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==============================Original Headers============================== 20, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 1 08:41:49 2014 20, 25 -- Received: from znl.com ([206.69.208.20]) 20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s01EfnJu013746 20, 25 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jan 2014 08:41:49 -0600 20, 25 -- Received: from localhost (localhost [127.0.0.1]) 20, 25 -- by znl.com (Postfix) with ESMTP id 7728769F1F8 20, 25 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jan 2014 08:41:49 -0600 (CST) 20, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 20, 25 -- Received: from znl.com ([127.0.0.1]) 20, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 20, 25 -- with ESMTP id 2aSIXMr187vE for {microscopy-at-microscopy.com} ; 20, 25 -- Wed, 1 Jan 2014 08:41:44 -0600 (CST) 20, 25 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 20, 25 -- by znl.com (Postfix) with ESMTPA id 1F85F69F1E8 20, 25 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jan 2014 08:41:44 -0600 (CST) 20, 25 -- Message-ID: {52C42927.8020603-at-microscopy.com} 20, 25 -- Date: Wed, 01 Jan 2014 08:41:43 -0600 20, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 25 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 25 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 20, 25 -- MIME-Version: 1.0 20, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 25 -- Subject: Administrivia: Happy New Year 2014 20, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 20, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Could anyone give me an advice about the FE-SEM JSM-7100FT? Is it a good equipment for working with metal alloys, steels, considering I must have good images in a variety of microstructures, good and fast texture analysis (EBSD) and EDX? Can it be placed on the same level as the ZEISS Sigma HD?
I don't know any institution where there is such a microscope - FE-SEM JSM-7100FT - in function. Do you?
Thanks in advance
Margareth
Technology Center Institute of Metalurgy and Special Alloys Brazil
==============================Original Headers============================== 7, 46 -- From margareth.andrade-at-fiemg.com.br Thu Jan 2 06:58:32 2014 7, 46 -- Received: from co9outboundpool.messaging.microsoft.com (co9ehsobe003.messaging.microsoft.com [207.46.163.26]) 7, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s02CwVCl015853 7, 46 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 06:58:31 -0600 7, 46 -- Received: from mail160-co9-R.bigfish.com (10.236.132.229) by 7, 46 -- CO9EHSOBE032.bigfish.com (10.236.130.95) with Microsoft SMTP Server id 7, 46 -- 14.1.225.22; Thu, 2 Jan 2014 12:58:26 +0000 7, 46 -- Received: from mail160-co9 (localhost [127.0.0.1]) by 7, 46 -- mail160-co9-R.bigfish.com (Postfix) with ESMTP id 9B9CD240094 for 7, 46 -- {Microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 12:58:26 +0000 (UTC) 7, 46 -- X-Forefront-Antispam-Report: CIP:132.245.156.37;KIP:(null);UIP:(null);IPV:NLI;H:GRXPRD8010HT004.lamprd80.prod.outlook.com;RD:none;EFVD:NLI 7, 46 -- X-SpamScore: -1 7, 46 -- X-BigFish: PS-1(zz1443Izz1f42h2148h208ch1ee6h1de0h1d18h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah1fc6hzzz2fh109h2a8h839h944hd25hf0ah1220h1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh15d0h162dh1631h1758h18e1h1946h19b5h19ceh1ad9h1b0ah224fh1d07h1d0ch1d2eh1d3fh1de9h1dfeh1dffh1e1dh1fe8h1ff5h2216h22d0h2336h1155h) 7, 46 -- Received-SPF: pass (mail160-co9: domain of fiemg.com.br designates 132.245.156.37 as permitted sender) client-ip=132.245.156.37; envelope-from=margareth.andrade-at-fiemg.com.br; helo=GRXPRD8010HT004.lamprd80.prod.outlook.com ;.outlook.com ; 7, 46 -- Received: from mail160-co9 (localhost.localdomain [127.0.0.1]) by mail160-co9 7, 46 -- (MessageSwitch) id 1388667503935090_11462; Thu, 2 Jan 2014 12:58:23 +0000 7, 46 -- (UTC) 7, 46 -- Received: from CO9EHSMHS009.bigfish.com (unknown [10.236.132.227]) by 7, 46 -- mail160-co9.bigfish.com (Postfix) with ESMTP id D317A38004C for 7, 46 -- {Microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 12:58:23 +0000 (UTC) 7, 46 -- Received: from GRXPRD8010HT004.lamprd80.prod.outlook.com (132.245.156.37) by 7, 46 -- CO9EHSMHS009.bigfish.com (10.236.130.19) with Microsoft SMTP Server (TLS) id 7, 46 -- 14.16.227.3; Thu, 2 Jan 2014 12:58:18 +0000 7, 46 -- Received: from GRXPRD8010MB526.lamprd80.prod.outlook.com ([169.254.10.185]) by 7, 46 -- GRXPRD8010HT004.lamprd80.prod.outlook.com ([10.242.26.37]) with mapi id 7, 46 -- 14.16.0395.000; Thu, 2 Jan 2014 12:58:11 +0000 7, 46 -- From: Margareth Spangler Andrade {margareth.andrade-at-fiemg.com.br} 7, 46 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 46 -- Subject: info on new JEOL 7, 46 -- Thread-Topic: info on new JEOL 7, 46 -- Thread-Index: Ac8HWS/Tlwh1lgE9TtWXDZDN2Ktc4AAYCnES 7, 46 -- Date: Thu, 2 Jan 2014 12:58:11 +0000 7, 46 -- Message-ID: {F96AE27C3117434CB0E8CB6C2EE9DA5429D81BF3-at-GRXPRD8010MB526.lamprd80.prod.outlook.com} 7, 46 -- References: {F96AE27C3117434CB0E8CB6C2EE9DA5429D81BC7-at-GRXPRD8010MB526.lamprd80.prod.outlook.com} 7, 46 -- In-Reply-To: {F96AE27C3117434CB0E8CB6C2EE9DA5429D81BC7-at-GRXPRD8010MB526.lamprd80.prod.outlook.com} 7, 46 -- Accept-Language: pt-BR, en-US 7, 46 -- Content-Language: pt-BR 7, 46 -- X-MS-Has-Attach: 7, 46 -- X-MS-TNEF-Correlator: 7, 46 -- x-originating-ip: [201.80.92.47] 7, 46 -- Content-Type: text/plain; charset="us-ascii" 7, 46 -- MIME-Version: 1.0 7, 46 -- X-OriginatorOrg: fiemg.com.br 7, 46 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 7, 46 -- Content-Transfer-Encoding: 8bit 7, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s02CwVCl015853 ==============================End of - Headers==============================
The GIF on our CM200FEG is now refusing to tune its isochromatic surface. I tried all of the obvious easy fixes, such as making sure I was on vacuum, acquiring a new gain reference, acquiring a new dark reference, reloading the last known good GIB alignment (~3 weeks old), and rebooting everything that can be booted (PC, GIB, Digiscan).
The error message is along the lines of "Too many defects in the isochromatic surface."
Any thoughts on what I might try? It's conceivable that a 20-year-old GIF has decided to fail; any way I could diagnose if a DAC or lens has decided to die on us?
Thanks Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 8, 30 -- From parishcm-at-ornl.gov Thu Jan 2 14:18:13 2014 8, 30 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s02KICpE009801 8, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 14:18:12 -0600 8, 30 -- X-SG: RELAYLIST 8, 30 -- X-IronPort-AV: E=Sophos;i="4.95,592,1384318800"; 8, 30 -- d="scan'208";a="53361480" 8, 30 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 8, 30 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 02 Jan 2014 15:18:11 -0500 8, 30 -- Received: from exchhub2.ornl.gov (exchhub2.ornl.gov [160.91.2.112]) 8, 30 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 8A4AEE0296 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Jan 2014 15:18:11 -0500 (EST) 8, 30 -- Received: from EXCHMBB.ornl.gov ([192.168.3.201]) by exchhub2.ornl.gov 8, 30 -- ([160.91.2.112]) with mapi; Thu, 2 Jan 2014 15:18:11 -0500 8, 30 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 8, 30 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 8, 30 -- Date: Thu, 2 Jan 2014 15:18:10 -0500 8, 30 -- Subject: EFTEM: GIF 678 won't tune 8, 30 -- Thread-Topic: EFTEM: GIF 678 won't tune 8, 30 -- Thread-Index: Ac8H98Bgh2q5tr7kTZOlENmCkwonHw== 8, 30 -- Message-ID: {3C47B404D340EE48A469ADAE2ADBF96676C1492CE6-at-EXCHMBB.ornl.gov} 8, 30 -- Accept-Language: en-US 8, 30 -- Content-Language: en-US 8, 30 -- X-MS-Has-Attach: 8, 30 -- X-MS-TNEF-Correlator: 8, 30 -- acceptlanguage: en-US 8, 30 -- Content-Type: text/plain; charset="us-ascii" 8, 30 -- MIME-Version: 1.0 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s02KICpE009801 ==============================End of - Headers==============================
Thanks to Pang, who had an eyepiece with a reticle. I just had to walk across campus and take it apart. The Periplan GF takes a reticle of 18.5 mm diameter inserted into the focusing eyepiece, not the fixed focus eyepiece. Remove the lower lens assembly (black) and then unscrew a small threaded ring from the “upper” end of the assembly. the reticle nests on a recessed ledge and held in place by the threaded ring.
Cynthia’s links are very useful for these older Leitz scopes.
Regards, Glen Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Cellular Morphology Core Center on Human Development and Disability Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-uw.edu
--------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } A colleague has a gray Leitz inverted microscope with Periplan GF 12.5X eyepieces, 23.2 mm outside diameter. Does anyone know whether they will take an eyepiece reticle? } Thanks, } Glen MacDonald } Core for Communication Research } Virginia Merrill Bloedel Hearing Research Center } Cellular Morphology Core } Center on Human Development and Disability } Box 357923 } University of Washington } Seattle, WA 98195-7923 USA } (206) 616-4156 } glenmac-at-uw.edu } } } } } } } } } } ==============================Original Headers============================== } 9, 27 -- From glenmac-at-u.washington.edu Tue Dec 31 10:44:24 2013 } 9, 27 -- Received: from mxout12.cac.washington.edu (mxout12.cac.washington.edu [140.142.32.167]) } 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id rBVGiO2e030605 } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 31 Dec 2013 10:44:24 -0600 } 9, 27 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.170]) } 9, 27 -- by mxout12.cac.washington.edu (8.14.4+UW11.03/8.14.4+UW13.09) with ESMTP id rBVGextf024220 } 9, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 31 Dec 2013 08:40:59 -0800 } 9, 27 -- X-Auth-Received: from d-128-208-204-174.dhcp4.washington.edu (D-128-208-204-174.dhcp4.washington.edu [128.208.204.174]) } 9, 27 -- (authenticated authid=glenmac) } 9, 27 -- by smtp.washington.edu (8.14.4+UW11.03/8.14.4+UW13.09) with ESMTP id rBVGew7H031750 } 9, 27 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 31 Dec 2013 08:40:58 -0800 } 9, 27 -- From: Glen MacDonald {glenmac-at-u.washington.edu} } 9, 27 -- Content-Type: text/plain; charset=us-ascii } 9, 27 -- Subject: Leitz periplan reticles } 9, 27 -- Message-Id: {EA6FCF59-6721-4EB0-A86E-A3255006651A-at-u.washington.edu} } 9, 27 -- Date: Tue, 31 Dec 2013 08:40:57 -0800 } 9, 27 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} } 9, 27 -- Mime-Version: 1.0 (Mac OS X Mail 7.0 \(1822\)) } 9, 27 -- X-Mailer: Apple Mail (2.1822) } 9, 27 -- X-PMX-Version: 6.0.0.2142326, Antispam-Engine: 2.7.2.2107409, Antispam-Data: 2013.12.31.163318 } 9, 27 -- X-PMX-Server: mxout12.cac.washington.edu } 9, 27 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report=' } 9, 27 -- HTML_00_01 0.05, HTML_00_10 0.05, MIME_LOWER_CASE 0.05, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_400_499 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, __ANY_URI 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_FROM 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __MSGID_APPLEMAIL 0, __SANE_MSGID 0, __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NO_PATH 0, __URI_NO_WWW 0, __URI_NS , __USER_AGENT_APPLEMAIL 0, __X_MAILER_APPLEMAIL 0' } 9, 27 -- Content-Transfer-Encoding: 8bit } 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id rBVGiO2e030605 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 29 -- From glenmac-at-u.washington.edu Thu Jan 2 14:24:03 2014 9, 29 -- Received: from mxout14.cac.washington.edu (mxout14.cac.washington.edu [140.142.32.168]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s02KO2o2014492 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 14:24:03 -0600 9, 29 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.205]) 9, 29 -- by mxout14.cac.washington.edu (8.14.4+UW11.03/8.14.4+UW13.09) with ESMTP id s02KNq9f013486 9, 29 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 12:23:52 -0800 9, 29 -- X-Auth-Received: from d-128-208-187-41.dhcp4.washington.edu (D-128-208-187-41.dhcp4.washington.edu [128.208.187.41]) 9, 29 -- (authenticated authid=glenmac) 9, 29 -- by smtp.washington.edu (8.14.4+UW11.03/8.14.4+UW13.09) with ESMTP id s02KNpEC027040 9, 29 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 12:23:52 -0800 9, 29 -- Content-Type: text/plain; charset=windows-1252 9, 29 -- Mime-Version: 1.0 (Mac OS X Mail 7.0 \(1822\)) 9, 29 -- Subject: Re: [Microscopy] Leitz periplan reticles 9, 29 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 9, 29 -- In-Reply-To: {201312311646.rBVGkOnC032160-at-ns.microscopy.com} 9, 29 -- Date: Thu, 2 Jan 2014 12:23:51 -0800 9, 29 -- Message-Id: {011012E8-2A7F-4D0B-8C77-3BF570D951B4-at-u.washington.edu} 9, 29 -- References: {201312311646.rBVGkOnC032160-at-ns.microscopy.com} 9, 29 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 9, 29 -- X-Mailer: Apple Mail (2.1822) 9, 29 -- X-PMX-Version: 6.0.0.2142326, Antispam-Engine: 2.7.2.2107409, Antispam-Data: 2014.1.2.201515 9, 29 -- X-PMX-Server: mxout14.cac.washington.edu 9, 29 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report=' 9, 29 -- HTML_00_01 0.05, HTML_00_10 0.05, MIME_LOWER_CASE 0.05, SUPERLONG_LINE 0.05, BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, URI_ENDS_IN_HTML 0, __ANY_URI 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FORWARDED_MSG 0, __HAS_FROM 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __IN_REP_TO 0, __LINES_OF_YELLING 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __MSGID_APPLEMAIL 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __SUBJ_ALPHA_END 0, __SUBJ_ALPHA_NEGATE 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS , __USER_AGENT_APPLEMAIL 0, __X_MAILER_APPLEMAIL 0' 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s02KO2o2014492 ==============================End of - Headers==============================
There are two possibilities, based on my experience with GIFs (I've used 5 different GIFs on 5 different instruments over the past 18 years):
1. The table of defective columns and rows has been lost (or modified) so that those genuinely defective pixels has not been correctly tabulated;
2. The Peltier cooler on the CCD camera is not cooling (at all).
On the balance of probability, I would suggest you investigate the second possibility first (the first would take some effort either by accident or malicious design).
We recently had problems with a GIF model 2000 on a CM300F which was commissioned in 1997. We had the same (or similar) error message. After contacting Gatan, they suggested estimating the temperature of the chip based on the dark count rate of two different exposure times (0.1 sec and 5.1 sec I think), taking the log of that ratio and dividing by some number (I cannot recall the exact figure- it is in my lab book back in the office- I'll try to dig it out when I return).
After this investigation, we found the Peltier cooler wasn't cooling the CCD chip. This was traced to a delicate copper wire that had broken that connected the Peltier PSU to the cooler. Gatan succeeded in fixing it (and getting the cooler to work at their offices), but did not work once it was reinstalled (we suspect the wire had broken again in transit). It is an open question as to whether repairing this again will fix the problem.
Anyway, I hope this helps in some small way.
Yours, Jon
Cambridge University, UK.
On 2014-01-02 20:27, parishcm-at-ornl.gov wrote:
} The GIF on our CM200FEG is now refusing to tune its isochromatic } surface. I tried all of the obvious easy fixes, such as making sure I } was on vacuum, acquiring a new gain reference, acquiring a new dark } reference, reloading the last known good GIB alignment (~3 weeks old), } and rebooting everything that can be booted (PC, GIB, Digiscan). } } The error message is along the lines of "Too many defects in the } isochromatic surface." } } Any thoughts on what I might try? It's conceivable that a 20-year-old } GIF has decided to fail; any way I could diagnose if a DAC or lens has } decided to die on us?
==============================Original Headers============================== 13, 31 -- From jsb43-at-cam.ac.uk Fri Jan 3 12:39:27 2014 13, 31 -- Received: from ppsw-52.csi.cam.ac.uk (ppsw-52.csi.cam.ac.uk [131.111.8.152]) 13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s03IdOr5031223 13, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Jan 2014 12:39:25 -0600 13, 31 -- X-Cam-AntiVirus: no malware found 13, 31 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 13, 31 -- Received: from webmail-2b.csi.cam.ac.uk ([131.111.9.35]:43068) 13, 31 -- by ppsw-52.csi.cam.ac.uk (ppsw.cam.ac.uk [131.111.8.138]:25) 13, 31 -- with esmtp id 1Vz9eV-0008Ge-FA (Exim 4.82_3-c0e5623) 13, 31 -- (return-path {jsb43-at-cam.ac.uk} ); Fri, 03 Jan 2014 18:39:23 +0000 13, 31 -- Received: from [127.0.0.1] (helo=webmail-2.hermes.cam.ac.uk) 13, 31 -- by webmail-2b.csi.cam.ac.uk with esmtp id 1Vz9eV-000qMM-KL (Exim 4.80_167-5a66dd3) 13, 31 -- (return-path {jsb43-at-cam.ac.uk} ); Fri, 03 Jan 2014 18:39:23 +0000 13, 31 -- Received: from host31-54-55-223.range31-54.btcentralplus.com ([31.54.55.223]) 13, 31 -- by webmail-2.hermes.cam.ac.uk 13, 31 -- with HTTP (HTTP/1.1 POST); Fri, 03 Jan 2014 18:39:23 +0000 13, 31 -- MIME-Version: 1.0 13, 31 -- Content-Type: text/plain; charset=UTF-8; 13, 31 -- format=flowed 13, 31 -- Content-Transfer-Encoding: 7bit 13, 31 -- X-Authenticated-User: jsb43 13, 31 -- Date: Fri, 03 Jan 2014 18:39:23 +0000 13, 31 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 13, 31 -- To: parishcm-at-ornl.gov 13, 31 -- Cc: Listserver MSA {Microscopy-at-microscopy.com} 13, 31 -- Subject: Re: EFTEM: GIF 678 won't tune 13, 31 -- In-Reply-To: {201401022027.s02KRo7r024182-at-ns.microscopy.com} 13, 31 -- References: {201401022027.s02KRo7r024182-at-ns.microscopy.com} 13, 31 -- Message-ID: {05d76d09370056d66680b787c8127d21-at-cam.ac.uk} 13, 31 -- X-Sender: jsb43-at-cam.ac.uk 13, 31 -- User-Agent: Roundcube Webmail/0.9.4 ==============================End of - Headers==============================
We are pleased to announce that the first meeting of the Chesapeake Microscopy and Microanalysis Society will be held on January 14th, 2014. We are very excited to have Dale Greenwalt of the Smithsonian National Museum of Natural History as our speaker that evening. If you are in the area of Bethesda MD, please come and have dinner with us. A flyer with all the information can be found on our web site (link below). We are looking for RSVPs by January 10 for our buffet at Dave and Buster's in White Flint Mall.
Agenda: 5:00-6:00 social hour with cash bar - 6:00-7:00 Dinner
7:00-8:00 Seminar - 8:00-8:30 Business Meeting.
Please RSVP to meetings-at-csmicroscopy.org so that we can have a headcount.
Cost: $25, cash/check at the door www.csmicroscopy.org
Happy New Year Chris
Christine A. Brantner, PhD [C] NIH NHLBI Electron Microscopy Core Facility National Institutes of Health 14 Service Road West Building 14E Room 104 MSC5570 Bethesda, MD 20892-5570
The notes quite easily on calculating the CCD temperature (dated 9th Nov 2011):
Step 1. Take two gain normalized images at 0.1 sec and 5.1 sec (blanked beam) and measure the average count rates per pixel (I_0.1 & I_5.1).
Step 2. Calculate the following quotient, q:
q = 10.037 * ln ((I_5.1 - I_0.1)/65.11424)
Where ln is the natural logarithm.
Step 3. The CCD temperature, T, in Kelvin is given by:
T = 20.0*(25 - q)
Example: I measured a difference in count rate of I_5.1 - I_0.1 = 209.9 counts per pixel, giving me a quotient of 11.748 and temperature of 265K (-8C).
I tried this several times to get some consistency: 217.7 counts =} -15.3C, etc.
I will try to find out exactly where these figures come from and update you if needed.
I hope this helps.
Yours, Jon
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A Healthy and Prosperous New Year to you, and the entire list.
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Best Regards, Darrell
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*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
realname - Liz Smith Email - elizabeth.smith.ctr-at-dha.mil ORGANIZATION - Defense Health Agency EDUCATION - Graduate College LOCATION - Falls Church, VA, USA SUBJECT_OF_QUESTION - Microscopy Finding - Definition QUESTION - To Whom It May Concern,
Microscopy Finding is a named, undefined attribute within Microbe Identification the Federal Health Information Model. Would you be able to help us with an authoritative definition for this term.
Respectfully, Liz Smith.
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Email: asw-at-sonnax.com Name: Amy Wall
Organization: Sonnax Industries
Title-Subject: [Filtered] Connect Nikon Epiphot 300 to digital camera
Message: Does anyone have experience connecting a Nikon Epiphot 300 metallograph to a digital camera? We are looking at a used Epiphot 300 and want to take pictures with a digital camera. The info I see says its simple to connect a digital camera to a side port but I find no further info on this "simple" connection. Thank you!
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Colleagues, I hope everyone had a relaxing break. I am about to embark on a fairly substantial project to characterize plant cell wall structure with immunogold labeling and imaging in FESEM. I have a few questions before investing in reagents.
I like the idea of using secondaries conjugated to ~1 nm gold followed by enhancement. How well does this work in practice? For my application I am not worried about 'too much' labeling and in fact I would like to see as much of the epitope as is present in the sample. I gather that there are both silver as well as gold enhancers. Anyone compare them? How does the 'shelf life' compare between a 10 nm- and a 1 nm- conjugate?
I will be labeling the surface of sectioned plant stems, so I am not worried about penetration through the sample. But I do wonder whether plant tissues / cell walls interact strangely with enhancement regents. Any experience?
Finally, and in general, I would appreciate comments on recent (last few years?) experiences with particular products/vendors.
Many many thanks, And those in the northern hemisphere stay warm!
Tobias -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Professor /_ / __ /__ \ \ \__ Biology Department / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / ___ / \ \__/ \ ____ Amherst, MA, 01003 www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 6, 18 -- From baskin-at-bio.umass.edu Tue Jan 7 20:35:57 2014 6, 18 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s082Zu24023733 6, 18 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jan 2014 20:35:56 -0600 6, 18 -- Received: from [10.0.1.2] (ip-159.250.64.67.dsl.crocker.net [159.250.64.67]) 6, 18 -- (authenticated bits=0) 6, 18 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id s082ZrYr019831 6, 18 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 18 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jan 2014 21:35:55 -0500 (EST) 6, 18 -- Mime-Version: 1.0 6, 18 -- Message-Id: {p0624057bcef2668fc923-at-[10.0.1.2]} 6, 18 -- Date: Tue, 7 Jan 2014 21:35:52 -0500 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 6, 18 -- Subject: immunogold for T or SEM 6, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 18 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Tue, 07 Jan 2014 21:35:55 -0500 (EST) 6, 18 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
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Organization: Sofia University, Faculty of Physics
Title-Subject: [Filtered] SEM Hitachi S570 documentation
Message: We have in the lab a Hitachi S570 SEM and we want to upgrade it. I am looking for the schematic documentation of the microscope column. I need to know the exact dimensions and separation distances in the column.
Can anyone help me to find it?
Thank you in advance.
G. Tsutsumanova
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I'm looking for TEM grid, 3.05 mm diameter, 200 mesh, carbon coated (about 200 A carbon thick) absolutely without impurity of Zn and Ni because I have to investigate (by EDS-TEM) on synthetic particles containing either Zn as Ni.
I already tried the Au, Cu, plastic grids and all contain some amount of at least one of these elements. I verified that the grids sold as Cu-grid contain either Ni as Zn. Do you have info on carbon coated grids with the characteristics I need?
I hope you can help me because I don't find a solution for this problem. I thank you a lot.
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Title-Subject: [Filtered] TEM sample preparation for Yeast and Fungi.
Message: Dear Listeners, How to prepare the yeast and fungi sample for TEM. Is there any other resin used instead of araldite.?
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==============================Original Headers============================== 14, 29 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 9 07:19:41 2014 14, 29 -- Received: from znl.com ([206.69.208.20]) 14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s09DJfD0016672 14, 29 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 07:19:41 -0600 14, 29 -- Received: from localhost (localhost [127.0.0.1]) 14, 29 -- by znl.com (Postfix) with ESMTP id D497F6A4B75 14, 29 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 07:19:41 -0600 (CST) 14, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 29 -- Received: from znl.com ([127.0.0.1]) 14, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 29 -- with ESMTP id YbKZRxJdGvVM for {microscopy-at-microscopy.com} ; 14, 29 -- Thu, 9 Jan 2014 07:19:40 -0600 (CST) 14, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 29 -- by znl.com (Postfix) with ESMTPA id BCC596A4B65 14, 29 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 07:19:40 -0600 (CST) 14, 29 -- Message-ID: {52CEA1EC.3070402-at-microscopy.com} 14, 29 -- Date: Thu, 09 Jan 2014 07:19:40 -0600 14, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 14, 29 -- MIME-Version: 1.0 14, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 29 -- Subject: viaWWW:TEM sample preparation for Yeast and Fungi.,,Message: Dear 14, 29 -- Listeners, 14, 29 -- References: {201401090918.s099IQ89027272-at-ns.microscopy.com} 14, 29 -- In-Reply-To: {201401090918.s099IQ89027272-at-ns.microscopy.com} 14, 29 -- X-Forwarded-Message-Id: {201401090918.s099IQ89027272-at-ns.microscopy.com} 14, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would be surprised if the stray EDX signals were coming from your grids. They are generally quite pure (at least at the TEM EDX level). TEM EDX detection limits will be a few tenths of an at% and grid impurities will be more in the ppm level. Also, the fact that you are seeing stray signal from many different grids suggests that the grids may not be the source. The only time i've ever seen impurities by EDX was a silicone contaminated C film where the diffusion pump oil backstreamed into the chamber during the film preparation. That batch of films showed huge Si contamination.
So, where is the stray signal coming from? I would suspect that you are seeing fluorescence from hard x-rays (bremsstrahlung) generated when the beam hits the C2 aperture. The bremsstrahlung will cause areas away from the sample in the sample chamber to fluoresce. This can be tested by putting the beam through a hole in the sample (called a "hole count"). In this case you should have no EDX signal at all. If you do see something, it is likely caused by the bremsstrahlung generated fluorescence.
Good luck, Henk
At 1/9/2014 5:57 AM, elena.belluso-at-unito.it wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Dear all, } } I'm looking for TEM grid, 3.05 mm diameter, 200 mesh, carbon coated } (about } 200 A carbon thick) absolutely without impurity of Zn and Ni because I } have } to investigate (by EDS-TEM) on synthetic particles containing either Zn } as } Ni. } } I already tried the Au, Cu, plastic grids and all contain some amount } of at } least one of these elements. I verified that the grids sold as Cu-grid } contain either Ni as Zn. Do you have info on carbon coated grids with } the } characteristics I need? } } I hope you can help me because I don't find a solution for this } problem. I } thank you a lot. } } Elena Belluso } } ------------------------------------------------------------------------------------ } } Elena BELLUSO } } Associate Professor in Environmental Mineralogy } } Ph.D. Mineralogy and Crystallography } } Dip. di Scienze della Terra, Universita' degli Studi di Torino } } Via Valperga Caluso n. 35, 10125 TORINO - ITALIA } } tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 } } e-mail: elena.belluso-at-unito.it [1] } } ------------------------------------------------------------------------------------ } } Elena BELLUSO } } Associate Professor in Environmental Mineralogy } } Ph.D. Mineralogy and Crystallography } } Dip. di Scienze della Terra, Universita' degli Studi di Torino } } Via Valperga Caluso n. 35, 10125 TORINO - ITALIA } } tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 } } e-mail: elena.belluso-at-unito.it [2] } } ------------------------------------------------------------------------------------ } } _"I've... seen things you people wouldn't believe. _ } } _Attack ships on fire off the shoulder of Orion. _ } } _I watched C-beams... glitter in the dark near the Tanhauser Gate. _ } } _All those... moments will be lost... in time..., _ } } _like... tears... in... rain." _ } } _Blade Runner_ } } } Links: } ------ } [1] mailto:elena.belluso-at-unito.it } [2] mailto:elena.belluso-at-unito.it } } ==============================Original Headers============================== } 32, 31 -- From elena.belluso-at-unito.it Thu Jan 9 04:55:48 2014 } 32, 31 -- Received: from mail-out2.unito.it (mail-out2.unito.it [130.192.119.63]) } 32, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s09Atlvh028560 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 04:55:48 -0600 } 32, 31 -- Received: (from root-at-localhost) } 32, 31 -- by mail-out2.unito.it (8.14.4/8.13.4/Debian-3sarge1) id s09AtlKI011223 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 11:55:47 +0100 } 32, 31 -- Received: from webmail.unito.it (webmail-balancer.unito.it [130.192.119.33]) } 32, 31 -- (authenticated bits=0) } 32, 31 -- by mail-out2.unito.it (8.14.4/8.13.4/Debian-3sarge1) with ESMTP id s09AtWNC010400 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 11:55:35 +0100 } 32, 31 -- X-Icontrol: Sent by Inrete Icontrol } 32, 31 -- MIME-Version: 1.0 } 32, 31 -- Content-Type: text/plain; charset=UTF-8; } 32, 31 -- format=flowed } 32, 31 -- Content-Transfer-Encoding: 7bit } 32, 31 -- Date: Thu, 09 Jan 2014 11:55:32 +0100 } 32, 31 -- From: Elena Belluso {elena.belluso-at-unito.it} } 32, 31 -- To: {Microscopy-at-microscopy.com} } 32, 31 -- Subject: TEM grid without Zn and Ni } 32, 31 -- Disposition-Notification-To: Elena Belluso {elena.belluso-at-unito.it} } 32, 31 -- Message-ID: {f6d345d3a6d5445bdd859ad77475a0a4-at-unito.it} } 32, 31 -- X-Sender: elena.belluso-at-unito.it } 32, 31 -- User-Agent: Roundcube Webmail/0.6 } 32, 31 -- X-From-User: ebelluso-at-unito.it } 32, 31 -- X-IControl-Milter-Checked: yes (IControlServer: mail-out2 SrcIpType: UnTrusted SrcIpHeaderType: Undefined Authenticated: yes) } 32, 31 -- X-IControl-Milter-SPF-Checked: yes (IControlServer: mail-out2 HeloSPFType: none FromSPFType: none HeloHeaderSPFType: Undefined FromHeaderSPFType: none) } 32, 31 -- X-IControl-Milter-MD5SUM: a74f735de91a38b9d0ae2313a42824ef } 32, 31 -- X-Inrete-Amavisjob-Virus-Scanned: PDAmail Multiple Antivirus with ClamAv } 32, 31 -- X-Inrete-Amavisjob-Service-Runned: 6 (s09AtWNC010400) } 32, 31 -- X-Inrete-Amavisjob-Service-Disabled: No Service disabled (s09AtWNC010400) } ==============================End of - Headers============================== }
-- Untitled Document
Hendrik O. Colijn *C*enter for *E*lectron *M*icroscopy & *A*nalysi*S* 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
Hi Ravi, You can use the protocol from Janice Griffith et al. in Journal of Electron Microscopy 60(3); 211-216 (2011). This is for IEM but you can also use it to prepare the sample for plastic embedding.
Groeten, Hans The Netherlands Cancer Institute. Amsterdam.
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: donderdag 9 januari 2014 14:24 To: Hans Janssen
X-from: ravi.thakkar369-at-gmail.com ()
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Title-Subject: [Filtered] TEM sample preparation for Yeast and Fungi.
Message: Dear Listeners, How to prepare the yeast and fungi sample for TEM. Is there any other resin used instead of araldite.?
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Happy new year to you all! I would appreciate your comments on the new non-radioactive Uranyl Acetate replacement Stain (a substitute for uranyl acetate). How do you like this product? What's your staining time? I know this was discussed not long ago so email off list if you prefer. thanks, Beth
==============================Original Headers============================== 2, 21 -- From beth-at-plantbio.uga.edu Thu Jan 9 09:08:48 2014 2, 21 -- Received: from dogwood.plantbio.uga.edu (pbmail.plantbio.uga.edu [128.192.26.3]) 2, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s09F8mco009632 2, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 09:08:48 -0600 2, 21 -- X-Footer: cGxhbnRiaW8udWdhLmVkdQ== 2, 21 -- Received: from [128.192.145.94] ([128.192.145.94]) 2, 21 -- (authenticated user beth-at-plantbio.uga.edu) 2, 21 -- by dogwood.plantbio.uga.edu 2, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 2, 21 -- for microscopy-at-microscopy.com; 2, 21 -- Thu, 9 Jan 2014 10:08:44 -0500 2, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 2, 21 -- Content-Type: text/plain; charset=us-ascii 2, 21 -- Subject: Uranyl Acetate replacement Stain 2, 21 -- Date: Thu, 9 Jan 2014 10:08:43 -0500 2, 21 -- Message-Id: {8CB76706-C8B1-46FD-B773-5C9FC3F921B1-at-plantbio.uga.edu} 2, 21 -- To: microscopy microscopy {microscopy-at-microscopy.com} 2, 21 -- Mime-Version: 1.0 (Apple Message framework v1085) 2, 21 -- X-Mailer: Apple Mail (2.1085) 2, 21 -- Content-Transfer-Encoding: 8bit 2, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s09F8mco009632 ==============================End of - Headers==============================
You may wish to consider using SiN windows mounted on thin Si wafers. These are available at most EM Supply houses. I have used these for supporting small particles and have successfully done XEDS on these for years. You will, of course, detect Si, N, and some O. I have never seen Zn or Ni with these SiN films in instruments here at the ANL EMCenter.
Look for Si wafers ~ 100 microns thick, rather than the thicker (200 -300 micron) ones. The SiN windows range from 10-100 nm, obviously the thinner the support window the better, but admittedly the 10nm thick windows are fragile. Just be careful, or start with thicker windows and work your way down.
Finally, be cognizant of which side you deposit your particles and also which side is facing your XEDS detector, in order that you do not get any shadowing effects due to the penumbra of the stage and/or the chemically etched Si surface.
Cheers,
Nestor Your Friendly Neighborhood SysOp
On 1/9/14 4:55 AM, elena.belluso-at-unito.it wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Dear all, } } I'm looking for TEM grid, 3.05 mm diameter, 200 mesh, carbon coated } (about } 200 A carbon thick) absolutely without impurity of Zn and Ni because I } have } to investigate (by EDS-TEM) on synthetic particles containing either Zn } as } Ni. } } I already tried the Au, Cu, plastic grids and all contain some amount } of at } least one of these elements. I verified that the grids sold as Cu-grid } contain either Ni as Zn. Do you have info on carbon coated grids with } the } characteristics I need? } } I hope you can help me because I don't find a solution for this } problem. I } thank you a lot. } } Elena Belluso } } ------------------------------------------------------------------------------------ } } Elena BELLUSO } } Associate Professor in Environmental Mineralogy } } Ph.D. Mineralogy and Crystallography } } Dip. di Scienze della Terra, Universita' degli Studi di Torino } } Via Valperga Caluso n. 35, 10125 TORINO - ITALIA } } tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 } } e-mail: elena.belluso-at-unito.it [1] } } ------------------------------------------------------------------------------------ } } Elena BELLUSO } } Associate Professor in Environmental Mineralogy } } Ph.D. Mineralogy and Crystallography } } Dip. di Scienze della Terra, Universita' degli Studi di Torino } } Via Valperga Caluso n. 35, 10125 TORINO - ITALIA } } tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 } } e-mail: elena.belluso-at-unito.it [2] } } ------------------------------------------------------------------------------------ } } _"I've... seen things you people wouldn't believe. _ } } _Attack ships on fire off the shoulder of Orion. _ } } _I watched C-beams... glitter in the dark near the Tanhauser Gate. _ } } _All those... moments will be lost... in time..., _ } } _like... tears... in... rain." _ } } _Blade Runner_ } } } Links: } ------ } [1] mailto:elena.belluso-at-unito.it } [2] mailto:elena.belluso-at-unito.it } } ==============================Original Headers============================== } 32, 31 -- From elena.belluso-at-unito.it Thu Jan 9 04:55:48 2014 } 32, 31 -- Received: from mail-out2.unito.it (mail-out2.unito.it [130.192.119.63]) } 32, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s09Atlvh028560 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 04:55:48 -0600 } 32, 31 -- Received: (from root-at-localhost) } 32, 31 -- by mail-out2.unito.it (8.14.4/8.13.4/Debian-3sarge1) id s09AtlKI011223 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 11:55:47 +0100 } 32, 31 -- Received: from webmail.unito.it (webmail-balancer.unito.it [130.192.119.33]) } 32, 31 -- (authenticated bits=0) } 32, 31 -- by mail-out2.unito.it (8.14.4/8.13.4/Debian-3sarge1) with ESMTP id s09AtWNC010400 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 11:55:35 +0100 } 32, 31 -- X-Icontrol: Sent by Inrete Icontrol } 32, 31 -- MIME-Version: 1.0 } 32, 31 -- Content-Type: text/plain; charset=UTF-8; } 32, 31 -- format=flowed } 32, 31 -- Content-Transfer-Encoding: 7bit } 32, 31 -- Date: Thu, 09 Jan 2014 11:55:32 +0100 } 32, 31 -- From: Elena Belluso {elena.belluso-at-unito.it} } 32, 31 -- To: {Microscopy-at-microscopy.com} } 32, 31 -- Subject: TEM grid without Zn and Ni } 32, 31 -- Disposition-Notification-To: Elena Belluso {elena.belluso-at-unito.it} } 32, 31 -- Message-ID: {f6d345d3a6d5445bdd859ad77475a0a4-at-unito.it} } 32, 31 -- X-Sender: elena.belluso-at-unito.it } 32, 31 -- User-Agent: Roundcube Webmail/0.6 } 32, 31 -- X-From-User: ebelluso-at-unito.it } 32, 31 -- X-IControl-Milter-Checked: yes (IControlServer: mail-out2 SrcIpType: UnTrusted SrcIpHeaderType: Undefined Authenticated: yes) } 32, 31 -- X-IControl-Milter-SPF-Checked: yes (IControlServer: mail-out2 HeloSPFType: none FromSPFType: none HeloHeaderSPFType: Undefined FromHeaderSPFType: none) } 32, 31 -- X-IControl-Milter-MD5SUM: a74f735de91a38b9d0ae2313a42824ef } 32, 31 -- X-Inrete-Amavisjob-Virus-Scanned: PDAmail Multiple Antivirus with ClamAv } 32, 31 -- X-Inrete-Amavisjob-Service-Runned: 6 (s09AtWNC010400) } 32, 31 -- X-Inrete-Amavisjob-Service-Disabled: No Service disabled (s09AtWNC010400) } ==============================End of - Headers============================== }
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Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Bldg 212 / A-143 9700 S. Cass Ave Argonne, Illinois 60439 USA
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Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
For yeast cells, cryo-fixation works much better than conventional chemical fixation. You can either use self-pressurized rapid freezing method or high pressure freezing method. The advantage of self pressurized rapid freezing is that you do not need specialized equipment and it is very easy to do. I routinely use this method for processing yeast cells. Following cryo-fixation, you will need to do cryo-substitution and eventually embed yeast cells in resin.
You can find more information about self-pressurized rapid freezing in the manuscript shown below:
Leunissen JL, Yi H (2009) Self-pressurized rapid freezing (SPRF): a novel cryofixation method for specimen preparation in electron microscopy. J Microsc (Oxford) 235:2535.
Hong
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Title-Subject: [Filtered] Sample preparation for TEM
Message: Hi Everybody
How can I prepare a sample of ZnO thin film deposited on glass substrate by RF Magnetron sputtering, so i can analyze it in TEM
Is there any published method or somebody knows an easy way?
Thanks
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 9 18:48:42 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0A0mg7t011332 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:42 -0600 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id 994B46A50A4 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:42 -0600 (CST) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id JRtDDcrJAwqU for {microscopy-at-microscopy.com} ; 17, 28 -- Thu, 9 Jan 2014 18:48:41 -0600 (CST) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id B917B6A5097 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:41 -0600 (CST) 17, 28 -- Message-ID: {52CF4369.2080800-at-microscopy.com} 17, 28 -- Date: Thu, 09 Jan 2014 18:48:41 -0600 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Sample preparation ZnO for TEM 17, 28 -- References: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: mmcgough-at-histochemicalsociety.org Name: Meg McGough
Organization: Histochemical Society
Title-Subject: [Filtered] MBL Immunohistochemistry and Microscopy Course
Message: Registration is still open for the 2014 Immunohistochemistry and Microscopy Course (IHCM) at the Marine Biological Laboratory. Please alert your students, postdocs and colleagues to this opportunity to attend one of MBL's Special Topics course on Immunohistochemistry and Microscopy. Registration closes on Tuesday, January 14! Course dates are March 15-20, 2014.
The Immunohistochemistry and Microscopy (IHCM) course is four full days and evenings (11 hours daily) of lecture and laboratory sessions with experts in the field of immunohistochemistry (IHC) and microscopy. The IHCM course goal is to provide participants in-depth theory of and extensive hands-on experience with immunohistochemistry (IHC) techniques as well as theory and hands-on experience with a broad range of microscopic imaging techniques. The course emphasizes hands-on laboratory time and small breakout discussions with faculty and staff.
This course is appropriate for undergraduate and graduate students, laboratory technicians, postdoctoral students, new and established faculty/clinicians seeking to expand their techniques and knowledge of IHC and microscopy. It is appropriate for beginning scientists and those with more advanced skills. Participants will be grouped appropriately. Registration is limited to thirty.
Completed application forms and required supporting documentation must be received by January 14, 2014.
FASEB Marc Awards and HCS Travel Awards are available to support attendance at the course: http://histochemicalsociety.org/Awards.aspx
For further information about the course: http://hermes.mbl.edu/education/courses/special_topics/ihcm.html
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Email: info-at-nesmicroscopy.org Name: New England Society for Microscopy
Organization: New England Society for Microscopy
Title-Subject: [Filtered] Save the Date: NESM's Annual Spring Meeting -at- Cabot Corporation!
Message: Dear Fellow Microscopists,
Remember to save the date for the New England Society for MicroscopyÂ’s annual Spring Meeting on Tuesday, February 25th at Cabot Corporation! The meeting will consist of a buffet dinner and two technical talks. More information to follow when registration opens up in the coming weeks. Keep up with NESM by visiting our website (www.nesmicroscopy.org) and by following us on Facebook (http://www.facebook.com/NESMicroscopy) and on twitter (http://www.twitter.com/#!/NESMicroscopy).
We hope to see you in February!
Cheers, NESM Board
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I'd like to explore lab management software that includes calendar, user approval, account management, invoicing etc. I have found iLab, OCF, PPMS and Faces. Are their other products? I'd like to talk (off line) with those that use these products.
Disclaimer. I don't have a financial relationship with any of the vendors that distribute the products I mention in this message.
Thank you.
Owen
Owen P. Mills Director, Applied Chemical and Morphological Analysis Laboratory ACMAL Materials Science & Engineering Michigan Technological University 1400 Townsend Drive Houghton, MI 49931 PH 906-369-1875 FAX 906-487-2934 opmills-at-mtu.edu
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The easiest and fastest way would be to deposit your coating on a #0 or #1 glass cover plate and use a slightly modified version of the small angle cleavage technique. When you start scribing with the mini scribes, some pieces will have a small angle and will be good specimens when you mount them on the grid vertically. For ZnO, the transition region from equiaxed growth to columnar is weak and you often get a jog in the cross section of the film with the columnar structure back further from the tip. It just means that you have to screen more samples in the microscope. Get your hands on the MRS vol 480 book for Sample Prep IV and look at John McCaffrey and my paper.
The Small Angle Cleavage Technique: An Update, S. D. Walck and J. P. McCaffrey, Proceedings of the Materials Research Society, Workshop on Specimen Preparation for Transmission Electron Microscopy of Materials IV, eds. Ron M. Anderson and Scott D. Walck, Vol 480, Pittsburgh, (1997).
I think that we talk about ZnO results in this paper, but I'm not 100% positive: The Small Angle Cleavage Technique Applied to Coatings and Thin Films, S. D. Walck and J. P. McCaffrey, Thin Solid Films, 308-309, pp. 399-405, 1997.
- -Scott
Scott D. Walck, Ph.D. Sr. Scientist Bowhead Science & Technology U.S. Army Research Laboratory RDRL-WMM-C 4600 Deer Creek Loop APG, MD 21005-5069 USA
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Title-Subject: [Filtered] Sample preparation for TEM
Message: Hi Everybody
How can I prepare a sample of ZnO thin film deposited on glass substrate by RF Magnetron sputtering, so i can analyze it in TEM
Is there any published method or somebody knows an easy way?
Thanks
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 9 18:48:42 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0A0mg7t011332 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:42 -0600 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id 994B46A50A4 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:42 -0600 (CST) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id JRtDDcrJAwqU for {microscopy-at-microscopy.com} ; 17, 28 -- Thu, 9 Jan 2014 18:48:41 -0600 (CST) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id B917B6A5097 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:41 -0600 (CST) 17, 28 -- Message-ID: {52CF4369.2080800-at-microscopy.com} 17, 28 -- Date: Thu, 09 Jan 2014 18:48:41 -0600 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Sample preparation ZnO for TEM 17, 28 -- References: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 31, 36 -- From s.walck-at-comcast.net Sat Jan 11 13:36:11 2014 31, 36 -- Received: from qmta05.westchester.pa.mail.comcast.net (qmta05.westchester.pa.mail.comcast.net [76.96.62.48]) 31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0BJaAUs024374 31, 36 -- for {microscopy-at-microscopy.com} ; Sat, 11 Jan 2014 13:36:11 -0600 31, 36 -- Received: from omta06.westchester.pa.mail.comcast.net ([76.96.62.51]) 31, 36 -- by qmta05.westchester.pa.mail.comcast.net with comcast 31, 36 -- id Chcz1n00416LCl055jc8lA; Sat, 11 Jan 2014 19:36:08 +0000 31, 36 -- Received: from WalckPC ([IPv6:2601:a:80:4e1:64f7:a070:5cc2:96fa]) 31, 36 -- by omta06.westchester.pa.mail.comcast.net with comcast 31, 36 -- id Cjc81n00107uUYL3Sjc84A; Sat, 11 Jan 2014 19:36:08 +0000 31, 36 -- From: "Scott Walck" {S.Walck-at-comcast.net} 31, 36 -- To: {microscopy-at-microscopy.com} 31, 36 -- References: {201401100108.s0A18ano027725-at-ns.microscopy.com} 31, 36 -- In-Reply-To: {201401100108.s0A18ano027725-at-ns.microscopy.com} 31, 36 -- Subject: RE: [Microscopy] viaWWW:Sample preparation ZnO for TEM 31, 36 -- Date: Sat, 11 Jan 2014 14:36:05 -0500 31, 36 -- Message-ID: {017c01cf0f04$5e6c37b0$1b44a710$-at-comcast.net} 31, 36 -- MIME-Version: 1.0 31, 36 -- Content-Type: text/plain; 31, 36 -- charset="iso-8859-1" 31, 36 -- X-Mailer: Microsoft Outlook 14.0 31, 36 -- Thread-Index: AQItE8gNsZ350rWjfhi9XF8u27v9zpnD3uGA 31, 36 -- Content-Language: en-us 31, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 31, 36 -- s=q20121106; t=1389468968; 31, 36 -- bh=g+KGtoG+VLcYPXhWep2BmjCaioLG4mb5FckijAf+PuQ=; 31, 36 -- h=Received:Received:From:To:Subject:Date:Message-ID:MIME-Version: 31, 36 -- Content-Type; 31, 36 -- b=Aa+CaMZym4S5EcahM3ZFeVMcUvIUMQ0Aj9gRQnuAeGU8CBNAr3qOKu153BNgM+/AR 31, 36 -- 0ob3GiSl+c4MlNNxciREAfqA2LXPOz9eFZF4gPiS/+VU2Yt4YMOWz/6ymxp3hfvqtP 31, 36 -- uo2QAhOdON6FqBcr6UF2pPCN1zW61jmVFOZcFYu3L45zEQswTCS22dSyhI1wOhtDDl 31, 36 -- FZA5bifxc3Vj2zEx5UrXTSjxNSwiUaq93xVEndiOlrSUJ1A7MAec7Yp2DgoXUUnP9J 31, 36 -- wM+PdStC5CALm6eOXWMDIfArfLD5BkODia0zamfl8FY+uViXlQdv3ZQ2/lUgww1jtF 31, 36 -- tARefGKmDuhrQ== 31, 36 -- Content-Transfer-Encoding: 8bit 31, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0BJaAUs024374 ==============================End of - Headers==============================
We have a couple of Hitachi CuteVac VR16L-K roughing pumps that need new vanes and oil seals. Unfortunately, nobody seems to sell parts for these. A couple of people have suggested that the parts are interchangeable with another manufacturer, (Alcatel?) but nobody has been able to provide specific part #s for the vanes or anything else.
Anyone rebuilt one of these before and remember which vanes and seals to use?
Thanks, Bradford Ross
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-6996
==============================Original Headers============================== 13, 31 -- From bradford.ross-at-botany.ubc.ca Mon Jan 13 13:28:40 2014 13, 31 -- Received: from vmaprod3.mail-relay.ubc.ca (vmaprod3.mail-relay.ubc.ca [142.103.117.136]) 13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0DJSeBU001075 13, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 13:28:40 -0600 13, 31 -- Received: from vmaprod3.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 13, 31 -- by localhost (Email Security Appliance) with SMTP id DAA341CB5AB_2D43E67B 13, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 19:28:39 +0000 (GMT) 13, 31 -- Received: from smtp.mail.ubc.ca (s-itsv-hub04p.ead.ubc.ca [137.82.151.86]) 13, 31 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 13, 31 -- (Client CN "rpc.mail.ubc.ca", Issuer "Entrust Certification Authority - L1C" (verified OK)) 13, 31 -- by vmaprod3.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id A5B751CB5A0_2D43E67F 13, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 19:28:39 +0000 (GMT) 13, 31 -- Received: from S-ITSV-MBX05P.ead.ubc.ca ([169.254.12.43]) by 13, 31 -- s-itsv-hub04p.ead.ubc.ca ([137.82.151.86]) with mapi id 14.03.0174.001; Mon, 13, 31 -- 13 Jan 2014 11:28:39 -0800 13, 31 -- From: "Ross, Bradford" {bradford.ross-at-botany.ubc.ca} 13, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 13, 31 -- Subject: Hitachi RP Rebuild 13, 31 -- Thread-Topic: Hitachi RP Rebuild 13, 31 -- Thread-Index: Ac8QlWu5a75tm1e1Qe+heqfjHsrttw== 13, 31 -- Date: Mon, 13 Jan 2014 19:28:38 +0000 13, 31 -- Message-ID: {B48A2601A4664A4AAF5A3D2F0E48280101527164B5-at-s-itsv-mbx05p.ead.ubc.ca} 13, 31 -- Accept-Language: en-US, en-CA 13, 31 -- Content-Language: en-US 13, 31 -- X-MS-Has-Attach: 13, 31 -- X-MS-TNEF-Correlator: 13, 31 -- x-originating-ip: [137.82.85.253] 13, 31 -- Content-Type: text/plain; charset="us-ascii" 13, 31 -- MIME-Version: 1.0 13, 31 -- Content-Transfer-Encoding: 8bit 13, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0DJSeBU001075 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Registration Reminder: FIB SEM Workshop, Feb 27, 2014 and Johns Hopkins APL
Message: Call for Papers & Registration Reminder
7th Annual FIB SEM Workshop
Thursday, February 27, 2014
Kossiakoff Center
Johns Hopkins Applied Physics Laboratory
Laurel, MD
2014 FIB SEM Workshop will be held at the Kossiakoff Center at Johns Hopkins Applied Physics Laboratory (APL). We will have a full day of presentations and posters by FIB users and vendors highlighting interesting new FIB applications and the latest technology. And, as always, there will be plenty of opportunities for informal discussion of new techniques and applications as well as getting (re)connected with your fellow FIBers.
If you would like to present a paper or poster, please submit an abstract to the organizers. Starting this year, we are requiring an abstract submission from all presenters (user & vendor contributions). All FIB related topics such as new and novel application areas for FIB, advances in FIB and FIB related instrumentation and techniques, image and data processing techniques for FIB data are all welcome.
· Abstract Deadline: January 15, 2014
Abstracts should be 250 words or less in length. Email abstracts to fibsem2014-at-fibsem.net.
Abstract Notification: February 1, 2014
· Registration Deadline: February 3, 2014 - Register!
· Travel Info
Please check our website (www.fibsem.org) for additional information about the meeting. There is no fee to attend the meeting, and breakfast and lunch will be provided! There will be a Happy Hour following the meeting, so donÂ’t forget to sign up for it when you register.
Remember, you don't have to be a local user to attend! We look forward to seeing you at APL!
Organizers:
Nabil Bassim, nabil.bassim-at-nrl.navy.mil
Ken Livi, klivi-at-jhu.edu
Joan Hoffmann, joan.hoffmann-at-jhuapl.edu
Keana Scott, keana.scott-at-nist.gov
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==============================Original Headers============================== 38, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 13 17:55:29 2014 38, 28 -- Received: from znl.com ([206.69.208.20]) 38, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0DNtTQb008982 38, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:55:29 -0600 38, 28 -- Received: from localhost (localhost [127.0.0.1]) 38, 28 -- by znl.com (Postfix) with ESMTP id 16D9C6A7E2A 38, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:55:29 -0600 (CST) 38, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 38, 28 -- Received: from znl.com ([127.0.0.1]) 38, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 38, 28 -- with ESMTP id MnL16QTMLXJ3 for {microscopy-at-microscopy.com} ; 38, 28 -- Mon, 13 Jan 2014 17:55:22 -0600 (CST) 38, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 38, 28 -- by znl.com (Postfix) with ESMTPA id 28AF76A7E1D 38, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:55:22 -0600 (CST) 38, 28 -- Message-ID: {52D47CE9.50205-at-microscopy.com} 38, 28 -- Date: Mon, 13 Jan 2014 17:55:21 -0600 38, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 38, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 38, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 38, 28 -- MIME-Version: 1.0 38, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 38, 28 -- Subject: viaWWW:FIB SEM Workshop, Feb 27, 2014 -at- Johns Hopkins APL 38, 28 -- References: {201401131623.s0DGNNBm030039-at-ns.microscopy.com} 38, 28 -- In-Reply-To: {201401131623.s0DGNNBm030039-at-ns.microscopy.com} 38, 28 -- X-Forwarded-Message-Id: {201401131623.s0DGNNBm030039-at-ns.microscopy.com} 38, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 38, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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You might want to obtain books written by Rick Giberson, since he is one of the inventors and pioneers for the microwave tissue processor. The microwave processor with vacuumm is one of the best inventions of the last 50 years. By using the microwave with vacuum you can now see organelles that you didn't know were there before. It is almost indispensible for lung or other tissues where you have lots of voids and spaces. We routinely use the technology on mouse and drosophila tissue. In the past I have used it on avian, reptile, and plant tissue also with excellent results. I am sure that if you call Ted Pella, Rick or spomeone would be happy to help you convert any protocols you may have.
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 13 17:56:28 2014 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0DNuSH5009940 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:56:28 -0600 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id 196236A7E39 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:56:28 -0600 (CST) 15, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id dLxU312YhYVA for {microscopy-at-microscopy.com} ; 15, 28 -- Mon, 13 Jan 2014 17:56:26 -0600 (CST) 15, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id D24936A7E2C 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:56:26 -0600 (CST) 15, 28 -- Message-ID: {52D47D2A.8070800-at-microscopy.com} 15, 28 -- Date: Mon, 13 Jan 2014 17:56:26 -0600 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:microwave processing 15, 28 -- References: {201401131943.s0DJh1Eo016083-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201401131943.s0DJh1Eo016083-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201401131943.s0DJh1Eo016083-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: mmcgough-at-histochemicalsociety.org Name: Meg McGough
Message: The application deadline for the 2014 Immunohistochemistry and Microscopy Course (IHCM) at the Marine Biological Laboratory has been extended until January 21. Course dates are March 15-20, 2014.
The Immunohistochemistry and Microscopy (IHCM) course is four full days and evenings (11 hours daily) of lecture and laboratory sessions with experts in the field of immunohistochemistry (IHC) and microscopy. The IHCM course goal is to provide participants in-depth theory of and extensive hands-on experience with immunohistochemistry (IHC) techniques as well as theory and hands-on experience with a broad range of microscopic imaging techniques. The course emphasizes hands-on laboratory time and small breakout discussions with faculty and staff.
This course is appropriate for undergraduate and graduate students, laboratory technicians, postdoctoral students, new and established faculty/clinicians seeking to expand their techniques and knowledge of IHC and microscopy. It is appropriate for beginning scientists and those with more advanced skills. Participants will be grouped appropriately. Registration is limited to thirty.
Completed application forms and required supporting documentation must be received by January 21, 2014.
FASEB Marc Awards are available to support attendance at the course: http://www.faseb.org/MARC-and-Professional-Development/Travel-Awards/Scientific-Meetings-and-Conferences.aspx
For further information about the course: http://hermes.mbl.edu/education/courses/special_topics/ihcm.html
Thank you, Meg McGough The Histochemical Society
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 13 17:58:34 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0DNwX4O012968 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:58:33 -0600 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 5A70B6A7E4C 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:58:33 -0600 (CST) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id rYSA58GupOYN for {microscopy-at-microscopy.com} ; 16, 28 -- Mon, 13 Jan 2014 17:58:31 -0600 (CST) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 1ACAB6A7E3E 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:58:31 -0600 (CST) 16, 28 -- Message-ID: {52D47DA6.1050902-at-microscopy.com} 16, 28 -- Date: Mon, 13 Jan 2014 17:58:30 -0600 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:MBL Immunohistochemistry and Microscopy Course 16, 28 -- References: {201401131934.s0DJYILh006048-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201401131934.s0DJYILh006048-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201401131934.s0DJYILh006048-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Elena, Have you considered that the Zn/Ni could be coming from the TEM or sample holder (or even the EDS detector) rather than the grid itself. When you hit the sample with high energy elections, you generate a huge number of x-rays of all energies in addition to backscattered electrons. If you have any brass near your sample, you are almost guaranteed to get Cu and Zn in your EDS spectrum from non-local fluorescence. The Ni could be coming from SS near the sample or the TEM pole piece (do you also see Fe?). While TEM based EDS is a lot cleaner than it once was, you can never really be sure where the x-rays that enter your detector originate. Using low background holders and TEM hard x-ray apertures (if available) can help. Chapter 33.3 in Williams and Carter's TEM book details these issues and describes precautions to help reduce system and spurious x-ray artifacts.
If you have access to an EELS detector, you can run the experiments without worrying about fluorescence artifacts. You will only see the element if it is under the beam.
Hope this helps.
-Ray
Disclaimer: I work for Gatan which provides EELS and EDS equipment and software as well as low background TEM sample holders.
Ray D. Twesten Livermore, CA
-----Original Message----- X-from: elena.belluso-at-unito.it [mailto:elena.belluso-at-unito.it] Sent: Thursday, January 09, 2014 3:04 AM To: ray.twesten-at-sbcglobal.net
Dear all,
I'm looking for TEM grid, 3.05 mm diameter, 200 mesh, carbon coated (about 200 A carbon thick) absolutely without impurity of Zn and Ni because I have to investigate (by EDS-TEM) on synthetic particles containing either Zn as Ni.
I already tried the Au, Cu, plastic grids and all contain some amount of at least one of these elements. I verified that the grids sold as Cu-grid contain either Ni as Zn. Do you have info on carbon coated grids with the characteristics I need?
I hope you can help me because I don't find a solution for this problem. I thank you a lot.
For the purpose of orientation while viewing a specimen in TEM, I naively thought it might be useful to "color" a resin by adding electrodense grains (e.g.ferritin) and then, after appropriate cutting, to re-embed the block in the "colored" resin. In this way one or more side(s) of the specimen touching the cut can be readily recognizable in the thin sections under the TEM.
Does it make any sense what I am saying and (if yes) is there any experience on this?
April 8, 2014 marks the end of support from Microsoft for Windows XP. That means no more security updates to patch newly discovered security holes. This will leave any computers still running Windows XP vulnerable to attacks if they remain connected to the internet or if infected memory media are inserted into the computer. I am sure there are a lot of machines out there running Windows XP. I am curious to hear what approaches microscopy community members are taking to deal with this, as well as what the community of microscopy related hardware vendors are doing to address this situation. Thank you.
John Mardinly, ASU
==============================Original Headers============================== 4, 35 -- From John.Mardinly-at-asu.edu Wed Jan 15 15:12:14 2014 4, 35 -- Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0FLCEU1008827 4, 35 -- for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 15:12:14 -0600 4, 35 -- X-ASG-Debug-ID: 1389820333-0549c456250a750001-FOsErg 4, 35 -- Received: from exhubw01.asurite.ad.asu.edu (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnetw3.asu.edu with ESMTP id Lezl3NXq5dB4vpTh (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 14:12:13 -0700 (MST) 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 4, 35 -- X-ASG-Whitelist: Client 4, 35 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 4, 35 -- exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id 14.02.0309.002; 4, 35 -- Wed, 15 Jan 2014 14:12:12 -0700 4, 35 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 35 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 4, 35 -- Subject: Windows XP Support Ending April 8, 2014 4, 35 -- Thread-Topic: Windows XP Support Ending April 8, 2014 4, 35 -- X-ASG-Orig-Subj: Windows XP Support Ending April 8, 2014 4, 35 -- Thread-Index: Ac8SNnTksb2ozlmjTzO6+BJCz2aDAQ== 4, 35 -- Date: Wed, 15 Jan 2014 21:12:11 +0000 4, 35 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400B7A2850-at-exmbt05.asurite.ad.asu.edu} 4, 35 -- Accept-Language: en-US 4, 35 -- Content-Language: en-US 4, 35 -- X-MS-Has-Attach: 4, 35 -- X-MS-TNEF-Correlator: 4, 35 -- x-originating-ip: [129.219.103.4] 4, 35 -- Content-Type: text/plain; charset="us-ascii" 4, 35 -- MIME-Version: 1.0 4, 35 -- X-Barracuda-Connect: exhubw01.asurite.ad.asu.edu[129.219.4.199] 4, 35 -- X-Barracuda-Start-Time: 1389820333 4, 35 -- X-Barracuda-Encrypted: AES128-SHA 4, 35 -- X-Barracuda-URL: http://149.169.2.73:8000/cgi-mod/mark.cgi 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 35 -- X-Barracuda-BRTS-Status: 1 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0FLCEU1008827 ==============================End of - Headers==============================
At my place, we are putting a Win7 computer between the XPs and the corporate network. The Win7 will act as a server for the XPs. We have a few SEMs and a couple of other tools that cannot be upgraded to Win7. Unless, of course, we want to buy whole new tools!
} -----Original Message----- } From: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] } Sent: Wednesday, January 15, 2014 3:12 PM } To: Holdford, Becky } Subject: [Microscopy] Windows XP Support Ending April 8, 2014 } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To
==============================Original Headers============================== 3, 32 -- From r-holdford-at-ti.com Wed Jan 15 17:50:40 2014 3, 32 -- Received: from devils.ext.ti.com (devils.ext.ti.com [198.47.26.153]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0FNoexB030568 3, 32 -- for {microscopy-at-microscopy.com} ; Wed, 15 Jan 2014 17:50:40 -0600 3, 32 -- Received: from dflxv15.itg.ti.com ([128.247.5.124]) 3, 32 -- by devils.ext.ti.com (8.13.7/8.13.7) with ESMTP id s0FNobgP008756; 3, 32 -- Wed, 15 Jan 2014 17:50:38 -0600 3, 32 -- Received: from DFLE72.ent.ti.com (dfle72.ent.ti.com [128.247.5.109]) 3, 32 -- by dflxv15.itg.ti.com (8.14.3/8.13.8) with ESMTP id s0FNobmc010160; 3, 32 -- Wed, 15 Jan 2014 17:50:37 -0600 3, 32 -- Received: from DLEE08.ent.ti.com ([fe80::b57d:c966:76e5:eb71]) by 3, 32 -- DFLE72.ent.ti.com ([fe80::e5e2:bab6:34dc:a483%28]) with mapi id 3, 32 -- 14.02.0342.003; Wed, 15 Jan 2014 17:50:37 -0600 3, 32 -- From: "Holdford, Becky" {r-holdford-at-ti.com} 3, 32 -- To: "John.Mardinly-at-asu.edu" {John.Mardinly-at-asu.edu} 3, 32 -- CC: "MSA Listerver (microscopy-at-microscopy.com)" {microscopy-at-microscopy.com} 3, 32 -- Subject: RE: [Microscopy] Windows XP Support Ending April 8, 2014 3, 32 -- Thread-Topic: [Microscopy] Windows XP Support Ending April 8, 2014 3, 32 -- Thread-Index: AQHPEjZ+7S3V71p2l0enBlFa2Pign5qGdABA 3, 32 -- Date: Wed, 15 Jan 2014 23:50:36 +0000 3, 32 -- Message-ID: {9128BB8114AB41469F2BAA1EA3A4F85D237086B2-at-DLEE08.ent.ti.com} 3, 32 -- References: {201401152112.s0FLCOuv009012-at-ns.microscopy.com} 3, 32 -- In-Reply-To: {201401152112.s0FLCOuv009012-at-ns.microscopy.com} 3, 32 -- Accept-Language: en-US 3, 32 -- Content-Language: en-US 3, 32 -- X-MS-Has-Attach: 3, 32 -- X-MS-TNEF-Correlator: 3, 32 -- x-originating-ip: [128.247.5.50] 3, 32 -- Content-Type: text/plain; charset="us-ascii" 3, 32 -- MIME-Version: 1.0 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0FNoexB030568 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sergey-at-seas.ucla.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
The Southern California Society for Microscopy and Microanalysis (SCSMM) announce their Full-Day Symposium. The meeting will be held on Saturday, February 8th from 8:00 am at Calit2 Auditorium at the University of California, Irvine. A lunch and coffee breaks will be served.
Our symposium, dedicated to hybrid approaches in microscopy. Beside the fact that it poses significant challenges for researchers in both life and materials sciences, it continues the conversation about nanoscience, its role, and its future. We are honored to have Prof. C. Barry Carter from the University of Connecticut best known in the “microscopy world” as the co-author of the textbook “Transmission Electron Microscopy: A Textbook for Materials Science” as our Microscopy Society of America Invited Speaker. He will have an inspiring talk titled “Challenges for TEM and TEMers: Remember the Past if You Can” in which he links the history of TEM to the current status of the field and its future. Along with Prof. Carter, our invited speakers Prof. Suneel Kodambaka (UCLA), Prof. Dorit Hanein (Sanford-Burnham MRI), Prof. Daniel R. Mumm (UCI) and Dr. Yi-Wei Chang (Caltech) will make our symposium a remarkable event.
In order to register, please RSVP by email at micromark-at-juno.com. Respond no later than 5:00 p.m. Friday, January 31st, 2014 (indicate your special needs by this due).
The cost is $25 for professionals and $10 for students. Membership dues can be paid at the door on February 8th, 2014.
Nonmembers are welcome to attend.
Please pass along to those you feel would be interested.
We look forward to seeing you at the meeting!
http://www.scsmm.org/
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==============================Original Headers============================== 21, 39 -- From nestor.zaluzec-at-gmail.com Wed Jan 15 21:26:17 2014 21, 39 -- Received: from mail-ie0-f174.google.com (mail-ie0-f174.google.com [209.85.223.174]) 21, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0G3QHWo018614 21, 39 -- for {microscopy-at-microscopy.com} ; Wed, 15 Jan 2014 21:26:17 -0600 21, 39 -- Received: by mail-ie0-f174.google.com with SMTP id lx4so3234332iec.19 21, 39 -- for {microscopy-at-microscopy.com} ; Wed, 15 Jan 2014 19:26:17 -0800 (PST) 21, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 21, 39 -- d=gmail.com; s=20120113; 21, 39 -- h=sender:message-id:date:from:reply-to:user-agent:mime-version:to 21, 39 -- :subject:references:in-reply-to:content-type 21, 39 -- :content-transfer-encoding; 21, 39 -- bh=q9pkbdLoFYERF3qnMTaLbZtCthn6ccMgbVVnP2X/ilg=; 21, 39 -- b=iFqmZayr/MG56l5Fj8kYfJKwXOJ6yWJV0IiATzJ79o8x/jg97Y99mCQEwxh72e16gn 21, 39 -- 4/ZN325cDRfxvmnAWbGfYo7QvampOMbUBVpBsfgltDYu5wMVl+HJ/pwamM2m4lJon3il 21, 39 -- sKPk/XUMZgUS69gBpWFziRwEqF3j6q4XWpM118RC8JaxJSDZ4SK73P+ODMoDjOHjFZJg 21, 39 -- LxgrZ3cLc8OcRpYbdvPtNCPcMnmpxiPrfiEb0aE8xPxGRgZL8u8bs/BLuIyz0DPk+WWe 21, 39 -- xS2yTD9kBoikuLVeBQZiwQcF6BvYZrmq8rr0FBpGBy9NQaDIJSVYk1GX9c/jwTaylJGX 21, 39 -- UATQ== 21, 39 -- X-Received: by 10.42.62.196 with SMTP id z4mr5637278ich.49.1389842777498; 21, 39 -- Wed, 15 Jan 2014 19:26:17 -0800 (PST) 21, 39 -- Received: from NestorPwrBk.local (adsl-99-141-123-144.dsl.chcgil.sbcglobal.net. [99.141.123.144]) 21, 39 -- by mx.google.com with ESMTPSA id kt2sm1024702igb.1.2014.01.15.19.26.13 21, 39 -- for {microscopy-at-microscopy.com} 21, 39 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 21, 39 -- Wed, 15 Jan 2014 19:26:16 -0800 (PST) 21, 39 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 21, 39 -- Message-ID: {52D75147.5040201-at-microscopy.com} 21, 39 -- Date: Wed, 15 Jan 2014 21:25:59 -0600 21, 39 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 21, 39 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 39 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 21, 39 -- MIME-Version: 1.0 21, 39 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 39 -- Subject: viaWWW:SCSMM full-day symposium February 8th 2014 21, 39 -- References: {201401160106.s0G163B9017479-at-ns.microscopy.com} 21, 39 -- In-Reply-To: {201401160106.s0G163B9017479-at-ns.microscopy.com} 21, 39 -- X-Forwarded-Message-Id: {201401160106.s0G163B9017479-at-ns.microscopy.com} 21, 39 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 39 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We are keeping our XP machine unplugged from the network, and I made sure to turn off "autorun" for all media (CD/DVD and USB) so infected media can't take over your machine automatically (unless there's a new way to get Windows to automatically execute files, which wouldn't surprise me).
Cheers,
Nick Botto Microprobe Specialist UC Davis Earth and Planetary Sciences Microprobe Lab Website: http://microprobe.geology.ucdavis.edu/index.html Calendar: http://microprobe.geology.ucdavis.edu/calendar/probe.htm ph. 530-752-6582
On 1/15/14, 1:21 PM, John.Mardinly-at-asu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } April 8, 2014 marks the end of support from Microsoft for Windows XP. That means no more security updates to patch newly discovered security holes. This will leave any computers still running Windows XP vulnerable to attacks if they remain connected to the internet or if infected memory media are inserted into the computer. I am sure there are a lot of machines out there running Windows XP. I am curious to hear what approaches microscopy community members are taking to deal with this, as well as what the community of microscopy related hardware vendors are doing to address this situation. Thank you. } } John Mardinly, ASU } } } } ==============================Original Headers============================== } 4, 35 -- From John.Mardinly-at-asu.edu Wed Jan 15 15:12:14 2014 } 4, 35 -- Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) } 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0FLCEU1008827 } 4, 35 -- for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 15:12:14 -0600 } 4, 35 -- X-ASG-Debug-ID: 1389820333-0549c456250a750001-FOsErg } 4, 35 -- Received: from exhubw01.asurite.ad.asu.edu (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnetw3.asu.edu with ESMTP id Lezl3NXq5dB4vpTh (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 14:12:13 -0700 (MST) } 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 } 4, 35 -- X-ASG-Whitelist: Client } 4, 35 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by } 4, 35 -- exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id 14.02.0309.002; } 4, 35 -- Wed, 15 Jan 2014 14:12:12 -0700 } 4, 35 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 4, 35 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} } 4, 35 -- Subject: Windows XP Support Ending April 8, 2014 } 4, 35 -- Thread-Topic: Windows XP Support Ending April 8, 2014 } 4, 35 -- X-ASG-Orig-Subj: Windows XP Support Ending April 8, 2014 } 4, 35 -- Thread-Index: Ac8SNnTksb2ozlmjTzO6+BJCz2aDAQ== } 4, 35 -- Date: Wed, 15 Jan 2014 21:12:11 +0000 } 4, 35 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400B7A2850-at-exmbt05.asurite.ad.asu.edu} } 4, 35 -- Accept-Language: en-US } 4, 35 -- Content-Language: en-US } 4, 35 -- X-MS-Has-Attach: } 4, 35 -- X-MS-TNEF-Correlator: } 4, 35 -- x-originating-ip: [129.219.103.4] } 4, 35 -- Content-Type: text/plain; charset="us-ascii" } 4, 35 -- MIME-Version: 1.0 } 4, 35 -- X-Barracuda-Connect: exhubw01.asurite.ad.asu.edu[129.219.4.199] } 4, 35 -- X-Barracuda-Start-Time: 1389820333 } 4, 35 -- X-Barracuda-Encrypted: AES128-SHA } 4, 35 -- X-Barracuda-URL: http://149.169.2.73:8000/cgi-mod/mark.cgi } 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu } 4, 35 -- X-Barracuda-BRTS-Status: 1 } 4, 35 -- Content-Transfer-Encoding: 8bit } 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0FLCEU1008827 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 23 -- From nwbotto-at-ucdavis.edu Thu Jan 16 12:13:31 2014 5, 23 -- Received: from msa1.ucdavis.edu (msa1.ucdavis.edu [128.120.32.181]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0GIDUKG029552 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 12:13:30 -0600 5, 23 -- Received: from distortedreality.local ([169.237.180.55]) 5, 23 -- (authenticated bits=0) 5, 23 -- by msa1.ucdavis.edu (8.14.4/8.14.5/it-rhel5-mimedefang-smtp-1.6) with ESMTP id s0GIDNGU061882 5, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-CAMELLIA256-SHA bits=256 verify=NO) 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 10:13:24 -0800 5, 23 -- Message-ID: {52D82143.9030301-at-ucdavis.edu} 5, 23 -- Date: Thu, 16 Jan 2014 10:13:23 -0800 5, 23 -- From: Nick Botto {nwbotto-at-ucdavis.edu} 5, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 5, 23 -- MIME-Version: 1.0 5, 23 -- To: microscopy-at-microscopy.com 5, 23 -- Subject: Re: [Microscopy] Windows XP Support Ending April 8, 2014 5, 23 -- References: {201401152121.s0FLLWhR023627-at-ns.microscopy.com} 5, 23 -- In-Reply-To: {201401152121.s0FLLWhR023627-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-Virus-Scanned: clamav-milter 0.97.8 at msa1 5, 23 -- X-Virus-Status: Clean 5, 23 -- X-Scanned-By: MIMEDefang 2.74 ==============================End of - Headers==============================
As an addendum to this, Microsoft announced on January 15th that it would continue to provide updates for security products until July 14, 2015. So no more system updates, but at least they'll continue to update Security Essentials and other MS antivirus signatures for machines running XP.
-- Adam Schuetze, D.Tech, B.Eng Laboratory Engineer, Advanced Microscopy Facility Bob Wright A015, University of Victoria, Canada adamschu-at-uvic.ca Lab: 250.853.3968 http://www.stehm.uvic.ca
-----Original Message----- X-from: nwbotto-at-ucdavis.edu [mailto:nwbotto-at-ucdavis.edu] Sent: Thursday, January 16, 2014 10:28 AM To: Adam Schuetze
We are keeping our XP machine unplugged from the network, and I made sure to turn off "autorun" for all media (CD/DVD and USB) so infected media can't take over your machine automatically (unless there's a new way to get Windows to automatically execute files, which wouldn't surprise me).
Cheers,
Nick Botto Microprobe Specialist UC Davis Earth and Planetary Sciences Microprobe Lab Website: http://microprobe.geology.ucdavis.edu/index.html Calendar: http://microprobe.geology.ucdavis.edu/calendar/probe.htm ph. 530-752-6582
On 1/15/14, 1:21 PM, John.Mardinly-at-asu.edu wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } April 8, 2014 marks the end of support from Microsoft for Windows XP. That means no more security updates to patch newly discovered security holes. This will leave any computers still running Windows XP vulnerable to attacks if they remain connected to the internet or if infected memory media are inserted into the computer. I am sure there are a lot of machines out there running Windows XP. I am curious to hear what approaches microscopy community members are taking to deal with this, as well as what the community of microscopy related hardware vendors are doing to address this situation. Thank you. } } John Mardinly, ASU } } } } ==============================Original } Headers============================== } 4, 35 -- From John.Mardinly-at-asu.edu Wed Jan 15 15:12:14 2014 4, 35 -- } Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) } 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0FLCEU1008827 } 4, 35 -- for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 15:12:14 -0600 } 4, 35 -- X-ASG-Debug-ID: 1389820333-0549c456250a750001-FOsErg } 4, 35 -- Received: from exhubw01.asurite.ad.asu.edu } (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnetw3.asu.edu with } ESMTP id Lezl3NXq5dB4vpTh (version=TLSv1 cipher=AES128-SHA bits=128 } verify=NO) for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 14:12:13 } -0700 (MST) 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 4, 35 -- } X-ASG-Whitelist: Client 4, 35 -- Received: from } EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 4, 35 -- } exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id } 14.02.0309.002; 4, 35 -- Wed, 15 Jan 2014 14:12:12 -0700 4, 35 -- } From: John Mardinly {John.Mardinly-at-asu.edu} 4, 35 -- To: } "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 4, 35 -- } Subject: Windows XP Support Ending April 8, 2014 4, 35 -- } Thread-Topic: Windows XP Support Ending April 8, 2014 4, 35 -- } X-ASG-Orig-Subj: Windows XP Support Ending April 8, 2014 4, 35 -- } Thread-Index: Ac8SNnTksb2ozlmjTzO6+BJCz2aDAQ== 4, 35 -- Date: Wed, 15 } Jan 2014 21:12:11 +0000 4, 35 -- Message-ID: } {4FFC63ED830B1C41BACD3ABA182BE9400B7A2850-at-exmbt05.asurite.ad.asu.edu} } 4, 35 -- Accept-Language: en-US } 4, 35 -- Content-Language: en-US } 4, 35 -- X-MS-Has-Attach: } 4, 35 -- X-MS-TNEF-Correlator: } 4, 35 -- x-originating-ip: [129.219.103.4] 4, 35 -- Content-Type: } text/plain; charset="us-ascii" } 4, 35 -- MIME-Version: 1.0 } 4, 35 -- X-Barracuda-Connect: } exhubw01.asurite.ad.asu.edu[129.219.4.199] } 4, 35 -- X-Barracuda-Start-Time: 1389820333 4, 35 -- } X-Barracuda-Encrypted: AES128-SHA 4, 35 -- X-Barracuda-URL: } http://149.169.2.73:8000/cgi-mod/mark.cgi } 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 35 -- } X-Barracuda-BRTS-Status: 1 4, 35 -- Content-Transfer-Encoding: 8bit 4, } 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s0FLCEU1008827 ==============================End } of - Headers============================== }
==============================Original Headers============================== 5, 23 -- From nwbotto-at-ucdavis.edu Thu Jan 16 12:13:31 2014 5, 23 -- Received: from msa1.ucdavis.edu (msa1.ucdavis.edu [128.120.32.181]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0GIDUKG029552 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 12:13:30 -0600 5, 23 -- Received: from distortedreality.local ([169.237.180.55]) 5, 23 -- (authenticated bits=0) 5, 23 -- by msa1.ucdavis.edu (8.14.4/8.14.5/it-rhel5-mimedefang-smtp-1.6) with ESMTP id s0GIDNGU061882 5, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-CAMELLIA256-SHA bits=256 verify=NO) 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 10:13:24 -0800 5, 23 -- Message-ID: {52D82143.9030301-at-ucdavis.edu} 5, 23 -- Date: Thu, 16 Jan 2014 10:13:23 -0800 5, 23 -- From: Nick Botto {nwbotto-at-ucdavis.edu} 5, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 5, 23 -- MIME-Version: 1.0 5, 23 -- To: microscopy-at-microscopy.com 5, 23 -- Subject: Re: [Microscopy] Windows XP Support Ending April 8, 2014 5, 23 -- References: {201401152121.s0FLLWhR023627-at-ns.microscopy.com} 5, 23 -- In-Reply-To: {201401152121.s0FLLWhR023627-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-Virus-Scanned: clamav-milter 0.97.8 at msa1 5, 23 -- X-Virus-Status: Clean 5, 23 -- X-Scanned-By: MIMEDefang 2.74 ==============================End of - Headers==============================
==============================Original Headers============================== 14, 31 -- From adamschu-at-uvic.ca Thu Jan 16 13:31:21 2014 14, 31 -- Received: from mole.comp.uvic.ca (mole.comp.uvic.ca [142.104.5.70]) 14, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0GJVKdY020853 14, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Jan 2014 13:31:21 -0600 14, 31 -- Received: from LONGFIN.uvic.ca (longfin.uvic.ca [142.104.240.132]) 14, 31 -- by mole.comp.uvic.ca (8.14.4/8.14.4) with ESMTP id s0GJVJWO018632 14, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Jan 2014 11:31:19 -0800 14, 31 -- Received: from OPAH.uvic.ca ([fe80::ac3a:7aab:abe5:2938]) by LONGFIN.uvic.ca 14, 31 -- ([fe80::200:5efe:142.104.240.132%11]) with mapi id 14.03.0174.001; Thu, 16 14, 31 -- Jan 2014 11:31:19 -0800 14, 31 -- From: Adam Schuetze {adamschu-at-uvic.ca} 14, 31 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 14, 31 -- Subject: RE: [Microscopy] Re: Windows XP Support Ending April 8, 2014 14, 31 -- Thread-Topic: [Microscopy] Re: Windows XP Support Ending April 8, 2014 14, 31 -- Thread-Index: AQHPEui/YjZpFKYJTkyeyiurYTnuz5qHu/qg 14, 31 -- Date: Thu, 16 Jan 2014 19:31:18 +0000 14, 31 -- Message-ID: {3F1048D9CBC6AA4092B13141A4B0F44C1590B571-at-opah.uvic.ca} 14, 31 -- References: {201401161828.s0GISOEJ015766-at-ns.microscopy.com} 14, 31 -- In-Reply-To: {201401161828.s0GISOEJ015766-at-ns.microscopy.com} 14, 31 -- Accept-Language: en-CA, en-US 14, 31 -- Content-Language: en-US 14, 31 -- X-MS-Has-Attach: 14, 31 -- X-MS-TNEF-Correlator: 14, 31 -- x-originating-ip: [142.104.193.193] 14, 31 -- Content-Type: text/plain; charset="us-ascii" 14, 31 -- MIME-Version: 1.0 14, 31 -- X-UVic-Virus-Scanned: OK - Passed virus scan by ClamAV (clamd) on mole 14, 31 -- X-UVic-Scan: mole.comp.uvic.ca filter_version 3.7.6 14, 31 -- X-Scanned-By: MIMEDefang 2.67 on 142.104.5.77 14, 31 -- Content-Transfer-Encoding: 8bit 14, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0GJVKdY020853 ==============================End of - Headers==============================
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Message: Does anyone know what the heat load that the CM10 and CM12 generate?
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 16 17:53:48 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0GNrmsa018377 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 17:53:48 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id 591A86A9D58 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 17:53:48 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id wKvCqzg1f-XA for {microscopy-at-microscopy.com} ; 12, 28 -- Thu, 16 Jan 2014 17:53:43 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id CB2956A9D46 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 17:53:43 -0600 (CST) 12, 28 -- Message-ID: {52D87107.3070502-at-microscopy.com} 12, 28 -- Date: Thu, 16 Jan 2014 17:53:43 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:CM10/12 heat production 12, 28 -- References: {201401161941.s0GJfnim006532-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201401161941.s0GJfnim006532-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201401161941.s0GJfnim006532-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I've got the heat load specs for the CM-10 - just have to dig them out. I'll send you a pdf. Not for the CM-12, but they won't be too different.
Phil
} Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10/12 heat production } } Message: Does anyone know what the heat load that the CM10 and CM12 generate? } -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 29 -- From oshel1pe-at-cmich.edu Fri Jan 17 07:06:44 2014 4, 29 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0HD6haQ005955 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Jan 2014 07:06:44 -0600 4, 29 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75] (may be forged)) 4, 29 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s0HCxcJZ004736; 4, 29 -- Fri, 17 Jan 2014 07:59:38 -0500 4, 29 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 4, 29 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 29 -- 14.3.123.3; Fri, 17 Jan 2014 08:06:41 -0500 4, 29 -- Message-ID: {52D92ADC.1040402-at-cmich.edu} 4, 29 -- Date: Fri, 17 Jan 2014 08:06:36 -0500 4, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 29 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 29 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 29 -- MIME-Version: 1.0 4, 29 -- To: {jcsmtf-at-mail.missouri.edu} , micro {Microscopy-at-microscopy.com} 4, 29 -- Subject: Re: [Microscopy] viaWWW:CM10/12 heat production 4, 29 -- References: {201401170008.s0H088tc003794-at-ns.microscopy.com} 4, 29 -- In-Reply-To: {201401170008.s0H088tc003794-at-ns.microscopy.com} 4, 29 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 29 -- Content-Transfer-Encoding: 7bit 4, 29 -- X-Originating-IP: [141.209.160.122] 4, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 29 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 29 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 29 -- X-CanItPRO-Stream: default 4, 29 -- X-Canit-Stats-ID: 05Lf0XCzD - 43de1d4dd168 - 20140117 4, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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Email: sliliensiek-at-ibidiusa.com Name: Sara Liliensiek
Organization: ibidi, LLC
Title-Subject: [Filtered] ibidi, LLC is Now Hiring for a Techincal Sales Representative
Message: Hello Everyone,
ibidi, LLC, a leader in providing solutions for BioMicroscopy, is looking to add a new Technical Sales Representative to the US team. ibidi is an international company which began in Munich Germany and has now expanded in the US. We are looking for a motivated and technical individual to help grow and manage new territories in the East and/or West Coast market. This position is geared towards someone who is dedicated to life sciences and cellular research but is more suited to work in the field rather than the lab.
The successful candidate will be a self-starter with strong communication skills as well as a forward thinker. This is an amazing opportunity to work for an innovative and fast growing company and the correct individual will be provided the tools and support to advance quickly.
If interested, please send your Cover Letter, CV along with salary requirements to Jenni Poole at jpoole-at-ibidi.com.
For a more detailed job description please go to http://ibidi.com/about-ibidi/career/technical-sales-representative/
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 17 09:17:10 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0HFHAPv029038 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jan 2014 09:17:10 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id EFF836AA4B0 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jan 2014 09:17:09 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id enULFILziQBT for {microscopy-at-microscopy.com} ; 19, 28 -- Fri, 17 Jan 2014 09:17:08 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 8DA6A6AA4A3 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jan 2014 09:17:08 -0600 (CST) 19, 28 -- Message-ID: {52D94974.3010404-at-microscopy.com} 19, 28 -- Date: Fri, 17 Jan 2014 09:17:08 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:ibidi, LLC is Now Hiring for a Techincal Sales Representative 19, 28 -- References: {201401171404.s0HE4Yf0025912-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201401171404.s0HE4Yf0025912-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201401171404.s0HE4Yf0025912-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: mpittman-at-hku.hk Name: Michael Pittman
Organization: Department of Earth Sciences, The University of Hong Kong
Title-Subject: [Filtered] Stereoscope with a camera mount for studying dinosaur fossils
Message: Hi there,
My name is Michael Pittman and I am a British Research Assistant Professor investigating the evolution of carnivorous dinosaurs at the University of Hong Kong.
I am looking to purchase my first stereomicroscope for my work and I am a novice towards the science of microscopy.
I have a list of things I am looking for in my setup having asked some colleagues for advice and having looked into the models I used previously. If anyone has or knows of anyone who has equipment for sale that can meet most of the requirements below it would be really great to hear from you.
-Leica, Wild or Olympus brand -~x4 to x100 total magnification range -Camera mount for a DSLR -UV illumination port -Large boom stand for sliding vertically and horizontally over my specimens (these are usually on rock slabs up to 3ft squared) -ring light -A maximum budget of USD$6500-7000 (my understanding is that I would probably only be able to afford a second-hand microscope which is totally fine)
Thanks very much for considering my request.
Best regards, Michael
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Title-Subject: [Filtered] Electron Microscopist Position Available - The Rockefeller University
Message: Title-Subject: [Filtered] Electron Microscopist Position Available
Message: Electron Microscopist, The Rockefeller University, NY, NY
The Rockefeller University, a premier biomedical research institution, seeks a Research Support Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state of the art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a free substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Specialist will participate in all of the Electron Microscopy Resource Center's (EMRC) daily operations, including bench work in preparation for transmission and scanning electron microscopy and maintenance of the center. Will be responsible for all parts of sample preparation, including cutting ultrathin sections, maintenance and operation of EM and other equipment, training users, consulting with scientists on design of experiments, processing/analyzing data and interpretation of results, ordering and receiving supplies, managing chemical waste compliance, administrative support for office duties and center billing, and keeping up with the latest methodology via familiarity with the literature. Ph.D. in biology, cell biology, bioengineering or a related field required. Must have a minimum of 5 years of hands-on experience in electron microscopy and have strong communication skills, and the ability to work collaboratively on a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.
Our culture *inspiring, collaborative atmosphere *strong social and environmental consciousness *intellectually curious academic environment *14-acre campus setting in NYC
We offer a competitive salary, comprehensive benefits, and a beautiful work environment.
To apply to this job, click the following URL, click on 'staff opportunities’ and enter keyword ‘IRC14819’: http://www.rockefeller.edu/hr/career.php
The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Kunihiro Uryu, Ph.D., Director of Electron Microscopy Resource Center (EMRC) The Rockefeller University 1230 York Ave., Box 230 New York, NY 10065
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Email: marzena_sujkowska-at-sggw.pl Name: Marzena
Organization: Warsaw University of Life Sciences, Poland
Title-Subject: [Filtered] Mycorrhiza
Message:
How stain the roots with mycorrhiza for arbuscules before maunting in EPON resin for TEM microscopy?
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 21 07:00:05 2014 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0LD05xS025728 15, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 07:00:05 -0600 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id 6F50E6ACC4C 15, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 07:00:05 -0600 (CST) 15, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id IgYVdtoDsvY8 for {microscopy-at-microscopy.com} ; 15, 28 -- Tue, 21 Jan 2014 07:00:01 -0600 (CST) 15, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 19D806ACC3A 15, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 07:00:01 -0600 (CST) 15, 28 -- Message-ID: {52DE6F50.8010802-at-microscopy.com} 15, 28 -- Date: Tue, 21 Jan 2014 07:00:00 -0600 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:stain the roots with mycorrhiza 15, 28 -- References: {201401210928.s0L9ShlX022320-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201401210928.s0L9ShlX022320-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201401210928.s0L9ShlX022320-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
we find strange bubbles when embedding Bacillus subtilis bacteria in LR white resin. The bubbles prevent the resin from polymerising, does anybody have an idea how and why this could happen?
These bubbles seem to be trapped air in the resin. Put the specimens in the final resin (in the embedding capsules) and left them for an hour. The air bubbles will come to the surface and you can easily break them with a needle. Then put the capsules to the oven for polymerisation.
Panos
Quoting stefan.wernitznig-at-medunigraz.at:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } we find strange bubbles when embedding Bacillus subtilis bacteria in LR } white resin. The bubbles prevent the resin from polymerising, does } anybody have an idea how and why this could happen? } } http://chimerism.medunigraz.at/Pictures/pioloform01.php } http://chimerism.medunigraz.at/Pictures/pioloform02.php } } Many thanks, } Stefan } } } ==============================Original Headers============================== } 5, 30 -- From stefan.wernitznig-at-medunigraz.at Tue Jan 21 09:30:56 2014 } 5, 30 -- Received: from si069.medunigraz.at (si082.medunigraz.at } [193.170.105.82]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s0LFUt90018127 } 5, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 09:30:56 -0600 } 5, 30 -- X-AuditID: 0ac80c45-f79db6d000001194-e2-52de92ae462d } 5, 30 -- Received: from si063.medunigraz.at ( [10.200.18.79]) } 5, 30 -- by si069.medunigraz.at (Symantec Mail Security) with SMTP } id 51.2C.04500.EA29ED25; Tue, 21 Jan 2014 16:30:54 +0100 (CET) } 5, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at } [10.200.112.128]) } 5, 30 -- by si063.medunigraz.at with ESMTP (TLS encrypted); Tue, 21 } Jan 2014 16:30:47 +0100 } 5, 30 -- Message-ID: {52DE92A9.9030803-at-medunigraz.at} } 5, 30 -- Date: Tue, 21 Jan 2014 16:30:49 +0100 } 5, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} } 5, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) } Gecko/20130801 Thunderbird/17.0.8 } 5, 30 -- MIME-Version: 1.0 } 5, 30 -- To: Microscopy-at-microscopy.com } 5, 30 -- Subject: Strange bubbles in LR white resin } 5, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 5, 30 -- Content-Transfer-Encoding: 7bit } 5, 30 -- X-Brightmail-Tracker: } H4sIAAAAAAAAA+NgFtrGIsWRmVeSWpSXmKPExsXCdULIX3fdpHtBBvsalSz+HG5gd2D0+Nj9 } 5, 30 -- } mimAMYrLJiU1J7MstUjfLoErY2uTQ8FTpoq5zyeyNDCuZupi5OSQEDCReNTylgXCFpO4cG89 } 5, 30 -- } WxcjF4eQwHpGiW//97NDOPMYJTZvnsXaxcjBwSugIzGrMRikgUVAVeLltjNsIDabgKXE8e4f } 5, 30 -- } YINEBYIldj9+xgpi8woISpyc+QQsLiIgK7Hg/RF2EFtYQEuiYfNiMJtZwEbi74lFLBC2vMT2 } 5, 30 -- } t3OYJzDyzkLSPgtJ2SwkZQsYmVcxChdnGphZ6uWmppTmZaYXJVbpJZZsYgQHDY/rDsZdTcKH } 5, 30 -- } GAU4GJV4eB913AsSYk0sK67MPcQoycGkJMobMxEoxJeUn1KZkVicEV9UmpNafIhRgoNZSYS3 } 5, 30 -- } bAJQjjclsbIqtSgfJiXNwaIkzrtF4m6QkEB6YklqdmpqQWoRTFaGg0NJgrcOZKhgUWp6akVa } 5, 30 -- } Zk4JQpqJgxNkOA/Q8DkgNbzFBYm5xZnpEPlTjKoc+1Z8+sMoxJKXn5cqJc77BeQCAZCijNI8 } 5, 30 -- } uDmvGMWB3hHmLQYZwQNMDHATXgENZwIafvvSHZDhJYkIKakGxjDeyX5bznE99pK4zPFK7ToP } 5, 30 -- } 75HXXFcf3Hnw6ul1yUfzBT7t43Rj5fokYNR71qhGLlaaN2yH+xbfw1Lfrx3M4zlZ+8T3oZ+P } 5, 30 -- } nt4MS7177Bf1ZgTOef442XLH930Wd16uT71mvHkd8/a+W2yla0P4y32Y1+5rucm7qic++emH } 5, 30 -- y49nsdS7fjth16bEUpyRaKjFXFScCABGPUGSyQIAAA== } ==============================End of - Headers==============================
-- Dr. Berillis Panagiotis Lecturer in Microscopy, image analysis and histology Department of Ichthyology and Aquatic Environment Agricultural school University of Thessaly
==============================Original Headers============================== 8, 21 -- From pveril-at-apae.uth.gr Tue Jan 21 13:53:04 2014 8, 21 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0LJr3KI015109 8, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 13:53:04 -0600 8, 21 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) 8, 21 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0LJreSM013826; 8, 21 -- Tue, 21 Jan 2014 21:53:40 +0200 8, 21 -- Received: from adsl-213.91.140.88.tellas.gr (adsl-213.91.140.88.tellas.gr 8, 21 -- [91.140.88.213]) by webmail.uth.gr (Horde Framework) with HTTP; Tue, 21 Jan 8, 21 -- 2014 21:52:52 +0200 8, 21 -- Date: Tue, 21 Jan 2014 21:52:52 +0200 8, 21 -- Message-ID: {20140121215252.Horde.5K4CvlZsTmoxhFGI1v_E0w1-at-webmail.uth.gr} 8, 21 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} 8, 21 -- To: stefan.wernitznig-at-medunigraz.at, Microscopy-at-microscopy.com 8, 21 -- Subject: Re: [Microscopy] Strange bubbles in LR white resin 8, 21 -- References: {201401211539.s0LFds8O029163-at-ns.microscopy.com} 8, 21 -- In-Reply-To: {201401211539.s0LFds8O029163-at-ns.microscopy.com} 8, 21 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) 8, 21 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Disposition: inline ==============================End of - Headers==============================
Those don't look like air bubbles to me. They look to being something extracted from the bacteria. Maybe not fully dehydrated? Some non-miscible solvent still around? Air bubbles shouldn't prevent LRW from polymerizing. Does a "blank" block with no cells polymerize?
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: pveril-at-apae.uth.gr [mailto:pveril-at-apae.uth.gr] Sent: Tuesday, January 21, 2014 1:54 PM To: Phillips, Thomas E.
Dear Stefan
These bubbles seem to be trapped air in the resin. Put the specimens in the final resin (in the embedding capsules) and left them for an hour. The air bubbles will come to the surface and you can easily break them with a needle. Then put the capsules to the oven for polymerisation.
Panos
Quoting stefan.wernitznig-at-medunigraz.at:
} ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Dear Listers, } } we find strange bubbles when embedding Bacillus subtilis bacteria in } LR white resin. The bubbles prevent the resin from polymerising, does } anybody have an idea how and why this could happen? } } http://chimerism.medunigraz.at/Pictures/pioloform01.php } http://chimerism.medunigraz.at/Pictures/pioloform02.php } } Many thanks, } Stefan } } } ==============================Original } Headers============================== } 5, 30 -- From stefan.wernitznig-at-medunigraz.at Tue Jan 21 09:30:56 2014 } 5, 30 -- Received: from si069.medunigraz.at (si082.medunigraz.at } [193.170.105.82]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s0LFUt90018127 } 5, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 09:30:56 -0600 } 5, 30 -- X-AuditID: 0ac80c45-f79db6d000001194-e2-52de92ae462d } 5, 30 -- Received: from si063.medunigraz.at ( [10.200.18.79]) } 5, 30 -- by si069.medunigraz.at (Symantec Mail Security) with SMTP } id 51.2C.04500.EA29ED25; Tue, 21 Jan 2014 16:30:54 +0100 (CET) 5, 30 } -- Received: from [10.200.112.128] (dc112128.medunigraz.at } [10.200.112.128]) } 5, 30 -- by si063.medunigraz.at with ESMTP (TLS encrypted); Tue, 21 } Jan 2014 16:30:47 +0100 } 5, 30 -- Message-ID: {52DE92A9.9030803-at-medunigraz.at} 5, 30 -- Date: } Tue, 21 Jan 2014 16:30:49 +0100 5, 30 -- From: Stefan Wernitznig } {stefan.wernitznig-at-medunigraz.at} 5, 30 -- User-Agent: Mozilla/5.0 } (Windows NT 5.1; rv:17.0) } Gecko/20130801 Thunderbird/17.0.8 } 5, 30 -- MIME-Version: 1.0 } 5, 30 -- To: Microscopy-at-microscopy.com 5, 30 -- Subject: Strange } bubbles in LR white resin 5, 30 -- Content-Type: text/plain; } charset=ISO-8859-15; format=flowed 5, 30 -- Content-Transfer-Encoding: } 7bit 5, 30 -- X-Brightmail-Tracker: } H4sIAAAAAAAAA+NgFtrGIsWRmVeSWpSXmKPExsXCdULIX3fdpHtBBvsalSz+HG5gd2D0+N } H4sIAAAAAAAAA+NgFtrGIsWRmVeSWpSXmKPExsXCdULIX3fdpHtBBvsalSz+HG5gd2D0+j } H4sIAAAAAAAAA+NgFtrGIsWRmVeSWpSXmKPExsXCdULIX3fdpHtBBvsalSz+HG5gd2D0+9 } 5, 30 -- } } mimAMYrLJiU1J7MstUjfLoErY2uTQ8FTpoq5zyeyNDCuZupi5OSQEDCReNTylgXCFpO4cG } 89 } 5, 30 -- } } WxcjF4eQwHpGiW//97NDOPMYJTZvnsXaxcjBwSugIzGrMRikgUVAVeLltjNsIDabgKXE8e } 4f } 5, 30 -- } } YINEBYIldj9+xgpi8woISpyc+QQsLiIgK7Hg/RF2EFtYQEuiYfNiMJtZwEbi74lFLBC2vM } T2 } 5, 30 -- } } t3OYJzDyzkLSPgtJ2SwkZQsYmVcxChdnGphZ6uWmppTmZaYXJVbpJZZsYgQHDY/rDsZdTc } KH } 5, 30 -- } } GAU4GJV4eB913AsSYk0sK67MPcQoycGkJMobMxEoxJeUn1KZkVicEV9UmpNafIhRgoNZSY } S3 } 5, 30 -- } } bAJQjjclsbIqtSgfJiXNwaIkzrtF4m6QkEB6YklqdmpqQWoRTFaGg0NJgrcOZKhgUWp6ak } Va } 5, 30 -- } } Zk4JQpqJgxNkOA/Q8DkgNbzFBYm5xZnpEPlTjKoc+1Z8+sMoxJKXn5cqJc77BeQCAZCijN } I8 } 5, 30 -- } } uDmvGMWB3hHmLQYZwQNMDHATXgENZwIafvvSHZDhJYkIKakGxjDeyX5bznE99pK4zPFK7T } oP } 5, 30 -- } } 75HXXFcf3Hnw6ul1yUfzBT7t43Rj5fokYNR71qhGLlaaN2yH+xbfw1Lfrx3M4zlZ+8T3oZ } +P } 5, 30 -- } nt4MS7177Bf1ZgTOef442XLH930Wd16uT71mvHkd8/a+W2yla0P4y32Y1+5rucm7qic++emH } 5, 30 -- y49nsdS7fjth16bEUpyRaKjFXFScCABGPUGSyQIAAA== } ==============================End of - } Headers==============================
-- Dr. Berillis Panagiotis Lecturer in Microscopy, image analysis and histology Department of Ichthyology and Aquatic Environment Agricultural school University of Thessaly
==============================Original Headers============================== 8, 21 -- From pveril-at-apae.uth.gr Tue Jan 21 13:53:04 2014 8, 21 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0LJr3KI015109 8, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 13:53:04 -0600 8, 21 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) 8, 21 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0LJreSM013826; 8, 21 -- Tue, 21 Jan 2014 21:53:40 +0200 8, 21 -- Received: from adsl-213.91.140.88.tellas.gr (adsl-213.91.140.88.tellas.gr 8, 21 -- [91.140.88.213]) by webmail.uth.gr (Horde Framework) with HTTP; Tue, 21 Jan 8, 21 -- 2014 21:52:52 +0200 8, 21 -- Date: Tue, 21 Jan 2014 21:52:52 +0200 8, 21 -- Message-ID: {20140121215252.Horde.5K4CvlZsTmoxhFGI1v_E0w1-at-webmail.uth.gr} 8, 21 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} 8, 21 -- To: stefan.wernitznig-at-medunigraz.at, Microscopy-at-microscopy.com 8, 21 -- Subject: Re: [Microscopy] Strange bubbles in LR white resin 8, 21 -- References: {201401211539.s0LFds8O029163-at-ns.microscopy.com} 8, 21 -- In-Reply-To: {201401211539.s0LFds8O029163-at-ns.microscopy.com} 8, 21 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) 8, 21 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 18, 30 -- From PhillipsT-at-missouri.edu Tue Jan 21 14:35:21 2014 18, 30 -- Received: from um-tip1-missouri-out.um.umsystem.edu (um-tip1-missouri-out.um.umsystem.edu [198.209.49.135]) 18, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0LKZLsQ004021 18, 30 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 14:35:21 -0600 18, 30 -- X-IronPort-Anti-Spam-Filtered: true 18, 30 -- X-IronPort-Anti-Spam-Result: AhgFAMXZ3lLPoJ7E/2dsb2JhbAA/FgEDgws4VqlskgaBFhZ0giUBAQEEOhEBAjcCAgIBCBEDAQEBCwISCQcWGwEUAwEFCAIECgkIFQSHZA02qwwBkkCEfxcEjHANA4EqAQERDQYbEgUGC4IedYEUBJlUiySFQoMtgXE5 18, 30 -- X-IPAS-Result: AhgFAMXZ3lLPoJ7E/2dsb2JhbAA/FgEDgws4VqlskgaBFhZ0giUBAQEEOhEBAjcCAgIBCBEDAQEBCwISCQcWGwEUAwEFCAIECgkIFQSHZA02qwwBkkCEfxcEjHANA4EqAQERDQYbEgUGC4IedYEUBJlUiySFQoMtgXE5 18, 30 -- Received: from um-tcas1.um.umsystem.edu ([207.160.158.196]) 18, 30 -- by um-tip1-exch-relay.um.umsystem.edu with ESMTP; 21 Jan 2014 14:35:19 -0600 18, 30 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.204]) by 18, 30 -- UM-TCAS1.um.umsystem.edu ([207.160.158.196]) with mapi id 14.03.0169.001; 18, 30 -- Tue, 21 Jan 2014 14:35:19 -0600 18, 30 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 18, 30 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 18, 30 -- Subject: RE: [Microscopy] Re: Strange bubbles in LR white resin 18, 30 -- Thread-Topic: [Microscopy] Re: Strange bubbles in LR white resin 18, 30 -- Thread-Index: AQHPFuKL/s5jc3XgnkO+lpcDg5FRmpqPojAA 18, 30 -- Date: Tue, 21 Jan 2014 20:35:18 +0000 18, 30 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8E7178D-at-UM-MBX-T02.um.umsystem.edu} 18, 30 -- References: {201401211954.s0LJs5Ds016021-at-ns.microscopy.com} 18, 30 -- In-Reply-To: {201401211954.s0LJs5Ds016021-at-ns.microscopy.com} 18, 30 -- Accept-Language: en-US 18, 30 -- Content-Language: en-US 18, 30 -- X-MS-Has-Attach: 18, 30 -- X-MS-TNEF-Correlator: 18, 30 -- x-originating-ip: [128.206.81.115] 18, 30 -- Content-Type: text/plain; charset="us-ascii" 18, 30 -- MIME-Version: 1.0 18, 30 -- Content-Transfer-Encoding: 8bit 18, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0LKZLsQ004021 ==============================End of - Headers==============================
By chance did you happen to post fix the bacteria in osmium tetroxide? LR White does not polymerize well when osmium is used in the processing protocol.
Mary Ard Electron Microscopy Lab Coordinator Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, GA 30602 706-542-5537
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Email: paulwebsterphd-at-gmail.com Name: Paul Webster
Organization: University of Souther California
Title-Subject: [Filtered] LR White and air bubbles
Message: Dear Stephan,
I agree with Tom, that it looks as if something has been released from the bacteria. It could be lipid being extracted by the resin, which is a pretty good solvent.
I am not sure that this is the reason why the resin has not polymerized. From your images, it does look as if you have used osmium tetroxide to contrast the cells. It might also be possible that you also used acetone to dehydrate the cells.
Residual amounts of acetone may have interfered with resin polymerization in the way you describe. A more detailed description of your processing and embedding protocol may help diagnose the problem.
Paul Webster, University of Southern California Los Angeles, CA
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What is the average size of the "particles"? You do not have a size bar in your image to get a feel for their size, which is important in any viral identification. The "particles" do not appear to be associated with the sample on the grid but laying on top of the sample. In my opinion, they look more like crystalline structures rather than viral particles. Depending on their actual size, could they possibly be phosphotungstic acid crystals?
Kind regards, Mary
Mary Ard Electron Microscopy Lab Coordinator Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, GA 30602 706-542-5537
==============================Original Headers============================== 6, 29 -- From maryard-at-uga.edu Thu Jan 23 08:06:42 2014 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NE6gO0014516 6, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 08:06:42 -0600 6, 29 -- Received: from DM2PR02MB480.namprd02.prod.outlook.com (10.141.84.156) by 6, 29 -- DM2PR02MB477.namprd02.prod.outlook.com (10.141.84.143) with Microsoft SMTP 6, 29 -- Server (TLS) id 15.0.859.15; Thu, 23 Jan 2014 14:06:37 +0000 6, 29 -- Received: from DM2PR02MB480.namprd02.prod.outlook.com ([10.141.84.156]) by 6, 29 -- DM2PR02MB480.namprd02.prod.outlook.com ([10.141.84.156]) with mapi id 6, 29 -- 15.00.0859.013; Thu, 23 Jan 2014 14:06:37 +0000 6, 29 -- From: Mary Brown Ard {maryard-at-uga.edu} 6, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 29 -- Subject: [Microscopy] Virus 6, 29 -- Thread-Topic: [Microscopy] Virus 6, 29 -- Thread-Index: Ac8YQxrT3GMwtR30RFK9YaSHozxtFQ== 6, 29 -- Date: Thu, 23 Jan 2014 14:06:36 +0000 6, 29 -- Message-ID: {473dbc6ac41041b38ff74dd69ab35549-at-DM2PR02MB480.namprd02.prod.outlook.com} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- x-originating-ip: [128.192.89.8] 6, 29 -- x-forefront-prvs: 0100732B76 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(71364002)(189002)(199002)(4396001)(74502001)(47446002)(79102001)(83322001)(2656002)(81686001)(81816001)(65816001)(80022001)(75432001)(59766001)(50986001)(85306002)(47976001)(66066001)(81542001)(87266001)(77982001)(33646001)(47736001)(49866001)(87936001)(63696002)(31966008)(92566001)(74662001)(81342001)(80976001)(74876001)(76576001)(76176001)(76786001)(76796001)(69226001)(85852003)(83072002)(74316001)(93516002)(86362001)(90146001)(93136001)(56816005)(74366001)(51856001)(46102001)(74706001)(54316002)(56776001)(53806001)(76482001)(54356001)(94316002)(24736002);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR02MB477;H:DM2PR02MB480.namprd02.prod.outlook.com;CLIP:128.192.89.8;FPR:;InfoNoRecordsMX:1;A:1;LANG:en; 6, 29 -- Content-Type: text/plain; charset="us-ascii" 6, 29 -- MIME-Version: 1.0 6, 29 -- X-OriginatorOrg: uga.edu 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NE6gO0014516 ==============================End of - Headers==============================
Giving a picture without scale to a miscroscopist is like talking without words. We cannot make anything out if it! I doubt these objects are embedded in the resin. One can see a hole in the section and one object seems to partly cover it. Also they don't look like they were sectioned. Looks like artifacts for me.
Regards, Stephane
________________________________ X-from: "pveril-at-apae.uth.gr" {pveril-at-apae.uth.gr} To: nizets2-at-yahoo.com Sent: Thursday, January 23, 2014 11:29 AM
Dear all
During an electron microscope examination of a Sparus aurata liver collagen (PTA and uranyl acetate were used as stains) I came across to these objects. They are probably virus particles. Can any one identify them?
http://upload.users.uth.gr/files/virus.jpg
Many thanks Panos -- Dr. Berillis Panagiotis Lecturer in Microscopy, image analysis and histology Department of Ichthyology and Aquatic Environment Agricultural school University of Thessaly
==============================Original Headers============================== 6, 18 -- From pveril-at-apae.uth.gr Thu Jan 23 04:23:12 2014 6, 18 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NANBUa024847 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 04:23:12 -0600 6, 18 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) 6, 18 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0NANwCR031164 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 12:23:58 +0200 6, 18 -- Received: from 10.64.60.150 ([10.64.60.150]) by webmail.uth.gr (Horde 6, 18 -- Framework) with HTTP; Thu, 23 Jan 2014 12:23:00 +0200 6, 18 -- Date: Thu, 23 Jan 2014 12:23:00 +0200 6, 18 -- Message-ID: {20140123122300.Horde.--gLtdj-JhwWKO_juLlJ4w3-at-webmail.uth.gr} 6, 18 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: Virus 6, 18 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) 6, 18 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Disposition: inline ==============================End of - Headers==============================
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I agree, it does not looks like belonging to section. Some contamination after sectioning/staining. I have stained a lot with PTA, have never seen something like this.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, January 23, 2014 8:23 AM To: Dusevich, Vladimir
Hi Panos!
Giving a picture without scale to a miscroscopist is like talking without words. We cannot make anything out if it! I doubt these objects are embedded in the resin. One can see a hole in the section and one object seems to partly cover it. Also they don't look like they were sectioned. Looks like artifacts for me.
Regards, Stephane
________________________________ X-from: "pveril-at-apae.uth.gr" {pveril-at-apae.uth.gr} To: nizets2-at-yahoo.com Sent: Thursday, January 23, 2014 11:29 AM
Dear all
During an electron microscope examination of a Sparus aurata liver collagen (PTA and uranyl acetate were used as stains) I came across to these objects. They are probably virus particles. Can any one identify them?
http://upload.users.uth.gr/files/virus.jpg
Many thanks Panos -- Dr. Berillis Panagiotis Lecturer in Microscopy, image analysis and histology Department of Ichthyology and Aquatic Environment Agricultural school University of Thessaly
==============================Original Headers============================== 6, 18 -- From pveril-at-apae.uth.gr Thu Jan 23 04:23:12 2014 6, 18 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NANBUa024847 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 04:23:12 -0600 6, 18 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) 6, 18 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0NANwCR031164 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 12:23:58 +0200 6, 18 -- Received: from 10.64.60.150 ([10.64.60.150]) by webmail.uth.gr (Horde 6, 18 -- Framework) with HTTP; Thu, 23 Jan 2014 12:23:00 +0200 6, 18 -- Date: Thu, 23 Jan 2014 12:23:00 +0200 6, 18 -- Message-ID: {20140123122300.Horde.--gLtdj-JhwWKO_juLlJ4w3-at-webmail.uth.gr} 6, 18 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: Virus 6, 18 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) 6, 18 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Disposition: inline ==============================End of - Headers==============================
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==============================Original Headers============================== 25, 30 -- From DusevichV-at-umkc.edu Thu Jan 23 09:05:08 2014 25, 30 -- Received: from um-nip4-umkc-out.um.umsystem.edu (um-nip4-umkc-out.um.umsystem.edu [198.209.49.180]) 25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NF58WE020492 25, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 09:05:08 -0600 25, 30 -- X-IronPort-Anti-Spam-Filtered: true 25, 30 -- X-IronPort-Anti-Spam-Result: Ag0FADQv4VLPoJ7U/2dsb2JhbABRBgEDgww4Vqh1kn6BEBZ0giUBAQEETjcEAgEIEQMBAQELAhsHMQETAQkIAgQKCQgVBIdQAxENv30DYYR/F4xsCYEvCwEBEQ0GGxIFBguDE4EUBIkQjS2DGIYhhQqFO4MtgXE5 25, 30 -- X-IPAS-Result: Ag0FADQv4VLPoJ7U/2dsb2JhbABRBgEDgww4Vqh1kn6BEBZ0giUBAQEETjcEAgEIEQMBAQELAhsHMQETAQkIAgQKCQgVBIdQAxENv30DYYR/F4xsCYEvCwEBEQ0GGxIFBguDE4EUBIkQjS2DGIYhhQqFO4MtgXE5 25, 30 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 25, 30 -- by um-nip4-exch-relay.um.umsystem.edu with ESMTP; 23 Jan 2014 09:05:07 -0600 25, 30 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.195]) by 25, 30 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0169.001; 25, 30 -- Thu, 23 Jan 2014 09:05:06 -0600 25, 30 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 25, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 25, 30 -- Subject: RE: [Microscopy] Fw: Virus 25, 30 -- Thread-Topic: [Microscopy] Fw: Virus 25, 30 -- Thread-Index: AQHPGEaTIcrCG0fAIkeoPKD7Zb7QHZqSZ9IA 25, 30 -- Date: Thu, 23 Jan 2014 15:05:06 +0000 25, 30 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DBA1AD6-at-UM-MBX-T01.um.umsystem.edu} 25, 30 -- References: {201401231422.s0NEMeo7001595-at-ns.microscopy.com} 25, 30 -- In-Reply-To: {201401231422.s0NEMeo7001595-at-ns.microscopy.com} 25, 30 -- Accept-Language: en-US 25, 30 -- Content-Language: en-US 25, 30 -- X-MS-Has-Attach: 25, 30 -- X-MS-TNEF-Correlator: 25, 30 -- x-originating-ip: [134.193.156.65] 25, 30 -- Content-Type: text/plain; charset="iso-8859-1" 25, 30 -- MIME-Version: 1.0 25, 30 -- Content-Transfer-Encoding: 8bit 25, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NF58WE020492 ==============================End of - Headers==============================
A day without fusion is like a day without sunshine.
On 23/01/2014 11:05 AM, DusevichV-at-umkc.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I agree, it does not looks like belonging to section. Some contamination after sectioning/staining. I have stained a lot with PTA, have never seen something like this. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Director } (816) 235-2072 } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } -----Original Message----- } X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: Thursday, January 23, 2014 8:23 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Fw: Virus } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi Panos! } } Giving a picture without scale to a miscroscopist is like talking without words. We cannot make anything out if it! } I doubt these objects are embedded in the resin. One can see a hole in the section and one object seems to partly cover it. Also they don't look like they were sectioned. } Looks like artifacts for me. } } Regards, } Stephane } } } ________________________________ } X-from: "pveril-at-apae.uth.gr" {pveril-at-apae.uth.gr} } To: nizets2-at-yahoo.com } Sent: Thursday, January 23, 2014 11:29 AM } Subject: [Microscopy] Virus } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear all } } During an electron microscope examination of a Sparus aurata liver } collagen (PTA and uranyl acetate were used as stains) I came across to } these objects. They are probably virus particles. Can any one identify } them? } } http://upload.users.uth.gr/files/virus.jpg } } Many thanks } Panos
==============================Original Headers============================== 14, 21 -- From jehrman-at-mta.ca Thu Jan 23 09:21:14 2014 14, 21 -- Received: from smtpy.mta.ca (smtpy.mta.ca [138.73.1.143]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NFLEHO007014 14, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 09:21:14 -0600 14, 21 -- Received: from host-22-169.mta.ca ([138.73.22.169]:52005) 14, 21 -- by smtpy.mta.ca with esmtpsa (TLSv1:DHE-RSA-CAMELLIA256-SHA:256) 14, 21 -- (Exim 4.82) 14, 21 -- (envelope-from {jehrman-at-mta.ca} ) 14, 21 -- id 1W6M5g-0004Wn-CR 14, 21 -- for Microscopy-at-microscopy.com; Thu, 23 Jan 2014 11:21:12 -0400 14, 21 -- Message-ID: {52E13368.4060708-at-mta.ca} 14, 21 -- Date: Thu, 23 Jan 2014 11:21:12 -0400 14, 21 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 14, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 14, 21 -- MIME-Version: 1.0 14, 21 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 14, 21 -- Subject: Re: [Microscopy] RE: Fw: Virus 14, 21 -- References: {201401231505.s0NF5T6w021030-at-ns.microscopy.com} 14, 21 -- In-Reply-To: {201401231505.s0NF5T6w021030-at-ns.microscopy.com} 14, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Ya sou Panos Yes, scales are different, yet it is remarkable how much your particles resemble to pollen I have a picture from a wild flower pollen that looks very close to these particles http://www.eikonika.net/v2/downloads/KITSTI03_resize.jpg. Unlike in the North America, spring came earlier in Greece this year... Best wishes yorgos
A day without fusion is like a day without sunshine.
I agree, it does not looks like belonging to section. Some contamination after sectioning/staining. I have stained a lot with PTA, have never seen something like this.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, January 23, 2014 8:23 AM To: Dusevich, Vladimir
Hello Everyone, Years ago, maybe 20, I saw SEM images of material observed in diesel exhaust. It's not carbon black, but we never did figure out what it was. It look very much like these images. Any chance it could be environmental contamination?
I seriously doubt its pollen. Most pollen to too, much too big to see in it's entirety in a TEM.
I look forward to finding out what it is!
Frank
-----Original Message----- X-from: maryard-at-uga.edu [mailto:maryard-at-uga.edu] Sent: Thursday, January 23, 2014 9:18 AM To: Frank Karl
Panos,
What is the average size of the "particles"? You do not have a size bar in your image to get a feel for their size, which is important in any viral identification. The "particles" do not appear to be associated with the sample on the grid but laying on top of the sample. In my opinion, they look more like crystalline structures rather than viral particles. Depending on their actual size, could they possibly be phosphotungstic acid crystals?
Kind regards, Mary
Mary Ard Electron Microscopy Lab Coordinator Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, GA 30602 706-542-5537
==============================Original Headers============================== 6, 29 -- From maryard-at-uga.edu Thu Jan 23 08:06:42 2014 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NE6gO0014516 6, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 08:06:42 -0600 6, 29 -- Received: from DM2PR02MB480.namprd02.prod.outlook.com (10.141.84.156) by 6, 29 -- DM2PR02MB477.namprd02.prod.outlook.com (10.141.84.143) with Microsoft SMTP 6, 29 -- Server (TLS) id 15.0.859.15; Thu, 23 Jan 2014 14:06:37 +0000 6, 29 -- Received: from DM2PR02MB480.namprd02.prod.outlook.com ([10.141.84.156]) by 6, 29 -- DM2PR02MB480.namprd02.prod.outlook.com ([10.141.84.156]) with mapi id 6, 29 -- 15.00.0859.013; Thu, 23 Jan 2014 14:06:37 +0000 6, 29 -- From: Mary Brown Ard {maryard-at-uga.edu} 6, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 29 -- Subject: [Microscopy] Virus 6, 29 -- Thread-Topic: [Microscopy] Virus 6, 29 -- Thread-Index: Ac8YQxrT3GMwtR30RFK9YaSHozxtFQ== 6, 29 -- Date: Thu, 23 Jan 2014 14:06:36 +0000 6, 29 -- Message-ID: {473dbc6ac41041b38ff74dd69ab35549-at-DM2PR02MB480.namprd02.prod.outlook.com} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- x-originating-ip: [128.192.89.8] 6, 29 -- x-forefront-prvs: 0100732B76 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(71364002)(189002)(199002)(4396001)(74502001)(47446002)(79102001)(83322001)(2656002)(81686001)(81816001)(65816001)(80022001)(75432001)(59766001)(50986001)(85306002)(47976001)(66066001)(81542001)(87266001)(77982001)(33646001)(47736001)(49866001)(87936001)(63696002)(31966008)(92566001)(74662001)(81342001)(80976001)(74876001)(76576001)(76176001)(76786001)(76796001)(69226001)(85852003)(83072002)(74316001)(93516002)(86362001)(90146001)(93136001)(56816005)(74366001)(51856001)(46102001)(74706001)(54316002)(56776001)(53806001)(76482001)(54356001)(94316002)(24736002);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR02MB477;H:DM2PR02MB480.namprd02.prod.outlook.com;CLIP:128.192.89.8;FPR:;InfoNoRecordsMX:1;A:1;LANG:en; 6, 29 -- Content-Type: text/plain; charset="us-ascii" 6, 29 -- MIME-Version: 1.0 6, 29 -- X-OriginatorOrg: uga.edu 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NE6gO0014516 ==============================End of - Headers==============================
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==============================Original Headers============================== 19, 26 -- From frank_karl-at-ardl.com Thu Jan 23 11:34:30 2014 19, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 19, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NHYQtL014454 19, 26 -- for {microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 11:34:29 -0600 19, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 19, 26 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Thu, 23 19, 26 -- Jan 2014 12:34:25 -0500 19, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 19, 26 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 19, 26 -- {microscopy-at-microscopy.com} 19, 26 -- Date: Thu, 23 Jan 2014 12:34:25 -0500 19, 26 -- Subject: RE: [Microscopy] Virus 19, 26 -- Thread-Topic: [Microscopy] Virus 19, 26 -- Thread-Index: Ac8YRf7pmueARSLYRGOBttU8X/QlqwAGlBCw 19, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BC8C6CF65-at-exchange2k7.ad.ardl.com} 19, 26 -- References: {201401231417.s0NEHphn028174-at-ns.microscopy.com} 19, 26 -- In-Reply-To: {201401231417.s0NEHphn028174-at-ns.microscopy.com} 19, 26 -- Accept-Language: en-US 19, 26 -- Content-Language: en-US 19, 26 -- X-MS-Has-Attach: 19, 26 -- X-MS-TNEF-Correlator: 19, 26 -- acceptlanguage: en-US 19, 26 -- Content-Type: text/plain; charset="us-ascii" 19, 26 -- MIME-Version: 1.0 19, 26 -- Content-Transfer-Encoding: 8bit 19, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NHYQtL014454 ==============================End of - Headers==============================
A day without fusion is like a day without sunshine.
On 23/01/2014 11:05 AM, DusevichV-at-umkc.edu wrote: } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I agree, it does not looks like belonging to section. Some contamination after sectioning/staining. I have stained a lot with PTA, have never seen something like this. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Director } (816) 235-2072 } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } -----Original Message----- } X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: Thursday, January 23, 2014 8:23 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Fw: Virus } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } Hi Panos! } } Giving a picture without scale to a miscroscopist is like talking without words. We cannot make anything out if it! } I doubt these objects are embedded in the resin. One can see a hole in the section and one object seems to partly cover it. Also they don't look like they were sectioned. } Looks like artifacts for me. } } Regards, } Stephane } } } ________________________________ } X-from: "pveril-at-apae.uth.gr" {pveril-at-apae.uth.gr} } To: nizets2-at-yahoo.com } Sent: Thursday, January 23, 2014 11:29 AM } Subject: [Microscopy] Virus } } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } Dear all } } During an electron microscope examination of a Sparus aurata liver } collagen (PTA and uranyl acetate were used as stains) I came across to } these objects. They are probably virus particles. Can any one identify } them? } } http://upload.users.uth.gr/files/virus.jpg } } Many thanks } Panos
==============================Original Headers============================== 14, 21 -- From jehrman-at-mta.ca Thu Jan 23 09:21:14 2014 14, 21 -- Received: from smtpy.mta.ca (smtpy.mta.ca [138.73.1.143]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NFLEHO007014 14, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 09:21:14 -0600 14, 21 -- Received: from host-22-169.mta.ca ([138.73.22.169]:52005) 14, 21 -- by smtpy.mta.ca with esmtpsa (TLSv1:DHE-RSA-CAMELLIA256-SHA:256) 14, 21 -- (Exim 4.82) 14, 21 -- (envelope-from {jehrman-at-mta.ca} ) 14, 21 -- id 1W6M5g-0004Wn-CR 14, 21 -- for Microscopy-at-microscopy.com; Thu, 23 Jan 2014 11:21:12 -0400 14, 21 -- Message-ID: {52E13368.4060708-at-mta.ca} 14, 21 -- Date: Thu, 23 Jan 2014 11:21:12 -0400 14, 21 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 14, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 14, 21 -- MIME-Version: 1.0 14, 21 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 14, 21 -- Subject: Re: [Microscopy] RE: Fw: Virus 14, 21 -- References: {201401231505.s0NF5T6w021030-at-ns.microscopy.com} 14, 21 -- In-Reply-To: {201401231505.s0NF5T6w021030-at-ns.microscopy.com} 14, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 24, 30 -- From DusevichV-at-umkc.edu Thu Jan 23 11:58:05 2014 24, 30 -- Received: from um-nip3-umkc-out.um.umsystem.edu (um-nip3-umkc-out.um.umsystem.edu [198.209.49.166]) 24, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NHw4eZ001045 24, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 11:58:04 -0600 24, 30 -- X-IronPort-Anti-Spam-Filtered: true 24, 30 -- X-IronPort-Anti-Spam-Result: AhUFAIRX4VLPoJ7G/2dsb2JhbABRBwODDDhQBqh9kn6BExZ0giUBAQEEOhQYHwICAgEIEQMBAQEBCgISCQcbFgETAQMBBQgCBAoJCBUEh1ADEQgFwHWEfxcEjGgZgRkGCwEfBhsSBQYLgxOBFASWPYMYiysDhTiDLYFxOQ 24, 30 -- X-IPAS-Result: AhUFAIRX4VLPoJ7G/2dsb2JhbABRBwODDDhQBqh9kn6BExZ0giUBAQEEOhQYHwICAgEIEQMBAQEBCgISCQcbFgETAQMBBQgCBAoJCBUEh1ADEQgFwHWEfxcEjGgZgRkGCwEfBhsSBQYLgxOBFASWPYMYiysDhTiDLYFxOQ 24, 30 -- Received: from um-tcas2.um.umsystem.edu ([207.160.158.198]) 24, 30 -- by um-nip3-exch-relay.um.umsystem.edu with ESMTP; 23 Jan 2014 11:58:03 -0600 24, 30 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.195]) by 24, 30 -- UM-TCAS2.um.umsystem.edu ([207.160.158.198]) with mapi id 14.03.0169.001; 24, 30 -- Thu, 23 Jan 2014 11:58:03 -0600 24, 30 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 24, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 24, 30 -- Subject: RE: [Microscopy] Fw: Virus 24, 30 -- Thread-Topic: [Microscopy] Fw: Virus 24, 30 -- Thread-Index: AQHPGE7PtrREBGJTgUK+m2nXye/P95qSmFQA 24, 30 -- Date: Thu, 23 Jan 2014 17:58:03 +0000 24, 30 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DBA1B68-at-UM-MBX-T01.um.umsystem.edu} 24, 30 -- References: {201401231521.s0NFLa4x007680-at-ns.microscopy.com} 24, 30 -- In-Reply-To: {201401231521.s0NFLa4x007680-at-ns.microscopy.com} 24, 30 -- Accept-Language: en-US 24, 30 -- Content-Language: en-US 24, 30 -- X-MS-Has-Attach: 24, 30 -- X-MS-TNEF-Correlator: 24, 30 -- x-originating-ip: [134.193.156.65] 24, 30 -- Content-Type: text/plain; charset="us-ascii" 24, 30 -- MIME-Version: 1.0 24, 30 -- Content-Transfer-Encoding: 8bit 24, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NHw4eZ001045 ==============================End of - Headers==============================
I take it that these are sitting on a film and are negatively stained? With BOTH UrAc and PTA? They look vaguely like something tentatively identified as scales from some sort of phytoplankton that we often see in marine preps. They do not look like any of the many marine viruses we've seen here, but I will show them to someone who works on marine viruses and see what they say. However, right now I'm going with the idea that they are stain crystals!
Aloha, Tina
} During an electron microscope examination of a Sparus aurata liver } collagen (PTA and uranyl acetate were used as stains) I came across to } these objects. They are probably virus particles. Can any one identify } them? } } http://upload.users.uth.gr/files/virus.jpg } } Many thanks } Panos } -- } Dr. Berillis Panagiotis } Lecturer in Microscopy, image analysis and histology } Department of Ichthyology and Aquatic Environment } Agricultural school } University of Thessaly } } } ==============================Original Headers============================== } 6, 18 -- From pveril-at-apae.uth.gr Thu Jan 23 04:23:12 2014 } 6, 18 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NANBUa024847 } 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 04:23:12 -0600 } 6, 18 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) } 6, 18 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0NANwCR031164 } 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 12:23:58 +0200 } 6, 18 -- Received: from 10.64.60.150 ([10.64.60.150]) by webmail.uth.gr (Horde } 6, 18 -- Framework) with HTTP; Thu, 23 Jan 2014 12:23:00 +0200 } 6, 18 -- Date: Thu, 23 Jan 2014 12:23:00 +0200 } 6, 18 -- Message-ID: {20140123122300.Horde.--gLtdj-JhwWKO_juLlJ4w3-at-webmail.uth.gr} } 6, 18 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} } 6, 18 -- To: Microscopy-at-microscopy.com } 6, 18 -- Subject: Virus } 6, 18 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) } 6, 18 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes } 6, 18 -- MIME-Version: 1.0 } 6, 18 -- Content-Disposition: inline } ==============================End of - Headers============================== } }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 23 -- From tina-at-pbrc.hawaii.edu Thu Jan 23 12:46:43 2014 5, 23 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NIkh1E020397 5, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 12:46:43 -0600 5, 23 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 23 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id s0NIjs4K020773 5, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 23 -- Thu, 23 Jan 2014 08:45:54 -1000 (HST) 5, 23 -- Received: from localhost (tina-at-localhost) 5, 23 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id s0NIjo2Q020738; 5, 23 -- Thu, 23 Jan 2014 08:45:54 -1000 (HST) 5, 23 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 5, 23 -- Date: Thu, 23 Jan 2014 08:45:50 -1000 (HST) 5, 23 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 23 -- X-X-Sender: tina-at-sf-v240 5, 23 -- To: pveril-at-apae.uth.gr 5, 23 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 23 -- Subject: Re: [Microscopy] Virus 5, 23 -- In-Reply-To: {201401231025.s0NAPGdU025978-at-ns.microscopy.com} 5, 23 -- Message-ID: {Pine.GSO.4.64.1401230841250.19887-at-sf-v240} 5, 23 -- References: {201401231025.s0NAPGdU025978-at-ns.microscopy.com} 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Yes, pollen was my immediate thought, but pollen would be at least one, probably two, order/s of magnitude too big!
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 24/01/14 4:02 AM, "eikonika-at-otenet.gr" {eikonika-at-otenet.gr} wrote:
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We agree with those people who think that the "bubbles" have to do with something extracted from the bacteria that prevents the resin from polymerising.
We did not use osmium, we dehydrated in ethanol up to 80% ethanol and did not degas the resin. We used a premix resin that works OK with any other specimens except bacteria. But we do not add the extra accelerator.
Perhaps the next steps will be: (i) to dehydrate up to 90 % alcohol (ii) degas the resin (iii) add extra accelerator so that whatever chemical reaction we have might not occur? Any suggestions are welcome and thank you again!
Stefan
==============================Original Headers============================== 5, 30 -- From stefan.wernitznig-at-medunigraz.at Fri Jan 24 04:43:33 2014 5, 30 -- Received: from si068.medunigraz.at (si083.medunigraz.at [193.170.105.83]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0OAhW8L003061 5, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 04:43:33 -0600 5, 30 -- X-AuditID: 0ac80c44-f79eb6d000000e72-57-52e243d3360a 5, 30 -- Received: from si062.medunigraz.at (Unknown_Domain [10.200.18.79]) 5, 30 -- by si068.medunigraz.at (Symantec Mail Security) with SMTP id D2.BC.03698.3D342E25; Fri, 24 Jan 2014 11:43:31 +0100 (CET) 5, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at [10.200.112.128]) 5, 30 -- by si062.medunigraz.at with ESMTP (TLS encrypted); Fri, 24 Jan 2014 11:43:05 +0100 5, 30 -- Message-ID: {52E243B9.3030209-at-medunigraz.at} 5, 30 -- Date: Fri, 24 Jan 2014 11:43:05 +0100 5, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} 5, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 5, 30 -- MIME-Version: 1.0 5, 30 -- To: Microscopy-at-microscopy.com 5, 30 -- Subject: Re: Strange bubbles in LR white resin 5, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 5, 30 -- Content-Transfer-Encoding: 7bit 5, 30 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFlrDIsWRmVeSWpSXmKPExsXCdULIX/ey86Mgg6N9bBZ/DjewOzB6fOx+ 5, 30 -- zRTAGMVlk5Kak1mWWqRvl8CVse7KcfaCJawVDU9usjYwTmPpYuTkkBAwkZiw+SQbhC0mceHe 5, 30 -- eiCbi0NIYC+jxKOmd4wQzjxGiU9rLjGBVPEK6Eg0vOgAs1kEVCU2N1xlBLHZBCwljnf/AJsq 5, 30 -- KhAssfvxM1aIekGJkzOfgMVFBGQlFrw/wg5iCwvoSdxqugI2h1nARuLviUUsELa8xPa3c5gn 5, 30 -- MPLOQtI+C0nZLCRlCxiZVzEKF2camFno5aamlOZlphclVukllmxiBAcOj8sOxoYthocYBTgY 5, 30 -- lXh46y0fBQmxJpYVV+YeYpTkYFIS5X3qABTiS8pPqcxILM6ILyrNSS0+xCjBwawkwlt/7WGQ 5, 30 -- EG9KYmVValE+TEqag0VJnHeLxN0gIYH0xJLU7NTUgtQimKwMB4eSBG+/E9BQwaLU9NSKtMyc 5, 30 -- EoQ0EwcnyHAeoOGXQGp4iwsSc4sz0yHypxglpcR5w0ASAiCJjNI8uN5XjOJALwjzvgDJ8gCT 5, 30 -- AFzXK6CBTEADV5x9ADKwJBEhJdXA6GD0s//epWpOoYWpBd0HKoK/vJb6e3K6zp+pYlX7+GwK 5, 30 -- hQ7EX6j3fsc0I9DggzhbsepUI601LNlveU4++937Yc/VaYnK7FE3Wdv/HPM9fyKIUTbNesIG 5, 30 -- Ec3zR+TEvH3Wnyytnv4mRsQt1+SVnJ3YDcVDxj8LJriYMeT/mz75u6/4Oc18YR95JZbijERD 5, 30 -- Leai4kQAR3Z+l78CAAA= ==============================End of - Headers==============================
Since bacteria are usually plentiful and inexpensive, I would also do one sample after 100% ethanol to see if it is do to residual water. Degassing the resin can change composition since you can differentially extract the more volatile components. Tom Phillips
Sent from my iPad
} On Jan 24, 2014, at 4:44 AM, "stefan.wernitznig-at-medunigraz.at" {stefan.wernitznig-at-medunigraz.at} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thank you for the intense & helpful discussion! } } We agree with those people who think that the "bubbles" have to do with } something extracted from the bacteria that prevents the resin from } polymerising. } } We did not use osmium, we dehydrated in ethanol up to 80% ethanol and } did not degas the resin. We used a premix resin that works OK with any } other specimens except bacteria. But we do not add the extra accelerator. } } Perhaps the next steps will be: } (i) to dehydrate up to 90 % alcohol } (ii) degas the resin } (iii) add extra accelerator so that whatever chemical reaction we have } might not occur? } Any suggestions are welcome and thank you again! } } Stefan } } ==============================Original Headers============================== } 5, 30 -- From stefan.wernitznig-at-medunigraz.at Fri Jan 24 04:43:33 2014 } 5, 30 -- Received: from si068.medunigraz.at (si083.medunigraz.at [193.170.105.83]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0OAhW8L003061 } 5, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 04:43:33 -0600 } 5, 30 -- X-AuditID: 0ac80c44-f79eb6d000000e72-57-52e243d3360a } 5, 30 -- Received: from si062.medunigraz.at (Unknown_Domain [10.200.18.79]) } 5, 30 -- by si068.medunigraz.at (Symantec Mail Security) with SMTP id D2.BC.03698.3D342E25; Fri, 24 Jan 2014 11:43:31 +0100 (CET) } 5, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at [10.200.112.128]) } 5, 30 -- by si062.medunigraz.at with ESMTP (TLS encrypted); Fri, 24 Jan 2014 11:43:05 +0100 } 5, 30 -- Message-ID: {52E243B9.3030209-at-medunigraz.at} } 5, 30 -- Date: Fri, 24 Jan 2014 11:43:05 +0100 } 5, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} } 5, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 } 5, 30 -- MIME-Version: 1.0 } 5, 30 -- To: Microscopy-at-microscopy.com } 5, 30 -- Subject: Re: Strange bubbles in LR white resin } 5, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 5, 30 -- Content-Transfer-Encoding: 7bit } 5, 30 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFlrDIsWRmVeSWpSXmKPExsXCdULIX/ey86Mgg6N9bBZ/DjewOzB6fOx+ } 5, 30 -- zRTAGMVlk5Kak1mWWqRvl8CVse7KcfaCJawVDU9usjYwTmPpYuTkkBAwkZiw+SQbhC0mceHe } 5, 30 -- eiCbi0NIYC+jxKOmd4wQzjxGiU9rLjGBVPEK6Eg0vOgAs1kEVCU2N1xlBLHZBCwljnf/AJsq } 5, 30 -- KhAssfvxM1aIekGJkzOfgMVFBGQlFrw/wg5iCwvoSdxqugI2h1nARuLviUUsELa8xPa3c5gn } 5, 30 -- MPLOQtI+C0nZLCRlCxiZVzEKF2camFno5aamlOZlphclVukllmxiBAcOj8sOxoYthocYBTgY } 5, 30 -- lXh46y0fBQmxJpYVV+YeYpTkYFIS5X3qABTiS8pPqcxILM6ILyrNSS0+xCjBwawkwlt/7WGQ } 5, 30 -- EG9KYmVValE+TEqag0VJnHeLxN0gIYH0xJLU7NTUgtQimKwMB4eSBG+/E9BQwaLU9NSKtMyc } 5, 30 -- EoQ0EwcnyHAeoOGXQGp4iwsSc4sz0yHypxglpcR5w0ASAiCJjNI8uN5XjOJALwjzvgDJ8gCT } 5, 30 -- AFzXK6CBTEADV5x9ADKwJBEhJdXA6GD0s//epWpOoYWpBd0HKoK/vJb6e3K6zp+pYlX7+GwK } 5, 30 -- hQ7EX6j3fsc0I9DggzhbsepUI601LNlveU4++937Yc/VaYnK7FE3Wdv/HPM9fyKIUTbNesIG } 5, 30 -- Ec3zR+TEvH3Wnyytnv4mRsQt1+SVnJ3YDcVDxj8LJriYMeT/mz75u6/4Oc18YR95JZbijERD } 5, 30 -- Leai4kQAR3Z+l78CAAA= } ==============================End of - Headers==============================
==============================Original Headers============================== 4, 30 -- From PhillipsT-at-missouri.edu Fri Jan 24 07:35:00 2014 4, 30 -- Received: from mst-rip5-missouri-out.um.umsystem.edu (mst-rip5-missouri-out.um.umsystem.edu [198.209.50.135]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0ODYxOo023666 4, 30 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:34:59 -0600 4, 30 -- X-IronPort-Anti-Spam-Filtered: true 4, 30 -- X-IronPort-Anti-Spam-Result: Ag4FAF9r4lLPoJ7U/2dsb2JhbABagww4qkiSRIELFnSCJQEBAQMBOhQwCwIBCBEDAQIBDBIQMQEXAQUIAgQBCQkdBIdcCA3DFYR/F447AQEcCC0FBoMegRQEmVmQbIMtPIE1 4, 30 -- X-IPAS-Result: Ag4FAF9r4lLPoJ7U/2dsb2JhbABagww4qkiSRIELFnSCJQEBAQMBOhQwCwIBCBEDAQIBDBIQMQEXAQUIAgQBCQkdBIdcCA3DFYR/F447AQEcCC0FBoMegRQEmVmQbIMtPIE1 4, 30 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 4, 30 -- by mst-rip5-exch-relay.um.umsystem.edu with ESMTP; 24 Jan 2014 07:34:58 -0600 4, 30 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.204]) by 4, 30 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0169.001; 4, 30 -- Fri, 24 Jan 2014 07:34:58 -0600 4, 30 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 4, 30 -- To: " {stefan.wernitznig-at-medunigraz.at} " {stefan.wernitznig-at-medunigraz.at} , 4, 30 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 30 -- Subject: Re: [Microscopy] Re: Strange bubbles in LR white resin 4, 30 -- Thread-Topic: [Microscopy] Re: Strange bubbles in LR white resin 4, 30 -- Thread-Index: AQHPGPFAH2FL5R+ITUCOPheQKH+i8pqT4ENp 4, 30 -- Date: Fri, 24 Jan 2014 13:34:58 +0000 4, 30 -- Message-ID: {39C2E286-2C85-4550-999C-DF01A748F01A-at-missouri.edu} 4, 30 -- References: {201401241044.s0OAiOuv003904-at-ns.microscopy.com} 4, 30 -- In-Reply-To: {201401241044.s0OAiOuv003904-at-ns.microscopy.com} 4, 30 -- Accept-Language: en-US 4, 30 -- Content-Language: en-US 4, 30 -- X-MS-Has-Attach: 4, 30 -- X-MS-TNEF-Correlator: 4, 30 -- Content-Type: text/plain; charset="us-ascii" 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Transfer-Encoding: 8bit 4, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0ODYxOo023666 ==============================End of - Headers==============================
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] TEM Diffraction of amphibole fibers and fiber bundles
Message: Question for any Mineralogist-Microscopist (or the other way 'round :) ) in the group.
I have a Mineralogy student working in my lab on the TEM trying to obtain diffraction patterns (SAED) on amphibole fibers and fiber bundles (note: some of the specimens may be cleavage fragments). Particle size: 50-100 microns short dimension & 100s if microns in the long D. Scope JEOL 2010 at 200kV.
We are having a surprising difficult time getting any reasonable SAED patterns on these. We can get reasonable and nice patterns on adjacent micas in the sample but nothing of use on the amphiboles. Different spot sizes, SAED apertures, camera lengths, etc. No joy.
We should be able to do this-even though I am not the worlds best at electron diffraction?? I'm at a loss and throwing myself Forrest Gump like asking for mercy!
Suggestions would be welcome.
Thanks-Tom
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 24 07:41:08 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0ODf87s031166 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:41:08 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id E737C6AED43 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:41:08 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id 9AmVeM06qm3E for {microscopy-at-microscopy.com} ; 19, 28 -- Fri, 24 Jan 2014 07:41:07 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id B5E306AED31 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:41:07 -0600 (CST) 19, 28 -- Message-ID: {52E26D73.4030707-at-microscopy.com} 19, 28 -- Date: Fri, 24 Jan 2014 07:41:07 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:TEM Diffraction of amphibole fibers and fiber bundles 19, 28 -- References: {201401231858.s0NIwMiT006807-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201401231858.s0NIwMiT006807-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201401231858.s0NIwMiT006807-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Tom, 50-100 µm is very thick for SAED. You are probably not getting the electrons through the sample, whereas, the micas are probably thinner. The thing to try is getting diffraction patterns from the very thinnest edges of the fibers. You could crush the sample further, but that would destroy the measurement of length-width aspect. If you have EDX, you can correlate composition of the crushed sample patterns to as-received samples. Hope this helps. Ken
|_|"|_|"|_|_|"|_|"|_|"|_|_|"|_|"|_|_|"|_|"|_|"|_|_| Kenneth JT Livi, PhD Director, The High-Resolution Analytical Electron Microbeam Facility of the Integrated Imaging Center Departments of Earth and Planetary Sciences and Biology Olin Hall 3400 N Charles Street Johns Hopkins University Baltimore, Maryland 21218 USA | | | .| | .| | .| | .| | | :| | | .| | .| | .| | .| | | :|
==============================Original Headers============================== 11, 19 -- From prvs=0942b11ae=klivi-at-jhu.edu Fri Jan 24 07:53:57 2014 11, 19 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.150]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0ODrv7U023753 11, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:53:57 -0600 11, 19 -- X-IronPort-AV: E=Sophos;i="4.95,712,1384318800"; 11, 19 -- d="scan'208";a="21952747" 11, 19 -- Received: from unknown (HELO [128.220.60.54]) ([128.220.60.54]) 11, 19 -- by ipex0.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 24 Jan 2014 08:53:53 -0500 11, 19 -- From: Ken Livi {klivi-at-jhu.edu} 11, 19 -- Content-Type: text/plain; charset=iso-8859-1 11, 19 -- Subject: [Microscopy] viaWWW:TEM Diffraction of amphibole fibers and fiber bundles 11, 19 -- Date: Fri, 24 Jan 2014 08:53:52 -0500 11, 19 -- Message-Id: {3BB2F6B4-3AC5-443D-A34E-B9E684018256-at-jhu.edu} 11, 19 -- Cc: Microscopy-at-microscopy.com 11, 19 -- To: tomw-at-uidaho.edu 11, 19 -- Mime-Version: 1.0 (Apple Message framework v1283) 11, 19 -- X-Mailer: Apple Mail (2.1283) 11, 19 -- Content-Transfer-Encoding: 8bit 11, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0ODrv7U023753 ==============================End of - Headers==============================
the samples are thin enough for TEM? Your description sounds a lot like the fibers are too thick, they need to be less than half a micron thick to allow you a chance to get results. Cleavage is a great technique but you might need to do it twenty times to get something decent.
Good luck Rob
-- Robert Keyse, DPhil Research Scientist Lehigh University
5, East Packer Avenue, Whitaker Laboratory Bethlehem, PA 18015-3194
Office (610)758-3465 Fax (610)758-4244
==============================Original Headers============================== 7, 21 -- From rok210-at-lehigh.edu Fri Jan 24 08:03:18 2014 7, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0OE3IZT005256 7, 21 -- for {Microscopy-at-Microscopy.com} ; Fri, 24 Jan 2014 08:03:18 -0600 7, 21 -- Received: from [127.0.0.1] (Dyn055015.mat.Lehigh.EDU [128.180.55.15]) 7, 21 -- (authenticated bits=0) 7, 21 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id s0OE3HCQ009183 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 7, 21 -- Fri, 24 Jan 2014 09:03:18 -0500 7, 21 -- Message-ID: {52E272A5.5030207-at-lehigh.edu} 7, 21 -- Date: Fri, 24 Jan 2014 09:03:17 -0500 7, 21 -- From: Robert Keyse {rok210-at-lehigh.edu} 7, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 7, 21 -- MIME-Version: 1.0 7, 21 -- To: Microscopy-at-Microscopy.com 7, 21 -- CC: tomw-at-uidaho.edu 7, 21 -- Subject: TEM Diffraction of amphibole fibers and fiber bundles 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Virus-Scanned: clamav-milter 0.98 at rain.cc.lehigh.edu 7, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I do not remember seeing how long your steps were during dehydration in ethanol but do know that these little bacteria take longer than anticipated to dehydrate. I have also found it advantageous to have the ethanol moving by putting the tubes on a tilting table or to rotate them during dehydration and also for the infiltration steps.
I have had similar looking bacteria in Macrophages embedded in EPON in the past. After the first paper was published I learned that my problem was their dehydration. The macrophages themselves looked fine when processed by my standard tissue culture protocol but the Listeria within them turned very dark and peppered like yours when the beam hit them. After I started to use our longer protocol for tissue samples, the bacteria looked great.
Many years ago I was advised to go through 90% ethanol when using LR White so your suggestion to do that is a good one.
One can introduce air/oxygen into the embedding media when stirring in the accelerator so degassing could help in the elimination of that possibility.
I usually harden the LR White in a 45 degree C. oven for a few days without accelerator so I can not address the possibility of adding an additional amount of accelerator.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Pat
Patricia Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-nhlbi.nih.gov
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In bright field look for areas that exhibit bend contours. Those are the areas that are thin enough where you can find a reasonable pattern usually at the terminations.
Find fibers that are elongated in the direction of the tilt axis, adjust the height of the sample so you can tilt without the fiber moving.
Tilting about the long axis which is [001] you can see the closely spaced spots of 010.
Tilting about 010, ideally you should find [100] and [101] about 30° apart.
Easy to find in the orthorhombic amphiboles and less easy in the monoclinic amphiboles.
I suggest practicing with iucc standards. Tremolite and anthophyllite.
Also with Dave Palmer's Single Crystal program you don't need a TEM.
Getting indexable diffraction patterns off fibrous amphiboles requires patience which is why asbestos labs don't do it.
Gordon --------------------------------------------------- Gordon L. Nord, Ph. D Senior Scientist International Environmental Research Foundation P.O. Box 3459, Grand Central Station New York, NY 10163-3459
{http://www.ierfinc.org/}
==============================Original Headers============================== 17, 25 -- From gnord-at-mindspring.com Fri Jan 24 13:45:11 2014 17, 25 -- Received: from elasmtp-dupuy.atl.sa.earthlink.net (elasmtp-dupuy.atl.sa.earthlink.net [209.86.89.62]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0OJjBTo024549 17, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Jan 2014 13:45:11 -0600 17, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 17, 25 -- s=dk20050327; d=mindspring.com; 17, 25 -- b=OSWu1aeZGRUOdW3AAquhk8Vm8my/3TybTvBaKzLA6+ILxVddTOmwEYjeoL2n3Di8; 17, 25 -- h=Received:From:Content-Type:Content-Transfer-Encoding:Subject:Date:Message-Id:To:Mime-Version:X-Mailer:X-ELNK-Trace:X-Originating-IP; 17, 25 -- Received: from [96.247.192.239] (helo=earth.home) 17, 25 -- by elasmtp-dupuy.atl.sa.earthlink.net with esmtpa (Exim 4.67) 17, 25 -- (envelope-from {gnord-at-mindspring.com} ) 17, 25 -- id 1W6mgW-0003le-Os 17, 25 -- for Microscopy-at-Microscopy.Com; Fri, 24 Jan 2014 14:45:00 -0500 17, 25 -- From: Gordon Nord {gnord-at-mindspring.com} 17, 25 -- Content-Type: text/plain; charset=iso-8859-1 17, 25 -- Subject: Re: [Microscopy] viaWWW:TEM Diffraction of amphibole fibers and fiber bundles 17, 25 -- Date: Fri, 24 Jan 2014 14:44:59 -0500 17, 25 -- Message-Id: {0A8C9829-63C3-4296-BDE3-DE92A1466AF8-at-mindspring.com} 17, 25 -- To: Microscopy-at-Microscopy.Com 17, 25 -- Mime-Version: 1.0 (Apple Message framework v1085) 17, 25 -- X-Mailer: Apple Mail (2.1085) 17, 25 -- X-ELNK-Trace: 3df33169c923931ad4c20f6b8d69d88891b749bef7332bbc58b6c049e7d0e0da6c68a24449faf2cf350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 17, 25 -- X-Originating-IP: 96.247.192.239 17, 25 -- Content-Transfer-Encoding: 8bit 17, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0OJjBTo024549 ==============================End of - Headers==============================
The objects you have seen under your EM are not virus or pollen, They are Brochosomes. Brochosomes are secretory granules produced by the Malphigian tubules of leafhoppers. You may have a look at the Wikipedia article concerning this unfamiliar but beautiful structure (wikipedia.org/wiki/Brochosome) . I have seen EM pictures of this structure many years ago , since it has been once described in the sixties by my former colleagues in the lab in Rennes (France, Brittany). (Gouranton J. & Maillet P.L. (1967) Origine et structure des brochosomes. Journal de Microscopie 6: 53-64.). Brochosomes are source of contaminations and can sometimes be found in aerosol (Wiffen R. D. & Heard M. J. (1969) Unidentified airborn species. Nature 224: 715). I recommend the readings associated with the Wiki article. However, if you are interested, I can look over the archives of the lab and try to find original negatives.
Daniel
Daniel THOMAS UMR CNRS 6290 Universite de Rennes 1 35042 RENNES, FRANCE
==============================Original Headers============================== 4, 17 -- From daniel.thomas-at-univ-rennes1.fr Sat Jan 25 05:46:16 2014 4, 17 -- Received: from smtp.smtpout.orange.fr (smtp05.smtpout.orange.fr [80.12.242.127]) 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0PBkFNJ002358 4, 17 -- for {microscopy-at-microscopy.com} ; Sat, 25 Jan 2014 05:46:15 -0600 4, 17 -- Received: from air-de-daniel.home ([2.10.186.254]) 4, 17 -- by mwinf5d10 with ME 4, 17 -- id JBmE1n0025VlCY203BmExC; Sat, 25 Jan 2014 12:46:14 +0100 4, 17 -- From: Daniel Thomas {daniel.thomas-at-univ-rennes1.fr} 4, 17 -- Content-Type: text/plain; charset=us-ascii 4, 17 -- Subject: EM Virus 4, 17 -- Message-Id: {4A7927F1-9876-462E-BFCE-E4042BF2F08B-at-univ-rennes1.fr} 4, 17 -- Date: Sat, 25 Jan 2014 12:46:13 +0100 4, 17 -- To: microscopy-at-microscopy.com 4, 17 -- Mime-Version: 1.0 (Mac OS X Mail 7.1 \(1827\)) 4, 17 -- X-Mailer: Apple Mail (2.1827) 4, 17 -- Content-Transfer-Encoding: 8bit 4, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0PBkFNJ002358 ==============================End of - Headers==============================
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Merci Daniel for showing these brochosomes, they have a very smart shape. A pattern repeated in many structures seen at different scales in this wonderful world. And I am sure you gave the right answer.
----- Original Message ----- X-from: {daniel.thomas-at-univ-rennes1.fr} To: {eikonika-at-otenet.gr} Sent: Saturday, January 25, 2014 1:55 PM
March 24-28, 2014 a course in scanning electron microscopy will be offered at Hooke College of Applied Sciences. Further information about this hands-on course can be found at the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax) www.hookecollege.com
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From medications_canadian7-at-cyfrowypolsat.pl Tue Jan 28 11:58:16 2014 Return-Path: {medications_canadian7-at-cyfrowypolsat.pl} Received: from ip-37-109-125-139.multi.internet.cyfrowypolsat.pl (ip-37-109-125-139.multi.internet.cyfrowypolsat.pl [37.109.125.139]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0SHwAJp018396 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 28 Jan 2014 11:58:13 -0600 Message-Id: {201401281758.s0SHwAJp018396-at-ns.microscopy.com}
We have an old Zygo optical profilometer that we are looking to get rid of. The microscope works fine but the computer to which it is attached is not operational as no one remembers the root password and we don't have the operating system tapes to re-install from, it runs HP Unix. I have taken some photos and they can be viewed at;
Again the item is in Minneapolis, MN and it is free but the buyer must assume all shipping costs.
Nick Seaton University of Minnesota Characterization Facility
--
Dr Nicholas Seaton
SEM Specialist Characterization Facility
College of Science and Engineering
University of Minnesota
Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
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From pharmacy_reasonable7-at-kidsforsavingearth.org Wed Jan 29 01:07:16 2014 Return-Path: {pharmacy_reasonable7-at-kidsforsavingearth.org} Received: from mail3.kidsforsavingearth.org (46.191.166.37.dynamic.ufanet.ru [46.191.166.37] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0T779rp019831 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 29 Jan 2014 01:07:11 -0600 Message-Id: {201401290707.s0T779rp019831-at-ns.microscopy.com}
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Role of CaCl2 in biological sample fixation for TEM
Message: What is the role of CaCl2 in biological sample fixation for TEM.? What is the recommended concentration required in phosphate buffer.?
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Ravi, Calcium and magnesium chloride are used in TEM fixatives to protect membranes from enzymes that are still active during fixation. This is refered to as maintaining membrane tonicity (small breaks in membranes may occur). If phosphate buffers are used, you must use only MgCl2 (3 mM), as calcium will precipitate to calcium phosphate.
sincerely, Michael Delannoy Johns Hopkins SOM Microscope Facility
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X-from: joseph.n.madary.civ-at-mail.mil ()
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Email: joseph.n.madary.civ-at-mail.mil Name: Nick Madary
Message: Has anyone out there actually seen how the diamond knives are resharpened? I wonder if there is a tool and a training to attempt it. We have so many and it is difficult to buy them for us. Anyone also know of a compnmay that takes several knives in exchange for a knife? Like giving up 3-5 knives for a new one?
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==============================Original Headers============================== 10, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 29 13:47:57 2014 10, 28 -- Received: from znl.com ([206.69.208.20]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0TJlv0d007832 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jan 2014 13:47:57 -0600 10, 28 -- Received: from localhost (localhost [127.0.0.1]) 10, 28 -- by znl.com (Postfix) with ESMTP id B8ECA6B24ED 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jan 2014 13:47:57 -0600 (CST) 10, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 28 -- Received: from znl.com ([127.0.0.1]) 10, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id DqKf3XFmhRUn for {microscopy-at-microscopy.com} ; 10, 28 -- Wed, 29 Jan 2014 13:47:56 -0600 (CST) 10, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 10, 28 -- by znl.com (Postfix) with ESMTPA id 58C246B24E0 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jan 2014 13:47:56 -0600 (CST) 10, 28 -- Message-ID: {52E95AEC.9050101-at-microscopy.com} 10, 28 -- Date: Wed, 29 Jan 2014 13:47:56 -0600 10, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 10, 28 -- MIME-Version: 1.0 10, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 28 -- Subject: viaWWW:Diamond knife sharpening 10, 28 -- References: {201401291801.s0TI1eCA006638-at-ns.microscopy.com} 10, 28 -- In-Reply-To: {201401291801.s0TI1eCA006638-at-ns.microscopy.com} 10, 28 -- X-Forwarded-Message-Id: {201401291801.s0TI1eCA006638-at-ns.microscopy.com} 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Working on an issue that has come up in my Leo 1450VPSE SEM since I changed a gun filament. Seeing light and dark scan lines in captured images. First thought it's some kind of charging behavior but not sure. Problem occurs with both SE and BS detectors as I try to capture an image using the LINE AVG mode where a line of the image is scanned a number of times (N) before moving to the next line. This builds my image one scan line at a time till I have a full frame. Using scan speed six (rate it scans frame) I get different widths of shadow line as I change N.
Since I cannot attach images, I can Email them direct to anyone that may want to offer a suggestion as to what is causing the problem.
Thanks
Roy Beavers Southern Methodist University rbeavers-at-smu.edu
==============================Original Headers============================== 6, 28 -- From rbeavers-at-mail.smu.edu Wed Jan 29 14:00:04 2014 6, 28 -- Received: from smtap2.systems.smu.edu (smtap2.systems.smu.edu [129.119.65.151]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0TK04ig023009 6, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Jan 2014 14:00:04 -0600 6, 28 -- X-IronPort-Anti-Spam-Filtered: true 6, 28 -- X-IronPort-Anti-Spam-Result: AgsFAGRc6VKBd0GG/2dsb2JhbABZgmshgQ69DIEHFnSCJwU6UQEqFEImAQQTCId9AZtPpxCHMxeOHREBH4NcgRQEqkeDLYFxOQ 6, 28 -- X-IPAS-Result: AgsFAGRc6VKBd0GG/2dsb2JhbABZgmshgQ69DIEHFnSCJwU6UQEqFEImAQQTCId9AZtPpxCHMxeOHREBH4NcgRQEqkeDLYFxOQ 6, 28 -- Received: from sxht1p3.systems.smu.edu ([129.119.65.134]) 6, 28 -- by smtah2.systems.smu.edu with ESMTP/TLS/AES128-SHA; 29 Jan 2014 14:00:04 -0600 6, 28 -- Received: from SXMB1PG.SYSTEMS.SMU.EDU ([fe80::1449:c186:5adb:ddbb]) by 6, 28 -- SXHT1P3.SYSTEMS.SMU.EDU ([fe80::4479:3bff:ca05:ebce%17]) with mapi id 6, 28 -- 14.02.0387.000; Wed, 29 Jan 2014 14:00:03 -0600 6, 28 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 6, 28 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 28 -- Subject: Help with scan line problem in Leo 1450VPSE 6, 28 -- Thread-Topic: Help with scan line problem in Leo 1450VPSE 6, 28 -- Thread-Index: Ac8dLIfYOqs5okgrTjuz906249AkOQ== 6, 28 -- Date: Wed, 29 Jan 2014 20:00:03 +0000 6, 28 -- Message-ID: {BC0C36E74D341741A25CE2ABB122E7A330AF551D-at-SXMB1PG.SYSTEMS.SMU.EDU} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- x-originating-ip: [129.119.65.139] 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0TK04ig023009 ==============================End of - Headers==============================
Nick, Diatome diamond knives will replace a used knife (any size) for a brand new knife (any size) at half the cost of a new knife. Believe me, this is the best deal I know of. If you find a better one, let us all know.
sincerely, Michael Delannoy Johns Hopkins SOM Microscope Facility
----- Original Message ----- X-from: microscopylistserver-noreply-at-microscopy.com
Nick,
Years ago, a knife vendor gave a talk on how diamond knives are sharpened. It is not hard, but takes 1-2 weeks of grinding on a diamond grinding wheel. This in not something that you can do in your leisure. I once asked another vendor this same question about giving them 3 or four knives and get a new sharpened knife in return. After picking themselves off the ground from laughing so hard, they explained to me that the diamond is about 40-50 dollars, it is the time it takes to sharpen the knife that cost so much, so they will not just give a sharpened knife with exchange of 3 or 4 knives. One of the vendors does give a discount for a 2 for 1 exchange. Call them up and ask about this.
Best of luck convincing the powers that be to purchase a knife for you.
Ed
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, January 29, 2014 2:52 PM To: Calomeni, Edward
X-from: duraine-at-bcm.edu ()
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Message: Diatome has a deal where if you send in three knives for re-sharpening, they will charge for two of them at the re-sharpen price and the third one is free.
Since you have many knives it might be advantageous to keep a few sets of three so when you send one set in you will have another to work with.
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From most answers - especially Pat's answer - I gather that dehydration is important, so our next test will be whether dehydrating to 90 or 100 % ethanol improves the structure and gets rid of the "bubbles". The alcohol steps were for 30 min each with an overnight break in 70 % alcohol so I do not think longer incubation times will help.
We use LR white because we want to perform immunolabelling, so I think we have to find the right balance between structural preservation and preservation of antigenicity. Our next approach will be with dehydration up to 80, 90, and 100 % ethanol in parallel. Thank you again!
Stefan
==============================Original Headers============================== 4, 30 -- From stefan.wernitznig-at-medunigraz.at Thu Jan 30 07:44:47 2014 4, 30 -- Received: from si068.medunigraz.at (si083.medunigraz.at [193.170.105.83]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0UDikUu024957 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 07:44:47 -0600 4, 30 -- X-AuditID: 0ac80c44-f79eb6d000000e72-fc-52ea574c926c 4, 30 -- Received: from si062.medunigraz.at (Unknown_Domain [10.200.18.79]) 4, 30 -- by si068.medunigraz.at (Symantec Mail Security) with SMTP id 52.93.03698.C475AE25; Thu, 30 Jan 2014 14:44:44 +0100 (CET) 4, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at [10.200.112.128]) 4, 30 -- by si062.medunigraz.at with ESMTP (TLS encrypted); Thu, 30 Jan 2014 14:44:41 +0100 4, 30 -- Message-ID: {52EA574A.3080400-at-medunigraz.at} 4, 30 -- Date: Thu, 30 Jan 2014 14:44:42 +0100 4, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} 4, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 4, 30 -- MIME-Version: 1.0 4, 30 -- To: Microscopy-at-microscopy.com 4, 30 -- Subject: Re: Strange Bubbles in LR white resin 4, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 4, 30 -- Content-Transfer-Encoding: 7bit 4, 30 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFlrJIsWRmVeSWpSXmKPExsXCdULIX9cn/FWQwa4ZEhZ/DjewOzB6fOx+ 4, 30 -- zRTAGMVlk5Kak1mWWqRvl8CV0fNrGnvBWtaKFSfvsTcwzmPpYuTkkBAwkVh/uwnKFpO4cG89 4, 30 -- G4gtJLCXUWJXK18XIxeQPY9RYuvkX0AJDg5eAR2JeQ9YQEwWAVWJk4vzQMrZBCwljnf/ABsj 4, 30 -- KhAssfvxM1YQm1dAUOLkzCdgcREBWYkF74+wg9jCAnoSZ+btZwSxmQVsJP6eWMQCYctLbH87 4, 30 -- h3kCI+8sJO2zkJTNQlK2gJF5FaNwcaaBmYVebmpKaV5melFilV5iySZGcMjwuOxgbNhieIhR 4, 30 -- gINRiYdXwPtVkBBrYllxZe4hRkkOJiVR3sRgoBBfUn5KZUZicUZ8UWlOavEhRgkOZiURXg1N 4, 30 -- oBxvSmJlVWpRPkxKmoNFSZy3IupZkJBAemJJanZqakFqEUxWhoNDSYL3YyhQo2BRanpqRVpm 4, 30 -- TglCmomDE2Q4D9DwhyA1vMUFibnFmekQ+VOMklLivIZhQAkBkERGaR5c7ytGcaAXhHn/g7Tx 4, 30 -- AOMfrusV0EAmoIFiO16ADCxJREhJNTCeWTtjpsiU1S/3ruaOSZ99THeXIS/f4m/ueqLPM1bJ 4, 30 -- rq6WzIkUZdluf4JLSHRBYRZTsNbN976e8yc4/oh/k8NgGbfRZFP71N9V0n6PBEQ117EfcLKq 4, 30 -- kWU0XLVOh5vhrBH3xaBzSrP45HgOu5v3nNR51XhkhlGGWmFxZ3ZJmipv2rSFlqm2jEosxRmJ 4, 30 -- hlrMRcWJAI9kinq8AgAA ==============================End of - Headers==============================
O.k., higher ups at the University have been looking at service contracts costs and trying to
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
==============================Original Headers============================== 4, 24 -- From Edelmare-at-MiamiOH.edu Thu Jan 30 08:44:41 2014 4, 24 -- Received: from mualmaip02.mcs.muohio.edu (mualmaip02.mcs.muohio.edu [134.53.6.8]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0UEieXI012964 4, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 08:44:41 -0600 4, 24 -- X-DNSBL-MILTER: Skipped 4, 24 -- Received: from [10.34.160.232] ([10.34.160.232]) 4, 24 -- by mualmaip02.mcs.muohio.edu (8.14.4/8.14.4) with ESMTP id s0UEiVIV028748 4, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 09:44:31 -0500 4, 24 -- From: "Richard E. Edelmann" {Edelmare-at-MiamiOH.edu} 4, 24 -- To: Microscopy-at-microscopy.com 4, 24 -- Date: Thu, 30 Jan 2014 09:44:32 -0500 4, 24 -- MIME-Version: 1.0 4, 24 -- Subject: U 4, 24 -- Message-ID: {52EA6550.24426.3C0F31C-at-Edelmare.MiamiOH.edu} 4, 24 -- Priority: normal 4, 24 -- X-mailer: Pegasus Mail for Windows (4.63) 4, 24 -- Content-type: text/plain; charset=US-ASCII 4, 24 -- Content-transfer-encoding: 7BIT 4, 24 -- Content-description: Mail message body 4, 24 -- X-Miami-MailScanner-Information: Please contact the ISP for more information 4, 24 -- X-Miami-MailScanner-ID: s0UEiVIV028748 4, 24 -- X-Miami-MailScanner: Found to be clean 4, 24 -- X-Miami-MailScanner-From: edelmare-at-miamioh.edu 4, 24 -- X-Spam-Status: No ==============================End of - Headers==============================
O.k., higher ups at the University have been looking at service contracts costs and trying to find cost reductions. One the vendors they are now thinking about is Unity Asset Management Service (Part of Thermo Fisher Scientific). To provide repair services for all the equipment on campus.
I did a search of the listserv archives and did not find any thing about Unity. SO does anyone out there have any feed back with regards to "Unity Asset Management Service"? For Microscopes or anything else.
I do not want to start a war, and I know alternatoves have been disscussed before, but its new year and time to discuss a little again.
Happy to take anything offline or through the list or even a phone call.
Thank you.
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
==============================Original Headers============================== 9, 24 -- From Edelmare-at-MiamiOH.edu Thu Jan 30 08:53:41 2014 9, 24 -- Received: from mualmaip02.mcs.muohio.edu (mualmaip02.mcs.muohio.edu [134.53.6.8]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0UEreNi031747 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 08:53:41 -0600 9, 24 -- X-DNSBL-MILTER: Skipped 9, 24 -- Received: from [10.34.160.232] ([10.34.160.232]) 9, 24 -- by mualmaip02.mcs.muohio.edu (8.14.4/8.14.4) with ESMTP id s0UErZsv030414 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 09:53:35 -0500 9, 24 -- From: "Richard E. Edelmann" {Edelmare-at-MiamiOH.edu} 9, 24 -- To: Microscopy-at-microscopy.com 9, 24 -- Date: Thu, 30 Jan 2014 09:53:35 -0500 9, 24 -- MIME-Version: 1.0 9, 24 -- Message-ID: {52EA676F.27167.3C93E86-at-Edelmare.MiamiOH.edu} 9, 24 -- Priority: normal 9, 24 -- X-mailer: Pegasus Mail for Windows (4.63) 9, 24 -- Content-type: text/plain; charset=US-ASCII 9, 24 -- Content-transfer-encoding: 7BIT 9, 24 -- Content-description: Mail message body 9, 24 -- X-Miami-MailScanner-Information: Please contact the ISP for more information 9, 24 -- X-Miami-MailScanner-ID: s0UErZsv030414 9, 24 -- X-Miami-MailScanner: Found to be clean 9, 24 -- X-Miami-MailScanner-From: edelmare-at-miamioh.edu 9, 24 -- Subject: Unity Asset Management Service? 9, 24 -- X-Spam-Status: No ==============================End of - Headers==============================
I hope all is well. If you are interested in advancing your EELS and EFTEM (analytical TEM) knowledge, Gatan is offering a 4 day professional training school on April 8-11, 2014 at its R&D Headquarters in Pleasanton, CA, USA. Here are all the details and online registration: http://www.gatan.com/resources/training/EELSSchoolApr2014.php
-- Best regards,
John Marketing Communications Manager
Gatan, Inc.
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==============================Original Headers============================== 9, 25 -- From JHyun-at-gatan.com Thu Jan 30 12:39:43 2014 9, 25 -- Received: from email.gatan.com (email.gatan.com [50.58.128.3]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0UIdg3F022275 9, 25 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 12:39:43 -0600 9, 25 -- Received: from MSE.gatan.com ([fe80::4968:b1cf:a686:47eb]) by email.gatan.com 9, 25 -- ([fe80::5125:aff1:b4a6:ad6e%10]) with mapi id 14.03.0174.001; Thu, 30 Jan 9, 25 -- 2014 10:39:38 -0800 9, 25 -- From: John Hyun {JHyun-at-gatan.com} 9, 25 -- To: Microscopy Server {microscopy-at-microscopy.com} 9, 25 -- Subject: EELS and EFTEM Training School 9, 25 -- Thread-Topic: EELS and EFTEM Training School 9, 25 -- Thread-Index: AQHPHeqgzKsqd/NQX0CXFEwiVH4AOw== 9, 25 -- Date: Thu, 30 Jan 2014 18:39:38 +0000 9, 25 -- Message-ID: {CF0FDB05.2F1FA%jhyun-at-gatan.com} 9, 25 -- In-Reply-To: {201401301351.s0UDpWMJ001473-at-ns.microscopy.com} 9, 25 -- Accept-Language: en-US 9, 25 -- Content-Language: en-US 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- x-originating-ip: [10.13.7.151] 9, 25 -- Content-Type: text/plain; charset="us-ascii" 9, 25 -- Content-ID: {581B1B46E3369F4D9DD2F76175E370C7-at-gatan.com} 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0UIdg3F022275 ==============================End of - Headers==============================
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dear Rick, dear all,
} O.k., higher ups at the University have been looking at service } contracts costs and trying to find cost reductions.
every year the same issue, in your institution and in many/most others as well.
from my 23 years experience, a service contract over 23 years is far better for the scopes and for all users of the scopes (i.e. for all projects!) than 23 years of waiting for the engineer(s), not knowing when (s)he will come, and who is going to pay for it. - We all know this, and most people in the community will agree - except some / many of the "Ups at the University" (nice word...), and those in the Administration.
} One the vendors they are now thinking about is Unity Asset Management } Service (Part of Thermo Fisher Scientific). To provide repair services for } all the equipment on campus.
for most of us: the service engineers of the companies are best trained on the scopes of each particular brand. Thus, a service contract for an electron microscope, and for most of the modern complex CLSM's and their variants, is "best-value-for-money", no doubt about this - in most cases. I am aware that there are other meanings around, other experiences; like: no good experience with the engineer, or too-few-engineers ... (happened to me as well, several times), or trained engineers are available / employed in the institution / University: Not here.
} I did a search of the listserv archives and did not find any thing about Unity. } SO does anyone out there have any feed back with regards to "Unity Asset } Management Service"? For Microscopes or anything else. I do not know anything about Unity, sorry.
One of my arguments. Let's take an (ordinary) car for 25.000 EUR or $ (not really comparible to our scopes, in complexity and pricing!) which is used daily, say 6 hours per day. Quickly, you will end up with an annual mileage of 20.000 km at least, if not 50.000 km (ask your service engineer, who really drive a lot!). This will raise maintenance costs for this car (oil, tyres, repairs, ...) of at least 1.000 EUR or $ per year (i.e. 4% of the initial investment), most likely even higher. Not in the first two years, but then, it will increase, quickly, dramatically. We are prepared to pay for this, because a car is what we think we need, daily. - Our students / postdocs need the scopes daily, probably more than the car (most likely), and in many institutions, the scope is far more complex and used for more hours than only 6 per day. - But, 3 to 4% of the investment for the annual maintenance contract, offering quick and expert response: this is too high, too much money? - And, compare this with the vendor of your car ... are the service engineers as good as those for the scopes? do they travel such long distances, each week / month? Richard, I am facing the same questions, every year or every second year. I still hope that we can convince the people "Up-in-the-Univ" or in the Administration.
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conference: http://www.imc2014.com/ 18th IMC 2014 in Prague, Czech Rep.
==============================Original Headers============================== 11, 27 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Fri Jan 31 02:33:50 2014 11, 27 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0V8XoI4003960 11, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 02:33:50 -0600 11, 27 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 11, 27 -- by localhost (Postfix) with SMTP id 6473A36BC5 11, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 09:33:45 +0100 (CET) 11, 27 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 11, 27 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 11, 27 -- (Client CN "gwsmtp.uni-regensburg.de", Issuer "Uni Regensburg CA - G01" (not verified)) 11, 27 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTPS id 173CF368E8 11, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 09:33:44 +0100 (CET) 11, 27 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 11, 27 -- with Novell_GroupWise; Fri, 31 Jan 2014 09:32:15 +0100 11, 27 -- Message-Id: {52EB6DF70200005400043751-at-gwsmtp1.uni-regensburg.de} 11, 27 -- X-Mailer: Novell GroupWise Internet Agent 12.0.2 11, 27 -- Date: Fri, 31 Jan 2014 09:33:43 +0100 11, 27 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 11, 27 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 11, 27 -- Subject: Unity Asset Management Service? 11, 27 -- References: {201401301453.s0UErpYo032025-at-ns.microscopy.com} 11, 27 -- In-Reply-To: {201401301453.s0UErpYo032025-at-ns.microscopy.com} 11, 27 -- Mime-Version: 1.0 11, 27 -- Content-Type: text/plain; charset=US-ASCII 11, 27 -- Content-Disposition: inline 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0V8XoI4003960 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fxwatanabe-at-ualr.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Center for Integrative Nanotechnology Sciences UALR
Title-Subject: [Filtered] Follow up on Consolidated Service Contract inquiry
Message: Hello everyone who are concerned with similar problem of pressure to consider a consolidated service contract.
There was a post by Richard Edelmann on Unity Asset Management Service. We are being urged to consider another service called Remi (state of Arkansas has a contract with them, I've been told):
https://www.theremigroup.com/default.aspx
Our main instruments are from JEOL (TEM/SEM), Bruker (XRD and AFM), Horiba (Raman, Photoluminescence, Elipsometry), Thermo Fisher (XPS), etc. Does anybody have experience with Remi service (especially with cases involving other vendors such as JEOL, Bruker-AXS, or Thermo Fisher)? I have contacted Richard and have received reply from him already on Unity (thank you very much Richard for very quick reply). Any information on Remi would help us since we have no idea about them.
Thank you very much.
Fumiya
Fumiya Watanabe, Ph.D. Director of Instrumentation Center for Integrative Nanotechnology Sciences ETAS 153E University of Arkansas at Little Rock 2801 S. University Ave. Little Rock, AR 72204 (501) 683-7479 (Office)
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Email: pete.finger-at-jax.org Name: Pete Finger
Organization: The Jackson Laboratory
Title-Subject: [Filtered] Negative Staining Problem
Message: I’ve been doing some negative staining and am experiencing some problems with the Formvar film falling apart on the grid. I’ve been using 200 mesh Copper Formvar/carbon coated grids which are a couple of years old – does anyone know if these grids have a certain shelf life? My technique has been to put a drop of the protein (in HEPES buffer) on the grid, blot dry, add a drop of uranyl acetate, blot dry and then let the grid air dry. When I view the grid on the TEM, pretty much all the Formvar is gone. I haven’t done a lot of negative staining in the past so any comments/suggestions would be greatly appreciated. Thanks much.
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==============================Original Headers============================== 11, 39 -- From nestor.zaluzec-at-gmail.com Fri Jan 31 13:06:52 2014 11, 39 -- Received: from mail-pa0-f47.google.com (mail-pa0-f47.google.com [209.85.220.47]) 11, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0VJ6q1J031041 11, 39 -- for {microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 13:06:52 -0600 11, 39 -- Received: by mail-pa0-f47.google.com with SMTP id kp14so4741577pab.6 11, 39 -- for {microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 11:06:51 -0800 (PST) 11, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 39 -- d=gmail.com; s=20120113; 11, 39 -- h=sender:message-id:date:from:reply-to:user-agent:mime-version:to 11, 39 -- :subject:references:in-reply-to:content-type 11, 39 -- :content-transfer-encoding; 11, 39 -- bh=/1o0BsXPlPcweNFouHZkzKJwGf3uN22ockP2VEwj1Yg=; 11, 39 -- b=giJT+iec0Htv/A7sUkZQO4nd4UbCRGv0IcFHgLAaz1SDH5hibTITR0c6MzX87F4tnS 11, 39 -- cqJX4aHpjAn65/3vtPyxodhC/FrcN1VH636lUms2A2bcztwERBWWXhVtzvedM3z8G9vU 11, 39 -- ++ummWJBip5BDn7tuc6rnziRtWT7mn2ITvc4jhzE4EwQ3yZ6Cu9bCxkXY9rrY541Us61 11, 39 -- HvfFdKT9InjTytZd4kVc2fu5QFI06Xr4DottK9ARBH3zV2z0D15n4ICfJkk0VRKh1X85 11, 39 -- klIL58SaGdyXGnwRV7tFrdu5THm39WlBogTpnUNNtaPn9PaYL9nAeUZf94h7kWMhEKSa 11, 39 -- Ks3g== 11, 39 -- X-Received: by 10.67.5.233 with SMTP id cp9mr22084816pad.147.1391195211735; 11, 39 -- Fri, 31 Jan 2014 11:06:51 -0800 (PST) 11, 39 -- Received: from NestorPwrBk.local ([210.9.11.226]) 11, 39 -- by mx.google.com with ESMTPSA id c7sm30091284pbt.0.2014.01.31.11.06.50 11, 39 -- for {microscopy-at-microscopy.com} 11, 39 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 11, 39 -- Fri, 31 Jan 2014 11:06:51 -0800 (PST) 11, 39 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 11, 39 -- Message-ID: {52EBF446.6040305-at-microscopy.com} 11, 39 -- Date: Fri, 31 Jan 2014 13:06:46 -0600 11, 39 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 11, 39 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 39 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 11, 39 -- MIME-Version: 1.0 11, 39 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 39 -- Subject: viaWWW:Negative Staining Problem 11, 39 -- References: {201401301419.s0UEJrkl012493-at-ns.microscopy.com} 11, 39 -- In-Reply-To: {201401301419.s0UEJrkl012493-at-ns.microscopy.com} 11, 39 -- X-Forwarded-Message-Id: {201401301419.s0UEJrkl012493-at-ns.microscopy.com} 11, 39 -- Content-Type: text/plain; charset=UTF-8; format=flowed 11, 39 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From canadian_drugs15-at-mgts.by Fri Jan 31 14:43:34 2014 Return-Path: {canadian_drugs15-at-mgts.by} Received: from mm-241-186-84-93.dynamic.pppoe.mgts.by ([178.126.52.235]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0VKhRN9004495 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 31 Jan 2014 14:43:28 -0600 Message-Id: {201401312043.s0VKhRN9004495-at-ns.microscopy.com}
Dear all, dear Nestor, dear Fumiya,
in 2012 there was a thread on MSA-Listserver concerning the matter you are requesting:
Thread started 08.05.2012 - ended: 09.06.2012 "Remi Group as a equipmt service contract provider"
There have been several answers. For your convenience (so you would not need to got through the MSA-Listserver Archives):
Since I was interested too, I have collected these Replies in a word.docx/pdf, which I could provide easily in sending to your e-mail-box, if you allow this. Just write to me at w.muss-at-salk.at and receive - with your permission the pdf of this collection of replies.
Best wishes and regards,
Wolfgang
Wolfgang MUSS PhD EM-Lab Univ. Inst. Pathology, SALK-LKH(Gen. Hospital) & PMU (Private Paracelsus Medical University) Salzburg SALZBURG - AUSTRIA
Von: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] Gesendet: Freitag, 31. Jänner 2014 20:11 An: Muß Wolfgang Betreff: [Microscopy] Re(Fwd): Unity Asset Management Service? [by companies, service contracts costs and trying to find cost reductions] // REMI-Service: Follow up on Consolidated Service Contract inquiry
[Microscopy] !! Re: Unity Asset Management Service? [by companies, service contracts costs and trying to find cost reductions] !! // Follow up on Consolidated Service Contract inquiry
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Hello everyone who are concerned with similar problem of pressure to consider a consolidated service contract.
There was a post by Richard Edelmann on Unity Asset Management Service. We are being urged to consider another service called Remi (state of Arkansas has a contract with them, I've been told):
https://www.theremigroup.com/default.aspx
Our main instruments are from JEOL (TEM/SEM), Bruker (XRD and AFM), Horiba (Raman, Photoluminescence, Elipsometry), Thermo Fisher (XPS), etc. Does anybody have experience with Remi service (especially with cases involving other vendors such as JEOL, Bruker-AXS, or Thermo Fisher)?
I have contacted Richard and have received reply from him already on Unity (thank you very much Richard for very quick reply). Any information on Remi would help us since we have no idea about them.
Thank you very much.
Fumiya
Fumiya Watanabe, Ph.D. Director of Instrumentation Center for Integrative Nanotechnology Sciences ETAS 153E University of Arkansas at Little Rock 2801 S. University Ave. Little Rock, AR 72204 (501) 683-7479 (Office)
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==============================Original Headers============================== 29, 40 -- From W.Muss-at-salk.at Mon Feb 3 09:34:30 2014 29, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 29, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s13FYTZV001453 29, 40 -- for {microscopy-at-microscopy.com} ; Mon, 3 Feb 2014 09:34:29 -0600 29, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 29, 40 -- by hermes.salk.at (Postfix) with ESMTP id 1A707646B2D 29, 40 -- for {microscopy-at-microscopy.com} ; Mon, 3 Feb 2014 16:34:28 +0100 (CET) 29, 40 -- X-Scanned-By: SALK-Content-Filter 29, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 29, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 29, 40 -- with ESMTP id 34aRAcCrjRRD for {microscopy-at-microscopy.com} ; 29, 40 -- Mon, 3 Feb 2014 16:34:27 +0100 (CET) 29, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 29, 40 -- by hermes.salk.at (Postfix) with ESMTP id CF9EE646B40 29, 40 -- for {microscopy-at-microscopy.com} ; Mon, 3 Feb 2014 16:34:27 +0100 (CET) 29, 40 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 29, 40 -- by N1EX198.lks.local with ESMTP; 03 Feb 2014 16:39:41 +0100 29, 40 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 29, 40 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 29, 40 -- 14.02.0387.000; Mon, 3 Feb 2014 16:34:27 +0100 29, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 29, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 29, 40 -- Subject: [Microscopy] Re(Fwd): Unity Asset Management Service? // 29, 40 -- REMI-Service: Follow up on Consolidated Service Contract inquiry 29, 40 -- Thread-Topic: [Microscopy] Re(Fwd): Unity Asset Management Service? // 29, 40 -- REMI-Service: Follow up on Consolidated Service Contract inquiry 29, 40 -- Thread-Index: AQHPIPVreSPfIxDLmEK+2UCpUnWrsw== 29, 40 -- Date: Mon, 3 Feb 2014 15:34:26 +0000 29, 40 -- Message-ID: {3D22E6EE65F39E448DCE4162815376711C57BF30-at-N2DT131.lksdom21.lks.local} 29, 40 -- References: {201401311910.s0VJAYg5006429-at-ns.microscopy.com} 29, 40 -- In-Reply-To: {201401311910.s0VJAYg5006429-at-ns.microscopy.com} 29, 40 -- Accept-Language: de-DE, en-US 29, 40 -- Content-Language: de-DE 29, 40 -- X-MS-Has-Attach: 29, 40 -- X-MS-TNEF-Correlator: 29, 40 -- x-originating-ip: [192.168.42.2] 29, 40 -- Content-Type: text/plain; charset="iso-8859-1" 29, 40 -- MIME-Version: 1.0 29, 40 -- Content-Transfer-Encoding: 8bit 29, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s13FYTZV001453 ==============================End of - Headers==============================
From boakkeau-at-wegl.net Mon Feb 3 09:59:44 2014 Return-Path: {boakkeau-at-wegl.net} Received: from mail3.wegl.net ([178.126.142.44]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s13FxfSr020679 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 3 Feb 2014 09:59:42 -0600 Message-Id: {201402031559.s13FxfSr020679-at-ns.microscopy.com}
Pete,
Films on grids do tend to break when old. I used some 75 mesh grids that were 5 years old recently and they were still good but I'd not try to use single holed ones that were that old unless I really needed to. With older 200 mesh grids one can usually get some areas that do not pop but use newer ones if you can afford them or make them yourself. Perhaps your drying step is a bit harsh. The word "blot" is what caught my eye. It is hard to judge unless it could be observed.
I was taught to do my negative staining differently than how you describe. I put a sample onto a recently glow discharged grid held in forcepts, let it sit for 30 to 60 seconds, gently drop 5-6 drops of 1%UA from a pipet onto the surface of the grid held perpendicular to the grid (grid parallel to the table top over a vessel to catch the UA). After the last drop of UA is on the grid I touch a point of filter paper to the junction of the forcepts where the grid is being held to slowly wick the UA off. The surface of the grid will still be wet. There is usually a small amount of UA between the forcept tips so I "push" the grid out of the forcepts with the same piece of filter paper using another portion that is not already wet, onto some lens tissue to continue to dry completely.
There are many different techniques in doing negative staining. Once you find one that works for you that is the one you should stick with.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Pat
Patricia Stranen Connelly NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491
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Email: pete.finger-at-jax.org Name: Pete Finger
Organization: The Jackson Laboratory
Title-Subject: [Filtered] Negative Staining Problem
Message: I?ve been doing some negative staining and am experiencing some problems with the Formvar film falling apart on the grid. I?ve been using 200 mesh Copper Formvar/carbon coated grids which are a couple of years old ? does anyone know if these grids have a certain shelf life? My technique has been to put a drop of the protein (in HEPES buffer) on the grid, blot dry, add a drop of uranyl acetate, blot dry and then let the grid air dry. When I view the grid on the TEM, pretty much all the Formvar is gone. I haven?t done a lot of negative staining in the past so any comments/suggestions would be greatly appreciated. Thanks much.
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==============================Original Headers============================== 21, 29 -- From connellyps-at-nhlbi.nih.gov Mon Feb 3 11:38:29 2014 21, 29 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s13HcQuu022068 21, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Feb 2014 11:38:28 -0600 21, 29 -- X-IronPortListener: Outbound_SMTP 21, 29 -- X-IronPort-Anti-Spam-Filtered: true 21, 29 -- X-IronPort-Anti-Spam-Result: AiUFABHT71KcKEeo/2dsb2JhbABWA4MMOFeDAbsIGHQWdIIlAQIEEiIaPQEVCgUICgEVBB0SAR0KBBMZBAQBh2MNkAmMYIR0ihEOoUSBII0HEQFdCwEBglOBUgSLU4MRiUaBMoU+izGDLYFqBxci 21, 29 -- Received: from unknown (HELO CASHTV04.nih.gov) ([156.40.71.168]) 21, 29 -- by nihxway2out.hub.nih.gov with ESMTP/TLS/AES128-SHA; 03 Feb 2014 12:38:25 -0500 21, 29 -- Received: from MLBXV14.nih.gov ([169.254.4.96]) by CASHTV04.nih.gov 21, 29 -- ([156.40.71.168]) with mapi id 14.03.0174.001; Mon, 3 Feb 2014 12:38:25 -0500 21, 29 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 21, 29 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 21, 29 -- Subject: Negative Staining Problem 21, 29 -- Thread-Topic: Negative Staining Problem 21, 29 -- Thread-Index: AQHPIQa9M4ubQaW/WkOnMozQy1n7dg== 21, 29 -- Date: Mon, 3 Feb 2014 17:38:25 +0000 21, 29 -- Message-ID: {CF153E40.12FD%connellyps-at-mail.nih.gov} 21, 29 -- Accept-Language: en-US 21, 29 -- Content-Language: en-US 21, 29 -- X-MS-Has-Attach: 21, 29 -- X-MS-TNEF-Correlator: 21, 29 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 21, 29 -- x-originating-ip: [156.40.71.189] 21, 29 -- Content-Type: text/plain; charset="big5" 21, 29 -- Content-ID: {6C33295EF427DB459068692462DC21D1-at-mail.nih.gov} 21, 29 -- MIME-Version: 1.0 21, 29 -- Content-Transfer-Encoding: 8bit 21, 29 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s13HcQuu022068 ==============================End of - Headers==============================
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Email: tjzhang2000-at-yahoo.com Name: Tim Zhang
Organization: GA Tech
Title-Subject: [Filtered] 200KV used TEM wanted
Message: Dear All,
We are looking for am used TEM, 200-300KV not FEG gun. If you know any info about it please let me know.
Thank you very much for your time and help.
Tim
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Email: 12hy1-at-queensu.ca Name: Hongbing Yu
Organization: Queens' University, Canada
Title-Subject: [Filtered] EDS can't detect tin
Message: We are trying to use FEI TECNAI ORISIS TEM characterize the element distribution of a Zirconium alloy with 3.5 atom% Sn. However we can't detect tin anywhere with EDS in STEM Mode with beam spot 9. The EDS system works very well for a ODS steel. We have tried our best to figure out this problem, but we failed. Any comment and suggestion would be appreciated.
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Email: slc6-at-lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh Microscopy School 2014
Message: There is still time to register for the 2014 Lehigh Microscopy School which will be held June 8-14, 2014. ALL courses will be offered in the same week which means that ALL lecturers and ALL instrument suppliers will be together for what promises to be a phenomenal week. This year will be the 44th anniversary for the Lehigh Microscopy School! Course offerings include: Scanning Electron Microscopy and X-ray Microanalysis • Introduction to SEM and EDS for the New Operator • Focused Ion Beam Instrumentation and Applications • Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD Data • Quantitative X-ray Microanalysis: Problem Solving Using EDS and WDS Techniques • Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register by April 15 for an Early Bird Discount! Contact Sharon Coe (sharon.coe-at-lehigh.edu or 610-758-5133). www.lehigh.edu/microscopy
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This may sound like a silly suggestion but if you haven't already, I would try the following things:
1) Use an enormous beam in TEM mode to look around on micron-scale and look for Sn in EDS all over any thin area. You may just be looking at an unusual part of the specimen where the Sn is gone and not know it.
2) If you can do EELS, check for Sn there - should be an M edge about 500eV I think (look in EELS Atlas, I am being vague). If you cannot see Sn anywhere by EELS or EDS then the problem is not the EDS detector, the problem is that your specimen doesn't have Sn in it, and you now have to figure out why. (Preferential removal of Sn during sample prep? Something happened when making the material originally?)
Good luck!
Yours
Jo Sharp
On 4 February 2014 21:16, {zaluzec-at-microscopy.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: 12hy1-at-queensu.ca () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: 12hy1-at-queensu.ca } Name: Hongbing Yu } } Organization: Queens' University, Canada } } Title-Subject: [Filtered] EDS can't detect tin } } Message: We are trying to use FEI TECNAI ORISIS TEM characterize the } element distribution of a Zirconium alloy with 3.5 atom% Sn. However we } can't detect tin anywhere with EDS in STEM Mode with beam spot 9. The } EDS system works very well for a ODS steel. We have tried our best to } figure out this problem, but we failed. Any comment and suggestion would } be appreciated. } } Login Host: 130.15.116.215 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } -- } =============================================== } } Do not reply to this messages it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================= } } ==============================Original Headers============================== } 14, 39 -- From nestor.zaluzec-at-gmail.com Tue Feb 4 15:01:57 2014 } 14, 39 -- Received: from mail-pb0-f52.google.com (mail-pb0-f52.google.com [209.85.160.52]) } 14, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s14L1uOP006323 } 14, 39 -- for {microscopy-at-microscopy.com} ; Tue, 4 Feb 2014 15:01:57 -0600 } 14, 39 -- Received: by mail-pb0-f52.google.com with SMTP id jt11so8922418pbb.25 } 14, 39 -- for {microscopy-at-microscopy.com} ; Tue, 04 Feb 2014 13:01:56 -0800 (PST) } 14, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 39 -- d=gmail.com; s=20120113; } 14, 39 -- h=sender:message-id:date:from:reply-to:user-agent:mime-version:to } 14, 39 -- :subject:references:in-reply-to:content-type } 14, 39 -- :content-transfer-encoding; } 14, 39 -- bh=klHeYixvGJoedxJSpxTWqT+OgUGT4cK30LG0Velz21c=; } 14, 39 -- b=VHelNtSKe4o6QEI7zC8SiSpg+d6O7gCFA5BJI2X2uU7p7TMZjShyCgixgeYbGPeETV } 14, 39 -- jUOtDTuX5RrTZNezNYlaJvmTlKbVQGW8J2FmJonM/kfjPDqyE8sEoMSS+gl9Fc1TDjVY } 14, 39 -- LRrrK+BDobBp7uUAHQoE/HHOMlH6KGCWOwLyjD/gE9wlAkP8Fx6CZdiQYjWdR74DG2iL } 14, 39 -- wmzcxyzi4pxXw1d0S0o4rhltjFldrMeltxpLgfi9C0OOwewSfwn2Cp6X/MzOg+l5PRTf } 14, 39 -- Wr2wgfmYuxeVQNVYsn8ZWdErsfLXRFTKHJ/PdlNwIjNe/lW8YbMaahDkeb0ctNZAi60u } 14, 39 -- UPvw== } 14, 39 -- X-Received: by 10.66.154.169 with SMTP id vp9mr45713120pab.39.1391547716282; } 14, 39 -- Tue, 04 Feb 2014 13:01:56 -0800 (PST) } 14, 39 -- Received: from NestorPwrBk.local ([210.9.11.226]) } 14, 39 -- by mx.google.com with ESMTPSA id nu10sm69172882pbb.16.2014.02.04.13.01.54 } 14, 39 -- for {microscopy-at-microscopy.com} } 14, 39 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); } 14, 39 -- Tue, 04 Feb 2014 13:01:55 -0800 (PST) } 14, 39 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} } 14, 39 -- Message-ID: {52F15540.5030100-at-microscopy.com} } 14, 39 -- Date: Wed, 05 Feb 2014 07:31:52 +1030 } 14, 39 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} } 14, 39 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 14, 39 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 } 14, 39 -- MIME-Version: 1.0 } 14, 39 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 39 -- Subject: viaWWW:EDS can't detect tin } 14, 39 -- References: {201402041501.s14F1HJL030540-at-ns.microscopy.com} } 14, 39 -- In-Reply-To: {201402041501.s14F1HJL030540-at-ns.microscopy.com} } 14, 39 -- X-Forwarded-Message-Id: {201402041501.s14F1HJL030540-at-ns.microscopy.com} } 14, 39 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 14, 39 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 39 -- From j.sharp-at-sheffield.ac.uk Wed Feb 5 06:42:39 2014 8, 39 -- Received: from mailhost1.shef.ac.uk (mailhost1.shef.ac.uk [143.167.251.162]) 8, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s15Cgd18013449 8, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Feb 2014 06:42:39 -0600 8, 39 -- Received: from mail-yk0-f171.google.com ([209.85.160.171]) 8, 39 -- by mailhost1.shef.ac.uk with esmtps (TLSv1:RC4-SHA:128) 8, 39 -- (Exim 4.77) 8, 39 -- (envelope-from {j.sharp-at-sheffield.ac.uk} ) 8, 39 -- id 1WB1oL-00032o-VF 8, 39 -- for Microscopy-at-microscopy.com; Wed, 05 Feb 2014 12:42:38 +0000 8, 39 -- Received: by mail-yk0-f171.google.com with SMTP id 142so784856ykq.2 8, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 05 Feb 2014 04:42:36 -0800 (PST) 8, 39 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 39 -- d=1e100.net; s=20130820; 8, 39 -- h=x-gm-message-state:mime-version:in-reply-to:references:date 8, 39 -- :message-id:subject:from:to:content-type; 8, 39 -- bh=Q3Kfa1KJ8S4ket2YYdu0cCGxK5z3tlo6rSpK1gm7GT0=; 8, 39 -- b=PxtBfmdNr/g9t4OCxISV/xUW3LfyXUceoH5ZxbCmUyyY8WI6wntEooyamUasxIIJjE 8, 39 -- c8w3zXsSWiBDEtmXj7Xgum7YwCI8MIS/wNDIkw5qD6fmoIy9WT9Bp8hoFsYHzpDbCktO 8, 39 -- IGK8/1fTCw/xMfwtDQE6sNVsnU07OvjzQNM5LC/d7jCb6bygUGuWVPVBaRShZ4koFmFn 8, 39 -- 8foKD4w5TLhkD/v+EWTTwfqk99qXz//ull2COcHQYpf0JyiWrivNnmAyppOGL89yYs+i 8, 39 -- U8liwh64CGGbG+nG901eLwbiN+AIrQGQVID5QiIot86Pwtf66AUg0D/nL1X2I+lRv9C2 8, 39 -- Fs+w== 8, 39 -- X-Gm-Message-State: ALoCoQkVAsqF2fwr1rkK/vq33Pw2mNgPV/XlFE8RcPkO1xKZQbx0fztWe3LYtfejRN0mjXnZpW76Yl85PSffxB/Si4j60fxyMM6JxcjnlC0nA7tNGSBOmKqRsI/G5iYOEGngsVu7F12eqIrs4qc7CS+etaCfKfXFZxR6WsV3Y+6RumbLFGXXKLs= 8, 39 -- X-Received: by 10.236.38.74 with SMTP id z50mr154390yha.134.1391604156934; 8, 39 -- Wed, 05 Feb 2014 04:42:36 -0800 (PST) 8, 39 -- MIME-Version: 1.0 8, 39 -- X-Received: by 10.236.38.74 with SMTP id z50mr154380yha.134.1391604156804; 8, 39 -- Wed, 05 Feb 2014 04:42:36 -0800 (PST) 8, 39 -- Received: by 10.170.63.197 with HTTP; Wed, 5 Feb 2014 04:42:36 -0800 (PST) 8, 39 -- In-Reply-To: {201402042116.s14LGSVL032283-at-ns.microscopy.com} 8, 39 -- References: {201402042116.s14LGSVL032283-at-ns.microscopy.com} 8, 39 -- Date: Wed, 5 Feb 2014 12:42:36 +0000 8, 39 -- Message-ID: {CAG8d=kXyexSXXmA3JFNt8mTUU_VoKn=gPycaMMOQ36q1VCsRWg-at-mail.gmail.com} 8, 39 -- Subject: Re: [Microscopy] viaWWW:EDS can't detect tin 8, 39 -- From: Joanne Sharp {j.sharp-at-sheffield.ac.uk} 8, 39 -- To: 12hy1-at-queensu.ca, Microscopy-at-microscopy.com 8, 39 -- Content-Type: text/plain; charset=ISO-8859-1 8, 39 -- X-S0phie-Scan: No ==============================End of - Headers==============================
March 3-7, 2014 a course in polarized light microscopy will be offered at Hooke College of Applied Sciences. Further information about this hands-on course can be found at the link below:
Best regards- __________________________________________________ Chris Gorman Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax) www.hookecollege.com
==============================Original Headers============================== 7, 25 -- From CGorman-at-hookecollege.com Wed Feb 5 11:58:21 2014 7, 25 -- Received: from mail.mccrone.com (mail.mccrone.com [12.54.22.114]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s15HwLCN003704 7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 5 Feb 2014 11:58:21 -0600 7, 25 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by 7, 25 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Wed, 5 Feb 2014 11:58:19 7, 25 -- -0600 7, 25 -- From: Christine Gorman {CGorman-at-hookecollege.com} 7, 25 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 25 -- Date: Wed, 5 Feb 2014 11:58:18 -0600 7, 25 -- Subject: PLM Short Course Announcement: March 3-7, 2014 7, 25 -- Thread-Topic: PLM Short Course Announcement: March 3-7, 2014 7, 25 -- Thread-Index: Ac8im738S+31181IScWvZZxXDySEmQ== 7, 25 -- Message-ID: {874B1DB532886444A60A015EE363493F9D84EB29E6-at-mccronemsg07.tmg.mccrone.com} 7, 25 -- Accept-Language: en-US 7, 25 -- Content-Language: en-US 7, 25 -- X-MS-Has-Attach: 7, 25 -- X-MS-TNEF-Correlator: 7, 25 -- x-ninja-pim: Scanned by Ninja 7, 25 -- x-ninja-attachmentfiltering: (no action) 7, 25 -- acceptlanguage: en-US 7, 25 -- Content-Type: text/plain; charset="us-ascii" 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s15HwLCN003704 ==============================End of - Headers==============================
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Message: MSNO Winter MEETING will be on Wednesday, February 26th, 2014, 4:00 – 8:30 p.m. Dittrick Medical History Center Allen Memorial Medical Library 11000 Euclid Ave. Cleveland, OH 44106-1714
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anybody out there to help me with a service manual and a parts list for the Reichert Ultracut E ultramicrotome? All I have is the user manual.
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
I was just sent some material on a new microscope from Seron and it occurred to me I have not really heard much on the list about this brand. Has anyone tried one or have one even? How do they fair to other entry level micorscopes. Comments on or off list would be appricated.
Thanks John in the UK (Bath Uni)
==============================Original Headers============================== 3, 26 -- From john.mitchels-at-gmail.com Thu Feb 6 07:47:47 2014 3, 26 -- Received: from mail-vb0-f46.google.com (mail-vb0-f46.google.com [209.85.212.46]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s16DlkqV029411 3, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Feb 2014 07:47:46 -0600 3, 26 -- Received: by mail-vb0-f46.google.com with SMTP id o19so1405181vbm.19 3, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 06 Feb 2014 05:47:46 -0800 (PST) 3, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=20120113; 3, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 26 -- bh=WgJjxQNNeV1lxO2rTvgtlydWlgDE+GmL4qAuWaEmUSQ=; 3, 26 -- b=ydBjVMSMrl6ozJn3iXeLDBns4C/0vu8W0ZP1iEw3FlI/J9md6F9tQlAx1t4ItMo+i2 3, 26 -- rcYWaCOi8hmE6ivDp7CasFzYkAdcZbkinkOU0vuaP80r0B5yB8bLtta7JyUTgv7a0s4v 3, 26 -- i4C913ans5M77zpaIFD/Kb1flvOb9j8izKJNIgcxPnP/U3rSBhOXjBJSmpQn6wS/l0UM 3, 26 -- QknMrRo6baTdUGugLCAAH6p8xJl3pRdBBCPP18UHCx4bEMD1MAO293mDi+qpf4cquSE+ 3, 26 -- uLQ9NBggFVC7fE0WqH8krU8NK2G/0IvjN3X/ug344HpM+uhgsvAid0NsRSwXFrhUqfT8 3, 26 -- L3SQ== 3, 26 -- MIME-Version: 1.0 3, 26 -- X-Received: by 10.52.107.35 with SMTP id gz3mr4917851vdb.8.1391694466192; Thu, 3, 26 -- 06 Feb 2014 05:47:46 -0800 (PST) 3, 26 -- Received: by 10.220.165.68 with HTTP; Thu, 6 Feb 2014 05:47:46 -0800 (PST) 3, 26 -- Date: Thu, 6 Feb 2014 13:47:46 +0000 3, 26 -- Message-ID: {CAHX7yTQnjfh+sdvbbLhgnGUS2w74+H21YjM5NWkesVbPJXh87Q-at-mail.gmail.com} 3, 26 -- Subject: Seron SEMs any views? 3, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 3, 26 -- To: Microscopy-at-microscopy.com 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Dear All, thanks, I finally got a PDF of the service manual.
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 06.02.14 14:28, schrieb Muß Wolfgang: } bradford.ross-at-botany.ubc.ca {mailto:bradford.ross-at-botany.ubc.ca} } } Stefan: } } ich bin ja schon sehr froh, wenn ich als „Notnagel“ wenigstens da und dort einmal was „tun“ kann für die Community....Für Dich } allemal von Herzen! } } Habe noch was gefunden (originales pdf hatte 13 MB, daher habe ich es nochmals als pdf „gedruckt“ und damit auf ca. 7MB } komprimiert...das hat etwas gedauert...) } } Die Dokumentation hatte ich dankenswerterweise von bradford.ross-at-botany.ubc.ca {mailto:bradford.ross-at-botany.ubc.ca} im Nov. 2013 } erhalten. } } Ebenfalls „sonnige“, und v. a. {föhnige} Grüße aus Salzburg (dzt. wie im späten April: knallblauer Himmel bei ca. 15Grad C! } } Wolfgang } } *Von:*Stefan Diller [mailto:stefan.diller-at-t-online.de] } *Gesendet:* Donnerstag, 06. Februar 2014 14:21 } *An:* Muß Wolfgang } *Betreff:* Re: persönl. AW(2): [Microscopy] Reichert Ultracut E service manual } } Lieber Wolfgang, } wie immer der Retter in der Not ;-) } } Herzliche Grüße in den Süden, } Stefan } } Am 06.02.2014 14:18, schrieb Muß Wolfgang: } } Lieber Stefan, } } das ist alles, was ich in meinen files gefunden habe.... } } mhG } } und beste Wünsche beim Basteln... } } Wolfgang } } *Von:*stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} [mailto:stefan.diller-at-t-online.de] } *Gesendet:* Donnerstag, 06. Februar 2014 11:02 } *An:* Muß Wolfgang } *Betreff:* [Microscopy] Reichert Ultracut E service manual } } --------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } Dear All, } } anybody out there to help me with a service manual and a parts list for } } the Reichert Ultracut E ultramicrotome? } } All I have is the user manual. } } Thanks, } } Stefan } } -- } } ----------------------------------------------------- } } Stefan Diller - Scientific Photography } } Arndtstrasse 22 } } D - 97072 Wuerzburg Germany } } ++49-931-7848700 Phone } } ++49-931-7848701 Fax } } ++49-175-7177051 Mobile } } Websites: } } www.nanoflight.info {http://www.nanoflight.info} } } www.electronmicroscopy.info {http://www.electronmicroscopy.info} } } www.elektronenmikroskopie.info {http://www.elektronenmikroskopie.info} } } www.assisi.de {http://www.assisi.de} } } www.zwillingsprojekt.de {http://www.zwillingsprojekt.de} } } www.stefan-diller.com {http://www.stefan-diller.com} } } Anfahrt: http://Mail.map24.com/Stefan.Diller } } ----------------------------------------------------- } } ==============================Original Headers============================== } } 9, 20 -- From stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} Thu Feb 6 03:57:17 2014 } } 9, 20 -- Received: from mailout05.t-online.de (mailout05.t-online.de [194.25.134.82]) } } 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s169vG4m007656 } } 9, 20 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 6 Feb 2014 03:57:16 -0600 } } 9, 20 -- Received: from fwd27.aul.t-online.de (fwd27.aul.t-online.de ) } } 9, 20 -- by mailout05.t-online.de with smtp } } 9, 20 -- id 1WBLhp-0003UD-Oc; Thu, 06 Feb 2014 10:57:14 +0100 } } 9, 20 -- Received: from [192.168.2.202] (S90VZgZOgh7NeC2laO0ho+P-GiMlg8xpksETwKZJ07y+8vUsNdUHVcgzJrB2Z5IwK2-at-[91.58.180.49 } {mailto:S90VZgZOgh7NeC2laO0ho+P-GiMlg8xpksETwKZJ07y+8vUsNdUHVcgzJrB2Z5IwK2-at-[91.58.180.49} ]) by fwd27.t-online.de } } 9, 20 -- with esmtp id 1WBLhi-1sCXNA0; Thu, 6 Feb 2014 10:57:06 +0100 } } 9, 20 -- Message-ID: {52F35C74.2020707-at-t-online.de {mailto:52F35C74.2020707-at-t-online.de} } } } 9, 20 -- Date: Thu, 06 Feb 2014 10:57:08 +0100 } } 9, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} } } } 9, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 } } 9, 20 -- MIME-Version: 1.0 } } 9, 20 -- To: microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 9, 20 -- Subject: Reichert Ultracut E service manual } } 9, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } } 9, 20 -- Content-Transfer-Encoding: 7bit } } 9, 20 -- X-ID: S90VZgZOgh7NeC2laO0ho+P-GiMlg8xpksETwKZJ07y+8vUsNdUHVcgzJrB2Z5IwK2 } } 9, 20 -- X-TOI-MSGID: bd40bafa-19ef-4dcc-b708-8ce2056c24bc } } ==============================End of - Headers============================== } } } } -- } } } } } } } } ----------------------------------------------------- } } Stefan Diller - Scientific Photography } } Arndtstrasse 22 } } D - 97072 Wuerzburg Germany } } ++49-931-7848700 Phone } } ++49-931-7848701 Fax } } ++49-175-7177051 Mobile } } } } Websites: } } www.nanoflight.info {http://www.nanoflight.info} } } www.electronmicroscopy.info {http://www.electronmicroscopy.info} } } www.elektronenmikroskopie.info {http://www.elektronenmikroskopie.info} } } www.assisi.de {http://www.assisi.de} } } www.zwillingsprojekt.de {http://www.zwillingsprojekt.de} } } www.stefan-diller.com {http://www.stefan-diller.com} } } Anfahrt:http://Mail.map24.com/Stefan.Diller } } ----------------------------------------------------- }
==============================Original Headers============================== 9, 23 -- From stefan.diller-at-t-online.de Thu Feb 6 08:43:56 2014 9, 23 -- Received: from mailout07.t-online.de (mailout07.t-online.de [194.25.134.83]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s16Eht6u016458 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2014 08:43:55 -0600 9, 23 -- Received: from fwd30.aul.t-online.de (fwd30.aul.t-online.de ) 9, 23 -- by mailout07.t-online.de with smtp 9, 23 -- id 1WBQBF-00056Z-RM; Thu, 06 Feb 2014 15:43:53 +0100 9, 23 -- Received: from mac-pro.speedport.ip (VmpAcqZCrh1txE7m6SOz7MPAk7Om5JjbWiIS7qZ2+4SLtNlgHCNp8xu25vcJumeQMy-at-[84.174.121.68]) by fwd30.t-online.de 9, 23 -- with esmtp id 1WBQBF-0MAdUG0; Thu, 6 Feb 2014 15:43:53 +0100 9, 23 -- Message-ID: {52F39FA9.6030306-at-t-online.de} 9, 23 -- Date: Thu, 06 Feb 2014 15:43:53 +0100 9, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 9, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 9, 23 -- MIME-Version: 1.0 9, 23 -- To: microscopy-at-microscopy.com 9, 23 -- Subject: Re: [Microscopy] Reichert Ultracut E service manual 9, 23 -- References: {201402061002.s16A25H5012082-at-ns.microscopy.com} {3D22E6EE65F39E448DCE4162815376711C58A178-at-N2DT131.lksdom21.lks.local} {52F38C47.8030604-at-t-online.de} {3D22E6EE65F39E448DCE4162815376711C58A77C-at-N2DT131.lksdom21.lks.local} 9, 23 -- In-Reply-To: {3D22E6EE65F39E448DCE4162815376711C58A77C-at-N2DT131.lksdom21.lks.local} 9, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 9, 23 -- X-ID: VmpAcqZCrh1txE7m6SOz7MPAk7Om5JjbWiIS7qZ2+4SLtNlgHCNp8xu25vcJumeQMy 9, 23 -- X-TOI-MSGID: 99d1ef5d-1298-431a-9657-4f288b4ebe1e 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s16Eht6u016458 ==============================End of - Headers==============================
I originally posted this as "free SEM" but Nestor's spam filter threw me under the bus...
A friend of mine passed this ad on to me. I have no connection to the people giving this away, just thought someone out there might be interested. I'm not familiar with the make/model. Anybody? It does appear to be "vintage"...
Hello Microscopists, Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great.
Thanks for your thoughts and answers in advance Alejandro Hinojos
==============================Original Headers============================== 6, 29 -- From ahinojos2-at-miners.utep.edu Thu Feb 6 20:17:12 2014 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172HCnn004797 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Feb 2014 20:17:12 -0600 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com (10.255.210.155) by 6, 29 -- BLUPR05MB006.namprd05.prod.outlook.com (10.255.210.142) with Microsoft SMTP 6, 29 -- Server (TLS) id 15.0.868.8; Fri, 7 Feb 2014 02:17:10 +0000 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.44]) by 6, 29 -- BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.156]) with mapi id 6, 29 -- 15.00.0868.013; Fri, 7 Feb 2014 02:17:10 +0000 6, 29 -- From: "Hinojos, Alejandro" {ahinojos2-at-miners.utep.edu} 6, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 6, 29 -- Subject: TEM Acquisition Help 6, 29 -- Thread-Topic: TEM Acquisition Help 6, 29 -- Thread-Index: AQHPI6qIDXb0k8Img0O3WLndhK0Dmg== 6, 29 -- Date: Fri, 7 Feb 2014 02:17:10 +0000 6, 29 -- Message-ID: {1e44fc83cbc14812ac71282723f64e06-at-BLUPR05MB008.namprd05.prod.outlook.com} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- x-originating-ip: [129.108.37.93] 6, 29 -- x-forefront-prvs: 011579F31F 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(199002)(189002)(74876001)(53806001)(90146001)(76482001)(31966008)(74706001)(76796001)(56816005)(76176001)(75432001)(54316002)(92566001)(81816001)(85852003)(87936001)(63696002)(46102001)(76576001)(76786001)(77096001)(51856001)(54356001)(66066001)(65816001)(74502001)(81686001)(74662001)(47446002)(47976001)(2656002)(83322001)(56776001)(81342001)(94946001)(94316002)(87266001)(33646001)(81542001)(47736001)(83072002)(93516002)(74316001)(86362001)(74366001)(50986001)(93136001)(80022001)(69226001)(79102001)(4396001)(59766001)(80976001)(85306002)(49866001)(95416001)(77982001)(24736002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR05MB006;H:BLUPR05MB008.namprd05.prod.outlook.com;CLIP:129.108.37.93;FPR:3ADEF03E.9E339C09.87D2589.84EF8869.201A3;InfoNoRecordsA:0;MX:1;LANG:en; 6, 29 -- Content-Type: text/plain; charset="Windows-1252" 6, 29 -- MIME-Version: 1.0 6, 29 -- X-OriginatorOrg: miners.utep.edu 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172HCnn004797 ==============================End of - Headers==============================
It is a 5kV instrument. For TEM (or STEM), ask yourself what thickness you can look through with a 5kV beam.
Cheers, Henk
On 2/6/2014 9:19 PM, ahinojos2-at-miners.utep.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hello Microscopists, } Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great. } } } Thanks for your thoughts and answers in advance } Alejandro Hinojos } } } } } ==============================Original Headers============================== } 6, 29 -- From ahinojos2-at-miners.utep.edu Thu Feb 6 20:17:12 2014 } 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) } 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172HCnn004797 } 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Feb 2014 20:17:12 -0600 } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com (10.255.210.155) by } 6, 29 -- BLUPR05MB006.namprd05.prod.outlook.com (10.255.210.142) with Microsoft SMTP } 6, 29 -- Server (TLS) id 15.0.868.8; Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.44]) by } 6, 29 -- BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.156]) with mapi id } 6, 29 -- 15.00.0868.013; Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- From: "Hinojos, Alejandro" {ahinojos2-at-miners.utep.edu} } 6, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 6, 29 -- Subject: TEM Acquisition Help } 6, 29 -- Thread-Topic: TEM Acquisition Help } 6, 29 -- Thread-Index: AQHPI6qIDXb0k8Img0O3WLndhK0Dmg== } 6, 29 -- Date: Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- Message-ID: {1e44fc83cbc14812ac71282723f64e06-at-BLUPR05MB008.namprd05.prod.outlook.com} } 6, 29 -- Accept-Language: en-US } 6, 29 -- Content-Language: en-US } 6, 29 -- X-MS-Has-Attach: } 6, 29 -- X-MS-TNEF-Correlator: } 6, 29 -- x-originating-ip: [129.108.37.93] } 6, 29 -- x-forefront-prvs: 011579F31F } 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(199002)(189002)(74876001)(53806001)(90146001)(76482001)(31966008)(74706001)(76796001)(56816005)(76176001)(75432001)(54316002)(92566001)(81816001)(85852003)(87936001)(63696002)(46102001)(76576001)(76786001)(77096001)(51856001)(54356001)(66066001)(65816001)(74502001)(81686001)(74662001)(47446002)(47976001)(2656002)(83322001)(56776001)(81342001)(94946001)(94316002)(87266001)(33646001)(81542001)(47736001)(83072002)(93516002)(74316001)(86362001)(74366001)(50986001)(93136001)(80022001)(69226001)(79102001)(4396001)(59766001)(80976001)(85306002)(49866001)(95416001)(77982001)(24736002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR05MB006;H:BLUPR05MB008.namprd05.prod.outlook.com;CLIP:129.108.37.93;FPR:3ADEF03E.9E339C09.87D2589.84EF8869.201A3;InfoNoRecordsA:0;MX:1;LANG:en; } 6, 29 -- Content-Type: text/plain; charset="Windows-1252" } 6, 29 -- MIME-Version: 1.0 } 6, 29 -- X-OriginatorOrg: miners.utep.edu } 6, 29 -- Content-Transfer-Encoding: 8bit } 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172HCnn004797 } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 49 -- From colijn.1-at-osu.edu Thu Feb 6 20:27:29 2014 8, 49 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1ehsobe004.messaging.microsoft.com [216.32.181.184]) 8, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172RTQS017894 8, 49 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2014 20:27:29 -0600 8, 49 -- Received: from mail80-ch1-R.bigfish.com (10.43.68.251) by 8, 49 -- CH1EHSOBE008.bigfish.com (10.43.70.58) with Microsoft SMTP Server id 8, 49 -- 14.1.225.22; Fri, 7 Feb 2014 02:27:28 +0000 8, 49 -- Received: from mail80-ch1 (localhost [127.0.0.1]) by mail80-ch1-R.bigfish.com 8, 49 -- (Postfix) with ESMTP id 9CCA21404E4 for {microscopy-at-microscopy.com} ; Fri, 7 8, 49 -- Feb 2014 02:27:28 +0000 (UTC) 8, 49 -- X-Forefront-Antispam-Report: CIP:164.107.81.208;KIP:(null);UIP:(null);IPV:NLI;H:cio-krc-pf01;RD:none;EFVD:NLI 8, 49 -- X-SpamScore: 3 8, 49 -- X-BigFish: VPS3(zzbb2dI98dI9371Ic85ehe0eah1432Izz1f42h2148h1d77h208ch1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch1fc6hzd9hz1de098h17326ah8275bh1de097h186068hz2fh2a8h839h946hd25he5bhf0ah1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh162dh1631h1758h1765h18e1h190ch1946h19b4h19c3h19ceh1ad9h1b0ah224fh1d0ch1d2eh1d3fh1dfeh1dffh1f5fh1fe8h1ff5h209eh22d0h2336h2438h2461h2476h2487h24d7h2516h2545h1b1cn1b1bi1155h) 8, 49 -- Received-SPF: pass (mail80-ch1: domain of osu.edu designates 164.107.81.208 as permitted sender) client-ip=164.107.81.208; envelope-from=colijn.1-at-osu.edu; helo=cio-krc-pf01 ;cio-krc-pf01 ; 8, 49 -- Received: from mail80-ch1 (localhost.localdomain [127.0.0.1]) by mail80-ch1 8, 49 -- (MessageSwitch) id 1391740045621553_29835; Fri, 7 Feb 2014 02:27:25 +0000 8, 49 -- (UTC) 8, 49 -- Received: from CH1EHSMHS034.bigfish.com (snatpool1.int.messaging.microsoft.com 8, 49 -- [10.43.68.241]) by mail80-ch1.bigfish.com (Postfix) with ESMTP id 8, 49 -- 9227A2E007D; Fri, 7 Feb 2014 02:27:25 +0000 (UTC) 8, 49 -- Received: from cio-krc-pf01 (164.107.81.208) by CH1EHSMHS034.bigfish.com 8, 49 -- (10.43.70.34) with Microsoft SMTP Server (TLS) id 14.16.227.3; Fri, 7 Feb 8, 49 -- 2014 02:27:25 +0000 8, 49 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) (using TLSv1 8, 49 -- with cipher RC4-MD5 (128/128 bits)) (No client certificate requested) by 8, 49 -- cio-krc-pf01 (Postfix) with ESMTPS id A5FF3A005F; Thu, 6 Feb 2014 21:27:24 8, 49 -- -0500 (EST) 8, 49 -- Received: from [192.168.1.102] (75.118.135.65) by smtp.service.osu.edu 8, 49 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.174.1; Thu, 6 Feb 8, 49 -- 2014 21:27:24 -0500 8, 49 -- Message-ID: {52F4448A.8090904-at-osu.edu} 8, 49 -- Date: Thu, 6 Feb 2014 21:27:22 -0500 8, 49 -- From: Henk Colijn {colijn.1-at-osu.edu} 8, 49 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 8, 49 -- MIME-Version: 1.0 8, 49 -- To: {ahinojos2-at-miners.utep.edu} 8, 49 -- CC: {microscopy-at-microscopy.com} 8, 49 -- Subject: Re: [Microscopy] TEM Acquisition Help 8, 49 -- References: {201402070219.s172JFT5006464-at-ns.microscopy.com} 8, 49 -- In-Reply-To: {201402070219.s172JFT5006464-at-ns.microscopy.com} 8, 49 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 8, 49 -- X-Originating-IP: [75.118.135.65] 8, 49 -- X-CFilter-Loop: Forward 8, 49 -- X-FOPE-CRA-Verdict: 164.107.81.208$miners.utep.edu%0%1%osu.edu%False%False%0$ 8, 49 -- X-OriginatorOrg: osu.edu 8, 49 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 8, 49 -- X-FOPE-CONNECTOR: Id%0$Dn%MINERS.UTEP.EDU$RO%1$TLS%0$FQDN%$TlsDn% 8, 49 -- Content-Transfer-Encoding: 8bit 8, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172RTQS017894 ==============================End of - Headers==============================
A collegue was interested by this instrument last year and made some tests. His goal was to have an easy access to a TEM like instrument, to make fast controls in nanoparticules syntesis (organics with heavy metals), as the acess to our TEMs need some days of delay.
Hearing about the price of that instrument, I proposed him to test too a STEM device to be fitted on our FE-SEM. Comparing prices and performences (principally the versatility), the choice was easy. The STEM device for the SEM gives better images, the possibility to choose the HV up to 30 keV and a price 8x less expensive...
The real adavantage of this table-top TEM I could see is that it can be used easy by non-microcopists, like table-top SEMs, with on the other hand the limitations of the undestanding such people will have of what they do.
So if you soon have a (FE)SEM, it could be usefull to consider if the STEM device for SEM would not be a better solution. But I'm interested on what your tests will bring you as conclusions.
Hope it helps
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Le 07/02/2014 03:28, ahinojos2-at-miners.utep.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hello Microscopists, } Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great. } } } Thanks for your thoughts and answers in advance } Alejandro Hinojos } } } } } ==============================Original Headers============================== } 6, 29 -- From ahinojos2-at-miners.utep.edu Thu Feb 6 20:17:12 2014 } 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) } 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172HCnn004797 } 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Feb 2014 20:17:12 -0600 } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com (10.255.210.155) by } 6, 29 -- BLUPR05MB006.namprd05.prod.outlook.com (10.255.210.142) with Microsoft SMTP } 6, 29 -- Server (TLS) id 15.0.868.8; Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.44]) by } 6, 29 -- BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.156]) with mapi id } 6, 29 -- 15.00.0868.013; Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- From: "Hinojos, Alejandro" {ahinojos2-at-miners.utep.edu} } 6, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 6, 29 -- Subject: TEM Acquisition Help } 6, 29 -- Thread-Topic: TEM Acquisition Help } 6, 29 -- Thread-Index: AQHPI6qIDXb0k8Img0O3WLndhK0Dmg== } 6, 29 -- Date: Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- Message-ID: {1e44fc83cbc14812ac71282723f64e06-at-BLUPR05MB008.namprd05.prod.outlook.com} } 6, 29 -- Accept-Language: en-US } 6, 29 -- Content-Language: en-US } 6, 29 -- X-MS-Has-Attach: } 6, 29 -- X-MS-TNEF-Correlator: } 6, 29 -- x-originating-ip: [129.108.37.93] } 6, 29 -- x-forefront-prvs: 011579F31F } 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(199002)(189002)(74876001)(53806001)(90146001)(76482001)(31966008)(74706001)(76796001)(56816005)(76176001)(75432001)(54316002)(92566001)(81816001)(85852003)(87936001)(63696002)(46102001)(76576001)(76786001)(77096001)(51856001)(54356001)(66066001)(65816001)(74502001)(81686001)(74662001)(47446002)(47976001)(2656002)(83322001)(56776001)(81342001)(94946001)(94316002)(87266001)(33646001)(81542001)(47736001)(83072002)(93516002)(74316001)(86362001)(74366001)(50986001)(93136001)(80022001)(69226001)(79102001)(4396001)(59766001)(80976001)(85306002)(49866001)(95416001)(77982001)(24736002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR05MB006;H:BLUPR05MB008.namprd05.prod.outlook.com;CLIP:129.108.37.93;FPR:3ADEF03E.9E339C09.87D2589.84EF8869.201A3;InfoNoRecordsA:0;MX:1;LANG:en; } 6, 29 -- Content-Type: text/plain; charset="Windows-1252" } 6, 29 -- MIME-Version: 1.0 } 6, 29 -- X-OriginatorOrg: miners.utep.edu } 6, 29 -- Content-Transfer-Encoding: 8bit } 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172HCnn004797 } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 25 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Feb 7 02:01:34 2014 10, 25 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1781YdX013671 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2014 02:01:34 -0600 10, 25 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id 51B6224238B; 10, 25 -- Fri, 7 Feb 2014 09:01:52 +0100 (CET) 10, 25 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr 10, 25 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) 10, 25 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) 10, 25 -- with ESMTP id 458afHncdtNz; Fri, 7 Feb 2014 09:01:51 +0100 (CET) 10, 25 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id 3652524236C; 10, 25 -- Fri, 7 Feb 2014 09:01:51 +0100 (CET) 10, 25 -- Message-ID: {52F492D2.2060601-at-ipcms.u-strasbg.fr} 10, 25 -- Date: Fri, 07 Feb 2014 09:01:22 +0100 10, 25 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 25 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:17.0) Gecko/20130330 Thunderbird/17.0.5 10, 25 -- MIME-Version: 1.0 10, 25 -- To: ahinojos2-at-miners.utep.edu, microscopy-at-microscopy.com 10, 25 -- Subject: Re: [Microscopy] TEM Acquisition Help 10, 25 -- References: {201402070228.s172SkD0020695-at-ns.microscopy.com} 10, 25 -- In-Reply-To: {201402070228.s172SkD0020695-at-ns.microscopy.com} 10, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I have worked with a number of clients over the years, when we have used the SEM in a STEM mode. The advent of FEG systems dramatically changed the capabilities of the SEM to produce good quality STEM images, and the use of these instruments in x-ray analysis has been very fruitful. We have produced a paper that will be in the March "Microscopy Today", this summarises some of the work we have been doing. The use of thin films for x-ray analysis in the SEM, even without a STEM facility, is often a step forward that would have been more difficult to make if a dedicated TEM were used. There are many advantages to using a SEM for thin film x-ray analysis. The spectrum is less likely to be "contaminated" by signals from the instrument itself, and elemental mapping at high resolution is easily accomplished.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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realname - Alejandro Hinojos Email - ahinojos2-at-miners.utep.edu ORGANIZATION - University of Texas at El Paso EDUCATION - Undergraduate College LOCATION - El Paso SUBJECT_OF_QUESTION - TEM Shopping advice QUESTION - Hello Microscopists, Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM's? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great.
Thanks for your thoughts and answers in advance Alejandro Hinojos
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Organization: University of Texas at El Paso, Metalurgical & Materials Engineering Department
Title-Subject: [Filtered] EM Shopping
Message: Hello Microscopists, Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization. If anybody wouldn't mind sharing those experiences with us that would be great.
Thanks for your thoughts and answers in advance Alejandro Hinojos
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While I have a STEM/TEM, from time to time I use SEM/EDS for TEM grids with all advantages Steve mentioned. Just place several TEM grids with thin films on a carbon planchet, and go on! Remember: EDS resolution is determined mostly by thickness of specimen, not by instrument itself. So, EDS spatial resolution in SEM could be measured in nanometers (instead of microns) if thin specimens are used.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Friday, February 07, 2014 4:57 AM To: Dusevich, Vladimir
Hi
I have worked with a number of clients over the years, when we have used the SEM in a STEM mode. The advent of FEG systems dramatically changed the capabilities of the SEM to produce good quality STEM images, and the use of these instruments in x-ray analysis has been very fruitful. We have produced a paper that will be in the March "Microscopy Today", this summarises some of the work we have been doing. The use of thin films for x-ray analysis in the SEM, even without a STEM facility, is often a step forward that would have been more difficult to make if a dedicated TEM were used. There are many advantages to using a SEM for thin film x-ray analysis. The spectrum is less likely to be "contaminated" by signals from the instrument itself, and elemental mapping at high resolution is easily accomplished.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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Email: brian.miller-at-bruker-axs.com Name: brian miller
Organization: Bruker AXS
Title-Subject: [Filtered] EM Shopping
Message: in response to Alejandro's question and several responses I have been reading, I thought it would be good to clarify that the SEM can do nice analytical work in STEM mode and the new Bruker detector called the Flat Quad is a great contribution to this set up as it overcomes the issue of low signal from a TEM sample.
Although the LVEM5 from Delongh might be a very nice imaging system (I don't know but it does look very clever), going to their website and reading the brochure it is obvious that is in no way a system that can be used with any kind of EDS or EELs. So the analytical technique will be based entirely on interpreting the diffraction pattern if it is obtainable.
Than you, Brian Miller Bruker AXS (an OEM for xray detectors on SEM and TEM)
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I was just reading the article in the January 2014 Microscopy Today issue on standardizing color in digital images using the ChromaCal microscope slide and monitor calibration system. Interesting idea but I have a question. I know the authors Barbara Foster and Jerry Sedgewick participate on the listserver so I thought I would post it here for feedback from both them and others who might have used the system.
The website for Datacolor states "The CHROMACAL slide is not suitable for use with oil immersion objectives" but Figures 1 and 4 in the Microscopy Today article were made with oil immersion objectives.
The system looks to cost around $1000 so this won't be an impulse purchase for most of us. Anyone have experience or thoughts on this?
Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax)
==============================Original Headers============================== 7, 28 -- From PhillipsT-at-missouri.edu Mon Feb 10 14:08:10 2014 7, 28 -- Received: from um-nip3-missouri-out.um.umsystem.edu (um-nip3-missouri-out.um.umsystem.edu [198.209.49.163]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1AK8Auc027243 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 14:08:10 -0600 7, 28 -- X-IronPort-Anti-Spam-Filtered: true 7, 28 -- X-IronPort-Anti-Spam-Result: AhUFAC8x+VLPoJ7I/2dsb2JhbABWA4MMgQ+rdpBlgwaBFhZ0gicFgQcEASo6HCYBBBuHfZokqA6HDhcEjkhJgxOBFASJEaE6gy2CKg 7, 28 -- X-IPAS-Result: AhUFAC8x+VLPoJ7I/2dsb2JhbABWA4MMgQ+rdpBlgwaBFhZ0gicFgQcEASo6HCYBBBuHfZokqA6HDhcEjkhJgxOBFASJEaE6gy2CKg 7, 28 -- Received: from um-tcas3.um.umsystem.edu ([207.160.158.200]) 7, 28 -- by um-nip3-exch-relay.um.umsystem.edu with ESMTP; 10 Feb 2014 14:08:09 -0600 7, 28 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) by 7, 28 -- UM-TCAS3.um.umsystem.edu ([207.160.158.200]) with mapi id 14.03.0174.001; 7, 28 -- Mon, 10 Feb 2014 14:08:09 -0600 7, 28 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 7, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 7, 28 -- Subject: ChromaCal 7, 28 -- Thread-Topic: ChromaCal 7, 28 -- Thread-Index: Ac8mluadlg4QWVchQT27kPQM8IwZPgABD2yg 7, 28 -- Date: Mon, 10 Feb 2014 20:08:08 +0000 7, 28 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8EBE7F6-at-UM-MBX-T02.um.umsystem.edu} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- x-originating-ip: [128.206.81.115] 7, 28 -- Content-Type: text/plain; charset="iso-8859-1" 7, 28 -- MIME-Version: 1.0 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1AK8Auc027243 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Electron Microscopist Position Available - The Rockefeller University
Message: The Rockefeller University, a premier biomedical research institution, seeks a Research Support Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state of the art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a free substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Specialist will participate in all of the Electron Microscopy Resource Center's (EMRC) daily operations, including bench work in preparation for transmission and scanning electron microscopy and maintenance of the center. Will be responsible for all parts of sample preparation, including cutting ultrathin sections, maintenance and operation of EM and other equipment, training users, consulting with scientists on design of experiments, processing/analyzing data and interpretation of results, ordering and receiving supplies, managing chemical waste compliance, administrative support for office duties and center billing, and keeping up with the latest methodology via familiarity with the literature. Ph.D. in biology, cell biology, bioengineering or a related field required. Must have a minimum of 5 years of hands-on experience in electron microscopy and have strong communication skills, and the ability to work collaboratively on a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated. Expertise in cryo- 3D is a plus.
Our culture *inspiring, collaborative atmosphere *strong social and environmental consciousness *intellectually curious academic environment *14-acre campus setting in NYC
We offer a competitive salary, comprehensive benefits, and a beautiful work environment. To apply to this job, click the following URL, click on 'staff opportunities’ and enter keyword‘IRC11162’: http://www.rockefeller.edu/hr/career.php The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Kunihiro Uryu, Ph.D., Director of Electron Microscopy Resource Center
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Title-Subject: [Filtered] Parts to give away Zeiss DSM940
Message: Hi everyone, We have a few parts to give away for a SEM Zeiss DSM940(delivery at your charge)(Tungsten filament, stage, detector, etc). Contact me for more information!
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Title-Subject: [Filtered] Parts to give away Zeiss DSM940
Message: Hi everyone, We have a few parts to give away for a SEM Zeiss DSM940(delivery at your charge)(Tungsten filament, stage, detector, etc). Contact me for more information!
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X-from: kuryu-at-mail.rockefeller.edu () This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-mail.rockefeller.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Electron Microscopist Position Available - The Rockefeller University
Message: The Rockefeller University, a premier biomedical research institution, seeks a Research Support Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state of the art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a free substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Specialist will participate in all of the Electron Microscopy Resource Center's (EMRC) daily operations, including bench work in preparation for transmission and scanning electron microscopy and maintenance of the center. Will be responsible for all parts of sample preparation, including cutting ultrathin sections, maintenance and operation of EM and other equipment, training users, consulting with scientists on design of experiments, processing/analyzing data and interpretation of results, ordering and receiving supplies, managing chemical waste compliance, administrative support for office duties and center billing, and keeping up with the latest methodology via familiarity with the literature. Ph.D. in biology, cell biology, bioengineering or a related field required. Must have a minimum of 5 years of hands-on experience in electron microscopy and have strong communication skills, and the ability to work collaboratively on a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated. Expertise in cryo- 3D is a plus.
Our culture *inspiring, collaborative atmosphere *strong social and environmental consciousness *intellectually curious academic environment *14-acre campus setting in NYC
We offer a competitive salary, comprehensive benefits, and a beautiful work environment. To apply to this job, click the following URL, click on 'staff opportunities’ and enter keyword‘IRC11162’: http://www.rockefeller.edu/hr/career.php The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Kunihiro Uryu, Ph.D., Director of Electron Microscopy Resource Center
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==============================Original Headers============================== 18, 29 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb 11 00:52:51 2014 18, 29 -- Received: from znl.com ([206.69.208.20]) 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1B5Qa41022463 18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 23:26:36 -0600 18, 29 -- Received: from localhost (localhost [127.0.0.1]) 18, 29 -- by znl.com (Postfix) with ESMTP id 8CC0C6BA2CA 18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 23:26:36 -0600 (CST) 18, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 29 -- Received: from znl.com ([127.0.0.1]) 18, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 29 -- with ESMTP id Zv-iHGYu3ctM for {microscopy-at-microscopy.com} ; 18, 29 -- Mon, 10 Feb 2014 23:26:23 -0600 (CST) 18, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 29 -- by znl.com (Postfix) with ESMTPA id 2843C6BA2BD 18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 23:26:23 -0600 (CST) 18, 29 -- Message-ID: {52F9B47E.50708-at-microscopy.com} 18, 29 -- Date: Mon, 10 Feb 2014 23:26:22 -0600 18, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 18, 29 -- MIME-Version: 1.0 18, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 29 -- Subject: viaWWW:Electron Microscopist Position Available - The Rockefeller 18, 29 -- University 18, 29 -- References: {201402101542.s1AFgwWa021184-at-ns.microscopy.com} 18, 29 -- In-Reply-To: {201402101542.s1AFgwWa021184-at-ns.microscopy.com} 18, 29 -- X-Forwarded-Message-Id: {201402101542.s1AFgwWa021184-at-ns.microscopy.com} 18, 29 -- Content-Type: text/plain; charset=UTF-8; format=flowed 18, 29 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
True, the ChromaCal slide is not suitable for oil immersion objectives. The slide has no coverslip on the chip, and cleaning may damage the calibration matrix. Figures 1 and 4 were taken using an oil immersion lens for the sample, and a dry objective was used to image the ChromaCal slide. I found I could get equivalent results regardless of the objective used, as long as I kept the white balance the same, and set Koehler illumination.
Having run a light microscopy lab for 15 years, the issue of color inconsistency and inaccuracy led me to attempt making a color calibration slide on my own. I simply could not get the filters small enough for a microscope. Thus, my delight in working with a company that has accomplished it at this scale. To make things even better, I can do morphometry now using consistent color as a means to segment images.
Thanks a million for pointing out the disconnect in the article!
Jerry Sedgewick
On 2/10/2014 2:21 PM, PhillipsT-at-missouri.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was just reading the article in the January 2014 Microscopy Today issue on standardizing color in digital images using the ChromaCal microscope slide and monitor calibration system. Interesting idea but I have a question. I know the authors Barbara Foster and Jerry Sedgewick participate on the listserver so I thought I would post it here for feedback from both them and others who might have used the system. } } The website for Datacolor states "The CHROMACAL slide is not suitable for use with oil immersion objectives" but Figures 1 and 4 in the Microscopy Today article were made with oil immersion objectives. } } The system looks to cost around $1000 so this won't be an impulse purchase for most of us. Anyone have experience or thoughts on this? } } Tom } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } } } } ==============================Original Headers============================== } 7, 28 -- From PhillipsT-at-missouri.edu Mon Feb 10 14:08:10 2014 } 7, 28 -- Received: from um-nip3-missouri-out.um.umsystem.edu (um-nip3-missouri-out.um.umsystem.edu [198.209.49.163]) } 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1AK8Auc027243 } 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 14:08:10 -0600 } 7, 28 -- X-IronPort-Anti-Spam-Filtered: true } 7, 28 -- X-IronPort-Anti-Spam-Result: AhUFAC8x+VLPoJ7I/2dsb2JhbABWA4MMgQ+rdpBlgwaBFhZ0gicFgQcEASo6HCYBBBuHfZokqA6HDhcEjkhJgxOBFASJEaE6gy2CKg } 7, 28 -- X-IPAS-Result: AhUFAC8x+VLPoJ7I/2dsb2JhbABWA4MMgQ+rdpBlgwaBFhZ0gicFgQcEASo6HCYBBBuHfZokqA6HDhcEjkhJgxOBFASJEaE6gy2CKg } 7, 28 -- Received: from um-tcas3.um.umsystem.edu ([207.160.158.200]) } 7, 28 -- by um-nip3-exch-relay.um.umsystem.edu with ESMTP; 10 Feb 2014 14:08:09 -0600 } 7, 28 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) by } 7, 28 -- UM-TCAS3.um.umsystem.edu ([207.160.158.200]) with mapi id 14.03.0174.001; } 7, 28 -- Mon, 10 Feb 2014 14:08:09 -0600 } 7, 28 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 7, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } 7, 28 -- Subject: ChromaCal } 7, 28 -- Thread-Topic: ChromaCal } 7, 28 -- Thread-Index: Ac8mluadlg4QWVchQT27kPQM8IwZPgABD2yg } 7, 28 -- Date: Mon, 10 Feb 2014 20:08:08 +0000 } 7, 28 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8EBE7F6-at-UM-MBX-T02.um.umsystem.edu} } 7, 28 -- Accept-Language: en-US } 7, 28 -- Content-Language: en-US } 7, 28 -- X-MS-Has-Attach: } 7, 28 -- X-MS-TNEF-Correlator: } 7, 28 -- x-originating-ip: [128.206.81.115] } 7, 28 -- Content-Type: text/plain; charset="iso-8859-1" } 7, 28 -- MIME-Version: 1.0 } 7, 28 -- Content-Transfer-Encoding: 8bit } 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1AK8Auc027243 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 37 -- From jerrysedgewick-at-gmail.com Tue Feb 11 08:11:59 2014 9, 37 -- Received: from mail-ig0-f170.google.com (mail-ig0-f170.google.com [209.85.213.170]) 9, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BEBx7H015254 9, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 08:11:59 -0600 9, 37 -- Received: by mail-ig0-f170.google.com with SMTP id m12so8843385iga.1 9, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 06:11:59 -0800 (PST) 9, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 37 -- d=gmail.com; s=20120113; 9, 37 -- h=message-id:date:from:user-agent:mime-version:to:subject:references 9, 37 -- :in-reply-to:content-type:content-transfer-encoding; 9, 37 -- bh=y1XepGT/ku7rbB67You8IUATjg7ZQm51LkKqei248qM=; 9, 37 -- b=K0g4FiTvCk09CcSQDySnYQXoz3o9FAxcY/xBxqA1GQv1K+Y3M4We53g48IZd2c4dgU 9, 37 -- U9Op11BkNUWfw0t48nTlACHB7OZjF6pkIqg8oGiwg37camOeayGTJiVAcc2etgW7aHMx 9, 37 -- tTgW7GfSw67fx25zKV8TTgRZ/AySIrOdEU6Z4IRbvYCRIkkAMvg+6JU370uekINBSRtT 9, 37 -- poO5Et2IHIqF+QQlx4HGhmXiG6mle4+/pOUK2ohS0kirVOtZttvS0xsAwSvTJnqD5Cpj 9, 37 -- B7Ay8U1+FRkFWKKO/xKtw263dXxEFTlZw/nLcGiZrcyOZcFMzlz7gKxHpdZgXFm1LeM2 9, 37 -- CqIg== 9, 37 -- X-Received: by 10.50.23.112 with SMTP id l16mr19766172igf.28.1392127919342; 9, 37 -- Tue, 11 Feb 2014 06:11:59 -0800 (PST) 9, 37 -- Received: from [127.0.0.1] (184-97-147-16.mpls.qwest.net. [184.97.147.16]) 9, 37 -- by mx.google.com with ESMTPSA id vn3sm15074124igb.0.2014.02.11.06.11.58 9, 37 -- for {Microscopy-at-microscopy.com} 9, 37 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 9, 37 -- Tue, 11 Feb 2014 06:11:58 -0800 (PST) 9, 37 -- Message-ID: {52FA2FAB.3030401-at-gmail.com} 9, 37 -- Date: Tue, 11 Feb 2014 08:11:55 -0600 9, 37 -- From: jerry sedgewick {jerrysedgewick-at-gmail.com} 9, 37 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 9, 37 -- MIME-Version: 1.0 9, 37 -- To: Microscopy-at-microscopy.com 9, 37 -- Subject: Re: [Microscopy] ChromaCal 9, 37 -- References: {201402102021.s1AKLFEK006884-at-ns.microscopy.com} 9, 37 -- In-Reply-To: {201402102021.s1AKLFEK006884-at-ns.microscopy.com} 9, 37 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 37 -- Content-Transfer-Encoding: 7bit 9, 37 -- X-Antivirus: avast! (VPS 140211-0, 02/11/2014), Outbound message 9, 37 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both draichle-at-tampabay.rr.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: draichle-at-tampabay.rr.com Name: Dr Erik Raichle
Organization: Raichle Diagnostic
Title-Subject: [Filtered] Specimen Size and The Limits of Lens Optics?
Message: I have 2 questions:
IÂ’ve asked this question of many people, therefore,
About what size would a specimen be when viewed through a microscope at 1000x? 70um?
Will we ever be able to view a specimen at 1000x with a field of view of 1cm and a depth of view of 1cm?
Or, would this exceed the limits of lens of optics?
Also, do we have any other technology beside lens optics that could image a 100um, or less, specimen, such as, sonor or radar or electron beam that could be handheld?
We have identified and lost an extraordinary Mite specimen a person's skin that was less than 100um. It was extraordinary because it looked like a spider, mites that small are wormlike. We believe it is an unclassified pathogen and very much want to capture these specimens on peopleÂ’s skin if we can.
Or perhaps, there is a procedure that will immobilize them and isolate them under a microscope?
Anything you can think of or person who you can refer us to contact, would be most appreciated. Thank you, Dr Erik Raichle
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==============================Original Headers============================== 21, 29 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb 11 10:22:06 2014 21, 29 -- Received: from znl.com ([206.69.208.20]) 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BGM66d004271 21, 29 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 10:22:06 -0600 21, 29 -- Received: from localhost (localhost [127.0.0.1]) 21, 29 -- by znl.com (Postfix) with ESMTP id 789536BA859 21, 29 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 10:22:06 -0600 (CST) 21, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 21, 29 -- Received: from znl.com ([127.0.0.1]) 21, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 21, 29 -- with ESMTP id JLYg9GGlR9Yf for {microscopy-at-microscopy.com} ; 21, 29 -- Tue, 11 Feb 2014 10:22:02 -0600 (CST) 21, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 21, 29 -- by znl.com (Postfix) with ESMTPA id 73F526BA84C 21, 29 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 10:22:02 -0600 (CST) 21, 29 -- Message-ID: {52FA4E2A.2010907-at-microscopy.com} 21, 29 -- Date: Tue, 11 Feb 2014 10:22:02 -0600 21, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 21, 29 -- MIME-Version: 1.0 21, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 29 -- Subject: Fwd: [Filtered] MicroscopyListserverviaWWW:Specimen Size and The 21, 29 -- Limits of Lens Optics? 21, 29 -- References: {201402111551.s1BFpgF2003772-at-ns.microscopy.com} 21, 29 -- In-Reply-To: {201402111551.s1BFpgF2003772-at-ns.microscopy.com} 21, 29 -- X-Forwarded-Message-Id: {201402111551.s1BFpgF2003772-at-ns.microscopy.com} 21, 29 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 29 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I have a customer who needs to measure the coating on 300 micron diameter sodium sulfate core samples coated with ethyl cellulose. The core surface is rough. Customer would like to embed in a suitable resin that will not dissolve the ethyl cellulose coating or cause the coating to shrink/swell. Can someone recommend an embedding media so we can pot and crossection on a microtome for SEM ?
Thank you
Fred Hayes Manager Advanced Materials Characterization and Testing Lab (AMCaT) Dept of Chemical Engineering and Material Science 3001 Ghausi Hall Univ of CA Davis Davis, CA 95616 530-752-0284 chms.engineering.ucdavis.edu/index.html chms.engineering.ucdavis.edu/research/amcat/index.html
==============================Original Headers============================== 6, 22 -- From fahayes-at-ucdavis.edu Tue Feb 11 13:04:53 2014 6, 22 -- Received: from smtp3.ucdavis.edu (smtp3.ucdavis.edu [128.120.32.129]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BJ4r3e025872 6, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 13:04:53 -0600 6, 22 -- Received: from chmsk011201 ([169.237.64.243]) 6, 22 -- by smtp3.ucdavis.edu (8.14.4/8.14.5/it-rhel5-mimedefang-smtp-1.6) with ESMTP id s1BJ4nLA056558 6, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 11:04:50 -0800 6, 22 -- From: "Fred Hayes" {fahayes-at-ucdavis.edu} 6, 22 -- To: {Microscopy-at-microscopy.com} 6, 22 -- Subject: need a recommendation for embedding media 6, 22 -- Date: Tue, 11 Feb 2014 11:04:48 -0800 6, 22 -- Message-ID: {006d01cf275c$23d21a00$6b764e00$-at-ucdavis.edu} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain; 6, 22 -- charset="us-ascii" 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-Mailer: Microsoft Outlook 14.0 6, 22 -- Thread-Index: Ac8nW4CkWM/KhE91R7y6XwCA9Qbaeg== 6, 22 -- Content-Language: en-us 6, 22 -- X-Virus-Scanned: clamav-milter 0.98.1 at smtp3 6, 22 -- X-Virus-Status: Clean 6, 22 -- X-Scanned-By: MIMEDefang 2.74 ==============================End of - Headers==============================
Dr. Raichle; To view a specimen with a 1 cm field of view at 1,000X you would need to have a computer monitor 10 meters across. So it IS possible, just difficult without a Jumbo Tron. Then you would have to stand so far back to see it, that it would get small due to being far away, so what would be the point?
John Mardinly, ASU
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both draichle-at-tampabay.rr.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: draichle-at-tampabay.rr.com Name: Dr Erik Raichle
Organization: Raichle Diagnostic
Title-Subject: [Filtered] Specimen Size and The Limits of Lens Optics?
Message: I have 2 questions:
IÂ've asked this question of many people, therefore,
About what size would a specimen be when viewed through a microscope at 1000x? 70um?
Will we ever be able to view a specimen at 1000x with a field of view of 1cm and a depth of view of 1cm?
Or, would this exceed the limits of lens of optics?
Also, do we have any other technology beside lens optics that could image a 100um, or less, specimen, such as, sonor or radar or electron beam that could be handheld?
We have identified and lost an extraordinary Mite specimen a person's skin that was less than 100um. It was extraordinary because it looked like a spider, mites that small are wormlike. We believe it is an unclassified pathogen and very much want to capture these specimens on peopleÂ's skin if we can.
Or perhaps, there is a procedure that will immobilize them and isolate them under a microscope?
Anything you can think of or person who you can refer us to contact, would be most appreciated. Thank you, Dr Erik Raichle
Login Host: 173.168.136.150 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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==============================Original Headers============================== 27, 43 -- From John.Mardinly-at-asu.edu Tue Feb 11 17:36:54 2014 27, 43 -- Received: from bcnet7.asu.edu (bcnet7.asu.edu [129.219.117.222]) 27, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BNas7o016112 27, 43 -- for {Microscopy-at-Microscopy.com} ; Tue, 11 Feb 2014 17:36:54 -0600 27, 43 -- X-ASG-Debug-ID: 1392161813-04dc7a6a3934990001-FOsErg 27, 43 -- Received: from exhubt01.asurite.ad.asu.edu (exhubt01.asurite.ad.asu.edu [129.219.103.40]) by bcnet7.asu.edu with ESMTP id sK0cUJLL3XCtMgoL (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Tue, 11 Feb 2014 16:36:53 -0700 (MST) 27, 43 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 27, 43 -- X-Barracuda-Apparent-Source-IP: 129.219.103.40 27, 43 -- X-ASG-Whitelist: Client 27, 43 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 27, 43 -- exhubt01.asurite.ad.asu.edu ([129.219.103.40]) with mapi id 14.03.0174.001; 27, 43 -- Tue, 11 Feb 2014 16:36:52 -0700 27, 43 -- From: John Mardinly {John.Mardinly-at-asu.edu} 27, 43 -- To: "microscopylistserver-noreply-at-microscopy.com" 27, 43 -- {microscopylistserver-noreply-at-Microscopy.com} , 27, 43 -- "Microscopy-at-Microscopy.com" 27, 43 -- {Microscopy-at-Microscopy.com} 27, 43 -- Subject: RE: [Microscopy] Fwd: [Filtered] 27, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 27, 43 -- Thread-Topic: [Microscopy] Fwd: [Filtered] 27, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 27, 43 -- X-ASG-Orig-Subj: RE: [Microscopy] Fwd: [Filtered] 27, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 27, 43 -- Thread-Index: AQHPJ0cRbqiyodXEfk+A8PkB3ijSEpqwtAhg 27, 43 -- Date: Tue, 11 Feb 2014 23:36:51 +0000 27, 43 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400FFC0AB2-at-exmbt05.asurite.ad.asu.edu} 27, 43 -- References: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 27, 43 -- In-Reply-To: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 27, 43 -- Accept-Language: en-US 27, 43 -- Content-Language: en-US 27, 43 -- X-MS-Has-Attach: 27, 43 -- X-MS-TNEF-Correlator: 27, 43 -- x-originating-ip: [129.219.103.4] 27, 43 -- Content-Type: text/plain; charset="iso-8859-1" 27, 43 -- MIME-Version: 1.0 27, 43 -- X-Barracuda-Connect: exhubt01.asurite.ad.asu.edu[129.219.103.40] 27, 43 -- X-Barracuda-Start-Time: 1392161813 27, 43 -- X-Barracuda-Encrypted: AES128-SHA 27, 43 -- X-Barracuda-URL: http://129.219.117.222:8000/cgi-mod/mark.cgi 27, 43 -- X-Virus-Scanned: by bsmtpd at asu.edu 27, 43 -- X-Barracuda-BRTS-Status: 1 27, 43 -- Content-Transfer-Encoding: 8bit 27, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1BNas7o016112 ==============================End of - Headers==============================
Dr. Raichle; To view a specimen with a 1 cm field of view at 1,000X you would need to have a computer monitor 10 meters across. So it IS possible, just difficult without a Jumbo Tron. Then you would have to stand so far back to see it, that it would get small due to being far away, so what would be the point?
John Mardinly, ASU
==============================Original Headers============================== 4, 43 -- From John.Mardinly-at-asu.edu Tue Feb 11 17:39:08 2014 4, 43 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 4, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BNd8Um018171 4, 43 -- for {Microscopy-at-Microscopy.com} ; Tue, 11 Feb 2014 17:39:08 -0600 4, 43 -- X-ASG-Debug-ID: 1392161947-06a86756a634050001-FOsErg 4, 43 -- Received: from exhubt01.asurite.ad.asu.edu (exhubt01.asurite.ad.asu.edu [129.219.103.40]) by bcnet8.asu.edu with ESMTP id Lbp4618YAwoIEanC (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Tue, 11 Feb 2014 16:39:07 -0700 (MST) 4, 43 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 43 -- X-Barracuda-Apparent-Source-IP: 129.219.103.40 4, 43 -- X-ASG-Whitelist: Client 4, 43 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 4, 43 -- exhubt01.asurite.ad.asu.edu ([129.219.103.40]) with mapi id 14.03.0174.001; 4, 43 -- Tue, 11 Feb 2014 16:39:07 -0700 4, 43 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 43 -- To: "microscopylistserver-noreply-at-microscopy.com" 4, 43 -- {microscopylistserver-noreply-at-Microscopy.com} , 4, 43 -- "Microscopy-at-Microscopy.com" 4, 43 -- {Microscopy-at-Microscopy.com} 4, 43 -- Subject: RE: [Microscopy] Fwd: [Filtered] 4, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 43 -- Thread-Topic: [Microscopy] Fwd: [Filtered] 4, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 43 -- X-ASG-Orig-Subj: RE: [Microscopy] Fwd: [Filtered] 4, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 43 -- Thread-Index: AQHPJ0cRbqiyodXEfk+A8PkB3ijSEpqwtgRg 4, 43 -- Date: Tue, 11 Feb 2014 23:39:04 +0000 4, 43 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400FFC1ADF-at-exmbt05.asurite.ad.asu.edu} 4, 43 -- References: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 4, 43 -- In-Reply-To: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 4, 43 -- Accept-Language: en-US 4, 43 -- Content-Language: en-US 4, 43 -- X-MS-Has-Attach: 4, 43 -- X-MS-TNEF-Correlator: 4, 43 -- x-originating-ip: [129.219.103.4] 4, 43 -- Content-Type: text/plain; charset="us-ascii" 4, 43 -- MIME-Version: 1.0 4, 43 -- X-Barracuda-Connect: exhubt01.asurite.ad.asu.edu[129.219.103.40] 4, 43 -- X-Barracuda-Start-Time: 1392161947 4, 43 -- X-Barracuda-Encrypted: AES128-SHA 4, 43 -- X-Barracuda-URL: http://129.219.117.210:8000/cgi-mod/mark.cgi 4, 43 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 43 -- X-Barracuda-BRTS-Status: 1 4, 43 -- Content-Transfer-Encoding: 8bit 4, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1BNd8Um018171 ==============================End of - Headers==============================
Dr. Raichle; To view a specimen with a 1 cm field of view at 1,000X you would need to have a computer monitor 10 meters across. So it IS possible, just difficult without a Jumbo Tron. Then you would have to stand so far back to see it, that it would get small due to being far away, so what would be the point?
John Mardinly, ASU
==============================Original Headers============================== 4, 40 -- From John.Mardinly-at-asu.edu Tue Feb 11 17:43:30 2014 4, 40 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BNhUEX029653 4, 40 -- for {Microscopy-at-Microscopy.com} ; Tue, 11 Feb 2014 17:43:30 -0600 4, 40 -- X-ASG-Debug-ID: 1392162208-06a86756a136970001-FOsErg 4, 40 -- Received: from exhubt04.asurite.ad.asu.edu (exhubt04.asurite.ad.asu.edu [129.219.103.43]) by bcnet8.asu.edu with ESMTP id x2NgXb40p3QutSkv (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Tue, 11 Feb 2014 16:43:28 -0700 (MST) 4, 40 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 40 -- X-Barracuda-Apparent-Source-IP: 129.219.103.43 4, 40 -- X-ASG-Whitelist: Client 4, 40 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 4, 40 -- exhubt04.asurite.ad.asu.edu ([129.219.103.43]) with mapi id 14.03.0174.001; 4, 40 -- Tue, 11 Feb 2014 16:43:28 -0700 4, 40 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 40 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 4, 40 -- Subject: RE: [Microscopy] Fwd: [Filtered] 4, 40 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 40 -- Thread-Topic: [Microscopy] Fwd: [Filtered] 4, 40 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 40 -- X-ASG-Orig-Subj: RE: [Microscopy] Fwd: [Filtered] 4, 40 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 40 -- Thread-Index: AQHPJ0cRbqiyodXEfk+A8PkB3ijSEpqwt1MQ 4, 40 -- Date: Tue, 11 Feb 2014 23:43:28 +0000 4, 40 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400FFC1B3D-at-exmbt05.asurite.ad.asu.edu} 4, 40 -- References: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 4, 40 -- In-Reply-To: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 4, 40 -- Accept-Language: en-US 4, 40 -- Content-Language: en-US 4, 40 -- X-MS-Has-Attach: 4, 40 -- X-MS-TNEF-Correlator: 4, 40 -- x-originating-ip: [129.219.103.4] 4, 40 -- Content-Type: text/plain; charset="us-ascii" 4, 40 -- MIME-Version: 1.0 4, 40 -- X-Barracuda-Connect: exhubt04.asurite.ad.asu.edu[129.219.103.43] 4, 40 -- X-Barracuda-Start-Time: 1392162208 4, 40 -- X-Barracuda-Encrypted: AES128-SHA 4, 40 -- X-Barracuda-URL: http://129.219.117.210:8000/cgi-mod/mark.cgi 4, 40 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 40 -- X-Barracuda-BRTS-Status: 1 4, 40 -- Content-Transfer-Encoding: 8bit 4, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1BNhUEX029653 ==============================End of - Headers==============================
I hope all is well. If you are interested in advancing your EELS and EFTEM (analytical TEM) knowledge, Gatan is offering a 4 day professional training school on April 8-11, 2014 at its R&D Headquarters in Pleasanton, CA, USA. Here are all the details and online registration: http://www.gatan.com/resources/training/EELSSchoolApr2014.php
EELS & EFTEM Analysis Training School Gatan, Inc. Pleasanton, California, USA April 8-11, 2014
Want to refresh or advance your EELS and EFTEM knowledge? Join us for an intensive 4-day training school that incorporates lectures, computer laboratories, and microscope practicals to provide participants with comprehensive, hands-on training on key EELS and EFTEM topics and technology.
Online registration is now open. Click here {http://www.gatan.com/resources/training/EELSSchoolApr2014.php} . Space is limited.
Overview: Transmission electron microscopy (TEM) reveals details of natural and man-made structures at the micrometer, nanometer, and even sub-nanometer scale. Energy-filtered TEM (EFTEM) and electron energy-loss spectroscopy (EELS) are the ideal analytical partners to the high spatial resolution provided by TEM in both the conventional and scanned (STEM) imaging modes.
This course reviews the basic theory and practice of EELS imaging and analysis in the TEM, but its main emphasis is on practical techniques, optimum deployment of Gatan hardware and software systems, and advanced EELS and EFTEM applications. Some prior experience with EELS, EFTEM, and Gatan systems is recommended, as is a good familiarity with TEM/STEM instrumentation and techniques. By the end of the course, participants can expect to know how best to optimize the performance of their Gatan EELS hardware as well as their EELS and EFTEM experimental setups in order to capture and extract the maximum amount of information from their TEM samples.
Topics
* Fundamentals of EELS and energy-filtered imaging in TEM
* Principles of operation of Gatan EFTEM and EELS systems
* Optimization of EFTEM and EELS data acquisition
* Quantification of elemental composition
* Other information provided by EFTEM/EELS and how best to extract it
* Use of EELS signals to form maps of elemental and chemical composition
* EFTEM and STEM EELS spectrum imaging techniques
* Identification of material phases via EELS fine structure mapping
* Applications to biological and physical science specimens
-- Best regards,
John Marketing Communications Manager
Gatan, Inc.
Email: info-at-gatan.com Website: www.gatan.com
==============================Original Headers============================== 15, 25 -- From JHyun-at-gatan.com Tue Feb 11 17:57:23 2014 15, 25 -- Received: from email.gatan.com (autodiscover.gatan.com [50.58.128.3]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BNvMqA007937 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 17:57:22 -0600 15, 25 -- Received: from MSE.gatan.com ([fe80::4968:b1cf:a686:47eb]) by email.gatan.com 15, 25 -- ([fe80::5125:aff1:b4a6:ad6e%10]) with mapi id 14.03.0174.001; Tue, 11 Feb 15, 25 -- 2014 15:57:21 -0800 15, 25 -- From: John Hyun {JHyun-at-gatan.com} 15, 25 -- To: Microscopy Server {microscopy-at-microscopy.com} 15, 25 -- Subject: EELS and EFTEM Training School on April 8-11, 2014 15, 25 -- Thread-Topic: EELS and EFTEM Training School on April 8-11, 2014 15, 25 -- Thread-Index: AQHPJ4UAzj47fqt6vEO5uWMdfiSf5w== 15, 25 -- Date: Tue, 11 Feb 2014 23:57:20 +0000 15, 25 -- Message-ID: {CF1FF826.2F8A0%jhyun-at-gatan.com} 15, 25 -- In-Reply-To: {CF0FDB05.2F1FA%jhyun-at-gatan.com} 15, 25 -- Accept-Language: en-US 15, 25 -- Content-Language: en-US 15, 25 -- X-MS-Has-Attach: 15, 25 -- X-MS-TNEF-Correlator: 15, 25 -- x-originating-ip: [10.13.7.151] 15, 25 -- Content-Type: text/plain; charset="us-ascii" 15, 25 -- Content-ID: {46FFE2A191A9A14E9609E8C018C5F06F-at-gatan.com} 15, 25 -- MIME-Version: 1.0 15, 25 -- Content-Transfer-Encoding: 8bit 15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1BNvMqA007937 ==============================End of - Headers==============================
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Message: Upon first turning our newly renovated Philips CM10 TEM, we cannot get any of the functions to work. The standby light on the power supply unit is illuminated as well as the ON button by the display monitor on the electronics rack. When the ON button by the monitor is pressed, the column makes a sort of electronic noise. Other than that there seems to be no power getting to the monitor or any controls. Any idea on what our problem may be? Are there any controls on the power supply that need to be turned on other than the MAINS dial?
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb 11 18:46:39 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C0kdv9027338 12, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:46:39 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id EA1516BACDF 12, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:46:38 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id 7a8Ynqwxeyzi for {microscopy-at-microscopy.com} ; 12, 28 -- Tue, 11 Feb 2014 18:46:37 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id EF9026BACD2 12, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:46:37 -0600 (CST) 12, 28 -- Message-ID: {52FAC46D.3080209-at-microscopy.com} 12, 28 -- Date: Tue, 11 Feb 2014 18:46:37 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:CM10 troubleshooting 12, 28 -- References: {201402111919.s1BJJkeC012484-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201402111919.s1BJJkeC012484-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201402111919.s1BJJkeC012484-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howar Hughes Research Institute
Title-Subject: [Filtered] Re: need a recommendation for embedding media
Message: Back when I was doing materials science samples I always used Epo-Fix. Check the Electron Microscopy Sciences website under Materials Science and Meteorology } Embedding, and there are a lot of new embedding media that may help you.
Best to you,
Lita
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb 11 18:47:28 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C0lSt4028291 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:47:28 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 7E1936BACF0 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:47:28 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id dj4QXx5F0KO2 for {microscopy-at-microscopy.com} ; 14, 28 -- Tue, 11 Feb 2014 18:47:27 -0600 (CST) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id BAE516BACE3 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:47:27 -0600 (CST) 14, 28 -- Message-ID: {52FAC49F.9040709-at-microscopy.com} 14, 28 -- Date: Tue, 11 Feb 2014 18:47:27 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:need a recommendation for embedding media 14, 28 -- References: {201402111945.s1BJjne6012865-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201402111945.s1BJjne6012865-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201402111945.s1BJjne6012865-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Josh, did you check air pressure (4 to 5 bar) and cooling water flow rate? There are saftey switches which should be activated.
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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==============================Original Headers============================== 7, 22 -- From stefan.diller-at-t-online.de Wed Feb 12 00:44:20 2014 7, 22 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C6iJqg004234 7, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 00:44:19 -0600 7, 22 -- Received: from fwd09.aul.t-online.de (fwd09.aul.t-online.de ) 7, 22 -- by mailout08.t-online.de with smtp 7, 22 -- id 1WDTYP-0001cE-CF; Wed, 12 Feb 2014 07:44:17 +0100 7, 22 -- Received: from mac-pro.speedport.ip (bKTePeZcwh1HeefOCFYr5Td4040Y-9vbkM4a3P0iPK8i9su1SRc5Ee1tF0jfr1+Q-z-at-[84.174.98.43]) by fwd09.t-online.de 7, 22 -- with esmtp id 1WDTYH-1Xt7NQ0; Wed, 12 Feb 2014 07:44:09 +0100 7, 22 -- Message-ID: {52FB183A.60505-at-t-online.de} 7, 22 -- Date: Wed, 12 Feb 2014 07:44:10 +0100 7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 7, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 7, 22 -- MIME-Version: 1.0 7, 22 -- To: microscopy-at-microscopy.com, jcsmtf-at-mail.missouri.edu 7, 22 -- Subject: Re: [Microscopy] viaWWW:CM10 troubleshooting 7, 22 -- References: {201402120052.s1C0q3Kb005834-at-ns.microscopy.com} 7, 22 -- In-Reply-To: {201402120052.s1C0q3Kb005834-at-ns.microscopy.com} 7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- X-ID: bKTePeZcwh1HeefOCFYr5Td4040Y-9vbkM4a3P0iPK8i9su1SRc5Ee1tF0jfr1+Q-z 7, 22 -- X-TOI-MSGID: 87cc288e-c675-489c-af30-4eaafad98669 ==============================End of - Headers==============================
We are currently searching for two SEMs in particular one equipped with a focussed ion beam column and mass spectrometry capabilities. Are any of you aware of manufacturers and does anyone have experience with such instruments?
The formal request for information is available in this link - https://www.ungm.org/Public/Notice/25524
Thanks a lot, Ernesto
Mr Ernesto CHINEA-CANO | Laboratory Assistant | Office of Safeguards Analytical Services | Environmental Sample Laboratory | Department of Safeguards | International Atomic Energy Agency | IAEA Laboratories, Reaktorstrasse 1, A-2444 Seibersdorf, Austria| Email: E.Chinea-Cano-at-iaea.org | T: (+43-1) 2600-28583 | F: (+43-1) 2600-28577| Follow us on www.iaea.org
This email message is intended only for the use of the named recipient. Information contained in this email message and its attachments may be privileged, confidential and protected from disclosure. If you are not the intended recipient, please do not read, copy, use or disclose this communication to others. Also please notify the sender by replying to this message and then delete it from your system.
==============================Original Headers============================== 8, 42 -- From E.Chinea-Cano-at-iaea.org Wed Feb 12 02:18:58 2014 8, 42 -- Received: from vs-m201.iaea.org (vs-m201.iaea.org [161.5.6.178]) 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C8IvRj024502 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 02:18:58 -0600 8, 42 -- Received: from vs-m2.iaea.org (vs-mail2.iaea.org [172.24.1.27]) 8, 42 -- by vs-m201.iaea.org (8.14.3/8.14.3/SuSE Linux 0.8) with ESMTP id s1C8ItBM032619 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 09:18:57 +0100 8, 42 -- Received: from E2.iaea.org (e2.iaea.org [172.24.0.42]) 8, 42 -- by vs-m2.iaea.org (8.14.3/8.14.3/SuSE Linux 0.8) with ESMTP id s1C8ItYs005262 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 09:18:55 +0100 8, 42 -- X-Envelope-Sender: E.Chinea-Cano-at-iaea.org 8, 42 -- Received: from SEM001PD.sg.iaea.org (161.5.105.92) by E2.iaea.org 8, 42 -- (172.24.0.42) with Microsoft SMTP Server (TLS) id 14.1.438.0; Wed, 12 Feb 8, 42 -- 2014 09:18:54 +0100 8, 42 -- Received: from SEM002PD.sg.iaea.org ([161.5.105.93]) by sem001pd.sg.iaea.org 8, 42 -- ([161.5.105.92]) with mapi id 14.01.0438.000; Wed, 12 Feb 2014 09:18:54 +0100 8, 42 -- From: {E.Chinea-Cano-at-iaea.org} 8, 42 -- To: {Microscopy-at-microscopy.com} 8, 42 -- CC: {H.Gronnerod-at-iaea.org} , {N.DZIGAL-at-iaea.org} , {K.Vogt-at-iaea.org} 8, 42 -- Subject: (SEM) The IAEA is seeking manufacturers/authorized agents 8, 42 -- Thread-Topic: (SEM) The IAEA is seeking manufacturers/authorized agents 8, 42 -- Thread-Index: Ac8nyFf48LYAowLSSxKP2KOHn2UcJQ== 8, 42 -- Date: Wed, 12 Feb 2014 08:18:53 +0000 8, 42 -- Message-ID: {2D1B242827F0914FB9F4B77825D49A82C6B829B3-at-sem002pd.sg.iaea.org} 8, 42 -- Accept-Language: en-GB, en-US 8, 42 -- Content-Language: en-US 8, 42 -- X-MS-Has-Attach: 8, 42 -- X-MS-TNEF-Correlator: 8, 42 -- x-originating-ip: [161.5.105.94] 8, 42 -- Content-Type: text/plain; charset="us-ascii" 8, 42 -- MIME-Version: 1.0 8, 42 -- X-TM-AS-Product-Ver: SMEX-10.0.0.1412-7.000.1014-20496.005 8, 42 -- X-TM-AS-Result: No--8.715000-0.000000-31 8, 42 -- X-TM-AS-User-Approved-Sender: Yes 8, 42 -- X-TM-AS-User-Blocked-Sender: No 8, 42 -- X-Anti-Virus: Kaspersky Anti-Virus for Linux Mail Server 5.6.48/RELEASE, 8, 42 -- bases: 20140211 #7507362, check: 20140212 clean 8, 42 -- X-Anti-Virus: Kaspersky Anti-Virus for Linux Mail Server 5.6.48/RELEASE, 8, 42 -- bases: 20140211 #7507362, check: 20140212 clean 8, 42 -- X-Scanned-By: MIMEDefang 2.73 8, 42 -- Content-Transfer-Encoding: 8bit 8, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1C8IvRj024502 ==============================End of - Headers==============================
Following my e-mail in answer to Alejandro Hinojos's question, I was contacted by the DeLonghi representative. So I want just to add some precisions to my former comments.
First, I want to point out that I didn't gave any feedback about the quality of the results that come out of this instrument. To be right and fair, I must say that the tests which were made for us gave pictures in the same quality range than with the STEM device for SEM. As we didn't perform the test ourself, I cannot say anything about the ease of use of the instrument and so on.
Secondly our final choice has ben dictated by to main raisons : -first we have a FE-SEM -second we didn't have the monney for the DeLonghi intrument and no hope to get it in a near future. But we had enough for the STEM device, to be fitted to the existing FE-SEM. The global result is less expend and more flexibility, but less troughput possibility and no self-service use.
It is evident that all situations are not the same. A lab which has no SEM or TEM can have benefit to spend 150 kE in a table-tob TEM/SEM for a fast and easy to use instrument. Or a big EM service may have an interest too, as a tool usefull to sort TEM grids before going to the high end TEM.
I hope that these comments will clarify the contexte of my former mail. My goal was only to point out an other possibility as Alejandro Hinojos said they have soon SEM and TEM in their lab.
Best Regards
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Le 07/02/2014 09:12, jacques.faerber-at-ipcms.u-strasbg.fr a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } } A collegue was interested by this instrument last year and made some } tests. His goal was to have an easy access to a TEM like instrument, to } make fast controls in nanoparticules syntesis (organics with heavy } metals), as the acess to our TEMs need some days of delay. } } Hearing about the price of that instrument, I proposed him to test too a } STEM device to be fitted on our FE-SEM. } Comparing prices and performences (principally the versatility), the } choice was easy. The STEM device for the SEM gives better images, the } possibility to choose the HV up to 30 keV and a price 8x less expensive... } } The real adavantage of this table-top TEM I could see is that it can be } used easy by non-microcopists, like table-top SEMs, with on the other } hand the limitations of the undestanding such people will have of what } they do. } } So if you soon have a (FE)SEM, it could be usefull to consider if the } STEM device for SEM would not be a better solution. But I'm interested } on what your tests will bring you as conclusions. } } Hope it helps } } J. Faerber } IPCMS-DSI } Institut de Physique et Chimie des Matériaux de Strasbourg } Département Surfaces et Interfaces } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail : Jacques.Faerber-at-ipcms.unistra.fr } } Le 07/02/2014 03:28, ahinojos2-at-miners.utep.edu a écrit : } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } Hello Microscopists, } } Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great. } } } } } } Thanks for your thoughts and answers in advance } } Alejandro Hinojos } } } } } } } } } } ==============================Original Headers============================== } } 6, 29 -- From ahinojos2-at-miners.utep.edu Thu Feb 6 20:17:12 2014 } } 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) } } 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172HCnn004797 } } 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Feb 2014 20:17:12 -0600 } } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com (10.255.210.155) by } } 6, 29 -- BLUPR05MB006.namprd05.prod.outlook.com (10.255.210.142) with Microsoft SMTP } } 6, 29 -- Server (TLS) id 15.0.868.8; Fri, 7 Feb 2014 02:17:10 +0000 } } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.44]) by } } 6, 29 -- BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.156]) with mapi id } } 6, 29 -- 15.00.0868.013; Fri, 7 Feb 2014 02:17:10 +0000 } } 6, 29 -- From: "Hinojos, Alejandro" {ahinojos2-at-miners.utep.edu} } } 6, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } } 6, 29 -- Subject: TEM Acquisition Help } } 6, 29 -- Thread-Topic: TEM Acquisition Help } } 6, 29 -- Thread-Index: AQHPI6qIDXb0k8Img0O3WLndhK0Dmg== } } 6, 29 -- Date: Fri, 7 Feb 2014 02:17:10 +0000 } } 6, 29 -- Message-ID: {1e44fc83cbc14812ac71282723f64e06-at-BLUPR05MB008.namprd05.prod.outlook.com} } } 6, 29 -- Accept-Language: en-US } } 6, 29 -- Content-Language: en-US } } 6, 29 -- X-MS-Has-Attach: } } 6, 29 -- X-MS-TNEF-Correlator: } } 6, 29 -- x-originating-ip: [129.108.37.93] } } 6, 29 -- x-forefront-prvs: 011579F31F } } 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(199002)(189002)(74876001)(53806001)(90146001)(76482001)(31966008)(74706001)(76796001)(56816005)(76176001)(75432001)(54316002)(92566001)(81816001)(85852003)(87936001)(63696002)(46102001)(76576001)(76786001)(77096001)(51856001)(54356001)(66066001)(65816001)(74502001)(81686001)(74662001)(47446002)(47976001)(2656002)(83322001)(56776001)(81342001)(94946001)(94316002)(87266001)(33646001)(81542001)(47736001)(83072002)(93516002)(74316001)(86362001)(74366001)(50986001)(93136001)(80022001)(69226001)(79102001)(4396001)(59766001)(80976001)(85306002)(49866001)(95416001)(77982001)(24736002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR05MB006;H:BLUPR05MB008.namprd05.prod.outlook.com;CLIP:129.108.37.93;FPR:3ADEF03E.9E339C09.87D2589.84EF8869.201A3;InfoNoRecordsA:0;MX:1;LANG:en; } } 6, 29 -- Content-Type: text/plain; charset="Windows-1252" } } 6, 29 -- MIME-Version: 1.0 } } 6, 29 -- X-OriginatorOrg: miners.utep.edu } } 6, 29 -- Content-Transfer-Encoding: 8bit } } 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172HCnn004797 } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 10, 25 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Feb 7 02:01:34 2014 } 10, 25 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1781YdX013671 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2014 02:01:34 -0600 } 10, 25 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 10, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id 51B6224238B; } 10, 25 -- Fri, 7 Feb 2014 09:01:52 +0100 (CET) } 10, 25 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr } 10, 25 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) } 10, 25 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) } 10, 25 -- with ESMTP id 458afHncdtNz; Fri, 7 Feb 2014 09:01:51 +0100 (CET) } 10, 25 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) } 10, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id 3652524236C; } 10, 25 -- Fri, 7 Feb 2014 09:01:51 +0100 (CET) } 10, 25 -- Message-ID: {52F492D2.2060601-at-ipcms.u-strasbg.fr} } 10, 25 -- Date: Fri, 07 Feb 2014 09:01:22 +0100 } 10, 25 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } 10, 25 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:17.0) Gecko/20130330 Thunderbird/17.0.5 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: ahinojos2-at-miners.utep.edu, microscopy-at-microscopy.com } 10, 25 -- Subject: Re: [Microscopy] TEM Acquisition Help } 10, 25 -- References: {201402070228.s172SkD0020695-at-ns.microscopy.com} } 10, 25 -- In-Reply-To: {201402070228.s172SkD0020695-at-ns.microscopy.com} } 10, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 26 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Feb 12 02:22:45 2014 11, 26 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C8MjmP027877 11, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 02:22:45 -0600 11, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 11, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id 54AEC242352 11, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 09:23:14 +0100 (CET) 11, 26 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr 11, 26 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) 11, 26 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) 11, 26 -- with ESMTP id tlzUJTuQtmiX for {microscopy-at-microscopy.com} ; 11, 26 -- Wed, 12 Feb 2014 09:23:13 +0100 (CET) 11, 26 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 11, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id 0BEA524230E 11, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 09:23:13 +0100 (CET) 11, 26 -- Message-ID: {52FB2F5F.6010906-at-ipcms.u-strasbg.fr} 11, 26 -- Date: Wed, 12 Feb 2014 09:22:55 +0100 11, 26 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 11, 26 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:17.0) Gecko/20130330 Thunderbird/17.0.5 11, 26 -- MIME-Version: 1.0 11, 26 -- To: microscopy-at-microscopy.com 11, 26 -- Subject: TEM Acquisition Help : some precisions 11, 26 -- References: {201402070812.s178CgaH028148-at-ns.microscopy.com} 11, 26 -- In-Reply-To: {201402070812.s178CgaH028148-at-ns.microscopy.com} 11, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 26 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
First, is the "Panel Dim" knob pushed in? Turns on the panel. "Data Dim" has to be up to be able to see anything on the monitor. There is a delay while the computer runs self tests. Power supply, left half: The 4 boxes in the top row should all have green lights on when the 'scope is on, regardless of whether or not the panel is on. Same for the 30V "dbl" box in the middle row, and the large black box in the bottom. When the HT button is pushed, the 24V filament box green light will come on in the middle row, as well as the left-most box in that row (no label on our CM-10). There should be no red lights - check the 2 right-most boxes in the middle row. Those are safeties, and have reset buttons. If those are showing red lights, turn off the HT and push the reset buttons.
But. The column shouldn't be making any noise at all. Is it really electronic, or is it a crackly water-flowing noise? Could be air in the cooling lines, which is likely if the 'scope was torn apart. The water lines would need to be disconnected at the exits, and water run through to remove any air in the lines. Were the water lines cleaned with H2O2 or CLR (brand) cleaner? If not, they may be blocked by crud or corrosion. 1 liter 3% H2O2 in the chiller tank for 2 hr - overnight, then 4 or 5 flushes with clean water should clear them.
If it is an electronic noise ... check the connections. If they're OK, then arg. Problem. Get out the sacrificial rooster or undergrad and ...
Phil
} Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10 troubleshooting } } Message: Upon first turning our newly renovated Philips CM10 TEM, we cannot get any of the functions } to work. The standby light on the power supply unit is illuminated as well as the ON button by the } display monitor on the electronics rack. When the ON button by the monitor is pressed, the column } makes a sort of electronic noise. Other than that there seems to be no power getting to the monitor } or any controls. Any idea on what our problem may be? Are there any controls on the power supply } that need to be turned on other than the MAINS dial? -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 29 -- From oshel1pe-at-cmich.edu Wed Feb 12 07:33:01 2014 6, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1CDX1DU005650 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 07:33:01 -0600 6, 29 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 6, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1CDWjpd002219; 6, 29 -- Wed, 12 Feb 2014 08:32:57 -0500 6, 29 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 6, 29 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 6, 29 -- 14.3.174.1; Wed, 12 Feb 2014 08:32:45 -0500 6, 29 -- Message-ID: {52FB77FE.4070107-at-cmich.edu} 6, 29 -- Date: Wed, 12 Feb 2014 08:32:46 -0500 6, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 29 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 29 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 29 -- MIME-Version: 1.0 6, 29 -- To: {jcsmtf-at-mail.missouri.edu} , micro {Microscopy-at-microscopy.com} 6, 29 -- Subject: Re: [Microscopy] viaWWW:CM10 troubleshooting 6, 29 -- References: {201402120102.s1C12SQr030082-at-ns.microscopy.com} 6, 29 -- In-Reply-To: {201402120102.s1C12SQr030082-at-ns.microscopy.com} 6, 29 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- X-Originating-IP: [141.209.160.122] 6, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 29 -- X-Spam-Score: -0.50 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 6, 29 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 29 -- X-CanItPRO-Stream: default 6, 29 -- X-Canit-Stats-ID: 02LppwVdQ - 00d20a283f78 - 20140212 6, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
The Biozentrum of the University of Basel is one of the leading institutes worldwide for molecular and biomedical basic research and teaching. It is home to more than 30 research groups with scientists from over 40 countries. Research at the Biozentrum focuses on the areas of Cell Growth & Development, Infection Biology, Neurobiology, Structural Biology & Biophysics and Computational & Systems Biology. With its more than 550 employees, the Biozentrum is the largest department at the University of Basel’s Faculty of Science.
The University of Basel maintains two sites for electron microscopy, the C-CINA and the ZMB. The Center for Cellular Imaging and NanoAnalytics (C-CINA) performs advanced electron microscopy research in life sciences, see http://c-cina.org. The ZMB provides TEM and SEM services to the University, see http://zmb.unibas.ch. The C-CINA and the ZMB together operate 15 electron microscopes including a FEI Titan Krios with a Gatan K2 Summit direct electron detector, FEI Polara, CM200FEG, Helios FIB-SEM and a Quanta200FEG with a Gatan 3View.
We have an opening for an
========================= EM Service Engineer =========================
Your responsibilities will include the maintenance and repair of all electron microscopes of C-CINA and the ZMB. We do not have any factory maintenance contracts but may request additional help from the factory when needed. Responsibilities of this position are maintenance and repair of the electron microscopes including; problem diagnosis, ordering replacement parts from the factory, and repair. This includes: the Titan Krios Autoloader, cryo-stages, FEG and high voltage supplies, vacuum systems, mechanics, electronics and software backups.
In addition, upon interest, this engineer may participate in electron microscope hardware development projects, which involve hardware design, fabrication in collaboration with our mechanical workshop, construction, and development of electronic control in collaboration with an electronic workshop. Participation in research projects concerning structural biology data collection with these instruments is also possible. C-CINA is a University research group; we develop instruments and methods, and apply them to study the high-resolution 3D structure of biological specimens.
Salary and living conditions in Basel are attractive. The lab language is English.
Your profile Experience in EM service for TEM, ideally also for SEM and FIB. Hardware repair experience.
We offer This is a full-time position permanently funded by the Rektorat of the University of Basel. A highly sought after position in one of the most beautiful countries in Europe. We offer a welcoming working environment in an international and multidisciplinary group covering biochemistry, electron microscopy, structural biology, and data analysis. We are an equal opportunity employer.
Basel is an international city with people from 150 nations. Located at the border where three countries meet - Switzerland-Germany-France. It is Europe’s most important life sciences hub. Basel provides a high standard of living and a rich and varied cultural atmosphere.
For further information, please contact (ABSOLUTE CONFIDENTIALITY IS ASSURED):
Henning Stahlberg, Director Center for Cellular Imaging and NanoAnalytics (C-CINA) Prof. for Structural Biology and Biophysics, Biozentrum, WRO-1058, Mattenstrasse 26 University of Basel, CH-4058 Basel, Switzerland Tel: +41-61-387 32 62 ; http://c-cina.org mailto:Henning.Stahlberg-at-unibas.ch
==============================Original Headers============================== 12, 37 -- From henning.stahlberg-at-unibas.ch Thu Feb 13 04:08:03 2014 12, 37 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 12, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DA82HN011000 12, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 04:08:02 -0600 12, 37 -- Received: from localhost (localhost [127.0.0.1]) 12, 37 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 76E002200EF 12, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 11:08:01 +0100 (CET) 12, 37 -- X-Virus-Scanned: amavisd-new at unibas.ch 12, 37 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 12, 37 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 12, 37 -- with LMTP id 3DgBK0BQ6eNJ for {Microscopy-at-microscopy.com} ; 12, 37 -- Thu, 13 Feb 2014 11:08:01 +0100 (CET) 12, 37 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131]) 12, 37 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 6507E220130 12, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 11:08:01 +0100 (CET) 12, 37 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 12, 37 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id 12, 37 -- 14.03.0174.001; Thu, 13 Feb 2014 11:08:01 +0100 12, 37 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 12, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 37 -- Subject: EM Service Engineer Position available at University Basel, 12, 37 -- Switzerland 12, 37 -- Thread-Topic: EM Service Engineer Position available at University Basel, 12, 37 -- Switzerland 12, 37 -- Thread-Index: AQHPKKN5Os4QjMCcZ0uwm3RcNz3MpQ== 12, 37 -- Date: Thu, 13 Feb 2014 10:08:00 +0000 12, 37 -- Message-ID: {740E1207-B098-4057-A0F4-508C03606617-at-unibas.ch} 12, 37 -- Accept-Language: en-US, de-CH 12, 37 -- Content-Language: en-US 12, 37 -- X-MS-Has-Attach: 12, 37 -- X-MS-TNEF-Correlator: 12, 37 -- x-originating-ip: [129.132.128.85] 12, 37 -- Content-Type: text/plain; charset="Windows-1252" 12, 37 -- Content-ID: {E08389ABE9116644A71EF480521ED231-at-zuv.ads.unibas.ch} 12, 37 -- MIME-Version: 1.0 12, 37 -- Content-Transfer-Encoding: 8bit 12, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1DA82HN011000 ==============================End of - Headers==============================
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Email: mamiller-at-coh.org Name: Marcia M. Miller
Organization: Beckman Research Institute, City of Hope
Title-Subject: [Filtered] Using agar to secure cell pellets
Message: The agar that we are currently using to secure cell pellets is causing a really annoying background all over the sections both inside and outside of cells. This is a terrible graininess that we have seen now in several preps. What we have is bacterial grand from Applichem. We are pretty sure this is the cause of the graininess. Can anyone recommend another agar or another means of securing cell pellets? Sometimes users of our core can provide only small quantities of cells. Thanks in advance for suggestions. Best wishes, Marcia
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We have been using the low-melting-point agarose (NOT agar). It works great. You can find it at Sigma (Cat# A9414).
Zhaojie
Zhaojie Zhang, Ph. D. Director, Jenkins Microscopy Facility University of Wyoming Laramie, WY 82071 PHONE: 307-766-3038 FAX: 307-766-5625
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, February 13, 2014 7:06 AM To: Z.J. Zhang
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Email: mamiller-at-coh.org Name: Marcia M. Miller
Organization: Beckman Research Institute, City of Hope
Title-Subject: [Filtered] Using agar to secure cell pellets
Message: The agar that we are currently using to secure cell pellets is causing a really annoying background all over the sections both inside and outside of cells. This is a terrible graininess that we have seen now in several preps. What we have is bacterial grand from Applichem. We are pretty sure this is the cause of the graininess. Can anyone recommend another agar or another means of securing cell pellets? Sometimes users of our core can provide only small quantities of cells. Thanks in advance for suggestions. Best wishes, Marcia
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 13 08:00:01 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DE00WQ005497 14, 28 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 08:00:00 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 0ACC16BC390 14, 28 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 08:00:00 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id zEptrrs71ss4 for {microscopy-at-microscopy.com} ; 14, 28 -- Thu, 13 Feb 2014 07:59:50 -0600 (CST) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id C55976BC37E 14, 28 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 07:59:50 -0600 (CST) 14, 28 -- Message-ID: {52FCCFD6.10003-at-microscopy.com} 14, 28 -- Date: Thu, 13 Feb 2014 07:59:50 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Using agar to secure cell pellets 14, 28 -- References: {201402122132.s1CLWDdE000365-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201402122132.s1CLWDdE000365-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201402122132.s1CLWDdE000365-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 30, 31 -- From ZZhang-at-uwyo.edu Thu Feb 13 09:20:04 2014 30, 31 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2lp0207.outbound.protection.outlook.com [207.46.163.207]) 30, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DFK4Rb025690 30, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 09:20:04 -0600 30, 31 -- Received: from BN1PR05MB059.namprd05.prod.outlook.com (10.255.202.149) by 30, 31 -- BN1PR05MB059.namprd05.prod.outlook.com (10.255.202.149) with Microsoft SMTP 30, 31 -- Server (TLS) id 15.0.878.16; Thu, 13 Feb 2014 15:19:50 +0000 30, 31 -- Received: from BN1PR05MB059.namprd05.prod.outlook.com ([169.254.11.248]) by 30, 31 -- BN1PR05MB059.namprd05.prod.outlook.com ([169.254.11.248]) with mapi id 30, 31 -- 15.00.0878.008; Thu, 13 Feb 2014 15:19:50 +0000 30, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 30, 31 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 30, 31 -- Subject: RE: [Microscopy] viaWWW:Using agar to secure cell pellets 30, 31 -- Thread-Topic: [Microscopy] viaWWW:Using agar to secure cell pellets 30, 31 -- Thread-Index: AQHPKMTc2eiFVCauLUytXVEz7hk3XpqzShUQ 30, 31 -- Date: Thu, 13 Feb 2014 15:19:49 +0000 30, 31 -- Message-ID: {3d80287025ba4b2ea88c470fbc80541b-at-BN1PR05MB059.namprd05.prod.outlook.com} 30, 31 -- References: {201402131405.s1DE5YX5010524-at-ns.microscopy.com} 30, 31 -- In-Reply-To: {201402131405.s1DE5YX5010524-at-ns.microscopy.com} 30, 31 -- Accept-Language: en-US 30, 31 -- Content-Language: en-US 30, 31 -- X-MS-Has-Attach: 30, 31 -- X-MS-TNEF-Correlator: 30, 31 -- x-originating-ip: [129.72.151.168] 30, 31 -- x-forefront-prvs: 0121F24F22 30, 31 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(51874003)(252514010)(64544002)(199002)(189002)(13464003)(377454003)(63696002)(86362001)(81816001)(33646001)(66066001)(65816001)(80022001)(94946001)(93516002)(79102001)(95416001)(19580405001)(74662001)(74316001)(81686001)(87936001)(74366001)(2656002)(69226001)(93136001)(95666001)(15202345003)(56816005)(87266001)(90146001)(77096001)(15975445006)(80976001)(31966008)(77982001)(94316002)(59766001)(83322001)(47446002)(74502001)(81342001)(85306002)(81542001)(92566001)(85852003)(46102001)(51856001)(53806001)(74876001)(75432001)(49866001)(47736001)(76786001)(4396001)(76576001)(83072002)(15395725003)(47976001)(76796001)(50986001)(19580395003)(54356001)(56776001)(54316002)(74706001)(76482001)(24736002)(80792004)(491001);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR05MB059;H:BN1PR05MB059.namprd05.prod.outlook.com;CLIP:129.72.151.168;FPR:649EF5B5.ACC2913D.3AF1ADBD.40E1A801.2049A;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 30, 31 -- Content-Type: text/plain; charset="us-ascii" 30, 31 -- MIME-Version: 1.0 30, 31 -- X-OriginatorOrg: uwyo.edu 30, 31 -- Content-Transfer-Encoding: 8bit 30, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1DFK4Rb025690 ==============================End of - Headers==============================
Marcia, We have encase small fragile objects between Formvar films on a wire loop. Although this does require appropriate obeissance to the Formvar Spirits, the outcome in terms of structure preservation is excellent. You can read about it in Wu et al. 2012 Nature Protocols 7: 1113- 1124. (I can send you a pdf off line if you are interested). That paper describes using the method on a small plant root but there is no reason in principle why it would not work for a cell pellet. Probably you won't want to switch horses like this but sending a long the info in case you are interested. Good luck! Tobias
} l } ---------------------------------------------------------------------------- } } X-from: mamiller-at-coh.org () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mamiller-at-coh.org as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: mamiller-at-coh.org } Name: Marcia M. Miller } } Organization: Beckman Research Institute, City of Hope } } Title-Subject: [Filtered] Using agar to secure cell pellets } } Message: The agar that we are currently using to secure cell pellets } is causing a really annoying } background all over the sections both inside and outside of cells. } This is a terrible graininess } that we have seen now in several preps. What we have is bacterial } grand from Applichem. We are } pretty sure this is the cause of the graininess. Can anyone } recommend another agar or another means } of securing cell pellets? Sometimes users of our core can provide } only small quantities of cells. } Thanks in advance for suggestions. Best wishes, Marcia } } Login Host: 151.152.101.44 } Listserver Email Form V - 20120416 } ---------------------------------------------------------------------------
-- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Professor /_ / __ /__ \ \ \__ Biology Department / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / ___ / \ \__/ \ ____ Amherst, MA, 01003 www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 4, 21 -- From baskin-at-bio.umass.edu Thu Feb 13 09:28:29 2014 4, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DFSS9V001391 4, 21 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 09:28:29 -0600 4, 21 -- Received: from [172.30.52.170] (neutopia.bio.umass.edu [128.119.55.8]) 4, 21 -- (authenticated bits=0) 4, 21 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id s1DFSP7H007665 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 21 -- Thu, 13 Feb 2014 10:28:26 -0500 (EST) 4, 21 -- Mime-Version: 1.0 4, 21 -- Message-Id: {p06240505cf22939825da-at-[172.30.52.170]} 4, 21 -- In-Reply-To: {201402131400.s1DE0atb006103-at-ns.microscopy.com} 4, 21 -- References: {201402131400.s1DE0atb006103-at-ns.microscopy.com} 4, 21 -- Date: Thu, 13 Feb 2014 10:28:24 -0500 4, 21 -- To: microscopy-at-microscopy.com 4, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 4, 21 -- Subject: Re: [Microscopy] viaWWW:Using agar to secure cell pellets 4, 21 -- Cc: mamiller-at-coh.org 4, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Thu, 13 Feb 2014 10:28:26 -0500 (EST) 4, 21 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
We use 1.5% agarose in PBS (I assume other medium works too). We first mix the agarose with PBS in an eppendorf tube, then immerse the tube in boiling water for a few minutes. The agarose would be completed 'melted' at this point. We then transfer the tube to a 37 degree C water bath, to let it cool down to 37C. The agarose (melting point 36C) will stay melted at 37C.
We then mixed 1 part of cells and 1 part of agarose at 37C, then bring the mixture to room temperature. The mixture will become solid right away (no need for ice)!
Hope this helps.
Zhaojie
X-from: Erin Tranfield [mailto:etranfield-at-igc.gulbenkian.pt] Sent: Thursday, February 13, 2014 8:57 AM To: Z.J. Zhang
Hi Marcia:
We have been using the low-melting-point agarose (NOT agar). It works great. You can find it at Sigma (Cat# A9414).
Zhaojie
Zhaojie Zhang, Ph. D. Director, Jenkins Microscopy Facility University of Wyoming Laramie, WY 82071 PHONE: 307-766-3038 FAX: 307-766-5625
==============================Original Headers============================== 30, 33 -- From ZZhang-at-uwyo.edu Thu Feb 13 10:15:00 2014 30, 33 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2lp0206.outbound.protection.outlook.com [207.46.163.206]) 30, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DGF0LG031702 30, 33 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 10:15:00 -0600 30, 33 -- Received: from BN1PR05MB059.namprd05.prod.outlook.com (10.255.202.149) by 30, 33 -- BN1PR05MB060.namprd05.prod.outlook.com (10.255.202.153) with Microsoft SMTP 30, 33 -- Server (TLS) id 15.0.868.8; Thu, 13 Feb 2014 16:14:46 +0000 30, 33 -- Received: from BN1PR05MB059.namprd05.prod.outlook.com ([169.254.11.248]) by 30, 33 -- BN1PR05MB059.namprd05.prod.outlook.com ([169.254.11.248]) with mapi id 30, 33 -- 15.00.0878.008; Thu, 13 Feb 2014 16:14:46 +0000 30, 33 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 30, 33 -- To: "'Erin Tranfield'" {etranfield-at-igc.gulbenkian.pt} 30, 33 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 30, 33 -- Subject: RE: Guidance with LMP Agarose and Cell Pellets? 30, 33 -- Thread-Topic: Guidance with LMP Agarose and Cell Pellets? 30, 33 -- Thread-Index: AQHPKNRY0HFH/s8gp0C7z9/Nv0rptpqzW6bQ 30, 33 -- Date: Thu, 13 Feb 2014 16:14:45 +0000 30, 33 -- Message-ID: {95688f6dd3dd453d9a662c699413d7c6-at-BN1PR05MB059.namprd05.prod.outlook.com} 30, 33 -- References: {201402131537.s1DFbGp9021382-at-ns.microscopy.com} 30, 33 -- {AF9E7A88-93B4-453B-806D-9938EDC5F521-at-igc.gulbenkian.pt} 30, 33 -- In-Reply-To: {AF9E7A88-93B4-453B-806D-9938EDC5F521-at-igc.gulbenkian.pt} 30, 33 -- Accept-Language: en-US 30, 33 -- Content-Language: en-US 30, 33 -- X-MS-Has-Attach: 30, 33 -- X-MS-TNEF-Correlator: 30, 33 -- x-originating-ip: [129.72.151.168] 30, 33 -- x-forefront-prvs: 0121F24F22 30, 33 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(252514010)(48214007)(377454003)(129404003)(199002)(189002)(24454002)(81686001)(80976001)(74316001)(76786001)(76796001)(69226001)(74876001)(74706001)(74366001)(56776001)(87266001)(54316002)(76482001)(85852003)(2656002)(4396001)(94946001)(46102001)(83072002)(81342001)(66066001)(76576001)(85306002)(81542001)(87936001)(80022001)(95666001)(65816001)(81816001)(93516002)(77096001)(74662001)(86362001)(47446002)(15975445006)(31966008)(47736001)(92566001)(49866001)(15202345003)(63696002)(90146001)(59766001)(56816005)(77982001)(83322001)(47976001)(50986001)(79102001)(53806001)(19580395003)(94316002)(19580405001)(54356001)(95416001)(51856001)(33646001)(74502001)(93136001)(75432001)(24736002)(80792004);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR05MB060;H:BN1PR05MB059.namprd05.prod.outlook.com;CLIP:129.72.151.168;FPR:E45EF58D.A62AA7CD.39F91D97.C0F5CC3B.20440;InfoNoRecordsA:1;MX:1;LANG:en; 30, 33 -- Content-Type: text/plain; charset="iso-8859-1" 30, 33 -- MIME-Version: 1.0 30, 33 -- X-OriginatorOrg: uwyo.edu 30, 33 -- Content-Transfer-Encoding: 8bit 30, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1DGF0LG031702 ==============================End of - Headers==============================
Dear All, here are some images from a Raspberry leaf: http://www.electronmicroscopy.info/sem_images/index.htm?1 Can someone please help me identify the structures seen in the mid and right image? Especially those with the small "spheres" on the surface? Is this mostly mold on the surface?
Best wishes, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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realname - Anne Howson Email - AKHPhoto-at-pacbell.net ORGANIZATION - Richardson Bay Audubon Center EDUCATION - Graduate College LOCATION - Tiburon, Ca. QUESTION - We have an Olympus SZ61 Microscope with an Hitachi CCD color camera (model kP-D20BU attached which we connect to a projector to teach or docents and trainees about plankton in San Francisco Bay. Several months ago the system stopped projecting color images and will now only project black & white. This is not nearly as effective and dramatic. Can you suggest what might be wrong? We tried buying a new projector, but that did not fix the problem. Thanks for any help you can give us. As a small non-profit educational facility, we do not have much $.
==============================Original Headers============================== 2, 27 -- From oshel1pe-at-cmich.edu Thu Feb 13 15:44:37 2014 2, 27 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DLiafg009363 2, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 15:44:36 -0600 2, 27 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75] (may be forged)) 2, 27 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1DLbRtm003872 2, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 16:37:32 -0500 2, 27 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 2, 27 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 2, 27 -- 14.3.174.1; Thu, 13 Feb 2014 16:11:48 -0500 2, 27 -- Message-ID: {52FD3512.3080000-at-cmich.edu} 2, 27 -- Date: Thu, 13 Feb 2014 16:11:46 -0500 2, 27 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 2, 27 -- Reply-To: {oshel1pe-at-cmich.edu} 2, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 2, 27 -- MIME-Version: 1.0 2, 27 -- To: micro {Microscopy-at-microscopy.com} 2, 27 -- Subject: Ask-A-Microscopist Problems with Olympus CCD camera on light microscope 2, 27 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 2, 27 -- Content-Transfer-Encoding: 7bit 2, 27 -- X-Originating-IP: [141.209.160.122] 2, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 27 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 2, 27 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 27 -- X-CanItPRO-Stream: default 2, 27 -- X-Canit-Stats-ID: 05LpVBwMn - 10ee1c4fa5f8 - 20140213 2, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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You should be able to get excellent in-person help from the San Francisco Microscopical Society, http://sfmicrosoc.org They're a mix of professional and amateur microscopists in the SF Bay area who are dedicated to the sort of educational outreach that you're doing. -- Caroline Schooley Project MICRO Co-Chair Microscopy Society of America 45301 Caspar Point Road, Box 117 Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/education/projectMICRO
==============================Original Headers============================== 2, 19 -- From schooley-at-mcn.org Thu Feb 13 16:35:19 2014 2, 19 -- Received: from smtp3.mcn.org (smtp3.mcn.org [216.150.240.89]) 2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DMZJY1028772 2, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 16:35:19 -0600 2, 19 -- Received: from [66.81.52.152] 2, 19 -- by smtp3.mcn.org with esmtpa (Exim 4.82) 2, 19 -- (envelope-from {schooley-at-mcn.org} ) 2, 19 -- id 1WE4sF-00062H-5v; Thu, 13 Feb 2014 14:35:18 -0800 2, 19 -- Mime-Version: 1.0 2, 19 -- Message-Id: {a06240801cf22f6bea43a-at-[66.81.52.152]} 2, 19 -- In-Reply-To: {201402132153.s1DLreFE018372-at-ns.microscopy.com} 2, 19 -- References: {201402132153.s1DLreFE018372-at-ns.microscopy.com} 2, 19 -- Date: Thu, 13 Feb 2014 14:35:06 -0800 2, 19 -- To: Anne Howson {AKHPhoto-at-pacbell.net} 2, 19 -- From: Caroline Schooley {schooley-at-mcn.org} 2, 19 -- Subject: Re: [Microscopy] Ask-A-Microscopist Problems with Olympus CCD 2, 19 -- camera on light microscope 2, 19 -- Cc: Microscopy-at-microscopy.com 2, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Hello All, Please, could anybody give us an advice or hint on following problem? We have a trouble with our Philips CM100. I can see the light on main screen but cannot focus the beam with Intensity button. At both end positions of Intensity button range I can hear the beeps. Microscope restart did not help. Without any touching the Intensity button C2 Condenser current in LM mode is oscillating between 3900 mA and 3924 mA. In M mode it is oscillating from 3868 mA to 3944 mA. C1 Condenser currents seem to be OK (389, 424, 470, 550, 640, 748, 855,1026, 1266, 1486, 2166 mA for spotsize from 1 to 11).
Thanking you in advance.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR. v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 4, 37 -- From benada-at-biomed.cas.cz Fri Feb 14 04:16:55 2014 4, 37 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1EAGtMe023859 4, 37 -- for {Microscopy-at-Microscopy.com} ; Fri, 14 Feb 2014 04:16:55 -0600 4, 37 -- X-ASG-Debug-ID: 1392373006-05011e01d2048a0001-FOsErg 4, 37 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id lLnCFVOo3ehWjmrK (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO) for {Microscopy-at-Microscopy.com} ; Fri, 14 Feb 2014 11:16:46 +0100 (CET) 4, 37 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 4, 37 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 4, 37 -- Received: from u117fs (c111kh.mbu.cas.cz [147.231.44.14]) 4, 37 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 4, 37 -- (No client certificate requested) 4, 37 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 60AEE2C8472 4, 37 -- for {Microscopy-at-Microscopy.com} ; Fri, 14 Feb 2014 11:16:46 +0100 (CET) 4, 37 -- Date: Fri, 14 Feb 2014 11:16:44 +0100 4, 37 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 4, 37 -- To: Microscopy-at-Microscopy.com 4, 37 -- Subject: CM100 C2 problem 4, 37 -- Message-ID: {20140214111644.36e0f807-at-u117fs} 4, 37 -- X-ASG-Orig-Subj: CM100 C2 problem 4, 37 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 4, 37 -- =?UTF-8?B?xIxS?= 4, 37 -- X-Mailer: Claws Mail 3.9.3 (GTK+ 2.24.10; i486-pc-linux-gnu) 4, 37 -- MIME-Version: 1.0 4, 37 -- Content-Type: text/plain; charset=US-ASCII 4, 37 -- Content-Transfer-Encoding: 7bit 4, 37 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 4, 37 -- X-Barracuda-Start-Time: 1392373006 4, 37 -- X-Barracuda-Encrypted: AES256-SHA 4, 37 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 4, 37 -- X-Barracuda-BRTS-Status: 1 4, 37 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 4, 37 -- X-Barracuda-Spam-Score: 0.00 4, 37 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 4, 37 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.145103 4, 37 -- Rule breakdown below 4, 37 -- pts rule name description 4, 37 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
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realname - David Glick Email - glickdavidb-at-gmail.com ORGANIZATION - San Joaquin Delta College EDUCATION - Undergraduate College LOCATION - Stockton Ca. USA SUBJECT_OF_QUESTION - Level of Education QUESTION - I am currently working towards a two year certificate in EM and considering continuing my education on a part-time basis. My question is two part: First; If I am considering working in the materials/crystalline sector how far can I expect to get with just a certificate? Second; Does obtaining a degree above a bachelors improve upward mobility in the industry? The Reason I ask these questions is that I am a 45 year old re-entry student and do not want to needlessly spend time and money in a university if it won't improve my employment standard.
==============================Original Headers============================== 4, 28 -- From oshel1pe-at-cmich.edu Fri Feb 14 07:02:23 2014 4, 28 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1ED2NE8012132 4, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 07:02:23 -0600 4, 28 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75] (may be forged)) 4, 28 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1ECsueu014469 4, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 07:55:19 -0500 4, 28 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 4, 28 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 28 -- 14.3.174.1; Fri, 14 Feb 2014 08:02:07 -0500 4, 28 -- Message-ID: {52FE13D7.2030406-at-cmich.edu} 4, 28 -- Date: Fri, 14 Feb 2014 08:02:15 -0500 4, 28 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 4, 28 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 28 -- MIME-Version: 1.0 4, 28 -- To: micro {Microscopy-at-microscopy.com} 4, 28 -- Subject: Ask-A-Microscopist How helpful are advanced degrees in getting microscopy 4, 28 -- jobs? 4, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 28 -- Content-Transfer-Encoding: 7bit 4, 28 -- X-Originating-IP: [141.209.160.122] 4, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 28 -- X-Spam-Score: 0.20 () [Hold at 6.00] RDNS_NONE,_L_IAA,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 28 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 28 -- X-CanItPRO-Stream: default 4, 28 -- X-Canit-Stats-ID: 05LqcTj86 - f70fc822a112 - 20140214 4, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
In my 40 years of chemistry and microscopy I've seen changes. Today industry hires BS to do the work technician did 30 years ago. 60 years ago you might have gotten an interview for a position as a technician but now you don't get in the door with out your sheep skin. I took my BS degree in Chemistry and scrambled to take microscopy courses at McCrone and other places. I found a position as a Microscopist and never looked back.
I worked for a man who had a PhD in the metallography of welding, He was moving up the management chain powdered by his drive and the PhD. So yes, I would say a degree is better than no degree. An advanced degree is better than a BS, but pick your field with care. Add other skill sets, like programming, electronics to round out your skill set.
Nothing is guarantied. I have a friend with a BS in chemical engineering and a masters in chemistry. He's in law school because he couldn't find a job in those fields. But he will not leave California either.......
If it was a perfect world, you could try to find a position which would allow you to finish school while employed. My limited experience is you then need to change job as soon as possible because management will always see you as a technician.
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Friday, February 14, 2014 8:12 AM To: Frank Karl
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realname - David Glick Email - glickdavidb-at-gmail.com ORGANIZATION - San Joaquin Delta College EDUCATION - Undergraduate College LOCATION - Stockton Ca. USA SUBJECT_OF_QUESTION - Level of Education QUESTION - I am currently working towards a two year certificate in EM and considering continuing my education on a part-time basis. My question is two part: First; If I am considering working in the materials/crystalline sector how far can I expect to get with just a certificate? Second; Does obtaining a degree above a bachelors improve upward mobility in the industry? The Reason I ask these questions is that I am a 45 year old re-entry student and do not want to needlessly spend time and money in a university if it won't improve my employment standard.
==============================Original Headers============================== 4, 28 -- From oshel1pe-at-cmich.edu Fri Feb 14 07:02:23 2014 4, 28 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1ED2NE8012132 4, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 07:02:23 -0600 4, 28 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75] (may be forged)) 4, 28 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1ECsueu014469 4, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 07:55:19 -0500 4, 28 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 4, 28 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 28 -- 14.3.174.1; Fri, 14 Feb 2014 08:02:07 -0500 4, 28 -- Message-ID: {52FE13D7.2030406-at-cmich.edu} 4, 28 -- Date: Fri, 14 Feb 2014 08:02:15 -0500 4, 28 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 4, 28 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 28 -- MIME-Version: 1.0 4, 28 -- To: micro {Microscopy-at-microscopy.com} 4, 28 -- Subject: Ask-A-Microscopist How helpful are advanced degrees in getting microscopy 4, 28 -- jobs? 4, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 28 -- Content-Transfer-Encoding: 7bit 4, 28 -- X-Originating-IP: [141.209.160.122] 4, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 28 -- X-Spam-Score: 0.20 () [Hold at 6.00] RDNS_NONE,_L_IAA,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 28 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 28 -- X-CanItPRO-Stream: default 4, 28 -- X-Canit-Stats-ID: 05LqcTj86 - f70fc822a112 - 20140214 4, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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==============================Original Headers============================== 19, 29 -- From frank_karl-at-ardl.com Fri Feb 14 08:50:01 2014 19, 29 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1EEo1Tt032708 19, 29 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 08:50:01 -0600 19, 29 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 19, 29 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Fri, 14 19, 29 -- Feb 2014 09:50:00 -0500 19, 29 -- From: Frank Karl {frank_karl-at-ardl.com} 19, 29 -- To: "'glickdavidb-at-gmail.com'" {glickdavidb-at-gmail.com} 19, 29 -- CC: "Microscopy Listserver (microscopy-at-microscopy.com)" 19, 29 -- {microscopy-at-microscopy.com} 19, 29 -- Date: Fri, 14 Feb 2014 09:49:59 -0500 19, 29 -- Subject: RE: [Microscopy] Ask-A-Microscopist How helpful are advanced 19, 29 -- degrees in getting microscopy 19, 29 -- Thread-Topic: [Microscopy] Ask-A-Microscopist How helpful are advanced 19, 29 -- degrees in getting microscopy 19, 29 -- Thread-Index: Ac8phmvKTolTGrT9TkKDtZE6GyLKlgAC2IUg 19, 29 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BD05BDE5D-at-exchange2k7.ad.ardl.com} 19, 29 -- References: {201402141311.s1EDBgFX025803-at-ns.microscopy.com} 19, 29 -- In-Reply-To: {201402141311.s1EDBgFX025803-at-ns.microscopy.com} 19, 29 -- Accept-Language: en-US 19, 29 -- Content-Language: en-US 19, 29 -- X-MS-Has-Attach: 19, 29 -- X-MS-TNEF-Correlator: 19, 29 -- acceptlanguage: en-US 19, 29 -- Content-Type: text/plain; charset="us-ascii" 19, 29 -- MIME-Version: 1.0 19, 29 -- Content-Transfer-Encoding: 8bit 19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1EEo1Tt032708 ==============================End of - Headers==============================
I am trying to hone my understanding of diffraction in microscopy: particularly the slit diffraction analogy and how diffraction affects resolution in microscopy. 1. If you take a sub-resolution point source of light (GFP molecule) and image it through an objective, you get a central maximum and a series of concentric disks due to diffraction during image formation. Question- What is the source of the diffraction? There are no slit like openings that are less than the wavelength of the light. I am presuming this diffraction due light diffracting at the edges of the objective? (I could not find a good written/diagrammatic description of the issue- can someone point me to one?)
2. The separation of Airy disk central maxima as an explanation for resolution makes some intuitive sense, but is only taking account of the NA of the objective as if there are no other sources of diffraction other than the objective. This would be reasonable for a purified fluorescent molecule/protein attached to a coverslip. However, in cells, you now add many other sources of diffraction as light passes into and out of a cell. So would it be more correct to say that the calculated resolution is a maximal or optimal resolution? I am presuming that this is routinely not acheived in cell/tissue imaging? Do we have an estimate of how much resolution is lost by a molecule being in the middle of a cell (ie. what you get from a single superresolution activated molecule in PALM) as compared to a purified molecules in isolation? Presumably you can back calculate this from the actual diffraction pattern measured for single activated molecules in a cell vs. in isolation? Thanks- Dave
David Knecht, Ph.D. Professor and Head of Core Microscopy Facility Department of Molecular and Cell Biology U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 9, 27 -- From david.knecht-at-uconn.edu Fri Feb 14 09:22:32 2014 9, 27 -- Received: from exchange.uconn.edu (cashuba.grove.ad.uconn.edu [137.99.30.165]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1EFMWEM019670 9, 27 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 09:22:32 -0600 9, 27 -- Received: from CASHUBH.grove.ad.uconn.edu (137.99.203.212) by 9, 27 -- CashubA.grove.ad.uconn.edu (137.99.30.165) with Microsoft SMTP Server (TLS) 9, 27 -- id 14.3.174.1; Fri, 14 Feb 2014 10:22:31 -0500 9, 27 -- Received: from MAILD.grove.ad.uconn.edu ([169.254.5.33]) by 9, 27 -- CASHUBH.grove.ad.uconn.edu ([137.99.203.212]) with mapi id 14.03.0174.001; 9, 27 -- Fri, 14 Feb 2014 10:22:31 -0500 9, 27 -- From: "Knecht, David" {david.knecht-at-uconn.edu} 9, 27 -- To: microscopy microscopy {microscopy-at-microscopy.com} 9, 27 -- Subject: Microscopy basics- airy disks and fluorescence resolution 9, 27 -- Thread-Topic: Microscopy basics- airy disks and fluorescence resolution 9, 27 -- Thread-Index: AQHPKZiUBUJwaeBxdU6ZZQ55NOMqcA== 9, 27 -- Date: Fri, 14 Feb 2014 15:22:31 +0000 9, 27 -- Message-ID: {4B6EB19C-C087-43E5-B386-07302688F655-at-uconn.edu} 9, 27 -- Accept-Language: en-US 9, 27 -- Content-Language: en-US 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- x-originating-ip: [137.99.47.75] 9, 27 -- Content-Type: text/plain; charset="us-ascii" 9, 27 -- Content-ID: {0E90464930A8774AB4703B604722335D-at-ad.uconn.edu} 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1EFMWEM019670 ==============================End of - Headers==============================
1) Actually the Young’s slit experiment doesn’t require the width or the distance between the slits to be less than the wavelength of light. You can do it using a slide that you’ve put a thin black coating on (using model airplane paint for example) and then scratch it twice with a razor and shine a light through it. If you do your trigonometry, you’ll find out that if you put the slits really close (as in a few hundred microns or even a mm) you can see the diffraction pattern pretty clearly some feet away. In a microscope, you have apertures, and tubes bounding the light path and that is what is causing your diffraction. If you are operating at high NA then often the limiting aperture is the objective. You’ll get diffraction whenever the light path from one side of the aperture is on the order of one wavelength of light different than in the middle of the aperture, practically speaking, high mag.
2) Point resolution limits described by airy disks etc are really rules of thumb. As you note, the resolution limit is the smallest feature you can separate from your image, nothing more or less. Usually that is much less than the Airy resolution due to inhomogeneities in the sample, like you describe. On the other hand, theoretically, if you know EXACTLY the diffraction characteristics of your entire system, and your sample is composed of point sources, and you have infinite signal to noise, you can separate two different points that are infinitely close together. You can make a quick demonstration with Matlab or a similar program by plotting two Gaussians with FWHM of 100 units, and centroids separated by 1 unit, and then do a fit to extract the separation between the two centroids. You won’t have any trouble even though this scenario is resolving peaks far, far below the "resolution limit." As soon as you add even a little noise, you cannot make the fit work. As soon as you allow for any uncertainty in the FWHM or skewness of the peaks, then you also can’t get a good number from your fit. I encourage you to try this, actually, it is very informative just how little noise or uncertainty in the peak shapes is required to destroy your ability to resolve the two peaks, and if you play with it for a while will wind up deriving a practical resolution limit “law” of your own. Chances are you will wind up with a limit not far from the FWHM of the Gaussians. If you like programming you can do this with Airy disks and you’ll “reinvent” the Airy resolution limit. In a real microscope, you always have some aberrations, and your sample always has some inhomogeneities (otherwise why are you looking at it?). So the Airy resolution is a very practical limit and very useful, but there is no deep fundamental reason why the grand master of the universe says that’s the resolution limit. It’s just a good demarcation that was chosen based on a reasonable application of diffraction through an aperture.
I hope this helps!
Zack Gainsforth Space Sciences Laboratory, UC Berkeley 7 Gauss Way Berkeley, CA 94720 cell: 626-437-9186(tel://cell:%20626-437-9186) zackg-at-ssl.berkeley.edu(mailto:zackg-at-ssl.berkeley.edu)
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Message: Upon turning on our CM10 for the first time after the install there is a message saying "Pneumatics". I can't seem to get the ODP to turn on, and twice now the viewing glass has been pushed out by the compressed air. Does anyone have any ideas on what the problem may be?
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Message: I have not been able to activate the HIVAC and UHV on our CM10. The pressures of the pironi gauges are: P1=82, P2=19, P3=0, IGP=0. This is after the pre-vavc has been running for roughly 20 minutes. Once the pre-vac is turned off P2 goes to about ~70. Is it normal to take longer than this to get the P2 down below 13.3?
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 14 16:51:00 2014 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1EMp0Ri004534 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 16:51:00 -0600 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 76A9D6BD4AF 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 16:51:00 -0600 (CST) 11, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id rElzRzX1XjnU for {microscopy-at-microscopy.com} ; 11, 28 -- Fri, 14 Feb 2014 16:50:59 -0600 (CST) 11, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 2AC766BD4A2 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 16:50:59 -0600 (CST) 11, 28 -- Message-ID: {52FE9DD3.1080205-at-microscopy.com} 11, 28 -- Date: Fri, 14 Feb 2014 16:50:59 -0600 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:CM10 HIVAC 11, 28 -- References: {201402142006.s1EK6E8d000836-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201402142006.s1EK6E8d000836-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201402142006.s1EK6E8d000836-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A "pneumatics" error message generally means that your air pressure is too low to operate the valves of the microscope. The FEI TEMs generally want to have ~6 bar air pressure.
If your viewing window is being forced out, it sounds like you may have a more serious problem than the pneumatics error. The viewing chamber is not supposed to have a positive pressure but be under vacuum. Do you have a pressurized air supply hooked to the camera vent valve (valve V12 on the vacuum schematic)? It's possible that you have a leaky valve.
Cheers, Henk
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==============================Original Headers============================== 7, 47 -- From colijn.1-at-osu.edu Fri Feb 14 21:08:59 2014 7, 47 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1ehsobe005.messaging.microsoft.com [216.32.181.185]) 7, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1F38vET012741 7, 47 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 21:08:59 -0600 7, 47 -- Received: from mail108-ch1-R.bigfish.com (10.43.68.228) by 7, 47 -- CH1EHSOBE021.bigfish.com (10.43.70.78) with Microsoft SMTP Server id 7, 47 -- 14.1.225.22; Sat, 15 Feb 2014 03:08:56 +0000 7, 47 -- Received: from mail108-ch1 (localhost [127.0.0.1]) by 7, 47 -- mail108-ch1-R.bigfish.com (Postfix) with ESMTP id 9423024031B for 7, 47 -- {microscopy-at-microscopy.com} ; Sat, 15 Feb 2014 03:08:56 +0000 (UTC) 7, 47 -- X-Forefront-Antispam-Report: CIP:164.107.81.210;KIP:(null);UIP:(null);IPV:NLI;H:cio-krc-pf03;RD:none;EFVD:NLI 7, 47 -- X-SpamScore: 2 7, 47 -- X-BigFish: VPS2(zzbb2dI62a3I98dI9371I1432Izz1f42h2148h1d77h208ch1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch1fc6hzd9hz1de098h177df4h17326ah8275bh1de097h186068hz2fh2a8h839h947hd25he5bhf0ah1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh162dh1631h1758h1765h18e1h190ch1946h19b4h19c3h19ceh1ad9h1b0ah224fh1d0ch1d2eh1d3fh1dfeh1dffh1f5fh1fe8h1ff5h209eh22d0h2336h2438h2461h2476h2487h24d7h2516h2545h255eh1b1cn1b1bi1155h) 7, 47 -- Received-SPF: pass (mail108-ch1: domain of osu.edu designates 164.107.81.210 as permitted sender) client-ip=164.107.81.210; envelope-from=colijn.1-at-osu.edu; helo=cio-krc-pf03 ;cio-krc-pf03 ; 7, 47 -- Received: from mail108-ch1 (localhost.localdomain [127.0.0.1]) by mail108-ch1 7, 47 -- (MessageSwitch) id 1392433734796166_15640; Sat, 15 Feb 2014 03:08:54 +0000 7, 47 -- (UTC) 7, 47 -- Received: from CH1EHSMHS024.bigfish.com (snatpool1.int.messaging.microsoft.com 7, 47 -- [10.43.68.242]) by mail108-ch1.bigfish.com (Postfix) with ESMTP id 7, 47 -- B49BB1000DB; Sat, 15 Feb 2014 03:08:54 +0000 (UTC) 7, 47 -- Received: from cio-krc-pf03 (164.107.81.210) by CH1EHSMHS024.bigfish.com 7, 47 -- (10.43.70.24) with Microsoft SMTP Server (TLS) id 14.16.227.3; Sat, 15 Feb 7, 47 -- 2014 03:08:54 +0000 7, 47 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) (using TLSv1 7, 47 -- with cipher RC4-MD5 (128/128 bits)) (No client certificate requested) by 7, 47 -- cio-krc-pf03 (Postfix) with ESMTPS id 36C14C0051; Fri, 14 Feb 2014 22:08:54 7, 47 -- -0500 (EST) 7, 47 -- Received: from [192.168.1.102] (75.118.135.65) by smtp.service.osu.edu 7, 47 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.174.1; Fri, 14 Feb 7, 47 -- 2014 22:08:53 -0500 7, 47 -- Message-ID: {52FEDA43.4000002-at-osu.edu} 7, 47 -- Date: Fri, 14 Feb 2014 22:08:51 -0500 7, 47 -- From: Henk Colijn {colijn.1-at-osu.edu} 7, 47 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 7, 47 -- MIME-Version: 1.0 7, 47 -- To: {microscopy-at-microscopy.com} , {jcsmtf-at-mail.missouri.edu} 7, 47 -- Subject: Re: [Microscopy] viaWWW:CM10 Error Message 1 7, 47 -- References: {201402142252.s1EMqq4n007272-at-ns.microscopy.com} 7, 47 -- In-Reply-To: {201402142252.s1EMqq4n007272-at-ns.microscopy.com} 7, 47 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 7, 47 -- Content-Transfer-Encoding: 7bit 7, 47 -- X-Originating-IP: [75.118.135.65] 7, 47 -- X-CFilter-Loop: Forward 7, 47 -- X-FOPE-CRA-Verdict: 164.107.81.210$mail.missouri.edu%0%1%osu.edu%False%False%0$ 7, 47 -- X-OriginatorOrg: osu.edu 7, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 7, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%MAIL.MISSOURI.EDU$RO%1$TLS%0$FQDN%$TlsDn% ==============================End of - Headers==============================
Hi Josh, The ODP needs a heat up time of about 20 min. after the vacuum system has been off for a while. P3 = 0 means it is 100+. When the ODP is heated the vacuum system will continu by opening the valve between the ODP and the camera, then P3 will go down as well. Hans Janssen Netherlands Cancer Institute (NKI/AvL) Amsterdam
_________________________________ Van: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Verzonden: vrijdag 14 februari 2014 23:57 Aan: Hans Janssen Onderwerp: [Microscopy] viaWWW:CM10 HIVAC
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Message: I have not been able to activate the HIVAC and UHV on our CM10. The pressures of the pironi gauges are: P1=82, P2=19, P3=0, IGP=0. This is after the pre-vavc has been running for roughly 20 minutes. Once the pre-vac is turned off P2 goes to about ~70. Is it normal to take longer than this to get the P2 down below 13.3?
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==============================Original Headers============================== 16, 36 -- From j.janssen-at-nki.nl Sat Feb 15 08:18:23 2014 16, 36 -- Received: from out26-ams.mf.surf.net (out26-ams.mf.surf.net [145.0.1.26]) 16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1FEINZP014308 16, 36 -- for {Microscopy-at-Microscopy.com} ; Sat, 15 Feb 2014 08:18:23 -0600 16, 36 -- Received: from edge.nki.nl (edge-1.nki.nl [194.171.7.92]) 16, 36 -- by outgoing1-ams.mf.surf.net (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id s1FEILsf005440 16, 36 -- for {Microscopy-at-Microscopy.com} ; Sat, 15 Feb 2014 15:18:22 +0100 16, 36 -- Received: from caht-1.nki.nl (194.171.7.85) by edge-1.nki.nl (194.171.7.92) 16, 36 -- with Microsoft SMTP Server (TLS) id 8.3.279.1; Sat, 15 Feb 2014 15:18:21 16, 36 -- +0100 16, 36 -- Received: from mstr-1.nki.nl ([172.31.4.101]) by caht-1.nki.nl 16, 36 -- ([172.31.4.103]) with mapi; Sat, 15 Feb 2014 15:18:21 +0100 16, 36 -- From: {j.janssen-at-nki.nl} 16, 36 -- To: {Microscopy-at-Microscopy.com} 16, 36 -- Date: Sat, 15 Feb 2014 15:18:21 +0100 16, 36 -- Subject: RE: [Microscopy] viaWWW:CM10 HIVAC 16, 36 -- Thread-Topic: [Microscopy] viaWWW:CM10 HIVAC 16, 36 -- Thread-Index: Ac8p2DLQS5AiWiArTTSweX7bOOwqqAAf5Vd+ 16, 36 -- Message-ID: {F7BE8A0314FCF948BD9CFA10E54AEABD3261A5A66E-at-mstr-1.nki.nl} 16, 36 -- References: {201402142257.s1EMvqEj015958-at-ns.microscopy.com} 16, 36 -- In-Reply-To: {201402142257.s1EMvqEj015958-at-ns.microscopy.com} 16, 36 -- Accept-Language: nl-NL, en-US 16, 36 -- Content-Language: nl-NL 16, 36 -- X-MS-Has-Attach: 16, 36 -- X-MS-TNEF-Correlator: 16, 36 -- acceptlanguage: nl-NL, en-US 16, 36 -- Content-Type: text/plain; charset="us-ascii" 16, 36 -- MIME-Version: 1.0 16, 36 -- X-Bayes-Prob: 0.0001 (Score 0, tokens from: nki-out:default, base:default, -at--at-RPTN) 16, 36 -- X-Spam-Score: -0.50 () [Tag at 5.00] T_RP_MATCHES_RCVD:-0.01,CC(NL:-0.5) 16, 36 -- X-CanIt-Geo: ip=194.171.7.92; country=NL; region=07; city=Amsterdam; latitude=52.3500; longitude=4.9167; http://maps.google.com/maps?q=52.3500,4.9167&z=6 16, 36 -- X-CanItPRO-Stream: nki-out:default (inherits from nki:default,base:default) 16, 36 -- X-Canit-Stats-ID: 0uLqCilHy - 207119f90c29 - 20140215 (trained as not-spam) 16, 36 -- X-Scanned-By: CanIt (www . roaringpenguin . com) 16, 36 -- Content-Transfer-Encoding: 8bit 16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1FEINZP014308 ==============================End of - Headers==============================
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Email: rrw3q-at-virginia.edu Name: Mr Richard Robert White
Message: We are in need of the plug on a double tilt holder which plugs into a JEOL 2000FX TEM. If you have a broken double tilt holder, we could use the cable and plug from the holder.
Thanks,
Richard White University of Virginia rrw3q-at-virginia.edu
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Sun Feb 16 18:25:41 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1H0PfKU013171 16, 28 -- for {microscopy-at-microscopy.com} ; Sun, 16 Feb 2014 18:25:41 -0600 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id EF8E06BEE6F 16, 28 -- for {microscopy-at-microscopy.com} ; Sun, 16 Feb 2014 18:25:40 -0600 (CST) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id o1fbDy3zrPn2 for {microscopy-at-microscopy.com} ; 16, 28 -- Sun, 16 Feb 2014 18:25:36 -0600 (CST) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 740D86BEE5D 16, 28 -- for {microscopy-at-microscopy.com} ; Sun, 16 Feb 2014 18:25:36 -0600 (CST) 16, 28 -- Message-ID: {530156FF.70805-at-microscopy.com} 16, 28 -- Date: Sun, 16 Feb 2014 18:25:35 -0600 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:2000FX double tilt holder 16, 28 -- References: {201402162336.s1GNaJuE012713-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201402162336.s1GNaJuE012713-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201402162336.s1GNaJuE012713-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The "Pneumatics" error typically means your air pressure from the compresser to the valves is too low. Ours reads ~90 psi normally. Do you have any of the manuals? The appendix in the operator's manuals has the error codes. The ODP not coming on generally means insufficient cooling water, or the cooling water is too hot/too cold. It should be ~20-22 deg C (~70 F, if your Haskris cooler has the usual US temp gauge).
The viewing glass being pushed out means your vent pressure is high. Your column/camera/specimen airlock vent is hooked up to a dry nitrogen tank, yes? That should be at 1-2 psi, no more.
Re: your other email about pressures: I have not been able to activate the HIVAC and UHV on our CM10. The pressures of the pironi gauges are: P1=82, P2=19, P3=0, IGP=0. This is after the pre-vavc has been running for roughly 20 minutes. Once the pre-vac is turned off P2 goes to about ~70. Is it normal to take longer than this to get the P2 down below 13.3?
Our values in normal operation, after pumping overnight: P1 = 37 P2 = 74 P3 = 34 IGP = 14
Your P1 is high, which may mean a leak. If the column was taken apart, the leak is probably in one of those seals, and may not be visible under inspection. Clean. I've never seen a P2 value as low as yours. Seems wrong, likely connected with a leak, but I'd like a service engineer to explain it. P3 reads 0 either because the pressure is too high for it to come on, or the pressure is less than 34 - P3 goes to zero when the pressure drops below 34.
Also the P2 and/or P3 gauges may be malfunctioning. I'd assume a leak to start with. That's the usual bugaboo after taking apart an EM column.
Do you have an ion getter pump on your CM-10? If yes, disconnect it and *don't* run it until you get the rest of the vacuum system working (unplug connector X8, back of the column just below the IGP).
Phil
} Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10 Error Message } } Message: Upon turning on our CM10 for the first time after the install there is a message saying } "Pneumatics". I can't seem to get the ODP to turn on, and twice now the viewing glass has been } pushed out by the compressed air. Does anyone have any ideas on what the problem may be?
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 11, 31 -- From oshel1pe-at-cmich.edu Mon Feb 17 07:24:53 2014 11, 31 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1HDOqrE011385 11, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 07:24:53 -0600 11, 31 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 11, 31 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1HDOeYc015774; 11, 31 -- Mon, 17 Feb 2014 08:24:52 -0500 11, 31 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 11, 31 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 11, 31 -- 14.3.174.1; Mon, 17 Feb 2014 08:24:49 -0500 11, 31 -- Message-ID: {53020DA1.4050704-at-cmich.edu} 11, 31 -- Date: Mon, 17 Feb 2014 08:24:49 -0500 11, 31 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 11, 31 -- Reply-To: {oshel1pe-at-cmich.edu} 11, 31 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 11, 31 -- MIME-Version: 1.0 11, 31 -- To: {microscopylistserver-noreply-at-microscopy.com} , 11, 31 -- micro 11, 31 -- {Microscopy-at-microscopy.com} 11, 31 -- Subject: Re: [Microscopy] viaWWW:CM10 Error Message 1 11, 31 -- References: {201402142311.s1ENBVgx006752-at-ns.microscopy.com} 11, 31 -- In-Reply-To: {201402142311.s1ENBVgx006752-at-ns.microscopy.com} 11, 31 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 11, 31 -- Content-Transfer-Encoding: 7bit 11, 31 -- X-Originating-IP: [141.209.160.122] 11, 31 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 11, 31 -- X-Spam-Score: -0.50 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 11, 31 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 11, 31 -- X-CanItPRO-Stream: default 11, 31 -- X-Canit-Stats-ID: 02LrpoQPq - 3f88cc77f13c - 20140217 11, 31 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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realname - M Raja Email - plspce-at-amararaja.co.in ORGANIZATION - Amararaja Batteries Ltd EDUCATION - Undergraduate College LOCATION - Tirupathi, Andhra pradesh, India SUBJECT_OF_QUESTION - Color metallography-reg QUESTION - how to prepare etchent for lead base alloys and hardware & software requirement and also difficulties / limitations of color metallographic analysis. Actually i need details about benefits of color micrograph. Please revert back in above mail as soon as possible. Thank you in advance for your support.
==============================Original Headers============================== 3, 28 -- From oshel1pe-at-cmich.edu Mon Feb 17 07:38:45 2014 3, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1HDcj61024541 3, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 07:38:45 -0600 3, 28 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 3, 28 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1HDciP3017464 3, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 3, 28 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 3, 28 -- 14.3.174.1; Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- Message-ID: {530210E4.4040306-at-cmich.edu} 3, 28 -- Date: Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 3, 28 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 28 -- MIME-Version: 1.0 3, 28 -- To: micro {Microscopy-at-microscopy.com} 3, 28 -- Subject: Ask-A-Microscopist: etchant for lead alloys & any advantage to color 3, 28 -- imaging in metallography? 3, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 28 -- Content-Transfer-Encoding: 7bit 3, 28 -- X-Originating-IP: [141.209.160.122] 3, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 28 -- X-Spam-Score: 0.00 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,_L_SUPPORT,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 28 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 3, 28 -- X-CanItPRO-Stream: default 3, 28 -- X-Canit-Stats-ID: 02LrpCIWa - 649424478da2 - 20140217 3, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I'd try "Metallogtaphy: Principles and Practices by George F Vander Voot
It's an excellent book.
In some color staining systems, as I understand it, the physical orientation of the metal crystal results in a coloration that is constant for that orientation in that specific sample. Color photomicroscopy lets you image where these orientations are. Color provides contrast that simple gray scale may not.
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Monday, February 17, 2014 8:49 AM To: Frank Karl
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
realname - M Raja Email - plspce-at-amararaja.co.in ORGANIZATION - Amararaja Batteries Ltd EDUCATION - Undergraduate College LOCATION - Tirupathi, Andhra pradesh, India SUBJECT_OF_QUESTION - Color metallography-reg QUESTION - how to prepare etchent for lead base alloys and hardware & software requirement and also difficulties / limitations of color metallographic analysis. Actually i need details about benefits of color micrograph. Please revert back in above mail as soon as possible. Thank you in advance for your support.
==============================Original Headers============================== 3, 28 -- From oshel1pe-at-cmich.edu Mon Feb 17 07:38:45 2014 3, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1HDcj61024541 3, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 07:38:45 -0600 3, 28 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 3, 28 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1HDciP3017464 3, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 3, 28 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 3, 28 -- 14.3.174.1; Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- Message-ID: {530210E4.4040306-at-cmich.edu} 3, 28 -- Date: Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 3, 28 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 28 -- MIME-Version: 1.0 3, 28 -- To: micro {Microscopy-at-microscopy.com} 3, 28 -- Subject: Ask-A-Microscopist: etchant for lead alloys & any advantage to color 3, 28 -- imaging in metallography? 3, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 28 -- Content-Transfer-Encoding: 7bit 3, 28 -- X-Originating-IP: [141.209.160.122] 3, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 28 -- X-Spam-Score: 0.00 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,_L_SUPPORT,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 28 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 3, 28 -- X-CanItPRO-Stream: default 3, 28 -- X-Canit-Stats-ID: 02LrpCIWa - 649424478da2 - 20140217 3, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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==============================Original Headers============================== 15, 29 -- From frank_karl-at-ardl.com Mon Feb 17 09:32:27 2014 15, 29 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1HFWQPI018515 15, 29 -- for {microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 09:32:26 -0600 15, 29 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 15, 29 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 17 15, 29 -- Feb 2014 10:32:25 -0500 15, 29 -- From: Frank Karl {frank_karl-at-ardl.com} 15, 29 -- To: "'plspce-at-amararaja.co.in'" {plspce-at-amararaja.co.in} , 15, 29 -- "Microscopy 15, 29 -- Listserver (microscopy-at-microscopy.com)" {microscopy-at-microscopy.com} 15, 29 -- Date: Mon, 17 Feb 2014 10:32:25 -0500 15, 29 -- Subject: RE: [Microscopy] Ask-A-Microscopist: etchant for lead alloys & any 15, 29 -- advantage to color 15, 29 -- Thread-Topic: [Microscopy] Ask-A-Microscopist: etchant for lead alloys & any 15, 29 -- advantage to color 15, 29 -- Thread-Index: Ac8r5yfm+m0+puuoSgu3hRRq6Fv+pgADUywg 15, 29 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BD2D1B12C-at-exchange2k7.ad.ardl.com} 15, 29 -- References: {201402171349.s1HDnA2v011694-at-ns.microscopy.com} 15, 29 -- In-Reply-To: {201402171349.s1HDnA2v011694-at-ns.microscopy.com} 15, 29 -- Accept-Language: en-US 15, 29 -- Content-Language: en-US 15, 29 -- X-MS-Has-Attach: 15, 29 -- X-MS-TNEF-Correlator: 15, 29 -- acceptlanguage: en-US 15, 29 -- Content-Type: text/plain; charset="us-ascii" 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1HFWQPI018515 ==============================End of - Headers==============================
We've just updated our image processing instructions here at the NUANCE Center from Northwestern University.
These instructions are super easy to use and walk the user step-by-(sometimes painful) step to do all sorts of normal image processing procedures one might normally come across, from adjusting levels, to calculations, to some totally cheating techniques of drawing a mag line marker, to several different procedures to apply false color to an image.
THE EXTRA SPECIAL PART is the chapter on what I call Multi-Detector Color. Now I know I did not invent this technique, BUT I've worked out some pretty simple procedures that will allow you to make these really fantastic color images, even if you only have 1 SE detector in your SEM. It's totally free, so please check it out and let us know if you have corrections or suggestions.
I would appreciate hearing suggestions for a good mounting technique for imaging activated carbon and synthetic diamond particles (1-200 micron). When using standard conductive double sided adhesive carbon tabs on stubs, it is obviously quite challenging to get adequate contrast between the particles and the background (carbon tab).
Likewise, in doing particle size analysis it helps to not have touching particles and clumps. Any techniques that have been found to be successful would be interesting to hear.
Thank You!!
Mike Toalson Western Analytical Solutions, LLC o • 208.899.4425 m • 208.340.3895 w • www.materialanalyzers.com e • mike-at-materialanalyzers.com
==============================Original Headers============================== 9, 36 -- From mike-at-materialanalyzers.com Tue Feb 18 14:54:42 2014 9, 36 -- Received: from smtp106.biz.mail.ne1.yahoo.com (smtp106.biz.mail.ne1.yahoo.com [98.138.207.13]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id s1IKsgS8017426 9, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 14:54:42 -0600 9, 36 -- Received: (qmail 26061 invoked from network); 18 Feb 2014 20:54:41 -0000 9, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1392756881; bh=KL2fkLFHhxaw5G1tks+ZRF2GoZjrNk45IrZ0DhKP7Yg=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:From:To:Subject:Date:Organization:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language; b=Xpq18iCUznm1HOZ15Vc9lceTBiNd6C/kY8IUwM7EeTBfedjlacebVr09j9KYUXnFSbY/+Ivpyn5C8AahC/o+bz3ckjZTIIREP5quhyz8rVRxF0yd0Z4+ZNH6B+pPpKTog6gdw0gpPu8Ib/ne8O2KXgTmu14ULhCZJsq5/C6Yviw= 9, 36 -- X-Yahoo-Newman-Property: ymail-3 9, 36 -- X-YMail-OSG: vBrGDPsVM1lG8qIrqCO6h8tM2v7clDoP8_UWbFYJSm.LeU9 9, 36 -- kk8wiiDx3ogannCr3Py1MrVjH9tqIBqPq423NN8iG8a.Bq7.0GfAJkjXMUNl 9, 36 -- gQDBgKGrKXwwkMQZpr_ezoUcX8UAKafNI3A3Z9u56pVh30oMFZiYF8.yEpUX 9, 36 -- KKXkVgJaXFZd3KqDpZPmYbRVI6RZbxpBufFoo6s.Xss8CZIi8VHWqlReUtQs 9, 36 -- 949jqhP9gR.ABKtNdVKP03_Szp0LKjjKTIE60ArvkNuHjfQPclq.PpEqAL.g 9, 36 -- 34p66VV5MbTsNf2uXUmxyt6V7tD.oNhmLpNUAE8IPX8SODVrOqpMGaUIVe9V 9, 36 -- JbkYiVsacekAz16UQQqn5d1c6d9COyUCnMiRGkpZ_lQn71XIhED_R9gYtX0N 9, 36 -- kuIM1C3_uJBvduvlMacERDCKw.5gR90RH_LM7THDqdjzh_hoPSfAN._OHFZ8 9, 36 -- 9ugvnwxGyLZlI5uJMrTSFEeDrznqcWGpaMM2cI3STgat2Gd5xU3y_uTLIDEb 9, 36 -- LBAs4wT4GFVMs2s1Fo_ZDrAI6edHekL5Bq4Ewy6r4r4UmukAEzVUaZBw9jQo 9, 36 -- Zf4Hn8wOTxOTJ_7gNkT5K5fwWkN34nIetrmWuh.c6GGGsAduK8q.ID_dMVDU 9, 36 -- zOZV51jSmMp5PqXp5B2pkgD64gA-- 9, 36 -- X-Yahoo-SMTP: DCD965GswBCJIEnJvHD.h6b811gJzDyc5pf1kxJt 9, 36 -- X-Rocket-Received: from MikeLaptop (mike-at-24.119.142.15 with plain [98.138.105.25]) 9, 36 -- by smtp106.biz.mail.ne1.yahoo.com with SMTP; 18 Feb 2014 12:54:41 -0800 PST 9, 36 -- From: "Mike Toalson" {mike-at-materialanalyzers.com} 9, 36 -- To: {Microscopy-at-microscopy.com} 9, 36 -- Subject: SEM - Suggested mounting method for Carbon particles 9, 36 -- Date: Tue, 18 Feb 2014 13:54:30 -0700 9, 36 -- Organization: Western Analytical Solutions, LLC 9, 36 -- Message-ID: {027501cf2ceb$9f16ec10$dd44c430$-at-materialanalyzers.com} 9, 36 -- MIME-Version: 1.0 9, 36 -- Content-Type: text/plain; 9, 36 -- charset="iso-8859-1" 9, 36 -- X-Mailer: Microsoft Outlook 14.0 9, 36 -- Thread-Index: Ac8s6iyYCvomrZCeSeSlOzEyRqtHcw== 9, 36 -- Content-Language: en-us 9, 36 -- Content-Transfer-Encoding: 8bit 9, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IKsgS8017426 ==============================End of - Headers==============================
Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images.
Pete Eschbach Oregon State University
Sent from my iPhone
==============================Original Headers============================== 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 3, 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 3, 32 -- Subject: Montage software? 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 3, 32 -- Content-Type: text/plain; 3, 32 -- charset=us-ascii 3, 32 -- X-Mailer: iPhone Mail (11B554a) 3, 32 -- Message-Id: {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 3, 32 -- To: Microscopy-at-microscopy.com 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 3, 32 -- s=q20121106; t=1392761924; 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: 3, 32 -- Mime-Version; 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC 3, 32 -- vXeoTIY3Fw3YA== 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IMIi4s005720 ==============================End of - Headers==============================
The ImageJ plug-in MosaicJ is pretty easy to use and allows big images. I believe you need the TurboReg plug-in installed as well, as it can automatically stitch as long as you put them down close to the right position. I've used it successfully several times. Good Luck! Larry Scipioni ZS Genetics
-----Original Message----- X-from: peter.eschbach-at-comcast.net [mailto:peter.eschbach-at-comcast.net] Sent: Tuesday, February 18, 2014 5:30 PM To: LES-at-ZSGENETICS.COM
Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images.
Pete Eschbach Oregon State University
Sent from my iPhone
==============================Original Headers============================== 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 3, 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 3, 32 -- Subject: Montage software? 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 3, 32 -- Content-Type: text/plain; 3, 32 -- charset=us-ascii 3, 32 -- X-Mailer: iPhone Mail (11B554a) 3, 32 -- Message-Id: {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 3, 32 -- To: Microscopy-at-microscopy.com 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 3, 32 -- s=q20121106; t=1392761924; 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: 3, 32 -- Mime-Version; 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC 3, 32 -- vXeoTIY3Fw3YA== 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IMIi4s005720 ==============================End of - Headers==============================
==============================Original Headers============================== 10, 44 -- From les-at-zsgenetics.com Tue Feb 18 17:41:03 2014 10, 44 -- Received: from gateway01.websitewelcome.com ([74.52.222.242]) 10, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1INf3IB012377 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 17:41:03 -0600 10, 44 -- Received: by gateway01.websitewelcome.com (Postfix, from userid 5007) 10, 44 -- id D0AF25B3B6E87; Tue, 18 Feb 2014 17:41:02 -0600 (CST) 10, 44 -- Received: from gator2013.hostgator.com (gator2013.hostgator.com [50.87.144.13]) 10, 44 -- by gateway01.websitewelcome.com (Postfix) with ESMTP id 9D45D5B3B6DBB 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 17:41:02 -0600 (CST) 10, 44 -- Received: from [146.115.5.114] (port=63647 helo=LESZSG) 10, 44 -- by gator2013.hostgator.com with esmtpsa (TLSv1:AES128-SHA:128) 10, 44 -- (Exim 4.80) 10, 44 -- (envelope-from {les-at-zsgenetics.com} ) 10, 44 -- id 1WFuHe-00006Z-3V; Tue, 18 Feb 2014 17:41:02 -0600 10, 44 -- From: "Larry Scipioni" {les-at-zsgenetics.com} 10, 44 -- To: {peter.eschbach-at-comcast.net} 10, 44 -- Cc: {microscopy-at-microscopy.com} 10, 44 -- References: {201402182230.s1IMUCmo022411-at-ns.microscopy.com} 10, 44 -- In-Reply-To: {201402182230.s1IMUCmo022411-at-ns.microscopy.com} 10, 44 -- Subject: RE: [Microscopy] Montage software? 10, 44 -- Date: Tue, 18 Feb 2014 18:41:04 -0500 10, 44 -- Organization: ZS Genetics 10, 44 -- Message-ID: {00d201cf2d02$e407e1a0$ac17a4e0$-at-zsgenetics.com} 10, 44 -- MIME-Version: 1.0 10, 44 -- Content-Type: text/plain; 10, 44 -- charset="us-ascii" 10, 44 -- Content-Transfer-Encoding: 7bit 10, 44 -- X-Mailer: Microsoft Outlook 14.0 10, 44 -- Thread-Index: AQJwEA55ksWYCeFAf44NBX/BI5A8nJl55O1Q 10, 44 -- Content-Language: en-us 10, 44 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 10, 44 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com 10, 44 -- X-AntiAbuse: Original Domain - microscopy.com 10, 44 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 10, 44 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com 10, 44 -- X-BWhitelist: no 10, 44 -- X-Source-IP: 146.115.5.114 10, 44 -- X-Source: 10, 44 -- X-Source-Args: 10, 44 -- X-Source-Dir: 10, 44 -- X-Source-Sender: (LESZSG) [146.115.5.114]:63647 10, 44 -- X-Source-Auth: les-at-zsgenetics.com 10, 44 -- X-Email-Count: 2 10, 44 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20= ==============================End of - Headers==============================
I've used the GIMP for this application with a lot of success. Something else to look at would be this: http://research.microsoft.com/en-us/um/redmond/groups/ivm/ICE/
-Jacob
On 14-02-18 02:29 PM, peter.eschbach-at-comcast.net wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images. } } Pete Eschbach } Oregon State University } } Sent from my iPhone } } ==============================Original Headers============================== } 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 } 3, 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) } 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 } 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 } 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) } 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast } 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 } 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) } 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast } 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 } 3, 32 -- Subject: Montage software? } 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} } 3, 32 -- Content-Type: text/plain; } 3, 32 -- charset=us-ascii } 3, 32 -- X-Mailer: iPhone Mail (11B554a) } 3, 32 -- Message-Id: {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} } 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 } 3, 32 -- To: Microscopy-at-microscopy.com } 3, 32 -- Mime-Version: 1.0 (1.0) } 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; } 3, 32 -- s=q20121106; t=1392761924; } 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; } 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: } 3, 32 -- Mime-Version; } 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 } 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 } 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y } 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m } 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC } 3, 32 -- vXeoTIY3Fw3YA== } 3, 32 -- Content-Transfer-Encoding: 8bit } 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IMIi4s005720 } ==============================End of - Headers============================== } }
==============================Original Headers============================== 3, 28 -- From jkabel-at-mail.ubc.ca Tue Feb 18 18:33:21 2014 3, 28 -- Received: from vmaprod4.mail-relay.ubc.ca (vmaprod4.mail-relay.ubc.ca [142.103.117.138]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1J0XLbt031713 3, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 18:33:21 -0600 3, 28 -- Received: from vmaprod4.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 3, 28 -- by localhost (Email Security Appliance) with SMTP id D179326B941_303FBD0B 3, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 00:33:20 +0000 (GMT) 3, 28 -- Received: from mx2.mail-relay.ubc.ca (lb_mx-out.mail-relay.ubc.ca [10.92.8.24]) 3, 28 -- by vmaprod4.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTP id 9981826BA7D_303FBD0F 3, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 00:33:20 +0000 (GMT) 3, 28 -- Received: from smtp.mail.ubc.ca (s-itsv-hub04p.ead.ubc.ca [137.82.151.86]) 3, 28 -- by mx2.mail-relay.ubc.ca (Postfix) with ESMTP id 9153615F66A 3, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:33:20 -0800 (PST) 3, 28 -- Received: from [137.82.16.57] (137.82.16.57) by smtp.mail.ubc.ca 3, 28 -- (137.82.151.86) with Microsoft SMTP Server id 14.3.174.1; Tue, 18 Feb 2014 3, 28 -- 16:33:20 -0800 3, 28 -- Message-ID: {5303FBD0.2000509-at-mail.ubc.ca} 3, 28 -- Date: Tue, 18 Feb 2014 16:33:20 -0800 3, 28 -- From: Jacob Kabel {jkabel-at-mail.ubc.ca} 3, 28 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 3, 28 -- MIME-Version: 1.0 3, 28 -- To: {Microscopy-at-microscopy.com} 3, 28 -- Subject: Re: [Microscopy] Montage software? 3, 28 -- References: {201402182229.s1IMTlmO021444-at-ns.microscopy.com} 3, 28 -- In-Reply-To: {201402182229.s1IMTlmO021444-at-ns.microscopy.com} 3, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 28 -- Content-Transfer-Encoding: 7bit 3, 28 -- X-Originating-IP: [137.82.16.57] ==============================End of - Headers==============================
I have used the commercial program Corel Photopaint (part of the Coreldraw suite of programs) extensively over the years for TEM and other montaging. It is manual montaging, but it handles any file size (I have done up to montage around 300 GB), and it has a feature where the selected image is subtracted from what is under it, meaning you can quickly see which parts of the image are aligned because they go completely black.
The programs others have mentioned are also good options.
Kind regards Duane Harland AgResearch New Zealand
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Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images.
Pete Eschbach Oregon State University
Sent from my iPhone
==============================Original Headers============================== 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 3, 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 3, 32 -- Subject: Montage software? 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 3, 32 -- Content-Type: text/plain; 3, 32 -- charset=us-ascii 3, 32 -- X-Mailer: iPhone Mail (11B554a) 3, 32 -- Message-Id: {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 3, 32 -- To: Microscopy-at-microscopy.com 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 3, 32 -- s=q20121106; t=1392761924; 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: 3, 32 -- Mime-Version; 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC 3, 32 -- vXeoTIY3Fw3YA== 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IMIi4s005720 ==============================End of - Headers==============================
"Metallographic etchnig" by Gűnter Petzow edited by ASM 1999, black white photo lead structure was published in the practicall metalography many years ago (1970 ?)
best regards
best regards
Chris Hűbner
Cracow University of Technology Faculty of Mechanical Engineering
----- Original Message ----- X-from: {oshel1pe-at-cmich.edu} To: {hubner-at-mech.pk.edu.pl} Sent: Monday, February 17, 2014 2:50 PM
Dear Microscopists,
maybe someone can give me a hint on how to proceed now. We just encountered a problem with our Tecnai Osiris in STEM mode: We reduced the acceleration voltage from 200kV to 80kV to deal with a little bit sensitive samples. In TEM Mode we didn´t had any problems, but in STEM we see a distortion. I uploaded a picture* of the problem. We suspect some magnetic field could be responsible for this behavior.
What do you think? And what could we do to find the reason?
I start with double sticky conductive tape on a stub. I spread the powder out on a glass slide and blow the powder off the slide and onto the stub. Just a puff, a little breath, a mouse fart. A small mouse.
You can position the stub below or across from your slide. Too much powder on stub? Blow it off, use a bigger mouse this time.
What you want is a single layer of crystals/particles, separated as much as possible from each other. The stub will look sample deficient to the eye, check it with a stereomicroscope and I suspect you'll find plenty.
I coat these samples with Au or Pt, twice. One coating straight on and then I lay the SEM mount in the chamber at an angle to get the "underside" of the particles. Works great. I've been running organic accelerators for rubber this way for years and have always been happy with results.
Good Luck!
Frank
-----Original Message----- X-from: mike-at-materialanalyzers.com [mailto:mike-at-materialanalyzers.com] Sent: Tuesday, February 18, 2014 4:03 PM To: Frank Karl
I would appreciate hearing suggestions for a good mounting technique for imaging activated carbon and synthetic diamond particles (1-200 micron). When using standard conductive double sided adhesive carbon tabs on stubs, it is obviously quite challenging to get adequate contrast between the particles and the background (carbon tab).
Likewise, in doing particle size analysis it helps to not have touching particles and clumps. Any techniques that have been found to be successful would be interesting to hear.
Thank You!!
Mike Toalson Western Analytical Solutions, LLC o * 208.899.4425 m * 208.340.3895 w * www.materialanalyzers.com e * mike-at-materialanalyzers.com
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==============================Original Headers============================== 27, 29 -- From frank_karl-at-ardl.com Wed Feb 19 09:26:37 2014 27, 29 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 27, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JFQaP8023143 27, 29 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 09:26:36 -0600 27, 29 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 27, 29 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Wed, 19 27, 29 -- Feb 2014 10:26:35 -0500 27, 29 -- From: Frank Karl {frank_karl-at-ardl.com} 27, 29 -- To: "'mike-at-materialanalyzers.com'" {mike-at-materialanalyzers.com} 27, 29 -- CC: "Microscopy Listserver (microscopy-at-microscopy.com)" 27, 29 -- {microscopy-at-microscopy.com} 27, 29 -- Date: Wed, 19 Feb 2014 10:26:34 -0500 27, 29 -- Subject: RE: [Microscopy] SEM - Suggested mounting method for Carbon 27, 29 -- particles 27, 29 -- Thread-Topic: [Microscopy] SEM - Suggested mounting method for Carbon 27, 29 -- particles 27, 29 -- Thread-Index: Ac8s7OEK3Dkn4B9+TbWCOUe2HKie+gAmFesg 27, 29 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BD2D1B130-at-exchange2k7.ad.ardl.com} 27, 29 -- References: {201402182102.s1IL2aHo031064-at-ns.microscopy.com} 27, 29 -- In-Reply-To: {201402182102.s1IL2aHo031064-at-ns.microscopy.com} 27, 29 -- Accept-Language: en-US 27, 29 -- Content-Language: en-US 27, 29 -- X-MS-Has-Attach: 27, 29 -- X-MS-TNEF-Correlator: 27, 29 -- acceptlanguage: en-US 27, 29 -- Content-Type: text/plain; charset="iso-8859-1" 27, 29 -- MIME-Version: 1.0 27, 29 -- Content-Transfer-Encoding: 8bit 27, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1JFQaP8023143 ==============================End of - Headers==============================
3. For coloring lead phases try the Copper Alloy etch set out by:
Beraha: Color Metallography. ASM 1977.
4. Coloring can be an advantage if it adds to the contrast desired to detect or demonstrate a particular phase. This will depend on the material at hand.
5. M Raja, please report back how it turns out.
Tony
…………………………………………………………………………………………………………… Andrew Anthony “Tony” Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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-----Original Message----- X-from: hubner-at-mech.pk.edu.pl [mailto:hubner-at-mech.pk.edu.pl] Sent: Wednesday, February 19, 2014 7:19 AM To: ph2-at-sprynet.com
Hello,
lead etching you found in the book:
"Metallographic etchnig" by Gűnter Petzow edited by ASM 1999, black white photo lead structure was published in the practicall metalography many years ago (1970 ?)
best regards
best regards
Chris Hűbner
Cracow University of Technology Faculty of Mechanical Engineering
----- Original Message ----- X-from: {oshel1pe-at-cmich.edu} To: {hubner-at-mech.pk.edu.pl} Sent: Monday, February 17, 2014 2:50 PM
Hi
Beckert - Klemm "Handbuch der metallographischen Ätzverfahren" VEB Deutsch. Verlag f. Grundstoffindustrie - 1962
Sorry, it's in german, but very usefull. Always on my shelf !
There are 10 pages with different solutions vor lead and lead alloys, with 2 BW pictures and an (old now) bibliography.
Hope it helps
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
} Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images.
a number of options to stitch existing images has been discussed already - if you are interested in software that drives automated acquisition to build montages, have a look at SerialEM (actually a tomography software):
http://bio3d.colorado.edu/SerialEM/index.html
It works on the Titan platform and can also do the stitching.
Best,
Guenter
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
==============================Original Headers============================== 8, 19 -- From lists-at-nexperion.net Wed Feb 19 11:33:43 2014 8, 19 -- Received: from sh-503.premium.hosttech.eu (sh-503.premium.hosttech.eu [46.4.82.76]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JHXhvh017355 8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 11:33:43 -0600 8, 19 -- X-No-Relay: not in my network 8, 19 -- Received: from aventine (chello080108026214.8.11.vie.surfer.at [80.108.26.214]) 8, 19 -- by sh-503.premium.hosttech.eu (Postfix) with ESMTPSA id CB6BA11F502E 8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 18:33:41 +0100 (CET) 8, 19 -- Date: Wed, 19 Feb 2014 18:33:39 +0100 8, 19 -- From: Guenter Resch {lists-at-nexperion.net} 8, 19 -- To: Microscopy-at-microscopy.com 8, 19 -- Subject: Re: [Microscopy] Montage software? 8, 19 -- Message-ID: {20140219183339.1b468998-at-aventine} 8, 19 -- In-Reply-To: {201402182230.s1IMUhOr023549-at-ns.microscopy.com} 8, 19 -- References: {201402182230.s1IMUhOr023549-at-ns.microscopy.com} 8, 19 -- X-Mailer: Claws Mail 3.8.1 (GTK+ 2.24.20; x86_64-pc-linux-gnu) 8, 19 -- Mime-Version: 1.0 8, 19 -- Content-Type: text/plain; charset=US-ASCII 8, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Montage building software you use will be dependent not so much on the microscope but how the images are named and in what order they are collected. If you are using a FEI Eagle camera, you will need to convert the proprietary image format to .tif or .jpg then proceed. GIMP does this well. We use an AMT camera and the images are automatically named according to their x-y position in the montage, for example 0006R1C6 refers to an image in the first row and the sixth column. How the stage moves from one image to the next during collection is essential information. We have used AdobePhotoshop for image montages, but it is slow compared to FIJI and may not complete large montages. For montages up to 19x19 images, I would suggest using FIJI. For image sets of this size you will also want a 64 bit operating system with exceptional graphics capabilities. Download Fiji (http://fiji.sc/wiki/index.php/Downloads). Fiji is a distribution of ImageJ together with Java, Java 3D and several plugins organized to assist research in life sciences, targeting image registration, stitching, segmentation, feature extraction and 3D visualization, among others. Using FIJI you can direct the program to the folder in which the images are stored and also define the naming nomenclature so that the montage software will find each successive image. There is quite a bit of flexibility built into FIJI as to how the program finds the images; we have had the most success with the position defined by the file name. Once you download FIJI, bring up the screen where images in this format can be loaded (FIJI/Stitching/Deprecated/Stitch grid of images). I would be happy to send a word document with specific instructions with screen shots on how to work with images in this format to anyone who might find it useful.
Cheers,
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3101 SW Sam Jackson Park Road Portland, Oregon 97239 Office: 503-221-3434 Mobile: 503-819-3600
} Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images. } } Pete Eschbach } Oregon State University } } Sent from my iPhone } } ==============================Original } Headers============================== } 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 3, } 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) } 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 } 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 } 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) } 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast } 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 } 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) } 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast } 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 } 3, 32 -- Subject: Montage software? } 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 3, 32 -- } Content-Type: text/plain; } 3, 32 -- charset=us-ascii } 3, 32 -- X-Mailer: iPhone Mail (11B554a) 3, 32 -- Message-Id: } {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} } 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 3, 32 -- To: } Microscopy-at-microscopy.com 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- } DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; } 3, 32 -- s=q20121106; t=1392761924; } 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; } 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: } 3, 32 -- Mime-Version; } 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 } 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 } 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y } 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m } 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC } 3, 32 -- vXeoTIY3Fw3YA== } 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s1IMIi4s005720 ==============================End } of - Headers============================== } }
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==============================Original Headers============================== 6, 36 -- From DRK-at-shcc.org Wed Feb 19 11:56:28 2014 6, 36 -- Received: from mail1.bemta8.messagelabs.com (mail1.bemta8.messagelabs.com [216.82.243.203]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JHuRA6004094 6, 36 -- for {Microscopy-at-Microscopy.Com} ; Wed, 19 Feb 2014 11:56:28 -0600 6, 36 -- Received: from [216.82.242.115:2097] by server-11.bemta-8.messagelabs.com id 16/E0-30795-B40F4035; Wed, 19 Feb 2014 17:56:27 +0000 6, 36 -- X-Env-Sender: DRK-at-shcc.org 6, 36 -- X-Msg-Ref: server-13.tower-132.messagelabs.com!1392832494!12679344!105 6, 36 -- X-Originating-IP: [216.166.12.178] 6, 36 -- X-StarScan-Received: 6, 36 -- X-StarScan-Version: 6.9.16; banners=-,-,- 6, 36 -- X-VirusChecked: Checked 6, 36 -- Received: (qmail 2235 invoked from network); 19 Feb 2014 17:56:27 -0000 6, 36 -- Received: from out001.collaborationhost.net (HELO out001.collaborationhost.net) (216.166.12.178) 6, 36 -- by server-13.tower-132.messagelabs.com with RC4-SHA encrypted SMTP; 19 Feb 2014 17:56:27 -0000 6, 36 -- Received: from AUSP01VMBX30.collaborationhost.net ([10.2.12.44]) by 6, 36 -- AUSP01MHUB04.collaborationhost.net ([10.2.0.189]) with mapi; Wed, 19 Feb 2014 6, 36 -- 11:57:15 -0600 6, 36 -- From: Doug Keene {DRK-at-shcc.org} 6, 36 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 6, 36 -- Date: Wed, 19 Feb 2014 11:56:21 -0600 6, 36 -- Subject: RE: [Microscopy] Re: Montage software? 6, 36 -- Thread-Topic: [Microscopy] Re: Montage software? 6, 36 -- Thread-Index: Ac8tC2iZmfHZnAiGS2mQq5ze4h96RAAjKW7g 6, 36 -- Message-ID: {1DDBEFFEDA3EED4B8817469E593164AA6BC94881DA-at-AUSP01VMBX30.collaborationhost.net} 6, 36 -- References: {201402190041.s1J0f6sk011088-at-ns.microscopy.com} 6, 36 -- In-Reply-To: {201402190041.s1J0f6sk011088-at-ns.microscopy.com} 6, 36 -- Accept-Language: en-US 6, 36 -- Content-Language: en-US 6, 36 -- X-MS-Has-Attach: 6, 36 -- X-MS-TNEF-Correlator: 6, 36 -- x-vipre-scanned: 25769B1B00680C25769C68 6, 36 -- acceptlanguage: en-US 6, 36 -- Content-Type: text/plain; charset="us-ascii" 6, 36 -- MIME-Version: 1.0 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1JHuRA6004094 ==============================End of - Headers==============================
There are many montaging programs. I can suggest PTGUI which some of our SEM users have used for montages with hundreds of images. It is not picky on the filenames, filetypes, and/or file order.
PTGUI stands for Panorama Tools (Graphic User Interface).
regards,
- Jim
==============================Original Headers============================== 5, 26 -- From jquinn11733-at-gmail.com Wed Feb 19 14:46:21 2014 5, 26 -- Received: from mail-la0-f51.google.com (mail-la0-f51.google.com [209.85.215.51]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JKkKcq026122 5, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 14:46:21 -0600 5, 26 -- Received: by mail-la0-f51.google.com with SMTP id c6so688718lan.24 5, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 12:46:20 -0800 (PST) 5, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 26 -- d=gmail.com; s=20120113; 5, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 5, 26 -- bh=nY7stHr3bJct1VSuuYydCgMXvAzlDnrv1yJfpTVQ7As=; 5, 26 -- b=Ej4EM/ezeoHDTdgxKuh/tldikL1G8PkboGaY0jb1PtDJNjse9MFC6RtQ/18V2eKUm8 5, 26 -- ERgimw7tN0j7BduVU3WF77OMZpb5IEK90TsUfBazdURebUeWqOUvQlecwCxPkfeyl4of 5, 26 -- iUfZPjfaXNzig/cI4u5pzAlZJJxxQUn5HhyxVdD03ZwEQMBFxfFD/yxfo5zO3BbANSlO 5, 26 -- cnGpDY8B5+Em8P02zOSCeK1z6vjusQsjIpgfEX/Glvb3J8rHj/3UFSsj3+Vyf3aOxkPy 5, 26 -- j+9PWupEmY/xYbYYCg9rzg46TchTQF20ikwOUK/EpgSAqaqkewZcF1I9wc4dcZRIOE6e 5, 26 -- 3Tdw== 5, 26 -- MIME-Version: 1.0 5, 26 -- X-Received: by 10.112.56.237 with SMTP id d13mr26179360lbq.2.1392842780112; 5, 26 -- Wed, 19 Feb 2014 12:46:20 -0800 (PST) 5, 26 -- Received: by 10.112.218.3 with HTTP; Wed, 19 Feb 2014 12:46:20 -0800 (PST) 5, 26 -- Date: Wed, 19 Feb 2014 15:46:20 -0500 5, 26 -- Message-ID: {CAO_SM8KtqaPa4Aht6vAwQNiqSOUmd+Q6ke8nKZorTCZ=-ezbdw-at-mail.gmail.com} 5, 26 -- Subject: montage with PTGUI 5, 26 -- From: Jim Quinn {jquinn11733-at-gmail.com} 5, 26 -- To: Microscopy-at-microscopy.com 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
I did not send any of you a message in the last two days. Please do not open if you did receive one for I do not know where the message originated. -- Pat Patricia Stranen Connelly NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491
==============================Original Headers============================== 3, 58 -- From connellyps-at-nhlbi.nih.gov Wed Feb 19 16:31:06 2014 3, 58 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 3, 58 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JMV6wx014141 3, 58 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 16:31:06 -0600 3, 58 -- X-IronPortListener: Outbound_SMTP 3, 58 -- X-IronPort-Anti-Spam-Filtered: true 3, 58 -- X-IronPort-Anti-Spam-Result: AgEFAOYvBVOcKEel/2dsb2JhbABWAw6CeIEPvQGEHRZ0giwSFxFRAQITKS8TJwQBNIdjol6rcReOAhEBaIQnBI5kiUyGb4s1gm4/gXE5 3, 58 -- Received: from unknown (HELO CASHTV01.nih.gov) ([156.40.71.165]) 3, 58 -- by nihxway3out.hub.nih.gov with ESMTP/TLS/AES128-SHA; 19 Feb 2014 17:31:05 -0500 3, 58 -- Received: from MLBXV14.nih.gov ([169.254.4.167]) by CASHTV01.nih.gov 3, 58 -- ([156.40.71.165]) with mapi id 14.03.0174.001; Wed, 19 Feb 2014 17:31:01 3, 58 -- -0500 3, 58 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 3, 58 -- To: ChuckCharles A Garber {cgarber-at-2spi.com} , 3, 58 -- DanDan Connelly 3, 58 -- {dansconnelly-at-hotmail.com} , 3, 58 -- "Sherman, Debra" {dsherman-at-purdue.edu} , 3, 58 -- "DeeDee 3, 58 -- Breger" {deebre-at-coe.drexel.edu} , 3, 58 -- DottyDorothy Sorenson {dsoren-at-umich.edu} , 3, 58 -- GregGreg Guild {gguild-at-sas.upenn.edu} , 3, 58 -- JoelJoel Sheffield {jbs-at-temple.edu} , 3, 58 -- LouiseLouise Harner {louise_harner-at-albint.com} , 3, 58 -- mePat Connelly 3, 58 -- {stranen_connelly-at-yahoo.com} , 3, 58 -- MSA {microscopy-at-microscopy.com} , 3, 58 -- "NoelNoel 3, 58 -- Stranen" {noeltimbo-at-aol.com} , 3, 58 -- SheilaSheila Connelly 3, 58 -- {sheila.connelly-at-avtxinc.com} , 3, 58 -- suekiShoji Ueki {sueki-at-wharton.upenn.edu} , 3, 58 -- TatyanaTatyana Svitkina {svitkina-at-sas.upenn.edu} , 3, 58 -- "Connelly, Patricia 3, 58 -- (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} , 3, 58 -- Bernadette Lawrence 3, 58 -- {bmcl80-at-comcast.net} , 3, 58 -- borders {borders-at-e.bordersstores.com} , 3, 58 -- Dave Heminghaus 3, 58 -- {dheminghaus-at-semtechsolutions.com} , 3, 58 -- David L Jones {dljones-at-bestweb.net} , David Roos {droos-at-sas.upenn.edu} 3, 58 -- Subject: URGENT Do not open earlier email from me 3, 58 -- Thread-Topic: URGENT Do not open earlier email from me 3, 58 -- Thread-Index: AQHPLcJGL9dWjyyUyEePDnY0awitNw== 3, 58 -- Importance: high 3, 58 -- X-Priority: 1 3, 58 -- Date: Wed, 19 Feb 2014 22:31:04 +0000 3, 58 -- Message-ID: {CF2A9AD5.17C2%connellyps-at-mail.nih.gov} 3, 58 -- Accept-Language: en-US 3, 58 -- Content-Language: en-US 3, 58 -- X-MS-Has-Attach: 3, 58 -- X-MS-TNEF-Correlator: 3, 58 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 3, 58 -- x-originating-ip: [156.40.71.188] 3, 58 -- Content-Type: text/plain; charset="us-ascii" 3, 58 -- Content-ID: {36A16DC804ABD146BA1BBD33A896DF44-at-mail.nih.gov} 3, 58 -- MIME-Version: 1.0 3, 58 -- Content-Transfer-Encoding: 8bit 3, 58 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1JMV6wx014141 ==============================End of - Headers==============================
My mail has been hacked or whatever you you call it. I am attempting to find which email was broken into but this was the fastest way to get to a lot of you. I have not sent any messages to the Listerv earlier today.
-- Pat Patricia Stranen Connelly NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491
==============================Original Headers============================== 5, 30 -- From connellyps-at-nhlbi.nih.gov Wed Feb 19 16:42:44 2014 5, 30 -- Received: from nihxway4out.hub.nih.gov (nihxway4out.hub.nih.gov [128.231.90.112]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JMgh2C027234 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 16:42:43 -0600 5, 30 -- X-IronPortListener: Outbound_SMTP 5, 30 -- X-IronPort-Anti-Spam-Filtered: true 5, 30 -- X-IronPort-Anti-Spam-Result: AgEFAMcyBVOcKEeo/2dsb2JhbABWA4MGgQ+9AYQdFnSCLBIoUQEVKS8TJwQ1h2OdaoR2q3EXjgIQAgFnhCcEjmSJTIZvizWDLYIq 5, 30 -- Received: from unknown (HELO CASHTV04.nih.gov) ([156.40.71.168]) 5, 30 -- by nihxway4out.hub.nih.gov with ESMTP/TLS/AES128-SHA; 19 Feb 2014 17:42:43 -0500 5, 30 -- Received: from MLBXV14.nih.gov ([169.254.4.167]) by CASHTV04.nih.gov 5, 30 -- ([156.40.71.168]) with mapi id 14.03.0174.001; Wed, 19 Feb 2014 17:42:43 5, 30 -- -0500 5, 30 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 5, 30 -- To: MSA {microscopy-at-microscopy.com} 5, 30 -- Subject: URGENT do NOT open any mail from Pat Connelly today 5, 30 -- Thread-Topic: URGENT do NOT open any mail from Pat Connelly today 5, 30 -- Thread-Index: AQHPLcPm1AJBqxCyf0WpHAg2ovBQWA== 5, 30 -- Date: Wed, 19 Feb 2014 22:42:42 +0000 5, 30 -- Message-ID: {CF2A9D91.17E5%connellyps-at-mail.nih.gov} 5, 30 -- Accept-Language: en-US 5, 30 -- Content-Language: en-US 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 5, 30 -- x-originating-ip: [156.40.71.192] 5, 30 -- Content-Type: text/plain; charset="us-ascii" 5, 30 -- Content-ID: {AA896E870F9CF3428DB766ED7EBFD453-at-mail.nih.gov} 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1JMgh2C027234 ==============================End of - Headers==============================
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Email: alpinto-at-fm.ul.pt Name: Andreia Pinto
Organization: Instituto de Medicina Molecular
Title-Subject: [Filtered] JEOL JEM-100CX II diaphragms
Message: We have here at the Institute an old Jeol JEM-100CX II that was disconnected for many years, now it is finally working again, but, it needs new diaphragms, so, we are searching for good options in acquiring/buying diaphragms for this brand/model, can you help me?? We really can't spend a lot of money, so, we are also prone to accept used diaphragms, if they are in good shape.
Thank you very much!!
Andreia L. Pinto Comparative Pathology Laboratory Instituto Medicina Molecular Lisbon - Portugal
e-mail: alpinto-at-fm.ul.pt
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 19 17:08:09 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JN89gY027659 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 17:08:09 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 3E6C26C1330 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 17:08:09 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id bsGzJl+6YdlL for {microscopy-at-microscopy.com} ; 19, 28 -- Wed, 19 Feb 2014 17:08:02 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 59F776C1323 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 17:08:02 -0600 (CST) 19, 28 -- Message-ID: {53053952.7060500-at-microscopy.com} 19, 28 -- Date: Wed, 19 Feb 2014 17:08:02 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:JEOL JEM-100CX II diaphragms 19, 28 -- References: {201402191359.s1JDx5tI002583-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201402191359.s1JDx5tI002583-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201402191359.s1JDx5tI002583-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Max, You're probably seeing the effect of a stray AC field on your scan. A paper by Muller et al. from Ultramicroscopy describes ways to design a room to minimize this kind of distortion: http://www.sciencedirect.com/science/article/pii/S0304399106001033
Do you notice the distortion change when you change your scan rate? Right now, it appears that the period of the field's oscillation is equal to the time necessary to complete a couple of scan lines. If you can find a scan rate at which the distortion period is equal to the time necessary to complete an integer number of scan lines, you should be able to calculate the frequency of the distortion (I bet it's 60 Hz!) which may help you locate the source of the field.
Tyler
On 2014-02-19 6:50, raven2020-at-gmx.de wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Microscopists, } } maybe someone can give me a hint on how to proceed now. } We just encountered a problem with our Tecnai Osiris in } STEM mode: } We reduced the acceleration voltage from 200kV to 80kV } to deal with a little bit sensitive samples. In TEM Mode } we didn´t had any problems, but in STEM we see a distortion. } I uploaded a picture* of the problem. } We suspect some magnetic field could be responsible for this } behavior. } } What do you think? And what could we do to find the reason? } } Thank you in advance! } } best regards, } } Max } } } * https://www.dropbox.com/s/1ued2rl94y81rfo/19-02-2014-80kV_HAADF.jpg } } ==============================Original } Headers============================== } 8, 21 -- From raven2020-at-gmx.de Wed Feb 19 08:36:53 2014 } 8, 21 -- Received: from mout.gmx.net (mout.gmx.net [212.227.15.18]) } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s1JEaqrx003083 } 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 08:36:53 } -0600 } 8, 21 -- Received: from [172.16.44.168] ([192.124.253.68]) by } mail.gmx.com (mrgmx001) } 8, 21 -- with ESMTPSA (Nemesis) id 0MQiVh-1Whspq3RwD-00U3TL for } 8, 21 -- {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 15:36:51 +0100 } 8, 21 -- Message-ID: {5304C181.1080301-at-gmx.de} } 8, 21 -- Date: Wed, 19 Feb 2014 15:36:49 +0100 } 8, 21 -- From: Max Klingsporn {raven2020-at-gmx.de} } 8, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:24.0) } Gecko/20100101 Thunderbird/24.3.0 } 8, 21 -- MIME-Version: 1.0 } 8, 21 -- To: microscopy-at-microscopy.com } 8, 21 -- Subject: Problem with STEM at 80kV } 8, 21 -- X-Enigmail-Version: 1.6 } 8, 21 -- Content-Type: text/plain; charset=UTF-8 } 8, 21 -- Content-Transfer-Encoding: 8bit } 8, 21 -- X-Provags-ID: } V03:K0:Jd85cpcfmJhK89zoT/+VwZUNCcLvN10eO4UDzOBl0xw5Xk6fWbR } 8, 21 -- } PMGPjbOUv7YqJ6XEmvSm8ErxIMqdNRahX8NUt7t8IjN2f77twuLS6zeYUp2QR+1z0pgKwUi } 8, 21 -- } HzXZk+N70BRYGOw7z1hdGfvnQw3YipD6OVw01YMu/SUeRv4q6PkTfrFfwRYOFyir5SP2y/y } 8, 21 -- SdCfgF68P5Em3S6Pj7KfA== } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 31 -- From trh-at-uoregon.edu Wed Feb 19 19:37:51 2014 4, 31 -- Received: from smtp.uoregon.edu (oh-mserv2.uoregon.edu [128.223.142.145]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1K1bpE9019685 4, 31 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 19:37:51 -0600 4, 31 -- Received: from webmail.uoregon.edu (webmail1.uoregon.edu [128.223.143.235] (may be forged)) 4, 31 -- (authenticated bits=0) 4, 31 -- by smtp.uoregon.edu (8.14.7/8.14.5) with ESMTP id s1K1bjqk019259 4, 31 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 4, 31 -- Wed, 19 Feb 2014 17:37:45 -0800 4, 31 -- Received: from [2001:468:d01:14:223:aeff:fe71:fe9d] 4, 31 -- by webmail.uoregon.edu 4, 31 -- with HTTP (HTTP/1.1 POST); Wed, 19 Feb 2014 17:37:44 -0800 4, 31 -- MIME-Version: 1.0 4, 31 -- Content-Type: text/plain; charset=UTF-8; 4, 31 -- format=flowed 4, 31 -- Content-Transfer-Encoding: 8bit 4, 31 -- Date: Wed, 19 Feb 2014 17:37:44 -0800 4, 31 -- From: Tyler R Harvey {trh-at-uoregon.edu} 4, 31 -- To: raven2020-at-gmx.de 4, 31 -- Cc: microscopy-at-microscopy.com 4, 31 -- Subject: Re: [Microscopy] Problem with STEM at 80kV 4, 31 -- In-Reply-To: {201402191450.s1JEoOAI021491-at-ns.microscopy.com} 4, 31 -- References: {201402191450.s1JEoOAI021491-at-ns.microscopy.com} 4, 31 -- Message-ID: {94c5a17e18192630ad49aeb3e0d52338-at-uoregon.edu} 4, 31 -- X-Sender: trh-at-uoregon.edu 4, 31 -- User-Agent: Roundcube Webmail/0.9.5 4, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.11.87,1.0.14,0.0.0000 4, 31 -- definitions=2014-02-19_07:2014-02-18,2014-02-19,1970-01-01 signatures=0 4, 31 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=4 phishscore=0 4, 31 -- adultscore=0 bulkscore=0 classifier=spam adjust=-40 reason=mlx scancount=1 4, 31 -- engine=7.0.1-1305240000 definitions=main-1402190177 ==============================End of - Headers==============================
Good evening everyone, I am have some problem with my formvar grids. till 6 months ago they were all good but now all (i even checked new one from sealed box) of them are extremely fragile under beam (they tear apart even while going from low mag to high mag mode). Not only that, it seems they are developing some sort of static charge as beam shifts can be seen inside microscope when i insert those grids. here is attached pics of how a beam edge (a shadow of objective aperture) is behaving near a hole (made during changing mode) in formvar substrate. Any ideas what am doing wrong?
https://sites.google.com/site/auxilliarylinks/
Regards Amit Gupta Research Scholar Dept. of Inorganic and Physical Chemistry, IISc
==============================Original Headers============================== 3, 26 -- From amit.welcomes.u-at-gmail.com Wed Feb 19 23:51:11 2014 3, 26 -- Received: from mail-wg0-f43.google.com (mail-wg0-f43.google.com [74.125.82.43]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1K5pBpx009868 3, 26 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 23:51:11 -0600 3, 26 -- Received: by mail-wg0-f43.google.com with SMTP id a1so1104068wgh.22 3, 26 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 21:51:10 -0800 (PST) 3, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=20120113; 3, 26 -- h=mime-version:from:date:message-id:subject:to:content-type; 3, 26 -- bh=V4MuM70me0UbYmQ4M3YN3oNvI4WgIWNjq2t6dr/q/Mk=; 3, 26 -- b=L8whLVOdL5W+peLBxu5ePqqBQdi8vpDFJb3nvqqJsEQPz9XgVGVi1XVnF5rgOwa4KJ 3, 26 -- 5Ux++dOK9YIWgShojXU9akCxi/K9JBiRj/n7gKJY6P+vBzGrm2LcinxnDmjTU4gVPs5v 3, 26 -- Je1+vYx3aIfDBPEpyhzEyNfEoIGnktCDOM8LgrEhkoGz/+xrEVYG0T2byE37yP62XzIv 3, 26 -- vrFQBABWnsRq2UCWmVOg/3eASwe5exoF9kpcbbVD3ko2+QuEqbRIN9tGL1ad8XABqIgv 3, 26 -- ynjjpZirhu6nPPOhQenu60zv0DRLs9aCkA1ghLsCWiOrBgYgzLaPR+qPX4OEPq/B6vVw 3, 26 -- vGsQ== 3, 26 -- X-Received: by 10.194.63.228 with SMTP id j4mr32514004wjs.34.1392875470300; 3, 26 -- Wed, 19 Feb 2014 21:51:10 -0800 (PST) 3, 26 -- MIME-Version: 1.0 3, 26 -- Received: by 10.194.179.202 with HTTP; Wed, 19 Feb 2014 21:50:50 -0800 (PST) 3, 26 -- From: Amit Gupta {amit.welcomes.u-at-gmail.com} 3, 26 -- Date: Thu, 20 Feb 2014 11:20:50 +0530 3, 26 -- Message-ID: {CA+bx8_zpVtgSFU9SYmQMQnzOjJohAs-it0y5foXUi5NJnN1hKw-at-mail.gmail.com} 3, 26 -- Subject: Formvar grids having static? 3, 26 -- To: microscopy {microscopy-at-microscopy.com} 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
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Email: kristian.molhave-at-nanotech.dtu.dk Name: Kristian Molhave
Organization: Technical University of Denmark, Dept. of Micro and Nanotechnology
Title-Subject: [Filtered] Two PhD positions open in electron microscopy of processes in liquids
Message: The Department of Micro- and Nanotechnology invites applications for two 3-year PhD Scholarships on studying nanoscale processes in liquids with electron microscopy:
PhD scholarship in Electron microscopy of electrochemical processes in liquids
for more information see http://www.nanotech.dtu.dk/english/About-NTCH/Jobs/job?id=0673b787-8118-4951-af3b-2e0925f70d70
and
PhD scholarship in Nanoscale Imaging of the Aqueous Processes of Precipitation, Dispersion, and Imbibition
for more information see http://www.nanotech.dtu.dk/english/About-NTCH/Jobs/job?id=e41ab81b-5adf-4a86-bc5e-ef10cc5610a9
The Deadline for applications is 27 and 28th february.
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RJ Perz-Edwards- I also suspected the same problem hence cleaned the holder properly, though did not check the top part. Well most of grids are little vulnerable during that transition but now they are almost sure to break apart. also if u do stem you can see them expanding and breaking. It reders them almost useless. shashi singh- no we are buying it. Glow discharge is not available at the moment to try but i did try grounding them while making sample... did not help. Will try ethanol shortly. Ross- Although manufacturer is same but they are copper only, we can see it in EDS. Besides they were working perfectly before, how come all of sudden?
Regards Amit Gupta Research Scholar Prof. S.Vasudevan's lab Dept. of Inorganic and Physical Chemistry, IISc
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Dear Listservers, I am planning an HPF hybrid (pre-light chemical fixation) technique on attached cells, for HM-20 post embed IEM. My plan is to lightly fix the cells for 1 hr (4% PF 0.5% Ga 0.1M HEPES with 5mM MgCl2), rinse in 0.1M HEPES 3.5% sucrose with 5mM MgCl2 (243 mOsmols), then scrape and pellet the cells. I will resuspend the pellet in 2% agarose (in the cells media minus serum) just before HPF. My question is has anyone used HEPES as an IEM buffer, and at what concentration (molarity)? I have read that a slightly hypotonic solution is better for IEM, I believe the cultured cells are isotonic at 300 mOsmols. Also for IEM, is there really a difference between 4% PF and 2% PF? Any suggestions are welcome.
sincerely, Michael Delannoy
==============================Original Headers============================== 2, 32 -- From prvs=1211802d7=delannoy-at-jhmi.edu Thu Feb 20 12:40:37 2014 2, 32 -- Received: from IPEX5.johnshopkins.edu (ipex5.johnshopkins.edu [128.220.161.72]) 2, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1KIeb4e013212; 2, 32 -- Thu, 20 Feb 2014 12:40:37 -0600 2, 32 -- X-IronPort-AV: E=Sophos;i="4.97,513,1389762000"; 2, 32 -- d="scan'208";a="38008255" 2, 32 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) 2, 32 -- by IPEX5.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 20 Feb 2014 13:40:37 -0500 2, 32 -- MIME-version: 1.0 2, 32 -- Content-transfer-encoding: 7BIT 2, 32 -- Content-disposition: inline 2, 32 -- Content-type: text/plain; CHARSET=US-ASCII 2, 32 -- Received: from johnshopkins.edu ([unknown] [10.173.7.15]) 2, 32 -- by commsmail.johnshopkins.edu 2, 32 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 2, 32 -- with ESMTP id {0N1B00J6457O8AB0-at-commsmail.johnshopkins.edu} ; Thu, 2, 32 -- 20 Feb 2014 13:40:36 -0500 (EST) 2, 32 -- Received: from [10.173.109.13] (Forwarded-For: [10.16.66.55]) 2, 32 -- by commsmail.johnshopkins.edu (mshttpd); Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 2, 32 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} , 2, 32 -- MSA listserver {Microscopy-at-msa.microscopy.com} , 2, 32 -- "NewSubList-at-Microscopy.com" {NewSubList-at-microscopy.com} 2, 32 -- Message-id: {72408ae2142e.530605d4-at-johnshopkins.edu} 2, 32 -- Date: Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 2, 32 -- 2009) 2, 32 -- Content-language: en 2, 32 -- Subject: HEPES and HPF 2, 32 -- X-Accept-Language: en 2, 32 -- Priority: normal ==============================End of - Headers==============================
Michael - I use HEPES routinely in my regular fix and for immunocytochemistry. I typically use 70 mM NaCl, 30 mM HEPES, 2 mM CaCl2, pH 7.4. I based my choice of osmolarity on the old half-strength Karnovsky's buffer which if I remember was 100 mM cacodylate. You see a lot of people referring to half-strength Karnovsky's as being ~1000 mOsm (or full-strength as being 2010 mOsm). These numbers assume the formaldehyde and glutaraldehyde contribute to the osmolarity of the fix. They might count in an osmometer measurement but if something crosses the membrane it doesn't count to the cell. My buffer is clearly "hypo-osmotic" to a mammalian cell at ~300 mOsm but empirically it seems to cause the least shrinkage or swelling. Your results may vary. Somebody once told me that Karnovsky picked a high aldehyde concentration to show they didn't contribute to the osmolarity but I don't know if that is true.
Is there a difference between 2% PF and 4% PF? Yes, I believe 4% has twice as much PF! Sorry, I couldn't resist the joke. I know exactly what you mean. I haven't seen much difference for cell lines and almost always use 2%. I use 2% PF by itself based on my use of "half-strength" Karnovsky's. The old "full-strength Karnovsky's fix was 4% PF and 5% glutaraldehyde - half-strength cut the aldehyde concentrations in half but not the buffer concentration. I suspect with an excess of aldehyde, the same amount of crosslinking occurs if you wait long enough.
Good luck. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu] Sent: Thursday, February 20, 2014 12:42 PM To: Phillips, Thomas E.
Dear Listservers, I am planning an HPF hybrid (pre-light chemical fixation) technique on attached cells, for HM-20 post embed IEM. My plan is to lightly fix the cells for 1 hr (4% PF 0.5% Ga 0.1M HEPES with 5mM MgCl2), rinse in 0.1M HEPES 3.5% sucrose with 5mM MgCl2 (243 mOsmols), then scrape and pellet the cells. I will resuspend the pellet in 2% agarose (in the cells media minus serum) just before HPF. My question is has anyone used HEPES as an IEM buffer, and at what concentration (molarity)? I have read that a slightly hypotonic solution is better for IEM, I believe the cultured cells are isotonic at 300 mOsmols. Also for IEM, is there really a difference between 4% PF and 2% PF? Any suggestions are welcome.
sincerely, Michael Delannoy
==============================Original Headers============================== 2, 32 -- From prvs=1211802d7=delannoy-at-jhmi.edu Thu Feb 20 12:40:37 2014 2, 32 -- Received: from IPEX5.johnshopkins.edu (ipex5.johnshopkins.edu [128.220.161.72]) 2, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1KIeb4e013212; 2, 32 -- Thu, 20 Feb 2014 12:40:37 -0600 2, 32 -- X-IronPort-AV: E=Sophos;i="4.97,513,1389762000"; 2, 32 -- d="scan'208";a="38008255" 2, 32 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) 2, 32 -- by IPEX5.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 20 Feb 2014 13:40:37 -0500 2, 32 -- MIME-version: 1.0 2, 32 -- Content-transfer-encoding: 7BIT 2, 32 -- Content-disposition: inline 2, 32 -- Content-type: text/plain; CHARSET=US-ASCII 2, 32 -- Received: from johnshopkins.edu ([unknown] [10.173.7.15]) 2, 32 -- by commsmail.johnshopkins.edu 2, 32 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 2, 32 -- with ESMTP id {0N1B00J6457O8AB0-at-commsmail.johnshopkins.edu} ; Thu, 2, 32 -- 20 Feb 2014 13:40:36 -0500 (EST) 2, 32 -- Received: from [10.173.109.13] (Forwarded-For: [10.16.66.55]) 2, 32 -- by commsmail.johnshopkins.edu (mshttpd); Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 2, 32 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} , 2, 32 -- MSA listserver {Microscopy-at-msa.microscopy.com} , 2, 32 -- "NewSubList-at-Microscopy.com" {NewSubList-at-microscopy.com} 2, 32 -- Message-id: {72408ae2142e.530605d4-at-johnshopkins.edu} 2, 32 -- Date: Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 2, 32 -- 2009) 2, 32 -- Content-language: en 2, 32 -- Subject: HEPES and HPF 2, 32 -- X-Accept-Language: en 2, 32 -- Priority: normal ==============================End of - Headers==============================
==============================Original Headers============================== 14, 32 -- From PhillipsT-at-missouri.edu Thu Feb 20 17:19:15 2014 14, 32 -- Received: from um-tip2-missouri-out.um.umsystem.edu (um-tip2-missouri-out.um.umsystem.edu [198.209.49.149]) 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1KNJEqM009106 14, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 17:19:15 -0600 14, 32 -- X-IronPort-Anti-Spam-Filtered: true 14, 32 -- X-IronPort-Anti-Spam-Result: AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQELAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP4Iq 14, 32 -- X-IPAS-Result: AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQELAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP4Iq 14, 32 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) 14, 32 -- by um-tip2-exch-relay.um.umsystem.edu with ESMTP; 20 Feb 2014 17:19:13 -0600 14, 32 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) by 14, 32 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi id 14.03.0174.001; 14, 32 -- Thu, 20 Feb 2014 17:19:13 -0600 14, 32 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 14, 32 -- To: "delannoy-at-jhmi.edu" {delannoy-at-jhmi.edu} , 14, 32 -- "'microscopy-at-microscopy.com'" 14, 32 -- {microscopy-at-microscopy.com} 14, 32 -- Subject: RE: [Microscopy] HEPES and HPF 14, 32 -- Thread-Topic: [Microscopy] HEPES and HPF 14, 32 -- Thread-Index: AQHPLmtlpsZ4x+1j6Eefm0lyGFg1L5q+wryg 14, 32 -- Date: Thu, 20 Feb 2014 23:19:13 +0000 14, 32 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8ED37E5-at-UM-MBX-T02.um.umsystem.edu} 14, 32 -- References: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} 14, 32 -- In-Reply-To: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} 14, 32 -- Accept-Language: en-US 14, 32 -- Content-Language: en-US 14, 32 -- X-MS-Has-Attach: 14, 32 -- X-MS-TNEF-Correlator: 14, 32 -- x-originating-ip: [128.206.81.115] 14, 32 -- Content-Type: text/plain; charset="us-ascii" 14, 32 -- MIME-Version: 1.0 14, 32 -- Content-Transfer-Encoding: 8bit 14, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1KNJEqM009106 ==============================End of - Headers==============================
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==============================Original Headers============================== 12, 29 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 20 18:13:46 2014 12, 29 -- Received: from znl.com ([206.69.208.20]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1L0DkLr028564 12, 29 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 18:13:46 -0600 12, 29 -- Received: from localhost (localhost [127.0.0.1]) 12, 29 -- by znl.com (Postfix) with ESMTP id 1CA596C200F 12, 29 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 18:13:46 -0600 (CST) 12, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 29 -- Received: from znl.com ([127.0.0.1]) 12, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 29 -- with ESMTP id 70urhNz2Hm4S for {microscopy-at-microscopy.com} ; 12, 29 -- Thu, 20 Feb 2014 18:13:42 -0600 (CST) 12, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 29 -- by znl.com (Postfix) with ESMTPA id D92886C2002 12, 29 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 18:13:42 -0600 (CST) 12, 29 -- Message-ID: {53069A36.8050208-at-microscopy.com} 12, 29 -- Date: Thu, 20 Feb 2014 18:13:42 -0600 12, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 29 -- MIME-Version: 1.0 12, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 29 -- Subject: viaWWW:An Analytical Scientist position open in BP Petrochemical 12, 29 -- Technology unit 12, 29 -- References: {201402201738.s1KHc26n012004-at-ns.microscopy.com} 12, 29 -- In-Reply-To: {201402201738.s1KHc26n012004-at-ns.microscopy.com} 12, 29 -- X-Forwarded-Message-Id: {201402201738.s1KHc26n012004-at-ns.microscopy.com} 12, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I thank Prof. Phillips for the thorough explanation of his opinion on the matter. I also would like to second use of 2%PFA (FA made from paraformaldehyde powder right before)instead of 4% for cell cultures/cultured cells. I have read papers stating 30 min. initial fixation ( -at-room temperature ) would be sufficient for cultured cells. Needless to say it would be of advantage to block unreacted aldehydes after fixation by washes in your buffer + 50mM NH4Cl (ROTH J et al, Enhancement of Structural Preservation and Immunohistochemical Staining in low temperature embedded pancreatic tissue, JHC 29,663-671(1981)
I have seen also an interesting post (on ResearchGate, www.researchgate.net, Thread: http://www.researchgate.net/topic/Histology/post/Alternatives_to_formalin_fixation Re by Thomas Kroneis . Medizinische Universität Graz = Med. Univ. Graz, Austria) on using HOPE(R) as an alternative for FA-fixation in IHC. HOPE. HOPE = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect T. Kroneis wrote in his post :
{ { ....Additionally to what has been posted above: there is another fixation around: HOPE. HOPE = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect (German: http://dcs-diagnostics.de; http://dcs-diagnostics.de/index.php?sprache=de ; ENGL: http://dcs-diagnostics.de/index.php?sprache=en ),
HOPE(R)-Fixation: GERM: http://dcs-diagnostics.de/index.php?sprache=de&&qid=48 , ENGL: http://dcs-diagnostics.de/index.php?sprache=en&&qid=63 ). This fixation yields as good morphological quality as FFPE material and circumvents the problem with the antibodies not recognizing the epitopes! Now the bad news: You need to place your sample directly into the pre-cooled HOPE I solution, second, it takes you quite some time (fixation 16h - 72h plus another day). - So if there is just no possibility to have it snap frozen, maybe it would be possible to have the respective solution stored there (crucial: at +2°C!). If you do not perform RNA analysis afterwards (do you?), you also might think of having the sample just stored in a tube and transferred to your site (in case of HOPE - just cooling, do not use buffers etc.). Maybe you should also think of getting solved the logical issue. Good science needs perfect conditions (you know: shit in - shit out!) Hope this helped, contact me, if you need further assistance (our lab protocol, ...)} } , and, same thread: Re by: Sebastian Marwitz . Research Center Borstel { { The probably best described alternative to PFA is the HOPE-technique: (Olert et al.,Pathol Res Pract. 2001;197(12):823-6); Wiedorn et al., Pathol Res Pract. 2002;198(11):735-40; Kähler et al., J Histochem Cytochem. 2010 Mar;58(3):221-8; Marwitz et al., J Histochem Cytochem. 2011 Jun;59(6):601-14.; Vollmer et al., Rom J Morphol Embryol. 2006;47(1):15-9; Goldmann et al., Pathol Res Pract. 2005;201(8-9):599-602.; Goldmann et al., Am J Pathol. 2003 Dec;163(6):2638-40) . It is a non-crosslinking fixation with acetone dehydration und subsequent paraffin-embedding. There is compared to PFA only minimal denaturation of nucleic acids and allows a multitude of read-outs from one tissue/cell block (qPCR, proteomics, RNA in situ hybridization, microarrays, ...). Most important, you don´t need any antigen-retrieval and are able to dilute your primary antibodies very high. If you are skilled in handling PFA-fixed specimen, there will be no big difference in tissue processing and cutting. End of citation} }
Also I have a pdf in my files, written by T.W. BRIMBLE: The developing Role of the Zwitterionic Buffer (1981) wherein use of HEPES buffer in various combinations or molar concentrations in Cell/organ culture, bacterial culture, blood proteins, cryogenic tissue storage and chemical data will be presented and discussed. Since at the beginning of the "era of zwitterionic buffers" there is only a small subtitle "EM" showing interesting effects in fixation of plant material (total 48 references, file= 5,5MB). So if anyone is interested, I could send that file to any requester..
Have a nice and warm weekend, especially in the US,
best regards, Wolfgang
Wolfgang MUSS EM-Lab Univ.Inst.Pathology SALK-LKH(Gen. Hosp.) and PMU (Private Paracelsus Medical University SALZBURG) SALZBURG-AUSTRIA DISCLAIMER: no affiliation, or financial interest in any of the companies or their products.
} -----Ursprüngliche Nachricht----- } Von: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] } Gesendet: Freitag, 21. Februar 2014 00:25 } An: Muß Wolfgang } Betreff: [Microscopy] RE: HEPES and HPF } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } (Re: PhillipsT-at-missouri.edu) } Michael - I use HEPES routinely in my regular fix and for } immunocytochemistry. I typically use 70 mM NaCl, 30 mM HEPES, 2 mM } CaCl2, pH 7.4. I based my choice of osmolarity on the old half-strength } Karnovsky's buffer which if I remember was 100 mM cacodylate. You see a } lot of people referring to half-strength Karnovsky's as being ~1000 } mOsm (or full-strength as being 2010 mOsm). These numbers assume the } formaldehyde and glutaraldehyde contribute to the osmolarity of the } fix. They might count in an osmometer measurement but if something } crosses the membrane it doesn't count to the cell. My buffer is clearly } "hypo-osmotic" to a mammalian cell at ~300 mOsm but empirically it } seems to cause the least shrinkage or swelling. Your results may vary. } Somebody once told me that Karnovsky picked a high aldehyde } concentration to show they didn't contribute to the osmolarity but I } don't know if that is true. } } Is there a difference between 2% PF and 4% PF? Yes, I believe 4% has } twice as much PF! Sorry, I couldn't resist the joke. I know exactly } what you mean. I haven't seen much difference for cell lines and almost } always use 2%. I use 2% PF by itself based on my use of "half-strength" } Karnovsky's. The old "full-strength Karnovsky's fix was 4% PF and 5% } glutaraldehyde - half-strength cut the aldehyde concentrations in half } but not the buffer concentration. I suspect with an excess of aldehyde, } the same amount of crosslinking occurs if you wait long enough. } } Good luck. Tom } } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://biology.missouri.edu/people/?person=88 } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu] } Sent: Thursday, February 20, 2014 12:42 PM } To: Phillips, Thomas E. } Subject: [Microscopy] HEPES and HPF } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Dear Listservers, } I am planning an HPF hybrid (pre-light chemical fixation) technique on } attached cells, for HM-20 post embed IEM. My plan is to lightly fix } the cells for 1 hr (4% PF 0.5% Ga 0.1M HEPES with 5mM MgCl2), rinse in } 0.1M HEPES 3.5% sucrose with 5mM MgCl2 (243 mOsmols), then scrape and } pellet the cells. I will resuspend the pellet in 2% agarose (in the } cells media minus serum) just before HPF. My question is has anyone } used HEPES as an IEM buffer, and at what concentration (molarity)? I } have read that a slightly hypotonic solution is better for IEM, I } believe the cultured cells are isotonic at 300 mOsmols. Also for IEM, } is there really a difference between 4% PF and 2% PF? Any suggestions } are welcome. } } sincerely, } Michael Delannoy } } ==============================Original } Headers============================== } 2, 32 -- From prvs=1211802d7=delannoy-at-jhmi.edu Thu Feb 20 12:40:37 2014 } 2, 32 -- Received: from IPEX5.johnshopkins.edu (ipex5.johnshopkins.edu } [128.220.161.72]) } 2, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s1KIeb4e013212; } 2, 32 -- Thu, 20 Feb 2014 12:40:37 -0600 } 2, 32 -- X-IronPort-AV: E=Sophos;i="4.97,513,1389762000"; } 2, 32 -- d="scan'208";a="38008255" } 2, 32 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) } 2, 32 -- by IPEX5.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 20 Feb } 2014 13:40:37 -0500 } 2, 32 -- MIME-version: 1.0 } 2, 32 -- Content-transfer-encoding: 7BIT 2, 32 -- Content-disposition: } inline 2, 32 -- Content-type: text/plain; CHARSET=US-ASCII 2, 32 -- } Received: from johnshopkins.edu ([unknown] [10.173.7.15]) 2, 32 -- by } commsmail.johnshopkins.edu 2, 32 -- (Sun Java(tm) System Messaging } Server 7u3-12.01 64bit (built Oct 15 2009)) 2, 32 -- with ESMTP id } {0N1B00J6457O8AB0-at-commsmail.johnshopkins.edu} ; Thu, 2, 32 -- 20 Feb } 2014 13:40:36 -0500 (EST) 2, 32 -- Received: from [10.173.109.13] } (Forwarded-For: [10.16.66.55]) 2, 32 -- by commsmail.johnshopkins.edu } (mshttpd); Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- From: Michael J } Delannoy {delannoy-at-jhmi.edu} 2, 32 -- To: "microscopy-at-microscopy.com" } {microscopy-at-microscopy.com} , } 2, 32 -- "microscopy-at-msa.microscopy.com" } {microscopy-at-msa.microscopy.com} , } 2, 32 -- MSA listserver {Microscopy-at-msa.microscopy.com} , } 2, 32 -- "NewSubList-at-Microscopy.com" } {NewSubList-at-microscopy.com} } 2, 32 -- Message-id: {72408ae2142e.530605d4-at-johnshopkins.edu} } 2, 32 -- Date: Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- X-Mailer: Sun } Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 2, 32 - } - 2009) 2, 32 -- Content-language: en 2, 32 -- Subject: HEPES and HPF } 2, 32 -- X-Accept-Language: en 2, 32 -- Priority: normal } ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 14, 32 -- From PhillipsT-at-missouri.edu Thu Feb 20 17:19:15 2014 } 14, 32 -- Received: from um-tip2-missouri-out.um.umsystem.edu (um-tip2- } missouri-out.um.umsystem.edu [198.209.49.149]) } 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s1KNJEqM009106 } 14, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 17:19:15 } -0600 } 14, 32 -- X-IronPort-Anti-Spam-Filtered: true } 14, 32 -- X-IronPort-Anti-Spam-Result: } AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQE } LAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP4 } Iq } 14, 32 -- X-IPAS-Result: } AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQE } LAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP4 } Iq } 14, 32 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) } 14, 32 -- by um-tip2-exch-relay.um.umsystem.edu with ESMTP; 20 Feb } 2014 17:19:13 -0600 } 14, 32 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) } by } 14, 32 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi id } 14.03.0174.001; } 14, 32 -- Thu, 20 Feb 2014 17:19:13 -0600 } 14, 32 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 14, 32 -- To: "delannoy-at-jhmi.edu" {delannoy-at-jhmi.edu} , } 14, 32 -- "'microscopy-at-microscopy.com'" } 14, 32 -- {microscopy-at-microscopy.com} } 14, 32 -- Subject: RE: [Microscopy] HEPES and HPF } 14, 32 -- Thread-Topic: [Microscopy] HEPES and HPF } 14, 32 -- Thread-Index: AQHPLmtlpsZ4x+1j6Eefm0lyGFg1L5q+wryg } 14, 32 -- Date: Thu, 20 Feb 2014 23:19:13 +0000 } 14, 32 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8ED37E5-at-UM-MBX- } T02.um.umsystem.edu} } 14, 32 -- References: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} } 14, 32 -- In-Reply-To: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} } 14, 32 -- Accept-Language: en-US } 14, 32 -- Content-Language: en-US } 14, 32 -- X-MS-Has-Attach: } 14, 32 -- X-MS-TNEF-Correlator: } 14, 32 -- x-originating-ip: [128.206.81.115] } 14, 32 -- Content-Type: text/plain; charset="us-ascii" } 14, 32 -- MIME-Version: 1.0 } 14, 32 -- Content-Transfer-Encoding: 8bit } 14, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s1KNJEqM009106 } ==============================End of - } Headers==============================
==============================Original Headers============================== 14, 38 -- From W.Muss-at-salk.at Fri Feb 21 03:39:09 2014 14, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 14, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1L9d8i8021016 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 03:39:08 -0600 14, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 14, 38 -- by hermes.salk.at (Postfix) with ESMTP id 3A988646B3F 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- X-Scanned-By: SALK-Content-Filter 14, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 14, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 14, 38 -- with ESMTP id QpnRWQ6Q869V for {microscopy-at-microscopy.com} ; 14, 38 -- Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 14, 38 -- by hermes.salk.at (Postfix) with ESMTP id 0AB3B646B39 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 14, 38 -- by N2EX199.lks.local with ESMTP; 21 Feb 2014 10:43:18 +0100 14, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 14, 38 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 14, 38 -- 14.02.0387.000; Fri, 21 Feb 2014 10:38:44 +0100 14, 38 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 14, 38 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 14, 38 -- Subject: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) 14, 38 -- Thread-Topic: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) 14, 38 -- Thread-Index: AQHPLpLyiInVfqdn5UaTlA2X/RENBpq/Z+oQ 14, 38 -- Date: Fri, 21 Feb 2014 09:38:43 +0000 14, 38 -- Message-ID: {3D22E6EE65F39E448DCE4162815376711C5FBCA4-at-N2DT131.lksdom21.lks.local} 14, 38 -- References: {201402202324.s1KNOY1E013491-at-ns.microscopy.com} 14, 38 -- In-Reply-To: {201402202324.s1KNOY1E013491-at-ns.microscopy.com} 14, 38 -- Accept-Language: de-DE, en-US 14, 38 -- Content-Language: de-DE 14, 38 -- X-MS-Has-Attach: 14, 38 -- X-MS-TNEF-Correlator: 14, 38 -- x-originating-ip: [192.168.42.2] 14, 38 -- Content-Type: text/plain; charset="iso-8859-1" 14, 38 -- MIME-Version: 1.0 14, 38 -- Content-Transfer-Encoding: 8bit 14, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1L9d8i8021016 ==============================End of - Headers==============================
A while ago while working on the histology of naturally distended rabbit urinary bladders that had been subjected to outlet obstruction, I used a (P)HCHO at 4% in a buffer (probably phosphate) at 4 degrees C as a 'quick' fix (10 minutes) of the whole, distended, bladder followed by removal of the urine and replacement with more cold buffered (P)HCHO to further fix the mucosal side of the bladder wall. The point here is that the 10 minute immersion 'froze' the smooth muscle so that the bladder did not contract when removing the urine and replacing with fixative.
This method worked as well on muscle that had been used in physiologic contraction studies to preclude contraction when the tissue was stimulated/studied in Hank's Balanced Salt Solution rather than in traditional phosphate or Tyrode buffer. The problem is that Hank's is not approved via general use, but if you want your muscle to look like muscle when you are done, something like Hank's is helpful.
As to the concentration of HCHO, it is my belief that all histologic protocols support/require the use of excesses of formaldehyde, and everything else. This is an edgy topic, and likely not worth too much time. The key issue with all methods of tissue preservation lies with the necessity to avoid having to provide an explanation for why one's tissue looks so bad!!! What IS/WAS liver must LOOK like liver!
Cheers, and hope this starts arguments,
Fred Monson.
What else does one do when one gets old other than get cranky?
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmonson-at-wcupa.edu
HomePage: http://cmirt.wcupa.edu (with link to instrument Scheduler)
-----Original Message----- X-from: W.Muss-at-salk.at [mailto:W.Muss-at-salk.at] Sent: Friday, February 21, 2014 4:49 AM To: Monson, Frederick
Dear Michael, dear all,
I thank Prof. Phillips for the thorough explanation of his opinion on the matter. I also would like to second use of 2%PFA (FA made from paraformaldehyde powder right before)instead of 4% for cell cultures/cultured cells. I have read papers stating 30 min. initial fixation ( -at-room temperature ) would be sufficient for cultured cells. Needless to say it would be of advantage to block unreacted aldehydes after fixation by washes in your buffer + 50mM NH4Cl (ROTH J et al, Enhancement of Structural Preservation and Immunohistochemical Staining in low temperature embedded pancreatic tissue, JHC 29,663-671(1981)
I have seen also an interesting post (on ResearchGate, www.researchgate.net, Thread: http://www.researchgate.net/topic/Histology/post/Alternatives_to_formalin_fixation Re by Thomas Kroneis . Medizinische Universität Graz = Med. Univ. Graz, Austria) on using HOPE(R) as an alternative for FA-fixation in IHC. HOPE. HOPE = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect T. Kroneis wrote in his post :
{ { ....Additionally to what has been posted above: there is another fixation around: HOPE. HOPE = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect (German: http://dcs-diagnostics.de; http://dcs-diagnostics.de/index.php?sprache=de ; ENGL: http://dcs-diagnostics.de/index.php?sprache=en ),
HOPE(R)-Fixation: GERM: http://dcs-diagnostics.de/index.php?sprache=de&&qid=48 , ENGL: http://dcs-diagnostics.de/index.php?sprache=en&&qid=63 ). This fixation yields as good morphological quality as FFPE material and circumvents the problem with the antibodies not recognizing the epitopes! Now the bad news: You need to place your sample directly into the pre-cooled HOPE I solution, second, it takes you quite some time (fixation 16h - 72h plus another day). - So if there is just no possibility to have it snap frozen, maybe it would be possible to have the respective solution stored there (crucial: at +2°C!). If you do not perform RNA analysis afterwards (do you?), you also might think of having the sample just stored in a tube and transferred to your site (in case of HOPE - just cooling, do not use buffers etc.). Maybe you should also think of getting solved the logical issue. Good science needs perfect conditions (you know: shit in - shit out!) Hope this helped, contact me, if you need further assistance (our lab protocol, ...)} } , and, same thread: Re by: Sebastian Marwitz . Research Center Borstel { { The probably best described alternative to PFA is the HOPE-technique: (Olert et al.,Pathol Res Pract. 2001;197(12):823-6); Wiedorn et al., Pathol Res Pract. 2002;198(11):735-40; Kähler et al., J Histochem Cytochem. 2010 Mar;58(3):221-8; Marwitz et al., J Histochem Cytochem. 2011 Jun;59(6):601-14.; Vollmer et al., Rom J Morphol Embryol. 2006;47(1):15-9; Goldmann et al., Pathol Res Pract. 2005;201(8-9):599-602.; Goldmann et al., Am J Pathol. 2003 Dec;163(6):2638-40) . It is a non-crosslinking fixation with acetone dehydration und subsequent paraffin-embedding. There is compared to PFA only minimal denaturation of nucleic acids and allows a multitude of read-outs from one tissue/cell block (qPCR, proteomics, RNA in situ hybridization, microarrays, ...). Most important, you don´t need any antigen-retrieval and are able to dilute your primary antibodies very high. If you are skilled in handling PFA-fixed specimen, there will be no big difference in tissue processing and cutting. End of citation} }
Also I have a pdf in my files, written by T.W. BRIMBLE: The developing Role of the Zwitterionic Buffer (1981) wherein use of HEPES buffer in various combinations or molar concentrations in Cell/organ culture, bacterial culture, blood proteins, cryogenic tissue storage and chemical data will be presented and discussed. Since at the beginning of the "era of zwitterionic buffers" there is only a small subtitle "EM" showing interesting effects in fixation of plant material (total 48 references, file= 5,5MB). So if anyone is interested, I could send that file to any requester..
Have a nice and warm weekend, especially in the US,
best regards, Wolfgang
Wolfgang MUSS EM-Lab Univ.Inst.Pathology SALK-LKH(Gen. Hosp.) and PMU (Private Paracelsus Medical University SALZBURG) SALZBURG-AUSTRIA DISCLAIMER: no affiliation, or financial interest in any of the companies or their products.
} -----Ursprüngliche Nachricht----- } Von: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] } Gesendet: Freitag, 21. Februar 2014 00:25 } An: Muß Wolfgang } Betreff: [Microscopy] RE: HEPES and HPF } } } } } ---------------------------------------------------------------------- } - } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } - } ----- } (Re: PhillipsT-at-missouri.edu) } Michael - I use HEPES routinely in my regular fix and for } immunocytochemistry. I typically use 70 mM NaCl, 30 mM HEPES, 2 mM } CaCl2, pH 7.4. I based my choice of osmolarity on the old } half-strength Karnovsky's buffer which if I remember was 100 mM } cacodylate. You see a lot of people referring to half-strength } Karnovsky's as being ~1000 mOsm (or full-strength as being 2010 } mOsm). These numbers assume the formaldehyde and glutaraldehyde } contribute to the osmolarity of the fix. They might count in an } osmometer measurement but if something crosses the membrane it doesn't } count to the cell. My buffer is clearly "hypo-osmotic" to a mammalian } cell at ~300 mOsm but empirically it seems to cause the least shrinkage or swelling. Your results may vary. } Somebody once told me that Karnovsky picked a high aldehyde } concentration to show they didn't contribute to the osmolarity but I } don't know if that is true. } } Is there a difference between 2% PF and 4% PF? Yes, I believe 4% has } twice as much PF! Sorry, I couldn't resist the joke. I know exactly } what you mean. I haven't seen much difference for cell lines and } almost always use 2%. I use 2% PF by itself based on my use of "half-strength" } Karnovsky's. The old "full-strength Karnovsky's fix was 4% PF and 5% } glutaraldehyde - half-strength cut the aldehyde concentrations in half } but not the buffer concentration. I suspect with an excess of } aldehyde, the same amount of crosslinking occurs if you wait long enough. } } Good luck. Tom } } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://biology.missouri.edu/people/?person=88 } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu] } Sent: Thursday, February 20, 2014 12:42 PM } To: Phillips, Thomas E. } Subject: [Microscopy] HEPES and HPF } } } } } ---------------------------------------------------------------------- } - } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } - } ----- } } Dear Listservers, } I am planning an HPF hybrid (pre-light chemical fixation) technique on } attached cells, for HM-20 post embed IEM. My plan is to lightly fix } the cells for 1 hr (4% PF 0.5% Ga 0.1M HEPES with 5mM MgCl2), rinse in } 0.1M HEPES 3.5% sucrose with 5mM MgCl2 (243 mOsmols), then scrape and } pellet the cells. I will resuspend the pellet in 2% agarose (in the } cells media minus serum) just before HPF. My question is has anyone } used HEPES as an IEM buffer, and at what concentration (molarity)? I } have read that a slightly hypotonic solution is better for IEM, I } believe the cultured cells are isotonic at 300 mOsmols. Also for IEM, } is there really a difference between 4% PF and 2% PF? Any suggestions } are welcome. } } sincerely, } Michael Delannoy } } ==============================Original } Headers============================== } 2, 32 -- From prvs=1211802d7=delannoy-at-jhmi.edu Thu Feb 20 12:40:37 } 2014 2, 32 -- Received: from IPEX5.johnshopkins.edu } (ipex5.johnshopkins.edu } [128.220.161.72]) } 2, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s1KIeb4e013212; } 2, 32 -- Thu, 20 Feb 2014 12:40:37 -0600 } 2, 32 -- X-IronPort-AV: E=Sophos;i="4.97,513,1389762000"; } 2, 32 -- d="scan'208";a="38008255" } 2, 32 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) } 2, 32 -- by IPEX5.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 20 Feb } 2014 13:40:37 -0500 } 2, 32 -- MIME-version: 1.0 } 2, 32 -- Content-transfer-encoding: 7BIT 2, 32 -- Content-disposition: } inline 2, 32 -- Content-type: text/plain; CHARSET=US-ASCII 2, 32 -- } Received: from johnshopkins.edu ([unknown] [10.173.7.15]) 2, 32 -- by } commsmail.johnshopkins.edu 2, 32 -- (Sun Java(tm) System Messaging } Server 7u3-12.01 64bit (built Oct 15 2009)) 2, 32 -- with ESMTP id } {0N1B00J6457O8AB0-at-commsmail.johnshopkins.edu} ; Thu, 2, 32 -- 20 Feb } 2014 13:40:36 -0500 (EST) 2, 32 -- Received: from [10.173.109.13] } (Forwarded-For: [10.16.66.55]) 2, 32 -- by commsmail.johnshopkins.edu } (mshttpd); Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- From: Michael J } Delannoy {delannoy-at-jhmi.edu} 2, 32 -- To: "microscopy-at-microscopy.com" } {microscopy-at-microscopy.com} , } 2, 32 -- "microscopy-at-msa.microscopy.com" } {microscopy-at-msa.microscopy.com} , } 2, 32 -- MSA listserver {Microscopy-at-msa.microscopy.com} , } 2, 32 -- "NewSubList-at-Microscopy.com" } {NewSubList-at-microscopy.com} } 2, 32 -- Message-id: {72408ae2142e.530605d4-at-johnshopkins.edu} } 2, 32 -- Date: Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- X-Mailer: Sun } Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 2, 32 } - } - 2009) 2, 32 -- Content-language: en 2, 32 -- Subject: HEPES and HPF } 2, 32 -- X-Accept-Language: en 2, 32 -- Priority: normal } ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 14, 32 -- From PhillipsT-at-missouri.edu Thu Feb 20 17:19:15 2014 14, 32 } -- Received: from um-tip2-missouri-out.um.umsystem.edu (um-tip2- } missouri-out.um.umsystem.edu [198.209.49.149]) } 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s1KNJEqM009106 } 14, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 17:19:15 } -0600 } 14, 32 -- X-IronPort-Anti-Spam-Filtered: true 14, 32 -- } X-IronPort-Anti-Spam-Result: } AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQ } E } LAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP } 4 } Iq } 14, 32 -- X-IPAS-Result: } AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQ } E } LAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP } 4 } Iq } 14, 32 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) } 14, 32 -- by um-tip2-exch-relay.um.umsystem.edu with ESMTP; 20 Feb } 2014 17:19:13 -0600 } 14, 32 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) } by 14, 32 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi } id 14.03.0174.001; 14, 32 -- Thu, 20 Feb 2014 17:19:13 -0600 14, 32 } -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 14, 32 -- To: } "delannoy-at-jhmi.edu" {delannoy-at-jhmi.edu} , } 14, 32 -- "'microscopy-at-microscopy.com'" } 14, 32 -- {microscopy-at-microscopy.com} } 14, 32 -- Subject: RE: [Microscopy] HEPES and HPF 14, 32 -- } Thread-Topic: [Microscopy] HEPES and HPF 14, 32 -- Thread-Index: } AQHPLmtlpsZ4x+1j6Eefm0lyGFg1L5q+wryg } 14, 32 -- Date: Thu, 20 Feb 2014 23:19:13 +0000 14, 32 -- Message-ID: } {CB463A8DE3499A4CA204087E4EEB363DD8ED37E5-at-UM-MBX- } T02.um.umsystem.edu} } 14, 32 -- References: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} } 14, 32 -- In-Reply-To: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} } 14, 32 -- Accept-Language: en-US } 14, 32 -- Content-Language: en-US } 14, 32 -- X-MS-Has-Attach: } 14, 32 -- X-MS-TNEF-Correlator: } 14, 32 -- x-originating-ip: [128.206.81.115] 14, 32 -- Content-Type: } text/plain; charset="us-ascii" } 14, 32 -- MIME-Version: 1.0 } 14, 32 -- Content-Transfer-Encoding: 8bit 14, 32 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s1KNJEqM009106 ==============================End } of - Headers==============================
==============================Original Headers============================== 14, 38 -- From W.Muss-at-salk.at Fri Feb 21 03:39:09 2014 14, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 14, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1L9d8i8021016 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 03:39:08 -0600 14, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 14, 38 -- by hermes.salk.at (Postfix) with ESMTP id 3A988646B3F 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- X-Scanned-By: SALK-Content-Filter 14, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 14, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 14, 38 -- with ESMTP id QpnRWQ6Q869V for {microscopy-at-microscopy.com} ; 14, 38 -- Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 14, 38 -- by hermes.salk.at (Postfix) with ESMTP id 0AB3B646B39 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 14, 38 -- by N2EX199.lks.local with ESMTP; 21 Feb 2014 10:43:18 +0100 14, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 14, 38 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 14, 38 -- 14.02.0387.000; Fri, 21 Feb 2014 10:38:44 +0100 14, 38 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 14, 38 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 14, 38 -- Subject: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) 14, 38 -- Thread-Topic: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) 14, 38 -- Thread-Index: AQHPLpLyiInVfqdn5UaTlA2X/RENBpq/Z+oQ 14, 38 -- Date: Fri, 21 Feb 2014 09:38:43 +0000 14, 38 -- Message-ID: {3D22E6EE65F39E448DCE4162815376711C5FBCA4-at-N2DT131.lksdom21.lks.local} 14, 38 -- References: {201402202324.s1KNOY1E013491-at-ns.microscopy.com} 14, 38 -- In-Reply-To: {201402202324.s1KNOY1E013491-at-ns.microscopy.com} 14, 38 -- Accept-Language: de-DE, en-US 14, 38 -- Content-Language: de-DE 14, 38 -- X-MS-Has-Attach: 14, 38 -- X-MS-TNEF-Correlator: 14, 38 -- x-originating-ip: [192.168.42.2] 14, 38 -- Content-Type: text/plain; charset="iso-8859-1" 14, 38 -- MIME-Version: 1.0 14, 38 -- Content-Transfer-Encoding: 8bit 14, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1L9d8i8021016 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 38 -- From FMonson-at-wcupa.edu Fri Feb 21 12:14:36 2014 28, 38 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.127.4]) 28, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1LIEZcB015735 28, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 12:14:35 -0600 28, 38 -- X-ASG-Debug-ID: 1393006473-0623962cca4f89e0001-4CH8be 28, 38 -- Received: from WCU-XCH-11.PASSHE.LCL (wcu-xch-01.wcupa.edu [144.26.0.85]) by MX01.WCUPA.EDU with ESMTP id khAnABQTHqBKZ5Dm (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 13:14:33 -0500 (EST) 28, 38 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 28, 38 -- X-Barracuda-Apparent-Source-IP: 144.26.0.85 28, 38 -- X-ASG-Whitelist: Client 28, 38 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 28, 38 -- WCU-XCH-11.PASSHE.LCL ([fe80::9034:edf3:907e:6463%13]) with mapi id 28, 38 -- 14.03.0123.003; Fri, 21 Feb 2014 13:14:33 -0500 28, 38 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 28, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 28, 38 -- Subject: RE: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) & 28, 38 -- bk to HCHO 28, 38 -- Thread-Topic: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) & 28, 38 -- bk to HCHO 28, 38 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) & 28, 38 -- bk to HCHO 28, 38 -- Thread-Index: Ac8vLRnbMiOIN/oNSAqok0EwiQUWQA== 28, 38 -- Date: Fri, 21 Feb 2014 18:14:33 +0000 28, 38 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C180BE923-at-WCU-XCH-03.PASSHE.LCL} 28, 38 -- Accept-Language: en-US 28, 38 -- Content-Language: en-US 28, 38 -- X-MS-Has-Attach: 28, 38 -- X-MS-TNEF-Correlator: 28, 38 -- x-originating-ip: [10.28.57.79] 28, 38 -- Content-Type: text/plain; charset="iso-8859-1" 28, 38 -- MIME-Version: 1.0 28, 38 -- X-Barracuda-Connect: wcu-xch-01.wcupa.edu[144.26.0.85] 28, 38 -- X-Barracuda-Start-Time: 1393006473 28, 38 -- X-Barracuda-Encrypted: AES128-SHA 28, 38 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 28, 38 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 28, 38 -- X-Barracuda-BRTS-Status: 1 28, 38 -- Content-Transfer-Encoding: 8bit 28, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1LIEZcB015735 ==============================End of - Headers==============================
From lytcinhu-at-natm.ru Sat Feb 22 04:37:38 2014 Return-Path: {lytcinhu-at-natm.ru} Received: from eth-24-236-81-78-homell.natm.ru ([78.81.236.24]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MAbaeO016449 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 22 Feb 2014 04:37:37 -0600 Message-Id: {201402221037.s1MAbaeO016449-at-ns.microscopy.com}
X-from: wul-at-mail.montclair.edu ()
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wul-at-mail.montclair.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: wul-at-mail.montclair.edu Name: Laying Wu
Organization: education
Title-Subject: [Filtered] Microscopy training workshop 2014
Message: Dear Listservers,
Montclair State University and Hitachi High Technologies Inc. are pleased to announce a low-cost, one-day Light and Electron Microscopy workshop for new and intermediate-level microscopists. The workshop will take place on Friday, March 21, 2014 at Montclair State University, Montclair New Jersey. The workshop will introduce participants to a wide range of the techniques associated with light microscopy, electron microscopy, atomic force microscopy and sample preparation. Guest presenters will join us from Hitachi High Technologies of America, Bruker AXS Inc., and Hitech Instruments Inc. Students, faculty, researchers, and industry users working in biology, geology, environmental science and material science are encouraged to apply.
Please visit the workshop website for further details and registration instructions: http://www.montclair.edu/csam/mmrl/workshop/
Montclair State University is located in Montclair, NJ. We are reachable via the New Jersey Transit train system (Montclair Heights and Montclair State University stations) and by bus. Please visit us at www.montclair.edu.
with best wishes,
Laying Wu
wul-at-mail.montclair.edu TEL: 973-655-2028
Dr. Laying Wu Director, Microscopy and Microanalysis Research Laboratory Montclair State University 1 Normal Ave, Montclair, NJ 07043 USA
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==============================Original Headers============================== 23, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 22 07:47:31 2014 23, 28 -- Received: from znl.com ([206.69.208.20]) 23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MDlVC4019191 23, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:47:31 -0600 23, 28 -- Received: from localhost (localhost [127.0.0.1]) 23, 28 -- by znl.com (Postfix) with ESMTP id C65936C338B 23, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:47:31 -0600 (CST) 23, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 23, 28 -- Received: from znl.com ([127.0.0.1]) 23, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 23, 28 -- with ESMTP id liUjiT8CLSrH for {microscopy-at-microscopy.com} ; 23, 28 -- Sat, 22 Feb 2014 07:47:27 -0600 (CST) 23, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 23, 28 -- by znl.com (Postfix) with ESMTPA id E77C76C3379 23, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:47:27 -0600 (CST) 23, 28 -- Message-ID: {5308AA6F.1060707-at-microscopy.com} 23, 28 -- Date: Sat, 22 Feb 2014 07:47:27 -0600 23, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 23, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 23, 28 -- MIME-Version: 1.0 23, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 23, 28 -- Subject: viaWWW: Microscopy training workshop 2014 at Montclair State University 23, 28 -- References: {201402211954.s1LJsKPO003592-at-ns.microscopy.com} 23, 28 -- In-Reply-To: {201402211954.s1LJsKPO003592-at-ns.microscopy.com} 23, 28 -- X-Forwarded-Message-Id: {201402211954.s1LJsKPO003592-at-ns.microscopy.com} 23, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 23, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both doug.skinner-at-bruker-nano.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Job Opportunity at Bruker - Applications Specialist
Message: Bruker Nano Analytics, a leading manufacturer of Analytical Instrumentation, has a job opening for an Applications Specialist in their Billerica, MA Microanalysis Lab. This division specializes in EDS, WDS, micro-XRF and EBSD on electron microscopes.
The Applications Specialist position is a Sales, Service and Customer support position. The responsibilities are to assist customers, demonstrate the microanalysis instruments and to provide technical sales support for the Microanalysis Product Line.
Primary Responsibilities: * Demonstrate equipment at Bruker and customer sites * Analysis of customer samples * Customer support on-site and via phone/e-mail * User training of the Bruker Microanalysis System * Produce appropriate materials for customer training * Preparation of technical sales materials * Assist Service in resolving customer problems * Perform other tasks as assigned by manager
This position involves up to 50% travel, primarily in North America and Latin America.
Candidates interested in this position require a minimum of a BachelorÂ’s degree in a Physical Science discipline with a degree in Chemistry, Geology or Materials Science preferred. Two years SEM/X-ray microanalysis and EBSD experience, including sample preparation, is preferred.
Requirements include excellent communication skills and proficiency with Microsoft Office. Language: English fluent; preferably Spanish as a second language.
Interested candidates should complete an online application at: www.bruker.com
Bruker Nano Analytics offers a competitive salary and comprehensive benefits package including health and dental insurance, Flexible Spending Account, company sponsored life and disability insurance, 401(k) plan with company matching components, and vacation and sick days.
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 22 07:48:34 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MDmX6M019878 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:48:33 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id B890A6C339A 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:48:33 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id lhsjDKxwAX7u for {microscopy-at-microscopy.com} ; 19, 28 -- Sat, 22 Feb 2014 07:48:31 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 2D90E6C338D 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:48:31 -0600 (CST) 19, 28 -- Message-ID: {5308AAAF.90908-at-microscopy.com} 19, 28 -- Date: Sat, 22 Feb 2014 07:48:31 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:Job Opportunity at Bruker - Applications Specialist 19, 28 -- References: {201402212101.s1LL1hT9004383-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201402212101.s1LL1hT9004383-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201402212101.s1LL1hT9004383-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 19, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Dear Colleagues, We would like to bring your attention to the call for abstracts: https://www.avs.org/Meetings-Exhibits/Call-For-Abstracts to the AVS 61st International Symposium & Exhibition (AVS-61), which will be held November 9-14, 2014 in Baltimore, Maryland. It is a good opportunity to explore and report on new developments in microscopy techniques applied to new materials, devices and interfaces. In addition, on the behalf of the organizers, we invite you to submit an abstract before the deadline of Monday, May 5th, to the special topical session "Novel Trends in Synchrotron and FEL-Based Analysis" https://www.avs.org/Meetings-Exhibits/Call-For-Abstracts/Focus-Topics#Synchrotron. This focus session is organized for the first time and aims at dissemination of the unique research opportunities, offered by the advanced experimental techniques at X-ray, VUV or IR synchrotron and free electron laser facilities, to investigate the properties of matter with unprecedented space, spectral and time resolution. One or two more invited presentation will be selected by the program committee evaluating the abstracts. We look forward to receiving your abstract and seeing you November in Baltimore, Maryland! _______________________________________________________________________________ Andrei Kolmakov, PhD Project Leader Center for Nanoscale Science and Technology National Institute of Standards and Technology
Folks - I have two Gatan 600 ion-mills that are up for grabs on Long Island, NY. Both are not 100% working, put contain 90% of parts. Together, you can easily make a working unit. You would have to come here yourself and load (or crate) them yourself.
If nobody offers to take them in their entirety, then anyone is welcome to come here to strip them for parts. I am out that business.
Either way, they are being surplussed in one month.
Next, does anyone have a PDF copy of the instruction manual and schematics for a Gatan 691 ion-mill? thank you and regards, - Jim
==============================Original Headers============================== 4, 26 -- From jquinn11733-at-gmail.com Sat Feb 22 16:10:07 2014 4, 26 -- Received: from mail-la0-f54.google.com (mail-la0-f54.google.com [209.85.215.54]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MMA1mO019338 4, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 16:10:01 -0600 4, 26 -- Received: by mail-la0-f54.google.com with SMTP id mc6so376969lab.27 4, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=20120113; 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 26 -- bh=5t1qAZth4wl4XiRf5nbs2fkeJMdtnubmtEwJi0ZW6bg=; 4, 26 -- b=tpP4pcWiJC0E3IvCxTik4bV6NyQJI8i6RDVWbDE1H3MGWSP+jhxc+fJYbNyvUsFcc/ 4, 26 -- +f0yVsKIO0r53pOsVRONcwG9MMvk+0dlr/ZC0sF8rbCiBoxS0HtyVLQVefZmQlm/VKyN 4, 26 -- MKe3XRKOq0YZ8jpKZCtY9Sxa5b6tt8vkUKkSmcp/2EqZnpoa3E7ghxEQLYkRKFGeryDb 4, 26 -- SJkfdAmU9donJwhgOWFtJdLZb0hBVvnUI467WJmws7SE8mPR0emb84S26RfSH9dJlkwq 4, 26 -- RKVbeHZ7Xy6bbLm4yHWCnj6KB1Ggu4hAtWYV9//yi9J/6oB8XMMW7FYADDbFDMeKltHO 4, 26 -- zXlA== 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Received: by 10.152.205.11 with SMTP id lc11mr7996429lac.29.1393107000474; 4, 26 -- Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- Received: by 10.112.218.3 with HTTP; Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- Date: Sat, 22 Feb 2014 17:10:00 -0500 4, 26 -- Message-ID: {CAO_SM8Kk_7H504-iMRzp+2-eY5tcQZXje=LD6JCp2cneKFNBZQ-at-mail.gmail.com} 4, 26 -- Subject: Gatan 600 ion-mills up for grabs and need manual for 691 4, 26 -- From: Jim Quinn {jquinn11733-at-gmail.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Folks - I have two Gatan 600 ion-mills that are up for grabs on Long Island, NY. Both are not 100% working, put contain 90% of parts. Together, you can easily make a working unit. You would have to come here yourself and load (or crate) them yourself.
If nobody offers to take them in their entirety, then anyone is welcome to come here to strip them for parts. I am out that business.
Either way, they are being surplussed in one month.
Next, does anyone have a PDF copy of the instruction manual and schematics for a Gatan 691 ion-mill? thank you and regards, - Jim
==============================Original Headers============================== 4, 26 -- From jquinn11733-at-gmail.com Sat Feb 22 18:02:24 2014 4, 26 -- Received: from mail-la0-f54.google.com (mail-la0-f54.google.com [209.85.215.54]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MMA1mO019338 4, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 16:10:01 -0600 4, 26 -- Received: by mail-la0-f54.google.com with SMTP id mc6so376969lab.27 4, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=20120113; 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 26 -- bh=5t1qAZth4wl4XiRf5nbs2fkeJMdtnubmtEwJi0ZW6bg=; 4, 26 -- b=tpP4pcWiJC0E3IvCxTik4bV6NyQJI8i6RDVWbDE1H3MGWSP+jhxc+fJYbNyvUsFcc/ 4, 26 -- +f0yVsKIO0r53pOsVRONcwG9MMvk+0dlr/ZC0sF8rbCiBoxS0HtyVLQVefZmQlm/VKyN 4, 26 -- MKe3XRKOq0YZ8jpKZCtY9Sxa5b6tt8vkUKkSmcp/2EqZnpoa3E7ghxEQLYkRKFGeryDb 4, 26 -- SJkfdAmU9donJwhgOWFtJdLZb0hBVvnUI467WJmws7SE8mPR0emb84S26RfSH9dJlkwq 4, 26 -- RKVbeHZ7Xy6bbLm4yHWCnj6KB1Ggu4hAtWYV9//yi9J/6oB8XMMW7FYADDbFDMeKltHO 4, 26 -- zXlA== 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Received: by 10.152.205.11 with SMTP id lc11mr7996429lac.29.1393107000474; 4, 26 -- Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- Received: by 10.112.218.3 with HTTP; Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- Date: Sat, 22 Feb 2014 17:10:00 -0500 4, 26 -- Message-ID: {CAO_SM8Kk_7H504-iMRzp+2-eY5tcQZXje=LD6JCp2cneKFNBZQ-at-mail.gmail.com} 4, 26 -- Subject: Gatan 600 ion-mills up for grabs and need manual for 691 4, 26 -- From: Jim Quinn {jquinn11733-at-gmail.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jcsmtf-at-mail.missouri.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: I am receiving a Pneumatics message on the screen of our CM10 and I believe this to be the reason while none of the vacuums will start. The air line on our machine runs first to V11, but it has not been allowed past V11. Is there a reason for this? I'm not sure how this valve works, I took it off and it seems to be just a metal unit with no moving parts. I'm guessing the black electrical piece that connects to it has something to do with its operations. Does anyone have any insight on how to open this valve? I am running ~90 psi to it.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Sun Feb 23 08:57:20 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1NEvKo2014694 12, 28 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 08:57:20 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id F2F186C40A5 12, 28 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 08:57:19 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id xJNd9kWLSO3n for {microscopy-at-microscopy.com} ; 12, 28 -- Sun, 23 Feb 2014 08:57:12 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id AECB96C4093 12, 28 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 08:57:12 -0600 (CST) 12, 28 -- Message-ID: {530A0C47.40906-at-microscopy.com} 12, 28 -- Date: Sun, 23 Feb 2014 08:57:11 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:CM10 pneumatics 12, 28 -- References: {201402230100.s1N10rDF021223-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201402230100.s1N10rDF021223-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201402230100.s1N10rDF021223-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Folks - Those two ion-mills are spoken for. Also, I now have the manual for the 691. Thank you Justin and Mike! regards, - Jim
} Folks - } I have two Gatan 600 ion-mills that } are up for grabs on Long Island, NY. } Both are not 100% working, put } contain 90% of parts. Together, } you can easily make a working unit. } You would have to come here yourself } and load (or crate) them yourself. } If nobody offers to take them in their entirety, } then anyone is welcome to come here to } strip them for parts. I am out that business. } Either way, they are being surplussed in one month. } Next, does anyone have a PDF copy of the } instruction manual and schematics for a Gatan 691 } ion-mill? } thank you and regards, } - Jim
==============================Original Headers============================== 3, 26 -- From jquinn11733-at-gmail.com Sun Feb 23 14:28:46 2014 3, 26 -- Received: from mail-la0-f46.google.com (mail-la0-f46.google.com [209.85.215.46]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1NKSkZ6006324 3, 26 -- for {Microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 14:28:46 -0600 3, 26 -- Received: by mail-la0-f46.google.com with SMTP id b8so4575577lan.5 3, 26 -- for {Microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 12:28:45 -0800 (PST) 3, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=20120113; 3, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 26 -- bh=ZS4IwwHqcbgMhJDyqGVJCnleecfyQ7cYVorvgq1PH1c=; 3, 26 -- b=0ADkBfsoL3MIgUCBjH+megm3BV3Xcp+Sl3zwHWU5jpTu3RBh9haYLGwvdd6+1WWFXx 3, 26 -- ckW1JZsgd2Obsb4lxbmCp+LJxYLFUA9+zaxmw8z4T7ZcUM9NWXJBaWB/ESAp7KXWKTxT 3, 26 -- tLo+V1xZmtDvnB8RTk5nJYdOaw7YdGA+q7T7RrZ/Kq/I9Wvu16yf8QoY5AqTgtNFvEYW 3, 26 -- /OYdK+cLEgQuIblm27yNTU/2+mx5YzcAonhav+fyCO9sfSx8o4s112Bzc9/kYo7oL2kG 3, 26 -- DoMC6X74+Nsc8ZTgXoOckeKkITJ12qUzPUzJ73f89ErP0jCZU6EbgquE74FV0mT+Wq9P 3, 26 -- FtUA== 3, 26 -- MIME-Version: 1.0 3, 26 -- X-Received: by 10.153.11.129 with SMTP id ei1mr10054261lad.78.1393187325420; 3, 26 -- Sun, 23 Feb 2014 12:28:45 -0800 (PST) 3, 26 -- Received: by 10.112.218.3 with HTTP; Sun, 23 Feb 2014 12:28:45 -0800 (PST) 3, 26 -- Date: Sun, 23 Feb 2014 15:28:45 -0500 3, 26 -- Message-ID: {CAO_SM8KzJZfTY_hz+maALJ-uQ4a+WGSaVFVnm_242=hHyJPf0g-at-mail.gmail.com} 3, 26 -- Subject: ion-mills are now spoken for 3, 26 -- From: Jim Quinn {jquinn11733-at-gmail.com} 3, 26 -- To: Microscopy-at-microscopy.com 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
The microscope air supply should *NOT* be attached to V11. V11 is the microscope vent valve and is used to release the vacuum in the column and camera chamber. It should never have more than a fraction of a psi supplied to it. Higher pressure can force the viewing chamber window out with great force and cause serious injury to anyone nearby! Venting the column with high pressure air when the sample rod is inserted can also blow the sample rod out with some force and damage it.
The air for the valves is supplied through the "Watts" regulator to the distribution block on the rear of the microscope column. This air ACTIVATES the valves; it doesn't pass through the valve.
Cheers, Henk
On 2/23/2014 9:59 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: jcsmtf-at-mail.missouri.edu () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both jcsmtf-at-mail.missouri.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10 pneumatics } } Message: I am receiving a Pneumatics message on the screen of our CM10 and I believe this to be the } reason while none of the vacuums will start. The air line on our machine runs first to V11, but it } has not been allowed past V11. Is there a reason for this? I'm not sure how this valve works, I took } it off and it seems to be just a metal unit with no moving parts. I'm guessing the black electrical } piece that connects to it has something to do with its operations. Does anyone have any insight on } how to open this valve? I am running ~90 psi to it. } } Login Host: 161.130.188.19 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } ---===[|]===--- } } }
==============================Original Headers============================== 8, 47 -- From colijn.1-at-osu.edu Sun Feb 23 15:19:41 2014 8, 47 -- Received: from va3outboundpool.messaging.microsoft.com (va3ehsobe010.messaging.microsoft.com [216.32.180.30]) 8, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1NLJfFZ002234 8, 47 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 15:19:41 -0600 8, 47 -- Received: from mail211-va3-R.bigfish.com (10.7.14.225) by 8, 47 -- VA3EHSOBE012.bigfish.com (10.7.40.62) with Microsoft SMTP Server id 8, 47 -- 14.1.225.22; Sun, 23 Feb 2014 21:19:41 +0000 8, 47 -- Received: from mail211-va3 (localhost [127.0.0.1]) by 8, 47 -- mail211-va3-R.bigfish.com (Postfix) with ESMTP id 267679801EC for 8, 47 -- {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 21:19:41 +0000 (UTC) 8, 47 -- X-Forefront-Antispam-Report: CIP:164.107.81.212;KIP:(null);UIP:(null);IPV:NLI;H:cio-krc-pf05;RD:none;EFVD:NLI 8, 47 -- X-SpamScore: 2 8, 47 -- X-BigFish: VPS2(zzbb2dI62a3I98dI9371I1432Izz1f42h2148h1d77h208ch1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch1fc6hzd9hz1de098h177df4h17326ah8275bh1de097h186068hz2fh2a8h839h947hd25he5bhf0ah1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh162dh1631h1758h1765h18e1h190ch1946h19b4h19c3h19ceh1ad9h1b0ah224fh1d0ch1d2eh1d3fh1dfeh1dffh1f5fh1fe8h1ff5h209eh21e4h22d0h2336h2438h2461h2476h2487h24d7h2516h2545h255eh1b1cn1b1bi1155h) 8, 47 -- Received-SPF: pass (mail211-va3: domain of osu.edu designates 164.107.81.212 as permitted sender) client-ip=164.107.81.212; envelope-from=colijn.1-at-osu.edu; helo=cio-krc-pf05 ;cio-krc-pf05 ; 8, 47 -- Received: from mail211-va3 (localhost.localdomain [127.0.0.1]) by mail211-va3 8, 47 -- (MessageSwitch) id 1393190378901058_23421; Sun, 23 Feb 2014 21:19:38 +0000 8, 47 -- (UTC) 8, 47 -- Received: from VA3EHSMHS031.bigfish.com (unknown [10.7.14.226]) by 8, 47 -- mail211-va3.bigfish.com (Postfix) with ESMTP id CD13D80064; Sun, 23 Feb 2014 8, 47 -- 21:19:38 +0000 (UTC) 8, 47 -- Received: from cio-krc-pf05 (164.107.81.212) by VA3EHSMHS031.bigfish.com 8, 47 -- (10.7.99.41) with Microsoft SMTP Server (TLS) id 14.16.227.3; Sun, 23 Feb 8, 47 -- 2014 21:19:33 +0000 8, 47 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) (using TLSv1 8, 47 -- with cipher RC4-MD5 (128/128 bits)) (No client certificate requested) by 8, 47 -- cio-krc-pf05 (Postfix) with ESMTPS id F0DEA60056; Sun, 23 Feb 2014 16:19:32 8, 47 -- -0500 (EST) 8, 47 -- Received: from [192.168.1.102] (75.118.135.65) by smtp.service.osu.edu 8, 47 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.174.1; Sun, 23 Feb 8, 47 -- 2014 16:19:32 -0500 8, 47 -- Message-ID: {530A65E0.7020808-at-osu.edu} 8, 47 -- Date: Sun, 23 Feb 2014 16:19:28 -0500 8, 47 -- From: Henk Colijn {colijn.1-at-osu.edu} 8, 47 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 8, 47 -- MIME-Version: 1.0 8, 47 -- To: {microscopy-at-microscopy.com} , {jcsmtf-at-mail.missouri.edu} 8, 47 -- Subject: Re: [Microscopy] viaWWW:CM10 pneumatics 8, 47 -- References: {201402231459.s1NExkMp016345-at-ns.microscopy.com} 8, 47 -- In-Reply-To: {201402231459.s1NExkMp016345-at-ns.microscopy.com} 8, 47 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 8, 47 -- Content-Transfer-Encoding: 7bit 8, 47 -- X-Originating-IP: [75.118.135.65] 8, 47 -- X-CFilter-Loop: Forward 8, 47 -- X-FOPE-CRA-Verdict: 164.107.81.212$mail.missouri.edu%0%1%osu.edu%False%False%0$ 8, 47 -- X-OriginatorOrg: osu.edu 8, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 8, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%MAIL.MISSOURI.EDU$RO%1$TLS%0$FQDN%$TlsDn% ==============================End of - Headers==============================
The microscope air supply should *NOT* be attached to V11. V11 is the microscope vent valve and is used to release the vacuum in the column and camera chamber. It should never have more than a fraction of a psi supplied to it. Higher pressure can force the viewing chamber window out with great force and cause serious injury to anyone nearby! Venting the column with high pressure air when the sample rod is inserted can also blow the sample rod out with some force and damage it.
The air for the valves is supplied through the "Watts" regulator to the distribution block on the rear of the microscope column. This air ACTIVATES the valves; it doesn't pass through the valve.
Cheers, Henk
On 2/23/2014 9:59 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: jcsmtf-at-mail.missouri.edu () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both jcsmtf-at-mail.missouri.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10 pneumatics } } Message: I am receiving a Pneumatics message on the screen of our CM10 and I believe this to be the } reason while none of the vacuums will start. The air line on our machine runs first to V11, but it } has not been allowed past V11. Is there a reason for this? I'm not sure how this valve works, I took } it off and it seems to be just a metal unit with no moving parts. I'm guessing the black electrical } piece that connects to it has something to do with its operations. Does anyone have any insight on } how to open this valve? I am running ~90 psi to it. } } Login Host: 161.130.188.19 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } ---===[|]===--- } } }
==============================Original Headers============================== 8, 47 -- From colijn.1-at-osu.edu Sun Feb 23 15:19:52 2014 8, 47 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1ehsobe006.messaging.microsoft.com [216.32.181.186]) 8, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1NLJqrO002523 8, 47 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 15:19:52 -0600 8, 47 -- Received: from mail151-ch1-R.bigfish.com (10.43.68.251) by 8, 47 -- CH1EHSOBE021.bigfish.com (10.43.70.78) with Microsoft SMTP Server id 8, 47 -- 14.1.225.22; Sun, 23 Feb 2014 21:19:52 +0000 8, 47 -- Received: from mail151-ch1 (localhost [127.0.0.1]) by 8, 47 -- mail151-ch1-R.bigfish.com (Postfix) with ESMTP id D9A711A036E for 8, 47 -- {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 21:19:51 +0000 (UTC) 8, 47 -- X-Forefront-Antispam-Report: CIP:164.107.81.218;KIP:(null);UIP:(null);IPV:NLI;H:cio-tnc-pf04;RD:none;EFVD:NLI 8, 47 -- X-SpamScore: 2 8, 47 -- X-BigFish: VPS2(zzbb2dI62a3I98dI9371I1432Izz1f42h2148h1d77h208ch1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch1fc6hzd9hz1de098h177df4h17326ah8275bh1de097h186068hz2fh2a8h839h947hd25he5bhf0ah1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh162dh1631h1758h1765h18e1h190ch1946h19b4h19c3h19ceh1ad9h1b0ah224fh1d0ch1d2eh1d3fh1dfeh1dffh1f5fh1fe8h1ff5h209eh22d0h2336h2438h2461h2476h2487h24d7h2516h2545h255eh1b1cn1b1bi1155h) 8, 47 -- Received-SPF: pass (mail151-ch1: domain of osu.edu designates 164.107.81.218 as permitted sender) client-ip=164.107.81.218; envelope-from=colijn.1-at-osu.edu; helo=cio-tnc-pf04 ;cio-tnc-pf04 ; 8, 47 -- Received: from mail151-ch1 (localhost.localdomain [127.0.0.1]) by mail151-ch1 8, 47 -- (MessageSwitch) id 1393190390194971_11380; Sun, 23 Feb 2014 21:19:50 +0000 8, 47 -- (UTC) 8, 47 -- Received: from CH1EHSMHS019.bigfish.com (snatpool3.int.messaging.microsoft.com 8, 47 -- [10.43.68.229]) by mail151-ch1.bigfish.com (Postfix) with ESMTP id 8, 47 -- 16C744004F; Sun, 23 Feb 2014 21:19:50 +0000 (UTC) 8, 47 -- Received: from cio-tnc-pf04 (164.107.81.218) by CH1EHSMHS019.bigfish.com 8, 47 -- (10.43.70.19) with Microsoft SMTP Server (TLS) id 14.16.227.3; Sun, 23 Feb 8, 47 -- 2014 21:19:47 +0000 8, 47 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) (using TLSv1 8, 47 -- with cipher RC4-MD5 (128/128 bits)) (No client certificate requested) by 8, 47 -- cio-tnc-pf04 (Postfix) with ESMTPS id D0190380057; Sun, 23 Feb 2014 16:19:46 8, 47 -- -0500 (EST) 8, 47 -- Received: from [192.168.1.102] (75.118.135.65) by smtp.service.osu.edu 8, 47 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.174.1; Sun, 23 Feb 8, 47 -- 2014 16:19:46 -0500 8, 47 -- Message-ID: {530A65ED.6030007-at-osu.edu} 8, 47 -- Date: Sun, 23 Feb 2014 16:19:41 -0500 8, 47 -- From: Henk Colijn {colijn.1-at-osu.edu} 8, 47 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 8, 47 -- MIME-Version: 1.0 8, 47 -- To: {microscopy-at-microscopy.com} , {jcsmtf-at-mail.missouri.edu} 8, 47 -- Subject: Re: [Microscopy] viaWWW:CM10 pneumatics 8, 47 -- References: {201402231459.s1NExkMp016345-at-ns.microscopy.com} 8, 47 -- In-Reply-To: {201402231459.s1NExkMp016345-at-ns.microscopy.com} 8, 47 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 8, 47 -- Content-Transfer-Encoding: 7bit 8, 47 -- X-Originating-IP: [75.118.135.65] 8, 47 -- X-CFilter-Loop: Forward 8, 47 -- X-FOPE-CRA-Verdict: 164.107.81.218$mail.missouri.edu%0%1%osu.edu%False%False%0$ 8, 47 -- X-OriginatorOrg: osu.edu 8, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 8, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%MAIL.MISSOURI.EDU$RO%1$TLS%0$FQDN%$TlsDn% ==============================End of - Headers==============================
We have a very old SEM XL20 (FEI, runs under Windows 3.11, engl. version). Our hard disk crashed and we don't have a backup.
We have replaced the hard disk and got MS DOS and Win 3.11. installed, but we have problems with the operating system for the SEM. FEI is not able to help us, because the XL20 is so old.
Thus we are looking for: - a backup of the XL20 operating system or - XL20 - software V 5.24 (on discs or CD-ROM) including driver software or - EZ-SCSI-Driver (v3). (ADAPTEC-SCSI-SOFTWARE) or - MACH 64- Driver or - ATI-Driver for Win 3.11 (engl. Version).
Sincerely, Elisabeth Liebler-Tenorio
==============================Original Headers============================== 9, 29 -- From Elisabeth.Liebler-Tenorio-at-fli.bund.de Wed Feb 26 04:57:35 2014 9, 29 -- Received: from mx022.fli.bund.de (mx022.fli.bund.de [193.22.112.22]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QAvYMK027973 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Feb 2014 04:57:34 -0600 9, 29 -- Received: from FLI-RIE-HT039.intern.fli.bund.local (Not Verified[172.20.0.39]) by mx022.fli.bund.de with MailMarshal (v7,2,2,6606) 9, 29 -- id {B530dc8900000} ; Wed, 26 Feb 2014 11:57:20 +0100 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local (172.18.0.31) by 9, 29 -- FLI-RIE-HT039.intern.fli.bund.local (172.20.0.39) with Microsoft SMTP Server 9, 29 -- (TLS) id 14.3.174.1; Wed, 26 Feb 2014 11:57:34 +0100 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20]) 9, 29 -- by FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20%10]) with 9, 29 -- mapi id 14.03.0174.001; Wed, 26 Feb 2014 11:57:33 +0100 9, 29 -- From: "Liebler-Tenorio, Elisabeth" {Elisabeth.Liebler-Tenorio-at-fli.bund.de} 9, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 9, 29 -- CC: "ethalmann-at-t-online.de" {ethalmann-at-t-online.de} 9, 29 -- Subject: SEM XL20 (FEI) operating system/drivers 9, 29 -- Thread-Topic: SEM XL20 (FEI) operating system/drivers 9, 29 -- Thread-Index: Ac8y4WJQgviqvi0tQz6N3f8e25e20g== 9, 29 -- Date: Wed, 26 Feb 2014 10:57:32 +0000 9, 29 -- Message-ID: {6936FD3A0444524A8FA0A7FB2BBAFDB40C3EBCCA-at-FLI-JE-MB031.intern.fli.bund.local} 9, 29 -- Accept-Language: de-DE, en-US 9, 29 -- Content-Language: de-DE 9, 29 -- X-MS-Has-Attach: 9, 29 -- X-MS-TNEF-Correlator: 9, 29 -- x-originating-ip: [172.18.2.80] 9, 29 -- Content-Type: text/plain; charset="iso-8859-1" 9, 29 -- MIME-Version: 1.0 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1QAvYMK027973 ==============================End of - Headers==============================
Dear Elisabeth, I found some old diskettes with following ATI MACH-64 drivers for Win3.11:
ATI Mach-64_2012 ATI Mach-64_222 ATI Mach-64_303
If you like, I can send you an archiv (zip) with all the files I have found on them.
Best regards from Prague
Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Wed, 26 Feb 2014 05:02:18 -0600, Elisabeth.Liebler-Tenorio-at-fli.bund.de wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear members of the listserver: } } We have a very old SEM XL20 (FEI, runs under Windows 3.11, engl. } version). Our hard disk crashed and we don't have a backup. } } We have replaced the hard disk and got MS DOS and Win 3.11. } installed, but we have problems with the operating system for the } SEM. FEI is not able to help us, because the XL20 is so old. } } Thus we are looking for: } - a backup of the XL20 operating system or } -Â XL20 - software V 5.24 (on discs or CD-ROM) including driver } software or } -Â EZ-SCSI-Driver (v3). (ADAPTEC-SCSI-SOFTWARE) or } -Â MACH 64- Driver or } -Â ATI-Driver for Win 3.11 (engl. Version). } } Sincerely, Elisabeth Liebler-Tenorio } } } } } } ==============================Original } Headers============================== 9, 29 -- From } Elisabeth.Liebler-Tenorio-at-fli.bund.de Wed Feb 26 04:57:35 2014 9, 29 } -- Received: from mx022.fli.bund.de (mx022.fli.bund.de } [193.22.112.22]) 9, 29 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id s1QAvYMK027973 9, 29 -- } for {Microscopy-at-Microscopy.Com} ; Wed, 26 Feb 2014 04:57:34 } -0600 9, 29 -- Received: from FLI-RIE-HT039.intern.fli.bund.local } (Not Verified[172.20.0.39]) by mx022.fli.bund.de with MailMarshal } (v7,2,2,6606) 9, 29 -- id {B530dc8900000} ; Wed, 26 Feb 2014 } 11:57:20 +0100 9, 29 -- Received: from } FLI-JE-MB031.intern.fli.bund.local (172.18.0.31) by 9, 29 -- } FLI-RIE-HT039.intern.fli.bund.local (172.20.0.39) with Microsoft SMTP } Server 9, 29 -- (TLS) id 14.3.174.1; Wed, 26 Feb 2014 11:57:34 +0100 } 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local } ([fe80::9167:29a:d9b4:9c20]) 9, 29 -- by } FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20%10]) } with 9, 29 -- mapi id 14.03.0174.001; Wed, 26 Feb 2014 11:57:33 } +0100 9, 29 -- From: "Liebler-Tenorio, Elisabeth" } {Elisabeth.Liebler-Tenorio-at-fli.bund.de} 9, 29 -- To: } "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 9, 29 -- CC: } "ethalmann-at-t-online.de" {ethalmann-at-t-online.de} 9, 29 -- Subject: SEM } XL20 (FEI) operating system/drivers 9, 29 -- Thread-Topic: SEM XL20 } (FEI) operating system/drivers 9, 29 -- Thread-Index: } Ac8y4WJQgviqvi0tQz6N3f8e25e20g== 9, 29 -- Date: Wed, 26 Feb 2014 } 10:57:32 +0000 9, 29 -- Message-ID: } {6936FD3A0444524A8FA0A7FB2BBAFDB40C3EBCCA-at-FLI-JE-MB031.intern.fli.bund.local} } 9, 29 -- Accept-Language: de-DE, en-US 9, 29 -- Content-Language: } de-DE 9, 29 -- X-MS-Has-Attach: 9, 29 -- X-MS-TNEF-Correlator: 9, 29 } -- x-originating-ip: [172.18.2.80] 9, 29 -- Content-Type: text/plain; } charset="iso-8859-1" 9, 29 -- MIME-Version: 1.0 9, 29 -- } Content-Transfer-Encoding: 8bit 9, 29 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id s1QAvYMK027973 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 40 -- From benada-at-biomed.cas.cz Wed Feb 26 08:09:01 2014 8, 40 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QE90Ln019616 8, 40 -- for {Microscopy-at-Microscopy.com} ; Wed, 26 Feb 2014 08:09:01 -0600 8, 40 -- X-ASG-Debug-ID: 1393423738-05011e721bd0290001-FOsErg 8, 40 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id SlsMxgyA6FQszjI5 (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO); Wed, 26 Feb 2014 15:08:58 +0100 (CET) 8, 40 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 8, 40 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 8, 40 -- Received: from u117fs (c111kh.mbu.cas.cz [147.231.44.14]) 8, 40 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 8, 40 -- (No client certificate requested) 8, 40 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 54B132C81A0; 8, 40 -- Wed, 26 Feb 2014 15:08:58 +0100 (CET) 8, 40 -- Date: Wed, 26 Feb 2014 15:08:56 +0100 8, 40 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 8, 40 -- To: Elisabeth.Liebler-Tenorio-at-fli.bund.de, Microscopy-at-Microscopy.com 8, 40 -- Subject: Re: [Microscopy] SEM XL20 (FEI) operating system/drivers 8, 40 -- Message-ID: {20140226150856.562b7ae3-at-u117fs} 8, 40 -- X-ASG-Orig-Subj: Re: [Microscopy] SEM XL20 (FEI) operating system/drivers 8, 40 -- In-Reply-To: {201402261102.s1QB2I7a030920-at-ns.microscopy.com} 8, 40 -- References: {201402261102.s1QB2I7a030920-at-ns.microscopy.com} 8, 40 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 8, 40 -- =?UTF-8?B?xIxS?= 8, 40 -- X-Mailer: Claws Mail 3.9.3 (GTK+ 2.24.10; i486-pc-linux-gnu) 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Type: text/plain; charset=UTF-8 8, 40 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 8, 40 -- X-Barracuda-Start-Time: 1393423738 8, 40 -- X-Barracuda-Encrypted: AES256-SHA 8, 40 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 8, 40 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 8, 40 -- X-Barracuda-BRTS-Status: 1 8, 40 -- X-Barracuda-Spam-Score: 0.00 8, 40 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 8, 40 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.145532 8, 40 -- Rule breakdown below 8, 40 -- pts rule name description 8, 40 -- ---- ---------------------- -------------------------------------------------- 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1QE90Ln019616 ==============================End of - Headers==============================
Last summer I had fun recovering WIN-NT PC on FEI FIB620 (Phillips XL30 SEM with ion beam column added) from a hard disk crash.
If your original disk is physically operational, then recovering SEM software from it may still be possible. Not sure if PC "hackery" is of interest for everyone, so please contact me off list you want/need to give it a try.
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/26/2014 5:59 AM, Elisabeth.Liebler-Tenorio-at-fli.bund.de wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear members of the listserver: } } We have a very old SEM XL20 (FEI, runs under Windows 3.11, engl. version). Our hard disk crashed and we don't have a backup. } } We have replaced the hard disk and got MS DOS and Win 3.11. installed, but we have problems with the operating system for the SEM. FEI is not able to help us, because the XL20 is so old. } } Thus we are looking for: } - a backup of the XL20 operating system or } - XL20 - software V 5.24 (on discs or CD-ROM) including driver software or } - EZ-SCSI-Driver (v3). (ADAPTEC-SCSI-SOFTWARE) or } - MACH 64- Driver or } - ATI-Driver for Win 3.11 (engl. Version). } } Sincerely, Elisabeth Liebler-Tenorio } } } } } } ==============================Original Headers============================== } 9, 29 -- From Elisabeth.Liebler-Tenorio-at-fli.bund.de Wed Feb 26 04:57:35 2014 } 9, 29 -- Received: from mx022.fli.bund.de (mx022.fli.bund.de [193.22.112.22]) } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QAvYMK027973 } 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Feb 2014 04:57:34 -0600 } 9, 29 -- Received: from FLI-RIE-HT039.intern.fli.bund.local (Not Verified[172.20.0.39]) by mx022.fli.bund.de with MailMarshal (v7,2,2,6606) } 9, 29 -- id {B530dc8900000} ; Wed, 26 Feb 2014 11:57:20 +0100 } 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local (172.18.0.31) by } 9, 29 -- FLI-RIE-HT039.intern.fli.bund.local (172.20.0.39) with Microsoft SMTP Server } 9, 29 -- (TLS) id 14.3.174.1; Wed, 26 Feb 2014 11:57:34 +0100 } 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20]) } 9, 29 -- by FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20%10]) with } 9, 29 -- mapi id 14.03.0174.001; Wed, 26 Feb 2014 11:57:33 +0100 } 9, 29 -- From: "Liebler-Tenorio, Elisabeth" {Elisabeth.Liebler-Tenorio-at-fli.bund.de} } 9, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 9, 29 -- CC: "ethalmann-at-t-online.de" {ethalmann-at-t-online.de} } 9, 29 -- Subject: SEM XL20 (FEI) operating system/drivers } 9, 29 -- Thread-Topic: SEM XL20 (FEI) operating system/drivers } 9, 29 -- Thread-Index: Ac8y4WJQgviqvi0tQz6N3f8e25e20g== } 9, 29 -- Date: Wed, 26 Feb 2014 10:57:32 +0000 } 9, 29 -- Message-ID: {6936FD3A0444524A8FA0A7FB2BBAFDB40C3EBCCA-at-FLI-JE-MB031.intern.fli.bund.local} } 9, 29 -- Accept-Language: de-DE, en-US } 9, 29 -- Content-Language: de-DE } 9, 29 -- X-MS-Has-Attach: } 9, 29 -- X-MS-TNEF-Correlator: } 9, 29 -- x-originating-ip: [172.18.2.80] } 9, 29 -- Content-Type: text/plain; charset="iso-8859-1" } 9, 29 -- MIME-Version: 1.0 } 9, 29 -- Content-Transfer-Encoding: 8bit } 9, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1QAvYMK027973 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 43 -- From vray-at-partbeamsystech.com Wed Feb 26 08:23:26 2014 5, 43 -- Received: from nm16-vm6.bullet.mail.ne1.yahoo.com (nm16-vm6.bullet.mail.ne1.yahoo.com [98.138.91.109]) 5, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QENPpK003558 5, 43 -- for {microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 08:23:25 -0600 5, 43 -- Received: from [98.138.101.130] by nm16.bullet.mail.ne1.yahoo.com with NNFMP; 26 Feb 2014 14:23:25 -0000 5, 43 -- Received: from [98.138.84.45] by tm18.bullet.mail.ne1.yahoo.com with NNFMP; 26 Feb 2014 14:23:25 -0000 5, 43 -- Received: from [127.0.0.1] by smtp113.mail.ne1.yahoo.com with NNFMP; 26 Feb 2014 14:23:25 -0000 5, 43 -- X-Yahoo-Newman-Id: 218200.74829.bm-at-smtp113.mail.ne1.yahoo.com 5, 43 -- X-Yahoo-Newman-Property: ymail-3 5, 43 -- X-YMail-OSG: NHu4eV8VM1l6KHm.QA.gbcMhOPEW.KAbNNGAIV_gcUOeBxM 5, 43 -- xmkAgqs8uy8nhJ8asaWu4V54p6Bzi1LyhZlC.E5DXfyD4TYn56kYvvYZa7_w 5, 43 -- FIfeaieqFCuSG6Rbc0O5NOdwyCKzeMVtH9TqN7Hz5WK7Z6w6pXrSV1LWAvrY 5, 43 -- L4PktQFlYXq2_OMevjr3s3A_Nv.7UX6qfro_j_A44iBPsXxKEYiWeDdPLQiK 5, 43 -- ep7gRgeAQGa2whGjs_mOrFyrvYt_LJlwm3oGpxOjm_QQHC3Ox9He5xvAjpfT 5, 43 -- PLqQ4Rsau6ZXlc3Imra3HNwGgdMcYiDvBucCC3oclwcOqMkqpuyL4EXp5wVF 5, 43 -- NZdmCPhFg8OW5kcivz6YFcivvfPBDgqy3_Xdtt0Nvpz7QafSmPVyIClbp.EV 5, 43 -- 26.Ccgw8giBEt79_ydhOk3fbhPbNVqpOoQ_L5R4dIIVLzqi_74QfnCKYGwvz 5, 43 -- 4JHd8cG3928ULJfWvyosDJOgEiLLmGNV43zafiwVKjY7PzU1ICQgbXyOsOw9 5, 43 -- mC5gKRHCWEE.M3Xx8RBq7LMMQaVbH0MzE_1UFudEmYF4ZdLYxf6x4ZTQR4im 5, 43 -- ceFmmYoDb7qX2V.YWYQK42DaVQKwr.v3OrS3UK0mWbw7KYP9QZfShz7nG8qf 5, 43 -- 5oYefWkxVN2SAfER._eY.Ad8j63uhQP3gKD2wCC81GJCERc6fL9HwHognAkS 5, 43 -- 0ZjHMsAGCk_oCf92vpzo7qr6aTZtmAcdlosXG3jh0_YwhC2Srsa4XxJkxmad 5, 43 -- Zjrk2_i1VJrk5Lx4dMgIllyIUCd7_qfOMAHMMtuv0VJ2PBKPuKAQzkfTwS8T 5, 43 -- actcyPOTn2wvtPgh6O.FXRl4s.CQxHtxjVWB43WQ8_.epMaJl_pkQ9tx3yvE 5, 43 -- BLHuwE4Vnc5aqEDPl1FAnkRLdw7XC8Dn9lBdZ77ALBQZ08vwoykrcQ3p7CZn 5, 43 -- SNi6BH8TrhDg- 5, 43 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 43 -- X-Rocket-Received: from [192.168.15.134] (vray-at-75.93.147.105 with plain [98.139.211.125]) 5, 43 -- by smtp113.mail.ne1.yahoo.com with SMTP; 26 Feb 2014 06:23:25 -0800 PST 5, 43 -- Message-ID: {530DF8DB.6050206-at-partbeamsystech.com} 5, 43 -- Date: Wed, 26 Feb 2014 09:23:23 -0500 5, 43 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 5, 43 -- Reply-To: vray-at-partbeamsystech.com 5, 43 -- Organization: PBS&T 5, 43 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 5, 43 -- MIME-Version: 1.0 5, 43 -- To: Elisabeth.Liebler-Tenorio-at-fli.bund.de 5, 43 -- CC: microscopy-at-microscopy.com 5, 43 -- Subject: Re: [Microscopy] SEM XL20 (FEI) operating system/drivers 5, 43 -- References: {201402261059.s1QAx9uw029163-at-ns.microscopy.com} 5, 43 -- In-Reply-To: {201402261059.s1QAx9uw029163-at-ns.microscopy.com} 5, 43 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 43 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A user of our JEOL 2100 TEM informed me that he was noticing anomalous rings appearing in diffraction patterns acquired with the SAD aperture centered over vacuum (he's developing a quantitative diffraction technique and presumably is acquiring a background diffraction spectrum). I triple checked the microscope alignment in TEM mode, STEM, nanobeam diffraction, convergent beam diffraction, and the high current EDS mode are all well aligned over the full range of spot sizes and convergence angles. The lens voltages and alignment coils all fall within their suitable ranges as well. When I acquired diffraction patterns of actual sample I don't see the artifacts in the DP, presumably because the intensity of the actual diffraction rings/spots washes out the weak artifact rings.
I checked all the condensor and selected area apertures, and all exhibit the problem as far as I can tell. I also triple checked to ensure all other apertures were removed and not displaced into a wrong position by a user, and they all checked out as well.
I'm attaching a link to two images of the artifact. These were taken at 200kV but the same is seen at 120kV (and probably 80kV--have not checked). You can see that there are two rings and their size and spacing change with changing camera lengths. Is this caused by diffraction of something in the beam path--either an aperture carrier or lost sample--or another effect, or am I missing something simple? Thanks for any hints or help directed my way.
Link to DPs showing artifacts: http://imgur.com/a/xCV4R
Thanks, Chris Virginia Tech
==============================Original Headers============================== 6, 26 -- From microwink-at-gmail.com Wed Feb 26 17:23:29 2014 6, 26 -- Received: from mail-yh0-f44.google.com (mail-yh0-f44.google.com [209.85.213.44]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QNNSl2004342 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 17:23:28 -0600 6, 26 -- Received: by mail-yh0-f44.google.com with SMTP id f73so1872230yha.17 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 15:23:28 -0800 (PST) 6, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=20120113; 6, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 6, 26 -- bh=YnUClmt1SX7+iNo6a5C5nRvjVCMrdlTnLTwfTKWxnMI=; 6, 26 -- b=UWHMLVjuyASU2VIeWj9DTIqV7UpS3YgnVDMF5b3MM3UaWdNstB6mGRufrEin2TO56S 6, 26 -- cp6qZ0bQKggJ1XvulYzyMxOg9tjsxPWWqsmxqP3f2WdasGp8vltqHmH4E70elKoSFuzW 6, 26 -- J7gyq8tUTk0NzuJh86xgG819RMXRdWPdyYr8DbCoDv655Nz5tSAIBiu/XOOdLerV+4Iw 6, 26 -- FzdhgzNu6euCnn4Ly2hj+mmJBPm32U33lZE5DN9OPOQnRvtyhQF32o9CyCsa+osenzsc 6, 26 -- yWmX/XucvhB2Z2ZbY5mLUXkLCEGQOR6oFR6XkYFKG+V1yZHQUgInl77IsKfQVkzl/QC0 6, 26 -- CvNA== 6, 26 -- MIME-Version: 1.0 6, 26 -- X-Received: by 10.236.152.36 with SMTP id c24mr5737180yhk.118.1393457008189; 6, 26 -- Wed, 26 Feb 2014 15:23:28 -0800 (PST) 6, 26 -- Received: by 10.170.180.8 with HTTP; Wed, 26 Feb 2014 15:23:28 -0800 (PST) 6, 26 -- Date: Wed, 26 Feb 2014 18:23:28 -0500 6, 26 -- Message-ID: {CAA8T2PO4yOMNE7O7XY=H-AzOvCq3H7pAQNOE=oUq4F_0TvrGRg-at-mail.gmail.com} 6, 26 -- Subject: Anomalous diffraction rings in JEOL 2100 6, 26 -- From: Christopher Winkler {microwink-at-gmail.com} 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
On Feb 26, 2014, at 3:34 PM, microwink-at-gmail.com wrote:
} A user of our JEOL 2100 TEM informed me that he was noticing anomalous } rings appearing in diffraction patterns acquired with the SAD aperture } centered over vacuum (he's developing a quantitative diffraction } technique and presumably is acquiring a background diffraction } spectrum). I triple checked the microscope alignment in TEM mode, } STEM, nanobeam diffraction, convergent beam diffraction, and the high } current EDS mode are all well aligned over the full range of spot } sizes and convergence angles. The lens voltages and alignment coils } all fall within their suitable ranges as well. When I acquired } diffraction patterns of actual sample I don't see the artifacts in the } DP, presumably because the intensity of the actual diffraction } rings/spots washes out the weak artifact rings. } } I checked all the condensor and selected area apertures, and all } exhibit the problem as far as I can tell. I also triple checked to } ensure all other apertures were removed and not displaced into a wrong } position by a user, and they all checked out as well. } } I'm attaching a link to two images of the artifact. These were taken } at 200kV but the same is seen at 120kV (and probably 80kV--have not } checked). You can see that there are two rings and their size and } spacing change with changing camera lengths. Is this caused by } diffraction of something in the beam path--either an aperture carrier } or lost sample--or another effect, or am I missing something simple? } Thanks for any hints or help directed my way. } } Link to DPs showing artifacts: http://imgur.com/a/xCV4R } } Thanks, } Chris } Virginia Tech } Dear Chris, I noticed that the rings are not centered with the undiffracted beam. Also, the pattern appears to be from a polycrystalline specimen. It may be that the beam emitted by the FEG/filament is split in two--either by having two points emitting from the source or by the beam hitting something near the top of the column producing a scattered beam. If this second beam strikes some polycrystalline substance, such as a grid near the point of vacuum where the main beam is directed, it could produce something like the DPs on the web site. Do the DPs change when the stage is moved (while keeping the beam over vacuum, of course)? Yours, Bill
==============================Original Headers============================== 6, 37 -- From wtivol-at-sbcglobal.net Wed Feb 26 20:49:06 2014 6, 37 -- Received: from nm4.access.bullet.mail.gq1.yahoo.com (nm4.access.bullet.mail.gq1.yahoo.com [216.39.62.35]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1R2n5rD026815 6, 37 -- for {microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 20:49:06 -0600 6, 37 -- Received: from [216.39.60.165] by nm4.access.bullet.mail.gq1.yahoo.com with NNFMP; 27 Feb 2014 02:49:05 -0000 6, 37 -- Received: from [67.195.23.146] by tm1.access.bullet.mail.gq1.yahoo.com with NNFMP; 27 Feb 2014 02:49:05 -0000 6, 37 -- Received: from [127.0.0.1] by smtp118.sbc.mail.gq1.yahoo.com with NNFMP; 27 Feb 2014 02:49:05 -0000 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s1024; t=1393469345; bh=NAfGz1+z1PWb307Um5ez/cLfUfFZ7ws6BHtFsoWd50A=; h=X-Yahoo-Newman-Id:X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:Cc:Message-Id:From:To:In-Reply-To:Content-Type:Content-Transfer-Encoding:Mime-Version:Subject:Date:References:X-Mailer; b=RWn/yyOh6s8T0ptuPxh1nK5RzKl3XIphkJv0cKEgPwOHDUoueT7i9+lPYDh/0qjaWmxyapMNSdKbQY3HavPLSYW6Twk9cb8VnwS1AuAMZ4rJ8VJlERUzRiVzvWBYqG/n420yoH0dUveskPTkBmTcxz1H0SoYTTSI+WbnCKiGYIs= 6, 37 -- X-Yahoo-Newman-Id: 53491.57855.bm-at-smtp118.sbc.mail.gq1.yahoo.com 6, 37 -- X-Yahoo-Newman-Property: ymail-3 6, 37 -- X-YMail-OSG: mi394ygVM1mlzvwPWYKq5q.g5AzZ6Cqyu_R_CgGDJ.kLL4q 6, 37 -- bKG0rR83LENITjBnNpkqj8YOT8Kp.b3H4yrAv9DQPGhmnnCrSXCTDsJmeZbb 6, 37 -- cxxu40qv9KV7r1d1esr_n8PNOU_9xJLmtpr09NuuXNjp2uKaWwSb7Yg6mK_B 6, 37 -- bugBTvx.qUWBUYBLiFYKZVjvTlKzwg1bYxrxbuo4KCDH3ubyKfgpLLHxgavf 6, 37 -- bnvRLsHkwwGVphK51llvC2v8hyT1wAWZ6d74VKD7drLtDAcKOzxfYaKZQ.QR 6, 37 -- pkPjvYt1NefwdaUrj3tTLr3k.KN4pGV376jt3.JPaNhkkJ1.Zx6UycHa8Vl5 6, 37 -- RJj.iG4JwdVxeLDDqmrK1xqoBqX88AGlk83DxXnfAkGBbEzNCGsqcDHo2AFP 6, 37 -- l5ux1lp_aAC0du1XyAyp.SIEru4tIk.1jRRjc7U4VrgB9Ij_DdphYUF.6JrL 6, 37 -- _ijfERFssFZ8z7PveK8LtyyHdDylFvrvywsdCniw6oIIWZsp95juP4XCzBBx 6, 37 -- 5S2D1oyBzrdx2gVUh3jCaLpprw.yLQ_QrFrky8wU9kVCwJPFRoicde2uviIY 6, 37 -- C13KK1bJK5C8NXtt8l38sGAuZ33h6otiu7QFBT2lq68kEKNEuTNrCR.lYvPf 6, 37 -- 2pNcguzOgqeXLqA-- 6, 37 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 37 -- X-Rocket-Received: from [192.168.1.68] (wtivol-at-108.226.244.17 with plain [67.195.15.66]) 6, 37 -- by smtp118.sbc.mail.gq1.yahoo.com with SMTP; 27 Feb 2014 02:49:05 +0000 UTC 6, 37 -- Cc: microscopy-at-microscopy.com 6, 37 -- Message-Id: {77CAAC6F-3B04-4011-9597-B2C9840AC9C2-at-sbcglobal.net} 6, 37 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 37 -- To: microwink-at-gmail.com 6, 37 -- In-Reply-To: {201402262334.s1QNYjkh019441-at-ns.microscopy.com} 6, 37 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 37 -- Content-Transfer-Encoding: 7bit 6, 37 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 37 -- Subject: Re: [Microscopy] Anomalous diffraction rings in JEOL 2100 6, 37 -- Date: Wed, 26 Feb 2014 18:49:03 -0800 6, 37 -- References: {201402262334.s1QNYjkh019441-at-ns.microscopy.com} 6, 37 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Yes, I used to see these on our JEOL 2500 when I was at HP. Tomba San, Mr Tomba at JEOL fixed it by always having us do diffraction left of crossover- where the beam is more parallel! That will most likely give the heave ho to your rings!
Ganbette Good Luck or Hang in there!
Pete Eschbach Oregon State EM Facility
Sent from my iPhone
} On Feb 26, 2014, at 3:35 PM, microwink-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all, } } A user of our JEOL 2100 TEM informed me that he was noticing anomalous } rings appearing in diffraction patterns acquired with the SAD aperture } centered over vacuum (he's developing a quantitative diffraction } technique and presumably is acquiring a background diffraction } spectrum). I triple checked the microscope alignment in TEM mode, } STEM, nanobeam diffraction, convergent beam diffraction, and the high } current EDS mode are all well aligned over the full range of spot } sizes and convergence angles. The lens voltages and alignment coils } all fall within their suitable ranges as well. When I acquired } diffraction patterns of actual sample I don't see the artifacts in the } DP, presumably because the intensity of the actual diffraction } rings/spots washes out the weak artifact rings. } } I checked all the condensor and selected area apertures, and all } exhibit the problem as far as I can tell. I also triple checked to } ensure all other apertures were removed and not displaced into a wrong } position by a user, and they all checked out as well. } } I'm attaching a link to two images of the artifact. These were taken } at 200kV but the same is seen at 120kV (and probably 80kV--have not } checked). You can see that there are two rings and their size and } spacing change with changing camera lengths. Is this caused by } diffraction of something in the beam path--either an aperture carrier } or lost sample--or another effect, or am I missing something simple? } Thanks for any hints or help directed my way. } } Link to DPs showing artifacts: http://imgur.com/a/xCV4R } } Thanks, } Chris } Virginia Tech } } ==============================Original Headers============================== } 6, 26 -- From microwink-at-gmail.com Wed Feb 26 17:23:29 2014 } 6, 26 -- Received: from mail-yh0-f44.google.com (mail-yh0-f44.google.com [209.85.213.44]) } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QNNSl2004342 } 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 17:23:28 -0600 } 6, 26 -- Received: by mail-yh0-f44.google.com with SMTP id f73so1872230yha.17 } 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 15:23:28 -0800 (PST) } 6, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 26 -- d=gmail.com; s=20120113; } 6, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; } 6, 26 -- bh=YnUClmt1SX7+iNo6a5C5nRvjVCMrdlTnLTwfTKWxnMI=; } 6, 26 -- b=UWHMLVjuyASU2VIeWj9DTIqV7UpS3YgnVDMF5b3MM3UaWdNstB6mGRufrEin2TO56S } 6, 26 -- cp6qZ0bQKggJ1XvulYzyMxOg9tjsxPWWqsmxqP3f2WdasGp8vltqHmH4E70elKoSFuzW } 6, 26 -- J7gyq8tUTk0NzuJh86xgG819RMXRdWPdyYr8DbCoDv655Nz5tSAIBiu/XOOdLerV+4Iw } 6, 26 -- FzdhgzNu6euCnn4Ly2hj+mmJBPm32U33lZE5DN9OPOQnRvtyhQF32o9CyCsa+osenzsc } 6, 26 -- yWmX/XucvhB2Z2ZbY5mLUXkLCEGQOR6oFR6XkYFKG+V1yZHQUgInl77IsKfQVkzl/QC0 } 6, 26 -- CvNA== } 6, 26 -- MIME-Version: 1.0 } 6, 26 -- X-Received: by 10.236.152.36 with SMTP id c24mr5737180yhk.118.1393457008189; } 6, 26 -- Wed, 26 Feb 2014 15:23:28 -0800 (PST) } 6, 26 -- Received: by 10.170.180.8 with HTTP; Wed, 26 Feb 2014 15:23:28 -0800 (PST) } 6, 26 -- Date: Wed, 26 Feb 2014 18:23:28 -0500 } 6, 26 -- Message-ID: {CAA8T2PO4yOMNE7O7XY=H-AzOvCq3H7pAQNOE=oUq4F_0TvrGRg-at-mail.gmail.com} } 6, 26 -- Subject: Anomalous diffraction rings in JEOL 2100 } 6, 26 -- From: Christopher Winkler {microwink-at-gmail.com} } 6, 26 -- To: Microscopy-at-microscopy.com } 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 35 -- From peter.eschbach-at-comcast.net Thu Feb 27 11:07:48 2014 7, 35 -- Received: from qmta08.emeryville.ca.mail.comcast.net (qmta08.emeryville.ca.mail.comcast.net [76.96.30.80]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1RH7lRS029103 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 27 Feb 2014 11:07:48 -0600 7, 35 -- Received: from omta23.emeryville.ca.mail.comcast.net ([76.96.30.90]) 7, 35 -- by qmta08.emeryville.ca.mail.comcast.net with comcast 7, 35 -- id XUNS1n0021wfjNsA8V7nsn; Thu, 27 Feb 2014 17:07:47 +0000 7, 35 -- Received: from [IPv6:2601:7:1400:944:d00a:c8f2:f8db:188a] ([IPv6:2601:7:1400:944:d00a:c8f2:f8db:188a]) 7, 35 -- by omta23.emeryville.ca.mail.comcast.net with comcast 7, 35 -- id XV7m1n00J3S7LFo8jV7mj1; Thu, 27 Feb 2014 17:07:47 +0000 7, 35 -- Subject: Re: [Microscopy] Anomalous diffraction rings in JEOL 2100 7, 35 -- References: {201402262335.s1QNZFrh020221-at-ns.microscopy.com} 7, 35 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 7, 35 -- Content-Type: text/plain; 7, 35 -- charset=us-ascii 7, 35 -- X-Mailer: iPhone Mail (11B554a) 7, 35 -- In-Reply-To: {201402262335.s1QNZFrh020221-at-ns.microscopy.com} 7, 35 -- Message-Id: {E1A91431-90BC-4DBD-899C-62A1F838DA57-at-comcast.net} 7, 35 -- Date: Thu, 27 Feb 2014 09:07:47 -0800 7, 35 -- To: "microwink-at-gmail.com" {microwink-at-gmail.com} , 7, 35 -- "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 7, 35 -- Mime-Version: 1.0 (1.0) 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 7, 35 -- s=q20121106; t=1393520867; 7, 35 -- bh=CnWCEsaPwyXIAT4u9IhI5qHRfFttXjLbgwhlKMuumXI=; 7, 35 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: 7, 35 -- Mime-Version; 7, 35 -- b=NSFq4OMr77tuu9gqm9RByys3+fUESSO77yDBaf5u09++SR3j9ZQWh+ib/wUqsSenE 7, 35 -- vUhWEjsp9JaRQgeFgM105zZ27tqn+TZz8fBW7F9mgmAIS8i55ef57AhDeIcj8vkVBe 7, 35 -- nnXm61KRqQ50vDqylI1LU/+RXN9yJZKLlUQuv7nyBbh0mY3EbT+qzloB07PLtF6Bg0 7, 35 -- D8hFh9fD0JoUBGq0xjeV+R+tZNcghIeViB/5Iewp/8aD2hhtpoUUZOEjEFP9l7qNiz 7, 35 -- Ed4j8TjP7Nu56EETAYqVpne0vbzMCscgs6Tcr8teN4G3XgqZZ1ZCngPVCT6KNiClgM 7, 35 -- 0uLFrIcV2HFkw== 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1RH7lRS029103 ==============================End of - Headers==============================
The deadline for abstract submission has passed and as you are making plans to attend the Hartford meetings Aug. 3-7, please don*t forget about MSA*s student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs; giving them an opportunity to meet and interact with the established microscopy community.
The students will be paid $10 an hour to work for ~20 hours (up to 40 hours) during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia (assisting with audio-visual needs, speaker set-up, maintaining an attendance count), staffing the volunteer office, monitoring use of the Internet Café, and helping with vendor tutorial sign-up. Payment is given as a check at the end of the meetings or when the student leaves Hartford.
Once the program has been finalized, each registered bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up *working* sessions they would attend anyway. There is an added bonus of $10 cash to help with meals for each morning and/or afternoon session worked and a meeting shirt.
If anyone would like to participate in the bursary program, please check the *I wish to apply for a student bursary* box in section 2 of the registration form and send me an email of your intention. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.
For those *non-students* volunteers are also needed to help with the above mentioned meeting activities. Although not paid on an hourly basis as the student bursaries, volunteers do receive a meeting shirt and the same cash allotment to help with meals. Volunteers also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact:
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-7998 alawrence-at-i2at.msstate.edu
==============================Original Headers============================== 11, 23 -- From ALawrence-at-i2at.msstate.edu Thu Feb 27 14:28:21 2014 11, 23 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1RKSLes019434 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 27 Feb 2014 14:28:21 -0600 11, 23 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197]) 11, 23 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id s1RKSLSn018820 11, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 27 Feb 2014 14:28:21 -0600 11, 23 -- X-Sender: {} 11, 23 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu 11, 23 -- with Novell_GroupWise; Thu, 27 Feb 2014 14:28:21 -0600 11, 23 -- Message-Id: {530F4B83020000E6000DA098-at-mailhost.groupwise.msstate.edu} 11, 23 -- X-Mailer: Novell GroupWise Internet Agent 12.0.2 11, 23 -- Date: Thu, 27 Feb 2014 14:28:19 -0600 11, 23 -- From: "Amanda Lawrence" {ALawrence-at-i2at.msstate.edu} 11, 23 -- To: {microscopy-at-microscopy.com} 11, 23 -- Subject: M&M 2014 Student Bursaries and Volunteers 11, 23 -- References: {530F3EE6020000E6000DA088-at-mailhost.groupwise.msstate.edu} 11, 23 -- {530F4B83020000E6000DA098-at-mailhost.groupwise.msstate.edu} 11, 23 -- Mime-Version: 1.0 11, 23 -- Content-Type: text/plain; charset=ISO-8859-15 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- Content-Disposition: inline ==============================End of - Headers==============================
X-from: lamiller-at-illinois.edu () To: Zaluzec-at-MICROSCOPY.COM
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Email: lamiller-at-illinois.edu Name: Lou Ann Miller
Organization: Materials Research Lab, U of Illinois
Title-Subject: [Filtered] MRL Biological microscopy and analysis work shop Opportunity for vendors, and for students
Message: Hello!
Many thanks to many of you for helping out with our fall biological conference in 2013!
This year, we hope to have the conference November 12 & 13th, 2014.
Students: There will be a poster and presentation Competition at this year's MRL Biological microscopy and analysis work shop.
Each year we have had a different theme, 1st Biostructures and "what are we looking at", last year Techniques: " how do we do that?"
This year, I'm hoping to pull in more students and participation by having a student presentation and poster competition as well as talks by those who have specialties here on campus.. The subject will be how students put biological microscopy techniques into use.
VENDORS:
We would like to offer prizes for the best presentations with posters. Of course this is where you can come in. :-)
Because you are vendors with the university, we checked all the rules, and it appears that a check from a vendor straight to the prize winner can be up to , but not exceed $100.
The first 3 vendors, who can offer a nice monetary prize($100 or less), will get to be on the judges panel, as well as have the prize named after your company. We can send the results with images with your company's name to our paper, and to the university paper.
Vendors, please let me know by March 30th if you are interested.
If you miss this, and still would like mention around the conference, feel free to email me about sponsoring a break for food.
Students, more information will be forthcoming for you. With biological applications also mixing with material science these days, you may be interested if you mix the two disciplines as well.
Thanks!
Lou Ann Miller Biology Specialist, Frederick Seitz Materials Research Lab
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==============================Original Headers============================== 34, 29 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 28 07:23:18 2014 34, 29 -- Received: from znl.com ([206.69.208.20]) 34, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SDNIrK030295 34, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:18 -0600 34, 29 -- Received: from localhost (localhost [127.0.0.1]) 34, 29 -- by znl.com (Postfix) with ESMTP id A66DF6C78FB 34, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:18 -0600 (CST) 34, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 34, 29 -- Received: from znl.com ([127.0.0.1]) 34, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 34, 29 -- with ESMTP id Gf7Sp8RveAVg for {microscopy-at-microscopy.com} ; 34, 29 -- Fri, 28 Feb 2014 07:23:11 -0600 (CST) 34, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 34, 29 -- by znl.com (Postfix) with ESMTPA id 1294C6C78E9 34, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:11 -0600 (CST) 34, 29 -- Message-ID: {53108DBE.4060201-at-microscopy.com} 34, 29 -- Date: Fri, 28 Feb 2014 07:23:10 -0600 34, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 34, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 34, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 34, 29 -- MIME-Version: 1.0 34, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 34, 29 -- Subject: viaWWW:UIUC MRL Biological microscopy and analysis work shop Opportunity 34, 29 -- for vendors, and for students 34, 29 -- References: {201402271524.s1RFO1os024645-at-ns.microscopy.com} 34, 29 -- In-Reply-To: {201402271524.s1RFO1os024645-at-ns.microscopy.com} 34, 29 -- X-Forwarded-Message-Id: {201402271524.s1RFO1os024645-at-ns.microscopy.com} 34, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 34, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ghoshroy-at-sc.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
We have Denton DESK II Turbo Sputter Coater and are planning to use a chromium sputtering target for the first time. We intend to use this coating for FESEM observation of a variety of specimens.We are familiar with gold and gold-palladium targets but never used a chromium target before. This morning we coated a sample with chromium, but seems like it didn't get any coating at all. I did see the plasma. When we use gold or gold-palladium in the same coater, it works fine and we can see the coating on the surface. Is it possible to see a color difference after Cr coating for 60-100 sec. We are using 40-60% power.I noticed the Cr target is much thicker than the gold target.
I will appreciate someone telling if we are doing something wrong or it is just our simple ignorance about chromium target. Any help will be very much appreciated.
Thanks in advance,
Soumitra
Soumitra Ghoshroy EM Center University of South Carolina Columbia, SC 29208
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 28 07:23:51 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SDNpnZ030881 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:51 -0600 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id BDC366C790A 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:51 -0600 (CST) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id Ef0vpv91LP0l for {microscopy-at-microscopy.com} ; 16, 28 -- Fri, 28 Feb 2014 07:23:48 -0600 (CST) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id D2D156C78FD 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:48 -0600 (CST) 16, 28 -- Message-ID: {53108DE4.3060705-at-microscopy.com} 16, 28 -- Date: Fri, 28 Feb 2014 07:23:48 -0600 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW: Chromium sputtering target 16, 28 -- References: {201402271927.s1RJR9XD018573-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201402271927.s1RJR9XD018573-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201402271927.s1RJR9XD018573-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: gcat84-at-gmail.com Name: Glenn
Organization: University of Delaware
Title-Subject: [Filtered] ISI DS-130 - schematics
Message: Hello,
I have an ISI DS-130 in mostly working condition. However, it recently gave up on producing some of the +/- 15 V outputs. The first indication was when the vacuum would kick out when enabling the filament. Then, the emission displays 200 uA even when the voltage and current are set to zero.
Does anyone have original wiring diagrams for this model SEM unit? It would be greatly appreciated.
Regards, Glenn
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 28 07:24:48 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SDOkDM031961 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:24:46 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 057CB6C7919 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:24:46 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id aFg3cd7MvLfV for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 28 Feb 2014 07:24:44 -0600 (CST) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 9017E6C790C 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:24:44 -0600 (CST) 14, 28 -- Message-ID: {53108E1C.3080309-at-microscopy.com} 14, 28 -- Date: Fri, 28 Feb 2014 07:24:44 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Needed ISI DS-130 - schematics 14, 28 -- References: {201402280350.s1S3o1TQ017503-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201402280350.s1S3o1TQ017503-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201402280350.s1S3o1TQ017503-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I do not know the Denton Turbo Sputter Coater, but I was involved with the development of Chromium coating many years ago!
To sputter chromium is not like sputtering any of the more familiar materials. Firstly, you need a high vacuum and a power supply that is rated much higher than for the conventional sputtering materials. Secondly, before you are able to sputter the chromium the oxide coating has to be removed, and this has to happen every time you use the unit. So the first part of the sputter process is oxide removal, most dedicated systems have a shutter to catch the oxide, thus leave the specimens clean. You know when all of the oxide has been removed as the plasma will have a nice pale blue colour. Once you have this level of target cleaning you switch off the plasma, remove the protective shutter, and coat for about 2 to 5 seconds. You should not expect to be able to see the coating.
It's a very long time since I was involved with this work but I hope I have been able to provide some assistance?
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
We have Denton DESK II Turbo Sputter Coater and are planning to use a chromium sputtering target for the first time. We intend to use this coating for FESEM observation of a variety of specimens.We are familiar with gold and gold-palladium targets but never used a chromium target before. This morning we coated a sample with chromium, but seems like it didn't get any coating at all. I did see the plasma. When we use gold or gold-palladium in the same coater, it works fine and we can see the coating on the surface. Is it possible to see a color difference after Cr coating for 60-100 sec. We are using 40-60% power.I noticed the Cr target is much thicker than the gold target.
I will appreciate someone telling if we are doing something wrong or it is just our simple ignorance about chromium target. Any help will be very much appreciated.
Thanks in advance,
Soumitra
Soumitra Ghoshroy EM Center University of South Carolina Columbia, SC 29208
==============================Original Headers============================== 20, 24 -- From protrain-at-emcourses.com Fri Feb 28 08:03:44 2014 20, 24 -- Received: from mail.esentra.net (mail.esentra.net [94.228.36.208]) 20, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SE3h0X023155 20, 24 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 08:03:44 -0600 20, 24 -- Received: from mail.clientmail.net (Not Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway 20, 24 -- id {B5310973e0000} ; Fri, 28 Feb 2014 14:03:42 +0000 20, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Fri, 28 Feb 2014 14:03:41 +0000 20, 24 -- Reply-To: {protrain-at-emcourses.com} 20, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} 20, 24 -- To: {ghoshroy-at-sc.edu} 20, 24 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 20, 24 -- References: {201402281325.s1SDPkKA001008-at-ns.microscopy.com} 20, 24 -- In-Reply-To: {201402281325.s1SDPkKA001008-at-ns.microscopy.com} 20, 24 -- Subject: RE: [Microscopy] viaWWW: Chromium sputtering target 20, 24 -- Date: Fri, 28 Feb 2014 14:03:41 -0000 20, 24 -- Organization: Protrain 20, 24 -- Message-ID: {001d01cf348d$e3309a00$a991ce00$-at-com} 20, 24 -- MIME-Version: 1.0 20, 24 -- Content-Type: text/plain; 20, 24 -- charset="us-ascii" 20, 24 -- Content-Transfer-Encoding: 7bit 20, 24 -- X-Mailer: Microsoft Office Outlook 12.0 20, 24 -- Thread-Index: Ac80iJZe8ZT+E30bQTOloqRWS7R+/AAA2JOg 20, 24 -- Content-Language: en-gb ==============================End of - Headers==============================
We are working with an ESEM Quanta 600 in the low vacuum mode. We need a pressure of 0.18 Torr, but when we choose it via the UI the pressure is not constant but jumps between ~0.17 and ~0.28 Torr. At our second ESEM Quanta 200 we experience no problems with the pressure stability at 0.18 Torr.
Do you have an idea, which parameter or defect is responsible?
Thank you in advance!
Stefan
==============================Original Headers============================== 6, 30 -- From stefan.wernitznig-at-medunigraz.at Fri Feb 28 08:36:34 2014 6, 30 -- Received: from si068.medunigraz.at (si173.medunigraz.at [193.170.105.173]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SEaXUn009951 6, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 08:36:33 -0600 6, 30 -- X-AuditID: 0ac80c44-f79eb6d000000e72-07-53109ef1f9a8 6, 30 -- Received: from sj063.medunigraz.at (Unknown_Domain [10.200.18.79]) 6, 30 -- by si068.medunigraz.at (Symantec Mail Security) with SMTP id B2.87.03698.1FE90135; Fri, 28 Feb 2014 15:36:33 +0100 (CET) 6, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at [10.200.112.128]) 6, 30 -- by sj063.medunigraz.at with ESMTP (TLS encrypted); Fri, 28 Feb 2014 15:36:27 +0100 6, 30 -- Message-ID: {53109EEF.8090506-at-medunigraz.at} 6, 30 -- Date: Fri, 28 Feb 2014 15:36:31 +0100 6, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} 6, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 6, 30 -- MIME-Version: 1.0 6, 30 -- To: Microscopy-at-microscopy.com 6, 30 -- Subject: Pressure stability problems in the low vacuum mode of an ESEM 6, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 6, 30 -- Content-Transfer-Encoding: 7bit 6, 30 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFlrDIsWRmVeSWpSXmKPExsXCdULIX/fjPIFgg6nTeCz+HG5gd2D0+Nj9 6, 30 -- mimAMYrLJiU1J7MstUjfLoEr487lB8wF85grdi9sYGtgPMzUxcjJISFgItG88xgLhC0mceHe 6, 30 -- erYuRi4OIYG9jBKr/h1lhnDmMUr8/TCTDaSKV0BHom3zTLAOFgFVifd321lBbDYBS4nj3T/A 6, 30 -- 4qICwRK7Hz9jhagXlDg58wlYXERAVmLB+yPsILawgJvEjb+fwK5gFrCR+HtiEQuELS+x/e0c 6, 30 -- 5gmMvLOQtM9CUjYLSdkCRuZVjMLFmQZmFnq5qSmleZnpRYlVeoklmxjBgcPjsoOxYYvhIUYB 6, 30 -- DkYlHl7pmQLBQqyJZcWVuYcYJTmYlER5788BCvEl5adUZiQWZ8QXleakFh9ilOBgVhLhfdIE 6, 30 -- lONNSaysSi3Kh0lJc7AoifN2hvMHCwmkJ5akZqemFqQWwWRlODiUJHifzAVqFCxKTU+tSMvM 6, 30 -- KUFIM3FwggznARpeArKYt7ggMbc4Mx0if4pRUkqcdx5IswBIIqM0D673FaM40AvCvO0gWR5g 6, 30 -- EoDregU0kAloIKcn2MCSRISUVAPjqZjlVVu4PGMfLLjTGnlrqQBb+sns1Brtts91338VWTre 6, 30 -- 1F2zam652PPeY/fXWEgmv9ubpnr15cz4+bmPlXpuPTv1cpf41zm+tSudry+bs/Vq0Nxo58um 6, 30 -- t3Rrcvczyp9qXCh46lzL2uXewokhVbsKGkRVOedfPzNvwu6blxKW7Vyxpn9G/X2xRCWW4oxE 6, 30 -- Qy3mouJEAFzE14i/AgAA ==============================End of - Headers==============================
Soumitra, as a compromise might I suggest using Pt, it will definitely give you better results than Au or Au/Pd.
Good Luck, John Robson
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Friday, February 28, 2014 9:13 AM To: Robson,John (AN) BIP-US-R
Hi
I do not know the Denton Turbo Sputter Coater, but I was involved with the development of Chromium coating many years ago!
To sputter chromium is not like sputtering any of the more familiar materials. Firstly, you need a high vacuum and a power supply that is rated much higher than for the conventional sputtering materials. Secondly, before you are able to sputter the chromium the oxide coating has to be removed, and this has to happen every time you use the unit. So the first part of the sputter process is oxide removal, most dedicated systems have a shutter to catch the oxide, thus leave the specimens clean. You know when all of the oxide has been removed as the plasma will have a nice pale blue colour. Once you have this level of target cleaning you switch off the plasma, remove the protective shutter, and coat for about 2 to 5 seconds. You should not expect to be able to see the coating.
It's a very long time since I was involved with this work but I hope I have been able to provide some assistance?
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
We have Denton DESK II Turbo Sputter Coater and are planning to use a chromium sputtering target for the first time. We intend to use this coating for FESEM observation of a variety of specimens.We are familiar with gold and gold-palladium targets but never used a chromium target before. This morning we coated a sample with chromium, but seems like it didn't get any coating at all. I did see the plasma. When we use gold or gold-palladium in the same coater, it works fine and we can see the coating on the surface. Is it possible to see a color difference after Cr coating for 60-100 sec. We are using 40-60% power.I noticed the Cr target is much thicker than the gold target.
I will appreciate someone telling if we are doing something wrong or it is just our simple ignorance about chromium target. Any help will be very much appreciated.
Thanks in advance,
Soumitra
Soumitra Ghoshroy EM Center University of South Carolina Columbia, SC 29208
==============================Original Headers============================== 20, 24 -- From protrain-at-emcourses.com Fri Feb 28 08:03:44 2014 20, 24 -- Received: from mail.esentra.net (mail.esentra.net [94.228.36.208]) 20, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SE3h0X023155 20, 24 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 08:03:44 -0600 20, 24 -- Received: from mail.clientmail.net (Not Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway 20, 24 -- id {B5310973e0000} ; Fri, 28 Feb 2014 14:03:42 +0000 20, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Fri, 28 Feb 2014 14:03:41 +0000 20, 24 -- Reply-To: {protrain-at-emcourses.com} 20, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} 20, 24 -- To: {ghoshroy-at-sc.edu} 20, 24 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 20, 24 -- References: {201402281325.s1SDPkKA001008-at-ns.microscopy.com} 20, 24 -- In-Reply-To: {201402281325.s1SDPkKA001008-at-ns.microscopy.com} 20, 24 -- Subject: RE: [Microscopy] viaWWW: Chromium sputtering target 20, 24 -- Date: Fri, 28 Feb 2014 14:03:41 -0000 20, 24 -- Organization: Protrain 20, 24 -- Message-ID: {001d01cf348d$e3309a00$a991ce00$-at-com} 20, 24 -- MIME-Version: 1.0 20, 24 -- Content-Type: text/plain; 20, 24 -- charset="us-ascii" 20, 24 -- Content-Transfer-Encoding: 7bit 20, 24 -- X-Mailer: Microsoft Office Outlook 12.0 20, 24 -- Thread-Index: Ac80iJZe8ZT+E30bQTOloqRWS7R+/AAA2JOg 20, 24 -- Content-Language: en-gb ==============================End of - Headers==============================
==============================Original Headers============================== 29, 26 -- From prvs=129e7d229=john.robson-at-boehringer-ingelheim.com Fri Feb 28 09:05:22 2014 29, 26 -- Received: from mail4.boehringer-ingelheim.com (mail4.boehringer-ingelheim.com [148.188.192.93]) 29, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SF5M5T029190 29, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 09:05:22 -0600 29, 26 -- Received: from NAHEXMB02.am.boehringer.com ([fe80::b19b:78f1:6b5f:e306]) by 29, 26 -- NAHEXCH02.am.boehringer.com ([fe80::d9b9:37f3:58a9:fb6%12]) with mapi id 29, 26 -- 14.02.0387.000; Fri, 28 Feb 2014 10:05:21 -0500 29, 26 -- From: {john.robson-at-boehringer-ingelheim.com} 29, 26 -- To: {Microscopy-at-microscopy.com} 29, 26 -- CC: {ghoshroy-at-sc.edu} 29, 26 -- Subject: RE: [Microscopy] RE: viaWWW: Chromium sputtering target 29, 26 -- Thread-Topic: [Microscopy] RE: viaWWW: Chromium sputtering target 29, 26 -- Thread-Index: AQHPNI83iyZcdqcy10meMJHXITB2dprKwrnA 29, 26 -- Date: Fri, 28 Feb 2014 15:05:21 +0000 29, 26 -- Message-ID: {0505FBCD63EE71428E13EBEEFC2C4E432275AC45-at-NAHEXMB02.am.boehringer.com} 29, 26 -- References: {201402281413.s1SEDBj3002903-at-ns.microscopy.com} 29, 26 -- In-Reply-To: {201402281413.s1SEDBj3002903-at-ns.microscopy.com} 29, 26 -- Accept-Language: en-US 29, 26 -- Content-Language: en-US 29, 26 -- X-MS-Has-Attach: 29, 26 -- X-MS-TNEF-Correlator: 29, 26 -- x-originating-ip: [10.128.230.253] 29, 26 -- Content-Type: text/plain; charset="us-ascii" 29, 26 -- MIME-Version: 1.0 29, 26 -- Content-Transfer-Encoding: 8bit 29, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1SF5M5T029190 ==============================End of - Headers==============================
Soumitra; Chromium is extremely reactive with oxygen. Even with a UHV system, chromium deposits tend to be chromium oxide. The best way to deposit chromium is with an ion beam sputtering system. Plasma sputtering, as you have discovered, does not work well for chromium. You see the glow of the plasma, but not much else happens.
John Mardinly, ASU
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, February 28, 2014 6:43 AM To: John Mardinly
X-from: ghoshroy-at-sc.edu ()
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We have Denton DESK II Turbo Sputter Coater and are planning to use a chromium sputtering target for the first time. We intend to use this coating for FESEM observation of a variety of specimens.We are familiar with gold and gold-palladium targets but never used a chromium target before. This morning we coated a sample with chromium, but seems like it didn't get any coating at all. I did see the plasma. When we use gold or gold-palladium in the same coater, it works fine and we can see the coating on the surface. Is it possible to see a color difference after Cr coating for 60-100 sec. We are using 40-60% power.I noticed the Cr target is much thicker than the gold target.
I will appreciate someone telling if we are doing something wrong or it is just our simple ignorance about chromium target. Any help will be very much appreciated.
Thanks in advance,
Soumitra
Soumitra Ghoshroy EM Center University of South Carolina Columbia, SC 29208
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==============================Original Headers============================== 24, 37 -- From John.Mardinly-at-asu.edu Fri Feb 28 13:36:04 2014 24, 37 -- Received: from bcnet7.asu.edu (bcnet7.asu.edu [129.219.117.222]) 24, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SJa4E3019852 24, 37 -- for {Microscopy-at-Microscopy.com} ; Fri, 28 Feb 2014 13:36:04 -0600 24, 37 -- X-ASG-Debug-ID: 1393616163-04dc7a6b0b3916c0001-FOsErg 24, 37 -- Received: from exhubt01.asurite.ad.asu.edu (exhubt01.asurite.ad.asu.edu [129.219.103.40]) by bcnet7.asu.edu with ESMTP id A1To56V6e2csQrh4 (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Fri, 28 Feb 2014 12:36:03 -0700 (MST) 24, 37 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 24, 37 -- X-Barracuda-Apparent-Source-IP: 129.219.103.40 24, 37 -- X-ASG-Whitelist: Client 24, 37 -- Received: from EXMBW02.asurite.ad.asu.edu ([169.254.9.176]) by 24, 37 -- exhubt01.asurite.ad.asu.edu ([129.219.103.40]) with mapi id 14.03.0174.001; 24, 37 -- Fri, 28 Feb 2014 12:36:03 -0700 24, 37 -- From: John Mardinly {John.Mardinly-at-asu.edu} 24, 37 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 24, 37 -- Subject: RE: [Microscopy] viaWWW: Chromium sputtering target 24, 37 -- Thread-Topic: [Microscopy] viaWWW: Chromium sputtering target 24, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] viaWWW: Chromium sputtering target 24, 37 -- Thread-Index: AQHPNIsH04ggBj0lsE2ks93J6PhfGprLDhfg 24, 37 -- Date: Fri, 28 Feb 2014 19:36:02 +0000 24, 37 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE940115E89F1-at-exmbw02.asurite.ad.asu.edu} 24, 37 -- References: {201402281343.s1SDhAdA005717-at-ns.microscopy.com} 24, 37 -- In-Reply-To: {201402281343.s1SDhAdA005717-at-ns.microscopy.com} 24, 37 -- Accept-Language: en-US 24, 37 -- Content-Language: en-US 24, 37 -- X-MS-Has-Attach: 24, 37 -- X-MS-TNEF-Correlator: 24, 37 -- x-originating-ip: [129.219.4.240] 24, 37 -- Content-Type: text/plain; charset="us-ascii" 24, 37 -- MIME-Version: 1.0 24, 37 -- X-Barracuda-Connect: exhubt01.asurite.ad.asu.edu[129.219.103.40] 24, 37 -- X-Barracuda-Start-Time: 1393616163 24, 37 -- X-Barracuda-Encrypted: AES128-SHA 24, 37 -- X-Barracuda-URL: http://129.219.117.222:8000/cgi-mod/mark.cgi 24, 37 -- X-Virus-Scanned: by bsmtpd at asu.edu 24, 37 -- X-Barracuda-BRTS-Status: 1 24, 37 -- Content-Transfer-Encoding: 8bit 24, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1SJa4E3019852 ==============================End of - Headers==============================
On behalf of the organizing committee, I’d like to extend an invitation for you to attend the first Microscopy of Infectious Disease Agents Symposia we are hosting at Rocky Mountain Laboratories/NIAID/NIH in Hamilton, Montana July 24th and 25th. Please feel free to pass this along to anyone who might be interested in attending.
The 2014 Microscopy of Infectious Disease Agents Symposia (MIDAS) will provide an opportunity to bring together leading researchers of microscopic imaging and related techniques as they apply to the study of human pathogens. Organized by the National Institute of Allergy of Infectious Diseases, National Institutes of Health, this meeting will provide a venue for recent progress in areas of both light and electron microscopies relevant to imaging viruses, bacteria, prions, host cells and tissues. In addition to scientific presentations, workshops will be organized to teach various aspects of emerging technologies. The field of microscopic imaging is rapidly evolving as advances in both instrumentation and computational sciences are enabling a greatly expanding range of analyses to be performed. A broad theme at the conference will be on technological advances and on future developments needed to increase the application and impact of microscopic imaging for understanding the biology of human pathogens with the goal of improving the treatment, diagnosis, and prevention of infectious diseases. The conference will bring together a diverse group of scientists at senior and junior levels. Seminars and posters at the conference will provide an opportunity to present research findings and exchange of ideas.
Please visit our registration site for more information and the preliminary agenda. respond.niaid.nih.gov/conferences/rmlsymposium2014/Pages
Keynote speakers:
Sriram Subramaniam, Ph.D., National Cancer Institute, NIH 
Dr. Sriram Subramaniam received his Ph.D. in Physical Chemistry from Stanford University and completed postdoctoral training in the Departments of Chemistry and Biology at M.I.T. He is chief of the Biophysics Section in the Laboratory of Cell Biology at the Center for Cancer Research, National Cancer Institute. He holds a visiting faculty appointment at the Johns Hopkins University School of Medicine. His current work is focused on the development of advanced technologies for imaging macromolecular assemblies using 3D electron microscopy, and their application to address fundamental problems in HIV/AIDS and cancer research. Hari Shroff , Ph.D., National Ins titute of Biomedical Imaging and Bioengineering, NIH Dr. Hari Shroff received a B.S.E. in bioengineering from the University of Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed his Ph.D. in biophysics at the University of California at Berkeley in 2006. He spent the next three years performing postdoctoral research under the mentorship of Eric Betzig at the Howard Hughes Medical Institute's Janelia Farm Research Campus where his research focused on development of photo-activated localization microscopy (PALM), an optical super resolution technique. Dr. Shroff is now chief of NIBIB's section on high resolution optical imaging laboratory, where he and his staff are developing new imaging tools for application in biological and clinical research. Dr Shroff’s lab has recently developed two new microscope systems: The first, iSIM, uses structured illumination and high speed processing to render images with double the resolution of conventional fluorescence microscope. The second, diSPIM, is a light sheet microscope which uses two objectives to achieve isotropic resolution with minimal bleaching.
Organizing committee: Elizabeth Fischer, NIH/NIAID, Audray Harris, NIH/NIAID, and Owen Schwartz, NIH/NIAID
Contact: Elizabeth R. Fischer Microscopy Unit Manager Research Technologies Section/RTB Rocky Mountain Laboratories/NIAID/NIH 903 South 4th Street Hamilton, MT 59840 406-363-9378
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Email: tina.williams-at-ars.usda.gov Name: Tina Williams
Organization: USDA
Title-Subject: [Filtered] vacuum oven polymerization: Vent to hood?
Message: Hello,
While polymerizing your resins, do you vent your oven in a fume hood or to the lab? Safety suggestions? Thank you, Tina
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 28 18:19:10 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s210JAck028596 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 18:19:10 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 638116C7EEB 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 18:19:10 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id Rq334j911JgS for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 28 Feb 2014 18:19:08 -0600 (CST) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id BAFDF6C7EDE 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 18:19:08 -0600 (CST) 14, 28 -- Message-ID: {5311277C.5020807-at-microscopy.com} 14, 28 -- Date: Fri, 28 Feb 2014 18:19:08 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Question: vacuum oven polymerization: Vent to hood? 14, 28 -- References: {201402282342.s1SNggdX009382-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201402282342.s1SNggdX009382-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201402282342.s1SNggdX009382-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
PITTCON is not typically considered an important venue for microscopy, but there will be at least three dozen microscopy and related technology companies exhibiting at Chicago's McCormick Center, March 2-6. In addition, there will be a number of seminars, tutorials, and lunch&learn opportunities. American Lab asked that I do a review of Microscopy at PITTCON. That article is now posted at:
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realname - Pamela Coker, Ph.D. Email - pjcoker-at-pima.edu ORGANIZATION - Ph.D. EDUCATION - Undergraduate College LOCATION - Tucson, AZ 85743 SUBJECT_OF_QUESTION - Looking for a reference slide set for pharmaceuticals QUESTION - Hello,
I am writing a proposal for a new course in forensic microscopy. I have found most of the reference slide sets I need, however I am coming up empty handed on a pharmaceutical slide set.
Can anyone point me in the right direction? I'm not finding one at McCrone's. Thanks, Dr. C.
==============================Original Headers============================== 6, 27 -- From oshel1pe-at-cmich.edu Mon Mar 3 07:18:10 2014 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s23DIAXS002597 6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 07:18:10 -0600 6, 27 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s23DHuSw009468 6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 08:18:09 -0500 6, 27 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 6, 27 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 6, 27 -- 14.3.174.1; Mon, 3 Mar 2014 08:18:04 -0500 6, 27 -- Message-ID: {5314810C.3040809-at-cmich.edu} 6, 27 -- Date: Mon, 3 Mar 2014 08:18:04 -0500 6, 27 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 6, 27 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 27 -- MIME-Version: 1.0 6, 27 -- To: micro {Microscopy-at-microscopy.com} 6, 27 -- Subject: Ask-A-Microscopist: Reference slide set for pharmaceuticals needed 6, 27 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 27 -- Content-Transfer-Encoding: 7bit 6, 27 -- X-Originating-IP: [141.209.160.122] 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 27 -- X-Spam-Score: -0.50 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 6, 27 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 27 -- X-CanItPRO-Stream: default 6, 27 -- X-Canit-Stats-ID: 02Lx1i9P6 - 0ff8d5c59c4d - 20140303 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I was wondering if there are digital versions of the user manuals for the Hitachi 7000 transmission EM and Hitachi S-3000N scanning EM? If so, I would very much appreciate a copy of them. Please contact me off-line and we can see how this can be arranged.
Many thanks, Debby
Debra Sherman, Founder & CSO DS imaging LLC Purdue Kurz Technology Center 1281 Win Hentschel Blvd West Lafayette, IN 47906 E-mail: debby.sherman-at-dsimagingllc.com www.dsimagingllc.com Mobile: 765-418-8540
==============================Original Headers============================== 8, 31 -- From dsherman-at-purdue.edu Mon Mar 3 11:37:59 2014 8, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s23Hbx7s024993 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 11:37:59 -0600 8, 31 -- Received: from WPVEXCHUB03.purdue.lcl (wpvexchub03.itap.purdue.edu [172.30.136.67]) 8, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s23Hbw4t008090 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 12:37:59 -0500 8, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([169.254.2.185]) by 8, 31 -- WPVEXCHUB03.purdue.lcl ([172.30.136.67]) with mapi id 14.03.0174.001; Mon, 3 8, 31 -- Mar 2014 12:37:58 -0500 8, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 8, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 31 -- Subject: Hitachi user manuals 8, 31 -- Thread-Topic: Hitachi user manuals 8, 31 -- Thread-Index: AQHPNwdRNT7eHhEgJ0W48koLW7AExg== 8, 31 -- Date: Mon, 3 Mar 2014 17:37:58 +0000 8, 31 -- Message-ID: {CF3A287D.1AC9F%dsherman-at-purdue.edu} 8, 31 -- Accept-Language: en-US 8, 31 -- Content-Language: en-US 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 8, 31 -- x-originating-ip: [24.14.170.252] 8, 31 -- Content-Type: text/plain; charset="us-ascii" 8, 31 -- Content-ID: {8C5FED44FF784340AB2715322C23B6F5-at-exchange.purdue.edu} 8, 31 -- MIME-Version: 1.0 8, 31 -- X-PMX-Version: 6.0.2.2308539 8, 31 -- X-PerlMx-URL-Scanned: Yes 8, 31 -- X-PerlMx-Virus-Scanned: Yes 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s23Hbx7s024993 ==============================End of - Headers==============================
Trying to address an electronics high temperature error on our Leo 906E TEM.
Would like to know where the temperature sensor is located on the system (column chassis or power supply rack) in order to troubleshoot the problem further.
Have thought for some time the issue was with the chiller but its running at 58 degrees F at a flow rate of 54 gph and the room is 70 degrees F.
On our coldest days this winter we have successfully run the instrument but we had a warm spell last week and the problem reappeared.
All rack cooling fans are running and filters are clean.
Any ideas are appreciated.
Roy Beavers Southern Methodist University rbeavers-at-smu.edu
==============================Original Headers============================== 10, 28 -- From rbeavers-at-mail.smu.edu Mon Mar 3 14:13:36 2014 10, 28 -- Received: from smtap2.systems.smu.edu (smtap2.systems.smu.edu [129.119.65.151]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s23KDavv007153 10, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 14:13:36 -0600 10, 28 -- X-IronPort-Anti-Spam-Filtered: true 10, 28 -- X-IronPort-Anti-Spam-Result: AvsMAI7hFFOBd0GH/2dsb2JhbABagmUhgRKzUDqMUYEmFnSCJwU6UQEqFEImAQQTCIdxAZ0UqUCGBReOCx2DXIEUBJRRlhaDLYIq 10, 28 -- X-IPAS-Result: AvsMAI7hFFOBd0GH/2dsb2JhbABagmUhgRKzUDqMUYEmFnSCJwU6UQEqFEImAQQTCIdxAZ0UqUCGBReOCx2DXIEUBJRRlhaDLYIq 10, 28 -- Received: from sxht1p4.systems.smu.edu ([129.119.65.135]) 10, 28 -- by smtah2.systems.smu.edu with ESMTP/TLS/AES128-SHA; 03 Mar 2014 14:13:36 -0600 10, 28 -- Received: from SXMB1PG.SYSTEMS.SMU.EDU ([fe80::1449:c186:5adb:ddbb]) by 10, 28 -- SXHT1P4.SYSTEMS.SMU.EDU ([fe80::4003:49d5:389b:a18%26]) with mapi id 10, 28 -- 14.02.0387.000; Mon, 3 Mar 2014 14:13:35 -0600 10, 28 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 10, 28 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 10, 28 -- Subject: Leo 906E TEM Temperature Issue 10, 28 -- Thread-Topic: Leo 906E TEM Temperature Issue 10, 28 -- Thread-Index: Ac83Gpswb/X1mDg2SCa+e+CZHSoYkw== 10, 28 -- Date: Mon, 3 Mar 2014 20:13:34 +0000 10, 28 -- Message-ID: {BC0C36E74D341741A25CE2ABB122E7A330B0B323-at-SXMB1PG.SYSTEMS.SMU.EDU} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- x-originating-ip: [129.119.65.139] 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s23KDavv007153 ==============================End of - Headers==============================
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Email: tina.williams-at-ars.usda.gov Name: Tina Williams
Organization: USDA
Title-Subject: [Filtered] vacuum oven polymerization: Vent to hood- Thanks for all the replies/suggestions
Message: Hello again,
Thank you to all who replied to my question regarding venting in a fume hood during polymerization of resins. I especially appreciated the references and safety reminders to reduce respiratory/skin/immuno sensitivities by venting in a fume hood.
I wanted to confirm, by having your collective experiences working with resins, that we continue working in harmony with these safety protocols. We have been venting the resin polymerization in the fume hood. However our current EHS officer, brought up the concern regarding the oven effecting the airflow in the hood. Since we have a large/heavy oven, and the same issue may apply with a small oven (if one could be found), we have kept the oven in the hood.
Your answers help us have a basis to continue with our current safety protocols, until a safer alternative is found.
Tina
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==============================Original Headers============================== 16, 29 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 3 18:49:21 2014 16, 29 -- Received: from znl.com ([206.69.208.20]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240nL3E009797 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:49:21 -0600 16, 29 -- Received: from localhost (localhost [127.0.0.1]) 16, 29 -- by znl.com (Postfix) with ESMTP id 1DB686CA2A4 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:49:21 -0600 (CST) 16, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 29 -- Received: from znl.com ([127.0.0.1]) 16, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 29 -- with ESMTP id Ir3pTYull+03 for {microscopy-at-microscopy.com} ; 16, 29 -- Mon, 3 Mar 2014 18:49:17 -0600 (CST) 16, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 29 -- by znl.com (Postfix) with ESMTPA id C67D36CA297 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:49:17 -0600 (CST) 16, 29 -- Message-ID: {5315230D.2080505-at-microscopy.com} 16, 29 -- Date: Mon, 03 Mar 2014 18:49:17 -0600 16, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 16, 29 -- MIME-Version: 1.0 16, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 29 -- Subject: viaWWW: vacuum oven polymerization: Vent to hood- Thanks for all 16, 29 -- the replies/suggestions 16, 29 -- References: {201403031952.s23JqWuL004732-at-ns.microscopy.com} 16, 29 -- In-Reply-To: {201403031952.s23JqWuL004732-at-ns.microscopy.com} 16, 29 -- X-Forwarded-Message-Id: {201403031952.s23JqWuL004732-at-ns.microscopy.com} 16, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: gr3g0rr33-at-gmail.com Name: Gregory Wyche
Organization: University of South Carolina
Title-Subject: [Filtered] Networking Opportunities In CT
Message: Hi, I'm a microscopist in CT (currently between jobs) looking for opportunities to network in that state. The Connecticut Microscopy Society website has not been updated in a long time so I can't get in touch with any of the members. If you know of any groups that meet, please forward them. I'll very much appreciate it.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 3 18:50:02 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240o2kB010547 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:02 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id 768ED6CA2B3 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:02 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id TlwPJ2eaIOP0 for {microscopy-at-microscopy.com} ; 12, 28 -- Mon, 3 Mar 2014 18:50:01 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id 36DBC6CA2A6 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:01 -0600 (CST) 12, 28 -- Message-ID: {53152339.7040806-at-microscopy.com} 12, 28 -- Date: Mon, 03 Mar 2014 18:50:01 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Networking Opportunities In CT 12, 28 -- References: {201403032055.s23Ktm2d026609-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201403032055.s23Ktm2d026609-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201403032055.s23Ktm2d026609-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: dvanhart-at-binghamton.edu Name: Daniel VanHart
Organization: Binghamton University
Title-Subject: [Filtered] FTIR Microscope
Message: Does anyone have any experience with a Pike Technologies microMax Microscope?
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 3 18:52:36 2014 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240qYAR014362 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:52:34 -0600 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 49F996CA2C8 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:52:10 -0600 (CST) 11, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id R9rBs7d99M+l for {microscopy-at-microscopy.com} ; 11, 28 -- Mon, 3 Mar 2014 18:50:45 -0600 (CST) 11, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 024666CA2B5 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:45 -0600 (CST) 11, 28 -- Message-ID: {53152364.1080909-at-microscopy.com} 11, 28 -- Date: Mon, 03 Mar 2014 18:50:44 -0600 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:FTIR Microscope 11, 28 -- References: {201403032103.s23L3jd4026772-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201403032103.s23L3jd4026772-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201403032103.s23L3jd4026772-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================