtholyoke.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello everyone, } } Could others please share their experiences with the premade aqueous } OsO4 solutions? } } I have been making my own 4% solution, storing it in the fridge, and } diluting a small volume just before use. } } However our EM lab isn't very busy (3-4 preps every few months, } although I am working on trying to get more users!) and I end up } having to throw out a lot of the 20ml solution that I make; membranes } start looking kind of crappy and with the older solution even if the } solution still looks relatively clear. I also see a lot of black } specks in my unstained sections. } } I don't know of anyone who uses the premade ampoules, so I don't know } if there are any significant differences in image quality. They come } in such small volumes, which would be really convenient for me! } } Thanks in advance! } } -Blanca }
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254 EM Centre: http://ocem.otago.ac.nz/
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254 EM Centre: http://ocem.otago.ac.nz/
==============================Original Headers============================== 20, 22 -- From allan.mitchell-at-stonebow.otago.ac.nz Tue Sep 22 15:35:16 2015 20, 22 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 20, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8MKZF0b017945 20, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 22 Sep 2015 15:35:16 -0500 20, 22 -- Received: from anatomy.otago.ac.nz (anatomyy.otago.ac.nz [139.80.40.227]) 20, 22 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id t8MKZCdM011634 20, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Sep 2015 08:35:12 +1200 20, 22 -- Received: from ou040139.otago.ac.nz (ou040139.otago.ac.nz [139.80.40.139]) 20, 22 -- by anatomy.otago.ac.nz (Postfix) with ESMTP id AE190D0CF1C4 20, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Sep 2015 08:35:12 +1200 (NZST) 20, 22 -- Content-Type: text/plain; charset=us-ascii 20, 22 -- Mime-Version: 1.0 (Apple Message framework v1084) 20, 22 -- Subject: Re: [Microscopy] premade aqueous OsO4 ampoules 20, 22 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 20, 22 -- In-Reply-To: {201509181510.t8IFA5AB031579-at-ns.microscopy.com} 20, 22 -- Date: Wed, 23 Sep 2015 08:35:12 +1200 20, 22 -- Message-Id: {7BA1A104-7209-4582-9F69-5B9DC30C1034-at-stonebow.otago.ac.nz} 20, 22 -- References: {201509181510.t8IFA5AB031579-at-ns.microscopy.com} 20, 22 -- To: {Microscopy-at-Microscopy.Com} 20, 22 -- X-Mailer: Apple Mail (2.1084) 20, 22 -- Content-Transfer-Encoding: 8bit 20, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8MKZF0b017945 ==============================End of - Headers==============================
I've not seen yet (I don't think?) any mention of freezing the Osmium samples. Is there a reason for this?
In this facility, I make up fifty 1ml aliquots of 2% aqueous osmium, from 1g osmium crystals, into clean 7ml flat bottom glass vials with PP screw cap lids with foil inserts (to prevent splashes and vapour seepage), and store them in the -20 freezer in double Tupperware boxes (one inside another - for extra safety) only taking out the required number of vials for each processing session. As they are such small quantities, they are safer to handle and defrost quickly. I have found that I can store the osmium in this way for years without noticing significant artefact.
I would also be interested to hear how people neutralise their osmium after use?
Thanks
Nat
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Hi All
As a matter of curiosity, what is the consensus on the usable lifetime of a made up from crystal, 4% osmium in water stock solution, stored in a fridge?
While on the subject of osmium stock solution storage, do people clean their stock solution storage bottle between making up their osmium stock solutions?
Have a great day
Allan
On 19/09/2015, at 3:10 AM, {bicarbaj-at-mtholyoke.edu} {bicarbaj-at-mtholyoke.edu} wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello everyone, } } Could others please share their experiences with the premade aqueous } OsO4 solutions? } } I have been making my own 4% solution, storing it in the fridge, and } diluting a small volume just before use. } } However our EM lab isn't very busy (3-4 preps every few months, } although I am working on trying to get more users!) and I end up } having to throw out a lot of the 20ml solution that I make; membranes } start looking kind of crappy and with the older solution even if the } solution still looks relatively clear. I also see a lot of black } specks in my unstained sections. } } I don't know of anyone who uses the premade ampoules, so I don't know } if there are any significant differences in image quality. They come } in such small volumes, which would be really convenient for me! } } Thanks in advance! } } -Blanca }
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254 EM Centre: http://ocem.otago.ac.nz/
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254 EM Centre: http://ocem.otago.ac.nz/
==============================Original Headers============================== 20, 22 -- From allan.mitchell-at-stonebow.otago.ac.nz Tue Sep 22 15:35:16 2015 20, 22 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 20, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8MKZF0b017945 20, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 22 Sep 2015 15:35:16 -0500 20, 22 -- Received: from anatomy.otago.ac.nz (anatomyy.otago.ac.nz [139.80.40.227]) 20, 22 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id t8MKZCdM011634 20, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Sep 2015 08:35:12 +1200 20, 22 -- Received: from ou040139.otago.ac.nz (ou040139.otago.ac.nz [139.80.40.139]) 20, 22 -- by anatomy.otago.ac.nz (Postfix) with ESMTP id AE190D0CF1C4 20, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Sep 2015 08:35:12 +1200 (NZST) 20, 22 -- Content-Type: text/plain; charset=us-ascii 20, 22 -- Mime-Version: 1.0 (Apple Message framework v1084) 20, 22 -- Subject: Re: [Microscopy] premade aqueous OsO4 ampoules 20, 22 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 20, 22 -- In-Reply-To: {201509181510.t8IFA5AB031579-at-ns.microscopy.com} 20, 22 -- Date: Wed, 23 Sep 2015 08:35:12 +1200 20, 22 -- Message-Id: {7BA1A104-7209-4582-9F69-5B9DC30C1034-at-stonebow.otago.ac.nz} 20, 22 -- References: {201509181510.t8IFA5AB031579-at-ns.microscopy.com} 20, 22 -- To: {Microscopy-at-Microscopy.Com} 20, 22 -- X-Mailer: Apple Mail (2.1084) 20, 22 -- Content-Transfer-Encoding: 8bit 20, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8MKZF0b017945 ==============================End of - Headers==============================
Dear Fellow Microscopists, Has anybody there prepared their own holey carbon film supports? We usually use the film on Cu mesh grids. How is the mesh grid manufactured? What if I need it on a polymer grid? How do you control the size of the holes? What is the range of the holes we can achieve? Very grateful if you can share with me some pros and cons. Zhaoxia
Dr Zhaoxia Zhou Loughborough University, UK
==============================Original Headers============================== 4, 56 -- From Z.Zhou-at-lboro.ac.uk Wed Sep 23 04:28:15 2015 4, 56 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 4, 56 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8N9SFLQ021776 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 04:28:15 -0500 4, 56 -- Received: from [158.125.167.11] (helo=ITSCASH-4.lunet.lboro.ac.uk) 4, 56 -- by mta-1.lboro.ac.uk with esmtps (TLSv1:AES128-SHA:128) 4, 56 -- (Exim 4.80) 4, 56 -- id 1ZegLL-0001vz-Id 4, 56 -- for Microscopy-at-microscopy.com; Wed, 23 Sep 2015 10:28:03 +0100 4, 56 -- Received: from emea01-am1-obe.outbound.protection.outlook.com (213.199.154.13) 4, 56 -- by email.lboro.ac.uk (158.125.167.4) with Microsoft SMTP Server (TLS) id 4, 56 -- 14.3.235.1; Wed, 23 Sep 2015 10:28:08 +0100 4, 56 -- Received: from DB4PR04MB0623.eurprd04.prod.outlook.com (10.242.221.148) by 4, 56 -- DB4PR04MB0622.eurprd04.prod.outlook.com (10.242.221.147) with Microsoft SMTP 4, 56 -- Server (TLS) id 15.1.274.16; Wed, 23 Sep 2015 09:28:02 +0000 4, 56 -- Received: from DB4PR04MB0623.eurprd04.prod.outlook.com ([10.242.221.148]) by 4, 56 -- DB4PR04MB0623.eurprd04.prod.outlook.com ([10.242.221.148]) with mapi id 4, 56 -- 15.01.0274.009; Wed, 23 Sep 2015 09:28:02 +0000 4, 56 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} 4, 56 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 56 -- Subject: How to make holey carbon support film myself? 4, 56 -- Thread-Topic: How to make holey carbon support film myself? 4, 56 -- Thread-Index: AdD14ieYBGnC+BzSTsiNU9Qus0r6Vw== 4, 56 -- Date: Wed, 23 Sep 2015 09:28:02 +0000 4, 56 -- Message-ID: {DB4PR04MB0623096D5BE85E879ED3FEBDC6440-at-DB4PR04MB0623.eurprd04.prod.outlook.com} 4, 56 -- Accept-Language: en-GB, en-US 4, 56 -- Content-Language: en-US 4, 56 -- X-MS-Has-Attach: 4, 56 -- X-MS-TNEF-Correlator: 4, 56 -- authentication-results: spf=none (sender IP is ) 4, 56 -- smtp.mailfrom=Z.Zhou-at-lboro.ac.uk; 4, 56 -- x-originating-ip: [2001:630:301:3064:4022:187f:4b6f:6994] 4, 56 -- x-microsoft-exchange-diagnostics: 1;DB4PR04MB0622;5:uJlK9jCnc9XFG++/g38depDLd07D+aJQcobk29HqrT6II6OWq496RMfYHzIa3850H/9KkFeGFpzoVv4huusmixy4dPSyF0VBUK/UqiiljfbNZh414EOI3nXn/CP5kEz+/EyoYRCB2k7kjlNjtVEWvw==;24:AHd9oEJdTFEgz9MH3VlgR7THDQxD+aY7K0Pp9JNIWawLw3/oQGOr3zZKqkcBDb38j+l+A4Esm2cPmed4V2l1TVouryaLiEGMltIYbEyC5Cw=;20:TFxtI1hOKqR9Doo4UU69LWByf6t/KxhEajROSN6DddEC5tjMFfA/bj0B9JfyWldbxxFprA+pDm/J1Ql7NnurNA== 4, 56 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:DB4PR04MB0622; 4, 56 -- x-microsoft-antispam-prvs: {DB4PR04MB0622B4A9818FD335CC72B768C6440-at-DB4PR04MB0622.eurprd04.prod.outlook.com} 4, 56 -- x-exchange-antispam-report-test: UriScan:; 4, 56 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(5005006)(8121501046)(520078)(3002001);SRVR:DB4PR04MB0622;BCL:0;PCL:0;RULEID:;SRVR:DB4PR04MB0622; 4, 56 -- x-forefront-prvs: 07083FF734 4, 56 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(189002)(229853001)(122556002)(74482002)(40100003)(110136002)(33656002)(106356001)(74316001)(86362001)(102836002)(77096005)(189998001)(107886002)(5003600100002)(68736005)(76576001)(101416001)(50986999)(11100500001)(5004730100002)(5007970100001)(5002640100001)(87936001)(2501003)(81156007)(54356999)(5001830100001)(77156002)(450100001)(105586002)(92566002)(46102003)(64706001)(2900100001)(2351001)(4001540100001)(10400500002)(5001960100002)(5001920100001)(62966003)(97736004)(5001860100001)(3826002);DIR:OUT;SFP:1102;SCL:1;SRVR:DB4PR04MB0622;H:DB4PR04MB0623.eurprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 4, 56 -- received-spf: None (protection.outlook.com: lboro.ac.uk does not designate 4, 56 -- permitted sender hosts) 4, 56 -- spamdiagnosticoutput: 1:23 4, 56 -- spamdiagnosticmetadata: NSPM 4, 56 -- Content-Type: text/plain; charset="utf-8" 4, 56 -- MIME-Version: 1.0 4, 56 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 23 Sep 2015 09:28:02.7863 4, 56 -- (UTC) 4, 56 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 4, 56 -- X-MS-Exchange-CrossTenant-id: cf264fc0-aeb8-449f-9054-82ce4454084b 4, 56 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DB4PR04MB0622 4, 56 -- X-OriginatorOrg: lboro.ac.uk 4, 56 -- X-Scan-Signature: ff8ace99f0b4d72f10e75a72ae25a6b7 4, 56 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 4, 56 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Wed, 23 Sep 2015 10:28:12 +0100 4, 56 -- Content-Transfer-Encoding: 8bit 4, 56 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t8N9SFLQ021776 ==============================End of - Headers==============================
I always reduced osmium tetroxide and ruthenium tetroxide to their dioxides using a sodium bisulfite solution (10 wt/vol %). Add an excess of the bisulfite solution into the vial of the tetroxide and leave for a while, an hour or more. This works well.
Some labs reduced osmium tetroxide with unsaturated vegetable oil.
Regards,
Gary M Brown Polymer Microscopy Consultant
--
"An expert is a person who has made all the mistakes which can be made in a very narrow field.â and "Prediction is very difficult, especially if it's about the future." Niels Bohr
==============================Original Headers============================== 8, 29 -- From microscopy.gmb-at-gmail.com Wed Sep 23 11:31:26 2015 8, 29 -- Received: from mail-ig0-f182.google.com (mail-ig0-f182.google.com [209.85.213.182]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8NGVQuk031476 8, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 11:31:26 -0500 8, 29 -- Received: by igbni9 with SMTP id ni9so31369620igb.0 8, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 09:31:25 -0700 (PDT) 8, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 29 -- d=gmail.com; s=20120113; 8, 29 -- h=mime-version:date:message-id:subject:from:to:content-type 8, 29 -- :content-transfer-encoding; 8, 29 -- bh=WAAC/YKUs9Vd8POTvmMmt9IMQUl6teAVZWuWERdpYds=; 8, 29 -- b=sXoajiLVdqDErtFM9Fm5w2ArBqoZDnbPx3TPSjoJOITjnNWe+AxJsaMquUGCP92GOy 8, 29 -- JuKCulFAIfFbIzy79xkl4qbymWhjRsousQNGHjxFykfLoSH92aJ2BoYos02fF1pptQ6J 8, 29 -- wHcYrEd0/ae7tvYl418W20En0xPlrXgOr4tX3lAMUI1GICyO97P9NP1Mij2AVQ/gWoXC 8, 29 -- j9j2f04r+tKT7670kFVfMRcr6egIh5nMgutKDRpYihTo/WW1/ZbhvpFvqnJ7hRDK7Ftr 8, 29 -- zoLBQ2sg/TFlI46ilDdV69MSOw3LajpnOCc/N2pS+B+cIDy86tEpXkh7NCPbClN2sgN/ 8, 29 -- zIaA== 8, 29 -- MIME-Version: 1.0 8, 29 -- X-Received: by 10.50.143.2 with SMTP id sa2mr8262615igb.21.1443025884950; Wed, 8, 29 -- 23 Sep 2015 09:31:24 -0700 (PDT) 8, 29 -- Received: by 10.107.176.15 with HTTP; Wed, 23 Sep 2015 09:31:24 -0700 (PDT) 8, 29 -- Date: Wed, 23 Sep 2015 11:31:24 -0500 8, 29 -- Message-ID: {CADhGOT+tYrgBfc8szkLL=sfS4ManrzjF9T8D2CADgsqFu0DXvA-at-mail.gmail.com} 8, 29 -- Subject: Neutralization (reduction) of osmium tetroxide 8, 29 -- From: Gary Brown {microscopy.gmb-at-gmail.com} 8, 29 -- To: Listserver {Microscopy-at-microscopy.com} 8, 29 -- Content-Type: text/plain; charset=UTF-8 8, 29 -- Content-Transfer-Encoding: 8bit 8, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8NGVQuk031476 ==============================End of - Headers==============================
Hey Microscopists, For those of you teaching microscopy courses, it can be challenging to explain the field and aperture diaphragm and how they effect the cone of light emerging from the condenser. On a whim, I filled a cuvette with fluorescein in order to visualize the cone of light. The demonstration proved quite successful, so I made a movie of the demonstration, showing how the field diaphragm affects the width of the cone without impacting its angle, and the aperture diaphragm impacts the angle of the cone without affecting its width. Having the students see the cone also went a long way in helping them understand why axial resolution goes down with a lower NA.
Here is the link to the movie: https://youtu.be/06CQ6IIaDWs
The cuvette also allowed me to show the hollow cone generated in phase contrast and darkfield: http://imgur.com/hswMRjH http://imgur.com/Zl87dcr
The sectored ray in oblique illumination: http://imgur.com/oerMkk0
As well as the solid cone in brightfield: http://imgur.com/KZP3Sv0 http://imgur.com/akeiI0T
Small disclaimer, the oblique was a "poor man's" oblique done my misaligning the darkfield annulus and partially closing the aperture diaphragm, but I also like to show "poor man's" oblique to students to show how thinking a little outside the box can allow you to get the most out of your microscope.
Hope this helps, Ben Smith
Benjamin E. Smith, Ph.D. Samuel Roberts Noble Microscopy Laboratory Research Scientist, Confocal Facility Manager University of Oklahoma Norman, OK 73019 E-mail: benjamin.smith-at-ou.edu Voice 405-325-4391 FAX 405-325-7619 http://www.microscopy.ou.edu/
==============================Original Headers============================== 8, 42 -- From prvs=070869321a=benjamin.smith-at-ou.edu Wed Sep 23 16:49:11 2015 8, 42 -- Received: from smtp5.ouhsc.edu (smtp5.ouhsc.edu [157.142.11.66]) 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8NLnBj5007601 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 16:49:11 -0500 8, 42 -- Received: from pps.filterd (smtp5.ouhsc.edu [127.0.0.1]) 8, 42 -- by smtp5.ouhsc.edu (8.15.0.59/8.14.7) with SMTP id t8NLmMx4030787 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 16:49:09 -0500 8, 42 -- Received: from matador.hsc.net.ou.edu (matador.hsc.net.ou.edu [10.26.192.106]) 8, 42 -- by smtp5.ouhsc.edu with ESMTP id 1x3jh2b2e4-1 8, 42 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 16:49:09 -0500 8, 42 -- Received: from IT-HAILSTORM.sooner.net.ou.edu (10.254.250.75) by 8, 42 -- MATADOR.hsc.net.ou.edu (10.26.192.106) with Microsoft SMTP Server (TLS) id 8, 42 -- 14.3.248.2; Wed, 23 Sep 2015 16:49:03 -0500 8, 42 -- Received: from IT-AIRSTORM.sooner.net.ou.edu ([fe80::7c13:a719:3a9c:9c52]) by 8, 42 -- IT-HAILSTORM.sooner.net.ou.edu ([::1]) with mapi id 14.03.0181.006; Wed, 23 8, 42 -- Sep 2015 16:49:04 -0500 8, 42 -- From: "Smith, Benjamin E." {benjamin.smith-at-ou.edu} 8, 42 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 42 -- Subject: LM Demonstrating the field and aperture diaphragm as well as 8, 42 -- darkfield illumination 8, 42 -- Thread-Topic: LM Demonstrating the field and aperture diaphragm as well as 8, 42 -- darkfield illumination 8, 42 -- Thread-Index: AdD2SamutE6NSXPrSFSao0PySwufKA== 8, 42 -- Date: Wed, 23 Sep 2015 21:49:03 +0000 8, 42 -- Message-ID: {9AEFBE3ACCC5714D94A0C4522016060701505DCE27-at-IT-AIRSTORM.sooner.net.ou.edu} 8, 42 -- Accept-Language: en-US 8, 42 -- Content-Language: en-US 8, 42 -- X-MS-Has-Attach: 8, 42 -- X-MS-TNEF-Correlator: 8, 42 -- x-originating-ip: [129.15.161.16] 8, 42 -- Content-Type: text/plain; charset="us-ascii" 8, 42 -- MIME-Version: 1.0 8, 42 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.14.151,1.0.33,0.0.0000 8, 42 -- definitions=2015-09-23_09:2015-09-23,2015-09-23,1970-01-01 signatures=0 8, 42 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 kscore.is_bulkscore=0 8, 42 -- kscore.compositescore=1 compositescore=0.9 suspectscore=0 phishscore=0 8, 42 -- bulkscore=0 kscore.is_spamscore=0 rbsscore=0.9 spamscore=0 8, 42 -- urlsuspectscore=0.9 adultscore=0 classifier=spam adjust=0 reason=mlx 8, 42 -- scancount=1 engine=7.0.1-1508030000 definitions=main-1509230293 8, 42 -- Content-Transfer-Encoding: 8bit 8, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8NLnBj5007601 ==============================End of - Headers==============================
} On Sep 23, 2015, at 2:40 AM, Z.Zhou-at-lboro.ac.uk wrote: } } } Dear Fellow Microscopists, } Has anybody there prepared their own holey carbon film supports? } We usually use the film on Cu mesh grids. How is the mesh grid manufactured? What if I need it on a polymer grid? } How do you control the size of the holes? What is the range of the holes we can achieve? } Very grateful if you can share with me some pros and cons. } Zhaoxia } } Dr Zhaoxia Zhou } Loughborough University, UK } } Dear Zhaoxia, I have done so, and it is trickier than making films without holes. There are several protocols that work, but it may take some time to develop the necessary skills. I found the trickiest part to be the separation of the holey formvar from the glass slide. Commonly, the oil from the skin of oneâs nose is used to coat the slide before dipping in formvar; however, in my case this oil did not workâit did work for former without holes. A solution of Apiazon L in petroleum ether did work for holey formvar. Another difficulty was that separating the film from the slide did not work when the humidity in the lab was high. I will let others answer your other questions. Yours, Bill
==============================Original Headers============================== 6, 33 -- From wtivol-at-sbcglobal.net Wed Sep 23 16:54:37 2015 6, 33 -- Received: from nm9-vm1.access.bullet.mail.bf1.yahoo.com (nm9-vm1.access.bullet.mail.bf1.yahoo.com [216.109.114.192]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8NLsZ2W011292 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 16:54:35 -0500 6, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1443045273; bh=bhrEtY/GmZpAyyyg0/0cqdOV2ZwR6vYXSIbRd8CX2xM=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=Hbk9rE664N4dHQhabzyAr5pOfYZg03ni/2J3W86ZmdLeMwS3UBA0fVnTgH9ROY1BCYSy4aj4QOi5bI4S5rFCZ3PVS6ulunqZTPOitz3z6yqJ8iGG1Xxdf8MV6a5/SHAWD7YTB2x/8k+KU1XS5HGSBSnr2yYqbfeCvvxh5hVsT1KM0AUGt5xWBqXVgQaNEpf0R6Vq46mi805C09iNZsLcNJDWTAEsqlqznTwVXhAltpNR39nHED3mZO5fh72Tu2jyzOq5sRXxtl0dTNsej3Mdf3Aw6bX/WrPRpA8gKhldVMkMH3tkMd7Se2vy26MqvXhy9uNYrCc39QhKrT/vscObVQ== 6, 33 -- Received: from [66.196.81.162] by nm9.access.bullet.mail.bf1.yahoo.com with NNFMP; 23 Sep 2015 21:54:33 -0000 6, 33 -- Received: from [98.139.244.52] by tm8.access.bullet.mail.bf1.yahoo.com with NNFMP; 23 Sep 2015 21:54:33 -0000 6, 33 -- Received: from [127.0.0.1] by smtp114.sbc.mail.bf1.yahoo.com with NNFMP; 23 Sep 2015 21:54:33 -0000 6, 33 -- X-Yahoo-Newman-Id: 255371.97997.bm-at-smtp114.sbc.mail.bf1.yahoo.com 6, 33 -- X-Yahoo-Newman-Property: ymail-3 6, 33 -- X-YMail-OSG: rYAS9U8VM1l.kFXuUZ3ebJuIxWFlnN34vpPCJAI1hngvlAQ 6, 33 -- bGfNwF8SheKl5A4dj7549Q2zLdmg6k._fMex9uwiOTANNLmOTHrZ5mXkm_UP 6, 33 -- YXfBxGcjglFZqA7gfOyx1AXdJykFDca65jdd4CrDmZDFTovMjdcLVE22lFfd 6, 33 -- xSw3YnYT8tKOHOUQKJthPB4XYcvCnsO9PE_vAgwMEJLxYBrpvCtvwegj55qK 6, 33 -- NG.jxTxr5fPv8KcGijt4M5dIVntw6OeafGuDkQrFks6_WT0LNAxWxRYhoph0 6, 33 -- JO48mwxTG59lD7FIaE_TIBbK4qbfop6G8VM78ycfjWw2QPGDbFLtwjtGjqLa 6, 33 -- DFzn5xckp6bSlAtqBvuxxKosJUDT3_8bVm9ELlZw2klKoJVj71PIFhQFRppJ 6, 33 -- SUuBvYRwa2SjVsdBOwWTN.RKL5TA0rmv6Iks9HptwvnKIN96TAXzH8GN.5eK 6, 33 -- DE0Ep8qnQNrqECq8cRARe_6DtFUKiMJ6OpgAUm9hmYprqdq1M90MEH08XKVt 6, 33 -- BImRzkSM_Ee_IFc9EiyRXVU3rhdl5C4PTuLMqJOo7twNOlNViMw-- 6, 33 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 33 -- Content-Type: text/plain; charset=utf-8 6, 33 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 6, 33 -- Subject: Re: [Microscopy] How to make holey carbon support film myself? 6, 33 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 33 -- In-Reply-To: {201509230940.t8N9eiW8012609-at-ns.microscopy.com} 6, 33 -- Date: Wed, 23 Sep 2015 14:54:30 -0700 6, 33 -- Message-Id: {EE9873D7-A405-412A-BD1F-C83BDEEA4073-at-sbcglobal.net} 6, 33 -- References: {201509230940.t8N9eiW8012609-at-ns.microscopy.com} 6, 33 -- To: Z.Zhou-at-lboro.ac.uk, microscopy-at-microscopy.com 6, 33 -- X-Mailer: Apple Mail (2.2104) 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8NLsZ2W011292 ==============================End of - Headers==============================
Years ago, back when dinosaurs still roamed the earth, I made the holey Formvar films for our lab. We didn't use carbon films back then in the biological microscopy lab. I used the method described by
I used the method described by M A Hayat, Principles and Techniques of Electron Microscopy (Biological Applications, Volume 1), Van Nostrand Reinhold, 1970, p324-332. The method for production of Parlodian (nitrocellulose) or Formvar (polyvinyl formal) plastic films is straightforward but requires practice to learn the technique. The method is the same for production of continuous and holey Parlodian and Formvar films except that glycerol is added to the Parlodian or Formvar solution prior to casting the films; the glycerol is insoluble in the solvents used to dissolve the plastic films. The size of holes in in holey films is proportional to the concentration of glycerol in the mixture: 12% (vol/vol) glycerol gives holes with a maximum diameter of approximately 25 um whereas 0.08% glycerol gives a maximum hole size of 4 um.
Although you can learn the method of holey carbon film preparation, if you don't do it often the process becomes very laborious and time-consuming. I suggest you consider purchasing your holey carbon film grids from an electron microscopy supply house. That's what I did for the last 25 years.
The Hayat book can be found for $8 - $35 on Amazon.
Regarding your question about TEM grid manufacture: metallic TEM grids are electroplated. I don't know how non-metallic grids such as nylon are prepared.
Best regards,
Gary M Brown Polymer Microscopy Consultant
} On Sep 23, 2015, at 2:40 AM, Z.Zhou-at-lboro.ac.uk wrote: } } } Dear Fellow Microscopists, } Has anybody there prepared their own holey carbon film supports? } We usually use the film on Cu mesh grids. How is the mesh grid manufactured? What if I need it on a polymer grid? } How do you control the size of the holes? What is the range of the holes we can achieve? } Very grateful if you can share with me some pros and cons. } Zhaoxia } } Dr Zhaoxia Zhou } Loughborough University, UK } } Dear Zhaoxia, I have done so, and it is trickier than making films without holes. There are several protocols that work, but it may take some time to develop the necessary skills. I found the trickiest part to be the separation of the holey formvar from the glass slide. Commonly, the oil from the skin of oneâs nose is used to coat the slide before dipping in formvar; however, in my case this oil did not workâit did work for former without holes. A solution of Apiazon L in petroleum ether did work for holey formvar. Another difficulty was that separating the film from the slide did not work when the humidity in the lab was high. I will let others answer your other questions. Yours, Bill
==============================Original Headers============================== 6, 33 -- From wtivol-at-sbcglobal.net Wed Sep 23 16:54:37 2015 6, 33 -- Received: from nm9-vm1.access.bullet.mail.bf1.yahoo.com (nm9-vm1.access.bullet.mail.bf1.yahoo.com [216.109.114.192]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8NLsZ2W011292 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 16:54:35 -0500 6, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1443045273; bh=bhrEtY/GmZpAyyyg0/0cqdOV2ZwR6vYXSIbRd8CX2xM=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=Hbk9rE664N4dHQhabzyAr5pOfYZg03ni/2J3W86ZmdLeMwS3UBA0fVnTgH9ROY1BCYSy4aj4QOi5bI4S5rFCZ3PVS6ulunqZTPOitz3z6yqJ8iGG1Xxdf8MV6a5/SHAWD7YTB2x/8k+KU1XS5HGSBSnr2yYqbfeCvvxh5hVsT1KM0AUGt5xWBqXVgQaNEpf0R6Vq46mi805C09iNZsLcNJDWTAEsqlqznTwVXhAltpNR39nHED3mZO5fh72Tu2jyzOq5sRXxtl0dTNsej3Mdf3Aw6bX/WrPRpA8gKhldVMkMH3tkMd7Se2vy26MqvXhy9uNYrCc39QhKrT/vscObVQ== 6, 33 -- Received: from [66.196.81.162] by nm9.access.bullet.mail.bf1.yahoo.com with NNFMP; 23 Sep 2015 21:54:33 -0000 6, 33 -- Received: from [98.139.244.52] by tm8.access.bullet.mail.bf1.yahoo.com with NNFMP; 23 Sep 2015 21:54:33 -0000 6, 33 -- Received: from [127.0.0.1] by smtp114.sbc.mail.bf1.yahoo.com with NNFMP; 23 Sep 2015 21:54:33 -0000 6, 33 -- X-Yahoo-Newman-Id: 255371.97997.bm-at-smtp114.sbc.mail.bf1.yahoo.com 6, 33 -- X-Yahoo-Newman-Property: ymail-3 6, 33 -- X-YMail-OSG: rYAS9U8VM1l.kFXuUZ3ebJuIxWFlnN34vpPCJAI1hngvlAQ 6, 33 -- bGfNwF8SheKl5A4dj7549Q2zLdmg6k._fMex9uwiOTANNLmOTHrZ5mXkm_UP 6, 33 -- YXfBxGcjglFZqA7gfOyx1AXdJykFDca65jdd4CrDmZDFTovMjdcLVE22lFfd 6, 33 -- xSw3YnYT8tKOHOUQKJthPB4XYcvCnsO9PE_vAgwMEJLxYBrpvCtvwegj55qK 6, 33 -- NG.jxTxr5fPv8KcGijt4M5dIVntw6OeafGuDkQrFks6_WT0LNAxWxRYhoph0 6, 33 -- JO48mwxTG59lD7FIaE_TIBbK4qbfop6G8VM78ycfjWw2QPGDbFLtwjtGjqLa 6, 33 -- DFzn5xckp6bSlAtqBvuxxKosJUDT3_8bVm9ELlZw2klKoJVj71PIFhQFRppJ 6, 33 -- SUuBvYRwa2SjVsdBOwWTN.RKL5TA0rmv6Iks9HptwvnKIN96TAXzH8GN.5eK 6, 33 -- DE0Ep8qnQNrqECq8cRARe_6DtFUKiMJ6OpgAUm9hmYprqdq1M90MEH08XKVt 6, 33 -- BImRzkSM_Ee_IFc9EiyRXVU3rhdl5C4PTuLMqJOo7twNOlNViMw-- 6, 33 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 33 -- Content-Type: text/plain; charset=utf-8 6, 33 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 6, 33 -- Subject: Re: [Microscopy] How to make holey carbon support film myself? 6, 33 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 33 -- In-Reply-To: {201509230940.t8N9eiW8012609-at-ns.microscopy.com} 6, 33 -- Date: Wed, 23 Sep 2015 14:54:30 -0700 6, 33 -- Message-Id: {EE9873D7-A405-412A-BD1F-C83BDEEA4073-at-sbcglobal.net} 6, 33 -- References: {201509230940.t8N9eiW8012609-at-ns.microscopy.com} 6, 33 -- To: Z.Zhou-at-lboro.ac.uk, microscopy-at-microscopy.com 6, 33 -- X-Mailer: Apple Mail (2.2104) 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8NLsZ2W011292 ==============================End of - Headers==============================
--
"An expert is a person who has made all the mistakes which can be made in a very narrow field.â and "Prediction is very difficult, especially if it's about the future." Niels Bohr
==============================Original Headers============================== 26, 31 -- From microscopy.gmb-at-gmail.com Wed Sep 23 20:09:09 2015 26, 31 -- Received: from mail-ig0-f177.google.com (mail-ig0-f177.google.com [209.85.213.177]) 26, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8O199sP023994 26, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 20:09:09 -0500 26, 31 -- Received: by igcpb10 with SMTP id pb10so4640317igc.1 26, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 18:09:07 -0700 (PDT) 26, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 26, 31 -- d=gmail.com; s=20120113; 26, 31 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 26, 31 -- :content-type:content-transfer-encoding; 26, 31 -- bh=uY0VK6WhyyvqZsGxzVH81hgilc37SN5n1wN/nEplqfE=; 26, 31 -- b=J9iGaJU7AsqNQ4gCHpeavNSzicUZoajWPXQ/Tm/eAiVnLnDbmkuiMB5axp3JT4uEA7 26, 31 -- rnAR3BSilXuoCCYeqsWiaYfgelfrS5dhbww94Z003+2rqV/daiqRNAzrdnJFRAhVKstQ 26, 31 -- QYBT+K1cR9xPxBYDhbZV+e7+2g7Ej1Na60i5m8MrCdJd9Do073DOkbG/GvPwBHKvfIs+ 26, 31 -- gwOCTcm6x9z5y186VA4N33D6WvGUBfgPD4I5jmin9F8viCbcpx2DmwN1U84P4IO/Xgsj 26, 31 -- bHhVBWXv/T9FVgIcRMvHcPJiztv4q2kWa8qJiHMnojJ1BwxWv5NV3u+M9yHur+HqjylO 26, 31 -- PdtA== 26, 31 -- MIME-Version: 1.0 26, 31 -- X-Received: by 10.50.77.116 with SMTP id r20mr26169213igw.39.1443056947280; 26, 31 -- Wed, 23 Sep 2015 18:09:07 -0700 (PDT) 26, 31 -- Received: by 10.107.176.15 with HTTP; Wed, 23 Sep 2015 18:09:07 -0700 (PDT) 26, 31 -- In-Reply-To: {201509232208.t8NM8xER011198-at-ns.microscopy.com} 26, 31 -- References: {201509232208.t8NM8xER011198-at-ns.microscopy.com} 26, 31 -- Date: Wed, 23 Sep 2015 20:09:07 -0500 26, 31 -- Message-ID: {CADhGOTJMy8GG-wmVdTxFXAakNzCu93zCdmgu50YT2QP3=zFYhw-at-mail.gmail.com} 26, 31 -- Subject: Fwd: [Microscopy] Re: How to make holey carbon support film myself? 26, 31 -- From: Gary Brown {microscopy.gmb-at-gmail.com} 26, 31 -- To: Z.Zhou-at-lboro.ac.uk, Listserver {Microscopy-at-microscopy.com} 26, 31 -- Content-Type: text/plain; charset=UTF-8 26, 31 -- Content-Transfer-Encoding: 8bit 26, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8O199sP023994 ==============================End of - Headers==============================
Have done this using a turbo vacuum system we used for pumping holders, with a tube connected, covered by a vacuum flange with an electrical feedthrough (used parts of an an old penning gauge for that). Inside I clamped a 12VDC halogen lamp to produce heat. Our mechanical workshop machined a small open aluminium grid box which holds 16 grids. This goes into the tube, pump, ramp up the lamp, wait some hours, vent with clean nitrogen. The small dry turbo system is going to be main cost, the other parts can be cheap, you might also consider putting a UV source in .
Best,
Wim Hagen EMBL Heidelberg
} On Sep 22, 2015, at 7:59 PM, steven.spurgeon-at-pnnl.gov wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello everyone, } } Iâve been trying to develop a procedure to clean our STEM samples prior to insertion into our ARM200CF microscope. In particular, we are trying to perform highly analytical work that involves long EELS / EDS mapping, so we would like to reduce hydrocarbon contamination on the surface of our samples. } } Since most of our specimens are oxide lift outs, they are quite robust to heating and plasma cleaning. Typically after a lift out is completed I will plasma clean for 2-5 minutes prior to insertion; however, someone recently suggested baking our samples for a few hours at 50-100 ÂşC to further reduce contamination. It was also suggested that we place our heater in a bell jar-like setup so that we can pump on the sample during the annealing. } } I am considering building such a setup, but I would like to see if anyone has constructed something like this before. I would very much appreciate any design suggestions or other tips to help us clean up our samples. } } Thank you! } ______________________________________ } Steven R. Spurgeon, Ph.D. } Postdoctoral Research Associate } Fundamental and Computational Sciences Directorate } } Pacific Northwest National Laboratory } 902 Battelle Boulevard } P.O. Box 999 MSIN:K8-87 } Richland, WA 99352 } } Tel: +1-509-371-7123 } steven.spurgeon-at-pnnl.gov } www.stevenspurgeon.com {http://www.stevenspurgeon.com/} } www.pnnl.gov {http://www.pnnl.gov/} } } } ==============================Original Headers============================== } 8, 26 -- From prvs=700570384=steven.spurgeon-at-pnnl.gov Tue Sep 22 12:30:27 2015 } 8, 26 -- Received: from Emailgw01.pnnl.gov (emailgw01.pnnl.gov [192.101.109.61]) } 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8MHURDO032198 } 8, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Sep 2015 12:30:27 -0500 } 8, 26 -- Received: from ex10cashub05.pnnl.gov ([130.20.128.106]) } 8, 26 -- by Emailgw01.pnnl.gov with ESMTP/TLS/AES128-SHA; 22 Sep 2015 10:30:25 -0700 } 8, 26 -- Received: from EX10MBOX04.pnnl.gov ([169.254.4.45]) by EX10CASHUB05.pnnl.gov } 8, 26 -- ([130.20.128.106]) with mapi id 14.03.0248.002; Tue, 22 Sep 2015 10:30:25 } 8, 26 -- -0700 } 8, 26 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} } 8, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 8, 26 -- Subject: Bakeout chamber for cleaning STEM samples } 8, 26 -- Thread-Topic: Bakeout chamber for cleaning STEM samples } 8, 26 -- Thread-Index: AQHQ9Vxd32uS5ZaR90Ce2EEADuLCcw== } 8, 26 -- Date: Tue, 22 Sep 2015 17:30:24 +0000 } 8, 26 -- Message-ID: {0B35EE01-8D64-4F05-8015-7CBF1364A5D9-at-pnnl.gov} } 8, 26 -- Accept-Language: en-US } 8, 26 -- Content-Language: en-US } 8, 26 -- X-MS-Has-Attach: } 8, 26 -- X-MS-TNEF-Correlator: } 8, 26 -- x-originating-ip: [130.20.128.10] } 8, 26 -- Content-Type: text/plain; charset="utf-8" } 8, 26 -- Content-ID: {065A10548035E146B399CD2BC7B5875E-at-pnnl.gov} } 8, 26 -- MIME-Version: 1.0 } 8, 26 -- Content-Transfer-Encoding: 8bit } 8, 26 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t8MHURDO032198 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 33 -- From wim.hagen-at-me.com Wed Sep 23 21:04:05 2015 7, 33 -- Received: from pv33p04im-asmtp002.me.com (pv33p04im-asmtp002.me.com [17.143.181.11]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8O245vN012463 7, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Sep 2015 21:04:05 -0500 7, 33 -- Received: from [192.168.1.100] 7, 33 -- (ppp-188-174-3-185.dynamic.mnet-online.de [188.174.3.185]) 7, 33 -- by pv33p04im-asmtp002.me.com 7, 33 -- (Oracle Communications Messaging Server 7.0.5.35.0 64bit (built Mar 31 2015)) 7, 33 -- with ESMTPSA id {0NV5007GESEIM500-at-pv33p04im-asmtp002.me.com} for 7, 33 -- Microscopy-at-microscopy.com; Thu, 24 Sep 2015 02:03:58 +0000 (GMT) 7, 33 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, 7, 33 -- definitions=2015-09-24_01:,, signatures=0 7, 33 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 7, 33 -- kscore.is_bulkscore=2.94209101525667e-15 compositescore=0.684355233276566 7, 33 -- phishscore=0 kscore.is_spamscore=0 rbsscore=0.684355233276566 7, 33 -- recipient_to_sender_totalscore=0 spamscore=0 urlsuspectscore=0.684355233276566 7, 33 -- adultscore=0 kscore.compositescore=0 circleOfTrustscore=0 suspectscore=4 7, 33 -- recipient_domain_to_sender_totalscore=0 bulkscore=0 7, 33 -- recipient_domain_to_sender_domain_totalscore=0 7, 33 -- recipient_to_sender_domain_totalscore=0 classifier=spam adjust=0 reason=mlx 7, 33 -- scancount=1 engine=8.0.1-1412110000 definitions=main-1509240036 7, 33 -- Content-type: text/plain; charset=utf-8 7, 33 -- MIME-version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 7, 33 -- Subject: Re: [Microscopy] Bakeout chamber for cleaning STEM samples 7, 33 -- From: Wim Hagen {wim.hagen-at-me.com} 7, 33 -- In-reply-to: {201509221759.t8MHxcNZ015948-at-ns.microscopy.com} 7, 33 -- Date: Thu, 24 Sep 2015 04:03:53 +0200 7, 33 -- Message-id: {A97B7D7F-BB1E-44F1-925F-962D0528F571-at-me.com} 7, 33 -- References: {201509221759.t8MHxcNZ015948-at-ns.microscopy.com} 7, 33 -- To: Microscopy-at-microscopy.com 7, 33 -- X-Mailer: Apple Mail (2.2104) 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8O245vN012463 ==============================End of - Headers==============================
HI, I wanted to build a similar system for drying of NMP from my TEM grids. not sure if it will work for you, i just hooked up a peltier cooler/heater with an old desktop pc power supply and controlled it with a mechenical relay, which in turn was controlled by Arduino Uno. Temp. feedback was provided by LM-35 sensor which i got in Arduino started kit. Peltier heater and sensor was kept in a vacuum oven which had few holes drilled to make way for wires then it was sealed with epoxy reisin. It was the cheapest and quickest way to do it and it can easily maintain temp of 60 degrees +- 2 degrees. for higher temperatures and better control you can try better heating coils, thermocoulples and PID algorithm rest of setup shall remain same.
On Tue, Sep 22, 2015 at 11:30 PM, {steven.spurgeon-at-pnnl.gov} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello everyone, } } Iâve been trying to develop a procedure to clean our STEM samples prior to insertion into our ARM200CF microscope. In particular, we are trying to perform highly analytical work that involves long EELS / EDS mapping, so we would like to reduce hydrocarbon contamination on the surface of our samples. } } Since most of our specimens are oxide lift outs, they are quite robust to heating and plasma cleaning. Typically after a lift out is completed I will plasma clean for 2-5 minutes prior to insertion; however, someone recently suggested baking our samples for a few hours at 50-100 ÂşC to further reduce contamination. It was also suggested that we place our heater in a bell jar-like setup so that we can pump on the sample during the annealing. } } I am considering building such a setup, but I would like to see if anyone has constructed something like this before. I would very much appreciate any design suggestions or other tips to help us clean up our samples. } } Thank you! } ______________________________________ } Steven R. Spurgeon, Ph.D. } Postdoctoral Research Associate } Fundamental and Computational Sciences Directorate } } Pacific Northwest National Laboratory } 902 Battelle Boulevard } P.O. Box 999 MSIN:K8-87 } Richland, WA 99352 } } Tel: +1-509-371-7123 } steven.spurgeon-at-pnnl.gov } www.stevenspurgeon.com {http://www.stevenspurgeon.com/} } www.pnnl.gov {http://www.pnnl.gov/} } } } ==============================Original Headers============================== } 8, 26 -- From prvs=700570384=steven.spurgeon-at-pnnl.gov Tue Sep 22 12:30:27 2015 } 8, 26 -- Received: from Emailgw01.pnnl.gov (emailgw01.pnnl.gov [192.101.109.61]) } 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8MHURDO032198 } 8, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Sep 2015 12:30:27 -0500 } 8, 26 -- Received: from ex10cashub05.pnnl.gov ([130.20.128.106]) } 8, 26 -- by Emailgw01.pnnl.gov with ESMTP/TLS/AES128-SHA; 22 Sep 2015 10:30:25 -0700 } 8, 26 -- Received: from EX10MBOX04.pnnl.gov ([169.254.4.45]) by EX10CASHUB05.pnnl.gov } 8, 26 -- ([130.20.128.106]) with mapi id 14.03.0248.002; Tue, 22 Sep 2015 10:30:25 } 8, 26 -- -0700 } 8, 26 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} } 8, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 8, 26 -- Subject: Bakeout chamber for cleaning STEM samples } 8, 26 -- Thread-Topic: Bakeout chamber for cleaning STEM samples } 8, 26 -- Thread-Index: AQHQ9Vxd32uS5ZaR90Ce2EEADuLCcw== } 8, 26 -- Date: Tue, 22 Sep 2015 17:30:24 +0000 } 8, 26 -- Message-ID: {0B35EE01-8D64-4F05-8015-7CBF1364A5D9-at-pnnl.gov} } 8, 26 -- Accept-Language: en-US } 8, 26 -- Content-Language: en-US } 8, 26 -- X-MS-Has-Attach: } 8, 26 -- X-MS-TNEF-Correlator: } 8, 26 -- x-originating-ip: [130.20.128.10] } 8, 26 -- Content-Type: text/plain; charset="utf-8" } 8, 26 -- Content-ID: {065A10548035E146B399CD2BC7B5875E-at-pnnl.gov} } 8, 26 -- MIME-Version: 1.0 } 8, 26 -- Content-Transfer-Encoding: 8bit } 8, 26 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t8MHURDO032198 } ==============================End of - Headers==============================
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Title-Subject: [Filtered] A Protocol for X-Max (Oxford Instruments, INCA) analysis system calibration
Message: Hi Colleagues!
We have SEM Zeiss EVO with microanalysis system X-Max (Oxford Instruments, INCA software) and the etalons for its calibration. But we do not have a protocol for this procedure. We do not have a warranty or money. How can we do the quantitative calibration? Could anyone to send me a protocol of calibration?
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Title-Subject: [Filtered] Position Available - TEM Senior Microscopist - Adelaide Microscopy
Message: Message: The University of Adelaide, in Adelaide, Australia, is seeking a TEM microscopist who will be responsible for the management, operation, training and maintenance of the FEI Titan Themis TEM. A major part of this position is the development of application protocols which allow users to maximise their use of the instrument.
Term of position: This continuing position is available immediately.
Full position description and instructions on how to apply can be found at: http://careers.adelaide.edu.au/cw/en/job/493949/tem-senior-microscopist
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I apologize for taking up listserv bandwidth but I have a problem. I have been trying to contact Zeiss Sales about getting an additional camera and software for one of our Zeiss Axioobserver Z1 light microscopes for the past few weeks. I have tried multiple contacts (LM, EM, sales, service, admin) and methods including the general web site sales contact form, all of which has resulted in no success. So now I am trying the listserv.
At this point I am beginning to wonder if Zeiss is still in business? I get advertizing emails but can´t any actual response from anyone. Does anyone have any information of getting a hold of Zeiss these days?
Secondly, I would be happy to hear from other vendors who can provide: a camera to our Axioobserver Z1 with two side ports. We need to do larger field-of-view montaging. The main interest is "whole slide" scanning. Our Axioobserver Z1 is fully motorized (including stage), with Phase, DIC, and fluorescence. What we are missing is a camera mount\tube, a wide-field color camera and software to run it.
Thank you
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
I don't know about Zeiss North America, but Zeiss has gone back to dealerships for their light microscopes and confocals. The dealer for my area is Lukas Microscope, based in Mundelein IL, so they might cover you as well. lukasmicroscope.com (I got contacted by them this summer for the first time, without hearing anything from our previous Zeiss North Am reps.)
Phil
} Hello one and all, } } I apologize for taking up listserv bandwidth but I have a problem. I have } been trying to contact Zeiss Sales about getting an additional camera and software } for one of our Zeiss Axioobserver Z1 light microscopes for the past few weeks. I } have tried multiple contacts (LM, EM, sales, service, admin) and methods including } the general web site sales contact form, all of which has resulted in no success. So } now I am trying the listserv. } } At this point I am beginning to wonder if Zeiss is still in business? I get } advertizing emails but can´t any actual response from anyone. Does anyone have } any information of getting a hold of Zeiss these days? } } Secondly, I would be happy to hear from other vendors who can provide: a } camera to our Axioobserver Z1 with two side ports. We need to do larger } field-of-view montaging. The main interest is "whole slide" scanning. Our } Axioobserver Z1 is fully motorized (including stage), with Phase, DIC, and } fluorescence. What we are missing is a camera mount\tube, a wide-field color } camera and software to run it. } } Thank you } } } Richard E. Edelmann, Ph.D., Director } Center for Advanced Microscopy& Imaging } 9C Upham Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-miamioh.edu } http://www.cami.muohio.edu -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 35 -- From oshel1pe-at-cmich.edu Thu Sep 24 08:43:16 2015 4, 35 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8ODhG3n024635 4, 35 -- for {microscopy-at-microscopy.com} ; Thu, 24 Sep 2015 08:43:16 -0500 4, 35 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 4, 35 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8ODhDxZ024511; 4, 35 -- Thu, 24 Sep 2015 09:43:13 -0400 4, 35 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 4, 35 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 4, 35 -- (TLS) id 14.3.248.2; Thu, 24 Sep 2015 09:43:12 -0400 4, 35 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 4, 35 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 4, 35 -- (TLS) id 14.3.248.2; Thu, 24 Sep 2015 09:43:12 -0400 4, 35 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 35 -- cas2.central.cmich.local (141.209.15.141) with Microsoft SMTP Server (TLS) id 4, 35 -- 14.3.248.2; Thu, 24 Sep 2015 09:43:11 -0400 4, 35 -- Message-ID: {5603FDF0.2060000-at-cmich.edu} 4, 35 -- Date: Thu, 24 Sep 2015 09:43:12 -0400 4, 35 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 35 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 35 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 35 -- MIME-Version: 1.0 4, 35 -- To: {Edelmare-at-miamioh.edu} , micro {microscopy-at-microscopy.com} 4, 35 -- Subject: Re: [Microscopy] Has Zeiss North America gone belly up? 4, 35 -- References: {201509241337.t8ODbFpn020659-at-ns.microscopy.com} 4, 35 -- In-Reply-To: {201509241337.t8ODbFpn020659-at-ns.microscopy.com} 4, 35 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-Originating-IP: [141.209.2.100] 4, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 35 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 35 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 4, 35 -- X-CanItPRO-Stream: default 4, 35 -- X-Canit-Stats-ID: 02Pl1HdGV - 659e0cffa4eb - 20150924 4, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
You might try contacting McCrone Associates and checking with them about your camera needs and about the status of Zeiss. I am not aware of them having shut down but I am certain that McCrone Associates would know. You can get their contact information from putting their name into a search engine with the work "microscopy" and get their number from there. Again, they may be able to help you with the camera too. I know that they sell Olympus and Nikon instruments but am not sure about Zeiss. Regardless, given their status I think they would be able to tell you what the scoop is.
Sincerely,
James Neal-Kababick Director Flora Research Laboratories, LLC An FDA and DEA Registered & Inspected Laboratory Fellow AOAC International President Elect, PSW-AOAC Vice Chair USP {2251} ADSDDA Expert Panel United States Pharmacopeia NBDS Expert Committee USP Joint Standards Setting Subcommittee for Reference Standards (JS3) Adjunct Faculty Bastyr University Botanical Medicine Department 1-541-472-0980 phone jimk-at-floraresearch.com www.floraresearch.com
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-----Original Message----- X-from: Edelmare-at-miamioh.edu [mailto:Edelmare-at-miamioh.edu] Sent: Thursday, September 24, 2015 6:38 AM To: James Neal-Kababick {jimk-at-floraresearch.com}
Hello one and all,
I apologize for taking up listserv bandwidth but I have a problem. I have been trying to contact Zeiss Sales about getting an additional camera and software for one of our Zeiss Axioobserver Z1 light microscopes for the past few weeks. I have tried multiple contacts (LM, EM, sales, service, admin) and methods including the general web site sales contact form, all of which has resulted in no success. So now I am trying the listserv.
At this point I am beginning to wonder if Zeiss is still in business? I get advertizing emails but can´t any actual response from anyone. Does anyone have any information of getting a hold of Zeiss these days?
Secondly, I would be happy to hear from other vendors who can provide: a camera to our Axioobserver Z1 with two side ports. We need to do larger field-of-view montaging. The main interest is "whole slide" scanning. Our Axioobserver Z1 is fully motorized (including stage), with Phase, DIC, and fluorescence. What we are missing is a camera mount\tube, a wide-field color camera and software to run it.
Thank you
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
==============================Original Headers============================== 15, 27 -- From Edelmare-at-miamioh.edu Thu Sep 24 08:24:19 2015 15, 27 -- Received: from mualmaip02.mcs.muohio.edu (mualmaip02.mcs.muohio.edu [134.53.6.8]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8ODOJxr003897 15, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Sep 2015 08:24:19 -0500 15, 27 -- X-DNSBL-MILTER: Skipped 15, 27 -- Received: from [10.34.160.232] ([10.34.160.232]) 15, 27 -- by mualmaip02.mcs.muohio.edu (8.14.4/8.14.4) with ESMTP id t8ODNjN1024060 15, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Sep 2015 09:23:46 -0400 15, 27 -- From: "Richard E. Edelmann" {Edelmare-at-miamioh.edu} 15, 27 -- To: Microscopy-at-microscopy.com 15, 27 -- Date: Thu, 24 Sep 2015 09:23:20 -0400 15, 27 -- MIME-Version: 1.0 15, 27 -- Subject: Has Zeiss North America gone belly up? 15, 27 -- Message-ID: {5603F948.415.1EE099C1-at-Edelmare.miamioh.edu} 15, 27 -- Priority: normal 15, 27 -- X-mailer: Pegasus Mail for Windows (4.70) 15, 27 -- Content-type: text/plain; charset=ISO-8859-1 15, 27 -- Content-description: Mail message body 15, 27 -- X-Antivirus: avast! (VPS 150923-1, 09/23/2015), Outbound message 15, 27 -- X-Antivirus-Status: Clean 15, 27 -- X-Miami-MailScanner-Information: Please contact the ISP for more information 15, 27 -- X-Miami-MailScanner-ID: t8ODNjN1024060 15, 27 -- X-Miami-MailScanner: Found to be clean 15, 27 -- X-Miami-MailScanner-From: edelmare-at-miamioh.edu 15, 27 -- X-Spam-Status: No 15, 27 -- Content-Transfer-Encoding: 8bit 15, 27 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id t8ODOJxr003897 ==============================End of - Headers==============================
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I'm assuming this is from an educator. I've already suggested the Project MICRO website, as well as on-line pages like microscopyu.com and the molecular expressions microscopy pages, and suggested some playing with resolution, empty magnification, and what distance does each pixel on their phone's camera represent when they take a photo of e.g. a mm ruler. As well as "how big is a human hair?", given that's a standard measure in the popular press. And doing measurements and stats to determine that. But how about some other ideas?
Phil
} Ask a Microscopist } } } The following Ask a Microscopist form submission was received from your website. } } } Name: Timmon Hogan } } School: Elko Middle School } } Grade/Education Level: Middle School } } Location: Richmond, VA US } } Email: hogantimmon-at-gmail.com } } Subject: analysis } } Your Question: What formulas would a 8th grader need to learn more about to practice math involved in microscopy ? }
==============================Original Headers============================== 6, 32 -- From oshel1pe-at-cmich.edu Thu Sep 24 15:51:34 2015 6, 32 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8OKpYuX016955 6, 32 -- for {microscopy-at-microscopy.com} ; Thu, 24 Sep 2015 15:51:34 -0500 6, 32 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 6, 32 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t8OKpWj2001833 6, 32 -- for {microscopy-at-microscopy.com} ; Thu, 24 Sep 2015 16:51:32 -0400 6, 32 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 6, 32 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 6, 32 -- (TLS) id 14.3.248.2; Thu, 24 Sep 2015 16:51:32 -0400 6, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 6, 32 -- cas2.central.cmich.local (141.209.15.141) with Microsoft SMTP Server (TLS) id 6, 32 -- 14.3.248.2; Thu, 24 Sep 2015 16:51:31 -0400 6, 32 -- Message-ID: {56046253.8010109-at-cmich.edu} 6, 32 -- Date: Thu, 24 Sep 2015 16:51:31 -0400 6, 32 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 6, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 32 -- MIME-Version: 1.0 6, 32 -- To: micro {microscopy-at-microscopy.com} 6, 32 -- Subject: Re: Ask a Microscopist - what math for 8th graders doing microscopy? 6, 32 -- References: {65896995.3643.1443122076830.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 6, 32 -- In-Reply-To: {65896995.3643.1443122076830.JavaMail.EWHSERVER1858$-at-ewhserver1139.edgewebhosting.net} 6, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 32 -- Content-Transfer-Encoding: 7bit 6, 32 -- X-Originating-IP: [141.209.2.100] 6, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 6, 32 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 6, 32 -- X-CanItPRO-Stream: default 6, 32 -- X-Canit-Stats-ID: 05Pl8Pw6r - 2f5a2c367ab1 - 20150924 6, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Well done, Ben. These are such valuable lessons to learn. Your movie was especially effective.
When we are on the road, we often use a business card for this demonstration, placed perpendicular to the stage. Also, I have a piece of screwdriver handle stock (about 1" in diameter), polished on one end, placed on a slide with a drop of oil under it. In the old days, we used to use Uraniium glass. I understand that Jerry Sedgewick (jerry-at-imagingandanalysis.com) has a new supplier, so that approach may also come back into vogue.
As for "poor man's oblique": Unless one has a condenser with an Abbe slider, I doubt that any of us could have done better. No apologies necessary! So here's question to put to your students: What impact does off-setting the zero order have on resolution?
Good hunting! Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education ... "Education, not Training" 7101 Royal Glen Trail, Suite A - McKinney, TX 75070 - P: 972-924-5310 www.MicroscopyEducation.com
NEW! Getting involved in Raman or FTIR? MME is now offering courses in these areas specifically for microscopists! Now scheduling courses through the end of 2015. We can customize a course on nearly any topic, from fluorescence to confocal to image analysis to SEM/TEM. Call today for a free training evaluation.
At 10:31 PM 9/23/2015, benjamin.smith-at-ou.edu wrote:
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==============================Original Headers============================== 11, 30 -- From bfoster-at-the-mip.com Thu Sep 24 16:34:21 2015 11, 30 -- Received: from barcelona.directrouter.com (barcelona.directrouter.com [72.249.98.64]) 11, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8OLYKih006379 11, 30 -- for {Microscopy-at-Microscopy.com} ; Thu, 24 Sep 2015 16:34:21 -0500 11, 30 -- Message-Id: {201509242134.t8OLYKih006379-at-ns.microscopy.com} 11, 30 -- Received: from 99-168-104-53.lightspeed.rcsntx.sbcglobal.net ([99.168.104.53]:60871 helo=Bs-PC.the-mip.com) 11, 30 -- by barcelona.directrouter.com with esmtpsa (TLSv1:DHE-RSA-AES256-SHA:256) 11, 30 -- (Exim 4.85) 11, 30 -- (envelope-from {bfoster-at-the-mip.com} ) 11, 30 -- id 1ZfE9i-0006bE-2t; Thu, 24 Sep 2015 16:34:18 -0500 11, 30 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 30 -- Date: Thu, 24 Sep 2015 16:34:10 -0500 11, 30 -- To: benjamin.smith-at-ou.edu, 11, 30 -- "MIcroscopy-Microscopy.com" {Microscopy-at-Microscopy.com} 11, 30 -- From: Barbara Foster {bfoster-at-the-mip.com} 11, 30 -- Subject: Re: [Microscopy] LM Demonstrating the field and aperture 11, 30 -- diaphragm as well as 11, 30 -- In-Reply-To: {201509232203.t8NM3gY3031779-at-ns.microscopy.com} 11, 30 -- References: {201509232203.t8NM3gY3031779-at-ns.microscopy.com} 11, 30 -- Mime-Version: 1.0 11, 30 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 11, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 11, 30 -- X-AntiAbuse: Primary Hostname - barcelona.directrouter.com 11, 30 -- X-AntiAbuse: Original Domain - microscopy.com 11, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 11, 30 -- X-AntiAbuse: Sender Address Domain - the-mip.com 11, 30 -- X-Get-Message-Sender-Via: barcelona.directrouter.com: authenticated_id: bfoster-at-the-mip.com 11, 30 -- X-Source: 11, 30 -- X-Source-Args: 11, 30 -- X-Source-Dir: ==============================End of - Headers==============================
I spent considerable time with the Zeiss folks at M&M early last month and found them to be very much alive and very responsive to customers, so was surprised to hear about your problems. I've forwarded your note to them, with the hope that they will get in touch with you soon.
Hope this was helpful,
Best regards, Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education ... "Education, not Training" 7101 Royal Glen Trail, Suite A - McKinney, TX 75070 - P: 972-924-5310 www.MicroscopyEducation.com
NEW! Getting involved in Raman or FTIR? MME is now offering courses in these areas specifically for microscopists! Now scheduling courses through the end of 2015. We can customize a course on nearly any topic, from fluorescence to confocal to image analysis to SEM/TEM. Call today for a free training evaluation.
At 12:20 PM 9/24/2015, Edelmare-at-miamioh.edu wrote:
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I think the concept of optical resolution, instead of magnification, is important to any imaging device from a cell phone to TEM.
r = 1.22 lambda/2nsin theta = 0.61 x lambda/NA
where r = minimum distance between resolvable points lambda = wavelength of light n = refractive index of the media theta = the half angle of the pencil of light that enters the objective NA = the numerical aperture
Here, the concept of numerical aperture or light gathering capability of the lens is a important and easy for most middle-school kids to understand if properly taught. It doesn't take long before one can understand the important of NA when buying binoculars, and certainly using a microscope.
The concepts of resolution and empty magnification are key. Using Photoshop, I have enlarged portions of images of different resolution to illustrate the effect of spatial resolution on image quality, then going on to explain how to optimize resolution and contrast.
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
I'm assuming this is from an educator. I've already suggested the Project MICRO website, as well as on-line pages like microscopyu.com and the molecular expressions microscopy pages, and suggested some playing with resolution, empty magnification, and what distance does each pixel on their phone's camera represent when they take a photo of e.g. a mm ruler. As well as "how big is a human hair?", given that's a standard measure in the popular press. And doing measurements and stats to determine that. But how about some other ideas?
Phil
} Ask a Microscopist } } } The following Ask a Microscopist form submission was received from your website. } } } Name: Timmon Hogan } } School: Elko Middle School } } Grade/Education Level: Middle School } } Location: Richmond, VA US } } Email: hogantimmon-at-gmail.com } } Subject: analysis } } Your Question: What formulas would a 8th grader need to learn more about to practice math involved in microscopy ? }
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Title-Subject: [Filtered] EM Lab manager position available, Naval Research Lab, Washington DC
Message: If we say, "I can name that atom in 10 seconds," do you say, "I bet I can do it in 5,"?
The NRL Nanoscale Materials Section in the Materials Science and Technology Division of the Naval Research Lab seeks an electron microscopy lab manager who will take pride in ensuring world-class, DoD-leading performance of section microscopes to enable next generation materials development. Primary responsibilities will be to assist in on-going research projects, and day-to-day maintenance of and user training on a Nion UltraSTEM 200 with single-atom-sensitivity EDS, and a JEOL 2200FS FEG S/TEM with in situ electrical, and electrochemical cell holders. Secondary responsibilities will include technique development for sample preparation, analysis and patterning with a fully-accessorized FEI Helios G3 focused ion beam microscope in the NRL Institute for Nanoscience. Necessary qualifications include US citizenship,and a Ph.D. in physics, materials science or related field, or bachelors degree and a minimum of 5 years work experience in transmission electron microscopy in a research environment. Skill in the operation of an aberration-corrected STEM is required; experience in UHV STEM operation preferred. Demonstrated experience in automation through scripting/coding on at least one platform is also required, e.g., Digital Micrograph, MatLab, JEM Tool Box, Python or Avizo.
The Naval Research Lab is a civilian-staffed, federally-funded research lab with a mission to promote national security through world-leading basic scientific research. The Nanoscale Materials Section conducts cross-disciplinary, collaborative studies of nanomaterials ranging from DNA origami to core-shell quantum dots to interstellar dust.
Questions regarding the position and CV's from potential applicants can be emailed to: rhonda.stroud-at-nrl.navy.mil
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Title-Subject: [Filtered] Suggestions for books to be reviewed for Microscopy and Microanalysis journal
Message: Hello,
I am the Book Review Editor for the journal Microscopy and Microanalysis. I am looking for recommendations of books that that were recently published or will be published in the near future that would be of interest to our readership. If accepted, we would find someone to review these books. Please reply to me offline at cgoldsmith-at-cdc.gov.
Thank you for your help.
Cynthia
Cynthia S. Goldsmith, MGS Book Review Editor, Microscopy and Microanalysis
Infectious Diseases Pathology Branch Centers for Disease Control and Prevention (CDC) Atlanta, GA 30329
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Email: cbuser125-at-gmail.com Name: Chris
Title-Subject: [Filtered] JEOL 1400 TEM stage problem
Message: Hi everyone,
I have a weird problem with a JEOL 1400 IÂm using, which is not under service contract. The stage can only be moved toward the center on both axes using the trackball or the XY buttons. This works from any sector, meaning the stage trackball / buttons / motors are able to move in all directions, but only toward the center. To come to a position farther to the edge, you have to shoot the stage over diagonally to the opposing quadrant, and then shoot back to the original quadrant on a position farther out. You canÂt shoot away from the middle in the same quadrant either. So itÂs like you have to Âreset the stage by crossing to the other side with the fast Âshoot function. IÂve also been told that it used to work again after a power failure and then went back to not working after a week or so.
Does anyone have an idea where the problem could be and how to trouble shoot it?
Thanks, Chris
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Email: cbuser125-at-gmail.com Name: Chris
Title-Subject: [Filtered] JEOL 1400 TEM stage problem
Message: Hi everyone,
I have a weird problem with a JEOL 1400 IÂm using, which is not under service contract. The stage can only be moved toward the center on both axes using the trackball or the XY buttons. This works from any sector, meaning the stage trackball / buttons / motors are able to move in all directions, but only toward the center. To come to a position farther to the edge, you have to shoot the stage over diagonally to the opposing quadrant, and then shoot back to the original quadrant on a position farther out. You canÂt shoot away from the middle in the same quadrant either. So itÂs like you have to Âreset the stage by crossing to the other side with the fast Âshoot function. IÂve also been told that it used to work again after a power failure and then went back to not working after a week or so.
Does anyone have an idea where the problem could be and how to trouble shoot it?
Thanks, Chris
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Email: chris.guerin-at-irc.vib-ugent.be Name: Chris Guerin
Organization: VIB Bio Imaging Core
Title-Subject: [Filtered] Bio IT Image specialist position
Message: Hi Everyone:
There is an opening for an Bio IT image specialist in Ghent Belgium. The details are as follows
VIB is looking to expand its Institute-wide bioinformatics platform, BITS, with a specialist in biological imaging IT to provide image analyses and setup of new computing capabilities in advanced microscopy. We are looking for a dedicated individual to join the BITS team who will jointly work together in projects for the VIB Bio Imaging Core in Ghent and Leuven (https://corefacilities.vib.be/bic) as well as the Discovery Sciences platform in Leuven.
Ideally the candidate has outstanding communication skills to establish a close working relationship with researchers and with staff of the imaging core facilities. The goal of this partnership is to make sure that imaging experiments are planned, designed and performed in a way that the data produced is suitable for subsequent image processing and image analysis. BITS will also interact with computer vision scientists in other research groups working on specialized image analysis problems. This close relationship will allow the integration of cutting-edge image analysis solutions into the service portfolio.
Responsibilities include:
Help scientists rapidly design and build successful image analysis pipelines and data flows using a variety of open-source and commercial software packages as well as writing custom code and tools when necessary. Develop and coordinate new regional and (inter)national collaboration between BITS, the Bio Imaging Core and other field experts (e.g. iMinds). Coordinate and network all the different and currently distributed image analysis resources of the cores. Assist in training students and staff in image analysis and allowable image manipulation methods. Provide an interface between BITS, the departmental ITs, and the Bio Imaging Core. Assist the cores in data organization, analysis and archiving. Develop a method of data consolidation where essential data is safeguarded, and non-essential or duplicative data is not taking up valuable archive space. Develop an image data mining resource for VIB and beyond. Organize, administer and help promote a VIB repository of data that can be accessed by different VIB research groups and for additional use by other academic centers. Collaborate with the researchers to develop and implement algorithms that solve particular image processing problems from cell and developmental biology.
The candidate will split his/her time between the Ghent, Leuven core facilities and Discovery Sciences and may also be required to travel to other VIB sites in Brussels and Antwerp. More information on the BITS and the VIB Bio Imaging Cores can be found at https://www.bits.vib.be and https://corefacilities.vib.be/bic Profile
The candidate must have an advanced academic degree or equivalent experience and the following skills:
Extensive background in image analysis, algorithm development and statistical analysis of image-based data. Knowledge of microscopy and tissue/cell morphology would be an advantage but is not essential. Knowledge of Image J/FIJI, Imaris, Volocity, IMOD, Amira, Ilastik or Reconstruct would be an advantage.
All applications must be through the VIB Jobs webpages: http://www.vib.be/en/jobs/Pages/Biological-Image-IT-Specialist.aspx.
Informal enquiries can be sent to chris.guerin-at-irc.vib-ugent.be
Best,
Chris
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I wish to thank one and all for comments and contacts with my our issue of getting a hold of Zeiss. Zeiss has contacted me and hopefully we can continue to improve communications here in the Midwest. I received some honestly concerned responses from Zeiss reps in the Northeast, Texas, and Arizona and I thank you.
The responses show and remind us we are a true community of both users and vendors, and our community is at its best when we remember that, and we work together. We each have our own priorities but should not be incompatible and should never divide us. As we have seen on this listserv in the past an "Us vs Them" attitude is not helpful. Like a true community we do need to discuss and share events and issues. Not just at the once a year block party that is the M&M meeting, but in open discussions on this listserv.
Both users and vendors always need to remember that we are partners, and if it wasn´t for the other, neither of us would be here.
Have a great weekend.
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: Edelmare-at-miamioh.edu [mailto:Edelmare-at-miamioh.edu] Sent: Friday, September 25, 2015 8:51 AM To: Dusevich, Vladimir
I wish to thank one and all for comments and contacts with my our issue of getting a hold of Zeiss. Zeiss has contacted me and hopefully we can continue to improve communications here in the Midwest. I received some honestly concerned responses from Zeiss reps in the Northeast, Texas, and Arizona and I thank you.
The responses show and remind us we are a true community of both users and vendors, and our community is at its best when we remember that, and we work together. We each have our own priorities but should not be incompatible and should never divide us. As we have seen on this listserv in the past an "Us vs Them" attitude is not helpful. Like a true community we do need to discuss and share events and issues. Not just at the once a year block party that is the M&M meeting, but in open discussions on this listserv.
Both users and vendors always need to remember that we are partners, and if it wasn´t for the other, neither of us would be here.
Have a great weekend.
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
Hello, everyone. I am attempting Correlative Light and Electron Microscopy (CLEM) for the first time. I am having trouble making tissue samples using 0.2% UA and embedding in Lowicryl. When i section, the tissue is getting crumbled and sections are not stable on the grids. After imaging the grids on the LM, the sections fall off from the grids. Therefore i can't not perform traditional poststaining with UA and LC.
Please help me if you have any tips on CLEM procedures for tissue samples
Thank you
Sincerely,
Jo
==============================Original Headers============================== 7, 45 -- From Kyoung.Jo-at-rockets.utoledo.edu Fri Sep 25 10:50:23 2015 7, 45 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2on0077.outbound.protection.outlook.com [65.55.169.77]) 7, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8PFoMNJ014382 7, 45 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Sep 2015 10:50:23 -0500 7, 45 -- Received: from BN1PR01MB312.prod.exchangelabs.com (10.242.213.147) by 7, 45 -- BN1PR01MB309.prod.exchangelabs.com (10.242.213.140) with Microsoft SMTP 7, 45 -- Server (TLS) id 15.1.274.16; Fri, 25 Sep 2015 15:50:20 +0000 7, 45 -- Received: from BN1PR01MB312.prod.exchangelabs.com ([169.254.5.215]) by 7, 45 -- BN1PR01MB312.prod.exchangelabs.com ([169.254.5.215]) with mapi id 7, 45 -- 15.01.0274.009; Fri, 25 Sep 2015 15:50:20 +0000 7, 45 -- From: "Jo, Kyoung Ha" {Kyoung.Jo-at-rockets.utoledo.edu} 7, 45 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 45 -- Subject: Does anyone know how to perform CLEM for tissue samples ? 7, 45 -- Thread-Topic: Does anyone know how to perform CLEM for tissue samples ? 7, 45 -- Thread-Index: AQHQ96nha1I2IqjjlkO9P0W0N7jI8A== 7, 45 -- Date: Fri, 25 Sep 2015 15:50:20 +0000 7, 45 -- Message-ID: {BN1PR01MB3127DD82705DC7485CB7D29BF420-at-BN1PR01MB312.prod.exchangelabs.com} 7, 45 -- Accept-Language: en-US 7, 45 -- Content-Language: en-US 7, 45 -- X-MS-Has-Attach: 7, 45 -- X-MS-TNEF-Correlator: 7, 45 -- authentication-results: spf=none (sender IP is ) 7, 45 -- smtp.mailfrom=Kyoung.Jo-at-rockets.utoledo.edu; 7, 45 -- x-originating-ip: [131.183.181.102] 7, 45 -- x-microsoft-exchange-diagnostics: 1;BN1PR01MB309;5:17Lg9OHvF3qR9wkqGteOYF4hUONt/th32YpnYPOFxwm+NKE9Q4DYA86Q5z1XhYLl78eyZTen7QpcTF7wgGvslgmQiY2dHmvHssC6NhugAB6tu4BUe3CWQRCUC1cHdBbP33XYXGATZInMjLTWAjdrAA==;24:Ema42JzX86AgbBDwV1wwhKKkRVPOQyjcDTXPfxN2cr8u/VLv88Ckph0NpuXQIqteJD2MdqaqQfvooL8Gy5JhqlIjMWYODAzWOPe0DNQ6nBY=;20:EkNEkpONzRxEls4O4kVNhUTsT3veheY47UMHVT5GO08C80PsJRQ0Dr86yHuKLN/Jn/IIwF0ZGn4uAWz6SUme0g== 7, 45 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BN1PR01MB309; 7, 45 -- x-microsoft-antispam-prvs: {BN1PR01MB309C137F2C19724CE577DDABF420-at-BN1PR01MB309.prod.exchangelabs.com} 7, 45 -- x-exchange-antispam-report-test: UriScan:; 7, 45 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(2401047)(5005006)(520078)(8121501046)(3002001);SRVR:BN1PR01MB309;BCL:0;PCL:0;RULEID:;SRVR:BN1PR01MB309; 7, 45 -- x-forefront-prvs: 07106EF9B9 7, 45 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(199003)(53474002)(189002)(74316001)(5001960100002)(10400500002)(102836002)(62966003)(107886002)(105586002)(64706001)(106356001)(450100001)(110136002)(5002640100001)(5003600100002)(189998001)(68736005)(87936001)(5001830100001)(4001540100001)(97736004)(66066001)(77156002)(2900100001)(81156007)(11100500001)(46102003)(54356999)(40100003)(86362001)(50986999)(5007970100001)(106116001)(2501003)(92566002)(75432002)(88552001)(5004730100002)(89122001)(229853001)(101416001)(5001860100001)(33656002)(2351001)(122556002);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR01MB309;H:BN1PR01MB312.prod.exchangelabs.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 7, 45 -- received-spf: None (protection.outlook.com: rockets.utoledo.edu does not 7, 45 -- designate permitted sender hosts) 7, 45 -- spamdiagnosticoutput: 1:23 7, 45 -- spamdiagnosticmetadata: NSPM 7, 45 -- Content-Type: text/plain; charset="iso-8859-1" 7, 45 -- MIME-Version: 1.0 7, 45 -- X-OriginatorOrg: rockets.utoledo.edu 7, 45 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 25 Sep 2015 15:50:20.1830 7, 45 -- (UTC) 7, 45 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 7, 45 -- X-MS-Exchange-CrossTenant-id: 1d6b1707-baa9-4a3d-a8f8-deabfb3d467b 7, 45 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN1PR01MB309 7, 45 -- Content-Transfer-Encoding: 8bit 7, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8PFoMNJ014382 ==============================End of - Headers==============================
X-from: rhonda.stroud-at-nrl.navy.mil This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rhonda.stroud-at-nrl.navy.mil as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] EM Lab manager position available, Naval Research Lab, Washington DC
Message: If we say, "I can name that atom in 10 seconds," do you say, "I bet I can do it in 5,"?
The NRL Nanoscale Materials Section in the Materials Science and Technology Division of the Naval Research Lab seeks an electron microscopy lab manager who will take pride in ensuring world-class, DoD-leading performance of section microscopes to enable next generation materials development. Primary responsibilities will be to assist in on-going research projects, and day-to-day maintenance of and user training on a Nion UltraSTEM 200 with single-atom-sensitivity EDS, and a JEOL 2200FS FEG S/TEM with in situ electrical, and electrochemical cell holders. Secondary responsibilities will include technique development for sample preparation, analysis and patterning with a fully-accessorized FEI Helios G3 focused ion beam microscope in the NRL Institute for Nanoscience. Necessary qualifications include US citizenship,and a Ph.D. in physics, materials science or related field, or bachelors degree and a minimum of 5 years work experience in transmission electron microscopy in a research environment. Skill in the operation of an aberration-corrected STEM is required; experience in UHV STEM operation preferred. Demonstrated experience in automation through scripting/coding on at least one platform is also required, e.g., Digital Micrograph, MatLab, JEM Tool Box, Python or Avizo.
The Naval Research Lab is a civilian-staffed, federally-funded research lab with a mission to promote national security through world-leading basic scientific research. The Nanoscale Materials Section conducts cross-disciplinary, collaborative studies of nanomaterials ranging from DNA origami to core-shell quantum dots to interstellar dust.
Questions regarding the position and CV's from potential applicants can be emailed to: rhonda.stroud-at-nrl.navy.mil
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Sat Sep 26 09:57:38 2015 14, 40 -- Received: from mail-qk0-f172.google.com (mail-qk0-f172.google.com [209.85.220.172]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8QEvc1o029096 14, 40 -- for {microscopy-at-microscopy.com} ; Sat, 26 Sep 2015 09:57:38 -0500 14, 40 -- Received: by qkdw123 with SMTP id w123so52848956qkd.0 14, 40 -- for {microscopy-at-microscopy.com} ; Sat, 26 Sep 2015 07:57:36 -0700 (PDT) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:reply-to:references:to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=X+JjcdlFyvjpGOkznYExX43lsHOQl0cigrsKCwu1gxM=; 14, 40 -- b=RPW93wGR/4AK5XaCR0fkckv04MEcemHCob5Tme8C/+CTvGT33pPEEytQEVBElSdJO5 14, 40 -- 0TY5J3j8yHrREAtKEEoWfP4MZ/nQzFVA6VcXzZXirSX5OBMmcHaxbgb7GunAOkiFXYJq 14, 40 -- pgS9VFUMAZXPdVNK0dcypUld1/PHDFYbVIdHzjoioERK0A3qzQvTGlCFNxYIHiZaSkr7 14, 40 -- o41qTV55KD7/6lxiKwjcwjOL0ThLtYWX+Tiq/kx/YACqsxqoHeCJ9it1bdIeOnKjBSyV 14, 40 -- P3YZliRs976l7Sfh4CdOo92xka8HyOktjkK8XHdEADxU4L174QlNfLsfaTP+u1mrSW1A 14, 40 -- OiUg== 14, 40 -- X-Received: by 10.55.217.71 with SMTP id u68mr4518241qki.27.1443279456563; 14, 40 -- Sat, 26 Sep 2015 07:57:36 -0700 (PDT) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id 19sm3525685qhr.38.2015.09.26.07.57.35 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 40 -- Sat, 26 Sep 2015 07:57:35 -0700 (PDT) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:EM Lab manager position available, Naval Research Lab, 14, 40 -- Washington DC 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- References: {201509231243.t8NCh5TA025776-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Message-ID: {5606B25E.4040405-at-microscopy.com} 14, 40 -- Date: Sat, 26 Sep 2015 09:57:35 -0500 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.2.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201509231243.t8NCh5TA025776-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
As we are in the job for buying a new SEM with SDD, I come to the comunity with a few questions. Today I've a first question for the microanalysis experts :
There was a serie of interesting posts a few month ago about the accurracy of standardless microanalysis results (pointing in particular to a very usefull recent paper from D. Newbury and N. Ritchie ), and from my little experience, I agree in most what has been told. But in a material science and catalysis lab, there are much, much samples which cannot been polished, what is needed to do accurate quatitative analysis with standards.
Now, manufacturers propose configurations with 2 SDD detectors, positionned at ~90° one to the other. They claim it is usefull to get more signal with the same energy/current conditions (what is obvious), but too that this allows to cancel "more or less" the effect of the roughness on non-polished samples (I've added the "more or less". Vendors are much more optimistic). Of coarse, it will depend on the amplitude and shape of the roughness. But what else ? What are the traps and limits of such a configuration.
I'm interested to hear feedbacks and advices about the real interest of such a configuration, and about the reality of that cancelling effect. More ? Or less ? ;-)
Thanks
Jacques
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des MatĂŠriaux de Strasbourg DĂŠpartement Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Hello All The diffusion pump heater of our Jeol JSM5600-LV broke and we are trying to find a replacement. It is a 100V, 600W, 10.5 cm external diameter plate. I cannot see any label indicating which model of diffusion pump this Jeol scope uses. Will be thankful for any information about the type of pump and where to find this part, new or used. With best regards
Using 2 SDDs will help significantly but it will not eliminate the problem of surface roughness. You can look at two cases:
1) A perfectly polished sample, with the detectors at the same take-off angle and otherwise identical. Then they should see the same signal.
2) The side of a wall where the beam hits many microns below the top corner. In this case only one detector sees the signal, and the other will see none. Your ZAF correction will fail.
Realistic samples will be in between. So it is crucial to know what the scale of your sample roughness and approximate composition is. For example, consider an Fe-Ni alloy and the beam is hitting where the surface slopes by 10 degrees. In the Fe-Ni system, the Fe absorbs the Ni signal heavily and leads to big errors. I input 50 counts for each element into my software and then let it apply k-factors and a thin film correction (this is for TEM actually, but it will still give you a correct feel for the effect). This is a rough calculation so I assume a density of 10 g/cc (ballpark), a 30 degree takeoff and a micron thick film. With a 10 degree slope on the surface, one detector will see 20 degrees, and the other 40 degrees. The computed atomic percents are then:
20 deg takeoff Fe 45.8 at% Ni 54.2 at%
30 deg Fe 47.1 Ni 52.9
40 deg Fe 47.7 Ni 52.3
Relative error for one detector: abs(40deg - 30deg) / 30deg Fe 1% Ni 1%
Relative error for the other detector: abs(20deg - 30deg) / 30deg Fe 2.5% Ni 2.7%
(40deg + 20deg) / 2 / 30deg: Fe 0.8% error Ni 0.6% error relative
So if we assume your detector is at 30 degrees, the error one way or the other is a couple percent. But the average of them produces less than one percent error. So you see it helps quite a bit but doesnât eliminate the problem. As you can see, this is also very, very, very, morphology and composition dependent. I suggest you play around with it some using several materials and roughnesses you expect and get a feel for it.
I used my own software in this example called Stoichiometry Fitter, it is open source and free to use: https://github.com/ZGainsforth/StoichiometryFitter
Also, CASINO is superb for visualizing this stuff: http://www.gel.usherbrooke.ca/casino/What.html
I hope this helps,
Zack
} On Sep 28, 2015, at 3:24 AM, jacques.faerber-at-ipcms.u-strasbg.fr wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all } } As we are in the job for buying a new SEM with SDD, I come to the } comunity with a few questions. } Today I've a first question for the microanalysis experts : } } There was a serie of interesting posts a few month ago about the } accurracy of standardless microanalysis results (pointing in particular } to a very usefull recent paper from D. Newbury and N. Ritchie ), and } from my little experience, I agree in most what has been told. } But in a material science and catalysis lab, there are much, much } samples which cannot been polished, what is needed to do accurate } quatitative analysis with standards. } } Now, manufacturers propose configurations with 2 SDD detectors, } positionned at ~90° one to the other. They claim it is usefull to get } more signal with the same energy/current conditions (what is obvious), } but too that this allows to cancel "more or less" the effect of the } roughness on non-polished samples (I've added the "more or less". } Vendors are much more optimistic). } Of coarse, it will depend on the amplitude and shape of the roughness. } But what else ? What are the traps and limits of such a configuration. } } I'm interested to hear feedbacks and advices about the real interest of } such a configuration, and about the reality of that cancelling effect. } More ? Or less ? ;-) } } Thanks } } Jacques } } -- } } J. Faerber } IPCMS-DSI } Institut de Physique et Chimie des MatÊriaux de Strasbourg } DÊpartement Surfaces et Interfaces } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail : Jacques.Faerber-at-ipcms.unistra.fr } } } ==============================Original Headers============================== } 11, 25 -- From jacques.faerber-at-ipcms.u-strasbg.fr Mon Sep 28 05:08:45 2015 } 11, 25 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8SA8iXF018738 } 11, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Sep 2015 05:08:45 -0500 } 11, 25 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 11, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id DB3A424236C } 11, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Sep 2015 12:08:41 +0200 (CEST) } 11, 25 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr } 11, 25 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) } 11, 25 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) } 11, 25 -- with ESMTP id 5YxtiS-ih+hP for {microscopy-at-microscopy.com} ; } 11, 25 -- Mon, 28 Sep 2015 12:08:41 +0200 (CEST) } 11, 25 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) } 11, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id 1CDB5242387 } 11, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Sep 2015 12:08:41 +0200 (CEST) } 11, 25 -- To: microscopy-at-microscopy.com } 11, 25 -- From: jacques faerber {jacques.faerber-at-ipcms.u-strasbg.fr} } 11, 25 -- Subject: Microanalysis on SEM : 2 SDD configuration, advices } 11, 25 -- Message-ID: {560911A8.9080709-at-ipcms.u-strasbg.fr} } 11, 25 -- Date: Mon, 28 Sep 2015 12:08:40 +0200 } 11, 25 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:38.0) Gecko/20100101 } 11, 25 -- Thunderbird/38.2.0 } 11, 25 -- MIME-Version: 1.0 } 11, 25 -- Content-Type: text/plain; charset=utf-8; format=flowed } 11, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 21, 36 -- From zackg-at-berkeley.edu Mon Sep 28 12:05:29 2015 21, 36 -- Received: from mail-pa0-f52.google.com (mail-pa0-f52.google.com [209.85.220.52]) 21, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8SH5ScM011785 21, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Sep 2015 12:05:28 -0500 21, 36 -- Received: by pacfv12 with SMTP id fv12so183291016pac.2 21, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Sep 2015 10:05:25 -0700 (PDT) 21, 36 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 21, 36 -- d=1e100.net; s=20130820; 21, 36 -- h=x-gm-message-state:content-type:mime-version:subject:from 21, 36 -- :in-reply-to:date:content-transfer-encoding:message-id:references:to; 21, 36 -- bh=iBhGR9VBpsjwWktR2VZfIZEul7l6A6c9lM3xSAi1qoo=; 21, 36 -- b=T3mvdKYXIl/lpEBF98DtCTX/OuVNneyOIOAoVy+HczETp2OzUZP7gjiLccSnoUXkZS 21, 36 -- UhwC+z8UwNQLgI9ISgm5I6UlAxLb7N/ojYtJEo5vasWCyBsLBVzd7t+jlIp7i4JxGzS2 21, 36 -- X42zivtOe37CIbm6Xxk3odjLs6MiPPIwrR6mGKDQum4IR5UVhHAIKB3a6dGdl9V6XLQz 21, 36 -- c2tkuDotXbnRvlrcqkC5pnJWNLE8DmyY19rb5/+fGJr2GFlHfMkLVI/Dx+K2obVxFuqf 21, 36 -- 8/y3z/AkdMkvQ0K1mNPL6BYFvUlskeuLLWI2kjwA8Y0UH20RQvEObimNn2ofeNVz0r1w 21, 36 -- vlbQ== 21, 36 -- X-Gm-Message-State: ALoCoQlEMtyL3Q3itgO5SpzELfwjyi6O6xGpOhTbZmQqrmYobJb3Q/0iBC9LG4a+l2OJTz1gdf2c 21, 36 -- X-Received: by 10.68.68.233 with SMTP id z9mr27831795pbt.132.1443459925699; 21, 36 -- Mon, 28 Sep 2015 10:05:25 -0700 (PDT) 21, 36 -- Received: from continuum.home (pool-72-87-128-69.lsanca.fios.verizon.net. [72.87.128.69]) 21, 36 -- by smtp.gmail.com with ESMTPSA id li11sm20622794pab.43.2015.09.28.10.05.24 21, 36 -- (version=TLSv1/SSLv3 cipher=OTHER); 21, 36 -- Mon, 28 Sep 2015 10:05:25 -0700 (PDT) 21, 36 -- Content-Type: text/plain; charset=utf-8 21, 36 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 21, 36 -- Subject: Re: [Microscopy] Microanalysis on SEM : 2 SDD configuration, advices 21, 36 -- From: Zack Gainsforth {zackg-at-berkeley.edu} 21, 36 -- In-Reply-To: {201509281024.t8SAO2QB031565-at-ns.microscopy.com} 21, 36 -- Date: Mon, 28 Sep 2015 10:05:23 -0700 21, 36 -- Message-Id: {0596DCD0-3417-49C5-96FB-3BAD138E91D2-at-berkeley.edu} 21, 36 -- References: {201509281024.t8SAO2QB031565-at-ns.microscopy.com} 21, 36 -- To: jacques.faerber-at-ipcms.u-strasbg.fr, Microscopy-at-microscopy.com 21, 36 -- X-Mailer: Apple Mail (2.2104) 21, 36 -- Content-Transfer-Encoding: 8bit 21, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t8SH5ScM011785 ==============================End of - Headers==============================
From ohn.bullocknewseygot-at-gmail.com Tue Sep 29 04:21:59 2015 Return-Path: {ohn.bullocknewseygot-at-gmail.com} Received: from gmail.com ([121.190.223.4]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t8T9LusX028764 for {microscopylistserverarchive5-at-microscopy.com} ; Tue, 29 Sep 2015 04:21:58 -0500 Message-ID: {CB55E459.C99947B7-at-gmail.com}
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Organization: Chesapeake Microscopy and Microanalysis Society
Title-Subject: [Filtered] CMMS Fall Meeting
Message: Dear Friends & Colleagues,
The Chesapeake Microscopy & Microanalysis Society (CMMS) is pleased to announce our fall meeting to be held on October 13th from 4pm at the George Washington University in Washington, DC. There will be a tour of George Washington's new Nanofabrication and Imaging Center and dinner will be served.
Dr. Davi Bock (HHMI Janelia Research Campus) will be presenting the evenings talk entitled "Neuronal networks from large-scale electron microscopy of Drosophila".
Full meeting details and links to registration are available on the Society's website: http://csmicroscopy.org
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I need the services of a TEM microscopist skilled in the visualization of DNA, especially with respect to distinguishing between single-stranded and double-stranded forms. Incredibly, I am finding that almost no one knows how to do this anymore. Of the ~20 people/facilities I've contacted, no one even knows what the terms "hyperphase" and "hypophase" mean. If anyone at Microscopy ListServer knows how to perform EM studies of DNA, or can steer me to someone who does, I would be very grateful.
Ken Biegeleisen, MD, PhD
kb-at-notahelix.net https://notahelix.net
==============================Original Headers============================== 6, 29 -- From kb-at-notahelix.net Tue Sep 29 13:05:36 2015 6, 29 -- Received: from homiemail-a107.g.dreamhost.com (sub4.mail.dreamhost.com [69.163.253.135]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8TI5Zhp031481 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 29 Sep 2015 13:05:36 -0500 6, 29 -- Received: from homiemail-a107.g.dreamhost.com (localhost [127.0.0.1]) 6, 29 -- by homiemail-a107.g.dreamhost.com (Postfix) with ESMTP id 4B5002004F4F4 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 29 Sep 2015 11:05:33 -0700 (PDT) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha1; c=relaxed; d=notahelix.net; h=from:to 6, 29 -- :subject:date:message-id:mime-version:content-type 6, 29 -- :content-transfer-encoding; s=notahelix.net; bh=LVd237Z5KaCawnEB 6, 29 -- 5J+N8S5zyzo=; b=U+H2UCUaISRVUEyCpPw3mf6jhsjqZu3wDtGTfxP6r9TmVDrl 6, 29 -- cTfjpMWxwogLfN6Sh76cnLHAYzoz8M4lpPdGCeYg6Um08oROLdG3HTDygFc3FIlR 6, 29 -- 08Dt+vRxVgWzami1Ch7dh9emlPtovFPZKLnFv0fJSIJM2VvTzNs19YDJiJ8= 6, 29 -- Received: from kb1 (ool-4a59b5a0.dyn.optonline.net [74.89.181.160]) 6, 29 -- (Authenticated sender: kb-at-notahelix.net) 6, 29 -- by homiemail-a107.g.dreamhost.com (Postfix) with ESMTPA id E414F2004F4F2 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 29 Sep 2015 11:05:32 -0700 (PDT) 6, 29 -- From: "Not A Helix - Net" {kb-at-notahelix.net} 6, 29 -- To: {Microscopy-at-Microscopy.Com} 6, 29 -- Subject: Need microscopist skilled in TEM exam of single- and double-stranded DNA 6, 29 -- Date: Tue, 29 Sep 2015 14:05:28 -0400 6, 29 -- Message-ID: {032301d0fae1$6e55d990$4b018cb0$-at-net} 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; 6, 29 -- charset="us-ascii" 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- X-Mailer: Microsoft Office Outlook 12.0 6, 29 -- Thread-Index: AdD64WwOBgnjl92gQNS1o5Xz10o+4A== 6, 29 -- Content-Language: en-us ==============================End of - Headers==============================
I might be able to help you out Ken. I've cooked up a couple of hyperphases myself. Give me a buzz.
Best Regards, Larry Scipioni -- Larry Scipioni, PhD Vice President of R&D - Platform Development ZS Genetics les-at-zsgenetics.com 781-552-1989 (mobile) larry.scipioni (Skype) www.zsgenetics.com
-----Original Message----- X-from: kb-at-notahelix.net [mailto:kb-at-notahelix.net] Sent: Tuesday, September 29, 2015 2:21 PM To: LES-at-ZSGENETICS.COM
I need the services of a TEM microscopist skilled in the visualization of DNA, especially with respect to distinguishing between single-stranded and double-stranded forms. Incredibly, I am finding that almost no one knows how to do this anymore. Of the ~20 people/facilities I've contacted, no one even knows what the terms "hyperphase" and "hypophase" mean. If anyone at Microscopy ListServer knows how to perform EM studies of DNA, or can steer me to someone who does, I would be very grateful.
Ken Biegeleisen, MD, PhD
kb-at-notahelix.net https://notahelix.net
==============================Original Headers============================== 6, 29 -- From kb-at-notahelix.net Tue Sep 29 13:05:36 2015 6, 29 -- Received: from homiemail-a107.g.dreamhost.com (sub4.mail.dreamhost.com [69.163.253.135]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8TI5Zhp031481 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 29 Sep 2015 13:05:36 -0500 6, 29 -- Received: from homiemail-a107.g.dreamhost.com (localhost [127.0.0.1]) 6, 29 -- by homiemail-a107.g.dreamhost.com (Postfix) with ESMTP id 4B5002004F4F4 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 29 Sep 2015 11:05:33 -0700 (PDT) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha1; c=relaxed; d=notahelix.net; h=from:to 6, 29 -- :subject:date:message-id:mime-version:content-type 6, 29 -- :content-transfer-encoding; s=notahelix.net; bh=LVd237Z5KaCawnEB 6, 29 -- 5J+N8S5zyzo=; b=U+H2UCUaISRVUEyCpPw3mf6jhsjqZu3wDtGTfxP6r9TmVDrl 6, 29 -- cTfjpMWxwogLfN6Sh76cnLHAYzoz8M4lpPdGCeYg6Um08oROLdG3HTDygFc3FIlR 6, 29 -- 08Dt+vRxVgWzami1Ch7dh9emlPtovFPZKLnFv0fJSIJM2VvTzNs19YDJiJ8= 6, 29 -- Received: from kb1 (ool-4a59b5a0.dyn.optonline.net [74.89.181.160]) 6, 29 -- (Authenticated sender: kb-at-notahelix.net) 6, 29 -- by homiemail-a107.g.dreamhost.com (Postfix) with ESMTPA id E414F2004F4F2 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 29 Sep 2015 11:05:32 -0700 (PDT) 6, 29 -- From: "Not A Helix - Net" {kb-at-notahelix.net} 6, 29 -- To: {Microscopy-at-Microscopy.Com} 6, 29 -- Subject: Need microscopist skilled in TEM exam of single- and double-stranded DNA 6, 29 -- Date: Tue, 29 Sep 2015 14:05:28 -0400 6, 29 -- Message-ID: {032301d0fae1$6e55d990$4b018cb0$-at-net} 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; 6, 29 -- charset="us-ascii" 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- X-Mailer: Microsoft Office Outlook 12.0 6, 29 -- Thread-Index: AdD64WwOBgnjl92gQNS1o5Xz10o+4A== 6, 29 -- Content-Language: en-us ==============================End of - Headers==============================
==============================Original Headers============================== 18, 51 -- From les-at-zsgenetics.com Tue Sep 29 13:26:23 2015 18, 51 -- Received: from gateway26.websitewelcome.com (gateway26.websitewelcome.com [192.185.15.239]) 18, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t8TIQNp1019760 18, 51 -- for {microscopy-at-microscopy.com} ; Tue, 29 Sep 2015 13:26:23 -0500 18, 51 -- Received: by gateway26.websitewelcome.com (Postfix, from userid 1000) 18, 51 -- id 2A0EE78C970A; Tue, 29 Sep 2015 13:26:21 -0500 (CDT) 18, 51 -- Received: from cm2.websitewelcome.com (unknown [192.185.178.13]) 18, 51 -- by gateway26.websitewelcome.com (Postfix) with ESMTP id 26EC778C8BD8 18, 51 -- for {microscopy-at-microscopy.com} ; Tue, 29 Sep 2015 13:26:21 -0500 (CDT) 18, 51 -- Received: from gator2013.hostgator.com ([50.87.144.13]) 18, 51 -- by cm2.websitewelcome.com with 18, 51 -- id P6SJ1r00J0HZY1u016SKtn; Tue, 29 Sep 2015 13:26:21 -0500 18, 51 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; d=zsgenetics.com; s=default; 18, 51 -- h=Content-Transfer-Encoding:Content-Type:MIME-Version:Message-ID:Date:Subject:In-Reply-To:References:Cc:To:From; bh=MgNrO+jwE7sj1P0lXjKHpHiVujqFe6MB6MRSUxDsFbE=; 18, 51 -- b=I02tT/4GHZLxIaRnYKFLMxx5rVJZd2PqvfAysamVfw8qMTi096LAcnccorEi53nZM3bfl4saZx0Vf8aYK6PLHeAb9Hi2+fpepSqmFohWoRHyCzgTOV11ZUDw4rQqX7CiqvpIYw9czZzXf2HhrogJ4n4mU/3LL5p7lwLtIn3dpoo=; 18, 51 -- Received: from [146.115.5.114] (port=49496 helo=LESZSG) 18, 51 -- by gator2013.hostgator.com with esmtpsa (TLSv1:AES256-SHA:256) 18, 51 -- (Exim 4.85) 18, 51 -- (envelope-from {les-at-zsgenetics.com} ) 18, 51 -- id 1ZgzbV-000QTW-MC; Tue, 29 Sep 2015 13:26:17 -0500 18, 51 -- From: "Larry Scipioni" {les-at-zsgenetics.com} 18, 51 -- To: {kb-at-notahelix.net} 18, 51 -- Cc: {microscopy-at-microscopy.com} 18, 51 -- References: {201509291820.t8TIKeMp014964-at-ns.microscopy.com} 18, 51 -- In-Reply-To: {201509291820.t8TIKeMp014964-at-ns.microscopy.com} 18, 51 -- Subject: RE: [Microscopy] Need microscopist skilled in TEM exam of single- and double-stranded DNA 18, 51 -- Date: Tue, 29 Sep 2015 14:26:14 -0400 18, 51 -- Organization: ZS Genetics 18, 51 -- Message-ID: {000f01d0fae4$546b9ad0$fd42d070$-at-zsgenetics.com} 18, 51 -- MIME-Version: 1.0 18, 51 -- Content-Type: text/plain; 18, 51 -- charset="us-ascii" 18, 51 -- Content-Transfer-Encoding: 7bit 18, 51 -- X-Mailer: Microsoft Outlook 14.0 18, 51 -- Thread-Index: AQJ6jGJ0PsPKvWpctPkA+VSUWzR68p0Arx4g 18, 51 -- Content-Language: en-us 18, 51 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 18, 51 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com 18, 51 -- X-AntiAbuse: Original Domain - microscopy.com 18, 51 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 18, 51 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com 18, 51 -- X-BWhitelist: no 18, 51 -- X-Source-IP: 146.115.5.114 18, 51 -- X-Exim-ID: 1ZgzbV-000QTW-MC 18, 51 -- X-Source: 18, 51 -- X-Source-Args: 18, 51 -- X-Source-Dir: 18, 51 -- X-Source-Sender: (LESZSG) [146.115.5.114]:49496 18, 51 -- X-Source-Auth: les-at-zsgenetics.com 18, 51 -- X-Email-Count: 16 18, 51 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20= ==============================End of - Headers==============================
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Email: chrisbrantner-at-email.gwu.edu Name: Chris Brantner
Organization: George Washington University Nanofabrication and Imaging Center
Title-Subject: [Filtered] preparing Lowicryl K4M or HM20
Message: Good afternoon all,
I need to prepare some Lowicryl. I am leaning towards K4M. I have never done this. Can someone give me a few pointers? The recipe says a brown glass container. But these are pricey containers to purchase for mixing up resin. I would like to use this in my freeze substitution device on some heart tissue for immunolabeling. If I am using ethanol in my resin mixtures, should I use ethanol in my substitution medium instead of acetone?
Thank you Chris
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From mike.sfsd4f564s6df45dszqzuu-at-gmail.com Wed Sep 30 19:23:39 2015 Return-Path: {mike.sfsd4f564s6df45dszqzuu-at-gmail.com} Received: from gmail.com ([125.137.207.137]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t910Na2K029102 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 30 Sep 2015 19:23:38 -0500 Message-ID: {52B308F6.DD6D53AD-at-gmail.com}
Chris, I have used Lowicryl K4M, LR White and Gold and HM20). I have found HM20 to be superior in: Infiltration, preparation, sectioning and immuno-labelling. I would recommend it over the others, you can make it up in a glass scintillation vial (mixing with light N2 bubbling). I have also found that acetone is the best solvent for IEM, I have tried both methanol and ethanol-too much extraction. We have done the HPF hybrid technique (light pre-fixation in phosphate buffered paraformaldehyde-rinse-HPF in hexadecene-LN2 storage), followed by AFS. Quickly my protocol is AFS in IEM cocktail (0.2% GA, 0.2% UA in 95% Acetone (5% D-H20) at -90C. This is followed by gradual increase (slope 5 degrees/hr) to -45C, then held there for solvent rinse (3 x 10 min pure Acetone) followed by 50% then 75% HM20 in Acetone. After and overnight in pure HM20, I change to fresh resin once (2hrs) then again right before UV illumination. Sections are picked up on formvar coated nickel or slot grids and immuno-labeled by floating on drops of: NH4Cl, block, primarly Aby overnight 4c, TBS rinse, Au conjugated secondary Aby (2 hrs RT), rinse, hard GA fix, rinse, UA stain rinse and dry. No first Aby sections followed by secondary Aby serves as negative control. The key is to make sure the tissue blocks are small and fit into the planchets, we usually use two 300 um depth planchets filled with 1.2 ul of hexadecene. When sectioning, due to the fact that a good freeze is within a 200 um depth of the tissue, we concentrate on the periphery of the tissue, not the middle. I hope this helps, Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, September 30, 2015 6:19 PM To: mdelann1-at-jhmi.edu
X-from: chrisbrantner-at-email.gwu.edu
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Email: chrisbrantner-at-email.gwu.edu Name: Chris Brantner
Organization: George Washington University Nanofabrication and Imaging Center
Title-Subject: [Filtered] preparing Lowicryl K4M or HM20
Message: Good afternoon all,
I need to prepare some Lowicryl. I am leaning towards K4M. I have never done this. Can someone give me a few pointers? The recipe says a brown glass container. But these are pricey containers to purchase for mixing up resin. I would like to use this in my freeze substitution device on some heart tissue for immunolabeling. If I am using ethanol in my resin mixtures, should I use ethanol in my substitution medium instead of acetone?
Thank you Chris
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Dear Listers, Back on Sept. 14 I posted a question about how to explain to researchers the difficulty of quantifying autophagosomes seen in electron micrographs and I have requests to post a summary of replies. This is the original question I posted: "I'm getting an increase in researchers coming to me wanting to see autophagosomes (the last few years it seems that autophagy is becoming quite a popular topic). Getting images of autophagosomes is not a problem. The problem I am having with researchers, is getting them to understand that you cannot quantify the presence of the autophagosomes by simply counting the ones you see in a few sections. When I try to explain about stereology methods we could use to quantify the autophagosomes I lose them, in addition I keep getting presented with papers they have read in which the quantitative methods being used are clearly wrong. I would like to hear from others who are dealing with similar requests and how are you handling these requests. In particular anyone who is involved in quantifying autophagosomes by using TEM. As always thanks in advance, all help is appreciated."
The short answer is I am obviously not alone in trying to explain to researchers you simply can't count individual autophagosomes in one or two sections and say you have statistically quantifiable results. I understand that it is proper etiquette not to post individual's names, so I will provide a few of the comments I received without attribution.
"You can lead a horse to water, but you cannot make him drink. I've had the same experience and I'm losing the battle."
"I feel your pain. We don't have a solution."
"I had the same problem and when we tried to tell them of the bad math they would not believe us "because it was published "."
Basically, all of the responses were along the same vein as the above quotes. My thanks to everyone and especially to the authors of the above quotes. Following are a couple of quotes with useful tips. "One suggestion for you that has helped me is assembling a brief PowerPoint that you put into a PDF format and share with the folks approaching you which succinctly illustrates the issue and the need for another approach. This way, you don't have to keep explaining the same thing repeatedly to folks. " This I am going to do.
"I think that once they have a good label for confocal Microscopy all could be counted in a number of cells in different cell populations. That's what's needed for statistics. " The above point is valid, but only for cell cultures examined in by Confocal. Still leaves us with the problem of sections of tissues. Also, I was asked to share what I have done. I do have some experience with stereology. I was fortunate to be able to attend the 8th ISS American Stereology Course in 2004 in Minneapolis. If you have the opportunity to attend one of these, do so. The individual who organized this one is an expert in stereology, if you wish to contact this individual, please contact me privately and I will pass your request on to that individual. In addition I have a well worn copy of "Unbiased Stereology, Three Dimensional Measurement in Microscopy" by C. V. Howard and M. G. Reed. If you want to learn some stereology on your own this is the book to buy. The project that prompted my original question, was not well suited for an attempt at quantification. I have another researcher with a project whose sample will be well suited for an attempt at quantification of autophagosomes. For this one I will be using one of the number estimation techniques detailed in chapter 5 of Howard and Reed's textbook. Which will estimate numerical density in relation to the reference volume. I hope this summary has adequately addressed people's requests for feedback.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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Title-Subject: [Filtered] Northern California Society for Microscopy Meeting
Message: The Northern California Society for Microscopy's Fall Meeting is planned for Thursday, October 29, 6-9 pm at Nanolab Technologies in Milpitas, CA. Details and membership information is available at ncsmicroscopy.org We have a great line up of speakers and hope you can join us.
Roseann NCSM president
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Message: Hi, Do you clean Nickel Grids the same way you clean copper grids, with 0.1N HCL, 100% ETOH, 100% Acetone? I ask because I wasn't sure if the nickel would oxidize, "tarnish" or flake off with these three chemicals?
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Title-Subject: [Filtered] Help working with a MillicellŽ Hanging Membrane Inserts
Message: Does anyone out there have experience working with the membrane of the MillicellŽ Cell Culture Hanging Inserts. The EM technician is having a hard time sectioning these membranes for thick and thin sections. The biggest problem are wrinkling of the membrane. The membranes are embedded in Polybed 812 (I think this is the problem) but she has tried xylene and chloroform but neither will remove the wrinkles. HELP. Thanks in advance. Connie
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA -ERRC
Title-Subject: [Filtered] SEM of leather
Message: I'm looking for suggestions on how to prepare leather for SEM viewing. (tanned & untanned, tanned is worse for charging) Currently I slice a cross section by hand as thin as possible. Mount with a small dab of Duco cement, and silver paint the edges. (Tried mounting with a dab of silver paint- not much improvement).
The corium takes gold coating reasonably well but the grain seems to be resistant to all but LONG sputter coating. I get charging at higher magnifications.
Would a different metal coating do better? Perfuse some conductive material into the sample? tho that might disturb structure.
thanks
Joe
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Mon Oct 5 18:14:49 2015 14, 40 -- Received: from mail-qg0-f52.google.com (mail-qg0-f52.google.com [209.85.192.52]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t95NEnjg016485 14, 40 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2015 18:14:49 -0500 14, 40 -- Received: by qgx61 with SMTP id 61so164147987qgx.3 14, 40 -- for {microscopy-at-microscopy.com} ; Mon, 05 Oct 2015 16:14:46 -0700 (PDT) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=5dBnDZF8Vj6WQBKRvUJ6i5bs3epb4tuJObhg4FwshEE=; 14, 40 -- b=uNLYJSB4ANaBWUg7J5ohzd1L9NJGnWEBGRcEuhz4QWAejYTEDI1VscwrmjBNECsq/k 14, 40 -- RUT1uSQ3lO5GnR5zIKfQdigoeULv9jb4gRVuB+U7FD2XI1CXx4yfUksYT+EmuEHTih1E 14, 40 -- B6X/Y8iYJBUgV2ur73fIc54IGT33+aDRgONfaDsvAuLjsRGfh3IhXAL36SQNd75MOf9Q 14, 40 -- k1cBsFk5qgQALpcmdSpoEQUrAXDEJ7TEg0voIIANmKAf9kxm6g6fs2JJlZGAqqXuTU4Y 14, 40 -- PgJ84DVRxUJ+UWjCBNONThoc+95WoXUKX7l/HxINpmlzcJXwTYOiB/whztrtlDw09UqN 14, 40 -- 1j8Q== 14, 40 -- X-Received: by 10.140.18.240 with SMTP id 103mr44189231qgf.31.1444086886546; 14, 40 -- Mon, 05 Oct 2015 16:14:46 -0700 (PDT) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id b91sm12457986qge.8.2015.10.05.16.14.45 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 40 -- Mon, 05 Oct 2015 16:14:46 -0700 (PDT) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:SEM of leather 14, 40 -- References: {201510051753.t95Hr7bW007429-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201510051753.t95Hr7bW007429-at-ns.microscopy.com} 14, 40 -- Message-ID: {56130465.8050306-at-microscopy.com} 14, 40 -- Date: Mon, 5 Oct 2015 18:14:45 -0500 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.2.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201510051753.t95Hr7bW007429-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
My first question would be what accelerating voltage are you using, and what spot size when compared with your normal operating conditions? With this information we may move forward.
Come back to me.
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
---------------------------------------------------------------------------- ----------------------------------------------- Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA -ERRC
Title-Subject: [Filtered] SEM of leather
Message: I'm looking for suggestions on how to prepare leather for SEM viewing. (tanned & untanned, tanned is worse for charging) Currently I slice a cross section by hand as thin as possible. Mount with a small dab of Duco cement, and silver paint the edges. (Tried mounting with a dab of silver paint- not much improvement).
The corium takes gold coating reasonably well but the grain seems to be resistant to all but LONG sputter coating. I get charging at higher magnifications.
Would a different metal coating do better? Perfuse some conductive material into the sample? tho that might disturb structure.
thanks
Joe
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Mon Oct 5 18:14:49 2015 14, 40 -- Received: from mail-qg0-f52.google.com (mail-qg0-f52.google.com [209.85.192.52]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t95NEnjg016485 14, 40 -- for {microscopy-at-microscopy.com} ; Mon, 5 Oct 2015 18:14:49 -0500 14, 40 -- Received: by qgx61 with SMTP id 61so164147987qgx.3 14, 40 -- for {microscopy-at-microscopy.com} ; Mon, 05 Oct 2015 16:14:46 -0700 (PDT) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=5dBnDZF8Vj6WQBKRvUJ6i5bs3epb4tuJObhg4FwshEE=; 14, 40 -- b=uNLYJSB4ANaBWUg7J5ohzd1L9NJGnWEBGRcEuhz4QWAejYTEDI1VscwrmjBNECsq/k 14, 40 -- RUT1uSQ3lO5GnR5zIKfQdigoeULv9jb4gRVuB+U7FD2XI1CXx4yfUksYT+EmuEHTih1E 14, 40 -- B6X/Y8iYJBUgV2ur73fIc54IGT33+aDRgONfaDsvAuLjsRGfh3IhXAL36SQNd75MOf9Q 14, 40 -- k1cBsFk5qgQALpcmdSpoEQUrAXDEJ7TEg0voIIANmKAf9kxm6g6fs2JJlZGAqqXuTU4Y 14, 40 -- PgJ84DVRxUJ+UWjCBNONThoc+95WoXUKX7l/HxINpmlzcJXwTYOiB/whztrtlDw09UqN 14, 40 -- 1j8Q== 14, 40 -- X-Received: by 10.140.18.240 with SMTP id 103mr44189231qgf.31.1444086886546; 14, 40 -- Mon, 05 Oct 2015 16:14:46 -0700 (PDT) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id b91sm12457986qge.8.2015.10.05.16.14.45 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 40 -- Mon, 05 Oct 2015 16:14:46 -0700 (PDT) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:SEM of leather 14, 40 -- References: {201510051753.t95Hr7bW007429-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201510051753.t95Hr7bW007429-at-ns.microscopy.com} 14, 40 -- Message-ID: {56130465.8050306-at-microscopy.com} 14, 40 -- Date: Mon, 5 Oct 2015 18:14:45 -0500 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.2.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201510051753.t95Hr7bW007429-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 18, 22 -- From protrain-at-emcourses.com Tue Oct 6 04:38:36 2015 18, 22 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 18, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t969cahg013133 18, 22 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2015 04:38:36 -0500 18, 22 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 18, 22 -- id {B5613968f0000} ; Tue, 06 Oct 2015 10:38:23 +0100 18, 22 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Tue, 6 Oct 2015 10:38:22 +0100 18, 22 -- From: "Steve Chapman" {protrain-at-emcourses.com} 18, 22 -- To: {joseph.uknalis-at-ars.usda.gov} 18, 22 -- Cc: {microscopy-at-microscopy.com} 18, 22 -- References: {201510052317.t95NHa9E018213-at-ns.microscopy.com} 18, 22 -- In-Reply-To: {201510052317.t95NHa9E018213-at-ns.microscopy.com} 18, 22 -- Subject: RE: [Microscopy] viaWWW:SEM of leather 18, 22 -- Date: Tue, 6 Oct 2015 10:38:27 +0100 18, 22 -- Message-ID: {003501d1001a$c14c0730$43e41590$-at-com} 18, 22 -- MIME-Version: 1.0 18, 22 -- Content-Type: text/plain; 18, 22 -- charset="us-ascii" 18, 22 -- Content-Transfer-Encoding: 7bit 18, 22 -- X-Mailer: Microsoft Office Outlook 12.0 18, 22 -- Thread-Index: AdD/xABzK2BmKlIYSiO2f3xc9/MMXAAVjkDQ 18, 22 -- Content-Language: en-gb ==============================End of - Headers==============================
We have all seen łpepper˛ at some time in our careers and usually it can be attributed to incomplete washing of samples after glutaraldehyde fixation and before osmium, staining problems, and lots of unknowns. I have some recent samples that exhibited small black dots of similar size throughout an isolated membrane preparation. The dots are visible prior to staining so staining can be ruled out. The background resin is always clean.
The sample pellets were fixed with fresh glutaraldehyde in Sorensonšs phosphate buffer washed repeatedly and then post fixed in osmium, resuspended in each solution change. Percentages were same as I have used for 40 years. Pellets were agarose enrobed and resultant material cut into blocks prior to dehydration in ETOH series followed by propylene oxide and EPON generic embedding.
Nothing out of routine was done. However, the samples were prepared using a Percoll gradient. Is it possible that some of the Percoll was attached to the membranes and gave the dark spots that lined the membranes? It consists of colloidal silica particles coated with polyvinylpyrrolidone (PVP). I have little experience with Percoll having primarily done pellets purified through sucrose gradients in the past. If it is Percoll that attaches to the membranes, is there any way to efficiently wash it off?
Any ideas or suggestions?
Debby
==============================Original Headers============================== 8, 31 -- From dsherman-at-purdue.edu Tue Oct 6 08:12:14 2015 8, 31 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t96DCEAw011659 8, 31 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2015 08:12:14 -0500 8, 31 -- Received: from WPVEXCHUB02.purdue.lcl (wpvexchub02.itap.purdue.edu [172.30.136.64]) 8, 31 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id t96DCB3e005226 8, 31 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2015 09:12:12 -0400 8, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([172.30.136.65]) by 8, 31 -- WPVEXCHUB02.purdue.lcl ([172.30.136.64]) with mapi id 14.03.0224.002; Tue, 6 8, 31 -- Oct 2015 09:12:11 -0400 8, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 8, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 31 -- Subject: pepper in membrane pellets 8, 31 -- Thread-Topic: pepper in membrane pellets 8, 31 -- Thread-Index: AQHRADibXoOV60ZOZk2jx34TJksoJA== 8, 31 -- Date: Tue, 6 Oct 2015 13:12:10 +0000 8, 31 -- Message-ID: {D23940DC.331DF%dsherman-at-purdue.edu} 8, 31 -- Accept-Language: en-US 8, 31 -- Content-Language: en-US 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- user-agent: Microsoft-MacOutlook/14.5.5.150821 8, 31 -- x-originating-ip: [68.50.199.160] 8, 31 -- Content-Type: text/plain; charset="iso-8859-1" 8, 31 -- Content-ID: {6E75407A2447F441868572C560D4EAAD-at-exchange.purdue.edu} 8, 31 -- MIME-Version: 1.0 8, 31 -- X-PMX-Version: 6.0.2.2308539 8, 31 -- X-PerlMx-URL-Scanned: Yes 8, 31 -- X-PerlMx-Virus-Scanned: Yes 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t96DCEAw011659 ==============================End of - Headers==============================
We invite everyone to come and join us for a day of lectures by top in their field scientists, a student competition, vendor fair and food, November 4th at the U of Illinois Urbana-Champaign Campus, Materials Research Laboratory Fall workshop.
November 4th: Lectures, Vendors, Student Competition November 5th: Tours, open lab in Biological TEM prep lab.
Students, attendees Information and registration: http://mrl.illinois.edu/mrl-biological-conference-2015 $20 per attendee, participants in the competition have their fee refunded.
VENDORSâ Exhibitor registration: my.mrl.illinois.edu/eventreg/ $200 per table, one day, welcome to stay and demonstrate instruments the second day.
DEADLINES: October 15th for student competition participation. Information at: http://mrl.illinois.edu/mrl-biological-conference-2015
SPEAKERS: Ka Yee LeeChemistry- U of Chicago Speaking on the biophysics of proteins and lipids
Elizabeth Driskell â Veterinary Medicine âCellular and Organ Ultrastructureâ
Rashid Bashir â Bioengineering âBuilding Biological Machines with Living Cellsâ
Brian Cunningham â Bioengineering âQuantitative Imaging of Live Cell Adhesion by Photonic Crystal Enhanced Microscopyâ
Ryan Bailey â Chemistry "Translational Biomedical Microdevices"
Rohit Bhargava â Bioengineering âChemical Imaging for Molecular and Materials Analysis in Cancerâ
Hope you can join us!
Lou Ann Miller
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Hours: 7am-3:30 pm
The Duco cement isn't helping you - you're mounting with an insulator. I would use either a conductive carbon tab/tape *and* silver paint around the edges. Then sputter coat, using 60/40 gold/palladium. Cheaper than pure gold and it gives a better (finer grain coat).
More importantly, what kV and spot size are you using? Reducing both will go a long way to reducing or eliminating charging. This may be all you need to do.
You could also try adding osmium vapor "fixation". Place the sample in a petri dish and a crystal of OsO4, or a drop of 2-4% OsO4 in water in a smaller dish, so the OsO4 doesn't contact the sample. Don't cover the same dish with the OsO4 but cover and parafilm sealed the big dish. Let sit at room temp overnight - 24 hrs. The OsO4 doesn't bind that well to proteins, but it will still bind.
Phil
} Email: joseph.uknalis-at-ars.usda.gov } Name: Joe Uknalis } } Organization: USDA -ERRC } } Title-Subject: [Filtered] SEM of leather } } Message: } I'm looking for suggestions on how to prepare leather for SEM viewing. (tanned& untanned, tanned is } worse for charging) Currently I slice a cross section by hand as thin as possible. Mount with a } small dab of Duco cement, and silver paint the edges. (Tried mounting with a dab of silver paint- } not much improvement). } } The corium takes gold coating reasonably well but the grain seems to be resistant to all but LONG } sputter coating. I get charging at higher magnifications. } } Would a different metal coating do better? } Perfuse some conductive material into the sample? tho that might disturb structure. } } thanks } } Joe -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 35 -- From oshel1pe-at-cmich.edu Tue Oct 6 08:47:40 2015 6, 35 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t96DldfO019253 6, 35 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2015 08:47:39 -0500 6, 35 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 6, 35 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t96DlZo4008727; 6, 35 -- Tue, 6 Oct 2015 09:47:35 -0400 6, 35 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 6, 35 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 6, 35 -- (TLS) id 14.3.248.2; Tue, 6 Oct 2015 09:47:34 -0400 6, 35 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 6, 35 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 6, 35 -- (TLS) id 14.3.248.2; Tue, 6 Oct 2015 09:47:34 -0400 6, 35 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 6, 35 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 6, 35 -- 14.3.248.2; Tue, 6 Oct 2015 09:47:33 -0400 6, 35 -- Message-ID: {5613D0F5.3020409-at-cmich.edu} 6, 35 -- Date: Tue, 6 Oct 2015 09:47:33 -0400 6, 35 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 35 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 35 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 35 -- MIME-Version: 1.0 6, 35 -- To: micro {microscopy-at-microscopy.com} , {joseph.uknalis-at-ars.usda.gov} 6, 35 -- Subject: Re: [Microscopy] viaWWW:SEM of leather 6, 35 -- References: {201510060004.t9604E91017549-at-ns.microscopy.com} 6, 35 -- In-Reply-To: {201510060004.t9604E91017549-at-ns.microscopy.com} 6, 35 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 35 -- Content-Transfer-Encoding: 7bit 6, 35 -- X-Originating-IP: [141.209.2.100] 6, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 35 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 6, 35 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 6, 35 -- X-CanItPRO-Stream: default 6, 35 -- X-Canit-Stats-ID: 05PpNLzla - d3b2f2f8e1cb - 20151006 6, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Microscopy Society of NE Ohio (MSNO)
Title-Subject: [Filtered] 2015 MSNO Fall meeting -3 D printing - Wed Oct 14 3-7:30 at Jones Meeting Room, Kilcawley Center, Youngstown State University pm
Message: Dear all,
Our MSNO Fall Meeting 2015 will go to a new and exciting place: Youngstown State University, partly thanks to the hospitality of Dr. Dingqiang Li, Dr. Timothy Wagner and others at YSU. Please check the detailed program at http://www.msneo.org/uploads/3/1/7/7/31779867/msno-fallmeetingflyer-2015.pdf.
This will also be our first attempt to upgrade our traditional fall meeting format and add even more value (for example, to enhance the educational and student career networking functions) to the program. The event will combine professional networking, lectures on fast growing technologies by world experts, guided tours & demos at two outstanding YSU facilities, exhibitions by leading vendors in the field, a nice dinner, a fun raffle and much more. Here are some of the highlights:
Two main talks on additive manufacture (3D-printing, by Dr. James McGuffin-Cawley, MSE Chair at CWRU) and focused ion beam (FIB, by Dr. Lucille A. Giannuzzi, National MAS tour speaker with unique academic and industrial experiences). Many thanks go to Dr. Valerie Woodward and National MAS for arranging Dr Giannuzzi to fly to NEO for this event.
Tours & demos at Center for Innovation in Additive Manufacturing (3D-printing), and Electron Microscopy facility. Basics on 3D-printing and electron microscopy (including FIB) will be introduced. A professional table will be set up as our first effort to enhance the interaction between students and industrial and academic professionals (experienced or new). MSNO Student Awards: two winners of 2015 will be presented with certificates and monetary awards. Several winners will be randomly drawn from our attendees at the RAFFLE.
Online registration is available at http://www.msneo.org/2015-fall-meeting-registration.html
Looking forward to seeing you at YSU! MSNO Board
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA-ERRC
Title-Subject: [Filtered] SEM of leather, thanks for feedback
Message: Thanks everyone for the feedback.
I was using 10kv spot 3 on a FEI 200 FEG I dropped to 5kv but the charging got worse ???
Unfortunately I don't have a carbon coating option. Low vac/ESEM has poor resolution at 50,000X.
Will try the osmium vapor. Gold/palladium is more $$ than gold alone, may give that a try. Will try different spot sizes.
Unfortunately my email bounces reply to the list & I have to submit via the web based form.
thanks again
Joe
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==============================Original Headers============================== 17, 40 -- From microscopy.listserver-at-gmail.com Tue Oct 6 18:27:10 2015 17, 40 -- Received: from mail-qg0-f53.google.com (mail-qg0-f53.google.com [209.85.192.53]) 17, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t96NRARF023930 17, 40 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2015 18:27:10 -0500 17, 40 -- Received: by qgx61 with SMTP id 61so697926qgx.3 17, 40 -- for {microscopy-at-microscopy.com} ; Tue, 06 Oct 2015 16:27:08 -0700 (PDT) 17, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 40 -- d=gmail.com; s=20120113; 17, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 17, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 17, 40 -- bh=BnMh7ukr5rLvRFQ3HIyKVOSl7WA56IOEgIjrCBPYx/0=; 17, 40 -- b=JugepLV7bshBeoCDcQ3+YF7vFfF9owD2HQOSnjrdDQc2pMOg20QiEH/HOsEtfzVBxb 17, 40 -- XDHpxHLFIe8/yYjeiVRcSi0pkVYcYTex+wNeB11Lvm3oeUMIxiHXvxu1Y1LzNESlLNwJ 17, 40 -- XbRKUuw08Rg9G4MVzsHuwsVOpOXzeTQKg9GeIuEKXLstb5MLcszH+skULlrSi0LNqk8o 17, 40 -- Z3Cy/dN4WHc/UTfPaT0p205UI8H05MME9sIelJBtjL4VUFVLxrh8BZDKz2YPbjQjk6QY 17, 40 -- 400uqJilYbBGgbxJUr32WEBMFlh7zcbvIK+exAFMbf9BPIleKJmsfDiYx3eEcHg0S2q1 17, 40 -- X6XQ== 17, 40 -- X-Received: by 10.140.102.53 with SMTP id v50mr50481189qge.33.1444174028083; 17, 40 -- Tue, 06 Oct 2015 16:27:08 -0700 (PDT) 17, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 17, 40 -- by smtp.googlemail.com with ESMTPSA id e6sm14989821qga.14.2015.10.06.16.27.07 17, 40 -- for {microscopy-at-microscopy.com} 17, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 17, 40 -- Tue, 06 Oct 2015 16:27:07 -0700 (PDT) 17, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 17, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 17, 40 -- {microscopylistserver-noreply-at-microscopy.com} 17, 40 -- Subject: viaWWW:SEM of leather, thanks for feedback 17, 40 -- References: {201510061758.t96Hw72s015940-at-ns.microscopy.com} 17, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 40 -- X-Forwarded-Message-Id: {201510061758.t96Hw72s015940-at-ns.microscopy.com} 17, 40 -- Message-ID: {561458CB.7070000-at-microscopy.com} 17, 40 -- Date: Tue, 6 Oct 2015 18:27:07 -0500 17, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 17, 40 -- Gecko/20100101 Thunderbird/38.2.0 17, 40 -- MIME-Version: 1.0 17, 40 -- In-Reply-To: {201510061758.t96Hw72s015940-at-ns.microscopy.com} 17, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would start at about 800V and work my way up. I try to get to the point where the electrons in (beam) are close to the electrons out (SEI, BSE, etc.), and I have the best resolution I can, with minimal charging. With the FEG, I think you will be able to get the mag you want, though I am not familiar with your SEM. I start at low kV and work my way up. If you start high and go down, you will never see that area as best as you could have.
I hope this made some sense. Regards, Darrell
microscopy.listserver-at-gmail.com wrote on 10/06/2015 07:27:52 PM:
} From: microscopy.listserver-at-gmail.com } To: Darrell Miles/Fishkill/IBM-at-IBMUS } Date: 10/06/2015 07:27 PM } Subject: [Microscopy] viaWWW:SEM of leather, thanks for feedback } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: joseph.uknalis-at-ars.usda.gov } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both joseph.uknalis-at-ars.usda.gov as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: joseph.uknalis-at-ars.usda.gov } Name: Joe Uknalis } } Organization: USDA-ERRC } } Title-Subject: [Filtered] SEM of leather, thanks for feedback } } Message: Thanks everyone for the feedback. } } I was using 10kv spot 3 on a FEI 200 FEG } I dropped to 5kv but the charging got worse ??? } } Unfortunately I don't have a carbon coating option. } Low vac/ESEM has poor resolution at 50,000X. } } Will try the osmium vapor. } Gold/palladium is more $$ than gold alone, may give that a try. } Will try different spot sizes. } } Unfortunately my email bounces reply to the list & I have to submit } via the web based form. } } thanks again } } Joe } } } Login Host: 199.133.43.218 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original Headers============================== } 17, 40 -- From microscopy.listserver-at-gmail.com Tue Oct 6 18:27:10 2015 } 17, 40 -- Received: from mail-qg0-f53.google.com (mail-qg0- } f53.google.com [209.85.192.53]) } 17, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id t96NRARF023930 } 17, 40 -- for {microscopy-at-microscopy.com} ; Tue, 6 Oct 2015 18:27:10 -0500 } 17, 40 -- Received: by qgx61 with SMTP id 61so697926qgx.3 } 17, 40 -- for {microscopy-at-microscopy.com} ; Tue, 06 Oct 2015 } 16:27:08 -0700 (PDT) } 17, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 17, 40 -- d=gmail.com; s=20120113; } 17, 40 -- h=from:subject:references:to:reply-to:message- } id:date:user-agent } 17, 40 -- :mime-version:in-reply-to:content-type:content- } transfer-encoding; } 17, 40 -- bh=BnMh7ukr5rLvRFQ3HIyKVOSl7WA56IOEgIjrCBPYx/0=; } 17, 40 -- b=JugepLV7bshBeoCDcQ3 } +YF7vFfF9owD2HQOSnjrdDQc2pMOg20QiEH/HOsEtfzVBxb } 17, 40 -- XDHpxHLFIe8/yYjeiVRcSi0pkVYcYTex } +wNeB11Lvm3oeUMIxiHXvxu1Y1LzNESlLNwJ } 17, 40 -- } XbRKUuw08Rg9G4MVzsHuwsVOpOXzeTQKg9GeIuEKXLstb5MLcszH+skULlrSi0LNqk8o } 17, 40 -- Z3Cy/dN4WHc/ } UTfPaT0p205UI8H05MME9sIelJBtjL4VUFVLxrh8BZDKz2YPbjQjk6QY } 17, 40 -- 400uqJilYbBGgbxJUr32WEBMFlh7zcbvIK } +exAFMbf9BPIleKJmsfDiYx3eEcHg0S2q1 } 17, 40 -- X6XQ== } 17, 40 -- X-Received: by 10.140.102.53 with SMTP id } v50mr50481189qge.33.1444174028083; } 17, 40 -- Tue, 06 Oct 2015 16:27:08 -0700 (PDT) } 17, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) } 17, 40 -- by smtp.googlemail.com with ESMTPSA id } e6sm14989821qga.14.2015.10.06.16.27.07 } 17, 40 -- for {microscopy-at-microscopy.com} } 17, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM- } SHA256 bits=128/128); } 17, 40 -- Tue, 06 Oct 2015 16:27:07 -0700 (PDT) } 17, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} } 17, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply } 17, 40 -- {microscopylistserver-noreply-at-microscopy.com} } 17, 40 -- Subject: viaWWW:SEM of leather, thanks for feedback } 17, 40 -- References: {201510061758.t96Hw72s015940-at-ns.microscopy.com} } 17, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 17, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 17, 40 -- X-Forwarded-Message-Id: } {201510061758.t96Hw72s015940-at-ns.microscopy.com} } 17, 40 -- Message-ID: {561458CB.7070000-at-microscopy.com} } 17, 40 -- Date: Tue, 6 Oct 2015 18:27:07 -0500 } 17, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) } 17, 40 -- Gecko/20100101 Thunderbird/38.2.0 } 17, 40 -- MIME-Version: 1.0 } 17, 40 -- In-Reply-To: {201510061758.t96Hw72s015940-at-ns.microscopy.com} } 17, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 17, 40 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 44 -- From milesd-at-us.ibm.com Tue Oct 6 19:42:37 2015 9, 44 -- Received: from e19.ny.us.ibm.com (e19.ny.us.ibm.com [129.33.205.209]) 9, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t970gbko000954 9, 44 -- for {Microscopy-at-Microscopy.Com} ; Tue, 6 Oct 2015 19:42:37 -0500 9, 44 -- Received: from localhost 9, 44 -- by e19.ny.us.ibm.com with IBM ESMTP SMTP Gateway: Authorized Use Only! Violators will be prosecuted 9, 44 -- for {Microscopy-at-Microscopy.Com} from {milesd-at-us.ibm.com} ; 9, 44 -- Tue, 6 Oct 2015 20:42:34 -0400 9, 44 -- Received: from d01dlp03.pok.ibm.com (9.56.250.168) 9, 44 -- by e19.ny.us.ibm.com (146.89.104.206) with IBM ESMTP SMTP Gateway: Authorized Use Only! Violators will be prosecuted; 9, 44 -- Tue, 6 Oct 2015 20:42:33 -0400 9, 44 -- X-IBM-Helo: d01dlp03.pok.ibm.com 9, 44 -- X-IBM-MailFrom: milesd-at-us.ibm.com 9, 44 -- X-IBM-RcptTo: Microscopy-at-Microscopy.Com 9, 44 -- Received: from b01cxnp22034.gho.pok.ibm.com (b01cxnp22034.gho.pok.ibm.com [9.57.198.24]) 9, 44 -- by d01dlp03.pok.ibm.com (Postfix) with ESMTP id E11CDC9003C 9, 44 -- for {Microscopy-at-Microscopy.Com} ; Tue, 6 Oct 2015 20:30:44 -0400 (EDT) 9, 44 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 9, 44 -- by b01cxnp22034.gho.pok.ibm.com (8.14.9/8.14.9/NCO v10.0) with ESMTP id t970gXc754067246 9, 44 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Oct 2015 00:42:33 GMT 9, 44 -- Received: from d01av01.pok.ibm.com (localhost [127.0.0.1]) 9, 44 -- by d01av01.pok.ibm.com (8.14.4/8.14.4/NCO v10.0 AVout) with ESMTP id t970gWtw029146 9, 44 -- for {Microscopy-at-Microscopy.Com} ; Tue, 6 Oct 2015 20:42:32 -0400 9, 44 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.63.8.33]) 9, 44 -- by d01av01.pok.ibm.com (8.14.4/8.14.4/NCO v10.0 AVin) with ESMTP id t970gWRG029097 9, 44 -- for {Microscopy-at-Microscopy.Com} ; Tue, 6 Oct 2015 20:42:32 -0400 9, 44 -- In-Reply-To: {201510062327.t96NRq2E025082-at-ns.microscopy.com} 9, 44 -- References: {201510062327.t96NRq2E025082-at-ns.microscopy.com} 9, 44 -- To: Microscopy-at-Microscopy.Com 9, 44 -- MIME-Version: 1.0 9, 44 -- Subject: Re: [Microscopy] viaWWW:SEM of leather, thanks for feedback 9, 44 -- X-KeepSent: C4C970FE:E027641A-85257ED7:0002F9E8; 9, 44 -- type=4; name=$KeepSent 9, 44 -- X-Mailer: IBM Notes Release 9.0.1FP2 SHF37 August 25, 2014 9, 44 -- Message-ID: {OFC4C970FE.E027641A-ON85257ED7.0002F9E8-85257ED7.0003E584-at-us.ibm.com} 9, 44 -- From: Darrell Miles {milesd-at-us.ibm.com} 9, 44 -- Date: Tue, 6 Oct 2015 20:42:33 -0400 9, 44 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 9.0.1FP4|June 07, 2015) at 9, 44 -- 10/06/2015 20:42:34, 9, 44 -- Serialize complete at 10/06/2015 20:42:34 9, 44 -- Content-Type: text/plain; charset="US-ASCII" 9, 44 -- X-TM-AS-MML: disable 9, 44 -- X-Content-Scanned: Fidelis XPS MAILER 9, 44 -- x-cbid: 15100700-0057-0000-0000-000001CF8C8B ==============================End of - Headers==============================
Sounds as if you are trying, but if the charge is worse with dropping to 5kV that tells me you have lost the BSE contribution, this will be a big problem whist using a TTL system. The converted BSE provide SE, but because they are derived from BSE they do not carry so much charge information
The approach to a problem specimen should be as follows -
1. Use the smallest possible piece of material, fixed to the stub with a good quality conducting media. 2. Start at the lowest kV that you are able to work with, and a small spot size; all aimed at not damaging or charging the specimen. If you use a TTL system go to the standard detector in the lower chamber, with about 7mm working distance. You need a contribution of converted BSE (less charge) which you are unlikely to have with TTL detection. If the signal is too low, slightly increase the spot size. 3. If the specimen is not giving you a problem other than resolution, increase the magnification using a fast scan if possible, to reduce the intensity of beam on a particular area. 4. Having increased the magnification, as resolution is the problem, increase the kV slightly (e.g. if you were using 1kV go to 1.5kV). 5. As long as there is no charge continue stepping up the kV until you are happy with the result or the specimen charges. If you charge the specimen take it to air to discharge it, and go back to an earlier kV. Slight charge may be reduced by reducing the spot size slightly. 6. If resolution is still the problem, try moving the specimen closer to the final lens by a few mm, not too close as you will lose the very valuable converted BSE. 7. Still a resolution problem, if you have a TTL system try 10mm WD then in steps down to 5mm WD with this system, and see if you are able to obtain a satisfactory result (with many TTL systems between 7mm and 5mm works). 8. All of the time you are looking for a mix of kV, spot size, and WD that provides the best mix of signal and therefore performance.
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 07 October 2015 00:29 To: protrain-at-emcourses.com
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Case Western Reserve University
Title-Subject: [Filtered] Free workshop - 2 & 3 ‐ Dimensional Characterization of Soft and Porous Materials using DualBeam FIB ‐ SEM
Message: SCSAM will be hosting later this month a workshop on the use of FIB and SEM technology for soft materials.
"2&3‐Dimensional Characterization of Soft and Porous Materials using Dual Beams FIB‐SEM" presented by Brandon Van Leer (FEI)
Tuesday,Oct20,2015 Case Western Reserve University, White building room 411 12:30‐13:00ÂRefreshments 13:00‐14:00ÂPresentation ‐ Polymers 14:00‐15:00ÂPresentation ‐ Bio-materials
Focused ion beam (FIB) microscopy uses either a Ga+ or Xe+ ions to remove material from a sample for a variety of imaging and preparation techniques including cross-sections and thin sections for S/TEM analysis. Considerable work has examined the effects of FIB for sputtering and exposure on a number of hard materials such as metals or semiconductors. Often methods used to sputter hard materials do not necessarily translate well when working with soft materials such as polymers, tissues, porous materials or other beam sensitive materials. In this workshop, we will work with soft materials in the DualBeam FIB-SEM to optimize the sputter conditions to achieve optimal results for 2D and 3D characterization. Particularly, we will emphasize beam conditions for successful sputtering of soft materials, the use of cryo-stages for cryo DualBeam and investigate how to best optimize electron beam imaging conditions to mitigate charge or achieve the highest resolution.
More info online at http://www.msneo.org/uploads/3/1/7/7/31779867/2015_oct_-_fei__2d___3d_imaging_of_soft_material_workshop_2.pdf
To register for this workshop, Please email skd4-at-case.edu Use ÂSoft‐Materials as the subject line.
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We have a Bio-Rad Radiance 2100 Confocal Microscope that we no longer use. The confocal is attached to an upright Nikon microscope. We need to keep the Nikon microscope, but to give away the laser scanning system, including the scanhead, a blue diode laser (405 nm), an argon ion laser (457, 477, 488, 514 nm), a HeNe Laser (543 nm and a Red diode laser (637 nm). Please contact me if you are interested (it might be good for parts). It is FREE, but you do need to pay for all necessary shipping.
Thank you,
Zhaojie
Zhaojie Zhang, Ph.D. Director, Jenkins Microscopy Facility University of Wyoming PHONE: 307-766-3038 Email: zzhang-at-uwyo.edu
==============================Original Headers============================== 6, 50 -- From ZZhang-at-uwyo.edu Wed Oct 7 14:08:47 2015 6, 50 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1bon0118.outbound.protection.outlook.com [157.56.111.118]) 6, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t97J8lmk014849 6, 50 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2015 14:08:47 -0500 6, 50 -- Received: from SN1PR05MB1981.namprd05.prod.outlook.com (10.162.132.23) by 6, 50 -- SN1PR05MB1952.namprd05.prod.outlook.com (10.162.132.18) with Microsoft SMTP 6, 50 -- Server (TLS) id 15.1.293.16; Wed, 7 Oct 2015 19:08:44 +0000 6, 50 -- Received: from SN1PR05MB1981.namprd05.prod.outlook.com (10.162.132.23) by 6, 50 -- SN1PR05MB1981.namprd05.prod.outlook.com (10.162.132.23) with Microsoft SMTP 6, 50 -- Server (TLS) id 15.1.293.16; Wed, 7 Oct 2015 19:08:43 +0000 6, 50 -- Received: from SN1PR05MB1981.namprd05.prod.outlook.com ([10.162.132.23]) by 6, 50 -- SN1PR05MB1981.namprd05.prod.outlook.com ([10.162.132.23]) with mapi id 6, 50 -- 15.01.0293.007; Wed, 7 Oct 2015 19:08:43 +0000 6, 50 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 6, 50 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 50 -- Subject: Bio-Rad Radiance 2100 Confocal system give away 6, 50 -- Thread-Topic: Bio-Rad Radiance 2100 Confocal system give away 6, 50 -- Thread-Index: AdEBM19hhMevJ3mcQ223IfriWXWx2w== 6, 50 -- Date: Wed, 7 Oct 2015 19:08:43 +0000 6, 50 -- Message-ID: {SN1PR05MB1981F3BF9815DDFA5E1C8F87B4360-at-SN1PR05MB1981.namprd05.prod.outlook.com} 6, 50 -- Accept-Language: en-US 6, 50 -- Content-Language: en-US 6, 50 -- X-MS-Has-Attach: 6, 50 -- X-MS-TNEF-Correlator: 6, 50 -- authentication-results: spf=none (sender IP is ) 6, 50 -- smtp.mailfrom=ZZhang-at-uwyo.edu; 6, 50 -- x-originating-ip: [129.72.155.134] 6, 50 -- x-microsoft-exchange-diagnostics: 1;SN1PR05MB1981;5:yOusTJhSKkUofVCRbdRRgkU0tmYg8bwNkrnmVNV9IduYDNqnJ0rORVjLvoPlOZLoxfAyJys73tj11DsQ2L4/vqNoq81PyZqTQavhC4sT0dsd0HrPRO3YfZ4enItj6FPQDCRlEv5A0IZJG13ElOjMDQ==;24:FrdvOz4GI4tqjrUuwVHJHz60mJv0TvOLfbj9peD8yRrBInQZpfuxmqn4Nea9aWpwW9rL3U5w9IQnYsRFdqrV6myjVXTUNnoaDibt2z3tmi8=;20:CarRfyKQy10MYS03VLMM6XkDWlLtYsIVZo2JLMtgAEinpI4xDMKtnIHkDY/6foMtV63VzEinnzVZ5nCy3oUIkA== 6, 50 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:SN1PR05MB1981; 6, 50 -- x-microsoft-antispam-prvs: {SN1PR05MB19815BB8B3C334F03CC5B465B4360-at-SN1PR05MB1981.namprd05.prod.outlook.com} 6, 50 -- x-exchange-antispam-report-test: UriScan:; 6, 50 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(520078)(8121501046)(5005006)(3002001);SRVR:SN1PR05MB1981;BCL:0;PCL:0;RULEID:;SRVR:SN1PR05MB1981; 6, 50 -- x-forefront-prvs: 0722981D2A 6, 50 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(189002)(53754006)(252514010)(19580395003)(81156007)(80792005)(5008740100001)(102836002)(450100001)(86362001)(64706001)(74316001)(66066001)(97736004)(40100003)(11100500001)(5890100001)(101416001)(92566002)(88552001)(77096005)(107886002)(99286002)(2501003)(54356999)(33656002)(5002640100001)(50986999)(189998001)(105586002)(110136002)(5001960100002)(76576001)(5003600100002)(87936001)(5007970100001)(19580405001)(106356001)(229853001)(2351001)(5004730100002)(10400500002)(75432002)(2900100001)(90282001)(46102003)(122556002)(89122001);DIR:OUT;SFP:1102;SCL:1;SRVR:SN1PR05MB1981;H:SN1PR05MB1981.namprd05.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 6, 50 -- received-spf: None (protection.outlook.com: uwyo.edu does not designate 6, 50 -- permitted sender hosts) 6, 50 -- spamdiagnosticoutput: 1:23 6, 50 -- spamdiagnosticmetadata: NSPM 6, 50 -- Content-Type: text/plain; charset="us-ascii" 6, 50 -- MIME-Version: 1.0 6, 50 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 07 Oct 2015 19:08:43.4156 6, 50 -- (UTC) 6, 50 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 6, 50 -- X-MS-Exchange-CrossTenant-id: f9cdd7ad-825d-4601-8e9c-a325e02d52da 6, 50 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: SN1PR05MB1981 6, 50 -- X-Microsoft-Exchange-Diagnostics: 6, 50 -- 1;SN1PR05MB1952;2:3gMBDmsQLLcNAL7U6XMHwXVLlFzrcZo+w8Y9f4XktN3vRTjhaHMnp+Alr4QJzpzFfScf2dTK9FV0CFRcevRkHW7k3sPMjywtpSbo1DS0Jtqvyq64K4cFC5MTc7RQkjYsPIeWFTO07shXoa3ZWXqS/r9F0NlRWuc32NCGP+JalLA=;23:G7RoLQ+vtcC9XMQGBzRSmbeIw8arG1kwpRvDCxXMeLbYfDxMVbkBYzHz8aDflgO2Jtw7lFQ1PKU29ddanCu81I0Kr97xP34/cJa7U8xhQocbKwctuS++7Gi1iyp1c7fi7dazoIMpKn7zybMgs7gkqeh5xShBwOeGJGXN2Aw4dhml+Mh43EKlZyubNti6quqC 6, 50 -- X-OriginatorOrg: uwyo.edu 6, 50 -- Content-Transfer-Encoding: 8bit 6, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t97J8lmk014849 ==============================End of - Headers==============================
I agree with what Steve had to say and have some additional comments.
-- A sample of a square centimeter or so is probably not too large. The conductive media should make a continuous electrical connection between the bare metal stub and the upper surface and continuing onto the upper surface of the sample. I often use Leit C carbon adhesive from my friendly microscopy supply house because it combines electrical conductivity with excellent mechanical strength that materials like colloidal graphite (DAG) and silver paint don't have. Silver paint can also be problematic in EDS analysis.
-- The power that field emission SEM offers is incredibly bright source combined with a stable low voltage beam. Secondary electron imaging (SEI) at low voltage provides the best topographical analysis of surface roughness. Charge control in SEI is achieved by balancing the number of incident electrons entering the sample and electrons leaving as secondary and backscattered electrons. I highly recommend the book "Scanning Electron Microscopy and X-ray Microanalysis", 3rd Edition by Goldstein et al., Springer, 2013. The chapter on Procedures for Elimination of Charging in Nonconducting Specimens should be very helpful.
-- My 25 years of experience in low voltage, field emission SEM of polymers leads me to believe that some of the imaging problems experienced with leather may be similar to those observed in polymers. Both are insulators and consist of low atomic weight elements. Like Steve, I suggest you start at around 1 kV accelerating voltage. Use the spot size that the service engineers use to check your spatial resolution at 1 kV: this spot size is a good place to start because the manufacturer knows it gives an excellence balance of resolution and signal-to-noise.
-- For best results, I suggest you work with an uncoated sample. You might start with a coated sample then move to the bare, uncoated sample. Gold and gold-palladium coatings will obscure possibly important surface information.
-- Faster scan rates produce less charging than longer scan rates. If a single scan allows you to control charging but is somewhat noisy, average several scans recorded using shorter scan rates. Do a few quick tests on fresh areas of the sample to see how long a scan rate you can use without producing charging. Be aware that your default image acquisition scan rate may be much longer than the ideal scan rate for your sample. If this happens, record your images at the preferred rate.
-- Fortunately, sub-micrometer surface texture and porosity (as I would expect to see in collage-based leather surfaces) dissipate charging thus making imaging easier.
Sounds as if you are trying, but if the charge is worse with dropping to 5kV that tells me you have lost the BSE contribution, this will be a big problem whist using a TTL system. The converted BSE provide SE, but because they are derived from BSE they do not carry so much charge information
The approach to a problem specimen should be as follows -
1. Use the smallest possible piece of material, fixed to the stub with a good quality conducting media. 2. Start at the lowest kV that you are able to work with, and a small spot size; all aimed at not damaging or charging the specimen. If you use a TTL system go to the standard detector in the lower chamber, with about 7mm working distance. You need a contribution of converted BSE (less charge) which you are unlikely to have with TTL detection. If the signal is too low, slightly increase the spot size. 3. If the specimen is not giving you a problem other than resolution, increase the magnification using a fast scan if possible, to reduce the intensity of beam on a particular area. 4. Having increased the magnification, as resolution is the problem, increase the kV slightly (e.g. if you were using 1kV go to 1.5kV). 5. As long as there is no charge continue stepping up the kV until you are happy with the result or the specimen charges. If you charge the specimen take it to air to discharge it, and go back to an earlier kV. Slight charge may be reduced by reducing the spot size slightly. 6. If resolution is still the problem, try moving the specimen closer to the final lens by a few mm, not too close as you will lose the very valuable converted BSE. 7. Still a resolution problem, if you have a TTL system try 10mm WD then in steps down to 5mm WD with this system, and see if you are able to obtain a satisfactory result (with many TTL systems between 7mm and 5mm works). 8. All of the time you are looking for a mix of kV, spot size, and WD that provides the best mix of signal and therefore performance.
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 07 October 2015 00:29 To: protrain-at-emcourses.com
Thank you all for your interests in the confocal system. A new home for the system is found.
Best wishes,
Zhaojie
--------------------------------------------------------------------------------------- Hi All:
We have a Bio-Rad Radiance 2100 Confocal Microscope that we no longer use. The confocal is attached to an upright Nikon microscope. We need to keep the Nikon microscope, but to give away the laser scanning system, including the scanhead, a blue diode laser (405 nm), an argon ion laser (457, 477, 488, 514 nm), a HeNe Laser (543 nm and a Red diode laser (637 nm). Please contact me if you are interested (it might be good for parts). It is FREE, but you do need to pay for all necessary shipping.
Thank you,
Zhaojie
Zhaojie Zhang, Ph.D. Director, Jenkins Microscopy Facility University of Wyoming PHONE: 307-766-3038 Email: zzhang-at-uwyo.edu
==============================Original Headers============================== 6, 50 -- From ZZhang-at-uwyo.edu Wed Oct 7 14:08:47 2015 6, 50 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1bon0118.outbound.protection.outlook.com [157.56.111.118]) 6, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t97J8lmk014849 6, 50 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2015 14:08:47 -0500 6, 50 -- Received: from SN1PR05MB1981.namprd05.prod.outlook.com (10.162.132.23) by 6, 50 -- SN1PR05MB1952.namprd05.prod.outlook.com (10.162.132.18) with Microsoft SMTP 6, 50 -- Server (TLS) id 15.1.293.16; Wed, 7 Oct 2015 19:08:44 +0000 6, 50 -- Received: from SN1PR05MB1981.namprd05.prod.outlook.com (10.162.132.23) by 6, 50 -- SN1PR05MB1981.namprd05.prod.outlook.com (10.162.132.23) with Microsoft SMTP 6, 50 -- Server (TLS) id 15.1.293.16; Wed, 7 Oct 2015 19:08:43 +0000 6, 50 -- Received: from SN1PR05MB1981.namprd05.prod.outlook.com ([10.162.132.23]) by 6, 50 -- SN1PR05MB1981.namprd05.prod.outlook.com ([10.162.132.23]) with mapi id 6, 50 -- 15.01.0293.007; Wed, 7 Oct 2015 19:08:43 +0000 6, 50 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 6, 50 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 50 -- Subject: Bio-Rad Radiance 2100 Confocal system give away 6, 50 -- Thread-Topic: Bio-Rad Radiance 2100 Confocal system give away 6, 50 -- Thread-Index: AdEBM19hhMevJ3mcQ223IfriWXWx2w== 6, 50 -- Date: Wed, 7 Oct 2015 19:08:43 +0000 6, 50 -- Message-ID: {SN1PR05MB1981F3BF9815DDFA5E1C8F87B4360-at-SN1PR05MB1981.namprd05.prod.outlook.com} 6, 50 -- Accept-Language: en-US 6, 50 -- Content-Language: en-US 6, 50 -- X-MS-Has-Attach: 6, 50 -- X-MS-TNEF-Correlator: 6, 50 -- authentication-results: spf=none (sender IP is ) 6, 50 -- smtp.mailfrom=ZZhang-at-uwyo.edu; 6, 50 -- x-originating-ip: [129.72.155.134] 6, 50 -- x-microsoft-exchange-diagnostics: 1;SN1PR05MB1981;5:yOusTJhSKkUofVCRbdRRgkU0tmYg8bwNkrnmVNV9IduYDNqnJ0rORVjLvoPlOZLoxfAyJys73tj11DsQ2L4/vqNoq81PyZqTQavhC4sT0dsd0HrPRO3YfZ4enItj6FPQDCRlEv5A0IZJG13ElOjMDQ==;24:FrdvOz4GI4tqjrUuwVHJHz60mJv0TvOLfbj9peD8yRrBInQZpfuxmqn4Nea9aWpwW9rL3U5w9IQnYsRFdqrV6myjVXTUNnoaDibt2z3tmi8=;20:CarRfyKQy10MYS03VLMM6XkDWlLtYsIVZo2JLMtgAEinpI4xDMKtnIHkDY/6foMtV63VzEinnzVZ5nCy3oUIkA== 6, 50 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:SN1PR05MB1981; 6, 50 -- x-microsoft-antispam-prvs: {SN1PR05MB19815BB8B3C334F03CC5B465B4360-at-SN1PR05MB1981.namprd05.prod.outlook.com} 6, 50 -- x-exchange-antispam-report-test: UriScan:; 6, 50 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(520078)(8121501046)(5005006)(3002001);SRVR:SN1PR05MB1981;BCL:0;PCL:0;RULEID:;SRVR:SN1PR05MB1981; 6, 50 -- x-forefront-prvs: 0722981D2A 6, 50 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(189002)(53754006)(252514010)(19580395003)(81156007)(80792005)(5008740100001)(102836002)(450100001)(86362001)(64706001)(74316001)(66066001)(97736004)(40100003)(11100500001)(5890100001)(101416001)(92566002)(88552001)(77096005)(107886002)(99286002)(2501003)(54356999)(33656002)(5002640100001)(50986999)(189998001)(105586002)(110136002)(5001960100002)(76576001)(5003600100002)(87936001)(5007970100001)(19580405001)(106356001)(229853001)(2351001)(5004730100002)(10400500002)(75432002)(2900100001)(90282001)(46102003)(122556002)(89122001);DIR:OUT;SFP:1102;SCL:1;SRVR:SN1PR05MB1981;H:SN1PR05MB1981.namprd05.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 6, 50 -- received-spf: None (protection.outlook.com: uwyo.edu does not designate 6, 50 -- permitted sender hosts) 6, 50 -- spamdiagnosticoutput: 1:23 6, 50 -- spamdiagnosticmetadata: NSPM 6, 50 -- Content-Type: text/plain; charset="us-ascii" 6, 50 -- MIME-Version: 1.0 6, 50 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 07 Oct 2015 19:08:43.4156 6, 50 -- (UTC) 6, 50 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 6, 50 -- X-MS-Exchange-CrossTenant-id: f9cdd7ad-825d-4601-8e9c-a325e02d52da 6, 50 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: SN1PR05MB1981 6, 50 -- X-Microsoft-Exchange-Diagnostics: 6, 50 -- 1;SN1PR05MB1952;2:3gMBDmsQLLcNAL7U6XMHwXVLlFzrcZo+w8Y9f4XktN3vRTjhaHMnp+Alr4QJzpzFfScf2dTK9FV0CFRcevRkHW7k3sPMjywtpSbo1DS0Jtqvyq64K4cFC5MTc7RQkjYsPIeWFTO07shXoa3ZWXqS/r9F0NlRWuc32NCGP+JalLA=;23:G7RoLQ+vtcC9XMQGBzRSmbeIw8arG1kwpRvDCxXMeLbYfDxMVbkBYzHz8aDflgO2Jtw7lFQ1PKU29ddanCu81I0Kr97xP34/cJa7U8xhQocbKwctuS++7Gi1iyp1c7fi7dazoIMpKn7zybMgs7gkqeh5xShBwOeGJGXN2Aw4dhml+Mh43EKlZyubNti6quqC 6, 50 -- X-OriginatorOrg: uwyo.edu 6, 50 -- Content-Transfer-Encoding: 8bit 6, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t97J8lmk014849 ==============================End of - Headers==============================
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] TEM, SAED, tilting & Camera Constant
Message: Greetings,
We have a Mineralogy Grad student doing SAED on single mineral grains and is collecting patterns while tilting the sample holder [X-tilt only between 0-20 degrees]. The student is careful and thorough and is worried that the tilt will change her camera constant.
Question: to what degree does stage tilt affect the camera constant, and is there a recognized way to compensate for the change?
Any assistance, suggestion, or reference is appreciated.
Thanks, Tom
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Question: to what degree does stage tilt affect the camera constant, and is there a recognized way to compensate for the change?
Whenever you move to a new location or tilt the sample for acquisition of SAED patterns, you should recheck that the sample is still at eucentric height, and adjust if necessary. If lens conditions are kept the same and the sample is at eucentric height, the camera constant should be valid.
Best regards,
Elaine
Elaine F. Schumacher | Senior Research Scientist McCrone Associates, Inc. . 850 Pasquinelli Drive . Westmont, IL 60559 P. 630.887.7100 | F. 630.887.7417 | www.mccrone.com ISO/IEC 17025:2005 A2LA Accredited, Certificate #3631.01 . FDA/GDUFA/DEA Registered This email and any files attached may contain information that is legally privileged and/or confidential. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, printing, or copying of this communication is strictly prohibited without our permission.
==============================Original Headers============================== 9, 43 -- From eschumacher-at-mccrone.com Thu Oct 8 08:30:48 2015 9, 43 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 9, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t98DUm88006217 9, 43 -- for {Microscopy-at-Microscopy.com} ; Thu, 8 Oct 2015 08:30:48 -0500 9, 43 -- X-ASG-Debug-ID: 1444311045-07bbd4087f122fd0001-FOsErg 9, 43 -- Received: from TMGEX1.tmg.mccrone.com ([192.168.101.73]) by spam.mccrone.com with ESMTP id eMceAVNexcZVIshK for {Microscopy-at-Microscopy.com} ; Thu, 08 Oct 2015 08:30:45 -0500 (CDT) 9, 43 -- X-Barracuda-Envelope-From: eschumacher-at-mccrone.com 9, 43 -- Received: from TMGEX2.tmg.mccrone.com (192.168.101.74) by 9, 43 -- TMGEX1.tmg.mccrone.com (192.168.101.73) with Microsoft SMTP Server (TLS) id 9, 43 -- 15.0.913.22; Thu, 8 Oct 2015 08:30:44 -0500 9, 43 -- Received: from TMGEX2.tmg.mccrone.com ([::1]) by TMGEX2.tmg.mccrone.com 9, 43 -- ([fe80::ac43:5f4:37be:d22%14]) with mapi id 15.00.0913.011; Thu, 8 Oct 2015 9, 43 -- 08:30:44 -0500 9, 43 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 9, 43 -- To: "'Microscopy-at-Microscopy.com'" {Microscopy-at-Microscopy.com} 9, 43 -- Subject: Re: TEM, SAED, tilting & Camera Constant 9, 43 -- Thread-Topic: Re: TEM, SAED, tilting & Camera Constant 9, 43 -- X-ASG-Orig-Subj: Re: TEM, SAED, tilting & Camera Constant 9, 43 -- Thread-Index: AdEBzYDJ8YW68Zz0Tp2M5RuZO6Qp3Q== 9, 43 -- Date: Thu, 8 Oct 2015 13:30:44 +0000 9, 43 -- Message-ID: {d9ac44313e99447db49c907bb3790bd3-at-TMGEX2.tmg.mccrone.com} 9, 43 -- Accept-Language: en-US 9, 43 -- Content-Language: en-US 9, 43 -- X-MS-Has-Attach: 9, 43 -- X-MS-TNEF-Correlator: 9, 43 -- x-vipre-scanned: 0FCBCE5800AD580FCBCFA5 9, 43 -- x-originating-ip: [192.168.101.95] 9, 43 -- Content-Type: text/plain; charset="iso-8859-1" 9, 43 -- MIME-Version: 1.0 9, 43 -- X-Barracuda-Connect: UNKNOWN[192.168.101.73] 9, 43 -- X-Barracuda-Start-Time: 1444311045 9, 43 -- X-Barracuda-URL: https://spam.mccrone.com:443/cgi-mod/mark.cgi 9, 43 -- X-Virus-Scanned: by bsmtpd at mccrone.com 9, 43 -- X-Barracuda-BRTS-Status: 1 9, 43 -- X-Barracuda-Spam-Score: 0.60 9, 43 -- X-Barracuda-Spam-Status: No, SCORE=0.60 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests=COMMA_SUBJECT 9, 43 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.23303 9, 43 -- Rule breakdown below 9, 43 -- pts rule name description 9, 43 -- ---- ---------------------- -------------------------------------------------- 9, 43 -- 0.60 COMMA_SUBJECT Subject is like 'Re: FDSDS, this is a subject' 9, 43 -- Content-Transfer-Encoding: 8bit 9, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t98DUm88006217 ==============================End of - Headers==============================
From ferguson651651615e-at-gmail.com Thu Oct 8 09:41:24 2015 Return-Path: {ferguson651651615e-at-gmail.com} Received: from gmail.com ([121.78.112.104]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t98EfKxq029246 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 8 Oct 2015 09:41:22 -0500 Message-ID: {045CE8B3.1FB11E31-at-gmail.com}
X-from: erin.stempinski-at-nih.gov
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We are trying to do post-embed immunolabeling of cell pellets in Unicryl for a client. However, we are having difficulties sectioning her blocks. The blocks are extremely hydrophilic; even if the boat is very under-filled, water is still attracted to the block face and pulled over the knife. Any sections that we get are pretty much unusable. I have experience sectioning K4M, LR White, and Unicryl before, and the hydrophilicity has never been this bad.
The protocol we used is as follows:  Fixation in 4% PFA and 0.1% glutaraldehyde in phosphate buffer  Rinse in phosphate buffer (3 x 15 minutes)  Dehydration series of 25%, 50%, 75%, 95%, and 3 100% changes with a freshly opened bottle of EtOH  Infiltration of 1:3, 1:1, 3:1 of 100% EtOH and Unicryl (EtOH was same freshly opened bottle) and 4 changes of 100% Unicryl (2nd one was overnight)  Polymerization in Eppendorf tubes in a 55°C oven for 1 day, then a second day once we started having sectioning issues
Some troubleshooting we have done at the microtome includes:  Varying cutting speed and microtome arm cycle speed  Varying thickness setting of microtome arm advancement  Using a new knife and new section of the knife edge  Varying water level from slightly under-filled to severely under-filled  Varying block face size & shape
Any advice would be greatly appreciated.
P.S. I am on the listserv so replies to microscopy-at-microscopy.com are welcome.
Best,
Erin Stempinski [C] Electron Microscopy Technician NHLBI Electron Microscopy Core Facility MSC5570
erin.stempinski-at-nih.gov
301-496-6448
National Institutes of Health Building 14E Room 104A 14 Service Road West Bethesda, MD 20892-5570
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Erin, I have also used K4M and HM20, one being hydrophilic and later hydrophobic. I have switched to HM20 for the same problems you cite. I would eventually get sections with K4M, but not without a lot of duress. I wonder why you polymerized in an Eppendorf and not BEEM capsules? The container must be air tight and I know some Eppendorf's can leak. Also make sure you are adding enough catalyst, by weight not volume as viscous liquids will stick to any pipettes etc. Also I believe the K4M can be polymerized with up to 5% by weight water, so I am curious as to why you are going through 100% ethanol? I can typically stop at 90% ethanol and mix (i.e. LR White) as my first infiltration step, especially since your protocol does not protect membranes (you can use tannic acid and en-bloc UA staining for that). If the second day of curing helped your problem then its a polymerization issue. Hopefully all will work out, let us know.
Sincerely, Michael Delannoy
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X-from: erin.stempinski-at-nih.gov
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We are trying to do post-embed immunolabeling of cell pellets in Unicryl for a client. However, we are having difficulties sectioning her blocks. The blocks are extremely hydrophilic; even if the boat is very under-filled, water is still attracted to the block face and pulled over the knife. Any sections that we get are pretty much unusable. I have experience sectioning K4M, LR White, and Unicryl before, and the hydrophilicity has never been this bad.
The protocol we used is as follows:  Fixation in 4% PFA and 0.1% glutaraldehyde in phosphate buffer  Rinse in phosphate buffer (3 x 15 minutes)  Dehydration series of 25%, 50%, 75%, 95%, and 3 100% changes with a freshly opened bottle of EtOH  Infiltration of 1:3, 1:1, 3:1 of 100% EtOH and Unicryl (EtOH was same freshly opened bottle) and 4 changes of 100% Unicryl (2nd one was overnight)  Polymerization in Eppendorf tubes in a 55°C oven for 1 day, then a second day once we started having sectioning issues
Some troubleshooting we have done at the microtome includes:  Varying cutting speed and microtome arm cycle speed  Varying thickness setting of microtome arm advancement  Using a new knife and new section of the knife edge  Varying water level from slightly under-filled to severely under-filled  Varying block face size & shape
Any advice would be greatly appreciated.
P.S. I am on the listserv so replies to microscopy-at-microscopy.com are welcome.
Best,
Erin Stempinski [C] Electron Microscopy Technician NHLBI Electron Microscopy Core Facility MSC5570
erin.stempinski-at-nih.gov
301-496-6448
National Institutes of Health Building 14E Room 104A 14 Service Road West Bethesda, MD 20892-5570
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==============================Original Headers============================== 31, 24 -- From prvs=71639c1f5=mdelann1-at-jhmi.edu Thu Oct 8 12:15:11 2015 31, 24 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.200]) 31, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t98HFAlW025301 31, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Oct 2015 12:15:10 -0500 31, 24 -- X-IronPort-AV: E=Sophos;i="5.17,655,1437451200"; 31, 24 -- d="scan'208";a="97962588" 31, 24 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96]) 31, 24 -- by IPEB3.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 08 Oct 2015 13:14:56 -0400 31, 24 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 31, 24 -- To: {microscopy.listserver-at-gmail.com} 31, 24 -- Cc: {Microscopy-at-microscopy.com} , {microscopy-at-msa.microscopy.com} 31, 24 -- References: {201510081614.t98GEIwZ016484-at-ns.microscopy.com} 31, 24 -- In-Reply-To: {201510081614.t98GEIwZ016484-at-ns.microscopy.com} 31, 24 -- Subject: RE: [Microscopy] viaWWW:Unicryl sectioning issues 31, 24 -- Date: Thu, 8 Oct 2015 13:14:53 -0400 31, 24 -- Message-ID: {000301d101ec$da020490$8e060db0$-at-jhmi.edu} 31, 24 -- MIME-Version: 1.0 31, 24 -- Content-Type: text/plain; 31, 24 -- charset="iso-8859-1" 31, 24 -- X-Mailer: Microsoft Outlook 14.0 31, 24 -- Thread-Index: AQI16HkkPLU86rapzEjWTtemuzYThp2YBD6A 31, 24 -- Content-Language: en-us 31, 24 -- Content-Transfer-Encoding: 8bit 31, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t98HFAlW025301 ==============================End of - Headers==============================
From advertise.bz222uba-at-gmail.com Thu Oct 8 16:51:04 2015 Return-Path: {advertise.bz222uba-at-gmail.com} Received: from gmail.com ([211.58.174.237]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t98Lp10u018693 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 8 Oct 2015 16:51:02 -0500 Message-ID: {90F87DBD.83FC8DDE-at-gmail.com}
Hi Tom
The problem, as you rightly understand, is that a focal length change in the objective lens will change the camera constant. There are two ways to correct this type of problem - either set the eucentric point very accurately prior to tilting, or always focus the tilted specimen by using the eucentric height control (Z'). Either method is acceptable, but I would use the latter in preference.
Enjoy
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 08 October 2015 13:09 To: protrain-at-emcourses.com
I've already received several responses and I can't thank everyone enough for your help. Here's some additional information:
-Dehydration steps were 15 minutes with 95% overnight. We dehydrated to 100% for two reasons: 1. we have used this protocol with success previously for this target and 2. ultrastructural preservation/sectioning was really poor in past experience when doing post-embed immunolabeling with plants and LR White if the sample was not completely dehydrated.
-We used the Eppendorf tubes because the pellets were very small and delicate (one was just a small smear of cells along the side of the tube) and we were worried about losing them in the transfer to gelatin capsules. The sheet on Unicryl on EMS seems to indicate that they should polymerize in the Eppendorf tubes just fine. Is that not what other people are experiencing?
-We are currently polymerizing even more by UV at 4 degrees.
-We also got a suggestion to place the samples overnight in Dri-rite which we will also try.
Thanks again for your help. I'll keep everyone updated if something works!
Erin Stempinski [C] Electron Microscopy Technician NHLBI Electron Microscopy Core Facility MSC5570
erin.stempinski-at-nih.gov
301-496-6448
National Institutes of Health Building 14E Room 104A 14 Service Road West Bethesda, MD 20892-5570
-----Original Message----- X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu] Sent: Thursday, October 08, 2015 1:41 PM To: Stempinski, Erin (NIH/NHLBI) [C]
Erin, I have also used K4M and HM20, one being hydrophilic and later hydrophobic. I have switched to HM20 for the same problems you cite. I would eventually get sections with K4M, but not without a lot of duress. I wonder why you polymerized in an Eppendorf and not BEEM capsules? The container must be air tight and I know some Eppendorf's can leak. Also make sure you are adding enough catalyst, by weight not volume as viscous liquids will stick to any pipettes etc. Also I believe the K4M can be polymerized with up to 5% by weight water, so I am curious as to why you are going through 100% ethanol? I can typically stop at 90% ethanol and mix (i.e. LR White) as my first infiltration step, especially since your protocol does not protect membranes (you can use tannic acid and en-bloc UA staining for that). If the second day of curing helped your problem then it's a polymerization issue. Hopefully all will work out, let us know.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, October 08, 2015 12:14 PM To: mdelann1-at-jhmi.edu
X-from: erin.stempinski-at-nih.gov
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both erin.stempinski-at-nih.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
We are trying to do post-embed immunolabeling of cell pellets in Unicryl for a client. However, we are having difficulties sectioning her blocks. The blocks are extremely hydrophilic; even if the boat is very under-filled, water is still attracted to the block face and pulled over the knife. Any sections that we get are pretty much unusable. I have experience sectioning K4M, LR White, and Unicryl before, and the hydrophilicity has never been this bad.
The protocol we used is as follows: Â. Fixation in 4% PFA and 0.1% glutaraldehyde in phosphate buffer Â. Rinse in phosphate buffer (3 x 15 minutes) Â. Dehydration series of 25%, 50%, 75%, 95%, and 3 100% changes with a freshly opened bottle of EtOH Â. Infiltration of 1:3, 1:1, 3:1 of 100% EtOH and Unicryl (EtOH was same freshly opened bottle) and 4 changes of 100% Unicryl (2nd one was overnight) Â. Polymerization in Eppendorf tubes in a 55°C oven for 1 day, then a second day once we started having sectioning issues
Some troubleshooting we have done at the microtome includes: Â. Varying cutting speed and microtome arm cycle speed Â. Varying thickness setting of microtome arm advancement Â. Using a new knife and new section of the knife edge Â. Varying water level from slightly under-filled to severely under-filled Â. Varying block face size & shape
Any advice would be greatly appreciated.
P.S. I am on the listserv so replies to microscopy-at-microscopy.com are welcome.
Best,
Erin Stempinski [C] Electron Microscopy Technician NHLBI Electron Microscopy Core Facility MSC5570
erin.stempinski-at-nih.gov
301-496-6448
National Institutes of Health Building 14E Room 104A 14 Service Road West Bethesda, MD 20892-5570
Login Host: 128.231.237.4 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
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============================================
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==============================Original Headers============================== 31, 24 -- From prvs=71639c1f5=mdelann1-at-jhmi.edu Thu Oct 8 12:15:11 2015 31, 24 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.200]) 31, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t98HFAlW025301 31, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Oct 2015 12:15:10 -0500 31, 24 -- X-IronPort-AV: E=Sophos;i="5.17,655,1437451200"; 31, 24 -- d="scan'208";a="97962588" 31, 24 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96]) 31, 24 -- by IPEB3.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 08 Oct 2015 13:14:56 -0400 31, 24 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 31, 24 -- To: {microscopy.listserver-at-gmail.com} 31, 24 -- Cc: {Microscopy-at-microscopy.com} , {microscopy-at-msa.microscopy.com} 31, 24 -- References: {201510081614.t98GEIwZ016484-at-ns.microscopy.com} 31, 24 -- In-Reply-To: {201510081614.t98GEIwZ016484-at-ns.microscopy.com} 31, 24 -- Subject: RE: [Microscopy] viaWWW:Unicryl sectioning issues 31, 24 -- Date: Thu, 8 Oct 2015 13:14:53 -0400 31, 24 -- Message-ID: {000301d101ec$da020490$8e060db0$-at-jhmi.edu} 31, 24 -- MIME-Version: 1.0 31, 24 -- Content-Type: text/plain; 31, 24 -- charset="iso-8859-1" 31, 24 -- X-Mailer: Microsoft Outlook 14.0 31, 24 -- Thread-Index: AQI16HkkPLU86rapzEjWTtemuzYThp2YBD6A 31, 24 -- Content-Language: en-us 31, 24 -- Content-Transfer-Encoding: 8bit 31, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t98HFAlW025301 ==============================End of - Headers==============================
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From cheap.profile.backlinks-at-gmail.com Fri Oct 9 12:51:28 2015 Return-Path: {cheap.profile.backlinks-at-gmail.com} Received: from gmail.com ([123.1.180.75]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t99Hoxd7012183 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 9 Oct 2015 12:51:06 -0500 Reply-To: cheap.profile.backlinks-at-gmail.com
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Email: md-at-personifysearch.com Name: Mary Dickson
Message: We have recently had a world leading microscopy client open up several confocal opportunities throughout the US. The ideal candidate would have an expertise in confocal microscopy and a comfort working directly with customers.
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Mon Oct 12 08:28:32 2015 14, 40 -- Received: from mail-qk0-f176.google.com (mail-qk0-f176.google.com [209.85.220.176]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9CDSVYP014944 14, 40 -- for {microscopy-at-microscopy.com} ; Mon, 12 Oct 2015 08:28:32 -0500 14, 40 -- Received: by qkdo1 with SMTP id o1so48942549qkd.1 14, 40 -- for {microscopy-at-microscopy.com} ; Mon, 12 Oct 2015 06:28:29 -0700 (PDT) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=HBIpbYSLDZv/2JB3oAmKHf0rRpBtcHhxn9LWiqzMBIg=; 14, 40 -- b=bLIFU/2lLNna2rKNw1hNrF18ZbOVawOtKmD1/J5Mm1Pc/1q4kPX/0gR9/Fp6BXV8HT 14, 40 -- chqsT/6ozLvxxvcc7cD019tPVm8F3vNhEzdgQaVqqHFOpbb630jgyjZCJIG3/aLyKqOk 14, 40 -- +igf+KfUJJvSgLF8//m9ILCZDo0i6TAnaO3+dCpUigZL6PhPaDz17hgVGuJHYlRG5zUC 14, 40 -- lt7i/6IS20vlM8askAjTdBhXpnocVSL0MqYhQpRGZXp12i7W8prEh+JhD3gdkvoroPgx 14, 40 -- Z50JvHrPE4Pf1lRfkqFhxqKhbuNIhxW1FiDFxRWsWKQTQe5ktD5GsO7JkDjCeq7+4WK/ 14, 40 -- Bu1A== 14, 40 -- X-Received: by 10.55.24.193 with SMTP id 62mr14617114qky.24.1444656509040; 14, 40 -- Mon, 12 Oct 2015 06:28:29 -0700 (PDT) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id d62sm7074888qhc.19.2015.10.12.06.28.28 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 40 -- Mon, 12 Oct 2015 06:28:28 -0700 (PDT) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:Confocal Microscopy Job Opportunities 14, 40 -- References: {201510121239.t9CCdsU1011788-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201510121239.t9CCdsU1011788-at-ns.microscopy.com} 14, 40 -- Message-ID: {561BB57A.3060607-at-microscopy.com} 14, 40 -- Date: Mon, 12 Oct 2015 08:28:26 -0500 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.3.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201510121239.t9CCdsU1011788-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: scott.payne-at-ndsu.edu Name: Scott Payne
Organization: Electron Microscopy Core Facility North Dakota State University
Title-Subject: [Filtered] TEM: Electron dose rate estimation for a JEOL 210 LaB6 TEM
Message: Hi Everyone,
We have JEOL 2100 LaB6 TEM and would like to estimate the Âelectron dose samples are receiving during EELS acquisitions. So I went to the literature and found that for the most part, the beam current density at the sample was estimated from the read-out current density on the viewing screen without a specimen and the fluence was reported as e/nm^2.
} From a reference paper a JEOL 2010F was used to give .24 A/cm^2 and .62 A/cm^2 current densities (dose rates) with the actual dose (depending on the time) being in the x10^5 to 10^7 e/nm^2 range.
I thought, I can do the math . The read-out current density on the 2100 LaB6 is listed in pA/cm^2 units, so I converted to A/cm^2 to A/nm^2 and finally to e/nm^2/s for the dose rate and then multiplied by the exposure time to give an estimate for the electron dose However, values calculated this way are not believable (x10^-5 to 10^-8 range)..
A representative read-current density value for a 10 nm probe in the LaB6 is 80 pA/cm^2. Is the 2010F read-out current density that much greater (in A/cm^2)because of the field emission source? Or am I missing some crucial part of the calculation?
Thank you ..
Scott
Scott A. Payne Assistant Director Electron Microscopy Core Facility North Dakota State University Phone: 701.231.8234
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From ohn.bullocknewsqukwu-at-gmail.com Mon Oct 12 20:58:53 2015 Return-Path: {ohn.bullocknewsqukwu-at-gmail.com} Received: from gmail.com ([1.236.84.191]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id t9D1woJv003972 for {microscopylistserverarchive5-at-microscopy.com} ; Mon, 12 Oct 2015 20:58:52 -0500 Message-ID: {4A36492E.B7D9C338-at-gmail.com}
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Organization: Pathology West ICPMR, Westmead, NSW, Australia
Title-Subject: [Filtered] Asking for thoughts on Benchtop TEMs
Message: Does anyone in the life science and / or diagnostic pathology EM microscopy community have experience with Benchtop TEMs (e.g. LVEM25)?
I have been asked to consider how appropiate a benchtop TEM would be to diagnostic pathology.
All thoughts and experiences are welcome.
Levina
Levina Dear
levina.dear-at-health.nsw.gov.au
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==============================Original Headers============================== 22, 40 -- From microscopy.listserver-at-gmail.com Wed Oct 14 07:13:22 2015 22, 40 -- Received: from mail-qg0-f51.google.com (mail-qg0-f51.google.com [209.85.192.51]) 22, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9ECDLN2008777 22, 40 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2015 07:13:21 -0500 22, 40 -- Received: by qgx61 with SMTP id 61so40198114qgx.3 22, 40 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2015 05:13:18 -0700 (PDT) 22, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 22, 40 -- d=gmail.com; s=20120113; 22, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 22, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 22, 40 -- bh=8mNfwH2pW6IWU/dF58YRcSFBunxRUMb+lHW/KweM/2I=; 22, 40 -- b=n301GtA7I25/hLgKV1MoI5/ODYuBbbQGnRiXZ08GtKDld5IYg0Z5I66db7ly7J5ppS 22, 40 -- bym6HIZTI+yPiewcKSpnDQf2RSJaBiubWJJyEi+z79HyMXQZ0lDJr9eoce002P/B53b8 22, 40 -- en8Ops9AwLporv4iO3W16lAuW5+5S5IAy5Xa8qpVT7kTlhUY8H7Q9wASut/X5AewGrb3 22, 40 -- wq10DtOEKvUoYIhh0pfNol8V9BnvUWwZiXkKcBNeykZo6rVXVutdX1pfX9W70OFPtyrY 22, 40 -- PokoYQAjjcJrpRhWXqmTG05ijap2KeKjiUp7ixvVKwJhXqqcpv1D/Up+L2deuVWqgjxy 22, 40 -- g3VQ== 22, 40 -- X-Received: by 10.140.86.42 with SMTP id o39mr3377053qgd.102.1444824798709; 22, 40 -- Wed, 14 Oct 2015 05:13:18 -0700 (PDT) 22, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 22, 40 -- by smtp.googlemail.com with ESMTPSA id 79sm3218273qhw.4.2015.10.14.05.13.18 22, 40 -- for {microscopy-at-microscopy.com} 22, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 22, 40 -- Wed, 14 Oct 2015 05:13:18 -0700 (PDT) 22, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 22, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 22, 40 -- {microscopylistserver-noreply-at-microscopy.com} 22, 40 -- Subject: viaWWW:Asking for thoughts on Benchtop TEMs 22, 40 -- References: {201510140114.t9E1EmNV014469-at-ns.microscopy.com} 22, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 22, 40 -- X-Forwarded-Message-Id: {201510140114.t9E1EmNV014469-at-ns.microscopy.com} 22, 40 -- Message-ID: {561E46DD.7080301-at-microscopy.com} 22, 40 -- Date: Wed, 14 Oct 2015 07:13:17 -0500 22, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 22, 40 -- Gecko/20100101 Thunderbird/38.3.0 22, 40 -- MIME-Version: 1.0 22, 40 -- In-Reply-To: {201510140114.t9E1EmNV014469-at-ns.microscopy.com} 22, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 22, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
} On Oct 12, 2015, at 5:57 PM, microscopy.listserver-at-gmail.com wrote: } } Email: scott.payne-at-ndsu.edu } Name: Scott Payne } } Organization: Electron Microscopy Core Facility North Dakota State University } } Title-Subject: [Filtered] TEM: Electron dose rate estimation for a JEOL 210 LaB6 TEM } } Message: Hi Everyone, } } We have JEOL 2100 LaB6 TEM and would like to estimate the Âelectron dose samples are receiving } during EELS acquisitions. So I went to the literature and found that for the most part, the beam } current density at the sample was estimated from the read-out current density on the viewing screen } without a specimen and the fluence was reported as e/nm^2. } } } From a reference paper a JEOL 2010F was used to give .24 A/cm^2 and .62 A/cm^2 current densities (dose rates) with the actual dose (depending on the time) being in the x10^5 to 10^7 e/nm^2 range. } } I thought, I can do the math } . The read-out current density on the 2100 LaB6 is listed in pA/cm^2 } units, so I converted to A/cm^2 to A/nm^2 and finally to e/nm^2/s for the dose rate and then } multiplied by the exposure time to give an estimate for the electron dose } However, values } calculated this way are not believable (x10^-5 to 10^-8 range).. } } A representative read-current density value for a 10 nm probe in the LaB6 is 80 pA/cm^2. Is the } 2010F read-out current density that much greater (in A/cm^2)because of the field emission source? Or } am I missing some crucial part of the calculation? } } } } Thank you } .. } } Scott } } Scott A. Payne } Assistant Director } Electron Microscopy Core Facility } North Dakota State University } Phone: 701.231.8234
Dear Scott, I plugged in numbers for a simpler situationâ1 A/cm^2âas follows: 1 A/cm^2=1 C/s*cm^2, and, since 1 C=6.241*10^18 e, and 1 cm=10^7 nm, 1A/cm^2=6.241*10^18*10^(-14)t=6.241*10^4t e/nm^2, where t is the exposure time in seconds, which is pretty much the same as the reference. Yours, Bill
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I worked in biological microscopy many years ago and in polymer microscopy for the last 33 years. TEM of conventional biological and polymer microscopy use heavy metal staining, i.e. osmium and ruthenium tetroxides in polymer microscopy and osmium tetroxide, uranyl acetate and lead citrate in biological microscopy. In all heavy metal stained samples (polymer and biological), amplitude scattering is the mechanism of imaging. I have used TEM at 100-200 kV in both disciplines with excellent results. More pertinent to your question, I have had good results using brightfield STEM imaging (STEM) at 30 kV in our field emission SEM (FESEM) using a brightfield transmitted electron detector located below the sample stage. The ~1.5 nm at 15 kV resolution of our Hitachi S-4300 FESEM/STEM is lower than is seen in conventional TEM's, and modern FESEM's and TEM/STEM's.
As one would expect, the contrast using FE-SEM/STEM is appreciably higher at 25 kV than at 100-200 kV. We found this an advantage in polymer microscopy. The lower spatial resolution was an issue at magnifications above ~50kx.
You would need to use fixed beam TEM, not STEM. The limiting resolution of STEM in a bench-top instrument will undoubtedly be poor compared to a much more sophisticated high voltage TEM/STEM.
Assuming that the spatial resolution of the bench-top TEM is adequate, my biggest concern is vibration. A good quality table with active an anti-vibration isolation will almost certainly be essential. Under no circumstances should you place this bench-top TEM on a standard lab bench that is shared with other folks in the lab. These benches make poor microscope tables under any circumstances. When shared with people doing other tasks, or even leaning against the bench discussing the latest football match, the vibration becomes prohibitive.
Finally, I would send several vendors a couple of samples of differing section thickness, possibly 70 nm and 100 nm (or thicker if you work in that range), for analysis useful magnifications. Have them send you TIF files of each sample; don't accept JPG or other compressed formats.
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Organization: Pathology West ICPMR, Westmead, NSW, Australia
Title-Subject: [Filtered] Asking for thoughts on Benchtop TEMs
Message: Does anyone in the life science and / or diagnostic pathology EM microscopy community have experience with Benchtop TEMs (e.g. LVEM25)?
I have been asked to consider how appropiate a benchtop TEM would be to diagnostic pathology.
All thoughts and experiences are welcome.
Levina
Levina Dear
levina.dear-at-health.nsw.gov.au
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==============================Original Headers============================== 22, 40 -- From microscopy.listserver-at-gmail.com Wed Oct 14 07:13:22 2015 22, 40 -- Received: from mail-qg0-f51.google.com (mail-qg0-f51.google.com [209.85.192.51]) 22, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9ECDLN2008777 22, 40 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2015 07:13:21 -0500 22, 40 -- Received: by qgx61 with SMTP id 61so40198114qgx.3 22, 40 -- for {microscopy-at-microscopy.com} ; Wed, 14 Oct 2015 05:13:18 -0700 (PDT) 22, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 22, 40 -- d=gmail.com; s=20120113; 22, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 22, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 22, 40 -- bh=8mNfwH2pW6IWU/dF58YRcSFBunxRUMb+lHW/KweM/2I=; 22, 40 -- b=n301GtA7I25/hLgKV1MoI5/ODYuBbbQGnRiXZ08GtKDld5IYg0Z5I66db7ly7J5ppS 22, 40 -- bym6HIZTI+yPiewcKSpnDQf2RSJaBiubWJJyEi+z79HyMXQZ0lDJr9eoce002P/B53b8 22, 40 -- en8Ops9AwLporv4iO3W16lAuW5+5S5IAy5Xa8qpVT7kTlhUY8H7Q9wASut/X5AewGrb3 22, 40 -- wq10DtOEKvUoYIhh0pfNol8V9BnvUWwZiXkKcBNeykZo6rVXVutdX1pfX9W70OFPtyrY 22, 40 -- PokoYQAjjcJrpRhWXqmTG05ijap2KeKjiUp7ixvVKwJhXqqcpv1D/Up+L2deuVWqgjxy 22, 40 -- g3VQ== 22, 40 -- X-Received: by 10.140.86.42 with SMTP id o39mr3377053qgd.102.1444824798709; 22, 40 -- Wed, 14 Oct 2015 05:13:18 -0700 (PDT) 22, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 22, 40 -- by smtp.googlemail.com with ESMTPSA id 79sm3218273qhw.4.2015.10.14.05.13.18 22, 40 -- for {microscopy-at-microscopy.com} 22, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 22, 40 -- Wed, 14 Oct 2015 05:13:18 -0700 (PDT) 22, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 22, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 22, 40 -- {microscopylistserver-noreply-at-microscopy.com} 22, 40 -- Subject: viaWWW:Asking for thoughts on Benchtop TEMs 22, 40 -- References: {201510140114.t9E1EmNV014469-at-ns.microscopy.com} 22, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 22, 40 -- X-Forwarded-Message-Id: {201510140114.t9E1EmNV014469-at-ns.microscopy.com} 22, 40 -- Message-ID: {561E46DD.7080301-at-microscopy.com} 22, 40 -- Date: Wed, 14 Oct 2015 07:13:17 -0500 22, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 22, 40 -- Gecko/20100101 Thunderbird/38.3.0 22, 40 -- MIME-Version: 1.0 22, 40 -- In-Reply-To: {201510140114.t9E1EmNV014469-at-ns.microscopy.com} 22, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 22, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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"An expert is a person who has made all the mistakes which can be made in a very narrow field.â and "Prediction is very difficult, especially if it's about the future." Niels Bohr
==============================Original Headers============================== 42, 31 -- From microscopy.gmb-at-gmail.com Fri Oct 16 11:55:17 2015 42, 31 -- Received: from mail-io0-f176.google.com (mail-io0-f176.google.com [209.85.223.176]) 42, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9GGtHek013890 42, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Oct 2015 11:55:17 -0500 42, 31 -- Received: by iow1 with SMTP id 1so131048293iow.1 42, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Oct 2015 09:55:14 -0700 (PDT) 42, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 42, 31 -- d=gmail.com; s=20120113; 42, 31 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 42, 31 -- :content-type:content-transfer-encoding; 42, 31 -- bh=+ZpoCAa9k6nYIph20ORUu0QWwGcbDozojxoBF10fWJY=; 42, 31 -- b=iuPr5OgyBFI2exZy0SbqOviD5OOhBOcIiq3v446ua3dC5QyhjK52XgJlFTrNIndFx3 42, 31 -- sX7cjKk9VhI+fgB0xVp7ySq9c4z9u08YCvfdUheF5X4twPcjFif2Nzt3vGBzCqY6sBMq 42, 31 -- Fuu83jHMNBuRX+Ax2x1SvolRDvPv8+hPY5PaC6C1n3VCPQ3dJg5NO/sfjIBLWs/ObQP8 42, 31 -- M/BJIjScVFOaYKXbD+Ovd4N3vM/KhYkGVxRl87rrWj6ZKW2d8WJ2lNHyRNIUH4WdhMSa 42, 31 -- pQImXOk9MGkGxgqEzBlKwSasA/GuAEuJqMp4MBuPsMGIVWCnLzWKDERchb4m16S+nWuw 42, 31 -- Grqg== 42, 31 -- MIME-Version: 1.0 42, 31 -- X-Received: by 10.107.164.224 with SMTP id d93mr15607623ioj.25.1445014514020; 42, 31 -- Fri, 16 Oct 2015 09:55:14 -0700 (PDT) 42, 31 -- Received: by 10.107.141.70 with HTTP; Fri, 16 Oct 2015 09:55:13 -0700 (PDT) 42, 31 -- In-Reply-To: {201510141242.t9ECgTB2027248-at-ns.microscopy.com} 42, 31 -- References: {201510141242.t9ECgTB2027248-at-ns.microscopy.com} 42, 31 -- Date: Fri, 16 Oct 2015 11:55:13 -0500 42, 31 -- Message-ID: {CADhGOTJ3-Ex_JoUzAG_38mdHVvsqjKHny82u7DETOdgsNiwk2A-at-mail.gmail.com} 42, 31 -- Subject: Fwd: [Microscopy] viaWWW:Asking for thoughts on Benchtop TEMs 42, 31 -- From: Gary Brown {microscopy.gmb-at-gmail.com} 42, 31 -- To: Listserver {Microscopy-at-microscopy.com} 42, 31 -- Content-Type: text/plain; charset=UTF-8 42, 31 -- Content-Transfer-Encoding: 8bit 42, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9GGtHek013890 ==============================End of - Headers==============================
Dear All, We have recently starting taking reservations for our atomic force microscopy training course in 2016. This course is in its fifth year. The course features a mix of hands-on classes with different AFMs, lectures of background and theory, some advanced applications talks from invited experts in AFM, and a guided data analysis class. The course will take place over four days (16th to 19th April, 2016), in the beautiful city of Porto, Portugal. The fee includes course materials (all lectures, and software on disk), a copy of the book Atomic Force Microscopy, written by myself, refreshments, and a coarse meal. Also the course is a way off, places are very limited, and we are already receiving reservations, so early reservation of a place is recommended. For more details go to http://afmhelp.com/course, or email me at afmhelp-at-gmail.com Please do pass on this information to anyone who may be interested. Best Regards, Pete. ____________________________________________________________________________ Atomic Force Microscopy Dr Peter Eaton and Dr Paul West Available through all good bookshops, or direct from Oxford University Press at: http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com
==============================Original Headers============================== 3, 19 -- From petereaton-at-hotmail.com Fri Oct 16 13:38:22 2015 3, 19 -- Received: from DUB004-OMC3S5.hotmail.com (dub004-omc3s5.hotmail.com [157.55.2.14]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9GIcMPT014462 3, 19 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2015 13:38:22 -0500 3, 19 -- Received: from DUB122-W43 ([157.55.2.7]) by DUB004-OMC3S5.hotmail.com over TLS secured channel with Microsoft SMTPSVC(7.5.7601.23008); 3, 19 -- Fri, 16 Oct 2015 11:38:19 -0700 3, 19 -- X-TMN: [IucIM63NSqwogXVcLClIXKxieCRJ86Ie] 3, 19 -- X-Originating-Email: [petereaton-at-hotmail.com] 3, 19 -- Message-ID: {DUB122-W438702B3BC9ECA0CB01AA8C83D0-at-phx.gbl} 3, 19 -- From: Peter Eaton {petereaton-at-hotmail.com} 3, 19 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 19 -- Subject: Porto AFM Training Course 2016 3, 19 -- Date: Fri, 16 Oct 2015 18:38:18 +0000 3, 19 -- Importance: Normal 3, 19 -- Content-Type: text/plain; charset="Windows-1252" 3, 19 -- MIME-Version: 1.0 3, 19 -- X-OriginalArrivalTime: 16 Oct 2015 18:38:19.0043 (UTC) FILETIME=[D3D6EF30:01D10841] 3, 19 -- Content-Transfer-Encoding: 8bit 3, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9GIcMPT014462 ==============================End of - Headers==============================
I need help finding a vendor. I need a low magnification objective for my Nikon Optiphot. I would like to have 1x if possible. The existing lenses are marked 210/0, meaning tube length 210 mm. Even if I can't match the tube length, I would like to try whatever objective I can find. Background information: I'm using an upright compound microscope (Nikon Optiphot) in bright field reflected light. I get clear images of the surfaces of interest, but I need a wider field of view than I have with my 5x objective. regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 2, 36 -- From donc-at-asmicro.com Fri Oct 16 15:01:46 2015 2, 36 -- Received: from resqmta-ch2-05v.sys.comcast.net (resqmta-ch2-05v.sys.comcast.net [69.252.207.37]) 2, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9GK1kxc009173 2, 36 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2015 15:01:46 -0500 2, 36 -- Received: from resomta-ch2-11v.sys.comcast.net ([69.252.207.107]) 2, 36 -- by resqmta-ch2-05v.sys.comcast.net with comcast 2, 36 -- id Vw1P1r0022Ka2Q501w1jMR; Fri, 16 Oct 2015 20:01:43 +0000 2, 36 -- Received: from asm20 ([68.58.83.137]) 2, 36 -- by resomta-ch2-11v.sys.comcast.net with comcast 2, 36 -- id Vw1i1r00J2xmMPG01w1in1; Fri, 16 Oct 2015 20:01:43 +0000 2, 36 -- Message-ID: {AA814F66CA104C689E21594DF4F78826-at-asm20} 2, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 2, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com} 2, 36 -- Subject: 1x objective for Nikon optiphot 2, 36 -- Date: Fri, 16 Oct 2015 16:01:47 -0400 2, 36 -- MIME-Version: 1.0 2, 36 -- Content-Type: text/plain; 2, 36 -- format=flowed; 2, 36 -- charset="iso-8859-1"; 2, 36 -- reply-type=original 2, 36 -- Content-Transfer-Encoding: 7bit 2, 36 -- X-Priority: 3 2, 36 -- X-MSMail-Priority: Normal 2, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 2, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 2, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 2, 36 -- s=q20140121; t=1445025703; 2, 36 -- bh=JUlflr7F481GtM3n6QqWjDA8o3pM4mQq6kzrdXLDtFY=; 2, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version: 2, 36 -- Content-Type; 2, 36 -- b=gcsbfsIEBtKhHryS7mEYQZKZeqkd1WFCk+KYlAz5BV6ATy43c3EJ/psEyGFO5jBhc 2, 36 -- sEQKvTr4B9ScPz/st3b9GJORkHql+NU6ruY/xhSUimLUXO+dU/1gWnz5jO3q63dld8 2, 36 -- Cbkvli9p7s97pCfuhmNPKqCFEybIECaHp7wBrnCgGInjULbJAq5SQaSWk1r73SVgrC 2, 36 -- SX1vu3KehnXekbe9I55edowpFcV4Vr/3Xl3UZexkObPhrX5rbCoVfU2O03aA2HKjkL 2, 36 -- yNNKwmuxHDxZcNSwdNU5oBdN4QgNA0QulOBgrRh/30JekD+TPuMvUsRvT/vEk2fneW 2, 36 -- 91Sz9QDoyVBgg== ==============================End of - Headers==============================
From news3409384-at-gmail.com Sat Oct 17 08:01:24 2015 Return-Path: {news3409384-at-gmail.com} Received: from gmail.com ([211.192.178.225]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9HD1KRa026825 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 17 Oct 2015 08:01:22 -0500 Reply-To: news3409384-at-gmail.com
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X-from: m.arredondo-at-qub.ac.uk
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Title-Subject: [Filtered] Research Fellow position available
Message: 3 years research fellow opening at Queen's University Belfast: "Far-From-Equilibrium Processing of Ferroelectric Thin Films on Glass and Polymeric Substrates"
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==============================Original Headers============================== 13, 40 -- From microscopy.listserver-at-gmail.com Tue Oct 20 07:42:13 2015 13, 40 -- Received: from mail-qg0-f47.google.com (mail-qg0-f47.google.com [209.85.192.47]) 13, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9KCgCaM016392 13, 40 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2015 07:42:13 -0500 13, 40 -- Received: by qgad10 with SMTP id d10so13140060qga.3 13, 40 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2015 05:42:09 -0700 (PDT) 13, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 40 -- d=gmail.com; s=20120113; 13, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 13, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 13, 40 -- bh=OTzjoL4PLO/aZICuHq5lTd2DVCug/KRdSouoh3Zt5VE=; 13, 40 -- b=XRu+4ZNfGNPa81dHW5Oy/Bt85OMsFRDJbkn5vqzZac9RtCHjpYTf7asDpNtFx5QO+H 13, 40 -- FW+KZRSPJ6X+FnysMa7SqwS//gd4/fIqrRSR+Bl+10COEwKVToCLzihNEv+/nYwb1t7q 13, 40 -- LvXWqUNlkmZ+MvXzz5v5BwtAHUyJGtE8B44g/Q57CNb5lip2dof+nPCjCRp62U+AmPke 13, 40 -- E7LxRheCzf6PJAxfzlSR1nqjAhdCmP8ocMJ+O6+/hnPbaZN29SYcXzimXQ6F74qWDDgZ 13, 40 -- /kAh8HPOsKVkiX/hRINUWc7TS7iqIgBGfzfIfzPZLx3gwuKJ4NxIGf4gEw0REGNKZ47/ 13, 40 -- /ZYw== 13, 40 -- X-Received: by 10.140.134.211 with SMTP id 202mr3750694qhg.51.1445344928961; 13, 40 -- Tue, 20 Oct 2015 05:42:08 -0700 (PDT) 13, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 13, 40 -- by smtp.googlemail.com with ESMTPSA id 18sm241736qgx.47.2015.10.20.05.42.08 13, 40 -- for {microscopy-at-microscopy.com} 13, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 40 -- Tue, 20 Oct 2015 05:42:08 -0700 (PDT) 13, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 13, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 13, 40 -- {microscopylistserver-noreply-at-microscopy.com} 13, 40 -- Subject: viaWWW:Research Fellow position available 13, 40 -- References: {201510200745.t9K7j3NQ011710-at-ns.microscopy.com} 13, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 40 -- X-Forwarded-Message-Id: {201510200745.t9K7j3NQ011710-at-ns.microscopy.com} 13, 40 -- Message-ID: {5626369F.9030900-at-microscopy.com} 13, 40 -- Date: Tue, 20 Oct 2015 07:42:07 -0500 13, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 13, 40 -- Gecko/20100101 Thunderbird/38.3.0 13, 40 -- MIME-Version: 1.0 13, 40 -- In-Reply-To: {201510200745.t9K7j3NQ011710-at-ns.microscopy.com} 13, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The LeBeau research group in the Department of Materials Science & Engineering at North Carolina State University is seeking a postdoctoral research associate to start after December 2015. The post-doc will be responsible for the development of quantitative imaging and spectroscopy at the atomic scale using aberration corrected scanning transmission electron microscopy. Studies will include a wide range of materials, for example functional oxide-nitride interfaces and state-of-the-art alloys. These efforts will account for 70% of the post-docâs responsibilities.
The remaining 30% of the post-docs responsibilities will be to oversee training of students/researchers and research collaborations on the Titan microscope. The post-doc will train users to operate the microscopy safely and independently, will help develop facility policies for user training and oversight, and will perform periodic service work for internal and external clients. The post-doc will also contribute to developing the internal and external user bases by presenting highlights of research on the websites and throughout campus, by providing tours to potential users, and by giving presentations at national and international meetings.
NCSU has an aberration corrected FEI Titan G2 microscope for ultra-high resolution STEM imaging and chemical analysis. Other equipment includes a JEOL 2010 STEM/TEM, several conventional TEMs, and a FIB. The position requires a Ph. D. in materials science or a related field. Experience with aberration corrected STEM is desired. Duration is between 1-3 years and salary is commensurate with qualifications.
Interested candidates should apply online here:
http://jobs.ncsu.edu/postings/59664
AA/EEO. In addition, NC State welcomes all persons without regard to sexual orientation.
==============================Original Headers============================== 6, 34 -- From jmlebeau-at-gmail.com Tue Oct 20 08:51:17 2015 6, 34 -- Received: from mail-yk0-f176.google.com (mail-yk0-f176.google.com [209.85.160.176]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9KDpHj9007330 6, 34 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2015 08:51:17 -0500 6, 34 -- Received: by yknn9 with SMTP id n9so16813637ykn.0 6, 34 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2015 06:51:14 -0700 (PDT) 6, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 34 -- d=gmail.com; s=20120113; 6, 34 -- h=from:content-type:content-transfer-encoding:subject:message-id:date 6, 34 -- :to:mime-version; 6, 34 -- bh=Ye0MPJMMw88Mv6LOUF6zcwQI2sGZh/6y6xXlbtv6xi4=; 6, 34 -- b=RY84HhvBUHFlm0K1js/6m4QVqa7d5klU4LBIwNRpexkZQlJliD0lx9M6PlWXxtzofU 6, 34 -- TwCD22YIBPuxgaeHRL3oDM/sSNkfUNqFHl3Fxpldpx5CpFnLfTeZZy2biMnWEjoqyDj0 6, 34 -- M+mnmFFxXBM08lCRQduNbXOhuPLFX2tMUbJiPbNct4H7lZ/+Ryke8HN7Ejc9D8QfSlAG 6, 34 -- 9FylhpWpro84ssa6FdbeFRAAEzr2/eWValIXuh0ywkm8YZDstkhdUqzwmXoDIzVuIOn4 6, 34 -- HWUE0labmUs6dfdv3WK788AqqVLN0PC2taVLi6arTB4FNhyMq4pYDzg5sbfHsFcfcSmW 6, 34 -- +KiQ== 6, 34 -- X-Received: by 10.129.135.69 with SMTP id x66mr2380231ywf.209.1445349073947; 6, 34 -- Tue, 20 Oct 2015 06:51:13 -0700 (PDT) 6, 34 -- Received: from jmln-76-1.mse.ncsu.edu (jmln-76-1.mse.ncsu.edu. [152.14.71.198]) 6, 34 -- by smtp.gmail.com with ESMTPSA id g82sm1900783ywa.15.2015.10.20.06.51.13 6, 34 -- for {microscopy-at-microscopy.com} 6, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 6, 34 -- Tue, 20 Oct 2015 06:51:13 -0700 (PDT) 6, 34 -- From: James LeBeau {jmlebeau-at-gmail.com} 6, 34 -- Content-Type: text/plain; charset=utf-8 6, 34 -- Subject: STEM Postdoctoral Scholar Position 6, 34 -- Message-Id: {4A5D7D96-5E45-4F03-8C0E-48761E879848-at-gmail.com} 6, 34 -- Date: Tue, 20 Oct 2015 09:51:13 -0400 6, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 34 -- Mime-Version: 1.0 (Mac OS X Mail 9.0 \(3094\)) 6, 34 -- X-Mailer: Apple Mail (2.3094) 6, 34 -- Content-Transfer-Encoding: 8bit 6, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9KDpHj9007330 ==============================End of - Headers==============================
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Email: yisong_han-at-yahoo.co.uk Name: Yisong Han
Organization: Ulster University
Title-Subject: [Filtered] Lift-out tips for Kleindiek system
Message: Dear All,
There is a Kleindiek system (MM3A-EM) for the in-situ lift-out of TEM lamellae on our FEI Quanta 3D FIB/SEM. I am wondering if any of you could recommend some suppliers who sell the right type of tips/probe for such a system. Thanks very much in advance.
Yisong
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==============================Original Headers============================== 13, 40 -- From microscopy.listserver-at-gmail.com Tue Oct 20 18:44:51 2015 13, 40 -- Received: from mail-qk0-f172.google.com (mail-qk0-f172.google.com [209.85.220.172]) 13, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9KNipEE027009 13, 40 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2015 18:44:51 -0500 13, 40 -- Received: by qkca6 with SMTP id a6so15298499qkc.3 13, 40 -- for {microscopy-at-microscopy.com} ; Tue, 20 Oct 2015 16:44:47 -0700 (PDT) 13, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 40 -- d=gmail.com; s=20120113; 13, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 13, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 13, 40 -- bh=fIcIMO1idan6ko2SlatW0ERnmLK/jr4DN9OeKnxKe6M=; 13, 40 -- b=bFQRP5r9r09lLIIo+GJ7rmMdle53nICtkzshfZUBzm8yr3HKkZAWr6nWqaxSe7FHh2 13, 40 -- ocV9JKWGNFygI5XnE59V1MzpZvgF409et0A4h4GZ2nxTUV+oupf9pwv+4erl+xSp3TmH 13, 40 -- 3mQYWIEYwzxfxw+RaPp4BO+sLSFhAumxeyq0UJ33WQ/lWafaY6LtvENkgvh60v+gvxcG 13, 40 -- 8wd2t3lCw201ToTpSP8cuaU7OGUK/KIhQDUAfD8lSPgVOSZuaqftVUAyqk+gxu88hHQF 13, 40 -- PmcdWT5BdRszfxt0uax/3ejzUnlLyCAzPuOvrc/ovGBtRG8AnxNElh/EpPKH/CwBLZgd 13, 40 -- 4/uw== 13, 40 -- X-Received: by 10.55.22.162 with SMTP id 34mr7671330qkw.3.1445384687355; 13, 40 -- Tue, 20 Oct 2015 16:44:47 -0700 (PDT) 13, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 13, 40 -- by smtp.googlemail.com with ESMTPSA id s84sm2228061qki.14.2015.10.20.16.44.46 13, 40 -- for {microscopy-at-microscopy.com} 13, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 40 -- Tue, 20 Oct 2015 16:44:46 -0700 (PDT) 13, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 13, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 13, 40 -- {microscopylistserver-noreply-at-microscopy.com} 13, 40 -- Subject: viaWWW:Lift-out tips for Kleindiek 13, 40 -- References: {201510201328.t9KDSiQG006809-at-ns.microscopy.com} 13, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 40 -- X-Forwarded-Message-Id: {201510201328.t9KDSiQG006809-at-ns.microscopy.com} 13, 40 -- Message-ID: {5626D1ED.70607-at-microscopy.com} 13, 40 -- Date: Tue, 20 Oct 2015 18:44:45 -0500 13, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 13, 40 -- Gecko/20100101 Thunderbird/38.3.0 13, 40 -- MIME-Version: 1.0 13, 40 -- In-Reply-To: {201510201328.t9KDSiQG006809-at-ns.microscopy.com} 13, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Thanks to everyone who gave suggestions for our Unicryl sectioning issues. We were able to get sections after polymerizing with UV at 4 degrees for 48 hours. Here are some other suggestions we got for anyone else who runs into such an issue:
-Putting samples in dri-rite/silica gel/some other desiccant overnight -Curing samples for 1 week -Varying the knife angle -Adding EtOH or Tween if the knife allows to break the surface tension
Thanks again for all the help!
Erin Stempinski [C] Electron Microscopy Technician NHLBI Electron Microscopy Core Facility MSC5570
erin.stempinski-at-nih.gov
301-496-6448
National Institutes of Health Building 14E Room 104A 14 Service Road West Bethesda, MD 20892-5570
-----Original Message----- X-from: Stempinski, Erin (NIH/NHLBI) [C] Sent: Friday, October 09, 2015 9:01 AM To: Stempinski, Erin (NIH/NHLBI) [C]
I've already received several responses and I can't thank everyone enough for your help. Here's some additional information:
-Dehydration steps were 15 minutes with 95% overnight. We dehydrated to 100% for two reasons: 1. we have used this protocol with success previously for this target and 2. ultrastructural preservation/sectioning was really poor in past experience when doing post-embed immunolabeling with plants and LR White if the sample was not completely dehydrated.
-We used the Eppendorf tubes because the pellets were very small and delicate (one was just a small smear of cells along the side of the tube) and we were worried about losing them in the transfer to gelatin capsules. The sheet on Unicryl on EMS seems to indicate that they should polymerize in the Eppendorf tubes just fine. Is that not what other people are experiencing?
-We are currently polymerizing even more by UV at 4 degrees.
-We also got a suggestion to place the samples overnight in Dri-rite which we will also try.
Thanks again for your help. I'll keep everyone updated if something works!
Erin Stempinski [C] Electron Microscopy Technician NHLBI Electron Microscopy Core Facility MSC5570
erin.stempinski-at-nih.gov
301-496-6448
National Institutes of Health Building 14E Room 104A 14 Service Road West Bethesda, MD 20892-5570
We are trying to do post-embed immunolabeling of cell pellets in Unicryl for a client. However, we are having difficulties sectioning her blocks. The blocks are extremely hydrophilic; even if the boat is very under-filled, water is still attracted to the block face and pulled over the knife. Any sections that we get are pretty much unusable. I have experience sectioning K4M, LR White, and Unicryl before, and the hydrophilicity has never been this bad.
The protocol we used is as follows: Â. Fixation in 4% PFA and 0.1% glutaraldehyde in phosphate buffer Â. Rinse in phosphate buffer (3 x 15 minutes) Â. Dehydration series of 25%, 50%, 75%, 95%, and 3 100% changes with a freshly opened bottle of EtOH Â. Infiltration of 1:3, 1:1, 3:1 of 100% EtOH and Unicryl (EtOH was same freshly opened bottle) and 4 changes of 100% Unicryl (2nd one was overnight) Â. Polymerization in Eppendorf tubes in a 55°C oven for 1 day, then a second day once we started having sectioning issues
Some troubleshooting we have done at the microtome includes: Â. Varying cutting speed and microtome arm cycle speed Â. Varying thickness setting of microtome arm advancement Â. Using a new knife and new section of the knife edge Â. Varying water level from slightly under-filled to severely under-filled Â. Varying block face size & shape
Any advice would be greatly appreciated.
P.S. I am on the listserv so replies to microscopy-at-microscopy.com are welcome.
Best,
Erin Stempinski [C] Electron Microscopy Technician NHLBI Electron Microscopy Core Facility MSC5570
erin.stempinski-at-nih.gov
301-496-6448
National Institutes of Health Building 14E Room 104A 14 Service Road West Bethesda, MD 20892-5570
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From a.lesnes-at-wanadoo.fr Thu Oct 22 15:49:06 2015 Return-Path: {a.lesnes-at-wanadoo.fr} Received: from masscom.arvixevps.com (masscom.arvixevps.com [108.175.155.94]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9MKn6dl002645; Thu, 22 Oct 2015 15:49:06 -0500 Message-Id: {201510222049.t9MKn6dl002645-at-ns.microscopy.com} Received: from [5.170.60.204] (port=30731 helo=User) by masscom.arvixevps.com with esmtpa (Exim 4.86) (envelope-from {a.lesnes-at-wanadoo.fr} ) id 1ZpMn9-0004Hh-EM; Thu, 22 Oct 2015 13:48:55 -0700 Reply-To: {mrsgracef-at-aol.com}
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Title-Subject: [Filtered] scanning method for stone tools?
Message: Greetings!
I am an archaeologist specializing in experimental archaeology with hunting tools, and microwear on stone tools. I did an extensive project for a thesis with atlatl darts and arrows shot into a carcass, and then among other things, looked at the stone projectile points under a microscope. We used a differential-interference binocular microscope with polarized light and Nomarski optics. We took photographs at interesting locations, the most useful of which were recorded at 400X. This is not a new process, but we did find some new, previously unreported phenomena, and this can be attributed to the amount of detail recorded in the experiment.
The microwear study has taken a long time, and is lacking in several aspects. Specifically, future tests would benefit heavily from having a microscopic view of the entire surface of each point both before and after the experiment for direct comparison. Using our current approach this is impossible. My question is, is there a way to scan the face of a stone projectile point at 100-400X and end up with a digital recording of the microscopic surface? I strongly suspect there is, but I wonder if the imagery would be of the type that would be useful to us. This is an exciting and very new field for me, so I'm sure my question seems very simplistic. I would benefit from a little direction.
Thanks! Devin
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Fri Oct 23 07:32:47 2015 14, 40 -- Received: from mail-qk0-f170.google.com (mail-qk0-f170.google.com [209.85.220.170]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9NCWkjc027693 14, 40 -- for {microscopy-at-microscopy.com} ; Fri, 23 Oct 2015 07:32:47 -0500 14, 40 -- Received: by qkbl190 with SMTP id l190so72488912qkb.2 14, 40 -- for {microscopy-at-microscopy.com} ; Fri, 23 Oct 2015 05:32:43 -0700 (PDT) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=MinyTEJ1/4ogDUkBFOZnBtadIlN1No97Lap+d6uAqyk=; 14, 40 -- b=Zkf4Hr6mE5V9HPiEaYNPNhqhXNL73WegO4GVlbnx2UhKzu0/GRghUVlegOrYhX20G/ 14, 40 -- xBlOU4t7ef1rZScZI5hSQ1GTEMUjbCHQtag9HJHXcWikTL2JqkwltagFKB+m2UrdnJbJ 14, 40 -- E6IOq66UplQW1BoeWBWiSpnAs9P35kJGGFoHpLxL0Pe2OzpUyERiWyRG3NmZ8ttFQ6n+ 14, 40 -- ak9KbPay75wCvvi1FTKXLuiyLjq00s6GxxofHpoWHa/hN8xm0w2pZuiZ8b4fNuQ6yjFP 14, 40 -- qloKdlM8XU3h9cf7Vtua4gEC28HRDhiFaXWXJZLkQmNzu31sKaszkhRWa535nHYDbupG 14, 40 -- yXmw== 14, 40 -- X-Received: by 10.140.34.83 with SMTP id k77mr25059211qgk.23.1445603563026; 14, 40 -- Fri, 23 Oct 2015 05:32:43 -0700 (PDT) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id 68sm7347771qgy.16.2015.10.23.05.32.42 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 40 -- Fri, 23 Oct 2015 05:32:42 -0700 (PDT) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:scanning method for stone tools? 14, 40 -- References: {201510230052.t9N0q2ev011836-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201510230052.t9N0q2ev011836-at-ns.microscopy.com} 14, 40 -- Message-ID: {562A28E9.1050902-at-microscopy.com} 14, 40 -- Date: Fri, 23 Oct 2015 07:32:41 -0500 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.3.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201510230052.t9N0q2ev011836-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am not sure what do you mean under " a digital recording of the microscopic surface", but if it includes 3-D reconstruction, I'd recommend Micro-CT or newer optical systems with so-called infinite focus. Some confocal microscopes could be useful also (not really sure, I do not work with them). If you need just a digital picture, the scanning electron microscope (SEM) will be of use.
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, October 23, 2015 7:34 AM To: Dusevich, Vladimir
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Title-Subject: [Filtered] scanning method for stone tools?
Message: Greetings!
I am an archaeologist specializing in experimental archaeology with hunting tools, and microwear on stone tools. I did an extensive project for a thesis with atlatl darts and arrows shot into a carcass, and then among other things, looked at the stone projectile points under a microscope. We used a differential-interference binocular microscope with polarized light and Nomarski optics. We took photographs at interesting locations, the most useful of which were recorded at 400X. This is not a new process, but we did find some new, previously unreported phenomena, and this can be attributed to the amount of detail recorded in the experiment.
The microwear study has taken a long time, and is lacking in several aspects. Specifically, future tests would benefit heavily from having a microscopic view of the entire surface of each point both before and after the experiment for direct comparison. Using our current approach this is impossible. My question is, is there a way to scan the face of a stone projectile point at 100-400X and end up with a digital recording of the microscopic surface? I strongly suspect there is, but I wonder if the imagery would be of the type that would be useful to us. This is an exciting and very new field for me, so I'm sure my question seems very simplistic. I would benefit from a little direction.
Thanks! Devin
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Fri Oct 23 07:32:47 2015 14, 40 -- Received: from mail-qk0-f170.google.com (mail-qk0-f170.google.com [209.85.220.170]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9NCWkjc027693 14, 40 -- for {microscopy-at-microscopy.com} ; Fri, 23 Oct 2015 07:32:47 -0500 14, 40 -- Received: by qkbl190 with SMTP id l190so72488912qkb.2 14, 40 -- for {microscopy-at-microscopy.com} ; Fri, 23 Oct 2015 05:32:43 -0700 (PDT) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=MinyTEJ1/4ogDUkBFOZnBtadIlN1No97Lap+d6uAqyk=; 14, 40 -- b=Zkf4Hr6mE5V9HPiEaYNPNhqhXNL73WegO4GVlbnx2UhKzu0/GRghUVlegOrYhX20G/ 14, 40 -- xBlOU4t7ef1rZScZI5hSQ1GTEMUjbCHQtag9HJHXcWikTL2JqkwltagFKB+m2UrdnJbJ 14, 40 -- E6IOq66UplQW1BoeWBWiSpnAs9P35kJGGFoHpLxL0Pe2OzpUyERiWyRG3NmZ8ttFQ6n+ 14, 40 -- ak9KbPay75wCvvi1FTKXLuiyLjq00s6GxxofHpoWHa/hN8xm0w2pZuiZ8b4fNuQ6yjFP 14, 40 -- qloKdlM8XU3h9cf7Vtua4gEC28HRDhiFaXWXJZLkQmNzu31sKaszkhRWa535nHYDbupG 14, 40 -- yXmw== 14, 40 -- X-Received: by 10.140.34.83 with SMTP id k77mr25059211qgk.23.1445603563026; 14, 40 -- Fri, 23 Oct 2015 05:32:43 -0700 (PDT) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id 68sm7347771qgy.16.2015.10.23.05.32.42 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 40 -- Fri, 23 Oct 2015 05:32:42 -0700 (PDT) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:scanning method for stone tools? 14, 40 -- References: {201510230052.t9N0q2ev011836-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201510230052.t9N0q2ev011836-at-ns.microscopy.com} 14, 40 -- Message-ID: {562A28E9.1050902-at-microscopy.com} 14, 40 -- Date: Fri, 23 Oct 2015 07:32:41 -0500 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.3.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201510230052.t9N0q2ev011836-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 22, 30 -- From DusevichV-at-umkc.edu Fri Oct 23 14:35:07 2015 22, 30 -- Received: from mst-rip6-umkc-out.um.umsystem.edu (mst-rip6-umkc-out.um.umsystem.edu [198.209.50.152]) 22, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9NJZ7J6025463 22, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Oct 2015 14:35:07 -0500 22, 30 -- X-IronPort-Anti-Spam-Filtered: true 22, 30 -- X-IronPort-Anti-Spam-Result: A2AMAgC5iypW/9SeoM9bA4M2VG8GsV+MRwENgVkXCoV8AoFAOBQBAQEBAQEBfwuEMgEBAQQ6FDcEAgEIEQMBAQELAgoBBwkHIRABFAkIAgQKCQgVBId6AxINwTINRgGEDwEBAQEBAQEBAQEBAQEBAQEBAQEBARiGd4R+glANGgGBMhACAR8hEgUGDAEBghpPHYEUBYdAhVSEFgGEUwYnAYUYhT9Rg01Ig3eOQodNHwEBQoIRHYFVcgGEewcXI4EGAQEB 22, 30 -- X-IPAS-Result: A2AMAgC5iypW/9SeoM9bA4M2VG8GsV+MRwENgVkXCoV8AoFAOBQBAQEBAQEBfwuEMgEBAQQ6FDcEAgEIEQMBAQELAgoBBwkHIRABFAkIAgQKCQgVBId6AxINwTINRgGEDwEBAQEBAQEBAQEBAQEBAQEBAQEBARiGd4R+glANGgGBMhACAR8hEgUGDAEBghpPHYEUBYdAhVSEFgGEUwYnAYUYhT9Rg01Ig3eOQodNHwEBQoIRHYFVcgGEewcXI4EGAQEB 22, 30 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 22, 30 -- by mst-rip6-exch-relay.um.umsystem.edu with ESMTP; 23 Oct 2015 14:34:47 -0500 22, 30 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.171]) by 22, 30 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0248.002; 22, 30 -- Fri, 23 Oct 2015 14:34:47 -0500 22, 30 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 22, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 22, 30 -- Subject: RE: [Microscopy] viaWWW:scanning method for stone tools? 22, 30 -- Thread-Topic: [Microscopy] viaWWW:scanning method for stone tools? 22, 30 -- Thread-Index: AQHRDY8bQw096iOkg0efUcE3cxzolZ55d1/A 22, 30 -- Date: Fri, 23 Oct 2015 19:34:46 +0000 22, 30 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DC9E360-at-UM-MBX-T01.um.umsystem.edu} 22, 30 -- References: {201510231234.t9NCY6CV028323-at-ns.microscopy.com} 22, 30 -- In-Reply-To: {201510231234.t9NCY6CV028323-at-ns.microscopy.com} 22, 30 -- Accept-Language: en-US 22, 30 -- Content-Language: en-US 22, 30 -- X-MS-Has-Attach: 22, 30 -- X-MS-TNEF-Correlator: 22, 30 -- x-originating-ip: [134.193.157.109] 22, 30 -- Content-Type: text/plain; charset="us-ascii" 22, 30 -- MIME-Version: 1.0 22, 30 -- Content-Transfer-Encoding: 8bit 22, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9NJZ7J6025463 ==============================End of - Headers==============================
} On Oct 23, 2015, at 5:57 AM, microscopy.listserver-at-gmail.com wrote: } } Message: Greetings! } } I am an archaeologist specializing in experimental archaeology with hunting tools, and microwear on } stone tools. I did an extensive project for a thesis with atlatl darts and arrows shot into a } carcass, and then among other things, looked at the stone projectile points under a microscope. We } used a differential-interference binocular microscope with polarized light and Nomarski optics. We } took photographs at interesting locations, the most useful of which were recorded at 400X. This is } not a new process, but we did find some new, previously unreported phenomena, and this can be } attributed to the amount of detail recorded in the experiment. } } The microwear study has taken a long time, and is lacking in several aspects. Specifically, future } tests would benefit heavily from having a microscopic view of the entire surface of each point both } before and after the experiment for direct comparison. Using our current approach this is } impossible. My question is, is there a way to scan the face of a stone projectile point at 100-400X } and end up with a digital recording of the microscopic surface? I strongly suspect there is, but I } wonder if the imagery would be of the type that would be useful to us. This is an exciting and very } new field for me, so I'm sure my question seems very simplistic. I would benefit from a little } direction. } Hi Devin, As Vladimir said, SEM could be sufficient. If you can collect the secondary electron signal, it is sensitive to the orientation of the surface of the specimen. There are a few potential difficulties, however: I donât know what your specimen is made from, but if itâs something like obsidian, there could be charging issues. There could also be difficulties with field of view, depending on the size of the specimenâa few cm times 400X mag will give an image about a meter across. This can be constructed by montaging smaller images, but there may be very many of these. Neither of these is insurmountable, so talk to your local SEM guy or find a facility that will provide the service you needâthis list is a good place to start. Yours, Bill
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Hi,
why not try light microscopy? In many material labs there should be compound microscopes with epi-illumination of some sort. Also in some biomedical labs these machines are around. We have here used e.g. epi-darkfield for similar things. It will be in the magnification range you need, you can do extended depth of field projections if you're not getting the full sample focussed at once. With a motorised stage it is also easy to get a tiled image so you can image a very large specimen into one dataset. I guess the sample prep for light microscopy may be easier than for SEM? But my SEM experience is from last century ...
Best, Christian
On 23.10.2015 14:53, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: dpettig08-at-gmail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both dpettig08-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: dpettig08-at-gmail.com } Name: Devin Pettigrew } } Organization: University of Arkansas } } Title-Subject: [Filtered] scanning method for stone tools? } } Message: Greetings! } } I am an archaeologist specializing in experimental archaeology with hunting tools, and microwear on } stone tools. I did an extensive project for a thesis with atlatl darts and arrows shot into a } carcass, and then among other things, looked at the stone projectile points under a microscope. We } used a differential-interference binocular microscope with polarized light and Nomarski optics. We } took photographs at interesting locations, the most useful of which were recorded at 400X. This is } not a new process, but we did find some new, previously unreported phenomena, and this can be } attributed to the amount of detail recorded in the experiment. } } The microwear study has taken a long time, and is lacking in several aspects. Specifically, future } tests would benefit heavily from having a microscopic view of the entire surface of each point both } before and after the experiment for direct comparison. Using our current approach this is } impossible. My question is, is there a way to scan the face of a stone projectile point at 100-400X } and end up with a digital recording of the microscopic surface? I strongly suspect there is, but I } wonder if the imagery would be of the type that would be useful to us. This is an exciting and very } new field for me, so I'm sure my question seems very simplistic. I would benefit from a little } direction. } } Thanks! } Devin } } Login Host: 72.204.56.198 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
1. Given what little in the way of qualitative size range is suggested by your text (100-400x), your best bets would be Laser Profilometry or Scanning White Light Interference Microscopy (SWLIM).
These will give you a Z elevation per X,Y coordinate. You can then post-process to look at depth changes, orientation/directional-related aspects, fractal dimensions, etc.
For simple work an old light section or Schmaltz Profile Microscope (I have one at the office but use it very infrequently). This gives a simple x-y line and would need to be automated for crunching larger data. See Abouelatta, 3D surface roughness measurement using a light sectioning vision system, Proc WCE, 698-703, 2010.
Since you are using DIC, you could create a 3D profile using MATLAB processing on the image - but this assumes isotropic behavior which is probably not the case for you.
2. A couple of papers in my library that you should get:
Stemp, Laser profilometry and length-scale analysis of stone tools, second series experiment results, Scanning, 32, 4, 233-243, 2010 Borel, SEM and Optical Light Microscopy, two complementary approaches for the understanding and interp of usewear and residues on stone tools, J Arch Sci, 48, 46-59, 2014
Hint: You should do a lit search first.
3. I would also recommend getting Mahaney's "Atlas of Sand Grain Surface Textures and Applications" It is not the same media (mineral v tool) but the data on wear features observed in the SEM on sand grains will carry over to wear on tools (he has also done wear on teeth).
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==============================Original Headers============================== 16, 27 -- From ph2-at-sprynet.com Mon Oct 26 10:22:53 2015 16, 27 -- Received: from elasmtp-curtail.atl.sa.earthlink.net (elasmtp-curtail.atl.sa.earthlink.net [209.86.89.64]) 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9QFMr4u026112 16, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Oct 2015 10:22:53 -0500 16, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 16, 27 -- s=dk20050327; d=sprynet.com; 16, 27 -- b=RcoqoOPG9pyt9Ycv5mvtrb2KuvIgofjHuRqX3/vz5rIXo1AnoJm1RLnL/k11dn0Y; 16, 27 -- h=Received:From:To:Cc:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language:X-ELNK-Trace:X-Originating-IP; 16, 27 -- Received: from [68.58.112.163] (helo=AAHHPdv7) 16, 27 -- by elasmtp-curtail.atl.sa.earthlink.net with esmtpa (Exim 4.67) 16, 27 -- (envelope-from {ph2-at-sprynet.com} ) 16, 27 -- id 1ZqjbW-00067j-5C; Mon, 26 Oct 2015 11:22:34 -0400 16, 27 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 16, 27 -- To: {dpettig08-at-gmail.com} 16, 27 -- Cc: "Microscopy Listserve" {Microscopy-at-Microscopy.Com} 16, 27 -- Subject: Re: viaWWW:scanning method for stone tools? 16, 27 -- Date: Mon, 26 Oct 2015 11:22:32 -0400 16, 27 -- Message-ID: {007201d11002$2378e6c0$6a6ab440$-at-sprynet.com} 16, 27 -- MIME-Version: 1.0 16, 27 -- Content-Type: text/plain; 16, 27 -- charset="us-ascii" 16, 27 -- Content-Transfer-Encoding: 7bit 16, 27 -- X-Mailer: Microsoft Outlook 14.0 16, 27 -- Thread-Index: AdEP/jOzoSrR3t+VSCeWE/OuIuDuIw== 16, 27 -- Content-Language: en-us 16, 27 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f926101ea43a5bcc99276130d5f4e959cf350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 16, 27 -- X-Originating-IP: 68.58.112.163 ==============================End of - Headers==============================
The Electron Microscopy Service (EMS) of the Research Resources Center (RRC) at the University of Illinois at Chicago has an open position for a Microscopy Specialist. The facility provides electron and laser microscopy and surface analysis services for the university research community and external organizations from two sites on the campus. The open position is in the RRC-East facility, which specializes in physical and materials science fields. The east side facility includes a JEOL JEM-ARM200CF aberration corrected STEM, JEOL JEM-3010 and JEOL JEM-100CX TEMs, Kratos AXIS-165 XPS and a Renishaw Raman Spectrometer.
Qualified candidates must have a bachelorâs degree (preferably a masterâs degree or higher) in physical sciences, engineering or a related field, with at least three years materials science transmission electron microscopy experience. They will supervise the operation of the specimen preparation area, including record keeping and maintenance and will assist/supervise in the day to day running of the Electron Microscopes and Surface Analysis. Interpersonal/ Communications skills are important, as this individual will work with users, provide technical advice and demonstrate how microscopy can advance their research.
Further information may be obtained by consulting the following website: http://www.rrc.uic.edu/
Applicants should submit the following: cover letter, complete curriculum vitae, and the names and addresses of three references. For fullest consideration, applications should be received by November 18, 2015. All materials should be uploaded to https://jobs.uic.edu/job-board/job-details?jobID=57495
The University of Illinois at Chicago is an Equal Opportunity, Affirmative Action employer. Minorities, women, veterans and individuals with disabilities are encouraged to apply.
-- Alan W Nicholls, PhD Associate Director, RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110, Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Tel: 312 996 1227
Web site http://www.rrc.uic.edu/ems Wiki site https://wiki.rrc.uic.edu/wiki/EMS
==============================Original Headers============================== 13, 19 -- From nicholls-at-uic.edu Mon Oct 26 10:34:40 2015 13, 19 -- Received: from mail-5.cc.uic.edu (mail-5.cc.uic.edu [128.248.156.155]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9QFYeik005457 13, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Oct 2015 10:34:40 -0500 13, 19 -- Received: from [131.193.156.96] (131-193-156-96.pclabs.uic.edu [131.193.156.96]) 13, 19 -- (authenticated bits=0) 13, 19 -- by mail-5.cc.uic.edu (8.14.4/8.14.4) with ESMTP id t9QFYaNV012440 13, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT) 13, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Oct 2015 10:34:36 -0500 13, 19 -- To: Microscopy-at-microscopy.com 13, 19 -- From: Alan Nicholls {nicholls-at-uic.edu} 13, 19 -- Subject: TEM Position Available Core Facility 13, 19 -- Message-ID: {562E483A.6090608-at-uic.edu} 13, 19 -- Date: Mon, 26 Oct 2015 10:35:22 -0500 13, 19 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 13, 19 -- Thunderbird/38.3.0 13, 19 -- MIME-Version: 1.0 13, 19 -- Content-Type: text/plain; charset=utf-8; format=flowed 13, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We are looking for someone with electron microscopy experience to join our core facility at Purdue. We have TEM, SEM, cryo, EDX, dual-beam, immuno plus a wide range of sample prep equipment â HPF, ultramicrotomes, cryo sectioning and vibratome. If you have experience in any , some or all (really?) of the above we would be interested to hear from you. There is a formal job description and application procedure at http://purdue.taleo.net/careersection/wl/jobdetail.ftl?job=1502194&lang=en
Informal enquiries can be made to me.
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu
==============================Original Headers============================== 7, 30 -- From gilpin-at-purdue.edu Tue Oct 27 12:43:54 2015 7, 30 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9RHhrj4002867 7, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Oct 2015 12:43:53 -0500 7, 30 -- Received: from WPVEXCHUB03.purdue.lcl (wpvexchub03.itap.purdue.edu [172.30.136.67]) 7, 30 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id t9RHhn0x032226 7, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Oct 2015 13:43:49 -0400 7, 30 -- Received: from WPVEXCMBX04.purdue.lcl ([169.254.4.15]) by 7, 30 -- WPVEXCHUB03.purdue.lcl ([172.30.136.67]) with mapi id 14.03.0224.002; Tue, 27 7, 30 -- Oct 2015 13:43:49 -0400 7, 30 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 7, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 30 -- Subject: Core facility opening at Purdue 7, 30 -- Thread-Topic: Core facility opening at Purdue 7, 30 -- Thread-Index: AQHREN8JV9AostOkek+DIhUp1r71yQ== 7, 30 -- Date: Tue, 27 Oct 2015 17:43:48 +0000 7, 30 -- Message-ID: {2B9A828E-B45D-46D1-9F89-03BC675A8461-at-purdue.edu} 7, 30 -- Accept-Language: en-US 7, 30 -- Content-Language: en-US 7, 30 -- X-MS-Has-Attach: 7, 30 -- X-MS-TNEF-Correlator: 7, 30 -- x-originating-ip: [10.163.23.192] 7, 30 -- Content-Type: text/plain; charset="utf-8" 7, 30 -- Content-ID: {3B5D3AC0E24DA74CAE18707ED5A97D51-at-exchange.purdue.edu} 7, 30 -- MIME-Version: 1.0 7, 30 -- X-PMX-Version: 6.0.2.2308539 7, 30 -- X-PerlMx-URL-Scanned: Yes 7, 30 -- X-PerlMx-Virus-Scanned: Yes 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t9RHhrj4002867 ==============================End of - Headers==============================
Scanning Electron Microscopy (SEM) Facility Manager Position Available
The NUANCE Center is an active and vibrant user facility housing state-of-the-art instrumentation for materials characterization at Northwestern University. The SEM Facility Manager oversees all aspects of the Scanning Electron Microscopy (SEM) facility in the NUANCE Center, which includes five SEM instruments with a wide range of analytical capabilities. In addition, the SEM Facility Manager has supervisory responsibilities and oversees the related sample preparation facility, including an advanced dual-beam Focused Ion Beam (FIB) instrument.
In addition to microscopy tasks, the instruments in the SEM facility are also used for nanofabrication via electron and ion beam lithography. The SEM Facility Manager is the main interface for facility users on this equipment and is responsible for user training and technical support with the assistance of the Microscopy and Imaging Specialist. In addition, the Facility Manager has responsibilities which include: course development and laboratory teaching for SEM-related curriculum; equipment and consumable purchasing; equipment troubleshooting; development of new analytical techniques and capabilities; providing analytical services for both internal NU and external industrial clients; conducting facility tours and demonstrations. This position requires a unique combination of hands-on technical abilities with strong communication skills for training and teaching and offers excellent professional and academic development opportunities.
FOR MORE INFORMATION AND TO APPLY
http://bit.ly/nuance-sem
Ben Myers SEM/FIB Facility Manager NUANCE Center
Northwestern University Mail: 2036 Cook Hall Office: 1114 Cook Hall 2220 Campus Drive Evanston, IL 60208-3108
ph: (847) 491-3439 fax: (847) 467-6573
http://www.nuance.northwestern.edu
==============================Original Headers============================== 14, 49 -- From b-myers3-at-northwestern.edu Wed Oct 28 17:55:44 2015 14, 49 -- Received: from evcspsym1.ads.northwestern.edu (evcspsym1.ads.northwestern.edu [129.105.238.5]) 14, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9SMtie3011422 14, 49 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2015 17:55:44 -0500 14, 49 -- X-AuditID: 8169ee05-f79526d000005bac-35-5631526cba5d 14, 49 -- Received: from evcspmbx04.ads.northwestern.edu (evcspmbx04.ads.northwestern.edu [165.124.43.176]) 14, 49 -- by evcspsym1.ads.northwestern.edu (Symantec Messaging Gateway) with SMTP id A8.A4.23468.C6251365; Wed, 28 Oct 2015 17:55:40 -0500 (CDT) 14, 49 -- Received: from EVCSPMBX03.ads.northwestern.edu (165.124.43.175) by 14, 49 -- evcspmbx04.ads.northwestern.edu (165.124.43.176) with Microsoft SMTP Server 14, 49 -- (TLS) id 15.0.1076.9; Wed, 28 Oct 2015 17:55:40 -0500 14, 49 -- Received: from EVCSPMBX03.ads.northwestern.edu ([fe80::ac08:7018:da9f:f8df]) 14, 49 -- by evcspmbx03.ads.northwestern.edu ([fe80::ac08:7018:da9f:f8df%12]) with mapi 14, 49 -- id 15.00.1076.000; Wed, 28 Oct 2015 17:55:40 -0500 14, 49 -- From: Benjamin D Myers {b-myers3-at-northwestern.edu} 14, 49 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 49 -- Subject: SEM Facility Manager Position 14, 49 -- Thread-Topic: SEM Facility Manager Position 14, 49 -- Thread-Index: AdER07CbnL/wrav6R2qr/nYlrj0GJw== 14, 49 -- Date: Wed, 28 Oct 2015 22:55:40 +0000 14, 49 -- Message-ID: {fa58811511a94f5c92aea1eccd2d4ccd-at-evcspmbx03.ads.northwestern.edu} 14, 49 -- Accept-Language: en-US 14, 49 -- Content-Language: en-US 14, 49 -- X-MS-Has-Attach: 14, 49 -- X-MS-TNEF-Correlator: 14, 49 -- x-ms-exchange-transport-fromentityheader: Hosted 14, 49 -- x-originating-ip: [129.105.136.8] 14, 49 -- Content-Type: text/plain; charset="iso-8859-1" 14, 49 -- MIME-Version: 1.0 14, 49 -- X-Brightmail-Tracker: H4sIAAAAAAAAA02Tf0wbZRzG87bX7oBeexQK37Vsi5eAbvwsuh+JU6f/SDaiaBSNiZuHPWmz 14, 49 -- UppeYWPTBGfUwUQ2RxfWIaNYlqlMhAgukxCpEigj+yVzQ0yn0M2NgoMQXWcY871eS++/5z7f 14, 49 -- e573ee+bI+Xah6SetNicnMPGWhllItHxbva3udaXjaUFveEtW5Z+ql21DRUtHArJStAbiVtN 14, 49 -- nNVSzTnyn34r0Xyr4T37omZvsLFZUYu+oepRAgn0E+D3310l6jS4FOhS1qNEUkuHEJz7x02I 14, 49 -- DyMIJmeGCeEtLX0ZwdE/DYJW0oVwdfpjJOhU+klYnmyP6BQ6C3yDo1GeDdc/b1eKOg/u/PtL 14, 49 -- hBN0JjR9F8KZJEnRL8CVgxUCRrjEvdFOmaDldDr8FjwpE8vR4O2/KBe1Du5MLytEXQC9HQOE 14, 49 -- qB+Bsb7jCtGbB9ddTUpRZ8MpTyjipehk8B8PEoeRzi05wi2xuCUWt8TShoiv0Fqu+m3eztdU 14, 49 -- GPNYE59nq3Q4zXs4XlhHHmeq6kF4I+9b/lacRY1Hc32IJhGjoko2G0u1CrYa23xoByljdNR4 14, 49 -- CUbqskpTjZnlzbscVVaOZ1KpGwKmVnBZlXU3o6c0xZimrFAbt4e3ck58pg8BKcc2AyHYTGzN 14, 49 -- Ps5RKYb5kIEkmHTqSPa1V7V0OevkdnOcnXPEpq+QJAPU9pewMdnBlXN737FYnbEx9k0U4Qkt 14, 49 -- nUTKrKHKgwWl2jTpQNpHRib4UBGpwqUGI3fh7WwFbymP5qZQtqcwVcVoJHM11SZcUBuD8bxR 14, 49 -- 9Jo+ndIIJWlhaq6yrXTUp1GGZ/FAIxkIcfoMau4erqiT8Hhi7I+ZQbMI7yaFOi2Eq/APFS+J 14, 49 -- v6QAk6Iw0hGoDcJ1kqMsHljoxTn0rQSYOmOE5vl2JXQd85Mw1fJ7EgTOP0iC0192qeBTb50a 14, 49 -- wi1X1fCzb1INt5dPaiAwfiAVFi8MpULwwawOutvOpkH4o2PpcPPD5dUQmO/WQ6vXb4CeulNr 14, 49 -- wHV5ci001vetg67vpxlYbGjNhMCR+ixwtXY+CiPd5x8D/3hPPoy1XMuHidmBx2HRV7sR+s98 14, 49 -- sQm6B89tmsFLkeGlPD+eKyzFyTqlS8kRqCpGo0vJEqA2BuM31tcitfO5woziw55PlvbPg32H 14, 49 -- 99cLf90+1HDCM7L+0uv/XdnJ3t96YH1OsdUwFp5yPCwMLbk6ZMwzLrNjoW+maYCdW2f4bCKp 14, 49 -- xfPDQUNN0ZudG3vK5n405tydurHtfkLd8OaMaflEZljt6fft6vtgrml4YX/z13+YA0N6484X 14, 49 -- e/fdPJE8dJEheDNr3CB38Oz/RhlshhIFAAA= 14, 49 -- Content-Transfer-Encoding: 8bit 14, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9SMtie3011422 ==============================End of - Headers==============================
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Email: oscar.rivera-at-uda.cl Name: Oscar Rivera
Organization: Universidad de Atacama
Title-Subject: [Filtered] Hair sample preparation for SEM/EDS
Message: Dear friends:
I want to ask you about if there is a special sample preparation for a momified hair that I need to analyze by SEM and EDS.
The person who request this analysis wants to see the surface of the hair and assess/discard the presence of heavy metals on the surface.
I have some concers about this, because I don't want to damage the sample (it seems there is only ONE hair!). We have a Zeiss EVO MA 10 with Oxford X-Max 20 EDS detector and variable pressure mode.
We don't have coating or sputtering systems, and I am afraid that I could "burn" the hair when the electron beam hits the sample.
Thanks in advice, I sincerely hope your answer.
Best regards,
ORRL
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==============================Original Headers============================== 17, 40 -- From microscopy.listserver-at-gmail.com Wed Oct 28 18:59:42 2015 17, 40 -- Received: from mail-qk0-f179.google.com (mail-qk0-f179.google.com [209.85.220.179]) 17, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9SNxgcB001174 17, 40 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2015 18:59:42 -0500 17, 40 -- Received: by qkbl190 with SMTP id l190so10683468qkb.2 17, 40 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2015 16:59:38 -0700 (PDT) 17, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 40 -- d=gmail.com; s=20120113; 17, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 17, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 17, 40 -- bh=ZhiH2KeggLEoIHMn8gDv4hIk/p+GciYcxGcwCJ2N28w=; 17, 40 -- b=b9rHkD5ESbV51OClKEWhOIU7zClHh/rzBnBnczAo7C4HVNEkkAoIO52LN0YPNSTNjD 17, 40 -- MjHvos1hYyLuF8zyQn9TFzjXeLlx9gbGFlS+tSTd0IRscXhIY4e9Tnd2xoM/QOKDTW6f 17, 40 -- 4g/iahQxb7c1f+jvVdark5juY94tdjDfF+zJuYQO3UsZ4L+q+5pBNrJ9AEY0Z2UcB6yf 17, 40 -- AMDadC0+Ig9xaike9Ub/q4KAN1OA9RQgcfIq68/UrLNTcDu6UOlvnW5ZOw3UkqF36cln 17, 40 -- F4MM+ikpmKp2kZWpoX/So1zUW3Su5Mdu4XDCLvqzFGpANBk6ljU495OjEbxfC7M9bLl6 17, 40 -- nqxw== 17, 40 -- X-Received: by 10.55.71.81 with SMTP id u78mr29315910qka.81.1446076778223; 17, 40 -- Wed, 28 Oct 2015 16:59:38 -0700 (PDT) 17, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 17, 40 -- by smtp.googlemail.com with ESMTPSA id 22sm5744935qhq.39.2015.10.28.16.59.37 17, 40 -- for {microscopy-at-microscopy.com} 17, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 17, 40 -- Wed, 28 Oct 2015 16:59:37 -0700 (PDT) 17, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 17, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 17, 40 -- {microscopylistserver-noreply-at-microscopy.com} 17, 40 -- Subject: viaWWW:Hair sample preparation for SEM/EDS 17, 40 -- References: {201510261325.t9QDPFkg020754-at-ns.microscopy.com} 17, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 40 -- X-Forwarded-Message-Id: {201510261325.t9QDPFkg020754-at-ns.microscopy.com} 17, 40 -- Message-ID: {56316168.8020802-at-microscopy.com} 17, 40 -- Date: Wed, 28 Oct 2015 18:59:36 -0500 17, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 17, 40 -- Gecko/20100101 Thunderbird/38.3.0 17, 40 -- MIME-Version: 1.0 17, 40 -- In-Reply-To: {201510261325.t9QDPFkg020754-at-ns.microscopy.com} 17, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: whiteto-at-missouri.edu Name: Tommi White
Organization: Central States Microscopy and Microanalysis Society
Title-Subject: [Filtered] CSMMS Meeting: "Microscopy and Microanalysis Applications" in St. Louis, MO
Message: Please join us on October 30th, 2015 in at Washington University in St. Louis, Missouri for an upcoming Central States Microscopy and Microanalysis Meeting entitled "Microscopy & Microanalysis Applications".
We are lucky to have MAS tour speaker Ed Vincenzi who will give a talk on "ÂThe Twin Paradox: A Study of Preservation & Disfigurement of Southworth and Hawes Daguerreotype Photographs".
Also, we will have our annual student presentation competition where we award a $1000 student travel award to attend the national Microscopy and Microanalysis meeting.
Please visit the following website for more details. http://emc.missouri.edu/csmms/
Thank you. Tommi Tommi A. White, Ph.D. CSMMS secretary/webmaster Assistant Professor of Biochemistry Associate Director, Electron Microscopy Core Facility University of Missouri W125 Veterinary Medicine Building 1600 East Rollins Street Columbia, MO 65211 573-882-8304 WhiteTo-at-missouri.edu www.emc.missouri.edu
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==============================Original Headers============================== 18, 41 -- From microscopy.listserver-at-gmail.com Wed Oct 28 19:00:44 2015 18, 41 -- Received: from mail-qk0-f182.google.com (mail-qk0-f182.google.com [209.85.220.182]) 18, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9T00ia3001465 18, 41 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2015 19:00:44 -0500 18, 41 -- Received: by qkcn129 with SMTP id n129so10757624qkc.1 18, 41 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2015 17:00:40 -0700 (PDT) 18, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 18, 41 -- d=gmail.com; s=20120113; 18, 41 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 18, 41 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 18, 41 -- bh=U6csm/BB5ffHkJ4r8zlPXNfM2UoswR8EmHkcWq7xcd0=; 18, 41 -- b=RS9E8PoM3GJJhlGEK9jrF1x2bL/qC+yrIZJ3hzfytINsobgsjF8Fztr33V8Tl+t1RK 18, 41 -- Km7okBpVIGlUC9GTSQYiB5/itjVcJigNMykEO7PUwSmf0zh3G8SgMYNztQV71FvTg6zj 18, 41 -- VK8GdQhw4kwJjfikmJCjvcLNTgC0S7hmIBvgd7clIMACYnUKLzk6fIySaCMFx/WNoLu/ 18, 41 -- JQdbTm6DotNBR05bu050h8q6Sv4DVsiCF2vuGZdr01JGQzR4elYm7gi47jhdu9NS+pL3 18, 41 -- 7EYkjNmyfFtT7sBZpBWeHBF8LfA6plgfWv7cK9/VnnYOWskxfoszZLEnxTr+OMeTnmGe 18, 41 -- r5Cw== 18, 41 -- X-Received: by 10.55.74.19 with SMTP id x19mr34309044qka.28.1446076839976; 18, 41 -- Wed, 28 Oct 2015 17:00:39 -0700 (PDT) 18, 41 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 18, 41 -- by smtp.googlemail.com with ESMTPSA id g97sm18100398qge.6.2015.10.28.17.00.39 18, 41 -- for {microscopy-at-microscopy.com} 18, 41 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 18, 41 -- Wed, 28 Oct 2015 17:00:39 -0700 (PDT) 18, 41 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 18, 41 -- X-Google-Original-From: MicroscopyListserver-NoReply 18, 41 -- {microscopylistserver-noreply-at-microscopy.com} 18, 41 -- Subject: viaWWW:CSMMS Meeting: "Microscopy and Microanalysis Applications" in 18, 41 -- St. Louis, MO 18, 41 -- References: {201510271417.t9REHNpC029351-at-ns.microscopy.com} 18, 41 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 41 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 41 -- X-Forwarded-Message-Id: {201510271417.t9REHNpC029351-at-ns.microscopy.com} 18, 41 -- Message-ID: {563161A7.6020600-at-microscopy.com} 18, 41 -- Date: Wed, 28 Oct 2015 19:00:39 -0500 18, 41 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 18, 41 -- Gecko/20100101 Thunderbird/38.3.0 18, 41 -- MIME-Version: 1.0 18, 41 -- In-Reply-To: {201510271417.t9REHNpC029351-at-ns.microscopy.com} 18, 41 -- Content-Type: text/plain; charset=UTF-8; format=flowed 18, 41 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Message: Free to a good home: a Perkin-Elmer PHI 660 Scanning Auger Microprobe.
Last use around 2010-11, set to be scrapped. Might be working or can be used for parts
It is in Ontario, Canada, you haul it off site.
Interested parties please contact Mandi at MandiH-at-ixrfsystems.com
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==============================Original Headers============================== 16, 40 -- From microscopy.listserver-at-gmail.com Wed Oct 28 19:01:44 2015 16, 40 -- Received: from mail-qg0-f46.google.com (mail-qg0-f46.google.com [209.85.192.46]) 16, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9T01iSR003036 16, 40 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2015 19:01:44 -0500 16, 40 -- Received: by qgem9 with SMTP id m9so21325756qge.1 16, 40 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2015 17:01:40 -0700 (PDT) 16, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 40 -- d=gmail.com; s=20120113; 16, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 16, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 16, 40 -- bh=r5buUxP2dM1KYW3J1qHno6U9JJUHywstfhLBz2dZZGM=; 16, 40 -- b=EJVYUQJKExkoYWRv7oMJK+AMcxSydYyWjM99wwjpDxDjUIM3pqeS+78YNrFzCdtUH9 16, 40 -- /uEfclYSDEu0/49kGRbiP7uSmGp8Mxqit8m66dZBYYa1WY1e4t8Txo/Vgr3z918zs7+5 16, 40 -- XHBhMlAKhMsI965GNGoNttVAUudOEQkRWBtxd7mkBKo9Ek1sZcxcakNGXM4Ufhj+1Oiz 16, 40 -- vRMzWToeh0Q4Q2QAMPn5WRw+riOe/0bjwQksCjx/lI0knh42SE0BlGVlCb4FW+BTdKwz 16, 40 -- DMXYUXMcJkCK5MddulVnnW7HxcGuMe4iH7eQZUSChIUM8wJ0KHtVUZeJt/8oMVVM1wPx 16, 40 -- Agng== 16, 40 -- X-Received: by 10.140.104.104 with SMTP id z95mr2584075qge.75.1446076900022; 16, 40 -- Wed, 28 Oct 2015 17:01:40 -0700 (PDT) 16, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 16, 40 -- by smtp.googlemail.com with ESMTPSA id f81sm15152466qhc.14.2015.10.28.17.01.39 16, 40 -- for {microscopy-at-microscopy.com} 16, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 40 -- Wed, 28 Oct 2015 17:01:39 -0700 (PDT) 16, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 16, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 40 -- {microscopylistserver-noreply-at-microscopy.com} 16, 40 -- Subject: viaWWW:Free Scanning Auger Microprobe 16, 40 -- References: {201510281551.t9SFpdn6000615-at-ns.microscopy.com} 16, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 40 -- X-Forwarded-Message-Id: {201510281551.t9SFpdn6000615-at-ns.microscopy.com} 16, 40 -- Message-ID: {563161E3.8040907-at-microscopy.com} 16, 40 -- Date: Wed, 28 Oct 2015 19:01:39 -0500 16, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 16, 40 -- Gecko/20100101 Thunderbird/38.3.0 16, 40 -- MIME-Version: 1.0 16, 40 -- In-Reply-To: {201510281551.t9SFpdn6000615-at-ns.microscopy.com} 16, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: Free to a good home: a Perkin-Elmer PHI 660 Scanning Auger Microprobe.
Last use around 2010-11, set to be scrapped. Might be working or can be used for parts
It is in Ontario, Canada, you haul it off site.
Interested parties please contact Mandi at MandiH-at-ixrfsystems.com
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==============================Original Headers============================== 16, 40 -- From microscopy.listserver-at-gmail.com Thu Oct 29 07:34:01 2015 16, 40 -- Received: from mail-qg0-f46.google.com (mail-qg0-f46.google.com [209.85.192.46]) 16, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9T01iSR003036 16, 40 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2015 19:01:44 -0500 16, 40 -- Received: by qgem9 with SMTP id m9so21325756qge.1 16, 40 -- for {microscopy-at-microscopy.com} ; Wed, 28 Oct 2015 17:01:40 -0700 (PDT) 16, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 40 -- d=gmail.com; s=20120113; 16, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 16, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 16, 40 -- bh=r5buUxP2dM1KYW3J1qHno6U9JJUHywstfhLBz2dZZGM=; 16, 40 -- b=EJVYUQJKExkoYWRv7oMJK+AMcxSydYyWjM99wwjpDxDjUIM3pqeS+78YNrFzCdtUH9 16, 40 -- /uEfclYSDEu0/49kGRbiP7uSmGp8Mxqit8m66dZBYYa1WY1e4t8Txo/Vgr3z918zs7+5 16, 40 -- XHBhMlAKhMsI965GNGoNttVAUudOEQkRWBtxd7mkBKo9Ek1sZcxcakNGXM4Ufhj+1Oiz 16, 40 -- vRMzWToeh0Q4Q2QAMPn5WRw+riOe/0bjwQksCjx/lI0knh42SE0BlGVlCb4FW+BTdKwz 16, 40 -- DMXYUXMcJkCK5MddulVnnW7HxcGuMe4iH7eQZUSChIUM8wJ0KHtVUZeJt/8oMVVM1wPx 16, 40 -- Agng== 16, 40 -- X-Received: by 10.140.104.104 with SMTP id z95mr2584075qge.75.1446076900022; 16, 40 -- Wed, 28 Oct 2015 17:01:40 -0700 (PDT) 16, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 16, 40 -- by smtp.googlemail.com with ESMTPSA id f81sm15152466qhc.14.2015.10.28.17.01.39 16, 40 -- for {microscopy-at-microscopy.com} 16, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 40 -- Wed, 28 Oct 2015 17:01:39 -0700 (PDT) 16, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 16, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 40 -- {microscopylistserver-noreply-at-microscopy.com} 16, 40 -- Subject: viaWWW:Free Scanning Auger Microprobe 16, 40 -- References: {201510281551.t9SFpdn6000615-at-ns.microscopy.com} 16, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 40 -- X-Forwarded-Message-Id: {201510281551.t9SFpdn6000615-at-ns.microscopy.com} 16, 40 -- Message-ID: {563161E3.8040907-at-microscopy.com} 16, 40 -- Date: Wed, 28 Oct 2015 19:01:39 -0500 16, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 16, 40 -- Gecko/20100101 Thunderbird/38.3.0 16, 40 -- MIME-Version: 1.0 16, 40 -- In-Reply-To: {201510281551.t9SFpdn6000615-at-ns.microscopy.com} 16, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I can't say that I have ever looked at mummified hair in the SEM, but I have examined human hair. In my case, it was before and after images to compare hair care products, so I was forced to examine them uncoated. It can be done if you are careful - I did it with a tungsten filament at around 15 kV and the hair survived.
One of the most important things is to make sure that each end of the hair is grounded electrically. Secondly, start with as low of a kV as possible (I realize you are doing EDS, but depending on the elements you are looking for you may be able to run your scans at lower accelerating voltages). Experimenting with the VP option may also help give you a better image.
Since you only have one hair to work with, you might want to start with a piece of your own hair, and see if you can analyze it without damaging it. That should give you a fair approximation of what to expect, although I don't know what changes may have occurred to the mummified hair over time.
Hopefully this at least gives you a couple of ideas. I'd be happy to try to answer any more questions if they should arise.
Cheers,
Jeff
Jeffrey A. Hall | Microscopist Structure Probe, Inc. | 206 Garfield Ave. | West Chester, PA 19380, USA 1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, October 28, 2015 8:39 PM To: Jeff Hall
X-from: oscar.rivera-at-uda.cl
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Email: oscar.rivera-at-uda.cl Name: Oscar Rivera
Organization: Universidad de Atacama
Title-Subject: [Filtered] Hair sample preparation for SEM/EDS
Message: Dear friends:
I want to ask you about if there is a special sample preparation for a momified hair that I need to analyze by SEM and EDS.
The person who request this analysis wants to see the surface of the hair and assess/discard the presence of heavy metals on the surface.
I have some concers about this, because I don't want to damage the sample (it seems there is only ONE hair!). We have a Zeiss EVO MA 10 with Oxford X-Max 20 EDS detector and variable pressure mode.
We don't have coating or sputtering systems, and I am afraid that I could "burn" the hair when the electron beam hits the sample.
Thanks in advice, I sincerely hope your answer.
Best regards,
ORRL
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==============================Original Headers============================== 34, 29 -- From jhall-at-2spi.com Thu Oct 29 07:34:43 2015 34, 29 -- Received: from mail.2spi.com (mail.2spi.com [209.120.197.107]) 34, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9TCYgSw001966 34, 29 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2015 07:34:43 -0500 34, 29 -- Received: from SPI-EX-01.spi.local (172.25.75.31) by SPI-EX-01.spi.local 34, 29 -- (172.25.75.31) with Microsoft SMTP Server (TLS) id 15.0.847.32; Thu, 29 Oct 34, 29 -- 2015 08:20:04 -0400 34, 29 -- Received: from SPI-EX-01.spi.local ([fe80::f8c4:9b23:d8de:fa70]) by 34, 29 -- SPI-EX-01.spi.local ([fe80::f8c4:9b23:d8de:fa70%12]) with mapi id 34, 29 -- 15.00.0847.040; Thu, 29 Oct 2015 08:20:03 -0400 34, 29 -- From: Jeff Hall {jhall-at-2spi.com} 34, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 34, 29 -- CC: "oscar.rivera-at-uda.cl" {oscar.rivera-at-uda.cl} 34, 29 -- Subject: RE: [Microscopy] viaWWW:Hair sample preparation for SEM/EDS 34, 29 -- Thread-Topic: [Microscopy] viaWWW:Hair sample preparation for SEM/EDS 34, 29 -- Thread-Index: AQHREeIrRAFX30kvZ0i1oOOYzvWE756CX7KA 34, 29 -- Date: Thu, 29 Oct 2015 12:20:03 +0000 34, 29 -- Message-ID: {f5684ad88dbf44058a72081142a6068a-at-SPI-EX-01.spi.local} 34, 29 -- References: {201510290038.t9T0ch8A007215-at-ns.microscopy.com} 34, 29 -- In-Reply-To: {201510290038.t9T0ch8A007215-at-ns.microscopy.com} 34, 29 -- Accept-Language: en-US 34, 29 -- Content-Language: en-US 34, 29 -- X-MS-Has-Attach: 34, 29 -- X-MS-TNEF-Correlator: 34, 29 -- x-originating-ip: [172.25.75.117] 34, 29 -- Content-Type: text/plain; charset="iso-8859-1" 34, 29 -- MIME-Version: 1.0 34, 29 -- Content-Transfer-Encoding: 8bit 34, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9TCYgSw001966 ==============================End of - Headers==============================
I have looked at fresh hair in VP mode without any special preparation. It is definitely a subject to beam damage, do not remember exactly at what magnification, but it should be something in between x1000 and x5000. Just a reminder: when doing EDS in VP mode be sure to place hair on wide enough carbon substrate (could be carbon tape); electron skirt may spread as far as a millimeter or two, and EDS can pick up elements from a specimen stub. Good luck, Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, October 29, 2015 8:54 AM To: Dusevich, Vladimir
X-from: oscar.rivera-at-uda.cl
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both oscar.rivera-at-uda.cl as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: oscar.rivera-at-uda.cl Name: Oscar Rivera
Organization: Universidad de Atacama
Title-Subject: [Filtered] Hair sample preparation for SEM/EDS
Message: Dear friends:
I want to ask you about if there is a special sample preparation for a momified hair that I need to analyze by SEM and EDS.
The person who request this analysis wants to see the surface of the hair and assess/discard the presence of heavy metals on the surface.
I have some concers about this, because I don't want to damage the sample (it seems there is only ONE hair!). We have a Zeiss EVO MA 10 with Oxford X-Max 20 EDS detector and variable pressure mode.
We don't have coating or sputtering systems, and I am afraid that I could "burn" the hair when the electron beam hits the sample.
Thanks in advice, I sincerely hope your answer.
Best regards,
ORRL
Login Host: 146.83.76.136 Listserver Email Form V - 20120416 --------------------------------------------------------------------------- -- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
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============================================
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==============================Original Headers============================== 26, 31 -- From DusevichV-at-umkc.edu Thu Oct 29 15:31:51 2015 26, 31 -- Received: from mst-rip6-umkc-out.um.umsystem.edu (mst-rip6-umkc-out.um.umsystem.edu [198.209.50.152]) 26, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9TKVpmW027266 26, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Oct 2015 15:31:51 -0500 26, 31 -- X-IronPort-Anti-Spam-Filtered: true 26, 31 -- X-IronPort-Anti-Spam-Result: A2BGBQBagTJW/9CeoM9bA4M2U28GwQ8hhXgCgTQ7EQEBAQEBAQF/C4Q1AQEBBDoUJQYMBAIBCBEDAQEBCwIKAQcJByEQARQJCAIECgQFCBUEh3oDEg3ARA1GAYN+AQEBAQEBAQEBAQEBAQEBAQEBAQEBGIZ3hH6CUw0aAYEyEAIBHiERFQEBgwWBFAWHRYVWiSgBhRyFQVGDT0iDd45Ng1+DcjcshARyAYQ1BxcjAYEGAQEB 26, 31 -- X-IPAS-Result: A2BGBQBagTJW/9CeoM9bA4M2U28GwQ8hhXgCgTQ7EQEBAQEBAQF/C4Q1AQEBBDoUJQYMBAIBCBEDAQEBCwIKAQcJByEQARQJCAIECgQFCBUEh3oDEg3ARA1GAYN+AQEBAQEBAQEBAQEBAQEBAQEBAQEBGIZ3hH6CUw0aAYEyEAIBHiERFQEBgwWBFAWHRYVWiSgBhRyFQVGDT0iDd45Ng1+DcjcshARyAYQ1BxcjAYEGAQEB 26, 31 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) 26, 31 -- by mst-rip6-exch-relay.um.umsystem.edu with ESMTP; 29 Oct 2015 15:31:46 -0500 26, 31 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.171]) by 26, 31 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi id 14.03.0248.002; 26, 31 -- Thu, 29 Oct 2015 15:31:45 -0500 26, 31 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 26, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 26, 31 -- CC: "oscar.rivera-at-uda.cl" {oscar.rivera-at-uda.cl} 26, 31 -- Subject: RE: [Microscopy] viaWWW:Hair sample preparation for SEM/EDS 26, 31 -- Thread-Topic: [Microscopy] viaWWW:Hair sample preparation for SEM/EDS 26, 31 -- Thread-Index: AQHRElEyRf86aTpcVUmU+6bHdixMBJ6C61RA 26, 31 -- Date: Thu, 29 Oct 2015 20:31:44 +0000 26, 31 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DCA0A5F-at-UM-MBX-T01.um.umsystem.edu} 26, 31 -- References: {201510291353.t9TDrWX7023472-at-ns.microscopy.com} 26, 31 -- In-Reply-To: {201510291353.t9TDrWX7023472-at-ns.microscopy.com} 26, 31 -- Accept-Language: en-US 26, 31 -- Content-Language: en-US 26, 31 -- X-MS-Has-Attach: 26, 31 -- X-MS-TNEF-Correlator: 26, 31 -- x-originating-ip: [134.193.157.109] 26, 31 -- Content-Type: text/plain; charset="us-ascii" 26, 31 -- MIME-Version: 1.0 26, 31 -- Content-Transfer-Encoding: 8bit 26, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9TKVpmW027266 ==============================End of - Headers==============================
Colleagues, I hope somone can help me with this. When starting a new application, I noticed that the image feed from a CCD camera on a light microscope was fluctuating in intensity. Enough to easily see background flickering when playing back a movie. I want to measure intensity so this is a problem. After troubleshooting, it seems to be casused by fluctuating mains power. I would like to know how I might go about stablizing the power, either with some kind of line conditioner (but what kind?) or perhaps a battery driven solution.
Here is the key experiment that I interpet as implicating the mains. I attached the camera to a macro lens and set up in a dark room (no microscope), a static scene, and compared the following arrangments:
A: light from an IR diode array (driven off mains by an adaptor), 10 msec expsosure B: light from fluorescent room lights, 10 msec expsoure
(The camera mfr rep told me that a 10 msec exposure is fine for this camera, which for the record is an IDS ÂľEye.)
For each, I took a five-frame sequence, with each frame separated by 60 sec. Then measured the average gray level for a box in the middle of the field on each frame. The data are:
A (IR): 159 +/- 3.2 (mean gray +/- SD) B (fluoro): 142 +/- 0.5
The image sequence for B looks totally steady to the eye, while the one for A is easily seen to flicker. I am thinking that some kind of noise in the mains power gets across the DC adaptor driving the LED array. This assumes that the room fluorescents are immune to such noise. Note that the microscope uses a 20 W tungsten/halogen lamp that is powered by an AC stepdown transformer and I think the same noise issue happens there.
From looking at the scene (and live readout of the histogram) the noise seems to be in the 1 to 10 Hz range, but this is simply based on visual inspection.
As I said, some ideas for how to stabilize the microscope's light source would be gratefully (!) appreciated. Thank you.
Tobias
-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ University of Mass. / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ Amherst, Massachusetts / /___ / \ \___/ \_____ USA 413-545-1533 www.bio.umass.edu/biology/baskin
==============================Original Headers============================== 13, 21 -- From baskin-at-bio.umass.edu Thu Oct 29 16:45:04 2015 13, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9TLj49n031794 13, 21 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2015 16:45:04 -0500 13, 21 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 13, 21 -- (authenticated bits=0) 13, 21 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id t9TLixeh021694 13, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO) 13, 21 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2015 17:45:00 -0400 13, 21 -- To: microscnet {microscopy-at-microscopy.com} 13, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 13, 21 -- Subject: fluctuating light problem 13, 21 -- Message-ID: {5632935B.7000106-at-bio.umass.edu} 13, 21 -- Date: Thu, 29 Oct 2015 17:44:59 -0400 13, 21 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.10; rv:38.0) 13, 21 -- Gecko/20100101 Thunderbird/38.3.0 13, 21 -- MIME-Version: 1.0 13, 21 -- Content-Type: text/plain; charset=utf-8; format=flowed 13, 21 -- Content-Transfer-Encoding: 8bit 13, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Thu, 29 Oct 2015 17:45:00 -0400 (EDT) 13, 21 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Knife mark repair using Gatan Digital Micrograph.
Message: Hi, Could any one guide me "how to remove the knife mark from image using gatan digital micrograph." Images are taken in Gatan TEM camera. TIA.
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I would use caution on doing that. It would probably invalidate the use of the photo in a scientific publication since you would be seriously modifying the pixels. One acceptable solution for small defects is to make sure you overlay the defect with an arrow or something as you label the images. As long as you are not intentionally hiding actual data (e.g., an organelle that you say are absent in the cell), I see this as a valid strategy. This seems "fair" since any viewer can deduce that what is under the arrow is not visible whereas a good image processing manipulation to "erase" a knife mark would "fool" many viewers and therefore be unethical.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, October 29, 2015 6:24 PM To: Phillips, Thomas E. {PhillipsT-at-missouri.edu}
X-from: ravi.thakkar369-at-gmail.com
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Knife mark repair using Gatan Digital Micrograph.
Message: Hi, Could any one guide me "how to remove the knife mark from image using gatan digital micrograph." Images are taken in Gatan TEM camera. TIA.
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==============================Original Headers============================== 21, 33 -- From PhillipsT-at-missouri.edu Thu Oct 29 18:30:16 2015 21, 33 -- Received: from um-nip4-missouri-out.um.umsystem.edu (um-nip4-missouri-out.um.umsystem.edu [198.209.49.177]) 21, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9TNUFPO028054 21, 33 -- for {microscopy-at-microscopy.com} ; Thu, 29 Oct 2015 18:30:15 -0500 21, 33 -- X-IronPort-Anti-Spam-Filtered: true 21, 33 -- X-IronPort-Anti-Spam-Result: A2BiBgCNqzJW/8SeoM9bA4M2U28GslOOPCGFeAKBNTsRAQEBAQEBAYEKhDUBAQEEOhQ3AgICAQgRAwEBAQsCCgEHCQcWCxABFAkIAgQBCQkIEwIEh3oDEg3AGw1GAYN+AQEBAQEBAQEBAQEBAQEBAQEBAQEBGASGc4R+glMNEQkBgTIQAgEeBhsRFQEBgwWBFAWHRYVWhBiEYwYnAYUchUFRg09Ig3eOTYdRNyyCD4F1cgEBhDQHFyMBgQYBAQE 21, 33 -- X-IPAS-Result: A2BiBgCNqzJW/8SeoM9bA4M2U28GslOOPCGFeAKBNTsRAQEBAQEBAYEKhDUBAQEEOhQ3AgICAQgRAwEBAQsCCgEHCQcWCxABFAkIAgQBCQkIEwIEh3oDEg3AGw1GAYN+AQEBAQEBAQEBAQEBAQEBAQEBAQEBGASGc4R+glMNEQkBgTIQAgEeBhsRFQEBgwWBFAWHRYVWhBiEYwYnAYUchUFRg09Ig3eOTYdRNyyCD4F1cgEBhDQHFyMBgQYBAQE 21, 33 -- Received: from um-tcas1.um.umsystem.edu ([207.160.158.196]) 21, 33 -- by um-nip4-exch-relay.um.umsystem.edu with ESMTP; 29 Oct 2015 18:30:10 -0500 21, 33 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.105]) by 21, 33 -- UM-TCAS1.um.umsystem.edu ([207.160.158.196]) with mapi id 14.03.0248.002; 21, 33 -- Thu, 29 Oct 2015 18:30:10 -0500 21, 33 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 21, 33 -- To: "ravi.thakkar369-at-gmail.com" {ravi.thakkar369-at-gmail.com} , 21, 33 -- "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 21, 33 -- Subject: RE: [Microscopy] viaWWW:Knife mark repair using Gatan Digital 21, 33 -- Micrograph 21, 33 -- Thread-Topic: [Microscopy] viaWWW:Knife mark repair using Gatan Digital 21, 33 -- Micrograph 21, 33 -- Thread-Index: AQHREqDg6g7VjOegDE+kLBWEl4Sqs56DHKlw 21, 33 -- Date: Thu, 29 Oct 2015 23:30:09 +0000 21, 33 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DE732F60F-at-UM-MBX-T02.um.umsystem.edu} 21, 33 -- References: {201510292323.t9TNNqbV025176-at-ns.microscopy.com} 21, 33 -- In-Reply-To: {201510292323.t9TNNqbV025176-at-ns.microscopy.com} 21, 33 -- Accept-Language: en-US 21, 33 -- Content-Language: en-US 21, 33 -- X-MS-Has-Attach: 21, 33 -- X-MS-TNEF-Correlator: 21, 33 -- x-originating-ip: [128.206.81.115] 21, 33 -- Content-Type: text/plain; charset="us-ascii" 21, 33 -- MIME-Version: 1.0 21, 33 -- Content-Transfer-Encoding: 8bit 21, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9TNUFPO028054 ==============================End of - Headers==============================
We are hereby announcing ICON Europe 2016, the 1st International Conference On Nanoscopy.
We invite you to attend this conference, which will be the first of this newly created series, uniquely focusing on super-resolution light microscopy.
Top speakers of the field are confirmed, including leading international scientists in the field of super-resolution light microscopy, such as Eric Betzig or Markus Sauer.
ICON 2016 will be held at the Biozentrum Basel, Switzerland, on June 7-10, 2016, and will be limited to a maximum of 250 participants. In addition to the 18 invited speakers, additional speakers chosen from submitted Abstracts will give shorter talks. There will also be posters sessions and plenty of time for discussion during free time.
We hope that the conference series will have a successful start with great science, lively discussions, and lots of new ideas arising from the interactions of the attending scientists.
For more information, please visit http://www.icon-europe.org Please feel free to forward this email to anybody who might be interested in this conference.
Best regards, Henning.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
==============================Original Headers============================== 14, 37 -- From henning.stahlberg-at-unibas.ch Fri Oct 30 11:32:08 2015 14, 37 -- Received: from mx1-priv.urz.unibas.ch (mx1-priv.urz.unibas.ch [131.152.226.164]) 14, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9UGW8qs029945 14, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Oct 2015 11:32:08 -0500 14, 37 -- Received: from localhost (localhost [127.0.0.1]) 14, 37 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTP id EAA793E0164 14, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Oct 2015 17:32:03 +0100 (CET) 14, 37 -- X-Virus-Scanned: amavisd-new at unibas.ch 14, 37 -- Received: from mx1-priv.urz.unibas.ch ([131.152.226.164]) 14, 37 -- by localhost (mx1-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 14, 37 -- with LMTP id 9Ste6farnHfx for {Microscopy-at-microscopy.com} ; 14, 37 -- Fri, 30 Oct 2015 17:32:03 +0100 (CET) 14, 37 -- Received: from URZ-HT-CAS-3.urz.unibas.ch (urz-ht-cas-3.urz.unibas.ch [131.152.8.133]) 14, 37 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTPS id D98D43E0144 14, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Oct 2015 17:32:03 +0100 (CET) 14, 37 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 14, 37 -- URZ-HT-CAS-3.urz.unibas.ch ([fe80::cc17:655b:f0b:e110%11]) with mapi id 14, 37 -- 14.03.0248.002; Fri, 30 Oct 2015 17:32:03 +0100 14, 37 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 14, 37 -- To: Microscopy {Microscopy-at-microscopy.com} 14, 37 -- Subject: ICON 2016: 1st International Conference On Nanoscopy in Basel, 14, 37 -- Switzerland, June 7-10, 2016 14, 37 -- Thread-Topic: ICON 2016: 1st International Conference On Nanoscopy in Basel, 14, 37 -- Switzerland, June 7-10, 2016 14, 37 -- Thread-Index: AQHREzCCB4FnnpM0nkm7kD8s56jtHA== 14, 37 -- Date: Fri, 30 Oct 2015 16:32:03 +0000 14, 37 -- Message-ID: {194C3D09-4EA7-4095-89EB-D5E915985C69-at-unibas.ch} 14, 37 -- Accept-Language: en-US, de-CH 14, 37 -- Content-Language: en-US 14, 37 -- X-MS-Has-Attach: 14, 37 -- X-MS-TNEF-Correlator: 14, 37 -- x-originating-ip: [131.152.8.190] 14, 37 -- Content-Type: text/plain; charset="us-ascii" 14, 37 -- Content-ID: {F059F6DDDBFF184BA603FA68E7AA56FD-at-zuv.ads.unibas.ch} 14, 37 -- MIME-Version: 1.0 14, 37 -- Content-Transfer-Encoding: 8bit 14, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9UGW8qs029945 ==============================End of - Headers==============================
} On Oct 29, 2015, at 5:01 PM, PhillipsT-at-missouri.edu wrote: } } I would use caution on doing that. It would probably invalidate the use of the photo in a scientific publication since you would be seriously modifying the pixels. One acceptable solution for small defects is to make sure you overlay the defect with an arrow or something as you label the images. As long as you are not intentionally hiding actual data (e.g., an organelle that you say are absent in the cell), I see this as a valid strategy. This seems "fair" since any viewer can deduce that what is under the arrow is not visible whereas a good image processing manipulation to "erase" a knife mark would "fool" many viewers and therefore be unethical. } } Thomas E. Phillips, Ph.D } } Email: ravi.thakkar369-at-gmail.com } Name: Ravi } } Title-Subject: [Filtered] Knife mark repair using Gatan Digital Micrograph. } } Message: Hi, } Could any one guide me "how to remove the knife mark from image using gatan digital micrograph." } Images are taken in Gatan TEM camera. } TIA. } Dear Ravi & Thomas, The issue of digital manipulations was discussed many years ago, and, at that time, it was agreed that such manipulations were deemed OK if a full explanation of what was done was included in the figure caption and the original image was made available. If the caption contained the words, âA knife mark was removed from this figure using [insert process name here].â I as a reviewer would be OK with it; however, I might ask the author to see the original image if there were any indications that such processing could change the information the author intended to present. One tedious way to remove the knife mark would be to copy nearby pixels and paste them over the mark. Iâm not sure if this can be done in DM, but ImageJ can do it. Yours, Bill
==============================Original Headers============================== 6, 34 -- From wtivol-at-sbcglobal.net Fri Oct 30 18:15:55 2015 6, 34 -- Received: from nm13-vm5.access.bullet.mail.bf1.yahoo.com (nm13-vm5.access.bullet.mail.bf1.yahoo.com [216.109.115.5]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t9UNFt0g016616 6, 34 -- for {microscopy-at-microscopy.com} ; Fri, 30 Oct 2015 18:15:55 -0500 6, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1446246949; bh=5rE72rDNV9Ucjsx3Ox+XQPKPHlOXI/xBGKrYoN35V4I=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=aml8exyvyN6Xpt5HJBNjrlQ0M+gvJJf4594yS3c3J3bVXLYjkA4wBmIpC6X+N0wI4SxFNu83vQd4N79246CYvCXpTy2d8jqmTKJGt4QYuhsW3jyn9eVJSgrwWEKdFh81Nt+gt6v6jIYrvuvoXIgH2i8Fmw8wE/Ha9xdnewf2ESTTOQk/Jbve9GgifUe/GjD44dpCUmN2zAI/bFjzoj8B/Njz9Nn0WA1zYGHo5CY1ss0rtoq4CGPcmCZl/34v+I/XkNvZWNx73qP+DVzPwuvJ6Hx48sblU1KEzfSyGHjBjnNWNt5QkULb+srxm6mV7tCI/QJGB0BMrdZkZj2c9x0Mdw== 6, 34 -- Received: from [66.196.81.160] by nm13.access.bullet.mail.bf1.yahoo.com with NNFMP; 30 Oct 2015 23:15:49 -0000 6, 34 -- Received: from [98.139.244.53] by tm6.access.bullet.mail.bf1.yahoo.com with NNFMP; 30 Oct 2015 23:15:49 -0000 6, 34 -- Received: from [127.0.0.1] by smtp115.sbc.mail.bf1.yahoo.com with NNFMP; 30 Oct 2015 23:15:49 -0000 6, 34 -- X-Yahoo-Newman-Id: 188801.89319.bm-at-smtp115.sbc.mail.bf1.yahoo.com 6, 34 -- X-Yahoo-Newman-Property: ymail-3 6, 34 -- X-YMail-OSG: eXkQzD4VM1kDBeZBKPy81hfSPkuk_lDTVamUESFmnX5wPu. 6, 34 -- pJBQOzueRktdbAoWDibMnwK5ySag_rY2iydHed9hy1J_QUYpobrftiaPwirh 6, 34 -- rwVZYJEH.ImHAx0KRSNNO7LrJF_tGp6IzLyjCzx9xpCDXnZtjlgtvgfGLYfc 6, 34 -- TucxPspbcUJphD4hNGPO9XjU.GRx4zc9Fe8SGwTFjDYygWTvX2WgQNrwr7jF 6, 34 -- uP.iGF.ehZcqXQkR1eXAlaFwEJaPZ2d8U.29iSQMDjh2nP5Owb2EpmtqjZRP 6, 34 -- .ayj2oP30zRWWLIeace56XZ4lumRrHrKeqSse1UhnnX962cs578df.svUX6Q 6, 34 -- aRylmM9_2mXpacIveUqFd2ZdI9XgOaKwqRyXSoCZ8VtbDh04xhDQhDXCaiMi 6, 34 -- zORCOm9qIPaEiKxpma9g9f7hhIrCwXh3CRWxW0sLki9fdz5vWCX9u.yALp5R 6, 34 -- 7NjV3_jXKPPFfzeH2xgdeLHgqbfsTBVqMQaA.mECfk0YduEXduJfYbHHExF. 6, 34 -- E5UGSmgXonIo8jP807fKH_L7GQpM0lpxWWfdpCdmbjPcNj1T2FUYz 6, 34 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 34 -- Content-Type: text/plain; charset=utf-8 6, 34 -- Mime-Version: 1.0 (Mac OS X Mail 9.1 \(3096.5\)) 6, 34 -- Subject: Re: [Microscopy] viaWWW:Knife mark repair using Gatan Digital 6, 34 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 34 -- In-Reply-To: {201510300001.t9U01Jw0024155-at-ns.microscopy.com} 6, 34 -- Date: Fri, 30 Oct 2015 16:15:46 -0700 6, 34 -- Message-Id: {B4611D1D-D3C2-4439-946C-BCB8AD24795A-at-sbcglobal.net} 6, 34 -- References: {201510300001.t9U01Jw0024155-at-ns.microscopy.com} 6, 34 -- To: PhillipsT-at-missouri.edu, ravi.thakkar369-at-gmail.com, 6, 34 -- microscopy-at-microscopy.com 6, 34 -- X-Mailer: Apple Mail (2.3096.5) 6, 34 -- Content-Transfer-Encoding: 8bit 6, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t9UNFt0g016616 ==============================End of - Headers==============================
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From conversion.seo-at-gmail.com Sun Nov 1 14:34:50 2015 Return-Path: {conversion.seo-at-gmail.com} Received: from gmail.com ([118.33.160.52]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tA1KYl5J009332 for {microscopylistserverarchive5-at-microscopy.com} ; Sun, 1 Nov 2015 14:34:49 -0600 Reply-To: conversion.seo-at-gmail.com
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Hi Tobias,
With camera exposure time of 10 mSec you are likely to see flickering of IR of diodes due to ripple of the power supply - line frequency ripple is about 17mSec and after bridge rectification could become 9mSec - both a close to the exposure speed and thus aliasing, Nyquist works.
Driving IR source from a well-filtered DC source or battery would definitely eliminate such ripple. Depending on the design of LED driver, adding electrolytic capacitor of few thousand microfarad to filter rectifier noise could accomplish the same.
Valery Ray - also with AIM Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
On 10/29/2015 5:47 PM, baskin-at-bio.umass.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Colleagues, } I hope somone can help me with this. When starting } a new application, I noticed that the image feed from a CCD camera on a } light microscope was fluctuating in intensity. Enough to easily see } background flickering when playing back a movie. I want to measure } intensity so this is a problem. After troubleshooting, it seems to be } casused by fluctuating mains power. I would like to know how I might go } about stablizing the power, either with some kind of line conditioner } (but what kind?) or perhaps a battery driven solution. } } Here is the key experiment that I interpet as implicating the } mains. I attached the camera to a macro lens and set up in a dark room } (no microscope), a static scene, and compared the following arrangments: } } A: light from an IR diode array (driven off mains by an adaptor), 10 } msec expsosure } B: light from fluorescent room lights, 10 msec expsoure } } (The camera mfr rep told me that a 10 msec exposure is fine for this } camera, which for the record is an IDS ÂľEye.) } } For each, I took a five-frame sequence, with each frame separated by 60 } sec. Then measured the average gray level for a box in the middle of the } field on each frame. The data are: } } A (IR): 159 +/- 3.2 (mean gray +/- SD) } B (fluoro): 142 +/- 0.5 } } The image sequence for B looks totally steady to the eye, while the one } for A is easily seen to flicker. I am thinking that some kind of noise } in the mains power gets across the DC adaptor driving the LED array. } This assumes that the room fluorescents are immune to such noise. Note } that the microscope uses a 20 W tungsten/halogen lamp that is powered by } an AC stepdown transformer and I think the same noise issue happens there. } } From looking at the scene (and live readout of the histogram) the noise } seems to be in the 1 to 10 Hz range, but this is simply based on visual } inspection. } } As I said, some ideas for how to stabilize the microscope's light source } would be gratefully (!) appreciated. Thank you. } } Tobias } }
==============================Original Headers============================== 5, 35 -- From vray-at-partbeamsystech.com Tue Nov 3 07:58:06 2015 5, 35 -- Received: from nm43-vm7.bullet.mail.bf1.yahoo.com (nm43-vm7.bullet.mail.bf1.yahoo.com [216.109.114.238]) 5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tA3Dw3wt002440 5, 35 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2015 07:58:05 -0600 5, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1446559079; bh=Svhg/4IanCJjp3LOeKa0YhqJkD6mGnBTzdIxr2H94DQ=; h=Subject:To:References:Cc:From:Date:In-Reply-To:From:Subject; b=GjBm79kqiecHxzRwiKgBUqumppg3HBOpnoMCrHFhmlNkbB/sfrJeE6dBJodgyAU57VEUEJYh8DmgEos6VdtseP7NKBLumnfLSc6oGbJOOsbxAoE00Wwuhdc4E9ZfXDMIaPXAsqGPjXz0akKMi72F6PhSCziT5sxI8bc//LsZ1HXTeyGTmvgaeq0eca98SxcNYW4QMg7dDX9+bywmhc0MGcGWX8Gafkt7wlGaGNjx1BQBKRs2RqE+6X4MtLrK9pM65sEfN+Shz7YXtLPrJR6JNB8lPwFjLyqQkJAL4zQ6BUPwms2jFoGHberEyadPh9pmkZrP0LGGtBCRkCj3XSwAQA== 5, 35 -- Received: from [66.196.81.173] by nm43.bullet.mail.bf1.yahoo.com with NNFMP; 03 Nov 2015 13:57:59 -0000 5, 35 -- Received: from [98.139.211.160] by tm19.bullet.mail.bf1.yahoo.com with NNFMP; 03 Nov 2015 13:57:59 -0000 5, 35 -- Received: from [127.0.0.1] by smtp217.mail.bf1.yahoo.com with NNFMP; 03 Nov 2015 13:57:59 -0000 5, 35 -- X-Yahoo-Newman-Id: 311891.26720.bm-at-smtp217.mail.bf1.yahoo.com 5, 35 -- X-Yahoo-Newman-Property: ymail-3 5, 35 -- X-YMail-OSG: itnp.4IVM1mfU0Tgy_dHnWTn0xiyI1ux6252hr27bOnK_Va 5, 35 -- roYV.UuVZV6p_EnZxGmujuW8Cumvm9BWGfRBw8SzHbygKFI6nsVR73wxftWH 5, 35 -- L1TN9TmQgYLRzW66ecMeMBIYDFDCO6WhRFTFWc1BPjy_4syLRU2cUD045LJc 5, 35 -- W3xLz_ZtAhk_u_GH2wAZNYk7lUpO.wDDk3CIzN_wv1N_vbHzdtnbYairRROC 5, 35 -- c1gxKcokQlByK0YdegdWArDzpa8iEk5chwHINAsEe2O6ZCEoZVqmx8DbqMdU 5, 35 -- ignjGSpbvUgtD7pzBED76jKHQDQJNgP_O1c1Ch.D4r63ACo0riZVPsD2pS_8 5, 35 -- s_uAXh56iMXNPabvi2p2IEWExIXvU6mvsFPPh9rBdghr7Fj3TkNtx_PqRkcT 5, 35 -- nUx8Jk7J0DQyrUeeiQO7boNLv3OnWJKL60zV3HyfS5GdxxF3k6PXAuaOdH54 5, 35 -- gGCwbHeYI50IrYcZDR_9UJix3vd1wjXQ7p3aulnzOSVpjQE4ZZkyQMlN9nVz 5, 35 -- X0mzuIIHZE4HtZ7DNWLXZhlVBDu.glgiRuHMehoGATgcVk5PGrOyGenMzOKD 5, 35 -- n 5, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 35 -- Subject: Re: [Microscopy] fluctuating light problem 5, 35 -- To: baskin-at-bio.umass.edu 5, 35 -- References: {201510292147.t9TLlq6h000561-at-ns.microscopy.com} 5, 35 -- Cc: microscopy-at-microscopy.com 5, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 5, 35 -- Message-ID: {5638BD66.50309-at-partbeamsystech.com} 5, 35 -- Date: Tue, 3 Nov 2015 08:57:58 -0500 5, 35 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 5, 35 -- Thunderbird/38.3.0 5, 35 -- MIME-Version: 1.0 5, 35 -- In-Reply-To: {201510292147.t9TLlq6h000561-at-ns.microscopy.com} 5, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 5, 35 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I am looking for suggestion on purchasing an UPS system for a 200kV FEG TEM.
We are in need to replace an UPS unit that supports a FEI Tecnai F20, this microscope is used mainly by cryo EM users for single-particle works. The quote weve obtained from Toshiba for a new 1600 XPi costs over $8K, I feel the price is too high and hope to find an alternative that is less pricey.
Thank you very much in advance.
Xinran Nick Liu, M.D. & Ph.D.
Director of CCMI Electron Microscopy Core Facility Research Scientist in Cell Biology Yale University School of Medicine 333 Cedar Street, SHM I-E16A New Haven, CT 06520 Office: 203.785.4050 Lab: 203.785.5390 http://medicine.yale.edu/ccmi/em
==============================Original Headers============================== 11, 33 -- From xinran.liu-at-yale.edu Tue Nov 3 20:07:44 2015 11, 33 -- Received: from vm-emlprdomg-09.its.yale.edu (mail.yale.edu [130.132.50.188]) 11, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tA427iiD031354 11, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2015 20:07:44 -0600 11, 33 -- Received: from x10-caht4.yu.yale.edu (x10-caht4.its.yale.edu [130.132.61.68]) 11, 33 -- by vm-emlprdomg-09.its.yale.edu (8.15.0.59/8.15.0.59) with ESMTPS id tA427dT4022243 11, 33 -- (version=TLSv1 cipher=AES128-SHA bits=128 verify=NOT) 11, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Nov 2015 21:07:39 -0500 11, 33 -- Received: from X10-MBX13.yu.yale.edu ([169.254.13.158]) by 11, 33 -- x10-caht4.yu.yale.edu ([130.132.61.68]) with mapi id 14.03.0235.001; Tue, 3 11, 33 -- Nov 2015 21:07:39 -0500 11, 33 -- From: "Liu, Xinran" {xinran.liu-at-yale.edu} 11, 33 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 33 -- Subject: UPS for TEM 11, 33 -- Thread-Topic: UPS for TEM 11, 33 -- Thread-Index: AdEWpZQ3J5fryAzoSHemkpkDgjkGWg== 11, 33 -- Date: Wed, 4 Nov 2015 02:07:38 +0000 11, 33 -- Message-ID: {F80E859D5560544CB8CDCD16C587B7E17E51F7F8-at-x10-mbx13.yu.yale.edu} 11, 33 -- Accept-Language: en-US 11, 33 -- Content-Language: en-US 11, 33 -- X-MS-Has-Attach: 11, 33 -- X-MS-TNEF-Correlator: 11, 33 -- x-originating-ip: [130.132.61.94] 11, 33 -- Content-Type: text/plain; charset="Windows-1252" 11, 33 -- MIME-Version: 1.0 11, 33 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.15.21,1.0.33,0.0.0000 11, 33 -- definitions=2015-11-04_02:2015-11-03,2015-11-03,1970-01-01 signatures=0 11, 33 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 11, 33 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 11, 33 -- engine=7.0.1-1508030000 definitions=main-1511040039 11, 33 -- X-Yale-Not-Spam: For more info see: http://www.yale.edu/email/spam/content.html 11, 33 -- Content-Transfer-Encoding: 8bit 11, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tA427iiD031354 ==============================End of - Headers==============================
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A client wants us to section some blocks of methacrylate embedded sample for light microscopy. We have a Microm HM 335 E rotary microtome, but we don't have a glass knife carrier. I haven't been able to find any glass knife carriers for this model of rotary microtome. We were hoping to find a relatively inexpensive solution. I was wondering if anyone has any ideas.
Thanks, Shannon
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Tue Nov 3 20:17:47 2015 14, 40 -- Received: from mail-yk0-f176.google.com (mail-yk0-f176.google.com [209.85.160.176]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tA42HlpS007441 14, 40 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2015 20:17:47 -0600 14, 40 -- Received: by ykdr3 with SMTP id r3so49348120ykd.1 14, 40 -- for {microscopy-at-microscopy.com} ; Tue, 03 Nov 2015 18:17:42 -0800 (PST) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=TljscuQkJ/NtFh6cv9oqwVM4BsQvsQ8UwkokwH6dlu4=; 14, 40 -- b=vr/EYRB8mLATjpBmOvKEgasGWmziQrvQcXAxnU0kVTydRApsvztmwwxe6NfatQFMtF 14, 40 -- O96Hdfugcww0Vt/0abR6KFEkw0qfjvufSO13hwySQF1n1+DQYl5AKQJg5Sf7D6aJdql+ 14, 40 -- gdyhmPiF6Jevuq1NLDIaBICRl3hAauToZYU2r8KqnOW41nDmjxb9njoPt45VLK7IyNAZ 14, 40 -- DTYktdORCqbZ8r649KjR9/EaVJw6WCOpt6/KYcHKxNPJRjOEJAatdHN5Ve0tbntd+Ors 14, 40 -- ATdh58nmdSIZo16F/JAIZljziTkC5ZqyOR2PTUaD6DSntShQUotIgMEG/eMeZPtiV4+1 14, 40 -- ieNA== 14, 40 -- X-Received: by 10.31.166.209 with SMTP id p200mr6387360vke.26.1446603462465; 14, 40 -- Tue, 03 Nov 2015 18:17:42 -0800 (PST) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id n84sm8454842vke.21.2015.11.03.18.17.41 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 40 -- Tue, 03 Nov 2015 18:17:41 -0800 (PST) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:Rotary Microtome Glass Knife Carrier 14, 40 -- References: {201511031801.tA3I1LoS010798-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201511031801.tA3I1LoS010798-at-ns.microscopy.com} 14, 40 -- Message-ID: {56396AC5.1050505-at-microscopy.com} 14, 40 -- Date: Tue, 3 Nov 2015 20:17:41 -0600 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.3.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201511031801.tA3I1LoS010798-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
unfortunately no customer or user of MICROM, but (since interested) with Google search for " Microm HM 335 E rotary microtome AND GLASS KNIFE HOLDER " I found the following:
https://www.thermoscientific.com/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/HM%20355S%20Rotary%20Microtome%20387861.pdf Therein: at least once you'll find "glass knives" (setting cutting angle) but I could not find a special hint on glass knife holder - glass knife carrier or else specific.
But the second mentioned document, which can be found at: http://www.bu.edu/becf/downloads/BioInterface%20Technologies/HM%20355S%20Manual.pdf mentions ..... {knife carrier S for glass and diamond knives} .......
As this therefore might be /might have been a special accessory part of the rotary microtome it might also be that you will find answers requesting or asking such at Representatives, or a field office of, or directly, at THERMOFISHER or FISHER Scientific Company -at- https://www.fishersci.com/us/en/contactus.html where you will find:
Contact Us Product & Order Related Questions - US General Customer Service Phone: 1-800-766-7000 Fax: 1-800-926-1166
Mailing Address 300 Industry Drive Pittsburgh, PA 15275 USA
or via https://www.fishersci.com/us/en/catalog/search/products?keyword=MICROM+microtome&nav=
e.g.: Search for: "MICROM glass knife carrier S", in the Search results one finds: Pos. 2 Thermo Scientific Specialized Knife Carriers for Rotary Microtomes with Dovetail Guideway (= https://www.fishersci.com/shop/products/specialized-knife-carriers-rotary-microtomes-dovetail-guideway/454050) Enhance the precision of knife movement with Thermo ScientificT Specialized Knife Carriers for Rotary Microtomes with Dovetail Guideway. Pricing & Availability
as well as: Pos.7 Thermo ScientificT HM 355S Automatic Microtomes Section even the largest specimens with unmatched quality by using the Thermo ScientificT HM 355S Automatic Microtome. Pricing & Availability Specifications
Pos. 9: Thermo ScientificT MicromT Accessories Accessory items for use with the Thermo Scientific Microm product line Pricing & Availability
Just to (honestly) remind you/anyone : Until 30th of September 2014 it was the highly profitable company: MICROM International GmbH Microm International GmbH Robert-Bosch-Str. 49 69190 Walldorf (Baden) Baden-Württemberg GERMANY which produced the MICROM HM 335E rotary microtome...BUT on 30th of September 2014 Thermofisher closed that Company to China and UK/GB.
Unfortunately I do not know whether the also found URL: http://www.medicregister.com/Microm_International_GmbH/Supplier/sid1488.htm is a real and active one..but you could - at least - try to get into contact with {Microm_International_GmbH} Click Here To EMAIL INQUIRY (=http://www.medicregister.com/Microm_International_GmbH/rfq/sid1488.htm) Web: http://www.microm.de E-Mail: infomicrom.de Address: Robert-Bosch-Str. 49, Walldorf D-69190, Germany Phone: +49-(6227)-836-0 | Fax: +49-(6227)-836-111
Best wishes and good luck, Wolfgang
OR Dr. phil. Wolfgang Muss* Head of EM-Lab (retiring 30th November 2015) Honorary Secretary of SCUR (Society for Cutaneous Ultrastructure Research): see Announcements below Member of Works Council SALK-LKH & Central Works Council SALK Safety Counsellor SALK-LKH
(also) Member of the Microscopical Society of America University Institute of Pathology, SALK-LKH (Salzburger Landeskliniken gemeinnuetzige GesmbH, Landeskrankenhaus = Federal State General Hosp.) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE Web(German): http://ww.salk.at Web(German): http://www.pmu.ac.at Phone work: +43+662+4482+4720 Mobile phone work: +43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W. Muss") E-Mail work: W.Muss-at-SALK.at Private Address: Ignaz-Rieder-Kai 19/6, A-5020 SALZBURG, AUSTRIA Mobile-phone private: ++43+676+5 369-456 E-Mail private: wij.muss-at-aon.at
*)registered in: www.researchgate.net (URL: http://www.researchgate.net/profile/Wolfgang_MUSS/ ) [NB.: Registration (free of charges)] ResearchGATE is the leading professional network for scientists. It's free of charge and designed to meet researchers' needs More than 7,000,000 scientists from over 193 countries have already joined! Join 7 million researchers, including 45 Nobel Laureates
------------------------------------------------------------------------- SCUR-Secretary: Information on behalf of Society for Cutaneous Ultrastructure Research (SCUR) - The SKIN IMAGING Society Visit the WEBSITE of SCUR - The Skin Imaging Society at } http://www.scur.org { ------------------------------------------------------------------------- 2016 The 43rd Ann. SCUR meeting will take place during the first 2 days of the 16th European Microscopy Congress - EMC2016 (http://www.emc2016.fr/en/), 29th-30th August 2016 in LYON, France. i.e., Monday afternoon from 2 PM to 7 PM and Tuesday whole day. We expect some 30 oral presentations and 3 guest lectures + posters.
============================================================================================= Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Mittwoch, 04. November 2015 03:28 An: Muß Wolfgang Betreff: [Microscopy] Rotary Microtome Glass Knife Carrier
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Hi Everyone,
A client wants us to section some blocks of methacrylate embedded sample for light microscopy. We have a Microm HM 335 E rotary microtome, but we don't have a glass knife carrier. I haven't been able to find any glass knife carriers for this model of rotary microtome. We were hoping to find a relatively inexpensive solution. I was wondering if anyone has any ideas.
Thanks, Shannon
Login Host: 128.4.175.69 Listserver Email Form V - 20120416 --------------------------------------------------------------------------- -- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to Microscopy-at-Microscopy.com ============================================ ==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Tue Nov 3 20:17:47 2015 14, 40 -- Received: from mail-yk0-f176.google.com (mail-yk0-f176.google.com [209.85.160.176]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tA42HlpS007441 14, 40 -- for {microscopy-at-microscopy.com} ; Tue, 3 Nov 2015 20:17:47 -0600 14, 40 -- Received: by ykdr3 with SMTP id r3so49348120ykd.1 14, 40 -- for {microscopy-at-microscopy.com} ; Tue, 03 Nov 2015 18:17:42 -0800 (PST) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=TljscuQkJ/NtFh6cv9oqwVM4BsQvsQ8UwkokwH6dlu4=; 14, 40 -- b=vr/EYRB8mLATjpBmOvKEgasGWmziQrvQcXAxnU0kVTydRApsvztmwwxe6NfatQFMtF 14, 40 -- O96Hdfugcww0Vt/0abR6KFEkw0qfjvufSO13hwySQF1n1+DQYl5AKQJg5Sf7D6aJdql+ 14, 40 -- gdyhmPiF6Jevuq1NLDIaBICRl3hAauToZYU2r8KqnOW41nDmjxb9njoPt45VLK7IyNAZ 14, 40 -- DTYktdORCqbZ8r649KjR9/EaVJw6WCOpt6/KYcHKxNPJRjOEJAatdHN5Ve0tbntd+Ors 14, 40 -- ATdh58nmdSIZo16F/JAIZljziTkC5ZqyOR2PTUaD6DSntShQUotIgMEG/eMeZPtiV4+1 14, 40 -- ieNA== 14, 40 -- X-Received: by 10.31.166.209 with SMTP id p200mr6387360vke.26.1446603462465; 14, 40 -- Tue, 03 Nov 2015 18:17:42 -0800 (PST) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id n84sm8454842vke.21.2015.11.03.18.17.41 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 40 -- Tue, 03 Nov 2015 18:17:41 -0800 (PST) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:Rotary Microtome Glass Knife Carrier 14, 40 -- References: {201511031801.tA3I1LoS010798-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201511031801.tA3I1LoS010798-at-ns.microscopy.com} 14, 40 -- Message-ID: {56396AC5.1050505-at-microscopy.com} 14, 40 -- Date: Tue, 3 Nov 2015 20:17:41 -0600 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.3.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201511031801.tA3I1LoS010798-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Greetings Fellow Microscopists,
The Midwest Microscopy and Microanalysis Society will hold its 2015 Fall meeting on Friday, November 20th, at Baxter Healthcare Corporate Headquarters in Deerfield, IL. The program will showcase talks presented at M&M 2015 from the Midwest, and a variety of interesting speakers have been invited to share their M&M presentations with you. Further details can be found on the M3S website under Meetings:
http://www.midwestmicroscopy.org/
Please plan to attend this informative program and meet with your local colleagues and vendors. Contact Karl Hagglund (secretary-at-midwestmicroscopy.org) for details and to RSVP.
Elaine Schumacher (on behalf of M3S)
Elaine F. Schumacher | Senior Research Scientist McCrone Associates, Inc. . 850 Pasquinelli Drive . Westmont, IL 60559 P. 630.887.7100 | F. 630.887.7417 | www.mccrone.com ISO/IEC 17025:2005 A2LA Accredited, Certificate #3631.01 . FDA/GDUFA/DEA Registered This email and any files attached may contain information that is legally privileged and/or confidential. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, printing, or copying of this communication is strictly prohibited without our permission.
==============================Original Headers============================== 8, 43 -- From eschumacher-at-mccrone.com Wed Nov 4 15:58:08 2015 8, 43 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 8, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tA4Lw8Ht022596 8, 43 -- for {Microscopy-at-Microscopy.com} ; Wed, 4 Nov 2015 15:58:08 -0600 8, 43 -- X-ASG-Debug-ID: 1446674283-07bbd4246e52290001-FOsErg 8, 43 -- Received: from TMGEX1.tmg.mccrone.com ([192.168.101.73]) by spam.mccrone.com with ESMTP id XjCrRrzsqybLTFFx for {Microscopy-at-Microscopy.com} ; Wed, 04 Nov 2015 15:58:03 -0600 (CST) 8, 43 -- X-Barracuda-Envelope-From: eschumacher-at-mccrone.com 8, 43 -- Received: from TMGEX2.tmg.mccrone.com (192.168.101.74) by 8, 43 -- TMGEX1.tmg.mccrone.com (192.168.101.73) with Microsoft SMTP Server (TLS) id 8, 43 -- 15.0.913.22; Wed, 4 Nov 2015 15:58:02 -0600 8, 43 -- Received: from TMGEX2.tmg.mccrone.com ([::1]) by TMGEX2.tmg.mccrone.com 8, 43 -- ([fe80::ac43:5f4:37be:d22%14]) with mapi id 15.00.0913.011; Wed, 4 Nov 2015 8, 43 -- 15:57:44 -0600 8, 43 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 8, 43 -- To: "'Microscopy-at-Microscopy.com'" {Microscopy-at-Microscopy.com} 8, 43 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society 8, 43 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis 8, 43 -- Society 8, 43 -- X-ASG-Orig-Subj: Meeting Announcement: Midwest Microscopy and Microanalysis Society 8, 43 -- Thread-Index: AdEXSjm64uwP0Fd6T2OUR3bwejkwXw== 8, 43 -- Date: Wed, 4 Nov 2015 21:57:44 +0000 8, 43 -- Message-ID: {c70040c4a7e04e50a8bbf76969196494-at-TMGEX2.tmg.mccrone.com} 8, 43 -- Accept-Language: en-US 8, 43 -- Content-Language: en-US 8, 43 -- X-MS-Has-Attach: 8, 43 -- X-MS-TNEF-Correlator: 8, 43 -- x-vipre-scanned: 0C30C49500AFD00C30C5E2 8, 43 -- x-originating-ip: [192.168.101.76] 8, 43 -- Content-Type: text/plain; charset="iso-8859-1" 8, 43 -- MIME-Version: 1.0 8, 43 -- X-Barracuda-Connect: UNKNOWN[192.168.101.73] 8, 43 -- X-Barracuda-Start-Time: 1446674283 8, 43 -- X-Barracuda-URL: https://spam.mccrone.com:443/cgi-mod/mark.cgi 8, 43 -- X-Virus-Scanned: by bsmtpd at mccrone.com 8, 43 -- X-Barracuda-BRTS-Status: 1 8, 43 -- X-Barracuda-Spam-Score: 0.00 8, 43 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 8, 43 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.24116 8, 43 -- Rule breakdown below 8, 43 -- pts rule name description 8, 43 -- ---- ---------------------- -------------------------------------------------- 8, 43 -- Content-Transfer-Encoding: 8bit 8, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tA4Lw8Ht022596 ==============================End of - Headers==============================
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Email: julio.fernandez-at-cigb.edu.cu Name: Julio
Organization: CIGB
Title-Subject: [Filtered] EVOSŽ FL Auto configuration
Message: Could some one help me with the configuration of the EVOSŽ FL Auto, cat numbers,etc thanks a lot
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Email: DuleyML-at-MiamiOH.edu Name: matt duley
Organization: Miami University
Title-Subject: [Filtered] Bell Bright Spray
Message: Has anyone found a replacement for Bell Bright Spray from SPI? I just found out it is discontinued (OK, I had a pretty large stockpile!) It sure made cleaning the bell jar from our vacuum evaporator easier.
TIA
Matt
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==============================Original Headers============================== 16, 40 -- From microscopy.listserver-at-gmail.com Thu Nov 5 18:04:06 2015 16, 40 -- Received: from mail-yk0-f175.google.com (mail-yk0-f175.google.com [209.85.160.175]) 16, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tA6045p7013429 16, 40 -- for {microscopy-at-microscopy.com} ; Thu, 5 Nov 2015 18:04:05 -0600 16, 40 -- Received: by ykba4 with SMTP id a4so161836434ykb.3 16, 40 -- for {microscopy-at-microscopy.com} ; Thu, 05 Nov 2015 16:04:00 -0800 (PST) 16, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 40 -- d=gmail.com; s=20120113; 16, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 16, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 16, 40 -- bh=8pnbugDFkxPMoKfF6yQOD6m3nx7tGSBKSKJkeQ/gtCc=; 16, 40 -- b=WGfMayx7ObAK5b1gcGQh6JXxAk0EO6GayJaVhwVPfC9cMC4NQT2rCAVOdOu5pjsHZh 16, 40 -- Ws8KQ+xXkjFTf3S/uQ8i+3RuDdr9AkZ2Li0y+CUrMtiPSvgQYsHflJ0rIByooS/XBkLt 16, 40 -- fQNkzkprsso1q/KLScrOeapRcutX+xoqkqgGOXHzIW89B76IwCz+cx2lhiMK4tL01hVh 16, 40 -- iIhlTYxdl7cEOP83zGsVXMxpQaDJPgnEyLmiVqHaYLEl56mRhUTRXy96rAo2QKrVwXsi 16, 40 -- DaQonZujr1vMj3EUQu50EiQh4yHtajwJ5egTdS9cafnVbLC/jIbclkudIC1HAirj4qnw 16, 40 -- ZqgQ== 16, 40 -- X-Received: by 10.31.56.15 with SMTP id f15mr9757998vka.84.1446768240891; 16, 40 -- Thu, 05 Nov 2015 16:04:00 -0800 (PST) 16, 40 -- Received: from mac22.zaluzec.com ([206.69.208.22]) 16, 40 -- by smtp.googlemail.com with ESMTPSA id p78sm6458274vke.26.2015.11.05.16.04.00 16, 40 -- for {microscopy-at-microscopy.com} 16, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 40 -- Thu, 05 Nov 2015 16:04:00 -0800 (PST) 16, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 16, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 40 -- {microscopylistserver-noreply-at-microscopy.com} 16, 40 -- Subject: viaWWW:Bell Bright Spray 16, 40 -- References: {201511051809.tA5I9xmn005539-at-ns.microscopy.com} 16, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 40 -- X-Forwarded-Message-Id: {201511051809.tA5I9xmn005539-at-ns.microscopy.com} 16, 40 -- Message-ID: {563BEE6F.6010100-at-microscopy.com} 16, 40 -- Date: Thu, 5 Nov 2015 18:03:59 -0600 16, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 16, 40 -- Gecko/20100101 Thunderbird/38.3.0 16, 40 -- MIME-Version: 1.0 16, 40 -- In-Reply-To: {201511051809.tA5I9xmn005539-at-ns.microscopy.com} 16, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Evaporate some rock salt after cleaning the bell jar.
John Mardinly, ASU
} On Nov 5, 2015, at 5:34 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: DuleyML-at-MiamiOH.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both DuleyML-at-MiamiOH.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: DuleyML-at-MiamiOH.edu } Name: matt duley } } Organization: Miami University } } Title-Subject: [Filtered] Bell Bright Spray } } Message: Has anyone found a replacement for Bell Bright Spray from SPI? I just found out it is } discontinued (OK, I had a pretty large stockpile!) It sure made cleaning the bell jar from our } vacuum evaporator easier. } } TIA } } Matt } } Login Host: 134.53.244.213 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original Headers============================== } 16, 40 -- From microscopy.listserver-at-gmail.com Thu Nov 5 18:04:06 2015 } 16, 40 -- Received: from mail-yk0-f175.google.com (mail-yk0-f175.google.com [209.85.160.175]) } 16, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tA6045p7013429 } 16, 40 -- for {microscopy-at-microscopy.com} ; Thu, 5 Nov 2015 18:04:05 -0600 } 16, 40 -- Received: by ykba4 with SMTP id a4so161836434ykb.3 } 16, 40 -- for {microscopy-at-microscopy.com} ; Thu, 05 Nov 2015 16:04:00 -0800 (PST) } 16, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 40 -- d=gmail.com; s=20120113; } 16, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent } 16, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; } 16, 40 -- bh=8pnbugDFkxPMoKfF6yQOD6m3nx7tGSBKSKJkeQ/gtCc=; } 16, 40 -- b=WGfMayx7ObAK5b1gcGQh6JXxAk0EO6GayJaVhwVPfC9cMC4NQT2rCAVOdOu5pjsHZh } 16, 40 -- Ws8KQ+xXkjFTf3S/uQ8i+3RuDdr9AkZ2Li0y+CUrMtiPSvgQYsHflJ0rIByooS/XBkLt } 16, 40 -- fQNkzkprsso1q/KLScrOeapRcutX+xoqkqgGOXHzIW89B76IwCz+cx2lhiMK4tL01hVh } 16, 40 -- iIhlTYxdl7cEOP83zGsVXMxpQaDJPgnEyLmiVqHaYLEl56mRhUTRXy96rAo2QKrVwXsi } 16, 40 -- DaQonZujr1vMj3EUQu50EiQh4yHtajwJ5egTdS9cafnVbLC/jIbclkudIC1HAirj4qnw } 16, 40 -- ZqgQ== } 16, 40 -- X-Received: by 10.31.56.15 with SMTP id f15mr9757998vka.84.1446768240891; } 16, 40 -- Thu, 05 Nov 2015 16:04:00 -0800 (PST) } 16, 40 -- Received: from mac22.zaluzec.com ([206.69.208.22]) } 16, 40 -- by smtp.googlemail.com with ESMTPSA id p78sm6458274vke.26.2015.11.05.16.04.00 } 16, 40 -- for {microscopy-at-microscopy.com} } 16, 40 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 16, 40 -- Thu, 05 Nov 2015 16:04:00 -0800 (PST) } 16, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} } 16, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply } 16, 40 -- {microscopylistserver-noreply-at-microscopy.com} } 16, 40 -- Subject: viaWWW:Bell Bright Spray } 16, 40 -- References: {201511051809.tA5I9xmn005539-at-ns.microscopy.com} } 16, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 16, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 16, 40 -- X-Forwarded-Message-Id: {201511051809.tA5I9xmn005539-at-ns.microscopy.com} } 16, 40 -- Message-ID: {563BEE6F.6010100-at-microscopy.com} } 16, 40 -- Date: Thu, 5 Nov 2015 18:03:59 -0600 } 16, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) } 16, 40 -- Gecko/20100101 Thunderbird/38.3.0 } 16, 40 -- MIME-Version: 1.0 } 16, 40 -- In-Reply-To: {201511051809.tA5I9xmn005539-at-ns.microscopy.com} } 16, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 16, 40 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 41 -- From John.Mardinly-at-asu.edu Fri Nov 6 10:34:45 2015 6, 41 -- Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) 6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tA6GYhBY019071 6, 41 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Nov 2015 10:34:43 -0600 6, 41 -- X-ASG-Debug-ID: 1446827666-0549c41decdba330005-FOsErg 6, 41 -- Received: from exhubt02.asurite.ad.asu.edu (exhubt02.asurite.ad.asu.edu [129.219.103.41]) by bcnetw3.asu.edu with ESMTP id E8bFv11dRXYbHvYD (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NO); Fri, 06 Nov 2015 09:34:34 -0700 (MST) 6, 41 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 6, 41 -- X-Barracuda-Apparent-Source-IP: 129.219.103.41 6, 41 -- X-ASG-Whitelist: Client 6, 41 -- Received: from EXMBT01.asurite.ad.asu.edu ([129.219.103.44]) by 6, 41 -- exhubt02.asurite.ad.asu.edu ([129.219.103.41]) with mapi id 14.03.0266.001; 6, 41 -- Fri, 6 Nov 2015 09:34:15 -0700 6, 41 -- From: John Mardinly {John.Mardinly-at-asu.edu} 6, 41 -- To: MSA {Microscopy-at-Microscopy.com} , 6, 41 -- "DuleyML-at-MiamiOH.edu" 6, 41 -- {DuleyML-at-MiamiOH.edu} 6, 41 -- Subject: Re: [Microscopy] viaWWW:Bell Bright Spray 6, 41 -- Thread-Topic: [Microscopy] viaWWW:Bell Bright Spray 6, 41 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW:Bell Bright Spray 6, 41 -- Thread-Index: AQHRGCrtaDJ2EeYyNUSaD4Rppekc8Z6PpvQA 6, 41 -- Date: Fri, 6 Nov 2015 16:34:14 +0000 6, 41 -- Message-ID: {B0FC199E-C280-4EAA-8698-53BFFE6BA497-at-asu.edu} 6, 41 -- References: {201511060034.tA60YgeU006405-at-ns.microscopy.com} 6, 41 -- In-Reply-To: {201511060034.tA60YgeU006405-at-ns.microscopy.com} 6, 41 -- Accept-Language: en-US 6, 41 -- Content-Language: en-US 6, 41 -- X-MS-Has-Attach: 6, 41 -- X-MS-TNEF-Correlator: 6, 41 -- x-originating-ip: [129.219.103.4] 6, 41 -- Content-Type: text/plain; charset="us-ascii" 6, 41 -- Content-ID: {A857753F8CDB6941A667D1F04B252160-at-exchange.asu.edu} 6, 41 -- MIME-Version: 1.0 6, 41 -- X-Barracuda-Connect: exhubt02.asurite.ad.asu.edu[129.219.103.41] 6, 41 -- X-Barracuda-Start-Time: 1446827674 6, 41 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA 6, 41 -- X-Barracuda-URL: https://149.169.2.73:443/cgi-mod/mark.cgi 6, 41 -- Received-SPF: softfail (asu.edu: domain of transitioning john.mardinly-at-asu.edu does not designate 129.219.103.44 as permitted sender) 6, 41 -- X-Virus-Scanned: by bsmtpd at asu.edu 6, 41 -- X-Barracuda-BRTS-Status: 1 6, 41 -- Content-Transfer-Encoding: 8bit 6, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tA6GYhBY019071 ==============================End of - Headers==============================
From ohn.bullocknewsmjpok-at-gmail.com Fri Nov 6 20:31:37 2015 Return-Path: {ohn.bullocknewsmjpok-at-gmail.com} Received: from gmail.com ([58.64.200.23]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id tA72VWYJ017627 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 6 Nov 2015 20:31:34 -0600 Message-ID: {79874E03.A100FCC3-at-gmail.com}
HI, Im decomissioning a lab refrigerator and came across 4 substrate kits from Vector Laboratories. Their documentation only tells me the concentration of solvents in Reagent 3 in each of the kits, which I suspect contains the actual chromogenic substrate. But, the docs do not tell which reagent bottle, nor identifies the substrate. Most of the 3-4 reagent bottles have colored contents.
Unfortunately, our hazardous waste office wont take the solvents unless I can tell them what else is in them. Its been decades since Ive worked with enzymatic labeling and forgotten most details of chromogenic substrates. Does anyone have an idea what is in these kits? Vector just told me to pool the substrates and hand them off. I understand their proprietary interests, but disposal of unknowns is costly. SK-3100 Peroxidase VIP SK-3200 Alkaline Phosphatase Kit II SK-4600 Glucose Oxidase Kit I SK-5200 Glucose Oxidase Kit II
thanks. Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Cellular Morphology Core Center on Human Development and Disability Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-uw.edu
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X-from: henrik.kaker-at-guest.arnes.si To: Zaluzec-at-MICROSCOPY.COM
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Email: henrik.kaker-at-guest.arnes.si Name: Henrik Kaker
Organization: SEM-EDS Lab, Metal Ravne, Slovenia
Title-Subject: [Filtered] Manual for a Polaron Carbon Evaporation Power Supply
Message: Dear All,
Does anyone have a manual for a Polaron Carbon Evaporation Power Supply. Please see the link, http://www.kaker.com/test/polaron.jpg. Thank you.
Regards,
Henrik
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Message: Hello Area Microscopists and Microanalysts
The fall meeting of the Mountain States Society of Electron Microscopists and Colorado Microbeam Analysis Society (MSSEM/CMAS) has been set. Please plan to join us!
Wednesday December 9, 2015 from 6:00 PM to 9:00 PM
CB & Potts (on Collindale Golf Course) -- southwest style buffet and cash bar 1441 East Horsetooth Road Fort Collins, CO 80525 Carpool opportunities available from west Denver area
INVITED SPEAKERS Dr. Andreas Hoenger, University of Colorado "Cryo-EM studies on Microtubule-MAP interactions in vitro and in situ"
Dr. Ai Leen Koh, Stanford University "In Situ and Environmental High Resolution Electron Microscopy of Material Reactions"
POSTERS Posters of current research by students and professionals are invited. Please submit poster abstracts to hlowers-at-usgs.gov by December 6.
RSVP by December 6 to hlowers-at-usgs.gov $30 nonmember $20 MSSEM/CMAS member $10 student
Please send payment to and make check out to MSSEM/CMAS C/O John Chandler 423 Buckeye St. Fort Collins, CO 80524
In addition to the meeting,Gatan and JEOL are hosting a free workshop December 9 and 10 from 9:00 AM to 5:00 PM at Colorado State University. Wednesday's focus will be on in-situ EM with heating and tomography and Thursday's will be on STEM diffraction imaging.
More details will soon be available on the MSSEM/CMAS website http://comas.geoloweb.ch/
Hope to see you there!
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Hi All, I've just sent a PDF copy of the manual to Henrik. If anyone is interested in this PDF, I can share it on our server (~2.9 MB).
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology CAS, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Tue, 10 Nov 2015 02:24:38 -0600, zaluzec-at-microscopy.com wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: henrik.kaker-at-guest.arnes.si } To: Zaluzec-at-MICROSCOPY.COM } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both henrik.kaker-at-guest.arnes.si as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: henrik.kaker-at-guest.arnes.si } Name: Henrik Kaker } } Organization: SEM-EDS Lab, Metal Ravne, Slovenia } } Title-Subject: [Filtered] Manual for a Polaron Carbon Evaporation } Power Supply } } Message: Dear All, } } Does anyone have a manual for a Polaron Carbon Evaporation Power } Supply. Please see the link, http://www.kaker.com/test/polaron.jpg. } Thank you. } } Regards, } } Henrik } } Login Host: 193.189.163.74 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } -- } ============================================= } Do no rely to this messaage it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================== } } ==============================Original } Headers============================== 15, 42 -- From } nestor.zaluzec-at-gmail.com Tue Nov 10 02:17:59 2015 15, 42 -- Received: } from mail-wm0-f45.google.com (mail-wm0-f45.google.com [74.125.82.45]) } 15, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id tAA8HxwT029846; 15, 42 -- Tue, 10 Nov 2015 02:17:59 } -0600 15, 42 -- Received: by wmdw130 with SMTP id } w130so59970934wmd.0; 15, 42 -- Tue, 10 Nov 2015 00:17:54 } -0800 (PST) 15, 42 -- DKIM-Signature: v=1; a=rsa-sha256; } c=relaxed/relaxed; 15, 42 -- d=gmail.com; s=20120113; 15, 42 } -- } h=sender:from:subject:references:to:reply-to:cc:message-id:date 15, } 42 -- :user-agent:mime-version:in-reply-to:content-type 15, } 42 -- :content-transfer-encoding; 15, 42 -- } bh=onhjlbF11rP/x6x6bhf+0YEIJ2QY4yliKs2WbAemBfs=; 15, 42 -- } b=hFOcZ/3+lvjHAI3ULaMFkoyukW0JUCJku6hna3BDpWCU3blanLmnhHRFvweEdbEYLY } 15, 42 -- } yjnqHlMqbx8duADiXEUz1l03gNqbVMd6vo1uZJL+IwV3XfQr6CNyM5KxRN2QRarc2Tpt } 15, 42 -- } bDcojYiRNRxJ7FlLFKdg1+OEEQmcv6y/Os2WkPT/w0FyZ8G9k17bYCIGLAFHSDGyaj80 } 15, 42 -- } 9fgItt/xVZaHvukzDYDYeZc41wDtaMw7wd09gMxJnVVf/Wo+MIzktHRgVyXiER8wUlrz } 15, 42 -- } 5Rcxn8xeeOz9g1Hh0XU6LZhpf5C88JHlmt7MPEpBoQK2SrW9H519MAokkUQvt+wStNMj } 15, 42 -- 3hyg== 15, 42 -- X-Received: by 10.194.179.35 with
On Tue, 10 Nov 2015 10:19:52 +0000, Rick Hughes {rick.hughes-at-microanalysis.com.au} wrote : } Yes please. We operate an old Polaron carbon evaporative coater. It } must be close to 30 years old and still going strong. } } } Rick Hughes, B.Sc.(Hons) Physics, MAIP, MAICD } Managing Director and Principal Consultant } } [Description: microanalysis_logo_12.5%.jpg] } [EmailSignature_Finalist_Blue] } } Suites 5 & 6, 642 Albany Highway, Victoria Park, WA 6100 } M: 040 777 1447 } P: +61 8 9472 4880 } E: } rick.hughes-at-microanalysis.com.au {mailto:rick.hughes-at-microanalysis.com.au} } W: www.microanalysis.com.au {http://www.microanalysis.com.au/} } [facebook4] https://www.facebook.com/microanalysisaustralia } } -----Original Message----- } From: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz] } Sent: Tuesday, 10 November 2015 5:59 PM } To: Rick Hughes } Subject: [Microscopy] Re: viaWWW:Manual for a Polaron Carbon } Evaporation } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi All, } } I've just sent a PDF copy of the manual to Henrik. If anyone is } interested in this PDF, I can share it on our server (~2.9 MB). } } } } Best regards Oldrich } } } } -- } } Oldrich Benada } } Institute of Microbiology CAS, v.v.i. } } Videnska 1083 } } 142 20 Prague 4 } } Czech Republic } } } } On Tue, 10 Nov 2015 02:24:38 -0600, } zaluzec-at-microscopy.com {mailto:zaluzec-at-microscopy.com} wrote : } } } } } } } } } } } } ---------------------------------------------------------------------- } } } ------ The Microscopy ListServer -- CoSponsor: The Microscopy } } Society } } } of America To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver On-Line Help }
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==============================Original Headers============================== 9, 47 -- From benada-at-biomed.cas.cz Tue Nov 10 04:29:20 2015 9, 47 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 9, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAAATKbt028849 9, 47 -- for {microscopy-at-microscopy.com} ; Tue, 10 Nov 2015 04:29:20 -0600 9, 47 -- X-ASG-Debug-ID: 1447151354-05011e14102dcfe0001-4CH8be 9, 47 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id keZCsPvmzUJh9EI9 (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); Tue, 10 Nov 2015 11:29:14 +0100 (CET) 9, 47 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 9, 47 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 9, 47 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 9, 47 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 9, 47 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 9, 47 -- (No client certificate requested) 9, 47 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 375582C8071; 9, 47 -- Tue, 10 Nov 2015 11:29:09 +0100 (CET) 9, 47 -- Date: Tue, 10 Nov 2015 11:26:05 +0100 9, 47 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 9, 47 -- To: Rick Hughes {rick.hughes-at-microanalysis.com.au} , 9, 47 -- {microscopy-at-microscopy.com} 9, 47 -- Subject: Re: [Microscopy] Re: viaWWW:Manual for a Polaron Carbon Evaporation 9, 47 -- Message-ID: {20151110112605.57b2241b-at-u117ob02} 9, 47 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: viaWWW:Manual for a Polaron Carbon Evaporation 9, 47 -- In-Reply-To: {A6D33251D4B6A24D83606737A2ECD2B904B6F657-at-MAASBS-SVR.maa.local} 9, 47 -- References: {201511100958.tAA9wtN6018183-at-ns.microscopy.com} 9, 47 -- {A6D33251D4B6A24D83606737A2ECD2B904B6F657-at-MAASBS-SVR.maa.local} 9, 47 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 9, 47 -- =?UTF-8?B?xIxS?= 9, 47 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 9, 47 -- MIME-Version: 1.0 9, 47 -- Content-Type: text/plain; charset=US-ASCII 9, 47 -- Content-Transfer-Encoding: 7bit 9, 47 -- X-IoP-CAS-MailScanner-ID: 375582C8071.56C58 9, 47 -- X-IoP-CAS-MailScanner: Processed 9, 47 -- X-Spam-Status: No 9, 47 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 9, 47 -- X-Barracuda-Start-Time: 1447151354 9, 47 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 9, 47 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 9, 47 -- X-Barracuda-Scan-Msg-Size: 14213 9, 47 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 9, 47 -- X-Barracuda-BRTS-Status: 1 9, 47 -- X-Barracuda-Spam-Score: 0.50 9, 47 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M 9, 47 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.24269 9, 47 -- Rule breakdown below 9, 47 -- pts rule name description 9, 47 -- ---- ---------------------- -------------------------------------------------- 9, 47 -- 0.50 BSF_RULE7568M Custom Rule 7568M ==============================End of - Headers==============================
Have you designed a facility in a humid city? If yes, can you please help me?
We are currently planning an expanded EM Facility - the sticking point is humidity control.
Our building maintenance / construction team want to know how other EM facilities regulate humidity in rooms with fumehoods. They have the logical comment that it is energy expensive to dehumidify air that is then pumped into a room with fumehoods because the fumehoods just pump the air out. I understand that - however doing sample prep in a space with 80% humidity is causing us a lot of problems. It is virtually impossible to keep our liquid nitrogen ice free and despite our best efforts we have a horrendously high sample mortality rate. We need to reduce the humidity.
And so we are looking for guidance on innovative solutions others have found. At this point any ideas, or comments are most welcome!
Thank-you for your time Erin
_________________________________ DrÂŞ Erin Tranfield Head of the Electron Microscopy Facility Instituto Gulbenkian de CiĂŞncia Rua da Quinta Grande, 6 2780-156 Oeiras, Portugal Tel: +351 21 446 4691 e-mail: etranfield-at-igc.gulbenkian.pt http://uic.igc.gulbenkian.pt/emf.php
==============================Original Headers============================== 8, 35 -- From etranfield-at-igc.gulbenkian.pt Tue Nov 10 09:19:04 2015 8, 35 -- Received: from mx00.igc.gulbenkian.pt (mx00.igc.gulbenkian.pt [193.137.94.10]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAAFJ2MR009044 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 09:19:03 -0600 8, 35 -- Received: from localhost (localhost [127.0.0.1]) 8, 35 -- by mx00.igc.gulbenkian.pt (Postfix) with ESMTP id 6F3942B60A2 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 15:18:57 +0000 (WET) 8, 35 -- X-Virus-Scanned: Debian amavisd-new at igc.gulbenkian.pt 8, 35 -- Received: from mx00.igc.gulbenkian.pt ([127.0.0.1]) 8, 35 -- by localhost (igc.gulbenkian.pt [127.0.0.1]) (amavisd-new, port 10024) 8, 35 -- with LMTP id eYpuito20axF for {Microscopy-at-Microscopy.com} ; 8, 35 -- Tue, 10 Nov 2015 15:18:55 +0000 (WET) 8, 35 -- Received: from mx03 (mx03.igc.gulbenkian.pt [192.168.50.13]) 8, 35 -- by mx00.igc.gulbenkian.pt (Postfix) with ESMTP id 01A412B60A9 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 15:18:54 +0000 (WET) 8, 35 -- Received: from localhost (localhost [127.0.0.1]) 8, 35 -- by mx03 (Postfix) with ESMTP id E113A62827 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 15:18:54 +0000 (WET) 8, 35 -- Received: from mx03 ([127.0.0.1]) 8, 35 -- by localhost (mx03.igc.gulbenkian.pt [127.0.0.1]) (amavisd-new, port 10024) 8, 35 -- with ESMTP id fIRrvVHN01Yh for {Microscopy-at-Microscopy.com} ; 8, 35 -- Tue, 10 Nov 2015 15:18:54 +0000 (WET) 8, 35 -- Received: from etranfieldmbp.igc.gulbenkian.pt (fw1dmz.igc.gulbenkian.pt [192.168.50.3]) 8, 35 -- by mx03 (Postfix) with ESMTPSA id 7702E625C0 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 15:18:54 +0000 (WET) 8, 35 -- From: Erin Tranfield {etranfield-at-igc.gulbenkian.pt} 8, 35 -- Content-Type: text/plain; charset=utf-8 8, 35 -- Subject: Managing Humidity in EM Facilities 8, 35 -- Message-Id: {5C56BB79-C6C7-4D9C-A875-567AF9138413-at-igc.gulbenkian.pt} 8, 35 -- Date: Tue, 10 Nov 2015 15:18:54 +0000 8, 35 -- To: Microscopy-at-Microscopy.com 8, 35 -- Mime-Version: 1.0 (Mac OS X Mail 9.1 \(3096.5\)) 8, 35 -- X-Mailer: Apple Mail (2.3096.5) 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tAAFJ2MR009044 ==============================End of - Headers==============================
I have a reference EELS spectrum that Iâve collected from the literature and I would like to import it into Digital Micrograph so that I can use it in MLLS fitting. The spectrum is in a two-column CSV file (Energy Loss and Counts) and I am not sure about how to get it into the program. What is the best way to do this?
Thanks! ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
Fume hoods should be closed when not in use, i.e. most of the time. So, they do not pump air out.
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: etranfield-at-igc.gulbenkian.pt [mailto:etranfield-at-igc.gulbenkian.pt] Sent: Tuesday, November 10, 2015 9:21 AM To: Dusevich, Vladimir
Dear All
Have you designed a facility in a humid city? If yes, can you please help me?
We are currently planning an expanded EM Facility - the sticking point is humidity control.
Our building maintenance / construction team want to know how other EM facilities regulate humidity in rooms with fumehoods. They have the logical comment that it is energy expensive to dehumidify air that is then pumped into a room with fumehoods because the fumehoods just pump the air out. I understand that - however doing sample prep in a space with 80% humidity is causing us a lot of problems. It is virtually impossible to keep our liquid nitrogen ice free and despite our best efforts we have a horrendously high sample mortality rate. We need to reduce the humidity.
And so we are looking for guidance on innovative solutions others have found. At this point any ideas, or comments are most welcome!
Thank-you for your time Erin
_________________________________ DrÂŞ Erin Tranfield Head of the Electron Microscopy Facility Instituto Gulbenkian de CiĂŞncia Rua da Quinta Grande, 6 2780-156 Oeiras, Portugal Tel: +351 21 446 4691 e-mail: etranfield-at-igc.gulbenkian.pt http://uic.igc.gulbenkian.pt/emf.php
==============================Original Headers============================== 8, 35 -- From etranfield-at-igc.gulbenkian.pt Tue Nov 10 09:19:04 2015 8, 35 -- Received: from mx00.igc.gulbenkian.pt (mx00.igc.gulbenkian.pt [193.137.94.10]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAAFJ2MR009044 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 09:19:03 -0600 8, 35 -- Received: from localhost (localhost [127.0.0.1]) 8, 35 -- by mx00.igc.gulbenkian.pt (Postfix) with ESMTP id 6F3942B60A2 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 15:18:57 +0000 (WET) 8, 35 -- X-Virus-Scanned: Debian amavisd-new at igc.gulbenkian.pt 8, 35 -- Received: from mx00.igc.gulbenkian.pt ([127.0.0.1]) 8, 35 -- by localhost (igc.gulbenkian.pt [127.0.0.1]) (amavisd-new, port 10024) 8, 35 -- with LMTP id eYpuito20axF for {Microscopy-at-Microscopy.com} ; 8, 35 -- Tue, 10 Nov 2015 15:18:55 +0000 (WET) 8, 35 -- Received: from mx03 (mx03.igc.gulbenkian.pt [192.168.50.13]) 8, 35 -- by mx00.igc.gulbenkian.pt (Postfix) with ESMTP id 01A412B60A9 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 15:18:54 +0000 (WET) 8, 35 -- Received: from localhost (localhost [127.0.0.1]) 8, 35 -- by mx03 (Postfix) with ESMTP id E113A62827 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 15:18:54 +0000 (WET) 8, 35 -- Received: from mx03 ([127.0.0.1]) 8, 35 -- by localhost (mx03.igc.gulbenkian.pt [127.0.0.1]) (amavisd-new, port 10024) 8, 35 -- with ESMTP id fIRrvVHN01Yh for {Microscopy-at-Microscopy.com} ; 8, 35 -- Tue, 10 Nov 2015 15:18:54 +0000 (WET) 8, 35 -- Received: from etranfieldmbp.igc.gulbenkian.pt (fw1dmz.igc.gulbenkian.pt [192.168.50.3]) 8, 35 -- by mx03 (Postfix) with ESMTPSA id 7702E625C0 8, 35 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Nov 2015 15:18:54 +0000 (WET) 8, 35 -- From: Erin Tranfield {etranfield-at-igc.gulbenkian.pt} 8, 35 -- Content-Type: text/plain; charset=utf-8 8, 35 -- Subject: Managing Humidity in EM Facilities 8, 35 -- Message-Id: {5C56BB79-C6C7-4D9C-A875-567AF9138413-at-igc.gulbenkian.pt} 8, 35 -- Date: Tue, 10 Nov 2015 15:18:54 +0000 8, 35 -- To: Microscopy-at-Microscopy.com 8, 35 -- Mime-Version: 1.0 (Mac OS X Mail 9.1 \(3096.5\)) 8, 35 -- X-Mailer: Apple Mail (2.3096.5) 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tAAFJ2MR009044 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 31 -- From DusevichV-at-umkc.edu Thu Nov 12 11:37:30 2015 16, 31 -- Received: from um-tip1-umkc-out.um.umsystem.edu (um-tip1-umkc-out.um.umsystem.edu [198.209.49.138]) 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tACHbUiS029278 16, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Nov 2015 11:37:30 -0600 16, 31 -- X-IronPort-Anti-Spam-Filtered: true 16, 31 -- X-IronPort-Anti-Spam-Result: A2BJCQCgzURW/9KeoM9EFwODO1NvBoQjvAkXCoVvAoE8PBABAQEBAQEBgQqENAEBAQRONwQCAQgRAwEBAQsCGwcxARQJCAIEAQkJCBMCBIgNDTu/MQGEGQEBAQEBAQEBAQEBAQEBAQEBAQEBARiGVIR+gmAaAYFAAQEeBhsRFYIZTRyBFQWHRIYQiHQBhRyJZUmDd45Bg3eDcjgrhARyAQGDejoBgQYBAQE 16, 31 -- X-IPAS-Result: A2BJCQCgzURW/9KeoM9EFwODO1NvBoQjvAkXCoVvAoE8PBABAQEBAQEBgQqENAEBAQRONwQCAQgRAwEBAQsCGwcxARQJCAIEAQkJCBMCBIgNDTu/MQGEGQEBAQEBAQEBAQEBAQEBAQEBAQEBARiGVIR+gmAaAYFAAQEeBhsRFYIZTRyBFQWHRIYQiHQBhRyJZUmDd45Bg3eDcjgrhARyAQGDejoBgQYBAQE 16, 31 -- Received: from um-ncas5.um.umsystem.edu ([207.160.158.210]) 16, 31 -- by um-tip1-exch-relay.um.umsystem.edu with ESMTP; 12 Nov 2015 11:37:24 -0600 16, 31 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.205]) by 16, 31 -- UM-NCAS5.um.umsystem.edu ([207.160.158.210]) with mapi id 14.03.0266.001; 16, 31 -- Thu, 12 Nov 2015 11:37:24 -0600 16, 31 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 16, 31 -- To: "etranfield-at-igc.gulbenkian.pt" {etranfield-at-igc.gulbenkian.pt} , 16, 31 -- "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 16, 31 -- Subject: RE: [Microscopy] Managing Humidity in EM Facilities 16, 31 -- Thread-Topic: [Microscopy] Managing Humidity in EM Facilities 16, 31 -- Thread-Index: AQHRG8tUeFY7QjLuy0qtdyhERwCTUJ6YqYNA 16, 31 -- Date: Thu, 12 Nov 2015 17:37:23 +0000 16, 31 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DCAD727-at-UM-MBX-T01.um.umsystem.edu} 16, 31 -- References: {201511101520.tAAFKUWG009694-at-ns.microscopy.com} 16, 31 -- In-Reply-To: {201511101520.tAAFKUWG009694-at-ns.microscopy.com} 16, 31 -- Accept-Language: en-US 16, 31 -- Content-Language: en-US 16, 31 -- X-MS-Has-Attach: 16, 31 -- X-MS-TNEF-Correlator: 16, 31 -- x-originating-ip: [134.193.156.38] 16, 31 -- Content-Type: text/plain; charset="iso-8859-1" 16, 31 -- MIME-Version: 1.0 16, 31 -- Content-Transfer-Encoding: 8bit 16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tACHbUiS029278 ==============================End of - Headers==============================
Recently, I decided to take the end of a red wine cork and put it into the SEM. I found these crystals, which I believe to be tartaric acid crystals (Although I wouldnât mind some confirmation.) There seems to be a residue of some kind on the surface, and I was wondering if anyone on the list could tell me if it looks like sample prep contamination, or if it is something different altogether?
The images I took (Which arenât the best, but they serve the purpose of a quick glance) are here: http://www.jkraft.net/red-wine-1.jpg and http://www.jkraft.net/red-wine-2.jpg
Thanks in advance for the replies!
âJustin A. Kraft
==============================Original Headers============================== 5, 34 -- From kraftpiano-at-gmail.com Thu Nov 12 15:37:58 2015 5, 34 -- Received: from mail-qk0-f181.google.com (mail-qk0-f181.google.com [209.85.220.181]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tACLbw5t021796 5, 34 -- for {microscopy-at-microscopy.com} ; Thu, 12 Nov 2015 15:37:58 -0600 5, 34 -- Received: by qkas77 with SMTP id s77so29030801qka.0 5, 34 -- for {microscopy-at-microscopy.com} ; Thu, 12 Nov 2015 13:37:54 -0800 (PST) 5, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 34 -- d=gmail.com; s=20120113; 5, 34 -- h=from:content-type:content-transfer-encoding:subject:message-id:date 5, 34 -- :to:mime-version; 5, 34 -- bh=mgTC2XIIBO7ZbvPPTla/xQxYlgTn0WRrfbUY3qZp2PA=; 5, 34 -- b=twVcBj5CH2qr1tVTPlJiLQiCth+yIzx/WzaOg1tq3tieRzOpIsYVS+VhK6pG7cbvkS 5, 34 -- kTQuRO4mQMRXg+F3MSzeHGGB4cpotzi5O1Lcu8tP5EMRkMGlih0bu5dPLgt0g1da4JXG 5, 34 -- AZiXwPX+qexvnU0nu34qzg9XRT8wvKrdVxg4Lhf6Hpw1l0SEa8qDYt420XuM/hs9/v8D 5, 34 -- tGQrXxbjuyTwZhHo9kIAFm68MHXC6VFAaGuIGlN4YlwXU5KfgZIgXR5dTLnbYVcc8Te4 5, 34 -- 2Fc0JzG4SAc75TZs01p8wa7Ly4jajE+QQbtYLxduJCY6q+0WuOr6IegeCNHEYLpyHaa1 5, 34 -- q7+w== 5, 34 -- X-Received: by 10.55.77.4 with SMTP id a4mr17830657qkb.54.1447364273906; 5, 34 -- Thu, 12 Nov 2015 13:37:53 -0800 (PST) 5, 34 -- Received: from justins-mini.jkraft.net (rrcs-208-125-225-220.nys.biz.rr.com. [208.125.225.220]) 5, 34 -- by smtp.gmail.com with ESMTPSA id k9sm4062791qgd.21.2015.11.12.13.37.53 5, 34 -- for {microscopy-at-microscopy.com} 5, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 5, 34 -- Thu, 12 Nov 2015 13:37:53 -0800 (PST) 5, 34 -- From: Justin Kraft {kraftpiano-at-gmail.com} 5, 34 -- Content-Type: text/plain; charset=utf-8 5, 34 -- Subject: Crystals from a wine cork 5, 34 -- Message-Id: {0F603AA8-6AFD-4B0B-A419-3B3706ABE8E1-at-gmail.com} 5, 34 -- Date: Thu, 12 Nov 2015 16:37:53 -0500 5, 34 -- To: microscopy-at-microscopy.com 5, 34 -- Mime-Version: 1.0 (Mac OS X Mail 9.1 \(3096.5\)) 5, 34 -- X-Mailer: Apple Mail (2.3096.5) 5, 34 -- Content-Transfer-Encoding: 8bit 5, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tACLbw5t021796 ==============================End of - Headers==============================
Per the presentation I sent you off-line. It could be:
1A Potassium hydrogen tartrate is a major instability in wines ⢠aka Potassium acid tartrate ⢠aka Potassium bitartrate ⢠aka KHT or C4H4O6HK or 1B Potassium Tartrate Hemihydrate
Or
2A Calcium dâtartrate tetrahydrate Or 2B Calcium dâtartrate hexahydrate
Check the elemental composition by EDX and the Refractive indices and crystal characteristics by polarized light microscopy using the data I sent.
Tony
âŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚâŚ Andrew Anthony âTonyâ Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Thursday, November 12, 2015 4:53 PM To: ph2-at-sprynet.com
Hello all,
Recently, I decided to take the end of a red wine cork and put it into the SEM. I found these crystals, which I believe to be tartaric acid crystals (Although I wouldnââŹâ˘t mind some confirmation.) There seems to be a residue of some kind on the surface, and I was wondering if anyone on the list could tell me if it looks like sample prep contamination, or if it is something different altogether?
The images I took (Which arenââŹâ˘t the best, but they serve the purpose of a quick glance) are here: http://www.jkraft.net/red-wine-1.jpg and http://www.jkraft.net/red-wine-2.jpg
Thanks in advance for the replies!
ââŹâJustin A. Kraft
==============================Original Headers============================== 5, 34 -- From kraftpiano-at-gmail.com Thu Nov 12 15:37:58 2015 5, 34 -- Received: from mail-qk0-f181.google.com (mail-qk0-f181.google.com [209.85.220.181]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tACLbw5t021796 5, 34 -- for {microscopy-at-microscopy.com} ; Thu, 12 Nov 2015 15:37:58 -0600 5, 34 -- Received: by qkas77 with SMTP id s77so29030801qka.0 5, 34 -- for {microscopy-at-microscopy.com} ; Thu, 12 Nov 2015 13:37:54 -0800 (PST) 5, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 34 -- d=gmail.com; s=20120113; 5, 34 -- h=from:content-type:content-transfer-encoding:subject:message-id:date 5, 34 -- :to:mime-version; 5, 34 -- bh=mgTC2XIIBO7ZbvPPTla/xQxYlgTn0WRrfbUY3qZp2PA=; 5, 34 -- b=twVcBj5CH2qr1tVTPlJiLQiCth+yIzx/WzaOg1tq3tieRzOpIsYVS+VhK6pG7cbvkS 5, 34 -- kTQuRO4mQMRXg+F3MSzeHGGB4cpotzi5O1Lcu8tP5EMRkMGlih0bu5dPLgt0g1da4JXG 5, 34 -- AZiXwPX+qexvnU0nu34qzg9XRT8wvKrdVxg4Lhf6Hpw1l0SEa8qDYt420XuM/hs9/v8D 5, 34 -- tGQrXxbjuyTwZhHo9kIAFm68MHXC6VFAaGuIGlN4YlwXU5KfgZIgXR5dTLnbYVcc8Te4 5, 34 -- 2Fc0JzG4SAc75TZs01p8wa7Ly4jajE+QQbtYLxduJCY6q+0WuOr6IegeCNHEYLpyHaa1 5, 34 -- q7+w== 5, 34 -- X-Received: by 10.55.77.4 with SMTP id a4mr17830657qkb.54.1447364273906; 5, 34 -- Thu, 12 Nov 2015 13:37:53 -0800 (PST) 5, 34 -- Received: from justins-mini.jkraft.net (rrcs-208-125-225-220.nys.biz.rr.com. [208.125.225.220]) 5, 34 -- by smtp.gmail.com with ESMTPSA id k9sm4062791qgd.21.2015.11.12.13.37.53 5, 34 -- for {microscopy-at-microscopy.com} 5, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 5, 34 -- Thu, 12 Nov 2015 13:37:53 -0800 (PST) 5, 34 -- From: Justin Kraft {kraftpiano-at-gmail.com} 5, 34 -- Content-Type: text/plain; charset=utf-8 5, 34 -- Subject: Crystals from a wine cork 5, 34 -- Message-Id: {0F603AA8-6AFD-4B0B-A419-3B3706ABE8E1-at-gmail.com} 5, 34 -- Date: Thu, 12 Nov 2015 16:37:53 -0500 5, 34 -- To: microscopy-at-microscopy.com 5, 34 -- Mime-Version: 1.0 (Mac OS X Mail 9.1 \(3096.5\)) 5, 34 -- X-Mailer: Apple Mail (2.3096.5) 5, 34 -- Content-Transfer-Encoding: 8bit 5, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tACLbw5t021796 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 30 -- From ph2-at-sprynet.com Thu Nov 12 20:54:05 2015 24, 30 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69]) 24, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAD2s568015570 24, 30 -- for {microscopy-at-microscopy.com} ; Thu, 12 Nov 2015 20:54:05 -0600 24, 30 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 24, 30 -- s=dk20050327; d=sprynet.com; 24, 30 -- b=BuaZiDct3ZBx6MDSYsigCiCWAXJFaDx9wMUeMHb5at0RUYSPCeiGGso+rz3IhpUo; 24, 30 -- h=Received:From:To:References:In-Reply-To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language:X-ELNK-Trace:X-Originating-IP; 24, 30 -- Received: from [68.58.112.163] (helo=AAHHPdv7) 24, 30 -- by elasmtp-mealy.atl.sa.earthlink.net with esmtpa (Exim 4.67) 24, 30 -- (envelope-from {ph2-at-sprynet.com} ) 24, 30 -- id 1Zx4Uo-0001fN-BI 24, 30 -- for microscopy-at-microscopy.com; Thu, 12 Nov 2015 21:53:50 -0500 24, 30 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 24, 30 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 24, 30 -- References: {201511122153.tACLr3vj027399-at-ns.microscopy.com} 24, 30 -- In-Reply-To: {201511122153.tACLr3vj027399-at-ns.microscopy.com} 24, 30 -- Subject: RE: [Microscopy] Crystals from a wine cork 24, 30 -- Date: Thu, 12 Nov 2015 21:53:46 -0500 24, 30 -- Message-ID: {00bb01d11dbe$843fcae0$8cbf60a0$-at-sprynet.com} 24, 30 -- MIME-Version: 1.0 24, 30 -- Content-Type: text/plain; 24, 30 -- charset="utf-8" 24, 30 -- X-Mailer: Microsoft Outlook 14.0 24, 30 -- Thread-Index: AQGYscZkIZUJNuRUklFoyt3Ocg7tFZ8KGC0g 24, 30 -- Content-Language: en-us 24, 30 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9580f47c3a5672a751e949ee3554dea35350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 24, 30 -- X-Originating-IP: 68.58.112.163 24, 30 -- Content-Transfer-Encoding: 8bit 24, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tAD2s568015570 ==============================End of - Headers==============================
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] Canada Balsam on old glass slide
Message: Greetings,
Here's a "blast from the past" question. I have a set of 100+ year old petrographic [glass] thin section slide [sections are from the Vermont talc district...fascinating]. Sample and coverslip mounted with Canada Balsam. We need to remove the coverslip for SEM and EPMA without also destroying the sample. Tried gentle heating of the slide which softened the slip AND dislodged the sample. No joy. Wondering if anyone had experience or helpful suggestions??
Thanks!
Tom
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==============================Original Headers============================== 16, 43 -- From nestor.zaluzec-at-gmail.com Fri Nov 13 03:05:31 2015 16, 43 -- Received: from mail-wm0-f53.google.com (mail-wm0-f53.google.com [74.125.82.53]) 16, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAD95Sqw009809; 16, 43 -- Fri, 13 Nov 2015 03:05:29 -0600 16, 43 -- Received: by wmww144 with SMTP id w144so19915807wmw.1; 16, 43 -- Fri, 13 Nov 2015 01:05:23 -0800 (PST) 16, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 43 -- d=gmail.com; s=20120113; 16, 43 -- h=sender:from:subject:references:cc:to:reply-to:message-id:date 16, 43 -- :user-agent:mime-version:in-reply-to:content-type 16, 43 -- :content-transfer-encoding; 16, 43 -- bh=BO8XLXnnFGQHYPJg7GuuJSz8M8aS5c5ZUYQz2OWcwfs=; 16, 43 -- b=Xi0Uiua2Ml5rSdaV1eef4nT6mJEvw7yJVo0eSEerwEo6zV0TU1tAtdsN4Cxzy8tTLQ 16, 43 -- cha9XBG9spIpJA5EnzwyrU2bnJzZj1BuR5TK/2I5vjrXJCScbCMRom9onptGqEf7qyTQ 16, 43 -- XIBR/9PKVAFu7fvplGHvwgm1DvqkXFk81OkL7/us6OY6RBZd6/cpADqSMPLH4pXkkDkP 16, 43 -- c+XQxmw9u7N9eT6LDzxvAzHxPZwrMEV4cCpMngRsk86Vk8oBcC16YzgT/7FhrT8axpOI 16, 43 -- yagnOFO+rVD+7d8rE0hnc8NZ1Jj96yRpD0DQlziQ3AXnHLT4v2mgNhQxWitlNwIExstV 16, 43 -- hT0w== 16, 43 -- X-Received: by 10.194.11.67 with SMTP id o3mr21406187wjb.3.1447405523585; 16, 43 -- Fri, 13 Nov 2015 01:05:23 -0800 (PST) 16, 43 -- Received: from nestorsrpro2014.home (host31-50-46-29.range31-50.btcentralplus.com. [31.50.46.29]) 16, 43 -- by smtp.googlemail.com with ESMTPSA id w141sm2886495wmw.24.2015.11.13.01.05.22 16, 43 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 43 -- Fri, 13 Nov 2015 01:05:22 -0800 (PST) 16, 43 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 16, 43 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 16, 43 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 43 -- {microscopylistserver-noreply-at-microscopy.com} 16, 43 -- Subject: viaWWW:Canada Balsam on old glass slide 16, 43 -- References: {201511130355.tAD3tqVQ005104-at-ns.microscopy.com} 16, 43 -- Cc: zaluzec-at-microscopy.com 16, 43 -- To: "zaluzec _Microscopy.Com" {zaluzec-at-microscopy.com} , 16, 43 -- MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 43 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 43 -- X-Forwarded-Message-Id: {201511130355.tAD3tqVQ005104-at-ns.microscopy.com} 16, 43 -- Message-ID: {5645A7D1.2070807-at-microscopy.com} 16, 43 -- Date: Fri, 13 Nov 2015 09:05:21 +0000 16, 43 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 16, 43 -- Gecko/20100101 Thunderbird/38.3.0 16, 43 -- MIME-Version: 1.0 16, 43 -- In-Reply-To: {201511130355.tAD3tqVQ005104-at-ns.microscopy.com} 16, 43 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 43 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
X-from: Maria Castello {maritacastello-at-gmail.com}
Hello Just immerse the slides in Xilol. Italia will taken some time until the bĂĄlsamo dissolves. Good luck! Marita
El 13/11/2015 07:20, {zaluzec-at-microscopy.com {mailto:zaluzec-at-microscopy.com} } escribiĂł:
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Email: tomw-at-uidaho.edu {mailto:tomw-at-uidaho.edu} Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] Canada Balsam on old glass slide
Message: Greetings,
Here's a "blast from the past" question. I have a set of 100+ year old petrographic [glass] thin section slide [sections are from the Vermont talc district...fascinating]. Sample and coverslip mounted with Canada Balsam. We need to remove the coverslip for SEM and EPMA without also destroying the sample. Tried gentle heating of the slide which softened the slip AND dislodged the sample. No joy. Wondering if anyone had experience or helpful suggestions??
Thanks!
Tom
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Filtered] Duct socks
Message: Hi All, I am looking for an old-fashioned type fabric duct sock that can be installed on a suspended ceiling tile. I have looked online but seem to only find the cylinder type.
These are the type that microscopists used to use to control dust and air flow issues inside microtomy stations. They used to have a square metal frame with fabric that hung down like a sock. Does anyone know where one can buy these anymore?
Thank you,
Lita Duraine
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==============================Original Headers============================== 13, 42 -- From nestor.zaluzec-at-gmail.com Sat Nov 14 02:34:38 2015 13, 42 -- Received: from mail-wm0-f49.google.com (mail-wm0-f49.google.com [74.125.82.49]) 13, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAE8YbUZ007591 13, 42 -- for {microscopy-at-microscopy.com} ; Sat, 14 Nov 2015 02:34:37 -0600 13, 42 -- Received: by wmec201 with SMTP id c201so59285772wme.1 13, 42 -- for {microscopy-at-microscopy.com} ; Sat, 14 Nov 2015 00:34:32 -0800 (PST) 13, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 42 -- d=gmail.com; s=20120113; 13, 42 -- h=sender:from:subject:references:to:reply-to:message-id:date 13, 42 -- :user-agent:mime-version:in-reply-to:content-type 13, 42 -- :content-transfer-encoding; 13, 42 -- bh=3ONDCx+BeAXfFWA5smplNhyZeTb0k7kBpjJ/MSNUFLw=; 13, 42 -- b=YGhvi8D9bWepuk+hElcmyIRGo53lfrAibiuYKYsb6+FU89phWjiS+IJtWfn/0ZYR2g 13, 42 -- PpPqxLdJJ2RatVT7Gwhhy6xkPUfv2w6b6+OeHIjUDUcKu99FeWEu96GsZvNZ0wzU/Dqq 13, 42 -- Kma16oTvPjw2Et4WTwgtKbnhBvTBh5Dcnbf92H0/kkgmSz1+e0sPXUO9lRaLERiW9kZP 13, 42 -- e1LwvKC3eiZgh352bVKRbCspK6E030Neje17P6OYcDHhP7AAoN9bMnmIdI5YcKGzxYQC 13, 42 -- DAwCmb5UC4KGMsK8gkulMwNK9y5JKFst65MzoCdqa7/tkr1cWT4eS3OzhKajO8yx8vrD 13, 42 -- wA8Q== 13, 42 -- X-Received: by 10.28.9.204 with SMTP id 195mr8801381wmj.88.1447490072257; 13, 42 -- Sat, 14 Nov 2015 00:34:32 -0800 (PST) 13, 42 -- Received: from nestorsrpro2014.home (host31-50-46-29.range31-50.btcentralplus.com. [31.50.46.29]) 13, 42 -- by smtp.googlemail.com with ESMTPSA id m191sm400214wma.3.2015.11.14.00.34.31 13, 42 -- for {microscopy-at-microscopy.com} 13, 42 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 42 -- Sat, 14 Nov 2015 00:34:31 -0800 (PST) 13, 42 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 13, 42 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 13, 42 -- X-Google-Original-From: MicroscopyListserver-NoReply 13, 42 -- {microscopylistserver-noreply-at-microscopy.com} 13, 42 -- Subject: viaWWW:Duct socks 13, 42 -- References: {201511131752.tADHqmIj031558-at-ns.microscopy.com} 13, 42 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 42 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 42 -- X-Forwarded-Message-Id: {201511131752.tADHqmIj031558-at-ns.microscopy.com} 13, 42 -- Message-ID: {5646F215.7000705-at-microscopy.com} 13, 42 -- Date: Sat, 14 Nov 2015 08:34:29 +0000 13, 42 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 13, 42 -- Gecko/20100101 Thunderbird/38.3.0 13, 42 -- MIME-Version: 1.0 13, 42 -- In-Reply-To: {201511131752.tADHqmIj031558-at-ns.microscopy.com} 13, 42 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From news3409384-at-gmail.com Sat Nov 14 05:52:37 2015 Return-Path: {news3409384-at-gmail.com} Received: from gmail.com ([121.170.179.253]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAEBqW4W004576 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 14 Nov 2015 05:52:36 -0600 Reply-To: news3409384-at-gmail.com
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Hi Erin,
My experience is primarily with cleanrooms. They require more control than you likely need - but this may help.
Generally, dehumidifiers in conjunction with humidifiers are used to adjust relative humidity in controlled environments. Despite inefficiencies, relative humidity in cleanrooms I "built" with several fume hoods can be controlled to say 40.0 +/- 0.5 % - that's a commonly used range for cleanrooms. Not too low for breathing and electrostatic discharge and not too high for condensation related issues like you are referring to (ice in liq. N2). Changing relative humidity during the 24 hour day and seasonal variation can really cause technical issues (variation in sample oxidation, condensation, evaporation rates, optics, etc.) without proper control.
Depending on the size of the controlled area there are a variety of appropriate solutions.
Examples, not endorsements, of equipment manufacturers are Munters for dehumidifiers, Condair for humidifiers. If control is what you need, equipment like this needs to be used together and integrated with very good room temperature control schemes - or it could rain inside the lab!
Hope this gives you some ideas.
John
==============================Original Headers============================== 7, 19 -- From john_elzey-at-hotmail.com Sat Nov 14 15:31:49 2015 7, 19 -- Received: from BLU004-OMC3S17.hotmail.com (blu004-omc3s17.hotmail.com [65.55.116.92]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAELVmui032107 7, 19 -- for {microscopy-at-microscopy.com} ; Sat, 14 Nov 2015 15:31:48 -0600 7, 19 -- Received: from BLU180-W53 ([65.55.116.73]) by BLU004-OMC3S17.hotmail.com over TLS secured channel with Microsoft SMTPSVC(7.5.7601.23008); 7, 19 -- Sat, 14 Nov 2015 13:31:43 -0800 7, 19 -- X-TMN: [G3Z4nXVNY7Ueu3LN+dpKBHPbfdrtvGL6] 7, 19 -- X-Originating-Email: [john_elzey-at-hotmail.com] 7, 19 -- Message-ID: {BLU180-W53C5564F77ECB949B25EEAFC100-at-phx.gbl} 7, 19 -- From: john {john_elzey-at-hotmail.com} 7, 19 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 19 -- Subject: Re: Managing Humidity in EM Facilities 7, 19 -- Date: Sat, 14 Nov 2015 13:31:43 -0800 7, 19 -- Importance: Normal 7, 19 -- Content-Type: text/plain; charset="iso-8859-1" 7, 19 -- MIME-Version: 1.0 7, 19 -- X-OriginalArrivalTime: 14 Nov 2015 21:31:43.0964 (UTC) FILETIME=[DBA2A1C0:01D11F23] 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tAELVmui032107 ==============================End of - Headers==============================
We use the product from DuctSox (www.ductsox.com). I believe that all their products are custom made, so you should be able to get what you need. Talk to one of their salesmen or reps. I would encourage you to make sure the air going into the DuctSox is well filtered unless you want to wash the sox weekly!
No financial interest, just a customer.
Cheers, Henk
On 11/14/2015 3:38 AM, zaluzec-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: duraine-at-bcm.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both duraine-at-bcm.edu as well as the Microscopy } Listserver } --------------------------------------------------------------------------- } } Email: duraine-at-bcm.edu } Name: Lita Duraine } } Organization: Howard Hughes Medical Institute } } Title-Subject: [Filtered] Duct socks } } Message: Hi All, } I am looking for an old-fashioned type fabric duct sock that can be } installed on a suspended ceiling tile. I have looked online but seem to } only find the cylinder type. } } These are the type that microscopists used to use to control dust and } air flow issues inside microtomy stations. They used to have a square } metal frame with fabric that hung down like a sock. Does anyone know } where one can buy these anymore? } } Thank you, } } Lita Duraine } } Login Host: 128.249.1.202 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- }
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*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
âââ On december 2015 1-4 2nd Practical Workshop on Advanced Microscopy -at- www.nic.iit.it Confirmed speakers: Sara Abrahamsson, Martin Oheim, Silvia Galiani, Chiara Cordiglieri, Colin JR Sheppard, Laura Cancedda, Luca Lanzano, Gail McConnell First day: open access talks (14-19) at Sala delle Grida, Genoa City Center, De Ferrari metro station. Following days: practicals, only 30 seats available at Nikon Imaging Center at IIT, Morego. NO registration fee. Mandatory regsitration for Practicals, no fee, applicants served on a first in first out basis. Visit also www.nic.iit.it for possible updates.
Registrations: email to alberto.diaspro-at-iit.it AND lauretta.galeno-at-iit.it - subject (mandatory â2nd NIC Workshop 2015â)
Final program link: http://www.nic.iit.it/wp-content/uploads/2015/11/r-Flyer-2nd-NIC-IIT-14NOV2015.pdf -----------
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Good evening folks, Iâm looking for a replacement collimator for a Thermo EDX detector, model number 5515E-1SUS-SN. Mine seems to have misplaced itself and I cannot find it anywhere. Would anyone have a spare they might part with, or have a lead as to where I might find a replacement?
Thanks!
David Hobart, Ph.D.c Visiting Professor in Chemistry 117 Surge Building Virginia Tech Department of Chemistry Blacksburg, VA 24061 dhobart-at-vt.edu ph. (540) 231-6837
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You may find that the dissolution is more thorough and faster to do this in two stages, where the first involves some fresh Canadian balsam dissolved in solvent and the second employs fresh solvent alone. Although counter-intuitive, this is often the fastest way to remove aged or thermally degraded resins by pulling the solubility parameters of the solvent closer to those of the target material. This also might allow you to get effective cleaning without recourse to xylene, using a solvent such as ethanol or isopropanol with fewer health issues.
John Twilley Conservation Scientist
-----Original Message----- } From: zaluzec-at-microscopy.com } Sent: Nov 13, 2015 4:05 AM } To: jtwilley-at-sprynet.com } Subject: [Microscopy] viaWWW:Canada Balsam on old glass slide } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 5, 26 -- From jtwilley-at-sprynet.com Mon Nov 16 01:25:26 2015 5, 26 -- Received: from elasmtp-kukur.atl.sa.earthlink.net (elasmtp-kukur.atl.sa.earthlink.net [209.86.89.65]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAG7PQN9013345 5, 26 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Nov 2015 01:25:26 -0600 5, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 26 -- s=dk20050327; d=sprynet.com; 5, 26 -- b=Hp3xlqlGP9MBw+ZA5BkO4dbU0lCbT2AKMrWBUgDCDl1+rqp5kLU0borQcDRAnfnM; 5, 26 -- h=Message-ID:Date:From:Reply-To:To:Subject:Mime-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-ELNK-Trace:X-Originating-IP; 5, 26 -- Received: from [209.86.224.33] (helo=elwamui-darkeyed.atl.sa.earthlink.net) 5, 26 -- by elasmtp-kukur.atl.sa.earthlink.net with esmtpa (Exim 4.67) 5, 26 -- (envelope-from {jtwilley-at-sprynet.com} ) 5, 26 -- id 1ZyEA5-0007ih-TF 5, 26 -- for Microscopy-at-Microscopy.com; Mon, 16 Nov 2015 02:25:13 -0500 5, 26 -- Received: from 24.44.53.206 by webmail.earthlink.net with HTTP; Mon, 16 Nov 2015 02:25:13 -0500 5, 26 -- Message-ID: {4061551.1447658713966.JavaMail.root-at-elwamui-darkeyed.atl.sa.earthlink.net} 5, 26 -- Date: Mon, 16 Nov 2015 02:25:13 -0500 (GMT-05:00) 5, 26 -- From: {jtwilley-at-sprynet.com} 5, 26 -- Reply-To: jtwilley-at-sprynet.com 5, 26 -- To: Microscopy-at-Microscopy.com 5, 26 -- Subject: Re: [Microscopy] viaWWW:Canada Balsam on old glass slide 5, 26 -- Mime-Version: 1.0 5, 26 -- Content-Type: text/plain; charset=UTF-8 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- X-Mailer: EarthLink Zoo Mail 1.0 5, 26 -- X-ELNK-Trace: adbb89a220ca650b5741bb2dafe8270574d3f0892ff6d3ed207985a50f233b3ec200193bdd6653b5350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 5, 26 -- X-Originating-IP: 209.86.224.33 ==============================End of - Headers==============================
We have done several conventional TEM processings of Staph aureus bacteria. We are getting moon shaped bacteria which we presume to be an artifact given that these are healthy, wild type control bacteria that should be round. If you would like a picture, please let me know and I can send one.
Roughly outlined our processing protocol is: - when bacteria are at appropriate stage (as defined by research group) the group fixes the bacteria in their lab with a PFA / Glut fixation in phosphate buffer (overnight, in fridge) - pellet bacteria, rinse out fixative, add low melting point agarose that is just warm enough to be liquid, mix with bacteria - solidly agarose on ice, divide sample into small cubes while in phosphate buffer (to prevent sample from drying out) - stain with tannic acid, 1hr, room temp - post-fix with 1% Osmium tetroxide, 1 hr on ice - en-block stain with UA, 1hr, room temp, in the dark - dehydrate 30% 50%, 75% (overnight), 90%, 100% x 3 - infiltrate gradually into EPON, embed, into oven for 24 hrs for resin to harden
I would appreciate any help to identify what could cause this Moon shape. I suspect the artifact happens before the agarose step because the agarose in the background appears to follow the moon shape but perhaps there is some secret to processing gram positive bacteria that can easily explain this artifact.
I have also had discussions with these scientists about trying HPF / FS. If anyone has suggestions for the cocktail and the processing duration I would be most interested to know.
Thank-you again for your help Kind regards Erin
_________________________________ DrÂŞ Erin Tranfield Head of the Electron Microscopy Facility Instituto Gulbenkian de CiĂŞncia Rua da Quinta Grande, 6 2780-156 Oeiras, Portugal Tel: +351 21 446 4691 e-mail: etranfield-at-igc.gulbenkian.pt http://uic.igc.gulbenkian.pt/emf.php
==============================Original Headers============================== 9, 35 -- From etranfield-at-igc.gulbenkian.pt Mon Nov 16 04:25:35 2015 9, 35 -- Received: from mx00.igc.gulbenkian.pt (mx00.igc.gulbenkian.pt [193.137.94.10]) 9, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAGAPYYR008566 9, 35 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Nov 2015 04:25:34 -0600 9, 35 -- Received: from localhost (localhost [127.0.0.1]) 9, 35 -- by mx00.igc.gulbenkian.pt (Postfix) with ESMTP id 1BD292B60BF 9, 35 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Nov 2015 10:25:29 +0000 (WET) 9, 35 -- X-Virus-Scanned: Debian amavisd-new at igc.gulbenkian.pt 9, 35 -- Received: from mx00.igc.gulbenkian.pt ([127.0.0.1]) 9, 35 -- by localhost (igc.gulbenkian.pt [127.0.0.1]) (amavisd-new, port 10024) 9, 35 -- with LMTP id nD5LeokakIAo for {Microscopy-at-Microscopy.com} ; 9, 35 -- Mon, 16 Nov 2015 10:25:27 +0000 (WET) 9, 35 -- Received: from mx03 (mx03.igc.gulbenkian.pt [192.168.50.13]) 9, 35 -- by mx00.igc.gulbenkian.pt (Postfix) with ESMTP id 8BE1D2B60AA 9, 35 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Nov 2015 10:25:27 +0000 (WET) 9, 35 -- Received: from localhost (localhost [127.0.0.1]) 9, 35 -- by mx03 (Postfix) with ESMTP id 5D4D3621E7 9, 35 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Nov 2015 10:25:27 +0000 (WET) 9, 35 -- Received: from mx03 ([127.0.0.1]) 9, 35 -- by localhost (mx03.igc.gulbenkian.pt [127.0.0.1]) (amavisd-new, port 10024) 9, 35 -- with ESMTP id Whugpah5lKhz for {Microscopy-at-Microscopy.com} ; 9, 35 -- Mon, 16 Nov 2015 10:25:26 +0000 (WET) 9, 35 -- Received: from etranfieldmbp.igc.gulbenkian.pt (fw1dmz.igc.gulbenkian.pt [192.168.50.3]) 9, 35 -- by mx03 (Postfix) with ESMTPSA id 04B7261C9A 9, 35 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Nov 2015 10:25:25 +0000 (WET) 9, 35 -- From: Erin Tranfield {etranfield-at-igc.gulbenkian.pt} 9, 35 -- Content-Type: text/plain; charset=utf-8 9, 35 -- Subject: Conventional Processing of Gram Positive Bacteria 9, 35 -- Message-Id: {DF9BECD0-79A9-43D3-BB72-4D322564BF5B-at-igc.gulbenkian.pt} 9, 35 -- Date: Mon, 16 Nov 2015 10:25:25 +0000 9, 35 -- To: Microscopy-at-Microscopy.com 9, 35 -- Mime-Version: 1.0 (Mac OS X Mail 9.1 \(3096.5\)) 9, 35 -- X-Mailer: Apple Mail (2.3096.5) 9, 35 -- Content-Transfer-Encoding: 8bit 9, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tAGAPYYR008566 ==============================End of - Headers==============================
From improve.alexa.ranks-at-gmail.com Mon Nov 16 11:05:03 2015 Return-Path: {improve.alexa.ranks-at-gmail.com} Received: from gmail.com ([59.18.93.250]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAGH50f9009885 for {microscopylistserverarchive5-at-microscopy.com} ; Mon, 16 Nov 2015 11:05:01 -0600 Reply-To: improve.alexa.ranks-at-gmail.com
Hi fellow microscopists
One of our users is planning to look at x-section diameter of collagen fibril in human peridontal ligament. However, he cannot put the tissue into the usual mix aldehyde EM fixative right away.There is most likely a few hours in transit between tissue harvest and arrival at the lab. What would be a good compromise procedure? I can think of the following but your advice will be much appreciated.
1. Will ice cold buffered saline during transit slow down autolysis enough to get reasonable ultrastucture?
2. I found a Nature Protocols paper on the use of buffered formalin when EM fix is not available and it also recommend to transfer to glutaraldehyde as soon as possible. However, will the small amount of methanol in formalin affect the size of collagen fibrils?
Graham, Lesley, and Jan Marc Orenstein. âProcessing Tissue and Cells for Transmission Electron Microscopy in Diagnostic Pathology and Research.â Nature Protocols 2, no. 10 (October 2007): 2439â50. doi:10.1038/nprot.2007.304.
3. Freezing is probably a bad idea for ultrastructure preservation but will it affect the size of collagen fibrils?
Thanks a lot!
-- Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519) The Biology Imaging Facility (http://depts.washington.edu/if/)
==============================Original Headers============================== 9, 43 -- From wpchan-at-uw.edu Mon Nov 16 13:35:55 2015 9, 43 -- Received: from mail-ob0-f169.google.com (mail-ob0-f169.google.com [209.85.214.169]) 9, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAGJZsiJ012975 9, 43 -- for {microscopy-at-microscopy.com} ; Mon, 16 Nov 2015 13:35:55 -0600 9, 43 -- Received: by obbww6 with SMTP id ww6so129951872obb.0 9, 43 -- for {microscopy-at-microscopy.com} ; Mon, 16 Nov 2015 11:35:49 -0800 (PST) 9, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 43 -- d=uw_edu.20150623.gappssmtp.com; s=20150623; 9, 43 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 9, 43 -- :content-type:content-transfer-encoding; 9, 43 -- bh=h3FYt930x01TVTLom7NXa/wK7FAKvvcRDyM1T+3VUvI=; 9, 43 -- b=TaEnPJvcCe1i7gLLU08uTF1Z8fUGYysM6VQBFokWADbxhgjvY55WFX0GAkPMUR4Wev 9, 43 -- sGfnxkxqXXvpst49aV1UKthlpL7hIRjFI8ICk1eDE4TBWAmBbk/dM/7DPBFzZVSVsRM+ 9, 43 -- bAH0J97vXS7NmeUOWkLArJXxu4wgxcanqprebsLkyyh3bWufEj37hwEX53y6s5jWJIVF 9, 43 -- YAMsZJWj7O++FpGUSiO/fLFg8QxfgHFSNdhadgt2/sjis5VLqr5y7DF1Ag5dCRjw3Z7w 9, 43 -- Bhm5ep7QxgY50aRUTnYmbGNXSJVYZ7qKoeNQ65fRY7e/LdJ++74XTufKnfuAeyG1itS2 9, 43 -- pHSA== 9, 43 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 43 -- d=1e100.net; s=20130820; 9, 43 -- h=x-gm-message-state:mime-version:in-reply-to:references:date 9, 43 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 9, 43 -- bh=h3FYt930x01TVTLom7NXa/wK7FAKvvcRDyM1T+3VUvI=; 9, 43 -- b=E+crGu8KcZBNxJPh1D7cS0/OGPHSEiHyC2E1LW/ElEItIkWOHSJxqFrSJtfjubvqMl 9, 43 -- z0XglL/ua9/EBCT7phbeJrP9mWK9VKOkS8eVqCt945NA7p13N5FYwE/cBHMvhhncPA7s 9, 43 -- 2aain6Kju+fN6ZjUf19kREf92lbUZqrmSrc5pukag1aa7ortbfNK1tfQPf/aOmXIrz/t 9, 43 -- xBU96iTdpLOtKbbc03I2+Oc/gkjxWeel9LH/nhI4EUd8gU7k9WcOXQ1MG+k+4cQH4dOh 9, 43 -- ikF388iQO8YAA/69Ph8Uvv/40dbRUxLGSQPrIDDCVacYUZsP39xpVgzmyRnSQUEgBH0/ 9, 43 -- zZUA== 9, 43 -- X-Gm-Message-State: ALoCoQlCItXaUmQPQqbTwDs5KNHR3usd731RM7OndFvuNThV0Sm2chaGLV4I/zWLhaM/q2FOg7S+ 9, 43 -- MIME-Version: 1.0 9, 43 -- X-Received: by 10.182.66.8 with SMTP id b8mr22691033obt.53.1447702549587; Mon, 9, 43 -- 16 Nov 2015 11:35:49 -0800 (PST) 9, 43 -- Received: by 10.202.81.82 with HTTP; Mon, 16 Nov 2015 11:35:49 -0800 (PST) 9, 43 -- In-Reply-To: {CALbJ0i6Vnw8wvViXa34fxv8jFAXsZYuEDqXkzuXSYj7LpoqtsA-at-mail.gmail.com} 9, 43 -- References: {CALbJ0i6Vnw8wvViXa34fxv8jFAXsZYuEDqXkzuXSYj7LpoqtsA-at-mail.gmail.com} 9, 43 -- Date: Mon, 16 Nov 2015 11:35:49 -0800 9, 43 -- Message-ID: {CALbJ0i7RsuRmUe43jagSSoPMxMjD-i+3vxH+_Vg5erBn06gqOw-at-mail.gmail.com} 9, 43 -- Subject: Fwd: collagen fiber TEM prep 9, 43 -- From: Wai Pang Chan {wpchan-at-uw.edu} 9, 43 -- To: microscopy-at-microscopy.com 9, 43 -- Content-Type: text/plain; charset=UTF-8 9, 43 -- Content-Transfer-Encoding: 8bit 9, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tAGJZsiJ012975 ==============================End of - Headers==============================
I have am seeking a postdoctoral researcher to carry out studies in the area of electron channeling contrast imaging (ECCI). The postdoc will be responsible for performing ECCI characterization of dislocation morphologies in a wide range of materials, with an emphasis on nanoindentation structures. Qualified candidates should have strong backgrounds in SEM and TEM crystallographic analysis techniques, such as diffraction contrast TEM, electron backscattered diffraction (EBSD), or electron channeling, as well as experience with focused ion beam (FIB) sectioning. The duration of the position will be 1-2 years. Interested candidates should contact me directly via email at crimp-at-egr.msu.edu. Please share this information with anyone you think might have an interest in this position.
Martin Crimp, Professor Dept. Chemical Engineering and Materials Science Michigan State Universitymi East Lansing, MI 48823 (517) 355-0294
==============================Original Headers============================== 6, 23 -- From crimp-at-egr.msu.edu Mon Nov 16 14:30:29 2015 6, 23 -- Received: from mail.egr.msu.edu (boomhauer.egr.msu.edu [35.9.37.164]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAGKUTlo001776 6, 23 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Nov 2015 14:30:29 -0600 6, 23 -- Received: from boomhauer (localhost [127.0.0.1]) 6, 23 -- by mail.egr.msu.edu (Postfix) with ESMTP id 4137246587 6, 23 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Nov 2015 15:30:24 -0500 (EST) 6, 23 -- X-Virus-Scanned: amavisd-new at egr.msu.edu 6, 23 -- Received: from mail.egr.msu.edu ([127.0.0.1]) 6, 23 -- by boomhauer (boomhauer.egr.msu.edu [127.0.0.1]) (amavisd-new, port 10024) 6, 23 -- with ESMTP id U2YcjJ71jcrw for {Microscopy-at-microscopy.com} ; 6, 23 -- Mon, 16 Nov 2015 15:30:24 -0500 (EST) 6, 23 -- Received: from EGR authenticated sender crimp 6, 23 -- To: Microscopy-at-Microscopy.Com 6, 23 -- From: Martin Crimp {crimp-at-egr.msu.edu} 6, 23 -- Subject: Postdoctoral Position Available 6, 23 -- Message-ID: {564A3CDF.9010305-at-egr.msu.edu} 6, 23 -- Date: Mon, 16 Nov 2015 15:30:23 -0500 6, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.10; rv:38.0) 6, 23 -- Gecko/20100101 Thunderbird/38.3.0 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have worked with collagen (type 1) in teeth stored for months in a buffer or just saline before fixation, have not seen visible changes; banding pattern was good. But I have not measured diameter of fibers. Collagen is a tough thing, I believe (just believe...) delay of a few hours with fixation will not harm it.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: wpchan-at-uw.edu [mailto:wpchan-at-uw.edu] Sent: Monday, November 16, 2015 1:37 PM To: Dusevich, Vladimir
Hi fellow microscopists
One of our users is planning to look at x-section diameter of collagen fibril in human peridontal ligament. However, he cannot put the tissue into the usual mix aldehyde EM fixative right away.There is most likely a few hours in transit between tissue harvest and arrival at the lab. What would be a good compromise procedure? I can think of the following but your advice will be much appreciated.
1. Will ice cold buffered saline during transit slow down autolysis enough to get reasonable ultrastucture?
2. I found a Nature Protocols paper on the use of buffered formalin when EM fix is not available and it also recommend to transfer to glutaraldehyde as soon as possible. However, will the small amount of methanol in formalin affect the size of collagen fibrils?
Graham, Lesley, and Jan Marc Orenstein. âProcessing Tissue and Cells for Transmission Electron Microscopy in Diagnostic Pathology and Research.â Nature Protocols 2, no. 10 (October 2007): 2439â50. doi:10.1038/nprot.2007.304.
3. Freezing is probably a bad idea for ultrastructure preservation but will it affect the size of collagen fibrils?
Thanks a lot!
-- Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519) The Biology Imaging Facility (http://depts.washington.edu/if/)
==============================Original Headers============================== 9, 43 -- From wpchan-at-uw.edu Mon Nov 16 13:35:55 2015 9, 43 -- Received: from mail-ob0-f169.google.com (mail-ob0-f169.google.com [209.85.214.169]) 9, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAGJZsiJ012975 9, 43 -- for {microscopy-at-microscopy.com} ; Mon, 16 Nov 2015 13:35:55 -0600 9, 43 -- Received: by obbww6 with SMTP id ww6so129951872obb.0 9, 43 -- for {microscopy-at-microscopy.com} ; Mon, 16 Nov 2015 11:35:49 -0800 (PST) 9, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 43 -- d=uw_edu.20150623.gappssmtp.com; s=20150623; 9, 43 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 9, 43 -- :content-type:content-transfer-encoding; 9, 43 -- bh=h3FYt930x01TVTLom7NXa/wK7FAKvvcRDyM1T+3VUvI=; 9, 43 -- b=TaEnPJvcCe1i7gLLU08uTF1Z8fUGYysM6VQBFokWADbxhgjvY55WFX0GAkPMUR4Wev 9, 43 -- sGfnxkxqXXvpst49aV1UKthlpL7hIRjFI8ICk1eDE4TBWAmBbk/dM/7DPBFzZVSVsRM+ 9, 43 -- bAH0J97vXS7NmeUOWkLArJXxu4wgxcanqprebsLkyyh3bWufEj37hwEX53y6s5jWJIVF 9, 43 -- YAMsZJWj7O++FpGUSiO/fLFg8QxfgHFSNdhadgt2/sjis5VLqr5y7DF1Ag5dCRjw3Z7w 9, 43 -- Bhm5ep7QxgY50aRUTnYmbGNXSJVYZ7qKoeNQ65fRY7e/LdJ++74XTufKnfuAeyG1itS2 9, 43 -- pHSA== 9, 43 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 43 -- d=1e100.net; s=20130820; 9, 43 -- h=x-gm-message-state:mime-version:in-reply-to:references:date 9, 43 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 9, 43 -- bh=h3FYt930x01TVTLom7NXa/wK7FAKvvcRDyM1T+3VUvI=; 9, 43 -- b=E+crGu8KcZBNxJPh1D7cS0/OGPHSEiHyC2E1LW/ElEItIkWOHSJxqFrSJtfjubvqMl 9, 43 -- z0XglL/ua9/EBCT7phbeJrP9mWK9VKOkS8eVqCt945NA7p13N5FYwE/cBHMvhhncPA7s 9, 43 -- 2aain6Kju+fN6ZjUf19kREf92lbUZqrmSrc5pukag1aa7ortbfNK1tfQPf/aOmXIrz/t 9, 43 -- xBU96iTdpLOtKbbc03I2+Oc/gkjxWeel9LH/nhI4EUd8gU7k9WcOXQ1MG+k+4cQH4dOh 9, 43 -- ikF388iQO8YAA/69Ph8Uvv/40dbRUxLGSQPrIDDCVacYUZsP39xpVgzmyRnSQUEgBH0/ 9, 43 -- zZUA== 9, 43 -- X-Gm-Message-State: ALoCoQlCItXaUmQPQqbTwDs5KNHR3usd731RM7OndFvuNThV0Sm2chaGLV4I/zWLhaM/q2FOg7S+ 9, 43 -- MIME-Version: 1.0 9, 43 -- X-Received: by 10.182.66.8 with SMTP id b8mr22691033obt.53.1447702549587; Mon, 9, 43 -- 16 Nov 2015 11:35:49 -0800 (PST) 9, 43 -- Received: by 10.202.81.82 with HTTP; Mon, 16 Nov 2015 11:35:49 -0800 (PST) 9, 43 -- In-Reply-To: {CALbJ0i6Vnw8wvViXa34fxv8jFAXsZYuEDqXkzuXSYj7LpoqtsA-at-mail.gmail.com} 9, 43 -- References: {CALbJ0i6Vnw8wvViXa34fxv8jFAXsZYuEDqXkzuXSYj7LpoqtsA-at-mail.gmail.com} 9, 43 -- Date: Mon, 16 Nov 2015 11:35:49 -0800 9, 43 -- Message-ID: {CALbJ0i7RsuRmUe43jagSSoPMxMjD-i+3vxH+_Vg5erBn06gqOw-at-mail.gmail.com} 9, 43 -- Subject: Fwd: collagen fiber TEM prep 9, 43 -- From: Wai Pang Chan {wpchan-at-uw.edu} 9, 43 -- To: microscopy-at-microscopy.com 9, 43 -- Content-Type: text/plain; charset=UTF-8 9, 43 -- Content-Transfer-Encoding: 8bit 9, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tAGJZsiJ012975 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 30 -- From DusevichV-at-umkc.edu Mon Nov 16 14:37:02 2015 18, 30 -- Received: from mst-rip6-umkc-out.um.umsystem.edu (mst-rip6-umkc-out.um.umsystem.edu [198.209.50.152]) 18, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAGKb1gC005961 18, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Nov 2015 14:37:01 -0600 18, 30 -- X-IronPort-Anti-Spam-Filtered: true 18, 30 -- X-IronPort-Anti-Spam-Result: A2BoBQDHPUpW/9CeoM9aA4M7U28Gv3AEWRcKhW8CHIErPBABAQEBAQEBgQqENAEBAQQjERo3BAIBCBEDAQEBAwIGAhsDAgICLwEUAQgIAgQKCQgVBId4AxINql+LRg1GAYQQAQEBAQEBAQEBAQEBAQEBAQEBAQEBGIEBhVOEfoJ6AYFAAQEeBhALGQ2CGToTHIEVBYdEiyODYQGFHIllSZJbg1SDcjgrhARyAYNIOgGBBgEBAQ 18, 30 -- X-IPAS-Result: A2BoBQDHPUpW/9CeoM9aA4M7U28Gv3AEWRcKhW8CHIErPBABAQEBAQEBgQqENAEBAQQjERo3BAIBCBEDAQEBAwIGAhsDAgICLwEUAQgIAgQKCQgVBId4AxINql+LRg1GAYQQAQEBAQEBAQEBAQEBAQEBAQEBAQEBGIEBhVOEfoJ6AYFAAQEeBhALGQ2CGToTHIEVBYdEiyODYQGFHIllSZJbg1SDcjgrhARyAYNIOgGBBgEBAQ 18, 30 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) 18, 30 -- by mst-rip6-exch-relay.um.umsystem.edu with ESMTP; 16 Nov 2015 14:36:54 -0600 18, 30 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.205]) by 18, 30 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi id 14.03.0266.001; 18, 30 -- Mon, 16 Nov 2015 14:36:48 -0600 18, 30 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 18, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 18, 30 -- Subject: RE: [Microscopy] Fwd: collagen fiber TEM prep 18, 30 -- Thread-Topic: [Microscopy] Fwd: collagen fiber TEM prep 18, 30 -- Thread-Index: AQHRIKYf9HV6Wd7uIkGlmVawApTPhJ6fGb3Q 18, 30 -- Date: Mon, 16 Nov 2015 20:36:47 +0000 18, 30 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DCADA28-at-UM-MBX-T01.um.umsystem.edu} 18, 30 -- References: {201511161936.tAGJakB5013645-at-ns.microscopy.com} 18, 30 -- In-Reply-To: {201511161936.tAGJakB5013645-at-ns.microscopy.com} 18, 30 -- Accept-Language: en-US 18, 30 -- Content-Language: en-US 18, 30 -- X-MS-Has-Attach: 18, 30 -- X-MS-TNEF-Correlator: 18, 30 -- x-originating-ip: [134.193.156.38] 18, 30 -- Content-Type: text/plain; charset="utf-8" 18, 30 -- MIME-Version: 1.0 18, 30 -- Content-Transfer-Encoding: 8bit 18, 30 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id tAGKb1gC005961 ==============================End of - Headers==============================
From ohn.bullocknewsnorox-at-gmail.com Tue Nov 17 20:16:08 2015 Return-Path: {ohn.bullocknewsnorox-at-gmail.com} Received: from gmail.com ([121.78.112.171]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id tAI2G3po028124 for {microscopylistserverarchive5-at-microscopy.com} ; Tue, 17 Nov 2015 20:16:05 -0600 Message-ID: {C759E65D.E92F2771-at-gmail.com}
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X-from: calixtoa1988-at-gmail.com
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Title-Subject: [Filtered] Utilization of calibration standards
Message: Dear All,
We are trying to setup a formal QC system in our SEM lab. The issue I am facing is that I cannot find Âstablished protocols for calibrating the detectors. There are in the market some contrast standards, resolution standards, etc. but I is not clear to me how are they supposed to be used. Could someone here advise or point me where to look ?
Big Thanks
Calixto
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] Colorado State University Workshop: High Speed Imaging for In-situ TEM and STEM Diffraction
Message: If you are in the Colorado area and perform in-situ TEM and/or S/TEM diffraction, we invite you to attend this unique session featuring the latest high speed imaging technology and applications.
December 9 (8:30 a.m. Â 5:00 p.m.): Investigators will present and demonstrate recent developments in high speed digital cameras and applications for in-situ TEM and tomography. December 10 (8:30 a.m. Â 5:00 p.m.): We will discuss various methods using electron diffraction for crystallographic characterization of materials. This will include presentations on S/TEM diffraction techniques and live demonstrations of 4D-STEM diffraction applications using high performance cameras.
Daily registration will be from 8:45 Â 9:15 a.m. and will include coffee and doughnuts. Lectures start at 9:15 a.m. and lunch will be served daily. Registration, all lectures, and meals will be held in the Yates EM Lab, Room 102.
We also invite you to the concurrent Mountain States Society of Electron Microscopists & Colorado Microbeam Analysis Society Annual Fall Meeting at 6:00 Â 9:00 p.m., December 9, 2015. This is an informal social gathering of microscopists from local institutions and companies. Also remember, it is prime ski season in Colorado! So it is a great opportunity to enjoy a special microscopy event and the outdoors of Colorado. We hope to see you there!
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
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==============================Original Headers============================== 25, 43 -- From nestor.zaluzec-at-gmail.com Thu Nov 19 02:16:40 2015 25, 43 -- Received: from mail-wm0-f50.google.com (mail-wm0-f50.google.com [74.125.82.50]) 25, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAJ8Gd2Y018420; 25, 43 -- Thu, 19 Nov 2015 02:16:40 -0600 25, 43 -- Received: by wmvv187 with SMTP id v187so12836386wmv.1; 25, 43 -- Thu, 19 Nov 2015 00:16:34 -0800 (PST) 25, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 25, 43 -- d=gmail.com; s=20120113; 25, 43 -- h=sender:from:subject:references:cc:to:reply-to:message-id:date 25, 43 -- :user-agent:mime-version:in-reply-to:content-type 25, 43 -- :content-transfer-encoding; 25, 43 -- bh=Hro83uf6n2/3wfK4RQRhltXFa9L+At/3krWBaGLi1iA=; 25, 43 -- b=Rt7qMxCey3W6Zqgow8oK2LUW+w/soLbejrR7fmdxYxM5THluMhai/JgSkneh3feu/U 25, 43 -- n1iNbd/3l0ZZLJZcotKdTSbRExLPqQe3Fmpgi8KKlRNsatT34NYTP/bgUh2PiFaMQ0Vq 25, 43 -- YqwUiKq0fBwHMSrD2dPZEQULxDmS7/CDJLinnID3edvISVQJkaXPhOb6R7v1aANSjK0n 25, 43 -- T6gmaIR9pbXBYAWlm4zJYP/9Hap9THWqpVvZbsfA97MwCDrV6+OKUomdtnLLWEaQTWUt 25, 43 -- fLfEYS2u/7xvsjISeCatTLDy8mraEoFcRaWRtsVH53SuWSRkJE1oScC8fq94AQZDPBi9 25, 43 -- tQbg== 25, 43 -- X-Received: by 10.28.107.26 with SMTP id g26mr14797971wmc.34.1447920994472; 25, 43 -- Thu, 19 Nov 2015 00:16:34 -0800 (PST) 25, 43 -- Received: from dev192-150-183-104.eduroam.manchester.ac.uk ([192.150.183.104]) 25, 43 -- by smtp.googlemail.com with ESMTPSA id uz5sm6672657wjc.8.2015.11.19.00.16.33 25, 43 -- (version=TLSv1/SSLv3 cipher=OTHER); 25, 43 -- Thu, 19 Nov 2015 00:16:33 -0800 (PST) 25, 43 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 25, 43 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 25, 43 -- X-Google-Original-From: MicroscopyListserver-NoReply 25, 43 -- {microscopylistserver-noreply-at-microscopy.com} 25, 43 -- Subject: viaWWW:Colorado State University Workshop: High Speed Imaging for 25, 43 -- In-situ TEM and STEM Diffraction 25, 43 -- References: {201511171829.tAHITvaO010900-at-ns.microscopy.com} 25, 43 -- Cc: zaluzec-at-microscopy.com 25, 43 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 25, 43 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 25, 43 -- X-Forwarded-Message-Id: {201511171829.tAHITvaO010900-at-ns.microscopy.com} 25, 43 -- Message-ID: {564D8560.9020508-at-microscopy.com} 25, 43 -- Date: Thu, 19 Nov 2015 08:16:32 +0000 25, 43 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 25, 43 -- Gecko/20100101 Thunderbird/38.3.0 25, 43 -- MIME-Version: 1.0 25, 43 -- In-Reply-To: {201511171829.tAHITvaO010900-at-ns.microscopy.com} 25, 43 -- Content-Type: text/plain; charset=UTF-8; format=flowed 25, 43 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Filtered] Diamond Knife woes!
Message: Hi All in microscopy land,
Lately we have had a hard time getting our Diatome diamond knives re-sharpened correctly from Diatome. We usually send sets of three at a time for the re-sharpening service. The last three times though, we have had to send back our knives after waiting for three months, because of either epoxy problems in the boat, nicks still in the knives, or some kind of equidistant tooling marks. We have even tried exchanging the knives for new ones, but once they need sharpening again, some of these problems start all over again.
Have any of you had the same problems and if so, are there any other companies out there that can offer better sharpening service...hopefully without buying all new knives? Any experience with Ted Pella?
I appreciate it,
Lita Duraine
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May I suggest you get in touch with Helmut Gnaegi from Diatome. He is not only extremely knowledgeable but also very very helpful and takes great pride in the quality of Diatome knives. I am certain almost everyone here on the Listserver would agree.
His email address is helmut.gnaegi-at-diatome.ch.
I have no commercial interest in Diatome (unfortunatelyâŚ).
Good luck,
Jan Leunissen Aurion
Dunedin, New Zealand.
==============================Original Headers============================== 7, 31 -- From leunissen-at-aurion.nl Thu Nov 19 13:53:02 2015 7, 31 -- Received: from smtp102.tnz.mail.aue.yahoo.com (smtp102.tnz.mail.aue.yahoo.com [124.108.96.71]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAJJr0d9021883 7, 31 -- for {microscopy-at-microscopy.com} ; Thu, 19 Nov 2015 13:53:01 -0600 7, 31 -- Received: (qmail 74601 invoked from network); 19 Nov 2015 19:52:55 -0000 7, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1447962775; bh=S08ht4DYtenNhZYOQfjYGsP4gjR/oEDBzpCR89VTycI=; h=Subject:Mime-Version:Content-Type:From:In-Reply-To:Date:Cc:Content-Transfer-Encoding:Message-Id:References:To; b=2jmWENpihyWZT6XMtxaMK9cCyeinHtOnmlBefV5ml/onH55lh+iWxqfLtdiIuy2jqk2UmZ0GFuPQczeiE7zeXf+UweCVKB7XNdhfnVjKRb7fYuhye8qSniejN2olqaHHEKdlodNbZjR78eR6+mYrlfPcNVbS/Ge7h9IBzIFoWj8= 7, 31 -- X-Yahoo-Newman-Property: ymail-3 7, 31 -- X-YMail-OSG: eShM.zsVM1lugt4HLcUe534L.3kNRlYQmpyBye4eN6UKOp8 7, 31 -- 28ebcbMPYPLcJu3ptxVFkoodf_QawN0WO7D9L_ZMIYvUZW.WVn2MmrDm7KXR 7, 31 -- iyBCTbn4gDoGzEYvau9WNLGJ6rnbjv.1PmJOOvBH_1XREcN4y2mwnzFXNxAf 7, 31 -- wd05LfySNY_KA6LCy299tUw6Zvgo15Abop6WVEEkIAZkJKoESH.2ZQD_MSbx 7, 31 -- 1UQtk0vOhTh5o9MBX804PUhizknHadyngkgVK5R.EFX.ytw3R3CagAkcZ9zN 7, 31 -- zHxNnEkcEaqb67_RFDCklaygrTZrzmVJlGjdOW5hVYuD0e1LdUyq.u3.KWC6 7, 31 -- 1k1FtqrUO9iT.gWnEieCA75ASQdlJm1iE38mCla8ruef0O26hwgXbk93j.k_ 7, 31 -- wYrEw05vmvdHfspW3rXtJ.b.0aUqFvRVeCuPdZ8G6GmhHaNLZEUIC5Aiblk3 7, 31 -- U0Mehxqp65aoLz2hyo2khx0Ba1MTK.H.1B2U65MrfjgH_awZaZ.g3W2FvJEf 7, 31 -- ZqNvqd9oTB1DzvWmkMCFcxpgJOquZYmXYMXy0Tw-- 7, 31 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 7, 31 -- Subject: Re: [Microscopy] viaWWW:Diamond Knife woes! 7, 31 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 7, 31 -- Content-Type: text/plain; charset=utf-8 7, 31 -- From: Jan Leunissen {leunissen-at-aurion.nl} 7, 31 -- In-Reply-To: {201511190821.tAJ8Lneq020967-at-ns.microscopy.com} 7, 31 -- Date: Fri, 20 Nov 2015 08:52:52 +1300 7, 31 -- Cc: microscopy-at-microscopy.com 7, 31 -- Message-Id: {5A3E2492-CDC9-4578-A5B8-CC1B6536CF5F-at-aurion.nl} 7, 31 -- References: {201511190821.tAJ8Lneq020967-at-ns.microscopy.com} 7, 31 -- To: duraine-at-bcm.edu 7, 31 -- X-Mailer: Apple Mail (2.2104) 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tAJJr0d9021883 ==============================End of - Headers==============================
I was wondering if anyone on this list has the manual(s) for a FEI FIB 800 Focused Ion Beam system.
I'm specifically looking for the part that describes the GIS ports and the part with the technical details including size and dimensions of the (1) columns assembly, (2) the table and (3) the rack.
Thank you and best regards, Stefan
==============================Original Headers============================== 4, 26 -- From dev.c0debabe-at-gmail.com Mon Nov 23 10:34:09 2015 4, 26 -- Received: from mail.tbmn.org (mail.tbmn.org [87.118.84.39]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tANGY74K007379 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Nov 2015 10:34:07 -0600 4, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 4, 26 -- by mail.tbmn.org (Postfix) with ESMTP id 4859A276C37F 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Nov 2015 17:34:01 +0100 (CET) 4, 26 -- Received: from mail.tbmn.org ([127.0.0.1]) 4, 26 -- by localhost (mail.tbmn.org [127.0.0.1]) (amavisd-new, port 10024) 4, 26 -- with ESMTP id g-7QmS6F77AU for {Microscopy-at-microscopy.com} ; 4, 26 -- Mon, 23 Nov 2015 17:34:01 +0100 (CET) 4, 26 -- Received: from [192.168.1.5] (chello080108008029.35.11.vie.surfer.at [80.108.8.29]) 4, 26 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 4, 26 -- (No client certificate requested) 4, 26 -- (Authenticated sender: mne-at-tbmn.org) 4, 26 -- by mail.tbmn.org (Postfix) with ESMTPSA id 0C5F5276C0BD 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Nov 2015 17:34:01 +0100 (CET) 4, 26 -- Message-ID: {56533FF3.1040502-at-gmail.com} 4, 26 -- Date: Mon, 23 Nov 2015 17:33:55 +0100 4, 26 -- From: Stefan Schoenleitner {dev.c0debabe-at-gmail.com} 4, 26 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:31.0) Gecko/20100101 Thunderbird/31.7.0 4, 26 -- MIME-Version: 1.0 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Subject: FEI FIB 800 Manual 4, 26 -- Content-Type: text/plain; charset=utf-8 4, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From infoirlee-at-asia.com Wed Nov 25 04:41:53 2015 Return-Path: {infoirlee-at-asia.com} Received: from srv01.radlink.com.br (ns1.radlink.com.br [189.50.192.6] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tAPAfq4s020468; Wed, 25 Nov 2015 04:41:52 -0600 Message-Id: {201511251041.tAPAfq4s020468-at-ns.microscopy.com} Received: from 75-145-207-221-busname-va.richmond.hfc.comcast.net ([75.145.207.221] helo=User) by srv01.radlink.com.br with esmtpa (Exim 4.72) (envelope-from {infoirlee-at-asia.com} ) id 1a1XQU-0004VM-FB; Wed, 25 Nov 2015 08:35:50 -0200 Reply-To: {irleeshingsee-at-gmail.com}
X-from: cvierret-at-mst.edu
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Email: cvierret-at-mst.edu Name: Clarissa Wisner
Organization: Missouri University of Science and Technology
Title-Subject: [Filtered] 4Pi
Message: We have had a 4Pi system on our Hitachi S570 and it stopped working about two months ago. We have been trying to get it up and running but we have not had much success. We have been in touch with the previous owners of 4Pi and are working on alternatives. At this point we are asking if any one in the microscopy community has a 4Pi box that they are no longer using that could be acquired in a minimal charge. Please respond to me directly, cvierret-at-mst.edu, as I rarely check the list server.
Thanks Clarissa
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Title-Subject: [Filtered] Microscope camera resolution
Message: When collecting images for creating a (stitched) mosaic of a sample (thin section or polished block), are there reasons for using a high resolution camera with a low magnification objective as opposed to a low resolution camera with a high magnification objective? Are there general principles that apply to all cameras or is it too specific, depending on the camera's sensor, phototube optics, etc.? I would expect there to be reasons for keeping it simple and not using pixel-shifting cameras but is it sensible to keep it simpler still and use the lowest resolution (i.e. cheapest) camera and highest magnification objective? Conversely, if we have already bought a camera, is it wasting its capabilities to be using it at lower than maximum resolution, or might we be getting a better image? If this is a well-known problem that has been discussed in a textbook then I apologise for wasting the list's time and will happily buy the book. Thank you for any responses, Barrie
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Email: tjk210-at-lehigh.edu Name: Tia Kowal
Organization: Lehigh University
Title-Subject: [Filtered] Quantomix capsules
Message: Hi there. I'm wondering if anyone has any experience using the Quantomix WETSEM chambers. I'm considering using them for an experiment, but since they are kind of expensive, wanted to get some input before purchasing them. I don't need to use them for very high magnification. Any info on good or bad experiences would be appreciated.
Thank you!
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Email: dongsheng.li2-at-pnnl.gov Name: Dongsheng Li
Organization: PNNL
Title-Subject: [Filtered] Postdoc position available
Message: I like to hire a postdoc. Please see the details below.
Thanks.
Post Doctorate RA - In situ TEM study of branched nanocrystal growth mechanisms: Job Description
Fundamental research of this project is focused on understanding the mechanisms and kinetics of vapor-liquid-solid (VLS) and solution synthesis. Synthesis methods will involve use of mainly VLS route (templating agents and bio-inspired processing in solution may also be included) to achieve size and shape controllable synthesis across scales. In situ techniques (such as TEM) are the principal technique that will be used to investigate nucleation and self-assembly and advance the understanding of the relationship between surface structure/chemistry, pathways of formation, and materials properties. This position requires expertise in the synthesis and structural characterization of nanostructured materials derived from VLS techniques.
Excellent oral and written communications skills are mandatory. Other duties will include publication of results in peer-reviewed journals, technical presentations at scientific conferences, and development of proposals for new research projects. The candidate must be able to independently design and carry out experiments as well as function productively as part of a multidisciplinary team.
Minimum Requirements Candidates must have received a PhD degree within the past five years from an accredited college or university. All staff at the Pacific Northwest National Laboratory must be able to demonstrate the legal right to work in the United States.
Other Qualifications  Ph.D. in physics, chemistry, materials science, or related field  Background in experimental physics, chemical physics, or materials science  Expertise in TEM imaging, elemental analysis, and diffraction pattern analysis  Experience with EDS, EELS, XRD analysis  Knowledge of Molecular dynamics and Density functional theory (optional)  Experience with AFM-based imaging, (optional)  Experience working with solution-based nanoparticle systems  Strong analytical skills  Knowledge of Crystallography  Record of excellence in research  Demonstrated capability to think and act independently  Excellent written and oral communication skills  Ability to collaborate
Contact: Dr. Dongsheng Li, Dongsheng.li2-at-pnnl.gov
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Title-Subject: [Filtered] Systems for Billing - IdeaElan
Message: Happy Thanksgiving Eve,
Has anyone implemented a core management system from the company IdeaElan? If so, would you be willing to talk to me and a few of my colleagues about your experience with this company? They offer billing software, work-flow management and equipment scheduling.
With thanks,
Lesley
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From hanja07656515151zr-at-gmail.com Thu Nov 26 20:38:48 2015 Return-Path: {hanja07656515151zr-at-gmail.com} Received: from gmail.com (122-116-99-133.HINET-IP.hinet.net [122.116.99.133]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id tAR2cjqU004718 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 26 Nov 2015 20:38:46 -0600 Message-ID: {0D5FBC15.A796804F-at-gmail.com}
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Michigan Technological University has an opening for a Research Specialist in scanning transmission electron microscopy S(TEM).
The person hired for this position will be required to manage and operate a FEI Titan Themis (S)TEM and associated GIF and EDS. Instrument operation will be for users from multiple disciplines across campus and external to campus. In addition to managing the laboratory, responsibilities will include assisting in the design of experiments utilizing imaging and spectroscopic functions as well as utilization of multiple in-situ holders. The person will be required to stimulate the use of the equipment through educational outreach both on and off campus and investigate the inclusion of the scope capabilities in new research efforts. The minimum requirements for the position are a PhD in engineering or physical science and 3 years operational experience with the same or similar equipment to FEI Themis Titan (S)TEM with EELS/EDS. The successful candidate will have a working knowledge of the electron microscopy principles/physics and enthusiasm for multidisciplinary application of the (S)TEM and related spectroscopy techniques.
A full job description and details for application are found at https://www.jobs.mtu.edu/postings/3657
Michigan Technological University is an Equal Opportunity Educational Institution/Equal Opportunity Employer, which includes providing equal opportunity for protected veterans and individuals with disabilities.
-- Steve Hackney, Professor Dept. Materials Science and Engineering MM 501 Michigan Technological University 1400 Townsend, Houghton MI 49931 906 487 2170
-- Owen P Mills Director, Applied Chemical and Morphological Analysis Laboratory Michigan Technological University 1400 Townsend Dr Houghton, MI 49931 906-369-1875 http://mcff.mtu.edu/acmal/
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X-from: patrick.smith-at-beg.utexas.edu
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Email: patrick.smith-at-beg.utexas.edu Name: patrick
Organization: University of texas - austin
Title-Subject: [Filtered] TEM Beam energy on sample calculation
Message: We,re working with complex geologic samples and would like to calculate the current(dose)on sample at a given Kev. Also the beam diameter.
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==============================Original Headers============================== 13, 40 -- From microscopy.listserver-at-gmail.com Tue Dec 1 19:20:40 2015 13, 40 -- Received: from mail-qk0-f173.google.com (mail-qk0-f173.google.com [209.85.220.173]) 13, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tB21Ked0008363 13, 40 -- for {microscopy-at-microscopy.com} ; Tue, 1 Dec 2015 19:20:40 -0600 13, 40 -- Received: by qkas77 with SMTP id s77so10598268qka.0 13, 40 -- for {microscopy-at-microscopy.com} ; Tue, 01 Dec 2015 17:20:34 -0800 (PST) 13, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 40 -- d=gmail.com; s=20120113; 13, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 13, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 13, 40 -- bh=QFiR8YHz7Q8SfqzTMcYwTiKmFylSFmmV1qDN/0Lv/+8=; 13, 40 -- b=Z1nbDtbVHTJWdKUQawq3HRIc3VY2SpBdjRYcUVpTDGgZuK6ZAoGTQQcyLwHf1t/bGq 13, 40 -- WUbvjDxMF536C2bMcsjnx6Rv+otu+1AyFkiA3DzBDyZW8OZkqZs+wPckdIt3rEFhXkWf 13, 40 -- qonTphdjNfBvCgwJpJZhtagJwzONbrV7ALiPtJtzIm0i52+rX79/fXUoipXC04LcCb4n 13, 40 -- 0ITSqM1nhtmKhMVIoNMavG6ci6O1fX6BQUEeAfq14X28POgpi1LQIEjNwghn/kDOQ/++ 13, 40 -- Zqe5OVrW7+qBXD7ZGQ3Zyms/xTrd641uKnvonB6X/8HN9FpXKgdL6Qg2W4qpuOkgvXRS 13, 40 -- gHmA== 13, 40 -- X-Received: by 10.55.215.76 with SMTP id m73mr536654qki.16.1449019234566; 13, 40 -- Tue, 01 Dec 2015 17:20:34 -0800 (PST) 13, 40 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1]) 13, 40 -- by smtp.googlemail.com with ESMTPSA id c140sm240900qkb.30.2015.12.01.17.20.33 13, 40 -- for {microscopy-at-microscopy.com} 13, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 13, 40 -- Tue, 01 Dec 2015 17:20:34 -0800 (PST) 13, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 13, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 13, 40 -- {microscopylistserver-noreply-at-microscopy.com} 13, 40 -- Subject: viaWWW:TEM Beam energy on sample calculation 13, 40 -- References: {201512011521.tB1FLFpI009257-at-ns.microscopy.com} 13, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 40 -- X-Forwarded-Message-Id: {201512011521.tB1FLFpI009257-at-ns.microscopy.com} 13, 40 -- Message-ID: {565E4761.90802-at-microscopy.com} 13, 40 -- Date: Tue, 1 Dec 2015 19:20:33 -0600 13, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 13, 40 -- Gecko/20100101 Thunderbird/38.3.0 13, 40 -- MIME-Version: 1.0 13, 40 -- In-Reply-To: {201512011521.tB1FLFpI009257-at-ns.microscopy.com} 13, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello Colorado Area Microscopists and Microanalysts
INVITED SPEAKERS Dr. Andreas Hoenger, University of Colorado "Cryo-EM studies on Microtubule-MAP interactions in vitro and in situ"
Dr. Ai Leen Koh, Stanford University "In Situ and Environmental High Resolution Electron Microscopy of Material Reactions"
WHEN & WHERE Wednesday December 9, 2015 from 6:00 PM to 9:00 PM CB & Potts (on Collindale Golf Course) -- southwest style buffet and cash bar 1441 East Horsetooth Road Fort Collins, CO 80525 Carpool opportunities available from west Denver area
RSVP by December 6 to hlowers-at-usgs.gov $30 nonmember $20 MSSEM/CMAS member $10 student
Please send payment to and make check out to MSSEM/CMAS C/O John Chandler 423 Buckeye St. Fort Collins, CO 80524
In addition to the meeting,Gatan and JEOL are hosting a free workshop December 9 and 10 from 9:00 AM to 5:00 PM at Colorado State University. Wednesday's focus will be on in-situ EM with heating and tomography and Thursday's will be on STEM diffraction imaging.
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9, 75 -- Wed, 02 Dec 2015 08:20:33 -0800 (PST) 9, 75 -- MIME-Version: 1.0 9, 75 -- X-Received: by 10.31.169.8 with SMTP id s8mr2507523vke.148.1449073232650; Wed, 9, 75 -- 02 Dec 2015 08:20:32 -0800 (PST) 9, 75 -- Received: by 10.31.94.66 with HTTP; Wed, 2 Dec 2015 08:20:32 -0800 (PST) 9, 75 -- Date: Wed, 2 Dec 2015 09:20:32 -0700 9, 75 -- Message-ID: {CAOx2-aCSqX=RA=-iTxwncCebV1-93kdNg=MiCBnRudJdjFXBoQ-at-mail.gmail.com} 9, 75 -- Subject: Local Colorado Microscopy Meeting and Gatan/JEOL Workshop 9, 75 -- From: "Lowers, Heather" {hlowers-at-usgs.gov} 9, 75 -- To: microscopy {Microscopy-at-microscopy.com} 9, 75 -- Content-Type: text/plain; charset="UTF-8" 9, 75 -- X-Gm-Spam: 0 9, 75 -- X-Gm-Phishy: 0 9, 75 -- X-CFilter-Loop: Reflected 9, 75 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFnrGLMWRmVeSWpSXmKPExsXCxfTjjW6EVHyYQesbFos/hxvYHRg9Pna/ 9, 75 -- ZgpgjOKySUnNySxLLdK3S+DKmDb9AnPBDM6KbwumMTUw7mHvYuTiYBHYwirx+u88xi5GDg4J 9, 75 -- AROJP1+ATE4gU0ziwr31bCA1QgJNTBIvtj5jBUkICaxmlGh/bAyRmMoo8f7LJyYQR0JgJqvE 9, 75 -- g9k/2CGcH4wS6z4cYYWYVSExY9VSZhCbV0BQ4uTMJywQ8UKJb70r2CHGeki0LvwCVs8ioCLR 9, 75 -- fWQjI0R9gMS3d2vZQGxhASeJF1f+M4HYbAJaEpNPHwOLiwDZbb37wOYwC2hKtG7/zQ4xn0di 9, 75 -- ytyLUDafxJorW1ggdslK9E3ogbpBUuLgihssExjFZiE5bxaSUQsYmVYxChXnlhSY6hXnphfp 9, 75 -- peRn6qXnl21iBIU+lwz3DsZfDywOMQpwMCrx8AZwxYUJsSaWFVfmHmKU4GBWEuH15okPE+JN 9, 75 -- SaysSi3Kjy8qzUktPsQozcGiJM6br+IUJiSQnliSmp2aWpBaBJNl4uCUamDsvcIa/iku/MAH 9, 75 -- L7/iCNuDebn3/XWPdX569CsnVT7IWnR9jCvPxJtcJ04qJiiuKbeZV5MguiT2fM8/xSomBtGV 9, 75 -- GoGXwtc8DX3KqdFpscVN5s9kAe4HRw7/WG7zTzeh93y00+qcrt6+gn2CIdxTd8Rv7jDgDLgv 9, 75 -- WuzBkm2rd9dnqa/Urv27hZRYijMSDbWYi4oTAdW5kZ15AgAA ==============================End of - Headers==============================
From advertisebz09ojzy-at-gmail.com Wed Dec 2 12:15:25 2015 Return-Path: {advertisebz09ojzy-at-gmail.com} Received: from gmail.com ([221.162.19.200]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id tB2IFMa5017068 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 2 Dec 2015 12:15:23 -0600 Message-ID: {668A9400.3F69D32A-at-gmail.com}
Listers, Apologies for cross posting on the confocal list.
Bindley Bioscience Center is seeking an Imaging Facility Manager to contribute to establishing and to lead the Imaging Core in Purdueâs Discovery Park. The position involves establishing collaborations with faculty, staff and students to assist with biological imaging research and develop projects. Develop and implement new methodologies for routine and specialized use within the scope of scientific image capture and analysis from a variety of imaging modalities, principally in fluorescence imaging ranging from wide-field, to confocal and super-resolution scale. Other technologies include SPECT/CT, as well as other multiple modality whole animal imaging. To see the full posting and to apply, please visit: http://purdue.taleo.net/careersection/wl/jobdetail.ftl?job=1500877&lang=en
You may contact me for informal enquiries
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu
==============================Original Headers============================== 7, 30 -- From gilpin-at-purdue.edu Wed Dec 2 16:14:43 2015 7, 30 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tB2MEhTf028297 7, 30 -- for {microscopy-at-microscopy.com} ; Wed, 2 Dec 2015 16:14:43 -0600 7, 30 -- Received: from WPVEXCHUB03.purdue.lcl (wpvexchub03.itap.purdue.edu [172.30.136.67]) 7, 30 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id tB2MEbYR002069 7, 30 -- for {microscopy-at-microscopy.com} ; Wed, 2 Dec 2015 17:14:38 -0500 7, 30 -- Received: from WPVEXCMBX04.purdue.lcl ([169.254.4.141]) by 7, 30 -- WPVEXCHUB03.purdue.lcl ([172.30.136.67]) with mapi id 14.03.0224.002; Wed, 2 7, 30 -- Dec 2015 17:14:37 -0500 7, 30 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 7, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 30 -- Subject: Imaging Facility Manager position at Purdue 7, 30 -- Thread-Topic: Imaging Facility Manager position at Purdue 7, 30 -- Thread-Index: AQHRLU7VL8khJ7rphEq11gKoQtQ/mw== 7, 30 -- Date: Wed, 2 Dec 2015 22:14:37 +0000 7, 30 -- Message-ID: {F68CEFA2-D5B5-49FF-8E79-6452B2F74452-at-purdue.edu} 7, 30 -- Accept-Language: en-US 7, 30 -- Content-Language: en-US 7, 30 -- X-MS-Has-Attach: 7, 30 -- X-MS-TNEF-Correlator: 7, 30 -- x-originating-ip: [10.163.23.192] 7, 30 -- Content-Type: text/plain; charset="utf-8" 7, 30 -- Content-ID: {145C6751C1C47A42BFFDDF9CCD47D997-at-exchange.purdue.edu} 7, 30 -- MIME-Version: 1.0 7, 30 -- X-PMX-Version: 6.0.2.2308539 7, 30 -- X-PerlMx-URL-Scanned: Yes 7, 30 -- X-PerlMx-Virus-Scanned: Yes 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id tB2MEhTf028297 ==============================End of - Headers==============================
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM Workshop at Georgia USA
Message: The Georgia Electron Microscopy center will be having a Biological TEM workshop March 9-11, 2016. These are all day workshops designed to train anyone (students, research staff, post-docs, faculty, etc...) on basic TEM preparation of biological samples and TEM operation. The workshop will be held in Barrow Hall on the University of Georgia Athens campus.
Registration is limited to six people and registration is through iLabs (link at our website - gem.uga.edu) Deadline is Feb 26.
For more information and a schedule of the workshop, visit gem.uga.edu or contact John Shields (jpshield-at-uga.edu)
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Email: jm-at-tamu.edu Name: Ji Ma
Organization: Department of Materials Science and Engineering, Texas A&M University
Title-Subject: [Filtered] Position for research scientist in Transmission Electron Microscopy
Message: Research Scientist Position in Transmission Electron Microscopy Department of Materials Science and Engineering Texas A&M University
The Department of Materials Science and Engineering is seeking a research scientist with expertise in Transmission Electron Microscopy (TEM), with special focus on high-resolution imaging and diffraction techniques in crystalline solids. The research scientist will work with faculty and students within the department to carry out TEM imaging and characterization of primarily on metals and ceramics, interpret and analyze the results, and author or co-author scientific publications. The candidate will also supervise and mentor students on the fundamentals of TEM technique, sample preparation, and assist them in the interpretation of results. Qualified candidates will be involved in proposal development and writing activities.
Qualification and Experience - Ph.D. in materials science, metallurgy, or related fields is preferred - Strong background and expertise in electron diffraction of crystalline solids and high resolution imaging is required - Experience in electron back-scattered diffraction (EBSD) and atom probe tomography (APT) is not required but encouraged - Strong record of scientific publications is required
The Department of Materials Science and Engineering currently has 14 full time faculty, 4 faculty with non-zero joint appointments, and 45 affiliated faculty from several departments. The number of graduate students is currently about 140, most of whom are pursuing Ph.D.s. The department is fast growing and the target for the department is to increase the number of full-time faculty to more than 20 and establish an undergraduate program in the next three years; and increase to 30 faculty, with 750 undergraduate and graduate students by 2025.
Applicants should submit a cover letter, curriculum vitae, and a list of three references (including postal addresses, phone numbers and email addresses) at the following website:
https://www.tamengineeringjobs.com/postings/2200
For questions regarding the position, please contact: Dr. Ji Ma Department of Materials Science and Engineering Texas A&M University TAMU 3003 College Station, TX 77843-3003 E-mail: jm-at-tamu.edu
Full consideration will be given to applications received by Jan. 3, 2016.
The Texas A&M University System is affirmative action/equal opportunity employer dedicated to the goal of building a culturally diverse and pluralistic faculty and staff committed to teaching and working in a multicultural environment. We strongly encourage applications from women, minorities, individuals with disabilities, and covered veterans.
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==============================Original Headers============================== 24, 41 -- From microscopy.listserver-at-gmail.com Wed Dec 2 23:21:30 2015 24, 41 -- Received: from mail-io0-f170.google.com (mail-io0-f170.google.com [209.85.223.170]) 24, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tB35LMnU009206 24, 41 -- for {microscopy-at-microscopy.com} ; Wed, 2 Dec 2015 23:21:29 -0600 24, 41 -- Received: by ioc74 with SMTP id 74so69873866ioc.2 24, 41 -- for {microscopy-at-microscopy.com} ; Wed, 02 Dec 2015 21:21:17 -0800 (PST) 24, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 24, 41 -- d=gmail.com; s=20120113; 24, 41 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 24, 41 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 24, 41 -- bh=FxYrIujroHk7D4e7kAoeiAxQbbIrGw0tUd40a7zBu3A=; 24, 41 -- b=TRaGN/N67M3gu9AMD4iextFBaE7PdCTZJwj69p2g1BHGq+O1x5n98PiJQqDIRzYirG 24, 41 -- KQfBaUcXSqa3MFONvuQFQVc7suGZ9EeJocKaSvcpaLALaN97E+yYTjK0qffdyY3EnYjE 24, 41 -- TdspzWVZpKdAiefiPP9MZmwfnagUlAZi6xZVUqbV4O2EXeDRofFS8LbHCfz1dEkNZt8m 24, 41 -- TCuRwqaAF09ALee/M/17i1jyCvYRT1TUQcCZFFucmFLtZX4bRQYh1RZK+wlWEm1fcSB8 24, 41 -- f0Sw2LI3OmAvI6AAGLf1KoRZvI/WRjQPPZyVlm4FiygiVI4LQ2xe6g7Js7uyB0p5Hzjl 24, 41 -- DEwg== 24, 41 -- X-Received: by 10.107.134.166 with SMTP id q38mr7328170ioi.25.1449120076902; 24, 41 -- Wed, 02 Dec 2015 21:21:16 -0800 (PST) 24, 41 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 24, 41 -- by smtp.googlemail.com with ESMTPSA id l41sm2468865iod.34.2015.12.02.21.21.16 24, 41 -- for {microscopy-at-microscopy.com} 24, 41 -- (version=TLSv1/SSLv3 cipher=OTHER); 24, 41 -- Wed, 02 Dec 2015 21:21:16 -0800 (PST) 24, 41 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 24, 41 -- X-Google-Original-From: MicroscopyListserver-NoReply 24, 41 -- {microscopylistserver-noreply-at-microscopy.com} 24, 41 -- Subject: viaWWW:Position for research scientist in Transmission Electron 24, 41 -- Microscopy 24, 41 -- References: {201512022109.tB2L9isU025546-at-ns.microscopy.com} 24, 41 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 24, 41 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 24, 41 -- X-Forwarded-Message-Id: {201512022109.tB2L9isU025546-at-ns.microscopy.com} 24, 41 -- Message-ID: {565FD14B.4080900-at-microscopy.com} 24, 41 -- Date: Wed, 2 Dec 2015 23:21:15 -0600 24, 41 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 24, 41 -- Gecko/20100101 Thunderbird/38.3.0 24, 41 -- MIME-Version: 1.0 24, 41 -- In-Reply-To: {201512022109.tB2L9isU025546-at-ns.microscopy.com} 24, 41 -- Content-Type: text/plain; charset=windows-1252; format=flowed 24, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Is it possible to purchase ready-to-use uranyle and lead salts for contrasting of ultrathin sections in TEM? (this is for Austria so I would also need the name of the distributor in Austria) Many thanks in advance.
Regards, Stephane
==============================Original Headers============================== 3, 29 -- From nizets2-at-yahoo.com Fri Dec 4 03:17:53 2015 3, 29 -- Received: from nm21.bullet.mail.bf1.yahoo.com (nm21.bullet.mail.bf1.yahoo.com [98.139.212.180]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tB49Hrtg031081 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 4 Dec 2015 03:17:53 -0600 3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1449220667; bh=IygYAtZi3af0CAvKEjvSdtFFrQAq+E3xE39pN+NNqFY=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=U16fhh09vBNfPDS1JT4xrfNEWnZ/NW1EWqJFlZvhYxTFgvUpM+XnLLg2gMyjFdYDD/uEzSv6tL9sASomgPyrMc8HjT+JZqO09t8X66B6t4S8cuIYObxD5XtnEavU/Ry34XAvqLzPyjx7GPMdLvv3BF19OT7N3HQ+5/9ErSDP2WK9A0giia2G27lIfEMaZWUOy7KX91jmHB6dGGNjsm8XuuDeIfy7BUvmjDa364XT92UqQ0Hy94W8NKLq6ArG/OdDL9gRjDnAvJJjdZ+mYRIaBeidLvTk7f2PareIIv0YmCCJp5p5PwhJvCAbPX9zBRqBhHNpjIiKkwHCuFzXsN2jag== 3, 29 -- Received: from [98.139.170.179] by nm21.bullet.mail.bf1.yahoo.com with NNFMP; 04 Dec 2015 09:17:47 -0000 3, 29 -- Received: from [98.139.215.253] by tm22.bullet.mail.bf1.yahoo.com with NNFMP; 04 Dec 2015 09:17:47 -0000 3, 29 -- Received: from [127.0.0.1] by omp1066.mail.bf1.yahoo.com with NNFMP; 04 Dec 2015 09:17:47 -0000 3, 29 -- X-Yahoo-Newman-Property: ymail-3 3, 29 -- X-Yahoo-Newman-Id: 651964.56596.bm-at-omp1066.mail.bf1.yahoo.com 3, 29 -- X-YMail-OSG: DMXXzYwVM1nrQND_bh87EItryUWMm9BGkaXJHIbB7vpdDhpZxg0ZGWPRX.Od80T 3, 29 -- QgDU8uSTg1gwZKZcWavPxLWIHi6RAuTPF_kTI32HaeVyhPWSPGFfU0d_dIJwW3SvUrOtDlclVNqc 3, 29 -- .14cEYE6vw1fd_rwHBY3wotgg.pmtiP.H3q6mGpH4jjX92M4sAwxmhvE.g9BvAZSdMH3vj6RECzW 3, 29 -- 1uGytFHQPDiGilytRPIPxp.HFrh7Th6.b9dVy9L9f_3T19kyNamerGsn_HXW7jZc1AFjUF5OypDt 3, 29 -- Jhz1JvabG20_fRZmGn724NzyOV1AK.0svFJGwdbi4znb9iugX3Eqlzyq4HXOyztCxs38f03byrvZ 3, 29 -- 5_1JEFZ2F02jMR8eFBNOf20_9SYOcNWmzH8yB2NHXuGrOmyF8VKsf6F_pVdrpPWZZzmC2Oic5GVA 3, 29 -- YLUdgT7j0YHLX8f5UUZhfYE8RRCoGMM8PF5c4tu9rawQ_fU6gjqRkh.NkMbrbL4XeCEZxnXWgYCy 3, 29 -- OxpMX 3, 29 -- Received: by 76.13.26.64; Fri, 04 Dec 2015 09:17:47 +0000 3, 29 -- Date: Fri, 4 Dec 2015 09:17:46 +0000 (UTC) 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- To: {microscopy-at-microscopy.com} 3, 29 -- Message-ID: {1955991994.15233203.1449220666618.JavaMail.yahoo-at-mail.yahoo.com} 3, 29 -- Subject: Ready-to-use contrasting solutions 3, 29 -- MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=UTF-8 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- References: {1955991994.15233203.1449220666618.JavaMail.yahoo.ref-at-mail.yahoo.com} ==============================End of - Headers==============================
Hi Stephane, } ... readyâtoâuse uranyle and lead salts for contrasting of ultrathin sections in TEM?
Yes, in Germany, this is available. For Austria: you may ask the well known companies? I can see your point to ask ... in some cases, this might be advantageous. But, honestly, preparation of UAc solutions is straight forward, without any problem; and any premade solution is MUCH more expensive than buying the solid substance and making the stock solution yourself; buying a stock UAc = waste of money. For Lead acetate / citrate whatsoever: this might be a slightly different story, although I know there are plenty of good recipes in many websites / on many computers of kind colleagues, which are 'easy-to-do'. -- But, we found that for many samples, after good sample prep (mostly FS, now), and digital imaging on good sensitive cameras, you may not need Lead staining, at all ... the question is whether medium or dark grey is sufficient or if you really need black. just my two cents .... Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: - 16th Europ Microsc Congress EMC http://www.emc2016.fr/en/ 28 Aug - 2 Sept 2016 in Lyon, FR - Microscopy Conference 2017 Dreiländertagung Lausanne, CH 20-25 August 2017 - next Microbiol. conferences: VAAM - Annual Conf March 13-16, 2016, Jena
==============================Original Headers============================== 5, 22 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Fri Dec 4 03:37:11 2015 5, 22 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tB49bBCb005796 5, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Dec 2015 03:37:11 -0600 5, 22 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 5, 22 -- by localhost (Postfix) with SMTP id B0E7F60BCE 5, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Dec 2015 10:37:04 +0100 (CET) 5, 22 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 5, 22 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id 8A1D760A52 5, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Dec 2015 10:37:04 +0100 (CET) 5, 22 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 5, 22 -- with Novell_GroupWise; Fri, 04 Dec 2015 10:37:04 +0100 5, 22 -- Message-Id: {56616CCE0200005400059ACF-at-gwsmtp1.uni-regensburg.de} 5, 22 -- X-Mailer: Novell GroupWise Internet Agent 14.2.0 5, 22 -- Date: Fri, 04 Dec 2015 10:37:02 +0100 5, 22 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 5, 22 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 5, 22 -- Subject: staining solutions 5, 22 -- Mime-Version: 1.0 5, 22 -- Content-Type: text/plain; charset=UTF-8 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- Content-Disposition: inline ==============================End of - Headers==============================
I am seconding the first reply by Reinhard Rahel to this post from Stephane Nizet (Vienna, Austria).
Stephane, if you are in need for Austrian sources (specific EM-suppliers, knowing there are only VERY few!) I can provide their addresses and communication data if necessary. I know that often we had to order goods also by direct selling companies from Germany.
I would like to point to the problem of {legal purchase} of "radioactive" stuff in the European Community even/especially for electron microscopists and the fact that perhaps the quality of UO2Ac-powder will be a bit less than say 10 years ago (when there was introduced a limit of storage volumes of "radioactive natural (depleted) uranium" and biggest packages of 10g UO2Ac.powder were sold... rising problem in using the hygroscopic powder).
The only problem I had over the years (1% methanolic UO2Acetate + a drop of concentrated acetic acid {for better nuclear staining} as the originally traditional recipe told) was more/less rapid precipitation of yellow needles and also patchy deposit of (perhaps) U-(hydroxide?, UO4.4H2O?) despite careful handling, storing in critically cleaned glassware and shielding the solution from light anytime. This meant - making 25-50 ml of 1% methanolic UO2acetate stock = working solution - that sometimes I had to dispose of safely and compliant with serious legislation more than a quarter or sometimes a half of the ready-to-use solution. This drawback/problem I think I was able to overcome by admixing enough concentrated acetic acid (analog to an "equilibrium function" in/during radiolysis and formation of precipitates when using U-nitrate solutions, there exists a paper in Nature 200,671-672, 1963, doi:10.1038/200671a0) which I was pointed to by a personal MSA-Listserver communication [2012-01-22] from/by our famous colleague John J. Bozzola [TEPLY&STULIK, 1963_Nature 200_Formation of Peroxidic Precipitates in Radiolysis of Uranyl Nitrate Ketone Solutions ] perhaps analog also valid for UOAc-solutions using acetic acid??]
If anyone would like to get the pdf of that 1963-Nature article (and / or the recipes as well as disposal strategy I used in staining ultrathins), please request it and I shall send it....
Ready to use UO2acetate-solutions as well as Lead containing (citrate Reynolds or Venable-Coggeshall-like) staining solutions are available from LEICA (because they sell these for use in their automated ULTROstainer (aqueous solutions of uranyl acetate and lead citrate (Leica Ultrostain I and II)...
The making of UO2Ac as well as Lead-Citrate solutions (especially the latter after Venable&Coggeshall) is not difficult provided you are allowed to make yourself (EU-legislation and radiation safety! you must have absolved successfully one or 2 special courses and written test to become a certified "radiation (safety) officer" or the like to be allowed to i) store some grams of UO2-Acetate in your lab, and ii) to handle UO2-acetate (or also -nitrate) crystals from the pack to make the solution yourself.
At this time I have to announce /to tell you that since 1st of December 2015 I am {retiree} with only a short limited time to act from my desk at } work {.
I would like to thank all of you and especially those who not only were getting to know me better but also respected me as person but with all my inadequacies, mistakes, faults and errors I certainly made sometimes.
All those colleagues already having replied earlier and wished me all the best in the future: Again Thank YOU so much for your courtesy and continuing friendship which I really appreciate.
I wish you all a pleasant and successful time in healthiness, always {radiating happiness} .
It was a pleasure and really an honor to having been in the number of long standing MSA-Listserver- {fellows} and it might be that I shall/will be able to comment on one or the other matter in the future (if changing my delivery address for the MSA-listserver-messages will turn out to be successful).
God bless, cordially yours
Wolfgang
OR Dr. phil. Wolfgang Muss* Retired Head of EM-Lab Univ. Institute of Pathology, SALK-LKH (Salzburger Landeskliniken gemeinnuetzige GesmbH, Landeskrankenhaus = Federal State General Hosp.) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE *Honorary Secretary of SCUR (Society for Cutaneous Ultrastructure Research): see Announcements below*
former Member of Works Council SALK-LKH & Central Works Council SALK former Safety Counselor SALK-LKH, Certified Radiation Safety Officer
Web(German): http://ww.salk.at Web(German): http://www.pmu.ac.at Phone work: +43+662+4482+4720 Mobile phone work: +43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W. Muss") E-Mail work: W.Muss-at-SALK.at Private Address: Ignaz-Rieder-Kai 19/6, A-5020 SALZBURG, AUSTRIA Mobile-phone private: ++43+676+5 369-456 E-Mail private: wij.muss-at-aon.at
*)registered in: www.researchgate.net as "Wolfgang MUSS" (URL -at-: http://www.researchgate.net/profile/Wolfgang_MUSS/ ) [NB.: Registration and saving all your publications, posters, datasets etc. (free of charges) into your personal profile] ResearchGATE is the leading professional network for scientists. It's free of charge and designed to meet researchers' needs More than 7,000,000 scientists from over 193 countries have already joined! Join 7 million researchers, including 45 Nobel Laureates
* The SCUR-Secretary*: Information on behalf of Society for Cutaneous Ultrastructure Research (SCUR) - The SKIN IMAGING Society Visit the WEBSITE of SCUR - The Skin Imaging Society at } http://www.scur.org { ------------------------------------------------------------------------- 2016 The 43rd Ann. SCUR meeting will take place during the first 2 days of the 16th European Microscopy Congress - EMC2016 (http://www.emc2016.fr/en/), 29th-30th August 2016 in LYON, France. i.e., Monday afternoon from 2 PM to 7 PM and Tuesday whole day. We expect some 30 oral presentations and 3 guest lectures + posters.
*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+ =================================================================================================================== Von: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Gesendet: Freitag, 04. Dezember 2015 10:31 An: Muß Wolfgang Betreff: [Microscopy] Ready-to-use contrasting solutions
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Dear colleagues,
Is it possible to purchase ready-to-use uranyle and lead salts for contrasting of ultrathin sections in TEM? (this is for Austria so I would also need the name of the distributor in Austria) Many thanks in advance.
Regards, Stephane
==============================Original Headers============================== 3, 29 -- From nizets2-at-yahoo.com Fri Dec 4 03:17:53 2015 3, 29 -- Received: from nm21.bullet.mail.bf1.yahoo.com (nm21.bullet.mail.bf1.yahoo.com [98.139.212.180]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tB49Hrtg031081 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 4 Dec 2015 03:17:53 -0600 3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1449220667; bh=IygYAtZi3af0CAvKEjvSdtFFrQAq+E3xE39pN+NNqFY=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=U16fhh09vBNfPDS1JT4xrfNEWnZ/NW1EWqJFlZvhYxTFgvUpM+XnLLg2gMyjFdYDD/uEzSv6tL9sASomgPyrMc8HjT+JZqO09t8X66B6t4S8cuIYObxD5XtnEavU/Ry34XAvqLzPyjx7GPMdLvv3BF19OT7N3HQ+5/9ErSDP2WK9A0giia2G27lIfEMaZWUOy7KX91jmHB6dGGNjsm8XuuDeIfy7BUvmjDa364XT92UqQ0Hy94W8NKLq6ArG/OdDL9gRjDnAvJJjdZ+mYRIaBeidLvTk7f2PareIIv0YmCCJp5p5PwhJvCAbPX9zBRqBhHNpjIiKkwHCuFzXsN2jag== 3, 29 -- Received: from [98.139.170.179] by nm21.bullet.mail.bf1.yahoo.com with NNFMP; 04 Dec 2015 09:17:47 -0000 3, 29 -- Received: from [98.139.215.253] by tm22.bullet.mail.bf1.yahoo.com with NNFMP; 04 Dec 2015 09:17:47 -0000 3, 29 -- Received: from [127.0.0.1] by omp1066.mail.bf1.yahoo.com with NNFMP; 04 Dec 2015 09:17:47 -0000 3, 29 -- X-Yahoo-Newman-Property: ymail-3 3, 29 -- X-Yahoo-Newman-Id: 651964.56596.bm-at-omp1066.mail.bf1.yahoo.com 3, 29 -- X-YMail-OSG: DMXXzYwVM1nrQND_bh87EItryUWMm9BGkaXJHIbB7vpdDhpZxg0ZGWPRX.Od80T 3, 29 -- QgDU8uSTg1gwZKZcWavPxLWIHi6RAuTPF_kTI32HaeVyhPWSPGFfU0d_dIJwW3SvUrOtDlclVNqc 3, 29 -- .14cEYE6vw1fd_rwHBY3wotgg.pmtiP.H3q6mGpH4jjX92M4sAwxmhvE.g9BvAZSdMH3vj6RECzW 3, 29 -- 1uGytFHQPDiGilytRPIPxp.HFrh7Th6.b9dVy9L9f_3T19kyNamerGsn_HXW7jZc1AFjUF5OypDt 3, 29 -- Jhz1JvabG20_fRZmGn724NzyOV1AK.0svFJGwdbi4znb9iugX3Eqlzyq4HXOyztCxs38f03byrvZ 3, 29 -- 5_1JEFZ2F02jMR8eFBNOf20_9SYOcNWmzH8yB2NHXuGrOmyF8VKsf6F_pVdrpPWZZzmC2Oic5GVA 3, 29 -- YLUdgT7j0YHLX8f5UUZhfYE8RRCoGMM8PF5c4tu9rawQ_fU6gjqRkh.NkMbrbL4XeCEZxnXWgYCy 3, 29 -- OxpMX 3, 29 -- Received: by 76.13.26.64; Fri, 04 Dec 2015 09:17:47 +0000 3, 29 -- Date: Fri, 4 Dec 2015 09:17:46 +0000 (UTC) 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- To: {microscopy-at-microscopy.com} 3, 29 -- Message-ID: {1955991994.15233203.1449220666618.JavaMail.yahoo-at-mail.yahoo.com} 3, 29 -- Subject: Ready-to-use contrasting solutions 3, 29 -- MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=UTF-8 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- References: {1955991994.15233203.1449220666618.JavaMail.yahoo.ref-at-mail.yahoo.com} ==============================End of - Headers==============================
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Email: m_raven-at-lifesci.ucsb.edu Name: Mary Raven
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Email: kevinksl-at-att.net Name: Kevin Smith
Organization: KSL
Title-Subject: [Filtered] TEM
Message:
Im looking for a working TEM, or TEM that was known to be operational when disassemble. The instrument will be used to preform bright field and SAED analysis on environmental samples.
Im most familiar with JEOL, but have also worked with Philips instruments.
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Email: kun.li-at-kaust.edu.sa Name: Kun Li
Organization: King Abdullah University of Science and Technology (KAUST)
Title-Subject: [Filtered] Senior/TEM service engineer opening
Message: The Imaging and Characterization Core Lab of KAUST is equipped with state-of-the-art transmission electron microscopes, five of which are Titan. The EM facility is currently under a 3-year comprehensive service contract with FEI, and we are looking for an experienced senior/TEM service engineer for the smooth operation of the EM suite.
The main duties of the candidate include  Responsible for the overall maintenance of all the transmission electron microscopes in the Imaging and Characterization Core Lab to ensure high equipment uptime.  Conduct preventive maintenance of all the TEMs per predefined PM schedule.  Conduct trouble-shooting and corrective maintenance of all the TEMs.  Work with FEI factory service support team to speed up the trouble shooting and problem solving process.  Manage all the TEM spare parts and consumables.  Responsible for the management and maintenance of the TEM Sample Preparation Lab.
KAUST provides very competitive package and good living environment (www.kaust.edu.sa). If you are interested, please contact
kun.li-at-kaust.edu.sa Imaging and Characterization Core Lab Director
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Email: dbecker-at-bergen.org Name: David Becker
Organization: Bergen County Technical Schools
Title-Subject: [Filtered] Seeking Electron Microscopist
Message: We are looking for someone with electron microscopy experience to join our Nano Structural Imaging Laboratory, (NSIL), at Bergen Academies (Hackensack, NJ). We have TEM, SEM (Dual-Beam), EDX, Confocal along with a wide range of sample prep equipment. If you, or someone you know has experience in any, or all of the above we would be interested to hear from you\them. There is a formal job description and application procedure at http://tinyurl.com/ov3zul6 Thank You for passing this along!
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Tue Dec 8 18:05:12 2015 14, 40 -- Received: from mail-io0-f169.google.com (mail-io0-f169.google.com [209.85.223.169]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tB905BYP030680 14, 40 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2015 18:05:11 -0600 14, 40 -- Received: by iouu10 with SMTP id u10so42897788iou.0 14, 40 -- for {microscopy-at-microscopy.com} ; Tue, 08 Dec 2015 16:05:04 -0800 (PST) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=G/va+sjg67xSyOfqfvRcEv46UgdZu2eZVCsTXCOOR9Q=; 14, 40 -- b=qbCM4HCnmjNN9TkxHDMGK1hoK+xqiU+2EqVv7tnUrJyyPOJtcf0KCmrxDaz3ewVFW0 14, 40 -- 7rCzCkyhDmu8HPtgrfm8mqacD7Ez8sbfzVtUWBBNxEpdyHofjtWARjjQMsb1XPVyNtrR 14, 40 -- xbCcaniWeh8rirTO0QTjNNmh9VSJKOBbgkmM7ZYjOoNYQZUZhrj1SBzee0N3UwrD+oZI 14, 40 -- I01jmX3BjulgJEn2u0oe24Zrcd7VNwLYP/LLke6AoFXTqUbmvmbqBF7RtcjYCHJnNEnA 14, 40 -- AfkmAZWZTFIYBIInxdqGK0FT7NOK9usVYMso4u6s7UHA3UcnDk90E+R4stoZdgFJ8RwI 14, 40 -- MaPg== 14, 40 -- X-Received: by 10.107.186.3 with SMTP id k3mr3249291iof.43.1449619504454; 14, 40 -- Tue, 08 Dec 2015 16:05:04 -0800 (PST) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id g88sm2330365ioj.23.2015.12.08.16.05.03 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 14, 40 -- Tue, 08 Dec 2015 16:05:03 -0800 (PST) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:Seeking Electron Microscopist 14, 40 -- References: {201512081512.tB8FCSsO017793-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201512081512.tB8FCSsO017793-at-ns.microscopy.com} 14, 40 -- Message-ID: {5667702E.6070503-at-microscopy.com} 14, 40 -- Date: Tue, 8 Dec 2015 18:05:02 -0600 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.4.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201512081512.tB8FCSsO017793-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jm-at-tamu.edu Name: Ji Ma
Organization: Department of Materials Science and Engineering, Texas A&M University
Title-Subject: [Filtered] Position for research scientist in Transmission Electron Microscopy
Message: Research Scientist in Transmission Electron Microscopy Department of Materials Science and Engineering Texas A&M University
The Department of Materials Science and Engineering is seeking a research scientist with expertise in Transmission Electron Microscopy (TEM), with special focus on high-resolution imaging and diffraction techniques in crystalline solids. The research scientist will work with faculty and students within the department to carry out TEM imaging and characterization of primarily on metals and ceramics, interpret and analyze the results, and author or co-author scientific publications. The candidate will also supervise and mentor students on the fundamentals of TEM technique, sample preparation, and assist them in the interpretation of results. Qualified candidates will be involved in proposal development and writing activities.
Qualification and Experience: Ph.D. in materials science, metallurgy, or related fields is preferred.
Strong background and expertise in electron diffraction of crystalline solids and high resolution imaging is required.
Experience in electron back-scattered diffraction (EBSD) and atom probe tomography (APT) is not required but encouraged.
Strong record of scientific publications is required.
Applicants should submit a cover letter, curriculum vitae, and a list of three references (including postal addresses, phone numbers and email addresses) at the following website: https://www.tamengineeringjobs.com/postings/2200
For questions, please contact Dr. Ji Ma E-mail: jm-at-tamu.edu
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Title-Subject: [Filtered] Job position with FEI in Shangai
Message: Product Marketing Engineer  Based in Shangai
The Mission
The market division within FEI provides solutions that add quantifiable economic value in well-defined commercial applications encompassing process control, accelerated product design, and application specific analysis. We develop and sell well defined solutions based on FEI's core platforms and technologies. The Market Division is an entrepreneurial group within FEI heavily engaged in new market development.
The Position
The Product Marketing Engineer is responsible for developing business plans, marketing strategy, and forecasts for assigned product lines, maintaining a strong understanding of customer technical requirements for existing and future products. This position identifies, evaluates, and recommends marketing opportunities consistent with product line objectives.
Responsibilities and deliverables include:  Accompany sales engineers on customer visits to determine competitive strengths and weaknesses, assist sales force in the promotion of FEI products at trade shows  Provide feedback from existing customers, prospects, and the sales force to assist in the definition of product configurations or enhancements that target specific markets or market segments  Work with applications development to turn special customer requirements into saleable products or features  Develop sales support materials including brochures, applications notes, product data sheets, competitive matrices, and technical presentations  Develop and present technical papers at professional symposia, author technical articles for trade press  Develop and present training programs to the sales force to inform them of new products, enhancements, or competitive issues  Recommend, evaluate, and document FEI and third party accessories  Work with demonstration lab staff to develop effective specialized demonstration techniques
The Requirements
This position is ideal for an experienced professional wanting to be involved with a dynamic team and exposed to a constant variety of customer application areas. The successful candidate will possess the following combination of education and experience:  Typically requires a University degree in Material Science, Physics or a similar discipline, advanced degree preferred  Typically requires 10 years experience in a relevant technical environment  Experience with TEMs.  Ability to work in a team environment  Excellent, enthusiastic, clear communication skills with a diverse audience is critical to the success of this position  Ability to travel both domestically as well as internationally and possession of a valid passport
If you are interested please apply via the following link: https://global-fei.icims.com/jobs/5867/product-marketing-engineer/job All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, national origin, age, disability, veteran status, marital status, pregnancy, gender expression or identity, sexual orientation, or any other legally-protected status.
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==============================Original Headers============================== 21, 40 -- From microscopy.listserver-at-gmail.com Thu Dec 10 06:54:01 2015 21, 40 -- Received: from mail-io0-f179.google.com (mail-io0-f179.google.com [209.85.223.179]) 21, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBACs15n018520 21, 40 -- for {microscopy-at-microscopy.com} ; Thu, 10 Dec 2015 06:54:01 -0600 21, 40 -- Received: by ioir85 with SMTP id r85so90951860ioi.1 21, 40 -- for {microscopy-at-microscopy.com} ; Thu, 10 Dec 2015 04:53:53 -0800 (PST) 21, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 21, 40 -- d=gmail.com; s=20120113; 21, 40 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 21, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 21, 40 -- bh=PcoXS2JV+2Z8N+Czw/vMEkqtfv+a5XnAiUABRzk3sTg=; 21, 40 -- b=UyrjtKQ6377wrMspkYI57o76VMQTFagtfuG/goJFRaQOoyFbAcsTXcRWSXWi/FZmFE 21, 40 -- R5EmcfPTdgR6q08X7zSBw2x8767tGa+oOXyxIEhCoCBxHvMd4b0PlWMHBrMqNPQiL884 21, 40 -- +1o0SYhLVVrBfKVMc+jPvwYA3cU/e3bptoA+GsKmqSJHcLHweL5caMhJhkeCFBVn11Ec 21, 40 -- F3zTLSmi9zjNV1uTjTcIX5I3lk+Q++3TgO2xAcLxYujWkcJNn7fRYBHQAIgnkUG/6OG9 21, 40 -- 69Ui7HHKyUF9Ce1jw11Ja8uZ+9D9WrX2xxQ6ixkn+cRwPw9Fr/37db5uFeyFdtWfVnnz 21, 40 -- 4/MQ== 21, 40 -- X-Received: by 10.107.151.131 with SMTP id z125mr11082481iod.192.1449752033625; 21, 40 -- Thu, 10 Dec 2015 04:53:53 -0800 (PST) 21, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 21, 40 -- by smtp.googlemail.com with ESMTPSA id p79sm5014162ioi.15.2015.12.10.04.53.52 21, 40 -- for {microscopy-at-microscopy.com} 21, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 21, 40 -- Thu, 10 Dec 2015 04:53:53 -0800 (PST) 21, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 21, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 21, 40 -- {microscopylistserver-noreply-at-microscopy.com} 21, 40 -- Subject: viaWWW: 21, 40 -- References: {201512100416.tBA4GIoh011205-at-ns.microscopy.com} 21, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 40 -- X-Forwarded-Message-Id: {201512100416.tBA4GIoh011205-at-ns.microscopy.com} 21, 40 -- Message-ID: {566975E0.6060104-at-microscopy.com} 21, 40 -- Date: Thu, 10 Dec 2015 06:53:52 -0600 21, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 21, 40 -- Gecko/20100101 Thunderbird/38.4.0 21, 40 -- MIME-Version: 1.0 21, 40 -- In-Reply-To: {201512100416.tBA4GIoh011205-at-ns.microscopy.com} 21, 40 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 40 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Jasmijn.van.den.Borne-at-fei.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Job position with FEI in Shangai
Message: Product Marketing Engineer  Based in Shangai
The Mission
The market division within FEI provides solutions that add quantifiable economic value in well-defined commercial applications encompassing process control, accelerated product design, and application specific analysis. We develop and sell well defined solutions based on FEI's core platforms and technologies. The Market Division is an entrepreneurial group within FEI heavily engaged in new market development.
The Position
The Product Marketing Engineer is responsible for developing business plans, marketing strategy, and forecasts for assigned product lines, maintaining a strong understanding of customer technical requirements for existing and future products. This position identifies, evaluates, and recommends marketing opportunities consistent with product line objectives.
Responsibilities and deliverables include:  Accompany sales engineers on customer visits to determine competitive strengths and weaknesses, assist sales force in the promotion of FEI products at trade shows  Provide feedback from existing customers, prospects, and the sales force to assist in the definition of product configurations or enhancements that target specific markets or market segments  Work with applications development to turn special customer requirements into saleable products or features  Develop sales support materials including brochures, applications notes, product data sheets, competitive matrices, and technical presentations  Develop and present technical papers at professional symposia, author technical articles for trade press  Develop and present training programs to the sales force to inform them of new products, enhancements, or competitive issues  Recommend, evaluate, and document FEI and third party accessories  Work with demonstration lab staff to develop effective specialized demonstration techniques
The Requirements
This position is ideal for an experienced professional wanting to be involved with a dynamic team and exposed to a constant variety of customer application areas. The successful candidate will possess the following combination of education and experience:  Typically requires a University degree in Material Science, Physics or a similar discipline, advanced degree preferred  Typically requires 10 years experience in a relevant technical environment  Experience with TEMs.  Ability to work in a team environment  Excellent, enthusiastic, clear communication skills with a diverse audience is critical to the success of this position  Ability to travel both domestically as well as internationally and possession of a valid passport
If you are interested please apply via the following link: https://global-fei.icims.com/jobs/5867/product-marketing-engineer/job All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, national origin, age, disability, veteran status, marital status, pregnancy, gender expression or identity, sexual orientation, or any other legally-protected status.
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==============================Original Headers============================== 21, 39 -- From microscopy.listserver-at-gmail.com Thu Dec 10 07:51:34 2015 21, 39 -- Received: from mail-qg0-f53.google.com (mail-qg0-f53.google.com [209.85.192.53]) 21, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBADpYL8010986 21, 39 -- for {microscopy-at-microscopy.com} ; Thu, 10 Dec 2015 07:51:34 -0600 21, 39 -- Received: by qgeb1 with SMTP id b1so143085638qge.1 21, 39 -- for {microscopy-at-microscopy.com} ; Thu, 10 Dec 2015 05:51:26 -0800 (PST) 21, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 21, 39 -- d=gmail.com; s=20120113; 21, 39 -- h=from:subject:reply-to:references:to:message-id:date:user-agent 21, 39 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 21, 39 -- bh=PcoXS2JV+2Z8N+Czw/vMEkqtfv+a5XnAiUABRzk3sTg=; 21, 39 -- b=CVBRleIJXX9F6GukrNc01HMniEtRewTxJypFx4ltqyIOJL16Im4x2OGToVv1Z9+7v+ 21, 39 -- 3ED/2nCTqm/N96J1xAiGTWC2NSM6G1uY9EaVb8tDv6gD8wWp/7l0zUZOG9Vhx5/L1ijZ 21, 39 -- hwhnoKsNTkhwYEjX46vvq9n9AhG1+7wbguwZ9rs2rC7evvY2IjFK1RyPMs9kH+cp7qsw 21, 39 -- QsxSLo/kATpQIkG3E+zGS7UAsuNlSiQSBLp+iMKC0mYW5j7ULzhbihkIx8+HXChA9eaV 21, 39 -- SotuSEeitYuP8O0C0QzSwjoSESWZsdjfcmRwKOSFctRNRZgf8/Wp/81AJmPzABgI1leZ 21, 39 -- QPDw== 21, 39 -- X-Received: by 10.55.31.214 with SMTP id n83mr15314664qkh.50.1449755486501; 21, 39 -- Thu, 10 Dec 2015 05:51:26 -0800 (PST) 21, 39 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1]) 21, 39 -- by smtp.googlemail.com with ESMTPSA id 4sm675609qkx.18.2015.12.10.05.51.25 21, 39 -- for {microscopy-at-microscopy.com} 21, 39 -- (version=TLSv1/SSLv3 cipher=OTHER); 21, 39 -- Thu, 10 Dec 2015 05:51:25 -0800 (PST) 21, 39 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 21, 39 -- X-Google-Original-From: MicroscopyListserver-NoReply 21, 39 -- {microscopylistserver-noreply-at-microscopy.com} 21, 39 -- Subject: viaWWW:Job position with FEI in Shangai 21, 39 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 39 -- References: {201512100416.tBA4GIoh011205-at-ns.microscopy.com} 21, 39 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 39 -- Message-ID: {5669835D.9070100-at-microscopy.com} 21, 39 -- Date: Thu, 10 Dec 2015 07:51:25 -0600 21, 39 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 21, 39 -- Gecko/20100101 Thunderbird/38.4.0 21, 39 -- MIME-Version: 1.0 21, 39 -- In-Reply-To: {201512100416.tBA4GIoh011205-at-ns.microscopy.com} 21, 39 -- Content-Type: text/plain; charset=utf-8; format=flowed 21, 39 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
MICROSCOPY FACILITY DIRECTOR - Neuroscience Research Institute
University of California, Santa Barbara
Under the direction of the Co-Director of the Neuroscience Research Institute and the Departmental Chair of MCDB, serves as a general resource on the use of microscopy toward achieving research goals of UCSB personnel. Maintains core microscopes and histology preparatory equipment and coordinates with the service representatives of the microscope companies to maintain core instruments at design specifications. In addition, assists in the preparation of grants by providing technical expertise and providing technical training for research personnel. In addition, serves an instructional role in training individuals and participating in classes involving microscopy and computational imaging.
Research Activity: Participation in research is encouraged if all of the above duties are fulfilled, after consultation with the advisory committee, the NRI Co-Directors and the MCDB Chair.
The University of California is an Equal Opportunity/Affirmative Action Employer, and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability status, protected veteran status, or any other characteristic protected by law. For primary consideration apply by 12/20/15, thereafter open until filled.
Apply online at https://jobs.ucsb.edu
Job #20150622
Must have a master's degree in a relevant field or equivalent combination of education and experience. Have demonstrated experience in microscopy and a strong interest in microscopy and its applications to ongoing research projects.
Note: Fingerprinting required.
==============================Original Headers============================== 12, 19 -- From judy.cushing-at-lifesci.ucsb.edu Thu Dec 10 10:45:09 2015 12, 19 -- Received: from mailsvr.lifesci.ucsb.edu (mailsvr.lifesci.ucsb.edu [128.111.90.44]) 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBAGj9sY005934 12, 19 -- for {microscopy-at-microscopy.com} ; Thu, 10 Dec 2015 10:45:09 -0600 12, 19 -- MIME-version: 1.0 12, 19 -- Content-transfer-encoding: 7BIT 12, 19 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 12, 19 -- Received: from [128.111.209.5] (nri-help.nri.ucsb.edu [128.111.209.5]) 12, 19 -- by mailsvr.lifesci.ucsb.edu 12, 19 -- (Oracle Communications Messaging Exchange Server 7u4-22.01 64bit (built Apr 21 12, 19 -- 2011)) with ESMTPSA id {0NZ50047KIJ0ZF00-at-mailsvr.lifesci.ucsb.edu} for 12, 19 -- microscopy-at-microscopy.com; Thu, 10 Dec 2015 08:45:01 -0800 (PST) 12, 19 -- Message-id: {5669AC0C.7090802-at-lifesci.ucsb.edu} 12, 19 -- Date: Thu, 10 Dec 2015 08:45:00 -0800 12, 19 -- From: Judy Cushing {judy.cushing-at-lifesci.ucsb.edu} 12, 19 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:17.0) Gecko/20130801 12, 19 -- Thunderbird/17.0.8 12, 19 -- To: microscopy-at-microscopy.com 12, 19 -- Subject: MICROSCOPY FACILITY DIRECTOR - Neuroscience Research Institute ==============================End of - Headers==============================
Hi Everyone, I would like to talk to someone who has a quant 3DFEG with gatan alto cryo system on it. I took off the anti contaminator in the microscope chamber a while back and now canât figure out how to get get it back in place. (my bad). I am hoping for an exchange of photos! Help!
Thanks
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu
==============================Original Headers============================== 7, 30 -- From gilpin-at-purdue.edu Thu Dec 10 10:58:07 2015 7, 30 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBAGw7M1010626 7, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Dec 2015 10:58:07 -0600 7, 30 -- Received: from exchange.purdue.edu (wpvexchub03.itap.purdue.edu [172.30.136.67]) 7, 30 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id tBAGvxi8029807 7, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Dec 2015 11:57:59 -0500 7, 30 -- Received: from WPVEXCMBX04.purdue.lcl ([169.254.4.141]) by 7, 30 -- WPVEXCHUB03.purdue.lcl ([172.30.136.67]) with mapi id 14.03.0224.002; Thu, 10 7, 30 -- Dec 2015 11:57:59 -0500 7, 30 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 7, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 30 -- Subject: quanta 3d FEG and Gatan Alto cryo system 7, 30 -- Thread-Topic: quanta 3d FEG and Gatan Alto cryo system 7, 30 -- Thread-Index: AQHRM2vrvJ/U+cDgK0Crl1RYrRoaRw== 7, 30 -- Date: Thu, 10 Dec 2015 16:57:58 +0000 7, 30 -- Message-ID: {D03FA8A0-862F-41C8-9261-91445EE2B86C-at-purdue.edu} 7, 30 -- Accept-Language: en-US 7, 30 -- Content-Language: en-US 7, 30 -- X-MS-Has-Attach: 7, 30 -- X-MS-TNEF-Correlator: 7, 30 -- x-originating-ip: [172.30.136.180] 7, 30 -- Content-Type: text/plain; charset="utf-8" 7, 30 -- Content-ID: {576B548F4B725640A9E96528C0EE1A0E-at-exchange.purdue.edu} 7, 30 -- MIME-Version: 1.0 7, 30 -- X-PMX-Version: 6.0.2.2308539 7, 30 -- X-PerlMx-URL-Scanned: Yes 7, 30 -- X-PerlMx-Virus-Scanned: Yes 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id tBAGw7M1010626 ==============================End of - Headers==============================
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Email: mlibbee-at-lbl.gov Name: Marissa Libbee
Organization: UCB/LBNL
Title-Subject: [Filtered] Joint faculty position UCB-LBNL in advanced materials characterization
Message: Sent on behalf of Dr. Andrew Minor
Dear MSA community,
I want to bring your attention to an open faculty position in the area of advanced materials characterization at UC Berkeley and LBNL. The appointment will be at the level of assistant professor, and will be a joint appointment between the Department of Materials Science and Engineering at UC Berkeley and LBNL. We are looking for outstanding candidates that demonstrate the potential to build a world-class research program in materials science that exploits and advances techniques for materials characterization enabled by the unique combination of facilities at UC Berkeley and LBNL, including the Molecular Foundry and the Advanced Light Source. We are specifically encouraging all qualified women and candidates from underrepresented groups to apply.
Applications are due on January 15, 2016, and candidates can view the full advertisement and apply directly at the following link: https://aprecruit.berkeley.edu/apply/JPF00862
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==============================Original Headers============================== 14, 41 -- From microscopy.listserver-at-gmail.com Sat Dec 12 06:30:56 2015 14, 41 -- Received: from mail-qg0-f44.google.com (mail-qg0-f44.google.com [209.85.192.44]) 14, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBCCUu8f026121 14, 41 -- for {microscopy-at-microscopy.com} ; Sat, 12 Dec 2015 06:30:56 -0600 14, 41 -- Received: by qgfb51 with SMTP id b51so13071813qgf.3 14, 41 -- for {microscopy-at-microscopy.com} ; Sat, 12 Dec 2015 04:30:49 -0800 (PST) 14, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 41 -- d=gmail.com; s=20120113; 14, 41 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 41 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 41 -- bh=BhqAmbRK18Mjq9qLrHcG6BBVNhJVlsptLzEyW4Yq8Ug=; 14, 41 -- b=HbwNjzlrsbeUEbb8NfVdmfdT336tdC8zGv9eZvYoF8EboKojuPJ7Hpb8GSnu/bIOn9 14, 41 -- Pzjg+n2sZaUzCVlVPE9AwiSvaiVD9js9AZkAU189lXg862d6kGcn+cCVXFVED2r9hUGo 14, 41 -- vbD/F9LzFC8GQ/0bpqRw0mpRq29N8A4ci+ZN9YR1IV9ijmNHAS5cAjDYqT7muUpskSVn 14, 41 -- f8DKvGnCANvE+9K87VwXpezrJpYjFLhe/YWdCnfHLn1DQhVsehSjNdmAb/V84lEngoei 14, 41 -- DYCSuXanS0youF9E2KzBZROIschzhpih7jfbO9ECvqVNG8bRnxnRnqQ9lFivhcouCvsx 14, 41 -- dZYA== 14, 41 -- X-Received: by 10.140.81.227 with SMTP id f90mr10238287qgd.54.1449923449017; 14, 41 -- Sat, 12 Dec 2015 04:30:49 -0800 (PST) 14, 41 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 41 -- by smtp.googlemail.com with ESMTPSA id v23sm3826624qkv.32.2015.12.12.04.30.47 14, 41 -- for {microscopy-at-microscopy.com} 14, 41 -- (version=TLSv1/SSLv3 cipher=OTHER); 14, 41 -- Sat, 12 Dec 2015 04:30:48 -0800 (PST) 14, 41 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 41 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 41 -- {microscopylistserver-noreply-at-microscopy.com} 14, 41 -- Subject: viaWWW:Joint faculty position UCB-LBNL in advanced materials 14, 41 -- characterization 14, 41 -- References: {201512111811.tBBIBH0j011021-at-ns.microscopy.com} 14, 41 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 41 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 41 -- X-Forwarded-Message-Id: {201512111811.tBBIBH0j011021-at-ns.microscopy.com} 14, 41 -- Message-ID: {566C1377.20300-at-microscopy.com} 14, 41 -- Date: Sat, 12 Dec 2015 06:30:47 -0600 14, 41 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 41 -- Gecko/20100101 Thunderbird/38.4.0 14, 41 -- MIME-Version: 1.0 14, 41 -- In-Reply-To: {201512111811.tBBIBH0j011021-at-ns.microscopy.com} 14, 41 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From conversion.seo-at-gmail.com Sat Dec 12 16:43:13 2015 Return-Path: {conversion.seo-at-gmail.com} Received: from gmail.com ([119.202.104.124]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBCMhAEZ030699 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 12 Dec 2015 16:43:12 -0600 Reply-To: conversion.seo-at-gmail.com
Every one who lives in this world of search engine optimization knows about the importance of having their website's link in High PR directories.
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A full-time, temporary position is available for a Research Assistant 1 or 2 at the Marine Biological Laboratory. This position is currently funded for 1 year with the possibility of continued funding. The successful candidate will work with a team of scientists investigating the role of pupils in the eyes of animals. A BA or MA degree (or equivalent) in biological sciences is required. Experience in functional morphology, microscopy (light and electron microscopy, confocal microscopy), immunocytochemistry and histochemical techniques are necessary for this position.
More details can be found at: https://mbl.simplehire.com/postings/3156
-- Louie Kerr | Director, Microscopy and Research Services | Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543 508-289-7273 | lkerr-at-mbl.edu | www.mbl.edu
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Dear All, I am looking for a large chamber FEG-SEM, like a Zeiss DSM 982 or a TESCAN MIRA, somewhere in Europe or even from abroad.
I am trying to evolve the quality of my nanoflight technique to "fly" around very small microstructures from a LaB6-driven SEM to the FEG-SEM. I would be very grateful if anything comes up in a financially achievable way... Maybe there is a FEG-SEM somewhere you want to get rid of this year? ;-)
That`s what I can do now with my Philips-FEI 525 SEM flying around a T-cell (genetically engineered to fight lymphoblastic cancer): https://vimeo.com/144734321 I am sure it would look unbelievable in the FEG-SEM in color ;-)
Best wishes, Stefan
Find out more about "nanoflight" in the showreel of my website: http://www.nanoflight.info/nanoflight2.html
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] Ion Mill/Polishing Systems
Message: Greetings,
My lab is looking to purchase an ion mill/polishing system. I am looking at bench top models such as the PIPS system from Gatan [or a similar device]. I need an estimate of the cost. I requested a quotes but so far no vendor has replied and I need to submit the request soon.
My request to the group: a rough estimate of a purchase cost for a bench top ion mill/polisher system such as Gatan PIPS II or similar device.
Thanks in advance.
Tom
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==============================Original Headers============================== 16, 40 -- From microscopy.listserver-at-gmail.com Mon Dec 14 17:04:00 2015 16, 40 -- Received: from mail-qg0-f44.google.com (mail-qg0-f44.google.com [209.85.192.44]) 16, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBEN3xxq003524 16, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2015 17:03:59 -0600 16, 40 -- Received: by qgx21 with SMTP id 21so19006618qgx.1 16, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2015 15:03:51 -0800 (PST) 16, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 40 -- d=gmail.com; s=20120113; 16, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 16, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 16, 40 -- bh=WrHe5zJalhZLIXcqK1JGZETrxZ07CCmkin6APGA21wY=; 16, 40 -- b=WDoyKgZcsCvhq22oUlXmKB5gcfkwhgIMehKAxNibhOgaJHnPoMvFOg4U+/7Lg22vnM 16, 40 -- 3ccUgPJSnMzpy6RMZ+fk8oqDhZQwefq8OoTv5Ka9fT6c57iTqZVbKTnxFu49MTle+EBw 16, 40 -- tMYgwYvyOg22Vtahu5bPdMLRfQ1qPx9YHWXQbjpa9JMbSIz4hBkagfwVluIUUQhrUAQJ 16, 40 -- yUZ2NrwYZ3thjfVFZj3dKj4jeiUfYKbmF+q+htNO6+VFJhHq4lfszTLvp4R9UALQ981c 16, 40 -- OdQazOIxnnllnwOJ//RAJrtVjZkpkrdREYAZHRPMGySPNJE1IlI2CsAGdouP1iaYh5Tk 16, 40 -- jWrA== 16, 40 -- X-Received: by 10.140.146.6 with SMTP id 6mr21384996qhs.100.1450134231472; 16, 40 -- Mon, 14 Dec 2015 15:03:51 -0800 (PST) 16, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 16, 40 -- by smtp.googlemail.com with ESMTPSA id w32sm6009673qgw.6.2015.12.14.15.03.50 16, 40 -- for {microscopy-at-microscopy.com} 16, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 16, 40 -- Mon, 14 Dec 2015 15:03:50 -0800 (PST) 16, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 16, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 40 -- {microscopylistserver-noreply-at-microscopy.com} 16, 40 -- Subject: viaWWW:Ion Mill/Polishing Systems 16, 40 -- References: {201512140500.tBE50Iwv012634-at-ns.microscopy.com} 16, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 40 -- X-Forwarded-Message-Id: {201512140500.tBE50Iwv012634-at-ns.microscopy.com} 16, 40 -- Message-ID: {566F4AD6.7050300-at-microscopy.com} 16, 40 -- Date: Mon, 14 Dec 2015 17:03:50 -0600 16, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 16, 40 -- Gecko/20100101 Thunderbird/38.4.0 16, 40 -- MIME-Version: 1.0 16, 40 -- In-Reply-To: {201512140500.tBE50Iwv012634-at-ns.microscopy.com} 16, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: chrisbrantner-at-email.gwu.edu Name: Chris Brantner
Organization: George Washington University Nanofabrication and Imaging Center
Title-Subject: [Filtered] recommendations for plasma cleaner
Message: Good afternoon microscopists,
I need to obtain a plasma cleaner for samples and holders for my new 200 kv TEM. I have never had one in my previous positions so I am looking for recommendations about how to choose one.
Thanks
Chris
Dr. Christine A. Brantner The George Washington University Senior Research Scientist, Electron Microscopy GW Nanofabrication and Imaging Center 800 22nd Street, NW Science and Engineering Hall B2825 Washington, DC 20052 202-994-3219 chrisbrantner-at-email.gwu.edu
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==============================Original Headers============================== 15, 40 -- From microscopy.listserver-at-gmail.com Mon Dec 14 17:05:01 2015 15, 40 -- Received: from mail-qg0-f46.google.com (mail-qg0-f46.google.com [209.85.192.46]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBEN4wD5003648 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2015 17:04:59 -0600 15, 40 -- Received: by qgx21 with SMTP id 21so19025557qgx.1 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Dec 2015 15:04:50 -0800 (PST) 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 40 -- d=gmail.com; s=20120113; 15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 40 -- bh=zoJ65WOQLjFosLMIhAsSQLIHAOUAYqeE2LALKXRboBo=; 15, 40 -- b=pse6eZ8qLfNMck8aCZh7MxbueHKFCX5tVU5BImSXZyqJCLxvvoRaM+qBx5A7Elt0gT 15, 40 -- JWLdo1mbcycVFs7SvOM64EVMeF9ZOdxinyqbn2LZconAyw9cRgy/+u6NNddnOoEzU15k 15, 40 -- 7RVi2QqSfgP9Wcyen71AwVbvk+qQ5xMRJDRU6Vu72TEc7b09OJ5NYE3cxiO/pys13s6/ 15, 40 -- NqYLFIRh8blGFSU796NXCco57Nv8nva4DgV6N7IapA3uqmDqMY63PUgCuM2pW09MfoIL 15, 40 -- kYqiie9nn6QIZ4zbJJNnxUSEXlGPB5VgXfOt+JikHIqtk7EH3ob/XQuYvOfpulDbyebc 15, 40 -- Nozg== 15, 40 -- X-Received: by 10.140.39.179 with SMTP id v48mr14884321qgv.98.1450134290496; 15, 40 -- Mon, 14 Dec 2015 15:04:50 -0800 (PST) 15, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 40 -- by smtp.googlemail.com with ESMTPSA id j64sm14681520qhc.36.2015.12.14.15.04.49 15, 40 -- for {microscopy-at-microscopy.com} 15, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 15, 40 -- Mon, 14 Dec 2015 15:04:50 -0800 (PST) 15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 40 -- {microscopylistserver-noreply-at-microscopy.com} 15, 40 -- Subject: viaWWW:recommendations for plasma cleaner 15, 40 -- References: {201512142023.tBEKNTXi020558-at-ns.microscopy.com} 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 40 -- X-Forwarded-Message-Id: {201512142023.tBEKNTXi020558-at-ns.microscopy.com} 15, 40 -- Message-ID: {566F4B11.4050309-at-microscopy.com} 15, 40 -- Date: Mon, 14 Dec 2015 17:04:49 -0600 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 40 -- Gecko/20100101 Thunderbird/38.4.0 15, 40 -- MIME-Version: 1.0 15, 40 -- In-Reply-To: {201512142023.tBEKNTXi020558-at-ns.microscopy.com} 15, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Greetings, We like the basic unit we got from Harrick. We do not use the gas mixer attachment that is available for the unit. No commerical interest, just a happy customer. The web page is here: harrickplasma.com/products/basic-plasma-cleaner
Good luck, Tobias
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-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ University of Mass. / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ Amherst, Massachusetts / /___ / \ \___/ \_____ USA 413-545-1533 www.bio.umass.edu/biology/baskin
==============================Original Headers============================== 5, 23 -- From baskin-at-bio.umass.edu Wed Dec 16 08:25:20 2015 5, 23 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBGEPI8G025123 5, 23 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2015 08:25:18 -0600 5, 23 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 5, 23 -- (authenticated bits=0) 5, 23 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id tBGEP8g3013818 5, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO); 5, 23 -- Wed, 16 Dec 2015 09:25:08 -0500 5, 23 -- Subject: Re: [Microscopy] viaWWW:recommendations for plasma cleaner 5, 23 -- To: microscnet {microscopy-at-microscopy.com} , chrisbrantner-at-email.gwu.edu 5, 23 -- References: {201512142323.tBENNDdw005772-at-ns.microscopy.com} 5, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 5, 23 -- Message-ID: {56717444.9010700-at-bio.umass.edu} 5, 23 -- Date: Wed, 16 Dec 2015 09:25:08 -0500 5, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 5, 23 -- Gecko/20100101 Thunderbird/38.4.0 5, 23 -- MIME-Version: 1.0 5, 23 -- In-Reply-To: {201512142323.tBENNDdw005772-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Wed, 16 Dec 2015 09:25:08 -0500 (EST) 5, 23 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] Thanks and Happy Holidays
Message: Greetings,
Wanted to take a moment to thank everyone who helped with my request for info on ion mill/polisher systems. The response was overwhelming and very helpful. Saved me some hair pulling and teeth gnashing.
Thanks again & Happy Holidays all around.
Tom
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==============================Original Headers============================== 15, 40 -- From microscopy.listserver-at-gmail.com Wed Dec 16 17:34:35 2015 15, 40 -- Received: from mail-ig0-f171.google.com (mail-ig0-f171.google.com [209.85.213.171]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBGNYYDo022748 15, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2015 17:34:34 -0600 15, 40 -- Received: by mail-ig0-f171.google.com with SMTP id to18so71781697igc.0 15, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2015 15:34:26 -0800 (PST) 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 40 -- d=gmail.com; s=20120113; 15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 40 -- bh=bqp/rBjuSaB6krUMrZ7bNt11lOUxP/BBEstRtYKktEs=; 15, 40 -- b=wv4c7jLctIUSLX7WVAmu21X/ZYK+sb29QdrVMbbPgFra15HQ2nW6ny29UyoThfXyoS 15, 40 -- b9UhtqRGswptEnhfRI66t1XH0EFbBIHPyQmXIzhug5zx2kPWvqkwU4Ps1NwAT289ve2m 15, 40 -- MRlLI+BliLjpAsCeuv1865AgzGPVN6rQKYp8exBKPzmWyvRaqygPg/Ge2kN2RYqtcwCD 15, 40 -- dEhGytbh2R5/HdSoIrOcvSUG5hXzRooe8mP57tvZf0vFZvUWgV8G8YKLufz9NQdzKpKn 15, 40 -- YOiigda44/ljSSzs4zEMS6fHtPYAbOLPOPdl6kTcv1g2Q8UM6MaoWmp8wwwus5t2HOdM 15, 40 -- u+Og== 15, 40 -- X-Received: by 10.50.47.40 with SMTP id a8mr434770ign.49.1450308866653; 15, 40 -- Wed, 16 Dec 2015 15:34:26 -0800 (PST) 15, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 40 -- by smtp.googlemail.com with ESMTPSA id eg1sm3641095igb.18.2015.12.16.15.34.25 15, 40 -- for {microscopy-at-microscopy.com} 15, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 15, 40 -- Wed, 16 Dec 2015 15:34:26 -0800 (PST) 15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 40 -- {microscopylistserver-noreply-at-microscopy.com} 15, 40 -- Subject: viaWWW:Thanks for help on ion mill/polisher 15, 40 -- References: {201512161724.tBGHOcRN004554-at-ns.microscopy.com} 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 40 -- X-Forwarded-Message-Id: {201512161724.tBGHOcRN004554-at-ns.microscopy.com} 15, 40 -- Message-ID: {5671F501.1050508-at-microscopy.com} 15, 40 -- Date: Wed, 16 Dec 2015 17:34:25 -0600 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 40 -- Gecko/20100101 Thunderbird/38.4.0 15, 40 -- MIME-Version: 1.0 15, 40 -- In-Reply-To: {201512161724.tBGHOcRN004554-at-ns.microscopy.com} 15, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Colleagues: We are in the process of upgrading our TEM camera system and are exploring several of the side mount camera systems that are currently available. Our current TEM has an AMT XR611 and has worked well over the years. We have been considering a camera upgrade for the scope and are looking at severall models, including the AMT BioSprint in a mid-mount position. One option that was presented to me was a Morada G3 TEM camera, but I am not very familiar with that company.
Any insight or information about the Morada Camera would be greatly appreciated!
Best Regards Kevin
Kevin J. McCarthy, Ph.D. Professor, Department of Pathology LSU Health Sciences Center-Shreveport
==============================Original Headers============================== 4, 34 -- From KMccar2-at-lsuhsc.edu Wed Dec 16 18:52:59 2015 4, 34 -- Received: from exchipmx01.lsuhsc.edu (exchipmx01.lsuhsc.edu [155.58.210.27]) 4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBH0qrlX028089 4, 34 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2015 18:52:55 -0600 4, 34 -- X-IronPort-Anti-Spam-Filtered: true 4, 34 -- X-IronPort-Anti-Spam-Result: A2DjBQAFB3JW/7/wFKxexFaHdRABAQEBAQEBfwtBDoNsOlEBPkImAQSIQgWcY54KAYNuASCGWIINhzmDW4EaBZZ8AY1HnRs5K4FWOx2BVj6BdIFlgUkBAQE 4, 34 -- X-IPAS-Result: A2DjBQAFB3JW/7/wFKxexFaHdRABAQEBAQEBfwtBDoNsOlEBPkImAQSIQgWcY54KAYNuASCGWIINhzmDW4EaBZZ8AY1HnRs5K4FWOx2BVj6BdIFlgUkBAQE 4, 34 -- Received: from exchmr01.lsuhsc.edu ([172.20.240.191]) 4, 34 -- by exchipmx01.lsuhsc.edu with ESMTP/TLS/AES256-SHA; 16 Dec 2015 18:52:45 -0600 4, 34 -- Received: from exchmr01.lsuhsc.edu (172.20.240.191) by exchmr01.lsuhsc.edu 4, 34 -- (172.20.240.191) with Microsoft SMTP Server (TLS) id 15.0.1130.7; Wed, 16 Dec 4, 34 -- 2015 18:52:45 -0600 4, 34 -- Received: from SH-EXCHHUB3.master.lsuhsc.edu (155.58.112.81) by 4, 34 -- exchmr01.lsuhsc.edu (172.20.240.191) with Microsoft SMTP Server (TLS) id 4, 34 -- 15.0.1130.7 via Frontend Transport; Wed, 16 Dec 2015 18:52:45 -0600 4, 34 -- Received: from SH-EXCHMB2.master.lsuhsc.edu ([169.254.2.248]) by 4, 34 -- SH-ExchHub3.master.lsuhsc.edu ([155.58.112.81]) with mapi id 14.03.0248.002; 4, 34 -- Wed, 16 Dec 2015 18:51:41 -0600 4, 34 -- From: "McCarthy, Kevin" {KMccar2-at-lsuhsc.edu} 4, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 34 -- Subject: TEM camera recommendations 4, 34 -- Thread-Topic: TEM camera recommendations 4, 34 -- Thread-Index: AdE4ZR5dgrF3wxjxTe2Zcfsj82cNwA== 4, 34 -- Date: Thu, 17 Dec 2015 00:51:52 +0000 4, 34 -- Message-ID: {A71B140A-9A2B-482F-A499-6FC7E2BC0F4C-at-lsuhsc.edu} 4, 34 -- Accept-Language: en-US 4, 34 -- Content-Language: en-US 4, 34 -- X-MS-Has-Attach: 4, 34 -- X-MS-TNEF-Correlator: 4, 34 -- Content-Type: text/plain; charset="us-ascii" 4, 34 -- Content-ID: {298B30F41210D34088D5398CF3A2B4FB-at-lsuhsc.edu} 4, 34 -- MIME-Version: 1.0 4, 34 -- Content-Transfer-Encoding: 8bit 4, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tBH0qrlX028089 ==============================End of - Headers==============================
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Dear Listers,
Does anyone know what the percentage concentration of Lead Citrate is in Reynolds Lead Citrate after the conversion of the two components?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Thu Dec 17 14:34:46 2015 7, 37 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBHKYkEH016302 7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 14:34:46 -0600 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id BCA7B381D4E 7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 14:19:28 -0600 (CST) 7, 37 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id E8C22381D82 7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 14:19:27 -0600 (CST) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 37 -- 14.03.0235.001; Thu, 17 Dec 2015 14:34:34 -0600 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Percentage concentration of Reynold's Lead Citrate 7, 37 -- Thread-Topic: Percentage concentration of Reynold's Lead Citrate 7, 37 -- Thread-Index: AdE5Chm2pNRfVx4eTCaDimuv97y4Ng== 7, 37 -- Date: Thu, 17 Dec 2015 20:34:33 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AC8AB282-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: 762f530f-529f-429f-9b34-a10cb4826c9d 7, 37 -- X-VPM-HOST: zixvpm02.unmc.edu 7, 37 -- X-VPM-GROUP-ID: e9c4fb7d-2f19-4dbc-80de-cf4f4e4205bc 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tBHKYkEH016302 ==============================End of - Headers==============================
I would like to hear privately from anyone using Hummingbird Scientific's flow cell e-chips on their TEM.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 8, 39 -- From tbargar-at-unmc.edu Thu Dec 17 14:37:16 2015 8, 39 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 8, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBHKbFkc016466 8, 39 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 14:37:15 -0600 8, 39 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 8, 39 -- by Outbound.unmc.edu (Proprietary) with SMTP id A2E93381E52 8, 39 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 14:21:58 -0600 (CST) 8, 39 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 8, 39 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 8, 39 -- (No client certificate requested) 8, 39 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id 8FE07381E05 8, 39 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 14:21:57 -0600 (CST) 8, 39 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 8, 39 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 8, 39 -- 14.03.0235.001; Thu, 17 Dec 2015 14:36:51 -0600 8, 39 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 8, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 39 -- Subject: I want to hear from users of Hummingbird Scientific TEM flow cell 8, 39 -- e-chips. 8, 39 -- Thread-Topic: I want to hear from users of Hummingbird Scientific TEM flow 8, 39 -- cell e-chips. 8, 39 -- Thread-Index: AdE5CnqIUy5Fp22iSWWFog94u/GSFA== 8, 39 -- Date: Thu, 17 Dec 2015 20:36:51 +0000 8, 39 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AC8AB28F-at-UNMCEX2.unmcresforest.org} 8, 39 -- Accept-Language: en-US 8, 39 -- Content-Language: en-US 8, 39 -- X-MS-Has-Attach: 8, 39 -- X-MS-TNEF-Correlator: 8, 39 -- x-originating-ip: [10.8.64.15] 8, 39 -- Content-Type: text/plain; charset="us-ascii" 8, 39 -- MIME-Version: 1.0 8, 39 -- X-VPM-MSG-ID: b2a5a8f5-c33a-47a8-bc1f-e67bcde3f52c 8, 39 -- X-VPM-HOST: zixvpm02.unmc.edu 8, 39 -- X-VPM-GROUP-ID: 6697104b-2371-4b55-8583-277ec982071b 8, 39 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 8, 39 -- X-VPM-CERT-FLAG: 0 8, 39 -- X-VPM-IS-HYBRID: 0 8, 39 -- Content-Transfer-Encoding: 8bit 8, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tBHKbFkc016466 ==============================End of - Headers==============================
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Email: johnshields59-at-gmail.com Name: John P Shields
Organization: University of Georgia
Title-Subject: [Filtered] Folded Paper microscope New Yorker
Message: An interesting article in the Dec. 2015 New Yorker about the Folded paper microscope. Apologies if this is a repeat. http://www.newyorker.com/magazine/2015/12/21/through-the-looking-glass-annals-of-science-carolyn-kormann
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Email: mikroskop-at-gmail.com Name: Jack
Title-Subject: [Filtered] linear measurement significant figures
Message: Hello Microscopists- I have a very fundamental micrometry question:
What is the valid number of significant figures that can be reported for linear measurements made with a light microscope coupled with a digital camera?
This is an incredibly basic question, I feel a bit naĂŻve/ sheepish for asking it but I have not been able to find suitable references that address this issue. Here are the experimental parameters/ conditions under which I am making measurements:
Nikon PLM with a 20x, 0.45 NA Plan Apo objective The theoretical resolution of the objective is: (0.61*lambda)/NA: (.61*0.550 um)/0.45= 0.746 um
The CCD camera attached to the microscope has a chip size that measures 2560 x 1920 pixels; each pixel is 3.4 um (according to manufacturerÂs specs).
Using a Edmund Optics Certified (NIST-traceable) stage micrometer (typical 10 um divisions)
Calibration: Draw line that starts at 0 and ends at 400 um. This equates to 2350 pixels, thus the calibration is 0.1702 um/px or 5.8750 px/um.
Linear measurements and other shape parameters are calculated using typical image analysis software return values with a huge number of decimal places (7+)... these all can't be valid!
Thank you very much for any information/ reference you can provide.
-Jack
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==============================Original Headers============================== 29, 40 -- From microscopy.listserver-at-gmail.com Thu Dec 17 16:10:28 2015 29, 40 -- Received: from mail-ig0-f173.google.com (mail-ig0-f173.google.com [209.85.213.173]) 29, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBHMAOsN026373 29, 40 -- for {microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 16:10:25 -0600 29, 40 -- Received: by mail-ig0-f173.google.com with SMTP id to18so21788683igc.0 29, 40 -- for {microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 14:10:17 -0800 (PST) 29, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 29, 40 -- d=gmail.com; s=20120113; 29, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 29, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 29, 40 -- bh=Fqxcp28tIa49hTaY8XQL0XXEdibnZcaKBbxwvkap+sc=; 29, 40 -- b=QPRdClYAlKBRueD+VXZKy0xin2eXuGh9YlYp6KxEmLAv3wWsvt4WrjrUgfPIWWjZ+f 29, 40 -- zaJONWM3PnMuhyLGKEHGXgotwCvJJGHqF+qAI7OIqHwMtB9U65owoRXdQl3ij/gqD7PC 29, 40 -- zFrKjXCzhFl+bldD0v3DmYU6vYmk8vCTNL2bl5gMCiAVI4oveq+AvCxyekPblSnZ2oYL 29, 40 -- 0jn8Dy2IHFNmm1KgE/5WoXyXmKnh9XQnC/zoBcUKDD1EQh9wRCp4V/UQFLqcQLmzvXNQ 29, 40 -- sptbuDFD6Ch12zH1/7IgBcC9wS0QgmKODGByA/2jEzcIWPONfOtC+C7QyMldHWT5q1vy 29, 40 -- PevA== 29, 40 -- X-Received: by 10.50.79.202 with SMTP id l10mr6416087igx.46.1450390216878; 29, 40 -- Thu, 17 Dec 2015 14:10:16 -0800 (PST) 29, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 29, 40 -- by smtp.googlemail.com with ESMTPSA id 7sm1781033igr.0.2015.12.17.14.10.16 29, 40 -- for {microscopy-at-microscopy.com} 29, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 29, 40 -- Thu, 17 Dec 2015 14:10:16 -0800 (PST) 29, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 29, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 29, 40 -- {microscopylistserver-noreply-at-microscopy.com} 29, 40 -- Subject: viaWWW:LM: linear measurement significant figures 29, 40 -- References: {201512171922.tBHJMVFM014941-at-ns.microscopy.com} 29, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 29, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 29, 40 -- X-Forwarded-Message-Id: {201512171922.tBHJMVFM014941-at-ns.microscopy.com} 29, 40 -- Message-ID: {567332C7.2090800-at-microscopy.com} 29, 40 -- Date: Thu, 17 Dec 2015 16:10:15 -0600 29, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 29, 40 -- Gecko/20100101 Thunderbird/38.4.0 29, 40 -- MIME-Version: 1.0 29, 40 -- In-Reply-To: {201512171922.tBHJMVFM014941-at-ns.microscopy.com} 29, 40 -- Content-Type: text/plain; charset=UTF-8; format=flowed 29, 40 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Dear Friends I would like to ask this wise List to help me identifying an object found in an animal tissue biopsy -please click http://www.eikonika.net/v2/downloads/stranger.jpg The object is apparently biological, sized 20u and resembles to small pollen. The way it is mixed with blood an mucus makes difficult to imagine that this object came as a contaminant after fixation. Thanks for your time! yorgos
It's a diatom. Lot similar to this, and many others, on our website:
http://www.mta.ca/dmf
This could have entered your specimen processing stream in a variety of ways - water, some filter device, random dust, even airborne. They're really everywhere.
Jim
On 18/12/2015 12:16 PM, eikonika-at-otenet.gr wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Friends } I would like to ask this wise List to help me identifying an object found in } an animal tissue biopsy -please click } http://www.eikonika.net/v2/downloads/stranger.jpg The object is apparently } biological, sized 20u and resembles to small pollen. The way it is mixed } with blood an mucus makes difficult to imagine that this object came as a } contaminant after fixation. } Thanks for your time! } yorgos } } } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.net www.aim.cat } ************************************* } } } } } ==============================Original Headers============================== } 7, 19 -- From eikonika-at-otenet.gr Fri Dec 18 10:05:25 2015 } 7, 19 -- Received: from sphinx.otenet.gr (smtp-out34.otenet.gr [83.235.69.34]) } 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBIG5NlW010788 } 7, 19 -- for {microscopy-at-microscopy.com} ; Fri, 18 Dec 2015 10:05:23 -0600 } 7, 19 -- Received: from ozymandias (ppp-2-84-174-131.home.otenet.gr [2.84.174.131]) } 7, 19 -- by sphinx.otenet.gr (ESMTP) with ESMTP } 7, 19 -- for {microscopy-at-microscopy.com} ; Fri, 18 Dec 2015 18:05:13 +0200 (EET) } 7, 19 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} } 7, 19 -- To: {microscopy-at-microscopy.com} } 7, 19 -- Subject: unexpected object in SEM } 7, 19 -- Date: Fri, 18 Dec 2015 18:05:10 +0200 } 7, 19 -- Message-ID: {000801d139ad$e07aba80$a1702f80$-at-otenet.gr} } 7, 19 -- MIME-Version: 1.0 } 7, 19 -- Content-Type: text/plain; } 7, 19 -- charset="us-ascii" } 7, 19 -- Content-Transfer-Encoding: 7bit } 7, 19 -- X-Mailer: Microsoft Outlook 14.0 } 7, 19 -- Thread-Index: AdE5rdsqD/C/7usXSoSHUBKfHXvAmA== } 7, 19 -- Content-Language: en-us } ==============================End of - Headers==============================
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James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
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==============================Original Headers============================== 12, 21 -- From jehrman-at-mta.ca Fri Dec 18 11:26:49 2015 12, 21 -- Received: from smtpy.mta.ca (smtpy.mta.ca [138.73.1.203]) 12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBIHQmEh016897 12, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Dec 2015 11:26:48 -0600 12, 21 -- Received: from [138.73.22.169] (port=61914) 12, 21 -- by smtpy.mta.ca with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 12, 21 -- (Exim 4.84) 12, 21 -- (envelope-from {jehrman-at-mta.ca} ) 12, 21 -- id 1a9yne-0004SL-FJ; Fri, 18 Dec 2015 13:26:38 -0400 12, 21 -- Subject: Re: [Microscopy] unexpected object in SEM 12, 21 -- To: eikonika-at-otenet.gr, Microscopy Listserv {Microscopy-at-microscopy.com} 12, 21 -- References: {201512181616.tBIGGjH5011588-at-ns.microscopy.com} 12, 21 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 12, 21 -- Message-ID: {567441CE.1090102-at-mta.ca} 12, 21 -- Date: Fri, 18 Dec 2015 13:26:38 -0400 12, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 12, 21 -- Thunderbird/38.4.0 12, 21 -- MIME-Version: 1.0 12, 21 -- In-Reply-To: {201512181616.tBIGGjH5011588-at-ns.microscopy.com} 12, 21 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You could spend some time, gather more morphological data and size and ID if desired.
Tony
......................................... Andrew Anthony "Tony" Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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-----Original Message----- X-from: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr] Sent: Friday, December 18, 2015 12:41 PM To: ph2-at-sprynet.com
Dear Friends I would like to ask this wise List to help me identifying an object found in an animal tissue biopsy -please click http://www.eikonika.net/v2/downloads/stranger.jpg The object is apparently biological, sized 20u and resembles to small pollen. The way it is mixed with blood an mucus makes difficult to imagine that this object came as a contaminant after fixation. Thanks for your time! yorgos
Dr. Nikas, This looks like either a diatome or dinoflagellate. What is the tissue (I see RBC's and microvilli or cilia). Was this animal underwater? Let us know when you find out.
Sincerely, Michael Delannoy Johns Hopkins Microscope Facility
-----Original Message----- X-from: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr] Sent: Friday, December 18, 2015 1:09 PM To: delannoy-at-jhmi.edu
Dear Friends I would like to ask this wise List to help me identifying an object found in an animal tissue biopsy -please click http://www.eikonika.net/v2/downloads/stranger.jpg The object is apparently biological, sized 20u and resembles to small pollen. The way it is mixed with blood an mucus makes difficult to imagine that this object came as a contaminant after fixation. Thanks for your time! yorgos
Thank you for your replies, all agreeing that the stranger is a small diatom present in the water. So I can imagine how this was found mixed with blood and mucus in my specimen (it is an endometrial biopsy from a lady's womb with endometritis -ciliated and secretory cells and RBC are in the scene): Small diatome-} penetrates water filters -} arrives into my fixative-} binds to the tissue upon fixation-} visible on the sample. Best regards yorgos
Dear Friends I would like to ask this wise List to help me identifying an object found in an animal tissue biopsy -please click http://www.eikonika.net/v2/downloads/stranger.jpg The object is apparently biological, sized 20u and resembles to small pollen. The way it is mixed with blood an mucus makes difficult to imagine that this object came as a contaminant after fixation. Thanks for your time! yorgos
Of course, most of the digits generated are going to be nonsense. It is good that you asked .
Certainly you should not quote values beyond the resolution of your system. That will be a convolution of your microscope resolution and your camera resolution. For most current cameras, I would suppose that the microscope is the largest portion. That seems to be your case given your figures.
There is also the bigger, practical question of operator reproducibility. How consistently can you measure your 400-um micrometer? If you get a standard deviation of 4 um on your micrometer, then you should limit yourself to three digits. Repeat the experiment at various magnifications and see what you get. I expect you will find the operator is the limiting factor.
Warren Straszheim
-----Original Message----- Sent: Thursday, December 17, 2015 4:33 PM
X-from: mikroskop-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mikroskop-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mikroskop-at-gmail.com Name: Jack
Title-Subject: [Filtered] linear measurement significant figures
Message: Hello Microscopists- I have a very fundamental micrometry question:
What is the valid number of significant figures that can be reported for linear measurements made with a light microscope coupled with a digital camera?
This is an incredibly basic question, I feel a bit naive/ sheepish for asking it but I have not been able to find suitable references that address this issue. Here are the experimental parameters/ conditions under which I am making measurements:
Nikon PLM with a 20x, 0.45 NA Plan Apo objective The theoretical resolution of the objective is: (0.61*lambda)/NA: (.61*0.550 um)/0.45= 0.746 um
The CCD camera attached to the microscope has a chip size that measures 2560 x 1920 pixels; each pixel is 3.4 um (according to manufacturerÂ's specs).
Using a Edmund Optics Certified (NIST-traceable) stage micrometer (typical 10 um divisions)
Calibration: Draw line that starts at 0 and ends at 400 um. This equates to 2350 pixels, thus the calibration is 0.1702 um/px or 5.8750 px/um.
Linear measurements and other shape parameters are calculated using typical image analysis software return values with a huge number of decimal places (7+)... these all can't be valid!
Thank you very much for any information/ reference you can provide.
-Jack
Login Host: 63.167.71.254 Listserver Email Form V - 20120416 =========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
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==============================Original Headers============================== 29, 40 -- From microscopy.listserver-at-gmail.com Thu Dec 17 16:10:28 2015 29, 40 -- Received: from mail-ig0-f173.google.com (mail-ig0-f173.google.com [209.85.213.173]) 29, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBHMAOsN026373 29, 40 -- for {microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 16:10:25 -0600 29, 40 -- Received: by mail-ig0-f173.google.com with SMTP id to18so21788683igc.0 29, 40 -- for {microscopy-at-microscopy.com} ; Thu, 17 Dec 2015 14:10:17 -0800 (PST) 29, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 29, 40 -- d=gmail.com; s=20120113; 29, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 29, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 29, 40 -- bh=Fqxcp28tIa49hTaY8XQL0XXEdibnZcaKBbxwvkap+sc=; 29, 40 -- b=QPRdClYAlKBRueD+VXZKy0xin2eXuGh9YlYp6KxEmLAv3wWsvt4WrjrUgfPIWWjZ+f 29, 40 -- zaJONWM3PnMuhyLGKEHGXgotwCvJJGHqF+qAI7OIqHwMtB9U65owoRXdQl3ij/gqD7PC 29, 40 -- zFrKjXCzhFl+bldD0v3DmYU6vYmk8vCTNL2bl5gMCiAVI4oveq+AvCxyekPblSnZ2oYL 29, 40 -- 0jn8Dy2IHFNmm1KgE/5WoXyXmKnh9XQnC/zoBcUKDD1EQh9wRCp4V/UQFLqcQLmzvXNQ 29, 40 -- sptbuDFD6Ch12zH1/7IgBcC9wS0QgmKODGByA/2jEzcIWPONfOtC+C7QyMldHWT5q1vy 29, 40 -- PevA== 29, 40 -- X-Received: by 10.50.79.202 with SMTP id l10mr6416087igx.46.1450390216878; 29, 40 -- Thu, 17 Dec 2015 14:10:16 -0800 (PST) 29, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 29, 40 -- by smtp.googlemail.com with ESMTPSA id 7sm1781033igr.0.2015.12.17.14.10.16 29, 40 -- for {microscopy-at-microscopy.com} 29, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 29, 40 -- Thu, 17 Dec 2015 14:10:16 -0800 (PST) 29, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 29, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 29, 40 -- {microscopylistserver-noreply-at-microscopy.com} 29, 40 -- Subject: viaWWW:LM: linear measurement significant figures 29, 40 -- References: {201512171922.tBHJMVFM014941-at-ns.microscopy.com} 29, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 29, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 29, 40 -- X-Forwarded-Message-Id: {201512171922.tBHJMVFM014941-at-ns.microscopy.com} 29, 40 -- Message-ID: {567332C7.2090800-at-microscopy.com} 29, 40 -- Date: Thu, 17 Dec 2015 16:10:15 -0600 29, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 29, 40 -- Gecko/20100101 Thunderbird/38.4.0 29, 40 -- MIME-Version: 1.0 29, 40 -- In-Reply-To: {201512171922.tBHJMVFM014941-at-ns.microscopy.com} 29, 40 -- Content-Type: text/plain; charset=UTF-8; format=flowed 29, 40 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 27, 48 -- From wesaia-at-iastate.edu Fri Dec 18 22:09:59 2015 27, 48 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2on0074.outbound.protection.outlook.com [65.55.169.74]) 27, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBJ49rWQ026372 27, 48 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Dec 2015 22:09:53 -0600 27, 48 -- Received: from BN1PR0401MB0977.namprd04.prod.outlook.com (10.160.79.140) by 27, 48 -- BN1PR0401MB0978.namprd04.prod.outlook.com (10.160.79.141) with Microsoft SMTP 27, 48 -- Server (TLS) id 15.1.361.13; Sat, 19 Dec 2015 04:09:43 +0000 27, 48 -- Received: from BN1PR0401MB0977.namprd04.prod.outlook.com ([10.160.79.140]) by 27, 48 -- BN1PR0401MB0977.namprd04.prod.outlook.com ([10.160.79.140]) with mapi id 27, 48 -- 15.01.0355.012; Sat, 19 Dec 2015 04:09:43 +0000 27, 48 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 27, 48 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 27, 48 -- CC: "mikroskop-at-gmail.com" {mikroskop-at-gmail.com} 27, 48 -- Subject: RE: [Microscopy] viaWWW:LM: linear measurement significant figures 27, 48 -- Thread-Topic: [Microscopy] viaWWW:LM: linear measurement significant figures 27, 48 -- Thread-Index: AQHRORr2Q4pE/o4SbEqUnZvjBvj8aJ7RszhA 27, 48 -- Date: Sat, 19 Dec 2015 04:09:43 +0000 27, 48 -- Message-ID: {BN1PR0401MB0977F7E252E55C9E5D7901C6D7E20-at-BN1PR0401MB0977.namprd04.prod.outlook.com} 27, 48 -- References: {201512172233.tBHMXEOx028956-at-ns.microscopy.com} 27, 48 -- In-Reply-To: {201512172233.tBHMXEOx028956-at-ns.microscopy.com} 27, 48 -- Accept-Language: en-US 27, 48 -- Content-Language: en-US 27, 48 -- X-MS-Has-Attach: 27, 48 -- X-MS-TNEF-Correlator: 27, 48 -- authentication-results: spf=none (sender IP is ) 27, 48 -- smtp.mailfrom=wesaia-at-iastate.edu; 27, 48 -- x-originating-ip: [69.66.102.132] 27, 48 -- x-microsoft-exchange-diagnostics: 1;BN1PR0401MB0978;5:SCQEyxYvp6EU7xJA6m8ICWLPOqZuGsZkfKcM5pQWBSH8Pmu1RQ/AfyPnkP02etKtqLqEAFszvYnDILkqiRVg4wyVEzuEkX5VcMZVHg4+44EOg369AfFpf0bp+E2WSVbSpMCtsIy7EZij4pmE5jCilw==;24:W/KAMtNYKHl8O7zheLVTwvsB+SHFj4CZEoFCHEPaOWOrhXbsV/3jfB65+FlntNLuX24B7bg78al0somltoIsMFaO65sDsr7Q5d0a56LuK8Y= 27, 48 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(42139001);SRVR:BN1PR0401MB0978; 27, 48 -- x-microsoft-antispam-prvs: {BN1PR0401MB0978DEF36743B0A17F15021FD7E20-at-BN1PR0401MB0978.namprd04.prod.outlook.com} 27, 48 -- x-exchange-antispam-report-test: UriScan:(8415204561270); 27, 48 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(520078)(5005006)(8121501046)(3002001)(10201501046);SRVR:BN1PR0401MB0978;BCL:0;PCL:0;RULEID:;SRVR:BN1PR0401MB0978; 27, 48 -- x-forefront-prvs: 07954CC105 27, 48 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(377454003)(252514010)(45984002)(13464003)(199003)(189002)(86362001)(5003600100002)(19580395003)(5002640100001)(50986999)(105586002)(97736004)(81156007)(575784001)(33656002)(122556002)(40100003)(66066001)(75432002)(6116002)(90282001)(106356001)(3846002)(106116001)(99286002)(76176999)(325944007)(102836003)(74316001)(1096002)(92566002)(1220700001)(11100500001)(87936001)(5008740100001)(101416001)(5890100001)(88552001)(2950100001)(64544003)(5004730100002)(2501003)(89122001)(586003)(15975445007)(54356999)(2351001)(110136002)(76576001)(189998001)(5001960100002)(77096005)(2900100001)(19580405001)(10400500002)(1720100001);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR0401MB0978;H:BN1PR0401MB0977.namprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 27, 48 -- received-spf: None (protection.outlook.com: iastate.edu does not designate 27, 48 -- permitted sender hosts) 27, 48 -- spamdiagnosticoutput: 1:23 27, 48 -- spamdiagnosticmetadata: NSPM 27, 48 -- Content-Type: text/plain; charset="iso-8859-1" 27, 48 -- MIME-Version: 1.0 27, 48 -- X-OriginatorOrg: iastate.edu 27, 48 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 19 Dec 2015 04:09:43.3122 27, 48 -- (UTC) 27, 48 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 27, 48 -- X-MS-Exchange-CrossTenant-id: 0347d89a-0174-4dd3-adeb-3339c89c35f5 27, 48 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN1PR0401MB0978 27, 48 -- Content-Transfer-Encoding: 8bit 27, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tBJ49rWQ026372 ==============================End of - Headers==============================
If you have any filters in your water lines for the water you or the sample provider use to make solutions, check them for diatomaceous earth filters. Fairly common, and another source of diatoms as contaminants.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
________________________________________ X-from: eikonika-at-otenet.gr [eikonika-at-otenet.gr] Sent: Friday, December 18, 2015 11:01 PM To: Oshel, Philip Eugene
Thank you for your replies, all agreeing that the stranger is a small diatom present in the water. So I can imagine how this was found mixed with blood and mucus in my specimen (it is an endometrial biopsy from a lady's womb with endometritis -ciliated and secretory cells and RBC are in the scene): Small diatome-} penetrates water filters -} arrives into my fixative-} binds to the tissue upon fixation-} visible on the sample. Best regards yorgos
Dear Friends I would like to ask this wise List to help me identifying an object found in an animal tissue biopsy -please click http://www.eikonika.net/v2/downloads/stranger.jpg The object is apparently biological, sized 20u and resembles to small pollen. The way it is mixed with blood an mucus makes difficult to imagine that this object came as a contaminant after fixation. Thanks for your time! yorgos
RE: viaWWW:LM: linear measurement significant figures
I think I may have set a new personal record - 60 out-of-office automatic replies. I suppose I should have expected that as the holidays approach. I might have done even better by waiting until next week.
16 responses came from vendors, some of who I actually deal with. The others let me know anyway. ;) 31 responses came from various labs. I have dealings with none of them, although I recognized many respected institutions. 13 more came from organizations I could not recognize as vendors or labs. Of course, that means I have dealings with none of them.
The list FAQs state "Do not set your Email program to deliver "out of the office/vacation" messages. If you will be gone, you should unsubscribe from the list and subscribe again when you return." Please consider it, especially vendors. I know you would like to keep your name in front of your potential customers. However, it seems counterproductive when it is by way of one of these messages. Besides, it seems that some of you are always out of the office and on the road.
Regardless, I thank the posters from the past year. I appreciate you sharing of your time and wisdom.
And thank you, Nestor, for keeping this forum going.
Merry Christmas and a Happy New Year.
Warren
==============================Original Headers============================== 9, 45 -- From wesaia-at-iastate.edu Sun Dec 20 22:11:02 2015 9, 45 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1bon0075.outbound.protection.outlook.com [157.56.111.75]) 9, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBL4Auik002496 9, 45 -- for {Microscopy-at-microscopy.com} ; Sun, 20 Dec 2015 22:10:59 -0600 9, 45 -- Received: from BN1PR0401MB0977.namprd04.prod.outlook.com (10.160.79.140) by 9, 45 -- BN1PR0401MB0980.namprd04.prod.outlook.com (10.160.79.143) with Microsoft SMTP 9, 45 -- Server (TLS) id 15.1.361.13; Mon, 21 Dec 2015 03:51:32 +0000 9, 45 -- Received: from BN1PR0401MB0977.namprd04.prod.outlook.com ([10.160.79.140]) by 9, 45 -- BN1PR0401MB0977.namprd04.prod.outlook.com ([10.160.79.140]) with mapi id 9, 45 -- 15.01.0355.012; Mon, 21 Dec 2015 03:51:33 +0000 9, 45 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 9, 45 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 45 -- Subject: Out-of-office flood 9, 45 -- Thread-Topic: Out-of-office flood 9, 45 -- Thread-Index: AdE7ovgQyi7JDcIsRX+2zewgQZ+U3Q== 9, 45 -- Date: Mon, 21 Dec 2015 03:51:33 +0000 9, 45 -- Message-ID: {BN1PR0401MB097790624C6D29D8B53EF1E3D7E40-at-BN1PR0401MB0977.namprd04.prod.outlook.com} 9, 45 -- Accept-Language: en-US 9, 45 -- Content-Language: en-US 9, 45 -- X-MS-Has-Attach: 9, 45 -- X-MS-TNEF-Correlator: 9, 45 -- authentication-results: spf=none (sender IP is ) 9, 45 -- smtp.mailfrom=wesaia-at-iastate.edu; 9, 45 -- x-originating-ip: [69.66.102.132] 9, 45 -- x-microsoft-exchange-diagnostics: 1;BN1PR0401MB0980;5:33RAQNSH4BpH+U7pfbYVnDs69UsxGfkI4uemrW4QKuochyrW9HsEq3eW0Fnux7LHCFJaIOxHGvf0j6SKLpb3YqpXXBYwgFVWwCHYLrmCC2oMZpwYeUGd8dx7i1pVYtkCfunXdq3YPbLH4FqOADBJAQ==;24:6XqT26K6HsHVAc1fShLPZpoh0Usg04X3FliJUxlghWQ2YV7c19TZ5AFgSk6mJs7Urj2NKyj2nDRvRQ2D9C8T0t+qOksPp5AWTfLPz/tRSMs= 9, 45 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(42134001)(42139001);SRVR:BN1PR0401MB0980; 9, 45 -- x-microsoft-antispam-prvs: {BN1PR0401MB0980CB08A63BA2B759FDF334D7E40-at-BN1PR0401MB0980.namprd04.prod.outlook.com} 9, 45 -- x-exchange-antispam-report-test: UriScan:; 9, 45 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(520078)(5005006)(8121501046)(3002001)(10201501046);SRVR:BN1PR0401MB0980;BCL:0;PCL:0;RULEID:;SRVR:BN1PR0401MB0980; 9, 45 -- x-forefront-prvs: 079756C6B9 9, 45 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(199003)(189002)(5004730100002)(5001960100002)(122556002)(40100003)(75432002)(33656002)(90282001)(230783001)(2351001)(66066001)(77096005)(88552001)(101416001)(2900100001)(229853001)(10400500002)(99286002)(89122001)(86362001)(450100001)(105586002)(5002640100001)(586003)(1220700001)(189998001)(92566002)(74316001)(5003600100002)(1096002)(106356001)(54356999)(102836003)(107886002)(3846002)(6116002)(2501003)(81156007)(87936001)(50986999)(110136002)(76576001)(97736004)(5008740100001);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR0401MB0980;H:BN1PR0401MB0977.namprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 9, 45 -- received-spf: None (protection.outlook.com: iastate.edu does not designate 9, 45 -- permitted sender hosts) 9, 45 -- spamdiagnosticoutput: 1:23 9, 45 -- spamdiagnosticmetadata: NSPM 9, 45 -- Content-Type: text/plain; charset="us-ascii" 9, 45 -- MIME-Version: 1.0 9, 45 -- X-OriginatorOrg: iastate.edu 9, 45 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 21 Dec 2015 03:51:33.0093 9, 45 -- (UTC) 9, 45 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 9, 45 -- X-MS-Exchange-CrossTenant-id: 0347d89a-0174-4dd3-adeb-3339c89c35f5 9, 45 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN1PR0401MB0980 9, 45 -- Content-Transfer-Encoding: 8bit 9, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tBL4Auik002496 ==============================End of - Headers==============================
RE: viaWWW:LM: linear measurement significant figures
I think I may have set a new personal record - 60 out-of-office automatic replies. I suppose I should have expected that as the holidays approach. I might have done even better by waiting until next week.
16 responses came from vendors, some of who I actually deal with. The others let me know anyway. ;) 31 responses came from various labs. I have dealings with none of them, although I recognized many respected institutions. 13 more came from organizations I could not recognize as vendors or labs. Of course, that means I have dealings with none of them.
The list FAQs state "Do not set your Email program to deliver "out of the office/vacation" messages. If you will be gone, you should unsubscribe from the list and subscribe again when you return." Please consider it, especially vendors. I know you would like to keep your name in front of your potential customers. However, it seems counterproductive when it is by way of one of these messages. Besides, it seems that some of you are always out of the office and on the road.
Regardless, I thank the posters from the past year. I appreciate you sharing of your time and wisdom.
And thank you, Nestor, for keeping this forum going.
Merry Christmas and a Happy New Year.
Warren
==============================Original Headers============================== 9, 45 -- From wesaia-at-iastate.edu Tue Dec 22 18:05:08 2015 9, 45 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1bon0086.outbound.protection.outlook.com [157.56.111.86]) 9, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBN054LC008614 9, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Dec 2015 18:05:04 -0600 9, 45 -- Received: from BN1PR0401MB0977.namprd04.prod.outlook.com (10.160.79.140) by 9, 45 -- BN1PR0401MB0979.namprd04.prod.outlook.com (10.160.79.142) with Microsoft SMTP 9, 45 -- Server (TLS) id 15.1.361.13; Mon, 21 Dec 2015 17:24:44 +0000 9, 45 -- Received: from BN1PR0401MB0977.namprd04.prod.outlook.com ([10.160.79.140]) by 9, 45 -- BN1PR0401MB0977.namprd04.prod.outlook.com ([10.160.79.140]) with mapi id 9, 45 -- 15.01.0355.012; Mon, 21 Dec 2015 17:24:44 +0000 9, 45 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 9, 45 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 45 -- Subject: Please do not flood our mailboxes 9, 45 -- Thread-Topic: Please do not flood our mailboxes 9, 45 -- Thread-Index: AdE8FHr0scveeud9T9eroz5MDmqEBg== 9, 45 -- Date: Mon, 21 Dec 2015 17:24:44 +0000 9, 45 -- Message-ID: {BN1PR0401MB097784D84FDD9814EADC8847D7E40-at-BN1PR0401MB0977.namprd04.prod.outlook.com} 9, 45 -- Accept-Language: en-US 9, 45 -- Content-Language: en-US 9, 45 -- X-MS-Has-Attach: 9, 45 -- X-MS-TNEF-Correlator: 9, 45 -- authentication-results: spf=none (sender IP is ) 9, 45 -- smtp.mailfrom=wesaia-at-iastate.edu; 9, 45 -- x-originating-ip: [129.186.7.36] 9, 45 -- x-microsoft-exchange-diagnostics: 1;BN1PR0401MB0979;5:v++uU9/Ju3sPH2xluGBBEmzTWRnILRz6ji1p/cbkwTYdwaqKp5A3MlW07ShKHjbbI1FJhe3cBV+aE3K293T3Oy8pIfgXeGRB0mQeNR4LMJBbQPHTXybAzmKrlOn/y84eWbxgxStuFcs0xAzHxtG7nw==;24:kvlo9R9CIHAXtRCN08R+av21M1+0lIDTvdJqvmaEXKghDwU3rV9YDLZkevzVnnYKuXp52PBguHGkgVXYDkBodlglr2WoJT1En1JQfjk856Y= 9, 45 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(42134001)(42139001);SRVR:BN1PR0401MB0979; 9, 45 -- x-microsoft-antispam-prvs: {BN1PR0401MB0979DC00C3F9B9E7C5C33AC5D7E40-at-BN1PR0401MB0979.namprd04.prod.outlook.com} 9, 45 -- x-exchange-antispam-report-test: UriScan:; 9, 45 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(520078)(5005006)(8121501046)(3002001)(10201501046);SRVR:BN1PR0401MB0979;BCL:0;PCL:0;RULEID:;SRVR:BN1PR0401MB0979; 9, 45 -- x-forefront-prvs: 079756C6B9 9, 45 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(189002)(199003)(87936001)(2501003)(5003600100002)(88552001)(110136002)(5001960100002)(450100001)(107886002)(5008740100001)(86362001)(33656002)(189998001)(99286002)(106356001)(5002640100001)(90282001)(97736004)(75432002)(50986999)(101416001)(66066001)(74316001)(76576001)(92566002)(229853001)(54356999)(81156007)(2900100001)(11100500001)(89122001)(122556002)(3846002)(40100003)(1220700001)(586003)(102836003)(5004730100002)(105586002)(77096005)(1096002)(10400500002)(6116002)(2351001);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR0401MB0979;H:BN1PR0401MB0977.namprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 9, 45 -- received-spf: None (protection.outlook.com: iastate.edu does not designate 9, 45 -- permitted sender hosts) 9, 45 -- spamdiagnosticoutput: 1:23 9, 45 -- spamdiagnosticmetadata: NSPM 9, 45 -- Content-Type: text/plain; charset="us-ascii" 9, 45 -- MIME-Version: 1.0 9, 45 -- X-OriginatorOrg: iastate.edu 9, 45 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 21 Dec 2015 17:24:44.7531 9, 45 -- (UTC) 9, 45 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 9, 45 -- X-MS-Exchange-CrossTenant-id: 0347d89a-0174-4dd3-adeb-3339c89c35f5 9, 45 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN1PR0401MB0979 9, 45 -- Content-Transfer-Encoding: 8bit 9, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tBN054LC008614 ==============================End of - Headers==============================
My EDX detector window for SEM is broken and I had problems with transport dammage when purchasing similar equipment from other countries. Does anyone in Spain or Portugal has a spare unused detector (LN2 cooled is OK) that can be acquired at low or no cost? I would be able to go somewhere in these countries and transport it under proper conditions.
Please contact me to apamatos-at-gmail.com
Marry Christmas and Happy New Year A.P. Alves de Matos Cmicros - Egas Moniz Centre for Histopathology and Electron Microscopy, Portugal
==============================Original Headers============================== 5, 34 -- From apamatos-at-gmail.com Thu Dec 24 07:43:52 2015 5, 34 -- Received: from mail-wm0-f49.google.com (mail-wm0-f49.google.com [74.125.82.49]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBODhqAV028210 5, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Dec 2015 07:43:52 -0600 5, 34 -- Received: by mail-wm0-f49.google.com with SMTP id p187so178618698wmp.0 5, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Dec 2015 05:43:44 -0800 (PST) 5, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 34 -- d=gmail.com; s=20120113; 5, 34 -- h=from:to:subject:message-id:date:user-agent:mime-version 5, 34 -- :content-type:content-transfer-encoding; 5, 34 -- bh=QiMnrU1lDo5Bap/NGvpAifafHlu5abx5hhl0C4CwQeU=; 5, 34 -- b=t8YfazD0pmr6BwHiAJTnUCXTB15iGW3uDAMJFbHQVfL48fBEziNJp1KiCHA/Qdpvta 5, 34 -- m50TYbf1X+gx3FtVGJZ1t8WVmDNWmFrAf0D2RYikRI/9lm4Z6lezeMaAVgNsG8Tsj72Z 5, 34 -- onUfq4H1xEIEmOQFxFb+Emmy1RX7sxkS5hvtVLxmJdihUayxaPKAlr1tCOWTpbIMHiFu 5, 34 -- Uw82MLgZpTp3uYV8YrhcZfxwT6z0YcWVBknegyaNPXthuQ6Nq/DHa71sPrdn0b1HqsTi 5, 34 -- hkBdUS/1tid8STajeQGKtxZio1BG0m0GSipACPmfA1a/lGiLGxePy3IwjweiN0fRO2g3 5, 34 -- JDbA== 5, 34 -- X-Received: by 10.28.137.138 with SMTP id l132mr34135442wmd.21.1450894783192; 5, 34 -- Wed, 23 Dec 2015 10:19:43 -0800 (PST) 5, 34 -- Received: from ?IPv6:2001:8a0:f946:ec01:4261:86ff:fe2b:8e36? ([2001:8a0:f946:ec01:4261:86ff:fe2b:8e36]) 5, 34 -- by smtp.gmail.com with ESMTPSA id pn6sm38418309wjb.15.2015.12.23.10.19.41 5, 34 -- for {Microscopy-at-microscopy.com} 5, 34 -- (version=TLSv1/SSLv3 cipher=OTHER); 5, 34 -- Wed, 23 Dec 2015 10:19:41 -0800 (PST) 5, 34 -- From: AP Alves de Matos {apamatos-at-gmail.com} 5, 34 -- To: Microscopy-at-microscopy.com 5, 34 -- Subject: SEM - Old (functional) EDX detector needed 5, 34 -- Message-ID: {567AE5BC.9010507-at-gmail.com} 5, 34 -- Date: Wed, 23 Dec 2015 18:19:40 +0000 5, 34 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101 5, 34 -- Thunderbird/38.4.0 5, 34 -- MIME-Version: 1.0 5, 34 -- Content-Type: text/plain; charset=utf-8; format=flowed 5, 34 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Society for Ultrastructural Pathology is organizing its XVIII Congress in Lisbon, Portugal from July 11 to 15, 2016. The program setup is now developing rapidly and you can check the overall schedule and further information at http://congress.ultrapathXVIII.org/program
The Congress will be preceeded by an introductory course on Ultrastructural Pathology organized by our senior and experienced Colleague Joseph Lloreta-Trull, from Hospital del Mar in Barcelona. Please check the proposed programme at http://congress.ultrapathXVIII.org/course.
This Congress will focus primarily on ultrastructural diagnosis. A comprehensive programme of invited speakers and submitted presentations will cover this topic. Sessions will be dedicated to the main diagnostic areas for which electron microscopy is important. Also more fundamental research and new technical advances, important for Ultrastructural Pathology as a whole, will be covered. New information will be posted frequently on the programme page as soon as it becomes confirmed.
The Congress and its associated Course is important for a wide range of participants, from medical doctors and Pathologists to research scientists dealing with Histology, Histopathology, Cell Biology etc. and also, most importantly, technicians active in these areas.**
Therefore it is our great pleasure to invite you to join us at UltrapathXVIII in the pleasant, friendly and inexpensive environment of Lisbon, and in the superb facilities of the Gulbenkian Foundation, where the Congress will be held.
Please help us to get the word out about this Congress by downloading the brochure from the link below and sending it to your colleagues.
On behalf of the organizing committee, I am looking forward to welcoming you to Lisbon in June 2016. The registration page is now on line. Please register at http://congress.ultrapathXVIII.org/registration
With kind regards,
From the Organizing Committee,
A.P. Alves de Matos Chair of UltrapathXVIII
==============================Original Headers============================== 20, 38 -- From apamatos-at-gmail.com Thu Dec 24 08:47:29 2015 20, 38 -- Received: from mail-wm0-f45.google.com (mail-wm0-f45.google.com [74.125.82.45]) 20, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBOElThS016955 20, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Dec 2015 08:47:29 -0600 20, 38 -- Received: by mail-wm0-f45.google.com with SMTP id p187so184991082wmp.0 20, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Dec 2015 06:47:21 -0800 (PST) 20, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 20, 38 -- d=gmail.com; s=20120113; 20, 38 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 20, 38 -- :in-reply-to:content-type:content-transfer-encoding; 20, 38 -- bh=YTEf3udZeeLYENU+fF59kKeIdLtAYzA1kvwthLKgTfA=; 20, 38 -- b=snc0gII4p6WvnjP/xnkITaXpm7tuHq+7IA+ggvFGmSsHu33Iyd+GQcKUdzPrB6zS9K 20, 38 -- u4kchBaFiDdSWvdHORQb/LQVQJ+l9uEsT4ViDOF8Oy80ts7qPqaATgb4uF+Eoexl6luN 20, 38 -- 6+KSuHj5s+FEGxhgvYmjCcjd2bAX/7dNo/+xhNv/BvA6HSs9agk5RmYnGSZ6FDwAD6Ji 20, 38 -- 9bRH9Ripc4S59ZcgEaJOhVXg+X0UCwj6uMb1Mv3M78g32gAGwfbg4txykyt51e+wuppN 20, 38 -- I0hFZhoFTRk9sfe2V8yhJXzExsCbZJtDxF7fAVM+lbOh0QEQhNTp0t1+Uq8qseO44otf 20, 38 -- vEKg== 20, 38 -- X-Received: by 10.194.88.73 with SMTP id be9mr23216731wjb.44.1450738659565; 20, 38 -- Mon, 21 Dec 2015 14:57:39 -0800 (PST) 20, 38 -- Received: from ?IPv6:2001:8a0:f946:ec01:4261:86ff:fe2b:8e36? ([2001:8a0:f946:ec01:4261:86ff:fe2b:8e36]) 20, 38 -- by smtp.gmail.com with ESMTPSA id ke10sm30107238wjb.3.2015.12.21.14.57.37 20, 38 -- for {Microscopy-at-microscopy.com} 20, 38 -- (version=TLSv1/SSLv3 cipher=OTHER); 20, 38 -- Mon, 21 Dec 2015 14:57:37 -0800 (PST) 20, 38 -- Subject: International Congress of the Society for Ultrastructural Pathology - 20, 38 -- UltrapathXVIII 20, 38 -- References: {567734F5.1030505-at-gmail.com} 20, 38 -- To: Microscopy-at-microscopy.com 20, 38 -- From: AP Alves de Matos {apamatos-at-gmail.com} 20, 38 -- X-Forwarded-Message-Id: {567734F5.1030505-at-gmail.com} 20, 38 -- Message-ID: {567883E0.6000904-at-gmail.com} 20, 38 -- Date: Mon, 21 Dec 2015 22:57:36 +0000 20, 38 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101 20, 38 -- Thunderbird/38.4.0 20, 38 -- MIME-Version: 1.0 20, 38 -- In-Reply-To: {567734F5.1030505-at-gmail.com} 20, 38 -- Content-Type: text/plain; charset=utf-8; format=flowed 20, 38 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Society for Ultrastructural Pathology is organizing its XVIII Congress in Lisbon, Portugal from July 11 to 15, 2016. The program setup is now developing rapidly and you can check the overall schedule and further information at http://congress.ultrapathXVIII.org/program
The Congress will be preceeded by an introductory course on Ultrastructural Pathology organized by our senior and experienced Colleague Joseph Lloreta-Trull, from Hospital del Mar in Barcelona. Please check the proposed programme at http://congress.ultrapathXVIII.org/course.
This Congress will focus primarily on ultrastructural diagnosis. A comprehensive programme of invited speakers and submitted presentations will cover this topic. Sessions will be dedicated to the main diagnostic areas for which electron microscopy is important. Also more fundamental research and new technical advances, important for Ultrastructural Pathology as a whole, will be covered. New information will be posted frequently on the programme page as soon as it becomes confirmed.
The Congress and its associated Course is important for a wide range of participants, from medical doctors and Pathologists to research scientists dealing with Histology, Histopathology, Cell Biology etc. and also, most importantly, technicians active in these areas.**
Therefore it is our great pleasure to invite you to join us at UltrapathXVIII in the pleasant, friendly and inexpensive environment of Lisbon, and in the superb facilities of the Gulbenkian Foundation, where the Congress will be held.
Please help us to get the word out about this Congress by downloading the brochure from the link below and sending it to your colleagues.
On behalf of the organizing committee, I am looking forward to welcoming you to Lisbon in June 2016. The registration page is now on line. Please register at http://congress.ultrapathXVIII.org/registration
With kind regards,
From the Organizing Committee,
A.P. Alves de Matos Chair of UltrapathXVIII
==============================Original Headers============================== 17, 38 -- From apamatos-at-gmail.com Thu Dec 24 08:57:10 2015 17, 38 -- Received: from mail-wm0-f45.google.com (mail-wm0-f45.google.com [74.125.82.45]) 17, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBOEv9ln022556 17, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Dec 2015 08:57:09 -0600 17, 38 -- Received: by mail-wm0-f45.google.com with SMTP id p187so182368648wmp.1 17, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Dec 2015 06:57:01 -0800 (PST) 17, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 38 -- d=gmail.com; s=20120113; 17, 38 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 17, 38 -- :in-reply-to:content-type:content-transfer-encoding; 17, 38 -- bh=b1nrzYCNecUdLyebx+t64xjXatQ4D0I0BkndxsVx+v0=; 17, 38 -- b=v0hCRRS3tyMWZjerGqLC6nviRwtQQqAQFAtL1intiSUKEvHd7Q4W2KFSLCr2bj2blZ 17, 38 -- G7Z5oz9GCJKt1wpw/l0Mazdmy85bsbw+w4TzFl/HwbDtijcXmI977hXo2CFspnjeQtwH 17, 38 -- x9p9B7vjed1DG2ERFsnkTropiS94muXKLQAO0wG713cydNVz5owpiaMT+ZOCQTybTPnb 17, 38 -- uVTyr/fXbTEcbyq5So7EoaXE3jzX51uMIEg3bG7xS36LH2IXpB9GQmkDBSJ2Kb3/U0jP 17, 38 -- XqGy2yTwIPZRbWfhQ1vryEgykhb1iMFtt4Z9RWrpxHLSlFUf2cjSdeCh+7BPE9sguwTD 17, 38 -- Tq6w== 17, 38 -- X-Received: by 10.194.7.100 with SMTP id i4mr22109654wja.76.1450824545912; 17, 38 -- Tue, 22 Dec 2015 14:49:05 -0800 (PST) 17, 38 -- Received: from ?IPv6:2001:8a0:f946:ec01:4261:86ff:fe2b:8e36? ([2001:8a0:f946:ec01:4261:86ff:fe2b:8e36]) 17, 38 -- by smtp.gmail.com with ESMTPSA id jo6sm34891168wjb.48.2015.12.22.14.49.03 17, 38 -- for {Microscopy-at-microscopy.com} 17, 38 -- (version=TLSv1/SSLv3 cipher=OTHER); 17, 38 -- Tue, 22 Dec 2015 14:49:04 -0800 (PST) 17, 38 -- Subject: International Congress of the Society for Ultrastructural Pathology - 17, 38 -- UltrapathXVIII 17, 38 -- References: {CAAH0jd0g73SY+beEJtV0ta_Ak3qp-RUcrzsTuR1O6NfgWrow2w-at-mail.gmail.com} 17, 38 -- To: Microscopy-at-microscopy.com 17, 38 -- From: AP Alves de Matos {apamatos-at-gmail.com} 17, 38 -- X-Forwarded-Message-Id: {CAAH0jd0g73SY+beEJtV0ta_Ak3qp-RUcrzsTuR1O6NfgWrow2w-at-mail.gmail.com} 17, 38 -- Message-ID: {5679D35F.8060702-at-gmail.com} 17, 38 -- Date: Tue, 22 Dec 2015 22:49:03 +0000 17, 38 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101 17, 38 -- Thunderbird/38.4.0 17, 38 -- MIME-Version: 1.0 17, 38 -- In-Reply-To: {CAAH0jd0g73SY+beEJtV0ta_Ak3qp-RUcrzsTuR1O6NfgWrow2w-at-mail.gmail.com} 17, 38 -- Content-Type: text/plain; charset=utf-8; format=flowed 17, 38 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Society for Ultrastructural Pathology is organizing its XVIII Congress in Lisbon, Portugal from July 11 to 15, 2016. The program setup is now developing rapidly and you can check the overall schedule and further information at http://congress.ultrapathXVIII.org/program
The Congress will be preceeded by an introductory course on Ultrastructural Pathology organized by our senior and experienced Colleague Joseph Lloreta-Trull, from Hospital del Mar in Barcelona. Please check the proposed programme at http://congress.ultrapathXVIII.org/course.
This Congress will focus primarily on ultrastructural diagnosis. A comprehensive programme of invited speakers and submitted presentations will cover this topic. Sessions will be dedicated to the main diagnostic areas for which electron microscopy is important. Also more fundamental research and new technical advances, important for Ultrastructural Pathology as a whole, will be covered. New information will be posted frequently on the programme page as soon as it becomes confirmed.
The Congress and its associated Course is important for a wide range of participants, from medical doctors and Pathologists to research scientists dealing with Histology, Histopathology, Cell Biology etc. and also, most importantly, technicians active in these areas.**
Therefore it is our great pleasure to invite you to join us at UltrapathXVIII in the pleasant, friendly and inexpensive environment of Lisbon, and in the superb facilities of the Gulbenkian Foundation, where the Congress will be held.
Please help us to get the word out about this Congress by downloading the brochure from the link below and sending it to your colleagues.
On behalf of the organizing committee, I am looking forward to welcoming you to Lisbon in June 2016. The registration page is now on line. Please register at http://congress.ultrapathXVIII.org/registration
With kind regards,
X-from the Organizing Committee,
A.P. Alves de Matos Chair of UltrapathXVIII
==============================Original Headers============================== 13, 26 -- From apamatos-at-gmail.com Thu Dec 24 09:31:51 2015 13, 26 -- Received: from mail-wm0-f44.google.com (mail-wm0-f44.google.com [74.125.82.44]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBOFVoF1025701 13, 26 -- for {microscopy-at-microscopy.com} ; Thu, 24 Dec 2015 09:31:51 -0600 13, 26 -- Received: by mail-wm0-f44.google.com with SMTP id l126so185143201wml.0 13, 26 -- for {microscopy-at-microscopy.com} ; Thu, 24 Dec 2015 07:31:42 -0800 (PST) 13, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 26 -- d=gmail.com; s=20120113; 13, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 13, 26 -- bh=Ekp5+6y4j3lc3b8WRq1pRDYlRFG8m7jNXaWXK5Qo/t4=; 13, 26 -- b=coC4IzcVUN6xCYS6aUimllrgip5xmm0orr0uPbqMc/oteceVMY3jl7f91W24y67qaa 13, 26 -- ZFfa530w6P1gEYKP720TK8IXXAoZvs1RvRz1/rZSNKbAldrEQ51V1kBrLqIX6tds3gzQ 13, 26 -- iLWpIcPx+lw3LZ4QxPhExhgAzS9t0ji7LLBtF0bYY3FLHxsNJXEajGmb1gXLFW68l+gb 13, 26 -- hQfCOUnY9uUobT0YFvPcB0N37BTgXe9W9d2rr9pGRGb6YBsqbHcpoPsCtnuGlicMgPi/ 13, 26 -- 3rAOj+0tY1ylI6KnM/wAlzmypv6HKbQS4NG9I6dcljvI7LqKhaTDCngSMeFHi0Cz19BA 13, 26 -- F7Ig== 13, 26 -- MIME-Version: 1.0 13, 26 -- X-Received: by 10.28.23.135 with SMTP id 129mr22346024wmx.11.1450739498001; 13, 26 -- Mon, 21 Dec 2015 15:11:38 -0800 (PST) 13, 26 -- Received: by 10.194.110.133 with HTTP; Mon, 21 Dec 2015 15:11:37 -0800 (PST) 13, 26 -- Date: Mon, 21 Dec 2015 23:11:37 +0000 13, 26 -- Message-ID: {CAAH0jd0g73SY+beEJtV0ta_Ak3qp-RUcrzsTuR1O6NfgWrow2w-at-mail.gmail.com} 13, 26 -- Subject: International Congress of the Society for Ultrastructural Pathology - UltrapathXVIII 13, 26 -- From: =?UTF-8?Q?Ant=C3=B3nio_Matos?= {apamatos-at-gmail.com} 13, 26 -- To: microscopy-at-microscopy.com 13, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Organization: KSU
Title-Subject: [Filtered] Error message in Gatan Digital micrograph.
Message: Hi, Wishing you all a merry Christmas and happy new year. I am getting an error message "RPC server is unavailable" while acquiring image on digital micrograph. How to correct this error.?
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==============================Original Headers============================== 16, 40 -- From microscopy.listserver-at-gmail.com Mon Dec 28 16:56:52 2015 16, 40 -- Received: from mail-io0-f176.google.com (mail-io0-f176.google.com [209.85.223.176]) 16, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBSMupbU026195 16, 40 -- for {microscopy-at-microscopy.com} ; Mon, 28 Dec 2015 16:56:51 -0600 16, 40 -- Received: by mail-io0-f176.google.com with SMTP id 77so5690081ioc.2 16, 40 -- for {microscopy-at-microscopy.com} ; Mon, 28 Dec 2015 14:56:43 -0800 (PST) 16, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 40 -- d=gmail.com; s=20120113; 16, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 16, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 16, 40 -- bh=nZEKGB8r7hpgQYBD9TIpn3AKMQka1rL2r8nhKkYUYJo=; 16, 40 -- b=jyJzxq+xVufZ80MXCfhTE13Hr9wPjdg9D27uRBI/TTKjVSEstwDKitR27CchQaa2ik 16, 40 -- vINULjXN7BVeBt/7EEDn1TUNBKn7vx4SbygVbjIu58fcE+5n3s4XwExE3pr5ta9neiE5 16, 40 -- WqLlz+E7n7tLBUB6hNrcioM0DJYLdNiFA0gQy+WHKHX4eqssErug9nIWJcO1/y/DVr2z 16, 40 -- PNsgCSF9HyJyCaBTSyGOtAQ+9HB3nf818yUOpZ1KqN2WEB/mg/pXJ6Ouri7FRiBbrdDT 16, 40 -- WLKnGGFRdR9yDmLYUT9ZCK2I0ExYMAADBA2G9b3ZMVHikMtFZPtJcuF0m9nq12jzdE33 16, 40 -- SUkQ== 16, 40 -- X-Received: by 10.107.154.67 with SMTP id c64mr51592740ioe.53.1451343402939; 16, 40 -- Mon, 28 Dec 2015 14:56:42 -0800 (PST) 16, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 16, 40 -- by smtp.googlemail.com with ESMTPSA id c5sm20486541igt.9.2015.12.28.14.56.42 16, 40 -- for {microscopy-at-microscopy.com} 16, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 16, 40 -- Mon, 28 Dec 2015 14:56:42 -0800 (PST) 16, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 16, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 40 -- {microscopylistserver-noreply-at-microscopy.com} 16, 40 -- Subject: viaWWW:RPC Error message in Gatan Digital micrograph. 16, 40 -- References: {201512282246.tBSMkD6q026083-at-ns.microscopy.com} 16, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 40 -- X-Forwarded-Message-Id: {201512282246.tBSMkD6q026083-at-ns.microscopy.com} 16, 40 -- Message-ID: {5681BE29.3070001-at-microscopy.com} 16, 40 -- Date: Mon, 28 Dec 2015 16:56:41 -0600 16, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 16, 40 -- Gecko/20100101 Thunderbird/38.4.0 16, 40 -- MIME-Version: 1.0 16, 40 -- In-Reply-To: {201512282246.tBSMkD6q026083-at-ns.microscopy.com} 16, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
anybody out who does have a Jeol 840 user manual and maybe also a service manual in PDF? I would appreciate it very much.
Best wishes for the upcoming 2016 Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Dear All, thanks; I got the manuals. No more need.
Best wishes for 2016 Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 29.12.15 um 15:27 schrieb stefan.diller-at-t-online.de: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } anybody out who does have a Jeol 840 user manual and maybe also a service manual in PDF? } I would appreciate it very much. } } Best wishes for the upcoming 2016 } Stefan }
==============================Original Headers============================== 6, 24 -- From stefan.diller-at-t-online.de Tue Dec 29 09:39:23 2015 6, 24 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBTFdNaR016474 6, 24 -- for {microscopy-at-microscopy.com} ; Tue, 29 Dec 2015 09:39:23 -0600 6, 24 -- Received: from fwd29.aul.t-online.de (fwd29.aul.t-online.de [172.20.26.134]) 6, 24 -- by mailout04.t-online.de (Postfix) with SMTP id 4BD034B6752 6, 24 -- for {microscopy-at-microscopy.com} ; Tue, 29 Dec 2015 16:39:14 +0100 (CET) 6, 24 -- Received: from mac-pro.fritz.box (bRdzoeZYwhX4rZEP9DfUookDd0rirdOQATvsuhU9JiueIIYkfOrb3yfSzDupZKMQqA-at-[91.58.170.103]) by fwd29.t-online.de 6, 24 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) 6, 24 -- esmtp id 1aDwMg-2K1mDY0; Tue, 29 Dec 2015 16:39:10 +0100 6, 24 -- Subject: Re: [Microscopy] Jeol 840 manuals 6, 24 -- References: {201512291427.tBTER3U0031941-at-ns.microscopy.com} 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- From: Stefan Diller {stefan.diller-at-t-online.de} 6, 24 -- Message-ID: {5682A91D.5070902-at-t-online.de} 6, 24 -- Date: Tue, 29 Dec 2015 16:39:09 +0100 6, 24 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 6, 24 -- Gecko/20100101 Thunderbird/38.4.0 6, 24 -- MIME-Version: 1.0 6, 24 -- In-Reply-To: {201512291427.tBTER3U0031941-at-ns.microscopy.com} 6, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 24 -- Content-Transfer-Encoding: 7bit 6, 24 -- X-ID: bRdzoeZYwhX4rZEP9DfUookDd0rirdOQATvsuhU9JiueIIYkfOrb3yfSzDupZKMQqA 6, 24 -- X-TOI-MSGID: 9b77f3ef-e8a3-4d40-b8fe-7e522f62aa74 ==============================End of - Headers==============================
From ohn.bullocknewssz-at-gmail.com Tue Dec 29 15:27:42 2015 Return-Path: {ohn.bullocknewssz-at-gmail.com} Received: from gmail.com (221-143-48-230.tongkni.co.kr [221.143.48.230] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id tBTLRdE0012799 for {microscopylistserverarchive5-at-microscopy.com} ; Tue, 29 Dec 2015 15:27:41 -0600 Message-ID: {DAFD8062.24363948-at-gmail.com}
X-from: tori9308-at-gmail.com
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Email: tori9308-at-gmail.com Name: Tori Harrington
Organization: none
Title-Subject: [Filtered] books to donate
Message: I have 3 books of TEM ultrastructure of cells and tissues. I will be happy to send them to anyone who wants them for the price of postage.
"A Study Atlas of Electron Micrographs" Judy M. Strum 1992, 3rd edition
"Fine Structure of Cells and Tissues" Porter and Bonneville, 4th edition
"Functional Ultrastructure, Atlas of Tissue Biology and Pathology" Pavelka and Roth, 3rd edition
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Wed Dec 30 06:34:24 2015 14, 40 -- Received: from mail-ig0-f175.google.com (mail-ig0-f175.google.com [209.85.213.175]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBUCYOLR023085 14, 40 -- for {microscopy-at-microscopy.com} ; Wed, 30 Dec 2015 06:34:24 -0600 14, 40 -- Received: by mail-ig0-f175.google.com with SMTP id mw1so50327134igb.1 14, 40 -- for {microscopy-at-microscopy.com} ; Wed, 30 Dec 2015 04:34:15 -0800 (PST) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=Z7DggRMDRuPKgahHaoJj0gcftFYcrwXva8DhcKOlv/k=; 14, 40 -- b=Oj8RUPVIERpep29eb0a8I8U8ZDeOfmnJ7pNlonL3OSJg4fWitofjD+9FrQSFBvDPPq 14, 40 -- RpBEZFHPrnG6thUesGHH3ugjuR5G81ifdqqWUqPkPtpj0Sj1tcUOEGIgHS6oZng72/b+ 14, 40 -- W7sdLSJkcGaHMrkQFjJsMvUkOZ1zQf/71FDABvvRyGbMt2GVGoCukuk3kmANBfE7ju0G 14, 40 -- nHATXBXSIqsm3XYEk3uYmABNm2gP+uBuzIOVmex0pNzr8xC9KO1D5jzIA8z5We69EJfX 14, 40 -- 1mVpP5A0Nk5VhGz8bN/vva1kR1hrJREjz5Kt5rXVmTMkiAAoo8EHAjH+EEf+DKU/Jjlu 14, 40 -- F0UQ== 14, 40 -- X-Received: by 10.50.78.101 with SMTP id a5mr59445667igx.42.1451478855438; 14, 40 -- Wed, 30 Dec 2015 04:34:15 -0800 (PST) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id ki7sm22692177igb.2.2015.12.30.04.34.14 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 14, 40 -- Wed, 30 Dec 2015 04:34:14 -0800 (PST) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:EM books to donate 14, 40 -- References: {201512292120.tBTLKCkJ012682-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201512292120.tBTLKCkJ012682-at-ns.microscopy.com} 14, 40 -- Message-ID: {5683CF46.90505-at-microscopy.com} 14, 40 -- Date: Wed, 30 Dec 2015 06:34:14 -0600 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.5.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201512292120.tBTLKCkJ012682-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: bryan-at-systap.com Name: Bryan Thompson
Title-Subject: [Filtered] Preparation of beach coral for SEM?
Message: Hello,
I brought back a piece of coral from a beach recently. I am wondering how people would suggest drying out this sample. The sample was "found" on the beach (wet, wave tossed). My goal is uncoated observation in SEM.
I could: - bake it - leave it in a vacuum chamber - run it through a CPD.
CPD seems like overkill given that this was a loose / dead sample, not a piece of living coral.
Thanks, Bryan
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==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Wed Dec 30 06:35:15 2015 14, 40 -- Received: from mail-io0-f171.google.com (mail-io0-f171.google.com [209.85.223.171]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBUCZFjG023238 14, 40 -- for {microscopy-at-microscopy.com} ; Wed, 30 Dec 2015 06:35:15 -0600 14, 40 -- Received: by mail-io0-f171.google.com with SMTP id q21so67427900iod.0 14, 40 -- for {microscopy-at-microscopy.com} ; Wed, 30 Dec 2015 04:35:06 -0800 (PST) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=iJjr8e1fEJFGvNCReAFu7S3nm5no0ib++sF3T9dupQw=; 14, 40 -- b=gpFIjvb3CEnAGyJM6kTOfv2volQUwuvBYfpkOAiho9O5TYp7xp7oQwZ4k+dNUIVAhh 14, 40 -- Q6gYvIbNRmj6G0NWv20a++wQq5a7tJ4xRu+AfwmNHWnJJukG30/5ncY6ysqHTudgy21g 14, 40 -- FEmRS/9SolH+cOQ/o019q3M0MYdz3Ov1P1G8Vj8CDrmP/vR0pTgkhilHmbfjKBDxaDYo 14, 40 -- fBc53mMqL+oHBZ2q5BRcbiYSGPVgNR8eSAS0qK1j4Fb3TeZ26wTR4mmPOp19ZSCdxydK 14, 40 -- CakBj9EGQ6tD3bgelF5e9oLzn4tihiqpssmYCpvaiXPzI/RKAf9Ol1gPoDkx3dZ9//gZ 14, 40 -- yYdA== 14, 40 -- X-Received: by 10.107.128.133 with SMTP id k5mr58524097ioi.26.1451478906509; 14, 40 -- Wed, 30 Dec 2015 04:35:06 -0800 (PST) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id g5sm22802508igc.9.2015.12.30.04.35.05 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 14, 40 -- Wed, 30 Dec 2015 04:35:06 -0800 (PST) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:Preparation of beach coral for SEM? 14, 40 -- References: {201512300023.tBU0NdCJ015247-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201512300023.tBU0NdCJ015247-at-ns.microscopy.com} 14, 40 -- Message-ID: {5683CF79.4070708-at-microscopy.com} 14, 40 -- Date: Wed, 30 Dec 2015 06:35:05 -0600 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.5.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201512300023.tBU0NdCJ015247-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You can try to treat it with a bleach for 15 min to get rid of unwanted organic stuff, wash and air dry it. I do quite often for bone and teeth. Good luck. Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, December 30, 2015 6:36 AM To: Dusevich, Vladimir
X-from: bryan-at-systap.com
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Email: bryan-at-systap.com Name: Bryan Thompson
Title-Subject: [Filtered] Preparation of beach coral for SEM?
Message: Hello,
I brought back a piece of coral from a beach recently. I am wondering how people would suggest drying out this sample. The sample was "found" on the beach (wet, wave tossed). My goal is uncoated observation in SEM.
I could: - bake it - leave it in a vacuum chamber - run it through a CPD.
CPD seems like overkill given that this was a loose / dead sample, not a piece of living coral.
Thanks, Bryan
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============================================
==============================Original Headers============================== 14, 40 -- From microscopy.listserver-at-gmail.com Wed Dec 30 06:35:15 2015 14, 40 -- Received: from mail-io0-f171.google.com (mail-io0-f171.google.com [209.85.223.171]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBUCZFjG023238 14, 40 -- for {microscopy-at-microscopy.com} ; Wed, 30 Dec 2015 06:35:15 -0600 14, 40 -- Received: by mail-io0-f171.google.com with SMTP id q21so67427900iod.0 14, 40 -- for {microscopy-at-microscopy.com} ; Wed, 30 Dec 2015 04:35:06 -0800 (PST) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=20120113; 14, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 14, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 14, 40 -- bh=iJjr8e1fEJFGvNCReAFu7S3nm5no0ib++sF3T9dupQw=; 14, 40 -- b=gpFIjvb3CEnAGyJM6kTOfv2volQUwuvBYfpkOAiho9O5TYp7xp7oQwZ4k+dNUIVAhh 14, 40 -- Q6gYvIbNRmj6G0NWv20a++wQq5a7tJ4xRu+AfwmNHWnJJukG30/5ncY6ysqHTudgy21g 14, 40 -- FEmRS/9SolH+cOQ/o019q3M0MYdz3Ov1P1G8Vj8CDrmP/vR0pTgkhilHmbfjKBDxaDYo 14, 40 -- fBc53mMqL+oHBZ2q5BRcbiYSGPVgNR8eSAS0qK1j4Fb3TeZ26wTR4mmPOp19ZSCdxydK 14, 40 -- CakBj9EGQ6tD3bgelF5e9oLzn4tihiqpssmYCpvaiXPzI/RKAf9Ol1gPoDkx3dZ9//gZ 14, 40 -- yYdA== 14, 40 -- X-Received: by 10.107.128.133 with SMTP id k5mr58524097ioi.26.1451478906509; 14, 40 -- Wed, 30 Dec 2015 04:35:06 -0800 (PST) 14, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 14, 40 -- by smtp.googlemail.com with ESMTPSA id g5sm22802508igc.9.2015.12.30.04.35.05 14, 40 -- for {microscopy-at-microscopy.com} 14, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 14, 40 -- Wed, 30 Dec 2015 04:35:06 -0800 (PST) 14, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 40 -- {microscopylistserver-noreply-at-microscopy.com} 14, 40 -- Subject: viaWWW:Preparation of beach coral for SEM? 14, 40 -- References: {201512300023.tBU0NdCJ015247-at-ns.microscopy.com} 14, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 40 -- X-Forwarded-Message-Id: {201512300023.tBU0NdCJ015247-at-ns.microscopy.com} 14, 40 -- Message-ID: {5683CF79.4070708-at-microscopy.com} 14, 40 -- Date: Wed, 30 Dec 2015 06:35:05 -0600 14, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 14, 40 -- Gecko/20100101 Thunderbird/38.5.0 14, 40 -- MIME-Version: 1.0 14, 40 -- In-Reply-To: {201512300023.tBU0NdCJ015247-at-ns.microscopy.com} 14, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 22, 30 -- From DusevichV-at-umkc.edu Wed Dec 30 11:10:08 2015 22, 30 -- Received: from um-nip4-umkc-out.um.umsystem.edu (um-nip4-umkc-out.um.umsystem.edu [198.209.49.180]) 22, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id tBUHA8wJ003162 22, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Dec 2015 11:10:08 -0600 22, 30 -- X-IronPort-Anti-Spam-Filtered: true 22, 30 -- X-IronPort-Anti-Spam-Result: A2A4BQDNDoRW/9SeoM9bA4M6Um0GiFO0RBmBZBgKhW0CgRQ5EwEBAQEBAQF/C4Q0AQEBBDoUNwQCAQgRAwEBAQsCCgEHCQchEAEUCQgCBAoJCBUEh3kDEg67Xw1GAYJXAQEBAQEBAQEBAQEBAQEBAQEBAQEBGIZWhH+CTw0aAYEvEAIBHiERFQEBghlNG4EbBYdehVuJTQGFP4VIUYNUSoN8iFmGYYdZIwFAghEcgV1yAYNKBxcjAYEHAQEB 22, 30 -- X-IPAS-Result: A2A4BQDNDoRW/9SeoM9bA4M6Um0GiFO0RBmBZBgKhW0CgRQ5EwEBAQEBAQF/C4Q0AQEBBDoUNwQCAQgRAwEBAQsCCgEHCQchEAEUCQgCBAoJCBUEh3kDEg67Xw1GAYJXAQEBAQEBAQEBAQEBAQEBAQEBAQEBGIZWhH+CTw0aAYEvEAIBHiERFQEBghlNG4EbBYdehVuJTQGFP4VIUYNUSoN8iFmGYYdZIwFAghEcgV1yAYNKBxcjAYEHAQEB 22, 30 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 22, 30 -- by um-nip4-exch-relay.um.umsystem.edu with ESMTP; 30 Dec 2015 11:09:58 -0600 22, 30 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.202]) by 22, 30 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0266.001; 22, 30 -- Wed, 30 Dec 2015 11:09:58 -0600 22, 30 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 22, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 22, 30 -- Subject: RE: [Microscopy] viaWWW:Preparation of beach coral for SEM? 22, 30 -- Thread-Topic: [Microscopy] viaWWW:Preparation of beach coral for SEM? 22, 30 -- Thread-Index: AQHRQv6zejWDl5TWZk2BsLufbqRZ257jwv3w 22, 30 -- Date: Wed, 30 Dec 2015 17:09:57 +0000 22, 30 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DCD3DFE-at-UM-MBX-T01.um.umsystem.edu} 22, 30 -- References: {201512301236.tBUCaN6H024349-at-ns.microscopy.com} 22, 30 -- In-Reply-To: {201512301236.tBUCaN6H024349-at-ns.microscopy.com} 22, 30 -- Accept-Language: en-US 22, 30 -- Content-Language: en-US 22, 30 -- X-MS-Has-Attach: 22, 30 -- X-MS-TNEF-Correlator: 22, 30 -- x-originating-ip: [134.193.156.38] 22, 30 -- Content-Type: text/plain; charset="us-ascii" 22, 30 -- MIME-Version: 1.0 22, 30 -- Content-Transfer-Encoding: 8bit 22, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id tBUHA8wJ003162 ==============================End of - Headers==============================
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Email: tori9308-at-gmail.com Name: Tori Harrington
Organization: none
Title-Subject: [Filtered] Sorry everyone, the books are gone!
Message: Hi,
The three books I posted yesterday have found their new home. Let's hear it for the listserver, and this Great Service that Nestor provides!!
Tori
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