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==============================Original Headers============================== 20, 14 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 1 10:04:20 2015 20, 14 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 20, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t01G4JjG016147 20, 14 -- for {microscopy-at-microscopy.com} ; Thu, 1 Jan 2015 10:04:20 -0600 20, 14 -- Message-ID: {54A57004.1030702-at-microscopy.com} 20, 14 -- Date: Thu, 01 Jan 2015 10:04:20 -0600 20, 14 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 14 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 14 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 20, 14 -- MIME-Version: 1.0 20, 14 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 14 -- Subject: Administrivia: Happy New Year 2015 20, 14 -- Content-Type: text/plain; charset=utf-8; format=flowed 20, 14 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Offline TIA viewer for windows.
Message: How to see .emi and .ser file on windows.? Is there any offline TIA viewer for windows available.?
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==============================Original Headers============================== 11, 17 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 2 18:33:07 2015 11, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t030X73P019812 11, 17 -- for {microscopy-at-microscopy.com} ; Fri, 2 Jan 2015 18:33:07 -0600 11, 17 -- Message-ID: {54A738C3.8090206-at-microscopy.com} 11, 17 -- Date: Fri, 02 Jan 2015 18:33:07 -0600 11, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 11, 17 -- MIME-Version: 1.0 11, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 17 -- Subject: viaWWW:Offline TIA viewer for windows 11, 17 -- References: {201501021738.t02HchTB029960-at-ns.microscopy.com} 11, 17 -- In-Reply-To: {201501021738.t02HchTB029960-at-ns.microscopy.com} 11, 17 -- X-Forwarded-Message-Id: {201501021738.t02HchTB029960-at-ns.microscopy.com} 11, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This won't help entirely, but ImageJ has a plugin which can open the .SER files. You need to be sure that you point to the correct file which contains the image. Most of the experimental information is stored in the .EMI file.
The FEI website has a link to an off-line version of ESVision (http://www.fei.com/service-support/es-vision/) which is the original version of TIA. You may be able to run it and analyze the data directly. (I've not tested it.)
Good luck, Henk
On 1/2/2015 7:35 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: ravi.thakkar369-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com } Name: Ravi } } Title-Subject: [Filtered] Offline TIA viewer for windows. } } Message: How to see .emi and .ser file on windows.? } Is there any offline TIA viewer for windows available.? } } Login Host: 129.130.146.69 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
When coming back from holidays, I found our CM10's monitor display was completely dark :( A small part of data display on a corner of the screen has been disappeared (fainted) for a while but the machine was operational without any problem. Now the whole screen was black out. The Data Dim and Panel Dim knobs were usually set at low or off during non-usage times. Other panel lights are all normal.
It seems like a small part (bulb or circuit board?) wearing out. Is there any simple way to check or order/replay the part?
Your expertise on this is much appreciated. Thanks you in advance.
Guosheng Liu University of Saskatchewan Canada
==============================Original Headers============================== 6, 36 -- From guosheng.liu-at-usask.ca Mon Jan 5 14:39:36 2015 6, 36 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t05Kda7Y018234 6, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 14:39:36 -0600 6, 36 -- Received: from conversion-daemon.usask.ca by usask.ca 6, 36 -- (Oracle Communications Messaging Server 7.0.5.34.0 64bit (built Oct 14 2014)) 6, 36 -- id {0NHQ007001CC0Q00-at-usask.ca} for Microscopy-at-microscopy.com; Mon, 6, 36 -- 05 Jan 2015 14:39:23 -0600 (CST) 6, 36 -- Received: from CAMPUSCAS4.usask.ca (campuscas4.usask.ca [128.233.194.195]) 6, 36 -- by usask.ca 6, 36 -- (Oracle Communications Messaging Server 7.0.5.34.0 64bit (built Oct 14 2014)) 6, 36 -- with ESMTPS id {0NHQ0042E1DNL560-at-usask.ca} for Microscopy-at-microscopy.com; Mon, 6, 36 -- 05 Jan 2015 14:39:23 -0600 (CST) 6, 36 -- Received: from CAMPUSMB4.usask.ca ([fe80::f90b:9124:ecc1:45ed]) 6, 36 -- by CAMPUSCAS4.usask.ca ([::1]) with mapi id 14.03.0195.001; Mon, 6, 36 -- 5 Jan 2015 14:39:22 -0600 6, 36 -- Date: Mon, 05 Jan 2015 20:39:21 +0000 6, 36 -- From: "Liu, Guosheng" {guosheng.liu-at-usask.ca} 6, 36 -- Subject: The data screen/monitor turns black in our Philips CM10 TEM---need 6, 36 -- help. 6, 36 -- X-Originating-IP: [10.65.80.9] 6, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 36 -- Message-id: {569262A9A49BF84695B7BFAE3D8E2A201795B657-at-CAMPUSMB4.usask.ca} 6, 36 -- MIME-version: 1.0 6, 36 -- Content-type: text/plain; charset=us-ascii 6, 36 -- Content-language: en-US 6, 36 -- Accept-Language: en-US 6, 36 -- Thread-topic: The data screen/monitor turns black in our Philips CM10 6, 36 -- TEM---need help. 6, 36 -- Thread-index: AdApJ687OIaT+yDFR1KAXCrWuAYUFQ== 6, 36 -- X-MS-Has-Attach: 6, 36 -- X-MS-TNEF-Correlator: 6, 36 -- x-pmwin-version: 3.1.2.0, Antivirus-Engine: 3.53.0, Antivirus-Data: 5.09 6, 36 -- x-puremessage: [Scanned] 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id t05Kda7Y018234 ==============================End of - Headers==============================
One thing you can try is to shine a bright light up in the upper left corner of the Data Monitor and see if the characters show up. The Data monitor intensity circuitry is a little strange. When you adjust the Data Dim knob it controls the intensity of a bulb on a circuit board behind the panel. There is a light absorbing diode that is next to the bulb which measures the intensity and adjust the circuitry that controls the Data brightness. If this bulb is burned out, then the monitor goes black.
Good Luck, John
-----Original Message----- X-from: guosheng.liu-at-usask.ca [mailto:guosheng.liu-at-usask.ca] Sent: Monday, January 05, 2015 3:58 PM To: John J. Schreiber
Happy new year EM community!
When coming back from holidays, I found our CM10's monitor display was completely dark :( A small part of data display on a corner of the screen has been disappeared (fainted) for a while but the machine was operational without any problem. Now the whole screen was black out. The Data Dim and Panel Dim knobs were usually set at low or off during non-usage times. Other panel lights are all normal.
It seems like a small part (bulb or circuit board?) wearing out. Is there any simple way to check or order/replay the part?
Your expertise on this is much appreciated. Thanks you in advance.
Guosheng Liu University of Saskatchewan Canada
==============================Original Headers============================== 6, 36 -- From guosheng.liu-at-usask.ca Mon Jan 5 14:39:36 2015 6, 36 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t05Kda7Y018234 6, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 14:39:36 -0600 6, 36 -- Received: from conversion-daemon.usask.ca by usask.ca 6, 36 -- (Oracle Communications Messaging Server 7.0.5.34.0 64bit (built Oct 14 2014)) 6, 36 -- id {0NHQ007001CC0Q00-at-usask.ca} for Microscopy-at-microscopy.com; Mon, 6, 36 -- 05 Jan 2015 14:39:23 -0600 (CST) 6, 36 -- Received: from CAMPUSCAS4.usask.ca (campuscas4.usask.ca [128.233.194.195]) 6, 36 -- by usask.ca 6, 36 -- (Oracle Communications Messaging Server 7.0.5.34.0 64bit (built Oct 14 2014)) 6, 36 -- with ESMTPS id {0NHQ0042E1DNL560-at-usask.ca} for Microscopy-at-microscopy.com; Mon, 6, 36 -- 05 Jan 2015 14:39:23 -0600 (CST) 6, 36 -- Received: from CAMPUSMB4.usask.ca ([fe80::f90b:9124:ecc1:45ed]) 6, 36 -- by CAMPUSCAS4.usask.ca ([::1]) with mapi id 14.03.0195.001; Mon, 6, 36 -- 5 Jan 2015 14:39:22 -0600 6, 36 -- Date: Mon, 05 Jan 2015 20:39:21 +0000 6, 36 -- From: "Liu, Guosheng" {guosheng.liu-at-usask.ca} 6, 36 -- Subject: The data screen/monitor turns black in our Philips CM10 TEM---need 6, 36 -- help. 6, 36 -- X-Originating-IP: [10.65.80.9] 6, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 36 -- Message-id: {569262A9A49BF84695B7BFAE3D8E2A201795B657-at-CAMPUSMB4.usask.ca} 6, 36 -- MIME-version: 1.0 6, 36 -- Content-type: text/plain; charset=us-ascii 6, 36 -- Content-language: en-US 6, 36 -- Accept-Language: en-US 6, 36 -- Thread-topic: The data screen/monitor turns black in our Philips CM10 6, 36 -- TEM---need help. 6, 36 -- Thread-index: AdApJ687OIaT+yDFR1KAXCrWuAYUFQ== 6, 36 -- X-MS-Has-Attach: 6, 36 -- X-MS-TNEF-Correlator: 6, 36 -- x-pmwin-version: 3.1.2.0, Antivirus-Engine: 3.53.0, Antivirus-Data: 5.09 6, 36 -- x-puremessage: [Scanned] 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id t05Kda7Y018234 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 41 -- From js51-at-princeton.edu Mon Jan 5 15:13:00 2015 17, 41 -- Received: from Princeton.EDU (ppa03.Princeton.EDU [128.112.128.214]) 17, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t05LCxv5007434 17, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 15:13:00 -0600 17, 41 -- Received: from csgsmtp201l.Princeton.EDU (csgsmtp201l.Princeton.EDU [128.112.134.60]) 17, 41 -- by ppa03.princeton.edu (8.14.5/8.14.5) with ESMTP id t05LCwte008008 17, 41 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 17, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 16:12:59 -0500 17, 41 -- Received: from CSGHUB209W.pu.win.princeton.edu (csghub209w.Princeton.EDU [128.112.128.68]) 17, 41 -- by csgsmtp201l.Princeton.EDU (8.13.8/8.12.9) with ESMTP id t05LCw95028402; 17, 41 -- Mon, 5 Jan 2015 16:12:58 -0500 17, 41 -- Received: from CSGMBX204W.pu.win.princeton.edu ([169.254.5.107]) by 17, 41 -- CSGHUB209W.pu.win.princeton.edu ([128.112.128.68]) with mapi id 17, 41 -- 14.03.0174.001; Mon, 5 Jan 2015 16:12:58 -0500 17, 41 -- From: "John J. Schreiber" {js51-at-princeton.edu} 17, 41 -- To: "guosheng.liu-at-usask.ca" {guosheng.liu-at-usask.ca} 17, 41 -- CC: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 17, 41 -- Subject: RE: [Microscopy] The data screen/monitor turns black in our Philips 17, 41 -- CM10 TEM---need 17, 41 -- Thread-Topic: [Microscopy] The data screen/monitor turns black in our 17, 41 -- Philips CM10 TEM---need 17, 41 -- Thread-Index: AQHQKSpaN5c4gssm1kytWb/5J777E5yyBEEg 17, 41 -- Date: Mon, 5 Jan 2015 21:12:57 +0000 17, 41 -- Message-ID: {3C628C43B600C14EB700249D43D718D30E7497B9-at-CSGMBX204W.pu.win.princeton.edu} 17, 41 -- References: {201501052058.t05KwQuc005842-at-ns.microscopy.com} 17, 41 -- In-Reply-To: {201501052058.t05KwQuc005842-at-ns.microscopy.com} 17, 41 -- Accept-Language: en-US 17, 41 -- Content-Language: en-US 17, 41 -- X-MS-Has-Attach: 17, 41 -- X-MS-TNEF-Correlator: 17, 41 -- x-originating-ip: [128.112.141.39] 17, 41 -- Content-Type: text/plain; charset="us-ascii" 17, 41 -- MIME-Version: 1.0 17, 41 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.13.68,1.0.33,0.0.0000 17, 41 -- definitions=2015-01-05_04:2015-01-05,2015-01-05,1970-01-01 signatures=0 17, 41 -- X-Proofpoint-Spam-Details: rule=quarantine_notspam policy=quarantine score=0 spamscore=0 17, 41 -- suspectscore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 17, 41 -- adjust=0 reason=mlx scancount=1 engine=7.0.1-1402240000 17, 41 -- definitions=main-1501050208 17, 41 -- Content-Transfer-Encoding: 8bit 17, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t05LCxv5007434 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both roy.geiss-at-colostate.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: roy.geiss-at-colostate.edu Name: Roy H. Geiss
Organization: Colorado State University
Title-Subject: [Filtered] Summer School on Electron Diffraction
Message: We are happy to announce the second edition of the CIF Summer School, which this year will feature a 3-day workshop on electron diffraction methods for materials analysis (May 19-21, 2015). Registration will open mid-January to both CSU and non-CSU students and researchers. For more information, go to: http://cif.colostate.edu/cif-summer-school/.
Thank you. Regards, Karolien
Karolien Denef Associate Director/Research Scientist Central Instrument Facility (CIF) Department of Chemistry - Colorado State University Fort Collins, CO 80523-1872 970-491- 3832 (o); 970-556-4846 (cell) http://cif.colostate.edu/
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==============================Original Headers============================== 17, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 5 20:04:40 2015 17, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 17, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0624eGX004992 17, 17 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 20:04:40 -0600 17, 17 -- Message-ID: {54AB42B8.30808-at-microscopy.com} 17, 17 -- Date: Mon, 05 Jan 2015 20:04:40 -0600 17, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 17, 17 -- MIME-Version: 1.0 17, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 17 -- Subject: viaWWW:CSU Summer School on Electron Diffraction 17, 17 -- References: {201501051905.t05J5twj015449-at-ns.microscopy.com} 17, 17 -- In-Reply-To: {201501051905.t05J5twj015449-at-ns.microscopy.com} 17, 17 -- X-Forwarded-Message-Id: {201501051905.t05J5twj015449-at-ns.microscopy.com} 17, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jennifer.korrell-at-nih.gov Name: Jennifer Korrell
Message: Leidos Biomedical Research Inc. is seeking a dedicated, driven Research Associate II to join their Electron Microscopy Laboratory. This position is located in Frederick, MD.
Below is a link that will take you directly to the job description on our website. If interested, please apply using the "apply" button at the bottom of the page.
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==============================Original Headers============================== 16, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 5 20:05:27 2015 16, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0625Rlo005829 16, 17 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 20:05:27 -0600 16, 17 -- Message-ID: {54AB42E7.4080805-at-microscopy.com} 16, 17 -- Date: Mon, 05 Jan 2015 20:05:27 -0600 16, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 16, 17 -- MIME-Version: 1.0 16, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 17 -- Subject: viaWWW:Job Opportunity- Leidos Biomed 16, 17 -- References: {201501052108.t05L8Zbv007387-at-ns.microscopy.com} 16, 17 -- In-Reply-To: {201501052108.t05L8Zbv007387-at-ns.microscopy.com} 16, 17 -- X-Forwarded-Message-Id: {201501052108.t05L8Zbv007387-at-ns.microscopy.com} 16, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am in the early stages of my career as a facility director and was wondering if you could lend some tips for negotiating service contract prices.
Our facility has a TEM and SEM, both of which are under service contracts with FEI. The increase in price has been pretty steady over the years, i.e. ~$200 for the SEM and ~$500 for the TEM. This year, however, the increase in price for the SEM contract is ~$1,300!! This was even after the mutli-contract discount.
I did not expect such an increase this year so my budget was not adjusted accordingly. I would say that I was pretty unhappy with the SEM service this past year. Despite contacting my field service engineer multiple times, I never heard back or it took over a month to hear back. We are also still having low vac problems that have resulted in countless hours of down time. Should I mention these points to my sales rep?
I'm new to the whole business side of science and I am wondering if anyone has any suggestions. I wouldn't want to say something wrong.....
Thank you in advance!
-Blanca
==============================Original Headers============================== 7, 28 -- From bicarbaj-at-mtholyoke.edu Mon Jan 5 21:02:20 2015 7, 28 -- Received: from mail-qa0-f43.google.com (mail-qa0-f43.google.com [209.85.216.43]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0632K9S016234 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2015 21:02:20 -0600 7, 28 -- Received: by mail-qa0-f43.google.com with SMTP id n4so12813048qaq.30 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 05 Jan 2015 19:02:19 -0800 (PST) 7, 28 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 28 -- d=1e100.net; s=20130820; 7, 28 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to 7, 28 -- :content-type; 7, 28 -- bh=+gOabxjl/kKejBr9QtA8GHwB8BxOpFPF1/e5j2kL9ug=; 7, 28 -- b=Amkbww+76aocR+RPffhMCeCoHRQ+QhDbSYD2qLBMCZ6sGmrefz4FlNzWM/cdVYHCsy 7, 28 -- S8mupsgCRTxN86MhrXyBjpQ6NaSlqhvoUBxNxo1c/I3nOleS8Pk5Dt0qrZCNY+fIQOcV 7, 28 -- kP+Y73GsFNMyWJlb064vsNm4X9R+HKp2GWocQyPgBRs1tyEY1kQgc3xHSB46qXfbWPdk 7, 28 -- tLuEu3wOcTWBh5dowZO9QmF2cJXAyHvz7LYWrBP5OpVf4i7fthSh7lxmJdO9y9IklgpL 7, 28 -- obgCmL3hxyAbqan/x//k8I3BkUmi0ABfAs9dIU2s4VT9aXQSao9y2KfV+gIY54zgYUTF 7, 28 -- mLnA== 7, 28 -- X-Gm-Message-State: ALoCoQmYZLxpHyfvhreduK0PvHAFDvvWolUnN1G8IAMT2d++xBtEYy2GyBeI95LgGgCRdacNnoQ4 7, 28 -- MIME-Version: 1.0 7, 28 -- X-Received: by 10.140.108.9 with SMTP id i9mr59518512qgf.73.1420513339566; 7, 28 -- Mon, 05 Jan 2015 19:02:19 -0800 (PST) 7, 28 -- Received: by 10.96.118.1 with HTTP; Mon, 5 Jan 2015 19:02:19 -0800 (PST) 7, 28 -- Date: Mon, 5 Jan 2015 22:02:19 -0500 7, 28 -- Message-ID: {CAO94suncLfadFc2=b_TYFnr4Z+SZN0szUpB-Kqsr3AZKg_cpwA-at-mail.gmail.com} 7, 28 -- Subject: EM service contracts-Negotiating prices? 7, 28 -- From: Blanca Carbajal Gonzalez {bicarbaj-at-mtholyoke.edu} 7, 28 -- To: microscopy-at-microscopy.com 7, 28 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
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} realname - Ondrej Kotecky } Email - ondrej.kotecky-at-gmail.com } EDUCATION - Graduate College } LOCATION - Brno, Czech Republic } QUESTION - Hello, } } i had rubber sample made from two rubber components/compounds. The interface between the components was not visible in reflected light on a guillotine cut, and it was not visible on a polished section. (Polished similarly as a metallurgical sample. Polishing resulted in a nearly plane and slightly inclined surface.) Neither dark-field nor polarized-light could resolve the interface -- one colour across the whole sample. } } } From the above, i was assuming that there is no step in between the components (dark-field) and that the compounds give the same reflection in polarized light. So DIC should not show the interface. I tried anyway and was surprised to see two different colours on both sides of the interface. } } I'd like to understand why DIC shows a different colour. The literature did not help. I must be missing something. Could you help to explain why DIC results in two homogeneous regions with a distinct colour contrast? } } Many thanks, } Ondrej Kotecky
==============================Original Headers============================== 4, 33 -- From oshel1pe-at-cmich.edu Wed Jan 7 07:07:14 2015 4, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07D7CVf029518 4, 33 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 07:07:13 -0600 4, 33 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 4, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t07D79Sn019994 4, 33 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 08:07:10 -0500 4, 33 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 4, 33 -- cas1.central.cmich.local (2002:8dd1:f2b::8dd1:f2b) with Microsoft SMTP Server 4, 33 -- (TLS) id 14.3.195.1; Wed, 7 Jan 2015 08:07:09 -0500 4, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 33 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 4, 33 -- 14.3.195.1; Wed, 7 Jan 2015 08:07:08 -0500 4, 33 -- Message-ID: {54AD2F73.1080701-at-cmich.edu} 4, 33 -- Date: Wed, 7 Jan 2015 08:06:59 -0500 4, 33 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 4, 33 -- Reply-To: Ondrej Kotecky {ondrej.kotecky-at-gmail.com} 4, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 33 -- MIME-Version: 1.0 4, 33 -- To: micro {microscopy-at-microscopy.com} 4, 33 -- Subject: Re: Ask-A-Microscopist: DIC light microscopy of 2 rubber compounds 4, 33 -- in contact 4, 33 -- References: {1983698105.1257.1420620947588.JavaMail.EWHSERVER1324$-at-10.10.133.40} 4, 33 -- In-Reply-To: {1983698105.1257.1420620947588.JavaMail.EWHSERVER1324$-at-10.10.133.40} 4, 33 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 4, 33 -- Content-Transfer-Encoding: 7bit 4, 33 -- X-Originating-IP: [141.209.2.100] 4, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 33 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 33 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 4, 33 -- X-CanItPRO-Stream: default 4, 33 -- X-Canit-Stats-ID: 02NB17alG - ef731ed09bda - 20150107 4, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Email: guy.vermeildeconchard-at-fr.netgrs.com Name: Guy VERMEIL DE CONCHARD
Organization: BIOLOGIE SERVIER COMPANY
Title-Subject: [Filtered] genuine spare part JEM 100SX
Message: To be given 8 never used filaments type K (tungsten) Jeol part No. 804500070 - type MA113008(03) for Jeol transmission microscope type 100SX.
please, contact by mail.
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 7 07:55:44 2015 14, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07Dtibi017872 14, 17 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 07:55:44 -0600 14, 17 -- Message-ID: {54AD3AE0.2050205-at-microscopy.com} 14, 17 -- Date: Wed, 07 Jan 2015 07:55:44 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW: never used filaments genuine spare part JEM 100SX 14, 17 -- References: {201501070832.t078W4ln022210-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201501070832.t078W4ln022210-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201501070832.t078W4ln022210-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Organization: University of Massachusetts Medical School
Title-Subject: [Filtered] Recycling old EM negatives
Message: A colleague of mine found several boxes of old (from her dissertation) EM negatives while cleaning out her basement. She contacted me and asked if we could recycle them. It never crossed my mind that these could be recycled but apparently there are hundreds of them and it does seem kind of a waste to just through them out. Does anyone out there no of/or if Old EM negatives (Kodak 4489) can be recycled? Happy New year to you all Greg
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==============================Original Headers============================== 13, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 7 07:56:32 2015 13, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07DuWV8018798 13, 17 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 07:56:32 -0600 13, 17 -- Message-ID: {54AD3B10.3040609-at-microscopy.com} 13, 17 -- Date: Wed, 07 Jan 2015 07:56:32 -0600 13, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 13, 17 -- MIME-Version: 1.0 13, 17 -- To: microscopyListServer-Forward {microscopy-at-microscopy.com} 13, 17 -- Subject: viaWWW:Recycling old EM negatives 13, 17 -- References: {201501071333.t07DXCTi017215-at-ns.microscopy.com} 13, 17 -- In-Reply-To: {201501071333.t07DXCTi017215-at-ns.microscopy.com} 13, 17 -- X-Forwarded-Message-Id: {201501071333.t07DXCTi017215-at-ns.microscopy.com} 13, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
X-from a quick google, Kodak have a document called KES-60, which has a list of scrap film buyers, the most recent version I could find in Google was this:
On 07/01/2015 14:04, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
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==============================Original Headers============================== 10, 30 -- From ben.micklem-at-pharm.ox.ac.uk Wed Jan 7 08:13:02 2015 10, 30 -- Received: from relay15.mail.ox.ac.uk (relay15.mail.ox.ac.uk [163.1.2.163]) 10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07ED1jB024855 10, 30 -- for {Microscopy-at-Microscopy.com} ; Wed, 7 Jan 2015 08:13:02 -0600 10, 30 -- Received: from hub01.nexus.ox.ac.uk ([163.1.154.218] helo=HUB01.ad.oak.ox.ac.uk) 10, 30 -- by relay15.mail.ox.ac.uk with esmtp (Exim 4.80) 10, 30 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 10, 30 -- id 1Y8rM5-0005ye-n2 10, 30 -- for Microscopy-at-Microscopy.com; Wed, 07 Jan 2015 14:13:01 +0000 10, 30 -- Received: from MBX03.ad.oak.ox.ac.uk ([169.254.3.5]) by HUB01.ad.oak.ox.ac.uk 10, 30 -- ([163.1.154.92]) with mapi id 14.03.0169.001; Wed, 7 Jan 2015 14:13:00 +0000 10, 30 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 10, 30 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 10, 30 -- Subject: Re: [Microscopy] viaWWW:Recycling old EM negatives 10, 30 -- Thread-Topic: [Microscopy] viaWWW:Recycling old EM negatives 10, 30 -- Thread-Index: AQHQKoLPirOrbQ6QOkSUnvankMEcHpy0sw0A 10, 30 -- Date: Wed, 7 Jan 2015 14:13:00 +0000 10, 30 -- Message-ID: {D0D2EEF2.3F3B9%ben.micklem-at-pharm.ox.ac.uk} 10, 30 -- In-Reply-To: {201501071404.t07E48kb004689-at-ns.microscopy.com} 10, 30 -- Accept-Language: en-GB, en-US 10, 30 -- Content-Language: en-US 10, 30 -- X-MS-Has-Attach: 10, 30 -- X-MS-TNEF-Correlator: 10, 30 -- user-agent: Microsoft-MacOutlook/14.3.4.130416 10, 30 -- x-originating-ip: [172.16.150.240] 10, 30 -- Content-Type: text/plain; charset="utf-8" 10, 30 -- Content-ID: {85A27FDF5D9B4C4F95242DE7ED67ECE0-at-ad.oak.ox.ac.uk} 10, 30 -- MIME-Version: 1.0 10, 30 -- Content-Transfer-Encoding: 8bit 10, 30 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id t07ED1jB024855 ==============================End of - Headers==============================
Thank you very much for all the helpful advice and kind words. I learned about many alternatives for service that I will definitely be considering in the future.
I found out what the issue was with FEI. Apparently, we were being given an unusual 5% multi-tool discount which was reduced to 2% this year. However, we were not given notice of this reduction last year when we were preparing our budget (this happened before I started the position).
FEI was kind enough to negotiate to a 3.5% discount for this year, mostly because our budget was prepared with a 5% discount in mind.
More good news: I contacted our service engineer about the continued low vac problem. His response this time was prompt and he is coming out to inspect the instrument at the end of the week.
Before my post, I was not aware that the best plan of action was to voice my concerns to the service manager when I found myself dissatisfied with the service. Thank you for letting me know. Maybe now I won't have issues with obtaining prompt replies.
Thank you,
Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu
==============================Original Headers============================== 9, 28 -- From bicarbaj-at-mtholyoke.edu Wed Jan 7 11:45:33 2015 9, 28 -- Received: from mail-qg0-f50.google.com (mail-qg0-f50.google.com [209.85.192.50]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07HjXq2018025 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 11:45:33 -0600 9, 28 -- Received: by mail-qg0-f50.google.com with SMTP id z60so1176036qgd.37 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 07 Jan 2015 09:45:33 -0800 (PST) 9, 28 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 28 -- d=1e100.net; s=20130820; 9, 28 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to 9, 28 -- :content-type; 9, 28 -- bh=oiZ7Ele5Gm/qgS0h/zXTxDHcHhxlYYc3rghDW9Udld8=; 9, 28 -- b=QyP8hzqzOXbut6MyFu1e1gOO6SRnY0XB14KpwW4/dVU0OsbAPArbnJYVc9jx63Ql7K 9, 28 -- /nPU200c3t9jF9tViE6GhgrtQD75O00iHeZVApmmITMQPhIUqrtGjDo4c5qM7nNcv4EA 9, 28 -- 9VzX1+gp78VC1wzNAA+LXysIDxhINxA3D1N7W884CZf/Mnev/xqwBRIfnKWewLkyUPHm 9, 28 -- qrxfXr2gI7pdnPwq2yowMWaxqeJbFF7aWxrxjz1bxVXMBLJM+gz00EGcEiljyIh+bftT 9, 28 -- OQ+glOpFZxTFcDqvopihuvmwOUwzJf1oP0UZ3kR9UlxfiUB9/Z5CoDWJ8S8GMAgA23rK 9, 28 -- ak4g== 9, 28 -- X-Gm-Message-State: ALoCoQl8Qd6Jzkw6C0+QKU4RKXstbvk6ts2sRG7dZRtTtUaQ/UWhWYFp5R3rPRnqEsSen9PZ9onb 9, 28 -- MIME-Version: 1.0 9, 28 -- X-Received: by 10.224.43.3 with SMTP id u3mr6832680qae.50.1420652732929; Wed, 9, 28 -- 07 Jan 2015 09:45:32 -0800 (PST) 9, 28 -- Received: by 10.96.118.1 with HTTP; Wed, 7 Jan 2015 09:45:32 -0800 (PST) 9, 28 -- Date: Wed, 7 Jan 2015 12:45:32 -0500 9, 28 -- Message-ID: {CAO94su=N5J2iC_wocctoCpo2UCxY5ZPmBV=0JfoN+Fe7Pcaiig-at-mail.gmail.com} 9, 28 -- Subject: EM service contracts-Negotiating prices?--update 9, 28 -- From: Blanca Carbajal Gonzalez {bicarbaj-at-mtholyoke.edu} 9, 28 -- To: microscopy-at-microscopy.com 9, 28 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
There are people around the world that recovers silver from both unexposed film and negatives. Many have gathered together on the http://goldrefiningforum.com/ forum. There you could probably find someone close-by that can recover the silver from the films. The process is also described on several places on the forum for anyone interested.
/Göran
ben.micklem-at-pharm.ox.ac.uk skrev den 2015-01-07 15:13: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from a quick google, Kodak have a document called KES-60, which has a list } of scrap film buyers, the most recent version I could find in Google was } this: } } http://www.kodak.co.uk/ek/uploadedFiles/Content/About_Kodak/Global_Sustaina } bility/Health,_Safety_and_Environment/HSE_Support_Center/Product_End_of_Lif } e_Management/KES-60_Scrap_Film_Buyers.pdf } } } Ben } } } } On 07/01/2015 14:04, "microscopylistserver-noreply-at-microscopy.com" } {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } } } } -------------------------------------------------------------------------- } } -- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------------------- } } -- } } } } X-from: Gregory.Hendricks-at-umassmed.edu } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } -------------------------------------------------------------------------- } } - } } Remember this posting is most likely not from a Subscriber, so when } } replying } } please copy both Gregory.Hendricks-at-umassmed.edu as well as the } } Microscopy Listserver } } -------------------------------------------------------------------------- } } - } } } } Email: Gregory.Hendricks-at-umassmed.edu } } Name: Greg Hendricks } } } } Organization: University of Massachusetts Medical School } } } } Title-Subject: [Filtered] Recycling old EM negatives } } } } Message: A colleague of mine found several boxes of old (from her } } dissertation) EM negatives while } } cleaning out her basement. She contacted me and asked if we could } } recycle them. It never crossed } } my mind that these could be recycled but apparently there are hundreds of } } them and it does seem kind } } of a waste to just through them out. Does anyone out there no of/or if } } Old EM negatives (Kodak } } 4489) can be recycled? } } Happy New year to you all } } Greg } } } } Login Host: 146.189.245.29 } } Listserver Email Form V - 20120416 } } -------------------------------------------------------------------------- } } - } } } } } } } } } } -- } } =========================================== } } Do not reply to this message it is from } } the Microscopy Listserver NO-REPLY forwarding } } system. 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==============================Original Headers============================== 4, 24 -- From axelsson-at-acc.umu.se Wed Jan 7 17:34:03 2015 4, 24 -- Received: from mail.acc.umu.se (mail.acc.umu.se [130.239.18.156]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t07NY2JX016771 4, 24 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2015 17:34:03 -0600 4, 24 -- Received: from localhost (localhost [127.0.0.1]) 4, 24 -- by amavisd-new (Postfix) with ESMTP id 909EBB40 4, 24 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 00:34:02 +0100 (MET) 4, 24 -- X-Virus-Scanned: amavisd-new at acc.umu.se 4, 24 -- Received: from [192.168.1.140] (dynamic.1.23.c4acbfe70.0405c18da6.cust.bredband2.com [89.233.195.134]) 4, 24 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 4, 24 -- (No client certificate requested) 4, 24 -- by mail.acc.umu.se (Postfix) with ESMTPS id E67B2B3F 4, 24 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 00:34:00 +0100 (MET) 4, 24 -- Message-ID: {54ADC263.3050900-at-acc.umu.se} 4, 24 -- Date: Thu, 08 Jan 2015 00:33:55 +0100 4, 24 -- From: =?windows-1252?Q?G=F6ran_Axelsson?= {axelsson-at-acc.umu.se} 4, 24 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 4, 24 -- MIME-Version: 1.0 4, 24 -- To: microscopy-at-microscopy.com 4, 24 -- Subject: Re: [Microscopy] Re: viaWWW:Recycling old EM negatives 4, 24 -- References: {201501071413.t07ED73e024909-at-ns.microscopy.com} 4, 24 -- In-Reply-To: {201501071413.t07ED73e024909-at-ns.microscopy.com} 4, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 24 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: leex3870-at-umn.edu Name: Han
Organization: University of Minnesota
Title-Subject: [Filtered] Compresser problem in RMC RFD-9010CR freeze fracture machine
Message: Hello
I have a RMC RFD-9010CR freeze fracture machine and its compressor (Mitsubishi super line SP-KR) is not working. I checked fuses in the mainboard, and none of them is blown. I'm wondering that anyone had similar problem with RMC freeze fracture machine can give some advice so that I can start with. Thanks, Han
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==============================Original Headers============================== 12, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 8 06:54:37 2015 12, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t08Csbfd013530 12, 17 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 06:54:37 -0600 12, 17 -- Message-ID: {54AE7E0D.5010402-at-microscopy.com} 12, 17 -- Date: Thu, 08 Jan 2015 06:54:37 -0600 12, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 12, 17 -- MIME-Version: 1.0 12, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 17 -- Subject: viaWWW:Compresser problem in RMC RFD-9010CR freeze fracture machine 12, 17 -- References: {201501072320.t07NKinB016338-at-ns.microscopy.com} 12, 17 -- In-Reply-To: {201501072320.t07NKinB016338-at-ns.microscopy.com} 12, 17 -- X-Forwarded-Message-Id: {201501072320.t07NKinB016338-at-ns.microscopy.com} 12, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: afmhelp-at-gmail.com Name: Peter Eaton
Organization: UCIBIO-at-Requimte / University of Porto, Portugal
Title-Subject: [Filtered] AFM Training course 2015
Message: Dear All, The 4th Porto Atomic Force Microscopy Training Workshop will run during Easter 2015, from the 30th March to 2nd April in the historic city of Porto, Portugal. Following the successful courses that ran in 2011, 2013 and 2014, the course will include several hours hands-on training in acquiring images with the atomic force microscope as well as AFM data processing. Other topics covered in lectures include AFM modes, AFM instrumentation, sample preparation, and applications. The course will also feature advanced topics lectures from guest scientists in biology and materials science. Those interested in attending are encouraged to register as soon as possible, as the course usually fills quickly. More details can be found at http://afmhelp.com/course, and enquiries can be sent to afmhelp-at-gmail.com. Regards, Dr. Peter Eaton
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 8 06:55:39 2015 14, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t08CtdEW014194 14, 17 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 06:55:39 -0600 14, 17 -- Message-ID: {54AE7E4B.4020500-at-microscopy.com} 14, 17 -- Date: Thu, 08 Jan 2015 06:55:39 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:AFM Training course 2015 - Portugal 14, 17 -- References: {201501081019.t08AJ3Mf009403-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201501081019.t08AJ3Mf009403-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201501081019.t08AJ3Mf009403-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You may recall that I asked the list about what to include in a bachelor's level EM curriculum.
I received many responses, mostly directly to me, and a few that were shared on the list.
I am still working on this project and want to thank everyone who has contributed. Keep those cards and letters coming, more ideas are always better.
I got several requests to post the replies to the list, but the page count is now up to 11 and I think that's too long to post. So, if you would like to receive a copy of the replies, send me a request and I will send a PDF to you. Fear not, I think I have sanitized the responses so you have nothing to fear from the thought police. If you sent a reply and do not wish to have it shared, let me know and I will take care of it.
Jon
-- Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Avenue Stockton, CA 95207 jkrupp-at-deltacollege.edu (209) 954-5284
Find us on Facebook at Electron Microscopy at SJ Delta College
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Email: czyrska-at-agh.edu.pl Name: Aleksandra Czyrska-Filemonowicz
Organization: Centre of Electron Microscopy for Materials Science, AGH University of Science and Technology, Krakow, Poland
Title-Subject: [Filtered] Post-doctorate or senior electron microscopist
Message: The Faculty of Metals Engineering and Industrial Computer Sciences and International Centre of Electron Microscopy for Materials Science (IC-EM) invite applications for an experienced Post-Doctorate Scientist in materials science and/or physics to strengthen and further develop an eleven people team.
The present research frame is focused on quantitative characterisation of micro/nanostructure and properties of various materials, structure-property relationship as well as tailoring microstructure for desired properties. Transmission electron microscopy (TEM) is the main investigation tool around a probe Cs corrected Titan Cubed 60-300 with a ChemiSTEM, Merlin Gemini II SEM and comprehensive preparation laboratory with FIB and NanoMill 1040. More details are given on the Centre website: http://www.tem.agh.edu.pl
This offer aims at a person with a PhD degree in materials science or physics. Proven ability to conduct academic research (mainly using TEM), ability to make clear oral and written presentations as well as self-motivation are prerequisite. Ability to write publications in international journals, fluent English (spoken and written) are expected. Teaching ability in English and in Polish is required. Female scientists are encouraged to apply.
The successful candidate will take the responsibility for research within existing projects and is expected to develop her/his own projects on a longer perspective based on national, international and industry funding.
The call is open immediately and will be closed on 10th of February, 2015. The University position will be open from 1st of March 2015 and secure for the next two years with a possibility of further prolongation.
Please send your application (CV and publications list) together with the names of two referees (phone, e-mail and website addresses) by e-mail to:
Prof. Dr. Aleksandra Czyrska-Filemonowicz AGH University of Science and Technology, Krakow, Poland Tel. +48 12 617 2929 or +48 12 617 2587 (secretary) email: czyrska-at-agh.edu.pl http://www.tem.agh.edu.pl
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==============================Original Headers============================== 25, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 8 18:19:10 2015 25, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 25, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t090JAsS002956 25, 17 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 18:19:10 -0600 25, 17 -- Message-ID: {54AF1E7E.8080701-at-microscopy.com} 25, 17 -- Date: Thu, 08 Jan 2015 18:19:10 -0600 25, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 25, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 25, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 25, 17 -- MIME-Version: 1.0 25, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 25, 17 -- Subject: viaWWW:Post-doctorate or senior electron microscopist / Krakow, Poland 25, 17 -- References: {201501081527.t08FRFu3027322-at-ns.microscopy.com} 25, 17 -- In-Reply-To: {201501081527.t08FRFu3027322-at-ns.microscopy.com} 25, 17 -- X-Forwarded-Message-Id: {201501081527.t08FRFu3027322-at-ns.microscopy.com} 25, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 25, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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I'm looking for input on preparing some very testy powder samples of mesoporous silica embedded with small metal nanoparticles. The goal is to get cross-sectional HRTEM to show that the particles are embedded into the walls of the silica, instead in the pores/surface. Imaging the material as powder doesn't show any of the tubular bundles in the correct orientation (looking down the pores), so I've tried cross sections.
FIB chewed up the material, so the next approach was to embed in epoxy and microtome slices. My collaborator used a general procedure from the technical guide, 19:21:1.2 Araldite502:DDSA:BDMA with powder mixed in, at 60C for 24h after degassing, in BEEM capsules. The epoxy slices (70-200nm) were relatively soft, had a couple bubbles, and often split lengthwise during cutting. The admin of our facility suggested that the polymerizarion was unfinished. Under our FEI Titan beam at both 80kV and 300kV, the slices crumpled & broke immediately at medium magnification (3800X) with a spread beam (spot size 4), and including after exposure/pre-warming at low mag, which was ok.
Assuming that microtoming is the way to go, can anyone suggest modifications to the embedding procedure to make the slices more robust? Or TEM tricks to prevent breaking? Or, what techniques might be able to cross section powder better? The silica pores/channels (~10nm diameter) also present a challenge because they swell under the beam, and being embedded makes the ~5nm nps harder to find...
Thanks to anyone who read all that! Those of us on this projects are new at the microtome, so all input is very appreciated.
-Chilan
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==============================Original Headers============================== 18, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 8 18:20:13 2015 18, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 18, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t090KDep003744 18, 17 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 18:20:13 -0600 18, 17 -- Message-ID: {54AF1EBD.4020802-at-microscopy.com} 18, 17 -- Date: Thu, 08 Jan 2015 18:20:13 -0600 18, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 18, 17 -- MIME-Version: 1.0 18, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 17 -- Subject: viaWWW:Cross-section Preparation of mesoporous silica 18, 17 -- References: {201501081858.t08IwhZo018915-at-ns.microscopy.com} 18, 17 -- In-Reply-To: {201501081858.t08IwhZo018915-at-ns.microscopy.com} 18, 17 -- X-Forwarded-Message-Id: {201501081858.t08IwhZo018915-at-ns.microscopy.com} 18, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
If you asked for a PDF of the replies I got to my question about EM curriculum, sit tight.
The file is on my other computer and I won't get to it for about a week. Will send out when I can.
Jon
-- Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Avenue Stockton, CA 95207 jkrupp-at-deltacollege.edu (209) 954-5284
Find us on Facebook at Electron Microscopy at SJ Delta College
I've looked at many specimens of mesoporous silica embedded with nanoparticles in my time, as well as a fair few microtomed specimens - and have a few suggestions;
1) Stop using TEM - use STEM instead, preferably some flavour of ADF imaging as the contrast from the metal nanoparticles will be strong, and there will be no phase contrast making interpretation harder.
Also both mesoporous silica and the embedding polymer undergo damage via electron beam heating and/or electrostatic charging induced tearing. As the total dose rate is much lower in STEM (and a moving probe means no area is exposed for long) damage will be much less of a problem. Also note this means high voltage means LESS damage for the silica - as the inelastic cross section drops with increasing voltage. As such 300kV is orders of magnitude more stable than 80kV for these materials.
2) Coat your sections with a monolayer of carbon after microtoming. As heating/charging is the damage mechanism this will provide a conduction path for removing charge and heat. (In some microtomed sections this converts specimens from being unusable in STEM to perfectly stable).
3) To make it easier to see end on pores in the powder you should grind the sample before dispersing it in alcohol - and sonicate it well. The raw material tends to form in micelles that are 'sausage' shaped with the pores along the long axis of the sausage. This is what makes it unlikely to come across end on pores in an unground specimen.
4) Tilt! If you cant see end on pores when flat your microscope has a double tilt stage - use it. Even better carry out tomography as you'll be able to resolve internal/external and particle positions in the reconstruction. Note that whatever your specimen prep technique only the tilting approaches will allow you to say with any certainty where your nanoparticles are in relation to the silica - all other imaging techniques will be limited by projection.
Hope that helps,
Matthew
On 9/01/2015 11:39 AM, microscopylistserver-noreply-at-microscopy.com wrote:
} Organization: UCLA Materials Science } } Title-Subject: [Filtered] Cross-section Preparation } } Message: Dear all, } } I'm looking for input on preparing some very testy powder samples of } mesoporous silica embedded with small metal nanoparticles. The goal } is to get cross-sectional HRTEM to show that the particles are } embedded into the walls of the silica, instead in the pores/surface. } Imaging the material as powder doesn't show any of the tubular } bundles in the correct orientation (looking down the pores), so I've } tried cross sections. } } FIB chewed up the material, so the next approach was to embed in } epoxy and microtome slices. My collaborator used a general procedure } from the technical guide, 19:21:1.2 Araldite502:DDSA:BDMA with powder } mixed in, at 60C for 24h after degassing, in BEEM capsules. The epoxy } slices (70-200nm) were relatively soft, had a couple bubbles, and } often split lengthwise during cutting. The admin of our facility } suggested that the polymerizarion was unfinished. Under our FEI Titan } beam at both 80kV and 300kV, the slices crumpled & broke immediately } at medium magnification (3800X) with a spread beam (spot size 4), and } including after exposure/pre-warming at low mag, which was ok. } } Assuming that microtoming is the way to go, can anyone suggest } modifications to the embedding procedure to make the slices more } robust? Or TEM tricks to prevent breaking? Or, what techniques might } be able to cross section powder better? The silica pores/channels } (~10nm diameter) also present a challenge because they swell under } the beam, and being embedded makes the ~5nm nps harder to find... } } Thanks to anyone who read all that! Those of us on this projects are } new at the microtome, so all input is very appreciated. } } -Chilan
-- Dr M.Weyland, Associate Professor & Titan Manager -------------------------------------------------------------------------- Monash Centre for Electron Microscopy Bldg 81 Monash University Victoria 3800 Australia. -------------------------------------------------------------------------- www.mcem.monash.edu.au ------------------------------------------------------------------------- Ph: (+61) 3 990 59026 --- Fax: (+61) 3 990 53600 --------------------------------------------------------------------------
==============================Original Headers============================== 13, 41 -- From matthew.weyland-at-monash.edu Thu Jan 8 23:03:06 2015 13, 41 -- Received: from na3sys009aog124.obsmtp.com (na3sys009aog124.obsmtp.com [74.125.149.151]) 13, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t09535V2008403 13, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Jan 2015 23:03:05 -0600 13, 41 -- Received: from mail-pd0-f172.google.com ([209.85.192.172]) (using TLSv1) by na3sys009aob124.postini.com ([74.125.148.12]) with SMTP 13, 41 -- ID DSNKVK9hCEGWqGRuEgORF/rrdAYXLncB/XOB-at-postini.com; Thu, 08 Jan 2015 21:03:06 PST 13, 41 -- Received: by mail-pd0-f172.google.com with SMTP id y13so15335057pdi.3 13, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 08 Jan 2015 21:03:04 -0800 (PST) 13, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 41 -- d=1e100.net; s=20130820; 13, 41 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 13, 41 -- :cc:subject:references:in-reply-to:content-type 13, 41 -- :content-transfer-encoding; 13, 41 -- bh=ebziDJocUcY6TywWTdDslsV8/iSZ83iZN3o2G4WEEZM=; 13, 41 -- b=Sc62iB/zdFK8uRooX72/Z16uH9leu5KWOToag/K9U2ZTp0rQmUbEpeAOAgV1hJkqk2 13, 41 -- MftBtGTLS77z1s/Bz0PHO5KYcIjgfw8MCokBZ75IYaD2UaBt5Kuuuw/T4c6u1cIzNIV1 13, 41 -- ifDHwFRSpTFfiZmc3A12rmlp+yoWrpEVYiyS2ofG4qZ5hwbLh9T4y/mSc21gOkO0SeZL 13, 41 -- 3ndvv+/X/KTA059tR8ne9pkWPqivycyka5XGlXTJoPPgEW1y+sm8+cTCMPIpUqZ7Hmp3 13, 41 -- 0zKW4ELk+W6Lap9c59g1YpjdWe/JxvE8XnLlxexFBW8vE62iNsfOoh5O5aVkJzt5A50g 13, 41 -- Py2Q== 13, 41 -- X-Gm-Message-State: ALoCoQlXneIBL7JEHomgcoortAz0GsvyVR32b5OA72EhF8P1S0UjnG/W95FBi6mLFwNVSsZbLkSnn6hAliJWAiGdW4vZ2Hl4vU6tXBeLWDf7425JOBEYkQEwONWZjGTPVH7fzp4dhMvnPrpQOKb06AzrbZuRrdzkhw== 13, 41 -- X-Received: by 10.70.138.37 with SMTP id qn5mr21011936pdb.118.1420779784129; 13, 41 -- Thu, 08 Jan 2015 21:03:04 -0800 (PST) 13, 41 -- X-Received: by 10.70.138.37 with SMTP id qn5mr21011914pdb.118.1420779783964; 13, 41 -- Thu, 08 Jan 2015 21:03:03 -0800 (PST) 13, 41 -- Received: from [192.168.1.10] (ppp118-209-72-8.lns20.mel4.internode.on.net. [118.209.72.8]) 13, 41 -- by mx.google.com with ESMTPSA id xl12sm5889037pac.41.2015.01.08.21.03.01 13, 41 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 41 -- Thu, 08 Jan 2015 21:03:03 -0800 (PST) 13, 41 -- Message-ID: {54AF60F6.6060807-at-monash.edu} 13, 41 -- Date: Fri, 09 Jan 2015 16:02:46 +1100 13, 41 -- From: Matthew Weyland {matthew.weyland-at-monash.edu} 13, 41 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 13, 41 -- MIME-Version: 1.0 13, 41 -- To: Microscopy-at-microscopy.com 13, 41 -- CC: chilan.ngo-at-ucla.edu 13, 41 -- Subject: Re: viaWWW:Cross-section Preparation of mesoporous silica 13, 41 -- References: {201501090039.t090dA9c006083-at-ns.microscopy.com} 13, 41 -- In-Reply-To: {201501090039.t090dA9c006083-at-ns.microscopy.com} 13, 41 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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ealname - Francoise Marga Email - fmarga-at-modernmeadow.com ORGANIZATION - Modern Meadow EDUCATION - Graduate College LOCATION - Brooklyn, NY, USA SUBJECT_OF_QUESTION - SEM in NYC QUESTION - Hi,
Our company would like to look at our samples by SEM. We need to go up x15,000 to visualize collagen fibers. As a business, we have trouble to find a SEM accessible to a private company. Does anyone know a facility or a private service in our area (Brooklyn, NY) that could help us. Thanks for your help. Kind regards,
Francoise
==============================Original Headers============================== 6, 31 -- From oshel1pe-at-cmich.edu Mon Jan 12 15:53:10 2015 6, 31 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0CLrAld023845 6, 31 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2015 15:53:10 -0600 6, 31 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 6, 31 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0CLrAee031293 6, 31 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2015 16:53:10 -0500 6, 31 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 6, 31 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 6, 31 -- (TLS) id 14.3.195.1; Mon, 12 Jan 2015 16:53:04 -0500 6, 31 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 6, 31 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 6, 31 -- 14.3.195.1; Mon, 12 Jan 2015 16:53:05 -0500 6, 31 -- Message-ID: {54B44244.2030207-at-cmich.edu} 6, 31 -- Date: Mon, 12 Jan 2015 16:53:08 -0500 6, 31 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 6, 31 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 31 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 31 -- MIME-Version: 1.0 6, 31 -- To: micro {microscopy-at-microscopy.com} 6, 31 -- Subject: Ask-A-Microscopist: SEM service available in Brooklyn NY area for 6, 31 -- collagen fibers? 6, 31 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 31 -- Content-Transfer-Encoding: 7bit 6, 31 -- X-Originating-IP: [141.209.2.100] 6, 31 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 31 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 6, 31 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 6, 31 -- X-CanItPRO-Stream: default 6, 31 -- X-Canit-Stats-ID: 02ND9Rax7 - 0460a495d384 - 20150112 6, 31 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Microscope Society of NE Ohio (MSNO)
Title-Subject: [Filtered] MSNO 2015 meeting
Message: MSNO 2015 Winter Meeting
MSNO 2015 Winter Meeting - Wednesday, March 4th, 2015, 3:00 – 8:30 p.m. at Cleveland Museum of Natural History (1 Wade Oval Drive, University Circle, Cleveland, OH 44106)
Lillian A. Kuri from Cleveland Foundation will give a lecture on "ClevelandÂ’s Greater University Circle Initiative" and John Hemsath - Retired Director of Theater Operations will give a lecture on "The History of Playhouse Square"
Registration including dinner will be $20 for MSNO members, $25 for non-members and $5 for student members, $10 for student non-members. Preregistration is available at http://www.msneo.org/meetings.html, or registration and payment at the door will also be available (additional 5$ for all). Preregistration is required so we can get a good head count.
Please see more detail at https://www.facebook.com/MicroscopySociety/photos/pcb.631540963642067/631540420308788/?type=1&theater
Please contact nxa157-at-case.edu for more information.
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==============================Original Headers============================== 21, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 13 19:27:08 2015 21, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 21, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0E1R8Vr007935 21, 17 -- for {microscopy-at-microscopy.com} ; Tue, 13 Jan 2015 19:27:08 -0600 21, 17 -- Message-ID: {54B5C5EC.2020506-at-microscopy.com} 21, 17 -- Date: Tue, 13 Jan 2015 19:27:08 -0600 21, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 21, 17 -- MIME-Version: 1.0 21, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 17 -- Subject: viaWWW:MSNO 2015 meeting 21, 17 -- References: {201501132040.t0DKeu7t003559-at-ns.microscopy.com} 21, 17 -- In-Reply-To: {201501132040.t0DKeu7t003559-at-ns.microscopy.com} 21, 17 -- X-Forwarded-Message-Id: {201501132040.t0DKeu7t003559-at-ns.microscopy.com} 21, 17 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] Research Fellow in Scanning Transmission Electron Microscopy Imaging and Simulations of Interfaces in Light Alloys
Message: The following position is currently available:
Research Fellow in Scanning Transmission Electron Microscopy Imaging and Simulations of Interfaces in Light Alloys
Department of Materials Engineering, Monash University, Australia
Application closing date: 15 February 2015
http://jobs.monash.edu.au/jobDetails.asp?sJobIDs=530367 or http://users.monash.edu/~bourgeoi/Open-Positions.html
A/Prof. Laure Bourgeois Associate Professor - Microscope Manager Monash Centre for Electron Microscopy -- Department of Materials Engineering Building 81, 10 Innovation Walk Monash University, VIC 3800, Australia Tel: +61-(0)3-9905-5368 -- Fax: +61-(0)3-9905-3600 www.mcem.monash.edu.au users.monash.edu/~bourgeoi
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==============================Original Headers============================== 23, 18 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 14 06:56:33 2015 23, 18 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 23, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0ECuW5c012967 23, 18 -- for {microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 06:56:33 -0600 23, 18 -- Message-ID: {54B66780.9040002-at-microscopy.com} 23, 18 -- Date: Wed, 14 Jan 2015 06:56:32 -0600 23, 18 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 18 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 23, 18 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 23, 18 -- MIME-Version: 1.0 23, 18 -- To: microscopyListServer-Forward {microscopy-at-microscopy.com} 23, 18 -- Subject: viaWWW:Research Fellow in Scanning Transmission Electron Microscopy 23, 18 -- Imaging and Simulations of Interfaces in Light Alloys 23, 18 -- References: {201501140517.t0E5Hpqe000633-at-ns.microscopy.com} 23, 18 -- In-Reply-To: {201501140517.t0E5Hpqe000633-at-ns.microscopy.com} 23, 18 -- X-Forwarded-Message-Id: {201501140517.t0E5Hpqe000633-at-ns.microscopy.com} 23, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 23, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: grottigni-at-areialab.com Name: Rottigni V. Guglielmo
Organization: AREIA LABORATOIRES -NORMANDY - FRANCE
I use a Cressington 108 Carbon Coater to make carbon strata on polycarbonate filters, following ISO 13794 (the filters, after coating, are used to filter suspensions containing asbestos fibers), but _ while mantaining the same conditions (usually 4.5 V qnd three shots of " seconds each), the quantity of carbon deposed vary very much, and randomly. Sometimes I obtain 20 nm, sometimes only 10. Is there someone using the same Cressington Coater who may give me some advice qbout the best way to obtain carbon films of constant thickness?
Thanks in advance
Guglielmo Rottigni AREIA LABORATOIRES www.areialab.com Bourgtheroulde-Infreville Normandy FRANCE
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==============================Original Headers============================== 17, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 14 06:57:13 2015 17, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 17, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0ECvDS8013604 17, 17 -- for {microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 06:57:13 -0600 17, 17 -- Message-ID: {54B667A9.6030105-at-microscopy.com} 17, 17 -- Date: Wed, 14 Jan 2015 06:57:13 -0600 17, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 17, 17 -- MIME-Version: 1.0 17, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 17 -- Subject: viaWWW:CRESSINGTON 108 CARBON COATER 17, 17 -- References: {201501141113.t0EBDl6J007676-at-ns.microscopy.com} 17, 17 -- In-Reply-To: {201501141113.t0EBDl6J007676-at-ns.microscopy.com} 17, 17 -- X-Forwarded-Message-Id: {201501141113.t0EBDl6J007676-at-ns.microscopy.com} 17, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Electron Microscopy Facility of the Campus Science Support Facilities (Vienna, Austria) and NexÂpeÂrion - Solutions for Electron Microscopy (Vienna, Austria) are jointly organising an international
Advanced Course on Cryo-Electron Tomography Vienna, Austria, Europe June 6–7, 2015: Optional Pre-Course June 8–12, 2015: Main Course
for students, post-doctoral staff, scientists, and group leaders from academia and industry. While this course targets an advanced audience pre-existing knowledge in electron tomography, data processing and/or Cryo-TEM), a weekend pre-course will be offered for less experienced participants to catch-up.
The main part of the course will cover
Immersion freezing for cryo-electron tomography Cryo-CLEM Low-dose data collection with SeriÂalEM Processing of low-dose tilt series with IMOD Modelling and interpretation of cryo-electron tomography data SubtoÂmoÂgram averaging with PEET
Instructors and Organisers
Dr. Thomas Heuser, Campus Science Support Facilities, Vienna, Austria Dr. Johanna Höög, University of Gothenburg, Sweden Dr. David MasÂtronarde, University of Colorado, Boulder, United States Dr. ReinÂhard Rachel, University of Regensburg, Germany Dr. Guenter Resch, NexÂpeÂrion e.U. - Solutions for Electron Microscopy, Vienna, Austria
Sponsored Guest Lectures
Dr. Andreas KasÂtenÂmüller, Gatan GmbH, Munich, Germany Dr. Ruwin Pandithage, Leica Microsystems, Vienna, Austria
For details about on-site infrastructure, the registration procedure and fees, see
http://www.nexperion.net/cryotomo2015
We are looking forward to seeing you there, best regards,
Guenter on behalf of all organizers
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
==============================Original Headers============================== 7, 25 -- From lists-at-nexperion.net Wed Jan 14 11:15:25 2015 7, 25 -- Received: from 503.hosttech.eu (503.hosttech.eu [62.112.145.5]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0EHFPQX027330 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 11:15:25 -0600 7, 25 -- Received: from localhost (unknown [127.0.0.1]) 7, 25 -- by 503.hosttech.eu (Postfix) with ESMTP id ABEE66CA0D1 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 17:15:31 +0000 (UTC) 7, 25 -- X-Virus-Scanned: amavisd-new at example.com 7, 25 -- Received: from 503.hosttech.eu ([127.0.0.1]) 7, 25 -- by localhost (503.hosttech.eu [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id Lyccsxea2nV6 for {Microscopy-at-microscopy.com} ; 7, 25 -- Wed, 14 Jan 2015 18:15:16 +0100 (CET) 7, 25 -- Received: from aventine (chello062178180023.17.14.vie.surfer.at [62.178.180.23]) 7, 25 -- by 503.hosttech.eu (Postfix) with ESMTPSA id 645226CA0CD 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2015 18:15:16 +0100 (CET) 7, 25 -- Date: Wed, 14 Jan 2015 18:15:07 +0100 7, 25 -- From: Guenter Resch {lists-at-nexperion.net} 7, 25 -- To: Microscopy-at-microscopy.com 7, 25 -- Subject: Advanced Course on Cryo-Electron Tomography, Vienna, June 2015 7, 25 -- Message-ID: {20150114181507.7de1b05d-at-aventine} 7, 25 -- X-Mailer: Claws Mail 3.9.3 (GTK+ 2.24.23; x86_64-pc-linux-gnu) 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=UTF-8 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0EHFPQX027330 ==============================End of - Headers==============================
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Email: javaidqazi-at-kemet.com Name: Javaid Qazi
Organization: Kemet Electronics
Title-Subject: [Filtered] Feed back on SEM-EDS systems
Message: All,
I am planning to buy a SEM with EDS. I would like to get feedback from the group.
I am interested in knowing pros and cons between the two Hitachi SU3500 and JEOL IT300LV to decide which one to get.
In terms of EDS which systems are better, any recommendation both in terms of detector size and software usage.
The equipment will typically be used by 3-4 users who do have experience using the SEM-EDS.
I will take the feedback offline.
Thanks for your help.
Javaid
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I just ordered the same OSRAM 103 W/2 bulbs I've been using for years in a Zeiss HBO 100 unit. The cathode (top) of all 3 bulbs that arrived is too wide to fit into the HBO 100's clamp (bottom cathode is OK). Comparing with the old bulb I ordered 9 months ago, the anode is indeed wider now than before. Has anyone else received OSRAM 103 W/2 bulbs recently and were the dimensions OK? I got mine from Bulbtronics.com. How about with the similar USHIO 103D?
Thanks, Esteban
==============================Original Headers============================== 3, 27 -- From g.esteban.fernandez-at-gmail.com Fri Jan 16 00:50:12 2015 3, 27 -- Received: from mail-yh0-f51.google.com (mail-yh0-f51.google.com [209.85.213.51]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0G6oCVi015337 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2015 00:50:12 -0600 3, 27 -- Received: by mail-yh0-f51.google.com with SMTP id a41so9377388yho.10 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=20120113; 3, 27 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 27 -- bh=SyoELpsT7M2u4ScUjeNrLiZTvRKY3Z3nYrn4nN+BzFQ=; 3, 27 -- b=XSWNgtdH9aMRBoVL0o7jHomBH2jJBMZsoBizofA7LtITLCx4PAKNW+3VVj50XgYMuJ 3, 27 -- 4MzA8r+kjFSnt9jRt77lahEtck8uIsSi7xUIovwgukBDndF4g1HOC2n7Y5FgPQ3Ay1L5 3, 27 -- S1gjU+m84I50zkdLvv+GbI16G0bU/zmCd+uOL19STlE8rPIVOwVyCzCmdOfVn1RSRm6i 3, 27 -- xFn1NVNHqPkhw/C+JAyjvfC3lStfcHa2uUV2vt2swrvDGDszFEJKyNyUR7Q49B3orfI2 3, 27 -- 6b80ndL49mB8LT76GicMVhTUTvqUxkEMmSAG8cfBEsIurV+wJt9v8q6Kc5d2GHAfgPFX 3, 27 -- dTYQ== 3, 27 -- MIME-Version: 1.0 3, 27 -- X-Received: by 10.170.61.202 with SMTP id d193mr9936210ykd.32.1421391010895; 3, 27 -- Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- Received: by 10.170.174.134 with HTTP; Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- Date: Thu, 15 Jan 2015 22:50:10 -0800 3, 27 -- Message-ID: {CAB_3w78mthi57-Kc4L_dx=pbkoRhmjZYqodbpm7RLLQ6aXMEuw-at-mail.gmail.com} 3, 27 -- Subject: Changed dimensions of OSRAM mercury bulb 103 W/2? 3, 27 -- From: "G. Esteban Fernandez" {g.esteban.fernandez-at-gmail.com} 3, 27 -- To: Confocal Microscopy List {CONFOCALMICROSCOPY-at-lists.umn.edu} , 3, 27 -- microscopy-at-microscopy.com 3, 27 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Does anyone have a TEM digital imaging plate system - the Ditabis system, any others? - sitting around no longer being used? And looking to get rid of it?
A couple have come up on the list over the years, as we all move to digital cameras, but have a unique imaging experiment system we've been playing around with and these plates might serve really well.
No need to clog the list so just contact me offline.
Thank you!
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
The specifications of the bulb including dimensions of the anode are given at this link file:///C:/Users/phillipst/Downloads/ZMP_56327.pdf - it would be interesting to see whether the match your old bulb or the new one. Let us know the result since I also use those bulbs.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: g.esteban.fernandez-at-gmail.com [mailto:g.esteban.fernandez-at-gmail.com] Sent: Friday, January 16, 2015 12:51 AM To: Phillips, Thomas E.
Hi everyone,
I just ordered the same OSRAM 103 W/2 bulbs I've been using for years in a Zeiss HBO 100 unit. The cathode (top) of all 3 bulbs that arrived is too wide to fit into the HBO 100's clamp (bottom cathode is OK). Comparing with the old bulb I ordered 9 months ago, the anode is indeed wider now than before. Has anyone else received OSRAM 103 W/2 bulbs recently and were the dimensions OK? I got mine from Bulbtronics.com. How about with the similar USHIO 103D?
Thanks, Esteban
==============================Original Headers============================== 3, 27 -- From g.esteban.fernandez-at-gmail.com Fri Jan 16 00:50:12 2015 3, 27 -- Received: from mail-yh0-f51.google.com (mail-yh0-f51.google.com [209.85.213.51]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0G6oCVi015337 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2015 00:50:12 -0600 3, 27 -- Received: by mail-yh0-f51.google.com with SMTP id a41so9377388yho.10 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=20120113; 3, 27 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 27 -- bh=SyoELpsT7M2u4ScUjeNrLiZTvRKY3Z3nYrn4nN+BzFQ=; 3, 27 -- b=XSWNgtdH9aMRBoVL0o7jHomBH2jJBMZsoBizofA7LtITLCx4PAKNW+3VVj50XgYMuJ 3, 27 -- 4MzA8r+kjFSnt9jRt77lahEtck8uIsSi7xUIovwgukBDndF4g1HOC2n7Y5FgPQ3Ay1L5 3, 27 -- S1gjU+m84I50zkdLvv+GbI16G0bU/zmCd+uOL19STlE8rPIVOwVyCzCmdOfVn1RSRm6i 3, 27 -- xFn1NVNHqPkhw/C+JAyjvfC3lStfcHa2uUV2vt2swrvDGDszFEJKyNyUR7Q49B3orfI2 3, 27 -- 6b80ndL49mB8LT76GicMVhTUTvqUxkEMmSAG8cfBEsIurV+wJt9v8q6Kc5d2GHAfgPFX 3, 27 -- dTYQ== 3, 27 -- MIME-Version: 1.0 3, 27 -- X-Received: by 10.170.61.202 with SMTP id d193mr9936210ykd.32.1421391010895; 3, 27 -- Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- Received: by 10.170.174.134 with HTTP; Thu, 15 Jan 2015 22:50:10 -0800 (PST) 3, 27 -- Date: Thu, 15 Jan 2015 22:50:10 -0800 3, 27 -- Message-ID: {CAB_3w78mthi57-Kc4L_dx=pbkoRhmjZYqodbpm7RLLQ6aXMEuw-at-mail.gmail.com} 3, 27 -- Subject: Changed dimensions of OSRAM mercury bulb 103 W/2? 3, 27 -- From: "G. Esteban Fernandez" {g.esteban.fernandez-at-gmail.com} 3, 27 -- To: Confocal Microscopy List {CONFOCALMICROSCOPY-at-lists.umn.edu} , 3, 27 -- microscopy-at-microscopy.com 3, 27 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
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Email: rowland-at-matsys.com Name: Rod Rowland
Organization: MATSYS, Inc.
Title-Subject: [Filtered] JEOL 6100
Message: We have a JEOL 6100 which needs the magnification power supply board replaced. Our chiller stopped working the other day for about an hour and the mag. power supply got so hot half of the wires melted. We are trying to repair it in house but it's not looking good. If anyone can suggest a source for either repair it would be greatly appreciated. The JEOL 6100 model is SM111040-154. The power supply model is SM111040-7A.
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My name is Evan Francis and I'm a recruiter with Schafer Corporation. We're looking to hire a qualified Electron Microscopy Senior Scientist at our lab out in the Sunol, CA area. When you get this, if you're interested, please give me a call at 703-516-6051 or shoot me an email at evan.francis-at-schafercorp.com. Here is the position description on our company website:
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==============================Original Headers============================== 13, 17 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 16 19:24:03 2015 13, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0H1O2Z1025118 13, 17 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2015 19:24:03 -0600 13, 17 -- Message-ID: {54B9B9B3.8080702-at-microscopy.com} 13, 17 -- Date: Fri, 16 Jan 2015 19:24:03 -0600 13, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 13, 17 -- MIME-Version: 1.0 13, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 17 -- Subject: viaWWW:Senior Electron Microscopy Scientist Needed! 13, 17 -- References: {201501161648.t0GGmMCA007483-at-ns.microscopy.com} 13, 17 -- In-Reply-To: {201501161648.t0GGmMCA007483-at-ns.microscopy.com} 13, 17 -- X-Forwarded-Message-Id: {201501161648.t0GGmMCA007483-at-ns.microscopy.com} 13, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
According with my expérience you'll have to change this power supply because it's hard to repair when such problem occurs. This part contains many power transistors and, for this reason, it's water cooled. The most common accident occurs when water flow inside hose and power is switch off. The gradiant between room temperature and cooling water temperature may causes condensation of water (from air humidity) on the surface of power supply and finally destructive short-circuits when power supply is switch on. Week-end and hollydays are dangerous periods where the SEM can be shut down but not water cooling supply. Normally there is a small detector that shut off the board when temperature exceed 70°; it seems this security has not worked for your SEM or this was shorted on the past of your SEM (it's very easy to do from outside of the board and very easy to forget later). Probably JEOL has not such parts in spare because the JSM 6100 is rather old. Another solution is to find this part from a scrapped 6100 (there is today one 6100 on EBay for 12000 dollars). Last solution is to change all the broken parts of the board (there is probably many). This is possible for a good electronics engineer if you can supply him the diagramms. Hope this is help.
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
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==============================Original Headers============================== 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Tue Jan 20 04:12:09 2015 8, 25 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0KAC8Mf031824 8, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2015 04:12:08 -0600 8, 25 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 8, 25 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id t0KAC6b2024513 8, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2015 11:12:06 +0100 8, 25 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 8, 25 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0KAC5OV018825 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT) 8, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2015 11:12:06 +0100 8, 25 -- Message-ID: {54BE2A2B.208-at-univ-nantes.fr} 8, 25 -- Date: Tue, 20 Jan 2015 11:12:59 +0100 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 8, 25 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 8, 25 -- MIME-Version: 1.0 8, 25 -- To: microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] viaWWW:JEOL 6100 repair 8, 25 -- References: {201501170141.t0H1frSX017421-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201501170141.t0H1frSX017421-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-Miltered: at b-mail2 with ID 54BE29F6.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 8, 25 -- X-j-chkmail-Enveloppe: 54BE29F6.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Dear all, anybody out there who has a Usermanual for the Zeiss DSM 960 SEM available in PDF?
Best wishes, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: jlribas-at-us.es Name: Juan Luis Ribas
Organization: Microscopy Facility. Univ of Seville. Spain
Title-Subject: [Filtered] Libra 120. Problem with screens not moving
Message: Good morning everybody, We are having problems with the moving of the small and large screens in our Libra 120. Sometimes (even 3-4 a day) the small screen stay in up position and the large screen in down position and the system (Wintem or iTEM) hangs. That means that neither through the left touch pannel (Small screen or M8) nor the software Wintem buttom have any action when pressed. Also, there is no way to get them back trough iTEM. The rest of the buttons and actions in Wintem are fully functional. The only way to unblock them is to go to off with Libra and on again. Even going to standby has no action.
Someone with more experience in Libra120 handling has experimented this situation before? Thank you very much in advance. Best regards
Juan Luis Ribas
Microscopy Facility. Univ of Seville. Spain
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] ESEM
Message: Hi!
Does any one is having service manual of 'FEI' ESEM Quanta 3D
Rashmi
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Email: SLC6-at-Lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh Microscopy School 2015
Message: Now accepting registrations for the 45th annual Lehigh Microscopy School which will be held June 7-12, 2015. All courses, lecturers, and instrument suppliers will be together for what promises to be a phenomenal week. Course offerings include: Scanning Electron Microscopy and X-ray Microanalysis • Introduction to SEM and EDS for the New Operator • Focused Ion Beam Instrumentation and Applications • Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD Data • Quantitative X-ray Microanalysis: Problem Solving using EDS and WDS Techniques • Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register and pay in full by April 14 for an Early Bird Discount! Contact: Sharon Coe (sharon.coe-at-Lehigh.edu or 610-758-5133). See www.lehigh.edu/microscopy for prices.
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Email: 13qw9-at-queensu.ca Name: Jason Wang
Organization: Queen's University
Title-Subject: [Filtered] how to stream the in-situ TEM video to another computer?
Message: Hello all, what are the normal ways you use for streaming or capturing the in-situ TEM videos by another computer? Since the computer directly connected to TEM is not allowed to install any other software on it, we are not able to take the in-situ video easily. So does connecting to another computer having a Video Capture Card will solve this problem? Thanks for you time!
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Email: gciambrone-at-comcast.net Name: Gary Ciambrone
Organization: Foothill College
Title-Subject: [Filtered] Stage position upon microscope moving/storage
Message: I'm in the process of beginning to teach some community college students the proper way to use a microscope. I have used microscopes in the past and have always moved the stage down away from the objectives (after swinging the low power objective into place) for moving or storing the scope. But then I have run across some information online that implies that this is incorrect. My question therefore is: what is the recommended position for the stage when moving or storing a microscope? Should the stage be moved as far from the objectives as possible, or should the stage be moved all the way to the top position after the low power objective is swung into place? Thank you very much.
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*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
} realname - hatem alharbi } Email - hatem.alharbi-at-my.mcphs.edu } ORGANIZATION - mcphs } EDUCATION - Graduate College } LOCATION - boston,MA,USA } SUBJECT_OF_QUESTION - flow cytometry + microscopy } QUESTION - Do you have comparison between flow cytomety devices please } how many flow cytometry + microscopy devices there and what is the best one of them }
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Wed Jan 21 07:07:39 2015 5, 32 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0LD7do4005074 5, 32 -- for {microscopy-at-microscopy.com} ; Wed, 21 Jan 2015 07:07:39 -0600 5, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 5, 32 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0LD7bdL003839 5, 32 -- for {microscopy-at-microscopy.com} ; Wed, 21 Jan 2015 08:07:37 -0500 5, 32 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 5, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.195.1; Wed, 21 Jan 2015 08:07:38 -0500 5, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 32 -- cas2.central.cmich.local (141.209.15.141) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.195.1; Wed, 21 Jan 2015 08:07:38 -0500 5, 32 -- Message-ID: {54BFA491.3090202-at-cmich.edu} 5, 32 -- Date: Wed, 21 Jan 2015 08:07:29 -0500 5, 32 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: micro {microscopy-at-microscopy.com} 5, 32 -- Subject: Re: Ask-A-Microscopist: any flow cytometers with microscopes? 5, 32 -- References: {831116267.1397.1421803102220.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 32 -- In-Reply-To: {831116267.1397.1421803102220.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 32 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit 5, 32 -- X-Originating-IP: [141.209.2.100] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 05NGB7B3x - cc2422988384 - 20150121 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Hooke College of Applied Sciences, located in Westmont, IL, is offering a Scanning Electron Microscopy short course March 23-27, 2015. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.
For further SEM training details and registration information, please follow the link below:
Chris Gorman | Admissions Specialist Hooke College of Applied Sciences, LLC . 850 Pasquinelli Drive . Westmont, IL 60559 P. 630.887.7100 | F. 630.887.7412 | www.hookecollege.com
==============================Original Headers============================== 8, 41 -- From CGorman-at-hookecollege.com Thu Jan 22 10:44:46 2015 8, 41 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 8, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0MGikow001847 8, 41 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 10:44:46 -0600 8, 41 -- X-ASG-Debug-ID: 1421945083-07bbd410cb57fb0001-4CH8be 8, 41 -- Received: from TMGEX2.tmg.mccrone.com ([192.168.101.54]) by spam.mccrone.com with ESMTP id TJB1hhP0WeDoLO5x for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 10:44:43 -0600 (CST) 8, 41 -- X-Barracuda-Envelope-From: CGorman-at-hookecollege.com 8, 41 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by 8, 41 -- TMGEX2.tmg.mccrone.com (192.168.101.74) with Microsoft SMTP Server (TLS) id 8, 41 -- 15.0.913.22; Thu, 22 Jan 2015 10:44:42 -0600 8, 41 -- Received: from TMGEX1.tmg.mccrone.com ([::1]) by TMGEX1.tmg.mccrone.com 8, 41 -- ([fe80::5c:a323:e462:160%14]) with mapi id 15.00.0913.011; Thu, 22 Jan 2015 8, 41 -- 10:44:42 -0600 8, 41 -- From: Christine Gorman {CGorman-at-hookecollege.com} 8, 41 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 8, 41 -- Subject: Scanning Electron Microscopy short course March 23-27, 2015 8, 41 -- Thread-Topic: Scanning Electron Microscopy short course March 23-27, 2015 8, 41 -- X-ASG-Orig-Subj: Scanning Electron Microscopy short course March 23-27, 2015 8, 41 -- Thread-Index: AdA2Yp7amkh3/6q5SL2qvUjXtDzI0g== 8, 41 -- Date: Thu, 22 Jan 2015 16:44:41 +0000 8, 41 -- Message-ID: {764a26b9fc6146c39685f7b7eeada80d-at-TMGEX1.tmg.mccrone.com} 8, 41 -- Accept-Language: en-US 8, 41 -- Content-Language: en-US 8, 41 -- X-MS-Has-Attach: 8, 41 -- X-MS-TNEF-Correlator: 8, 41 -- x-originating-ip: [192.168.101.154] 8, 41 -- Content-Type: text/plain; charset="iso-8859-1" 8, 41 -- MIME-Version: 1.0 8, 41 -- X-Barracuda-Connect: UNKNOWN[192.168.101.54] 8, 41 -- X-Barracuda-Start-Time: 1421945083 8, 41 -- X-Barracuda-URL: http://spam.mccrone.com:80/cgi-mod/mark.cgi 8, 41 -- X-Virus-Scanned: by bsmtpd at mccrone.com 8, 41 -- X-Barracuda-BRTS-Status: 1 8, 41 -- X-Barracuda-Spam-Score: 0.00 8, 41 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 8, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.14531 8, 41 -- Rule breakdown below 8, 41 -- pts rule name description 8, 41 -- ---- ---------------------- -------------------------------------------------- 8, 41 -- Content-Transfer-Encoding: 8bit 8, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0MGikow001847 ==============================End of - Headers==============================
Hello We cannot align the aperture in the JSM5600LV - SEM . Using the wobbler tool we cannot correct horizontal movement, in all 3 aperture positions.. Looks like the alignment point lies outside the aperture openings. The problem worsens with increasing tilt angle and affects the resolution (5K looks like 15K). I cleaned the upper column up to the aperture level and the aperture, but didn't help (it was not dirty anyway). However, last time I changed filament I noticed some little white crisps around the Wehnelt.orifice. Is it likely to have contamination of the lower parts of the column or somethig else? Any advise will be much appreciated Thanks yorgos
I don`t expect the dirt on the wehnelt aperture to be much of a problem, but better clean it away. Are you sure your final aperture strip (or do you have single apertures?) are not moving in the aperturestrip-holder? Did you check that the holder is perfectly clean? Since you say you have the same problem with all three aperture openings it might also be a problem with the astigmatism correction voltage going to the coils. If you have the schematics, you can measure this at the plug.
All the best, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 22.01.15 um 17:54 schrieb eikonika-at-otenet.gr: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello } We cannot align the aperture in the JSM5600LV - SEM . Using the wobbler tool } we cannot correct horizontal movement, in all 3 aperture positions.. Looks } like the alignment point lies outside the aperture openings. The problem } worsens with increasing tilt angle and affects the resolution (5K looks like } 15K). I cleaned the upper column up to the aperture level and the aperture, } but didn't help (it was not dirty anyway). However, last time I changed } filament I noticed some little white crisps around the Wehnelt.orifice. } Is it likely to have contamination of the lower parts of the column or } somethig else? Any advise will be much appreciated } Thanks } yorgos } } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.net www.aim.cat } ************************************ } } } ==============================Original Headers============================== } 4, 22 -- From eikonika-at-otenet.gr Thu Jan 22 10:50:05 2015 } 4, 22 -- Received: from echidna.otenet.gr (smtp-out33.otenet.gr [83.235.69.33]) } 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0MGo5JF005234 } 4, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 10:50:05 -0600 } 4, 22 -- Received: from pegasus (ppp-2-84-173-165.home.otenet.gr [2.84.173.165]) } 4, 22 -- by echidna.otenet.gr (ESMTP) with SMTP } 4, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 18:50:01 +0200 (EET) } 4, 22 -- Message-ID: {8879A4BBAFB44336B6364198CE575588-at-pegasus} } 4, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr} } 4, 22 -- To: {microscopy-at-microscopy.com} } 4, 22 -- Subject: JSM5600LV beam alignment problem } 4, 22 -- Date: Thu, 22 Jan 2015 18:49:58 +0200 } 4, 22 -- MIME-Version: 1.0 } 4, 22 -- Content-Type: text/plain; } 4, 22 -- format=flowed; } 4, 22 -- charset="iso-8859-1"; } 4, 22 -- reply-type=original } 4, 22 -- Content-Transfer-Encoding: 7bit } 4, 22 -- X-Priority: 3 } 4, 22 -- X-MSMail-Priority: Normal } 4, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 } 4, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 23 -- From stefan.diller-at-t-online.de Thu Jan 22 11:49:56 2015 9, 23 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0MHntHe009757 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 11:49:55 -0600 9, 23 -- Received: from fwd24.aul.t-online.de (fwd24.aul.t-online.de [172.20.26.129]) 9, 23 -- by mailout04.t-online.de (Postfix) with SMTP id 8F26A4822A5 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 18:49:54 +0100 (CET) 9, 23 -- Received: from mac-pro.local (GibinyZD8hrn4wQ7a8Fv1AMjC0QtMtGrE4MWt7NFC9WFer452eNbWxXhLjwIjxowEA-at-[91.58.164.206]) by fwd24.t-online.de 9, 23 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) 9, 23 -- esmtp id 1YELt6-2xMJvc0; Thu, 22 Jan 2015 18:49:48 +0100 9, 23 -- Message-ID: {54C1383B.7040405-at-t-online.de} 9, 23 -- Date: Thu, 22 Jan 2015 18:49:47 +0100 9, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 9, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 9, 23 -- MIME-Version: 1.0 9, 23 -- To: microscopy-at-microscopy.com 9, 23 -- Subject: Re: [Microscopy] JSM5600LV beam alignment problem 9, 23 -- References: {201501221654.t0MGs6df013480-at-ns.microscopy.com} 9, 23 -- In-Reply-To: {201501221654.t0MGs6df013480-at-ns.microscopy.com} 9, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- X-ID: GibinyZD8hrn4wQ7a8Fv1AMjC0QtMtGrE4MWt7NFC9WFer452eNbWxXhLjwIjxowEA 9, 23 -- X-TOI-MSGID: 4a043bc0-c1a2-444b-8ce9-49d94abc2aa4 ==============================End of - Headers==============================
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} Below is the result of your form, submitted on Thursday, January 22, 2015 at 03:33:32 PM. } } realname - Giovanni De Caro, MD } Email - neurostar-at-outlook.it } ORGANIZATION - Museo Scienze Naturali Montalbo´ } EDUCATION - Graduate College } LOCATION - Campobasso, Italy } SUBJECT_OF_QUESTION - Spot Insight QE digital camera } QUESTION - Hi all, } } I have acquired secondhand a SPOT Insight QE digital camera , model 4.2 , S/N 221276. The camera appears to be in good overall shape, it powers up and the fan goes; unfortunately I do not have the manual, the software and the data cable. Hs someone of you experience in operating this camera and can give me some hints about how to find the lacking components? The manual is available online on the Spot website, but I do not know if this is the monochrome or the color camera model; about the data cable, maybe it can be found on the web and , to end, maybe there is a shareware software that can be used to get pictures from this camera on my PC. } I look forward receiving answers form you and remain.
==============================Original Headers============================== 5, 33 -- From oshel1pe-at-cmich.edu Thu Jan 22 15:46:34 2015 5, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0MLkXRQ006442 5, 33 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 15:46:34 -0600 5, 33 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0MLkVF8001988 5, 33 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2015 16:46:31 -0500 5, 33 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 5, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 33 -- (TLS) id 14.3.195.1; Thu, 22 Jan 2015 16:46:30 -0500 5, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 33 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 5, 33 -- 14.3.195.1; Thu, 22 Jan 2015 16:46:30 -0500 5, 33 -- Message-ID: {54C16FB6.3080200-at-cmich.edu} 5, 33 -- Date: Thu, 22 Jan 2015 16:46:30 -0500 5, 33 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 33 -- MIME-Version: 1.0 5, 33 -- To: micro {microscopy-at-microscopy.com} 5, 33 -- Subject: Re: Ask-A-Microscopist: manual, software, and cables for Spot Insight 5, 33 -- QE camera 5, 33 -- References: {1784974025.5285.1421958813550.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 33 -- In-Reply-To: {1784974025.5285.1421958813550.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 33 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 5, 33 -- Content-Transfer-Encoding: 8bit 5, 33 -- X-Originating-IP: [141.209.2.100] 5, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 33 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 33 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 33 -- X-CanItPRO-Stream: default 5, 33 -- X-Canit-Stats-ID: 02NH9Kvts - faea7a0ad411 - 20150122 5, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
May I suggest you take out the aperture strip and see if you can align the instrument to your satisfaction. The problem may be in another part of the column resulting in an apparent aperture problem. I too do not expect the dirt on the wehnelt to be a real problem.
Try this and let us know what happens. You will need to use small spot sizes to obtain a reasonable image quality in the test.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: 22 January 2015 17:51 To: protrain-at-emcourses.com
X-from: huixin.xiu-at-googlemail.com
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Email: huixin.xiu-at-googlemail.com Name: Helen Xiu
Organization: FEI
Title-Subject: [Filtered] chirality identification using TEM tomography
Message: Dear all,
We would like to identify the chirality of their spiral material using TEM tomography in a FEI Tecnai F20 with Explore 3D software. After TEM tomography acquisition, we used Inspect3D 3.1 to reconstruct the data, however, we found using different direction (Y direction or Z direction) to reconstruct volume, we got opposite chirality of their spiral. We compared with the chirality got from SEM image, finding that reconstructing along Y direction gives the right chirality, but as far as I know, Inspect3D4.0 will reconstruct the volume along z direction as a default. Can anybody share your expertise with tomography reconstruction or any related experience? Many thanks!
Best regards, Helen
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==============================Original Headers============================== 19, 17 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 23 07:24:27 2015 19, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 19, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0NDOR0j026794 19, 17 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2015 07:24:27 -0600 19, 17 -- Message-ID: {54C24B8B.4040608-at-microscopy.com} 19, 17 -- Date: Fri, 23 Jan 2015 07:24:27 -0600 19, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 19, 17 -- MIME-Version: 1.0 19, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 17 -- Subject: viaWWW:chirality identification using TEM tomography 19, 17 -- References: {201501230637.t0N6bd0b020124-at-ns.microscopy.com} 19, 17 -- In-Reply-To: {201501230637.t0N6bd0b020124-at-ns.microscopy.com} 19, 17 -- X-Forwarded-Message-Id: {201501230637.t0N6bd0b020124-at-ns.microscopy.com} 19, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 19, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I'm trying to analyze some STEM-EDS data acquired using a JEOL ARM-200CF. The data includes maps of several edges and is stored in ".img" and ".map" formats. I would like to import this data into the Cornell Spectrum Imager (CSI) program for analysis using PCA and other routines, but there doesn't seem to be a way to do so.
Can anyone offer advice on dealing with JEOL EDS data? Are there any viewers or ImageJ plugins that I could use to read/convert this data into the proper format?
Thanks for your help!
__________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: Monday, January 26, 2015 10:07 AM To: John Mardinly
Hello everyone,
I'm trying to analyze some STEM-EDS data acquired using a JEOL ARM-200CF. The data includes maps of several edges and is stored in ".img" and ".map" formats. I would like to import this data into the Cornell Spectrum Imager (CSI) program for analysis using PCA and other routines, but there doesn't seem to be a way to do so.
Can anyone offer advice on dealing with JEOL EDS data? Are there any viewers or ImageJ plugins that I could use to read/convert this data into the proper format?
Thanks for your help!
__________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
The University of Missouri - Columbia is looking for a new staff member for our Molecular Cytology Core (MCC) facility. The MCC is an instrumentation resource and service facility for all type of light microscopic imaging. Last year the MCC served approximately 200 clients from over 80 different labs located in 30 departments on our campus. Information on our facility can be found at http://biotech.rnet.missouri.edu/mcc/
The ideal candidate will have extensive experience in confocal microscopy. We are particularly interested in individuals with experience making use of FLIM or FLIM/FRET technology.
Job responsibilities include:
* Supervise and train users (faculty, postdoctoral fellows, and graduate students) in the use of sophisticated microscopy instruments and software. * Develop new protocols involving single photon and multi-photon confocal microscopy and FLIM/FRET applications. * Maintain instruments and a clean, safe and orderly work environment. * Assist in some service work for clients. * Prepare monthly billing statement; maintain inventory and order supplies, dispense materials to clients.
Although some applicants to this position may hold a PhD degree, it is not a requirement for those with significant experience in a core or imaging lab. To apply for the job, visit http://hrs.missouri.edu/find-a-job/staff/index.php and search for "Research Specialist II" - the job ID number is 15116. If you have questions, you can contact either the MCC's Director, Dr. Tom Phillips (phillipst-at-missouri.edu) or the core's Associate Director, Dr. Alexander Jurkevic (jurkevica-at-missouri.edu). The job will remain open until a suitable candidate is found.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax)
==============================Original Headers============================== 7, 30 -- From PhillipsT-at-missouri.edu Mon Jan 26 17:36:22 2015 7, 30 -- Received: from um-nip4-missouri-out.um.umsystem.edu (um-nip4-missouri-out.um.umsystem.edu [198.209.49.177]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0QNaMiu021944 7, 30 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2015 17:36:22 -0600 7, 30 -- X-IronPort-Anti-Spam-Filtered: true 7, 30 -- X-IronPort-Anti-Spam-Result: A2HJBQDizsZU/9SeoM9AFwODBlJZBMZBhXECgRpDAQEBAQEDeoI+OAgEHQITWQEBBDpNAgIBKhQQFhwlAQEEEwiIJA03zxYBhFIBCgEBAQEBARsEjWeBXDgRgwWBEwWOboNLgg2FFIpzhX4iggCBbm8BgUN+AQEB 7, 30 -- X-IPAS-Result: A2HJBQDizsZU/9SeoM9AFwODBlJZBMZBhXECgRpDAQEBAQEDeoI+OAgEHQITWQEBBDpNAgIBKhQQFhwlAQEEEwiIJA03zxYBhFIBCgEBAQEBARsEjWeBXDgRgwWBEwWOboNLgg2FFIpzhX4iggCBbm8BgUN+AQEB 7, 30 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 7, 30 -- by um-nip4-exch-relay.um.umsystem.edu with ESMTP; 26 Jan 2015 17:36:21 -0600 7, 30 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.75]) by 7, 30 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0210.002; 7, 30 -- Mon, 26 Jan 2015 17:36:21 -0600 7, 30 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 7, 30 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 7, 30 -- Subject: Staff position in LM core 7, 30 -- Thread-Topic: Staff position in LM core 7, 30 -- Thread-Index: AdA5v5Zrxzj78aj5TSClRg6cPC++CQAARUIw 7, 30 -- Date: Mon, 26 Jan 2015 23:36:20 +0000 7, 30 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DE7023734-at-UM-MBX-T02.um.umsystem.edu} 7, 30 -- References: {CB463A8DE3499A4CA204087E4EEB363DE70235FE-at-UM-MBX-T02.um.umsystem.edu} 7, 30 -- In-Reply-To: {CB463A8DE3499A4CA204087E4EEB363DE70235FE-at-UM-MBX-T02.um.umsystem.edu} 7, 30 -- Accept-Language: en-US 7, 30 -- Content-Language: en-US 7, 30 -- X-MS-Has-Attach: 7, 30 -- X-MS-TNEF-Correlator: 7, 30 -- x-originating-ip: [128.206.81.115] 7, 30 -- Content-Type: text/plain; charset="us-ascii" 7, 30 -- MIME-Version: 1.0 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0QNaMiu021944 ==============================End of - Headers==============================
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Email: sameesh-at-uga.edu Name: Sam Gonzalez
Organization: University of Georgia
Title-Subject: [Filtered] Fixation of mitochondria
Message: Okay I am very confused about how to go about fixing a sample of isolated mitochondria for TEM.
All the procedures seem to be very vague about "washing the pellet". To me, this implies resuspending the pellet in buffer and then spinning it back down. But the procedures I have looked at never mention the g force for this step, but does specify the time So I would assume just use the g force of the last centrifuge step performed, but this step involves addition of agarose solution to enrobe the pellet, so it seems like this would be a distinct and unrelated step.
Also, the addition of agarose is never mentioned again, so would I add it to the buffer for the washing steps, assuming washing involves more centrifugation?
Alternatively, does washing the pellet just mean inundating the pellet with buffer and then pouring it off? If that is the case, how do i do this for 20 minutes? Just let the buffer sit on the pellet for 20 minutes then pour it off?
Thanks, Sam Very confused masters student
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 26 18:57:44 2015 15, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0R0vhbh011871 15, 17 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2015 18:57:44 -0600 15, 17 -- Message-ID: {54C6E288.9040005-at-microscopy.com} 15, 17 -- Date: Mon, 26 Jan 2015 18:57:44 -0600 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:Fixation of mitochondria 15, 17 -- References: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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We have a custom holder for the JEOL 2100 but have been having problems with insertion and extraction. The holder does not get "sucked in" by the vacuum after the first and second rotations. It actually requires a little pushing, and sometimes extraction is very difficult, requiring more force than standard holder. The puzzling thing is that the holder works perfectly in a JEOL 2010F.
The holder manufacturer has rechecked all physical dimensions of the holder and the o-rings, finding them within tolerance. JEOL also has taken back the goniometer on repeated occasions but has not found a solution. Oddly, a loaner goniometer JEOL installs when they take ours away, happens to work without problems.
Last incident we had, the vacuum was breached upon holder extraction. While pulling the holder, the outer gasket on the goniometer was extracted too. What is the most possible cause for this? We see wear marks on the alignment pin of the holder.
Thanks!
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==============================Original Headers============================== 16, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 26 18:58:43 2015 16, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0R0whxX012825 16, 17 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2015 18:58:43 -0600 16, 17 -- Message-ID: {54C6E2C3.9010209-at-microscopy.com} 16, 17 -- Date: Mon, 26 Jan 2015 18:58:43 -0600 16, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 16, 17 -- MIME-Version: 1.0 16, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 17 -- Subject: viaWWW:Holder Insertion/Extraction problems JEOL 2100 16, 17 -- References: {201501261431.t0QEVwfb004278-at-ns.microscopy.com} 16, 17 -- In-Reply-To: {201501261431.t0QEVwfb004278-at-ns.microscopy.com} 16, 17 -- X-Forwarded-Message-Id: {201501261431.t0QEVwfb004278-at-ns.microscopy.com} 16, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
It sounds like a goniometer problem. Wear marks on the alignment pin is not good because the inner tube of the goniometer can be scratched. This tube is a rotating part that moves with the holder and a scuffing is possible between this tube and the internal wall of the goniometer. Scratchs on the inner tube can weak the vacuum sealing during the movement of the holder. If this hypothesis is the good one it's necessary to remove this tube (It's a job for JEOL engineer) to get it into shape of the goniometer wall (by soft abrasion with Pikal for example). If there is scratchs this inner tube must be replaced. This is a tricky job and expensive part. Each vacuum leak during holder push/pull movement is bad experience for the ion pump. Hope this is help.
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
Le 27/01/2015 02:13, microscopylistserver-noreply-at-microscopy.com a écrit : } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } X-from: robernal-at-u.northwestern.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } please copy both robernal-at-u.northwestern.edu as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } } Email: robernal-at-u.northwestern.edu } Name: Rodrigo Bernal } } Organization: Northwestern University } } Title-Subject: [Filtered] Holder Insertion/Extraction problems JEOL } 2100 } } Message: Hello All, } } We have a custom holder for the JEOL 2100 but have been having } problems with insertion and extraction. The holder does not get } "sucked in" by the vacuum after the first and second rotations. It } actually requires a little pushing, and sometimes extraction is very } difficult, requiring more force than standard holder. The puzzling } thing is that the holder works perfectly in a JEOL 2010F. } } The holder manufacturer has rechecked all physical dimensions of the } holder and the o-rings, finding them within tolerance. JEOL also has } taken back the goniometer on repeated occasions but has not found a } solution. Oddly, a loaner goniometer JEOL installs when they take } ours away, happens to work without problems. } } Last incident we had, the vacuum was breached upon holder extraction. } While pulling the holder, the outer gasket on the goniometer was } extracted too. What is the most possible cause for this? We see wear } marks on the alignment pin of the holder. } } Thanks! } } Login Host: 158.130.72.175 Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Tue Jan 27 02:30:50 2015 7, 26 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0R8Un4L031783 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 02:30:49 -0600 7, 26 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 7, 26 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id t0R8UlIj013046; 7, 26 -- Tue, 27 Jan 2015 09:30:47 +0100 7, 26 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 7, 26 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0R8UkfC018245 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT); 7, 26 -- Tue, 27 Jan 2015 09:30:46 +0100 7, 26 -- Message-ID: {54C74CB6.3070307-at-univ-nantes.fr} 7, 26 -- Date: Tue, 27 Jan 2015 09:30:46 +0100 7, 26 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 7, 26 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 7, 26 -- MIME-Version: 1.0 7, 26 -- To: microscopy-at-microscopy.com, robernal-at-u.northwestern.edu 7, 26 -- Subject: Re: [Microscopy] viaWWW:Holder Insertion/Extraction problems JEOL 7, 26 -- 2100 7, 26 -- References: {201501270113.t0R1DlfP005080-at-ns.microscopy.com} 7, 26 -- In-Reply-To: {201501270113.t0R1DlfP005080-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-Miltered: at b-mail2 with ID 54C74CB7.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 7, 26 -- X-j-chkmail-Enveloppe: 54C74CB7.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Liquid agarose is added to make processing easier. Processing some samples such as cell suspensions and organelles can be tricky, especially when it a very small quantity, as the majority of the sample gets lost during all the washing steps - plus it can get very hard to pellet them in the thick embedding resins. Adding liquid agarose in the earlier stages - then leaving it to cool so that it sets hard - embeds the samples in a larger, gel like pellet that can then be processed through as if it were a larger sample - without the need for centrifugation at each step.
When I process organelles, I usually fix in primary fixative, wash in buffer, centrifuge it down into a good pellet, remove as much supernatant as possible, then add a small drop of warm (not too hot) agarose (3%), making sure there is no air bubbles between it and the pellet, leave in a warm waterbath for 10 minutes to allow the agarose to infuse with the pellet, then move to the fridge until the agarose is completely set. I then carefully remove the agarose/sample from the tube, and if necessary, use a razor blade to cut it into 1mm2 pieces for further processing. You should then have some nice cubes of agarose/sample (or maybe just a small smear of sample in the tip if you're processing organelles or a difficult sample) that you can process through without the need for further spinning.
Sometimes, despite all my best efforts, the sample pellet gets left behind in the Eppendorf when I remove the set agarose gel. In this instance, I carefully slip the pellet onto a glass slide, and sandwich it between the agarose that way. If anyone has any helpful hints to combat this, it would be much appreciated!
Hope that makes more sense Sam?
Nat
Miss Natalie Allcock CBS Electron Microscopy Facility College of Medicine, Biological Sciences and Psychology Adrian Building University of Leicester LE1 7RH
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: 27 January 2015 01:21 To: Allcock, Natalie S.
X-from: sameesh-at-uga.edu
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Email: sameesh-at-uga.edu Name: Sam Gonzalez
Organization: University of Georgia
Title-Subject: [Filtered] Fixation of mitochondria
Message: Okay I am very confused about how to go about fixing a sample of isolated mitochondria for TEM.
All the procedures seem to be very vague about "washing the pellet". To me, this implies resuspending the pellet in buffer and then spinning it back down. But the procedures I have looked at never mention the g force for this step, but does specify the time So I would assume just use the g force of the last centrifuge step performed, but this step involves addition of agarose solution to enrobe the pellet, so it seems like this would be a distinct and unrelated step.
Also, the addition of agarose is never mentioned again, so would I add it to the buffer for the washing steps, assuming washing involves more centrifugation?
Alternatively, does washing the pellet just mean inundating the pellet with buffer and then pouring it off? If that is the case, how do i do this for 20 minutes? Just let the buffer sit on the pellet for 20 minutes then pour it off?
Thanks, Sam Very confused masters student
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 26 18:57:44 2015 15, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0R0vhbh011871 15, 17 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2015 18:57:44 -0600 15, 17 -- Message-ID: {54C6E288.9040005-at-microscopy.com} 15, 17 -- Date: Mon, 26 Jan 2015 18:57:44 -0600 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:Fixation of mitochondria 15, 17 -- References: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201501262058.t0QKwm0j019204-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 33, 32 -- From nsa2-at-leicester.ac.uk Tue Jan 27 04:58:07 2015 33, 32 -- Received: from bontebok.le.ac.uk (bontebok.le.ac.uk [143.210.16.36]) 33, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0RAw77T020747 33, 32 -- for {Microscopy-at-Microscopy.com} ; Tue, 27 Jan 2015 04:58:07 -0600 33, 32 -- Received: from host-133-195.it.le.ac.uk ([143.210.133.195]:64339 helo=exp-casht-i3.uol.le.ac.uk) 33, 32 -- by bontebok.le.ac.uk with esmtps (TLS1.0:RSA_AES_256_CBC_SHA1:256) 33, 32 -- (Exim 4.80) 33, 32 -- (envelope-from {nsa2-at-leicester.ac.uk} ) 33, 32 -- id 1YG3qO-0006zJ-2v 33, 32 -- for Microscopy-at-Microscopy.com; Tue, 27 Jan 2015 10:58:04 +0000 33, 32 -- Received: from EXP-DAG1-N2.uol.le.ac.uk ([::1]) by exp-casht-i3.uol.le.ac.uk 33, 32 -- ([143.210.133.195]) with mapi id 14.03.0195.001; Tue, 27 Jan 2015 10:58:05 33, 32 -- +0000 33, 32 -- From: "Allcock, Natalie S." {nsa2-at-leicester.ac.uk} 33, 32 -- To: "'Microscopy-at-Microscopy.com'" {Microscopy-at-Microscopy.com} 33, 32 -- Subject: RE: [Microscopy] viaWWW:Fixation of mitochondria 33, 32 -- Thread-Topic: [Microscopy] viaWWW:Fixation of mitochondria 33, 32 -- Thread-Index: AQHQOc+MQrqNW+MkVki+cYQMgojOwpzTwUFA 33, 32 -- Date: Tue, 27 Jan 2015 10:58:04 +0000 33, 32 -- Message-ID: {C31045C47F49444BA76584B3DC6020CE16C1BFC7-at-exp-dag1-n2.uol.le.ac.uk} 33, 32 -- References: {201501270121.t0R1L5ql019062-at-ns.microscopy.com} 33, 32 -- In-Reply-To: {201501270121.t0R1L5ql019062-at-ns.microscopy.com} 33, 32 -- Accept-Language: en-GB, en-US 33, 32 -- Content-Language: en-US 33, 32 -- X-MS-Has-Attach: 33, 32 -- X-MS-TNEF-Correlator: 33, 32 -- x-originating-ip: [143.210.155.106] 33, 32 -- Content-Type: text/plain; charset="us-ascii" 33, 32 -- MIME-Version: 1.0 33, 32 -- X-UoL-Id: 6cee7a9a2660ae1862e174211234a0d7-at-1YG3qO-0006zJ-2v-at-bontebok.le.ac.uk 33, 32 -- Content-Transfer-Encoding: 8bit 33, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0RAw77T020747 ==============================End of - Headers==============================
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Email: alice-at-rms.org.uk Name: Alice Pyper
Organization: Royal Microscopical Society
Title-Subject: [Filtered] RMS Electron Microscopy Spring School
Message: The Royal Microscopical Society Electron Microscopy Spring School is talking place at Leeds University, England, from 23rd to 27th March 2015.
The School offers education on the theory and practice of scanning and transmission electron microscopy. The course covers, imaging, diffraction, and chemical microanalysis, as well as the important area of specimen preparation. It consists of lectures, demonstrations and hands on practical periods, as well as offering plenty of opportunity to ask questions, and to arrange time for specific demonstrations. There are two basic streams, Physical and Engineering Sciences, and Life Sciences, with an additional stream for those wishing to concentrate on TEM.
The course is designed to suit both beginners and experienced microscopists from all areas of academia and industry.
We are pleased to have Steve Chapman give us an insight into a life in electron microscopy, by presenting the annual lecture which he has entitled "50 years and a Million Miles"
The contact for more information is alice-at-rms.org.uk
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 27 07:10:33 2015 15, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0RDAX27009286 15, 17 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 07:10:33 -0600 15, 17 -- Message-ID: {54C78E49.50806-at-microscopy.com} 15, 17 -- Date: Tue, 27 Jan 2015 07:10:33 -0600 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: microscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:RMS Electron Microscopy Spring School 15, 17 -- References: {201501271005.t0RA5prE020071-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201501271005.t0RA5prE020071-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201501271005.t0RA5prE020071-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Does anyone have a PDF of the Leica RM2065 operator's manual? We need to set up and use our old unit but the manual is missing.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 11, 26 -- From lettj-at-ent.wustl.edu Tue Jan 27 10:35:22 2015 11, 26 -- Received: from wusm-pcf.wustl.edu (pcf-ironmail02.wusm-pcf.wustl.edu [128.252.17.82]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0RGZLsY031915 11, 26 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 10:35:21 -0600 11, 26 -- Received: from ([10.39.163.68]) 11, 26 -- by pcf-ironmail02.wusm-pcf.wustl.edu with ESMTP with TLS id 5THYGJ1.29579809; 11, 26 -- Tue, 27 Jan 2015 10:34:06 -0600 11, 26 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 11, 26 -- PCFHUB01.wusm-pcf.wustl.edu ([10.39.163.64]) with mapi id 14.03.0181.006; 11, 26 -- Tue, 27 Jan 2015 10:35:18 -0600 11, 26 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 11, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 26 -- Subject: LM -- Leica RM2065 rotary microtome 11, 26 -- Thread-Topic: LM -- Leica RM2065 rotary microtome 11, 26 -- Thread-Index: AdA6Tt7iq792OVPSQhGCrkbeEtJ8og== 11, 26 -- Date: Tue, 27 Jan 2015 16:35:17 +0000 11, 26 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E1353220129605-at-pcfmbx01.wusm-pcf.wustl.edu} 11, 26 -- Accept-Language: en-US 11, 26 -- Content-Language: en-US 11, 26 -- X-MS-Has-Attach: 11, 26 -- X-MS-TNEF-Correlator: 11, 26 -- x-originating-ip: [10.39.135.187] 11, 26 -- Content-Type: text/plain; charset="iso-8859-1" 11, 26 -- MIME-Version: 1.0 11, 26 -- Content-Transfer-Encoding: 8bit 11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0RGZLsY031915 ==============================End of - Headers==============================
My second question of the day: Does anyone have a PDF of the Reichert Knifemaker II? (I guess the Reichert-Jung 705202 is almost identical.)
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 12, 26 -- From lettj-at-ent.wustl.edu Tue Jan 27 16:51:27 2015 12, 26 -- Received: from wusm-pcf.wustl.edu (pcf-ironmail01.wusm-pcf.wustl.edu [128.252.17.163]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0RMpRAR025736 12, 26 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 16:51:27 -0600 12, 26 -- Received: from ([10.39.163.69]) 12, 26 -- by pcf-ironmail01.wusm-pcf.wustl.edu with ESMTP with TLS id 4THYGJ1.108462541; 12, 26 -- Tue, 27 Jan 2015 16:51:24 -0600 12, 26 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 12, 26 -- DRPCFHUB01.wusm-pcf.wustl.edu ([10.39.163.69]) with mapi id 14.03.0181.006; 12, 26 -- Tue, 27 Jan 2015 16:51:24 -0600 12, 26 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 12, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 26 -- Subject: TEM -- Reichert Knifemaker II 12, 26 -- Thread-Topic: TEM -- Reichert Knifemaker II 12, 26 -- Thread-Index: AdA6g3f4VMmJQJPIT0ufOKfkLLThhA== 12, 26 -- Date: Tue, 27 Jan 2015 22:51:23 +0000 12, 26 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E135322012A76A-at-pcfmbx01.wusm-pcf.wustl.edu} 12, 26 -- Accept-Language: en-US 12, 26 -- Content-Language: en-US 12, 26 -- X-MS-Has-Attach: 12, 26 -- X-MS-TNEF-Correlator: 12, 26 -- x-originating-ip: [10.39.135.187] 12, 26 -- Content-Type: text/plain; charset="iso-8859-1" 12, 26 -- MIME-Version: 1.0 12, 26 -- Content-Transfer-Encoding: 8bit 12, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0RMpRAR025736 ==============================End of - Headers==============================
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Email: neurostar-at-outlook.it Name: Giovanni De Caro
Organization: Museo Scienze Naturali Montalbo´
Title-Subject: [Filtered] Spot microscope camera - help needed
Message: Hi, I am the scientific director of a no profit Science Museum for youngsters based in Southern Italy (http://web.tiscali.it/exploratorium). We have been given an old SPOT Insight QE color digital camera , model 4.2 , S/N 221276 (Diagnostic Instruments inc.) and we would like to use it for our educational programs. The camera came to us with its power supply, it powers up fine and the fan runs, the only problem is that we did not get the data cable and the proprietary PCI card to run it. We did contact Diagnostic Instruments but they asked about 2000 USD for the card and the cable, so we are looking for a different solution. May you help us? Are there universal PCI cards that can be modified to work with this camera? Thank you for your kind help, I look forward receiving your reply and remain Giovanni De Caro, MD Italy
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 27 20:03:08 2015 14, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S2383w015214 14, 17 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 20:03:08 -0600 14, 17 -- Message-ID: {54C84358.1060506-at-microscopy.com} 14, 17 -- Date: Tue, 27 Jan 2015 20:03:04 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:Spot microscope camera - help needed 14, 17 -- References: {201501272104.t0RL4pUM023977-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201501272104.t0RL4pUM023977-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201501272104.t0RL4pUM023977-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I wouldn’t be surprised if it is a pretty generic RS-422 parallel interface like AIA. You might talk to the guys at EDT (Engineering Design Team, Inc), they make interfaces for a lot of cameras.
There is a card on ebay that is probably for your camera, search fir "Diagnostic Instruments Inc P/N 0457†and it should come up. Make them an offer.
-Jerry
} On Jan 27, 2015, at 6:21 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } SPOT Insight QE
==============================Original Headers============================== 7, 36 -- From jerry.biehler-at-gmail.com Tue Jan 27 20:34:31 2015 7, 36 -- Received: from mail-ig0-f180.google.com (mail-ig0-f180.google.com [209.85.213.180]) 7, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S2YV8v002997 7, 36 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 20:34:31 -0600 7, 36 -- Received: by mail-ig0-f180.google.com with SMTP id b16so8562202igk.1 7, 36 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 18:34:30 -0800 (PST) 7, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 36 -- d=gmail.com; s=20120113; 7, 36 -- h=content-type:mime-version:subject:from:in-reply-to:date:cc 7, 36 -- :content-transfer-encoding:message-id:references:to; 7, 36 -- bh=TKgH1EZGDItrHMCw+Dh51WKysz+KSdaoYF9wG1wDhSs=; 7, 36 -- b=ELnHk9KlWP9I9T1RSK8vnrxBal4S5WvviLAtPk/wyeYfd8TtnzLbA+Ldi2tulKqvEP 7, 36 -- UnRkMaT0+OSBzXdlqLEkK4NvByAoImW/0HowbGzOkcpBOiJuXML4FplbYW6RArhdAUBw 7, 36 -- Lux2MK+vf/bMgHwiialf4akpAypdJYa4FchAQLxrx4hcGYj0+PQGiDWw/zGpjfwQa+63 7, 36 -- q2fbS0wAwwTuf4uv/Q3J87+OIXmauleEY8ab1TM4UU+TA8yaFAJd6sq25+5GZCn9mnaD 7, 36 -- a+P0m3bSCo0ZdnH2oWES2CrQuNeoeTkJqIAB9qcR15JGodzceXX//CKq+vM1rT89+Jhm 7, 36 -- Vn2g== 7, 36 -- X-Received: by 10.42.151.67 with SMTP id d3mr1178748icw.56.1422412470714; 7, 36 -- Tue, 27 Jan 2015 18:34:30 -0800 (PST) 7, 36 -- Received: from [192.168.1.126] (static-50-53-94-149.bvtn.or.frontiernet.net. [50.53.94.149]) 7, 36 -- by mx.google.com with ESMTPSA id t5sm8733284ign.12.2015.01.27.18.34.29 7, 36 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 7, 36 -- Tue, 27 Jan 2015 18:34:30 -0800 (PST) 7, 36 -- Content-Type: text/plain; charset=utf-8 7, 36 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.4\)) 7, 36 -- Subject: Re: [Microscopy] viaWWW:Spot microscope camera - help needed 7, 36 -- From: Jerry Biehler {jerry.biehler-at-gmail.com} 7, 36 -- In-Reply-To: {201501280221.t0S2LeE8002157-at-ns.microscopy.com} 7, 36 -- Date: Tue, 27 Jan 2015 18:34:28 -0800 7, 36 -- Cc: neurostar-at-outlook.it 7, 36 -- Message-Id: {CE776AD7-7A17-45CD-939C-E5223A4FCCC7-at-gmail.com} 7, 36 -- References: {201501280221.t0S2LeE8002157-at-ns.microscopy.com} 7, 36 -- To: microscopy-at-microscopy.com 7, 36 -- X-Mailer: Apple Mail (2.2070.4) 7, 36 -- Content-Transfer-Encoding: 8bit 7, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0S2YV8v002997 ==============================End of - Headers==============================
First, thanks to everyone for all the excellent advice I've received in the past on this list. I've got the rather unique task of running an SEM program accessible to the general public at a makerspace in Chicago, as a volunteer with no formal training and a shoestring budget. As far as I know, our circumstance is rather unique. There's no way I could have managed this long without all the help I've received here.
I managed to track down and repair the problem with wildly fluctuating vacuum readings (an extremely dirty Penning gauge) and the reason the vacuum was really bad after I fixed it (a leaking air admit solenoid.) But a bad shaft coupling on our Edwards E2M12 rotary pump was causing it to overheat and burn its oil, and, unfortunately, splattering said burnt oil up the rubber vacuum hose. I secured funding to get the pump overhauled, but now I need to know what to do with the hose. I figure it needs to be replaced, seeing as it reeks of burnt oil.
What I'm measuring seems to be about 7/8 I.D. and 1/4" wall thickness, so about 1 3/8" O.D. It's difficult to get a good reading as the hose ends were either clamped around something or stretched. Looking at http://www.pchemlabs.com/subcatagoryb.asp?pid=Pure-Gum-Rubber it looks like the gum rubber vacuum hose they sell jumps from 13/16" I.D. with 3/8" walls (listed as used for KF16) 1" with 1/2" walls (listed as used for KF25). The pump has a KF25 fitting, and the hose slips over a copper pipe in a concrete block as a vibration damper. But that size seems like it would be too large.
McMaster-Carr is local for us, so I checked http://www.mcmaster.com/#abrasion-resistant-gum-rubber-tubing/ and see vacuum rated red and tan tubing. (I have no idea what the color means. For reference, the tubing that came with the SEM was black.) The tan jumps from 3/4" with 5/8" walls to 1" with 1/2" walls. The red does come in 7/8" but it has 1/2" walls too. I'm not even sure if that would fit out the opening in the back of the SEM.
There's a good chance the vacuum hose is original to the scope, which is a Leica S430. Any help would be appreciated. Alternately, am I incorrect in assuming the hose should be replaced?
Thanks, Ryan
==============================Original Headers============================== 6, 16 -- From rdpierce-at-pobox.com Tue Jan 27 23:27:47 2015 6, 16 -- Received: from elna.mackenziegems.com (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S5RkTw024307 6, 16 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 23:27:46 -0600 6, 16 -- Received: from [127.0.0.1] (localhost [127.0.0.1]) 6, 16 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id 38A576E0A42 6, 16 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 23:27:44 -0600 (CST) 6, 16 -- Message-ID: {54C8734F.2000009-at-pobox.com} 6, 16 -- Date: Tue, 27 Jan 2015 23:27:43 -0600 6, 16 -- From: Ryan Pierce {rdpierce-at-pobox.com} 6, 16 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 6, 16 -- MIME-Version: 1.0 6, 16 -- To: microscopy-at-microscopy.com 6, 16 -- Subject: SEM: Vacuum hose source? 6, 16 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
good "standard" supplier is http://duniway.com/catalog/su-tubing-clamps.php (many components, not just the hose)
a cheap-cheap option is more work but you can make whatever diameter hose plus it can be easily compressed around a (much) smaller diameter tube since support spring is separate. Components: a) braided PVC of suitable diameter, either Home Depot etc. or McMaster Carr; b) continuous spring for supporting hose from collapsing, Again McMaster Carr.
tubing: http://www.mcmaster.com/#standard-high-pressure-pvc-tubing/=vnotog then select "clear" or from home page enter "High-Pressure PVC Tubing" in search line, then select "clear"
spring: http://www.mcmaster.com/#cut-to-length-springs/=vnp0wo then select "extension spring" or from home page enter "Cut-to-Length Extension Springs" stainless springs softer and won't rust. They sold in 20" pieces and will easily extend 10+ times. Springs made with wire 0.04" to 0.08" thick are best for diameters around 1".
3/4" to 1" ID vac. hose made in this fashion costs between $2 and $5 per foot depending on source of materials. It is transparent so oil accumulation can be seen before problems begin plus PVC will outlast rubber hands down. The oldest still in use I installed in 1998.
Use standard common hose-clams as long as they flat and at least close to 1/2" wide. Wire clamps not recommended.
Thin-wall-soft-not-braided low pressure PVC is even cheaper but less durable and unforgiving wrt vac. leaks at installation. Same longevity unless stressed.
For long straight connections use large diameter thin-wall copper tube, again from Home Depot, just ensure adequate cross-section for very long connections.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 1/28/2015 12:29 AM, rdpierce-at-pobox.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } First, thanks to everyone for all the excellent advice I've received in } the past on this list. I've got the rather unique task of running an SEM } program accessible to the general public at a makerspace in Chicago, as } a volunteer with no formal training and a shoestring budget. As far as I } know, our circumstance is rather unique. There's no way I could have } managed this long without all the help I've received here. } } I managed to track down and repair the problem with wildly fluctuating } vacuum readings (an extremely dirty Penning gauge) and the reason the } vacuum was really bad after I fixed it (a leaking air admit solenoid.) } But a bad shaft coupling on our Edwards E2M12 rotary pump was causing it } to overheat and burn its oil, and, unfortunately, splattering said burnt } oil up the rubber vacuum hose. I secured funding to get the pump } overhauled, but now I need to know what to do with the hose. I figure it } needs to be replaced, seeing as it reeks of burnt oil. } } What I'm measuring seems to be about 7/8 I.D. and 1/4" wall thickness, } so about 1 3/8" O.D. It's difficult to get a good reading as the hose } ends were either clamped around something or stretched. Looking at } http://www.pchemlabs.com/subcatagoryb.asp?pid=Pure-Gum-Rubber it looks } like the gum rubber vacuum hose they sell jumps from 13/16" I.D. with } 3/8" walls (listed as used for KF16) 1" with 1/2" walls (listed as used } for KF25). The pump has a KF25 fitting, and the hose slips over a copper } pipe in a concrete block as a vibration damper. But that size seems like } it would be too large. } } McMaster-Carr is local for us, so I checked } http://www.mcmaster.com/#abrasion-resistant-gum-rubber-tubing/ and see } vacuum rated red and tan tubing. (I have no idea what the color means. } For reference, the tubing that came with the SEM was black.) The tan } jumps from 3/4" with 5/8" walls to 1" with 1/2" walls. The red does come } in 7/8" but it has 1/2" walls too. I'm not even sure if that would fit } out the opening in the back of the SEM. } } There's a good chance the vacuum hose is original to the scope, which is } a Leica S430. Any help would be appreciated. Alternately, am I incorrect } in assuming the hose should be replaced? } } Thanks, } Ryan } } ==============================Original Headers============================== } 6, 16 -- From rdpierce-at-pobox.com Tue Jan 27 23:27:47 2015 } 6, 16 -- Received: from elna.mackenziegems.com (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) } 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S5RkTw024307 } 6, 16 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 23:27:46 -0600 } 6, 16 -- Received: from [127.0.0.1] (localhost [127.0.0.1]) } 6, 16 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id 38A576E0A42 } 6, 16 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2015 23:27:44 -0600 (CST) } 6, 16 -- Message-ID: {54C8734F.2000009-at-pobox.com} } 6, 16 -- Date: Tue, 27 Jan 2015 23:27:43 -0600 } 6, 16 -- From: Ryan Pierce {rdpierce-at-pobox.com} } 6, 16 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:31.0) Gecko/20100101 Thunderbird/31.3.0 } 6, 16 -- MIME-Version: 1.0 } 6, 16 -- To: microscopy-at-microscopy.com } 6, 16 -- Subject: SEM: Vacuum hose source? } 6, 16 -- Content-Type: text/plain; charset=utf-8; format=flowed } 6, 16 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
On Jan 27, 2015, at 9:43 PM, rdpierce-at-pobox.com wrote:
} First, thanks to everyone for all the excellent advice I've received } in } the past on this list. I've got the rather unique task of running an } SEM } program accessible to the general public at a makerspace in Chicago, } as } a volunteer with no formal training and a shoestring budget. As far } as I } know, our circumstance is rather unique. There's no way I could have } managed this long without all the help I've received here. } } I managed to track down and repair the problem with wildly fluctuating } vacuum readings (an extremely dirty Penning gauge) and the reason the } vacuum was really bad after I fixed it (a leaking air admit solenoid.) } But a bad shaft coupling on our Edwards E2M12 rotary pump was } causing it } to overheat and burn its oil, and, unfortunately, splattering said } burnt } oil up the rubber vacuum hose. I secured funding to get the pump } overhauled, but now I need to know what to do with the hose. I } figure it } needs to be replaced, seeing as it reeks of burnt oil. } } What I'm measuring seems to be about 7/8 I.D. and 1/4" wall thickness, } so about 1 3/8" O.D. It's difficult to get a good reading as the hose } ends were either clamped around something or stretched. Looking at } http://www.pchemlabs.com/subcatagoryb.asp?pid=Pure-Gum-Rubber it looks } like the gum rubber vacuum hose they sell jumps from 13/16" I.D. with } 3/8" walls (listed as used for KF16) 1" with 1/2" walls (listed as } used } for KF25). The pump has a KF25 fitting, and the hose slips over a } copper } pipe in a concrete block as a vibration damper. But that size seems } like } it would be too large. } } McMaster-Carr is local for us, so I checked } http://www.mcmaster.com/#abrasion-resistant-gum-rubber-tubing/ and see } vacuum rated red and tan tubing. (I have no idea what the color means. } For reference, the tubing that came with the SEM was black.) The tan } jumps from 3/4" with 5/8" walls to 1" with 1/2" walls. The red does } come } in 7/8" but it has 1/2" walls too. I'm not even sure if that would fit } out the opening in the back of the SEM. } } There's a good chance the vacuum hose is original to the scope, } which is } a Leica S430. Any help would be appreciated. Alternately, am I } incorrect } in assuming the hose should be replaced? } } Thanks, } Ryan
Dear Ryan, If you make a cut through the tubing, you should be able to measure ID and OD accurately. Yours, Bill
==============================Original Headers============================== 8, 35 -- From wtivol-at-sbcglobal.net Wed Jan 28 02:14:26 2015 8, 35 -- Received: from nm22-vm6.access.bullet.mail.gq1.yahoo.com (nm22-vm6.access.bullet.mail.gq1.yahoo.com [216.39.63.170]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0S8EQKS001410 8, 35 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2015 02:14:26 -0600 8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1422432865; bh=XSzdTAvSYETMXTQ53+oUZDvC9ZbjugpUTZz3OgZm7Yo=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=BOlRe1orBdvJoLk327YPQGl57UODLpHKdmgYHPGf7wmUJRMr52Nvh9BAtSSLzpyEYYdVwCiFJbdCzoleNB54I0AnJg5YCngi5zoVy2v0a2S06OeANA2HxDAiom+05ksqvQk0mStCtxq2FyUpboKNquZEBaYNTPcK7tUchXXDZ9qpE/i3FcpqpZyxMCgY3IF8bcljYBo+uUhpcntTzDkoRDpHAMpFUvGmYZxyFUnTCnH00XsSKDBu9jDnGl1YbZCyEzPytNxj2v43cHxe6iTl0dtr/SjQSX4Jk394aBieFqrKojbw+MjmDkg9dbvKWnBEJnXnc+Iti/hh2fRiAxCWPQ== 8, 35 -- Received: from [216.39.60.166] by nm22.access.bullet.mail.gq1.yahoo.com with NNFMP; 28 Jan 2015 08:14:25 -0000 8, 35 -- Received: from [67.195.22.116] by tm2.access.bullet.mail.gq1.yahoo.com with NNFMP; 28 Jan 2015 08:14:25 -0000 8, 35 -- Received: from [127.0.0.1] by smtp111.sbc.mail.gq1.yahoo.com with NNFMP; 28 Jan 2015 08:14:25 -0000 8, 35 -- X-Yahoo-Newman-Id: 961722.18361.bm-at-smtp111.sbc.mail.gq1.yahoo.com 8, 35 -- X-Yahoo-Newman-Property: ymail-3 8, 35 -- X-YMail-OSG: uAw2yy0VM1kvy.V8wNyegRcz8oKO6QeJNCxCh8pBrV3.nZQ 8, 35 -- IHwWMCuJRbPrRbCxMg1xvVQIbSnH255HrAU.ySf8uyhIRpqAsTpb7iiclaxI 8, 35 -- oveBvtBY3lq7dB2BN4LMRFx9Ex.nKng8mLtanNyuP1bKC9HJr15G.8ZGiczi 8, 35 -- lncII7JxafjOKowUsug3Xfrh9GbTLySzKK3ZgFFWhZNXn8PAHHxbhpE2vJTb 8, 35 -- 0EQzqnXMmmIxIQcQxL5veHUBxewbwU3Cm5dzwz0uHtd41UeZ9BBtdrm.msFN 8, 35 -- QiUI8xlZ9u1y9Y18je9sWifDZeAN2G56jJ1BgNhkFlw1R.qDQbCrIbM6j_eW 8, 35 -- I3InAvv0k6p2TW8JTGPGwaxlgt8R2zOZBdFoRgYfUjfErwmHRGtEZnC_InP6 8, 35 -- eMOwTmPu8FWaJl5Ta_GK321M3.cXDq2IqvIbA8wuWhoT1dl0V_n1zsLCk_ER 8, 35 -- FtW4nRSqQG2VYp52TC42qCBeyxnZiBY9Kn4xriLnySZnon6PuMovrv7Z4piO 8, 35 -- Srf2arjBKvK5G4S86fzcyKNafbsgjG.zYUoZ3Bpk0CcOI6h7Fmjt_sWKlILK 8, 35 -- Rz5uRrHDwL16jo2nyrwOCsHZlg.HKWtC2Vn6vuCndCiF_ZUQ1wFhoHbHl2pY 8, 35 -- MwMHNA5rGoeRQm.m3Fn7f6muZSNL1ae2pHM4_XZdWj2EBiOuKjRcD59KGhmo 8, 35 -- vdhUg1AdL.YlGVzzYmOPPvrGIP4kanA-- 8, 35 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 8, 35 -- Message-Id: {84196C3C-87E7-4830-B1E7-3F5EF198B635-at-sbcglobal.net} 8, 35 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 8, 35 -- To: microscopy-at-microscopy.com 8, 35 -- In-Reply-To: {201501280543.t0S5hjYw006423-at-ns.microscopy.com} 8, 35 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 35 -- Content-Transfer-Encoding: 7bit 8, 35 -- Mime-Version: 1.0 (Apple Message framework v936) 8, 35 -- Subject: Re: [Microscopy] SEM: Vacuum hose source? 8, 35 -- Date: Wed, 28 Jan 2015 00:14:23 -0800 8, 35 -- References: {201501280543.t0S5hjYw006423-at-ns.microscopy.com} 8, 35 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Virginia Tech is seeking an Instrument specialist for its Nanoscale Characterization and Fabrication Laboratory. The selected candidate will: Operate instrumentation in the Nanoscale Characterization and Fabrication Laboratory. Maintain and operate the FEI Helios Nanolab 600 Focused Ion Beam (FIB) in the NCFL. Keep the laboratory up to date in the methods and techniques used with these instruments. Perform FIB analyses and provide assessments of the results, support individual research with training on the instruments and guidance on the techniques. Provide analytical service to industrial clients. Maintain logs of all equipment usage and provide info to accounting monthly for timely billing through the service center. Comply with all Environmental Health and Safety regulations for proper lab operation. Keep up to date in the field on current techniques and procedures. Provide demonstrations for classes and visitors, and assistance on other instruments as needed. Virginia Tech is an Equal Opportunity/Affirmative Action Institution.
For further information or to apply, please go to: https://listings.jobs.vt.edu/postings/54282
Thank you, Christopher Winkler, PhD Senior Research Associate Nanoscale Characterization and Fabrication Laboratory Virginia Tech
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Message: Does anyone have a manual for a JEOL hollow-cone diffraction unit? Specifically, it's labeled HCID 10 and is part of a JEOL 2010 TEM. Thanks in advance!
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Title-Subject: [Filtered] SAVE THE DATE: NESM's Annual Spring Meeting -at- Bruker!
Message: Dear fellow microscopists,
This is a reminder to SAVE THE DATE for the New England Society for Microscopy’s annual Spring Meeting, which will take place on Thursday, March 5th at Bruker Corporation in Billerica, MA. The meeting will consist of facility tours, a buffet dinner and two technical talks showcasing microscopy application and innovation in the materials and life sciences. Stay tuned – more information, including speaker abstracts and bios, will follow in the coming weeks.
We hope to see you in March!
Cheers, NESM Board
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Email: sniper711220-at-hotmail.com Name: Gary
Organization: University of Oxford
Title-Subject: [Filtered] Analysis of Pb in the SEM-EDS
Message: Hi I have several copper-tin-lead alloy standards with varying Pb contents(5, 10, 15, 20). I analyzed them by the SEM-EDS, but it keeps giving me lower Pb concentrations(around 2, 5, 7, 10 individually). The totals are good around 100 precents, but just inaccurate(tin is the same, bur Pb is lower and Cu is higher than their real values. I use the default internal calibration (leaded glass) to calibrate Pb element. It is known that Pb in the copper alloy will form a separate phase.
Have anyone encountered such a problem and how to solve it ？
Thanks Gary
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 30 07:02:53 2015 15, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0UD2rlK014299 15, 17 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2015 07:02:53 -0600 15, 17 -- Message-ID: {54CB80FD.8000304-at-microscopy.com} 15, 17 -- Date: Fri, 30 Jan 2015 07:02:53 -0600 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:Analysis of Pb in the SEM-EDS 15, 17 -- References: {201501300509.t0U59pqm004683-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201501300509.t0U59pqm004683-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201501300509.t0U59pqm004683-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
How flat is the surface? What is the phase distribution on a micro-scale? If the Pb is present as a second phase it might well be preferentially polished away changing the volume (and mass) fraction and skewing the results.
Also, EDS correction routines are written to process intensities from homogeneous analysis volumes. If you are trying to lower the magnification and scan an area, you will present the sum of the phase intensities to the analyzer. However, Pb probably had nothing to do in determining the interaction volume in the regions where the Cu and Sn are nor did the Cu and Sn x-rays undergo absorption by Pb atoms. Once in a while, EDS might give the right answer in such cases, but I would not count on it being the right.
BTW, the same issue would be true of WDS too. The matrix corrections are much the same. They assume a homogenous sample volume.
Warren
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, January 30, 2015 7:04 AM To: Straszheim, Warren E [BIOTC]
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Email: sniper711220-at-hotmail.com Name: Gary
Organization: University of Oxford
Title-Subject: [Filtered] Analysis of Pb in the SEM-EDS
Message: Hi I have several copper-tin-lead alloy standards with varying Pb contents(5, 10, 15, 20). I analyzed them by the SEM-EDS, but it keeps giving me lower Pb concentrations(around 2, 5, 7, 10 individually). The totals are good around 100 precents, but just inaccurate(tin is the same, bur Pb is lower and Cu is higher than their real values. I use the default internal calibration (leaded glass) to calibrate Pb element. It is known that Pb in the copper alloy will form a separate phase.
Have anyone encountered such a problem and how to solve it ？
Thanks Gary
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==============================Original Headers============================== 23, 47 -- From wesaia-at-iastate.edu Fri Jan 30 09:03:49 2015 23, 47 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2on0084.outbound.protection.outlook.com [65.55.169.84]) 23, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0UF3mSA003300 23, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Jan 2015 09:03:48 -0600 23, 47 -- Received: from BN1PR04MB551.namprd04.prod.outlook.com (10.141.65.148) by 23, 47 -- BN1PR04MB185.namprd04.prod.outlook.com (10.255.204.153) with Microsoft SMTP 23, 47 -- Server (TLS) id 15.1.75.20; Fri, 30 Jan 2015 15:03:43 +0000 23, 47 -- Received: from BN1PR04MB552.namprd04.prod.outlook.com (10.141.65.152) by 23, 47 -- BN1PR04MB551.namprd04.prod.outlook.com (10.141.65.148) with Microsoft SMTP 23, 47 -- Server (TLS) id 15.1.65.19; Fri, 30 Jan 2015 15:03:41 +0000 23, 47 -- Received: from BN1PR04MB552.namprd04.prod.outlook.com ([169.254.6.42]) by 23, 47 -- BN1PR04MB552.namprd04.prod.outlook.com ([169.254.6.53]) with mapi id 23, 47 -- 15.01.0065.013; Fri, 30 Jan 2015 15:03:41 +0000 23, 47 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 23, 47 -- To: "sniper711220-at-hotmail.com" {sniper711220-at-hotmail.com} 23, 47 -- CC: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 23, 47 -- Subject: RE: [Microscopy] viaWWW:Analysis of Pb in the SEM-EDS 23, 47 -- Thread-Topic: [Microscopy] viaWWW:Analysis of Pb in the SEM-EDS 23, 47 -- Thread-Index: AQHQPI1fprqTSG+pr02aw+LOK7/ARpzYwKsA 23, 47 -- Date: Fri, 30 Jan 2015 15:03:41 +0000 23, 47 -- Message-ID: {BN1PR04MB552DFACAA17225677F53FDCD7310-at-BN1PR04MB552.namprd04.prod.outlook.com} 23, 47 -- References: {201501301304.t0UD4J7D015082-at-ns.microscopy.com} 23, 47 -- In-Reply-To: {201501301304.t0UD4J7D015082-at-ns.microscopy.com} 23, 47 -- Accept-Language: en-US 23, 47 -- Content-Language: en-US 23, 47 -- X-MS-Has-Attach: 23, 47 -- X-MS-TNEF-Correlator: 23, 47 -- x-originating-ip: [129.186.227.4] 23, 47 -- authentication-results: hotmail.com; dkim=none (message not signed) 23, 47 -- header.d=none;hotmail.com; dmarc=none action=none header.from=iastate.edu; 23, 47 -- x-dmarcaction-test: None 23, 47 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID:(3005004);SRVR:BN1PR04MB551;UriScan:; 23, 47 -- x-exchange-antispam-report-test: UriScan:; 23, 47 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:;SRVR:BN1PR04MB551; 23, 47 -- x-forefront-prvs: 04724A515E 23, 47 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(13464003)(377454003)(87936001)(54606007)(40100003)(575784001)(86362001)(2656002)(325944007)(77156002)(62966003)(90282001)(122556002)(102836002)(15975445007)(2950100001)(54356999)(50986999)(76176999)(2501002)(33656002)(74316001)(75432002)(92566002)(64544003)(89122001)(110136001)(2900100001)(46102003)(54206007)(88552001)(19580395003)(76576001)(1720100001)(106116001)(66066001)(551984002)(2351001)(19580405001);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR04MB551;H:BN1PR04MB552.namprd04.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 23, 47 -- Content-Type: text/plain; charset="us-ascii" 23, 47 -- MIME-Version: 1.0 23, 47 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 30 Jan 2015 15:03:41.1922 23, 47 -- (UTC) 23, 47 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 23, 47 -- X-MS-Exchange-CrossTenant-id: 0347d89a-0174-4dd3-adeb-3339c89c35f5 23, 47 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN1PR04MB551 23, 47 -- X-Microsoft-Antispam: BCL:0;PCL:0;RULEID:;SRVR:BN1PR04MB185; 23, 47 -- X-OriginatorOrg: iastate.edu 23, 47 -- Content-Transfer-Encoding: 8bit 23, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t0UF3mSA003300 ==============================End of - Headers==============================
I would not use the Pb in glass standard for this analysis. You should try to use a standard as close to your unknown composition. Pb in SiO2 is not close. I would try using one of you CuSnPb standards as the standard and see how the results turn out. You say you 'use the default internal calibration (leaded glass) to calibrate Pb element', did you use the same operating conditions? kV, take off angle. Also which lines for each element did you use? K L M? Be careful of background too.
wesaia-at-iastate.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } How flat is the surface? What is the phase distribution on a micro-scale? If the Pb is present as a second phase it might well be preferentially polished away changing the volume (and mass) fraction and skewing the results. } } Also, EDS correction routines are written to process intensities from homogeneous analysis volumes. If you are trying to lower the magnification and scan an area, you will present the sum of the phase intensities to the analyzer. However, Pb probably had nothing to do in determining the interaction volume in the regions where the Cu and Sn are nor did the Cu and Sn x-rays undergo absorption by Pb atoms. Once in a while, EDS might give the right answer in such cases, but I would not count on it being the right. } } BTW, the same issue would be true of WDS too. The matrix corrections are much the same. They assume a homogenous sample volume. } } Warren } } -----Original Message----- } X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Friday, January 30, 2015 7:04 AM } To: Straszheim, Warren E [BIOTC] } Subject: [Microscopy] viaWWW:Analysis of Pb in the SEM-EDS } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: sniper711220-at-hotmail.com } } } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both sniper711220-at-hotmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: sniper711220-at-hotmail.com } Name: Gary } } Organization: University of Oxford } } Title-Subject: [Filtered] Analysis of Pb in the SEM-EDS } } Message: Hi } I have several copper-tin-lead alloy standards with varying Pb contents(5, 10, 15, 20). } I analyzed them by the SEM-EDS, but it keeps giving me lower Pb concentrations(around 2, 5, 7, 10 individually). } The totals are good around 100 precents, but just inaccurate(tin is the same, bur Pb is lower and Cu is higher than their real values. } I use the default internal calibration (leaded glass) to calibrate Pb element. } It is known that Pb in the copper alloy will form a separate phase. } } Have anyone encountered such a problem and how to solve it ？ } } Thanks } Gary } } Login Host: 163.1.130.189 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } -- } =========================================== } Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. 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Message: I am looking to purchase a used Si(Li) EDS system for a JEOL 2010 (horizontal EDS port). It seems the vendors have abandoned this technology for the SDD, but these systems are beyond our budget justification for such an old scope without STEM. If you are interested in selling such a system, please contact me offline.
Rich
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==============================Original Headers============================== 12, 17 -- From microscopylistserver-noreply-at-microscopy.com Sun Feb 1 12:52:20 2015 12, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t11IqKWm031166 12, 17 -- for {microscopy-at-microscopy.com} ; Sun, 1 Feb 2015 12:52:20 -0600 12, 17 -- Message-ID: {54CE75E4.2090502-at-microscopy.com} 12, 17 -- Date: Sun, 01 Feb 2015 12:52:20 -0600 12, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 12, 17 -- MIME-Version: 1.0 12, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 17 -- Subject: viaWWW:Looking for a used EDS system 12, 17 -- References: {201502011739.t11HdYp2030471-at-ns.microscopy.com} 12, 17 -- In-Reply-To: {201502011739.t11HdYp2030471-at-ns.microscopy.com} 12, 17 -- X-Forwarded-Message-Id: {201502011739.t11HdYp2030471-at-ns.microscopy.com} 12, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: lamiller-at-illinois.edu Name: Lou Ann Miller
Organization: Materials Research Laboratory, University of Illinois
Title-Subject: [Filtered] Job opening for an experienced Electron Microscopist
Message: Research Scientist(s) position Please see the link below for the job information and to apply electronically online:
*Note for those unfamiliar with University jobs here, a common question: Is the job for only one year with the "12 month appointment"?
All academic positions at the University are appointed on a 9- or 12-month service basis, eligible for annual renewal based upon mutual agreement and the annual performance review process. This Academic Professional position is a regularly recurring staff scientist role not subject to grant funding. See http://ap.illinois.edu for more information.
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230
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==============================Original Headers============================== 23, 18 -- From microscopylistserver-noreply-at-microscopy.com Mon Feb 2 08:20:04 2015 23, 18 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 23, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t12EK3Qv002907 23, 18 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2015 08:20:04 -0600 23, 18 -- Message-ID: {54CF8793.2000503-at-microscopy.com} 23, 18 -- Date: Mon, 02 Feb 2015 08:20:03 -0600 23, 18 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 18 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 23, 18 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 23, 18 -- MIME-Version: 1.0 23, 18 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 23, 18 -- Subject: viaWWW:Job opening for an experienced Electron Microscopist - Univ. 23, 18 -- of Illinois 23, 18 -- References: {201502021411.t12EBfYo002745-at-ns.microscopy.com} 23, 18 -- In-Reply-To: {201502021411.t12EBfYo002745-at-ns.microscopy.com} 23, 18 -- X-Forwarded-Message-Id: {201502021411.t12EBfYo002745-at-ns.microscopy.com} 23, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 23, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I had several questions from folks on the list serve about another job posting. I did not see it yesterday, but it appears to be a resend of the … maybe now 15-16 month ago email that somehow got retransmitted a while back.
I’m sorry, I do not know where in cyber this came from, but there is no TEM job opening at MRL laboratory at this time. ( They updated our Lync system, perhaps it was somehow caught in that action)
Thanks to those who asked, so I became aware, and many apologies for those looking for a job, that this has resurfaced. We filled both spots a year ago.
Lou Ann
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230
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Email: lou.howell-at-live.com Name: Louise
Organization: Institute of Cancer Research
Title-Subject: [Filtered] advice on new camera for Zeiss Axioplan 2
Message: Dear all,
IÂ’m looking into upgrading some software for my Zeiss Axioplan 2 microscope (currently have very old SmartCapture software). We look at protein expression levels and also DNA damage foci and so something which could quantify these signals would be ideal. Also IÂ’m looking into buying a new camera compatible with the new software/microscope.
Any advice on the best camera and suitable software compatible with this microscope would be most gratefully received.
Thank you very much in advance.
Louise.
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: KSU
Title-Subject: [Filtered] Calibration of Oxford EDS.
Message: Hi, I have EDS analysis of Au Nanoparticle on Copper TEM grid using Oxford EDS.But I am getting lot of cu peaks and instead of Au peak. I am using INCA microanalysos suit version 4.15. Could anybody suggest me how to do calibration and What kind of std reference sample needed for calibration.?
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 4 16:56:49 2015 14, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t14MunNp028822 14, 17 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2015 16:56:49 -0600 14, 17 -- Message-ID: {54D2A3B1.8020804-at-microscopy.com} 14, 17 -- Date: Wed, 04 Feb 2015 16:56:49 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:Calibration of Oxford EDS. 14, 17 -- References: {201502042042.t14Kg36Z024862-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201502042042.t14Kg36Z024862-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201502042042.t14Kg36Z024862-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You are always going to see Cu under those circumstances. The amount of Cu metal in the grid is vast compared to the size of an Au nanoparticle. As your electron beam passes through the Au nanoparticle, some electrons will scatter and hit the grid producing fluorescence. There can also be electrons scattered from electron microscope components such as apertures as well — though this is less a problem these days than it used to be (depends on the age of your microscope).
If you aren’t interested in Cu, I suggest you just ignore the peak. If you are interested in Cu in your nanoparticle, then producers such as EMS, Ted Pella and Ladd make a variety of grids out of a variety of elements and compounds so that you can almost certainly find some element you don’t care about.
When avoiding Cu, I’ve had luck using beryllium, and silicon nitride.
For general calibration, a simple and very common approach is to use the Cliff-Lorimer method of k-factors: Cliff, G., & Lorimer, G. W. (1975). The Quantitative Analysis of Thin Specimens. Journal of Microscopy, 103(2), 203–207.
The Oxford software has this method built in and allows you to define those k-factors once you measure them (using the method from the paper above). The Oxford software also has some good guesses built in if you don’t need very much accuracy.
I have also found it useful to extract peak areas from TEM/EDS spectra and process them myself. I first use fityk, Python, or the Oxford/Bruker softwares to fit peak areas. Then I use k-factors and apply a thickness correction using some software I wrote for myself (https://github.com/ZGainsforth/StoichiometryFitter/blob/master/Stoichiometry%20Fitter%20Screenshot.png) You might like a solution like this if you want to control every aspect of the analysis.
Hopefully that clears it up?
Zack
On Feb 4, 2015, at 3:14 PM, microscopylistserver-noreply-at-microscopy.com wrote:
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: KSU
Title-Subject: [Filtered] Calibration of Oxford EDS.
Message: Hi, I have EDS analysis of Au Nanoparticle on Copper TEM grid using Oxford EDS.But I am getting lot of cu peaks and instead of Au peak. I am using INCA microanalysos suit version 4.15. Could anybody suggest me how to do calibration and What kind of std reference sample needed for calibration.?
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==============================Original Headers============================== 32, 36 -- From zackg-at-berkeley.edu Wed Feb 4 17:43:44 2015 32, 36 -- Received: from mail-ie0-f169.google.com (mail-ie0-f169.google.com [209.85.223.169]) 32, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t14NhimD016685 32, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Feb 2015 17:43:44 -0600 32, 36 -- Received: by mail-ie0-f169.google.com with SMTP id rl12so6241285iec.0 32, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Feb 2015 15:43:43 -0800 (PST) 32, 36 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 32, 36 -- d=1e100.net; s=20130820; 32, 36 -- h=x-gm-message-state:from:content-type:content-transfer-encoding 32, 36 -- :subject:date:references:to:message-id:mime-version; 32, 36 -- bh=LJPiuRHVw6TQMIPgcC7byr6GC1byPI6/WZX6dN9FCTs=; 32, 36 -- b=ZZLbGeCO83B/liSo0DeOY6iNPJG6AYJPREPpWf3SHKmD8KFFcT6fir+Es7aB0HKYLm 32, 36 -- KJh1QZIZdw1gXu2LY978BbzwmaFvQ2Q3y3Es0UK5T3SwmYumFYenZNCBKwhSLc8z3rhn 32, 36 -- IZrGNaEvtTqu77sV18ZzapK52JspKNW3YDkQTbTz5oO7sEFF6fEzTX5xNjuvGnFnIwPl 32, 36 -- NnpKEHz2+Zs68I3ZKclKAnAKfc/57xXSkJiVfofDSKTjLA5WLz1BjdmJlnL0iNhJXmh0 32, 36 -- oiBFGqUUTYJHIGJ0NlHRC8EKsDEVHxBinULp52wcabZl8qwwq/rzQZsenGRBX/xq0nDt 32, 36 -- D+yw== 32, 36 -- X-Gm-Message-State: ALoCoQk3T0SVLC6HSYF1/vqqGbMNpZ8lmzYNmmFl0TkXoN69/8mND2sk6zll5QjwS96w968kQwhq 32, 36 -- X-Received: by 10.50.154.106 with SMTP id vn10mr27241541igb.49.1423093423558; 32, 36 -- Wed, 04 Feb 2015 15:43:43 -0800 (PST) 32, 36 -- Received: from continuum.home (pool-173-60-44-43.lsanca.fios.verizon.net. [173.60.44.43]) 32, 36 -- by mx.google.com with ESMTPSA id g15sm1628049ioi.22.2015.02.04.15.43.42 32, 36 -- for {Microscopy-at-microscopy.com} 32, 36 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 32, 36 -- Wed, 04 Feb 2015 15:43:42 -0800 (PST) 32, 36 -- From: Zack Gainsforth {zackg-at-berkeley.edu} 32, 36 -- Content-Type: text/plain; charset=utf-8 32, 36 -- Subject: Re: [Microscopy] viaWWW:Calibration of Oxford EDS. 32, 36 -- Date: Wed, 4 Feb 2015 15:43:41 -0800 32, 36 -- References: {64508464-990E-4EDC-8AF5-7CD62986884F-at-berkeley.edu} 32, 36 -- To: Microscopy-at-microscopy.com 32, 36 -- Message-Id: {4F29FE4E-AE92-44C3-97F3-BCF086A09908-at-berkeley.edu} 32, 36 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.6\)) 32, 36 -- X-Mailer: Apple Mail (2.2070.6) 32, 36 -- Content-Transfer-Encoding: 8bit 32, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t14NhimD016685 ==============================End of - Headers==============================
From mrsivonneemile-at-gmail.com Sat Feb 7 12:28:05 2015 Return-Path: {mrsivonneemile-at-gmail.com} Received: from n10.c03.server-system.net (n10.c03.server-system.net [72.47.224.10]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t17IS5kS008220; Sat, 7 Feb 2015 12:28:05 -0600 Received: from [146.71.103.85] (port=51998 helo=User) by n10.c03.server-system.net with esmtpa (Exim 4.80.1) (envelope-from {mrsivonneemile-at-gmail.com} ) id 1YKA6r-0007G7-S4; Sat, 07 Feb 2015 10:28:01 -0800 Reply-To: {mrsivonnemile-at-religious.com}
X-from: rashmi_mehata-at-yahoo.com
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] 'FEI' TEM HRTEM ESEM service Manuals
Message: HI
Our 'FEI' TEM, HRTEM and ESEM are not functioning for some time. Does anyone have the service manuals for this microscopes which will help us bring back these instruments into working conditions
Regards Rashmi
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Email: ian.maclaren-at-glasgow.ac.uk Name: Ian MacLaren
Organization: University of Glasgow
Title-Subject: [Filtered] Two Postdoc Positions - Glasgow and Oxford
Message: SCHOOL OF PHYSICS AND ASTRONOMY, UNIVERSITY OF GLASGOW DEPARTMENT OF MATERIALS, UNIVERSITY OF OXFORD
Two post-doctoral research positions in fast pixelated detectors for scanning transmission electron microscopy
Oxford: Research Associate, Grade 7 / Salary in the range: £30,434 to £33,242 p.a. / Vacancy ID: 117029
Applications are invited for two fixed-term (up to 36 months) postdoctoral positions (one at Glasgow and one at Oxford) in research associated with using fast pixelated detectors in STEM.
The overarching aim of the project is to use fast pixelated detectors to record the intensity as a function of scattering angle in the detector plane of a STEM, which is effectively a diffraction pattern. By recording each two-dimensional diffraction pattern as a function of probe position in a two-dimensional scan, a four-dimensional data set can be recorded that is the ultimate STEM imaging experiment. Such a rich dataset contains information about the phase shift that results from transmission, about the composition of the sample, the strain in the sample and the three-dimensional ordering in the sample. We propose to develop the methods to record this 4D data set, using fast pixelated detectors, and by developing an optimised direct-detection system, together with the methods to process such datasets to enable physically useful measurements to be made. Application areas include imaging of soft materials, detection of fields and charge transfer, separation of strain and compositional information, and measurement of three-dimensional crystallographic ordering.
The posts have been created through the funding by the EPSRC of a joint project between The Universities of Glasgow and Oxford on developing methods and applications associated with pixelated detectors in aberration-corrected scanning transmission electron microscopy.
The posts are available from 1 April 2015. By the start date, applicants should have a good first degree and completed PhD in Physics, Materials, Engineering, Chemistry or a related field. They should have experience with operating a STEM and a good understanding of its principles of operation, a high level of expertise in the theory of electron scattering and image formation in the electron microscope, and excellent IT skills including the use of electron microscopy specific software packages and an ability to write code using a suitable high-level language.
The closing date for applications is 11 March 2015 (Glasgow, midnight; Oxford, midday) with interviews planned for 24 March 2015 (in Glasgow). It is important that candidates submit applications to both Oxford and Glasgow (these can be identical applications) if they wish to be considered for both posts.
Informal enquiries about the Glasgow post may be directed to Dr Ian MacLaren, ian.maclaren-at-glasgow.ac.uk Informal enquiries about the Oxford post should be directed to Prof. Peter Nellist, peter.nellist-at-materials.ox.ac.uk
Glasgow Application Details:
Apply online at www.glasgow.ac.uk/jobs
Closing date: 11 March 2015. The School of Physics and Astronomy has been awarded Juno Champion status and also the Athena SWAN Silver Award.
The University has recently been awarded the Athena SWAN Institutional Bronze Award.
The University is committed to equality of opportunity in employment.
The University of Glasgow, charity number SC004401.
Oxford Application Details:
Applications for the Oxford post are to be made online (vacancy reference 117029). To apply for this role and for further details, including the job description and selection criteria, please click on the link below:
The Department of Materials is an Athena Swan silver award holder. Applications are particularly welcome from women and black and ethnic minority candidates, who are under-represented in research posts in the Department.
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Hello Everyone, I'm looking for suggestions about a beam sensitive sample.
I'm cutting thin sections with a cryomicrotome of HIPS, High Impact Polystyrene, that is toughened with polybutadiene. I'm cutting at -100 C because of the low TG of the polybutadiene and staining with Os. My thin sections are very beam sensitive, too sensitive to focus well and get a good image. I'm imaging at 80KV with 8800X magnification. If I spread out my beam to reduce beam damage I can't see my sample to focus and any pictures I take look crappy.
Any suggestions?
I've been thinking about trying carbon coated grids to help act as a heat sink as well as going to a larger TEM spot setting. I have read that lower KV can make the damage worse because each electron resides in the thin section longer and produced more heat. Is this a tried and true observation?
Thanks!!!
Frank
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==============================Original Headers============================== 9, 28 -- From frank_karl-at-ardl.com Mon Feb 9 12:15:49 2015 9, 28 -- Received: from cal1-mh775.smtproutes.com (cal1-mh775.smtproutes.com [208.70.91.141]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t19IFmQm020125 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 12:15:48 -0600 9, 28 -- X-Katharion-ID: 1423505727.99310.cal1-mh775 9, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by 9, 28 -- cal1-mh775.smtproutes.com [(192.69.16.141)] with ESMTP via TCP 9, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 09 Feb 2015 18:15:27 +0000 9, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 9, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 9 9, 28 -- Feb 2015 13:15:26 -0500 9, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 9, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 9, 28 -- {microscopy-at-microscopy.com} 9, 28 -- Date: Mon, 9 Feb 2015 13:15:26 -0500 9, 28 -- Subject: TEM and HIPS 9, 28 -- Thread-Topic: TEM and HIPS 9, 28 -- Thread-Index: AdBElGBOLrir6G9eQt6ILmdqm8wZ1g== 9, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C83443E1B-at-exchange2k7.ad.ardl.com} 9, 28 -- Accept-Language: en-US 9, 28 -- Content-Language: en-US 9, 28 -- X-MS-Has-Attach: 9, 28 -- X-MS-TNEF-Correlator: 9, 28 -- acceptlanguage: en-US 9, 28 -- Content-Type: text/plain; charset="us-ascii" 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t19IFmQm020125 ==============================End of - Headers==============================
You'll probably have better luck if you can put a thin carbon coating on top of your microtomed sample prior to examination in the TEM. A nanometer or less--just a few seconds in most carbon coaters--should minimize the charging and preserve your samples under the beam. Staining should also help stabilize the sample but apparently not in this case.
If you don't have a coater then you can try "cooking" the microtome sections in the TEM: go to a few thousand magnification, remove the condenser aperture and adjust your condenser lenses to the largest spot size (highest beam current), and spread the beam out to illuminate a large fraction of the thin section. Leave it in this condition, assuming the sample is not sputtering away, for a few minutes. Take a look at 10kx magnification and see if it's stable. If not, try again for a longer time. This may never stabilize the section but often helps in my experience.
We look at HIPS samples (albiet stained samples with a few angstroms of carbon coating) all the time at 200kV and 300kV and even run long STEM maps without issue. Try a higher accelerating voltage if your microscope is capable of it. The damage mechanisms are complicated in TEM but generally lower voltages increase interaction cross-section of the electron in the sample, leading to higher sample temperatures and radiolysis effects. Higher accelerating voltages minimize the interaction cross-section but lead to knock-on damage. Egerton, Li, and Malac wrote a paper titled, "Radiation Damage in the TEM and SEM" (Micron 35 (2004) 399-409) which can be a good starting point for understanding beam induced damage.
Good luck, Chris Senior Research Associate Nanoscale Characterization and Fabrication Laboratory Institute for Critical Technology and Applied Science Virginia Tech (540) 200-9511
On Mon, Feb 9, 2015 at 1:25 PM, {frank_karl-at-ardl.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Everyone, } I'm looking for suggestions about a beam sensitive sample. } } I'm cutting thin sections with a cryomicrotome of HIPS, High Impact Polystyrene, that is toughened with polybutadiene. I'm cutting at -100 C because of the low TG of the polybutadiene and staining with Os. } My thin sections are very beam sensitive, too sensitive to focus well and get a good image. I'm imaging at 80KV with 8800X magnification. If I spread out my beam to reduce beam damage I can't see my sample to focus and any pictures I take look crappy. } } Any suggestions? } } I've been thinking about trying carbon coated grids to help act as a heat sink as well as going to a larger TEM spot setting. I have read that lower KV can make the damage worse because each electron resides in the thin section longer and produced more heat. Is this a tried and true observation? } } Thanks!!! } } Frank } } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } } ==============================Original Headers============================== } 9, 28 -- From frank_karl-at-ardl.com Mon Feb 9 12:15:49 2015 } 9, 28 -- Received: from cal1-mh775.smtproutes.com (cal1-mh775.smtproutes.com [208.70.91.141]) } 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t19IFmQm020125 } 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 12:15:48 -0600 } 9, 28 -- X-Katharion-ID: 1423505727.99310.cal1-mh775 } 9, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by } 9, 28 -- cal1-mh775.smtproutes.com [(192.69.16.141)] with ESMTP via TCP } 9, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 09 Feb 2015 18:15:27 +0000 } 9, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by } 9, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 9 } 9, 28 -- Feb 2015 13:15:26 -0500 } 9, 28 -- From: Frank Karl {frank_karl-at-ardl.com} } 9, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" } 9, 28 -- {microscopy-at-microscopy.com} } 9, 28 -- Date: Mon, 9 Feb 2015 13:15:26 -0500 } 9, 28 -- Subject: TEM and HIPS } 9, 28 -- Thread-Topic: TEM and HIPS } 9, 28 -- Thread-Index: AdBElGBOLrir6G9eQt6ILmdqm8wZ1g== } 9, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C83443E1B-at-exchange2k7.ad.ardl.com} } 9, 28 -- Accept-Language: en-US } 9, 28 -- Content-Language: en-US } 9, 28 -- X-MS-Has-Attach: } 9, 28 -- X-MS-TNEF-Correlator: } 9, 28 -- acceptlanguage: en-US } 9, 28 -- Content-Type: text/plain; charset="us-ascii" } 9, 28 -- MIME-Version: 1.0 } 9, 28 -- Content-Transfer-Encoding: 8bit } 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t19IFmQm020125 } ==============================End of - Headers==============================
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Yes to both your questions. Putting the sections on carbon-coated grids will help with heat dissipation, and lowering the kV will increase beam damage because of more beam interactions with your specimens. We typically use a 10 µm spot when looking at our thin sections, especially at lower mags. And spread the illumination. Maybe try 100 kV. But - how thick are your thin sections? If they're 100 - 120 nm, try cutting them 80 - 90 nm. Less beam energy deposition in the thinner sections. And of course, this being reality, if they're already this thin (or thinner), try 100 nm sections, as they'll be more robust, and maybe use the higher kV. Probably the carbon-coated grids will do the best, but changing kV is easy.
(Hey, it's all quantum - your sections are simultaneously in burst and unburst states, you just have to pick the ones that collapse to the unburst state when you observe them.)
Phil
} Hello Everyone, } I'm looking for suggestions about a beam sensitive sample. } } I'm cutting thin sections with a cryomicrotome of HIPS, High Impact Polystyrene, that is toughened with polybutadiene. I'm cutting at -100 C because of the low TG of the polybutadiene and staining with Os. } My thin sections are very beam sensitive, too sensitive to focus well and get a good image. I'm imaging at 80KV with 8800X magnification. If I spread out my beam to reduce beam damage I can't see my sample to focus and any pictures I take look crappy. } } Any suggestions? } } I've been thinking about trying carbon coated grids to help act as a heat sink as well as going to a larger TEM spot setting. I have read that lower KV can make the damage worse because each electron resides in the thin section longer and produced more heat. Is this a tried and true observation? } } Thanks!!! } } Frank } } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Mon Feb 9 12:48:03 2015 5, 32 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t19Ilvh6020004 5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 12:48:03 -0600 5, 32 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40] (may be forged)) 5, 32 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t19Ikoco029695; 5, 32 -- Mon, 9 Feb 2015 13:46:50 -0500 5, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 5, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.195.1; Mon, 9 Feb 2015 13:46:49 -0500 5, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.195.1; Mon, 9 Feb 2015 13:46:48 -0500 5, 32 -- Message-ID: {54D90098.1080308-at-cmich.edu} 5, 32 -- Date: Mon, 9 Feb 2015 13:46:48 -0500 5, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: {frank_karl-at-ardl.com} , micro {microscopy-at-microscopy.com} 5, 32 -- Subject: Re: [Microscopy] TEM and HIPS 5, 32 -- References: {201502091832.t19IWKIe004860-at-ns.microscopy.com} 5, 32 -- In-Reply-To: {201502091832.t19IWKIe004860-at-ns.microscopy.com} 5, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-Originating-IP: [141.209.2.100] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -0.40 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 05NOiKOIq - f51c085a9689 - 20150209 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Does anyone know of an anti-catalase primary antibody for post-label on HM20 sections? I have rabbit and mouse 2Ab. A researcher gave me the ABCAM anti-catalase antibody ab16731 but it was not tested for TEM and my results were negative.
Any suggestions or recommendations would be greatly appreciated.
Karen Kelley University of Florida
==============================Original Headers============================== 6, 40 -- From vau-at-ufl.edu Mon Feb 9 17:12:33 2015 6, 40 -- Received: from smtp.ufl.edu (smtp-prod04.osg.ufl.edu [128.227.74.220]) 6, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t19NCWHo018835 6, 40 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Feb 2015 17:12:32 -0600 6, 40 -- X-UFL-GatorLink-Authenticated: authenticated as () with from 10.241.106.146 6, 40 -- Received: from UFEXCH-CASHT02.ad.ufl.edu ([10.241.106.146]) 6, 40 -- by smtp.ufl.edu (8.14.4/8.14.4/3.0.0) with ESMTP id t19NCVAc019774 6, 40 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 6, 40 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Feb 2015 18:12:32 -0500 6, 40 -- Received: from UFEXCH-MBXN03.ad.ufl.edu (10.253.18.73) by 6, 40 -- UFEXCH-CASHT02.ad.ufl.edu (10.241.106.146) with Microsoft SMTP Server (TLS) 6, 40 -- id 14.3.181.6; Mon, 9 Feb 2015 18:12:32 -0500 6, 40 -- Received: from UFEXCH-MBXN02.ad.ufl.edu ([fe80::4801:abc9:e123:b1bb]) by 6, 40 -- UFEXCH-MBXN03.ad.ufl.edu ([fe80::e09b:9163:ed13:4711%18]) with mapi id 6, 40 -- 14.03.0181.006; Mon, 9 Feb 2015 18:12:31 -0500 6, 40 -- From: "Kelley,Karen L" {vau-at-ufl.edu} 6, 40 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 6, 40 -- Subject: anti-catalase 1Ab for TEM 6, 40 -- Thread-Topic: anti-catalase 1Ab for TEM 6, 40 -- Thread-Index: AQHQRL3hebxG7gANkUGY2qGV92XAVA== 6, 40 -- Date: Mon, 9 Feb 2015 23:12:30 +0000 6, 40 -- Message-ID: {D0FEA918.FFCF%vau-at-ufl.edu} 6, 40 -- Accept-Language: en-US 6, 40 -- Content-Language: en-US 6, 40 -- X-MS-Has-Attach: 6, 40 -- X-MS-TNEF-Correlator: 6, 40 -- user-agent: Microsoft-MacOutlook/14.0.0.100825 6, 40 -- x-originating-ip: [10.36.9.36] 6, 40 -- Content-Type: text/plain; charset="us-ascii" 6, 40 -- Content-ID: {C1D377045D81BC4797555A963C45145C-at-ad.ufl.edu} 6, 40 -- MIME-Version: 1.0 6, 40 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.13.68,1.0.33,0.0.0000 6, 40 -- definitions=2015-02-09_04:2015-02-09,2015-02-09,1970-01-01 signatures=0 6, 40 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 6, 40 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 6, 40 -- engine=7.0.1-1402240000 definitions=main-1502090228 6, 40 -- X-Spam-Level: * 6, 40 -- X-UFL-Spam-Level: * 6, 40 -- Content-Transfer-Encoding: 8bit 6, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t19NCWHo018835 ==============================End of - Headers==============================
it may be that it is not the primary that is not working. Do you have positive controls with the secondaries? Catalase is usually so abundant and some molecules would always survive in my opinion, certainly in HM20 preps. Feel free to contact me or Peter van de Plas (email below) off-list if you would like some ideas on how to take this further.
Thank you and good luck,
Jan Leunissen Aurion
Aurion ImmunoGold Reagents Binnenhaven 5 6709 PD Wageningen The Netherlands
I looked up this antibody. It might be helpful if you can share the protocol you used for sample preparation and immunolabeling. Feel free to call or email. Thanks.
Hong Emory EM 404 712 8491 hyi-at-emory.edu
On 2/9/15 6:16 PM, "vau-at-ufl.edu" {vau-at-ufl.edu} wrote:
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==============================Original Headers============================== 11, 41 -- From hyi-at-emory.edu Mon Feb 9 21:41:30 2015 11, 41 -- Received: from ndb-mr3.cc.emory.edu (ndb-mr3.cc.emory.edu [170.140.53.253]) 11, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1A3fUMU029504 11, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 21:41:30 -0600 11, 41 -- Received: from e14edge2w.Emory.Edu (e14edge2w.eu.emory.edu [170.140.108.22] (may be forged)) 11, 41 -- by ndb-mr3.cc.emory.edu (8.13.8/8.13.8) with ESMTP id t1A3fSPD026892 11, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2015 22:41:29 -0500 11, 41 -- Received: from E14CH1N.Enterprise.emory.net (10.240.8.113) by 11, 41 -- e14edge2w.Emory.Edu (170.140.108.22) with Microsoft SMTP Server (TLS) id 11, 41 -- 14.3.224.2; Mon, 9 Feb 2015 22:41:28 -0500 11, 41 -- Received: from E14MBX11W.Enterprise.emory.net ([fe80::2cf2:45a4:a1cc:51f7]) by 11, 41 -- e14ch1n.Enterprise.emory.net ([::1]) with mapi id 14.03.0174.001; Mon, 9 Feb 11, 41 -- 2015 22:41:28 -0500 11, 41 -- From: "Yi, Hong" {hyi-at-emory.edu} 11, 41 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 41 -- Subject: Re: [Microscopy] anti-catalase 1Ab for TEM 11, 41 -- Thread-Topic: [Microscopy] anti-catalase 1Ab for TEM 11, 41 -- Thread-Index: AQHQRL55aOiDc8+gAEiLbAq4L7mFFJzpPW2A 11, 41 -- Date: Tue, 10 Feb 2015 03:41:28 +0000 11, 41 -- Message-ID: {D0FEE5AD.155F2%hyi-at-emory.edu} 11, 41 -- References: {201502092316.t19NGdf1021960-at-ns.microscopy.com} 11, 41 -- In-Reply-To: {201502092316.t19NGdf1021960-at-ns.microscopy.com} 11, 41 -- Accept-Language: en-US 11, 41 -- Content-Language: en-US 11, 41 -- X-MS-Has-Attach: 11, 41 -- X-MS-TNEF-Correlator: 11, 41 -- user-agent: Microsoft-MacOutlook/14.4.7.141117 11, 41 -- x-originating-ip: [71.236.2.128] 11, 41 -- Content-Type: text/plain; charset="us-ascii" 11, 41 -- Content-ID: {ED19DE58AAC0244BA26B6C69701DE399-at-Enterprise.emory.net} 11, 41 -- MIME-Version: 1.0 11, 41 -- X-Emory-MailScanner-Information: Please contact the ISP for more information 11, 41 -- X-Emory-MailScanner-ID: t1A3fSPD026892 11, 41 -- X-Emory-MailScanner: Found to be clean 11, 41 -- X-Emory-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 11, 41 -- score=-0.01, required 8, autolearn=disabled, 11, 41 -- T_RP_MATCHES_RCVD -0.01) 11, 41 -- X-Emory-MailScanner-From: hyi-at-emory.edu 11, 41 -- X-Spam-Status: No 11, 41 -- Content-Transfer-Encoding: 8bit 11, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1A3fUMU029504 ==============================End of - Headers==============================
The Southeastern Microscopy Society would like to invite you to join us for the Society's 51st Annual meeting to be held May 20-22, 2015. The meeting will be held at the Courtyard Marriott hotel in downtown Decatur, GA located at 130 Clairemont Avenue. Further, I would encourage you and your students to consider submitting an abstract for a poster or platform presentation at the meeting.
All information regarding the meeting can be found on the SEMS website. Please visit http://southeasternmicroscopy.org/2015-meeting/ for registration and the Call for Papers. All abstracts should be submitted by April 17. Students should be encouraged to present their research through the Ruska competition. Ruska participants receive meeting registration, housing at the meeting hotel (applicants must be willing to share a room with other students), a banquet ticket, and a certificate of participation. The first place Ruska Award winner will receive the Ruska Award plaque and $300.00 at the SEMS banquet. This is great opportunity for students!
The Invited speakers will be Dale Newbury, with NIST; Lucille Giannuzzi, with L.A. Giannuzzi & Associates; and Peter Kner, with the University of Georgia. In addition, Robert Simmons from Georgia State University has agreed to talk about Microscopy and Art for the banquet.
The meeting will start Wednesday, May 20, with workshops and technical talks. Wednesday evening we will have the Mixer and poster session starting at 6 PM. Thursday's schedule contains invited and submitted presentations and opportunities to visit our vendors. The Banquet will be Thursday evening. Plan to attend the Business Breakfast on Friday morning and finish off attending some final presentations, with the meeting ending at noon.
Hotel rooms will cost $129 + tax, and reservations can be made at http://cwp.marriott.com/atldc/sems or by calling the hotel at 404-371-0204 at asking for the SEMS special rate. Reservation must be made by April 28 to receive this rate.
Russ Goddard SEMS President
Amanda Lawrence Outreach Coordinator/Research Associate Institute for Imaging and Analytical Technologies Mississippi State University
==============================Original Headers============================== 11, 31 -- From ALawrence-at-i2at.msstate.edu Tue Feb 10 13:24:45 2015 11, 31 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1AJOjo9000614 11, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2015 13:24:45 -0600 11, 31 -- Received: from mail05.ad.msstate.edu (mail05.ad.msstate.edu [130.18.230.64]) 11, 31 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id t1AJOixd008628 11, 31 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 11, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2015 13:24:45 -0600 11, 31 -- X-Sender: {} 11, 31 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by 11, 31 -- mail05.ad.msstate.edu (2002:8212:e640::8212:e640) with Microsoft SMTP Server 11, 31 -- (TLS) id 15.0.913.22; Tue, 10 Feb 2015 13:24:41 -0600 11, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 11, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 11, 31 -- 15.00.0913.011; Tue, 10 Feb 2015 13:24:41 -0600 11, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 11, 31 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 11, 31 -- Subject: Southeastern Microscopy Society (SEMS) Annual Meeting 11, 31 -- Thread-Topic: Southeastern Microscopy Society (SEMS) Annual Meeting 11, 31 -- Thread-Index: AdBFZlZFVh92mi/UQeC30qvCK0LQLA== 11, 31 -- Date: Tue, 10 Feb 2015 19:24:40 +0000 11, 31 -- Message-ID: {ba551c2f7b7c454391c2540a94b9192c-at-mail02.ad.msstate.edu} 11, 31 -- Accept-Language: en-US 11, 31 -- Content-Language: en-US 11, 31 -- X-MS-Has-Attach: 11, 31 -- X-MS-TNEF-Correlator: 11, 31 -- x-originating-ip: [130.18.230.93] 11, 31 -- Content-Type: text/plain; charset="us-ascii" 11, 31 -- MIME-Version: 1.0 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1AJOjo9000614 ==============================End of - Headers==============================
We are submitting a NSF proposal for new equipment and came across a requirement for a data management plan. At this moment my facility users are responsible for their own data. I'm interested to find out what others are using for their data management plans. We are a "Google" university (Mail/Calendar etc.) - would storing data on their Drive folder be adequate?
Thanks in advance!
Owen
Owen Mills Michigan Tech University Houghton, MI
==============================Original Headers============================== 5, 34 -- From opmills-at-mtu.edu Tue Feb 10 13:53:35 2015 5, 34 -- Received: from mail-ie0-f178.google.com (mail-ie0-f178.google.com [209.85.223.178]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1AJrZ1l024383 5, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2015 13:53:35 -0600 5, 34 -- Received: by iebtr6 with SMTP id tr6so27420226ieb.4 5, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2015 11:53:35 -0800 (PST) 5, 34 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 34 -- d=1e100.net; s=20130820; 5, 34 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 5, 34 -- :subject:content-type:content-transfer-encoding; 5, 34 -- bh=HnimjtjZhVRMLF79perpaxDSN40i0rFTOVsqA/8JmdI=; 5, 34 -- b=e3PqhNDPGdiuaPLx9dw7QBc4LucCNhWFXgwpuZL7bMCDqMNfd7GP5q2PUBwfDNmtyL 5, 34 -- rsA+KMADaRvKXGXpLoZPpF6pFrDZ2lLdEmCakGD9MNH3aZ7gu7h5Aahx0dZRLpQbNVq4 5, 34 -- aC0Lsqq9RIvfPGMRayHRmQrjEcgRTjdxDyVshU/bPpVfiThwZ/vdaGSF6ZKiW3s4BI6A 5, 34 -- JXPDts7t1j1iVTlEG2kj31rFsrFkyR0/R8SNl0w8pZ1GLPueHDWOJCi84C9Evu2hffML 5, 34 -- 9/aQbpUgouapf79nw8bJam+BMzJyUR1VqnPu7H3q1+qWKcHHO2VIp9vVBIS/s4UAcWm3 5, 34 -- Lx8g== 5, 34 -- X-Gm-Message-State: ALoCoQlDX7DWHDm8NhRAPdbedu+XdtTCfZKc+cNYnt699hNb0O5h73RirBXWFEqhVylKSw2/JcRw 5, 34 -- X-Received: by 10.43.140.68 with SMTP id iz4mr27964599icc.77.1423598013948; 5, 34 -- Tue, 10 Feb 2015 11:53:33 -0800 (PST) 5, 34 -- Received: from e12-0626-fac.coe.ad.mtu.edu (e12-0626-fac.coe.ad.mtu.edu. [141.219.30.140]) 5, 34 -- by mx.google.com with ESMTPSA id m77sm9196236iom.38.2015.02.10.11.53.32 5, 34 -- for {Microscopy-at-microscopy.com} 5, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 5, 34 -- Tue, 10 Feb 2015 11:53:33 -0800 (PST) 5, 34 -- Message-ID: {54DA627E.4090400-at-mtu.edu} 5, 34 -- Date: Tue, 10 Feb 2015 14:56:46 -0500 5, 34 -- From: Owen Mills {opmills-at-mtu.edu} 5, 34 -- User-Agent: Postbox 3.0.11 (Macintosh/20140602) 5, 34 -- MIME-Version: 1.0 5, 34 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 34 -- Subject: NSF data management plans 5, 34 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 34 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: MICROSCOPY SOCIETY OF NORTHEASTERN OHIO
Title-Subject: [Filtered] MSNO 2015 Meeting
Message: I'd like to invite you to join MSNO Winter Meeting on Wednesday, March 4th, 2015, 3:00 –8:30 p.m. at Cleveland Museum of Natural History.
Lillian A. Kuri from Cleveland Foundation will give a talk on "ClevelandÂ’s Greater University Circle Initiative" and John Hemsath, a Director of Theater Operations will give a talk on "The History of Playhouse Square".
This meeting will give us two successful demonstrations on how collaboration could make a wonderful impact and we could also make such a significant impact to our society too.
Pre-registration and more detail could be found at http://www.msneo.org/2015-winter-meeting.html
We hope to see you at the meeting.
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Email: kpseverin-at-alaska.edu Name: Ken Severin
Organization: University of Alaska Fairbanks
Title-Subject: [Filtered] HV cable for Ladd sputter coater
Message: The HV cable on my old Ladd/Polaron (also sold as ISI and a few other brands) went up in smoke. If someone has a spare or source (or a simple sputter coater that is no longer in use) please contact me off line and we'll work out a deal.
Thanks much!
Ken Severin University of Alaska Fairbanks kpseverin-at-alaska.edu
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Email: bob.price-at-uscmed.sc.edu Name: Bob Price
Organization: University of South Carolina
Title-Subject: [Filtered] Workshop on Basic Confocal Microscopy
Message: During the week of June 15-19 the University of South Carolina Instrumentation Resource Facility will again host the "Basic Confocal Microscopy Workshop." Workshop material is directed towards beginning and intermediate users of confocal microscopes and involves a series of lectures (specimen preparation, labeling strategies, proper set-up of instrument operating parameters, proper handling of 2D and 3D confocal images in programs such as Adobe Photoshop and AMIRA), hands on specimen preparation, and time on a number of different point scanning and spinning disk confocal microscopes.
This is a hands on workshop where participants will stain cells and tissues with multiple labels, collect images, and participate in image analysis exercises using Photoshop and AMIRA.
Faculty will include Dr. Jay Jerome of Vanderbilt University, Dr. Ralph Albrecht of the University of Wisconsin Madison, Dr. John Mackenzie of North Carolina State University, Dr. Tom Trusk of the Medical University of South Carolina, and Dr. Bob Price of the University of South Carolina. Several companies will also have equipment and applications experts on hand to assist with instrumentation and questions about confocal microscopy research protocols.
Cost for the entire week is $350 which covers meals and supplies for the workshop. Please register soon as the workshop has filled the past several years.
For further information please see: http://irf.med.sc.edu/
For questions and registration contact Anna McNeal (anna.mcneal-at-uscmed.sc.edu or Bob Price (bob.price-at-uscmed.sc.edu
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On Feb 9, 2015, at 10:30 AM, frank_karl-at-ardl.com wrote:
} Hello Everyone, } I'm looking for suggestions about a beam sensitive sample. } } I'm cutting thin sections with a cryomicrotome of HIPS, High Impact } Polystyrene, that is toughened with polybutadiene. I'm cutting at } -100 C because of the low TG of the polybutadiene and staining with } Os. } My thin sections are very beam sensitive, too sensitive to focus } well and get a good image. I'm imaging at 80KV with 8800X } magnification. If I spread out my beam to reduce beam damage I } can't see my sample to focus and any pictures I take look crappy. } } Any suggestions? } } I've been thinking about trying carbon coated grids to help act as a } heat sink as well as going to a larger TEM spot setting. I have read } that lower KV can make the damage worse because each electron } resides in the thin section longer and produced more heat. Is this } a tried and true observation? } } Thanks!!! } } Frank } } This email and any of its attachments may contain confidential } information intended only for the use of the addressee(s). If the } reader of this email is not the intended recipient or the employee } or agent responsible for delivering it to the intended recipient, } you are hereby notified that any dissemination or copying of this } email is strictly prohibited. If you have received this email in } error, please notify us by return email at info-at-ardl.com, } permanently delete the email, and destroy any printouts. If this } email contains test data and/or draft reports, you are hereby } notified that only a signed original test report will contain } official results, a copy of which resides in the project folder } located at ARDL, Inc. Thank you. Akron Rubber Development } Laboratory, Inc. } } Dear Frank, Since you have cryosectioning capability, can I assume you have cryomicroscopy capability? If so, do you have a low-dose capability? In addition to carbon-coating, you could put a few gold nanoparticles onto the specimen, use the low-dose settings to find an area a micrometer or so away from the imaging area, focus pretty quickly on the gold beads--easier with FEG or very coherent LaB6--then take the image. You could even burn a small hole at the focussing position (assuming that the specimen can survive that) without having the beam overlap the imaging position. If you do not have low-dose capability, you might try using an image shift setting to get to a focussing area-- calibrate with a cross grating--and return the shift to its original setting for imaging. All this shifting should be done with a pre- specimen shutter engaged between shifts to avoid unexpected exposure of the specimen. Good luck. Yours, Bill
==============================Original Headers============================== 6, 32 -- From wtivol-at-sbcglobal.net Wed Feb 11 20:46:34 2015 6, 32 -- Received: from nm5-vm4.access.bullet.mail.gq1.yahoo.com (nm5-vm4.access.bullet.mail.gq1.yahoo.com [216.39.63.93]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1C2kYQJ024088 6, 32 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2015 20:46:34 -0600 6, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1423709194; bh=ANalGMYfWm6XiJl1uxYSFAp+6o/TDeIS1aVl4jtfvEg=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=ijGr3i0bOs/+0l4Rt1vv4Tp0R2TI/3vQOwgZAY2kPkI/pt4taPMEsgy6A/35FvE9swbCswyOjV14SQJpIKGowMR8KeHaNT/YpfAIGXfKUcBQzswV+AXTYJ3AkRToYv6/5HGLUhQhWnssuWRzFS2FgGhwu3M/0vo+QckJyDIllt0SRV5lt5G6t+mybp8x8aEYGznWttfKoPj9QVQwylae+L+BeoN9YV2LHz2ckpNue1yrGsMwGL229qELV9JP1CAny+36SuTrm/j4abyGay+hBldoj+NjaW3JiK5zOHHIcBBqoKODQ99sMX82pseZB7LVMdT+DU+CEGUA59e7WEfbzA== 6, 32 -- Received: from [216.39.60.170] by nm5.access.bullet.mail.gq1.yahoo.com with NNFMP; 12 Feb 2015 02:46:34 -0000 6, 32 -- Received: from [67.195.23.144] by tm6.access.bullet.mail.gq1.yahoo.com with NNFMP; 12 Feb 2015 02:46:34 -0000 6, 32 -- Received: from [127.0.0.1] by smtp116.sbc.mail.gq1.yahoo.com with NNFMP; 12 Feb 2015 02:46:34 -0000 6, 32 -- X-Yahoo-Newman-Id: 79688.63392.bm-at-smtp116.sbc.mail.gq1.yahoo.com 6, 32 -- X-Yahoo-Newman-Property: ymail-3 6, 32 -- X-YMail-OSG: 5hWdtEUVM1n8w3XKR2m3i8j.yR.n2xvTFFJTMLjogU.HxIC 6, 32 -- A4fwnhGP3P2fQ7qodSqVCUmP.bVnI9iqWkroyeaORc9anoa00KHetgSq2ISp 6, 32 -- TuQcAQvJGQ6ijAQGQJopvYwkcDn88YIhgfdd_Ptq5ldCB7Oq2IolVstIrGnc 6, 32 -- AHaGYhwfeHX8u55SF8A2.751_vT6Lw2SKQtz_h5sjd40wEZqxJDPdppEXObj 6, 32 -- j826fGUmBcZ65nRqlGzJ03zyVqrn4trGd9ZKLeT0n9trYJX5pEvqCgFUuKJS 6, 32 -- vjYoxIIDp2ZS07DKeS.0aQ93q0mt1y6iv7NmDG6IOZlEic13c93.eC.VnMRB 6, 32 -- RY8QMSLJ1PHyyX5svejJprItCHMmKx5K78.MfPWyojDFx3ODXA.C3SfN2LD. 6, 32 -- AYu3u68hz.L4273he2O8NhEhnc6cJPKc8W1mqRcC85RCYBkx7lm0KSj.g668 6, 32 -- iJlNTdDq9WvEgJ8gg.TuXbEkwb6fQWslQ9HdxkuyrKPhbIzpHW3VnxIiJcW. 6, 32 -- csnZUnBxAXXvdDoU.SvTssYI3hc7DRS4.v1cr 6, 32 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 32 -- Message-Id: {C445CC56-20C1-44E8-A4F6-6E41F3F766C0-at-sbcglobal.net} 6, 32 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 32 -- To: Frank Karl {frank_karl-at-ardl.com} , microscopy-at-microscopy.com 6, 32 -- In-Reply-To: {201502091830.t19IULUS002451-at-ns.microscopy.com} 6, 32 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 32 -- Content-Transfer-Encoding: 7bit 6, 32 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 32 -- Subject: Re: [Microscopy] TEM and HIPS 6, 32 -- Date: Wed, 11 Feb 2015 18:46:30 -0800 6, 32 -- References: {201502091830.t19IULUS002451-at-ns.microscopy.com} 6, 32 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
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Email: rms-at-angstrom.us Name: Bob Sommerville
Organization: Angstrom Scientific Inc.
Title-Subject: [Filtered] UCLA Workshop on Imaging Kinetics at the Atomic Level
Message: Wednesday, February 18th at the UCLA California NanoSystems Institute 570 Westwood Plaza, Building 114 Los Angeles, CA 90095 9:30am: Invited Talks (Executive Conference Room) 1:30pm: Live Demonstrations (Room B145) Please RSVP to: rms-at-angstrom.us ** Talks will be followed by lunch and then a live demonstration of the capabilities of the DENSsolutions heating holder on the EICNÂ’s FEI Titan Krios. We encourage participants to bring samples. Invited talk by Dr. Paul Voyles Professor, Materials Science and Engineering, University of Wisconsin-Madison Atomic Rearrangements in Glass-Forming Metal Alloys Melted Inside the STEM Atomic rearrangements in the liquid state are fundamental to transformations of materials including crystallization and the glass transition, but they occupy a difficult to access regime of time- and length-scale. For glass-forming liquids, the relaxation time, which is the characteristic time for atoms to switch neighbors, ranges from microseconds or less in the high-temperature equilibrium liquid to hundreds of seconds in the deeply supercooled liquid near the glass transition temperature, Tg. The length scale is likely to be on the nanometer to sub-nanometer scale, but is largely unknown. We have used time-resolved coherent electron nanodiffraction in the STEM to measure for Pd40Ni40P20 alloy in the supercooled liquid from Tg to Tg + 40 K with 2 nm spatial resolution. This new experimental technique, called electron correlation microscopy, is the electron equivalent of x-ray photon correlation spectroscopy, but at higher spatial resolution. It is made possible by recent advances in electron microscope instrumentation, including highly coherent electron sources, high stability heating holders, and fast electron detectors. Additional presentations: MaterialÂ’s kinetics at the atomic level Eric Kievit, Director of Sales, DENSsolutions Revealing sintering behavior of SiC nanoparticles by in-situ heating HRTEM Dr. Lianyi Chen, Department of Mechanical and Aerospace Engineering, UCLA DENSsolutions Provides Sample Management Solutions for in-Situ Electron Microscopy Angstrom Scientific Inc. is a US Distributor providing characterization solutions to the nanotech marketplace. Angstrom supports DENS throughout the US as well as other principals such as Kleindiek and Hitachi for Tabletop TM 3030 SEM's and for key products and accessories related to TEM, SEM and Dual Beam markets
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Email: ech-at-uvic.ca Name: Elaine Humphrey
Organization: University of Victoria
Title-Subject: [Filtered] M&M 2015 Family Affair ProjectMICRO and Foldscopes
Message: Dear All Last year in Hartford at the M&M the Solve the Mystery was based on the story: Secret Agent, James B Atom, wrote a message for Headquarters. It was written on a FIB and could only be read on an sem (too small for a light microscope. It was cut into four pieces and your task should you choose to accept it, is to take your piece of the message to the Q's lab (Exhibit Hall where we have some vendors to work with you) to read it. Once deciphered you should take it to Headquarters (the Outreach Booth) where it will be put together.
The message read "Never trust an Atom, they make up everything"
Everyone who attended seemed to particularly enjoy this bit, so I was thinking of doing it again but with a different message. Any ideas?
Also I put our name down for some Foldscopes (origami microscopes) which have just arrived and I intent to bring them to the Family Affair at M&M. I hope to have one at the Outreach booth too. http://www.foldscope.com/
The Project Micro workshop since we will be in Portland, will be the ever popular "The Private Eye workshop" http://the-private-eye.com/index.html This is one of the best outreach workshops to help Elementary School Teachers get sucked into Science even though they may be intimidated by Science. They use a 5x loupe.
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I¹m sure this question has been asked many times before, but I wanted to see if people might share their systems for keeping track of TEM samples. In grad school it wasn¹t too bad, since I was focused mainly on my own samples, but now I¹ve been deluged with samples from many different projects.
I¹m considering using Evernote in combination with a naming and tracking scheme, but I¹m open to other options as well.
Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
Hi Steven, X-from my experience, having good tracking, a naming method and physical storage for samples is key. I would suggest going beyond a simple text file, as this becomes unwieldy quickly. A database can be as simple as a spreadsheet, with columns for all the parameters of interest and links to image storage locations. A spreadsheet can be filtered and searched and doesn't require any programming - unless you need to do a real database for PNNL tracking or billing. Set viewing and editing permissions so that only those you trust to enter data properly can make changes. A physical organizer for grid boxes, with names that relate to your database for getting at them later if desired, is really helpful. Good luck! Regards, Larry Scipioni ZS Genetics
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: Thursday, February 12, 2015 6:57 PM To: LES-at-ZSGENETICS.COM
Hello everyone,
I¹m sure this question has been asked many times before, but I wanted to see if people might share their systems for keeping track of TEM samples. In grad school it wasn¹t too bad, since I was focused mainly on my own samples, but now I¹ve been deluged with samples from many different projects.
I¹m considering using Evernote in combination with a naming and tracking scheme, but I¹m open to other options as well.
Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
We give each project a unique project number, which is incorporated into sample labels, processing records and image records, as well as a project database (currently Filemaker pro) so every step can be traced back to the project. Ours is prefixed by an identifying code, followed by the financial year, followed by the project number - ie, EMLP 14-15.037
The database includes client name, department (or company), order number, brief description of work, financial details etc etc. Each project has a processing protocol and job summary that is filed in a folder headed with the corresponding project number.
This way, as long as you've got the project number, you can refer back to any sample.
We also give each image a unique image number for the same reason.
All the best,
Natalie
Miss Natalie Allcock CBS Electron Microscopy Facility University of Leicester
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: 12 February 2015 23:58 To: Allcock, Natalie S.
Hello everyone,
I¹m sure this question has been asked many times before, but I wanted to see if people might share their systems for keeping track of TEM samples. In grad school it wasn¹t too bad, since I was focused mainly on my own samples, but now I¹ve been deluged with samples from many different projects.
I¹m considering using Evernote in combination with a naming and tracking scheme, but I¹m open to other options as well.
Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
We've got an old Model 8000 IMIX PC EDS system lying around made by Princeton Gamma Tech and we were wondering if anyone might need it for spare parts, or whatever. Let us know.
ERiC Jay Miller Microscopy & Imaging Specialist Electron Probe Instrumentation Center
Northwestern University Mail: 2036 Cook Hall Office: 1152 Cook Hall 2220 Campus Drive Evanston, IL 60208-3108
ph: (847) 467-0789
http://www.nuance.northwestern.edu
==============================Original Headers============================== 9, 56 -- From eric-miller-at-northwestern.edu Fri Feb 13 17:02:19 2015 9, 56 -- Received: from evcspsym1.ads.northwestern.edu (evcspsym1.ads.northwestern.edu [129.105.238.5]) 9, 56 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1DN2Joi027678 9, 56 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Feb 2015 17:02:19 -0600 9, 56 -- X-AuditID: 8169ee05-f797b6d0000014e3-fd-54de827b2d6e 9, 56 -- Received: from evcspprf3.ads.northwestern.edu (evcspprf3.ads.northwestern.edu [165.124.82.242]) 9, 56 -- by evcspsym1.ads.northwestern.edu (Symantec Messaging Gateway) with SMTP id C5.6D.05347.B728ED45; Fri, 13 Feb 2015 17:02:19 -0600 (CST) 9, 56 -- Received: from pps.filterd (evcspprf3.ads.northwestern.edu [127.0.0.1]) 9, 56 -- by evcspprf3.ads.northwestern.edu (8.14.7/8.14.7) with SMTP id t1DM1hnd025981 9, 56 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Feb 2015 15:02:14 -0800 9, 56 -- Received: from evcspcas3.ads.northwestern.edu (evcspcas3.ads.northwestern.edu [129.105.68.135]) 9, 56 -- by evcspprf3.ads.northwestern.edu with ESMTP id 1sh0xq0vvq-1 9, 56 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Feb 2015 15:02:14 -0800 9, 56 -- Received: from EVCSPMBX3.ads.northwestern.edu ([169.254.3.67]) by 9, 56 -- EVCSPCAS3.ads.northwestern.edu ([129.105.68.135]) with mapi id 9, 56 -- 14.03.0158.001; Fri, 13 Feb 2015 17:02:18 -0600 9, 56 -- From: Eric Jay Miller {eric-miller-at-northwestern.edu} 9, 56 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 56 -- Subject: IMIX-PC EDS by PGT 9, 56 -- Thread-Topic: IMIX-PC EDS by PGT 9, 56 -- Thread-Index: AdBH4MQ2XraouV8ATxKz66/43CQ+yg== 9, 56 -- Date: Fri, 13 Feb 2015 23:02:17 +0000 9, 56 -- Message-ID: {1A377C4705E2B14C8D55336F80E66047018EA533-at-evcspmbx3.ads.northwestern.edu} 9, 56 -- Accept-Language: en-US 9, 56 -- Content-Language: en-US 9, 56 -- X-MS-Has-Attach: 9, 56 -- X-MS-TNEF-Correlator: 9, 56 -- x-originating-ip: [165.124.43.167] 9, 56 -- Content-Type: text/plain; charset="us-ascii" 9, 56 -- MIME-Version: 1.0 9, 56 -- X-Proofpoint-Virus-Version: vendor=nai engine=5400 definitions=5800 signatures=585085 9, 56 -- X-Proofpoint-Spam-Details: rule=audit_notspam policy=audit score=0 spamscore=0 suspectscore=0 9, 56 -- phishscore=0 adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx 9, 56 -- scancount=1 engine=7.0.1-1402240000 definitions=main-1409170064 9, 56 -- X-Brightmail-Tracker: H4sIAAAAAAAAA02TbUxTVxjH89y+eEEuXgqUx8rI1sxpfEE0W7LhavywD7DNZM4Sk22JVHql 9, 56 -- HbWw3oLCNgSCQ5DNEjq0ZdrCkBXXrBlhxgBGVrepEOaI8XXYieDGS3GbEIWtuJ3bF+iXm+f+ 9, 56 -- /uf5n/85Tw4tknXFKGi90cyZjBqDUhorPv3R2482fljlU2c0NyS9HLhYsWw7ZP19dIp6C96J 9, 56 -- fVXLGfQlnGnTttxYXe/3N6miAdFBl8MuqYBpqg5oGtkX0XJcVgcxpJTjLz6PtA5iaRk7ATg/ 9, 56 -- 66BCP3cAJy8dDisjgA1XXeLQTxegbfCWROiXEquFoWapYJvEbsUfO1UCTmRTcXbuuiSEn8OB 9, 56 -- CbGAk9h0tJ61gFCL2dVYc+3sMqFm2Bw83GQJOgJJ9KTfTQm1iE3BO2MOKpQ0AVube0WR1E+7 9, 56 -- R6Shwyhx+EZuaPkGdPY8kobq9djeMiUK2SfgFduYONSag+P+r4InQXYKsH3eCRZIsUdtZ4/y 9, 56 -- skd52aO8nCA+A2lcSR5fxJfu35yu0fLpxkKTWXeA44U5pXPa4k4go6rUP5Scg2ONG73A0qCM 9, 56 -- Yyxv3lXLJJoS0uaFN2hKmcx4PvapZfF7C7WlOg2v22MqNnC8MonxVRDMLOK9xYYCpYJRVxKa 9, 56 -- uEiN3AHewJnJnl5AWkTatu0U2rSa0jLOVBgy88IqWqxMYeZjhtQyNl9j5go4rogzRVQ1TSuR 9, 56 -- uSfsl2Di8rmD+/QGc0QmfU90RGGjlWCYZ5j3M4kgjxai81B0jBey6DgSyiCkZvgizX5enx/2 9, 56 -- TWSKhR3jIjTouZIpEaAsApf8+iGPdtZcsFEysbHQyClSGFowZYWVumLjYl6FnEm7R+54RZQg 9, 56 -- WCtSma2jhCdH8SX3yLOahACQOSUy/wWzkVe3FFjGvFJO4PIwDOZFpiZ4bWG2ZLili/iwQxT6 9, 56 -- na0MftZWG49z/vMrcGzBn4wnZqvk6GuoewE/P+Veg23tThW6rsxlY8/lgBpdE905WF/bthvn 9, 56 -- hy7sRvvMxX342+1PCvDU0y8MONPxoBzdoz2VgH3DjipA67HAEcC/OmtrAS/b/iFfz8JoPeD9 9, 56 -- 6REr4MzjqzbA4aOftgDeGj7dSnjH8T7A8c7GnwB/6JwfALR0tw4C+i+ND8IkGRhFBtZw4ldh 9, 56 -- YGaNOXpgboHGRWh4YGcEKIvApRtQVEDmyco8+jVr4Jve7363vrfzy1RV+Y7tetX97kBNRj99 9, 56 -- 6M9nDdWqdzPiV16/kZ2dNfet+4M1ezyvp/XrWyTXmtZVa9cONw3Yfi5bmzP9R3WjRT3uul3v 9, 56 -- Gaf7fY8fDm5K7ct1fJ2yYbnD0td8chV2bPn3+ZurXzqfuV5+6FxZ+667ux4ccXUpxbxOs3md 9, 56 -- yMRr/gcYypyJRwUAAA== 9, 56 -- Content-Transfer-Encoding: 8bit 9, 56 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1DN2Joi027678 ==============================End of - Headers==============================
I want to thanks all the advice I received about cutting cryothin sections of HIPS. The carbon coated grids worked great as did increasing the KV to 100. I stained my thin sections on the grids with OsO4 vapor for two hours and that made the phase really "POP!"
Thanks again. Now I'm hip about HIPS.
Frank
PS, I just really want more out of office remarks, it's like cowbell, how can you have too much!!!
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 9, 28 -- From frank_karl-at-ardl.com Wed Feb 18 07:12:05 2015 9, 28 -- Received: from cal1-mh779.smtproutes.com (cal1-mh779.smtproutes.com [208.70.91.145]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1IDC4kR016686 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 18 Feb 2015 07:12:04 -0600 9, 28 -- X-Katharion-ID: 1424265096.26986.cal1-mh779 9, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by 9, 28 -- cal1-mh779.smtproutes.com [(192.69.16.145)] with ESMTP via TCP 9, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 18 Feb 2015 13:11:36 +0000 9, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 9, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Wed, 18 9, 28 -- Feb 2015 08:11:36 -0500 9, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 9, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 9, 28 -- {microscopy-at-microscopy.com} 9, 28 -- Date: Wed, 18 Feb 2015 08:11:35 -0500 9, 28 -- Subject: Thanks !!! 9, 28 -- Thread-Topic: Thanks !!! 9, 28 -- Thread-Index: AdBLfGutJoPPrJaKSYq7WxmAjNbh7Q== 9, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C88CAFE59-at-exchange2k7.ad.ardl.com} 9, 28 -- Accept-Language: en-US 9, 28 -- Content-Language: en-US 9, 28 -- X-MS-Has-Attach: 9, 28 -- X-MS-TNEF-Correlator: 9, 28 -- acceptlanguage: en-US 9, 28 -- Content-Type: text/plain; charset="us-ascii" 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1IDC4kR016686 ==============================End of - Headers==============================
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Email: gary-at-gaugler.com Name: Dr. Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] LEO 1455VP LaB6
Message: Has anyone with a LEO/Zeiss 1455VP LaB6 SEM compared the Wehnelt aperture sizes? They are 1mm and 500um. Zeiss seems to have settled on the 500um Wehnelt aperture size for LaB6 and W emitters.
Is there any feedback from users about which aperture size produces the best results?
TIA, gary g.
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Email: connellyps-at-mail.nih.gov Name: Pat Connelly
Organization: NIH/National Heart, Lung and Blood Institute
Title-Subject: [Filtered] Position open at NIH
Message: There is an opening in the National Heart, Lung and Blood Institute in Bethesda, MD for an "Electron Microscopy Senior Scientist and/or Core Director".
The posting is listed on the MSA Site [Resources} Placement Office/Job Openings] as Job ID 22114004 with a full detailed description and application directions.
The job requirements state that applicants must have a PhD. (or equivalent) and be an experienced scientist with significant experience in many aspects of electron microscopy and a record of independent scientific productivity as evidenced by citable publications. Experience with correlative light/EM imaging, novel methods development, digital image processing and analysis, cryo-EM, and 3D EM methods are highly desirable. Excellent interpersonal skills are a requirement.
I believe that the same posting is also on the ASCB Job Board.
This message would not go through the List Server as I usually post with plain text.
Pat Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491 connellyps-at-mail.nih.gov
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
Does anyone have a schematic diagram of the electronics in the Emitech K-450 carbon coater? This is NOT the K-450X which is a later version. It appears that a part failed somewhere between the pirani gauge and the meter. Having the electrical diagram just might help with tracking it down.
Tom Kremer IPS Testing 3211 E. Capitol Drive Appleton, WI 54911 920-749-3040 ext.121 tkremer-at-ipstesting.com
==============================Original Headers============================== 4, 42 -- From tkremer-at-ipstesting.com Fri Feb 20 14:08:03 2015 4, 42 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1on0061.outbound.protection.outlook.com [157.56.110.61]) 4, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1KK80EM004662 4, 42 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2015 14:08:02 -0600 4, 42 -- Received: from DM2PR0701MB1020.namprd07.prod.outlook.com (25.160.24.150) by 4, 42 -- DM2PR0701MB1018.namprd07.prod.outlook.com (25.160.24.148) with Microsoft SMTP 4, 42 -- Server (TLS) id 15.1.93.16; Fri, 20 Feb 2015 20:07:52 +0000 4, 42 -- Received: from DM2PR0701MB1020.namprd07.prod.outlook.com ([25.160.24.150]) by 4, 42 -- DM2PR0701MB1020.namprd07.prod.outlook.com ([25.160.24.150]) with mapi id 4, 42 -- 15.01.0093.004; Fri, 20 Feb 2015 20:07:52 +0000 4, 42 -- From: Tom Kremer {tkremer-at-ipstesting.com} 4, 42 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 42 -- Subject: Emitech K-450 4, 42 -- Thread-Topic: Emitech K-450 4, 42 -- Thread-Index: AdBNR71/nTN/CqK/T+qaTvJLSTRIug== 4, 42 -- Date: Fri, 20 Feb 2015 20:07:51 +0000 4, 42 -- Message-ID: {DM2PR0701MB1020500E2FE271983249312BDB2A0-at-DM2PR0701MB1020.namprd07.prod.outlook.com} 4, 42 -- Accept-Language: en-US 4, 42 -- Content-Language: en-US 4, 42 -- X-MS-Has-Attach: 4, 42 -- X-MS-TNEF-Correlator: 4, 42 -- x-originating-ip: [96.11.14.178] 4, 42 -- authentication-results: spf=none (sender IP is ) 4, 42 -- smtp.mailfrom=tkremer-at-ipstesting.com; 4, 42 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID:;SRVR:DM2PR0701MB1018; 4, 42 -- x-microsoft-antispam-prvs: {DM2PR0701MB101823EC9F4CB263BE3DE3D5B52A0-at-DM2PR0701MB1018.namprd07.prod.outlook.com} 4, 42 -- x-exchange-antispam-report-test: UriScan:; 4, 42 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:;SRVR:DM2PR0701MB1018; 4, 42 -- x-forefront-prvs: 0493852DA9 4, 42 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(189002)(199003)(38414003)(450100001)(62966003)(76576001)(229853001)(86362001)(77156002)(102836002)(74316001)(66066001)(2351001)(2501002)(110136001)(68736005)(64706001)(107886001)(40100003)(106356001)(105586002)(99286002)(92566002)(50986999)(87936001)(33656002)(19580395003)(19580405001)(101416001)(2656002)(46102003)(122556002)(2900100001)(97736003)(54356999);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR0701MB1018;H:DM2PR0701MB1020.namprd07.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 4, 42 -- received-spf: None (protection.outlook.com: ipstesting.com does not designate 4, 42 -- permitted sender hosts) 4, 42 -- Content-Type: text/plain; charset="us-ascii" 4, 42 -- MIME-Version: 1.0 4, 42 -- X-OriginatorOrg: ipstesting.com 4, 42 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 20 Feb 2015 20:07:51.5606 4, 42 -- (UTC) 4, 42 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 4, 42 -- X-MS-Exchange-CrossTenant-id: fcd656df-08de-421e-a3c5-e640523dfad4 4, 42 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR0701MB1018 4, 42 -- Content-Transfer-Encoding: 8bit 4, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1KK80EM004662 ==============================End of - Headers==============================
At the University of Rochester Medical Center's Pathology Department in Rochester, NY we are looking to replace our retiring Kidney EM Clinical Laboratory Specialist..
Please contact Karen Bentley if you have any questions about this clinical position or you can go to the University of Rochester Medical Center's website and at the bottom of the page listed under URMC Information is the "Job Opportunities" link which will lead you to the jobs, type in # 185704.
This position requires sufficient knowledge to run the day-to-day operations of both the Transmission Electron Microscope (TEM) and Immunofluorescent (IF) laboratories, under supervision of the Medical Director of Renal EM and Autopsy who is responsible for those laboratories. Required skills, which can be acquired during the initial on the job training, include knowledge of normal and abnormal ultrastructural and immunofluorescence morphology.
Bachelor's degree in Clinical Laboratory Science and one year of experience; or an equivalent combination of education, experience or certification as required for New York State licensure. Experience in field of electron microscopy, renal pathology strongly preferred, but on the job training will be provided to the right candidate.
Karen Bentley, M.S. Director Electron Microscope Research Core Pathology & Laboratory Medicine University of Rochester Medical Center 575 Elmwood Avenue Rochester, NY 14642 585-275-1954
==============================Original Headers============================== 7, 44 -- From Karen_Bentley-at-URMC.Rochester.edu Mon Feb 23 12:37:08 2015 7, 44 -- Received: from voltage3.urmc.rochester.edu (voltage3.urmc.rochester.edu [128.151.10.58]) 7, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1NIb8Vx024893 7, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Feb 2015 12:37:08 -0600 7, 44 -- Received: from proofpoint2.urmc.rochester.edu (proofpoint2.urmc.rochester.edu [128.151.10.141]) 7, 44 -- by voltage3.urmc.rochester.edu (8.14.4/8.14.4) with ESMTP id t1NIb7t9013307 7, 44 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 7, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Feb 2015 13:37:07 -0500 7, 44 -- Received: from pps.filterd (proofpoint2.urmc.rochester.edu [127.0.0.1]) 7, 44 -- by proofpoint2.urmc.rochester.edu (8.14.5/8.14.5) with SMTP id t1NIXoMI005842 7, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Feb 2015 13:37:07 -0500 7, 44 -- Received: from urmcht1.urmc-sh.rochester.edu (urmcht1.urmc.rochester.edu [128.151.10.24]) 7, 44 -- by proofpoint2.urmc.rochester.edu with ESMTP id 1sr5bh8716-1 7, 44 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NOT) 7, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Feb 2015 13:37:07 -0500 7, 44 -- Received: from urmccas3.urmc-sh.rochester.edu (128.151.10.15) by 7, 44 -- urmcht1.urmc-sh.rochester.edu (128.151.10.24) with Microsoft SMTP Server 7, 44 -- (TLS) id 8.3.389.2; Mon, 23 Feb 2015 13:36:54 -0500 7, 44 -- Received: from URMCMS4.urmc-sh.rochester.edu 7, 44 -- ([fe80:0000:0000:0000:2429:259e:33.91.196.25]) by 7, 44 -- urmccas3.urmc-sh.rochester.edu ([172.16.126.201]) with mapi; Mon, 23 Feb 2015 7, 44 -- 13:37:07 -0500 7, 44 -- From: "Bentley, Karen" {Karen_Bentley-at-URMC.Rochester.edu} 7, 44 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 7, 44 -- Date: Mon, 23 Feb 2015 13:37:07 -0500 7, 44 -- Subject: Clinical EM job opening 7, 44 -- Thread-Topic: Clinical EM job opening 7, 44 -- Thread-Index: AdBPlxoGGq/VSZ3hR3+z9GHhe4ew1w== 7, 44 -- Message-ID: {20D976085A319340B0FFF3BED71E93E80609F06EDD-at-URMCMS4.urmc-sh.rochester.edu} 7, 44 -- Accept-Language: en-US 7, 44 -- Content-Language: en-US 7, 44 -- X-MS-Has-Attach: 7, 44 -- X-MS-TNEF-Correlator: 7, 44 -- acceptlanguage: en-US 7, 44 -- Content-Type: text/plain; charset="us-ascii" 7, 44 -- MIME-Version: 1.0 7, 44 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.13.68,1.0.33,0.0.0000 7, 44 -- definitions=2015-02-23_02:2015-02-23,2015-02-23,1970-01-01 signatures=0 7, 44 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 7, 44 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 7, 44 -- engine=7.0.1-1402240000 definitions=main-1502230170 7, 44 -- X-Proofpoint-Outbound-Spam-Details: urmc_outbound_spam_policy 7, 44 -- Content-Transfer-Encoding: 8bit 7, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1NIb8Vx024893 ==============================End of - Headers==============================
Wehnelt apertures are usually selected depending upon the application. High magnification operations are best made with a small aperture, with the filament closer to the front of the Wehnelt, where as for low magnification work, moving the filament back, and using a larger aperture, provides sufficient performance with longer filament life. Those carrying out a large amount of BSE investigations will also be better served using a larger aperture as bigger sources provide higher signals.
Most manufacturers use a smaller Wehnelt aperture than for tungsten, when LaB6 filaments are fitted, as stronger bias fields are required to control the source.
Enjoy
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 20 February 2015 07:31 To: protrain-at-emcourses.com
X-from: hu.duan-at-averydennison.com
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Email: hu.duan-at-averydennison.com Name: Hu Duan
Organization: Avery
Title-Subject: [Filtered] Need your input of diamond knife
Message: Dear microscopists:
I would like to seek you experience about diamond knife. We used to use DDK diamond knife to do cryo sectioning and polishing of soft polymers. Due to supply issue, we would like to switch to another supplier.
With consideration of comparable price and performance, what would you recommend for the replacement? Or which brand/type of diamond knife you have been satisfied with cryo sectioning of soft polymers? Thank you very much.
Hu
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Title-Subject: [Filtered] Northern California Society for Microscopy Spring Meeting
Message: The NCSM spring meeting will be on Thursday, March 19 at Gatan in Pleasanton.
Please join us for dinner and excellent speakers: Ilke Arslan, of PNNL and Brigitte Papahadjopoulos-Sternberg, of NanoAnalytical Lab.
Additional details are on our website: www.ncsmicroscopy.org
We hope to see you on March 19!
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Title-Subject: [Filtered] SEM- Quanta 200 3D Control board
Message: Hi,
We are facing a problem with our FEI Quanta 200 3D (MK1 model). The microscope is extremely slow to respond to the software commands and sometimes never responds at all and the software freezes. When the scope does respond, the pumps stop working after 2 minutes of pumping and\or it is impossible to give any command to the microscope afterward. The FEI maintenance service informed us that we would have to replace the main controller board (PCB, CB2/OPTD) and\or the vaccum control board (PCB, VCB).
Did you experience this kind of problems before? How was it solved? Also, would you know of any used PCB CCB2/OPTD or VCB (from a discarded Quanta 200 3D, MK1) for sale?
Thanks a lot for your help!
Youssef
-------------------------------------- Youssef Chebli, PhD Microscopy and Imaging facility operational manager Institut de recherche en biologie végétale (514 343 6111 #82122) Department of anthropology (514 343 6111 #26901) Université de Montréal
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Hooke College of Applied Sciences, located in Westmont, IL, is offering two courses in electron microscopy.
Scanning Electron Microscopy short course March 23-27, 2015 Transmission Electron Microscopy short course April 7-9, 2015
In addition to lectures, these courses emphasize hands-on training using state-of-the-art equipment.
For further training details and registration information, please follow the link below: http://www.hookecollege.com/
Best regards- __________________________________________________ Chris Gorman Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax)
==============================Original Headers============================== 9, 41 -- From CGorman-at-hookecollege.com Wed Feb 25 14:03:50 2015 9, 41 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 9, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1PK3ofv021153 9, 41 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2015 14:03:50 -0600 9, 41 -- X-ASG-Debug-ID: 1424894629-07bbd413f2a04c0001-4CH8be 9, 41 -- Received: from TMGEX1.tmg.mccrone.com ([192.168.101.73]) by spam.mccrone.com with ESMTP id 7r9TvYXYvytMTDMf for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2015 14:03:49 -0600 (CST) 9, 41 -- X-Barracuda-Envelope-From: CGorman-at-hookecollege.com 9, 41 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by 9, 41 -- TMGEX1.tmg.mccrone.com (192.168.101.73) with Microsoft SMTP Server (TLS) id 9, 41 -- 15.0.913.22; Wed, 25 Feb 2015 14:03:48 -0600 9, 41 -- Received: from TMGEX1.tmg.mccrone.com ([::1]) by TMGEX1.tmg.mccrone.com 9, 41 -- ([fe80::5c:a323:e462:160%14]) with mapi id 15.00.0913.011; Wed, 25 Feb 2015 9, 41 -- 14:03:48 -0600 9, 41 -- From: Christine Gorman {CGorman-at-hookecollege.com} 9, 41 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 41 -- Subject: Electron Microscopy Short Courses 9, 41 -- Thread-Topic: Electron Microscopy Short Courses 9, 41 -- X-ASG-Orig-Subj: Electron Microscopy Short Courses 9, 41 -- Thread-Index: AdBRNiqxBg7FH/TASjiw5ts7vhaydA== 9, 41 -- Date: Wed, 25 Feb 2015 20:03:48 +0000 9, 41 -- Message-ID: {5d0182fcaa204b06bf6e758b2d531a2d-at-TMGEX1.tmg.mccrone.com} 9, 41 -- Accept-Language: en-US 9, 41 -- Content-Language: en-US 9, 41 -- X-MS-Has-Attach: 9, 41 -- X-MS-TNEF-Correlator: 9, 41 -- x-originating-ip: [192.168.101.154] 9, 41 -- Content-Type: text/plain; charset="us-ascii" 9, 41 -- MIME-Version: 1.0 9, 41 -- X-Barracuda-Connect: UNKNOWN[192.168.101.73] 9, 41 -- X-Barracuda-Start-Time: 1424894629 9, 41 -- X-Barracuda-URL: https://spam.mccrone.com:443/cgi-mod/mark.cgi 9, 41 -- X-Virus-Scanned: by bsmtpd at mccrone.com 9, 41 -- X-Barracuda-BRTS-Status: 1 9, 41 -- X-Barracuda-Spam-Score: 0.00 9, 41 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 9, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.15841 9, 41 -- Rule breakdown below 9, 41 -- pts rule name description 9, 41 -- ---- ---------------------- -------------------------------------------------- 9, 41 -- Content-Transfer-Encoding: 8bit 9, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t1PK3ofv021153 ==============================End of - Headers==============================
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Title-Subject: [Filtered] LEO 435 VP light panel error
Message: Hi Group!! I have a Leo 435 VP SEM and recently had some imaging problems. I noticed that on the light panel on the back of the instrument, lights #7 and 9 are blinking when the beam is on, but not when it is off or on standby. I know this is an older instrument but does anyone know what these lights indicate (i.e. what circuit board or power supply has failed)?
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==============================Original Headers============================== 10, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 25 18:34:54 2015 10, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1Q0YspU014583 10, 17 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2015 18:34:54 -0600 10, 17 -- Message-ID: {54EE6A2E.6080108-at-microscopy.com} 10, 17 -- Date: Wed, 25 Feb 2015 18:34:54 -0600 10, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 10, 17 -- MIME-Version: 1.0 10, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 17 -- Subject: viaWWW:LEO 435 VP light panel error 10, 17 -- References: {201502251653.t1PGrNCL016237-at-ns.microscopy.com} 10, 17 -- In-Reply-To: {201502251653.t1PGrNCL016237-at-ns.microscopy.com} 10, 17 -- X-Forwarded-Message-Id: {201502251653.t1PGrNCL016237-at-ns.microscopy.com} 10, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear colleagues- As part of a pitch to install remote viewing / webinar capabilities in our microscopy lab, my administration as asked me to find example systems and collect information to help us optimize and balance our wants, needs, and costs. If you have an electron microscope that you can remotely view, remotely operate, send the display output to a classroom, send the display outside your institution's network for a webinar, etc. and are willing to answer some questions about components and costs would you please contact me at brachfelds-at-mail.montclair.edu. Thank you for your time and your help, Stefanie
==============================Original Headers============================== 1, 30 -- From brachfelds-at-mail.montclair.edu Thu Feb 26 11:32:04 2015 1, 30 -- Received: from smtp.montclair.edu (smtp.montclair.edu [130.68.1.64]) 1, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1QHW4Ui020108 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 11:32:04 -0600 1, 30 -- Received: from smtp.montclair.edu (pmx-smtp1.montclair.edu [127.0.0.1]) 1, 30 -- by localhost (Postfix) with SMTP id 2992B26572 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 12:32:04 -0500 (EST) 1, 30 -- Received: from convergence2.montclair.edu (smtp.montclair.edu [130.68.1.64]) 1, 30 -- by smtp.montclair.edu (Postfix) with ESMTP id E4CFA2596C 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 12:32:03 -0500 (EST) 1, 30 -- MIME-version: 1.0 1, 30 -- Content-transfer-encoding: 7BIT 1, 30 -- Content-disposition: inline 1, 30 -- Content-type: text/plain; CHARSET=US-ASCII 1, 30 -- Received: from montclair.edu ([127.0.0.1]) by convergence2.montclair.edu 1, 30 -- (Oracle Communications Messaging Server 7u4-25.01(7.0.4.25.0) 64bit (built Feb 1, 30 -- 29 2012)) with ESMTPA id {0NKE0029G3DFT100-at-convergence2.montclair.edu} for 1, 30 -- Microscopy-at-microscopy.com; Thu, 26 Feb 2015 12:32:03 -0500 (EST) 1, 30 -- Received: from [127.0.0.1] (Forwarded-For: 130.68.125.143) 1, 30 -- by convergence2.montclair.edu (mshttpd); Thu, 26 Feb 2015 12:32:03 -0500 1, 30 -- From: "Stefanie A. Brachfeld" {brachfelds-at-mail.montclair.edu} 1, 30 -- To: Microscopy-at-microscopy.com 1, 30 -- Message-id: {76b087d53a6d28d9.54ef1243-at-montclair.edu} 1, 30 -- Date: Thu, 26 Feb 2015 12:32:03 -0500 1, 30 -- X-Mailer: Oracle Communications Messenger Express 7u4-25.01(7.0.4.25.0) 64bit 1, 30 -- (built Feb 29 2012) 1, 30 -- Content-language: en 1, 30 -- Subject: Hardware and software for remote viewing of SEM and TEM displays 1, 30 -- X-Accept-Language: en 1, 30 -- Priority: normal ==============================End of - Headers==============================
Telepresence Collaboration which is fairly old. We started doing it at ANL when the Mosaic WWW browser first appeared ~ 1994.
There are also a few old PDF's of Lectures here:
http://tpm.amc.anl.gov/Lectures/
Today, there are numerous solutions that update these older protocols.
To be honest, because of network safety (i.e. hacker) issues, I no longer allow remote control, only passive observation. I would caution you that you will need to do the same. All publically accessible sites get attacked. You don't want to know how many hackers try to break into the Microscopy Listserver every DAY.
Nestor Your Friendly Neighborhood SysOp
On Feb 26, 2015, at 11:32 AM CST, brachfelds-at-mail.montclair.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues- } As part of a pitch to install remote viewing / webinar capabilities in our microscopy lab, my administration as asked me to find example systems and collect information to help us optimize and balance our wants, needs, and costs. If you have an electron microscope that you can remotely view, remotely operate, send the display output to a classroom, send the display outside your institution's network for a webinar, etc. and are willing to answer some questions about components and costs would you please contact me at brachfelds-at-mail.montclair.edu. } Thank you for your time and your help, } Stefanie } } ==============================Original Headers============================== } 1, 30 -- From brachfelds-at-mail.montclair.edu Thu Feb 26 11:32:04 2015 } 1, 30 -- Received: from smtp.montclair.edu (smtp.montclair.edu [130.68.1.64]) } 1, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1QHW4Ui020108 } 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 11:32:04 -0600 } 1, 30 -- Received: from smtp.montclair.edu (pmx-smtp1.montclair.edu [127.0.0.1]) } 1, 30 -- by localhost (Postfix) with SMTP id 2992B26572 } 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 12:32:04 -0500 (EST) } 1, 30 -- Received: from convergence2.montclair.edu (smtp.montclair.edu [130.68.1.64]) } 1, 30 -- by smtp.montclair.edu (Postfix) with ESMTP id E4CFA2596C } 1, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2015 12:32:03 -0500 (EST) } 1, 30 -- MIME-version: 1.0 } 1, 30 -- Content-transfer-encoding: 7BIT } 1, 30 -- Content-disposition: inline } 1, 30 -- Content-type: text/plain; CHARSET=US-ASCII } 1, 30 -- Received: from montclair.edu ([127.0.0.1]) by convergence2.montclair.edu } 1, 30 -- (Oracle Communications Messaging Server 7u4-25.01(7.0.4.25.0) 64bit (built Feb } 1, 30 -- 29 2012)) with ESMTPA id {0NKE0029G3DFT100-at-convergence2.montclair.edu} for } 1, 30 -- Microscopy-at-microscopy.com; Thu, 26 Feb 2015 12:32:03 -0500 (EST) } 1, 30 -- Received: from [127.0.0.1] (Forwarded-For: 130.68.125.143) } 1, 30 -- by convergence2.montclair.edu (mshttpd); Thu, 26 Feb 2015 12:32:03 -0500 } 1, 30 -- From: "Stefanie A. Brachfeld" {brachfelds-at-mail.montclair.edu} } 1, 30 -- To: Microscopy-at-microscopy.com } 1, 30 -- Message-id: {76b087d53a6d28d9.54ef1243-at-montclair.edu} } 1, 30 -- Date: Thu, 26 Feb 2015 12:32:03 -0500 } 1, 30 -- X-Mailer: Oracle Communications Messenger Express 7u4-25.01(7.0.4.25.0) 64bit } 1, 30 -- (built Feb 29 2012) } 1, 30 -- Content-language: en } 1, 30 -- Subject: Hardware and software for remote viewing of SEM and TEM displays } 1, 30 -- X-Accept-Language: en } 1, 30 -- Priority: normal } ==============================End of - Headers==============================
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center NanoScience and Technology Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
Francoise Marga asked about imaging collagen fibers by SEM. ASM has been working with collagen fibers and monomers for over 20 years, imaging them using AFM(atomic force microscopy). The example images found on our website at:
show that AFM can image individual polymer molecules and can show subtle height variations in the fibers.
I suggest that AFM be considered for the needed analysis.
Disclosure: ASM is an independent analytical service laboratory specializing in Atomic Force Microscopy and related Scanning Probe Microscopy techniques.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: 317-895-5630 Toll free: 800-374-8557 (in USA & Canada) Fax: 317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
----- Original Message ----- From: oshel1pe-at-cmich.edu To: donc-at-asmicro.com Sent: Monday, January 12, 2015 4:59 PM Subject: [a] [Microscopy] Ask-A-Microscopist: SEM service available in Brooklyn NY area for
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Realname - Francoise Marga Email - fmarga-at-modernmeadow.com ORGANIZATION - Modern Meadow EDUCATION - Graduate College LOCATION - Brooklyn, NY, USA SUBJECT_OF_QUESTION - SEM in NYC QUESTION - Hi,
Our company would like to look at our samples by SEM. We need to go up x15,000 to visualize collagen fibers. As a business, we have trouble to find a SEM accessible to a private company. Does anyone know a facility or a private service in our area (Brooklyn, NY) that could help us. Thanks for your help. Kind regards,
Francoise
==============================Original Headers============================== 6, 31 -- From oshel1pe-at-cmich.edu Mon Jan 12 15:53:10 2015 6, 31 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t0CLrAld023845 6, 31 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2015 15:53:10 -0600 6, 31 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 6, 31 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t0CLrAee031293 6, 31 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2015 16:53:10 -0500 6, 31 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 6, 31 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with
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Title-Subject: [Filtered] Preventing charging in a TEM
Message: Hello, Does anybody use a coater to coat ceramic TEM samples with a very thin layer to mitigate charging? I would also be happy to know if there are any other ways to stop charging of samples. I am looking at manually polished complex oxide samples. Thanks! --------------------------------------------- Debangshu Mukherjee Materials Research Institute The Pennsylvania State University N-303 Millennium Science Complex University Park, PA 16802-2130 Phone: (617) 501-7316 ----------------------------------------------
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==============================Original Headers============================== 15, 16 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 28 08:02:14 2015 15, 16 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t1SE2EPw010681 15, 16 -- for {microscopy-at-microscopy.com} ; Sat, 28 Feb 2015 08:02:14 -0600 15, 16 -- Message-ID: {54F1CA66.909-at-microscopy.com} 15, 16 -- Date: Sat, 28 Feb 2015 08:02:14 -0600 15, 16 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 16 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 16 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 15, 16 -- MIME-Version: 1.0 15, 16 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 16 -- Subject: viaWWW: Preventing charging in a TEM 15, 16 -- References: {201502272306.t1RN65mp030923-at-ns.microscopy.com} 15, 16 -- In-Reply-To: {201502272306.t1RN65mp030923-at-ns.microscopy.com} 15, 16 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We've had good luck putting down a few nm (usually 3nm on our system) of Carbon to mitigate charging and to stabilize the formvar films our bio friends make. If you are doing atomic resolution STEM, make sure the C is on the bottom side.
Cheers, Henk
On 2/28/2015 9:05 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from:debangshu-at-psu.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy bothdebangshu-at-psu.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email:debangshu-at-psu.edu } Name: Debangshu Mukherjee } } Organization: The Pennsylvania State University } } Title-Subject: [Filtered] Preventing charging in a TEM } } Message: Hello, } Does anybody use a coater to coat ceramic TEM samples with a very thin layer to mitigate charging? } I would also be happy to know if there are any other ways to stop charging of samples. I am looking } at manually polished complex oxide samples. } Thanks! } --------------------------------------------- } Debangshu Mukherjee } Materials Research Institute } The Pennsylvania State University } N-303 Millennium Science Complex } University Park, PA 16802-2130 } Phone: (617) 501-7316 } ---------------------------------------------- } } Login Host: 104.39.17.35 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
I assume that Debangshu is using the highest accelerating voltage that he can with his instrument, and probably small condenser apertures and spot sizes.
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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==============================Original Headers============================== 7, 22 -- From protrain-at-emcourses.com Mon Mar 2 04:51:26 2015 7, 22 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t22ApPgq017562 7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2015 04:51:26 -0600 7, 22 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 7, 22 -- id {B54f440a60000} ; Mon, 02 Mar 2015 10:51:18 +0000 7, 22 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Mon, 2 Mar 2015 10:51:17 +0000 7, 22 -- From: "Steve Chapman" {protrain-at-emcourses.com} 7, 22 -- To: {colijn.1-at-osu.edu} , {debangshu-at-psu.edu} 7, 22 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 7, 22 -- References: {201502281753.t1SHrdm1002327-at-ns.microscopy.com} 7, 22 -- In-Reply-To: {201502281753.t1SHrdm1002327-at-ns.microscopy.com} 7, 22 -- Subject: RE: [Microscopy] Re: viaWWW: Preventing charging in a TEM 7, 22 -- Date: Mon, 2 Mar 2015 10:51:21 -0000 7, 22 -- Message-ID: {00b601d054d6$d228fb20$767af160$-at-com} 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: text/plain; 7, 22 -- charset="us-ascii" 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- X-Mailer: Microsoft Office Outlook 12.0 7, 22 -- Thread-index: AdBTf3tmVMFuO1OpTA6cBWaHIm6mZgBVoOAw 7, 22 -- Content-language: en-gb ==============================End of - Headers==============================
Hello Since the 90ies I use double sided adhesive tape for mounting SEM specimens and remember how strong these early tapes were. The last years I tried many different suppliers and all (carbon or copper) tapes I get are not very sticky, resulting in charging and beam instability problems that gives a headache. Anybody knows where to get a double sided adhesive tape for mounting SEM specimens that is really sticky? Thanks
I have a Gatan PIPS II system with Digital Micrograph. Does anyone know if there is a DM script available that can auto-terminate the ion milling process when perforation occurs? Â
-Scott Walck
==============================Original Headers============================== 3, 35 -- From s.walck-at-comcast.net Wed Mar 4 03:30:30 2015 3, 35 -- Received: from resqmta-ch2-09v.sys.comcast.net (resqmta-ch2-09v.sys.comcast.net [69.252.207.41]) 3, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t249UUUe018714 3, 35 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2015 03:30:30 -0600 3, 35 -- Received: from resomta-ch2-11v.sys.comcast.net ([69.252.207.107]) 3, 35 -- by resqmta-ch2-09v.sys.comcast.net with comcast 3, 35 -- id zMWW1p0012Ka2Q501MWWhj; Wed, 04 Mar 2015 09:30:30 +0000 3, 35 -- Received: from resmail-ch2-280v.sys.comcast.net ([162.150.49.59]) 3, 35 -- by resomta-ch2-11v.sys.comcast.net with comcast 3, 35 -- id zMWW1p0031GdifW01MWWvU; Wed, 04 Mar 2015 09:30:30 +0000 3, 35 -- Date: Wed, 4 Mar 2015 09:30:29 +0000 (UTC) 3, 35 -- From: "S.Walck" {s.walck-at-comcast.net} 3, 35 -- To: MicroscopyListserver {microscopy-at-microscopy.com} 3, 35 -- Message-ID: {1078403573.3352557.1425461429944.JavaMail.zimbra-at-comcast.net} 3, 35 -- In-Reply-To: {1718498827.3352392.1425461389483.JavaMail.zimbra-at-comcast.net} 3, 35 -- Subject: Auto-Termination Script for PIPS II 3, 35 -- MIME-Version: 1.0 3, 35 -- Content-Type: text/plain; charset=utf-8 3, 35 -- X-Originating-IP: [2601:a:6300:b917:7423:6014:1f43:8e05] 3, 35 -- X-Mailer: Zimbra 8.0.7_GA_6031 (ZimbraWebClient - IE7 (Win)/8.0.7_GA_6031) 3, 35 -- Thread-Topic: Auto-Termination Script for PIPS II 3, 35 -- Thread-Index: nncZUZ7jGz9gQIO9MAzq3R4Lckoqng== 3, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 3, 35 -- s=q20140121; t=1425461430; 3, 35 -- bh=np+b6KnP/8E9XnAXXPFalShgkLCzWyEtXT7Z20sdI5A=; 3, 35 -- h=Received:Received:Date:From:To:Message-ID:Subject:MIME-Version: 3, 35 -- Content-Type; 3, 35 -- b=CeAAgBSUNa9j8DWm/LM4VGgu/o/d7xm2R37yact1fDha9itcTiv2oURkKSjUtT0rs 3, 35 -- dlecRYPa8N1e4sGRLEDGi+1BtHY0+WtjL63abcoL0y/eA1xqT5i6paGjw6nAS/Tl7I 3, 35 -- ZuGsrpYSXO+PODk8uqccPA5zRVaki49CxEhlDXXxtOSfqhWoUCSVbvez56gRBcHnHc 3, 35 -- LmXG30t5zgQWO3okkr3VsLmOJNA8jLi1/2/iFrNvxYSW8aEmRH3BlLc33K9QWHIWik 3, 35 -- qM705L9SWrUUe/LGu1ORutuRY60uN5VG8xF3XZFqr+e0tU2rjbPfsaGA//aAN9IpYc 3, 35 -- fkhPIwsq8URgA== 3, 35 -- Content-Transfer-Encoding: 8bit 3, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t249UUUe018714 ==============================End of - Headers==============================
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Email: jarmstrong-at-ciw.edu Name: JOHN T ARMSTRONG
Organization: Carnegie Institution of Washington - Geophysical Laboratory
Title-Subject: [Filtered] Endowed support staff position - Microbeam specialist - available
Message: CARNEGIE INSTITUTION OF WASHINGTON - Vacancy Announcement –Microbeam Specialist
The Microbeam Specialist will work at the Geophysical Laboratory location in Washington, D.C.
Job Description:
The primary responsibility is to maintain and operate focused ion beam - scanning electron microscope (FIB-SEM) crossbeam system and other microbeam instruments, including training and providing assistance to new and visiting users, performing routine maintenance, sample preparation, and collaborating with staff and students. As a member of the electronics/microbeam analysis center, the person is expected to work with existing microbeam members to maintain smooth operation of the facility. The Laboratory supports world-class facilities in high-pressure research; organic, stable isotope and biogeochemistry; mineral physics and petrology; and astrobiology. See https://www.gl.ciw.edu/ for a listing of its research programs and facilities.
See details at https://www.gl.ciw.edu/content/2015/2/26/microbeam-specialist
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==============================Original Headers============================== 21, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Mar 4 06:02:41 2015 21, 17 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 21, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t24C2fLV008326 21, 17 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2015 06:02:41 -0600 21, 17 -- Message-ID: {54F6F461.1060108-at-microscopy.com} 21, 17 -- Date: Wed, 04 Mar 2015 06:02:41 -0600 21, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 21, 17 -- MIME-Version: 1.0 21, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 17 -- Subject: viaWWW:Endowed support staff position - Microbeam specialist - available 21, 17 -- References: {201503032028.t23KSe2x016429-at-ns.microscopy.com} 21, 17 -- In-Reply-To: {201503032028.t23KSe2x016429-at-ns.microscopy.com} 21, 17 -- X-Forwarded-Message-Id: {201503032028.t23KSe2x016429-at-ns.microscopy.com} 21, 17 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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} realname - Paul Webster } Email - pwebster-at-usc.edu } EDUCATION - Graduate College } LOCATION - Pasadena, CA 91107, USA } SUBJECT_OF_QUESTION - Critical Point Dryer } QUESTION - Dear All, } } I have an old Balzers Union critical point dryer FL 9496 that has been working well until now. } } The problem is that the lid of the chamber has started to leak. It may just need a gasket, but I have no idea where I could get one. } } Does anyone have information on where I could find a gasket for this part. Failing that, a source for a new lid would be welcome. } } Perhaps Technotrade is still out there and has parts for these old machines. } } Regards, } } Paul } } Paul Webster, Ph.D. } }
==============================Original Headers============================== 5, 35 -- From oshel1pe-at-cmich.edu Thu Mar 5 07:14:27 2015 5, 35 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t25DERHO026661 5, 35 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 07:14:27 -0600 5, 35 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 35 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t25DEQPf014486 5, 35 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 08:14:26 -0500 5, 35 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 5, 35 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 35 -- (TLS) id 14.3.195.1; Thu, 5 Mar 2015 08:14:26 -0500 5, 35 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 5, 35 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 5, 35 -- (TLS) id 14.3.195.1; Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 35 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 5, 35 -- 14.3.195.1; Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- Message-ID: {54F856B1.1070604-at-cmich.edu} 5, 35 -- Date: Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 35 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 35 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 35 -- MIME-Version: 1.0 5, 35 -- To: micro {microscopy-at-microscopy.com} 5, 35 -- Subject: Re: Ask-A-Microscopist 5, 35 -- References: {985736566.4028.1425508312396.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 35 -- In-Reply-To: {985736566.4028.1425508312396.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 35 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 5, 35 -- Content-Transfer-Encoding: 7bit 5, 35 -- X-Originating-IP: [141.209.2.100] 5, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 35 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 35 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 35 -- X-CanItPRO-Stream: default 5, 35 -- X-Canit-Stats-ID: 02NXNeqly - b4c40e78afdd - 20150305 5, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Don't miss the doodle honoring Momofuku Ando on the Google homepage today. There are different versions but at least one features him working at a microscope! He is presumably looking at the microscopic pores in the ramen noodles that result from flash frying and allows their rapid rehydration upon adding boiling water. Many a hungry grad student slaving over a microscope owes him a debt.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
The only posibility - I guess - to get any spare part for a Balzers CPD of old vintage in Europe (I know of, last correction in my PAB done: 2012/07) would be: ===== Info distributed by PROVAC, former supplier for BALZERS instruments and spare-parts: Date: 6 Nov 2009 "As of October 15th 2009 Baltic Präparation, Niesgrau/Germany takes over the business of Consumables for the Electron Microscopy Preparation Tecnology from Provac AG, Balzers. For many years Provac AG was a reliable partner for sales of consumables for the Elctron Microscopy Preparation Technology and we would like to thank our customers for the good cooperation and the trust in our company. Mrs. Claudia Köster from BALTIC PRÄPERATION will be the new contact for any future inquiries. For further information please contact: Mrs. Claudia Köster Baltic Präparation Koppelheck 34B D-24395 Niesgrau Germany Phone: +49 4643 18 65 43 e-mail: baltic.praeparation-at-t-online.de "
as of the (BALTIC) German website 2015-03-05: Postal address: Baltic Präparation Postfach 1116 D-24393 Koppelheck Fon 04643/186543 Fax 04643/186554 e-mail: baltic.praeparation-at-t-online.de or php-form at http://www.baltic-praeparation.de/kontakt.html
I found only a Website in GERMAN . cf: www.baltic-praeparation.de which (since 2012) still is "under construction" cf also : Products 2009 (lacking any description of a CPD-FL 9496): http://www.baltic-praeparation.de/index.php?file=tl_files/pdfs/procac2.pdf
Not knowing how they perform nowadays.... but I think it would be worth a try to request for the spare part. Best wishes, good luck, and my best regards, Wolfgang
Wolfgang MUSS EM-Lab, Univ.Inst.PATHOLOGY, SALK-LKH (Gen. Hospital) and PMU SALZBURG SALZBURG, AUSTRIA
Von: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Gesendet: Donnerstag, 05. März 2015 14:23 An: Muß Wolfgang Betreff: [Microscopy] Re: Ask-A-Microscopist: Critical Point Dryer (Spare parts for: BALZERS FL 9496)
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} realname - Paul Webster } Email - pwebster-at-usc.edu } EDUCATION - Graduate College } LOCATION - Pasadena, CA 91107, USA } SUBJECT_OF_QUESTION - Critical Point Dryer } QUESTION - } } Dear All, } I have an old Balzers Union critical point dryer FL 9496 that has been working well until now. } } The problem is that the lid of the chamber has started to leak. It may just need a gasket, but I have no idea where I could get one. } } Does anyone have information on where I could find a gasket for this part. Failing that, a source for a new lid would be welcome. } } Perhaps Technotrade is still out there and has parts for these old machines. } } Regards, } } Paul } } Paul Webster, Ph.D.
==============================Original Headers============================== 5, 35 -- From oshel1pe-at-cmich.edu Thu Mar 5 07:14:27 2015 5, 35 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t25DERHO026661 5, 35 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 07:14:27 -0600 5, 35 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 35 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t25DEQPf014486 5, 35 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 08:14:26 -0500 5, 35 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 5, 35 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 35 -- (TLS) id 14.3.195.1; Thu, 5 Mar 2015 08:14:26 -0500 5, 35 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 5, 35 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 5, 35 -- (TLS) id 14.3.195.1; Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 5, 35 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 5, 35 -- 14.3.195.1; Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- Message-ID: {54F856B1.1070604-at-cmich.edu} 5, 35 -- Date: Thu, 5 Mar 2015 08:14:25 -0500 5, 35 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 35 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 35 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 35 -- MIME-Version: 1.0 5, 35 -- To: micro {microscopy-at-microscopy.com} 5, 35 -- Subject: Re: Ask-A-Microscopist 5, 35 -- References: {985736566.4028.1425508312396.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 35 -- In-Reply-To: {985736566.4028.1425508312396.JavaMail.EWHSERVER1324$-at-10.10.133.40} 5, 35 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 5, 35 -- Content-Transfer-Encoding: 7bit 5, 35 -- X-Originating-IP: [141.209.2.100] 5, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 35 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 35 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 35 -- X-CanItPRO-Stream: default 5, 35 -- X-Canit-Stats-ID: 02NXNeqly - b4c40e78afdd - 20150305 5, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 43 -- From W.Muss-at-salk.at Thu Mar 5 08:52:44 2015 15, 43 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 15, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t25EqijD029551 15, 43 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 08:52:44 -0600 15, 43 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 15, 43 -- by hermes.salk.at (Postfix) with ESMTP id 23CD8646B87 15, 43 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 15:52:43 +0100 (CET) 15, 43 -- X-Scanned-By: SALK-Content-Filter 15, 43 -- Received: from hermes.salk.at ([127.0.0.1]) 15, 43 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 15, 43 -- with ESMTP id YHf29aeiiPZs for {Microscopy-at-microscopy.com} ; 15, 43 -- Thu, 5 Mar 2015 15:52:42 +0100 (CET) 15, 43 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 15, 43 -- by hermes.salk.at (Postfix) with ESMTP id D2F9E646B9E 15, 43 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 15:52:42 +0100 (CET) 15, 43 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 15, 43 -- by N1EX198.lks.local with ESMTP; 05 Mar 2015 16:04:03 +0100 15, 43 -- Received: from N1DT132.lksdom21.lks.local ([fe80::c9bf:82a7:c92e:ef25]) by 15, 43 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 15, 43 -- 14.03.0224.002; Thu, 5 Mar 2015 15:52:42 +0100 15, 43 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 15, 43 -- To: "'pwebster-at-usc.edu'" {pwebster-at-usc.edu} , 15, 43 -- "'Microscopy-at-microscopy.com'" 15, 43 -- {Microscopy-at-microscopy.com} 15, 43 -- CC: "'oshel1pe-at-cmich.edu'" {oshel1pe-at-cmich.edu} 15, 43 -- Subject: [Microscopy] Re: Ask-A-Microscopist: Critical Point Dryer (Spare 15, 43 -- parts for: BALZERS FL 9496) 15, 43 -- Thread-Topic: [Microscopy] Re: Ask-A-Microscopist: Critical Point Dryer 15, 43 -- (Spare parts for: BALZERS FL 9496) 15, 43 -- Thread-Index: AQHQV0eQR5NIPI/HPEWFTKhMolWKMJ0N9QFw 15, 43 -- Date: Thu, 5 Mar 2015 14:52:42 +0000 15, 43 -- Message-ID: {3D22E6EE65F39E448DCE41628153767133DCA867-at-N1DT132.lksdom21.lks.local} 15, 43 -- References: {201503051323.t25DNDT7031073-at-ns.microscopy.com} 15, 43 -- In-Reply-To: {201503051323.t25DNDT7031073-at-ns.microscopy.com} 15, 43 -- Accept-Language: de-DE, en-US 15, 43 -- Content-Language: de-DE 15, 43 -- X-MS-Has-Attach: 15, 43 -- X-MS-TNEF-Correlator: 15, 43 -- x-originating-ip: [192.168.42.2] 15, 43 -- Content-Type: text/plain; charset="iso-8859-1" 15, 43 -- MIME-Version: 1.0 15, 43 -- Content-Transfer-Encoding: 8bit 15, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t25EqijD029551 ==============================End of - Headers==============================
I survived for almost half a decade on raman noodles and learned many ways to make them including stir frying with Napa cabbage and veggies to at least pretend that it was a different dinner than the last 4000 raman noodle dinners I had. The generic ones were 8 for one buck and between that and bulk pinto beans, we managed to survive on our meager food budget which was inadequate to pay for all the beer we drank and the food too. :)
Sincerely,
James Neal-Kababick Director Flora Research Laboratories, LLC An FDA and DEA Registered & Inspected Laboratory Fellow AOAC International Vice Chair USP {2251} ADSDDA Expert Panel Adjunct Faculty Bastyr University Botanical Medicine Department 1-541-472-0980 phone jimk-at-floraresearch.com www.floraresearch.com
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-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Thursday, March 05, 2015 6:58 AM To: James Neal-Kababick
Don't miss the doodle honoring Momofuku Ando on the Google homepage today. There are different versions but at least one features him working at a microscope! He is presumably looking at the microscopic pores in the ramen noodles that result from flash frying and allows their rapid rehydration upon adding boiling water. Many a hungry grad student slaving over a microscope owes him a debt.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
A Postdoctoral position in immediately available at Northwestern University in the group of L. D. Marks (www.numis.northwestern.edu) to work in the area of tribology at the nanoscale with spillover into issues related to hip implants such as tribocorrosion and wear. While most of the work will be materials science based, aspects of it will involve collaborations with orthopedic and dental researchers and doctors. Strong experimental skills in transmission electron microscopy are essential, and experience with using Nanofactory holders (both STM and AFM) would be significant. While a background in metallurgy could be useful, it is not essential; more important is the ability to learn and think outside the box.
Please send a CV and the name of three referees to L. D. Marks L-marks-at-northwestern.edu, email only.
-- Professor Laurence Marks Department of Materials Science and Engineering Northwestern University www.numis.northwestern.edu Corrosion in 4D: MURI4D.numis.northwestern.edu Co-Editor, Acta Cryst A "Research is to see what everybody else has seen, and to think what nobody else has thought" Albert Szent-Gyorgi
==============================Original Headers============================== 4, 26 -- From marksmsa-at-gmail.com Thu Mar 5 18:50:53 2015 4, 26 -- Received: from mail-wi0-f173.google.com (mail-wi0-f173.google.com [209.85.212.173]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t260or8a022676 4, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2015 18:50:53 -0600 4, 26 -- Received: by wibhm9 with SMTP id hm9so39847wib.2 4, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 05 Mar 2015 16:50:52 -0800 (PST) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=20120113; 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 26 -- bh=rESq0Vp9Zoex6eYrZTxUjYnSx7vsc53BBI/HbCIGqng=; 4, 26 -- b=Qc3LFWjxoka2o7Gbw6nG/MWYPzziOIyuBrpWiY0D+iBCNoPCf9POYklDoyI9Dlsxzq 4, 26 -- 5vM05aHp0stI2N64t1AgKJBq6nfhiQrI6YSiMxfdaHhwCudYQt9hwglVQ39vTACmmz5L 4, 26 -- s+fXbpjBF+7Ug3MILxxXVX43DaKarwLrkER1MdJUAA1KPER1OFzsDpR3v1lv7rizJJpH 4, 26 -- rvxB5QRLh0HW7ONIlbKyqgnRg8Z6Y/YQkHFEQBO/mGmdhQvhiLVU9jeqvVw5STaXU7ro 4, 26 -- TFruWWmsrWESJI1M4AdX8xUcBxoWZby7jyULzhAF72OU1jJbjfzdHU9zlLuM/RrmZnW/ 4, 26 -- j3SA== 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Received: by 10.180.14.7 with SMTP id l7mr70336403wic.40.1425587600135; Thu, 4, 26 -- 05 Mar 2015 12:33:20 -0800 (PST) 4, 26 -- Received: by 10.194.156.234 with HTTP; Thu, 5 Mar 2015 12:33:20 -0800 (PST) 4, 26 -- Date: Thu, 5 Mar 2015 14:33:20 -0600 4, 26 -- Message-ID: {CAHuu12otiawVsZTO67s3qhV_GeVD=0gzCZ_Jw-dOE5jzNuYqyg-at-mail.gmail.com} 4, 26 -- Subject: From Tribology to Hip Implants: Postdoctoral Position 4, 26 -- From: L Marks {marksmsa-at-gmail.com} 4, 26 -- To: microscopy {Microscopy-at-microscopy.com} 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
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Email: mattinson-at-geology.cwu.edu Name: Chris Mattinson
Organization: Central Washington University
Title-Subject: [Filtered] SEM - Recommendation on C-coater, CL detector
Message: We are looking to buy a C-coater to be used with a FE-SEM, and would be grateful for your comments/experiences with C-coaters such as Cressington 208C, Denton Desk V, Quorumtech Q150T, or others you may recommend. Our coater would primarily be used to prepare geological samples for EDS analysis and mapping, including quantitative EDS analysis using standards and beam current measurement, so the evenness and reproducibility of the coating are important.
Also, we are considering the Centaurus CL detector for the SEM, and would appreciate input from anyone who has experience with this detector.
Thanks, Chris Mattinson Central Washington University
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Email: schenderson-at-vcu.edu Name: Scott Henderson
Organization: Virginia Commonwealth University
Title-Subject: [Filtered] position available - Microscopy Technician
Message: Microscopy Technician
A technical position is available in the Microscopy Facility of the Department of Anatomy and Neurobiology in the School of Medicine at Virginia Commonwealth University. The facility houses confocal (laser scanning & spinning disc), multi-photon, TIRF, super-resolution / SIM and electron microscopes (TEM & SEM). The successful candidate will assist with microscopy studies of various biological systems. Duties include assisting users of the facility, providing basic instruction in the use of equipment within the facility (i.e. microscopes, microtomes, and image analysis programs), sample preparation (e.g. sectioning, staining), minor equipment maintenance and some administrative work (ordering of supplies and monthly billing). Applicants should have excellent communication and organizational skills, an understanding of laboratory procedures, and the ability to manage a large and varied workload. Minimum qualifications include a bachelorÂ’s degree in Science (with a concentration in Biology), previous hands-on experience with advanced light microscopy (e.g. confocal), electron microscopy, sample preparation (including sectioning), and image analysis. Computer skills are essential.
To apply, go to the VCU Jobs website at: https://www.vcujobs.com/
Click the Search Postings tab. The position number is 551220.
--------------------------
Scott Henderson, Ph.D. Director, VCU Microscopy Facility Associate Professor Dept. Anatomy & Neurobiology Virginia Commonwealth University School of Medicine 1101 East Marshall St. Richmond, VA 23298-0709
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Message: Hello, I am preparing cross-sectional TEM samples of pyroelectric materials, which crack when I am curing my epoxy. It would be great if anyone can point me towards recommended examples of room-temperature epoxies available. Thanks!
Debangshu Mukherjee PhD candidate MatSE, Penn State
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Sat Mar 7 09:58:10 2015 14, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t27FwAw7003917 14, 17 -- for {microscopy-at-microscopy.com} ; Sat, 7 Mar 2015 09:58:10 -0600 14, 17 -- Message-ID: {54FB2012.6090702-at-microscopy.com} 14, 17 -- Date: Sat, 07 Mar 2015 09:58:10 -0600 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:Room Temperature Epoxy 14, 17 -- References: {201503070641.t276fKGQ026848-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201503070641.t276fKGQ026848-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201503070641.t276fKGQ026848-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On Mar 7, 2015, at 8:35 AM, microscopylistserver- noreply-at-microscopy.com wrote:
} Name: Chris Mattinson } } Organization: Central Washington University } } Title-Subject: [Filtered] SEM - Recommendation on C-coater, CL } detector } } Message: We are looking to buy a C-coater to be used with a FE-SEM, } and would be grateful for your } comments/experiences with C-coaters such as Cressington 208C, Denton } Desk V, Quorumtech Q150T, or } others you may recommend. Our coater would primarily be used to } prepare geological samples for EDS } analysis and mapping, including quantitative EDS analysis using } standards and beam current } measurement, so the evenness and reproducibility of the coating are } important. } } Also, we are considering the Centaurus CL detector for the SEM, and } would appreciate input from } anyone who has experience with this detector. } } Thanks,
Dear Chris, I used the Cressington 208 for both carbon and metal coating, and it was very reliable and consistent. Just a satisfied customer; no financial interest. Yours, Bill
==============================Original Headers============================== 8, 32 -- From wtivol-at-sbcglobal.net Sat Mar 7 18:49:22 2015 8, 32 -- Received: from nm21-vm2.access.bullet.mail.gq1.yahoo.com (nm21-vm2.access.bullet.mail.gq1.yahoo.com [216.39.63.49]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t280nLD9013097 8, 32 -- for {microscopy-at-microscopy.com} ; Sat, 7 Mar 2015 18:49:21 -0600 8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1425775760; bh=gvZP17A01J4ymZw2T5vtdyDudnTwD79SBFxtIBsOASw=; h=From:To:In-Reply-To:Subject:Date:References:From:Subject; b=IjkZWvuqBs1KHZ0XJldoGnxo8GdfC8VVpDhuXtCkzN22MG7Bd9U7VRFksa2Go+YrAxUeyMxsS5Y2HHlI9YMBVl1dRWVwe/99UixqG7GGnVJTIf+r5Q1D1hxLAmG8gRzHPxo+VMQ0octyi+TPQRZSlHxhSeLF89E98hJKKmIdB16l17JRdzFLBy6h0FD7hbaHJzu0dm8CLwF9lQPw5SNMU4NWIgl/3LztCGxqHn43kocmpBSiPPBZjtK0h2bAhlL9ti4xOQ7MAKyy0mUtVE5mDjt6SWBbIkI5dC0VVOBNjf6bJcAH+1wKvbI+ZPaLCInI+E8+55bKwtfBgrMDjtLlAw== 8, 32 -- Received: from [216.39.60.170] by nm21.access.bullet.mail.gq1.yahoo.com with NNFMP; 08 Mar 2015 00:49:20 -0000 8, 32 -- Received: from [67.195.22.116] by tm6.access.bullet.mail.gq1.yahoo.com with NNFMP; 08 Mar 2015 00:49:20 -0000 8, 32 -- Received: from [127.0.0.1] by smtp111.sbc.mail.gq1.yahoo.com with NNFMP; 08 Mar 2015 00:49:20 -0000 8, 32 -- X-Yahoo-Newman-Id: 898186.49976.bm-at-smtp111.sbc.mail.gq1.yahoo.com 8, 32 -- X-Yahoo-Newman-Property: ymail-3 8, 32 -- X-YMail-OSG: 8gydN4UVM1m3KGqo_oYIcvjioMLLTxkjYQFqpg8LJtj57a9 8, 32 -- Ne1HMsc8xg6JsA8VGGnkNkpOeng.svuVUoU246mihGZJdCe_UNuAv2IoB4D1 8, 32 -- 43Xd01ld163HwoyB5vZABYcEjY1H.s77ZSCp3mjRfBYzUHKMZCi7_HAZyfCw 8, 32 -- 5JjWgIs.mrtY6pOeqf1swO9MnZANbVB1tIoVNYXxrZEr72Vglqk_xr0P5Ori 8, 32 -- 6FSTdyqjcldSDVIaZl2RHeO4DtSS801ja.zzqYVfA8ZLUP7nhbZcks6AX10m 8, 32 -- UrUNjV10OnOrRz_zGN9lq.nxq1kuxeRukhDKXiHYnmsBptKVhdQ3Vt.plQWy 8, 32 -- A61QW_8ANRwx3rmeEou94jLqC.EHjFksd8tXLbNvA1b07tKK7RPzM2kArlm4 8, 32 -- 8_FTOWrPX92vCAN2QWqT9XhSvAS5DtH7EOkpgq_yfWuL4JPBcpwcYC1BpQ1S 8, 32 -- 5rlwwiv2tehgSSMr9pV0qZqqt8eHHJukeOe1hrdQbFds.cf0TAxDcvAGt73D 8, 32 -- 4C7xFkNb27cjkDmhOWbvqoL.diNiaE7hlMV9lTg-- 8, 32 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 8, 32 -- Message-Id: {01B9058D-F98A-478B-9796-196B4CC067EF-at-sbcglobal.net} 8, 32 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 8, 32 -- To: microscopy-at-microscopy.com, mattinson-at-geology.cwu.edu 8, 32 -- In-Reply-To: {201503071635.t27GZSMZ015996-at-ns.microscopy.com} 8, 32 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 32 -- Content-Transfer-Encoding: 7bit 8, 32 -- Mime-Version: 1.0 (Apple Message framework v936) 8, 32 -- Subject: Re: [Microscopy] viaWWW:SEM - Recommendation on C-coater, CL detector 8, 32 -- Date: Sat, 7 Mar 2015 16:49:18 -0800 8, 32 -- References: {201503071635.t27GZSMZ015996-at-ns.microscopy.com} 8, 32 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
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Email: cmazareanu-at-yahoo.com Name: Constantin
Organization: hobby
Title-Subject: [Filtered] kevex 4855
Message: Hi I am looking for any information regarding kevex 4855 SEM control digital interface. I need setup information and and a schematic if is possible. I searched around internet but no information is available. This is a part of a larger effort to bring a SEm to digital age. I am looking also for KEVEX MCA schematics and setup. Cannot find anywhere KEvex Sigma software. There is someone who can help me? Thank you Constantin
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==============================Original Headers============================== 14, 17 -- From microscopylistserver-noreply-at-microscopy.com Sun Mar 8 17:01:19 2015 14, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t28M1INq025100 14, 17 -- for {microscopy-at-microscopy.com} ; Sun, 8 Mar 2015 17:01:18 -0500 14, 17 -- Message-ID: {54FCC6AE.7090401-at-microscopy.com} 14, 17 -- Date: Sun, 08 Mar 2015 17:01:18 -0500 14, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 14, 17 -- MIME-Version: 1.0 14, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 17 -- Subject: viaWWW:kevex 4855 info needed 14, 17 -- References: {201503081559.t28FxZej007013-at-ns.microscopy.com} 14, 17 -- In-Reply-To: {201503081559.t28FxZej007013-at-ns.microscopy.com} 14, 17 -- X-Forwarded-Message-Id: {201503081559.t28FxZej007013-at-ns.microscopy.com} 14, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We have an issue with our two conventional Gatan heating holders (inconnel and tantalum) and were wondering, if someone else faced the same.
The problem is that we cannot get HRTEM images when the holder is connected to the power supply and the supply is switched on. It doesn't matter if the heating is running or not, it just has to be switched on. The power supply is connected to a socket coming from the microscope and additional grounding doesn't help. Interchanging of the two power supplies makes no difference. Interestingly, this issue is not 100 procent reproducible, as from time to time everything is working perfectly and you see no effect at all. The microscope is a FEI Titan.
Here is a link to a few images. One taken with the power supply switched off and the related FFT, and one with the power supply switched on and the related FFT. (https://onedrive.live.com/redir?resid=507493AEB39D8528!211&authkey=!AHqWGyO oE_yhiCw&ithint=folder%2cjpg)
Did anybody face the same or has an idea about it? Any help is very much appreciated.
Thanks a lot Jens
==============================Original Headers============================== 7, 24 -- From jens.kling-at-web.de Mon Mar 9 07:23:17 2015 7, 24 -- Received: from mout.web.de (mout.web.de [212.227.15.3]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t29CNHEZ001516 7, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2015 07:23:17 -0500 7, 24 -- Received: from CENJens ([130.225.78.50]) by smtp.web.de (mrweb004) with 7, 24 -- ESMTPSA (Nemesis) id 0MNx4F-1YOcFr1pcZ-007XcV for 7, 24 -- {Microscopy-at-microscopy.com} ; Mon, 09 Mar 2015 13:23:16 +0100 7, 24 -- From: "Jens Kling" {jens.kling-at-web.de} 7, 24 -- To: {Microscopy-at-microscopy.com} 7, 24 -- Subject: TEM Gatan heating holder issue 7, 24 -- Date: Mon, 9 Mar 2015 13:23:15 +0100 7, 24 -- Message-ID: {001201d05a63$d1dd7c80$75987580$-at-web.de} 7, 24 -- MIME-Version: 1.0 7, 24 -- Content-Type: text/plain; 7, 24 -- charset="us-ascii" 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- X-Mailer: Microsoft Outlook 14.0 7, 24 -- Thread-Index: AdBaY3QbnkVpQNnKRI+AAy79f8sH+Q== 7, 24 -- Content-Language: en-us 7, 24 -- X-Provags-ID: V03:K0:bMFxPjQpRAB5zt0uiHriJv86s82zFbA8i5W6/kLldFnvuaqDi0S 7, 24 -- 1pvu600FEkpnz0mVvC2kWuqdO/Nv9qpwNXfLKYEnPmV3ymQ5/lg/9NXE9+XHVGXu1hNOwa1 7, 24 -- nG2IpsHHuNjWILMYtGTPOaeum6RX1wwzMWdZKdkI8GWOaZkiONQ8hI67Lv5vEepEKCdWNmK 7, 24 -- euL3rsnTI/ck31VcTR6uQ== 7, 24 -- X-UI-Out-Filterresults: notjunk:1; ==============================End of - Headers==============================
It could be vibrations induced in the connecting cable that are transferred into the holder. If it was electrical interference caused by insufficient or poor grounding, then I would think it wouldn't be intermittent. Try securing the cable and see if it improves the behavior. Otherwise, I would contact Gatan directly to see if they have a better idea.
Good luck, Chris
On Mon, Mar 9, 2015 at 8:35 AM, {jens.kling-at-web.de} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Everyone } } We have an issue with our two conventional Gatan heating holders (inconnel } and tantalum) and were wondering, if someone else faced the same. } } The problem is that we cannot get HRTEM images when the holder is connected } to the power supply and the supply is switched on. It doesn't matter if the } heating is running or not, it just has to be switched on. The power supply } is connected to a socket coming from the microscope and additional grounding } doesn't help. Interchanging of the two power supplies makes no difference. } Interestingly, this issue is not 100 procent reproducible, as from time to } time everything is working perfectly and you see no effect at all. The } microscope is a FEI Titan. } } Here is a link to a few images. One taken with the power supply switched off } and the related FFT, and one with the power supply switched on and the } related FFT. } (https://onedrive.live.com/redir?resid=507493AEB39D8528!211&authkey=!AHqWGyO } oE_yhiCw&ithint=folder%2cjpg) } } Did anybody face the same or has an idea about it? } Any help is very much appreciated. } } Thanks a lot } Jens } } } ==============================Original Headers============================== } 7, 24 -- From jens.kling-at-web.de Mon Mar 9 07:23:17 2015 } 7, 24 -- Received: from mout.web.de (mout.web.de [212.227.15.3]) } 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t29CNHEZ001516 } 7, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2015 07:23:17 -0500 } 7, 24 -- Received: from CENJens ([130.225.78.50]) by smtp.web.de (mrweb004) with } 7, 24 -- ESMTPSA (Nemesis) id 0MNx4F-1YOcFr1pcZ-007XcV for } 7, 24 -- {Microscopy-at-microscopy.com} ; Mon, 09 Mar 2015 13:23:16 +0100 } 7, 24 -- From: "Jens Kling" {jens.kling-at-web.de} } 7, 24 -- To: {Microscopy-at-microscopy.com} } 7, 24 -- Subject: TEM Gatan heating holder issue } 7, 24 -- Date: Mon, 9 Mar 2015 13:23:15 +0100 } 7, 24 -- Message-ID: {001201d05a63$d1dd7c80$75987580$-at-web.de} } 7, 24 -- MIME-Version: 1.0 } 7, 24 -- Content-Type: text/plain; } 7, 24 -- charset="us-ascii" } 7, 24 -- Content-Transfer-Encoding: 7bit } 7, 24 -- X-Mailer: Microsoft Outlook 14.0 } 7, 24 -- Thread-Index: AdBaY3QbnkVpQNnKRI+AAy79f8sH+Q== } 7, 24 -- Content-Language: en-us } 7, 24 -- X-Provags-ID: V03:K0:bMFxPjQpRAB5zt0uiHriJv86s82zFbA8i5W6/kLldFnvuaqDi0S } 7, 24 -- 1pvu600FEkpnz0mVvC2kWuqdO/Nv9qpwNXfLKYEnPmV3ymQ5/lg/9NXE9+XHVGXu1hNOwa1 } 7, 24 -- nG2IpsHHuNjWILMYtGTPOaeum6RX1wwzMWdZKdkI8GWOaZkiONQ8hI67Lv5vEepEKCdWNmK } 7, 24 -- euL3rsnTI/ck31VcTR6uQ== } 7, 24 -- X-UI-Out-Filterresults: notjunk:1; } ==============================End of - Headers==============================
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Email: twh-at-cen.dtu.dk Name: Thomas Hansen
Organization: DTU
Title-Subject: [Filtered] TIA on Windows 8.1
Message: Hi.
Has anyone managed to get the TIA software from FEI running on a Windows 8.1 PC?
Thanks.
Thomas
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==============================Original Headers============================== 18, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 9 08:55:53 2015 18, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t29DtrSY001518 18, 17 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2015 08:55:53 -0500 18, 17 -- Message-ID: {54FDA669.7010301-at-microscopy.com} 18, 17 -- Date: Mon, 09 Mar 2015 08:55:53 -0500 18, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 18, 17 -- MIME-Version: 1.0 18, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 17 -- Subject: viaWWW:FEI TIA on Windows 8.1 18, 17 -- References: {201503091103.t29B3tHO026729-at-ns.microscopy.com} 18, 17 -- In-Reply-To: {201503091103.t29B3tHO026729-at-ns.microscopy.com} 18, 17 -- X-Forwarded-Message-Id: {201503091103.t29B3tHO026729-at-ns.microscopy.com} 18, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The only way I was able to get TIA running was to set up a virtual machine and install WinXP in it. I used VirtualBox though there are other virtual machine programs as well.
Good luck, Henk
On 3/9/2015 9:57 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from:twh-at-cen.dtu.dk } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy bothtwh-at-cen.dtu.dk as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email:twh-at-cen.dtu.dk } Name: Thomas Hansen } } Organization: DTU } } Title-Subject: [Filtered] TIA on Windows 8.1 } } Message: Hi. } } Has anyone managed to get the TIA software from FEI running on a Windows 8.1 PC? } } Thanks. } } Thomas } } } Login Host: 130.225.78.31 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } }
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Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
The deadline for abstract submission has passed and as you are making plans to attend the Portland meetings (Aug. 2-6), please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.
The students will be paid $10 an hour to work for ~20 hours (up to 40 hours) during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia (assisting with audio-visual needs, speaker set-up, maintaining an attendance count), staffing the volunteer office, monitoring use of the Internet Café, and helping with vendor tutorial sign-up. Payment is given as a check at the end of the meetings or when the student leaves Portland.
Once the program has been finalized, each registered bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up "working" sessions they would attend anyway. There is an added bonus of $10 cash to help with meals for each morning and/or afternoon session worked and a meeting shirt.
If anyone would like to participate in the bursary program, please check the "I wish to apply for a student bursary" box in section 2 of the registration form. Also, please send me an email of your intention. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.
For those 'non-students' volunteers are also needed to help with the above mentioned meeting activities. Although not paid on an hourly basis as the student bursaries, volunteers do receive a meeting shirt and the same cash allotment to help with meals. Volunteers also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact:
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-7998 alawrence-at-i2at.msstate.edu
==============================Original Headers============================== 10, 31 -- From ALawrence-at-i2at.msstate.edu Mon Mar 9 11:01:06 2015 10, 31 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t29G16a5000750 10, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2015 11:01:06 -0500 10, 31 -- Received: from maillag01.ad.msstate.edu ([130.18.230.70]) 10, 31 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id t29G16l9004852 10, 31 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 10, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2015 11:01:06 -0500 10, 31 -- X-Sender: {} 10, 31 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by 10, 31 -- maillag01.ad.msstate.edu (2002:8212:e646::8212:e646) with Microsoft SMTP 10, 31 -- Server (TLS) id 15.0.913.22; Mon, 9 Mar 2015 11:01:03 -0500 10, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 10, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 10, 31 -- 15.00.0913.011; Mon, 9 Mar 2015 11:00:45 -0500 10, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 10, 31 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 10, 31 -- Subject: Student Bursary program - M&M 2015 Portland meeting 10, 31 -- Thread-Topic: Student Bursary program - M&M 2015 Portland meeting 10, 31 -- Thread-Index: AdBagSLtgRQoGlgRSHikeHilI2geKw== 10, 31 -- Date: Mon, 9 Mar 2015 16:00:44 +0000 10, 31 -- Message-ID: {e5c0d62c81734d96ba653aace7a38d07-at-mail02.ad.msstate.edu} 10, 31 -- Accept-Language: en-US 10, 31 -- Content-Language: en-US 10, 31 -- X-MS-Has-Attach: 10, 31 -- X-MS-TNEF-Correlator: 10, 31 -- x-originating-ip: [130.18.230.93] 10, 31 -- Content-Type: text/plain; charset="iso-8859-1" 10, 31 -- MIME-Version: 1.0 10, 31 -- Content-Transfer-Encoding: 8bit 10, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t29G16a5000750 ==============================End of - Headers==============================
I'm trying to export data from a 2D EELS spectrum image in Digital Micrograph and I¹m not sure what the best approach is. Here is my problem:
1. I have a 2D EELS map / SI of a thin film interface, where x is some width parallel to the interface, y is some width perpendicular to the interface, and z is the EELS energy range (400 700 eV). 2. I would like to integrate all the spectra in plane (x-direction) to improve signal-to-noise (This would essentially leave me with an EELS line scan parallel to y). 3. I would then like to export slices at specified integration windows normal to the plane (y-direction) to text files.
The only way I can currently do this is by drawing an ROI onto the SI, which generates an individual spectrum integrated across x. I then have to export this and drag the ROI, repeating ad nauseam until the entire y length of the scan is traversed. Is there a simpler and faster way to do this?
Please let me know if you need more clarification. Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
Hi Steven, If you use MATLAB you can import DM3 files. Here's what might be a useful link (I have not used this particular one but it looks simple): http://www.mathworks.com/matlabcentral/fileexchange/29351-dm3-import-for-gat an-digital-micrograph
ImageJ will also open .DM3 files directly; I do all my analyses in that package. There are plugins that can be used for this as well, that you can try on spectrum images.
Once you are in one of those programs you can easily write scripts to do any arbitrary operation on the data.
Good Luck! -Larry Scipioni
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: Monday, March 09, 2015 8:17 PM To: LES-at-ZSGENETICS.COM
Hello everyone,
I'm trying to export data from a 2D EELS spectrum image in Digital Micrograph and I¹m not sure what the best approach is. Here is my problem:
1. I have a 2D EELS map / SI of a thin film interface, where x is some width parallel to the interface, y is some width perpendicular to the interface, and z is the EELS energy range (400 700 eV). 2. I would like to integrate all the spectra in plane (x-direction) to improve signal-to-noise (This would essentially leave me with an EELS line scan parallel to y). 3. I would then like to export slices at specified integration windows normal to the plane (y-direction) to text files.
The only way I can currently do this is by drawing an ROI onto the SI, which generates an individual spectrum integrated across x. I then have to export this and drag the ROI, repeating ad nauseam until the entire y length of the scan is traversed. Is there a simpler and faster way to do this?
Please let me know if you need more clarification. Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
I would suggest that you use a multivariate statistical analysis (MSA) approach such as using AXSIA (Automated eXpert Spectrum Image Analysis) software or the MSA plug-in for DM that does Principal Component Analysis. The AXSIA software uses Matlab and the SIMSIMAN module that comes with the AXSIA software would allow you to extract your line profile easily. It would also be available in Matlab for any manipulation or export that you need. The MSA plug-in would allow you to reconstruct your data to improve your signal to noise for the profile. In both cases, you must be careful to align your EELS spectra in energy throughout the spectrum image using either the zero loss peak or a peak that is in both phases and does not have a chemical shift. You must also take care of eliminating X-ray peaks in your EELS data, otherwise they are identified as unique phases. If you do this, you minimize the number of factors (components) identified. Masasha Watanabe and Paul Kotula gave excellent tutorial talks at M&M'13 on the topic. Both are available online for viewing. You might have to contact John Mansfield for the link because I don't have it available as I write this. The advantage of the MSA approach is that your analysis gives you an image and so any inhomogeneities across your interface in terms of the concentration profile would easily be identified. It is also a totally unbiased analysis approach.
Masashi is the author of the MSA plugin for DM and it is available through HREM Research. Paul is a co-author of the AXSIA software and a co-patent holder for it as well. I would highly recommend that you look up their publications on the topic as they are very good reference articles to have.
We just started using the AXSIA technique after having Paul Kotula give us a tutorial at the Army Research Laboratory and are finding it a very powerful. It's a bit more trickier with EELS that with XEDS, though.
-Scott Walck
----- Original Message ----- X-from: "steven spurgeon" {steven.spurgeon-at-pnnl.gov} To: "S Walck" {S.Walck-at-comcast.net} Sent: Monday, March 9, 2015 8:17:02 PM
Hello everyone,
I'm trying to export data from a 2D EELS spectrum image in Digital Micrograph and I�m not sure what the best approach is. Here is my problem:
1. I have a 2D EELS map / SI of a thin film interface, where x is some width parallel to the interface, y is some width perpendicular to the interface, and z is the EELS energy range (400 � 700 eV). 2. I would like to integrate all the spectra in plane (x-direction) to improve signal-to-noise (This would essentially leave me with an EELS line scan parallel to y). 3. I would then like to export slices at specified integration windows normal to the plane (y-direction) to text files.
The only way I can currently do this is by drawing an ROI onto the SI, which generates an individual spectrum integrated across x. I then have to export this and drag the ROI, repeating ad nauseam until the entire y length of the scan is traversed. Is there a simpler and faster way to do this?
Please let me know if you need more clarification. Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
Steven, Digital Micrograph has a host of tools for dealing with 3-D data sets. There is a menu labeled "Volume". This will allow you to rotate or project the data along any direction needed.
For your application, you would want to project the data along the "y". You will now have a 2D dataset with the projected intensity along the interface in the X-Dimension and Energy in the Y-Dimension.
To save this as a series of files, you would then use the "File:Save As Series ..." menu item. You can choose EMSA format for the file type and the EELS header information and calibrations will be preserved. You can also use the "Text" format, then you only get the intensities.
You can write a simple script in Digital Micrograph to do this. Below is an example. It took about 4x longer to document that actually write.
For more information about scripting, there is a good reference section in the Digital Micrograph help file. You can also get a lot of information at the DM Script site at TUGraz { http://portal.tugraz.at/portal/page/portal/felmi/DM-Script }
Hope this helps, Ray
//**************** // Simple script to project a Spectrum Image into a Line Scan.
image imgSrc := GetFrontImage() // Grabs a pointer to the front dataset. This is your 3D Spectrum Image image imgRes; // Variable for result. This does not yet exist
// Get the size of the spectrum image number sX, sY, sZ; sX = imgSrc.ImageGetDimensionSize(0); sY = imgSrc.ImageGetDimensionSize(1); sZ = imgSrc.ImageGetDimensionSize(2);
// Choose a projection direction. Returns "1" for "Along X" and "0" for "Along Y". // 1/0 is interpreted as true/false (Actually 0 = false, non-zero = true) number projectX = TwoButtonDialog("Choose a projection direction", "Along X", "Along Y")
//Create the image to receive the projection // Uses the "ImageClone" function to keep all of the acquisition tags // Uses the "Slice2" function to choose a sub-region of the original data // There are more elegant ways to do this, but this is simple. if(projectX) imgRes := ImageClone(imgSrc.Slice2(0, 0, 0, 2, sZ, 1 , 1, sY, 1)); else imgRes := ImageClone(imgSrc.Slice2(0, 0, 0, 2, sZ, 1 , 0, sX, 1));
// Set the new image to all zeros. imgRes = 0;
//Do the projection. Uses the image iterators to operate on the entire image at once. This could be done // with a for loop, but with an interpreted language, it would take forever. // This is compiled on the fly and very fast.
// Sets some image information. Uses the simple "If, Then ,Else" structure ( "logical statement" ? "Do if true" : "Do if false") // You can use this structure to operate of every pixel in an image. imgRes.SetNumberNote("EELS:Acquisition:Number of frames", (projectX ? sX : sY)) imgRes.SetName(imgSrc.GetName() + "_Projection") imgRes.ShowImage() // If you do not show the image, it is killed when the script exits.
//********************
Ray D. Twesten, Ph.D. Product Manager – Analytical Instruments Gatan, Inc. Tel. +1 (925) 224-7392
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: Monday, March 09, 2015 5:08 PM To: ray.twesten-at-sbcglobal.net
Hello everyone,
I'm trying to export data from a 2D EELS spectrum image in Digital Micrograph and I¹m not sure what the best approach is. Here is my problem:
1. I have a 2D EELS map / SI of a thin film interface, where x is some width parallel to the interface, y is some width perpendicular to the interface, and z is the EELS energy range (400 700 eV). 2. I would like to integrate all the spectra in plane (x-direction) to improve signal-to-noise (This would essentially leave me with an EELS line scan parallel to y). 3. I would then like to export slices at specified integration windows normal to the plane (y-direction) to text files.
The only way I can currently do this is by drawing an ROI onto the SI, which generates an individual spectrum integrated across x. I then have to export this and drag the ROI, repeating ad nauseam until the entire y length of the scan is traversed. Is there a simpler and faster way to do this?
Please let me know if you need more clarification. Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
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Email: lauri.rimorin-at-nih.gov Name: Lauri Rimorin
Organization: Leidos Biomedical Research, Inc.
Title-Subject: [Filtered] Scientist I Opening in Frederick, MD (FNLCR)
Message: Leidos Biomedical Research, Inc. (LBRI), a wholly owned subsidiary of Leidos, operates the Frederick National Laboratory for Cancer Research (FNLCR). FNLCR is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI). It is the only FFRDC dedicated to biomedical research. Through its status as an FFRDC, FNLCR provides NCI and others with a unique national resource to accelerate the development and delivery of effective preventive, diagnostic, and therapeutic products for cancer and AIDS.
The breadth of FNLCRÂ’s activities spans the research and development spectrum, including investigator-initiated, hypothesis-driven research into cancer and AIDS; advanced technology programs focused on genetics and genomics, proteins and proteomics, imaging, nanotechnology, bioinformatics, and laboratory animal sciences; clinical operations in support of NCI and National Institute of Allergy and Infectious Diseases (NIAID)-sponsored clinical trials, as well as NCI drug discovery and development efforts; and management and operations of biopharmaceutical development and manufacturing programs under current Good Manufacturing Practice conditions for NCI and NIAID. Administrative, procurement, financial, safety, and facilities support is provided to these R&D activities through state-of-the-art business processes. LBRI has approximately 1,900 employees and manages an annual operating budget of approximately $450M.
For more information about Leidos Biomedical Research Inc., please visit our webpage at www.leidosbiomed.com.
PROGRAM DESCRIPTION
The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community. A major focus of the CRTP is the NCI RAS Initiative with the goal to discover new therapeutic interventions against RAS-driven cancers. In addition, the CRTP hosts the Nanotechnology Characterization Laboratory (NCL) which performs and standardizes preclinical efficacy and toxicity testing of nanoparticles intended for cancer therapeutics and diagnostics, and the Antibody Characterization Lab (ACL) which characterizes monoclonal antibodies or other renewable affinity binding reagents for use in cancer related research. CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.
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The Scientist I will perform: 1) negative staining and cryo-EM of proteins and protein complexes with biological sample preparation and imaging by traditional transmission electron microscopy (TEM) and scanning electron microscopy (SEM), 2) image analysis and three-dimensional reconstructions on data from negative stained specimens, cryo-electron microscopy, and electron tomography, 3) sample preparation for electron microscopy studies (negative staining, SEM, room temperature plastic embedding, cryo-electron microscopy), 4) operation of electron microscopes (SEM and TEM) and cryogenic instrumentation, 5) image analysis and three dimensional reconstructions of electron microscopy data, and 6) segmentation, interpretation and presentation of two-dimensional averages and three-dimensional volume data and fitting of high resolution data into electron density maps.
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•Possession of Doctoral degree from an accredited college or university in a field related to Computational Science, Physics, Biology or Chemistry, or eight (8) years related experience in lieu of degree •Foreign degrees must be evaluated for U.S. equivalency •Proficiency in biological and/or material sample preparation for electron microscopy •Proficiency in the use and maintenance of transmission electron microscopes and scanning electron microscopes •Strong analytical skills and ability to effectively communicate scientific results •Experience with computational image analysis of electron microscopy data (e.g. Relion, Eman, Spider, Imagic, or similar)
PREFERRED QUALIFICATIONS
•Experience with scripting or other programming skills •Experience with cryogenic liquids •Experience with maintenance of advanced instrumentation •Experience with single particle reconstruction from negative stain and/or cryo-EM data
To apply for this position, please visit http://jobs.leidos.com/ShowJob/Id/443761/Scientist-I-614205-%28NCI%29/
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When: April 27-28, 2015 Where: Norwood, Massachusetts What: Workshop on Atomic Force Microscopy to Characterize Polymers
Led by Dalia Yablon, Ph.D., this two day course mixes lecture with labwork on the basics of atomic force microscopy and its specific application to imaging polymer materials.
AFM hardware and software will be reviewed, with special emphasis on the imaging modes and image processing needed to study polymer materials.
While we utilize AFMs from AFMWorkshop to teach basic concepts and demonstrate AFM operation, attendees with experience on any make or model of atomic force microscope will find the labwork relevant and practical.
All levels of experience are welcome. An "early bird" discount registration is being offered through March, 2015.
For more information, please visit: http://www.afmworkshop.com/characterization-of-polymer-materials-afm-training.html
Thank you, Pamela Stone
Pamela Stone AFMWorkshop, Inc. 1434 E. 33rd Street Signal Hill, CA 90755 www.afmworkshop.com
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Organization: Instituto Gulbenkian de Ciência, Portugal
Title-Subject: [Filtered] TEM - Hair Samples
Message: Dear List,
We are trying to preserve newborn hair samples by HPF / AFS for TEM analysis. We are having the problem that we can not section the hair samples because the sample keeps popping out of the Epon blocks. If anyone has any experience processing hair samples, could you please contact me? I would appreciate learning how you did your infiltration, what resin you used and if you have any tricks to overcoming the problem of small samples popping out of blocks.
Thank-you for your help Erin
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Mar 12 18:23:21 2015 15, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2CNNKHf015639 15, 17 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2015 18:23:21 -0500 15, 17 -- Message-ID: {55021FE9.3010505-at-microscopy.com} 15, 17 -- Date: Thu, 12 Mar 2015 18:23:21 -0500 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:TEM - Hair Samples 15, 17 -- References: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hair and other keratin fibres are not easy tissues and must be treated differently than normal tissue samples. However, what method you need depends on if you are looking at the hair above the skin only, or if you are also looking at the hair follicle. For the moment I'll assume you are interested in only the hair above the skin.
Hair is already fixed by nature. It is also very dense. The water content is very low, and the cells are dead. It is the opposite to normal living cells in terms of the problems for TEM preparation. It can be very easy to work with also depending on what features you want to see.
The easiest method is to wash the hair to remove external lipids and dirt, place the hairs across a small frame made of plastic, or thread hairs through a narrow plastic tube, embed in LR-White (epoxy is ok too), trim, section with a diamond knife to about 100 nm/gold sections and section stain with uranyl acetate and lead citrate (slightly extended stain times compared to normal) and you can see most features.
The resin will not penetrate the hair. But the hair will sit inside the resin. There are often problems with folding (you can reduce this with thicker sections) and sometimes problems at the edges of the fibres where the fibre has swollen with the water in the knife boat while the resin has not.
If you want to see the intermediate filaments that make up most of the cortex of the hair, you have to do something more complicated involving repeated treatments of reduction to open up disulfides to attach stain to and use osmium. Or there is also a silver nitrate method which allows you to see the filaments, but at the expense of seeing various other structures.
I'll send a separate email to you with a paper that colleagues and I put together with all these methods.
Harland, D. P., Vernon, J. A., Walls, R. J., & Woods, J. L. (2011). Transmission electron microscopy staining methods for the cortex of human hair: a modified osmium method and comparison with other stains. Journal of Microscopy, 243(2), 184-196. doi: 10.1111/j.1365-2818.2011.03493.x
Kind regards Duane
____________________________ Dr Duane P Harland Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz AgResearch Limited Lincoln Research Centre Cnr Springs Rd & Gerald Street, Lincoln Private Bag 4749 Christchurch 8140, New Zealand T +64 3 325 9900 F +64 3 325 9946 www.agresearch.co.nz Farming Food and Health. First Te Ahuwhenua Te Kai me te Whai Ora. Tuatahi
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Organization: Instituto Gulbenkian de Ciência, Portugal
Title-Subject: [Filtered] TEM - Hair Samples
Message: Dear List,
We are trying to preserve newborn hair samples by HPF / AFS for TEM analysis. We are having the problem that we can not section the hair samples because the sample keeps popping out of the Epon blocks. If anyone has any experience processing hair samples, could you please contact me? I would appreciate learning how you did your infiltration, what resin you used and if you have any tricks to overcoming the problem of small samples popping out of blocks.
Thank-you for your help Erin
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Thu Mar 12 18:23:21 2015 15, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2CNNKHf015639 15, 17 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2015 18:23:21 -0500 15, 17 -- Message-ID: {55021FE9.3010505-at-microscopy.com} 15, 17 -- Date: Thu, 12 Mar 2015 18:23:21 -0500 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:TEM - Hair Samples 15, 17 -- References: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201503121423.t2CENIRI007827-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers============================== ======================================================================= Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately. =======================================================================
We¹ve recently been conducting STEM-EELS analysis of chromium oxides and we¹d like to get more precise about our quantification of white line ratios in these compounds. To that end, we¹d like to prepare Cr standards that are not oxides (to avoid overlap between the Cr L23 and O K edges). Does anyone have any recommendations for compounds that have worked well for this purpose? I am currently considering buying powders such as CrCl3, but I¹d be open to suggestions and preparation tips.
Thanks! __________________________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Fundamental and Computational Sciences Directorate Pacific Northwest National Laboratory
we are trying to observe ice in the SEM. The purpose is visualising the transition of high density ice (ice II or III) to low density ice, either ice Ic or Ih using EBSD. The cryo stage in the present setup does not get lower than -140°C and this is only just below the recrystallisation temperature.
To prevent charging gas is admitted into the microscope. This however has a tremendous effect on the stage temperature which easily goes up to -110°C, way above the recrystallisation temperature. As a result we have so far of course not been able to identify the high pressure ice polymorphs.
The best remedy would very probably be to improve the cold stage so we can reach lower temperatures, and for the future this may well be what will be done. For the time being I am looking for alternatives to admitting gas to prevent charging.
Two ideas popped up: freezing salt water or freezing a conductive nanoparticle solution, e.g. gold or silver or as was suggested by Guenter Resch carbon rods. The rationale being that in the eutectica between the pure ice crystals a high concentration of ions or nanoparticles forms a network of conductive material that might or might not assist in reducing charge build up.
Does anyone of you have experience in this area or have alternative ideas?
Thanks,
Jan Leunissen Dept Geology - University of Otago Dunedin New Zealand.
==============================Original Headers============================== 9, 31 -- From leunissen-at-aurion.nl Mon Mar 16 16:00:11 2015 9, 31 -- Received: from nm13-vm6.access.bullet.mail.bf1.yahoo.com (nm13-vm6.access.bullet.mail.bf1.yahoo.com [216.109.115.6]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2GL0AP9005972 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2015 16:00:10 -0500 9, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1426539610; bh=LbOeOHymtFJyx7REZKA3iU/601T/No4Awkby3xmJ3fI=; h=From:Subject:Date:To:From:Subject; b=AFpHWSs2ePzn5fAdXWlMup/y405G67MD2oZENfXo48XwNsW/JgMNYvsJN//7s6XKNyiZnZeDFn0XTy37FB+PnP/2LINCA8TOxkXRlKpcOExMLLgteqbsSPZn2kXV6xvCS2l8RTtsvtQ9ao3QJs4ooI8xim6RfwinwFiI9HHkqy60X2I4XSFzqEEWM6SIIdjfMxukqcSNBNDRf1MCNdfuT59vEJpxRkow+i/8q5nhOX9poyXIj/T8MRsZhb0rvDxL7LpJHNJBrspIZst6gvyFqMvWj6urQ+2R8MiipRIgBG7nI8Pl67WKfM/wE9Ugeh4Y9zMKOlAyDb0qw8ocVeFXlQ== 9, 31 -- Received: from [66.196.81.158] by nm13.access.bullet.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- Received: from [98.139.244.50] by tm4.access.bullet.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- Received: from [127.0.0.1] by smtp112.sbc.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- X-Yahoo-Newman-Id: 270704.77657.bm-at-smtp112.sbc.mail.bf1.yahoo.com 9, 31 -- X-Yahoo-Newman-Property: ymail-3 9, 31 -- X-YMail-OSG: y2Aps44VM1l2nRyUVnqPdnc5H19i9IJN80qoZcPtGyQIMSH 9, 31 -- C3WjFz7v33PxOsOVonRNZUjGr4r2FjI0CpAVFxHi0LRMO1OOINquaoBPudyE 9, 31 -- snE9gGKVZBqla05z_GNToYDwWQnRDXtIH3POgFMtrzetr4_KX4xOR_C5y8s5 9, 31 -- GI80EaPaxEiuo1Joq45_VU6Ipspvt7tlJMzWVEl6sD3pSrcXtPBpGPVmjdbr 9, 31 -- iKwgaSYLp_2VP1gqkN1Zs1WoM4dCX7IYU_PJZ06vsW0UFums_yO_bfqpgIXL 9, 31 -- OoDftKCv_kJBq2UHrAgI_Ps4CK0KoqKYCHAxJuqaEZwkrmjRTwcLvRJSKQ.R 9, 31 -- iqDSVt7ACSG_YJ6I284694ok0KUPsHCQz2XFOSDJlGwiJbJNb_3ZMdZsGQrc 9, 31 -- wZNqaVz_2VbFbDrjFCjPlbjmcaJO0pmSD8LGtBErRimgzZgjV0wRJnoVWThc 9, 31 -- J7KKe82fdQeDQwyrfgD2S76Iirky.Kg2N963MYcDjnUfQbWeEY3.AvVd_7em 9, 31 -- IQ_rFoCee4yHmBj8R0Sbt9kN98CoLpOPuBJjTGaYf3dPdEA-- 9, 31 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 9, 31 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 31 -- Content-Type: text/plain; charset=utf-8 9, 31 -- Subject: charging of ice in SEM 9, 31 -- Message-Id: {EE9118FD-D201-417F-87EB-388AF90D6781-at-aurion.nl} 9, 31 -- Date: Tue, 17 Mar 2015 10:00:05 +1300 9, 31 -- To: microscopy-at-microscopy.com 9, 31 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.6\)) 9, 31 -- X-Mailer: Apple Mail (2.2070.6) 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2GL0AP9005972 ==============================End of - Headers==============================
In my past life I ran a FE-SEM with cryostage, and charging of ice was of little issue at 1-2 kV. Most frequently, 1 or 1.5 kV. What sort of instrument are you using? Can you get a low enough kV to reach charge balance?
And ... adding nanoparticles, salt water, etc. I'd wonder about that. Yes, the crystallization process does exclude ions and such to produce the ice crystals (sea ice is really interesting because of this), but I doubt that process is 100% complete. I suspect it would be less complete with nanoparticles than it is with ions. Which means adding salts or nanoparticles will affect the properties you're trying to study. Plus, the added salts/nanoparticles are going to add electrical effects to your samples, even if they are excluded from the crystals. What do these do?
Phil
} Hello, } } we are trying to observe ice in the SEM. The purpose is visualising the transition of high density ice (ice II or III) to low density ice, either ice Ic or Ih using EBSD. } The cryo stage in the present setup does not get lower than -140°C and this is only just below the recrystallisation temperature. } } To prevent charging gas is admitted into the microscope. This however has a tremendous effect on the stage temperature which easily goes up to -110°C, way above the recrystallisation temperature. As a result we have so far of course not been able to identify the high pressure ice polymorphs. } } The best remedy would very probably be to improve the cold stage so we can reach lower temperatures, and for the future this may well be what will be done. For the time being I am looking for alternatives to admitting gas to prevent charging. } } Two ideas popped up: freezing salt water or freezing a conductive nanoparticle solution, e.g. gold or silver or as was suggested by Guenter Resch carbon rods. The rationale being that in the eutectica between the pure ice crystals a high concentration of ions or nanoparticles forms a network of conductive material that might or might not assist in reducing charge build up. } } Does anyone of you have experience in this area or have alternative ideas? } } } Thanks, } } Jan Leunissen } Dept Geology - University of Otago } Dunedin } New Zealand.
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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On Mar 16, 2015, at 2:23 PM, leunissen-at-aurion.nl wrote:
} we are trying to observe ice in the SEM. The purpose is visualising } the transition of high density ice (ice II or III) to low density } ice, either ice Ic or Ih using EBSD. } The cryo stage in the present setup does not get lower than -140°C } and this is only just below the recrystallisation temperature. } } To prevent charging gas is admitted into the microscope. This } however has a tremendous effect on the stage temperature which } easily goes up to -110°C, way above the recrystallisation } temperature. As a result we have so far of course not been able to } identify the high pressure ice polymorphs. } } The best remedy would very probably be to improve the cold stage so } we can reach lower temperatures, and for the future this may well be } what will be done. For the time being I am looking for alternatives } to admitting gas to prevent charging. } } Two ideas popped up: freezing salt water or freezing a conductive } nanoparticle solution, e.g. gold or silver or as was suggested by } Guenter Resch carbon rods. The rationale being that in the eutectica } between the pure ice crystals a high concentration of ions or } nanoparticles forms a network of conductive material that might or } might not assist in reducing charge build up. } } Does anyone of you have experience in this area or have alternative } ideas? } } } Thanks, } } Jan Leunissen } Dept Geology - University of Otago } Dunedin } New Zealand.
Dear Jan, I have no experience in SEM of ice, but from other posts to this list, I would try low-voltage SEM to balance electrons staying in the specimen with secondary electrons leaving the specimen. An additional comment is that trying to freeze salt water is likely to result in crystals of ice surrounded by molecules of salt, since most salts do not dissolve in ice. One exception, which I have also considered in order to increase the conductivity of ice, is NH4F, since both NH3 and HF can incorporate into the ice structure--the reference for this is a book called Physics of Ice, the name of the author of which I do not remember. Yours, Bill
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Thank you, everyone, for your replies. A brief summary below since not all messages were cc’ed to this list.
The idea of freezing brine or a conductive nanoparticle suspension met with scepticism since the ice will separate from the conductive components. The general recommendation was: use as low a kV as you can, it will cause less charging and the surface charge may even become positive.
Unfortunately the low kV and EBSD do not go well together.
Whereas the geology department in Dunedin has been very successful in imaging Ice Ih and getting excellent EBSD patterns from it, this can be done at much higher temperatures since recrystallisation is not an issue. The situation is more complicated for the high pressure crystalline ice polymorphs as the temperature of the ice must not be warmer than ~ -135°C.
A tough nut to crack, but an interesting one, so not giving up yet.
Thank you all, it was great to have so many replies. As you will have been able to tell from my question and the omission of essential details… I am not an SEM expert, so your help was very valuable.
Jan
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } } we are trying to observe ice in the SEM. The purpose is visualising the transition of high density ice (ice II or III) to low density ice, either ice Ic or Ih using EBSD. } The cryo stage in the present setup does not get lower than -140°C and this is only just below the recrystallisation temperature. } } To prevent charging gas is admitted into the microscope. This however has a tremendous effect on the stage temperature which easily goes up to -110°C, way above the recrystallisation temperature. As a result we have so far of course not been able to identify the high pressure ice polymorphs. } } The best remedy would very probably be to improve the cold stage so we can reach lower temperatures, and for the future this may well be what will be done. For the time being I am looking for alternatives to admitting gas to prevent charging. } } Two ideas popped up: freezing salt water or freezing a conductive nanoparticle solution, e.g. gold or silver or as was suggested by Guenter Resch carbon rods. The rationale being that in the eutectica between the pure ice crystals a high concentration of ions or nanoparticles forms a network of conductive material that might or might not assist in reducing charge build up. } } Does anyone of you have experience in this area or have alternative ideas? } } } Thanks, } } Jan Leunissen } Dept Geology - University of Otago } Dunedin } New Zealand. } } ==============================Original Headers============================== } 9, 31 -- From leunissen-at-aurion.nl Mon Mar 16 16:00:11 2015 9, 31 -- Received: from nm13-vm6.access.bullet.mail.bf1.yahoo.com (nm13-vm6.access.bullet.mail.bf1.yahoo.com [216.109.115.6]) } 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2GL0AP9005972 } 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2015 16:00:10 -0500 } 9, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1426539610; bh=LbOeOHymtFJyx7REZKA3iU/601T/No4Awkby3xmJ3fI=; h=From:Subject:Date:To:From:Subject; b=AFpHWSs2ePzn5fAdXWlMup/y405G67MD2oZENfXo48XwNsW/JgMNYvsJN//7s6XKNyiZnZeDFn0XTy37FB+PnP/2LINCA8TOxkXRlKpcOExMLLgteqbsSPZn2kXV6xvCS2l8RTtsvtQ9ao3QJs4ooI8xim6RfwinwFiI9HHkqy60X2I4XSFzqEEWM6SIIdjfMxukqcSNBNDRf1MCNdfuT59vEJpxRkow+i/8q5nhOX9poyXIj/T8MRsZhb0rvDxL7LpJHNJBrspIZst6gvyFqMvWj6urQ+2R8MiipRIgBG7nI8Pl67WKfM/wE9Ugeh4Y9zMKOlAyDb0qw8ocVeFXlQ== } 9, 31 -- Received: from [66.196.81.158] by nm13.access.bullet.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- Received: from [98.139.244.50] by tm4.access.bullet.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- Received: from [127.0.0.1] by smtp112.sbc.mail.bf1.yahoo.com with NNFMP; 16 Mar 2015 21:00:10 -0000 9, 31 -- X-Yahoo-Newman-Id: 270704.77657.bm-at-smtp112.sbc.mail.bf1.yahoo.com } 9, 31 -- X-Yahoo-Newman-Property: ymail-3 9, 31 -- X-YMail-OSG: y2Aps44VM1l2nRyUVnqPdnc5H19i9IJN80qoZcPtGyQIMSH } 9, 31 -- C3WjFz7v33PxOsOVonRNZUjGr4r2FjI0CpAVFxHi0LRMO1OOINquaoBPudyE } 9, 31 -- snE9gGKVZBqla05z_GNToYDwWQnRDXtIH3POgFMtrzetr4_KX4xOR_C5y8s5 } 9, 31 -- GI80EaPaxEiuo1Joq45_VU6Ipspvt7tlJMzWVEl6sD3pSrcXtPBpGPVmjdbr } 9, 31 -- iKwgaSYLp_2VP1gqkN1Zs1WoM4dCX7IYU_PJZ06vsW0UFums_yO_bfqpgIXL } 9, 31 -- OoDftKCv_kJBq2UHrAgI_Ps4CK0KoqKYCHAxJuqaEZwkrmjRTwcLvRJSKQ.R } 9, 31 -- iqDSVt7ACSG_YJ6I284694ok0KUPsHCQz2XFOSDJlGwiJbJNb_3ZMdZsGQrc } 9, 31 -- wZNqaVz_2VbFbDrjFCjPlbjmcaJO0pmSD8LGtBErRimgzZgjV0wRJnoVWThc } 9, 31 -- J7KKe82fdQeDQwyrfgD2S76Iirky.Kg2N963MYcDjnUfQbWeEY3.AvVd_7em } 9, 31 -- IQ_rFoCee4yHmBj8R0Sbt9kN98CoLpOPuBJjTGaYf3dPdEA-- } 9, 31 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF } 9, 31 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 31 -- Content-Type: text/plain; charset=utf-8 9, 31 -- Subject: charging of ice in SEM 9, 31 -- Message-Id: {EE9118FD-D201-417F-87EB-388AF90D6781-at-aurion.nl} } 9, 31 -- Date: Tue, 17 Mar 2015 10:00:05 +1300 9, 31 -- To: microscopy-at-microscopy.com 9, 31 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.6\)) 9, 31 -- X-Mailer: Apple Mail (2.2070.6) 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2GL0AP9005972 ==============================End of - Headers==============================
==============================Original Headers============================== 12, 37 -- From leunissen-at-aurion.nl Thu Mar 19 16:32:39 2015 12, 37 -- Received: from nm23-vm7.access.bullet.mail.bf1.yahoo.com (nm23-vm7.access.bullet.mail.bf1.yahoo.com [216.109.115.166]) 12, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2JLWchw025102 12, 37 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2015 16:32:39 -0500 12, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1426800758; bh=MTIclw8O86ckCCe93kIC29b8SYz64cAwrTQGl61/vCc=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=QgQKAssfmm3owKDJkIS14TobjlYF9eYfQ5RArDpi5hbcTRvzly1z4DxufKqqaZBpzceKrup+HkwN+ht9GKcgXi1/xWJt2un5A/PyomkWMKLWjtZ/bLEZA/MBPf2aFiEtmRIpxD3M2CN7QsTXlImygfjJLAVEzRovDomaewvwqZUVjtL7TtiOeNhhJiisSy2J7uMBaEe9382qdNclhp8BuP6osirpipNIyvOCeGDdUKBZgn6kZJZ16LfcAiYcmPG/8LaRDDJy0qt3N/8DLoIQts4QTKiHkpkP5kogHExsjpExqSB58J2HBBmLlrycLtn9LxfTo29ckYnGPbm5eYYfSw== 12, 37 -- Received: from [66.196.81.160] by nm23.access.bullet.mail.bf1.yahoo.com with NNFMP; 19 Mar 2015 21:32:38 -0000 12, 37 -- Received: from [98.139.244.51] by tm6.access.bullet.mail.bf1.yahoo.com with NNFMP; 19 Mar 2015 21:32:38 -0000 12, 37 -- Received: from [127.0.0.1] by smtp113.sbc.mail.bf1.yahoo.com with NNFMP; 19 Mar 2015 21:32:38 -0000 12, 37 -- X-Yahoo-Newman-Id: 122782.74263.bm-at-smtp113.sbc.mail.bf1.yahoo.com 12, 37 -- X-Yahoo-Newman-Property: ymail-3 12, 37 -- X-YMail-OSG: 7N1n0wQVM1n4xuLejYbY6jQasOduj6qFmHbUheGShit1dtD 12, 37 -- brN4d_bB3hAKK2ODg8cf2PIvQzt6n6wvs4xtowAxKo5QOKCGsmNQihYp1.N_ 12, 37 -- 3QT6DNNY9qm1M17ZNWtjj8OHFK1fRh0shNFMTdcYzs8XOgTh_Uj0dGWTu3zV 12, 37 -- eZnTTwJGU.9Sb_Wh7Q.Lw7_6.ca.t7L9PPSA6ARi46lXFP.e2tjARDUFRi3r 12, 37 -- e.evHcw0hT6swDU3yMgqzeRazLKc5V_dfFAZ4SNC9BJzT45H4Bc7_Kc99.2q 12, 37 -- RFWmP9c48gbCGElWDClDQ0BbJg7CeDSkVonms3tVjd_fjwVACBvxaoVybYT5 12, 37 -- DJU2yUNSrFrnsUBoBcIc6Vk0SwRU_v33r3IoIRlGhsEtS7glZHttBamV6.jJ 12, 37 -- pjjid2Z0ufnM0116K9stWtwSRQSrZ0FjhaztI48zQ9DAKrWQc5UadJiNjOiU 12, 37 -- HB_Mr3ZcVdLzWRX8Ayutty7fdCtX0U0dvXZP0vdndBNdewuX5cBfwNCkJEQe 12, 37 -- lFyG_WjT1uQ0pJns3.MhfgalLZQuXBGv7M3T4Cxxr8P9krtfgHl8vW8Bhkwy 12, 37 -- dujOnpE0YhRgj5BSFRD.LTC6KkjVYcauh85xhKd15HQ70kDg8KaaW4a4IKX8 12, 37 -- 3dUL9wPLGM2z0g277Qp3fnJPv_OXNtOIYUVfg9y0UtOhjmSJ7AWbDjYtPHFI 12, 37 -- lbh5VNFox_DPJuO0AUQGIrCM7iyvzfUU5k1elPP2XkgHC_RV8UgFUvHnqCXg 12, 37 -- 5fde_HUHIdoqEsE8eD79eMaO_dnm0F_sEzgakHxEifdME 12, 37 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 12, 37 -- Content-Type: text/plain; charset=windows-1252 12, 37 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2070.6\)) 12, 37 -- Subject: Re: [Microscopy] charging of ice in SEM - summary 12, 37 -- From: Jan Leunissen {leunissen-at-aurion.nl} 12, 37 -- In-Reply-To: {BLUPR04MB546B98149BE3D6CD680A10DD7030-at-BLUPR04MB546.namprd04.prod.outlook.com} 12, 37 -- Date: Fri, 20 Mar 2015 10:32:27 +1300 12, 37 -- Message-Id: {43497D60-7FDA-490C-8F31-607FDEDA75AA-at-aurion.nl} 12, 37 -- References: {201503162101.t2GL1eBs007492-at-ns.microscopy.com} {BLUPR04MB546B98149BE3D6CD680A10DD7030-at-BLUPR04MB546.namprd04.prod.outlook.com} 12, 37 -- To: microscopy-at-microscopy.com 12, 37 -- X-Mailer: Apple Mail (2.2070.6) 12, 37 -- Content-Transfer-Encoding: 8bit 12, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2JLWchw025102 ==============================End of - Headers==============================
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Email: m.aindow-at-uconn.edu Name: Mark Aindow
Organization: University of Connecticut
Title-Subject: [Filtered] Senior Staff Position: UConn-FEI Center of Excellence in Microscopy.
Message: Academic Assistant 4 - Institute of Materials Science
The Institute of Materials Science (IMS) at the University of Connecticut is seeking qualified applicants to fill the position of Academic Assistant IV for the newly established UConn-FEI Center of Excellence in Microscopy. The Center has been formed as a partnership between UConn and FEI Inc. to serve the analytical needs of industrial and academic researchers. The Center is acquiring seven new electron beam instruments over the next two years including state-of-the-art TEM, SEM and FIB systems. These will be housed in the UConn Technology Park as part of purpose-built Advanced Characterization Laboratory (ACL), which is scheduled to open in 2017.
Working under the direction of the Center Director, the Academic Assistant will co-ordinate, oversee, and be responsible for the effective and safe management of the existing IMS Microscopy Laboratory and the new Center of Excellence. Duties will include: supervising the staff and students who work for the Center; keeping abreast of published, scientific literature to understand what has been done before and any new developments in the field; sharing their knowledge with staff and students; publishing papers as appropriate; overseeing the operation, maintenance and user training on the instruments; managing systems for user booking and logging of instrument usage; responsible for maintenance of instruments and providing advice on instrument upgrades, including the co-ordination of new equipment installation in the IMS Microscopy Laboratory and the subsequent relocation to the ACL.
Minimum Qualifications: An earned doctorate in Materials Science, Chemistry, Physics or a related discipline; a minimum of five years post-doctoral experience involving the use of electron microscopes and related instrumentation; good written and verbal communication skills; strong interpersonal skills including the ability to interact effectively with faculty, staff, students and customers from industry.
Preferred Qualifications: Experience with FEI instruments, including: aberration-corrected TEMs, small dual-beam FIB systems and SEMs; Prior experience of microscopy user facility management; Strong track record of working with industry.
Appointment Terms: This is a full-time, 11-month position with an anticipated start date of May 1st. Salary will be commensurate with qualifications and experience.
To Apply: Interested applicants should submit a cover letter, C.V., and contact information for three references online at Husky Hire (www.jobs.uconn.edu). Review of applications will begin immediately. Position inquiries should be directed to the Institute Director, Dr. Steven Suib (steven.suib-at-uconn.edu). Employment of the successful candidate will be contingent upon the successful completion of a pre-employment background check. (Search # 2015336)
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If you have been considering sending in an application to NIH/National Heart, Lung and Blood Institute for an "Electron Microscopy Senior Scientist and/or Core Director" (see MSA Job posting ID 22114004) you only have about two more weeks to get it in.
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Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491 connellyps-at-mail.nih.gov
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Email: chrisbrantner-at-email.gwu.edu Name: Chris Brantner
Organization: George Washington University
Title-Subject: [Filtered] reticle for Leica Ultracut R
Message: Good afternoon listers,
I was wondering if anyone out there has a reticle that fits into the eye piece of a Leica Ultracut R that they are not using and would be willing to part with. I would like to put it on this ultramicrotome that I will be using to train students so that they can "see" how large their resin blackface is.
Thanks Chris
George Washington U-Center for Nanofabrication and Imaging Washington DC chrisbrantner-at-gwu.edu
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Email: klaus.leifer-at-angstrom.uu.se Name: Klaus Leifer
Organization: Uppsala University
Title-Subject: [Filtered] 2nd international workshop on ”TEM spectroscopy in materials science”
Message: Uppsala University organises the 2nd international workshop on ”TEM spectroscopy in materials science” in Uppsala (18th-20th May). We have invited very good colleagues from the field of spectroscopy and have made strong efforts to keep the registration fees very low. We believe that this could make the workshop interesting for some of your co-workers or students. Uppsala is the closest city to Stockholm airport (18min) and the airport is easy to reach for national and international flights.
Detailed Information about the workshop can be found at this URL
http://www.teknik.uu.se/elmin/spectroscopy.php
Best regards Klaus
-- Prof. Klaus Leifer, Chair of Experimental Physics, Laboratory of Electron Microscopy and Nanoengineering, Div. Applied Materials Science, Dep. Engineering Science, Uppsala University, Angstromlaboratory, Lägerhyddsv. 1, Box 534, 751 21 UPPSALA, Sweden Tel: 0046 18-471 7942, Mobile: 0046 70 425 09 81, Fax: 0046 18-471 32 70, email: klaus.leifer-at-Angstrom.uu.se
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==============================Original Headers============================== 25, 18 -- From microscopylistserver-noreply-at-microscopy.com Wed Mar 25 08:50:43 2015 25, 18 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 25, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2PDohje024544 25, 18 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2015 08:50:43 -0500 25, 18 -- Message-ID: {5512BD33.7020306-at-microscopy.com} 25, 18 -- Date: Wed, 25 Mar 2015 08:50:43 -0500 25, 18 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 25, 18 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 25, 18 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 25, 18 -- MIME-Version: 1.0 25, 18 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 25, 18 -- Subject: viaWWW:2nd international workshop on =?windows-1252?Q?=94TEM_s?= 25, 18 -- =?windows-1252?Q?pectroscopy_in_materials_science=94?= 25, 18 -- References: {201503251347.t2PDl7oX024471-at-ns.microscopy.com} 25, 18 -- In-Reply-To: {201503251347.t2PDl7oX024471-at-ns.microscopy.com} 25, 18 -- X-Forwarded-Message-Id: {201503251347.t2PDl7oX024471-at-ns.microscopy.com} 25, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 25, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We have a very valuable sample that was wrapped in Kapton tape for analysis by synchrotron and micro-CT. The problem now is that we want to remove the tape and sticky residue to prepare the sample for FIB and TEM.
Does anyone have any recipes (chemical or otherwise) for getting the polymers off cleanly? We could bake / burn the sample (it's refractory ceramic) but we don't want to do this unless necessary.
Thanks Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Radiation Effects and Microstructural Analysis Group Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 6, 37 -- From parishcm-at-ornl.gov Fri Mar 27 09:07:13 2015 6, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2RE7AcI002059 6, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2015 09:07:13 -0500 6, 37 -- X-SG: RELAYLIST 6, 37 -- X-IronPort-AV: E=Sophos;i="5.11,479,1422939600"; 6, 37 -- d="scan'208";a="77237093" 6, 37 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 6, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 27 Mar 2015 10:07:10 -0400 6, 37 -- Received: from EXCHCS31.ornl.gov (exchcs31.ornl.gov [128.219.12.145]) 6, 37 -- (using TLSv1 with cipher AES256-SHA (256/256 bits)) 6, 37 -- (No client certificate requested) 6, 37 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 31694100145 6, 37 -- for {Microscopy-at-Microscopy.Com} ; Fri, 27 Mar 2015 10:07:10 -0400 (EDT) 6, 37 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS31.ornl.gov 6, 37 -- (128.219.12.145) with Microsoft SMTP Server (TLS) id 15.0.1044.25; Fri, 27 6, 37 -- Mar 2015 10:07:09 -0400 6, 37 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by 6, 37 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id 6, 37 -- 15.00.1044.021; Fri, 27 Mar 2015 10:07:09 -0400 6, 37 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 6, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 6, 37 -- Subject: Removing Kapton tape 6, 37 -- Thread-Topic: Removing Kapton tape 6, 37 -- Thread-Index: AdBol09xycvJFFxeSWiSWHeC5oAvjw== 6, 37 -- Date: Fri, 27 Mar 2015 14:07:08 +0000 6, 37 -- Message-ID: {8601c06cd41a4b8fb03522f5764d20fb-at-EXCHCS32.ornl.gov} 6, 37 -- Accept-Language: en-US 6, 37 -- Content-Language: en-US 6, 37 -- X-MS-Has-Attach: 6, 37 -- X-MS-TNEF-Correlator: 6, 37 -- x-ms-exchange-transport-fromentityheader: Hosted 6, 37 -- x-originating-ip: [128.219.12.132] 6, 37 -- Content-Type: text/plain; charset="us-ascii" 6, 37 -- MIME-Version: 1.0 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2RE7AcI002059 ==============================End of - Headers==============================
I had good luck removing Kapton tape residue from thin (~250um) semiconductor samples by soaking in warm (~40C, covered beaker under fume hood) acetone overnight and then gently rubbing with a Q-tip soaked in acetone on a flat piece of teflon plastic.
If you find a better approach - please share.
Valery Ray - also with NISP Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
On 3/27/2015 10:09 AM, parishcm-at-ornl.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have a very valuable sample that was wrapped in Kapton tape for analysis by synchrotron and micro-CT. The problem now is that we want to remove the tape and sticky residue to prepare the sample for FIB and TEM. } } Does anyone have any recipes (chemical or otherwise) for getting the polymers off cleanly? We could bake / burn the sample (it's refractory ceramic) but we don't want to do this unless necessary. } } Thanks } Chad } } --------------------- } Chad M. Parish, Ph.D. } Research and Development Staff Member } Radiation Effects and Microstructural Analysis Group } Materials Science and Technology Division } Oak Ridge National Laboratory } Phone: 1 865 574 0092 } Email: parishcm-at-ornl.gov } } } } ==============================Original Headers============================== } 6, 37 -- From parishcm-at-ornl.gov Fri Mar 27 09:07:13 2015 } 6, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) } 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2RE7AcI002059 } 6, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2015 09:07:13 -0500 } 6, 37 -- X-SG: RELAYLIST } 6, 37 -- X-IronPort-AV: E=Sophos;i="5.11,479,1422939600"; } 6, 37 -- d="scan'208";a="77237093" } 6, 37 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) } 6, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 27 Mar 2015 10:07:10 -0400 } 6, 37 -- Received: from EXCHCS31.ornl.gov (exchcs31.ornl.gov [128.219.12.145]) } 6, 37 -- (using TLSv1 with cipher AES256-SHA (256/256 bits)) } 6, 37 -- (No client certificate requested) } 6, 37 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 31694100145 } 6, 37 -- for {Microscopy-at-Microscopy.Com} ; Fri, 27 Mar 2015 10:07:10 -0400 (EDT) } 6, 37 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS31.ornl.gov } 6, 37 -- (128.219.12.145) with Microsoft SMTP Server (TLS) id 15.0.1044.25; Fri, 27 } 6, 37 -- Mar 2015 10:07:09 -0400 } 6, 37 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by } 6, 37 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id } 6, 37 -- 15.00.1044.021; Fri, 27 Mar 2015 10:07:09 -0400 } 6, 37 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} } 6, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} } 6, 37 -- Subject: Removing Kapton tape } 6, 37 -- Thread-Topic: Removing Kapton tape } 6, 37 -- Thread-Index: AdBol09xycvJFFxeSWiSWHeC5oAvjw== } 6, 37 -- Date: Fri, 27 Mar 2015 14:07:08 +0000 } 6, 37 -- Message-ID: {8601c06cd41a4b8fb03522f5764d20fb-at-EXCHCS32.ornl.gov} } 6, 37 -- Accept-Language: en-US } 6, 37 -- Content-Language: en-US } 6, 37 -- X-MS-Has-Attach: } 6, 37 -- X-MS-TNEF-Correlator: } 6, 37 -- x-ms-exchange-transport-fromentityheader: Hosted } 6, 37 -- x-originating-ip: [128.219.12.132] } 6, 37 -- Content-Type: text/plain; charset="us-ascii" } 6, 37 -- MIME-Version: 1.0 } 6, 37 -- Content-Transfer-Encoding: 8bit } 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t2RE7AcI002059 } ==============================End of - Headers============================== }
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Email: 13qw9-at-queensu.ca Name: Qiang Wang
Organization: Queen's University
Title-Subject: [Filtered] Bruker Esprit 1.9 Problem
Message: Hi all, we just installed an Bruker Esprit 1.9 offline software. Some problems happened. The first one is when I was trying to do QMap for some existed ChemiSTEM HyperMap data, all the QMap images are just black but not with different colors as usual. The second one is when I wanted to make a new QMap method, error happened like "cliff-lorimer: wrong primary energy in standard library". So, I guess there may be some parameters need to be changed? I change the "Voltage range" to 200KV in the "Microscope information" already. So, if anyone of you know any possible reason for this, please let me know. Thank you very much!
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A friend is trying to get some instruments running but doesn't have the manual for this. I found something similar, but it would be good to have the actual user manual. Does anyone have a way to get this?
I've collected what I have so far here: http://siliconpr0n.org/wiki/doku.php?id=lecroy:6010_magic_controller
John
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Organization: West Virginia Univirsity EM Department
Title-Subject: [Filtered] Service Contracts on older equipment
Message: We have an older Leica UCT ultramicrotome that is used for research and is no longer covered under service contracts provided by Leica. Are there any companies that would provide service contracts for older equipment such as this?
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==============================Original Headers============================== 15, 17 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 30 17:52:46 2015 15, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2UMqkt6027635 15, 17 -- for {microscopy-at-microscopy.com} ; Mon, 30 Mar 2015 17:52:46 -0500 15, 17 -- Message-ID: {5519D3BE.4070003-at-microscopy.com} 15, 17 -- Date: Mon, 30 Mar 2015 17:52:46 -0500 15, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 15, 17 -- MIME-Version: 1.0 15, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 17 -- Subject: viaWWW:Service Contracts on older Leica equipment 15, 17 -- References: {201503301557.t2UFvcm7017716-at-ns.microscopy.com} 15, 17 -- In-Reply-To: {201503301557.t2UFvcm7017716-at-ns.microscopy.com} 15, 17 -- X-Forwarded-Message-Id: {201503301557.t2UFvcm7017716-at-ns.microscopy.com} 15, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Although the older microtomes are officially no longer covered by service contracts, you can often convince your Leica microtome specialist to service them. They may complain, but they will usually do it (in our experience, at least). We recently moved into a different building, and the microtome specialist gave all of the microtomes a service, even the oldest Ultracut (pre-Ultracut E). It will just cost a chunk of money.
However, in the USA, you likely have alternatives to Leica. They may not give you a service contract but I imagine they will service the instruments - they just need to be aware of the idiosyncracies of the different models.
good luck, cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 E rosemary.white-at-csiro.au
On 31/03/15 10:00 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
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Generally when you receive the message that you have a "wrong primary energy in standard library" it means that your spectra or map was recorded at a different energy than that used to build the Esprit library.
Go to the menu "Database", then top right open the button "Standard Library" and select "new". Accept to change the current library and fill the data for the new one: -any name you like, - elevation is the take-off angle, probably 18° or 22° for Titan or Osiris respectively, - azimuth the angle between the goniometer axis and the diode positions 45° - sample tilt that you used to record the data.
Regards
Philippe
==============================Original Headers============================== 5, 19 -- From philippe.buffat-at-epfl.ch Mon Mar 30 19:35:09 2015 5, 19 -- Received: from smtp5.epfl.ch (smtp5.epfl.ch [128.178.224.8]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t2V0Z98X004303 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 30 Mar 2015 19:35:09 -0500 5, 19 -- Received: (qmail 6716 invoked by uid 107); 31 Mar 2015 00:35:06 -0000 5, 19 -- X-Virus-Scanned: ClamAV 5, 19 -- Received: from vpn-254-045.epfl.ch (HELO vpn-254-045.epfl.ch) (128.179.254.45) 5, 19 -- by mail.epfl.ch (AngelmatoPhylax SMTP proxy) with ESMTP; Tue, 31 Mar 2015 02:35:06 +0200 5, 19 -- Message-ID: {5519EBB6.4090401-at-epfl.ch} 5, 19 -- Date: Tue, 31 Mar 2015 02:35:02 +0200 5, 19 -- From: Philippe Buffat {philippe.buffat-at-epfl.ch} 5, 19 -- Reply-To: philippe.buffat-at-epfl.ch 5, 19 -- Organization: EPFL 5, 19 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy-at-microscopy.com 5, 19 -- Subject: Bruker Esprit 1.9 Problem 5, 19 -- Content-Type: text/plain; charset=utf-8; format=flowed 5, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Dear All, anybody out there who can give me the original Zeiss part number of the serial interface used to remote a Zeiss DSM 960 ? It should be something like "348331-9023-1234" Or better: Somebody out there who would like to part with this interface card?
Best regards, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
We have 3 FEI FEG SEMs in our building. A new FEI FIB, a 4 yr old FEI nanoSEM and a 15 yr old FEI XL30 SFEG
We have 2 older sputter coaters. A Polaron E5100 (1988) and a Denton Desk II. Both use mech pumps. Our Polaron is no longer working
The problem of course is the visible grain at high mags (} 50Kx) with gold, gold/palladium, etc. It doesn't matter if it's the Polaron or the Denton. Same result. Visible grain over 50Kx.
I need to justify with written rationale to our directors why we should consider buying a high res coater. Simply stating "to compliment our 3 FEG SEMs" is not enough of a reason to spend the money. They prefer I fix the Polaron.
Any ideas/feedback from those who have already crossed this bridge would be helpful.
Thank you in advance
Also, does anyone have a resource for parts for Polaron coaters circa 1988 models?
Fred Hayes Manager, AMCaT Labs CHMS, College of Eng UC Davis
==============================Original Headers============================== 12, 51 -- From fahayes-at-ucdavis.edu Tue Mar 31 21:02:02 2015 12, 51 -- Received: from smtp2.ucdavis.edu (smtp2.ucdavis.edu [128.120.32.219]) 12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t312211I009135 12, 51 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2015 21:02:02 -0500 12, 51 -- Received: from exhub.ex.ad3.ucdavis.edu (mailbag.housing.ucdavis.edu [169.237.229.112]) 12, 51 -- by smtp2.ucdavis.edu (8.14.4/8.14.5/it-oel6-mimedefang-smtp-1.9) with ESMTP id t3121mde006125 12, 51 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=OK) 12, 51 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2015 19:02:00 -0700 12, 51 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (207.46.163.184) 12, 51 -- by exhub.ex.ad3.ucdavis.edu (169.237.229.112) with Microsoft SMTP Server 12, 51 -- (TLS) id 14.3.224.2; Tue, 31 Mar 2015 19:01:58 -0700 12, 51 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com (10.242.127.155) by 12, 51 -- DM2PR0801MB651.namprd08.prod.outlook.com (10.242.127.154) with Microsoft SMTP 12, 51 -- Server (TLS) id 15.1.118.21; Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) by 12, 51 -- DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) with mapi id 12, 51 -- 15.01.0118.022; Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 12, 51 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 51 -- Subject: rationale for buying a high res sputter coater 12, 51 -- Thread-Topic: rationale for buying a high res sputter coater 12, 51 -- Thread-Index: AdBsHbeDSWYu9NZsSriicsUEbzfugg== 12, 51 -- Date: Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- Message-ID: {DM2PR0801MB652387327D70F18ECE1AC91BEF30-at-DM2PR0801MB652.namprd08.prod.outlook.com} 12, 51 -- Accept-Language: en-US 12, 51 -- Content-Language: en-US 12, 51 -- X-MS-Has-Attach: 12, 51 -- X-MS-TNEF-Correlator: 12, 51 -- x-originating-ip: [169.237.64.26] 12, 51 -- authentication-results: microscopy.com; dkim=none (message not signed) 12, 51 -- header.d=none; 12, 51 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB651; 12, 51 -- x-forefront-antispam-report: BMV:1;SFV:NSPM;SFS:(10009020)(6009001)(504964003)(102836002)(50986999)(54356999)(40100003)(86362001)(87936001)(450100001)(76576001)(2501003)(2656002)(122556002)(77156002)(92566002)(2900100001)(2351001)(229853001)(62966003)(77096005)(90282001)(33656002)(74316001)(107886001)(46102003)(110136001)(99286002)(88552001)(75432002)(89122001);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR0801MB651;H:DM2PR0801MB652.namprd08.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 12, 51 -- x-microsoft-antispam-prvs: {DM2PR0801MB651C26D2D1ADE9C9B84F910BEF30-at-DM2PR0801MB651.namprd08.prod.outlook.com} 12, 51 -- x-exchange-antispam-report-test: UriScan:; 12, 51 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5002010)(5005006);SRVR:DM2PR0801MB651;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB651; 12, 51 -- x-forefront-prvs: 053315510E 12, 51 -- Content-Type: text/plain; charset="us-ascii" 12, 51 -- MIME-Version: 1.0 12, 51 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 01 Apr 2015 02:01:56.1550 12, 51 -- (UTC) 12, 51 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 12, 51 -- X-MS-Exchange-CrossTenant-id: a8046f64-66c0-4f00-9046-c8daf92ff62b 12, 51 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR0801MB651 12, 51 -- X-OriginatorOrg: ucdavis.edu 12, 51 -- X-Greylist: Sender succeeded STARTTLS authentication, not delayed by milter-greylist-4.4.3 (smtp2.ucdavis.edu [128.120.32.8]); Tue, 31 Mar 2015 19:02:00 -0700 (PDT) 12, 51 -- X-Virus-Scanned: clamav-milter 0.98.1 at smtp2 12, 51 -- X-Virus-Status: Clean 12, 51 -- X-Scanned-By: MIMEDefang 2.74 12, 51 -- Content-Transfer-Encoding: 8bit 12, 51 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t312211I009135 ==============================End of - Headers==============================
At the risk of sounding too salsesman-y, I'd like to offer my thoughts on your situation.
First, congrats on having 3 FEGs at your facility! That's always a good problem to have! A couple things to consider:
As for the coaters, I would start with the following approach. First, figure out the size of the features you are looking for. You should have an idea of the grain size put down by each of your coaters. If the coating thickness or grain size exceeds the size of the features you are looking for, you will never see them. I would try stating it in such a way that you may be missing important information or obtaining inaccurate information from your samples because it is highly possible small features have been completely obscured by the thickness and or grain size of the metal coating you are currently using. I would recommend either an osmium coater or a high vacuum iridium or platinum coater, as you will be able to lay down much thinner coatings with grain sizes that may not even be visible.
If you are working at very low accelerating voltages, know that almost all samples, regardless of how carefully they were prepared or stored, build up a thin layer of hydrocarbon material on the surface. When working below 2kV, this contamination contributes significantly to the image formed. Ideally it should be removed with a UV cleaning cycle (although a very low power plasma clean may work on some materials). Doing so may will give a much better, and more accurate surface image, and may eliminate the need for a metal coating in some instances.
In the end, I think a valid way to frame your request is by stating that you want to use the equipment and tools that will get you the most accurate data from your samples, and not leave your results open to questioning or second guessing. If it results in saving time (samples come out right the first time) or money (new systems come with warranties, less prone to breakdowns) that might also help.
Best of luck! If you would like to continue this conversation offline, I would be happy to.
Cheers,
Jeff
jhall-at-2spi.com
Jeffrey A. Hall | Microscopist SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA 1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook
Dismclaimer: SPI Supplies manufactures and distributes tools for people working in microscopy laboratories
________________________________________ X-from: fahayes-at-ucdavis.edu {fahayes-at-ucdavis.edu} Sent: Tuesday, March 31, 2015 10:19 PM To: Jeff Hall
Listers,
We have 3 FEI FEG SEMs in our building. A new FEI FIB, a 4 yr old FEI nanoSEM and a 15 yr old FEI XL30 SFEG
We have 2 older sputter coaters. A Polaron E5100 (1988) and a Denton Desk II. Both use mech pumps. Our Polaron is no longer working
The problem of course is the visible grain at high mags (} 50Kx) with gold, gold/palladium, etc. It doesn't matter if it's the Polaron or the Denton. Same result. Visible grain over 50Kx.
I need to justify with written rationale to our directors why we should consider buying a high res coater. Simply stating "to compliment our 3 FEG SEMs" is not enough of a reason to spend the money. They prefer I fix the Polaron.
Any ideas/feedback from those who have already crossed this bridge would be helpful.
Thank you in advance
Also, does anyone have a resource for parts for Polaron coaters circa 1988 models?
Fred Hayes Manager, AMCaT Labs CHMS, College of Eng UC Davis
==============================Original Headers============================== 12, 51 -- From fahayes-at-ucdavis.edu Tue Mar 31 21:02:02 2015 12, 51 -- Received: from smtp2.ucdavis.edu (smtp2.ucdavis.edu [128.120.32.219]) 12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t312211I009135 12, 51 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2015 21:02:02 -0500 12, 51 -- Received: from exhub.ex.ad3.ucdavis.edu (mailbag.housing.ucdavis.edu [169.237.229.112]) 12, 51 -- by smtp2.ucdavis.edu (8.14.4/8.14.5/it-oel6-mimedefang-smtp-1.9) with ESMTP id t3121mde006125 12, 51 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=OK) 12, 51 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2015 19:02:00 -0700 12, 51 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (207.46.163.184) 12, 51 -- by exhub.ex.ad3.ucdavis.edu (169.237.229.112) with Microsoft SMTP Server 12, 51 -- (TLS) id 14.3.224.2; Tue, 31 Mar 2015 19:01:58 -0700 12, 51 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com (10.242.127.155) by 12, 51 -- DM2PR0801MB651.namprd08.prod.outlook.com (10.242.127.154) with Microsoft SMTP 12, 51 -- Server (TLS) id 15.1.118.21; Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- Received: from DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) by 12, 51 -- DM2PR0801MB652.namprd08.prod.outlook.com ([10.242.127.155]) with mapi id 12, 51 -- 15.01.0118.022; Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 12, 51 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 51 -- Subject: rationale for buying a high res sputter coater 12, 51 -- Thread-Topic: rationale for buying a high res sputter coater 12, 51 -- Thread-Index: AdBsHbeDSWYu9NZsSriicsUEbzfugg== 12, 51 -- Date: Wed, 1 Apr 2015 02:01:56 +0000 12, 51 -- Message-ID: {DM2PR0801MB652387327D70F18ECE1AC91BEF30-at-DM2PR0801MB652.namprd08.prod.outlook.com} 12, 51 -- Accept-Language: en-US 12, 51 -- Content-Language: en-US 12, 51 -- X-MS-Has-Attach: 12, 51 -- X-MS-TNEF-Correlator: 12, 51 -- x-originating-ip: [169.237.64.26] 12, 51 -- authentication-results: microscopy.com; dkim=none (message not signed) 12, 51 -- header.d=none; 12, 51 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB651; 12, 51 -- x-forefront-antispam-report: BMV:1;SFV:NSPM;SFS:(10009020)(6009001)(504964003)(102836002)(50986999)(54356999)(40100003)(86362001)(87936001)(450100001)(76576001)(2501003)(2656002)(122556002)(77156002)(92566002)(2900100001)(2351001)(229853001)(62966003)(77096005)(90282001)(33656002)(74316001)(107886001)(46102003)(110136001)(99286002)(88552001)(75432002)(89122001);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR0801MB651;H:DM2PR0801MB652.namprd08.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 12, 51 -- x-microsoft-antispam-prvs: {DM2PR0801MB651C26D2D1ADE9C9B84F910BEF30-at-DM2PR0801MB651.namprd08.prod.outlook.com} 12, 51 -- x-exchange-antispam-report-test: UriScan:; 12, 51 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5002010)(5005006);SRVR:DM2PR0801MB651;BCL:0;PCL:0;RULEID:;SRVR:DM2PR0801MB651; 12, 51 -- x-forefront-prvs: 053315510E 12, 51 -- Content-Type: text/plain; charset="us-ascii" 12, 51 -- MIME-Version: 1.0 12, 51 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 01 Apr 2015 02:01:56.1550 12, 51 -- (UTC) 12, 51 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 12, 51 -- X-MS-Exchange-CrossTenant-id: a8046f64-66c0-4f00-9046-c8daf92ff62b 12, 51 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR0801MB651 12, 51 -- X-OriginatorOrg: ucdavis.edu 12, 51 -- X-Greylist: Sender succeeded STARTTLS authentication, not delayed by milter-greylist-4.4.3 (smtp2.ucdavis.edu [128.120.32.8]); Tue, 31 Mar 2015 19:02:00 -0700 (PDT) 12, 51 -- X-Virus-Scanned: clamav-milter 0.98.1 at smtp2 12, 51 -- X-Virus-Status: Clean 12, 51 -- X-Scanned-By: MIMEDefang 2.74 12, 51 -- Content-Transfer-Encoding: 8bit 12, 51 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t312211I009135 ==============================End of - Headers==============================
==============================Original Headers============================== 29, 28 -- From jhall-at-2spi.com Wed Apr 1 07:36:28 2015 29, 28 -- Received: from SPI-EX-01.spi.local (mail.2spi.com [209.120.197.107]) 29, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t31CaSv0015083 29, 28 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 07:36:28 -0500 29, 28 -- Received: from SPI-EX-01.spi.local (172.25.75.31) by SPI-EX-01.spi.local 29, 28 -- (172.25.75.31) with Microsoft SMTP Server (TLS) id 15.0.847.32; Wed, 1 Apr 29, 28 -- 2015 08:35:35 -0400 29, 28 -- Received: from SPI-EX-01.spi.local ([fe80::f8c4:9b23:d8de:fa70]) by 29, 28 -- SPI-EX-01.spi.local ([fe80::f8c4:9b23:d8de:fa70%12]) with mapi id 29, 28 -- 15.00.0847.030; Wed, 1 Apr 2015 08:35:35 -0400 29, 28 -- From: Jeff Hall {jhall-at-2spi.com} 29, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 29, 28 -- Subject: RE: [Microscopy] rationale for buying a high res sputter coater 29, 28 -- Thread-Topic: [Microscopy] rationale for buying a high res sputter coater 29, 28 -- Thread-Index: AQHQbCI+vtFjTwF650qdZXTql9qpgZ04ESZK 29, 28 -- Date: Wed, 1 Apr 2015 12:35:35 +0000 29, 28 -- Message-ID: {dc3081fa7e3c40dfa7d25d179b1d350c-at-SPI-EX-01.spi.local} 29, 28 -- References: {201504010219.t312JaHa022597-at-ns.microscopy.com} 29, 28 -- In-Reply-To: {201504010219.t312JaHa022597-at-ns.microscopy.com} 29, 28 -- Accept-Language: en-US 29, 28 -- Content-Language: en-US 29, 28 -- X-MS-Has-Attach: 29, 28 -- X-MS-TNEF-Correlator: 29, 28 -- x-originating-ip: [172.25.75.111] 29, 28 -- Content-Type: text/plain; charset="us-ascii" 29, 28 -- MIME-Version: 1.0 29, 28 -- Content-Transfer-Encoding: 8bit 29, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t31CaSv0015083 ==============================End of - Headers==============================
The University of Bath is holding a one-day Microscopy and Analysis Conference on 6th May 2015.
We have some excellent speakers for the Conference - see http://blogs.bath.ac.uk/mas/ for details of speakers, information about the trade exhibition and a conference program to download.
There will be a free buffet lunch and wine/beer reception for delegates!
It is free to sign up for the conference - just email Ursula Potter at University of Bath (u.j.potter-at-bath.ac.uk).
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Email: rhsia-at-umaryland.edu Name: Ru-ching Hsia
Organization: University of Maryland, Baltimore
Title-Subject: [Filtered] 2015 UMB Current Electron Microscopy Techniques Workshop
Message: Dear Colleagues,
We are pleased to announce that the Electron Microscopy Core Imaging facility (EMCIF) at the University of Maryland Baltimore will be hosting the Second Annual Current EM Techniques Workshop on May 28th and 29th, 2015.
The focus of this yearÂ’s workshop is immuno electron microscopy. The workshop will include oral presentations and tips-and-tricks discussion forums during the morning session followed by live instrument demonstrations and hands-on practice in the afternoon session.
Dr. Kent McDonald will be keynote speaker this year. There will be four tips and trick discussion forum sessions led by experienced panelists to discuss various aspects of immuno EM methods and techniques. Demo instruments will feature high pressure freezer, freeze substitution systems, a cryo-ultramicrotome, a grid plunger, cryo-TEM, cryo-SEM and possibly a CLEM system.
A dinner event will be co-sponsored by the Chesapeake Microscopy and Microanalysis society (CMMS) on May 28th providing opportunities for social and scientific exchange among workshop participants and CMMS members. More information regarding the workshop and registration can be found on our website
Please email coreimaging-at-umaryland.edu for any enquiries about the workshop.
We look forward to welcoming you in Baltimore in May.
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Email: dan.fairweather-at-delphi.com Name: Dan Fairweather
Organization: Delphi Automotive, Powertrain Div
Title-Subject: [Filtered] Diagnosing problems with a carbon coater
Message: Our lab had an EMS 450 carbon coater sitting on the counter top. I located the roughing pump in storage and am trying to make the system operational. All electrical operation seems to be working. I dumped out the old pump oil and replaced with new oil. Upon first pumping down the system, the vacuum gauge leveled at 5x10-1 mbar. I worked with the vacuum pump connections and was able to obtain 2x10-1 mbar. I ordered new seals for the jar that forms the vacuum chamber [the old seals are at least 10yrs old]. The new seals just came in, and there was no improvement to the level of vacuum. One of the observations that I have made is that I obtain the best vacuum when I turn on the system in the morning. If I leave the system running, the vacuum level will steadily worsen, holding finally at 5x10-1 mbar. Once I have cycled the system, I can never reach that level again unless I wait until the next day.
Any suggestions from the community? Do I have a pump problem or a vacuum gauge problem?
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Email: aheiss-at-amnh.org Name: Aaron Heiss
Organization: American Museum of Natural History
Title-Subject: [Filtered] TEM - MT2 Ultramicrotome Operation
Message: Hello all! I am a moderately experienced ultramicrotomist who learned on a Leica UC7, and subsequently used a Reichert-Jung Ultracut E. I'm now working with a Sorvall MT2, and while the unit is well-maintained and I can get good sections from it, I'm not sure exactly how thick it's cutting.* I know that the "main" setting is done with the knob on the top (which is marked for thickness in tens-of-Angstroms, better known as nanometres) but that this is affected by the wheel on the left of the front panel, and I'm not really sure how, or what to make of the markings (0 to 18). So, my question is this: how exactly does one set a precise thickness on the MT2?
* Yes, I can use interference colours, or check the thickness of folds, but this only measures the thickness of the sections, not how much material was cut from the block. In other words, it doesn't account for compression, which is of course variable both before and after spreading (I use chloroform vapours for this). The amount cut from the block is important because I'm doing 3-D reconstruction of serial sections, which means that I need to know exactly how far apart the sections are in the block.
TIA! Aaron
Aaron A. Heiss, Ph.D. Gerstner Scholar and Lerner-Gray Fellow Eunsoo Kim Laboratory Department of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York, NY 10024 USA
212-769-5838 aheiss-at-amnh.org
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Email: joseph.n.madary.civ-at-mail.mil Name: nick madary
Organization: joint pathology center(US Gov)
Title-Subject: [Filtered] Need to purchase a TEM with EDS any suggestions?
Message: I am sorry if this as a duplicate. In the market for a TEM with EDS and top notch imaging. If anyone has any suggestions on what not to buy and there are vendors out there please help. We do strictly biologicals(but do have a need for elemental analysis some times). regards, Nick
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==============================Original Headers============================== 11, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Apr 1 18:01:40 2015 11, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t31N1ePZ007852 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 18:01:40 -0500 11, 17 -- Message-ID: {551C78D4.6070207-at-microscopy.com} 11, 17 -- Date: Wed, 01 Apr 2015 18:01:40 -0500 11, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.5.0 11, 17 -- MIME-Version: 1.0 11, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 17 -- Subject: viaWWW:Need to purchase a TEM with EDS any suggestions? 11, 17 -- References: {201504011940.t31JesEE001574-at-ns.microscopy.com} 11, 17 -- In-Reply-To: {201504011940.t31JesEE001574-at-ns.microscopy.com} 11, 17 -- X-Forwarded-Message-Id: {201504011940.t31JesEE001574-at-ns.microscopy.com} 11, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I'm liking my Hitachi HT7700 all digital (no film) TEM. It strikes me as being very suitable for pathology. Hitachi would probably be glad to put you in contact with a couple of path labs I know of who bought one. EDS is available for that TEM, but I do not have it.
Aloha, Tina
} } Email: joseph.n.madary.civ-at-mail.mil } Name: nick madary } } Organization: joint pathology center(US Gov) } } Title-Subject: [Filtered] Need to purchase a TEM with EDS any suggestions? } } Message: I am sorry if this as a duplicate. In the market for a TEM with } EDS and top notch imaging. } If anyone has any suggestions on what not to buy and there are vendors out } there please help. We do } strictly biologicals(but do have a need for elemental analysis some times). } regards, Nick } } Login Host: 143.85.107.18 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original } Headers============================== } 11, 17 -- From microscopylistserver-noreply-at-microscopy.com Wed Apr 1 } 18:01:40 2015 } 11, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com } [206.69.208.22]) } 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } t31N1ePZ007852 } 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 18:01:40 } -0500 } 11, 17 -- Message-ID: {551C78D4.6070207-at-microscopy.com} } 11, 17 -- Date: Wed, 01 Apr 2015 18:01:40 -0500 } 11, 17 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 11, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 11, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) } Gecko/20100101 Thunderbird/31.5.0 } 11, 17 -- MIME-Version: 1.0 } 11, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 17 -- Subject: viaWWW:Need to purchase a TEM with EDS any suggestions? } 11, 17 -- References: {201504011940.t31JesEE001574-at-ns.microscopy.com} } 11, 17 -- In-Reply-To: {201504011940.t31JesEE001574-at-ns.microscopy.com} } 11, 17 -- X-Forwarded-Message-Id: } {201504011940.t31JesEE001574-at-ns.microscopy.com} } 11, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 17 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 21 -- From tina-at-pbrc.hawaii.edu Wed Apr 1 19:27:30 2015 7, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t320RUPd021941 7, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 19:27:30 -0500 7, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id t320RTFI012672 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 7, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 14:27:29 -1000 (HST) 7, 21 -- Received: from localhost (tina-at-localhost) 7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id t320RTGg012668 7, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Apr 2015 14:27:29 -1000 (HST) 7, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 7, 21 -- Date: Wed, 1 Apr 2015 14:27:29 -1000 (HST) 7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 21 -- X-X-Sender: tina-at-b1000 7, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 21 -- Subject: Re: [Microscopy] viaWWW:Need to purchase a TEM with EDS any suggestions? 7, 21 -- (fwd) 7, 21 -- Message-ID: {Pine.GSO.4.64.1504011426550.7593-at-b1000} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Plenty of variables to consider, but perhaps most straight-forward is that if the seals in your roughing pump are as old as the jar seals AND it was sitting around with used oil in it, you probably should consider a pump rebuild since there's no telling how bad things might be inside the pump. You might also check out the literature on your pump if any is available to see what vacuum levels it's capable of when working perfectly so that you can determine a real target vacuum to aim for (not knowing the details of the pump, for all we know you're actually within spec, poor as it might be).
-John
On April 1, 2015 7:26:26 PM EDT,microscopylistserver-noreply-at-microscopy.com wrote: --| --| --| --|---------------------------------------------------------------------------- --|The Microscopy ListServer -- CoSponsor: The Microscopy Society of --|America
Do you have the manual? If not, I can send a pdf of it. Basically, the knob on top is thickness in Ångstroms, and the thickness wheel on the left is a multiplier of the knob value. So: knob at 100 X wheel = thickness in Ångstroms How accurate this is depends on how well maintained and how worn is your MT-2. Particularly the Nylon block that rests on the advance screw.
Phil
} Email: aheiss-at-amnh.org } Name: Aaron Heiss } } Organization: American Museum of Natural History } } Title-Subject: [Filtered] TEM - MT2 Ultramicrotome Operation } } Message: Hello all! I am a moderately experienced ultramicrotomist who learned on a Leica UC7, and } subsequently used a Reichert-Jung Ultracut E. I'm now working with a Sorvall MT2, and while the } unit is well-maintained and I can get good sections from it, I'm not sure exactly how thick it's } cutting.* I know that the "main" setting is done with the knob on the top (which is marked for } thickness in tens-of-Angstroms, better known as nanometres) but that this is affected by the wheel } on the left of the front panel, and I'm not really sure how, or what to make of the markings (0 to } 18). So, my question is this: how exactly does one set a precise thickness on the MT2? } } * Yes, I can use interference colours, or check the thickness of folds, but this only measures the } thickness of the sections, not how much material was cut from the block. In other words, it doesn't } account for compression, which is of course variable both before and after spreading (I use } chloroform vapours for this). The amount cut from the block is important because I'm doing 3-D } reconstruction of serial sections, which means that I need to know exactly how far apart the } sections are in the block. } } TIA! } Aaron } } } Aaron A. Heiss, Ph.D. } Gerstner Scholar and Lerner-Gray Fellow } Eunsoo Kim Laboratory } Department of Invertebrate Zoology } American Museum of Natural History } Central Park West at 79th Street } New York, NY 10024 USA } } 212-769-5838 } aheiss-at-amnh.org -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 32 -- From oshel1pe-at-cmich.edu Thu Apr 2 08:56:50 2015 4, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t32DungD025412 4, 32 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2015 08:56:50 -0500 4, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 4, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id t32DuZUc008144; 4, 32 -- Thu, 2 Apr 2015 09:56:48 -0400 4, 32 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 4, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 4, 32 -- (TLS) id 14.3.195.1; Thu, 2 Apr 2015 09:56:40 -0400 4, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by 4, 32 -- CAS3.central.cmich.local (141.209.15.90) with Microsoft SMTP Server (TLS) id 4, 32 -- 14.3.195.1; Thu, 2 Apr 2015 09:56:39 -0400 4, 32 -- Message-ID: {551D4A97.5070609-at-cmich.edu} 4, 32 -- Date: Thu, 2 Apr 2015 09:56:39 -0400 4, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 32 -- MIME-Version: 1.0 4, 32 -- To: {aheiss-at-amnh.org} , micro {microscopy-at-microscopy.com} 4, 32 -- Subject: Re: [Microscopy] viaWWW:TEM - MT2 Ultramicrotome Operation 4, 32 -- References: {201504012330.t31NUYjN008255-at-ns.microscopy.com} 4, 32 -- In-Reply-To: {201504012330.t31NUYjN008255-at-ns.microscopy.com} 4, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 32 -- Content-Transfer-Encoding: 8bit 4, 32 -- X-Originating-IP: [141.209.2.100] 4, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 32 -- X-Spam-Score: -1.50 () [Hold at 6.00] RDNS_NONE,_L_IAA,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 4, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.5978; longitude=-84.7675; http://maps.google.com/maps?q=43.5978,-84.7675&z=6 4, 32 -- X-CanItPRO-Stream: default 4, 32 -- X-Canit-Stats-ID: 02Ob1UMkC - 05044bef603b - 20150402 4, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Dan's right - There are a lot of variables to consider. I'll add a few more :)
The fact that your vacuum worsens as time elapses makes me wonder if it is backstreaming oil into your carbon coater. A quick check of the vacuum line should let you know. If it is, that's the first thing to take care of before you contaminate the whole system with oil.
Assuming there is no backstreaming occurring, if you have access to a vacuum meter, you might want to attach it directly to the pump and see what kind of vacuum the pump is pulling on its own (no coater, no vacuum tubing). That should tell you which side the problem is on. If you don't have a vacuum meter, try to find a second pump to try out on the system to confirm the vacuum you can pull.
If it's the pump, a rebuild or a new pump is probably the best option. My personal experience with rebuilds has been about 50/50, for what it's worth.
If the pump seems fine and the problem seems to be on the coater side, I would start by removing the bell jar and plugging the vacuum inlet in the chamber with a stopper to see again which side the problem is on - the chamber itself, or the internals. From there, it becomes a matter of trying to check seals to find the leak.
I hope that helps - best of luck!
Jeff
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Dan,
Plenty of variables to consider, but perhaps most straight-forward is that if the seals in your roughing pump are as old as the jar seals AND it was sitting around with used oil in it, you probably should consider a pump rebuild since there's no telling how bad things might be inside the pump. You might also check out the literature on your pump if any is available to see what vacuum levels it's capable of when working perfectly so that you can determine a real target vacuum to aim for (not knowing the details of the pump, for all we know you're actually within spec, poor as it might be).
-John
On April 1, 2015 7:26:26 PM EDT,microscopylistserver-noreply-at-microscopy.com wrote: --| --| --| --|--------------------------------------------------------------------- --|------- The Microscopy ListServer -- CoSponsor: The Microscopy --|Society of America To Subscribe/Unsubscribe -- --|http://www.microscopy.com/MicroscopyListserver --|On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html --|--------------------------------------------------------------------- --|------- --| --|X-from:dan.fairweather-at-delphi.com --| --|This Question/Comment was submitted to the Microscopy Listserver --|using the WWW based Form athttp://www.microscopy.com/MLFormMail.html --|--------------------------------------------------------------------- --|------ Remember this posting is most likely not from a Subscriber, so --|when replying --|please copy bothdan.fairweather-at-delphi.com as well as the --|Microscopy Listserver --|--------------------------------------------------------------------- --|------ --| --|Email:dan.fairweather-at-delphi.com --|Name: Dan Fairweather --| --|Organization: Delphi Automotive, Powertrain Div --| --|Title-Subject: [Filtered] Diagnosing problems with a carbon coater --| --|Message: Our lab had an EMS 450 carbon coater sitting on the counter --|top. I located the roughing pump in storage and am trying to make the --|system operational. All electrical operation seems to be working. I --|dumped out the old pump oil and replaced with new oil. Upon first --|pumping down the system, the vacuum gauge leveled at 5x10-1 mbar. I --|worked with the vacuum pump connections and was able to obtain 2x10-1 --|mbar. I ordered new seals for the jar that forms the vacuum chamber --|[the old seals are at least 10yrs old]. The new seals just came in, --|and there was no improvement to the level of vacuum. One of the --|observations that I have made is that I obtain the best vacuum when I --|turn on the system in the morning. If I leave the system running, the --|vacuum level will steadily worsen, holding finally at 5x10-1 mbar. --|Once I have cycled the system, I can never reach that level again --|unless I wait until the next day. --| --|Any suggestions from the community? Do I have a pump problem or a --|vacuum gauge problem? --| --| Login Host: 216.82.243.84 --| Listserver Email Form V - 20120416 --|--------------------------------------------------------------------- --|------ --| --| --|-- --|=========================================== --|Do not reply to this message it is from the Microscopy Listserver --|NO-REPLY forwarding system. You should send a new message to --| --|Microscopy-at-Microscopy.com --| --|============================================
Hi Phil -- I don't think we do have a manual -- and whether or not we do, a PDF would be most welcome!
I should've mentioned in my original post that I'd been told that the two numbers were multiplied, but I was beginning to suspect that I'd misunderstood. This is because I'd set the top knob to my desired thickness (50 nm in this case) and the front wheel to 1, and was getting no advance at all. Setting the front wheel to 2 gave different results depending on whether I'd dialed up or down to get there. Others have told me that the best practice is to set the top knob to something much lower and dial the front wheel up -- so for 50 nm I should set the top knob to 10 and the front wheel to 5, or vice versa. I'll give that a try and see if it works.
Many thanks, to you and to everyone else who responded via e-mail!! Aaron
Aaron A. Heiss, Ph.D. Gerstner Scholar and Lerner-Gray Fellow Eunsoo Kim Laboratory Department of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York, NY 10024 USA
212-769-5838 aheiss-at-amnh.org
________________________________________ X-from: Philip Oshel [oshel1pe-at-cmich.edu] Sent: April 2, 2015 9:56 AM To: Aaron Heiss; micro
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA
Title-Subject: [Filtered] Denton DCP-1 critical point dryer gasket
Message: I got replacement gaskets for the pressure chamber from Denton because the existing one was very old (cracking, leaking). Popped the new one on and threw the old in the trash. The new ones looked thicker than the old but I chalked it up to age...
Now when I bleed gas into the chamber the gasket pops out of place no matter how tight I screw down the clamp. Mercifully at a low pressure { 50 psi.
Our instrument is pretty old, prob from 1972. I got the sense that the new ones have a groove that the o-ring sits in...
Can anyone verify this? Anyone know of a source for a slightly thinner O-ring that will fit??
thanks
Joe
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Email: Pruessner-at-ukzn.ac.za Name: Karin Pruessner
Organization: University of KwaZulu-Natal
Title-Subject: [Filtered] Postdoctoral Research Position
Message: The University of KwaZulu-Natal in beautiful Durban, South Africa, is home to an interdisciplinary Nanotechnology Platform consisting of 4 pillars in Nano Energy, Nano Materials, Nano Health and Quantum. The Nano Energy pillar has a position available for a Postdoctoral Researcher to be filled as soon as possible. Our team consists of Chemists, Physicists, Materials Scientists, and Engineers. We are working on the development of an off-grid refrigeration unit for rural areas in Africa. We are looking for a Materials Scientist with a solid background in Materials Characterization to join us. Neighboring fields will be considered. The successful candidate should have experience in nanotechnology and at least one of the following areas: • Nano Photovoltaics • Nano Energy Storage • Nano Cooling Fluids • Graphene Hands-on experience in X-Ray Diffraction, Scanning and Transmission Electron Microscopy and Raman Spectroscopy is expected. Additional expertise in Materials Synthesis would be advantageous. Interested candidates should send their electronic application materials to: Dr Karin Pruessner, Coordinator Nano Energy School of Chemistry and Physics University of KwaZulu-Natal - Westville Campus University Road Durban, 4000 South Africa Ph: ++27 31 260-7660 e-mail: Pruessner-at-ukzn.ac.za
The post is initially for one year with the possibility of renewal. Review of applications will begin immediately.
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Email: gary-at-gaugler.com Name: Dr. Gary Gaugler
Organization: Here and there
Title-Subject: [Filtered] Bruker EDS system
Message: Does anyone know about this Bruker EDS system and what its used price might be? I assume that it is an SDD. I'm not familiar with Bruker. Details of the system are below.
Bruker EDS detector
X-Flash Detector 3001: 10 mm2, FWHM { 129 Quantax single processing unit SVE Quantax workstation class computer Quantax IO-scan system and interface Esprit integrated manual
Software for Bruker EDS: Esprit Spectrum based Esprit Quant Esprit E-quant Esprit HS-quant Esprit Scan Esprit Line Esprit Map Esprit Multi-point Esprit Project Esprit Report Esprit SEMlink Esprit U-quant
What is an SVE?
TIA, gary g.
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==============================Original Headers============================== 20, 17 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 4 17:08:20 2015 20, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t34M8K5Y003547 20, 17 -- for {microscopy-at-microscopy.com} ; Sat, 4 Apr 2015 17:08:20 -0500 20, 17 -- Message-ID: {552060D3.3050901-at-microscopy.com} 20, 17 -- Date: Sat, 04 Apr 2015 17:08:19 -0500 20, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 20, 17 -- MIME-Version: 1.0 20, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 17 -- Subject: viaWWW:Bruker EDS system info needed 20, 17 -- References: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- In-Reply-To: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- X-Forwarded-Message-Id: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, may i ask if any of you have the chance to try both Gatan Cryo/ LN2 Cold Stage Holder Model 636 and CHDT3504? Are both holder performing equally well? Any pros and cons?
Cheers, Yee Yan, Tay Nanyang Technological University
==============================Original Headers============================== 2, 28 -- From rongchigram79-at-yahoo.com.sg Sun Apr 5 09:35:48 2015 2, 28 -- Received: from nm24-vm3.bullet.mail.sg3.yahoo.com (nm24-vm3.bullet.mail.sg3.yahoo.com [106.10.151.82]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t35EZkXO005663 2, 28 -- for {Microscopy-at-microscopy.com} ; Sun, 5 Apr 2015 09:35:47 -0500 2, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1428241690; bh=v5del0jaRsrAATLocxaRLbJtBlX4YGHLd8Prx6xaFj8=; h=Date:From:Reply-To:To:Subject:From:Subject; b=Z+gO8CYg7aOkxzR+ITbTLNDuvpy2P12ikqL0eBq0+8PPocizuC5neSCZiI8scOprfNhc+d0jfmp74fMbYNub9juXdnsvEduvhC+nTUuMdKWgOZ4KOvvENWYBxSJAQ3RySa2sZTlthVcY0IFL9NqfABY3IRqDNEZzLRJgvPIdTBfkMiVnHI3xJMJs7ZAtHfHXPCuUPHkzGupFTQo+LBXlFILnmrmpe8SYJ8Z3dDtu1hEWophBQmQe6WZknPIy8oMxYsN6xybHQCOvP64/XRWufQhoruShv6ogmNYzOs5qZ/8mQaVJERWio4MfVtd69K7ExxFlsRZIFK57Hir51jumWg== 2, 28 -- Received: from [106.10.166.120] by nm24.bullet.mail.sg3.yahoo.com with NNFMP; 05 Apr 2015 13:48:10 -0000 2, 28 -- Received: from [106.10.151.250] by tm9.bullet.mail.sg3.yahoo.com with NNFMP; 05 Apr 2015 13:48:10 -0000 2, 28 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com with NNFMP; 05 Apr 2015 13:48:10 -0000 2, 28 -- X-Yahoo-Newman-Property: ymail-3 2, 28 -- X-Yahoo-Newman-Id: 1396.74919.bm-at-omp1021.mail.sg3.yahoo.com 2, 28 -- X-YMail-OSG: hDuiypAVM1ndP5yD74mEkK3Q0WpV3gWvvQmqoZ8cPZlQGCCM_sGHDj3zDwQzcB0 2, 28 -- nM3v.My8j9PGoybqOYDFooDOMFy.DbZarcuffh.DSSDFDV.6LsrhifrOlPfN6JrRwRJzywI5I40s 2, 28 -- MksSFgeTEf3ulelpafM0iIW9YuhtQhNrG5baVI5VmnV1AYiOYD.nGB1eqrZgkvf49PnDNPfH5OOL 2, 28 -- ZWFaBYsIIocpkFWQ.vkdQkpIY.G7EBvJULwiPW6WBTqBiDxQ.o2z58cN3aaxj.0AB8mTvCHue510 2, 28 -- ub5a1ifSIBcdlwCZCjPdTQ6TD2Q_o3CS1b6sq3ZfiYD9CoAS3J3Vf2xsCvOwCMMfMGueZxo0E.pp 2, 28 -- jbMxv4qy3cCr8qxAuFjVBvgkALdAMhaqnml8faxMGTjgPNmHqqzsg81j59u5qOJcMghcGi9fU_0e 2, 28 -- wXHOmXTPe9XIrL8S154MVyTViyBIzv5JXlA8ZWlHGOTfxue0JdjkJw1jw75gUahxzCQ-- 2, 28 -- Received: by 106.10.196.93; Sun, 05 Apr 2015 13:48:09 +0000 2, 28 -- Date: Sun, 5 Apr 2015 13:48:09 +0000 (UTC) 2, 28 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 2, 28 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 2, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 28 -- Message-ID: {2018643352.32987.1428241689237.JavaMail.yahoo-at-mail.yahoo.com} 2, 28 -- Subject: Anyone have tried both Gatan Cryo/ LN2 Cold Stage Holder Model 636 2, 28 -- and CHDT3504 2, 28 -- MIME-Version: 1.0 2, 28 -- Content-Type: text/plain; charset=UTF-8 2, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Gary, My lab has two Bruker EDS systems and they are great. In my opinion they are the best EDS systems on the market. Yes it has a silicon drift detector. I don't know what SVE stands for. 10mm2 window is small but will likely give you more counts than you are used to (50K to 100K cps). The software options are all the most basic ones.
The big thing that is missing is the HyperMap option, which in my opinion is one of the best things about the system. It allows you to go back to a map you collected a year ago and change the element list or create an EDS spectrum of a point or area on that map just like the sample were still in the SEM. But I think Bruker will let you add that or any other software option that you may want which is not already activated.
My guess is that the system is worth at least $15K to $20K depending on its age and condition. The guy you want to talk to about this system is Robert Brandon of Bruker (Robert.Brandom-at-bruker-axs.com). He is one of their sales managers and has been very helpful to me in the past. Ted Juzwak, Applications Lab Manager at Bruker (Ted.Juzwak-at-bruker-axs.com) is also a wealth of information on these systems.
You don't see X-Flash systems on the used equipment market very often though. They last a long time and are so good no one sells them to upgrade to a better system because there isn't anything better.
Good luck,
John Knowles President MicroVision Laboratories
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Saturday, April 04, 2015 6:31 PM To: John-at-microvisionlabs.com
X-from: gary-at-gaugler.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-gaugler.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-gaugler.com Name: Dr. Gary Gaugler
Organization: Here and there
Title-Subject: [Filtered] Bruker EDS system
Message: Does anyone know about this Bruker EDS system and what its used price might be? I assume that it is an SDD. I'm not familiar with Bruker. Details of the system are below.
Bruker EDS detector
X-Flash Detector 3001: 10 mm2, FWHM { 129 Quantax single processing unit SVE Quantax workstation class computer Quantax IO-scan system and interface Esprit integrated manual
Software for Bruker EDS: Esprit Spectrum based Esprit Quant Esprit E-quant Esprit HS-quant Esprit Scan Esprit Line Esprit Map Esprit Multi-point Esprit Project Esprit Report Esprit SEMlink Esprit U-quant
What is an SVE?
TIA, gary g.
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==============================Original Headers============================== 20, 17 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 4 17:08:20 2015 20, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t34M8K5Y003547 20, 17 -- for {microscopy-at-microscopy.com} ; Sat, 4 Apr 2015 17:08:20 -0500 20, 17 -- Message-ID: {552060D3.3050901-at-microscopy.com} 20, 17 -- Date: Sat, 04 Apr 2015 17:08:19 -0500 20, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 20, 17 -- MIME-Version: 1.0 20, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 17 -- Subject: viaWWW:Bruker EDS system info needed 20, 17 -- References: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- In-Reply-To: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- X-Forwarded-Message-Id: {201504042203.t34M3FVU002936-at-ns.microscopy.com} 20, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 33, 28 -- From John-at-MicroVisionLabs.com Mon Apr 6 10:00:44 2015 33, 28 -- Received: from n55.mail01.mtsvc.net (mailout90.mail01.mtsvc.net [216.70.64.171]) 33, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t36F0iIe019801 33, 28 -- for {Microscopy-at-Microscopy.com} ; Mon, 6 Apr 2015 10:00:44 -0500 33, 28 -- Received: from c-24-61-207-227.hsd1.ma.comcast.net ([24.61.207.227]:37713 helo=JohnPC) 33, 28 -- by n55.mail01.mtsvc.net with esmtpa (Exim 4.72) 33, 28 -- (envelope-from {John-at-MicroVisionLabs.com} ) 33, 28 -- id 1Yf8W3-0002uC-Jh 33, 28 -- for Microscopy-at-Microscopy.com; Mon, 06 Apr 2015 11:00:44 -0400 33, 28 -- Reply-To: {John-at-MicroVisionLabs.com} 33, 28 -- From: "John Knowles" {John-at-MicroVisionLabs.com} 33, 28 -- To: {Microscopy-at-Microscopy.com} 33, 28 -- References: {201504042231.t34MVPWs010352-at-ns.microscopy.com} 33, 28 -- In-Reply-To: 33, 28 -- Subject: FW: [Microscopy] viaWWW:Bruker EDS system info needed 33, 28 -- Date: Mon, 6 Apr 2015 11:00:43 -0400 33, 28 -- Organization: MicroVision Labs, Inc. 33, 28 -- Message-ID: {016901d0707a$74774940$5d65dbc0$-at-MicroVisionLabs.com} 33, 28 -- MIME-Version: 1.0 33, 28 -- Content-Type: text/plain; 33, 28 -- charset="iso-8859-1" 33, 28 -- X-Mailer: Microsoft Outlook 14.0 33, 28 -- Thread-Index: AQI1z5VinkqMwXrQBMg+oxLBk+qp0Zx1SBZQgAANc+A= 33, 28 -- Content-Language: en-us 33, 28 -- X-Authenticated-User: 1235518 John-at-MicroVisionLabs.com 33, 28 -- X-MT-ID: 5872FFFCEE07D69BCD65B86159E3C59E569EAD28 33, 28 -- Content-Transfer-Encoding: 8bit 33, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t36F0iIe019801 ==============================End of - Headers==============================
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Email: scottserata-at-gmail.com Name: Scott Serata
Organization: California Academy of Sciences
Title-Subject: [Filtered] which SEM should we buy for biological/zoological work?
Message: Hi, I am the SEM engineer at the California Academy of Sciences in San Francisco. We have had a Zeiss/LEO 1450VP SEM for about 14 years. We are a zoological research organization with specialists in Entomology, Arachnology, Botany, Invertebrate Zoology, Herpetology, etc. I am starting the process of collecting information in order to purchase a new SEM. Any advice out there from people who do similar work?
Also if anyone has any feedback about the quality of field service from Zeiss, Hitachi, JEOL, FEI, etc.
Our SEM is generally used for imaging specimens using the conventional high-vacuum SE detector. Usually the specimens are coated using a gold/palladium sputter coater. We do need the ability to image uncoated specimens using either VPSE (variable pressure) or BSD. Our biggest problem with the old LEO 1450VP was the inability to get good sharp images on uncoated specimens due to charging. We generally do not need to look at wet specimens so we do not need very high chamber pressures. Currently I am looking at the Zeiss EVO MA 10 and the Hitachi SU 3500. We do not need any analytical detectors (no Xray EDS, etc) Max magnifications are perhaps 100kX. Tungsten filament electron gun is fine. We probably don't want to spend more than $200k. This is a big deal for us because we will probably be stuck with this machine for another 14 years so we want to make the right decision!
Thank you for your time! Scott Serata
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==============================Original Headers============================== 17, 17 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 7 19:33:33 2015 17, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t380XX96025279 17, 17 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2015 19:33:33 -0500 17, 17 -- Message-ID: {5524775D.9030700-at-microscopy.com} 17, 17 -- Date: Tue, 07 Apr 2015 19:33:33 -0500 17, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 17, 17 -- MIME-Version: 1.0 17, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 17 -- Subject: viaWWW:which SEM should we buy for biological/zoological work? 17, 17 -- References: {201504072044.t37Ki9Hq009904-at-ns.microscopy.com} 17, 17 -- In-Reply-To: {201504072044.t37Ki9Hq009904-at-ns.microscopy.com} 17, 17 -- X-Forwarded-Message-Id: {201504072044.t37Ki9Hq009904-at-ns.microscopy.com} 17, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: joseph.n.madary.civ-at-mail.mil Name: Nick Madary
Organization: joint pathology center
Title-Subject: [Filtered] Reply about SEM purchase
Message: Hi Scott, You have come to the right place because I have had so much great info regarding a TEM c EDS purchase. I will tell you actually using Hitachis, they are really good, I lean to the 3500 just because of my apps. JEOL has a nice table top model that is excellent as wel, you almost do not even realize there is an SEM there, the computer screen is as large as the unit it seems. You are in an enviable position. I see you have money, but if not, there are some gov surplus sites that might have really decent scopes that were turned in due to BRAC. We will do one soon ourselves. Zeiss has always been awesome at TEM,so if you can choose go with Hitachit or even that nice, new small JEOL.
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Electron microprobe Cameca model Camebax MB1 with Tracor Northern ver. TN2000 electronic control console. Max. accelerating voltage of 45 kV Fitted with BSE detector, light microscope and three WDX spectrometers: Spectrometer 1 fitted with LIF, PET, TAP, and OdPb crystals Spectrometer 2 - PET and TAP Spectrometer 3 -LIF and PET Turbomolecular vacuum pump. Documentation and some spare parts are available.
Krassimir Bozhilov
bozhilov-at-ucr.edu
==============================Original Headers============================== 6, 34 -- From krassimir.bozhilov-at-ucr.edu Thu Apr 9 15:17:13 2015 6, 34 -- Received: from mx2.ucr.edu (mx2.ucr.edu [138.23.62.3]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t39KHCRS027756 6, 34 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2015 15:17:13 -0500 6, 34 -- X-IronPort-Anti-Spam-Filtered: true 6, 34 -- X-IronPort-Anti-Spam-Result: A2CFAAD23SZVnED4F4pcg1pcBcYih0pMAQEBAQEBEgEBAQEBCAsJCRQuhCY6UQE+QicEE4gqBQioIqE+AYUBAR+OEYFXg12BFgWvboQRb4FEfwEBAQ 6, 34 -- X-IPAS-Result: A2CFAAD23SZVnED4F4pcg1pcBcYih0pMAQEBAQEBEgEBAQEBCAsJCRQuhCY6UQE+QicEE4gqBQioIqE+AYUBAR+OEYFXg12BFgWvboQRb4FEfwEBAQ 6, 34 -- X-IronPort-AV: E=Sophos;i="5.11,552,1422950400"; 6, 34 -- d="scan'208";a="365523087" 6, 34 -- Received: from exch-edge-2.ucr.edu ([138.23.248.64]) 6, 34 -- by smtp2.ucr.edu with ESMTP/TLS/AES128-SHA; 09 Apr 2015 13:17:06 -0700 6, 34 -- Received: from EXCH-CLNT-4.exch.ucr.edu (138.23.249.31) by exch-edge-2.ucr.edu 6, 34 -- (138.23.248.64) with Microsoft SMTP Server (TLS) id 14.3.224.2; Thu, 9 Apr 6, 34 -- 2015 13:16:42 -0700 6, 34 -- Received: from EXCH-MBOX-6.exch.ucr.edu ([169.254.6.194]) by 6, 34 -- exch-clnt-4.exch.ucr.edu ([138.23.249.31]) with mapi id 14.03.0224.002; Thu, 6, 34 -- 9 Apr 2015 13:17:05 -0700 6, 34 -- From: Krassimir Bozhilov {krassimir.bozhilov-at-ucr.edu} 6, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 34 -- Subject: Cameca Camebax MB1 available 6, 34 -- Thread-Topic: Cameca Camebax MB1 available 6, 34 -- Thread-Index: AQHQcwIlMMuFu1pEOEWRxSUPRuKk0g== 6, 34 -- Date: Thu, 9 Apr 2015 20:17:04 +0000 6, 34 -- Message-ID: {162245AD-7BB7-4228-A150-A297AC2D0244-at-ucr.edu} 6, 34 -- Accept-Language: en-US 6, 34 -- Content-Language: en-US 6, 34 -- X-MS-Has-Attach: 6, 34 -- X-MS-TNEF-Correlator: 6, 34 -- x-originating-ip: [138.23.63.83] 6, 34 -- Content-Type: text/plain; charset="us-ascii" 6, 34 -- Content-ID: {685C9C7010304647949395D07A28C43E-at-exch.ucr.edu} 6, 34 -- MIME-Version: 1.0 6, 34 -- Content-Transfer-Encoding: 8bit 6, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t39KHCRS027756 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Preparing gold samples for TEM
Message: Does anyone have experienced preparing gold samples for use in a TEM. I have FIB sections but there seems to be gallium from the milling present on the surface. Contact me if you would like to see the image. I understand that gold is very soft and keeping a natural texture is problematic. Any advise would be great.
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Immunogold labeling
Message: I want to label toxin A & B in C. Difficile bacterial with immunogold, so which antigen retrieval solution should I use or how to unmask the antigen before proceeding Immuno labeling on LR whte sections. Is there any universal or general method is available to unmask antigen or it should be antigen specific.?
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The Southeastern North Carolina Regional Microanalytical and Imaging Center (SENCR-MIC) at Fayetteville State University (FSU) is seeking applications for a Research Associate staff position, working primarily on a world-class Field-Emission Electron Probe Microanalyzer (EPMA) and a Scanning Electron Microscope (SEM), and other analytical instruments including AFM, XRD. The initial position is for two years, while extension is possible contingent on the performance and funding.
The successful candidate will be responsible for the day to day operations and maintenance of instruments at SENCR-MIC under the supervision of the SENCR-MIC Director. Major duties include training diverse internal and external users on the instruments, providing technical assistance to users, conducting professional services based on user fees, teaching student labs for formal courses, and other duties assigned by the Director.
Requirements: Minimum of Master's Degree (Ph.D. preferred) in Geosciences, Materials Science, Engineering, Chemistry, Physics or other related disciplines. Experiences in EPMA or SEM analysis are required, and experiences in AFM, XRD and TEM are optimum. The successful candidate must be highly dedicated to professional services, possess outstanding oral and written communication skills, and must be able to work with a wide range of users including faculty, staff, visiting scientists and students under supervision of the Director.
Application to this position should be made online at https://jobs.uncfsu.edu/postings/10917.
Fayetteville State University is committed to equality of educational opportunity and employment and does not discriminate against applicants, students, or employees based on race, color, national origin, religion, sex, gender identity, sexual orientation, age, disability, genetic information or veteran status. Moreover, Fayetteville State University values diversity and actively seeks to recruit talented students, faculty, and staff from diverse backgrounds.
==============================Original Headers============================== 9, 21 -- From zhiping_luo-at-hotmail.com Thu Apr 9 20:15:08 2015 9, 21 -- Received: from BAY004-OMC3S11.hotmail.com (bay004-omc3s11.hotmail.com [65.54.190.149]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3A1F8hf011157 9, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2015 20:15:08 -0500 9, 21 -- Received: from BAY175-W42 ([65.54.190.189]) by BAY004-OMC3S11.hotmail.com over TLS secured channel with Microsoft SMTPSVC(7.5.7601.22751); 9, 21 -- Thu, 9 Apr 2015 18:15:08 -0700 9, 21 -- X-TMN: [t+fFSseJThmdmZKkcyoI+VFmot7hgSqG] 9, 21 -- X-Originating-Email: [zhiping_luo-at-hotmail.com] 9, 21 -- Message-ID: {BAY175-W4206A1E7DD768A955C5EB08DFA0-at-phx.gbl} 9, 21 -- Reply-To: {zhiping_luo-at-hotmail.com} 9, 21 -- From: Zhiping LUO {zhiping_luo-at-hotmail.com} 9, 21 -- To: Microscopy {microscopy-at-microscopy.com} 9, 21 -- Subject: Research Associate Staff Position (mainly in EPMA) available at 9, 21 -- Fayetteville State University, North Carolina 9, 21 -- Date: Thu, 9 Apr 2015 20:15:08 -0500 9, 21 -- Importance: Normal 9, 21 -- Content-Type: text/plain; charset="iso-8859-1" 9, 21 -- MIME-Version: 1.0 9, 21 -- X-OriginalArrivalTime: 10 Apr 2015 01:15:08.0106 (UTC) FILETIME=[C8A922A0:01D0732B] 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3A1F8hf011157 ==============================End of - Headers==============================
McCrone Research Institute cordially invites you to participate in the 67th annual Inter/Micro conference by giving a presentation of your research paper. We are accepting presentation abstracts in techniques and instrumentation, environmental and industrial microscopy, and chemical and forensic microscopy. Presentations will be held at McCrone Research Institute on June 8-10.
The deadline to submit titles and abstracts is April 13, 2015. Click here to register.
Speakers will receive a $50 conference registration discount!
http://www.mcri.org/v/101/InterMicro
Tony
......................................... Andrew Anthony "Tony" Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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I am keeping alive (non-commercially) FIB-620 dual-beam FIB/SEM, which is based on the platform of Phillips XL-30/XL-40 SEM and used for student training and research projects that can't be done on brand-new instruments.
To keep it going I am looking for following parts:
If anyone will be disposing off old XL-30 or XL-40 SEM, or FIB-620 FIB I'd gladly accept it as "donation"
Thanks everyone beforehand,
-- Valery Ray - also with AIM/NISP Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
==============================Original Headers============================== 7, 30 -- From vray-at-partbeamsystech.com Fri Apr 10 10:08:22 2015 7, 30 -- Received: from nm23-vm1.bullet.mail.bf1.yahoo.com (nm23-vm1.bullet.mail.bf1.yahoo.com [98.139.213.141]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3AF8L3B006924 7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 10 Apr 2015 10:08:22 -0500 7, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1428678501; bh=Y/uGYBFJAcWJksdL8ZlBCdYVWFPQ9U5+CDm9FLF+aA0=; h=Date:From:To:Subject:From:Subject; b=MF4klSlMJbNLfQcNfCOFhDMwEdcMhhXK1SyI/NRknW9thm2Zhd49cPPsj+Z3F93LszvOj6QozBUKPRaYQNfnTkmIMZqcY9eM1EjPE6U9DBX9GuB8birN3W21T7SXbsgJdsVoMwVA+gczA3hiW7ae+wF1Qo9nz/bNLck6ffMdcFiB9C+nwKCifaC/P6+TzyXnw5YekfiuAtp3wp6NiMq+MR12t+m083J75KHHRaMOHUncttyj63ppKp/H1ykyz0N2Lsc+FoZxOGs8fhyz6LsXy825bIrBvhWG9/8LjxmG73IwX5HjrRIOISPYsHJD+vwl1WbEc52tRU8N5HvitzQVJg== 7, 30 -- Received: from [98.139.170.178] by nm23.bullet.mail.bf1.yahoo.com with NNFMP; 10 Apr 2015 15:08:21 -0000 7, 30 -- Received: from [68.142.230.75] by tm21.bullet.mail.bf1.yahoo.com with NNFMP; 10 Apr 2015 15:08:21 -0000 7, 30 -- Received: from [127.0.0.1] by smtp232.mail.bf1.yahoo.com with NNFMP; 10 Apr 2015 15:08:21 -0000 7, 30 -- X-Yahoo-Newman-Id: 590739.3789.bm-at-smtp232.mail.bf1.yahoo.com 7, 30 -- X-Yahoo-Newman-Property: ymail-3 7, 30 -- X-YMail-OSG: _iAg.fgVM1lAB0eiIBN85x8heelkWFLEO9yU1.SqD5c6Xic 7, 30 -- hXOK8EZTyhMXvavmDtczGJx9HtVfBelmGVt5TYmOTnTDlg5zjc21HXLZk_z7 7, 30 -- Soa_HjsgU76ziaypbiT8e898bB0lrKJ_TM9frQVe8yQg2iAyMGfK22bEAt8l 7, 30 -- ODAxe2bDcN63Sl2vPkFAZoyAd944tTce1fAAlc8PJFRNjuRvN51KAGGLqYvu 7, 30 -- 0Nt6ULCzm3u.6w7QSahBW_lStkf2HZkjFAKnRk5PslE515WLJ0pQreZeLoIQ 7, 30 -- SHKvmyBkSIwhw.V2iJedktRzvWgK0B8An6g9.n0eFWn.3HTjmBkOLJgQuDyb 7, 30 -- PSlf1yZE6DLNAvfb4p1mjmGIsDxbE0E8HvJOo6MKRpb0f3dYbNQxp5HTFZv9 7, 30 -- 8lixAxXrcB4sf8maAXk4StcLEWIuf0PbK34Bk.cgPDTXLLAGr5JoLAO78pR3 7, 30 -- WbbvvV_Np4m0ROuINWjw.OOTwRHeARQGtyyphbW1GZvenXvfd_cl_aU5jQKn 7, 30 -- X0dRXZUU2W4TyjOnk3Xr8exY4207QviwqVcWgrbXn 7, 30 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 30 -- Message-ID: {5527E763.8040705-at-partbeamsystech.com} 7, 30 -- Date: Fri, 10 Apr 2015 11:08:19 -0400 7, 30 -- From: Valery Ray {vray-at-partbeamsystech.com} 7, 30 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 7, 30 -- MIME-Version: 1.0 7, 30 -- To: microscopy-at-microscopy.com 7, 30 -- Subject: Looking for parts or parts or parts tool: XL-30, XL-40, or FIB-620 7, 30 -- Content-Type: text/plain; charset=utf-8; format=flowed 7, 30 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: Univ of Connecticut
Title-Subject: [Filtered] Access to User Facilities
Message: I am looking for advice from the community about how access to microscope facilities is granted to users. Specifically, I am thinking of something not quite so formal as the General User Access Proposal process available at National Labs, but not as open as our existing "free-for-all" where anyone who requests training can have open access.
I don't need advice on how to train users, rather, I need a fair process on how to determine who should or should not become a user.
Does anyone have a policy/process they wish to share--off line if you like--that weighs user requests? Perhaps a policy that includes various levels of access to users based on their needs and skills?
And a follow-up question is the magic "silver bullet" of how to monitor users after training to ensure that they are actually doing everything the way they were taught.
Thanks, Roger Ristau Univ of Connecticut
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Please send out a summary of replies once you get some. They can remain anonymous as far as I care.
We are probably a small enough lab that we have remained rather informal about access.
There are a few people that I have thought about redirecting away from the microscope as their brain just doesn't seem to be wired for that kind of work. I don't know if I have officially said anything out loud. Some seemed to have handed off SEM work to others in their research groups who are more adept.
Upon granting sign-up privileges, I advise new users whether they are free to sign up as they please or whether I would definitely like to be nearby for their next few sessions. Our reservation system, ORS, provides the option for ones to be a resource monitor by e-mail. I set that option for myself so I get notified about every change in the schedule. Most notices can be ignored. A few users are on my watch list. The delete button is easy to use for those that don't concern me.
We average about 25 hours of use per week. There is really no reason that ones HAVE to use the SEM outside of regular hours. A few users are granted or loaned keys for access at any time. Sometimes a last minute evening or weekend session is needed for a paper deadline, but that is unusual. We do allow users to come before we leave for the day and continue their session into the evening.
I do review photos occasionally from our users. Mostly I look at the images left on the SEM user interface and look deeper if I see signs of poor operation.
I plainly reference the cost of detectors during training and assure users that if they follow the standard operating procedures, they should have no problems. I also explain to them that the procedures need to be followed completely and in order. I worked hard to make the short form of the SOP short and practical. It is about 1-1/3 pages with another 2/3 page of common shortcut codes. And I tell them of some spectacular failures like ones that couldn't get an image and it was because they had failed to click the "Beam On" button on the UI.
I make it a point to refer users back to the written procedures. Otherwise I allow users to ask me too many questions and they never learn for themselves.
I hope these ideas help. I look forward to hearing what others have to say.
Warren Straszheim
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, April 10, 2015 6:09 PM To: Straszheim, Warren E [BIOTC]
X-from: roger.ristau-at-uconn.edu
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: Univ of Connecticut
Title-Subject: [Filtered] Access to User Facilities
Message: I am looking for advice from the community about how access to microscope facilities is granted to users. Specifically, I am thinking of something not quite so formal as the General User Access Proposal process available at National Labs, but not as open as our existing "free-for-all" where anyone who requests training can have open access.
I don't need advice on how to train users, rather, I need a fair process on how to determine who should or should not become a user.
Does anyone have a policy/process they wish to share--off line if you like--that weighs user requests? Perhaps a policy that includes various levels of access to users based on their needs and skills?
And a follow-up question is the magic "silver bullet" of how to monitor users after training to ensure that they are actually doing everything the way they were taught.
Thanks, Roger Ristau Univ of Connecticut
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Title-Subject: [Filtered] Access to user facilities
Message: I would say that it is important to identify those candidates which indeed have sufficient amount of work to do on SEM. Those who need 2-3 "nice pictures" to get are potentially problematic opertors with minor or zero motivation for learning and understanding the techique. The next issue is to provide the users for the approach or some signs of right way to analyse their samples. The idea is to assist users from the very beginning to find the right condition for imaging/microanalysis/diffraction pattern/etc and to explain the reasons for the choice. THe last important issue I would mention is interpersonal relations: it's better is the user (especially the new one with no experience) will feel safe and compfortable to report about any problem to the instrument/facility supervisor. Users always make mistakes, but to repair or to come back to te source of the mistake is always easier if you get the whole story and it is done ASAP. This is possible only if user does not afraid to report about a mistake. This also ensures that user will be instructed properly how to avoid this same mistake in the future. Good luck, Inna
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==============================Original Headers============================== 13, 17 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 11 09:17:25 2015 13, 17 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3BEHPGs001406 13, 17 -- for {microscopy-at-microscopy.com} ; Sat, 11 Apr 2015 09:17:25 -0500 13, 17 -- Message-ID: {55292CF5.8010705-at-microscopy.com} 13, 17 -- Date: Sat, 11 Apr 2015 09:17:25 -0500 13, 17 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 17 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 17 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 13, 17 -- MIME-Version: 1.0 13, 17 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 17 -- Subject: viaWWW:Access to user facilities 13, 17 -- References: {201504110756.t3B7usBa029672-at-ns.microscopy.com} 13, 17 -- In-Reply-To: {201504110756.t3B7usBa029672-at-ns.microscopy.com} 13, 17 -- X-Forwarded-Message-Id: {201504110756.t3B7usBa029672-at-ns.microscopy.com} 13, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I operate our SEM/TEM/confocal/epi/laser microdissection facility just like Warren does, so he saved me a lot of typing! It mostly works.
Aloha, Tina
} Please send out a summary of replies once you get some. They can remain anonymous as far as I care. } } We are probably a small enough lab that we have remained rather informal about access. } } There are a few people that I have thought about redirecting away from the microscope as their brain just doesn't seem to be wired for that kind of work. I don't know if I have officially said anything out loud. Some seemed to have handed off SEM work to others in their research groups who are more adept. } } Upon granting sign-up privileges, I advise new users whether they are free to sign up as they please or whether I would definitely like to be nearby for their next few sessions. Our reservation system, ORS, provides the option for ones to be a resource monitor by e-mail. I set that option for myself so I get notified about every change in the schedule. Most notices can be ignored. A few users are on my watch list. The delete button is easy to use for those that don't concern me. } } We average about 25 hours of use per week. There is really no reason that ones HAVE to use the SEM outside of regular hours. A few users are granted or loaned keys for access at any time. Sometimes a last minute evening or weekend session is needed for a paper deadline, but that is unusual. We do allow users to come before we leave for the day and continue their session into the evening. } } I do review photos occasionally from our users. Mostly I look at the images left on the SEM user interface and look deeper if I see signs of poor operation. } } I plainly reference the cost of detectors during training and assure users that if they follow the standard operating procedures, they should have no problems. I also explain to them that the procedures need to be followed completely and in order. I worked hard to make the short form of the SOP short and practical. It is about 1-1/3 pages with another 2/3 page of common shortcut codes. And I tell them of some spectacular failures like ones that couldn't get an image and it was because they had failed to click the "Beam On" button on the UI. } } I make it a point to refer users back to the written procedures. Otherwise I allow users to ask me too many questions and they never learn for themselves. } } I hope these ideas help. I look forward to hearing what others have to say. } } Warren Straszheim } } -----Original Message----- } X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Friday, April 10, 2015 6:09 PM } To: Straszheim, Warren E [BIOTC] } Subject: [Microscopy] viaWWW:Access to User Facilities } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: roger.ristau-at-uconn.edu } } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both roger.ristau-at-uconn.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: roger.ristau-at-uconn.edu } Name: Roger Ristau } } Organization: Univ of Connecticut } } Title-Subject: [Filtered] Access to User Facilities } } Message: I am looking for advice from the community about how access to microscope facilities is granted to users. Specifically, I am thinking of something not quite so formal as the General User Access Proposal process available at National Labs, but not as open as our existing "free-for-all" } where anyone who requests training can have open access. } } I don't need advice on how to train users, rather, I need a fair process on how to determine who should or should not become a user. } } Does anyone have a policy/process they wish to share--off line if you like--that weighs user requests? Perhaps a policy that includes various levels of access to users based on their needs and skills? } } And a follow-up question is the magic "silver bullet" of how to monitor users after training to ensure that they are actually doing everything the way they were taught. } } Thanks, } Roger Ristau } Univ of Connecticut } } Login Host: 137.99.20.243 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } -- } =========================================== } Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. 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**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 22 -- From tina-at-pbrc.hawaii.edu Sun Apr 12 14:07:21 2015 5, 22 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3CJ7Kd5002835 5, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Apr 2015 14:07:21 -0500 5, 22 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 22 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id t3CJ7Ids015508 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 22 -- Sun, 12 Apr 2015 09:07:18 -1000 (HST) 5, 22 -- Received: from localhost (tina-at-localhost) 5, 22 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id t3CJ7Hh0015505; 5, 22 -- Sun, 12 Apr 2015 09:07:17 -1000 (HST) 5, 22 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 5, 22 -- Date: Sun, 12 Apr 2015 09:07:16 -1000 (HST) 5, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 22 -- X-X-Sender: tina-at-sf-v240 5, 22 -- To: roger.ristau-at-uconn.edu, Microscopy Listserver {Microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] RE: viaWWW:Access to User Facilities 5, 22 -- In-Reply-To: {201504110202.t3B22nwY030419-at-ns.microscopy.com} 5, 22 -- Message-ID: {Pine.GSO.4.64.1504120905230.15399-at-sf-v240} 5, 22 -- References: {201504110202.t3B22nwY030419-at-ns.microscopy.com} 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
I'm in an unusual environment: a makerspace or hackerspace in Chicago, which to my knowledge has the most public access policy of any SEM.
Any adult can join for $40/month which gets them 24x7 access to the SEM, as well as access to the rest of the tools in the space.
I can't assume any science background with people who want to use it. I've put together a 3 hour PowerPoint course, which is a prerequisite. Then I schedule about 1 1/2 hours of hands on training. Then they are free to use the scope without supervision.
Generally, there is some level of self selection because there is a time commitment to go through this. Still, I find that many people don't come back and use it. This is true of many of the tools at the space. People see a scarcity of training classes on, say, the milling machine or our large CNC router, and they sign up just in case, even if they don't have a project that could use it. We haven't found a good way around this problem, and with nearly 400 members, and all tool authorizations done by volunteers, it is a source of stress for the organization.
I have put in place a tiered access structure for the SEM, and right now have only implemented the bottom tier. This training doesn't permit users to change samples, use the sputter coater, critical point dryer, backscatter detector, or EDX. Users need to work with me to prep samples, so I can make sure nobody is going to put something wet or that will outgas in the chamber. Many of the users just want to see how a SEM works, so I keep interesting samples in the chamber at all times.
So far, the only user breakage problem I've had was someone who couldn't differentiate between first and second peak, and kept raising filament current until it blew. Not that big a deal.
That's also why I've been nervous about letting anyone do sample prep, risk running the sample into the BSD, or have a liquid nitrogen accident with the EDX. Also, our sputter coater is very finicky, and the Ar pressure difference between the plasma extinguishing and the power supply overloading is quite difficult to maintain with the needle valve. Additionally I may have been too hasty buying a CPD; we don't have a fume hood, so I'm not comfortable fixing wet samples in glutaraldehyde, and we've done absolutely nothing with wet samples. (I also don't have formal training myself, and I've been hoping to find a mentor in the Chicago area to help out.)
Cheers, Ryan I'm in an unusual environment: a makerspace or hackerspace in Chicago, which to my knowledge has the most public access policy of any SEM.
Any adult can join for $40/month which gets them 24x7 access to the SEM, as well as access to the rest of the tools in the space.
I can't assume any science background with people who want to use it. I've put together a 3 hour PowerPoint course, which is a prerequisite. Then I schedule about 1 1/2 hours of hands on training. Then they are free to use the scope without supervision.
Generally, there is some level of self selection because there is a time commitment to go through this. Still, I find that many people don't come back and use it. This is true of many of the tools at the space. People see a scarcity of training classes on, say, the milling machine or our large CNC router, and they sign up just in case, even if they don't have a project that could use it. We haven't found a good way around this problem, and with nearly 400 members, and all tool authorizations done by volunteers, it is a source of stress for the organization.
I have put in place a tiered access structure for the SEM, and right now have only implemented the bottom tier. This training doesn't permit users to change samples, use the sputter coater, critical point dryer, backscatter detector, or EDX. Users need to work with me to prep samples, so I can make sure nobody is going to put something wet or that will outgas in the chamber. Many of the users just want to see how a SEM works, so I keep interesting samples in the chamber at all times.
So far, the only user breakage problem I've had was someone who couldn't differentiate between first and second peak, and kept raising filament current until it blew. Not that big a deal.
That's also why I've been nervous about letting anyone do sample prep, risk running the sample into the BSD, or have a liquid nitrogen accident with the EDX. Also, our sputter coater is very finicky, and the Ar pressure difference between the plasma extinguishing and the power supply overloading is quite difficult to maintain with the needle valve. Additionally I may have been too hasty buying a CPD; we don't have a fume hood, so I'm not comfortable fixing wet samples in glutaraldehyde, and we've done absolutely nothing with wet samples. (I also don't have formal training myself, and I've been hoping to find a mentor in the Chicago area to help out.)
Cheers, Ryan
==============================Original Headers============================== 15, 20 -- From rdpierce-at-pobox.com Sun Apr 12 14:56:21 2015 15, 20 -- Received: from elna.mackenziegems.com (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) 15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3CJuKOL023140 15, 20 -- for {Microscopy-at-Microscopy.com} ; Sun, 12 Apr 2015 14:56:20 -0500 15, 20 -- Received: from [192.168.1.107] (108-219-222-230.lightspeed.cicril.sbcglobal.net [108.219.222.230]) 15, 20 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id 85DFB6E0EDD 15, 20 -- for {Microscopy-at-Microscopy.com} ; Sun, 12 Apr 2015 14:56:20 -0500 (CDT) 15, 20 -- Subject: Re: [Microscopy] viaWWW:Access to User Facilities 15, 20 -- References: {201504121929.t3CJTgSF019516-at-ns.microscopy.com} 15, 20 -- From: Ryan Pierce {rdpierce-at-pobox.com} 15, 20 -- Content-Type: text/plain; 15, 20 -- charset=us-ascii 15, 20 -- X-Mailer: iPad Mail (12F69) 15, 20 -- In-Reply-To: {201504121929.t3CJTgSF019516-at-ns.microscopy.com} 15, 20 -- Message-Id: {00622CF2-0689-436A-8F51-EFD902F21820-at-pobox.com} 15, 20 -- Date: Sun, 12 Apr 2015 14:56:18 -0500 15, 20 -- To: Microscopy-at-Microscopy.com 15, 20 -- Mime-Version: 1.0 (1.0) 15, 20 -- Content-Transfer-Encoding: 8bit 15, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3CJuKOL023140 ==============================End of - Headers==============================
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Email: chris.guerin-at-irc.vib-ugent.be Name: Chris Guerin
Organization: VIB
Title-Subject: [Filtered] Summer school microscopy in Ghent
Message: Hello Everyone:
I am pleased to announce that the 4th VIB Summer School in Advanced Light Microscopy will take place in the beautiful city of Ghent Belgium the week of June 15th. This course will give users both practical and theoretical background on the proper use of microscopes in biological research as well as introduce them to how to use imaging to obtain functional information from cells and tissues. The uses of advanced techniques such as FRET/FLIM, FRAP, TIRF, Super-resolution, and correlative light and electron microscopy will be explained and demonstrated. Hands on practical sessions will allow students to use and observe advanced microscope systems and to understand the best ways to design and carry out modern bioimaging. The final day will be dedicated to data analysis and interpretation of microscope generated data sets. This year the faculty of expert microscopists will include: Chris Guerin, VIB - Ghent University, Belgium Sebastian Munck, VIB - KU Leuven, Belgium Peter O’Toole, University of York, UK Bob Asselbergh, University of Antwerp, Belgium Stefan Vinckier, VIB - KU Leuven, Belgium Spencer Shorte, Institut Pasteur, France Lucy Collinson, London Research Institute, UK Andreas Schertel, Carl Zeiss, Germany Jean-Yves Tinevez, Institut Pasteur, France Eef Parthoens, VIB - Ghent University, Belgium You can see the full program and register at http://www.vib.be/en/training/research-training/courses/Pages/Summer-School-in-Advanced-Light-Microscopy.aspx. The course is limited to 32 participants and a simple application is required in case, (as in previous years), that the number of interested people exceeds our capacity. Registration is €450 for the week long course and includes a daily lunch and dinner Mon, Tues and Thurs. evenings. We look forward to welcoming you to Ghent.
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 13 16:32:42 2015 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3DLWgna009165 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:32:42 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 7F06541616D 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:32:42 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at znl.com 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id EgcaxHBHO02R for {microscopy-at-microscopy.com} ; 13, 28 -- Mon, 13 Apr 2015 16:32:33 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 2B8B0416165 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:32:33 -0500 (CDT) 13, 28 -- Message-ID: {552C35F0.6000303-at-microscopy.com} 13, 28 -- Date: Mon, 13 Apr 2015 16:32:32 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:4th VIB Summer School in Advanced Light Microscopy in Ghent 13, 28 -- References: {201504131220.t3DCKcLX031307-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201504131220.t3DCKcLX031307-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201504131220.t3DCKcLX031307-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 13, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Kansas State University
Title-Subject: [Microscopy] viaWWW:Access to User Facilities
Message: Hi Roger, I have 6 years of experience in EM lab management. I was giving training to 5 user for TEM operation per semester. First step, I will define their real need of EM based on research activity and average usage of their research group.
1. I used to call user for 6 hours (2 hours per consecutive 3 days) for observation only. 2. One week step wise training (focusing, imaging, sample change, how to switch on-off machine). 3. Written test (10-15 MCQ on TEM operation) one assignment on SOP to operate TEM. (I never use to give them SOP, they have to make themselves and it has to approved by lab manager before use.) 4. Practical test on TEM by lab manager.
Permission to use TEM withdrawn, If any qualified user make three mistake (left the machine without following proper closing procedure, drastic change in alignment) and fail to report any incident or error.
Ravi.
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 13 16:33:35 2015 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3DLXZKJ009722 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:33:35 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id B7055416179 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:33:35 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at znl.com 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id c8FUrl-DkAX9 for {microscopy-at-microscopy.com} ; 16, 28 -- Mon, 13 Apr 2015 16:33:24 -0500 (CDT) 16, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id CAB7D416171 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2015 16:33:24 -0500 (CDT) 16, 28 -- Message-ID: {552C3624.2030403-at-microscopy.com} 16, 28 -- Date: Mon, 13 Apr 2015 16:33:24 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:Access to User Facilities 16, 28 -- References: {201504132047.t3DKlX0e008286-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201504132047.t3DKlX0e008286-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201504132047.t3DKlX0e008286-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Before you get to excited we are based in the UK in Bath and we would expect anyone who wants these items to collect them at their expense. I will give priority to people who want whole items but should any of you want parts let me know and if no takers for the whole thing then they are yours.
We have a Gatan Duo Ion Mill 600 diff pumped with sector and cryo cooler. It works but is getting tired so I will say 'spares or repair' It comes with a large assortment of consumables. If anyone want the whole thing then fine otherwise I am willing to offer the parts on first come first serve basis.
We also have a vintage dimple grinder and core drill. If these are any good to any of you then you are welcome to it but when I say vintage I mean 1981! it still works.
We also have some struers tenupol 2 systems again vintage but functional.
I look forward to hearing from you if you can save these from the scrap pile.
John
==============================Original Headers============================== 7, 26 -- From john.mitchels-at-gmail.com Tue Apr 14 13:28:44 2015 7, 26 -- Received: from mail-ig0-f176.google.com (mail-ig0-f176.google.com [209.85.213.176]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3EISi6h015033 7, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 13:28:44 -0500 7, 26 -- Received: by iget9 with SMTP id t9so80932978ige.1 7, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 11:28:43 -0700 (PDT) 7, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 26 -- d=gmail.com; s=20120113; 7, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 7, 26 -- bh=MQbIuyYuUj8Rc6+3sNJ921plKuY1BJ9svAIIJO76/lU=; 7, 26 -- b=HlJQYa1iaOdKLrw4JLYI4Vi9EzflN9eVKubCg/HWO6lE/JEKFI/jEDRCEF8EMUsLqs 7, 26 -- tyan9TgQff/YHJdNfe07saxLD0vGx9gYqYp5Kapwx9yyZwK7VsOalB0msEUyNXGbUfTR 7, 26 -- oSPb/e1PifHuiwbxBV/TgBVLtnshDPTsvd72rXJd2Bb+073iEmaTlQUG/L9u0Bo0Dn6V 7, 26 -- r9t3iD6G2flNj9GK9098Yqj0d/JAb6l/BrLbSzh1PF/jNkSKYOShA313j77wQb+/vJz5 7, 26 -- ThK4P5RxolSFdpmmKbZqx9519pwsiqtxP7xIkuL5DAbCIQUXEDIUcpNNb8+LH/UZSaZI 7, 26 -- 7RRg== 7, 26 -- MIME-Version: 1.0 7, 26 -- X-Received: by 10.50.43.162 with SMTP id x2mr26117609igl.46.1429036123732; 7, 26 -- Tue, 14 Apr 2015 11:28:43 -0700 (PDT) 7, 26 -- Received: by 10.36.17.19 with HTTP; Tue, 14 Apr 2015 11:28:43 -0700 (PDT) 7, 26 -- Date: Tue, 14 Apr 2015 19:28:43 +0100 7, 26 -- Message-ID: {CAHX7yTS=z+nw6vPOR_1WmC82HAP_7cwzthzeFXO9V7K7mqRdUw-at-mail.gmail.com} 7, 26 -- Subject: Free to good home Ion Mill, Dimple Grinder and Electro jet polishers (UK) 7, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
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Email: roy.geiss-at-colostate.edu Name: ROy Geiss
Organization: Colorado State University
Title-Subject: [Filtered] Summer School on Electron Diffraction
Message: Just a reminder that Wednesday, April 15 is the last day to register for the upcoming Summer School on Electron Diffraction at Colorado State University from May 19 to May 21, 2015. For a flyer, the program, and registration materials please go to: http://cif.colostate.edu/ and follow the links to the Summer school.
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 14 20:14:12 2015 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3F1ECt9012287 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 20:14:12 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 9402A4178AB 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 20:14:12 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at znl.com 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id CyLo1eoDc11w for {microscopy-at-microscopy.com} ; 14, 28 -- Tue, 14 Apr 2015 20:14:05 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (unknown [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 5776F4178A2 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2015 20:14:05 -0500 (CDT) 14, 28 -- Message-ID: {552DBB5C.2060407-at-microscopy.com} 14, 28 -- Date: Tue, 14 Apr 2015 20:14:04 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Summer School on Electron Diffraction 14, 28 -- References: {201504142339.t3ENd5AE010203-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201504142339.t3ENd5AE010203-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201504142339.t3ENd5AE010203-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: Are there any Canadian listers out there who are using an Edwards 1105 vacuum controller. I've bought one with gauges off ebay, but it doesn't have UL or CSA inspection stickers so the university won't approve me using it. If anyone has one in use a photo of the back with the stickers would go a long way towards getting it approved.
Thanks in advance for any help
Glenn
I do subscribe to the list but my institution insists on attaching advertising to the bottom of all my email, thus ensuring rejection by the very effective spam filters
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Apr 16 08:27:14 2015 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3GDRDgL004754 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 08:27:13 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 1E62C419687 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 08:27:13 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at znl.com 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id TvVtrwGxI2us for {microscopy-at-microscopy.com} ; 13, 28 -- Thu, 16 Apr 2015 08:27:05 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id BFB5941967B 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 08:27:05 -0500 (CDT) 13, 28 -- Message-ID: {552FB8A9.2030207-at-microscopy.com} 13, 28 -- Date: Thu, 16 Apr 2015 08:27:05 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Edwards 1105 vacuum controller 13, 28 -- References: {201504161322.t3GDMfUi004706-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201504161322.t3GDMfUi004706-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201504161322.t3GDMfUi004706-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We would like to bring your attention to the workshop "Big, Deep, and Smart Data Analytics in Materials Imaging" organized jointly by the five DOE Nanoscale Science Research Centers (Program Committee: E. Stach, J. Werner, D. Miller, S.V. Kalinin and J. Schuck) and to be held at Oak Ridge National Laboratory on June 8-10. This workshop will bring together researchers from different imaging disciplines (electron microscopy, scanning probe microscopy, focused x-ray, neutron, atom probe tomography, chemical imaging, optical microscopies) as well as experts in mathematical/statistical/computational approaches to discuss opportunities and future needs in the integration of advanced data analytics and theory into imaging science. It will provide a forum to present achievements in the various imaging disciplines with emphasis on acquisition, visualization, and analysis of multidimensional data sets, the corresponding approaches for theory-experiment matching, and novel opportunities for instrumental development enabled by the availability of high speed data analytic tools. Additional information regarding this workshop will be posted at the http://www.cnms.ornl.gov/JointNSRC2015/.
Please address questions to Amanda Zetans, zetansac-at-ornl.gov {mailto:zetansac-at-ornl.gov} , 865.241.1182.
On behalf of the organizers,
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Theme Leader, Center for Nanophase Materials Science
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 10, 23 -- From sergei2-at-ornl.gov Thu Apr 16 16:21:37 2015 10, 23 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3GLLbv9008227 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 16:21:37 -0500 10, 23 -- X-SG: RELAYLIST 10, 23 -- X-IronPort-AV: E=Sophos;i="5.11,590,1422939600"; 10, 23 -- d="scan'208";a="78488049" 10, 23 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 10, 23 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 16 Apr 2015 17:21:37 -0400 10, 23 -- Received: from [128.219.192.92] (pc83680.ornl.gov [128.219.192.92]) 10, 23 -- (using TLSv1 with cipher DHE-RSA-CAMELLIA256-SHA (256/256 bits)) 10, 23 -- (No client certificate requested) 10, 23 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 275D3E01F4 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Apr 2015 17:21:37 -0400 (EDT) 10, 23 -- Message-ID: {553027E0.8040803-at-ornl.gov} 10, 23 -- Date: Thu, 16 Apr 2015 17:21:37 -0400 10, 23 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 10, 23 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:14.0) Gecko/20120713 Thunderbird/14.0 10, 23 -- MIME-Version: 1.0 10, 23 -- To: microscopy-at-microscopy.com 10, 23 -- Subject: Workshop - Big, Deep, and Smart Data Analytics in Materials Imaging 10, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: susan.vanhorn-at-stonybrook.edu Name: Susan C Van Horn
Organization: Central Microscopy Imaging Center
Title-Subject: [Filtered] embedding yeast
Message: I always have problems embedding/infiltrating yeast!!! have tried different resins, vacuum steps,etc.. does anyone have an embedding protocol/resin that works??!!! thanks for sharing sue
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Apr 17 07:29:33 2015 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3HCTXEL027358 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:33 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 440F341AA1F 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:33 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at znl.com 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id M6SZ3DeJ6q02 for {microscopy-at-microscopy.com} ; 11, 28 -- Fri, 17 Apr 2015 07:29:21 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 64DA441AA14 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:21 -0500 (CDT) 11, 28 -- Message-ID: {5530FCA1.8000603-at-microscopy.com} 11, 28 -- Date: Fri, 17 Apr 2015 07:29:21 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:embedding yeast 11, 28 -- References: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: Pruessner-at-ukzn.ac.za Name: Karin Pruessner
Organization: University of KwaZulu-Natal, Durban
Title-Subject: [Filtered] Postdoctoral poasition in energy materials
Message: The University of KwaZulu-Natal in Durban, South Africa, is home to an interdisciplinary Nanotechnology Platform consisting of 4 pillars in Nano Energy, Nano Materials, Nano Health and Quantum Physics. The Nano Energy pillar has at least one, possibly two, positions available for a Postdoctoral Researcher to be filled as soon as possible. Our team consists of Chemists, Physicists, Materials Scientists, and Engineers. We are working on materials for energy applications, specifically on the development of an off-grid refrigeration unit for rural areas in Africa. We are looking for a Materials Scientist with a solid background in Materials Characterization to join us. Neighboring fields will be considered. Hands-on experience in Scanning and Transmission Electron Microscopy, X-Ray Diffraction, and Raman Spectroscopy is expected. Experience in at least one of the following areas would be beneficial: • Nano Photovoltaics • Nano Energy Storage • Nano Cooling Fluids • Graphene The university houses an Electron Microscopy Unit. XRD and Raman Spectroscopy are available in the School of Chemistry and Physics. Advanced instrumentation including aberration-corrected HRTEM is available through national user centers. Interested candidates should send their electronic application materials to: Dr Karin Pruessner UKZN Nanotechnology Platform, Nano Energy Coordinator School of Chemistry and Physics University of KwaZulu-Natal - Westville Campus University Road Durban, 4000 South Africa Ph: ++27 31 260-7660 e-mail: Pruessner-at-ukzn.ac.za
Review of applications will begin immediately and continue until the posts are filled.
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Not sure what type of yeast you work with. We work with budding yeast and have been had good luck with microwave radiation, especially for stationary yeast, of which the cell wall is very tough. http://www.ncbi.nlm.nih.gov/pubmed/17156022
For log-phase cells, extended infiltration (at least one overnight) works fine using Spurr. I believe the low viscosity of Spurr helps.
Best luck and let me know if you need a reprint.
Zhaojie Zhang University of Wyoming
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, April 17, 2015 6:39 AM To: Z.J. Zhang
X-from: susan.vanhorn-at-stonybrook.edu
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Email: susan.vanhorn-at-stonybrook.edu Name: Susan C Van Horn
Organization: Central Microscopy Imaging Center
Title-Subject: [Filtered] embedding yeast
Message: I always have problems embedding/infiltrating yeast!!! have tried different resins, vacuum steps,etc.. does anyone have an embedding protocol/resin that works??!!! thanks for sharing sue
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Apr 17 07:29:33 2015 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3HCTXEL027358 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:33 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 440F341AA1F 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:33 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at znl.com 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (znl.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id M6SZ3DeJ6q02 for {microscopy-at-microscopy.com} ; 11, 28 -- Fri, 17 Apr 2015 07:29:21 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro-ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 64DA441AA14 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2015 07:29:21 -0500 (CDT) 11, 28 -- Message-ID: {5530FCA1.8000603-at-microscopy.com} 11, 28 -- Date: Fri, 17 Apr 2015 07:29:21 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:31.0) Gecko/20100101 Thunderbird/31.6.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:embedding yeast 11, 28 -- References: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201504161353.t3GDrbQ5024576-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
dear Sue, there are many resources in the web concerning the (tricky) task to properly embed yeast cells for ultrathin sectioning. my favourite ones, as of today: Mary's manual (Boulder, CO, USA): http://bio3d.colorado.edu/docs/mmanual.pdf then Giddings, T. H., Jr., O’Toole, E. T., Morphew, M., Mastronarde, D. N., McIntosh, J. R., and Winey, M. (2001). Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis. Methods Cell Biol. 67, 27–42 (yes, Mary is one of the co-authors), and Kent L McDonald - Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material, -- Protoplasma (2014) 251:429–448 DOI 10.1007/s00709-013-0575-y. This article is quite helpful as it shows that some of the paradigms of 'old' embedding protocols are clearly out-dated, if not to say "wrong". It also depends on your equipment, the specific question, and so on. kind regards - reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: http://www.mc2015.de DGE - Microscopy Conference 2015, Sept 6-11, Göttingen http://www.emc2016.fr/en/ 16th Europ Microsc Congress EMC 2016 in Lyon, FR next Microbiol. conferences: VAAM - Annual Conf March 13-16, 2016, Jena
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Hi folks, I am acquiring a Jeol JSM-T200 SEM and wanted to try and dig up some info on it, or similar models. Mainly interested in the raster scanning circuitry, as this seems to need some work or possibly be re-implemented from scratch.
Any info would be helpful, at this point I've acquired the user-manual... so I guess I'm looking for the service-manual now.
I'll even take info on similar models, since that is likely better than having no clues at all!
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Email: martin.roe-at-nottingham.ac.uk Name: Martin Roe
Organization: Nottingham University
Title-Subject: [Filtered] JEOL 6400 SEM Diffusion pump heater element
Message: Dear Listservers, Does anyone have a spare diffusion pump heater element for a JEOL 6400 SEM? There are two diff pumps on the instrument and it is the larger of the two that has the heater problem. The original part number of the heater is 322000025 and is 4 inches in diameter (rated at 200V/600W) with what seemed like “TK” written on it. Would be willing to pay for the part and shipment of it. Thanks, Martin
Martin Roe Department of Mechanical, Materials & Manufacturing Faculty of Engineering University of Nottingham, Wolfson Building, University Park, Nottingham, NG7 2RD, UK
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NIST DTSA-II has recently been updated to version Iona. (Download for free from http://www.cstl.nist.gov/div837/837.02/epq/dtsa2/index.html) DTSA-II provides a host of tools for quantitative EDS microanalysis including quantification, simulation and measurement planning. Iona has a host of improvements both large and small which are detailed in the release notes on the web site.
Further details on quantitative analysis with NIST DTSA-II are available in Newbury & Ritchie's J. Mat Sc. Article "Performing elemental microanalysis with high accuracy and high precision by scanning electron microscopy/silicon drift detector energy-dispersive X-ray spectrometry (SEM/SDD-EDS)" (free for download from http://link.springer.com/article/10.1007/s10853-014-8685-2 ). This article demonstrates the potential of the modern EDS detector to perform reliable, quantitatively accurate compositional measurements even for some very challenging samples.
==============================Original Headers============================== 5, 42 -- From nicholas.ritchie-at-nist.gov Tue Apr 21 12:40:33 2015 5, 42 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1bon0117.outbound.protection.outlook.com [157.56.111.117]) 5, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3LHeXfe002896 5, 42 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2015 12:40:33 -0500 5, 42 -- Received: from BN3PR09MB0595.namprd09.prod.outlook.com (25.160.119.28) by 5, 42 -- BN3PR09MB0595.namprd09.prod.outlook.com (25.160.119.28) with Microsoft SMTP 5, 42 -- Server (TLS) id 15.1.136.25; Tue, 21 Apr 2015 17:40:32 +0000 5, 42 -- Received: from BN3PR09MB0595.namprd09.prod.outlook.com ([25.160.119.28]) by 5, 42 -- BN3PR09MB0595.namprd09.prod.outlook.com ([25.160.119.28]) with mapi id 5, 42 -- 15.01.0136.026; Tue, 21 Apr 2015 17:40:32 +0000 5, 42 -- From: "Ritchie, Nicholas" {nicholas.ritchie-at-nist.gov} 5, 42 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 42 -- Subject: NIST DTSA-II Iona released & high precision quant with an SDD 5, 42 -- Thread-Topic: NIST DTSA-II Iona released & high precision quant with an SDD 5, 42 -- Thread-Index: AdB8WH/3zCY7ftpPRVWY5nHI2M73bw== 5, 42 -- Date: Tue, 21 Apr 2015 17:40:31 +0000 5, 42 -- Message-ID: {BN3PR09MB05958089BF34F4467D081439F1EF0-at-BN3PR09MB0595.namprd09.prod.outlook.com} 5, 42 -- Accept-Language: en-US 5, 42 -- Content-Language: en-US 5, 42 -- X-MS-Has-Attach: 5, 42 -- X-MS-TNEF-Correlator: 5, 42 -- authentication-results: spf=none (sender IP is ) 5, 42 -- smtp.mailfrom=nicholas.ritchie-at-nist.gov; 5, 42 -- x-originating-ip: [129.6.126.223] 5, 42 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BN3PR09MB0595; 5, 42 -- x-forefront-antispam-report: BMV:1;SFV:NSPM;SFS:(10019020)(979002)(6009001)(11905935001)(77156002)(40100003)(2656002)(50986999)(15188555004)(54356999)(15975445007)(102836002)(74316001)(46102003)(86362001)(87936001)(122556002)(19580395003)(2501003)(450100001)(107886001)(92566002)(229853001)(2900100001)(76576001)(66066001)(99286002)(33656002)(110136001)(2351001)(62966003)(969003)(989001)(999001)(1009001)(1019001);DIR:OUT;SFP:1102;SCL:1;SRVR:BN3PR09MB0595;H:BN3PR09MB0595.namprd09.prod.outlook.com;FPR:;SPF:None;MLV:ovrnspm;PTR:InfoNoRecords;LANG:en; 5, 42 -- x-microsoft-antispam-prvs: {BN3PR09MB059526675199707646AF126BF1EF0-at-BN3PR09MB0595.namprd09.prod.outlook.com} 5, 42 -- x-exchange-antispam-report-test: UriScan:; 5, 42 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5002010)(5005006);SRVR:BN3PR09MB0595;BCL:0;PCL:0;RULEID:;SRVR:BN3PR09MB0595; 5, 42 -- x-forefront-prvs: 0553CBB77A 5, 42 -- received-spf: None (protection.outlook.com: nist.gov does not designate 5, 42 -- permitted sender hosts) 5, 42 -- Content-Type: text/plain; charset="us-ascii" 5, 42 -- MIME-Version: 1.0 5, 42 -- X-OriginatorOrg: nist.gov 5, 42 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 21 Apr 2015 17:40:31.7353 5, 42 -- (UTC) 5, 42 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 5, 42 -- X-MS-Exchange-CrossTenant-id: 2ab5d82f-d8fa-4797-a93e-054655c61dec 5, 42 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN3PR09MB0595 5, 42 -- Content-Transfer-Encoding: 8bit 5, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3LHeXfe002896 ==============================End of - Headers==============================
A colleague has an old JEOL JAM-7830 Auger microprobe. He has had troubles starting it back up after the last couple power outages. The system fails to recognize the field emission gun and instead thinks it has a LaB6 source.
He has been able to stumble around and eventually get it to work by trying various things, but the results are not immediately obvious and he is not clear which steps have been effective and necessary or if the order of steps is important.
Does anyone have experience with this problem on a 7830 and have some suggestions for him? We thank you in advance.
Warren Straszheim Ames Lab/Iowa State University
==============================Original Headers============================== 5, 40 -- From wesaia-at-iastate.edu Tue Apr 21 18:03:17 2015 5, 40 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2on0095.outbound.protection.outlook.com [207.46.100.95]) 5, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3LN3G9p011221 5, 40 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2015 18:03:16 -0500 5, 40 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 5, 40 -- BLUPR04MB548.namprd04.prod.outlook.com (10.141.29.152) with Microsoft SMTP 5, 40 -- Server (TLS) id 15.1.136.25; Tue, 21 Apr 2015 23:03:15 +0000 5, 40 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.116]) by 5, 40 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.116]) with mapi id 5, 40 -- 15.01.0136.014; Tue, 21 Apr 2015 23:03:15 +0000 5, 40 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 5, 40 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 40 -- Subject: Problem with Auger gun startup 5, 40 -- Thread-Topic: Problem with Auger gun startup 5, 40 -- Thread-Index: AdB8hoYj/rAzWfGiRNCIvyhA2Vmi7Q== 5, 40 -- Date: Tue, 21 Apr 2015 23:03:15 +0000 5, 40 -- Message-ID: {BLUPR04MB546DE08E1D8AC17743D80CFD7EF0-at-BLUPR04MB546.namprd04.prod.outlook.com} 5, 40 -- Accept-Language: en-US 5, 40 -- Content-Language: en-US 5, 40 -- X-MS-Has-Attach: 5, 40 -- X-MS-TNEF-Correlator: 5, 40 -- authentication-results: microscopy.com; dkim=none (message not signed) 5, 40 -- header.d=none; 5, 40 -- x-originating-ip: [129.186.227.4] 5, 40 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BLUPR04MB548; 5, 40 -- x-microsoft-antispam-prvs: {BLUPR04MB548B39B6B4CA182CF79CA7BD7EF0-at-BLUPR04MB548.namprd04.prod.outlook.com} 5, 40 -- x-forefront-antispam-report: BMV:1;SFV:NSPM;SFS:(10009020)(6009001)(504964003)(99286002)(40100003)(2351001)(76576001)(107886001)(86362001)(90282001)(2900100001)(450100001)(110136001)(2501003)(33656002)(92566002)(75432002)(74316001)(54356999)(50986999)(2656002)(87936001)(122556002)(62966003)(88552001)(229853001)(89122001)(46102003)(66066001)(102836002)(77156002);DIR:OUT;SFP:1101;SCL:1;SRVR:BLUPR04MB548;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 5, 40 -- x-exchange-antispam-report-test: UriScan:; 5, 40 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(5005006)(5002010);SRVR:BLUPR04MB548;BCL:0;PCL:0;RULEID:;SRVR:BLUPR04MB548; 5, 40 -- x-forefront-prvs: 0553CBB77A 5, 40 -- Content-Type: text/plain; charset="us-ascii" 5, 40 -- MIME-Version: 1.0 5, 40 -- X-OriginatorOrg: iastate.edu 5, 40 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 21 Apr 2015 23:03:15.6208 5, 40 -- (UTC) 5, 40 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 5, 40 -- X-MS-Exchange-CrossTenant-id: 0347d89a-0174-4dd3-adeb-3339c89c35f5 5, 40 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BLUPR04MB548 5, 40 -- Content-Transfer-Encoding: 8bit 5, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3LN3G9p011221 ==============================End of - Headers==============================
I find myself in a little bit of a quandary. ARDL would like to upgrade our low to medium light imaging capacity. One of the quandaries is the fellow with the purse strings has demands. He wants a simple imaging system with the highest resolution at a reasonable price.
I'm looking for a stereomicroscope with a good, flat field, corrected lens for color and aberration. A course and fine focus would be well received as would a translumination base, but these are not deal breakers. I'd like a camera with about 5-12 megapixals. I'd like software with some basic annotating features, image processing ability and measurement functions. The software should have file storage system suitable for both scientific and legal applications.
If you're a vendor, if you have experience with these systems, if you have an axe to grind or just want to get in on the conversation please get in touch with me. I'd like to get demos if possible.
It's been suggest we are in a rush to spend money, but......I understand the reputation we have with the closed purse. I don't think I have to say anything else.
Thank you!
Frank Karl ARDL Frank_karl-at-ARDL.com 330-794-6600
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 10, 28 -- From frank_karl-at-ardl.com Thu Apr 23 13:02:41 2015 10, 28 -- Received: from cal1-mh775.smtproutes.com (cal1-mh775.smtproutes.com [208.70.91.141]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3NI2ePp031802 10, 28 -- for {microscopy-at-microscopy.com} ; Thu, 23 Apr 2015 13:02:41 -0500 10, 28 -- X-Katharion-ID: 1429812143.35221.cal1-mh775 10, 28 -- Received: from exchange2k7.ad.ardl.com ([98.100.51.26]) by 10, 28 -- cal1-mh775.smtproutes.com [(192.69.16.141)] with ESMTP via TCP 10, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 23 Apr 2015 18:02:23 +0000 10, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 10, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Thu, 23 10, 28 -- Apr 2015 14:02:04 -0400 10, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 10, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 10, 28 -- {microscopy-at-microscopy.com} 10, 28 -- Date: Thu, 23 Apr 2015 14:02:03 -0400 10, 28 -- Subject: Stereomicroscope and cameria 10, 28 -- Thread-Topic: Stereomicroscope and cameria 10, 28 -- Thread-Index: AdB975qsNmEk30WkQ4eftJNgFUeMbQ== 10, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772CA5D9EB69-at-exchange2k7.ad.ardl.com} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id t3NI2ePp031802 ==============================End of - Headers==============================
Re: Microscope - I'm sure that a number of vendors will contact you Re: Camera + SW - have forwarded your request to a colleague who specializes in that area and has some clever solution
One thing to consider which is economical and also dramatically expands your stereomicroscopy is the fluorescence system from Nightsea (Nightsea.com ... Caveat: No financial interest). It has been interesting to see the response at a variety of meetings I've attended over the last 6 months. Although fluorescence came up through the ranks via the Life Sciences, there are intriguing applications in materials sciences as well. I remember looking at pockets/bubbles in an elastomer to determine if they had air or gel. As I remember, the gel fluoresced. Also great when used with fluorescent dyes for imaging surface cracks/defects. Nightsea's inventor, Dr. Charlie Mazel, is always interested in looking at new things. I recommend giving him a call and sending him some samples to see if this should be part of your planned upgrade.
Hope this was helpful.
Good hunting! Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education ... "Education, not Training" 7101 Royal Glen Trail, Suite A - McKinney, TX 75070 - P: 972-924-5310 www.MicroscopyEducation.com
NEW! Getting involved in Raman or FTIR? MME is now offering courses in these areas specifically for microscopists! Now scheduling courses through the end of 2015. We can customize a course on nearly any topic, from fluorescence to confocal to image analysis to SEM/TEM. Call today for a free training evaluation.
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==============================Original Headers============================== 23, 29 -- From bfoster-at-the-mip.com Thu Apr 23 17:42:58 2015 23, 29 -- Received: from barcelona.directrouter.com (barcelona.directrouter.com [72.249.98.64]) 23, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3NMgvBL004553 23, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 23 Apr 2015 17:42:57 -0500 23, 29 -- Message-Id: {201504232242.t3NMgvBL004553-at-ns.microscopy.com} 23, 29 -- Received: from 99-168-104-53.lightspeed.rcsntx.sbcglobal.net ([99.168.104.53]:60683 helo=Bs-PC.the-mip.com) 23, 29 -- by barcelona.directrouter.com with esmtpsa (TLSv1:DHE-RSA-AES256-SHA:256) 23, 29 -- (Exim 4.85) 23, 29 -- (envelope-from {bfoster-at-the-mip.com} ) 23, 29 -- id 1YlPpc-0007kB-DA; Thu, 23 Apr 2015 17:42:52 -0500 23, 29 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 23, 29 -- Date: Thu, 23 Apr 2015 17:42:48 -0500 23, 29 -- To: frank_karl-at-ardl.com, 23, 29 -- "MIcroscopy-Microscopy.com" {Microscopy-at-Microscopy.com} 23, 29 -- From: Barbara Foster {bfoster-at-the-mip.com} 23, 29 -- Subject: Re: [Microscopy] Stereomicroscope and cameria 23, 29 -- In-Reply-To: {201504231816.t3NIGN6w012025-at-ns.microscopy.com} 23, 29 -- References: {201504231816.t3NIGN6w012025-at-ns.microscopy.com} 23, 29 -- Mime-Version: 1.0 23, 29 -- Content-Type: text/plain; charset="us-ascii" 23, 29 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 23, 29 -- X-AntiAbuse: Primary Hostname - barcelona.directrouter.com 23, 29 -- X-AntiAbuse: Original Domain - microscopy.com 23, 29 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 23, 29 -- X-AntiAbuse: Sender Address Domain - the-mip.com 23, 29 -- X-Get-Message-Sender-Via: barcelona.directrouter.com: authenticated_id: bfoster-at-the-mip.com 23, 29 -- X-Source: 23, 29 -- X-Source-Args: 23, 29 -- X-Source-Dir: ==============================End of - Headers==============================
Does anyone have a spare diode holder (preferably with diode) for the GW 113A backscatter detector?
This is for an S-800 that I have been restoring for the past year for personal / educational use. I have the GW arm, the 113A electronics, and the external beam monitor electronics (for compensate for the cold field emission tip).
Any help in sourcing the diode holder / diode would be appreciated. Even drawings of this diode holder would be helpful since I could always make one up!
Hello, We are seeking a Microscopy Facility Specialist here at the Johns Hopkins School of Medicine Microscope Facility, Baltimore MD. The main focus is on fluorescence and confocal microscopy operation, training and maintenance, with emphasis on computer based analysis. All interested parties should apply at: https://hrnt.jhu.edu/jhujobs/job_view.cfm?view_req_id=64229&view=sch
We look forward to reading your resumes.
Sincerely, Michael Delannoy Assoc. Director JHU SOM Microscope Facility
The University of Bath's Microscopy and Analysis Facility (in the UK) is looking for a microscopy specialist to look after users from chemistry, physics and engineering. The job is advertised at the following link. Note the deadline is the 10th May with interviews on the 18th ideally. The role will be to look after the suite collection of electron microscopes. Candidates with experience of maintenance of older equipment, elemental analysis, diffraction analysis and if possible Raman, FTIR, and SPM knowledge.
This link is for the job: http://www.bath.ac.uk/jobs/Vacancy.aspx?ref=SS3127
This link is for the suite: http://www.bath.ac.uk/facilities/mas
==============================Original Headers============================== 4, 26 -- From john.mitchels-at-gmail.com Mon Apr 27 11:19:33 2015 4, 26 -- Received: from mail-ie0-f175.google.com (mail-ie0-f175.google.com [209.85.223.175]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id t3RGJXHM028015 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 11:19:33 -0500 4, 26 -- Received: by iedfl3 with SMTP id fl3so147192867ied.1 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2015 09:19:32 -0700 (PDT) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=20120113; 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 26 -- bh=/YKIbA1sQJo4uBt8nJfMT2OyAlGoxSm7DdSiFNViElc=; 4, 26 -- b=PRvhpnXBEpZIOwBAtY2stimWb+N/surS6OICZ5GabW5OxL2rNXegnh82IDCIxDfvb0 4, 26 -- nEZFzw7WIOGCpTG1OlmQ/+9S4ePYDYb2JYBJrS7Q+exWNflREAomF7xnimBAkaGPKW8K 4, 26 -- 1TJ2M3v/NVol8i0rNwPjCqkJLfKxEtW4s0SqAtcleK7SJM4lGtzI5SP/6E8QR1J8OgqF 4, 26 -- EFzP25uqubnQyuSt9S60tm1hVOdSg0L6fGIYZS/7FJJPf2+5EGmBEZZSAmtJY5UHb9dD 4, 26 -- h/6/wttRsKkxH+xsnLfbm9IxnceMfMrwE8ApQEb0eW43ZucMIl+gWFwKnA9+hHHbqx83 4, 26 -- 5GYw== 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Received: by 10.107.157.70 with SMTP id g67mr15199886ioe.11.1430151572547; 4, 26 -- Mon, 27 Apr 2015 09:19:32 -0700 (PDT) 4, 26 -- Received: by 10.36.17.19 with HTTP; Mon, 27 Apr 2015 09:19:32 -0700 (PDT) 4, 26 -- Date: Mon, 27 Apr 2015 17:19:32 +0100 4, 26 -- Message-ID: {CAHX7yTTTwr4qRPGUDLeuPnTv3aZ75y_9t5thqDfDvJMrK8XOqw-at-mail.gmail.com} 4, 26 -- Subject: Materials Microscopy Specialist Position Bath, UK 4, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
I would like to revisit the problem of old software, computers, and institutional support.
We have many instruments that run on proprietary software that has not been upgraded to more modern operating systems.
For example, some of our instruments use programs only compatible with Windows XP.
Our IT guys want to 'upgrade' all campus computers to a newer operating system and don't want any old machines running.
Is it reasonable to tell them that we need our old XP (and earlier) computers to keep our instruments running.
What would be a good approach to satisfy their urge to stay current and our need to live in the past?
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
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