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==============================Original Headers============================== 20, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 1 08:41:49 2014 20, 25 -- Received: from znl.com ([206.69.208.20]) 20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s01EfnJu013746 20, 25 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jan 2014 08:41:49 -0600 20, 25 -- Received: from localhost (localhost [127.0.0.1]) 20, 25 -- by znl.com (Postfix) with ESMTP id 7728769F1F8 20, 25 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jan 2014 08:41:49 -0600 (CST) 20, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 20, 25 -- Received: from znl.com ([127.0.0.1]) 20, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 20, 25 -- with ESMTP id 2aSIXMr187vE for {microscopy-at-microscopy.com} ; 20, 25 -- Wed, 1 Jan 2014 08:41:44 -0600 (CST) 20, 25 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 20, 25 -- by znl.com (Postfix) with ESMTPA id 1F85F69F1E8 20, 25 -- for {microscopy-at-microscopy.com} ; Wed, 1 Jan 2014 08:41:44 -0600 (CST) 20, 25 -- Message-ID: {52C42927.8020603-at-microscopy.com} 20, 25 -- Date: Wed, 01 Jan 2014 08:41:43 -0600 20, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 25 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 25 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 20, 25 -- MIME-Version: 1.0 20, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 25 -- Subject: Administrivia: Happy New Year 2014 20, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 20, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Could anyone give me an advice about the FE-SEM JSM-7100FT? Is it a good equipment for working with metal alloys, steels, considering I must have good images in a variety of microstructures, good and fast texture analysis (EBSD) and EDX? Can it be placed on the same level as the ZEISS Sigma HD?
I don't know any institution where there is such a microscope - FE-SEM JSM-7100FT - in function. Do you?
Thanks in advance
Margareth
Technology Center Institute of Metalurgy and Special Alloys Brazil
==============================Original Headers============================== 7, 46 -- From margareth.andrade-at-fiemg.com.br Thu Jan 2 06:58:32 2014 7, 46 -- Received: from co9outboundpool.messaging.microsoft.com (co9ehsobe003.messaging.microsoft.com [207.46.163.26]) 7, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s02CwVCl015853 7, 46 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 06:58:31 -0600 7, 46 -- Received: from mail160-co9-R.bigfish.com (10.236.132.229) by 7, 46 -- CO9EHSOBE032.bigfish.com (10.236.130.95) with Microsoft SMTP Server id 7, 46 -- 14.1.225.22; Thu, 2 Jan 2014 12:58:26 +0000 7, 46 -- Received: from mail160-co9 (localhost [127.0.0.1]) by 7, 46 -- mail160-co9-R.bigfish.com (Postfix) with ESMTP id 9B9CD240094 for 7, 46 -- {Microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 12:58:26 +0000 (UTC) 7, 46 -- X-Forefront-Antispam-Report: CIP:132.245.156.37;KIP:(null);UIP:(null);IPV:NLI;H:GRXPRD8010HT004.lamprd80.prod.outlook.com;RD:none;EFVD:NLI 7, 46 -- X-SpamScore: -1 7, 46 -- X-BigFish: PS-1(zz1443Izz1f42h2148h208ch1ee6h1de0h1d18h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah1fc6hzzz2fh109h2a8h839h944hd25hf0ah1220h1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh15d0h162dh1631h1758h18e1h1946h19b5h19ceh1ad9h1b0ah224fh1d07h1d0ch1d2eh1d3fh1de9h1dfeh1dffh1e1dh1fe8h1ff5h2216h22d0h2336h1155h) 7, 46 -- Received-SPF: pass (mail160-co9: domain of fiemg.com.br designates 132.245.156.37 as permitted sender) client-ip=132.245.156.37; envelope-from=margareth.andrade-at-fiemg.com.br; helo=GRXPRD8010HT004.lamprd80.prod.outlook.com ;.outlook.com ; 7, 46 -- Received: from mail160-co9 (localhost.localdomain [127.0.0.1]) by mail160-co9 7, 46 -- (MessageSwitch) id 1388667503935090_11462; Thu, 2 Jan 2014 12:58:23 +0000 7, 46 -- (UTC) 7, 46 -- Received: from CO9EHSMHS009.bigfish.com (unknown [10.236.132.227]) by 7, 46 -- mail160-co9.bigfish.com (Postfix) with ESMTP id D317A38004C for 7, 46 -- {Microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 12:58:23 +0000 (UTC) 7, 46 -- Received: from GRXPRD8010HT004.lamprd80.prod.outlook.com (132.245.156.37) by 7, 46 -- CO9EHSMHS009.bigfish.com (10.236.130.19) with Microsoft SMTP Server (TLS) id 7, 46 -- 14.16.227.3; Thu, 2 Jan 2014 12:58:18 +0000 7, 46 -- Received: from GRXPRD8010MB526.lamprd80.prod.outlook.com ([169.254.10.185]) by 7, 46 -- GRXPRD8010HT004.lamprd80.prod.outlook.com ([10.242.26.37]) with mapi id 7, 46 -- 14.16.0395.000; Thu, 2 Jan 2014 12:58:11 +0000 7, 46 -- From: Margareth Spangler Andrade {margareth.andrade-at-fiemg.com.br} 7, 46 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 46 -- Subject: info on new JEOL 7, 46 -- Thread-Topic: info on new JEOL 7, 46 -- Thread-Index: Ac8HWS/Tlwh1lgE9TtWXDZDN2Ktc4AAYCnES 7, 46 -- Date: Thu, 2 Jan 2014 12:58:11 +0000 7, 46 -- Message-ID: {F96AE27C3117434CB0E8CB6C2EE9DA5429D81BF3-at-GRXPRD8010MB526.lamprd80.prod.outlook.com} 7, 46 -- References: {F96AE27C3117434CB0E8CB6C2EE9DA5429D81BC7-at-GRXPRD8010MB526.lamprd80.prod.outlook.com} 7, 46 -- In-Reply-To: {F96AE27C3117434CB0E8CB6C2EE9DA5429D81BC7-at-GRXPRD8010MB526.lamprd80.prod.outlook.com} 7, 46 -- Accept-Language: pt-BR, en-US 7, 46 -- Content-Language: pt-BR 7, 46 -- X-MS-Has-Attach: 7, 46 -- X-MS-TNEF-Correlator: 7, 46 -- x-originating-ip: [201.80.92.47] 7, 46 -- Content-Type: text/plain; charset="us-ascii" 7, 46 -- MIME-Version: 1.0 7, 46 -- X-OriginatorOrg: fiemg.com.br 7, 46 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 7, 46 -- Content-Transfer-Encoding: 8bit 7, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s02CwVCl015853 ==============================End of - Headers==============================
The GIF on our CM200FEG is now refusing to tune its isochromatic surface. I tried all of the obvious easy fixes, such as making sure I was on vacuum, acquiring a new gain reference, acquiring a new dark reference, reloading the last known good GIB alignment (~3 weeks old), and rebooting everything that can be booted (PC, GIB, Digiscan).
The error message is along the lines of "Too many defects in the isochromatic surface."
Any thoughts on what I might try? It's conceivable that a 20-year-old GIF has decided to fail; any way I could diagnose if a DAC or lens has decided to die on us?
Thanks Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 8, 30 -- From parishcm-at-ornl.gov Thu Jan 2 14:18:13 2014 8, 30 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s02KICpE009801 8, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 14:18:12 -0600 8, 30 -- X-SG: RELAYLIST 8, 30 -- X-IronPort-AV: E=Sophos;i="4.95,592,1384318800"; 8, 30 -- d="scan'208";a="53361480" 8, 30 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 8, 30 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 02 Jan 2014 15:18:11 -0500 8, 30 -- Received: from exchhub2.ornl.gov (exchhub2.ornl.gov [160.91.2.112]) 8, 30 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 8A4AEE0296 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Jan 2014 15:18:11 -0500 (EST) 8, 30 -- Received: from EXCHMBB.ornl.gov ([192.168.3.201]) by exchhub2.ornl.gov 8, 30 -- ([160.91.2.112]) with mapi; Thu, 2 Jan 2014 15:18:11 -0500 8, 30 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 8, 30 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 8, 30 -- Date: Thu, 2 Jan 2014 15:18:10 -0500 8, 30 -- Subject: EFTEM: GIF 678 won't tune 8, 30 -- Thread-Topic: EFTEM: GIF 678 won't tune 8, 30 -- Thread-Index: Ac8H98Bgh2q5tr7kTZOlENmCkwonHw== 8, 30 -- Message-ID: {3C47B404D340EE48A469ADAE2ADBF96676C1492CE6-at-EXCHMBB.ornl.gov} 8, 30 -- Accept-Language: en-US 8, 30 -- Content-Language: en-US 8, 30 -- X-MS-Has-Attach: 8, 30 -- X-MS-TNEF-Correlator: 8, 30 -- acceptlanguage: en-US 8, 30 -- Content-Type: text/plain; charset="us-ascii" 8, 30 -- MIME-Version: 1.0 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s02KICpE009801 ==============================End of - Headers==============================
Thanks to Pang, who had an eyepiece with a reticle. I just had to walk across campus and take it apart. The Periplan GF takes a reticle of 18.5 mm diameter inserted into the focusing eyepiece, not the fixed focus eyepiece. Remove the lower lens assembly (black) and then unscrew a small threaded ring from the “upper” end of the assembly. the reticle nests on a recessed ledge and held in place by the threaded ring.
Cynthia’s links are very useful for these older Leitz scopes.
Regards, Glen Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Cellular Morphology Core Center on Human Development and Disability Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-uw.edu
--------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } A colleague has a gray Leitz inverted microscope with Periplan GF 12.5X eyepieces, 23.2 mm outside diameter. Does anyone know whether they will take an eyepiece reticle? } Thanks, } Glen MacDonald } Core for Communication Research } Virginia Merrill Bloedel Hearing Research Center } Cellular Morphology Core } Center on Human Development and Disability } Box 357923 } University of Washington } Seattle, WA 98195-7923 USA } (206) 616-4156 } glenmac-at-uw.edu } } } } } } } } } } ==============================Original Headers============================== } 9, 27 -- From glenmac-at-u.washington.edu Tue Dec 31 10:44:24 2013 } 9, 27 -- Received: from mxout12.cac.washington.edu (mxout12.cac.washington.edu [140.142.32.167]) } 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id rBVGiO2e030605 } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 31 Dec 2013 10:44:24 -0600 } 9, 27 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.170]) } 9, 27 -- by mxout12.cac.washington.edu (8.14.4+UW11.03/8.14.4+UW13.09) with ESMTP id rBVGextf024220 } 9, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 31 Dec 2013 08:40:59 -0800 } 9, 27 -- X-Auth-Received: from d-128-208-204-174.dhcp4.washington.edu (D-128-208-204-174.dhcp4.washington.edu [128.208.204.174]) } 9, 27 -- (authenticated authid=glenmac) } 9, 27 -- by smtp.washington.edu (8.14.4+UW11.03/8.14.4+UW13.09) with ESMTP id rBVGew7H031750 } 9, 27 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 31 Dec 2013 08:40:58 -0800 } 9, 27 -- From: Glen MacDonald {glenmac-at-u.washington.edu} } 9, 27 -- Content-Type: text/plain; charset=us-ascii } 9, 27 -- Subject: Leitz periplan reticles } 9, 27 -- Message-Id: {EA6FCF59-6721-4EB0-A86E-A3255006651A-at-u.washington.edu} } 9, 27 -- Date: Tue, 31 Dec 2013 08:40:57 -0800 } 9, 27 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} } 9, 27 -- Mime-Version: 1.0 (Mac OS X Mail 7.0 \(1822\)) } 9, 27 -- X-Mailer: Apple Mail (2.1822) } 9, 27 -- X-PMX-Version: 6.0.0.2142326, Antispam-Engine: 2.7.2.2107409, Antispam-Data: 2013.12.31.163318 } 9, 27 -- X-PMX-Server: mxout12.cac.washington.edu } 9, 27 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report=' } 9, 27 -- HTML_00_01 0.05, HTML_00_10 0.05, MIME_LOWER_CASE 0.05, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_400_499 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, __ANY_URI 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_FROM 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __MSGID_APPLEMAIL 0, __SANE_MSGID 0, __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NO_PATH 0, __URI_NO_WWW 0, __URI_NS , __USER_AGENT_APPLEMAIL 0, __X_MAILER_APPLEMAIL 0' } 9, 27 -- Content-Transfer-Encoding: 8bit } 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id rBVGiO2e030605 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 29 -- From glenmac-at-u.washington.edu Thu Jan 2 14:24:03 2014 9, 29 -- Received: from mxout14.cac.washington.edu (mxout14.cac.washington.edu [140.142.32.168]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s02KO2o2014492 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 14:24:03 -0600 9, 29 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.205]) 9, 29 -- by mxout14.cac.washington.edu (8.14.4+UW11.03/8.14.4+UW13.09) with ESMTP id s02KNq9f013486 9, 29 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 12:23:52 -0800 9, 29 -- X-Auth-Received: from d-128-208-187-41.dhcp4.washington.edu (D-128-208-187-41.dhcp4.washington.edu [128.208.187.41]) 9, 29 -- (authenticated authid=glenmac) 9, 29 -- by smtp.washington.edu (8.14.4+UW11.03/8.14.4+UW13.09) with ESMTP id s02KNpEC027040 9, 29 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jan 2014 12:23:52 -0800 9, 29 -- Content-Type: text/plain; charset=windows-1252 9, 29 -- Mime-Version: 1.0 (Mac OS X Mail 7.0 \(1822\)) 9, 29 -- Subject: Re: [Microscopy] Leitz periplan reticles 9, 29 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 9, 29 -- In-Reply-To: {201312311646.rBVGkOnC032160-at-ns.microscopy.com} 9, 29 -- Date: Thu, 2 Jan 2014 12:23:51 -0800 9, 29 -- Message-Id: {011012E8-2A7F-4D0B-8C77-3BF570D951B4-at-u.washington.edu} 9, 29 -- References: {201312311646.rBVGkOnC032160-at-ns.microscopy.com} 9, 29 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 9, 29 -- X-Mailer: Apple Mail (2.1822) 9, 29 -- X-PMX-Version: 6.0.0.2142326, Antispam-Engine: 2.7.2.2107409, Antispam-Data: 2014.1.2.201515 9, 29 -- X-PMX-Server: mxout14.cac.washington.edu 9, 29 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report=' 9, 29 -- HTML_00_01 0.05, HTML_00_10 0.05, MIME_LOWER_CASE 0.05, SUPERLONG_LINE 0.05, BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, URI_ENDS_IN_HTML 0, __ANY_URI 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FORWARDED_MSG 0, __HAS_FROM 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __IN_REP_TO 0, __LINES_OF_YELLING 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __MSGID_APPLEMAIL 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __SUBJ_ALPHA_END 0, __SUBJ_ALPHA_NEGATE 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS , __USER_AGENT_APPLEMAIL 0, __X_MAILER_APPLEMAIL 0' 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s02KO2o2014492 ==============================End of - Headers==============================
There are two possibilities, based on my experience with GIFs (I've used 5 different GIFs on 5 different instruments over the past 18 years):
1. The table of defective columns and rows has been lost (or modified) so that those genuinely defective pixels has not been correctly tabulated;
2. The Peltier cooler on the CCD camera is not cooling (at all).
On the balance of probability, I would suggest you investigate the second possibility first (the first would take some effort either by accident or malicious design).
We recently had problems with a GIF model 2000 on a CM300F which was commissioned in 1997. We had the same (or similar) error message. After contacting Gatan, they suggested estimating the temperature of the chip based on the dark count rate of two different exposure times (0.1 sec and 5.1 sec I think), taking the log of that ratio and dividing by some number (I cannot recall the exact figure- it is in my lab book back in the office- I'll try to dig it out when I return).
After this investigation, we found the Peltier cooler wasn't cooling the CCD chip. This was traced to a delicate copper wire that had broken that connected the Peltier PSU to the cooler. Gatan succeeded in fixing it (and getting the cooler to work at their offices), but did not work once it was reinstalled (we suspect the wire had broken again in transit). It is an open question as to whether repairing this again will fix the problem.
Anyway, I hope this helps in some small way.
Yours, Jon
Cambridge University, UK.
On 2014-01-02 20:27, parishcm-at-ornl.gov wrote:
} The GIF on our CM200FEG is now refusing to tune its isochromatic } surface. I tried all of the obvious easy fixes, such as making sure I } was on vacuum, acquiring a new gain reference, acquiring a new dark } reference, reloading the last known good GIB alignment (~3 weeks old), } and rebooting everything that can be booted (PC, GIB, Digiscan). } } The error message is along the lines of "Too many defects in the } isochromatic surface." } } Any thoughts on what I might try? It's conceivable that a 20-year-old } GIF has decided to fail; any way I could diagnose if a DAC or lens has } decided to die on us?
==============================Original Headers============================== 13, 31 -- From jsb43-at-cam.ac.uk Fri Jan 3 12:39:27 2014 13, 31 -- Received: from ppsw-52.csi.cam.ac.uk (ppsw-52.csi.cam.ac.uk [131.111.8.152]) 13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s03IdOr5031223 13, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Jan 2014 12:39:25 -0600 13, 31 -- X-Cam-AntiVirus: no malware found 13, 31 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 13, 31 -- Received: from webmail-2b.csi.cam.ac.uk ([131.111.9.35]:43068) 13, 31 -- by ppsw-52.csi.cam.ac.uk (ppsw.cam.ac.uk [131.111.8.138]:25) 13, 31 -- with esmtp id 1Vz9eV-0008Ge-FA (Exim 4.82_3-c0e5623) 13, 31 -- (return-path {jsb43-at-cam.ac.uk} ); Fri, 03 Jan 2014 18:39:23 +0000 13, 31 -- Received: from [127.0.0.1] (helo=webmail-2.hermes.cam.ac.uk) 13, 31 -- by webmail-2b.csi.cam.ac.uk with esmtp id 1Vz9eV-000qMM-KL (Exim 4.80_167-5a66dd3) 13, 31 -- (return-path {jsb43-at-cam.ac.uk} ); Fri, 03 Jan 2014 18:39:23 +0000 13, 31 -- Received: from host31-54-55-223.range31-54.btcentralplus.com ([31.54.55.223]) 13, 31 -- by webmail-2.hermes.cam.ac.uk 13, 31 -- with HTTP (HTTP/1.1 POST); Fri, 03 Jan 2014 18:39:23 +0000 13, 31 -- MIME-Version: 1.0 13, 31 -- Content-Type: text/plain; charset=UTF-8; 13, 31 -- format=flowed 13, 31 -- Content-Transfer-Encoding: 7bit 13, 31 -- X-Authenticated-User: jsb43 13, 31 -- Date: Fri, 03 Jan 2014 18:39:23 +0000 13, 31 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 13, 31 -- To: parishcm-at-ornl.gov 13, 31 -- Cc: Listserver MSA {Microscopy-at-microscopy.com} 13, 31 -- Subject: Re: EFTEM: GIF 678 won't tune 13, 31 -- In-Reply-To: {201401022027.s02KRo7r024182-at-ns.microscopy.com} 13, 31 -- References: {201401022027.s02KRo7r024182-at-ns.microscopy.com} 13, 31 -- Message-ID: {05d76d09370056d66680b787c8127d21-at-cam.ac.uk} 13, 31 -- X-Sender: jsb43-at-cam.ac.uk 13, 31 -- User-Agent: Roundcube Webmail/0.9.4 ==============================End of - Headers==============================
We are pleased to announce that the first meeting of the Chesapeake Microscopy and Microanalysis Society will be held on January 14th, 2014. We are very excited to have Dale Greenwalt of the Smithsonian National Museum of Natural History as our speaker that evening. If you are in the area of Bethesda MD, please come and have dinner with us. A flyer with all the information can be found on our web site (link below). We are looking for RSVPs by January 10 for our buffet at Dave and Buster's in White Flint Mall.
Agenda: 5:00-6:00 social hour with cash bar - 6:00-7:00 Dinner
7:00-8:00 Seminar - 8:00-8:30 Business Meeting.
Please RSVP to meetings-at-csmicroscopy.org so that we can have a headcount.
Cost: $25, cash/check at the door www.csmicroscopy.org
Happy New Year Chris
Christine A. Brantner, PhD [C] NIH NHLBI Electron Microscopy Core Facility National Institutes of Health 14 Service Road West Building 14E Room 104 MSC5570 Bethesda, MD 20892-5570
The notes quite easily on calculating the CCD temperature (dated 9th Nov 2011):
Step 1. Take two gain normalized images at 0.1 sec and 5.1 sec (blanked beam) and measure the average count rates per pixel (I_0.1 & I_5.1).
Step 2. Calculate the following quotient, q:
q = 10.037 * ln ((I_5.1 - I_0.1)/65.11424)
Where ln is the natural logarithm.
Step 3. The CCD temperature, T, in Kelvin is given by:
T = 20.0*(25 - q)
Example: I measured a difference in count rate of I_5.1 - I_0.1 = 209.9 counts per pixel, giving me a quotient of 11.748 and temperature of 265K (-8C).
I tried this several times to get some consistency: 217.7 counts =} -15.3C, etc.
I will try to find out exactly where these figures come from and update you if needed.
I hope this helps.
Yours, Jon
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A Healthy and Prosperous New Year to you, and the entire list.
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Best Regards, Darrell
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*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
realname - Liz Smith Email - elizabeth.smith.ctr-at-dha.mil ORGANIZATION - Defense Health Agency EDUCATION - Graduate College LOCATION - Falls Church, VA, USA SUBJECT_OF_QUESTION - Microscopy Finding - Definition QUESTION - To Whom It May Concern,
Microscopy Finding is a named, undefined attribute within Microbe Identification the Federal Health Information Model. Would you be able to help us with an authoritative definition for this term.
Respectfully, Liz Smith.
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Email: asw-at-sonnax.com Name: Amy Wall
Organization: Sonnax Industries
Title-Subject: [Filtered] Connect Nikon Epiphot 300 to digital camera
Message: Does anyone have experience connecting a Nikon Epiphot 300 metallograph to a digital camera? We are looking at a used Epiphot 300 and want to take pictures with a digital camera. The info I see says its simple to connect a digital camera to a side port but I find no further info on this "simple" connection. Thank you!
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Colleagues, I hope everyone had a relaxing break. I am about to embark on a fairly substantial project to characterize plant cell wall structure with immunogold labeling and imaging in FESEM. I have a few questions before investing in reagents.
I like the idea of using secondaries conjugated to ~1 nm gold followed by enhancement. How well does this work in practice? For my application I am not worried about 'too much' labeling and in fact I would like to see as much of the epitope as is present in the sample. I gather that there are both silver as well as gold enhancers. Anyone compare them? How does the 'shelf life' compare between a 10 nm- and a 1 nm- conjugate?
I will be labeling the surface of sectioned plant stems, so I am not worried about penetration through the sample. But I do wonder whether plant tissues / cell walls interact strangely with enhancement regents. Any experience?
Finally, and in general, I would appreciate comments on recent (last few years?) experiences with particular products/vendors.
Many many thanks, And those in the northern hemisphere stay warm!
Tobias -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Professor /_ / __ /__ \ \ \__ Biology Department / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / ___ / \ \__/ \ ____ Amherst, MA, 01003 www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 6, 18 -- From baskin-at-bio.umass.edu Tue Jan 7 20:35:57 2014 6, 18 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s082Zu24023733 6, 18 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jan 2014 20:35:56 -0600 6, 18 -- Received: from [10.0.1.2] (ip-159.250.64.67.dsl.crocker.net [159.250.64.67]) 6, 18 -- (authenticated bits=0) 6, 18 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id s082ZrYr019831 6, 18 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 18 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jan 2014 21:35:55 -0500 (EST) 6, 18 -- Mime-Version: 1.0 6, 18 -- Message-Id: {p0624057bcef2668fc923-at-[10.0.1.2]} 6, 18 -- Date: Tue, 7 Jan 2014 21:35:52 -0500 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 6, 18 -- Subject: immunogold for T or SEM 6, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 18 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Tue, 07 Jan 2014 21:35:55 -0500 (EST) 6, 18 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
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Organization: Sofia University, Faculty of Physics
Title-Subject: [Filtered] SEM Hitachi S570 documentation
Message: We have in the lab a Hitachi S570 SEM and we want to upgrade it. I am looking for the schematic documentation of the microscope column. I need to know the exact dimensions and separation distances in the column.
Can anyone help me to find it?
Thank you in advance.
G. Tsutsumanova
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I'm looking for TEM grid, 3.05 mm diameter, 200 mesh, carbon coated (about 200 A carbon thick) absolutely without impurity of Zn and Ni because I have to investigate (by EDS-TEM) on synthetic particles containing either Zn as Ni.
I already tried the Au, Cu, plastic grids and all contain some amount of at least one of these elements. I verified that the grids sold as Cu-grid contain either Ni as Zn. Do you have info on carbon coated grids with the characteristics I need?
I hope you can help me because I don't find a solution for this problem. I thank you a lot.
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Title-Subject: [Filtered] TEM sample preparation for Yeast and Fungi.
Message: Dear Listeners, How to prepare the yeast and fungi sample for TEM. Is there any other resin used instead of araldite.?
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==============================Original Headers============================== 14, 29 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 9 07:19:41 2014 14, 29 -- Received: from znl.com ([206.69.208.20]) 14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s09DJfD0016672 14, 29 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 07:19:41 -0600 14, 29 -- Received: from localhost (localhost [127.0.0.1]) 14, 29 -- by znl.com (Postfix) with ESMTP id D497F6A4B75 14, 29 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 07:19:41 -0600 (CST) 14, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 29 -- Received: from znl.com ([127.0.0.1]) 14, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 29 -- with ESMTP id YbKZRxJdGvVM for {microscopy-at-microscopy.com} ; 14, 29 -- Thu, 9 Jan 2014 07:19:40 -0600 (CST) 14, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 29 -- by znl.com (Postfix) with ESMTPA id BCC596A4B65 14, 29 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 07:19:40 -0600 (CST) 14, 29 -- Message-ID: {52CEA1EC.3070402-at-microscopy.com} 14, 29 -- Date: Thu, 09 Jan 2014 07:19:40 -0600 14, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 14, 29 -- MIME-Version: 1.0 14, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 29 -- Subject: viaWWW:TEM sample preparation for Yeast and Fungi.,,Message: Dear 14, 29 -- Listeners, 14, 29 -- References: {201401090918.s099IQ89027272-at-ns.microscopy.com} 14, 29 -- In-Reply-To: {201401090918.s099IQ89027272-at-ns.microscopy.com} 14, 29 -- X-Forwarded-Message-Id: {201401090918.s099IQ89027272-at-ns.microscopy.com} 14, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would be surprised if the stray EDX signals were coming from your grids. They are generally quite pure (at least at the TEM EDX level). TEM EDX detection limits will be a few tenths of an at% and grid impurities will be more in the ppm level. Also, the fact that you are seeing stray signal from many different grids suggests that the grids may not be the source. The only time i've ever seen impurities by EDX was a silicone contaminated C film where the diffusion pump oil backstreamed into the chamber during the film preparation. That batch of films showed huge Si contamination.
So, where is the stray signal coming from? I would suspect that you are seeing fluorescence from hard x-rays (bremsstrahlung) generated when the beam hits the C2 aperture. The bremsstrahlung will cause areas away from the sample in the sample chamber to fluoresce. This can be tested by putting the beam through a hole in the sample (called a "hole count"). In this case you should have no EDX signal at all. If you do see something, it is likely caused by the bremsstrahlung generated fluorescence.
Good luck, Henk
At 1/9/2014 5:57 AM, elena.belluso-at-unito.it wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Dear all, } } I'm looking for TEM grid, 3.05 mm diameter, 200 mesh, carbon coated } (about } 200 A carbon thick) absolutely without impurity of Zn and Ni because I } have } to investigate (by EDS-TEM) on synthetic particles containing either Zn } as } Ni. } } I already tried the Au, Cu, plastic grids and all contain some amount } of at } least one of these elements. I verified that the grids sold as Cu-grid } contain either Ni as Zn. Do you have info on carbon coated grids with } the } characteristics I need? } } I hope you can help me because I don't find a solution for this } problem. I } thank you a lot. } } Elena Belluso } } ------------------------------------------------------------------------------------ } } Elena BELLUSO } } Associate Professor in Environmental Mineralogy } } Ph.D. Mineralogy and Crystallography } } Dip. di Scienze della Terra, Universita' degli Studi di Torino } } Via Valperga Caluso n. 35, 10125 TORINO - ITALIA } } tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 } } e-mail: elena.belluso-at-unito.it [1] } } ------------------------------------------------------------------------------------ } } Elena BELLUSO } } Associate Professor in Environmental Mineralogy } } Ph.D. Mineralogy and Crystallography } } Dip. di Scienze della Terra, Universita' degli Studi di Torino } } Via Valperga Caluso n. 35, 10125 TORINO - ITALIA } } tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 } } e-mail: elena.belluso-at-unito.it [2] } } ------------------------------------------------------------------------------------ } } _"I've... seen things you people wouldn't believe. _ } } _Attack ships on fire off the shoulder of Orion. _ } } _I watched C-beams... glitter in the dark near the Tanhauser Gate. _ } } _All those... moments will be lost... in time..., _ } } _like... tears... in... rain." _ } } _Blade Runner_ } } } Links: } ------ } [1] mailto:elena.belluso-at-unito.it } [2] mailto:elena.belluso-at-unito.it } } ==============================Original Headers============================== } 32, 31 -- From elena.belluso-at-unito.it Thu Jan 9 04:55:48 2014 } 32, 31 -- Received: from mail-out2.unito.it (mail-out2.unito.it [130.192.119.63]) } 32, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s09Atlvh028560 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 04:55:48 -0600 } 32, 31 -- Received: (from root-at-localhost) } 32, 31 -- by mail-out2.unito.it (8.14.4/8.13.4/Debian-3sarge1) id s09AtlKI011223 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 11:55:47 +0100 } 32, 31 -- Received: from webmail.unito.it (webmail-balancer.unito.it [130.192.119.33]) } 32, 31 -- (authenticated bits=0) } 32, 31 -- by mail-out2.unito.it (8.14.4/8.13.4/Debian-3sarge1) with ESMTP id s09AtWNC010400 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 11:55:35 +0100 } 32, 31 -- X-Icontrol: Sent by Inrete Icontrol } 32, 31 -- MIME-Version: 1.0 } 32, 31 -- Content-Type: text/plain; charset=UTF-8; } 32, 31 -- format=flowed } 32, 31 -- Content-Transfer-Encoding: 7bit } 32, 31 -- Date: Thu, 09 Jan 2014 11:55:32 +0100 } 32, 31 -- From: Elena Belluso {elena.belluso-at-unito.it} } 32, 31 -- To: {Microscopy-at-microscopy.com} } 32, 31 -- Subject: TEM grid without Zn and Ni } 32, 31 -- Disposition-Notification-To: Elena Belluso {elena.belluso-at-unito.it} } 32, 31 -- Message-ID: {f6d345d3a6d5445bdd859ad77475a0a4-at-unito.it} } 32, 31 -- X-Sender: elena.belluso-at-unito.it } 32, 31 -- User-Agent: Roundcube Webmail/0.6 } 32, 31 -- X-From-User: ebelluso-at-unito.it } 32, 31 -- X-IControl-Milter-Checked: yes (IControlServer: mail-out2 SrcIpType: UnTrusted SrcIpHeaderType: Undefined Authenticated: yes) } 32, 31 -- X-IControl-Milter-SPF-Checked: yes (IControlServer: mail-out2 HeloSPFType: none FromSPFType: none HeloHeaderSPFType: Undefined FromHeaderSPFType: none) } 32, 31 -- X-IControl-Milter-MD5SUM: a74f735de91a38b9d0ae2313a42824ef } 32, 31 -- X-Inrete-Amavisjob-Virus-Scanned: PDAmail Multiple Antivirus with ClamAv } 32, 31 -- X-Inrete-Amavisjob-Service-Runned: 6 (s09AtWNC010400) } 32, 31 -- X-Inrete-Amavisjob-Service-Disabled: No Service disabled (s09AtWNC010400) } ==============================End of - Headers============================== }
-- Untitled Document
Hendrik O. Colijn *C*enter for *E*lectron *M*icroscopy & *A*nalysi*S* 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
Hi Ravi, You can use the protocol from Janice Griffith et al. in Journal of Electron Microscopy 60(3); 211-216 (2011). This is for IEM but you can also use it to prepare the sample for plastic embedding.
Groeten, Hans The Netherlands Cancer Institute. Amsterdam.
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: donderdag 9 januari 2014 14:24 To: Hans Janssen
X-from: ravi.thakkar369-at-gmail.com ()
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Title-Subject: [Filtered] TEM sample preparation for Yeast and Fungi.
Message: Dear Listeners, How to prepare the yeast and fungi sample for TEM. Is there any other resin used instead of araldite.?
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Happy new year to you all! I would appreciate your comments on the new non-radioactive Uranyl Acetate replacement Stain (a substitute for uranyl acetate). How do you like this product? What's your staining time? I know this was discussed not long ago so email off list if you prefer. thanks, Beth
==============================Original Headers============================== 2, 21 -- From beth-at-plantbio.uga.edu Thu Jan 9 09:08:48 2014 2, 21 -- Received: from dogwood.plantbio.uga.edu (pbmail.plantbio.uga.edu [128.192.26.3]) 2, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s09F8mco009632 2, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 09:08:48 -0600 2, 21 -- X-Footer: cGxhbnRiaW8udWdhLmVkdQ== 2, 21 -- Received: from [128.192.145.94] ([128.192.145.94]) 2, 21 -- (authenticated user beth-at-plantbio.uga.edu) 2, 21 -- by dogwood.plantbio.uga.edu 2, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 2, 21 -- for microscopy-at-microscopy.com; 2, 21 -- Thu, 9 Jan 2014 10:08:44 -0500 2, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 2, 21 -- Content-Type: text/plain; charset=us-ascii 2, 21 -- Subject: Uranyl Acetate replacement Stain 2, 21 -- Date: Thu, 9 Jan 2014 10:08:43 -0500 2, 21 -- Message-Id: {8CB76706-C8B1-46FD-B773-5C9FC3F921B1-at-plantbio.uga.edu} 2, 21 -- To: microscopy microscopy {microscopy-at-microscopy.com} 2, 21 -- Mime-Version: 1.0 (Apple Message framework v1085) 2, 21 -- X-Mailer: Apple Mail (2.1085) 2, 21 -- Content-Transfer-Encoding: 8bit 2, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s09F8mco009632 ==============================End of - Headers==============================
You may wish to consider using SiN windows mounted on thin Si wafers. These are available at most EM Supply houses. I have used these for supporting small particles and have successfully done XEDS on these for years. You will, of course, detect Si, N, and some O. I have never seen Zn or Ni with these SiN films in instruments here at the ANL EMCenter.
Look for Si wafers ~ 100 microns thick, rather than the thicker (200 -300 micron) ones. The SiN windows range from 10-100 nm, obviously the thinner the support window the better, but admittedly the 10nm thick windows are fragile. Just be careful, or start with thicker windows and work your way down.
Finally, be cognizant of which side you deposit your particles and also which side is facing your XEDS detector, in order that you do not get any shadowing effects due to the penumbra of the stage and/or the chemically etched Si surface.
Cheers,
Nestor Your Friendly Neighborhood SysOp
On 1/9/14 4:55 AM, elena.belluso-at-unito.it wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Dear all, } } I'm looking for TEM grid, 3.05 mm diameter, 200 mesh, carbon coated } (about } 200 A carbon thick) absolutely without impurity of Zn and Ni because I } have } to investigate (by EDS-TEM) on synthetic particles containing either Zn } as } Ni. } } I already tried the Au, Cu, plastic grids and all contain some amount } of at } least one of these elements. I verified that the grids sold as Cu-grid } contain either Ni as Zn. Do you have info on carbon coated grids with } the } characteristics I need? } } I hope you can help me because I don't find a solution for this } problem. I } thank you a lot. } } Elena Belluso } } ------------------------------------------------------------------------------------ } } Elena BELLUSO } } Associate Professor in Environmental Mineralogy } } Ph.D. Mineralogy and Crystallography } } Dip. di Scienze della Terra, Universita' degli Studi di Torino } } Via Valperga Caluso n. 35, 10125 TORINO - ITALIA } } tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 } } e-mail: elena.belluso-at-unito.it [1] } } ------------------------------------------------------------------------------------ } } Elena BELLUSO } } Associate Professor in Environmental Mineralogy } } Ph.D. Mineralogy and Crystallography } } Dip. di Scienze della Terra, Universita' degli Studi di Torino } } Via Valperga Caluso n. 35, 10125 TORINO - ITALIA } } tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 } } e-mail: elena.belluso-at-unito.it [2] } } ------------------------------------------------------------------------------------ } } _"I've... seen things you people wouldn't believe. _ } } _Attack ships on fire off the shoulder of Orion. _ } } _I watched C-beams... glitter in the dark near the Tanhauser Gate. _ } } _All those... moments will be lost... in time..., _ } } _like... tears... in... rain." _ } } _Blade Runner_ } } } Links: } ------ } [1] mailto:elena.belluso-at-unito.it } [2] mailto:elena.belluso-at-unito.it } } ==============================Original Headers============================== } 32, 31 -- From elena.belluso-at-unito.it Thu Jan 9 04:55:48 2014 } 32, 31 -- Received: from mail-out2.unito.it (mail-out2.unito.it [130.192.119.63]) } 32, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s09Atlvh028560 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 04:55:48 -0600 } 32, 31 -- Received: (from root-at-localhost) } 32, 31 -- by mail-out2.unito.it (8.14.4/8.13.4/Debian-3sarge1) id s09AtlKI011223 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 11:55:47 +0100 } 32, 31 -- Received: from webmail.unito.it (webmail-balancer.unito.it [130.192.119.33]) } 32, 31 -- (authenticated bits=0) } 32, 31 -- by mail-out2.unito.it (8.14.4/8.13.4/Debian-3sarge1) with ESMTP id s09AtWNC010400 } 32, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 11:55:35 +0100 } 32, 31 -- X-Icontrol: Sent by Inrete Icontrol } 32, 31 -- MIME-Version: 1.0 } 32, 31 -- Content-Type: text/plain; charset=UTF-8; } 32, 31 -- format=flowed } 32, 31 -- Content-Transfer-Encoding: 7bit } 32, 31 -- Date: Thu, 09 Jan 2014 11:55:32 +0100 } 32, 31 -- From: Elena Belluso {elena.belluso-at-unito.it} } 32, 31 -- To: {Microscopy-at-microscopy.com} } 32, 31 -- Subject: TEM grid without Zn and Ni } 32, 31 -- Disposition-Notification-To: Elena Belluso {elena.belluso-at-unito.it} } 32, 31 -- Message-ID: {f6d345d3a6d5445bdd859ad77475a0a4-at-unito.it} } 32, 31 -- X-Sender: elena.belluso-at-unito.it } 32, 31 -- User-Agent: Roundcube Webmail/0.6 } 32, 31 -- X-From-User: ebelluso-at-unito.it } 32, 31 -- X-IControl-Milter-Checked: yes (IControlServer: mail-out2 SrcIpType: UnTrusted SrcIpHeaderType: Undefined Authenticated: yes) } 32, 31 -- X-IControl-Milter-SPF-Checked: yes (IControlServer: mail-out2 HeloSPFType: none FromSPFType: none HeloHeaderSPFType: Undefined FromHeaderSPFType: none) } 32, 31 -- X-IControl-Milter-MD5SUM: a74f735de91a38b9d0ae2313a42824ef } 32, 31 -- X-Inrete-Amavisjob-Virus-Scanned: PDAmail Multiple Antivirus with ClamAv } 32, 31 -- X-Inrete-Amavisjob-Service-Runned: 6 (s09AtWNC010400) } 32, 31 -- X-Inrete-Amavisjob-Service-Disabled: No Service disabled (s09AtWNC010400) } ==============================End of - Headers============================== }
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Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Bldg 212 / A-143 9700 S. Cass Ave Argonne, Illinois 60439 USA
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Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
For yeast cells, cryo-fixation works much better than conventional chemical fixation. You can either use self-pressurized rapid freezing method or high pressure freezing method. The advantage of self pressurized rapid freezing is that you do not need specialized equipment and it is very easy to do. I routinely use this method for processing yeast cells. Following cryo-fixation, you will need to do cryo-substitution and eventually embed yeast cells in resin.
You can find more information about self-pressurized rapid freezing in the manuscript shown below:
Leunissen JL, Yi H (2009) Self-pressurized rapid freezing (SPRF): a novel cryofixation method for specimen preparation in electron microscopy. J Microsc (Oxford) 235:2535.
Hong
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Title-Subject: [Filtered] Sample preparation for TEM
Message: Hi Everybody
How can I prepare a sample of ZnO thin film deposited on glass substrate by RF Magnetron sputtering, so i can analyze it in TEM
Is there any published method or somebody knows an easy way?
Thanks
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 9 18:48:42 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0A0mg7t011332 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:42 -0600 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id 994B46A50A4 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:42 -0600 (CST) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id JRtDDcrJAwqU for {microscopy-at-microscopy.com} ; 17, 28 -- Thu, 9 Jan 2014 18:48:41 -0600 (CST) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id B917B6A5097 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:41 -0600 (CST) 17, 28 -- Message-ID: {52CF4369.2080800-at-microscopy.com} 17, 28 -- Date: Thu, 09 Jan 2014 18:48:41 -0600 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Sample preparation ZnO for TEM 17, 28 -- References: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: mmcgough-at-histochemicalsociety.org Name: Meg McGough
Organization: Histochemical Society
Title-Subject: [Filtered] MBL Immunohistochemistry and Microscopy Course
Message: Registration is still open for the 2014 Immunohistochemistry and Microscopy Course (IHCM) at the Marine Biological Laboratory. Please alert your students, postdocs and colleagues to this opportunity to attend one of MBL's Special Topics course on Immunohistochemistry and Microscopy. Registration closes on Tuesday, January 14! Course dates are March 15-20, 2014.
The Immunohistochemistry and Microscopy (IHCM) course is four full days and evenings (11 hours daily) of lecture and laboratory sessions with experts in the field of immunohistochemistry (IHC) and microscopy. The IHCM course goal is to provide participants in-depth theory of and extensive hands-on experience with immunohistochemistry (IHC) techniques as well as theory and hands-on experience with a broad range of microscopic imaging techniques. The course emphasizes hands-on laboratory time and small breakout discussions with faculty and staff.
This course is appropriate for undergraduate and graduate students, laboratory technicians, postdoctoral students, new and established faculty/clinicians seeking to expand their techniques and knowledge of IHC and microscopy. It is appropriate for beginning scientists and those with more advanced skills. Participants will be grouped appropriately. Registration is limited to thirty.
Completed application forms and required supporting documentation must be received by January 14, 2014.
FASEB Marc Awards and HCS Travel Awards are available to support attendance at the course: http://histochemicalsociety.org/Awards.aspx
For further information about the course: http://hermes.mbl.edu/education/courses/special_topics/ihcm.html
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Email: info-at-nesmicroscopy.org Name: New England Society for Microscopy
Organization: New England Society for Microscopy
Title-Subject: [Filtered] Save the Date: NESM's Annual Spring Meeting -at- Cabot Corporation!
Message: Dear Fellow Microscopists,
Remember to save the date for the New England Society for MicroscopyÂ’s annual Spring Meeting on Tuesday, February 25th at Cabot Corporation! The meeting will consist of a buffet dinner and two technical talks. More information to follow when registration opens up in the coming weeks. Keep up with NESM by visiting our website (www.nesmicroscopy.org) and by following us on Facebook (http://www.facebook.com/NESMicroscopy) and on twitter (http://www.twitter.com/#!/NESMicroscopy).
We hope to see you in February!
Cheers, NESM Board
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I'd like to explore lab management software that includes calendar, user approval, account management, invoicing etc. I have found iLab, OCF, PPMS and Faces. Are their other products? I'd like to talk (off line) with those that use these products.
Disclaimer. I don't have a financial relationship with any of the vendors that distribute the products I mention in this message.
Thank you.
Owen
Owen P. Mills Director, Applied Chemical and Morphological Analysis Laboratory ACMAL Materials Science & Engineering Michigan Technological University 1400 Townsend Drive Houghton, MI 49931 PH 906-369-1875 FAX 906-487-2934 opmills-at-mtu.edu
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The easiest and fastest way would be to deposit your coating on a #0 or #1 glass cover plate and use a slightly modified version of the small angle cleavage technique. When you start scribing with the mini scribes, some pieces will have a small angle and will be good specimens when you mount them on the grid vertically. For ZnO, the transition region from equiaxed growth to columnar is weak and you often get a jog in the cross section of the film with the columnar structure back further from the tip. It just means that you have to screen more samples in the microscope. Get your hands on the MRS vol 480 book for Sample Prep IV and look at John McCaffrey and my paper.
The Small Angle Cleavage Technique: An Update, S. D. Walck and J. P. McCaffrey, Proceedings of the Materials Research Society, Workshop on Specimen Preparation for Transmission Electron Microscopy of Materials IV, eds. Ron M. Anderson and Scott D. Walck, Vol 480, Pittsburgh, (1997).
I think that we talk about ZnO results in this paper, but I'm not 100% positive: The Small Angle Cleavage Technique Applied to Coatings and Thin Films, S. D. Walck and J. P. McCaffrey, Thin Solid Films, 308-309, pp. 399-405, 1997.
- -Scott
Scott D. Walck, Ph.D. Sr. Scientist Bowhead Science & Technology U.S. Army Research Laboratory RDRL-WMM-C 4600 Deer Creek Loop APG, MD 21005-5069 USA
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Title-Subject: [Filtered] Sample preparation for TEM
Message: Hi Everybody
How can I prepare a sample of ZnO thin film deposited on glass substrate by RF Magnetron sputtering, so i can analyze it in TEM
Is there any published method or somebody knows an easy way?
Thanks
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 9 18:48:42 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0A0mg7t011332 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:42 -0600 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id 994B46A50A4 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:42 -0600 (CST) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id JRtDDcrJAwqU for {microscopy-at-microscopy.com} ; 17, 28 -- Thu, 9 Jan 2014 18:48:41 -0600 (CST) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id B917B6A5097 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jan 2014 18:48:41 -0600 (CST) 17, 28 -- Message-ID: {52CF4369.2080800-at-microscopy.com} 17, 28 -- Date: Thu, 09 Jan 2014 18:48:41 -0600 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Sample preparation ZnO for TEM 17, 28 -- References: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201401100022.s0A0M6bZ010863-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 31, 36 -- From s.walck-at-comcast.net Sat Jan 11 13:36:11 2014 31, 36 -- Received: from qmta05.westchester.pa.mail.comcast.net (qmta05.westchester.pa.mail.comcast.net [76.96.62.48]) 31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0BJaAUs024374 31, 36 -- for {microscopy-at-microscopy.com} ; Sat, 11 Jan 2014 13:36:11 -0600 31, 36 -- Received: from omta06.westchester.pa.mail.comcast.net ([76.96.62.51]) 31, 36 -- by qmta05.westchester.pa.mail.comcast.net with comcast 31, 36 -- id Chcz1n00416LCl055jc8lA; Sat, 11 Jan 2014 19:36:08 +0000 31, 36 -- Received: from WalckPC ([IPv6:2601:a:80:4e1:64f7:a070:5cc2:96fa]) 31, 36 -- by omta06.westchester.pa.mail.comcast.net with comcast 31, 36 -- id Cjc81n00107uUYL3Sjc84A; Sat, 11 Jan 2014 19:36:08 +0000 31, 36 -- From: "Scott Walck" {S.Walck-at-comcast.net} 31, 36 -- To: {microscopy-at-microscopy.com} 31, 36 -- References: {201401100108.s0A18ano027725-at-ns.microscopy.com} 31, 36 -- In-Reply-To: {201401100108.s0A18ano027725-at-ns.microscopy.com} 31, 36 -- Subject: RE: [Microscopy] viaWWW:Sample preparation ZnO for TEM 31, 36 -- Date: Sat, 11 Jan 2014 14:36:05 -0500 31, 36 -- Message-ID: {017c01cf0f04$5e6c37b0$1b44a710$-at-comcast.net} 31, 36 -- MIME-Version: 1.0 31, 36 -- Content-Type: text/plain; 31, 36 -- charset="iso-8859-1" 31, 36 -- X-Mailer: Microsoft Outlook 14.0 31, 36 -- Thread-Index: AQItE8gNsZ350rWjfhi9XF8u27v9zpnD3uGA 31, 36 -- Content-Language: en-us 31, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 31, 36 -- s=q20121106; t=1389468968; 31, 36 -- bh=g+KGtoG+VLcYPXhWep2BmjCaioLG4mb5FckijAf+PuQ=; 31, 36 -- h=Received:Received:From:To:Subject:Date:Message-ID:MIME-Version: 31, 36 -- Content-Type; 31, 36 -- b=Aa+CaMZym4S5EcahM3ZFeVMcUvIUMQ0Aj9gRQnuAeGU8CBNAr3qOKu153BNgM+/AR 31, 36 -- 0ob3GiSl+c4MlNNxciREAfqA2LXPOz9eFZF4gPiS/+VU2Yt4YMOWz/6ymxp3hfvqtP 31, 36 -- uo2QAhOdON6FqBcr6UF2pPCN1zW61jmVFOZcFYu3L45zEQswTCS22dSyhI1wOhtDDl 31, 36 -- FZA5bifxc3Vj2zEx5UrXTSjxNSwiUaq93xVEndiOlrSUJ1A7MAec7Yp2DgoXUUnP9J 31, 36 -- wM+PdStC5CALm6eOXWMDIfArfLD5BkODia0zamfl8FY+uViXlQdv3ZQ2/lUgww1jtF 31, 36 -- tARefGKmDuhrQ== 31, 36 -- Content-Transfer-Encoding: 8bit 31, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0BJaAUs024374 ==============================End of - Headers==============================
We have a couple of Hitachi CuteVac VR16L-K roughing pumps that need new vanes and oil seals. Unfortunately, nobody seems to sell parts for these. A couple of people have suggested that the parts are interchangeable with another manufacturer, (Alcatel?) but nobody has been able to provide specific part #s for the vanes or anything else.
Anyone rebuilt one of these before and remember which vanes and seals to use?
Thanks, Bradford Ross
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-6996
==============================Original Headers============================== 13, 31 -- From bradford.ross-at-botany.ubc.ca Mon Jan 13 13:28:40 2014 13, 31 -- Received: from vmaprod3.mail-relay.ubc.ca (vmaprod3.mail-relay.ubc.ca [142.103.117.136]) 13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0DJSeBU001075 13, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 13:28:40 -0600 13, 31 -- Received: from vmaprod3.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 13, 31 -- by localhost (Email Security Appliance) with SMTP id DAA341CB5AB_2D43E67B 13, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 19:28:39 +0000 (GMT) 13, 31 -- Received: from smtp.mail.ubc.ca (s-itsv-hub04p.ead.ubc.ca [137.82.151.86]) 13, 31 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 13, 31 -- (Client CN "rpc.mail.ubc.ca", Issuer "Entrust Certification Authority - L1C" (verified OK)) 13, 31 -- by vmaprod3.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id A5B751CB5A0_2D43E67F 13, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 19:28:39 +0000 (GMT) 13, 31 -- Received: from S-ITSV-MBX05P.ead.ubc.ca ([169.254.12.43]) by 13, 31 -- s-itsv-hub04p.ead.ubc.ca ([137.82.151.86]) with mapi id 14.03.0174.001; Mon, 13, 31 -- 13 Jan 2014 11:28:39 -0800 13, 31 -- From: "Ross, Bradford" {bradford.ross-at-botany.ubc.ca} 13, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 13, 31 -- Subject: Hitachi RP Rebuild 13, 31 -- Thread-Topic: Hitachi RP Rebuild 13, 31 -- Thread-Index: Ac8QlWu5a75tm1e1Qe+heqfjHsrttw== 13, 31 -- Date: Mon, 13 Jan 2014 19:28:38 +0000 13, 31 -- Message-ID: {B48A2601A4664A4AAF5A3D2F0E48280101527164B5-at-s-itsv-mbx05p.ead.ubc.ca} 13, 31 -- Accept-Language: en-US, en-CA 13, 31 -- Content-Language: en-US 13, 31 -- X-MS-Has-Attach: 13, 31 -- X-MS-TNEF-Correlator: 13, 31 -- x-originating-ip: [137.82.85.253] 13, 31 -- Content-Type: text/plain; charset="us-ascii" 13, 31 -- MIME-Version: 1.0 13, 31 -- Content-Transfer-Encoding: 8bit 13, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0DJSeBU001075 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Registration Reminder: FIB SEM Workshop, Feb 27, 2014 and Johns Hopkins APL
Message: Call for Papers & Registration Reminder
7th Annual FIB SEM Workshop
Thursday, February 27, 2014
Kossiakoff Center
Johns Hopkins Applied Physics Laboratory
Laurel, MD
2014 FIB SEM Workshop will be held at the Kossiakoff Center at Johns Hopkins Applied Physics Laboratory (APL). We will have a full day of presentations and posters by FIB users and vendors highlighting interesting new FIB applications and the latest technology. And, as always, there will be plenty of opportunities for informal discussion of new techniques and applications as well as getting (re)connected with your fellow FIBers.
If you would like to present a paper or poster, please submit an abstract to the organizers. Starting this year, we are requiring an abstract submission from all presenters (user & vendor contributions). All FIB related topics such as new and novel application areas for FIB, advances in FIB and FIB related instrumentation and techniques, image and data processing techniques for FIB data are all welcome.
· Abstract Deadline: January 15, 2014
Abstracts should be 250 words or less in length. Email abstracts to fibsem2014-at-fibsem.net.
Abstract Notification: February 1, 2014
· Registration Deadline: February 3, 2014 - Register!
· Travel Info
Please check our website (www.fibsem.org) for additional information about the meeting. There is no fee to attend the meeting, and breakfast and lunch will be provided! There will be a Happy Hour following the meeting, so donÂ’t forget to sign up for it when you register.
Remember, you don't have to be a local user to attend! We look forward to seeing you at APL!
Organizers:
Nabil Bassim, nabil.bassim-at-nrl.navy.mil
Ken Livi, klivi-at-jhu.edu
Joan Hoffmann, joan.hoffmann-at-jhuapl.edu
Keana Scott, keana.scott-at-nist.gov
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==============================Original Headers============================== 38, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 13 17:55:29 2014 38, 28 -- Received: from znl.com ([206.69.208.20]) 38, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0DNtTQb008982 38, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:55:29 -0600 38, 28 -- Received: from localhost (localhost [127.0.0.1]) 38, 28 -- by znl.com (Postfix) with ESMTP id 16D9C6A7E2A 38, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:55:29 -0600 (CST) 38, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 38, 28 -- Received: from znl.com ([127.0.0.1]) 38, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 38, 28 -- with ESMTP id MnL16QTMLXJ3 for {microscopy-at-microscopy.com} ; 38, 28 -- Mon, 13 Jan 2014 17:55:22 -0600 (CST) 38, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 38, 28 -- by znl.com (Postfix) with ESMTPA id 28AF76A7E1D 38, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:55:22 -0600 (CST) 38, 28 -- Message-ID: {52D47CE9.50205-at-microscopy.com} 38, 28 -- Date: Mon, 13 Jan 2014 17:55:21 -0600 38, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 38, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 38, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 38, 28 -- MIME-Version: 1.0 38, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 38, 28 -- Subject: viaWWW:FIB SEM Workshop, Feb 27, 2014 -at- Johns Hopkins APL 38, 28 -- References: {201401131623.s0DGNNBm030039-at-ns.microscopy.com} 38, 28 -- In-Reply-To: {201401131623.s0DGNNBm030039-at-ns.microscopy.com} 38, 28 -- X-Forwarded-Message-Id: {201401131623.s0DGNNBm030039-at-ns.microscopy.com} 38, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 38, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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You might want to obtain books written by Rick Giberson, since he is one of the inventors and pioneers for the microwave tissue processor. The microwave processor with vacuumm is one of the best inventions of the last 50 years. By using the microwave with vacuum you can now see organelles that you didn't know were there before. It is almost indispensible for lung or other tissues where you have lots of voids and spaces. We routinely use the technology on mouse and drosophila tissue. In the past I have used it on avian, reptile, and plant tissue also with excellent results. I am sure that if you call Ted Pella, Rick or spomeone would be happy to help you convert any protocols you may have.
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 13 17:56:28 2014 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0DNuSH5009940 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:56:28 -0600 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id 196236A7E39 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:56:28 -0600 (CST) 15, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id dLxU312YhYVA for {microscopy-at-microscopy.com} ; 15, 28 -- Mon, 13 Jan 2014 17:56:26 -0600 (CST) 15, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id D24936A7E2C 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:56:26 -0600 (CST) 15, 28 -- Message-ID: {52D47D2A.8070800-at-microscopy.com} 15, 28 -- Date: Mon, 13 Jan 2014 17:56:26 -0600 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:microwave processing 15, 28 -- References: {201401131943.s0DJh1Eo016083-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201401131943.s0DJh1Eo016083-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201401131943.s0DJh1Eo016083-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: mmcgough-at-histochemicalsociety.org Name: Meg McGough
Message: The application deadline for the 2014 Immunohistochemistry and Microscopy Course (IHCM) at the Marine Biological Laboratory has been extended until January 21. Course dates are March 15-20, 2014.
The Immunohistochemistry and Microscopy (IHCM) course is four full days and evenings (11 hours daily) of lecture and laboratory sessions with experts in the field of immunohistochemistry (IHC) and microscopy. The IHCM course goal is to provide participants in-depth theory of and extensive hands-on experience with immunohistochemistry (IHC) techniques as well as theory and hands-on experience with a broad range of microscopic imaging techniques. The course emphasizes hands-on laboratory time and small breakout discussions with faculty and staff.
This course is appropriate for undergraduate and graduate students, laboratory technicians, postdoctoral students, new and established faculty/clinicians seeking to expand their techniques and knowledge of IHC and microscopy. It is appropriate for beginning scientists and those with more advanced skills. Participants will be grouped appropriately. Registration is limited to thirty.
Completed application forms and required supporting documentation must be received by January 21, 2014.
FASEB Marc Awards are available to support attendance at the course: http://www.faseb.org/MARC-and-Professional-Development/Travel-Awards/Scientific-Meetings-and-Conferences.aspx
For further information about the course: http://hermes.mbl.edu/education/courses/special_topics/ihcm.html
Thank you, Meg McGough The Histochemical Society
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jan 13 17:58:34 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0DNwX4O012968 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:58:33 -0600 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 5A70B6A7E4C 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:58:33 -0600 (CST) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id rYSA58GupOYN for {microscopy-at-microscopy.com} ; 16, 28 -- Mon, 13 Jan 2014 17:58:31 -0600 (CST) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 1ACAB6A7E3E 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2014 17:58:31 -0600 (CST) 16, 28 -- Message-ID: {52D47DA6.1050902-at-microscopy.com} 16, 28 -- Date: Mon, 13 Jan 2014 17:58:30 -0600 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:MBL Immunohistochemistry and Microscopy Course 16, 28 -- References: {201401131934.s0DJYILh006048-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201401131934.s0DJYILh006048-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201401131934.s0DJYILh006048-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Elena, Have you considered that the Zn/Ni could be coming from the TEM or sample holder (or even the EDS detector) rather than the grid itself. When you hit the sample with high energy elections, you generate a huge number of x-rays of all energies in addition to backscattered electrons. If you have any brass near your sample, you are almost guaranteed to get Cu and Zn in your EDS spectrum from non-local fluorescence. The Ni could be coming from SS near the sample or the TEM pole piece (do you also see Fe?). While TEM based EDS is a lot cleaner than it once was, you can never really be sure where the x-rays that enter your detector originate. Using low background holders and TEM hard x-ray apertures (if available) can help. Chapter 33.3 in Williams and Carter's TEM book details these issues and describes precautions to help reduce system and spurious x-ray artifacts.
If you have access to an EELS detector, you can run the experiments without worrying about fluorescence artifacts. You will only see the element if it is under the beam.
Hope this helps.
-Ray
Disclaimer: I work for Gatan which provides EELS and EDS equipment and software as well as low background TEM sample holders.
Ray D. Twesten Livermore, CA
-----Original Message----- X-from: elena.belluso-at-unito.it [mailto:elena.belluso-at-unito.it] Sent: Thursday, January 09, 2014 3:04 AM To: ray.twesten-at-sbcglobal.net
Dear all,
I'm looking for TEM grid, 3.05 mm diameter, 200 mesh, carbon coated (about 200 A carbon thick) absolutely without impurity of Zn and Ni because I have to investigate (by EDS-TEM) on synthetic particles containing either Zn as Ni.
I already tried the Au, Cu, plastic grids and all contain some amount of at least one of these elements. I verified that the grids sold as Cu-grid contain either Ni as Zn. Do you have info on carbon coated grids with the characteristics I need?
I hope you can help me because I don't find a solution for this problem. I thank you a lot.
For the purpose of orientation while viewing a specimen in TEM, I naively thought it might be useful to "color" a resin by adding electrodense grains (e.g.ferritin) and then, after appropriate cutting, to re-embed the block in the "colored" resin. In this way one or more side(s) of the specimen touching the cut can be readily recognizable in the thin sections under the TEM.
Does it make any sense what I am saying and (if yes) is there any experience on this?
April 8, 2014 marks the end of support from Microsoft for Windows XP. That means no more security updates to patch newly discovered security holes. This will leave any computers still running Windows XP vulnerable to attacks if they remain connected to the internet or if infected memory media are inserted into the computer. I am sure there are a lot of machines out there running Windows XP. I am curious to hear what approaches microscopy community members are taking to deal with this, as well as what the community of microscopy related hardware vendors are doing to address this situation. Thank you.
John Mardinly, ASU
==============================Original Headers============================== 4, 35 -- From John.Mardinly-at-asu.edu Wed Jan 15 15:12:14 2014 4, 35 -- Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0FLCEU1008827 4, 35 -- for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 15:12:14 -0600 4, 35 -- X-ASG-Debug-ID: 1389820333-0549c456250a750001-FOsErg 4, 35 -- Received: from exhubw01.asurite.ad.asu.edu (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnetw3.asu.edu with ESMTP id Lezl3NXq5dB4vpTh (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 14:12:13 -0700 (MST) 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 4, 35 -- X-ASG-Whitelist: Client 4, 35 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 4, 35 -- exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id 14.02.0309.002; 4, 35 -- Wed, 15 Jan 2014 14:12:12 -0700 4, 35 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 35 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 4, 35 -- Subject: Windows XP Support Ending April 8, 2014 4, 35 -- Thread-Topic: Windows XP Support Ending April 8, 2014 4, 35 -- X-ASG-Orig-Subj: Windows XP Support Ending April 8, 2014 4, 35 -- Thread-Index: Ac8SNnTksb2ozlmjTzO6+BJCz2aDAQ== 4, 35 -- Date: Wed, 15 Jan 2014 21:12:11 +0000 4, 35 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400B7A2850-at-exmbt05.asurite.ad.asu.edu} 4, 35 -- Accept-Language: en-US 4, 35 -- Content-Language: en-US 4, 35 -- X-MS-Has-Attach: 4, 35 -- X-MS-TNEF-Correlator: 4, 35 -- x-originating-ip: [129.219.103.4] 4, 35 -- Content-Type: text/plain; charset="us-ascii" 4, 35 -- MIME-Version: 1.0 4, 35 -- X-Barracuda-Connect: exhubw01.asurite.ad.asu.edu[129.219.4.199] 4, 35 -- X-Barracuda-Start-Time: 1389820333 4, 35 -- X-Barracuda-Encrypted: AES128-SHA 4, 35 -- X-Barracuda-URL: http://149.169.2.73:8000/cgi-mod/mark.cgi 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 35 -- X-Barracuda-BRTS-Status: 1 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0FLCEU1008827 ==============================End of - Headers==============================
At my place, we are putting a Win7 computer between the XPs and the corporate network. The Win7 will act as a server for the XPs. We have a few SEMs and a couple of other tools that cannot be upgraded to Win7. Unless, of course, we want to buy whole new tools!
} -----Original Message----- } From: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] } Sent: Wednesday, January 15, 2014 3:12 PM } To: Holdford, Becky } Subject: [Microscopy] Windows XP Support Ending April 8, 2014 } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To
==============================Original Headers============================== 3, 32 -- From r-holdford-at-ti.com Wed Jan 15 17:50:40 2014 3, 32 -- Received: from devils.ext.ti.com (devils.ext.ti.com [198.47.26.153]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0FNoexB030568 3, 32 -- for {microscopy-at-microscopy.com} ; Wed, 15 Jan 2014 17:50:40 -0600 3, 32 -- Received: from dflxv15.itg.ti.com ([128.247.5.124]) 3, 32 -- by devils.ext.ti.com (8.13.7/8.13.7) with ESMTP id s0FNobgP008756; 3, 32 -- Wed, 15 Jan 2014 17:50:38 -0600 3, 32 -- Received: from DFLE72.ent.ti.com (dfle72.ent.ti.com [128.247.5.109]) 3, 32 -- by dflxv15.itg.ti.com (8.14.3/8.13.8) with ESMTP id s0FNobmc010160; 3, 32 -- Wed, 15 Jan 2014 17:50:37 -0600 3, 32 -- Received: from DLEE08.ent.ti.com ([fe80::b57d:c966:76e5:eb71]) by 3, 32 -- DFLE72.ent.ti.com ([fe80::e5e2:bab6:34dc:a483%28]) with mapi id 3, 32 -- 14.02.0342.003; Wed, 15 Jan 2014 17:50:37 -0600 3, 32 -- From: "Holdford, Becky" {r-holdford-at-ti.com} 3, 32 -- To: "John.Mardinly-at-asu.edu" {John.Mardinly-at-asu.edu} 3, 32 -- CC: "MSA Listerver (microscopy-at-microscopy.com)" {microscopy-at-microscopy.com} 3, 32 -- Subject: RE: [Microscopy] Windows XP Support Ending April 8, 2014 3, 32 -- Thread-Topic: [Microscopy] Windows XP Support Ending April 8, 2014 3, 32 -- Thread-Index: AQHPEjZ+7S3V71p2l0enBlFa2Pign5qGdABA 3, 32 -- Date: Wed, 15 Jan 2014 23:50:36 +0000 3, 32 -- Message-ID: {9128BB8114AB41469F2BAA1EA3A4F85D237086B2-at-DLEE08.ent.ti.com} 3, 32 -- References: {201401152112.s0FLCOuv009012-at-ns.microscopy.com} 3, 32 -- In-Reply-To: {201401152112.s0FLCOuv009012-at-ns.microscopy.com} 3, 32 -- Accept-Language: en-US 3, 32 -- Content-Language: en-US 3, 32 -- X-MS-Has-Attach: 3, 32 -- X-MS-TNEF-Correlator: 3, 32 -- x-originating-ip: [128.247.5.50] 3, 32 -- Content-Type: text/plain; charset="us-ascii" 3, 32 -- MIME-Version: 1.0 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0FNoexB030568 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sergey-at-seas.ucla.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
The Southern California Society for Microscopy and Microanalysis (SCSMM) announce their Full-Day Symposium. The meeting will be held on Saturday, February 8th from 8:00 am at Calit2 Auditorium at the University of California, Irvine. A lunch and coffee breaks will be served.
Our symposium, dedicated to hybrid approaches in microscopy. Beside the fact that it poses significant challenges for researchers in both life and materials sciences, it continues the conversation about nanoscience, its role, and its future. We are honored to have Prof. C. Barry Carter from the University of Connecticut best known in the “microscopy world” as the co-author of the textbook “Transmission Electron Microscopy: A Textbook for Materials Science” as our Microscopy Society of America Invited Speaker. He will have an inspiring talk titled “Challenges for TEM and TEMers: Remember the Past if You Can” in which he links the history of TEM to the current status of the field and its future. Along with Prof. Carter, our invited speakers Prof. Suneel Kodambaka (UCLA), Prof. Dorit Hanein (Sanford-Burnham MRI), Prof. Daniel R. Mumm (UCI) and Dr. Yi-Wei Chang (Caltech) will make our symposium a remarkable event.
In order to register, please RSVP by email at micromark-at-juno.com. Respond no later than 5:00 p.m. Friday, January 31st, 2014 (indicate your special needs by this due).
The cost is $25 for professionals and $10 for students. Membership dues can be paid at the door on February 8th, 2014.
Nonmembers are welcome to attend.
Please pass along to those you feel would be interested.
We look forward to seeing you at the meeting!
http://www.scsmm.org/
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==============================Original Headers============================== 21, 39 -- From nestor.zaluzec-at-gmail.com Wed Jan 15 21:26:17 2014 21, 39 -- Received: from mail-ie0-f174.google.com (mail-ie0-f174.google.com [209.85.223.174]) 21, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0G3QHWo018614 21, 39 -- for {microscopy-at-microscopy.com} ; Wed, 15 Jan 2014 21:26:17 -0600 21, 39 -- Received: by mail-ie0-f174.google.com with SMTP id lx4so3234332iec.19 21, 39 -- for {microscopy-at-microscopy.com} ; Wed, 15 Jan 2014 19:26:17 -0800 (PST) 21, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 21, 39 -- d=gmail.com; s=20120113; 21, 39 -- h=sender:message-id:date:from:reply-to:user-agent:mime-version:to 21, 39 -- :subject:references:in-reply-to:content-type 21, 39 -- :content-transfer-encoding; 21, 39 -- bh=q9pkbdLoFYERF3qnMTaLbZtCthn6ccMgbVVnP2X/ilg=; 21, 39 -- b=iFqmZayr/MG56l5Fj8kYfJKwXOJ6yWJV0IiATzJ79o8x/jg97Y99mCQEwxh72e16gn 21, 39 -- 4/ZN325cDRfxvmnAWbGfYo7QvampOMbUBVpBsfgltDYu5wMVl+HJ/pwamM2m4lJon3il 21, 39 -- sKPk/XUMZgUS69gBpWFziRwEqF3j6q4XWpM118RC8JaxJSDZ4SK73P+ODMoDjOHjFZJg 21, 39 -- LxgrZ3cLc8OcRpYbdvPtNCPcMnmpxiPrfiEb0aE8xPxGRgZL8u8bs/BLuIyz0DPk+WWe 21, 39 -- xS2yTD9kBoikuLVeBQZiwQcF6BvYZrmq8rr0FBpGBy9NQaDIJSVYk1GX9c/jwTaylJGX 21, 39 -- UATQ== 21, 39 -- X-Received: by 10.42.62.196 with SMTP id z4mr5637278ich.49.1389842777498; 21, 39 -- Wed, 15 Jan 2014 19:26:17 -0800 (PST) 21, 39 -- Received: from NestorPwrBk.local (adsl-99-141-123-144.dsl.chcgil.sbcglobal.net. [99.141.123.144]) 21, 39 -- by mx.google.com with ESMTPSA id kt2sm1024702igb.1.2014.01.15.19.26.13 21, 39 -- for {microscopy-at-microscopy.com} 21, 39 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 21, 39 -- Wed, 15 Jan 2014 19:26:16 -0800 (PST) 21, 39 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 21, 39 -- Message-ID: {52D75147.5040201-at-microscopy.com} 21, 39 -- Date: Wed, 15 Jan 2014 21:25:59 -0600 21, 39 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 21, 39 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 39 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 21, 39 -- MIME-Version: 1.0 21, 39 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 39 -- Subject: viaWWW:SCSMM full-day symposium February 8th 2014 21, 39 -- References: {201401160106.s0G163B9017479-at-ns.microscopy.com} 21, 39 -- In-Reply-To: {201401160106.s0G163B9017479-at-ns.microscopy.com} 21, 39 -- X-Forwarded-Message-Id: {201401160106.s0G163B9017479-at-ns.microscopy.com} 21, 39 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 39 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We are keeping our XP machine unplugged from the network, and I made sure to turn off "autorun" for all media (CD/DVD and USB) so infected media can't take over your machine automatically (unless there's a new way to get Windows to automatically execute files, which wouldn't surprise me).
Cheers,
Nick Botto Microprobe Specialist UC Davis Earth and Planetary Sciences Microprobe Lab Website: http://microprobe.geology.ucdavis.edu/index.html Calendar: http://microprobe.geology.ucdavis.edu/calendar/probe.htm ph. 530-752-6582
On 1/15/14, 1:21 PM, John.Mardinly-at-asu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } April 8, 2014 marks the end of support from Microsoft for Windows XP. That means no more security updates to patch newly discovered security holes. This will leave any computers still running Windows XP vulnerable to attacks if they remain connected to the internet or if infected memory media are inserted into the computer. I am sure there are a lot of machines out there running Windows XP. I am curious to hear what approaches microscopy community members are taking to deal with this, as well as what the community of microscopy related hardware vendors are doing to address this situation. Thank you. } } John Mardinly, ASU } } } } ==============================Original Headers============================== } 4, 35 -- From John.Mardinly-at-asu.edu Wed Jan 15 15:12:14 2014 } 4, 35 -- Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) } 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0FLCEU1008827 } 4, 35 -- for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 15:12:14 -0600 } 4, 35 -- X-ASG-Debug-ID: 1389820333-0549c456250a750001-FOsErg } 4, 35 -- Received: from exhubw01.asurite.ad.asu.edu (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnetw3.asu.edu with ESMTP id Lezl3NXq5dB4vpTh (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 14:12:13 -0700 (MST) } 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 } 4, 35 -- X-ASG-Whitelist: Client } 4, 35 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by } 4, 35 -- exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id 14.02.0309.002; } 4, 35 -- Wed, 15 Jan 2014 14:12:12 -0700 } 4, 35 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 4, 35 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} } 4, 35 -- Subject: Windows XP Support Ending April 8, 2014 } 4, 35 -- Thread-Topic: Windows XP Support Ending April 8, 2014 } 4, 35 -- X-ASG-Orig-Subj: Windows XP Support Ending April 8, 2014 } 4, 35 -- Thread-Index: Ac8SNnTksb2ozlmjTzO6+BJCz2aDAQ== } 4, 35 -- Date: Wed, 15 Jan 2014 21:12:11 +0000 } 4, 35 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400B7A2850-at-exmbt05.asurite.ad.asu.edu} } 4, 35 -- Accept-Language: en-US } 4, 35 -- Content-Language: en-US } 4, 35 -- X-MS-Has-Attach: } 4, 35 -- X-MS-TNEF-Correlator: } 4, 35 -- x-originating-ip: [129.219.103.4] } 4, 35 -- Content-Type: text/plain; charset="us-ascii" } 4, 35 -- MIME-Version: 1.0 } 4, 35 -- X-Barracuda-Connect: exhubw01.asurite.ad.asu.edu[129.219.4.199] } 4, 35 -- X-Barracuda-Start-Time: 1389820333 } 4, 35 -- X-Barracuda-Encrypted: AES128-SHA } 4, 35 -- X-Barracuda-URL: http://149.169.2.73:8000/cgi-mod/mark.cgi } 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu } 4, 35 -- X-Barracuda-BRTS-Status: 1 } 4, 35 -- Content-Transfer-Encoding: 8bit } 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0FLCEU1008827 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 23 -- From nwbotto-at-ucdavis.edu Thu Jan 16 12:13:31 2014 5, 23 -- Received: from msa1.ucdavis.edu (msa1.ucdavis.edu [128.120.32.181]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0GIDUKG029552 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 12:13:30 -0600 5, 23 -- Received: from distortedreality.local ([169.237.180.55]) 5, 23 -- (authenticated bits=0) 5, 23 -- by msa1.ucdavis.edu (8.14.4/8.14.5/it-rhel5-mimedefang-smtp-1.6) with ESMTP id s0GIDNGU061882 5, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-CAMELLIA256-SHA bits=256 verify=NO) 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 10:13:24 -0800 5, 23 -- Message-ID: {52D82143.9030301-at-ucdavis.edu} 5, 23 -- Date: Thu, 16 Jan 2014 10:13:23 -0800 5, 23 -- From: Nick Botto {nwbotto-at-ucdavis.edu} 5, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 5, 23 -- MIME-Version: 1.0 5, 23 -- To: microscopy-at-microscopy.com 5, 23 -- Subject: Re: [Microscopy] Windows XP Support Ending April 8, 2014 5, 23 -- References: {201401152121.s0FLLWhR023627-at-ns.microscopy.com} 5, 23 -- In-Reply-To: {201401152121.s0FLLWhR023627-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-Virus-Scanned: clamav-milter 0.97.8 at msa1 5, 23 -- X-Virus-Status: Clean 5, 23 -- X-Scanned-By: MIMEDefang 2.74 ==============================End of - Headers==============================
As an addendum to this, Microsoft announced on January 15th that it would continue to provide updates for security products until July 14, 2015. So no more system updates, but at least they'll continue to update Security Essentials and other MS antivirus signatures for machines running XP.
-- Adam Schuetze, D.Tech, B.Eng Laboratory Engineer, Advanced Microscopy Facility Bob Wright A015, University of Victoria, Canada adamschu-at-uvic.ca Lab: 250.853.3968 http://www.stehm.uvic.ca
-----Original Message----- X-from: nwbotto-at-ucdavis.edu [mailto:nwbotto-at-ucdavis.edu] Sent: Thursday, January 16, 2014 10:28 AM To: Adam Schuetze
We are keeping our XP machine unplugged from the network, and I made sure to turn off "autorun" for all media (CD/DVD and USB) so infected media can't take over your machine automatically (unless there's a new way to get Windows to automatically execute files, which wouldn't surprise me).
Cheers,
Nick Botto Microprobe Specialist UC Davis Earth and Planetary Sciences Microprobe Lab Website: http://microprobe.geology.ucdavis.edu/index.html Calendar: http://microprobe.geology.ucdavis.edu/calendar/probe.htm ph. 530-752-6582
On 1/15/14, 1:21 PM, John.Mardinly-at-asu.edu wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } April 8, 2014 marks the end of support from Microsoft for Windows XP. That means no more security updates to patch newly discovered security holes. This will leave any computers still running Windows XP vulnerable to attacks if they remain connected to the internet or if infected memory media are inserted into the computer. I am sure there are a lot of machines out there running Windows XP. I am curious to hear what approaches microscopy community members are taking to deal with this, as well as what the community of microscopy related hardware vendors are doing to address this situation. Thank you. } } John Mardinly, ASU } } } } ==============================Original } Headers============================== } 4, 35 -- From John.Mardinly-at-asu.edu Wed Jan 15 15:12:14 2014 4, 35 -- } Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) } 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0FLCEU1008827 } 4, 35 -- for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 15:12:14 -0600 } 4, 35 -- X-ASG-Debug-ID: 1389820333-0549c456250a750001-FOsErg } 4, 35 -- Received: from exhubw01.asurite.ad.asu.edu } (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnetw3.asu.edu with } ESMTP id Lezl3NXq5dB4vpTh (version=TLSv1 cipher=AES128-SHA bits=128 } verify=NO) for {Microscopy-at-Microscopy.com} ; Wed, 15 Jan 2014 14:12:13 } -0700 (MST) 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 4, 35 -- } X-ASG-Whitelist: Client 4, 35 -- Received: from } EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 4, 35 -- } exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id } 14.02.0309.002; 4, 35 -- Wed, 15 Jan 2014 14:12:12 -0700 4, 35 -- } From: John Mardinly {John.Mardinly-at-asu.edu} 4, 35 -- To: } "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 4, 35 -- } Subject: Windows XP Support Ending April 8, 2014 4, 35 -- } Thread-Topic: Windows XP Support Ending April 8, 2014 4, 35 -- } X-ASG-Orig-Subj: Windows XP Support Ending April 8, 2014 4, 35 -- } Thread-Index: Ac8SNnTksb2ozlmjTzO6+BJCz2aDAQ== 4, 35 -- Date: Wed, 15 } Jan 2014 21:12:11 +0000 4, 35 -- Message-ID: } {4FFC63ED830B1C41BACD3ABA182BE9400B7A2850-at-exmbt05.asurite.ad.asu.edu} } 4, 35 -- Accept-Language: en-US } 4, 35 -- Content-Language: en-US } 4, 35 -- X-MS-Has-Attach: } 4, 35 -- X-MS-TNEF-Correlator: } 4, 35 -- x-originating-ip: [129.219.103.4] 4, 35 -- Content-Type: } text/plain; charset="us-ascii" } 4, 35 -- MIME-Version: 1.0 } 4, 35 -- X-Barracuda-Connect: } exhubw01.asurite.ad.asu.edu[129.219.4.199] } 4, 35 -- X-Barracuda-Start-Time: 1389820333 4, 35 -- } X-Barracuda-Encrypted: AES128-SHA 4, 35 -- X-Barracuda-URL: } http://149.169.2.73:8000/cgi-mod/mark.cgi } 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 35 -- } X-Barracuda-BRTS-Status: 1 4, 35 -- Content-Transfer-Encoding: 8bit 4, } 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s0FLCEU1008827 ==============================End } of - Headers============================== }
==============================Original Headers============================== 5, 23 -- From nwbotto-at-ucdavis.edu Thu Jan 16 12:13:31 2014 5, 23 -- Received: from msa1.ucdavis.edu (msa1.ucdavis.edu [128.120.32.181]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0GIDUKG029552 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 12:13:30 -0600 5, 23 -- Received: from distortedreality.local ([169.237.180.55]) 5, 23 -- (authenticated bits=0) 5, 23 -- by msa1.ucdavis.edu (8.14.4/8.14.5/it-rhel5-mimedefang-smtp-1.6) with ESMTP id s0GIDNGU061882 5, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-CAMELLIA256-SHA bits=256 verify=NO) 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 10:13:24 -0800 5, 23 -- Message-ID: {52D82143.9030301-at-ucdavis.edu} 5, 23 -- Date: Thu, 16 Jan 2014 10:13:23 -0800 5, 23 -- From: Nick Botto {nwbotto-at-ucdavis.edu} 5, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 5, 23 -- MIME-Version: 1.0 5, 23 -- To: microscopy-at-microscopy.com 5, 23 -- Subject: Re: [Microscopy] Windows XP Support Ending April 8, 2014 5, 23 -- References: {201401152121.s0FLLWhR023627-at-ns.microscopy.com} 5, 23 -- In-Reply-To: {201401152121.s0FLLWhR023627-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-Virus-Scanned: clamav-milter 0.97.8 at msa1 5, 23 -- X-Virus-Status: Clean 5, 23 -- X-Scanned-By: MIMEDefang 2.74 ==============================End of - Headers==============================
==============================Original Headers============================== 14, 31 -- From adamschu-at-uvic.ca Thu Jan 16 13:31:21 2014 14, 31 -- Received: from mole.comp.uvic.ca (mole.comp.uvic.ca [142.104.5.70]) 14, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0GJVKdY020853 14, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Jan 2014 13:31:21 -0600 14, 31 -- Received: from LONGFIN.uvic.ca (longfin.uvic.ca [142.104.240.132]) 14, 31 -- by mole.comp.uvic.ca (8.14.4/8.14.4) with ESMTP id s0GJVJWO018632 14, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Jan 2014 11:31:19 -0800 14, 31 -- Received: from OPAH.uvic.ca ([fe80::ac3a:7aab:abe5:2938]) by LONGFIN.uvic.ca 14, 31 -- ([fe80::200:5efe:142.104.240.132%11]) with mapi id 14.03.0174.001; Thu, 16 14, 31 -- Jan 2014 11:31:19 -0800 14, 31 -- From: Adam Schuetze {adamschu-at-uvic.ca} 14, 31 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 14, 31 -- Subject: RE: [Microscopy] Re: Windows XP Support Ending April 8, 2014 14, 31 -- Thread-Topic: [Microscopy] Re: Windows XP Support Ending April 8, 2014 14, 31 -- Thread-Index: AQHPEui/YjZpFKYJTkyeyiurYTnuz5qHu/qg 14, 31 -- Date: Thu, 16 Jan 2014 19:31:18 +0000 14, 31 -- Message-ID: {3F1048D9CBC6AA4092B13141A4B0F44C1590B571-at-opah.uvic.ca} 14, 31 -- References: {201401161828.s0GISOEJ015766-at-ns.microscopy.com} 14, 31 -- In-Reply-To: {201401161828.s0GISOEJ015766-at-ns.microscopy.com} 14, 31 -- Accept-Language: en-CA, en-US 14, 31 -- Content-Language: en-US 14, 31 -- X-MS-Has-Attach: 14, 31 -- X-MS-TNEF-Correlator: 14, 31 -- x-originating-ip: [142.104.193.193] 14, 31 -- Content-Type: text/plain; charset="us-ascii" 14, 31 -- MIME-Version: 1.0 14, 31 -- X-UVic-Virus-Scanned: OK - Passed virus scan by ClamAV (clamd) on mole 14, 31 -- X-UVic-Scan: mole.comp.uvic.ca filter_version 3.7.6 14, 31 -- X-Scanned-By: MIMEDefang 2.67 on 142.104.5.77 14, 31 -- Content-Transfer-Encoding: 8bit 14, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0GJVKdY020853 ==============================End of - Headers==============================
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Message: Does anyone know what the heat load that the CM10 and CM12 generate?
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 16 17:53:48 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0GNrmsa018377 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 17:53:48 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id 591A86A9D58 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 17:53:48 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id wKvCqzg1f-XA for {microscopy-at-microscopy.com} ; 12, 28 -- Thu, 16 Jan 2014 17:53:43 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id CB2956A9D46 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jan 2014 17:53:43 -0600 (CST) 12, 28 -- Message-ID: {52D87107.3070502-at-microscopy.com} 12, 28 -- Date: Thu, 16 Jan 2014 17:53:43 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:CM10/12 heat production 12, 28 -- References: {201401161941.s0GJfnim006532-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201401161941.s0GJfnim006532-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201401161941.s0GJfnim006532-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I've got the heat load specs for the CM-10 - just have to dig them out. I'll send you a pdf. Not for the CM-12, but they won't be too different.
Phil
} Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10/12 heat production } } Message: Does anyone know what the heat load that the CM10 and CM12 generate? } -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 29 -- From oshel1pe-at-cmich.edu Fri Jan 17 07:06:44 2014 4, 29 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0HD6haQ005955 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Jan 2014 07:06:44 -0600 4, 29 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75] (may be forged)) 4, 29 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s0HCxcJZ004736; 4, 29 -- Fri, 17 Jan 2014 07:59:38 -0500 4, 29 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 4, 29 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 29 -- 14.3.123.3; Fri, 17 Jan 2014 08:06:41 -0500 4, 29 -- Message-ID: {52D92ADC.1040402-at-cmich.edu} 4, 29 -- Date: Fri, 17 Jan 2014 08:06:36 -0500 4, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 29 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 29 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 29 -- MIME-Version: 1.0 4, 29 -- To: {jcsmtf-at-mail.missouri.edu} , micro {Microscopy-at-microscopy.com} 4, 29 -- Subject: Re: [Microscopy] viaWWW:CM10/12 heat production 4, 29 -- References: {201401170008.s0H088tc003794-at-ns.microscopy.com} 4, 29 -- In-Reply-To: {201401170008.s0H088tc003794-at-ns.microscopy.com} 4, 29 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 29 -- Content-Transfer-Encoding: 7bit 4, 29 -- X-Originating-IP: [141.209.160.122] 4, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 29 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 29 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 29 -- X-CanItPRO-Stream: default 4, 29 -- X-Canit-Stats-ID: 05Lf0XCzD - 43de1d4dd168 - 20140117 4, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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Email: sliliensiek-at-ibidiusa.com Name: Sara Liliensiek
Organization: ibidi, LLC
Title-Subject: [Filtered] ibidi, LLC is Now Hiring for a Techincal Sales Representative
Message: Hello Everyone,
ibidi, LLC, a leader in providing solutions for BioMicroscopy, is looking to add a new Technical Sales Representative to the US team. ibidi is an international company which began in Munich Germany and has now expanded in the US. We are looking for a motivated and technical individual to help grow and manage new territories in the East and/or West Coast market. This position is geared towards someone who is dedicated to life sciences and cellular research but is more suited to work in the field rather than the lab.
The successful candidate will be a self-starter with strong communication skills as well as a forward thinker. This is an amazing opportunity to work for an innovative and fast growing company and the correct individual will be provided the tools and support to advance quickly.
If interested, please send your Cover Letter, CV along with salary requirements to Jenni Poole at jpoole-at-ibidi.com.
For a more detailed job description please go to http://ibidi.com/about-ibidi/career/technical-sales-representative/
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 17 09:17:10 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0HFHAPv029038 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jan 2014 09:17:10 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id EFF836AA4B0 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jan 2014 09:17:09 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id enULFILziQBT for {microscopy-at-microscopy.com} ; 19, 28 -- Fri, 17 Jan 2014 09:17:08 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 8DA6A6AA4A3 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jan 2014 09:17:08 -0600 (CST) 19, 28 -- Message-ID: {52D94974.3010404-at-microscopy.com} 19, 28 -- Date: Fri, 17 Jan 2014 09:17:08 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:ibidi, LLC is Now Hiring for a Techincal Sales Representative 19, 28 -- References: {201401171404.s0HE4Yf0025912-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201401171404.s0HE4Yf0025912-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201401171404.s0HE4Yf0025912-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: mpittman-at-hku.hk Name: Michael Pittman
Organization: Department of Earth Sciences, The University of Hong Kong
Title-Subject: [Filtered] Stereoscope with a camera mount for studying dinosaur fossils
Message: Hi there,
My name is Michael Pittman and I am a British Research Assistant Professor investigating the evolution of carnivorous dinosaurs at the University of Hong Kong.
I am looking to purchase my first stereomicroscope for my work and I am a novice towards the science of microscopy.
I have a list of things I am looking for in my setup having asked some colleagues for advice and having looked into the models I used previously. If anyone has or knows of anyone who has equipment for sale that can meet most of the requirements below it would be really great to hear from you.
-Leica, Wild or Olympus brand -~x4 to x100 total magnification range -Camera mount for a DSLR -UV illumination port -Large boom stand for sliding vertically and horizontally over my specimens (these are usually on rock slabs up to 3ft squared) -ring light -A maximum budget of USD$6500-7000 (my understanding is that I would probably only be able to afford a second-hand microscope which is totally fine)
Thanks very much for considering my request.
Best regards, Michael
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Title-Subject: [Filtered] Electron Microscopist Position Available - The Rockefeller University
Message: Title-Subject: [Filtered] Electron Microscopist Position Available
Message: Electron Microscopist, The Rockefeller University, NY, NY
The Rockefeller University, a premier biomedical research institution, seeks a Research Support Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state of the art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a free substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Specialist will participate in all of the Electron Microscopy Resource Center's (EMRC) daily operations, including bench work in preparation for transmission and scanning electron microscopy and maintenance of the center. Will be responsible for all parts of sample preparation, including cutting ultrathin sections, maintenance and operation of EM and other equipment, training users, consulting with scientists on design of experiments, processing/analyzing data and interpretation of results, ordering and receiving supplies, managing chemical waste compliance, administrative support for office duties and center billing, and keeping up with the latest methodology via familiarity with the literature. Ph.D. in biology, cell biology, bioengineering or a related field required. Must have a minimum of 5 years of hands-on experience in electron microscopy and have strong communication skills, and the ability to work collaboratively on a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.
Our culture *inspiring, collaborative atmosphere *strong social and environmental consciousness *intellectually curious academic environment *14-acre campus setting in NYC
We offer a competitive salary, comprehensive benefits, and a beautiful work environment.
To apply to this job, click the following URL, click on 'staff opportunities’ and enter keyword ‘IRC14819’: http://www.rockefeller.edu/hr/career.php
The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Kunihiro Uryu, Ph.D., Director of Electron Microscopy Resource Center (EMRC) The Rockefeller University 1230 York Ave., Box 230 New York, NY 10065
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Email: marzena_sujkowska-at-sggw.pl Name: Marzena
Organization: Warsaw University of Life Sciences, Poland
Title-Subject: [Filtered] Mycorrhiza
Message:
How stain the roots with mycorrhiza for arbuscules before maunting in EPON resin for TEM microscopy?
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 21 07:00:05 2014 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0LD05xS025728 15, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 07:00:05 -0600 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id 6F50E6ACC4C 15, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 07:00:05 -0600 (CST) 15, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id IgYVdtoDsvY8 for {microscopy-at-microscopy.com} ; 15, 28 -- Tue, 21 Jan 2014 07:00:01 -0600 (CST) 15, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 19D806ACC3A 15, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 07:00:01 -0600 (CST) 15, 28 -- Message-ID: {52DE6F50.8010802-at-microscopy.com} 15, 28 -- Date: Tue, 21 Jan 2014 07:00:00 -0600 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:stain the roots with mycorrhiza 15, 28 -- References: {201401210928.s0L9ShlX022320-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201401210928.s0L9ShlX022320-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201401210928.s0L9ShlX022320-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
we find strange bubbles when embedding Bacillus subtilis bacteria in LR white resin. The bubbles prevent the resin from polymerising, does anybody have an idea how and why this could happen?
These bubbles seem to be trapped air in the resin. Put the specimens in the final resin (in the embedding capsules) and left them for an hour. The air bubbles will come to the surface and you can easily break them with a needle. Then put the capsules to the oven for polymerisation.
Panos
Quoting stefan.wernitznig-at-medunigraz.at:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } we find strange bubbles when embedding Bacillus subtilis bacteria in LR } white resin. The bubbles prevent the resin from polymerising, does } anybody have an idea how and why this could happen? } } http://chimerism.medunigraz.at/Pictures/pioloform01.php } http://chimerism.medunigraz.at/Pictures/pioloform02.php } } Many thanks, } Stefan } } } ==============================Original Headers============================== } 5, 30 -- From stefan.wernitznig-at-medunigraz.at Tue Jan 21 09:30:56 2014 } 5, 30 -- Received: from si069.medunigraz.at (si082.medunigraz.at } [193.170.105.82]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s0LFUt90018127 } 5, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 09:30:56 -0600 } 5, 30 -- X-AuditID: 0ac80c45-f79db6d000001194-e2-52de92ae462d } 5, 30 -- Received: from si063.medunigraz.at ( [10.200.18.79]) } 5, 30 -- by si069.medunigraz.at (Symantec Mail Security) with SMTP } id 51.2C.04500.EA29ED25; Tue, 21 Jan 2014 16:30:54 +0100 (CET) } 5, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at } [10.200.112.128]) } 5, 30 -- by si063.medunigraz.at with ESMTP (TLS encrypted); Tue, 21 } Jan 2014 16:30:47 +0100 } 5, 30 -- Message-ID: {52DE92A9.9030803-at-medunigraz.at} } 5, 30 -- Date: Tue, 21 Jan 2014 16:30:49 +0100 } 5, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} } 5, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) } Gecko/20130801 Thunderbird/17.0.8 } 5, 30 -- MIME-Version: 1.0 } 5, 30 -- To: Microscopy-at-microscopy.com } 5, 30 -- Subject: Strange bubbles in LR white resin } 5, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 5, 30 -- Content-Transfer-Encoding: 7bit } 5, 30 -- X-Brightmail-Tracker: } H4sIAAAAAAAAA+NgFtrGIsWRmVeSWpSXmKPExsXCdULIX3fdpHtBBvsalSz+HG5gd2D0+Nj9 } 5, 30 -- } mimAMYrLJiU1J7MstUjfLoErY2uTQ8FTpoq5zyeyNDCuZupi5OSQEDCReNTylgXCFpO4cG89 } 5, 30 -- } WxcjF4eQwHpGiW//97NDOPMYJTZvnsXaxcjBwSugIzGrMRikgUVAVeLltjNsIDabgKXE8e4f } 5, 30 -- } YINEBYIldj9+xgpi8woISpyc+QQsLiIgK7Hg/RF2EFtYQEuiYfNiMJtZwEbi74lFLBC2vMT2 } 5, 30 -- } t3OYJzDyzkLSPgtJ2SwkZQsYmVcxChdnGphZ6uWmppTmZaYXJVbpJZZsYgQHDY/rDsZdTcKH } 5, 30 -- } GAU4GJV4eB913AsSYk0sK67MPcQoycGkJMobMxEoxJeUn1KZkVicEV9UmpNafIhRgoNZSYS3 } 5, 30 -- } bAJQjjclsbIqtSgfJiXNwaIkzrtF4m6QkEB6YklqdmpqQWoRTFaGg0NJgrcOZKhgUWp6akVa } 5, 30 -- } Zk4JQpqJgxNkOA/Q8DkgNbzFBYm5xZnpEPlTjKoc+1Z8+sMoxJKXn5cqJc77BeQCAZCijNI8 } 5, 30 -- } uDmvGMWB3hHmLQYZwQNMDHATXgENZwIafvvSHZDhJYkIKakGxjDeyX5bznE99pK4zPFK7ToP } 5, 30 -- } 75HXXFcf3Hnw6ul1yUfzBT7t43Rj5fokYNR71qhGLlaaN2yH+xbfw1Lfrx3M4zlZ+8T3oZ+P } 5, 30 -- } nt4MS7177Bf1ZgTOef442XLH930Wd16uT71mvHkd8/a+W2yla0P4y32Y1+5rucm7qic++emH } 5, 30 -- y49nsdS7fjth16bEUpyRaKjFXFScCABGPUGSyQIAAA== } ==============================End of - Headers==============================
-- Dr. Berillis Panagiotis Lecturer in Microscopy, image analysis and histology Department of Ichthyology and Aquatic Environment Agricultural school University of Thessaly
==============================Original Headers============================== 8, 21 -- From pveril-at-apae.uth.gr Tue Jan 21 13:53:04 2014 8, 21 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0LJr3KI015109 8, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 13:53:04 -0600 8, 21 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) 8, 21 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0LJreSM013826; 8, 21 -- Tue, 21 Jan 2014 21:53:40 +0200 8, 21 -- Received: from adsl-213.91.140.88.tellas.gr (adsl-213.91.140.88.tellas.gr 8, 21 -- [91.140.88.213]) by webmail.uth.gr (Horde Framework) with HTTP; Tue, 21 Jan 8, 21 -- 2014 21:52:52 +0200 8, 21 -- Date: Tue, 21 Jan 2014 21:52:52 +0200 8, 21 -- Message-ID: {20140121215252.Horde.5K4CvlZsTmoxhFGI1v_E0w1-at-webmail.uth.gr} 8, 21 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} 8, 21 -- To: stefan.wernitznig-at-medunigraz.at, Microscopy-at-microscopy.com 8, 21 -- Subject: Re: [Microscopy] Strange bubbles in LR white resin 8, 21 -- References: {201401211539.s0LFds8O029163-at-ns.microscopy.com} 8, 21 -- In-Reply-To: {201401211539.s0LFds8O029163-at-ns.microscopy.com} 8, 21 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) 8, 21 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Disposition: inline ==============================End of - Headers==============================
Those don't look like air bubbles to me. They look to being something extracted from the bacteria. Maybe not fully dehydrated? Some non-miscible solvent still around? Air bubbles shouldn't prevent LRW from polymerizing. Does a "blank" block with no cells polymerize?
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: pveril-at-apae.uth.gr [mailto:pveril-at-apae.uth.gr] Sent: Tuesday, January 21, 2014 1:54 PM To: Phillips, Thomas E.
Dear Stefan
These bubbles seem to be trapped air in the resin. Put the specimens in the final resin (in the embedding capsules) and left them for an hour. The air bubbles will come to the surface and you can easily break them with a needle. Then put the capsules to the oven for polymerisation.
Panos
Quoting stefan.wernitznig-at-medunigraz.at:
} ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Dear Listers, } } we find strange bubbles when embedding Bacillus subtilis bacteria in } LR white resin. The bubbles prevent the resin from polymerising, does } anybody have an idea how and why this could happen? } } http://chimerism.medunigraz.at/Pictures/pioloform01.php } http://chimerism.medunigraz.at/Pictures/pioloform02.php } } Many thanks, } Stefan } } } ==============================Original } Headers============================== } 5, 30 -- From stefan.wernitznig-at-medunigraz.at Tue Jan 21 09:30:56 2014 } 5, 30 -- Received: from si069.medunigraz.at (si082.medunigraz.at } [193.170.105.82]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s0LFUt90018127 } 5, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 09:30:56 -0600 } 5, 30 -- X-AuditID: 0ac80c45-f79db6d000001194-e2-52de92ae462d } 5, 30 -- Received: from si063.medunigraz.at ( [10.200.18.79]) } 5, 30 -- by si069.medunigraz.at (Symantec Mail Security) with SMTP } id 51.2C.04500.EA29ED25; Tue, 21 Jan 2014 16:30:54 +0100 (CET) 5, 30 } -- Received: from [10.200.112.128] (dc112128.medunigraz.at } [10.200.112.128]) } 5, 30 -- by si063.medunigraz.at with ESMTP (TLS encrypted); Tue, 21 } Jan 2014 16:30:47 +0100 } 5, 30 -- Message-ID: {52DE92A9.9030803-at-medunigraz.at} 5, 30 -- Date: } Tue, 21 Jan 2014 16:30:49 +0100 5, 30 -- From: Stefan Wernitznig } {stefan.wernitznig-at-medunigraz.at} 5, 30 -- User-Agent: Mozilla/5.0 } (Windows NT 5.1; rv:17.0) } Gecko/20130801 Thunderbird/17.0.8 } 5, 30 -- MIME-Version: 1.0 } 5, 30 -- To: Microscopy-at-microscopy.com 5, 30 -- Subject: Strange } bubbles in LR white resin 5, 30 -- Content-Type: text/plain; } charset=ISO-8859-15; format=flowed 5, 30 -- Content-Transfer-Encoding: } 7bit 5, 30 -- X-Brightmail-Tracker: } H4sIAAAAAAAAA+NgFtrGIsWRmVeSWpSXmKPExsXCdULIX3fdpHtBBvsalSz+HG5gd2D0+N } H4sIAAAAAAAAA+NgFtrGIsWRmVeSWpSXmKPExsXCdULIX3fdpHtBBvsalSz+HG5gd2D0+j } H4sIAAAAAAAAA+NgFtrGIsWRmVeSWpSXmKPExsXCdULIX3fdpHtBBvsalSz+HG5gd2D0+9 } 5, 30 -- } } mimAMYrLJiU1J7MstUjfLoErY2uTQ8FTpoq5zyeyNDCuZupi5OSQEDCReNTylgXCFpO4cG } 89 } 5, 30 -- } } WxcjF4eQwHpGiW//97NDOPMYJTZvnsXaxcjBwSugIzGrMRikgUVAVeLltjNsIDabgKXE8e } 4f } 5, 30 -- } } YINEBYIldj9+xgpi8woISpyc+QQsLiIgK7Hg/RF2EFtYQEuiYfNiMJtZwEbi74lFLBC2vM } T2 } 5, 30 -- } } t3OYJzDyzkLSPgtJ2SwkZQsYmVcxChdnGphZ6uWmppTmZaYXJVbpJZZsYgQHDY/rDsZdTc } KH } 5, 30 -- } } GAU4GJV4eB913AsSYk0sK67MPcQoycGkJMobMxEoxJeUn1KZkVicEV9UmpNafIhRgoNZSY } S3 } 5, 30 -- } } bAJQjjclsbIqtSgfJiXNwaIkzrtF4m6QkEB6YklqdmpqQWoRTFaGg0NJgrcOZKhgUWp6ak } Va } 5, 30 -- } } Zk4JQpqJgxNkOA/Q8DkgNbzFBYm5xZnpEPlTjKoc+1Z8+sMoxJKXn5cqJc77BeQCAZCijN } I8 } 5, 30 -- } } uDmvGMWB3hHmLQYZwQNMDHATXgENZwIafvvSHZDhJYkIKakGxjDeyX5bznE99pK4zPFK7T } oP } 5, 30 -- } } 75HXXFcf3Hnw6ul1yUfzBT7t43Rj5fokYNR71qhGLlaaN2yH+xbfw1Lfrx3M4zlZ+8T3oZ } +P } 5, 30 -- } nt4MS7177Bf1ZgTOef442XLH930Wd16uT71mvHkd8/a+W2yla0P4y32Y1+5rucm7qic++emH } 5, 30 -- y49nsdS7fjth16bEUpyRaKjFXFScCABGPUGSyQIAAA== } ==============================End of - } Headers==============================
-- Dr. Berillis Panagiotis Lecturer in Microscopy, image analysis and histology Department of Ichthyology and Aquatic Environment Agricultural school University of Thessaly
==============================Original Headers============================== 8, 21 -- From pveril-at-apae.uth.gr Tue Jan 21 13:53:04 2014 8, 21 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0LJr3KI015109 8, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 13:53:04 -0600 8, 21 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) 8, 21 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0LJreSM013826; 8, 21 -- Tue, 21 Jan 2014 21:53:40 +0200 8, 21 -- Received: from adsl-213.91.140.88.tellas.gr (adsl-213.91.140.88.tellas.gr 8, 21 -- [91.140.88.213]) by webmail.uth.gr (Horde Framework) with HTTP; Tue, 21 Jan 8, 21 -- 2014 21:52:52 +0200 8, 21 -- Date: Tue, 21 Jan 2014 21:52:52 +0200 8, 21 -- Message-ID: {20140121215252.Horde.5K4CvlZsTmoxhFGI1v_E0w1-at-webmail.uth.gr} 8, 21 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} 8, 21 -- To: stefan.wernitznig-at-medunigraz.at, Microscopy-at-microscopy.com 8, 21 -- Subject: Re: [Microscopy] Strange bubbles in LR white resin 8, 21 -- References: {201401211539.s0LFds8O029163-at-ns.microscopy.com} 8, 21 -- In-Reply-To: {201401211539.s0LFds8O029163-at-ns.microscopy.com} 8, 21 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) 8, 21 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 18, 30 -- From PhillipsT-at-missouri.edu Tue Jan 21 14:35:21 2014 18, 30 -- Received: from um-tip1-missouri-out.um.umsystem.edu (um-tip1-missouri-out.um.umsystem.edu [198.209.49.135]) 18, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0LKZLsQ004021 18, 30 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2014 14:35:21 -0600 18, 30 -- X-IronPort-Anti-Spam-Filtered: true 18, 30 -- X-IronPort-Anti-Spam-Result: AhgFAMXZ3lLPoJ7E/2dsb2JhbAA/FgEDgws4VqlskgaBFhZ0giUBAQEEOhEBAjcCAgIBCBEDAQEBCwISCQcWGwEUAwEFCAIECgkIFQSHZA02qwwBkkCEfxcEjHANA4EqAQERDQYbEgUGC4IedYEUBJlUiySFQoMtgXE5 18, 30 -- X-IPAS-Result: AhgFAMXZ3lLPoJ7E/2dsb2JhbAA/FgEDgws4VqlskgaBFhZ0giUBAQEEOhEBAjcCAgIBCBEDAQEBCwISCQcWGwEUAwEFCAIECgkIFQSHZA02qwwBkkCEfxcEjHANA4EqAQERDQYbEgUGC4IedYEUBJlUiySFQoMtgXE5 18, 30 -- Received: from um-tcas1.um.umsystem.edu ([207.160.158.196]) 18, 30 -- by um-tip1-exch-relay.um.umsystem.edu with ESMTP; 21 Jan 2014 14:35:19 -0600 18, 30 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.204]) by 18, 30 -- UM-TCAS1.um.umsystem.edu ([207.160.158.196]) with mapi id 14.03.0169.001; 18, 30 -- Tue, 21 Jan 2014 14:35:19 -0600 18, 30 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 18, 30 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 18, 30 -- Subject: RE: [Microscopy] Re: Strange bubbles in LR white resin 18, 30 -- Thread-Topic: [Microscopy] Re: Strange bubbles in LR white resin 18, 30 -- Thread-Index: AQHPFuKL/s5jc3XgnkO+lpcDg5FRmpqPojAA 18, 30 -- Date: Tue, 21 Jan 2014 20:35:18 +0000 18, 30 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8E7178D-at-UM-MBX-T02.um.umsystem.edu} 18, 30 -- References: {201401211954.s0LJs5Ds016021-at-ns.microscopy.com} 18, 30 -- In-Reply-To: {201401211954.s0LJs5Ds016021-at-ns.microscopy.com} 18, 30 -- Accept-Language: en-US 18, 30 -- Content-Language: en-US 18, 30 -- X-MS-Has-Attach: 18, 30 -- X-MS-TNEF-Correlator: 18, 30 -- x-originating-ip: [128.206.81.115] 18, 30 -- Content-Type: text/plain; charset="us-ascii" 18, 30 -- MIME-Version: 1.0 18, 30 -- Content-Transfer-Encoding: 8bit 18, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0LKZLsQ004021 ==============================End of - Headers==============================
By chance did you happen to post fix the bacteria in osmium tetroxide? LR White does not polymerize well when osmium is used in the processing protocol.
Mary Ard Electron Microscopy Lab Coordinator Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, GA 30602 706-542-5537
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Email: paulwebsterphd-at-gmail.com Name: Paul Webster
Organization: University of Souther California
Title-Subject: [Filtered] LR White and air bubbles
Message: Dear Stephan,
I agree with Tom, that it looks as if something has been released from the bacteria. It could be lipid being extracted by the resin, which is a pretty good solvent.
I am not sure that this is the reason why the resin has not polymerized. From your images, it does look as if you have used osmium tetroxide to contrast the cells. It might also be possible that you also used acetone to dehydrate the cells.
Residual amounts of acetone may have interfered with resin polymerization in the way you describe. A more detailed description of your processing and embedding protocol may help diagnose the problem.
Paul Webster, University of Southern California Los Angeles, CA
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What is the average size of the "particles"? You do not have a size bar in your image to get a feel for their size, which is important in any viral identification. The "particles" do not appear to be associated with the sample on the grid but laying on top of the sample. In my opinion, they look more like crystalline structures rather than viral particles. Depending on their actual size, could they possibly be phosphotungstic acid crystals?
Kind regards, Mary
Mary Ard Electron Microscopy Lab Coordinator Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, GA 30602 706-542-5537
==============================Original Headers============================== 6, 29 -- From maryard-at-uga.edu Thu Jan 23 08:06:42 2014 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NE6gO0014516 6, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 08:06:42 -0600 6, 29 -- Received: from DM2PR02MB480.namprd02.prod.outlook.com (10.141.84.156) by 6, 29 -- DM2PR02MB477.namprd02.prod.outlook.com (10.141.84.143) with Microsoft SMTP 6, 29 -- Server (TLS) id 15.0.859.15; Thu, 23 Jan 2014 14:06:37 +0000 6, 29 -- Received: from DM2PR02MB480.namprd02.prod.outlook.com ([10.141.84.156]) by 6, 29 -- DM2PR02MB480.namprd02.prod.outlook.com ([10.141.84.156]) with mapi id 6, 29 -- 15.00.0859.013; Thu, 23 Jan 2014 14:06:37 +0000 6, 29 -- From: Mary Brown Ard {maryard-at-uga.edu} 6, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 29 -- Subject: [Microscopy] Virus 6, 29 -- Thread-Topic: [Microscopy] Virus 6, 29 -- Thread-Index: Ac8YQxrT3GMwtR30RFK9YaSHozxtFQ== 6, 29 -- Date: Thu, 23 Jan 2014 14:06:36 +0000 6, 29 -- Message-ID: {473dbc6ac41041b38ff74dd69ab35549-at-DM2PR02MB480.namprd02.prod.outlook.com} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- x-originating-ip: [128.192.89.8] 6, 29 -- x-forefront-prvs: 0100732B76 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(71364002)(189002)(199002)(4396001)(74502001)(47446002)(79102001)(83322001)(2656002)(81686001)(81816001)(65816001)(80022001)(75432001)(59766001)(50986001)(85306002)(47976001)(66066001)(81542001)(87266001)(77982001)(33646001)(47736001)(49866001)(87936001)(63696002)(31966008)(92566001)(74662001)(81342001)(80976001)(74876001)(76576001)(76176001)(76786001)(76796001)(69226001)(85852003)(83072002)(74316001)(93516002)(86362001)(90146001)(93136001)(56816005)(74366001)(51856001)(46102001)(74706001)(54316002)(56776001)(53806001)(76482001)(54356001)(94316002)(24736002);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR02MB477;H:DM2PR02MB480.namprd02.prod.outlook.com;CLIP:128.192.89.8;FPR:;InfoNoRecordsMX:1;A:1;LANG:en; 6, 29 -- Content-Type: text/plain; charset="us-ascii" 6, 29 -- MIME-Version: 1.0 6, 29 -- X-OriginatorOrg: uga.edu 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NE6gO0014516 ==============================End of - Headers==============================
Giving a picture without scale to a miscroscopist is like talking without words. We cannot make anything out if it! I doubt these objects are embedded in the resin. One can see a hole in the section and one object seems to partly cover it. Also they don't look like they were sectioned. Looks like artifacts for me.
Regards, Stephane
________________________________ X-from: "pveril-at-apae.uth.gr" {pveril-at-apae.uth.gr} To: nizets2-at-yahoo.com Sent: Thursday, January 23, 2014 11:29 AM
Dear all
During an electron microscope examination of a Sparus aurata liver collagen (PTA and uranyl acetate were used as stains) I came across to these objects. They are probably virus particles. Can any one identify them?
http://upload.users.uth.gr/files/virus.jpg
Many thanks Panos -- Dr. Berillis Panagiotis Lecturer in Microscopy, image analysis and histology Department of Ichthyology and Aquatic Environment Agricultural school University of Thessaly
==============================Original Headers============================== 6, 18 -- From pveril-at-apae.uth.gr Thu Jan 23 04:23:12 2014 6, 18 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NANBUa024847 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 04:23:12 -0600 6, 18 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) 6, 18 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0NANwCR031164 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 12:23:58 +0200 6, 18 -- Received: from 10.64.60.150 ([10.64.60.150]) by webmail.uth.gr (Horde 6, 18 -- Framework) with HTTP; Thu, 23 Jan 2014 12:23:00 +0200 6, 18 -- Date: Thu, 23 Jan 2014 12:23:00 +0200 6, 18 -- Message-ID: {20140123122300.Horde.--gLtdj-JhwWKO_juLlJ4w3-at-webmail.uth.gr} 6, 18 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: Virus 6, 18 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) 6, 18 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Disposition: inline ==============================End of - Headers==============================
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I agree, it does not looks like belonging to section. Some contamination after sectioning/staining. I have stained a lot with PTA, have never seen something like this.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, January 23, 2014 8:23 AM To: Dusevich, Vladimir
Hi Panos!
Giving a picture without scale to a miscroscopist is like talking without words. We cannot make anything out if it! I doubt these objects are embedded in the resin. One can see a hole in the section and one object seems to partly cover it. Also they don't look like they were sectioned. Looks like artifacts for me.
Regards, Stephane
________________________________ X-from: "pveril-at-apae.uth.gr" {pveril-at-apae.uth.gr} To: nizets2-at-yahoo.com Sent: Thursday, January 23, 2014 11:29 AM
Dear all
During an electron microscope examination of a Sparus aurata liver collagen (PTA and uranyl acetate were used as stains) I came across to these objects. They are probably virus particles. Can any one identify them?
http://upload.users.uth.gr/files/virus.jpg
Many thanks Panos -- Dr. Berillis Panagiotis Lecturer in Microscopy, image analysis and histology Department of Ichthyology and Aquatic Environment Agricultural school University of Thessaly
==============================Original Headers============================== 6, 18 -- From pveril-at-apae.uth.gr Thu Jan 23 04:23:12 2014 6, 18 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NANBUa024847 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 04:23:12 -0600 6, 18 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) 6, 18 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0NANwCR031164 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 12:23:58 +0200 6, 18 -- Received: from 10.64.60.150 ([10.64.60.150]) by webmail.uth.gr (Horde 6, 18 -- Framework) with HTTP; Thu, 23 Jan 2014 12:23:00 +0200 6, 18 -- Date: Thu, 23 Jan 2014 12:23:00 +0200 6, 18 -- Message-ID: {20140123122300.Horde.--gLtdj-JhwWKO_juLlJ4w3-at-webmail.uth.gr} 6, 18 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: Virus 6, 18 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) 6, 18 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Disposition: inline ==============================End of - Headers==============================
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==============================Original Headers============================== 25, 30 -- From DusevichV-at-umkc.edu Thu Jan 23 09:05:08 2014 25, 30 -- Received: from um-nip4-umkc-out.um.umsystem.edu (um-nip4-umkc-out.um.umsystem.edu [198.209.49.180]) 25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NF58WE020492 25, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 09:05:08 -0600 25, 30 -- X-IronPort-Anti-Spam-Filtered: true 25, 30 -- X-IronPort-Anti-Spam-Result: Ag0FADQv4VLPoJ7U/2dsb2JhbABRBgEDgww4Vqh1kn6BEBZ0giUBAQEETjcEAgEIEQMBAQELAhsHMQETAQkIAgQKCQgVBIdQAxENv30DYYR/F4xsCYEvCwEBEQ0GGxIFBguDE4EUBIkQjS2DGIYhhQqFO4MtgXE5 25, 30 -- X-IPAS-Result: Ag0FADQv4VLPoJ7U/2dsb2JhbABRBgEDgww4Vqh1kn6BEBZ0giUBAQEETjcEAgEIEQMBAQELAhsHMQETAQkIAgQKCQgVBIdQAxENv30DYYR/F4xsCYEvCwEBEQ0GGxIFBguDE4EUBIkQjS2DGIYhhQqFO4MtgXE5 25, 30 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 25, 30 -- by um-nip4-exch-relay.um.umsystem.edu with ESMTP; 23 Jan 2014 09:05:07 -0600 25, 30 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.195]) by 25, 30 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0169.001; 25, 30 -- Thu, 23 Jan 2014 09:05:06 -0600 25, 30 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 25, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 25, 30 -- Subject: RE: [Microscopy] Fw: Virus 25, 30 -- Thread-Topic: [Microscopy] Fw: Virus 25, 30 -- Thread-Index: AQHPGEaTIcrCG0fAIkeoPKD7Zb7QHZqSZ9IA 25, 30 -- Date: Thu, 23 Jan 2014 15:05:06 +0000 25, 30 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DBA1AD6-at-UM-MBX-T01.um.umsystem.edu} 25, 30 -- References: {201401231422.s0NEMeo7001595-at-ns.microscopy.com} 25, 30 -- In-Reply-To: {201401231422.s0NEMeo7001595-at-ns.microscopy.com} 25, 30 -- Accept-Language: en-US 25, 30 -- Content-Language: en-US 25, 30 -- X-MS-Has-Attach: 25, 30 -- X-MS-TNEF-Correlator: 25, 30 -- x-originating-ip: [134.193.156.65] 25, 30 -- Content-Type: text/plain; charset="iso-8859-1" 25, 30 -- MIME-Version: 1.0 25, 30 -- Content-Transfer-Encoding: 8bit 25, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NF58WE020492 ==============================End of - Headers==============================
A day without fusion is like a day without sunshine.
On 23/01/2014 11:05 AM, DusevichV-at-umkc.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I agree, it does not looks like belonging to section. Some contamination after sectioning/staining. I have stained a lot with PTA, have never seen something like this. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Director } (816) 235-2072 } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } -----Original Message----- } X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: Thursday, January 23, 2014 8:23 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Fw: Virus } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi Panos! } } Giving a picture without scale to a miscroscopist is like talking without words. We cannot make anything out if it! } I doubt these objects are embedded in the resin. One can see a hole in the section and one object seems to partly cover it. Also they don't look like they were sectioned. } Looks like artifacts for me. } } Regards, } Stephane } } } ________________________________ } X-from: "pveril-at-apae.uth.gr" {pveril-at-apae.uth.gr} } To: nizets2-at-yahoo.com } Sent: Thursday, January 23, 2014 11:29 AM } Subject: [Microscopy] Virus } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear all } } During an electron microscope examination of a Sparus aurata liver } collagen (PTA and uranyl acetate were used as stains) I came across to } these objects. They are probably virus particles. Can any one identify } them? } } http://upload.users.uth.gr/files/virus.jpg } } Many thanks } Panos
==============================Original Headers============================== 14, 21 -- From jehrman-at-mta.ca Thu Jan 23 09:21:14 2014 14, 21 -- Received: from smtpy.mta.ca (smtpy.mta.ca [138.73.1.143]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NFLEHO007014 14, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 09:21:14 -0600 14, 21 -- Received: from host-22-169.mta.ca ([138.73.22.169]:52005) 14, 21 -- by smtpy.mta.ca with esmtpsa (TLSv1:DHE-RSA-CAMELLIA256-SHA:256) 14, 21 -- (Exim 4.82) 14, 21 -- (envelope-from {jehrman-at-mta.ca} ) 14, 21 -- id 1W6M5g-0004Wn-CR 14, 21 -- for Microscopy-at-microscopy.com; Thu, 23 Jan 2014 11:21:12 -0400 14, 21 -- Message-ID: {52E13368.4060708-at-mta.ca} 14, 21 -- Date: Thu, 23 Jan 2014 11:21:12 -0400 14, 21 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 14, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 14, 21 -- MIME-Version: 1.0 14, 21 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 14, 21 -- Subject: Re: [Microscopy] RE: Fw: Virus 14, 21 -- References: {201401231505.s0NF5T6w021030-at-ns.microscopy.com} 14, 21 -- In-Reply-To: {201401231505.s0NF5T6w021030-at-ns.microscopy.com} 14, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Ya sou Panos Yes, scales are different, yet it is remarkable how much your particles resemble to pollen I have a picture from a wild flower pollen that looks very close to these particles http://www.eikonika.net/v2/downloads/KITSTI03_resize.jpg. Unlike in the North America, spring came earlier in Greece this year... Best wishes yorgos
A day without fusion is like a day without sunshine.
I agree, it does not looks like belonging to section. Some contamination after sectioning/staining. I have stained a lot with PTA, have never seen something like this.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, January 23, 2014 8:23 AM To: Dusevich, Vladimir
Hello Everyone, Years ago, maybe 20, I saw SEM images of material observed in diesel exhaust. It's not carbon black, but we never did figure out what it was. It look very much like these images. Any chance it could be environmental contamination?
I seriously doubt its pollen. Most pollen to too, much too big to see in it's entirety in a TEM.
I look forward to finding out what it is!
Frank
-----Original Message----- X-from: maryard-at-uga.edu [mailto:maryard-at-uga.edu] Sent: Thursday, January 23, 2014 9:18 AM To: Frank Karl
Panos,
What is the average size of the "particles"? You do not have a size bar in your image to get a feel for their size, which is important in any viral identification. The "particles" do not appear to be associated with the sample on the grid but laying on top of the sample. In my opinion, they look more like crystalline structures rather than viral particles. Depending on their actual size, could they possibly be phosphotungstic acid crystals?
Kind regards, Mary
Mary Ard Electron Microscopy Lab Coordinator Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, GA 30602 706-542-5537
==============================Original Headers============================== 6, 29 -- From maryard-at-uga.edu Thu Jan 23 08:06:42 2014 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NE6gO0014516 6, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 08:06:42 -0600 6, 29 -- Received: from DM2PR02MB480.namprd02.prod.outlook.com (10.141.84.156) by 6, 29 -- DM2PR02MB477.namprd02.prod.outlook.com (10.141.84.143) with Microsoft SMTP 6, 29 -- Server (TLS) id 15.0.859.15; Thu, 23 Jan 2014 14:06:37 +0000 6, 29 -- Received: from DM2PR02MB480.namprd02.prod.outlook.com ([10.141.84.156]) by 6, 29 -- DM2PR02MB480.namprd02.prod.outlook.com ([10.141.84.156]) with mapi id 6, 29 -- 15.00.0859.013; Thu, 23 Jan 2014 14:06:37 +0000 6, 29 -- From: Mary Brown Ard {maryard-at-uga.edu} 6, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 29 -- Subject: [Microscopy] Virus 6, 29 -- Thread-Topic: [Microscopy] Virus 6, 29 -- Thread-Index: Ac8YQxrT3GMwtR30RFK9YaSHozxtFQ== 6, 29 -- Date: Thu, 23 Jan 2014 14:06:36 +0000 6, 29 -- Message-ID: {473dbc6ac41041b38ff74dd69ab35549-at-DM2PR02MB480.namprd02.prod.outlook.com} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- x-originating-ip: [128.192.89.8] 6, 29 -- x-forefront-prvs: 0100732B76 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(71364002)(189002)(199002)(4396001)(74502001)(47446002)(79102001)(83322001)(2656002)(81686001)(81816001)(65816001)(80022001)(75432001)(59766001)(50986001)(85306002)(47976001)(66066001)(81542001)(87266001)(77982001)(33646001)(47736001)(49866001)(87936001)(63696002)(31966008)(92566001)(74662001)(81342001)(80976001)(74876001)(76576001)(76176001)(76786001)(76796001)(69226001)(85852003)(83072002)(74316001)(93516002)(86362001)(90146001)(93136001)(56816005)(74366001)(51856001)(46102001)(74706001)(54316002)(56776001)(53806001)(76482001)(54356001)(94316002)(24736002);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR02MB477;H:DM2PR02MB480.namprd02.prod.outlook.com;CLIP:128.192.89.8;FPR:;InfoNoRecordsMX:1;A:1;LANG:en; 6, 29 -- Content-Type: text/plain; charset="us-ascii" 6, 29 -- MIME-Version: 1.0 6, 29 -- X-OriginatorOrg: uga.edu 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NE6gO0014516 ==============================End of - Headers==============================
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==============================Original Headers============================== 19, 26 -- From frank_karl-at-ardl.com Thu Jan 23 11:34:30 2014 19, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 19, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NHYQtL014454 19, 26 -- for {microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 11:34:29 -0600 19, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 19, 26 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Thu, 23 19, 26 -- Jan 2014 12:34:25 -0500 19, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 19, 26 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 19, 26 -- {microscopy-at-microscopy.com} 19, 26 -- Date: Thu, 23 Jan 2014 12:34:25 -0500 19, 26 -- Subject: RE: [Microscopy] Virus 19, 26 -- Thread-Topic: [Microscopy] Virus 19, 26 -- Thread-Index: Ac8YRf7pmueARSLYRGOBttU8X/QlqwAGlBCw 19, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BC8C6CF65-at-exchange2k7.ad.ardl.com} 19, 26 -- References: {201401231417.s0NEHphn028174-at-ns.microscopy.com} 19, 26 -- In-Reply-To: {201401231417.s0NEHphn028174-at-ns.microscopy.com} 19, 26 -- Accept-Language: en-US 19, 26 -- Content-Language: en-US 19, 26 -- X-MS-Has-Attach: 19, 26 -- X-MS-TNEF-Correlator: 19, 26 -- acceptlanguage: en-US 19, 26 -- Content-Type: text/plain; charset="us-ascii" 19, 26 -- MIME-Version: 1.0 19, 26 -- Content-Transfer-Encoding: 8bit 19, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NHYQtL014454 ==============================End of - Headers==============================
A day without fusion is like a day without sunshine.
On 23/01/2014 11:05 AM, DusevichV-at-umkc.edu wrote: } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I agree, it does not looks like belonging to section. Some contamination after sectioning/staining. I have stained a lot with PTA, have never seen something like this. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Director } (816) 235-2072 } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } -----Original Message----- } X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: Thursday, January 23, 2014 8:23 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Fw: Virus } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } Hi Panos! } } Giving a picture without scale to a miscroscopist is like talking without words. We cannot make anything out if it! } I doubt these objects are embedded in the resin. One can see a hole in the section and one object seems to partly cover it. Also they don't look like they were sectioned. } Looks like artifacts for me. } } Regards, } Stephane } } } ________________________________ } X-from: "pveril-at-apae.uth.gr" {pveril-at-apae.uth.gr} } To: nizets2-at-yahoo.com } Sent: Thursday, January 23, 2014 11:29 AM } Subject: [Microscopy] Virus } } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } Dear all } } During an electron microscope examination of a Sparus aurata liver } collagen (PTA and uranyl acetate were used as stains) I came across to } these objects. They are probably virus particles. Can any one identify } them? } } http://upload.users.uth.gr/files/virus.jpg } } Many thanks } Panos
==============================Original Headers============================== 14, 21 -- From jehrman-at-mta.ca Thu Jan 23 09:21:14 2014 14, 21 -- Received: from smtpy.mta.ca (smtpy.mta.ca [138.73.1.143]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NFLEHO007014 14, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 09:21:14 -0600 14, 21 -- Received: from host-22-169.mta.ca ([138.73.22.169]:52005) 14, 21 -- by smtpy.mta.ca with esmtpsa (TLSv1:DHE-RSA-CAMELLIA256-SHA:256) 14, 21 -- (Exim 4.82) 14, 21 -- (envelope-from {jehrman-at-mta.ca} ) 14, 21 -- id 1W6M5g-0004Wn-CR 14, 21 -- for Microscopy-at-microscopy.com; Thu, 23 Jan 2014 11:21:12 -0400 14, 21 -- Message-ID: {52E13368.4060708-at-mta.ca} 14, 21 -- Date: Thu, 23 Jan 2014 11:21:12 -0400 14, 21 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 14, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 14, 21 -- MIME-Version: 1.0 14, 21 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 14, 21 -- Subject: Re: [Microscopy] RE: Fw: Virus 14, 21 -- References: {201401231505.s0NF5T6w021030-at-ns.microscopy.com} 14, 21 -- In-Reply-To: {201401231505.s0NF5T6w021030-at-ns.microscopy.com} 14, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 24, 30 -- From DusevichV-at-umkc.edu Thu Jan 23 11:58:05 2014 24, 30 -- Received: from um-nip3-umkc-out.um.umsystem.edu (um-nip3-umkc-out.um.umsystem.edu [198.209.49.166]) 24, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NHw4eZ001045 24, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 11:58:04 -0600 24, 30 -- X-IronPort-Anti-Spam-Filtered: true 24, 30 -- X-IronPort-Anti-Spam-Result: AhUFAIRX4VLPoJ7G/2dsb2JhbABRBwODDDhQBqh9kn6BExZ0giUBAQEEOhQYHwICAgEIEQMBAQEBCgISCQcbFgETAQMBBQgCBAoJCBUEh1ADEQgFwHWEfxcEjGgZgRkGCwEfBhsSBQYLgxOBFASWPYMYiysDhTiDLYFxOQ 24, 30 -- X-IPAS-Result: AhUFAIRX4VLPoJ7G/2dsb2JhbABRBwODDDhQBqh9kn6BExZ0giUBAQEEOhQYHwICAgEIEQMBAQEBCgISCQcbFgETAQMBBQgCBAoJCBUEh1ADEQgFwHWEfxcEjGgZgRkGCwEfBhsSBQYLgxOBFASWPYMYiysDhTiDLYFxOQ 24, 30 -- Received: from um-tcas2.um.umsystem.edu ([207.160.158.198]) 24, 30 -- by um-nip3-exch-relay.um.umsystem.edu with ESMTP; 23 Jan 2014 11:58:03 -0600 24, 30 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.195]) by 24, 30 -- UM-TCAS2.um.umsystem.edu ([207.160.158.198]) with mapi id 14.03.0169.001; 24, 30 -- Thu, 23 Jan 2014 11:58:03 -0600 24, 30 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 24, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 24, 30 -- Subject: RE: [Microscopy] Fw: Virus 24, 30 -- Thread-Topic: [Microscopy] Fw: Virus 24, 30 -- Thread-Index: AQHPGE7PtrREBGJTgUK+m2nXye/P95qSmFQA 24, 30 -- Date: Thu, 23 Jan 2014 17:58:03 +0000 24, 30 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DBA1B68-at-UM-MBX-T01.um.umsystem.edu} 24, 30 -- References: {201401231521.s0NFLa4x007680-at-ns.microscopy.com} 24, 30 -- In-Reply-To: {201401231521.s0NFLa4x007680-at-ns.microscopy.com} 24, 30 -- Accept-Language: en-US 24, 30 -- Content-Language: en-US 24, 30 -- X-MS-Has-Attach: 24, 30 -- X-MS-TNEF-Correlator: 24, 30 -- x-originating-ip: [134.193.156.65] 24, 30 -- Content-Type: text/plain; charset="us-ascii" 24, 30 -- MIME-Version: 1.0 24, 30 -- Content-Transfer-Encoding: 8bit 24, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0NHw4eZ001045 ==============================End of - Headers==============================
I take it that these are sitting on a film and are negatively stained? With BOTH UrAc and PTA? They look vaguely like something tentatively identified as scales from some sort of phytoplankton that we often see in marine preps. They do not look like any of the many marine viruses we've seen here, but I will show them to someone who works on marine viruses and see what they say. However, right now I'm going with the idea that they are stain crystals!
Aloha, Tina
} During an electron microscope examination of a Sparus aurata liver } collagen (PTA and uranyl acetate were used as stains) I came across to } these objects. They are probably virus particles. Can any one identify } them? } } http://upload.users.uth.gr/files/virus.jpg } } Many thanks } Panos } -- } Dr. Berillis Panagiotis } Lecturer in Microscopy, image analysis and histology } Department of Ichthyology and Aquatic Environment } Agricultural school } University of Thessaly } } } ==============================Original Headers============================== } 6, 18 -- From pveril-at-apae.uth.gr Thu Jan 23 04:23:12 2014 } 6, 18 -- Received: from kerberos.uth.gr (mx2.uth.gr [194.177.200.3]) } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NANBUa024847 } 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 04:23:12 -0600 } 6, 18 -- Received: from localhost (webmail.uth.gr [194.177.200.14]) } 6, 18 -- by kerberos.uth.gr (8.13.8/8.13.8) with ESMTP id s0NANwCR031164 } 6, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 12:23:58 +0200 } 6, 18 -- Received: from 10.64.60.150 ([10.64.60.150]) by webmail.uth.gr (Horde } 6, 18 -- Framework) with HTTP; Thu, 23 Jan 2014 12:23:00 +0200 } 6, 18 -- Date: Thu, 23 Jan 2014 12:23:00 +0200 } 6, 18 -- Message-ID: {20140123122300.Horde.--gLtdj-JhwWKO_juLlJ4w3-at-webmail.uth.gr} } 6, 18 -- From: Panagiotis BERILLIS {pveril-at-apae.uth.gr} } 6, 18 -- To: Microscopy-at-microscopy.com } 6, 18 -- Subject: Virus } 6, 18 -- User-Agent: Internet Messaging Program (IMP) H5 (6.0.4) } 6, 18 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes } 6, 18 -- MIME-Version: 1.0 } 6, 18 -- Content-Disposition: inline } ==============================End of - Headers============================== } }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 23 -- From tina-at-pbrc.hawaii.edu Thu Jan 23 12:46:43 2014 5, 23 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0NIkh1E020397 5, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jan 2014 12:46:43 -0600 5, 23 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 23 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id s0NIjs4K020773 5, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 23 -- Thu, 23 Jan 2014 08:45:54 -1000 (HST) 5, 23 -- Received: from localhost (tina-at-localhost) 5, 23 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id s0NIjo2Q020738; 5, 23 -- Thu, 23 Jan 2014 08:45:54 -1000 (HST) 5, 23 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 5, 23 -- Date: Thu, 23 Jan 2014 08:45:50 -1000 (HST) 5, 23 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 23 -- X-X-Sender: tina-at-sf-v240 5, 23 -- To: pveril-at-apae.uth.gr 5, 23 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 23 -- Subject: Re: [Microscopy] Virus 5, 23 -- In-Reply-To: {201401231025.s0NAPGdU025978-at-ns.microscopy.com} 5, 23 -- Message-ID: {Pine.GSO.4.64.1401230841250.19887-at-sf-v240} 5, 23 -- References: {201401231025.s0NAPGdU025978-at-ns.microscopy.com} 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Yes, pollen was my immediate thought, but pollen would be at least one, probably two, order/s of magnitude too big!
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 24/01/14 4:02 AM, "eikonika-at-otenet.gr" {eikonika-at-otenet.gr} wrote:
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We agree with those people who think that the "bubbles" have to do with something extracted from the bacteria that prevents the resin from polymerising.
We did not use osmium, we dehydrated in ethanol up to 80% ethanol and did not degas the resin. We used a premix resin that works OK with any other specimens except bacteria. But we do not add the extra accelerator.
Perhaps the next steps will be: (i) to dehydrate up to 90 % alcohol (ii) degas the resin (iii) add extra accelerator so that whatever chemical reaction we have might not occur? Any suggestions are welcome and thank you again!
Stefan
==============================Original Headers============================== 5, 30 -- From stefan.wernitznig-at-medunigraz.at Fri Jan 24 04:43:33 2014 5, 30 -- Received: from si068.medunigraz.at (si083.medunigraz.at [193.170.105.83]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0OAhW8L003061 5, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 04:43:33 -0600 5, 30 -- X-AuditID: 0ac80c44-f79eb6d000000e72-57-52e243d3360a 5, 30 -- Received: from si062.medunigraz.at (Unknown_Domain [10.200.18.79]) 5, 30 -- by si068.medunigraz.at (Symantec Mail Security) with SMTP id D2.BC.03698.3D342E25; Fri, 24 Jan 2014 11:43:31 +0100 (CET) 5, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at [10.200.112.128]) 5, 30 -- by si062.medunigraz.at with ESMTP (TLS encrypted); Fri, 24 Jan 2014 11:43:05 +0100 5, 30 -- Message-ID: {52E243B9.3030209-at-medunigraz.at} 5, 30 -- Date: Fri, 24 Jan 2014 11:43:05 +0100 5, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} 5, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 5, 30 -- MIME-Version: 1.0 5, 30 -- To: Microscopy-at-microscopy.com 5, 30 -- Subject: Re: Strange bubbles in LR white resin 5, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 5, 30 -- Content-Transfer-Encoding: 7bit 5, 30 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFlrDIsWRmVeSWpSXmKPExsXCdULIX/ey86Mgg6N9bBZ/DjewOzB6fOx+ 5, 30 -- zRTAGMVlk5Kak1mWWqRvl8CVse7KcfaCJawVDU9usjYwTmPpYuTkkBAwkZiw+SQbhC0mceHe 5, 30 -- eiCbi0NIYC+jxKOmd4wQzjxGiU9rLjGBVPEK6Eg0vOgAs1kEVCU2N1xlBLHZBCwljnf/AJsq 5, 30 -- KhAssfvxM1aIekGJkzOfgMVFBGQlFrw/wg5iCwvoSdxqugI2h1nARuLviUUsELa8xPa3c5gn 5, 30 -- MPLOQtI+C0nZLCRlCxiZVzEKF2camFno5aamlOZlphclVukllmxiBAcOj8sOxoYthocYBTgY 5, 30 -- lXh46y0fBQmxJpYVV+YeYpTkYFIS5X3qABTiS8pPqcxILM6ILyrNSS0+xCjBwawkwlt/7WGQ 5, 30 -- EG9KYmVValE+TEqag0VJnHeLxN0gIYH0xJLU7NTUgtQimKwMB4eSBG+/E9BQwaLU9NSKtMyc 5, 30 -- EoQ0EwcnyHAeoOGXQGp4iwsSc4sz0yHypxglpcR5w0ASAiCJjNI8uN5XjOJALwjzvgDJ8gCT 5, 30 -- AFzXK6CBTEADV5x9ADKwJBEhJdXA6GD0s//epWpOoYWpBd0HKoK/vJb6e3K6zp+pYlX7+GwK 5, 30 -- hQ7EX6j3fsc0I9DggzhbsepUI601LNlveU4++937Yc/VaYnK7FE3Wdv/HPM9fyKIUTbNesIG 5, 30 -- Ec3zR+TEvH3Wnyytnv4mRsQt1+SVnJ3YDcVDxj8LJriYMeT/mz75u6/4Oc18YR95JZbijERD 5, 30 -- Leai4kQAR3Z+l78CAAA= ==============================End of - Headers==============================
Since bacteria are usually plentiful and inexpensive, I would also do one sample after 100% ethanol to see if it is do to residual water. Degassing the resin can change composition since you can differentially extract the more volatile components. Tom Phillips
Sent from my iPad
} On Jan 24, 2014, at 4:44 AM, "stefan.wernitznig-at-medunigraz.at" {stefan.wernitznig-at-medunigraz.at} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thank you for the intense & helpful discussion! } } We agree with those people who think that the "bubbles" have to do with } something extracted from the bacteria that prevents the resin from } polymerising. } } We did not use osmium, we dehydrated in ethanol up to 80% ethanol and } did not degas the resin. We used a premix resin that works OK with any } other specimens except bacteria. But we do not add the extra accelerator. } } Perhaps the next steps will be: } (i) to dehydrate up to 90 % alcohol } (ii) degas the resin } (iii) add extra accelerator so that whatever chemical reaction we have } might not occur? } Any suggestions are welcome and thank you again! } } Stefan } } ==============================Original Headers============================== } 5, 30 -- From stefan.wernitznig-at-medunigraz.at Fri Jan 24 04:43:33 2014 } 5, 30 -- Received: from si068.medunigraz.at (si083.medunigraz.at [193.170.105.83]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0OAhW8L003061 } 5, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 04:43:33 -0600 } 5, 30 -- X-AuditID: 0ac80c44-f79eb6d000000e72-57-52e243d3360a } 5, 30 -- Received: from si062.medunigraz.at (Unknown_Domain [10.200.18.79]) } 5, 30 -- by si068.medunigraz.at (Symantec Mail Security) with SMTP id D2.BC.03698.3D342E25; Fri, 24 Jan 2014 11:43:31 +0100 (CET) } 5, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at [10.200.112.128]) } 5, 30 -- by si062.medunigraz.at with ESMTP (TLS encrypted); Fri, 24 Jan 2014 11:43:05 +0100 } 5, 30 -- Message-ID: {52E243B9.3030209-at-medunigraz.at} } 5, 30 -- Date: Fri, 24 Jan 2014 11:43:05 +0100 } 5, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} } 5, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 } 5, 30 -- MIME-Version: 1.0 } 5, 30 -- To: Microscopy-at-microscopy.com } 5, 30 -- Subject: Re: Strange bubbles in LR white resin } 5, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 5, 30 -- Content-Transfer-Encoding: 7bit } 5, 30 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFlrDIsWRmVeSWpSXmKPExsXCdULIX/ey86Mgg6N9bBZ/DjewOzB6fOx+ } 5, 30 -- zRTAGMVlk5Kak1mWWqRvl8CVse7KcfaCJawVDU9usjYwTmPpYuTkkBAwkZiw+SQbhC0mceHe } 5, 30 -- eiCbi0NIYC+jxKOmd4wQzjxGiU9rLjGBVPEK6Eg0vOgAs1kEVCU2N1xlBLHZBCwljnf/AJsq } 5, 30 -- KhAssfvxM1aIekGJkzOfgMVFBGQlFrw/wg5iCwvoSdxqugI2h1nARuLviUUsELa8xPa3c5gn } 5, 30 -- MPLOQtI+C0nZLCRlCxiZVzEKF2camFno5aamlOZlphclVukllmxiBAcOj8sOxoYthocYBTgY } 5, 30 -- lXh46y0fBQmxJpYVV+YeYpTkYFIS5X3qABTiS8pPqcxILM6ILyrNSS0+xCjBwawkwlt/7WGQ } 5, 30 -- EG9KYmVValE+TEqag0VJnHeLxN0gIYH0xJLU7NTUgtQimKwMB4eSBG+/E9BQwaLU9NSKtMyc } 5, 30 -- EoQ0EwcnyHAeoOGXQGp4iwsSc4sz0yHypxglpcR5w0ASAiCJjNI8uN5XjOJALwjzvgDJ8gCT } 5, 30 -- AFzXK6CBTEADV5x9ADKwJBEhJdXA6GD0s//epWpOoYWpBd0HKoK/vJb6e3K6zp+pYlX7+GwK } 5, 30 -- hQ7EX6j3fsc0I9DggzhbsepUI601LNlveU4++937Yc/VaYnK7FE3Wdv/HPM9fyKIUTbNesIG } 5, 30 -- Ec3zR+TEvH3Wnyytnv4mRsQt1+SVnJ3YDcVDxj8LJriYMeT/mz75u6/4Oc18YR95JZbijERD } 5, 30 -- Leai4kQAR3Z+l78CAAA= } ==============================End of - Headers==============================
==============================Original Headers============================== 4, 30 -- From PhillipsT-at-missouri.edu Fri Jan 24 07:35:00 2014 4, 30 -- Received: from mst-rip5-missouri-out.um.umsystem.edu (mst-rip5-missouri-out.um.umsystem.edu [198.209.50.135]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0ODYxOo023666 4, 30 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:34:59 -0600 4, 30 -- X-IronPort-Anti-Spam-Filtered: true 4, 30 -- X-IronPort-Anti-Spam-Result: Ag4FAF9r4lLPoJ7U/2dsb2JhbABagww4qkiSRIELFnSCJQEBAQMBOhQwCwIBCBEDAQIBDBIQMQEXAQUIAgQBCQkdBIdcCA3DFYR/F447AQEcCC0FBoMegRQEmVmQbIMtPIE1 4, 30 -- X-IPAS-Result: Ag4FAF9r4lLPoJ7U/2dsb2JhbABagww4qkiSRIELFnSCJQEBAQMBOhQwCwIBCBEDAQIBDBIQMQEXAQUIAgQBCQkdBIdcCA3DFYR/F447AQEcCC0FBoMegRQEmVmQbIMtPIE1 4, 30 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 4, 30 -- by mst-rip5-exch-relay.um.umsystem.edu with ESMTP; 24 Jan 2014 07:34:58 -0600 4, 30 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.204]) by 4, 30 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0169.001; 4, 30 -- Fri, 24 Jan 2014 07:34:58 -0600 4, 30 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 4, 30 -- To: " {stefan.wernitznig-at-medunigraz.at} " {stefan.wernitznig-at-medunigraz.at} , 4, 30 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 30 -- Subject: Re: [Microscopy] Re: Strange bubbles in LR white resin 4, 30 -- Thread-Topic: [Microscopy] Re: Strange bubbles in LR white resin 4, 30 -- Thread-Index: AQHPGPFAH2FL5R+ITUCOPheQKH+i8pqT4ENp 4, 30 -- Date: Fri, 24 Jan 2014 13:34:58 +0000 4, 30 -- Message-ID: {39C2E286-2C85-4550-999C-DF01A748F01A-at-missouri.edu} 4, 30 -- References: {201401241044.s0OAiOuv003904-at-ns.microscopy.com} 4, 30 -- In-Reply-To: {201401241044.s0OAiOuv003904-at-ns.microscopy.com} 4, 30 -- Accept-Language: en-US 4, 30 -- Content-Language: en-US 4, 30 -- X-MS-Has-Attach: 4, 30 -- X-MS-TNEF-Correlator: 4, 30 -- Content-Type: text/plain; charset="us-ascii" 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Transfer-Encoding: 8bit 4, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0ODYxOo023666 ==============================End of - Headers==============================
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] TEM Diffraction of amphibole fibers and fiber bundles
Message: Question for any Mineralogist-Microscopist (or the other way 'round :) ) in the group.
I have a Mineralogy student working in my lab on the TEM trying to obtain diffraction patterns (SAED) on amphibole fibers and fiber bundles (note: some of the specimens may be cleavage fragments). Particle size: 50-100 microns short dimension & 100s if microns in the long D. Scope JEOL 2010 at 200kV.
We are having a surprising difficult time getting any reasonable SAED patterns on these. We can get reasonable and nice patterns on adjacent micas in the sample but nothing of use on the amphiboles. Different spot sizes, SAED apertures, camera lengths, etc. No joy.
We should be able to do this-even though I am not the worlds best at electron diffraction?? I'm at a loss and throwing myself Forrest Gump like asking for mercy!
Suggestions would be welcome.
Thanks-Tom
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 24 07:41:08 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0ODf87s031166 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:41:08 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id E737C6AED43 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:41:08 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id 9AmVeM06qm3E for {microscopy-at-microscopy.com} ; 19, 28 -- Fri, 24 Jan 2014 07:41:07 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id B5E306AED31 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:41:07 -0600 (CST) 19, 28 -- Message-ID: {52E26D73.4030707-at-microscopy.com} 19, 28 -- Date: Fri, 24 Jan 2014 07:41:07 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:TEM Diffraction of amphibole fibers and fiber bundles 19, 28 -- References: {201401231858.s0NIwMiT006807-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201401231858.s0NIwMiT006807-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201401231858.s0NIwMiT006807-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Tom, 50-100 µm is very thick for SAED. You are probably not getting the electrons through the sample, whereas, the micas are probably thinner. The thing to try is getting diffraction patterns from the very thinnest edges of the fibers. You could crush the sample further, but that would destroy the measurement of length-width aspect. If you have EDX, you can correlate composition of the crushed sample patterns to as-received samples. Hope this helps. Ken
|_|"|_|"|_|_|"|_|"|_|"|_|_|"|_|"|_|_|"|_|"|_|"|_|_| Kenneth JT Livi, PhD Director, The High-Resolution Analytical Electron Microbeam Facility of the Integrated Imaging Center Departments of Earth and Planetary Sciences and Biology Olin Hall 3400 N Charles Street Johns Hopkins University Baltimore, Maryland 21218 USA | | | .| | .| | .| | .| | | :| | | .| | .| | .| | .| | | :|
==============================Original Headers============================== 11, 19 -- From prvs=0942b11ae=klivi-at-jhu.edu Fri Jan 24 07:53:57 2014 11, 19 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.150]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0ODrv7U023753 11, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jan 2014 07:53:57 -0600 11, 19 -- X-IronPort-AV: E=Sophos;i="4.95,712,1384318800"; 11, 19 -- d="scan'208";a="21952747" 11, 19 -- Received: from unknown (HELO [128.220.60.54]) ([128.220.60.54]) 11, 19 -- by ipex0.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 24 Jan 2014 08:53:53 -0500 11, 19 -- From: Ken Livi {klivi-at-jhu.edu} 11, 19 -- Content-Type: text/plain; charset=iso-8859-1 11, 19 -- Subject: [Microscopy] viaWWW:TEM Diffraction of amphibole fibers and fiber bundles 11, 19 -- Date: Fri, 24 Jan 2014 08:53:52 -0500 11, 19 -- Message-Id: {3BB2F6B4-3AC5-443D-A34E-B9E684018256-at-jhu.edu} 11, 19 -- Cc: Microscopy-at-microscopy.com 11, 19 -- To: tomw-at-uidaho.edu 11, 19 -- Mime-Version: 1.0 (Apple Message framework v1283) 11, 19 -- X-Mailer: Apple Mail (2.1283) 11, 19 -- Content-Transfer-Encoding: 8bit 11, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0ODrv7U023753 ==============================End of - Headers==============================
the samples are thin enough for TEM? Your description sounds a lot like the fibers are too thick, they need to be less than half a micron thick to allow you a chance to get results. Cleavage is a great technique but you might need to do it twenty times to get something decent.
Good luck Rob
-- Robert Keyse, DPhil Research Scientist Lehigh University
5, East Packer Avenue, Whitaker Laboratory Bethlehem, PA 18015-3194
Office (610)758-3465 Fax (610)758-4244
==============================Original Headers============================== 7, 21 -- From rok210-at-lehigh.edu Fri Jan 24 08:03:18 2014 7, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0OE3IZT005256 7, 21 -- for {Microscopy-at-Microscopy.com} ; Fri, 24 Jan 2014 08:03:18 -0600 7, 21 -- Received: from [127.0.0.1] (Dyn055015.mat.Lehigh.EDU [128.180.55.15]) 7, 21 -- (authenticated bits=0) 7, 21 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id s0OE3HCQ009183 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 7, 21 -- Fri, 24 Jan 2014 09:03:18 -0500 7, 21 -- Message-ID: {52E272A5.5030207-at-lehigh.edu} 7, 21 -- Date: Fri, 24 Jan 2014 09:03:17 -0500 7, 21 -- From: Robert Keyse {rok210-at-lehigh.edu} 7, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 7, 21 -- MIME-Version: 1.0 7, 21 -- To: Microscopy-at-Microscopy.com 7, 21 -- CC: tomw-at-uidaho.edu 7, 21 -- Subject: TEM Diffraction of amphibole fibers and fiber bundles 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Virus-Scanned: clamav-milter 0.98 at rain.cc.lehigh.edu 7, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I do not remember seeing how long your steps were during dehydration in ethanol but do know that these little bacteria take longer than anticipated to dehydrate. I have also found it advantageous to have the ethanol moving by putting the tubes on a tilting table or to rotate them during dehydration and also for the infiltration steps.
I have had similar looking bacteria in Macrophages embedded in EPON in the past. After the first paper was published I learned that my problem was their dehydration. The macrophages themselves looked fine when processed by my standard tissue culture protocol but the Listeria within them turned very dark and peppered like yours when the beam hit them. After I started to use our longer protocol for tissue samples, the bacteria looked great.
Many years ago I was advised to go through 90% ethanol when using LR White so your suggestion to do that is a good one.
One can introduce air/oxygen into the embedding media when stirring in the accelerator so degassing could help in the elimination of that possibility.
I usually harden the LR White in a 45 degree C. oven for a few days without accelerator so I can not address the possibility of adding an additional amount of accelerator.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Pat
Patricia Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-nhlbi.nih.gov
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In bright field look for areas that exhibit bend contours. Those are the areas that are thin enough where you can find a reasonable pattern usually at the terminations.
Find fibers that are elongated in the direction of the tilt axis, adjust the height of the sample so you can tilt without the fiber moving.
Tilting about the long axis which is [001] you can see the closely spaced spots of 010.
Tilting about 010, ideally you should find [100] and [101] about 30° apart.
Easy to find in the orthorhombic amphiboles and less easy in the monoclinic amphiboles.
I suggest practicing with iucc standards. Tremolite and anthophyllite.
Also with Dave Palmer's Single Crystal program you don't need a TEM.
Getting indexable diffraction patterns off fibrous amphiboles requires patience which is why asbestos labs don't do it.
Gordon --------------------------------------------------- Gordon L. Nord, Ph. D Senior Scientist International Environmental Research Foundation P.O. Box 3459, Grand Central Station New York, NY 10163-3459
{http://www.ierfinc.org/}
==============================Original Headers============================== 17, 25 -- From gnord-at-mindspring.com Fri Jan 24 13:45:11 2014 17, 25 -- Received: from elasmtp-dupuy.atl.sa.earthlink.net (elasmtp-dupuy.atl.sa.earthlink.net [209.86.89.62]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0OJjBTo024549 17, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Jan 2014 13:45:11 -0600 17, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 17, 25 -- s=dk20050327; d=mindspring.com; 17, 25 -- b=OSWu1aeZGRUOdW3AAquhk8Vm8my/3TybTvBaKzLA6+ILxVddTOmwEYjeoL2n3Di8; 17, 25 -- h=Received:From:Content-Type:Content-Transfer-Encoding:Subject:Date:Message-Id:To:Mime-Version:X-Mailer:X-ELNK-Trace:X-Originating-IP; 17, 25 -- Received: from [96.247.192.239] (helo=earth.home) 17, 25 -- by elasmtp-dupuy.atl.sa.earthlink.net with esmtpa (Exim 4.67) 17, 25 -- (envelope-from {gnord-at-mindspring.com} ) 17, 25 -- id 1W6mgW-0003le-Os 17, 25 -- for Microscopy-at-Microscopy.Com; Fri, 24 Jan 2014 14:45:00 -0500 17, 25 -- From: Gordon Nord {gnord-at-mindspring.com} 17, 25 -- Content-Type: text/plain; charset=iso-8859-1 17, 25 -- Subject: Re: [Microscopy] viaWWW:TEM Diffraction of amphibole fibers and fiber bundles 17, 25 -- Date: Fri, 24 Jan 2014 14:44:59 -0500 17, 25 -- Message-Id: {0A8C9829-63C3-4296-BDE3-DE92A1466AF8-at-mindspring.com} 17, 25 -- To: Microscopy-at-Microscopy.Com 17, 25 -- Mime-Version: 1.0 (Apple Message framework v1085) 17, 25 -- X-Mailer: Apple Mail (2.1085) 17, 25 -- X-ELNK-Trace: 3df33169c923931ad4c20f6b8d69d88891b749bef7332bbc58b6c049e7d0e0da6c68a24449faf2cf350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 17, 25 -- X-Originating-IP: 96.247.192.239 17, 25 -- Content-Transfer-Encoding: 8bit 17, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0OJjBTo024549 ==============================End of - Headers==============================
The objects you have seen under your EM are not virus or pollen, They are Brochosomes. Brochosomes are secretory granules produced by the Malphigian tubules of leafhoppers. You may have a look at the Wikipedia article concerning this unfamiliar but beautiful structure (wikipedia.org/wiki/Brochosome) . I have seen EM pictures of this structure many years ago , since it has been once described in the sixties by my former colleagues in the lab in Rennes (France, Brittany). (Gouranton J. & Maillet P.L. (1967) Origine et structure des brochosomes. Journal de Microscopie 6: 53-64.). Brochosomes are source of contaminations and can sometimes be found in aerosol (Wiffen R. D. & Heard M. J. (1969) Unidentified airborn species. Nature 224: 715). I recommend the readings associated with the Wiki article. However, if you are interested, I can look over the archives of the lab and try to find original negatives.
Daniel
Daniel THOMAS UMR CNRS 6290 Universite de Rennes 1 35042 RENNES, FRANCE
==============================Original Headers============================== 4, 17 -- From daniel.thomas-at-univ-rennes1.fr Sat Jan 25 05:46:16 2014 4, 17 -- Received: from smtp.smtpout.orange.fr (smtp05.smtpout.orange.fr [80.12.242.127]) 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0PBkFNJ002358 4, 17 -- for {microscopy-at-microscopy.com} ; Sat, 25 Jan 2014 05:46:15 -0600 4, 17 -- Received: from air-de-daniel.home ([2.10.186.254]) 4, 17 -- by mwinf5d10 with ME 4, 17 -- id JBmE1n0025VlCY203BmExC; Sat, 25 Jan 2014 12:46:14 +0100 4, 17 -- From: Daniel Thomas {daniel.thomas-at-univ-rennes1.fr} 4, 17 -- Content-Type: text/plain; charset=us-ascii 4, 17 -- Subject: EM Virus 4, 17 -- Message-Id: {4A7927F1-9876-462E-BFCE-E4042BF2F08B-at-univ-rennes1.fr} 4, 17 -- Date: Sat, 25 Jan 2014 12:46:13 +0100 4, 17 -- To: microscopy-at-microscopy.com 4, 17 -- Mime-Version: 1.0 (Mac OS X Mail 7.1 \(1827\)) 4, 17 -- X-Mailer: Apple Mail (2.1827) 4, 17 -- Content-Transfer-Encoding: 8bit 4, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0PBkFNJ002358 ==============================End of - Headers==============================
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Merci Daniel for showing these brochosomes, they have a very smart shape. A pattern repeated in many structures seen at different scales in this wonderful world. And I am sure you gave the right answer.
----- Original Message ----- X-from: {daniel.thomas-at-univ-rennes1.fr} To: {eikonika-at-otenet.gr} Sent: Saturday, January 25, 2014 1:55 PM
March 24-28, 2014 a course in scanning electron microscopy will be offered at Hooke College of Applied Sciences. Further information about this hands-on course can be found at the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax) www.hookecollege.com
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From medications_canadian7-at-cyfrowypolsat.pl Tue Jan 28 11:58:16 2014 Return-Path: {medications_canadian7-at-cyfrowypolsat.pl} Received: from ip-37-109-125-139.multi.internet.cyfrowypolsat.pl (ip-37-109-125-139.multi.internet.cyfrowypolsat.pl [37.109.125.139]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0SHwAJp018396 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 28 Jan 2014 11:58:13 -0600 Message-Id: {201401281758.s0SHwAJp018396-at-ns.microscopy.com}
We have an old Zygo optical profilometer that we are looking to get rid of. The microscope works fine but the computer to which it is attached is not operational as no one remembers the root password and we don't have the operating system tapes to re-install from, it runs HP Unix. I have taken some photos and they can be viewed at;
Again the item is in Minneapolis, MN and it is free but the buyer must assume all shipping costs.
Nick Seaton University of Minnesota Characterization Facility
--
Dr Nicholas Seaton
SEM Specialist Characterization Facility
College of Science and Engineering
University of Minnesota
Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
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From pharmacy_reasonable7-at-kidsforsavingearth.org Wed Jan 29 01:07:16 2014 Return-Path: {pharmacy_reasonable7-at-kidsforsavingearth.org} Received: from mail3.kidsforsavingearth.org (46.191.166.37.dynamic.ufanet.ru [46.191.166.37] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0T779rp019831 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 29 Jan 2014 01:07:11 -0600 Message-Id: {201401290707.s0T779rp019831-at-ns.microscopy.com}
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Role of CaCl2 in biological sample fixation for TEM
Message: What is the role of CaCl2 in biological sample fixation for TEM.? What is the recommended concentration required in phosphate buffer.?
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Ravi, Calcium and magnesium chloride are used in TEM fixatives to protect membranes from enzymes that are still active during fixation. This is refered to as maintaining membrane tonicity (small breaks in membranes may occur). If phosphate buffers are used, you must use only MgCl2 (3 mM), as calcium will precipitate to calcium phosphate.
sincerely, Michael Delannoy Johns Hopkins SOM Microscope Facility
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X-from: joseph.n.madary.civ-at-mail.mil ()
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Email: joseph.n.madary.civ-at-mail.mil Name: Nick Madary
Message: Has anyone out there actually seen how the diamond knives are resharpened? I wonder if there is a tool and a training to attempt it. We have so many and it is difficult to buy them for us. Anyone also know of a compnmay that takes several knives in exchange for a knife? Like giving up 3-5 knives for a new one?
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==============================Original Headers============================== 10, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 29 13:47:57 2014 10, 28 -- Received: from znl.com ([206.69.208.20]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0TJlv0d007832 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jan 2014 13:47:57 -0600 10, 28 -- Received: from localhost (localhost [127.0.0.1]) 10, 28 -- by znl.com (Postfix) with ESMTP id B8ECA6B24ED 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jan 2014 13:47:57 -0600 (CST) 10, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 28 -- Received: from znl.com ([127.0.0.1]) 10, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id DqKf3XFmhRUn for {microscopy-at-microscopy.com} ; 10, 28 -- Wed, 29 Jan 2014 13:47:56 -0600 (CST) 10, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 10, 28 -- by znl.com (Postfix) with ESMTPA id 58C246B24E0 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jan 2014 13:47:56 -0600 (CST) 10, 28 -- Message-ID: {52E95AEC.9050101-at-microscopy.com} 10, 28 -- Date: Wed, 29 Jan 2014 13:47:56 -0600 10, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 10, 28 -- MIME-Version: 1.0 10, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 28 -- Subject: viaWWW:Diamond knife sharpening 10, 28 -- References: {201401291801.s0TI1eCA006638-at-ns.microscopy.com} 10, 28 -- In-Reply-To: {201401291801.s0TI1eCA006638-at-ns.microscopy.com} 10, 28 -- X-Forwarded-Message-Id: {201401291801.s0TI1eCA006638-at-ns.microscopy.com} 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Working on an issue that has come up in my Leo 1450VPSE SEM since I changed a gun filament. Seeing light and dark scan lines in captured images. First thought it's some kind of charging behavior but not sure. Problem occurs with both SE and BS detectors as I try to capture an image using the LINE AVG mode where a line of the image is scanned a number of times (N) before moving to the next line. This builds my image one scan line at a time till I have a full frame. Using scan speed six (rate it scans frame) I get different widths of shadow line as I change N.
Since I cannot attach images, I can Email them direct to anyone that may want to offer a suggestion as to what is causing the problem.
Thanks
Roy Beavers Southern Methodist University rbeavers-at-smu.edu
==============================Original Headers============================== 6, 28 -- From rbeavers-at-mail.smu.edu Wed Jan 29 14:00:04 2014 6, 28 -- Received: from smtap2.systems.smu.edu (smtap2.systems.smu.edu [129.119.65.151]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0TK04ig023009 6, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Jan 2014 14:00:04 -0600 6, 28 -- X-IronPort-Anti-Spam-Filtered: true 6, 28 -- X-IronPort-Anti-Spam-Result: AgsFAGRc6VKBd0GG/2dsb2JhbABZgmshgQ69DIEHFnSCJwU6UQEqFEImAQQTCId9AZtPpxCHMxeOHREBH4NcgRQEqkeDLYFxOQ 6, 28 -- X-IPAS-Result: AgsFAGRc6VKBd0GG/2dsb2JhbABZgmshgQ69DIEHFnSCJwU6UQEqFEImAQQTCId9AZtPpxCHMxeOHREBH4NcgRQEqkeDLYFxOQ 6, 28 -- Received: from sxht1p3.systems.smu.edu ([129.119.65.134]) 6, 28 -- by smtah2.systems.smu.edu with ESMTP/TLS/AES128-SHA; 29 Jan 2014 14:00:04 -0600 6, 28 -- Received: from SXMB1PG.SYSTEMS.SMU.EDU ([fe80::1449:c186:5adb:ddbb]) by 6, 28 -- SXHT1P3.SYSTEMS.SMU.EDU ([fe80::4479:3bff:ca05:ebce%17]) with mapi id 6, 28 -- 14.02.0387.000; Wed, 29 Jan 2014 14:00:03 -0600 6, 28 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 6, 28 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 28 -- Subject: Help with scan line problem in Leo 1450VPSE 6, 28 -- Thread-Topic: Help with scan line problem in Leo 1450VPSE 6, 28 -- Thread-Index: Ac8dLIfYOqs5okgrTjuz906249AkOQ== 6, 28 -- Date: Wed, 29 Jan 2014 20:00:03 +0000 6, 28 -- Message-ID: {BC0C36E74D341741A25CE2ABB122E7A330AF551D-at-SXMB1PG.SYSTEMS.SMU.EDU} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- x-originating-ip: [129.119.65.139] 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0TK04ig023009 ==============================End of - Headers==============================
Nick, Diatome diamond knives will replace a used knife (any size) for a brand new knife (any size) at half the cost of a new knife. Believe me, this is the best deal I know of. If you find a better one, let us all know.
sincerely, Michael Delannoy Johns Hopkins SOM Microscope Facility
----- Original Message ----- X-from: microscopylistserver-noreply-at-microscopy.com
Nick,
Years ago, a knife vendor gave a talk on how diamond knives are sharpened. It is not hard, but takes 1-2 weeks of grinding on a diamond grinding wheel. This in not something that you can do in your leisure. I once asked another vendor this same question about giving them 3 or four knives and get a new sharpened knife in return. After picking themselves off the ground from laughing so hard, they explained to me that the diamond is about 40-50 dollars, it is the time it takes to sharpen the knife that cost so much, so they will not just give a sharpened knife with exchange of 3 or 4 knives. One of the vendors does give a discount for a 2 for 1 exchange. Call them up and ask about this.
Best of luck convincing the powers that be to purchase a knife for you.
Ed
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, January 29, 2014 2:52 PM To: Calomeni, Edward
X-from: duraine-at-bcm.edu ()
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Message: Diatome has a deal where if you send in three knives for re-sharpening, they will charge for two of them at the re-sharpen price and the third one is free.
Since you have many knives it might be advantageous to keep a few sets of three so when you send one set in you will have another to work with.
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From most answers - especially Pat's answer - I gather that dehydration is important, so our next test will be whether dehydrating to 90 or 100 % ethanol improves the structure and gets rid of the "bubbles". The alcohol steps were for 30 min each with an overnight break in 70 % alcohol so I do not think longer incubation times will help.
We use LR white because we want to perform immunolabelling, so I think we have to find the right balance between structural preservation and preservation of antigenicity. Our next approach will be with dehydration up to 80, 90, and 100 % ethanol in parallel. Thank you again!
Stefan
==============================Original Headers============================== 4, 30 -- From stefan.wernitznig-at-medunigraz.at Thu Jan 30 07:44:47 2014 4, 30 -- Received: from si068.medunigraz.at (si083.medunigraz.at [193.170.105.83]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0UDikUu024957 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 07:44:47 -0600 4, 30 -- X-AuditID: 0ac80c44-f79eb6d000000e72-fc-52ea574c926c 4, 30 -- Received: from si062.medunigraz.at (Unknown_Domain [10.200.18.79]) 4, 30 -- by si068.medunigraz.at (Symantec Mail Security) with SMTP id 52.93.03698.C475AE25; Thu, 30 Jan 2014 14:44:44 +0100 (CET) 4, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at [10.200.112.128]) 4, 30 -- by si062.medunigraz.at with ESMTP (TLS encrypted); Thu, 30 Jan 2014 14:44:41 +0100 4, 30 -- Message-ID: {52EA574A.3080400-at-medunigraz.at} 4, 30 -- Date: Thu, 30 Jan 2014 14:44:42 +0100 4, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} 4, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 4, 30 -- MIME-Version: 1.0 4, 30 -- To: Microscopy-at-microscopy.com 4, 30 -- Subject: Re: Strange Bubbles in LR white resin 4, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 4, 30 -- Content-Transfer-Encoding: 7bit 4, 30 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFlrJIsWRmVeSWpSXmKPExsXCdULIX9cn/FWQwa4ZEhZ/DjewOzB6fOx+ 4, 30 -- zRTAGMVlk5Kak1mWWqRvl8CV0fNrGnvBWtaKFSfvsTcwzmPpYuTkkBAwkVh/uwnKFpO4cG89 4, 30 -- G4gtJLCXUWJXK18XIxeQPY9RYuvkX0AJDg5eAR2JeQ9YQEwWAVWJk4vzQMrZBCwljnf/ABsj 4, 30 -- KhAssfvxM1YQm1dAUOLkzCdgcREBWYkF74+wg9jCAnoSZ+btZwSxmQVsJP6eWMQCYctLbH87 4, 30 -- h3kCI+8sJO2zkJTNQlK2gJF5FaNwcaaBmYVebmpKaV5melFilV5iySZGcMjwuOxgbNhieIhR 4, 30 -- gINRiYdXwPtVkBBrYllxZe4hRkkOJiVR3sRgoBBfUn5KZUZicUZ8UWlOavEhRgkOZiURXg1N 4, 30 -- oBxvSmJlVWpRPkxKmoNFSZy3IupZkJBAemJJanZqakFqEUxWhoNDSYL3YyhQo2BRanpqRVpm 4, 30 -- TglCmomDE2Q4D9DwhyA1vMUFibnFmekQ+VOMklLivIZhQAkBkERGaR5c7ytGcaAXhHn/g7Tx 4, 30 -- AOMfrusV0EAmoIFiO16ADCxJREhJNTCeWTtjpsiU1S/3ruaOSZ99THeXIS/f4m/ueqLPM1bJ 4, 30 -- rq6WzIkUZdluf4JLSHRBYRZTsNbN976e8yc4/oh/k8NgGbfRZFP71N9V0n6PBEQ117EfcLKq 4, 30 -- kWU0XLVOh5vhrBH3xaBzSrP45HgOu5v3nNR51XhkhlGGWmFxZ3ZJmipv2rSFlqm2jEosxRmJ 4, 30 -- hlrMRcWJAI9kinq8AgAA ==============================End of - Headers==============================
O.k., higher ups at the University have been looking at service contracts costs and trying to
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
==============================Original Headers============================== 4, 24 -- From Edelmare-at-MiamiOH.edu Thu Jan 30 08:44:41 2014 4, 24 -- Received: from mualmaip02.mcs.muohio.edu (mualmaip02.mcs.muohio.edu [134.53.6.8]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0UEieXI012964 4, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 08:44:41 -0600 4, 24 -- X-DNSBL-MILTER: Skipped 4, 24 -- Received: from [10.34.160.232] ([10.34.160.232]) 4, 24 -- by mualmaip02.mcs.muohio.edu (8.14.4/8.14.4) with ESMTP id s0UEiVIV028748 4, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 09:44:31 -0500 4, 24 -- From: "Richard E. Edelmann" {Edelmare-at-MiamiOH.edu} 4, 24 -- To: Microscopy-at-microscopy.com 4, 24 -- Date: Thu, 30 Jan 2014 09:44:32 -0500 4, 24 -- MIME-Version: 1.0 4, 24 -- Subject: U 4, 24 -- Message-ID: {52EA6550.24426.3C0F31C-at-Edelmare.MiamiOH.edu} 4, 24 -- Priority: normal 4, 24 -- X-mailer: Pegasus Mail for Windows (4.63) 4, 24 -- Content-type: text/plain; charset=US-ASCII 4, 24 -- Content-transfer-encoding: 7BIT 4, 24 -- Content-description: Mail message body 4, 24 -- X-Miami-MailScanner-Information: Please contact the ISP for more information 4, 24 -- X-Miami-MailScanner-ID: s0UEiVIV028748 4, 24 -- X-Miami-MailScanner: Found to be clean 4, 24 -- X-Miami-MailScanner-From: edelmare-at-miamioh.edu 4, 24 -- X-Spam-Status: No ==============================End of - Headers==============================
O.k., higher ups at the University have been looking at service contracts costs and trying to find cost reductions. One the vendors they are now thinking about is Unity Asset Management Service (Part of Thermo Fisher Scientific). To provide repair services for all the equipment on campus.
I did a search of the listserv archives and did not find any thing about Unity. SO does anyone out there have any feed back with regards to "Unity Asset Management Service"? For Microscopes or anything else.
I do not want to start a war, and I know alternatoves have been disscussed before, but its new year and time to discuss a little again.
Happy to take anything offline or through the list or even a phone call.
Thank you.
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
==============================Original Headers============================== 9, 24 -- From Edelmare-at-MiamiOH.edu Thu Jan 30 08:53:41 2014 9, 24 -- Received: from mualmaip02.mcs.muohio.edu (mualmaip02.mcs.muohio.edu [134.53.6.8]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0UEreNi031747 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 08:53:41 -0600 9, 24 -- X-DNSBL-MILTER: Skipped 9, 24 -- Received: from [10.34.160.232] ([10.34.160.232]) 9, 24 -- by mualmaip02.mcs.muohio.edu (8.14.4/8.14.4) with ESMTP id s0UErZsv030414 9, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 09:53:35 -0500 9, 24 -- From: "Richard E. Edelmann" {Edelmare-at-MiamiOH.edu} 9, 24 -- To: Microscopy-at-microscopy.com 9, 24 -- Date: Thu, 30 Jan 2014 09:53:35 -0500 9, 24 -- MIME-Version: 1.0 9, 24 -- Message-ID: {52EA676F.27167.3C93E86-at-Edelmare.MiamiOH.edu} 9, 24 -- Priority: normal 9, 24 -- X-mailer: Pegasus Mail for Windows (4.63) 9, 24 -- Content-type: text/plain; charset=US-ASCII 9, 24 -- Content-transfer-encoding: 7BIT 9, 24 -- Content-description: Mail message body 9, 24 -- X-Miami-MailScanner-Information: Please contact the ISP for more information 9, 24 -- X-Miami-MailScanner-ID: s0UErZsv030414 9, 24 -- X-Miami-MailScanner: Found to be clean 9, 24 -- X-Miami-MailScanner-From: edelmare-at-miamioh.edu 9, 24 -- Subject: Unity Asset Management Service? 9, 24 -- X-Spam-Status: No ==============================End of - Headers==============================
I hope all is well. If you are interested in advancing your EELS and EFTEM (analytical TEM) knowledge, Gatan is offering a 4 day professional training school on April 8-11, 2014 at its R&D Headquarters in Pleasanton, CA, USA. Here are all the details and online registration: http://www.gatan.com/resources/training/EELSSchoolApr2014.php
-- Best regards,
John Marketing Communications Manager
Gatan, Inc.
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==============================Original Headers============================== 9, 25 -- From JHyun-at-gatan.com Thu Jan 30 12:39:43 2014 9, 25 -- Received: from email.gatan.com (email.gatan.com [50.58.128.3]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0UIdg3F022275 9, 25 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jan 2014 12:39:43 -0600 9, 25 -- Received: from MSE.gatan.com ([fe80::4968:b1cf:a686:47eb]) by email.gatan.com 9, 25 -- ([fe80::5125:aff1:b4a6:ad6e%10]) with mapi id 14.03.0174.001; Thu, 30 Jan 9, 25 -- 2014 10:39:38 -0800 9, 25 -- From: John Hyun {JHyun-at-gatan.com} 9, 25 -- To: Microscopy Server {microscopy-at-microscopy.com} 9, 25 -- Subject: EELS and EFTEM Training School 9, 25 -- Thread-Topic: EELS and EFTEM Training School 9, 25 -- Thread-Index: AQHPHeqgzKsqd/NQX0CXFEwiVH4AOw== 9, 25 -- Date: Thu, 30 Jan 2014 18:39:38 +0000 9, 25 -- Message-ID: {CF0FDB05.2F1FA%jhyun-at-gatan.com} 9, 25 -- In-Reply-To: {201401301351.s0UDpWMJ001473-at-ns.microscopy.com} 9, 25 -- Accept-Language: en-US 9, 25 -- Content-Language: en-US 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- x-originating-ip: [10.13.7.151] 9, 25 -- Content-Type: text/plain; charset="us-ascii" 9, 25 -- Content-ID: {581B1B46E3369F4D9DD2F76175E370C7-at-gatan.com} 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0UIdg3F022275 ==============================End of - Headers==============================
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dear Rick, dear all,
} O.k., higher ups at the University have been looking at service } contracts costs and trying to find cost reductions.
every year the same issue, in your institution and in many/most others as well.
from my 23 years experience, a service contract over 23 years is far better for the scopes and for all users of the scopes (i.e. for all projects!) than 23 years of waiting for the engineer(s), not knowing when (s)he will come, and who is going to pay for it. - We all know this, and most people in the community will agree - except some / many of the "Ups at the University" (nice word...), and those in the Administration.
} One the vendors they are now thinking about is Unity Asset Management } Service (Part of Thermo Fisher Scientific). To provide repair services for } all the equipment on campus.
for most of us: the service engineers of the companies are best trained on the scopes of each particular brand. Thus, a service contract for an electron microscope, and for most of the modern complex CLSM's and their variants, is "best-value-for-money", no doubt about this - in most cases. I am aware that there are other meanings around, other experiences; like: no good experience with the engineer, or too-few-engineers ... (happened to me as well, several times), or trained engineers are available / employed in the institution / University: Not here.
} I did a search of the listserv archives and did not find any thing about Unity. } SO does anyone out there have any feed back with regards to "Unity Asset } Management Service"? For Microscopes or anything else. I do not know anything about Unity, sorry.
One of my arguments. Let's take an (ordinary) car for 25.000 EUR or $ (not really comparible to our scopes, in complexity and pricing!) which is used daily, say 6 hours per day. Quickly, you will end up with an annual mileage of 20.000 km at least, if not 50.000 km (ask your service engineer, who really drive a lot!). This will raise maintenance costs for this car (oil, tyres, repairs, ...) of at least 1.000 EUR or $ per year (i.e. 4% of the initial investment), most likely even higher. Not in the first two years, but then, it will increase, quickly, dramatically. We are prepared to pay for this, because a car is what we think we need, daily. - Our students / postdocs need the scopes daily, probably more than the car (most likely), and in many institutions, the scope is far more complex and used for more hours than only 6 per day. - But, 3 to 4% of the investment for the annual maintenance contract, offering quick and expert response: this is too high, too much money? - And, compare this with the vendor of your car ... are the service engineers as good as those for the scopes? do they travel such long distances, each week / month? Richard, I am facing the same questions, every year or every second year. I still hope that we can convince the people "Up-in-the-Univ" or in the Administration.
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conference: http://www.imc2014.com/ 18th IMC 2014 in Prague, Czech Rep.
==============================Original Headers============================== 11, 27 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Fri Jan 31 02:33:50 2014 11, 27 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0V8XoI4003960 11, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 02:33:50 -0600 11, 27 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 11, 27 -- by localhost (Postfix) with SMTP id 6473A36BC5 11, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 09:33:45 +0100 (CET) 11, 27 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 11, 27 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 11, 27 -- (Client CN "gwsmtp.uni-regensburg.de", Issuer "Uni Regensburg CA - G01" (not verified)) 11, 27 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTPS id 173CF368E8 11, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 09:33:44 +0100 (CET) 11, 27 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 11, 27 -- with Novell_GroupWise; Fri, 31 Jan 2014 09:32:15 +0100 11, 27 -- Message-Id: {52EB6DF70200005400043751-at-gwsmtp1.uni-regensburg.de} 11, 27 -- X-Mailer: Novell GroupWise Internet Agent 12.0.2 11, 27 -- Date: Fri, 31 Jan 2014 09:33:43 +0100 11, 27 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 11, 27 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 11, 27 -- Subject: Unity Asset Management Service? 11, 27 -- References: {201401301453.s0UErpYo032025-at-ns.microscopy.com} 11, 27 -- In-Reply-To: {201401301453.s0UErpYo032025-at-ns.microscopy.com} 11, 27 -- Mime-Version: 1.0 11, 27 -- Content-Type: text/plain; charset=US-ASCII 11, 27 -- Content-Disposition: inline 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s0V8XoI4003960 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fxwatanabe-at-ualr.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Center for Integrative Nanotechnology Sciences UALR
Title-Subject: [Filtered] Follow up on Consolidated Service Contract inquiry
Message: Hello everyone who are concerned with similar problem of pressure to consider a consolidated service contract.
There was a post by Richard Edelmann on Unity Asset Management Service. We are being urged to consider another service called Remi (state of Arkansas has a contract with them, I've been told):
https://www.theremigroup.com/default.aspx
Our main instruments are from JEOL (TEM/SEM), Bruker (XRD and AFM), Horiba (Raman, Photoluminescence, Elipsometry), Thermo Fisher (XPS), etc. Does anybody have experience with Remi service (especially with cases involving other vendors such as JEOL, Bruker-AXS, or Thermo Fisher)? I have contacted Richard and have received reply from him already on Unity (thank you very much Richard for very quick reply). Any information on Remi would help us since we have no idea about them.
Thank you very much.
Fumiya
Fumiya Watanabe, Ph.D. Director of Instrumentation Center for Integrative Nanotechnology Sciences ETAS 153E University of Arkansas at Little Rock 2801 S. University Ave. Little Rock, AR 72204 (501) 683-7479 (Office)
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Email: pete.finger-at-jax.org Name: Pete Finger
Organization: The Jackson Laboratory
Title-Subject: [Filtered] Negative Staining Problem
Message: I’ve been doing some negative staining and am experiencing some problems with the Formvar film falling apart on the grid. I’ve been using 200 mesh Copper Formvar/carbon coated grids which are a couple of years old – does anyone know if these grids have a certain shelf life? My technique has been to put a drop of the protein (in HEPES buffer) on the grid, blot dry, add a drop of uranyl acetate, blot dry and then let the grid air dry. When I view the grid on the TEM, pretty much all the Formvar is gone. I haven’t done a lot of negative staining in the past so any comments/suggestions would be greatly appreciated. Thanks much.
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==============================Original Headers============================== 11, 39 -- From nestor.zaluzec-at-gmail.com Fri Jan 31 13:06:52 2014 11, 39 -- Received: from mail-pa0-f47.google.com (mail-pa0-f47.google.com [209.85.220.47]) 11, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0VJ6q1J031041 11, 39 -- for {microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 13:06:52 -0600 11, 39 -- Received: by mail-pa0-f47.google.com with SMTP id kp14so4741577pab.6 11, 39 -- for {microscopy-at-microscopy.com} ; Fri, 31 Jan 2014 11:06:51 -0800 (PST) 11, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 39 -- d=gmail.com; s=20120113; 11, 39 -- h=sender:message-id:date:from:reply-to:user-agent:mime-version:to 11, 39 -- :subject:references:in-reply-to:content-type 11, 39 -- :content-transfer-encoding; 11, 39 -- bh=/1o0BsXPlPcweNFouHZkzKJwGf3uN22ockP2VEwj1Yg=; 11, 39 -- b=giJT+iec0Htv/A7sUkZQO4nd4UbCRGv0IcFHgLAaz1SDH5hibTITR0c6MzX87F4tnS 11, 39 -- cqJX4aHpjAn65/3vtPyxodhC/FrcN1VH636lUms2A2bcztwERBWWXhVtzvedM3z8G9vU 11, 39 -- ++ummWJBip5BDn7tuc6rnziRtWT7mn2ITvc4jhzE4EwQ3yZ6Cu9bCxkXY9rrY541Us61 11, 39 -- HvfFdKT9InjTytZd4kVc2fu5QFI06Xr4DottK9ARBH3zV2z0D15n4ICfJkk0VRKh1X85 11, 39 -- klIL58SaGdyXGnwRV7tFrdu5THm39WlBogTpnUNNtaPn9PaYL9nAeUZf94h7kWMhEKSa 11, 39 -- Ks3g== 11, 39 -- X-Received: by 10.67.5.233 with SMTP id cp9mr22084816pad.147.1391195211735; 11, 39 -- Fri, 31 Jan 2014 11:06:51 -0800 (PST) 11, 39 -- Received: from NestorPwrBk.local ([210.9.11.226]) 11, 39 -- by mx.google.com with ESMTPSA id c7sm30091284pbt.0.2014.01.31.11.06.50 11, 39 -- for {microscopy-at-microscopy.com} 11, 39 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 11, 39 -- Fri, 31 Jan 2014 11:06:51 -0800 (PST) 11, 39 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 11, 39 -- Message-ID: {52EBF446.6040305-at-microscopy.com} 11, 39 -- Date: Fri, 31 Jan 2014 13:06:46 -0600 11, 39 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 11, 39 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 39 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 11, 39 -- MIME-Version: 1.0 11, 39 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 39 -- Subject: viaWWW:Negative Staining Problem 11, 39 -- References: {201401301419.s0UEJrkl012493-at-ns.microscopy.com} 11, 39 -- In-Reply-To: {201401301419.s0UEJrkl012493-at-ns.microscopy.com} 11, 39 -- X-Forwarded-Message-Id: {201401301419.s0UEJrkl012493-at-ns.microscopy.com} 11, 39 -- Content-Type: text/plain; charset=UTF-8; format=flowed 11, 39 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From canadian_drugs15-at-mgts.by Fri Jan 31 14:43:34 2014 Return-Path: {canadian_drugs15-at-mgts.by} Received: from mm-241-186-84-93.dynamic.pppoe.mgts.by ([178.126.52.235]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s0VKhRN9004495 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 31 Jan 2014 14:43:28 -0600 Message-Id: {201401312043.s0VKhRN9004495-at-ns.microscopy.com}
Dear all, dear Nestor, dear Fumiya,
in 2012 there was a thread on MSA-Listserver concerning the matter you are requesting:
Thread started 08.05.2012 - ended: 09.06.2012 "Remi Group as a equipmt service contract provider"
There have been several answers. For your convenience (so you would not need to got through the MSA-Listserver Archives):
Since I was interested too, I have collected these Replies in a word.docx/pdf, which I could provide easily in sending to your e-mail-box, if you allow this. Just write to me at w.muss-at-salk.at and receive - with your permission the pdf of this collection of replies.
Best wishes and regards,
Wolfgang
Wolfgang MUSS PhD EM-Lab Univ. Inst. Pathology, SALK-LKH(Gen. Hospital) & PMU (Private Paracelsus Medical University) Salzburg SALZBURG - AUSTRIA
Von: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] Gesendet: Freitag, 31. Jänner 2014 20:11 An: Muß Wolfgang Betreff: [Microscopy] Re(Fwd): Unity Asset Management Service? [by companies, service contracts costs and trying to find cost reductions] // REMI-Service: Follow up on Consolidated Service Contract inquiry
[Microscopy] !! Re: Unity Asset Management Service? [by companies, service contracts costs and trying to find cost reductions] !! // Follow up on Consolidated Service Contract inquiry
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Hello everyone who are concerned with similar problem of pressure to consider a consolidated service contract.
There was a post by Richard Edelmann on Unity Asset Management Service. We are being urged to consider another service called Remi (state of Arkansas has a contract with them, I've been told):
https://www.theremigroup.com/default.aspx
Our main instruments are from JEOL (TEM/SEM), Bruker (XRD and AFM), Horiba (Raman, Photoluminescence, Elipsometry), Thermo Fisher (XPS), etc. Does anybody have experience with Remi service (especially with cases involving other vendors such as JEOL, Bruker-AXS, or Thermo Fisher)?
I have contacted Richard and have received reply from him already on Unity (thank you very much Richard for very quick reply). Any information on Remi would help us since we have no idea about them.
Thank you very much.
Fumiya
Fumiya Watanabe, Ph.D. Director of Instrumentation Center for Integrative Nanotechnology Sciences ETAS 153E University of Arkansas at Little Rock 2801 S. University Ave. Little Rock, AR 72204 (501) 683-7479 (Office)
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==============================Original Headers============================== 29, 40 -- From W.Muss-at-salk.at Mon Feb 3 09:34:30 2014 29, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 29, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s13FYTZV001453 29, 40 -- for {microscopy-at-microscopy.com} ; Mon, 3 Feb 2014 09:34:29 -0600 29, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 29, 40 -- by hermes.salk.at (Postfix) with ESMTP id 1A707646B2D 29, 40 -- for {microscopy-at-microscopy.com} ; Mon, 3 Feb 2014 16:34:28 +0100 (CET) 29, 40 -- X-Scanned-By: SALK-Content-Filter 29, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 29, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 29, 40 -- with ESMTP id 34aRAcCrjRRD for {microscopy-at-microscopy.com} ; 29, 40 -- Mon, 3 Feb 2014 16:34:27 +0100 (CET) 29, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 29, 40 -- by hermes.salk.at (Postfix) with ESMTP id CF9EE646B40 29, 40 -- for {microscopy-at-microscopy.com} ; Mon, 3 Feb 2014 16:34:27 +0100 (CET) 29, 40 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 29, 40 -- by N1EX198.lks.local with ESMTP; 03 Feb 2014 16:39:41 +0100 29, 40 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 29, 40 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 29, 40 -- 14.02.0387.000; Mon, 3 Feb 2014 16:34:27 +0100 29, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 29, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 29, 40 -- Subject: [Microscopy] Re(Fwd): Unity Asset Management Service? // 29, 40 -- REMI-Service: Follow up on Consolidated Service Contract inquiry 29, 40 -- Thread-Topic: [Microscopy] Re(Fwd): Unity Asset Management Service? // 29, 40 -- REMI-Service: Follow up on Consolidated Service Contract inquiry 29, 40 -- Thread-Index: AQHPIPVreSPfIxDLmEK+2UCpUnWrsw== 29, 40 -- Date: Mon, 3 Feb 2014 15:34:26 +0000 29, 40 -- Message-ID: {3D22E6EE65F39E448DCE4162815376711C57BF30-at-N2DT131.lksdom21.lks.local} 29, 40 -- References: {201401311910.s0VJAYg5006429-at-ns.microscopy.com} 29, 40 -- In-Reply-To: {201401311910.s0VJAYg5006429-at-ns.microscopy.com} 29, 40 -- Accept-Language: de-DE, en-US 29, 40 -- Content-Language: de-DE 29, 40 -- X-MS-Has-Attach: 29, 40 -- X-MS-TNEF-Correlator: 29, 40 -- x-originating-ip: [192.168.42.2] 29, 40 -- Content-Type: text/plain; charset="iso-8859-1" 29, 40 -- MIME-Version: 1.0 29, 40 -- Content-Transfer-Encoding: 8bit 29, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s13FYTZV001453 ==============================End of - Headers==============================
From boakkeau-at-wegl.net Mon Feb 3 09:59:44 2014 Return-Path: {boakkeau-at-wegl.net} Received: from mail3.wegl.net ([178.126.142.44]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s13FxfSr020679 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 3 Feb 2014 09:59:42 -0600 Message-Id: {201402031559.s13FxfSr020679-at-ns.microscopy.com}
Pete,
Films on grids do tend to break when old. I used some 75 mesh grids that were 5 years old recently and they were still good but I'd not try to use single holed ones that were that old unless I really needed to. With older 200 mesh grids one can usually get some areas that do not pop but use newer ones if you can afford them or make them yourself. Perhaps your drying step is a bit harsh. The word "blot" is what caught my eye. It is hard to judge unless it could be observed.
I was taught to do my negative staining differently than how you describe. I put a sample onto a recently glow discharged grid held in forcepts, let it sit for 30 to 60 seconds, gently drop 5-6 drops of 1%UA from a pipet onto the surface of the grid held perpendicular to the grid (grid parallel to the table top over a vessel to catch the UA). After the last drop of UA is on the grid I touch a point of filter paper to the junction of the forcepts where the grid is being held to slowly wick the UA off. The surface of the grid will still be wet. There is usually a small amount of UA between the forcept tips so I "push" the grid out of the forcepts with the same piece of filter paper using another portion that is not already wet, onto some lens tissue to continue to dry completely.
There are many different techniques in doing negative staining. Once you find one that works for you that is the one you should stick with.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Pat
Patricia Stranen Connelly NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491
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Email: pete.finger-at-jax.org Name: Pete Finger
Organization: The Jackson Laboratory
Title-Subject: [Filtered] Negative Staining Problem
Message: I?ve been doing some negative staining and am experiencing some problems with the Formvar film falling apart on the grid. I?ve been using 200 mesh Copper Formvar/carbon coated grids which are a couple of years old ? does anyone know if these grids have a certain shelf life? My technique has been to put a drop of the protein (in HEPES buffer) on the grid, blot dry, add a drop of uranyl acetate, blot dry and then let the grid air dry. When I view the grid on the TEM, pretty much all the Formvar is gone. I haven?t done a lot of negative staining in the past so any comments/suggestions would be greatly appreciated. Thanks much.
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==============================Original Headers============================== 21, 29 -- From connellyps-at-nhlbi.nih.gov Mon Feb 3 11:38:29 2014 21, 29 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s13HcQuu022068 21, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Feb 2014 11:38:28 -0600 21, 29 -- X-IronPortListener: Outbound_SMTP 21, 29 -- X-IronPort-Anti-Spam-Filtered: true 21, 29 -- X-IronPort-Anti-Spam-Result: AiUFABHT71KcKEeo/2dsb2JhbABWA4MMOFeDAbsIGHQWdIIlAQIEEiIaPQEVCgUICgEVBB0SAR0KBBMZBAQBh2MNkAmMYIR0ihEOoUSBII0HEQFdCwEBglOBUgSLU4MRiUaBMoU+izGDLYFqBxci 21, 29 -- Received: from unknown (HELO CASHTV04.nih.gov) ([156.40.71.168]) 21, 29 -- by nihxway2out.hub.nih.gov with ESMTP/TLS/AES128-SHA; 03 Feb 2014 12:38:25 -0500 21, 29 -- Received: from MLBXV14.nih.gov ([169.254.4.96]) by CASHTV04.nih.gov 21, 29 -- ([156.40.71.168]) with mapi id 14.03.0174.001; Mon, 3 Feb 2014 12:38:25 -0500 21, 29 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 21, 29 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 21, 29 -- Subject: Negative Staining Problem 21, 29 -- Thread-Topic: Negative Staining Problem 21, 29 -- Thread-Index: AQHPIQa9M4ubQaW/WkOnMozQy1n7dg== 21, 29 -- Date: Mon, 3 Feb 2014 17:38:25 +0000 21, 29 -- Message-ID: {CF153E40.12FD%connellyps-at-mail.nih.gov} 21, 29 -- Accept-Language: en-US 21, 29 -- Content-Language: en-US 21, 29 -- X-MS-Has-Attach: 21, 29 -- X-MS-TNEF-Correlator: 21, 29 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 21, 29 -- x-originating-ip: [156.40.71.189] 21, 29 -- Content-Type: text/plain; charset="big5" 21, 29 -- Content-ID: {6C33295EF427DB459068692462DC21D1-at-mail.nih.gov} 21, 29 -- MIME-Version: 1.0 21, 29 -- Content-Transfer-Encoding: 8bit 21, 29 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s13HcQuu022068 ==============================End of - Headers==============================
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Email: tjzhang2000-at-yahoo.com Name: Tim Zhang
Organization: GA Tech
Title-Subject: [Filtered] 200KV used TEM wanted
Message: Dear All,
We are looking for am used TEM, 200-300KV not FEG gun. If you know any info about it please let me know.
Thank you very much for your time and help.
Tim
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Email: 12hy1-at-queensu.ca Name: Hongbing Yu
Organization: Queens' University, Canada
Title-Subject: [Filtered] EDS can't detect tin
Message: We are trying to use FEI TECNAI ORISIS TEM characterize the element distribution of a Zirconium alloy with 3.5 atom% Sn. However we can't detect tin anywhere with EDS in STEM Mode with beam spot 9. The EDS system works very well for a ODS steel. We have tried our best to figure out this problem, but we failed. Any comment and suggestion would be appreciated.
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Email: slc6-at-lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh Microscopy School 2014
Message: There is still time to register for the 2014 Lehigh Microscopy School which will be held June 8-14, 2014. ALL courses will be offered in the same week which means that ALL lecturers and ALL instrument suppliers will be together for what promises to be a phenomenal week. This year will be the 44th anniversary for the Lehigh Microscopy School! Course offerings include: Scanning Electron Microscopy and X-ray Microanalysis • Introduction to SEM and EDS for the New Operator • Focused Ion Beam Instrumentation and Applications • Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD Data • Quantitative X-ray Microanalysis: Problem Solving Using EDS and WDS Techniques • Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register by April 15 for an Early Bird Discount! Contact Sharon Coe (sharon.coe-at-lehigh.edu or 610-758-5133). www.lehigh.edu/microscopy
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This may sound like a silly suggestion but if you haven't already, I would try the following things:
1) Use an enormous beam in TEM mode to look around on micron-scale and look for Sn in EDS all over any thin area. You may just be looking at an unusual part of the specimen where the Sn is gone and not know it.
2) If you can do EELS, check for Sn there - should be an M edge about 500eV I think (look in EELS Atlas, I am being vague). If you cannot see Sn anywhere by EELS or EDS then the problem is not the EDS detector, the problem is that your specimen doesn't have Sn in it, and you now have to figure out why. (Preferential removal of Sn during sample prep? Something happened when making the material originally?)
Good luck!
Yours
Jo Sharp
On 4 February 2014 21:16, {zaluzec-at-microscopy.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: 12hy1-at-queensu.ca () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: 12hy1-at-queensu.ca } Name: Hongbing Yu } } Organization: Queens' University, Canada } } Title-Subject: [Filtered] EDS can't detect tin } } Message: We are trying to use FEI TECNAI ORISIS TEM characterize the } element distribution of a Zirconium alloy with 3.5 atom% Sn. However we } can't detect tin anywhere with EDS in STEM Mode with beam spot 9. The } EDS system works very well for a ODS steel. We have tried our best to } figure out this problem, but we failed. Any comment and suggestion would } be appreciated. } } Login Host: 130.15.116.215 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } -- } =============================================== } } Do not reply to this messages it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================= } } ==============================Original Headers============================== } 14, 39 -- From nestor.zaluzec-at-gmail.com Tue Feb 4 15:01:57 2014 } 14, 39 -- Received: from mail-pb0-f52.google.com (mail-pb0-f52.google.com [209.85.160.52]) } 14, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s14L1uOP006323 } 14, 39 -- for {microscopy-at-microscopy.com} ; Tue, 4 Feb 2014 15:01:57 -0600 } 14, 39 -- Received: by mail-pb0-f52.google.com with SMTP id jt11so8922418pbb.25 } 14, 39 -- for {microscopy-at-microscopy.com} ; Tue, 04 Feb 2014 13:01:56 -0800 (PST) } 14, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 39 -- d=gmail.com; s=20120113; } 14, 39 -- h=sender:message-id:date:from:reply-to:user-agent:mime-version:to } 14, 39 -- :subject:references:in-reply-to:content-type } 14, 39 -- :content-transfer-encoding; } 14, 39 -- bh=klHeYixvGJoedxJSpxTWqT+OgUGT4cK30LG0Velz21c=; } 14, 39 -- b=VHelNtSKe4o6QEI7zC8SiSpg+d6O7gCFA5BJI2X2uU7p7TMZjShyCgixgeYbGPeETV } 14, 39 -- jUOtDTuX5RrTZNezNYlaJvmTlKbVQGW8J2FmJonM/kfjPDqyE8sEoMSS+gl9Fc1TDjVY } 14, 39 -- LRrrK+BDobBp7uUAHQoE/HHOMlH6KGCWOwLyjD/gE9wlAkP8Fx6CZdiQYjWdR74DG2iL } 14, 39 -- wmzcxyzi4pxXw1d0S0o4rhltjFldrMeltxpLgfi9C0OOwewSfwn2Cp6X/MzOg+l5PRTf } 14, 39 -- Wr2wgfmYuxeVQNVYsn8ZWdErsfLXRFTKHJ/PdlNwIjNe/lW8YbMaahDkeb0ctNZAi60u } 14, 39 -- UPvw== } 14, 39 -- X-Received: by 10.66.154.169 with SMTP id vp9mr45713120pab.39.1391547716282; } 14, 39 -- Tue, 04 Feb 2014 13:01:56 -0800 (PST) } 14, 39 -- Received: from NestorPwrBk.local ([210.9.11.226]) } 14, 39 -- by mx.google.com with ESMTPSA id nu10sm69172882pbb.16.2014.02.04.13.01.54 } 14, 39 -- for {microscopy-at-microscopy.com} } 14, 39 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); } 14, 39 -- Tue, 04 Feb 2014 13:01:55 -0800 (PST) } 14, 39 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} } 14, 39 -- Message-ID: {52F15540.5030100-at-microscopy.com} } 14, 39 -- Date: Wed, 05 Feb 2014 07:31:52 +1030 } 14, 39 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} } 14, 39 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 14, 39 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 } 14, 39 -- MIME-Version: 1.0 } 14, 39 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 39 -- Subject: viaWWW:EDS can't detect tin } 14, 39 -- References: {201402041501.s14F1HJL030540-at-ns.microscopy.com} } 14, 39 -- In-Reply-To: {201402041501.s14F1HJL030540-at-ns.microscopy.com} } 14, 39 -- X-Forwarded-Message-Id: {201402041501.s14F1HJL030540-at-ns.microscopy.com} } 14, 39 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 14, 39 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 39 -- From j.sharp-at-sheffield.ac.uk Wed Feb 5 06:42:39 2014 8, 39 -- Received: from mailhost1.shef.ac.uk (mailhost1.shef.ac.uk [143.167.251.162]) 8, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s15Cgd18013449 8, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Feb 2014 06:42:39 -0600 8, 39 -- Received: from mail-yk0-f171.google.com ([209.85.160.171]) 8, 39 -- by mailhost1.shef.ac.uk with esmtps (TLSv1:RC4-SHA:128) 8, 39 -- (Exim 4.77) 8, 39 -- (envelope-from {j.sharp-at-sheffield.ac.uk} ) 8, 39 -- id 1WB1oL-00032o-VF 8, 39 -- for Microscopy-at-microscopy.com; Wed, 05 Feb 2014 12:42:38 +0000 8, 39 -- Received: by mail-yk0-f171.google.com with SMTP id 142so784856ykq.2 8, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 05 Feb 2014 04:42:36 -0800 (PST) 8, 39 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 39 -- d=1e100.net; s=20130820; 8, 39 -- h=x-gm-message-state:mime-version:in-reply-to:references:date 8, 39 -- :message-id:subject:from:to:content-type; 8, 39 -- bh=Q3Kfa1KJ8S4ket2YYdu0cCGxK5z3tlo6rSpK1gm7GT0=; 8, 39 -- b=PxtBfmdNr/g9t4OCxISV/xUW3LfyXUceoH5ZxbCmUyyY8WI6wntEooyamUasxIIJjE 8, 39 -- c8w3zXsSWiBDEtmXj7Xgum7YwCI8MIS/wNDIkw5qD6fmoIy9WT9Bp8hoFsYHzpDbCktO 8, 39 -- IGK8/1fTCw/xMfwtDQE6sNVsnU07OvjzQNM5LC/d7jCb6bygUGuWVPVBaRShZ4koFmFn 8, 39 -- 8foKD4w5TLhkD/v+EWTTwfqk99qXz//ull2COcHQYpf0JyiWrivNnmAyppOGL89yYs+i 8, 39 -- U8liwh64CGGbG+nG901eLwbiN+AIrQGQVID5QiIot86Pwtf66AUg0D/nL1X2I+lRv9C2 8, 39 -- Fs+w== 8, 39 -- X-Gm-Message-State: ALoCoQkVAsqF2fwr1rkK/vq33Pw2mNgPV/XlFE8RcPkO1xKZQbx0fztWe3LYtfejRN0mjXnZpW76Yl85PSffxB/Si4j60fxyMM6JxcjnlC0nA7tNGSBOmKqRsI/G5iYOEGngsVu7F12eqIrs4qc7CS+etaCfKfXFZxR6WsV3Y+6RumbLFGXXKLs= 8, 39 -- X-Received: by 10.236.38.74 with SMTP id z50mr154390yha.134.1391604156934; 8, 39 -- Wed, 05 Feb 2014 04:42:36 -0800 (PST) 8, 39 -- MIME-Version: 1.0 8, 39 -- X-Received: by 10.236.38.74 with SMTP id z50mr154380yha.134.1391604156804; 8, 39 -- Wed, 05 Feb 2014 04:42:36 -0800 (PST) 8, 39 -- Received: by 10.170.63.197 with HTTP; Wed, 5 Feb 2014 04:42:36 -0800 (PST) 8, 39 -- In-Reply-To: {201402042116.s14LGSVL032283-at-ns.microscopy.com} 8, 39 -- References: {201402042116.s14LGSVL032283-at-ns.microscopy.com} 8, 39 -- Date: Wed, 5 Feb 2014 12:42:36 +0000 8, 39 -- Message-ID: {CAG8d=kXyexSXXmA3JFNt8mTUU_VoKn=gPycaMMOQ36q1VCsRWg-at-mail.gmail.com} 8, 39 -- Subject: Re: [Microscopy] viaWWW:EDS can't detect tin 8, 39 -- From: Joanne Sharp {j.sharp-at-sheffield.ac.uk} 8, 39 -- To: 12hy1-at-queensu.ca, Microscopy-at-microscopy.com 8, 39 -- Content-Type: text/plain; charset=ISO-8859-1 8, 39 -- X-S0phie-Scan: No ==============================End of - Headers==============================
March 3-7, 2014 a course in polarized light microscopy will be offered at Hooke College of Applied Sciences. Further information about this hands-on course can be found at the link below:
Best regards- __________________________________________________ Chris Gorman Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax) www.hookecollege.com
==============================Original Headers============================== 7, 25 -- From CGorman-at-hookecollege.com Wed Feb 5 11:58:21 2014 7, 25 -- Received: from mail.mccrone.com (mail.mccrone.com [12.54.22.114]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s15HwLCN003704 7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 5 Feb 2014 11:58:21 -0600 7, 25 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by 7, 25 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Wed, 5 Feb 2014 11:58:19 7, 25 -- -0600 7, 25 -- From: Christine Gorman {CGorman-at-hookecollege.com} 7, 25 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 25 -- Date: Wed, 5 Feb 2014 11:58:18 -0600 7, 25 -- Subject: PLM Short Course Announcement: March 3-7, 2014 7, 25 -- Thread-Topic: PLM Short Course Announcement: March 3-7, 2014 7, 25 -- Thread-Index: Ac8im738S+31181IScWvZZxXDySEmQ== 7, 25 -- Message-ID: {874B1DB532886444A60A015EE363493F9D84EB29E6-at-mccronemsg07.tmg.mccrone.com} 7, 25 -- Accept-Language: en-US 7, 25 -- Content-Language: en-US 7, 25 -- X-MS-Has-Attach: 7, 25 -- X-MS-TNEF-Correlator: 7, 25 -- x-ninja-pim: Scanned by Ninja 7, 25 -- x-ninja-attachmentfiltering: (no action) 7, 25 -- acceptlanguage: en-US 7, 25 -- Content-Type: text/plain; charset="us-ascii" 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s15HwLCN003704 ==============================End of - Headers==============================
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Message: MSNO Winter MEETING will be on Wednesday, February 26th, 2014, 4:00 – 8:30 p.m. Dittrick Medical History Center Allen Memorial Medical Library 11000 Euclid Ave. Cleveland, OH 44106-1714
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anybody out there to help me with a service manual and a parts list for the Reichert Ultracut E ultramicrotome? All I have is the user manual.
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
I was just sent some material on a new microscope from Seron and it occurred to me I have not really heard much on the list about this brand. Has anyone tried one or have one even? How do they fair to other entry level micorscopes. Comments on or off list would be appricated.
Thanks John in the UK (Bath Uni)
==============================Original Headers============================== 3, 26 -- From john.mitchels-at-gmail.com Thu Feb 6 07:47:47 2014 3, 26 -- Received: from mail-vb0-f46.google.com (mail-vb0-f46.google.com [209.85.212.46]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s16DlkqV029411 3, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Feb 2014 07:47:46 -0600 3, 26 -- Received: by mail-vb0-f46.google.com with SMTP id o19so1405181vbm.19 3, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 06 Feb 2014 05:47:46 -0800 (PST) 3, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=20120113; 3, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 26 -- bh=WgJjxQNNeV1lxO2rTvgtlydWlgDE+GmL4qAuWaEmUSQ=; 3, 26 -- b=ydBjVMSMrl6ozJn3iXeLDBns4C/0vu8W0ZP1iEw3FlI/J9md6F9tQlAx1t4ItMo+i2 3, 26 -- rcYWaCOi8hmE6ivDp7CasFzYkAdcZbkinkOU0vuaP80r0B5yB8bLtta7JyUTgv7a0s4v 3, 26 -- i4C913ans5M77zpaIFD/Kb1flvOb9j8izKJNIgcxPnP/U3rSBhOXjBJSmpQn6wS/l0UM 3, 26 -- QknMrRo6baTdUGugLCAAH6p8xJl3pRdBBCPP18UHCx4bEMD1MAO293mDi+qpf4cquSE+ 3, 26 -- uLQ9NBggFVC7fE0WqH8krU8NK2G/0IvjN3X/ug344HpM+uhgsvAid0NsRSwXFrhUqfT8 3, 26 -- L3SQ== 3, 26 -- MIME-Version: 1.0 3, 26 -- X-Received: by 10.52.107.35 with SMTP id gz3mr4917851vdb.8.1391694466192; Thu, 3, 26 -- 06 Feb 2014 05:47:46 -0800 (PST) 3, 26 -- Received: by 10.220.165.68 with HTTP; Thu, 6 Feb 2014 05:47:46 -0800 (PST) 3, 26 -- Date: Thu, 6 Feb 2014 13:47:46 +0000 3, 26 -- Message-ID: {CAHX7yTQnjfh+sdvbbLhgnGUS2w74+H21YjM5NWkesVbPJXh87Q-at-mail.gmail.com} 3, 26 -- Subject: Seron SEMs any views? 3, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 3, 26 -- To: Microscopy-at-microscopy.com 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Dear All, thanks, I finally got a PDF of the service manual.
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 06.02.14 14:28, schrieb Muß Wolfgang: } bradford.ross-at-botany.ubc.ca {mailto:bradford.ross-at-botany.ubc.ca} } } Stefan: } } ich bin ja schon sehr froh, wenn ich als „Notnagel“ wenigstens da und dort einmal was „tun“ kann für die Community....Für Dich } allemal von Herzen! } } Habe noch was gefunden (originales pdf hatte 13 MB, daher habe ich es nochmals als pdf „gedruckt“ und damit auf ca. 7MB } komprimiert...das hat etwas gedauert...) } } Die Dokumentation hatte ich dankenswerterweise von bradford.ross-at-botany.ubc.ca {mailto:bradford.ross-at-botany.ubc.ca} im Nov. 2013 } erhalten. } } Ebenfalls „sonnige“, und v. a. {föhnige} Grüße aus Salzburg (dzt. wie im späten April: knallblauer Himmel bei ca. 15Grad C! } } Wolfgang } } *Von:*Stefan Diller [mailto:stefan.diller-at-t-online.de] } *Gesendet:* Donnerstag, 06. Februar 2014 14:21 } *An:* Muß Wolfgang } *Betreff:* Re: persönl. AW(2): [Microscopy] Reichert Ultracut E service manual } } Lieber Wolfgang, } wie immer der Retter in der Not ;-) } } Herzliche Grüße in den Süden, } Stefan } } Am 06.02.2014 14:18, schrieb Muß Wolfgang: } } Lieber Stefan, } } das ist alles, was ich in meinen files gefunden habe.... } } mhG } } und beste Wünsche beim Basteln... } } Wolfgang } } *Von:*stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} [mailto:stefan.diller-at-t-online.de] } *Gesendet:* Donnerstag, 06. Februar 2014 11:02 } *An:* Muß Wolfgang } *Betreff:* [Microscopy] Reichert Ultracut E service manual } } --------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } Dear All, } } anybody out there to help me with a service manual and a parts list for } } the Reichert Ultracut E ultramicrotome? } } All I have is the user manual. } } Thanks, } } Stefan } } -- } } ----------------------------------------------------- } } Stefan Diller - Scientific Photography } } Arndtstrasse 22 } } D - 97072 Wuerzburg Germany } } ++49-931-7848700 Phone } } ++49-931-7848701 Fax } } ++49-175-7177051 Mobile } } Websites: } } www.nanoflight.info {http://www.nanoflight.info} } } www.electronmicroscopy.info {http://www.electronmicroscopy.info} } } www.elektronenmikroskopie.info {http://www.elektronenmikroskopie.info} } } www.assisi.de {http://www.assisi.de} } } www.zwillingsprojekt.de {http://www.zwillingsprojekt.de} } } www.stefan-diller.com {http://www.stefan-diller.com} } } Anfahrt: http://Mail.map24.com/Stefan.Diller } } ----------------------------------------------------- } } ==============================Original Headers============================== } } 9, 20 -- From stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} Thu Feb 6 03:57:17 2014 } } 9, 20 -- Received: from mailout05.t-online.de (mailout05.t-online.de [194.25.134.82]) } } 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s169vG4m007656 } } 9, 20 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 6 Feb 2014 03:57:16 -0600 } } 9, 20 -- Received: from fwd27.aul.t-online.de (fwd27.aul.t-online.de ) } } 9, 20 -- by mailout05.t-online.de with smtp } } 9, 20 -- id 1WBLhp-0003UD-Oc; Thu, 06 Feb 2014 10:57:14 +0100 } } 9, 20 -- Received: from [192.168.2.202] (S90VZgZOgh7NeC2laO0ho+P-GiMlg8xpksETwKZJ07y+8vUsNdUHVcgzJrB2Z5IwK2-at-[91.58.180.49 } {mailto:S90VZgZOgh7NeC2laO0ho+P-GiMlg8xpksETwKZJ07y+8vUsNdUHVcgzJrB2Z5IwK2-at-[91.58.180.49} ]) by fwd27.t-online.de } } 9, 20 -- with esmtp id 1WBLhi-1sCXNA0; Thu, 6 Feb 2014 10:57:06 +0100 } } 9, 20 -- Message-ID: {52F35C74.2020707-at-t-online.de {mailto:52F35C74.2020707-at-t-online.de} } } } 9, 20 -- Date: Thu, 06 Feb 2014 10:57:08 +0100 } } 9, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} } } } 9, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 } } 9, 20 -- MIME-Version: 1.0 } } 9, 20 -- To: microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 9, 20 -- Subject: Reichert Ultracut E service manual } } 9, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } } 9, 20 -- Content-Transfer-Encoding: 7bit } } 9, 20 -- X-ID: S90VZgZOgh7NeC2laO0ho+P-GiMlg8xpksETwKZJ07y+8vUsNdUHVcgzJrB2Z5IwK2 } } 9, 20 -- X-TOI-MSGID: bd40bafa-19ef-4dcc-b708-8ce2056c24bc } } ==============================End of - Headers============================== } } } } -- } } } } } } } } ----------------------------------------------------- } } Stefan Diller - Scientific Photography } } Arndtstrasse 22 } } D - 97072 Wuerzburg Germany } } ++49-931-7848700 Phone } } ++49-931-7848701 Fax } } ++49-175-7177051 Mobile } } } } Websites: } } www.nanoflight.info {http://www.nanoflight.info} } } www.electronmicroscopy.info {http://www.electronmicroscopy.info} } } www.elektronenmikroskopie.info {http://www.elektronenmikroskopie.info} } } www.assisi.de {http://www.assisi.de} } } www.zwillingsprojekt.de {http://www.zwillingsprojekt.de} } } www.stefan-diller.com {http://www.stefan-diller.com} } } Anfahrt:http://Mail.map24.com/Stefan.Diller } } ----------------------------------------------------- }
==============================Original Headers============================== 9, 23 -- From stefan.diller-at-t-online.de Thu Feb 6 08:43:56 2014 9, 23 -- Received: from mailout07.t-online.de (mailout07.t-online.de [194.25.134.83]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s16Eht6u016458 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2014 08:43:55 -0600 9, 23 -- Received: from fwd30.aul.t-online.de (fwd30.aul.t-online.de ) 9, 23 -- by mailout07.t-online.de with smtp 9, 23 -- id 1WBQBF-00056Z-RM; Thu, 06 Feb 2014 15:43:53 +0100 9, 23 -- Received: from mac-pro.speedport.ip (VmpAcqZCrh1txE7m6SOz7MPAk7Om5JjbWiIS7qZ2+4SLtNlgHCNp8xu25vcJumeQMy-at-[84.174.121.68]) by fwd30.t-online.de 9, 23 -- with esmtp id 1WBQBF-0MAdUG0; Thu, 6 Feb 2014 15:43:53 +0100 9, 23 -- Message-ID: {52F39FA9.6030306-at-t-online.de} 9, 23 -- Date: Thu, 06 Feb 2014 15:43:53 +0100 9, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 9, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 9, 23 -- MIME-Version: 1.0 9, 23 -- To: microscopy-at-microscopy.com 9, 23 -- Subject: Re: [Microscopy] Reichert Ultracut E service manual 9, 23 -- References: {201402061002.s16A25H5012082-at-ns.microscopy.com} {3D22E6EE65F39E448DCE4162815376711C58A178-at-N2DT131.lksdom21.lks.local} {52F38C47.8030604-at-t-online.de} {3D22E6EE65F39E448DCE4162815376711C58A77C-at-N2DT131.lksdom21.lks.local} 9, 23 -- In-Reply-To: {3D22E6EE65F39E448DCE4162815376711C58A77C-at-N2DT131.lksdom21.lks.local} 9, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 9, 23 -- X-ID: VmpAcqZCrh1txE7m6SOz7MPAk7Om5JjbWiIS7qZ2+4SLtNlgHCNp8xu25vcJumeQMy 9, 23 -- X-TOI-MSGID: 99d1ef5d-1298-431a-9657-4f288b4ebe1e 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s16Eht6u016458 ==============================End of - Headers==============================
I originally posted this as "free SEM" but Nestor's spam filter threw me under the bus...
A friend of mine passed this ad on to me. I have no connection to the people giving this away, just thought someone out there might be interested. I'm not familiar with the make/model. Anybody? It does appear to be "vintage"...
Hello Microscopists, Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great.
Thanks for your thoughts and answers in advance Alejandro Hinojos
==============================Original Headers============================== 6, 29 -- From ahinojos2-at-miners.utep.edu Thu Feb 6 20:17:12 2014 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172HCnn004797 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Feb 2014 20:17:12 -0600 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com (10.255.210.155) by 6, 29 -- BLUPR05MB006.namprd05.prod.outlook.com (10.255.210.142) with Microsoft SMTP 6, 29 -- Server (TLS) id 15.0.868.8; Fri, 7 Feb 2014 02:17:10 +0000 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.44]) by 6, 29 -- BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.156]) with mapi id 6, 29 -- 15.00.0868.013; Fri, 7 Feb 2014 02:17:10 +0000 6, 29 -- From: "Hinojos, Alejandro" {ahinojos2-at-miners.utep.edu} 6, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 6, 29 -- Subject: TEM Acquisition Help 6, 29 -- Thread-Topic: TEM Acquisition Help 6, 29 -- Thread-Index: AQHPI6qIDXb0k8Img0O3WLndhK0Dmg== 6, 29 -- Date: Fri, 7 Feb 2014 02:17:10 +0000 6, 29 -- Message-ID: {1e44fc83cbc14812ac71282723f64e06-at-BLUPR05MB008.namprd05.prod.outlook.com} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- x-originating-ip: [129.108.37.93] 6, 29 -- x-forefront-prvs: 011579F31F 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(199002)(189002)(74876001)(53806001)(90146001)(76482001)(31966008)(74706001)(76796001)(56816005)(76176001)(75432001)(54316002)(92566001)(81816001)(85852003)(87936001)(63696002)(46102001)(76576001)(76786001)(77096001)(51856001)(54356001)(66066001)(65816001)(74502001)(81686001)(74662001)(47446002)(47976001)(2656002)(83322001)(56776001)(81342001)(94946001)(94316002)(87266001)(33646001)(81542001)(47736001)(83072002)(93516002)(74316001)(86362001)(74366001)(50986001)(93136001)(80022001)(69226001)(79102001)(4396001)(59766001)(80976001)(85306002)(49866001)(95416001)(77982001)(24736002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR05MB006;H:BLUPR05MB008.namprd05.prod.outlook.com;CLIP:129.108.37.93;FPR:3ADEF03E.9E339C09.87D2589.84EF8869.201A3;InfoNoRecordsA:0;MX:1;LANG:en; 6, 29 -- Content-Type: text/plain; charset="Windows-1252" 6, 29 -- MIME-Version: 1.0 6, 29 -- X-OriginatorOrg: miners.utep.edu 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172HCnn004797 ==============================End of - Headers==============================
It is a 5kV instrument. For TEM (or STEM), ask yourself what thickness you can look through with a 5kV beam.
Cheers, Henk
On 2/6/2014 9:19 PM, ahinojos2-at-miners.utep.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hello Microscopists, } Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great. } } } Thanks for your thoughts and answers in advance } Alejandro Hinojos } } } } } ==============================Original Headers============================== } 6, 29 -- From ahinojos2-at-miners.utep.edu Thu Feb 6 20:17:12 2014 } 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) } 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172HCnn004797 } 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Feb 2014 20:17:12 -0600 } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com (10.255.210.155) by } 6, 29 -- BLUPR05MB006.namprd05.prod.outlook.com (10.255.210.142) with Microsoft SMTP } 6, 29 -- Server (TLS) id 15.0.868.8; Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.44]) by } 6, 29 -- BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.156]) with mapi id } 6, 29 -- 15.00.0868.013; Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- From: "Hinojos, Alejandro" {ahinojos2-at-miners.utep.edu} } 6, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 6, 29 -- Subject: TEM Acquisition Help } 6, 29 -- Thread-Topic: TEM Acquisition Help } 6, 29 -- Thread-Index: AQHPI6qIDXb0k8Img0O3WLndhK0Dmg== } 6, 29 -- Date: Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- Message-ID: {1e44fc83cbc14812ac71282723f64e06-at-BLUPR05MB008.namprd05.prod.outlook.com} } 6, 29 -- Accept-Language: en-US } 6, 29 -- Content-Language: en-US } 6, 29 -- X-MS-Has-Attach: } 6, 29 -- X-MS-TNEF-Correlator: } 6, 29 -- x-originating-ip: [129.108.37.93] } 6, 29 -- x-forefront-prvs: 011579F31F } 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(199002)(189002)(74876001)(53806001)(90146001)(76482001)(31966008)(74706001)(76796001)(56816005)(76176001)(75432001)(54316002)(92566001)(81816001)(85852003)(87936001)(63696002)(46102001)(76576001)(76786001)(77096001)(51856001)(54356001)(66066001)(65816001)(74502001)(81686001)(74662001)(47446002)(47976001)(2656002)(83322001)(56776001)(81342001)(94946001)(94316002)(87266001)(33646001)(81542001)(47736001)(83072002)(93516002)(74316001)(86362001)(74366001)(50986001)(93136001)(80022001)(69226001)(79102001)(4396001)(59766001)(80976001)(85306002)(49866001)(95416001)(77982001)(24736002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR05MB006;H:BLUPR05MB008.namprd05.prod.outlook.com;CLIP:129.108.37.93;FPR:3ADEF03E.9E339C09.87D2589.84EF8869.201A3;InfoNoRecordsA:0;MX:1;LANG:en; } 6, 29 -- Content-Type: text/plain; charset="Windows-1252" } 6, 29 -- MIME-Version: 1.0 } 6, 29 -- X-OriginatorOrg: miners.utep.edu } 6, 29 -- Content-Transfer-Encoding: 8bit } 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172HCnn004797 } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 49 -- From colijn.1-at-osu.edu Thu Feb 6 20:27:29 2014 8, 49 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1ehsobe004.messaging.microsoft.com [216.32.181.184]) 8, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172RTQS017894 8, 49 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2014 20:27:29 -0600 8, 49 -- Received: from mail80-ch1-R.bigfish.com (10.43.68.251) by 8, 49 -- CH1EHSOBE008.bigfish.com (10.43.70.58) with Microsoft SMTP Server id 8, 49 -- 14.1.225.22; Fri, 7 Feb 2014 02:27:28 +0000 8, 49 -- Received: from mail80-ch1 (localhost [127.0.0.1]) by mail80-ch1-R.bigfish.com 8, 49 -- (Postfix) with ESMTP id 9CCA21404E4 for {microscopy-at-microscopy.com} ; Fri, 7 8, 49 -- Feb 2014 02:27:28 +0000 (UTC) 8, 49 -- X-Forefront-Antispam-Report: CIP:164.107.81.208;KIP:(null);UIP:(null);IPV:NLI;H:cio-krc-pf01;RD:none;EFVD:NLI 8, 49 -- X-SpamScore: 3 8, 49 -- X-BigFish: VPS3(zzbb2dI98dI9371Ic85ehe0eah1432Izz1f42h2148h1d77h208ch1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch1fc6hzd9hz1de098h17326ah8275bh1de097h186068hz2fh2a8h839h946hd25he5bhf0ah1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh162dh1631h1758h1765h18e1h190ch1946h19b4h19c3h19ceh1ad9h1b0ah224fh1d0ch1d2eh1d3fh1dfeh1dffh1f5fh1fe8h1ff5h209eh22d0h2336h2438h2461h2476h2487h24d7h2516h2545h1b1cn1b1bi1155h) 8, 49 -- Received-SPF: pass (mail80-ch1: domain of osu.edu designates 164.107.81.208 as permitted sender) client-ip=164.107.81.208; envelope-from=colijn.1-at-osu.edu; helo=cio-krc-pf01 ;cio-krc-pf01 ; 8, 49 -- Received: from mail80-ch1 (localhost.localdomain [127.0.0.1]) by mail80-ch1 8, 49 -- (MessageSwitch) id 1391740045621553_29835; Fri, 7 Feb 2014 02:27:25 +0000 8, 49 -- (UTC) 8, 49 -- Received: from CH1EHSMHS034.bigfish.com (snatpool1.int.messaging.microsoft.com 8, 49 -- [10.43.68.241]) by mail80-ch1.bigfish.com (Postfix) with ESMTP id 8, 49 -- 9227A2E007D; Fri, 7 Feb 2014 02:27:25 +0000 (UTC) 8, 49 -- Received: from cio-krc-pf01 (164.107.81.208) by CH1EHSMHS034.bigfish.com 8, 49 -- (10.43.70.34) with Microsoft SMTP Server (TLS) id 14.16.227.3; Fri, 7 Feb 8, 49 -- 2014 02:27:25 +0000 8, 49 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) (using TLSv1 8, 49 -- with cipher RC4-MD5 (128/128 bits)) (No client certificate requested) by 8, 49 -- cio-krc-pf01 (Postfix) with ESMTPS id A5FF3A005F; Thu, 6 Feb 2014 21:27:24 8, 49 -- -0500 (EST) 8, 49 -- Received: from [192.168.1.102] (75.118.135.65) by smtp.service.osu.edu 8, 49 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.174.1; Thu, 6 Feb 8, 49 -- 2014 21:27:24 -0500 8, 49 -- Message-ID: {52F4448A.8090904-at-osu.edu} 8, 49 -- Date: Thu, 6 Feb 2014 21:27:22 -0500 8, 49 -- From: Henk Colijn {colijn.1-at-osu.edu} 8, 49 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 8, 49 -- MIME-Version: 1.0 8, 49 -- To: {ahinojos2-at-miners.utep.edu} 8, 49 -- CC: {microscopy-at-microscopy.com} 8, 49 -- Subject: Re: [Microscopy] TEM Acquisition Help 8, 49 -- References: {201402070219.s172JFT5006464-at-ns.microscopy.com} 8, 49 -- In-Reply-To: {201402070219.s172JFT5006464-at-ns.microscopy.com} 8, 49 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 8, 49 -- X-Originating-IP: [75.118.135.65] 8, 49 -- X-CFilter-Loop: Forward 8, 49 -- X-FOPE-CRA-Verdict: 164.107.81.208$miners.utep.edu%0%1%osu.edu%False%False%0$ 8, 49 -- X-OriginatorOrg: osu.edu 8, 49 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 8, 49 -- X-FOPE-CONNECTOR: Id%0$Dn%MINERS.UTEP.EDU$RO%1$TLS%0$FQDN%$TlsDn% 8, 49 -- Content-Transfer-Encoding: 8bit 8, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172RTQS017894 ==============================End of - Headers==============================
A collegue was interested by this instrument last year and made some tests. His goal was to have an easy access to a TEM like instrument, to make fast controls in nanoparticules syntesis (organics with heavy metals), as the acess to our TEMs need some days of delay.
Hearing about the price of that instrument, I proposed him to test too a STEM device to be fitted on our FE-SEM. Comparing prices and performences (principally the versatility), the choice was easy. The STEM device for the SEM gives better images, the possibility to choose the HV up to 30 keV and a price 8x less expensive...
The real adavantage of this table-top TEM I could see is that it can be used easy by non-microcopists, like table-top SEMs, with on the other hand the limitations of the undestanding such people will have of what they do.
So if you soon have a (FE)SEM, it could be usefull to consider if the STEM device for SEM would not be a better solution. But I'm interested on what your tests will bring you as conclusions.
Hope it helps
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Le 07/02/2014 03:28, ahinojos2-at-miners.utep.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hello Microscopists, } Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great. } } } Thanks for your thoughts and answers in advance } Alejandro Hinojos } } } } } ==============================Original Headers============================== } 6, 29 -- From ahinojos2-at-miners.utep.edu Thu Feb 6 20:17:12 2014 } 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) } 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172HCnn004797 } 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Feb 2014 20:17:12 -0600 } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com (10.255.210.155) by } 6, 29 -- BLUPR05MB006.namprd05.prod.outlook.com (10.255.210.142) with Microsoft SMTP } 6, 29 -- Server (TLS) id 15.0.868.8; Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.44]) by } 6, 29 -- BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.156]) with mapi id } 6, 29 -- 15.00.0868.013; Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- From: "Hinojos, Alejandro" {ahinojos2-at-miners.utep.edu} } 6, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 6, 29 -- Subject: TEM Acquisition Help } 6, 29 -- Thread-Topic: TEM Acquisition Help } 6, 29 -- Thread-Index: AQHPI6qIDXb0k8Img0O3WLndhK0Dmg== } 6, 29 -- Date: Fri, 7 Feb 2014 02:17:10 +0000 } 6, 29 -- Message-ID: {1e44fc83cbc14812ac71282723f64e06-at-BLUPR05MB008.namprd05.prod.outlook.com} } 6, 29 -- Accept-Language: en-US } 6, 29 -- Content-Language: en-US } 6, 29 -- X-MS-Has-Attach: } 6, 29 -- X-MS-TNEF-Correlator: } 6, 29 -- x-originating-ip: [129.108.37.93] } 6, 29 -- x-forefront-prvs: 011579F31F } 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(199002)(189002)(74876001)(53806001)(90146001)(76482001)(31966008)(74706001)(76796001)(56816005)(76176001)(75432001)(54316002)(92566001)(81816001)(85852003)(87936001)(63696002)(46102001)(76576001)(76786001)(77096001)(51856001)(54356001)(66066001)(65816001)(74502001)(81686001)(74662001)(47446002)(47976001)(2656002)(83322001)(56776001)(81342001)(94946001)(94316002)(87266001)(33646001)(81542001)(47736001)(83072002)(93516002)(74316001)(86362001)(74366001)(50986001)(93136001)(80022001)(69226001)(79102001)(4396001)(59766001)(80976001)(85306002)(49866001)(95416001)(77982001)(24736002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR05MB006;H:BLUPR05MB008.namprd05.prod.outlook.com;CLIP:129.108.37.93;FPR:3ADEF03E.9E339C09.87D2589.84EF8869.201A3;InfoNoRecordsA:0;MX:1;LANG:en; } 6, 29 -- Content-Type: text/plain; charset="Windows-1252" } 6, 29 -- MIME-Version: 1.0 } 6, 29 -- X-OriginatorOrg: miners.utep.edu } 6, 29 -- Content-Transfer-Encoding: 8bit } 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172HCnn004797 } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 25 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Feb 7 02:01:34 2014 10, 25 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1781YdX013671 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2014 02:01:34 -0600 10, 25 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id 51B6224238B; 10, 25 -- Fri, 7 Feb 2014 09:01:52 +0100 (CET) 10, 25 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr 10, 25 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) 10, 25 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) 10, 25 -- with ESMTP id 458afHncdtNz; Fri, 7 Feb 2014 09:01:51 +0100 (CET) 10, 25 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id 3652524236C; 10, 25 -- Fri, 7 Feb 2014 09:01:51 +0100 (CET) 10, 25 -- Message-ID: {52F492D2.2060601-at-ipcms.u-strasbg.fr} 10, 25 -- Date: Fri, 07 Feb 2014 09:01:22 +0100 10, 25 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 25 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:17.0) Gecko/20130330 Thunderbird/17.0.5 10, 25 -- MIME-Version: 1.0 10, 25 -- To: ahinojos2-at-miners.utep.edu, microscopy-at-microscopy.com 10, 25 -- Subject: Re: [Microscopy] TEM Acquisition Help 10, 25 -- References: {201402070228.s172SkD0020695-at-ns.microscopy.com} 10, 25 -- In-Reply-To: {201402070228.s172SkD0020695-at-ns.microscopy.com} 10, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I have worked with a number of clients over the years, when we have used the SEM in a STEM mode. The advent of FEG systems dramatically changed the capabilities of the SEM to produce good quality STEM images, and the use of these instruments in x-ray analysis has been very fruitful. We have produced a paper that will be in the March "Microscopy Today", this summarises some of the work we have been doing. The use of thin films for x-ray analysis in the SEM, even without a STEM facility, is often a step forward that would have been more difficult to make if a dedicated TEM were used. There are many advantages to using a SEM for thin film x-ray analysis. The spectrum is less likely to be "contaminated" by signals from the instrument itself, and elemental mapping at high resolution is easily accomplished.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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realname - Alejandro Hinojos Email - ahinojos2-at-miners.utep.edu ORGANIZATION - University of Texas at El Paso EDUCATION - Undergraduate College LOCATION - El Paso SUBJECT_OF_QUESTION - TEM Shopping advice QUESTION - Hello Microscopists, Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM's? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great.
Thanks for your thoughts and answers in advance Alejandro Hinojos
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Organization: University of Texas at El Paso, Metalurgical & Materials Engineering Department
Title-Subject: [Filtered] EM Shopping
Message: Hello Microscopists, Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization. If anybody wouldn't mind sharing those experiences with us that would be great.
Thanks for your thoughts and answers in advance Alejandro Hinojos
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While I have a STEM/TEM, from time to time I use SEM/EDS for TEM grids with all advantages Steve mentioned. Just place several TEM grids with thin films on a carbon planchet, and go on! Remember: EDS resolution is determined mostly by thickness of specimen, not by instrument itself. So, EDS spatial resolution in SEM could be measured in nanometers (instead of microns) if thin specimens are used.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Friday, February 07, 2014 4:57 AM To: Dusevich, Vladimir
Hi
I have worked with a number of clients over the years, when we have used the SEM in a STEM mode. The advent of FEG systems dramatically changed the capabilities of the SEM to produce good quality STEM images, and the use of these instruments in x-ray analysis has been very fruitful. We have produced a paper that will be in the March "Microscopy Today", this summarises some of the work we have been doing. The use of thin films for x-ray analysis in the SEM, even without a STEM facility, is often a step forward that would have been more difficult to make if a dedicated TEM were used. There are many advantages to using a SEM for thin film x-ray analysis. The spectrum is less likely to be "contaminated" by signals from the instrument itself, and elemental mapping at high resolution is easily accomplished.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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Email: brian.miller-at-bruker-axs.com Name: brian miller
Organization: Bruker AXS
Title-Subject: [Filtered] EM Shopping
Message: in response to Alejandro's question and several responses I have been reading, I thought it would be good to clarify that the SEM can do nice analytical work in STEM mode and the new Bruker detector called the Flat Quad is a great contribution to this set up as it overcomes the issue of low signal from a TEM sample.
Although the LVEM5 from Delongh might be a very nice imaging system (I don't know but it does look very clever), going to their website and reading the brochure it is obvious that is in no way a system that can be used with any kind of EDS or EELs. So the analytical technique will be based entirely on interpreting the diffraction pattern if it is obtainable.
Than you, Brian Miller Bruker AXS (an OEM for xray detectors on SEM and TEM)
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I was just reading the article in the January 2014 Microscopy Today issue on standardizing color in digital images using the ChromaCal microscope slide and monitor calibration system. Interesting idea but I have a question. I know the authors Barbara Foster and Jerry Sedgewick participate on the listserver so I thought I would post it here for feedback from both them and others who might have used the system.
The website for Datacolor states "The CHROMACAL slide is not suitable for use with oil immersion objectives" but Figures 1 and 4 in the Microscopy Today article were made with oil immersion objectives.
The system looks to cost around $1000 so this won't be an impulse purchase for most of us. Anyone have experience or thoughts on this?
Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax)
==============================Original Headers============================== 7, 28 -- From PhillipsT-at-missouri.edu Mon Feb 10 14:08:10 2014 7, 28 -- Received: from um-nip3-missouri-out.um.umsystem.edu (um-nip3-missouri-out.um.umsystem.edu [198.209.49.163]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1AK8Auc027243 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 14:08:10 -0600 7, 28 -- X-IronPort-Anti-Spam-Filtered: true 7, 28 -- X-IronPort-Anti-Spam-Result: AhUFAC8x+VLPoJ7I/2dsb2JhbABWA4MMgQ+rdpBlgwaBFhZ0gicFgQcEASo6HCYBBBuHfZokqA6HDhcEjkhJgxOBFASJEaE6gy2CKg 7, 28 -- X-IPAS-Result: AhUFAC8x+VLPoJ7I/2dsb2JhbABWA4MMgQ+rdpBlgwaBFhZ0gicFgQcEASo6HCYBBBuHfZokqA6HDhcEjkhJgxOBFASJEaE6gy2CKg 7, 28 -- Received: from um-tcas3.um.umsystem.edu ([207.160.158.200]) 7, 28 -- by um-nip3-exch-relay.um.umsystem.edu with ESMTP; 10 Feb 2014 14:08:09 -0600 7, 28 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) by 7, 28 -- UM-TCAS3.um.umsystem.edu ([207.160.158.200]) with mapi id 14.03.0174.001; 7, 28 -- Mon, 10 Feb 2014 14:08:09 -0600 7, 28 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 7, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 7, 28 -- Subject: ChromaCal 7, 28 -- Thread-Topic: ChromaCal 7, 28 -- Thread-Index: Ac8mluadlg4QWVchQT27kPQM8IwZPgABD2yg 7, 28 -- Date: Mon, 10 Feb 2014 20:08:08 +0000 7, 28 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8EBE7F6-at-UM-MBX-T02.um.umsystem.edu} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- x-originating-ip: [128.206.81.115] 7, 28 -- Content-Type: text/plain; charset="iso-8859-1" 7, 28 -- MIME-Version: 1.0 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1AK8Auc027243 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Electron Microscopist Position Available - The Rockefeller University
Message: The Rockefeller University, a premier biomedical research institution, seeks a Research Support Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state of the art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a free substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Specialist will participate in all of the Electron Microscopy Resource Center's (EMRC) daily operations, including bench work in preparation for transmission and scanning electron microscopy and maintenance of the center. Will be responsible for all parts of sample preparation, including cutting ultrathin sections, maintenance and operation of EM and other equipment, training users, consulting with scientists on design of experiments, processing/analyzing data and interpretation of results, ordering and receiving supplies, managing chemical waste compliance, administrative support for office duties and center billing, and keeping up with the latest methodology via familiarity with the literature. Ph.D. in biology, cell biology, bioengineering or a related field required. Must have a minimum of 5 years of hands-on experience in electron microscopy and have strong communication skills, and the ability to work collaboratively on a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated. Expertise in cryo- 3D is a plus.
Our culture *inspiring, collaborative atmosphere *strong social and environmental consciousness *intellectually curious academic environment *14-acre campus setting in NYC
We offer a competitive salary, comprehensive benefits, and a beautiful work environment. To apply to this job, click the following URL, click on 'staff opportunities’ and enter keyword‘IRC11162’: http://www.rockefeller.edu/hr/career.php The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Kunihiro Uryu, Ph.D., Director of Electron Microscopy Resource Center
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Title-Subject: [Filtered] Parts to give away Zeiss DSM940
Message: Hi everyone, We have a few parts to give away for a SEM Zeiss DSM940(delivery at your charge)(Tungsten filament, stage, detector, etc). Contact me for more information!
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Title-Subject: [Filtered] Parts to give away Zeiss DSM940
Message: Hi everyone, We have a few parts to give away for a SEM Zeiss DSM940(delivery at your charge)(Tungsten filament, stage, detector, etc). Contact me for more information!
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X-from: kuryu-at-mail.rockefeller.edu () This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-mail.rockefeller.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Electron Microscopist Position Available - The Rockefeller University
Message: The Rockefeller University, a premier biomedical research institution, seeks a Research Support Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state of the art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a free substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Specialist will participate in all of the Electron Microscopy Resource Center's (EMRC) daily operations, including bench work in preparation for transmission and scanning electron microscopy and maintenance of the center. Will be responsible for all parts of sample preparation, including cutting ultrathin sections, maintenance and operation of EM and other equipment, training users, consulting with scientists on design of experiments, processing/analyzing data and interpretation of results, ordering and receiving supplies, managing chemical waste compliance, administrative support for office duties and center billing, and keeping up with the latest methodology via familiarity with the literature. Ph.D. in biology, cell biology, bioengineering or a related field required. Must have a minimum of 5 years of hands-on experience in electron microscopy and have strong communication skills, and the ability to work collaboratively on a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated. Expertise in cryo- 3D is a plus.
Our culture *inspiring, collaborative atmosphere *strong social and environmental consciousness *intellectually curious academic environment *14-acre campus setting in NYC
We offer a competitive salary, comprehensive benefits, and a beautiful work environment. To apply to this job, click the following URL, click on 'staff opportunities’ and enter keyword‘IRC11162’: http://www.rockefeller.edu/hr/career.php The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Kunihiro Uryu, Ph.D., Director of Electron Microscopy Resource Center
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==============================Original Headers============================== 18, 29 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb 11 00:52:51 2014 18, 29 -- Received: from znl.com ([206.69.208.20]) 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1B5Qa41022463 18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 23:26:36 -0600 18, 29 -- Received: from localhost (localhost [127.0.0.1]) 18, 29 -- by znl.com (Postfix) with ESMTP id 8CC0C6BA2CA 18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 23:26:36 -0600 (CST) 18, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 29 -- Received: from znl.com ([127.0.0.1]) 18, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 29 -- with ESMTP id Zv-iHGYu3ctM for {microscopy-at-microscopy.com} ; 18, 29 -- Mon, 10 Feb 2014 23:26:23 -0600 (CST) 18, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 29 -- by znl.com (Postfix) with ESMTPA id 2843C6BA2BD 18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 23:26:23 -0600 (CST) 18, 29 -- Message-ID: {52F9B47E.50708-at-microscopy.com} 18, 29 -- Date: Mon, 10 Feb 2014 23:26:22 -0600 18, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 18, 29 -- MIME-Version: 1.0 18, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 29 -- Subject: viaWWW:Electron Microscopist Position Available - The Rockefeller 18, 29 -- University 18, 29 -- References: {201402101542.s1AFgwWa021184-at-ns.microscopy.com} 18, 29 -- In-Reply-To: {201402101542.s1AFgwWa021184-at-ns.microscopy.com} 18, 29 -- X-Forwarded-Message-Id: {201402101542.s1AFgwWa021184-at-ns.microscopy.com} 18, 29 -- Content-Type: text/plain; charset=UTF-8; format=flowed 18, 29 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
True, the ChromaCal slide is not suitable for oil immersion objectives. The slide has no coverslip on the chip, and cleaning may damage the calibration matrix. Figures 1 and 4 were taken using an oil immersion lens for the sample, and a dry objective was used to image the ChromaCal slide. I found I could get equivalent results regardless of the objective used, as long as I kept the white balance the same, and set Koehler illumination.
Having run a light microscopy lab for 15 years, the issue of color inconsistency and inaccuracy led me to attempt making a color calibration slide on my own. I simply could not get the filters small enough for a microscope. Thus, my delight in working with a company that has accomplished it at this scale. To make things even better, I can do morphometry now using consistent color as a means to segment images.
Thanks a million for pointing out the disconnect in the article!
Jerry Sedgewick
On 2/10/2014 2:21 PM, PhillipsT-at-missouri.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was just reading the article in the January 2014 Microscopy Today issue on standardizing color in digital images using the ChromaCal microscope slide and monitor calibration system. Interesting idea but I have a question. I know the authors Barbara Foster and Jerry Sedgewick participate on the listserver so I thought I would post it here for feedback from both them and others who might have used the system. } } The website for Datacolor states "The CHROMACAL slide is not suitable for use with oil immersion objectives" but Figures 1 and 4 in the Microscopy Today article were made with oil immersion objectives. } } The system looks to cost around $1000 so this won't be an impulse purchase for most of us. Anyone have experience or thoughts on this? } } Tom } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } } } } ==============================Original Headers============================== } 7, 28 -- From PhillipsT-at-missouri.edu Mon Feb 10 14:08:10 2014 } 7, 28 -- Received: from um-nip3-missouri-out.um.umsystem.edu (um-nip3-missouri-out.um.umsystem.edu [198.209.49.163]) } 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1AK8Auc027243 } 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 10 Feb 2014 14:08:10 -0600 } 7, 28 -- X-IronPort-Anti-Spam-Filtered: true } 7, 28 -- X-IronPort-Anti-Spam-Result: AhUFAC8x+VLPoJ7I/2dsb2JhbABWA4MMgQ+rdpBlgwaBFhZ0gicFgQcEASo6HCYBBBuHfZokqA6HDhcEjkhJgxOBFASJEaE6gy2CKg } 7, 28 -- X-IPAS-Result: AhUFAC8x+VLPoJ7I/2dsb2JhbABWA4MMgQ+rdpBlgwaBFhZ0gicFgQcEASo6HCYBBBuHfZokqA6HDhcEjkhJgxOBFASJEaE6gy2CKg } 7, 28 -- Received: from um-tcas3.um.umsystem.edu ([207.160.158.200]) } 7, 28 -- by um-nip3-exch-relay.um.umsystem.edu with ESMTP; 10 Feb 2014 14:08:09 -0600 } 7, 28 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) by } 7, 28 -- UM-TCAS3.um.umsystem.edu ([207.160.158.200]) with mapi id 14.03.0174.001; } 7, 28 -- Mon, 10 Feb 2014 14:08:09 -0600 } 7, 28 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 7, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } 7, 28 -- Subject: ChromaCal } 7, 28 -- Thread-Topic: ChromaCal } 7, 28 -- Thread-Index: Ac8mluadlg4QWVchQT27kPQM8IwZPgABD2yg } 7, 28 -- Date: Mon, 10 Feb 2014 20:08:08 +0000 } 7, 28 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8EBE7F6-at-UM-MBX-T02.um.umsystem.edu} } 7, 28 -- Accept-Language: en-US } 7, 28 -- Content-Language: en-US } 7, 28 -- X-MS-Has-Attach: } 7, 28 -- X-MS-TNEF-Correlator: } 7, 28 -- x-originating-ip: [128.206.81.115] } 7, 28 -- Content-Type: text/plain; charset="iso-8859-1" } 7, 28 -- MIME-Version: 1.0 } 7, 28 -- Content-Transfer-Encoding: 8bit } 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1AK8Auc027243 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 37 -- From jerrysedgewick-at-gmail.com Tue Feb 11 08:11:59 2014 9, 37 -- Received: from mail-ig0-f170.google.com (mail-ig0-f170.google.com [209.85.213.170]) 9, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BEBx7H015254 9, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 08:11:59 -0600 9, 37 -- Received: by mail-ig0-f170.google.com with SMTP id m12so8843385iga.1 9, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 06:11:59 -0800 (PST) 9, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 37 -- d=gmail.com; s=20120113; 9, 37 -- h=message-id:date:from:user-agent:mime-version:to:subject:references 9, 37 -- :in-reply-to:content-type:content-transfer-encoding; 9, 37 -- bh=y1XepGT/ku7rbB67You8IUATjg7ZQm51LkKqei248qM=; 9, 37 -- b=K0g4FiTvCk09CcSQDySnYQXoz3o9FAxcY/xBxqA1GQv1K+Y3M4We53g48IZd2c4dgU 9, 37 -- U9Op11BkNUWfw0t48nTlACHB7OZjF6pkIqg8oGiwg37camOeayGTJiVAcc2etgW7aHMx 9, 37 -- tTgW7GfSw67fx25zKV8TTgRZ/AySIrOdEU6Z4IRbvYCRIkkAMvg+6JU370uekINBSRtT 9, 37 -- poO5Et2IHIqF+QQlx4HGhmXiG6mle4+/pOUK2ohS0kirVOtZttvS0xsAwSvTJnqD5Cpj 9, 37 -- B7Ay8U1+FRkFWKKO/xKtw263dXxEFTlZw/nLcGiZrcyOZcFMzlz7gKxHpdZgXFm1LeM2 9, 37 -- CqIg== 9, 37 -- X-Received: by 10.50.23.112 with SMTP id l16mr19766172igf.28.1392127919342; 9, 37 -- Tue, 11 Feb 2014 06:11:59 -0800 (PST) 9, 37 -- Received: from [127.0.0.1] (184-97-147-16.mpls.qwest.net. [184.97.147.16]) 9, 37 -- by mx.google.com with ESMTPSA id vn3sm15074124igb.0.2014.02.11.06.11.58 9, 37 -- for {Microscopy-at-microscopy.com} 9, 37 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 9, 37 -- Tue, 11 Feb 2014 06:11:58 -0800 (PST) 9, 37 -- Message-ID: {52FA2FAB.3030401-at-gmail.com} 9, 37 -- Date: Tue, 11 Feb 2014 08:11:55 -0600 9, 37 -- From: jerry sedgewick {jerrysedgewick-at-gmail.com} 9, 37 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 9, 37 -- MIME-Version: 1.0 9, 37 -- To: Microscopy-at-microscopy.com 9, 37 -- Subject: Re: [Microscopy] ChromaCal 9, 37 -- References: {201402102021.s1AKLFEK006884-at-ns.microscopy.com} 9, 37 -- In-Reply-To: {201402102021.s1AKLFEK006884-at-ns.microscopy.com} 9, 37 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 37 -- Content-Transfer-Encoding: 7bit 9, 37 -- X-Antivirus: avast! (VPS 140211-0, 02/11/2014), Outbound message 9, 37 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both draichle-at-tampabay.rr.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: draichle-at-tampabay.rr.com Name: Dr Erik Raichle
Organization: Raichle Diagnostic
Title-Subject: [Filtered] Specimen Size and The Limits of Lens Optics?
Message: I have 2 questions:
IÂ’ve asked this question of many people, therefore,
About what size would a specimen be when viewed through a microscope at 1000x? 70um?
Will we ever be able to view a specimen at 1000x with a field of view of 1cm and a depth of view of 1cm?
Or, would this exceed the limits of lens of optics?
Also, do we have any other technology beside lens optics that could image a 100um, or less, specimen, such as, sonor or radar or electron beam that could be handheld?
We have identified and lost an extraordinary Mite specimen a person's skin that was less than 100um. It was extraordinary because it looked like a spider, mites that small are wormlike. We believe it is an unclassified pathogen and very much want to capture these specimens on peopleÂ’s skin if we can.
Or perhaps, there is a procedure that will immobilize them and isolate them under a microscope?
Anything you can think of or person who you can refer us to contact, would be most appreciated. Thank you, Dr Erik Raichle
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==============================Original Headers============================== 21, 29 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb 11 10:22:06 2014 21, 29 -- Received: from znl.com ([206.69.208.20]) 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BGM66d004271 21, 29 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 10:22:06 -0600 21, 29 -- Received: from localhost (localhost [127.0.0.1]) 21, 29 -- by znl.com (Postfix) with ESMTP id 789536BA859 21, 29 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 10:22:06 -0600 (CST) 21, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 21, 29 -- Received: from znl.com ([127.0.0.1]) 21, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 21, 29 -- with ESMTP id JLYg9GGlR9Yf for {microscopy-at-microscopy.com} ; 21, 29 -- Tue, 11 Feb 2014 10:22:02 -0600 (CST) 21, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 21, 29 -- by znl.com (Postfix) with ESMTPA id 73F526BA84C 21, 29 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 10:22:02 -0600 (CST) 21, 29 -- Message-ID: {52FA4E2A.2010907-at-microscopy.com} 21, 29 -- Date: Tue, 11 Feb 2014 10:22:02 -0600 21, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 21, 29 -- MIME-Version: 1.0 21, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 29 -- Subject: Fwd: [Filtered] MicroscopyListserverviaWWW:Specimen Size and The 21, 29 -- Limits of Lens Optics? 21, 29 -- References: {201402111551.s1BFpgF2003772-at-ns.microscopy.com} 21, 29 -- In-Reply-To: {201402111551.s1BFpgF2003772-at-ns.microscopy.com} 21, 29 -- X-Forwarded-Message-Id: {201402111551.s1BFpgF2003772-at-ns.microscopy.com} 21, 29 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 29 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I have a customer who needs to measure the coating on 300 micron diameter sodium sulfate core samples coated with ethyl cellulose. The core surface is rough. Customer would like to embed in a suitable resin that will not dissolve the ethyl cellulose coating or cause the coating to shrink/swell. Can someone recommend an embedding media so we can pot and crossection on a microtome for SEM ?
Thank you
Fred Hayes Manager Advanced Materials Characterization and Testing Lab (AMCaT) Dept of Chemical Engineering and Material Science 3001 Ghausi Hall Univ of CA Davis Davis, CA 95616 530-752-0284 chms.engineering.ucdavis.edu/index.html chms.engineering.ucdavis.edu/research/amcat/index.html
==============================Original Headers============================== 6, 22 -- From fahayes-at-ucdavis.edu Tue Feb 11 13:04:53 2014 6, 22 -- Received: from smtp3.ucdavis.edu (smtp3.ucdavis.edu [128.120.32.129]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BJ4r3e025872 6, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 13:04:53 -0600 6, 22 -- Received: from chmsk011201 ([169.237.64.243]) 6, 22 -- by smtp3.ucdavis.edu (8.14.4/8.14.5/it-rhel5-mimedefang-smtp-1.6) with ESMTP id s1BJ4nLA056558 6, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 11:04:50 -0800 6, 22 -- From: "Fred Hayes" {fahayes-at-ucdavis.edu} 6, 22 -- To: {Microscopy-at-microscopy.com} 6, 22 -- Subject: need a recommendation for embedding media 6, 22 -- Date: Tue, 11 Feb 2014 11:04:48 -0800 6, 22 -- Message-ID: {006d01cf275c$23d21a00$6b764e00$-at-ucdavis.edu} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain; 6, 22 -- charset="us-ascii" 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-Mailer: Microsoft Outlook 14.0 6, 22 -- Thread-Index: Ac8nW4CkWM/KhE91R7y6XwCA9Qbaeg== 6, 22 -- Content-Language: en-us 6, 22 -- X-Virus-Scanned: clamav-milter 0.98.1 at smtp3 6, 22 -- X-Virus-Status: Clean 6, 22 -- X-Scanned-By: MIMEDefang 2.74 ==============================End of - Headers==============================
Dr. Raichle; To view a specimen with a 1 cm field of view at 1,000X you would need to have a computer monitor 10 meters across. So it IS possible, just difficult without a Jumbo Tron. Then you would have to stand so far back to see it, that it would get small due to being far away, so what would be the point?
John Mardinly, ASU
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both draichle-at-tampabay.rr.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: draichle-at-tampabay.rr.com Name: Dr Erik Raichle
Organization: Raichle Diagnostic
Title-Subject: [Filtered] Specimen Size and The Limits of Lens Optics?
Message: I have 2 questions:
IÂ've asked this question of many people, therefore,
About what size would a specimen be when viewed through a microscope at 1000x? 70um?
Will we ever be able to view a specimen at 1000x with a field of view of 1cm and a depth of view of 1cm?
Or, would this exceed the limits of lens of optics?
Also, do we have any other technology beside lens optics that could image a 100um, or less, specimen, such as, sonor or radar or electron beam that could be handheld?
We have identified and lost an extraordinary Mite specimen a person's skin that was less than 100um. It was extraordinary because it looked like a spider, mites that small are wormlike. We believe it is an unclassified pathogen and very much want to capture these specimens on peopleÂ's skin if we can.
Or perhaps, there is a procedure that will immobilize them and isolate them under a microscope?
Anything you can think of or person who you can refer us to contact, would be most appreciated. Thank you, Dr Erik Raichle
Login Host: 173.168.136.150 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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==============================Original Headers============================== 27, 43 -- From John.Mardinly-at-asu.edu Tue Feb 11 17:36:54 2014 27, 43 -- Received: from bcnet7.asu.edu (bcnet7.asu.edu [129.219.117.222]) 27, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BNas7o016112 27, 43 -- for {Microscopy-at-Microscopy.com} ; Tue, 11 Feb 2014 17:36:54 -0600 27, 43 -- X-ASG-Debug-ID: 1392161813-04dc7a6a3934990001-FOsErg 27, 43 -- Received: from exhubt01.asurite.ad.asu.edu (exhubt01.asurite.ad.asu.edu [129.219.103.40]) by bcnet7.asu.edu with ESMTP id sK0cUJLL3XCtMgoL (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Tue, 11 Feb 2014 16:36:53 -0700 (MST) 27, 43 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 27, 43 -- X-Barracuda-Apparent-Source-IP: 129.219.103.40 27, 43 -- X-ASG-Whitelist: Client 27, 43 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 27, 43 -- exhubt01.asurite.ad.asu.edu ([129.219.103.40]) with mapi id 14.03.0174.001; 27, 43 -- Tue, 11 Feb 2014 16:36:52 -0700 27, 43 -- From: John Mardinly {John.Mardinly-at-asu.edu} 27, 43 -- To: "microscopylistserver-noreply-at-microscopy.com" 27, 43 -- {microscopylistserver-noreply-at-Microscopy.com} , 27, 43 -- "Microscopy-at-Microscopy.com" 27, 43 -- {Microscopy-at-Microscopy.com} 27, 43 -- Subject: RE: [Microscopy] Fwd: [Filtered] 27, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 27, 43 -- Thread-Topic: [Microscopy] Fwd: [Filtered] 27, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 27, 43 -- X-ASG-Orig-Subj: RE: [Microscopy] Fwd: [Filtered] 27, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 27, 43 -- Thread-Index: AQHPJ0cRbqiyodXEfk+A8PkB3ijSEpqwtAhg 27, 43 -- Date: Tue, 11 Feb 2014 23:36:51 +0000 27, 43 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400FFC0AB2-at-exmbt05.asurite.ad.asu.edu} 27, 43 -- References: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 27, 43 -- In-Reply-To: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 27, 43 -- Accept-Language: en-US 27, 43 -- Content-Language: en-US 27, 43 -- X-MS-Has-Attach: 27, 43 -- X-MS-TNEF-Correlator: 27, 43 -- x-originating-ip: [129.219.103.4] 27, 43 -- Content-Type: text/plain; charset="iso-8859-1" 27, 43 -- MIME-Version: 1.0 27, 43 -- X-Barracuda-Connect: exhubt01.asurite.ad.asu.edu[129.219.103.40] 27, 43 -- X-Barracuda-Start-Time: 1392161813 27, 43 -- X-Barracuda-Encrypted: AES128-SHA 27, 43 -- X-Barracuda-URL: http://129.219.117.222:8000/cgi-mod/mark.cgi 27, 43 -- X-Virus-Scanned: by bsmtpd at asu.edu 27, 43 -- X-Barracuda-BRTS-Status: 1 27, 43 -- Content-Transfer-Encoding: 8bit 27, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1BNas7o016112 ==============================End of - Headers==============================
Dr. Raichle; To view a specimen with a 1 cm field of view at 1,000X you would need to have a computer monitor 10 meters across. So it IS possible, just difficult without a Jumbo Tron. Then you would have to stand so far back to see it, that it would get small due to being far away, so what would be the point?
John Mardinly, ASU
==============================Original Headers============================== 4, 43 -- From John.Mardinly-at-asu.edu Tue Feb 11 17:39:08 2014 4, 43 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 4, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BNd8Um018171 4, 43 -- for {Microscopy-at-Microscopy.com} ; Tue, 11 Feb 2014 17:39:08 -0600 4, 43 -- X-ASG-Debug-ID: 1392161947-06a86756a634050001-FOsErg 4, 43 -- Received: from exhubt01.asurite.ad.asu.edu (exhubt01.asurite.ad.asu.edu [129.219.103.40]) by bcnet8.asu.edu with ESMTP id Lbp4618YAwoIEanC (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Tue, 11 Feb 2014 16:39:07 -0700 (MST) 4, 43 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 43 -- X-Barracuda-Apparent-Source-IP: 129.219.103.40 4, 43 -- X-ASG-Whitelist: Client 4, 43 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 4, 43 -- exhubt01.asurite.ad.asu.edu ([129.219.103.40]) with mapi id 14.03.0174.001; 4, 43 -- Tue, 11 Feb 2014 16:39:07 -0700 4, 43 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 43 -- To: "microscopylistserver-noreply-at-microscopy.com" 4, 43 -- {microscopylistserver-noreply-at-Microscopy.com} , 4, 43 -- "Microscopy-at-Microscopy.com" 4, 43 -- {Microscopy-at-Microscopy.com} 4, 43 -- Subject: RE: [Microscopy] Fwd: [Filtered] 4, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 43 -- Thread-Topic: [Microscopy] Fwd: [Filtered] 4, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 43 -- X-ASG-Orig-Subj: RE: [Microscopy] Fwd: [Filtered] 4, 43 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 43 -- Thread-Index: AQHPJ0cRbqiyodXEfk+A8PkB3ijSEpqwtgRg 4, 43 -- Date: Tue, 11 Feb 2014 23:39:04 +0000 4, 43 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400FFC1ADF-at-exmbt05.asurite.ad.asu.edu} 4, 43 -- References: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 4, 43 -- In-Reply-To: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 4, 43 -- Accept-Language: en-US 4, 43 -- Content-Language: en-US 4, 43 -- X-MS-Has-Attach: 4, 43 -- X-MS-TNEF-Correlator: 4, 43 -- x-originating-ip: [129.219.103.4] 4, 43 -- Content-Type: text/plain; charset="us-ascii" 4, 43 -- MIME-Version: 1.0 4, 43 -- X-Barracuda-Connect: exhubt01.asurite.ad.asu.edu[129.219.103.40] 4, 43 -- X-Barracuda-Start-Time: 1392161947 4, 43 -- X-Barracuda-Encrypted: AES128-SHA 4, 43 -- X-Barracuda-URL: http://129.219.117.210:8000/cgi-mod/mark.cgi 4, 43 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 43 -- X-Barracuda-BRTS-Status: 1 4, 43 -- Content-Transfer-Encoding: 8bit 4, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1BNd8Um018171 ==============================End of - Headers==============================
Dr. Raichle; To view a specimen with a 1 cm field of view at 1,000X you would need to have a computer monitor 10 meters across. So it IS possible, just difficult without a Jumbo Tron. Then you would have to stand so far back to see it, that it would get small due to being far away, so what would be the point?
John Mardinly, ASU
==============================Original Headers============================== 4, 40 -- From John.Mardinly-at-asu.edu Tue Feb 11 17:43:30 2014 4, 40 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BNhUEX029653 4, 40 -- for {Microscopy-at-Microscopy.com} ; Tue, 11 Feb 2014 17:43:30 -0600 4, 40 -- X-ASG-Debug-ID: 1392162208-06a86756a136970001-FOsErg 4, 40 -- Received: from exhubt04.asurite.ad.asu.edu (exhubt04.asurite.ad.asu.edu [129.219.103.43]) by bcnet8.asu.edu with ESMTP id x2NgXb40p3QutSkv (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Tue, 11 Feb 2014 16:43:28 -0700 (MST) 4, 40 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 40 -- X-Barracuda-Apparent-Source-IP: 129.219.103.43 4, 40 -- X-ASG-Whitelist: Client 4, 40 -- Received: from EXMBT05.asurite.ad.asu.edu ([129.219.103.48]) by 4, 40 -- exhubt04.asurite.ad.asu.edu ([129.219.103.43]) with mapi id 14.03.0174.001; 4, 40 -- Tue, 11 Feb 2014 16:43:28 -0700 4, 40 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 40 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 4, 40 -- Subject: RE: [Microscopy] Fwd: [Filtered] 4, 40 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 40 -- Thread-Topic: [Microscopy] Fwd: [Filtered] 4, 40 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 40 -- X-ASG-Orig-Subj: RE: [Microscopy] Fwd: [Filtered] 4, 40 -- MicroscopyListserverviaWWW:Specimen Size and The 4, 40 -- Thread-Index: AQHPJ0cRbqiyodXEfk+A8PkB3ijSEpqwt1MQ 4, 40 -- Date: Tue, 11 Feb 2014 23:43:28 +0000 4, 40 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE9400FFC1B3D-at-exmbt05.asurite.ad.asu.edu} 4, 40 -- References: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 4, 40 -- In-Reply-To: {201402111633.s1BGXunm017847-at-ns.microscopy.com} 4, 40 -- Accept-Language: en-US 4, 40 -- Content-Language: en-US 4, 40 -- X-MS-Has-Attach: 4, 40 -- X-MS-TNEF-Correlator: 4, 40 -- x-originating-ip: [129.219.103.4] 4, 40 -- Content-Type: text/plain; charset="us-ascii" 4, 40 -- MIME-Version: 1.0 4, 40 -- X-Barracuda-Connect: exhubt04.asurite.ad.asu.edu[129.219.103.43] 4, 40 -- X-Barracuda-Start-Time: 1392162208 4, 40 -- X-Barracuda-Encrypted: AES128-SHA 4, 40 -- X-Barracuda-URL: http://129.219.117.210:8000/cgi-mod/mark.cgi 4, 40 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 40 -- X-Barracuda-BRTS-Status: 1 4, 40 -- Content-Transfer-Encoding: 8bit 4, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1BNhUEX029653 ==============================End of - Headers==============================
I hope all is well. If you are interested in advancing your EELS and EFTEM (analytical TEM) knowledge, Gatan is offering a 4 day professional training school on April 8-11, 2014 at its R&D Headquarters in Pleasanton, CA, USA. Here are all the details and online registration: http://www.gatan.com/resources/training/EELSSchoolApr2014.php
EELS & EFTEM Analysis Training School Gatan, Inc. Pleasanton, California, USA April 8-11, 2014
Want to refresh or advance your EELS and EFTEM knowledge? Join us for an intensive 4-day training school that incorporates lectures, computer laboratories, and microscope practicals to provide participants with comprehensive, hands-on training on key EELS and EFTEM topics and technology.
Online registration is now open. Click here {http://www.gatan.com/resources/training/EELSSchoolApr2014.php} . Space is limited.
Overview: Transmission electron microscopy (TEM) reveals details of natural and man-made structures at the micrometer, nanometer, and even sub-nanometer scale. Energy-filtered TEM (EFTEM) and electron energy-loss spectroscopy (EELS) are the ideal analytical partners to the high spatial resolution provided by TEM in both the conventional and scanned (STEM) imaging modes.
This course reviews the basic theory and practice of EELS imaging and analysis in the TEM, but its main emphasis is on practical techniques, optimum deployment of Gatan hardware and software systems, and advanced EELS and EFTEM applications. Some prior experience with EELS, EFTEM, and Gatan systems is recommended, as is a good familiarity with TEM/STEM instrumentation and techniques. By the end of the course, participants can expect to know how best to optimize the performance of their Gatan EELS hardware as well as their EELS and EFTEM experimental setups in order to capture and extract the maximum amount of information from their TEM samples.
Topics
* Fundamentals of EELS and energy-filtered imaging in TEM
* Principles of operation of Gatan EFTEM and EELS systems
* Optimization of EFTEM and EELS data acquisition
* Quantification of elemental composition
* Other information provided by EFTEM/EELS and how best to extract it
* Use of EELS signals to form maps of elemental and chemical composition
* EFTEM and STEM EELS spectrum imaging techniques
* Identification of material phases via EELS fine structure mapping
* Applications to biological and physical science specimens
-- Best regards,
John Marketing Communications Manager
Gatan, Inc.
Email: info-at-gatan.com Website: www.gatan.com
==============================Original Headers============================== 15, 25 -- From JHyun-at-gatan.com Tue Feb 11 17:57:23 2014 15, 25 -- Received: from email.gatan.com (autodiscover.gatan.com [50.58.128.3]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1BNvMqA007937 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 17:57:22 -0600 15, 25 -- Received: from MSE.gatan.com ([fe80::4968:b1cf:a686:47eb]) by email.gatan.com 15, 25 -- ([fe80::5125:aff1:b4a6:ad6e%10]) with mapi id 14.03.0174.001; Tue, 11 Feb 15, 25 -- 2014 15:57:21 -0800 15, 25 -- From: John Hyun {JHyun-at-gatan.com} 15, 25 -- To: Microscopy Server {microscopy-at-microscopy.com} 15, 25 -- Subject: EELS and EFTEM Training School on April 8-11, 2014 15, 25 -- Thread-Topic: EELS and EFTEM Training School on April 8-11, 2014 15, 25 -- Thread-Index: AQHPJ4UAzj47fqt6vEO5uWMdfiSf5w== 15, 25 -- Date: Tue, 11 Feb 2014 23:57:20 +0000 15, 25 -- Message-ID: {CF1FF826.2F8A0%jhyun-at-gatan.com} 15, 25 -- In-Reply-To: {CF0FDB05.2F1FA%jhyun-at-gatan.com} 15, 25 -- Accept-Language: en-US 15, 25 -- Content-Language: en-US 15, 25 -- X-MS-Has-Attach: 15, 25 -- X-MS-TNEF-Correlator: 15, 25 -- x-originating-ip: [10.13.7.151] 15, 25 -- Content-Type: text/plain; charset="us-ascii" 15, 25 -- Content-ID: {46FFE2A191A9A14E9609E8C018C5F06F-at-gatan.com} 15, 25 -- MIME-Version: 1.0 15, 25 -- Content-Transfer-Encoding: 8bit 15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1BNvMqA007937 ==============================End of - Headers==============================
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Message: Upon first turning our newly renovated Philips CM10 TEM, we cannot get any of the functions to work. The standby light on the power supply unit is illuminated as well as the ON button by the display monitor on the electronics rack. When the ON button by the monitor is pressed, the column makes a sort of electronic noise. Other than that there seems to be no power getting to the monitor or any controls. Any idea on what our problem may be? Are there any controls on the power supply that need to be turned on other than the MAINS dial?
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb 11 18:46:39 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C0kdv9027338 12, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:46:39 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id EA1516BACDF 12, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:46:38 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id 7a8Ynqwxeyzi for {microscopy-at-microscopy.com} ; 12, 28 -- Tue, 11 Feb 2014 18:46:37 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id EF9026BACD2 12, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:46:37 -0600 (CST) 12, 28 -- Message-ID: {52FAC46D.3080209-at-microscopy.com} 12, 28 -- Date: Tue, 11 Feb 2014 18:46:37 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:CM10 troubleshooting 12, 28 -- References: {201402111919.s1BJJkeC012484-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201402111919.s1BJJkeC012484-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201402111919.s1BJJkeC012484-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howar Hughes Research Institute
Title-Subject: [Filtered] Re: need a recommendation for embedding media
Message: Back when I was doing materials science samples I always used Epo-Fix. Check the Electron Microscopy Sciences website under Materials Science and Meteorology } Embedding, and there are a lot of new embedding media that may help you.
Best to you,
Lita
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb 11 18:47:28 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C0lSt4028291 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:47:28 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 7E1936BACF0 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:47:28 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id dj4QXx5F0KO2 for {microscopy-at-microscopy.com} ; 14, 28 -- Tue, 11 Feb 2014 18:47:27 -0600 (CST) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id BAE516BACE3 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2014 18:47:27 -0600 (CST) 14, 28 -- Message-ID: {52FAC49F.9040709-at-microscopy.com} 14, 28 -- Date: Tue, 11 Feb 2014 18:47:27 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:need a recommendation for embedding media 14, 28 -- References: {201402111945.s1BJjne6012865-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201402111945.s1BJjne6012865-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201402111945.s1BJjne6012865-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Josh, did you check air pressure (4 to 5 bar) and cooling water flow rate? There are saftey switches which should be activated.
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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==============================Original Headers============================== 7, 22 -- From stefan.diller-at-t-online.de Wed Feb 12 00:44:20 2014 7, 22 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C6iJqg004234 7, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 00:44:19 -0600 7, 22 -- Received: from fwd09.aul.t-online.de (fwd09.aul.t-online.de ) 7, 22 -- by mailout08.t-online.de with smtp 7, 22 -- id 1WDTYP-0001cE-CF; Wed, 12 Feb 2014 07:44:17 +0100 7, 22 -- Received: from mac-pro.speedport.ip (bKTePeZcwh1HeefOCFYr5Td4040Y-9vbkM4a3P0iPK8i9su1SRc5Ee1tF0jfr1+Q-z-at-[84.174.98.43]) by fwd09.t-online.de 7, 22 -- with esmtp id 1WDTYH-1Xt7NQ0; Wed, 12 Feb 2014 07:44:09 +0100 7, 22 -- Message-ID: {52FB183A.60505-at-t-online.de} 7, 22 -- Date: Wed, 12 Feb 2014 07:44:10 +0100 7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 7, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 7, 22 -- MIME-Version: 1.0 7, 22 -- To: microscopy-at-microscopy.com, jcsmtf-at-mail.missouri.edu 7, 22 -- Subject: Re: [Microscopy] viaWWW:CM10 troubleshooting 7, 22 -- References: {201402120052.s1C0q3Kb005834-at-ns.microscopy.com} 7, 22 -- In-Reply-To: {201402120052.s1C0q3Kb005834-at-ns.microscopy.com} 7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- X-ID: bKTePeZcwh1HeefOCFYr5Td4040Y-9vbkM4a3P0iPK8i9su1SRc5Ee1tF0jfr1+Q-z 7, 22 -- X-TOI-MSGID: 87cc288e-c675-489c-af30-4eaafad98669 ==============================End of - Headers==============================
We are currently searching for two SEMs in particular one equipped with a focussed ion beam column and mass spectrometry capabilities. Are any of you aware of manufacturers and does anyone have experience with such instruments?
The formal request for information is available in this link - https://www.ungm.org/Public/Notice/25524
Thanks a lot, Ernesto
Mr Ernesto CHINEA-CANO | Laboratory Assistant | Office of Safeguards Analytical Services | Environmental Sample Laboratory | Department of Safeguards | International Atomic Energy Agency | IAEA Laboratories, Reaktorstrasse 1, A-2444 Seibersdorf, Austria| Email: E.Chinea-Cano-at-iaea.org | T: (+43-1) 2600-28583 | F: (+43-1) 2600-28577| Follow us on www.iaea.org
This email message is intended only for the use of the named recipient. Information contained in this email message and its attachments may be privileged, confidential and protected from disclosure. If you are not the intended recipient, please do not read, copy, use or disclose this communication to others. Also please notify the sender by replying to this message and then delete it from your system.
==============================Original Headers============================== 8, 42 -- From E.Chinea-Cano-at-iaea.org Wed Feb 12 02:18:58 2014 8, 42 -- Received: from vs-m201.iaea.org (vs-m201.iaea.org [161.5.6.178]) 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C8IvRj024502 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 02:18:58 -0600 8, 42 -- Received: from vs-m2.iaea.org (vs-mail2.iaea.org [172.24.1.27]) 8, 42 -- by vs-m201.iaea.org (8.14.3/8.14.3/SuSE Linux 0.8) with ESMTP id s1C8ItBM032619 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 09:18:57 +0100 8, 42 -- Received: from E2.iaea.org (e2.iaea.org [172.24.0.42]) 8, 42 -- by vs-m2.iaea.org (8.14.3/8.14.3/SuSE Linux 0.8) with ESMTP id s1C8ItYs005262 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 09:18:55 +0100 8, 42 -- X-Envelope-Sender: E.Chinea-Cano-at-iaea.org 8, 42 -- Received: from SEM001PD.sg.iaea.org (161.5.105.92) by E2.iaea.org 8, 42 -- (172.24.0.42) with Microsoft SMTP Server (TLS) id 14.1.438.0; Wed, 12 Feb 8, 42 -- 2014 09:18:54 +0100 8, 42 -- Received: from SEM002PD.sg.iaea.org ([161.5.105.93]) by sem001pd.sg.iaea.org 8, 42 -- ([161.5.105.92]) with mapi id 14.01.0438.000; Wed, 12 Feb 2014 09:18:54 +0100 8, 42 -- From: {E.Chinea-Cano-at-iaea.org} 8, 42 -- To: {Microscopy-at-microscopy.com} 8, 42 -- CC: {H.Gronnerod-at-iaea.org} , {N.DZIGAL-at-iaea.org} , {K.Vogt-at-iaea.org} 8, 42 -- Subject: (SEM) The IAEA is seeking manufacturers/authorized agents 8, 42 -- Thread-Topic: (SEM) The IAEA is seeking manufacturers/authorized agents 8, 42 -- Thread-Index: Ac8nyFf48LYAowLSSxKP2KOHn2UcJQ== 8, 42 -- Date: Wed, 12 Feb 2014 08:18:53 +0000 8, 42 -- Message-ID: {2D1B242827F0914FB9F4B77825D49A82C6B829B3-at-sem002pd.sg.iaea.org} 8, 42 -- Accept-Language: en-GB, en-US 8, 42 -- Content-Language: en-US 8, 42 -- X-MS-Has-Attach: 8, 42 -- X-MS-TNEF-Correlator: 8, 42 -- x-originating-ip: [161.5.105.94] 8, 42 -- Content-Type: text/plain; charset="us-ascii" 8, 42 -- MIME-Version: 1.0 8, 42 -- X-TM-AS-Product-Ver: SMEX-10.0.0.1412-7.000.1014-20496.005 8, 42 -- X-TM-AS-Result: No--8.715000-0.000000-31 8, 42 -- X-TM-AS-User-Approved-Sender: Yes 8, 42 -- X-TM-AS-User-Blocked-Sender: No 8, 42 -- X-Anti-Virus: Kaspersky Anti-Virus for Linux Mail Server 5.6.48/RELEASE, 8, 42 -- bases: 20140211 #7507362, check: 20140212 clean 8, 42 -- X-Anti-Virus: Kaspersky Anti-Virus for Linux Mail Server 5.6.48/RELEASE, 8, 42 -- bases: 20140211 #7507362, check: 20140212 clean 8, 42 -- X-Scanned-By: MIMEDefang 2.73 8, 42 -- Content-Transfer-Encoding: 8bit 8, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1C8IvRj024502 ==============================End of - Headers==============================
Following my e-mail in answer to Alejandro Hinojos's question, I was contacted by the DeLonghi representative. So I want just to add some precisions to my former comments.
First, I want to point out that I didn't gave any feedback about the quality of the results that come out of this instrument. To be right and fair, I must say that the tests which were made for us gave pictures in the same quality range than with the STEM device for SEM. As we didn't perform the test ourself, I cannot say anything about the ease of use of the instrument and so on.
Secondly our final choice has ben dictated by to main raisons : -first we have a FE-SEM -second we didn't have the monney for the DeLonghi intrument and no hope to get it in a near future. But we had enough for the STEM device, to be fitted to the existing FE-SEM. The global result is less expend and more flexibility, but less troughput possibility and no self-service use.
It is evident that all situations are not the same. A lab which has no SEM or TEM can have benefit to spend 150 kE in a table-tob TEM/SEM for a fast and easy to use instrument. Or a big EM service may have an interest too, as a tool usefull to sort TEM grids before going to the high end TEM.
I hope that these comments will clarify the contexte of my former mail. My goal was only to point out an other possibility as Alejandro Hinojos said they have soon SEM and TEM in their lab.
Best Regards
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Le 07/02/2014 09:12, jacques.faerber-at-ipcms.u-strasbg.fr a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } } A collegue was interested by this instrument last year and made some } tests. His goal was to have an easy access to a TEM like instrument, to } make fast controls in nanoparticules syntesis (organics with heavy } metals), as the acess to our TEMs need some days of delay. } } Hearing about the price of that instrument, I proposed him to test too a } STEM device to be fitted on our FE-SEM. } Comparing prices and performences (principally the versatility), the } choice was easy. The STEM device for the SEM gives better images, the } possibility to choose the HV up to 30 keV and a price 8x less expensive... } } The real adavantage of this table-top TEM I could see is that it can be } used easy by non-microcopists, like table-top SEMs, with on the other } hand the limitations of the undestanding such people will have of what } they do. } } So if you soon have a (FE)SEM, it could be usefull to consider if the } STEM device for SEM would not be a better solution. But I'm interested } on what your tests will bring you as conclusions. } } Hope it helps } } J. Faerber } IPCMS-DSI } Institut de Physique et Chimie des Matériaux de Strasbourg } Département Surfaces et Interfaces } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail : Jacques.Faerber-at-ipcms.unistra.fr } } Le 07/02/2014 03:28, ahinojos2-at-miners.utep.edu a écrit : } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } Hello Microscopists, } } Here at the University of Texas at El Paso we are searching for a new EM to purchase. We mainly use Hitachi SEM and TEM for Hard materials characterization. We are mainly searching for an EM with specialization in polymers characterization. In our search we stumbled upon the LEVM5 Benchtop TEM from Delong America for polymers. The unit claims to have TEM, SEM, and STEM capabilities. We here in the southwest were wondering what are/were your experiences the LVEM5 or other Delong EM’s? We were also wondering how are the products from the Delong America Company in the perspective of materials characterization? If anybody wouldn't mind sharing those experiences with us that would be great. } } } } } } Thanks for your thoughts and answers in advance } } Alejandro Hinojos } } } } } } } } } } ==============================Original Headers============================== } } 6, 29 -- From ahinojos2-at-miners.utep.edu Thu Feb 6 20:17:12 2014 } } 6, 29 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0181.outbound.protection.outlook.com [207.46.163.181]) } } 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s172HCnn004797 } } 6, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Feb 2014 20:17:12 -0600 } } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com (10.255.210.155) by } } 6, 29 -- BLUPR05MB006.namprd05.prod.outlook.com (10.255.210.142) with Microsoft SMTP } } 6, 29 -- Server (TLS) id 15.0.868.8; Fri, 7 Feb 2014 02:17:10 +0000 } } 6, 29 -- Received: from BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.44]) by } } 6, 29 -- BLUPR05MB008.namprd05.prod.outlook.com ([169.254.2.156]) with mapi id } } 6, 29 -- 15.00.0868.013; Fri, 7 Feb 2014 02:17:10 +0000 } } 6, 29 -- From: "Hinojos, Alejandro" {ahinojos2-at-miners.utep.edu} } } 6, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } } 6, 29 -- Subject: TEM Acquisition Help } } 6, 29 -- Thread-Topic: TEM Acquisition Help } } 6, 29 -- Thread-Index: AQHPI6qIDXb0k8Img0O3WLndhK0Dmg== } } 6, 29 -- Date: Fri, 7 Feb 2014 02:17:10 +0000 } } 6, 29 -- Message-ID: {1e44fc83cbc14812ac71282723f64e06-at-BLUPR05MB008.namprd05.prod.outlook.com} } } 6, 29 -- Accept-Language: en-US } } 6, 29 -- Content-Language: en-US } } 6, 29 -- X-MS-Has-Attach: } } 6, 29 -- X-MS-TNEF-Correlator: } } 6, 29 -- x-originating-ip: [129.108.37.93] } } 6, 29 -- x-forefront-prvs: 011579F31F } } 6, 29 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(199002)(189002)(74876001)(53806001)(90146001)(76482001)(31966008)(74706001)(76796001)(56816005)(76176001)(75432001)(54316002)(92566001)(81816001)(85852003)(87936001)(63696002)(46102001)(76576001)(76786001)(77096001)(51856001)(54356001)(66066001)(65816001)(74502001)(81686001)(74662001)(47446002)(47976001)(2656002)(83322001)(56776001)(81342001)(94946001)(94316002)(87266001)(33646001)(81542001)(47736001)(83072002)(93516002)(74316001)(86362001)(74366001)(50986001)(93136001)(80022001)(69226001)(79102001)(4396001)(59766001)(80976001)(85306002)(49866001)(95416001)(77982001)(24736002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR05MB006;H:BLUPR05MB008.namprd05.prod.outlook.com;CLIP:129.108.37.93;FPR:3ADEF03E.9E339C09.87D2589.84EF8869.201A3;InfoNoRecordsA:0;MX:1;LANG:en; } } 6, 29 -- Content-Type: text/plain; charset="Windows-1252" } } 6, 29 -- MIME-Version: 1.0 } } 6, 29 -- X-OriginatorOrg: miners.utep.edu } } 6, 29 -- Content-Transfer-Encoding: 8bit } } 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s172HCnn004797 } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 10, 25 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Feb 7 02:01:34 2014 } 10, 25 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1781YdX013671 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2014 02:01:34 -0600 } 10, 25 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 10, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id 51B6224238B; } 10, 25 -- Fri, 7 Feb 2014 09:01:52 +0100 (CET) } 10, 25 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr } 10, 25 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) } 10, 25 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) } 10, 25 -- with ESMTP id 458afHncdtNz; Fri, 7 Feb 2014 09:01:51 +0100 (CET) } 10, 25 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) } 10, 25 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id 3652524236C; } 10, 25 -- Fri, 7 Feb 2014 09:01:51 +0100 (CET) } 10, 25 -- Message-ID: {52F492D2.2060601-at-ipcms.u-strasbg.fr} } 10, 25 -- Date: Fri, 07 Feb 2014 09:01:22 +0100 } 10, 25 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } 10, 25 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:17.0) Gecko/20130330 Thunderbird/17.0.5 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: ahinojos2-at-miners.utep.edu, microscopy-at-microscopy.com } 10, 25 -- Subject: Re: [Microscopy] TEM Acquisition Help } 10, 25 -- References: {201402070228.s172SkD0020695-at-ns.microscopy.com} } 10, 25 -- In-Reply-To: {201402070228.s172SkD0020695-at-ns.microscopy.com} } 10, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 26 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Feb 12 02:22:45 2014 11, 26 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1C8MjmP027877 11, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 02:22:45 -0600 11, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 11, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id 54AEC242352 11, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 09:23:14 +0100 (CET) 11, 26 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr 11, 26 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) 11, 26 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) 11, 26 -- with ESMTP id tlzUJTuQtmiX for {microscopy-at-microscopy.com} ; 11, 26 -- Wed, 12 Feb 2014 09:23:13 +0100 (CET) 11, 26 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 11, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id 0BEA524230E 11, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 09:23:13 +0100 (CET) 11, 26 -- Message-ID: {52FB2F5F.6010906-at-ipcms.u-strasbg.fr} 11, 26 -- Date: Wed, 12 Feb 2014 09:22:55 +0100 11, 26 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 11, 26 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:17.0) Gecko/20130330 Thunderbird/17.0.5 11, 26 -- MIME-Version: 1.0 11, 26 -- To: microscopy-at-microscopy.com 11, 26 -- Subject: TEM Acquisition Help : some precisions 11, 26 -- References: {201402070812.s178CgaH028148-at-ns.microscopy.com} 11, 26 -- In-Reply-To: {201402070812.s178CgaH028148-at-ns.microscopy.com} 11, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 26 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
First, is the "Panel Dim" knob pushed in? Turns on the panel. "Data Dim" has to be up to be able to see anything on the monitor. There is a delay while the computer runs self tests. Power supply, left half: The 4 boxes in the top row should all have green lights on when the 'scope is on, regardless of whether or not the panel is on. Same for the 30V "dbl" box in the middle row, and the large black box in the bottom. When the HT button is pushed, the 24V filament box green light will come on in the middle row, as well as the left-most box in that row (no label on our CM-10). There should be no red lights - check the 2 right-most boxes in the middle row. Those are safeties, and have reset buttons. If those are showing red lights, turn off the HT and push the reset buttons.
But. The column shouldn't be making any noise at all. Is it really electronic, or is it a crackly water-flowing noise? Could be air in the cooling lines, which is likely if the 'scope was torn apart. The water lines would need to be disconnected at the exits, and water run through to remove any air in the lines. Were the water lines cleaned with H2O2 or CLR (brand) cleaner? If not, they may be blocked by crud or corrosion. 1 liter 3% H2O2 in the chiller tank for 2 hr - overnight, then 4 or 5 flushes with clean water should clear them.
If it is an electronic noise ... check the connections. If they're OK, then arg. Problem. Get out the sacrificial rooster or undergrad and ...
Phil
} Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10 troubleshooting } } Message: Upon first turning our newly renovated Philips CM10 TEM, we cannot get any of the functions } to work. The standby light on the power supply unit is illuminated as well as the ON button by the } display monitor on the electronics rack. When the ON button by the monitor is pressed, the column } makes a sort of electronic noise. Other than that there seems to be no power getting to the monitor } or any controls. Any idea on what our problem may be? Are there any controls on the power supply } that need to be turned on other than the MAINS dial? -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 29 -- From oshel1pe-at-cmich.edu Wed Feb 12 07:33:01 2014 6, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1CDX1DU005650 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Feb 2014 07:33:01 -0600 6, 29 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 6, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1CDWjpd002219; 6, 29 -- Wed, 12 Feb 2014 08:32:57 -0500 6, 29 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 6, 29 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 6, 29 -- 14.3.174.1; Wed, 12 Feb 2014 08:32:45 -0500 6, 29 -- Message-ID: {52FB77FE.4070107-at-cmich.edu} 6, 29 -- Date: Wed, 12 Feb 2014 08:32:46 -0500 6, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 29 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 29 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 29 -- MIME-Version: 1.0 6, 29 -- To: {jcsmtf-at-mail.missouri.edu} , micro {Microscopy-at-microscopy.com} 6, 29 -- Subject: Re: [Microscopy] viaWWW:CM10 troubleshooting 6, 29 -- References: {201402120102.s1C12SQr030082-at-ns.microscopy.com} 6, 29 -- In-Reply-To: {201402120102.s1C12SQr030082-at-ns.microscopy.com} 6, 29 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- X-Originating-IP: [141.209.160.122] 6, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 29 -- X-Spam-Score: -0.50 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 6, 29 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 29 -- X-CanItPRO-Stream: default 6, 29 -- X-Canit-Stats-ID: 02LppwVdQ - 00d20a283f78 - 20140212 6, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
The Biozentrum of the University of Basel is one of the leading institutes worldwide for molecular and biomedical basic research and teaching. It is home to more than 30 research groups with scientists from over 40 countries. Research at the Biozentrum focuses on the areas of Cell Growth & Development, Infection Biology, Neurobiology, Structural Biology & Biophysics and Computational & Systems Biology. With its more than 550 employees, the Biozentrum is the largest department at the University of Basel’s Faculty of Science.
The University of Basel maintains two sites for electron microscopy, the C-CINA and the ZMB. The Center for Cellular Imaging and NanoAnalytics (C-CINA) performs advanced electron microscopy research in life sciences, see http://c-cina.org. The ZMB provides TEM and SEM services to the University, see http://zmb.unibas.ch. The C-CINA and the ZMB together operate 15 electron microscopes including a FEI Titan Krios with a Gatan K2 Summit direct electron detector, FEI Polara, CM200FEG, Helios FIB-SEM and a Quanta200FEG with a Gatan 3View.
We have an opening for an
========================= EM Service Engineer =========================
Your responsibilities will include the maintenance and repair of all electron microscopes of C-CINA and the ZMB. We do not have any factory maintenance contracts but may request additional help from the factory when needed. Responsibilities of this position are maintenance and repair of the electron microscopes including; problem diagnosis, ordering replacement parts from the factory, and repair. This includes: the Titan Krios Autoloader, cryo-stages, FEG and high voltage supplies, vacuum systems, mechanics, electronics and software backups.
In addition, upon interest, this engineer may participate in electron microscope hardware development projects, which involve hardware design, fabrication in collaboration with our mechanical workshop, construction, and development of electronic control in collaboration with an electronic workshop. Participation in research projects concerning structural biology data collection with these instruments is also possible. C-CINA is a University research group; we develop instruments and methods, and apply them to study the high-resolution 3D structure of biological specimens.
Salary and living conditions in Basel are attractive. The lab language is English.
Your profile Experience in EM service for TEM, ideally also for SEM and FIB. Hardware repair experience.
We offer This is a full-time position permanently funded by the Rektorat of the University of Basel. A highly sought after position in one of the most beautiful countries in Europe. We offer a welcoming working environment in an international and multidisciplinary group covering biochemistry, electron microscopy, structural biology, and data analysis. We are an equal opportunity employer.
Basel is an international city with people from 150 nations. Located at the border where three countries meet - Switzerland-Germany-France. It is Europe’s most important life sciences hub. Basel provides a high standard of living and a rich and varied cultural atmosphere.
For further information, please contact (ABSOLUTE CONFIDENTIALITY IS ASSURED):
Henning Stahlberg, Director Center for Cellular Imaging and NanoAnalytics (C-CINA) Prof. for Structural Biology and Biophysics, Biozentrum, WRO-1058, Mattenstrasse 26 University of Basel, CH-4058 Basel, Switzerland Tel: +41-61-387 32 62 ; http://c-cina.org mailto:Henning.Stahlberg-at-unibas.ch
==============================Original Headers============================== 12, 37 -- From henning.stahlberg-at-unibas.ch Thu Feb 13 04:08:03 2014 12, 37 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 12, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DA82HN011000 12, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 04:08:02 -0600 12, 37 -- Received: from localhost (localhost [127.0.0.1]) 12, 37 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 76E002200EF 12, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 11:08:01 +0100 (CET) 12, 37 -- X-Virus-Scanned: amavisd-new at unibas.ch 12, 37 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 12, 37 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 12, 37 -- with LMTP id 3DgBK0BQ6eNJ for {Microscopy-at-microscopy.com} ; 12, 37 -- Thu, 13 Feb 2014 11:08:01 +0100 (CET) 12, 37 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131]) 12, 37 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 6507E220130 12, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 11:08:01 +0100 (CET) 12, 37 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 12, 37 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id 12, 37 -- 14.03.0174.001; Thu, 13 Feb 2014 11:08:01 +0100 12, 37 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 12, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 37 -- Subject: EM Service Engineer Position available at University Basel, 12, 37 -- Switzerland 12, 37 -- Thread-Topic: EM Service Engineer Position available at University Basel, 12, 37 -- Switzerland 12, 37 -- Thread-Index: AQHPKKN5Os4QjMCcZ0uwm3RcNz3MpQ== 12, 37 -- Date: Thu, 13 Feb 2014 10:08:00 +0000 12, 37 -- Message-ID: {740E1207-B098-4057-A0F4-508C03606617-at-unibas.ch} 12, 37 -- Accept-Language: en-US, de-CH 12, 37 -- Content-Language: en-US 12, 37 -- X-MS-Has-Attach: 12, 37 -- X-MS-TNEF-Correlator: 12, 37 -- x-originating-ip: [129.132.128.85] 12, 37 -- Content-Type: text/plain; charset="Windows-1252" 12, 37 -- Content-ID: {E08389ABE9116644A71EF480521ED231-at-zuv.ads.unibas.ch} 12, 37 -- MIME-Version: 1.0 12, 37 -- Content-Transfer-Encoding: 8bit 12, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1DA82HN011000 ==============================End of - Headers==============================
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Email: mamiller-at-coh.org Name: Marcia M. Miller
Organization: Beckman Research Institute, City of Hope
Title-Subject: [Filtered] Using agar to secure cell pellets
Message: The agar that we are currently using to secure cell pellets is causing a really annoying background all over the sections both inside and outside of cells. This is a terrible graininess that we have seen now in several preps. What we have is bacterial grand from Applichem. We are pretty sure this is the cause of the graininess. Can anyone recommend another agar or another means of securing cell pellets? Sometimes users of our core can provide only small quantities of cells. Thanks in advance for suggestions. Best wishes, Marcia
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We have been using the low-melting-point agarose (NOT agar). It works great. You can find it at Sigma (Cat# A9414).
Zhaojie
Zhaojie Zhang, Ph. D. Director, Jenkins Microscopy Facility University of Wyoming Laramie, WY 82071 PHONE: 307-766-3038 FAX: 307-766-5625
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, February 13, 2014 7:06 AM To: Z.J. Zhang
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Email: mamiller-at-coh.org Name: Marcia M. Miller
Organization: Beckman Research Institute, City of Hope
Title-Subject: [Filtered] Using agar to secure cell pellets
Message: The agar that we are currently using to secure cell pellets is causing a really annoying background all over the sections both inside and outside of cells. This is a terrible graininess that we have seen now in several preps. What we have is bacterial grand from Applichem. We are pretty sure this is the cause of the graininess. Can anyone recommend another agar or another means of securing cell pellets? Sometimes users of our core can provide only small quantities of cells. Thanks in advance for suggestions. Best wishes, Marcia
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 13 08:00:01 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DE00WQ005497 14, 28 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 08:00:00 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 0ACC16BC390 14, 28 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 08:00:00 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id zEptrrs71ss4 for {microscopy-at-microscopy.com} ; 14, 28 -- Thu, 13 Feb 2014 07:59:50 -0600 (CST) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id C55976BC37E 14, 28 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 07:59:50 -0600 (CST) 14, 28 -- Message-ID: {52FCCFD6.10003-at-microscopy.com} 14, 28 -- Date: Thu, 13 Feb 2014 07:59:50 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Using agar to secure cell pellets 14, 28 -- References: {201402122132.s1CLWDdE000365-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201402122132.s1CLWDdE000365-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201402122132.s1CLWDdE000365-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 30, 31 -- From ZZhang-at-uwyo.edu Thu Feb 13 09:20:04 2014 30, 31 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2lp0207.outbound.protection.outlook.com [207.46.163.207]) 30, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DFK4Rb025690 30, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 09:20:04 -0600 30, 31 -- Received: from BN1PR05MB059.namprd05.prod.outlook.com (10.255.202.149) by 30, 31 -- BN1PR05MB059.namprd05.prod.outlook.com (10.255.202.149) with Microsoft SMTP 30, 31 -- Server (TLS) id 15.0.878.16; Thu, 13 Feb 2014 15:19:50 +0000 30, 31 -- Received: from BN1PR05MB059.namprd05.prod.outlook.com ([169.254.11.248]) by 30, 31 -- BN1PR05MB059.namprd05.prod.outlook.com ([169.254.11.248]) with mapi id 30, 31 -- 15.00.0878.008; Thu, 13 Feb 2014 15:19:50 +0000 30, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 30, 31 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 30, 31 -- Subject: RE: [Microscopy] viaWWW:Using agar to secure cell pellets 30, 31 -- Thread-Topic: [Microscopy] viaWWW:Using agar to secure cell pellets 30, 31 -- Thread-Index: AQHPKMTc2eiFVCauLUytXVEz7hk3XpqzShUQ 30, 31 -- Date: Thu, 13 Feb 2014 15:19:49 +0000 30, 31 -- Message-ID: {3d80287025ba4b2ea88c470fbc80541b-at-BN1PR05MB059.namprd05.prod.outlook.com} 30, 31 -- References: {201402131405.s1DE5YX5010524-at-ns.microscopy.com} 30, 31 -- In-Reply-To: {201402131405.s1DE5YX5010524-at-ns.microscopy.com} 30, 31 -- Accept-Language: en-US 30, 31 -- Content-Language: en-US 30, 31 -- X-MS-Has-Attach: 30, 31 -- X-MS-TNEF-Correlator: 30, 31 -- x-originating-ip: [129.72.151.168] 30, 31 -- x-forefront-prvs: 0121F24F22 30, 31 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(51874003)(252514010)(64544002)(199002)(189002)(13464003)(377454003)(63696002)(86362001)(81816001)(33646001)(66066001)(65816001)(80022001)(94946001)(93516002)(79102001)(95416001)(19580405001)(74662001)(74316001)(81686001)(87936001)(74366001)(2656002)(69226001)(93136001)(95666001)(15202345003)(56816005)(87266001)(90146001)(77096001)(15975445006)(80976001)(31966008)(77982001)(94316002)(59766001)(83322001)(47446002)(74502001)(81342001)(85306002)(81542001)(92566001)(85852003)(46102001)(51856001)(53806001)(74876001)(75432001)(49866001)(47736001)(76786001)(4396001)(76576001)(83072002)(15395725003)(47976001)(76796001)(50986001)(19580395003)(54356001)(56776001)(54316002)(74706001)(76482001)(24736002)(80792004)(491001);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR05MB059;H:BN1PR05MB059.namprd05.prod.outlook.com;CLIP:129.72.151.168;FPR:649EF5B5.ACC2913D.3AF1ADBD.40E1A801.2049A;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 30, 31 -- Content-Type: text/plain; charset="us-ascii" 30, 31 -- MIME-Version: 1.0 30, 31 -- X-OriginatorOrg: uwyo.edu 30, 31 -- Content-Transfer-Encoding: 8bit 30, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1DFK4Rb025690 ==============================End of - Headers==============================
Marcia, We have encase small fragile objects between Formvar films on a wire loop. Although this does require appropriate obeissance to the Formvar Spirits, the outcome in terms of structure preservation is excellent. You can read about it in Wu et al. 2012 Nature Protocols 7: 1113- 1124. (I can send you a pdf off line if you are interested). That paper describes using the method on a small plant root but there is no reason in principle why it would not work for a cell pellet. Probably you won't want to switch horses like this but sending a long the info in case you are interested. Good luck! Tobias
} l } ---------------------------------------------------------------------------- } } X-from: mamiller-at-coh.org () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mamiller-at-coh.org as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: mamiller-at-coh.org } Name: Marcia M. Miller } } Organization: Beckman Research Institute, City of Hope } } Title-Subject: [Filtered] Using agar to secure cell pellets } } Message: The agar that we are currently using to secure cell pellets } is causing a really annoying } background all over the sections both inside and outside of cells. } This is a terrible graininess } that we have seen now in several preps. What we have is bacterial } grand from Applichem. We are } pretty sure this is the cause of the graininess. Can anyone } recommend another agar or another means } of securing cell pellets? Sometimes users of our core can provide } only small quantities of cells. } Thanks in advance for suggestions. Best wishes, Marcia } } Login Host: 151.152.101.44 } Listserver Email Form V - 20120416 } ---------------------------------------------------------------------------
-- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Professor /_ / __ /__ \ \ \__ Biology Department / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / ___ / \ \__/ \ ____ Amherst, MA, 01003 www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 4, 21 -- From baskin-at-bio.umass.edu Thu Feb 13 09:28:29 2014 4, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DFSS9V001391 4, 21 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 09:28:29 -0600 4, 21 -- Received: from [172.30.52.170] (neutopia.bio.umass.edu [128.119.55.8]) 4, 21 -- (authenticated bits=0) 4, 21 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id s1DFSP7H007665 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 21 -- Thu, 13 Feb 2014 10:28:26 -0500 (EST) 4, 21 -- Mime-Version: 1.0 4, 21 -- Message-Id: {p06240505cf22939825da-at-[172.30.52.170]} 4, 21 -- In-Reply-To: {201402131400.s1DE0atb006103-at-ns.microscopy.com} 4, 21 -- References: {201402131400.s1DE0atb006103-at-ns.microscopy.com} 4, 21 -- Date: Thu, 13 Feb 2014 10:28:24 -0500 4, 21 -- To: microscopy-at-microscopy.com 4, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 4, 21 -- Subject: Re: [Microscopy] viaWWW:Using agar to secure cell pellets 4, 21 -- Cc: mamiller-at-coh.org 4, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Thu, 13 Feb 2014 10:28:26 -0500 (EST) 4, 21 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
We use 1.5% agarose in PBS (I assume other medium works too). We first mix the agarose with PBS in an eppendorf tube, then immerse the tube in boiling water for a few minutes. The agarose would be completed 'melted' at this point. We then transfer the tube to a 37 degree C water bath, to let it cool down to 37C. The agarose (melting point 36C) will stay melted at 37C.
We then mixed 1 part of cells and 1 part of agarose at 37C, then bring the mixture to room temperature. The mixture will become solid right away (no need for ice)!
Hope this helps.
Zhaojie
X-from: Erin Tranfield [mailto:etranfield-at-igc.gulbenkian.pt] Sent: Thursday, February 13, 2014 8:57 AM To: Z.J. Zhang
Hi Marcia:
We have been using the low-melting-point agarose (NOT agar). It works great. You can find it at Sigma (Cat# A9414).
Zhaojie
Zhaojie Zhang, Ph. D. Director, Jenkins Microscopy Facility University of Wyoming Laramie, WY 82071 PHONE: 307-766-3038 FAX: 307-766-5625
==============================Original Headers============================== 30, 33 -- From ZZhang-at-uwyo.edu Thu Feb 13 10:15:00 2014 30, 33 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2lp0206.outbound.protection.outlook.com [207.46.163.206]) 30, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DGF0LG031702 30, 33 -- for {microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 10:15:00 -0600 30, 33 -- Received: from BN1PR05MB059.namprd05.prod.outlook.com (10.255.202.149) by 30, 33 -- BN1PR05MB060.namprd05.prod.outlook.com (10.255.202.153) with Microsoft SMTP 30, 33 -- Server (TLS) id 15.0.868.8; Thu, 13 Feb 2014 16:14:46 +0000 30, 33 -- Received: from BN1PR05MB059.namprd05.prod.outlook.com ([169.254.11.248]) by 30, 33 -- BN1PR05MB059.namprd05.prod.outlook.com ([169.254.11.248]) with mapi id 30, 33 -- 15.00.0878.008; Thu, 13 Feb 2014 16:14:46 +0000 30, 33 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 30, 33 -- To: "'Erin Tranfield'" {etranfield-at-igc.gulbenkian.pt} 30, 33 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 30, 33 -- Subject: RE: Guidance with LMP Agarose and Cell Pellets? 30, 33 -- Thread-Topic: Guidance with LMP Agarose and Cell Pellets? 30, 33 -- Thread-Index: AQHPKNRY0HFH/s8gp0C7z9/Nv0rptpqzW6bQ 30, 33 -- Date: Thu, 13 Feb 2014 16:14:45 +0000 30, 33 -- Message-ID: {95688f6dd3dd453d9a662c699413d7c6-at-BN1PR05MB059.namprd05.prod.outlook.com} 30, 33 -- References: {201402131537.s1DFbGp9021382-at-ns.microscopy.com} 30, 33 -- {AF9E7A88-93B4-453B-806D-9938EDC5F521-at-igc.gulbenkian.pt} 30, 33 -- In-Reply-To: {AF9E7A88-93B4-453B-806D-9938EDC5F521-at-igc.gulbenkian.pt} 30, 33 -- Accept-Language: en-US 30, 33 -- Content-Language: en-US 30, 33 -- X-MS-Has-Attach: 30, 33 -- X-MS-TNEF-Correlator: 30, 33 -- x-originating-ip: [129.72.151.168] 30, 33 -- x-forefront-prvs: 0121F24F22 30, 33 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(252514010)(48214007)(377454003)(129404003)(199002)(189002)(24454002)(81686001)(80976001)(74316001)(76786001)(76796001)(69226001)(74876001)(74706001)(74366001)(56776001)(87266001)(54316002)(76482001)(85852003)(2656002)(4396001)(94946001)(46102001)(83072002)(81342001)(66066001)(76576001)(85306002)(81542001)(87936001)(80022001)(95666001)(65816001)(81816001)(93516002)(77096001)(74662001)(86362001)(47446002)(15975445006)(31966008)(47736001)(92566001)(49866001)(15202345003)(63696002)(90146001)(59766001)(56816005)(77982001)(83322001)(47976001)(50986001)(79102001)(53806001)(19580395003)(94316002)(19580405001)(54356001)(95416001)(51856001)(33646001)(74502001)(93136001)(75432001)(24736002)(80792004);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR05MB060;H:BN1PR05MB059.namprd05.prod.outlook.com;CLIP:129.72.151.168;FPR:E45EF58D.A62AA7CD.39F91D97.C0F5CC3B.20440;InfoNoRecordsA:1;MX:1;LANG:en; 30, 33 -- Content-Type: text/plain; charset="iso-8859-1" 30, 33 -- MIME-Version: 1.0 30, 33 -- X-OriginatorOrg: uwyo.edu 30, 33 -- Content-Transfer-Encoding: 8bit 30, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1DGF0LG031702 ==============================End of - Headers==============================
Dear All, here are some images from a Raspberry leaf: http://www.electronmicroscopy.info/sem_images/index.htm?1 Can someone please help me identify the structures seen in the mid and right image? Especially those with the small "spheres" on the surface? Is this mostly mold on the surface?
Best wishes, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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realname - Anne Howson Email - AKHPhoto-at-pacbell.net ORGANIZATION - Richardson Bay Audubon Center EDUCATION - Graduate College LOCATION - Tiburon, Ca. QUESTION - We have an Olympus SZ61 Microscope with an Hitachi CCD color camera (model kP-D20BU attached which we connect to a projector to teach or docents and trainees about plankton in San Francisco Bay. Several months ago the system stopped projecting color images and will now only project black & white. This is not nearly as effective and dramatic. Can you suggest what might be wrong? We tried buying a new projector, but that did not fix the problem. Thanks for any help you can give us. As a small non-profit educational facility, we do not have much $.
==============================Original Headers============================== 2, 27 -- From oshel1pe-at-cmich.edu Thu Feb 13 15:44:37 2014 2, 27 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DLiafg009363 2, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 15:44:36 -0600 2, 27 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75] (may be forged)) 2, 27 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1DLbRtm003872 2, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 16:37:32 -0500 2, 27 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 2, 27 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 2, 27 -- 14.3.174.1; Thu, 13 Feb 2014 16:11:48 -0500 2, 27 -- Message-ID: {52FD3512.3080000-at-cmich.edu} 2, 27 -- Date: Thu, 13 Feb 2014 16:11:46 -0500 2, 27 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 2, 27 -- Reply-To: {oshel1pe-at-cmich.edu} 2, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 2, 27 -- MIME-Version: 1.0 2, 27 -- To: micro {Microscopy-at-microscopy.com} 2, 27 -- Subject: Ask-A-Microscopist Problems with Olympus CCD camera on light microscope 2, 27 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 2, 27 -- Content-Transfer-Encoding: 7bit 2, 27 -- X-Originating-IP: [141.209.160.122] 2, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 27 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 2, 27 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 27 -- X-CanItPRO-Stream: default 2, 27 -- X-Canit-Stats-ID: 05LpVBwMn - 10ee1c4fa5f8 - 20140213 2, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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You should be able to get excellent in-person help from the San Francisco Microscopical Society, http://sfmicrosoc.org They're a mix of professional and amateur microscopists in the SF Bay area who are dedicated to the sort of educational outreach that you're doing. -- Caroline Schooley Project MICRO Co-Chair Microscopy Society of America 45301 Caspar Point Road, Box 117 Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/education/projectMICRO
==============================Original Headers============================== 2, 19 -- From schooley-at-mcn.org Thu Feb 13 16:35:19 2014 2, 19 -- Received: from smtp3.mcn.org (smtp3.mcn.org [216.150.240.89]) 2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1DMZJY1028772 2, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Feb 2014 16:35:19 -0600 2, 19 -- Received: from [66.81.52.152] 2, 19 -- by smtp3.mcn.org with esmtpa (Exim 4.82) 2, 19 -- (envelope-from {schooley-at-mcn.org} ) 2, 19 -- id 1WE4sF-00062H-5v; Thu, 13 Feb 2014 14:35:18 -0800 2, 19 -- Mime-Version: 1.0 2, 19 -- Message-Id: {a06240801cf22f6bea43a-at-[66.81.52.152]} 2, 19 -- In-Reply-To: {201402132153.s1DLreFE018372-at-ns.microscopy.com} 2, 19 -- References: {201402132153.s1DLreFE018372-at-ns.microscopy.com} 2, 19 -- Date: Thu, 13 Feb 2014 14:35:06 -0800 2, 19 -- To: Anne Howson {AKHPhoto-at-pacbell.net} 2, 19 -- From: Caroline Schooley {schooley-at-mcn.org} 2, 19 -- Subject: Re: [Microscopy] Ask-A-Microscopist Problems with Olympus CCD 2, 19 -- camera on light microscope 2, 19 -- Cc: Microscopy-at-microscopy.com 2, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Hello All, Please, could anybody give us an advice or hint on following problem? We have a trouble with our Philips CM100. I can see the light on main screen but cannot focus the beam with Intensity button. At both end positions of Intensity button range I can hear the beeps. Microscope restart did not help. Without any touching the Intensity button C2 Condenser current in LM mode is oscillating between 3900 mA and 3924 mA. In M mode it is oscillating from 3868 mA to 3944 mA. C1 Condenser currents seem to be OK (389, 424, 470, 550, 640, 748, 855,1026, 1266, 1486, 2166 mA for spotsize from 1 to 11).
Thanking you in advance.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR. v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 4, 37 -- From benada-at-biomed.cas.cz Fri Feb 14 04:16:55 2014 4, 37 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1EAGtMe023859 4, 37 -- for {Microscopy-at-Microscopy.com} ; Fri, 14 Feb 2014 04:16:55 -0600 4, 37 -- X-ASG-Debug-ID: 1392373006-05011e01d2048a0001-FOsErg 4, 37 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id lLnCFVOo3ehWjmrK (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO) for {Microscopy-at-Microscopy.com} ; Fri, 14 Feb 2014 11:16:46 +0100 (CET) 4, 37 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 4, 37 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 4, 37 -- Received: from u117fs (c111kh.mbu.cas.cz [147.231.44.14]) 4, 37 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 4, 37 -- (No client certificate requested) 4, 37 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 60AEE2C8472 4, 37 -- for {Microscopy-at-Microscopy.com} ; Fri, 14 Feb 2014 11:16:46 +0100 (CET) 4, 37 -- Date: Fri, 14 Feb 2014 11:16:44 +0100 4, 37 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 4, 37 -- To: Microscopy-at-Microscopy.com 4, 37 -- Subject: CM100 C2 problem 4, 37 -- Message-ID: {20140214111644.36e0f807-at-u117fs} 4, 37 -- X-ASG-Orig-Subj: CM100 C2 problem 4, 37 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 4, 37 -- =?UTF-8?B?xIxS?= 4, 37 -- X-Mailer: Claws Mail 3.9.3 (GTK+ 2.24.10; i486-pc-linux-gnu) 4, 37 -- MIME-Version: 1.0 4, 37 -- Content-Type: text/plain; charset=US-ASCII 4, 37 -- Content-Transfer-Encoding: 7bit 4, 37 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 4, 37 -- X-Barracuda-Start-Time: 1392373006 4, 37 -- X-Barracuda-Encrypted: AES256-SHA 4, 37 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 4, 37 -- X-Barracuda-BRTS-Status: 1 4, 37 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 4, 37 -- X-Barracuda-Spam-Score: 0.00 4, 37 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 4, 37 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.145103 4, 37 -- Rule breakdown below 4, 37 -- pts rule name description 4, 37 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
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realname - David Glick Email - glickdavidb-at-gmail.com ORGANIZATION - San Joaquin Delta College EDUCATION - Undergraduate College LOCATION - Stockton Ca. USA SUBJECT_OF_QUESTION - Level of Education QUESTION - I am currently working towards a two year certificate in EM and considering continuing my education on a part-time basis. My question is two part: First; If I am considering working in the materials/crystalline sector how far can I expect to get with just a certificate? Second; Does obtaining a degree above a bachelors improve upward mobility in the industry? The Reason I ask these questions is that I am a 45 year old re-entry student and do not want to needlessly spend time and money in a university if it won't improve my employment standard.
==============================Original Headers============================== 4, 28 -- From oshel1pe-at-cmich.edu Fri Feb 14 07:02:23 2014 4, 28 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1ED2NE8012132 4, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 07:02:23 -0600 4, 28 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75] (may be forged)) 4, 28 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1ECsueu014469 4, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 07:55:19 -0500 4, 28 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 4, 28 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 28 -- 14.3.174.1; Fri, 14 Feb 2014 08:02:07 -0500 4, 28 -- Message-ID: {52FE13D7.2030406-at-cmich.edu} 4, 28 -- Date: Fri, 14 Feb 2014 08:02:15 -0500 4, 28 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 4, 28 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 28 -- MIME-Version: 1.0 4, 28 -- To: micro {Microscopy-at-microscopy.com} 4, 28 -- Subject: Ask-A-Microscopist How helpful are advanced degrees in getting microscopy 4, 28 -- jobs? 4, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 28 -- Content-Transfer-Encoding: 7bit 4, 28 -- X-Originating-IP: [141.209.160.122] 4, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 28 -- X-Spam-Score: 0.20 () [Hold at 6.00] RDNS_NONE,_L_IAA,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 28 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 28 -- X-CanItPRO-Stream: default 4, 28 -- X-Canit-Stats-ID: 05LqcTj86 - f70fc822a112 - 20140214 4, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
In my 40 years of chemistry and microscopy I've seen changes. Today industry hires BS to do the work technician did 30 years ago. 60 years ago you might have gotten an interview for a position as a technician but now you don't get in the door with out your sheep skin. I took my BS degree in Chemistry and scrambled to take microscopy courses at McCrone and other places. I found a position as a Microscopist and never looked back.
I worked for a man who had a PhD in the metallography of welding, He was moving up the management chain powdered by his drive and the PhD. So yes, I would say a degree is better than no degree. An advanced degree is better than a BS, but pick your field with care. Add other skill sets, like programming, electronics to round out your skill set.
Nothing is guarantied. I have a friend with a BS in chemical engineering and a masters in chemistry. He's in law school because he couldn't find a job in those fields. But he will not leave California either.......
If it was a perfect world, you could try to find a position which would allow you to finish school while employed. My limited experience is you then need to change job as soon as possible because management will always see you as a technician.
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Friday, February 14, 2014 8:12 AM To: Frank Karl
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realname - David Glick Email - glickdavidb-at-gmail.com ORGANIZATION - San Joaquin Delta College EDUCATION - Undergraduate College LOCATION - Stockton Ca. USA SUBJECT_OF_QUESTION - Level of Education QUESTION - I am currently working towards a two year certificate in EM and considering continuing my education on a part-time basis. My question is two part: First; If I am considering working in the materials/crystalline sector how far can I expect to get with just a certificate? Second; Does obtaining a degree above a bachelors improve upward mobility in the industry? The Reason I ask these questions is that I am a 45 year old re-entry student and do not want to needlessly spend time and money in a university if it won't improve my employment standard.
==============================Original Headers============================== 4, 28 -- From oshel1pe-at-cmich.edu Fri Feb 14 07:02:23 2014 4, 28 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1ED2NE8012132 4, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 07:02:23 -0600 4, 28 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75] (may be forged)) 4, 28 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1ECsueu014469 4, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 07:55:19 -0500 4, 28 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 4, 28 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 28 -- 14.3.174.1; Fri, 14 Feb 2014 08:02:07 -0500 4, 28 -- Message-ID: {52FE13D7.2030406-at-cmich.edu} 4, 28 -- Date: Fri, 14 Feb 2014 08:02:15 -0500 4, 28 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 4, 28 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 28 -- MIME-Version: 1.0 4, 28 -- To: micro {Microscopy-at-microscopy.com} 4, 28 -- Subject: Ask-A-Microscopist How helpful are advanced degrees in getting microscopy 4, 28 -- jobs? 4, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 28 -- Content-Transfer-Encoding: 7bit 4, 28 -- X-Originating-IP: [141.209.160.122] 4, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 28 -- X-Spam-Score: 0.20 () [Hold at 6.00] RDNS_NONE,_L_IAA,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 28 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 28 -- X-CanItPRO-Stream: default 4, 28 -- X-Canit-Stats-ID: 05LqcTj86 - f70fc822a112 - 20140214 4, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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==============================Original Headers============================== 19, 29 -- From frank_karl-at-ardl.com Fri Feb 14 08:50:01 2014 19, 29 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1EEo1Tt032708 19, 29 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 08:50:01 -0600 19, 29 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 19, 29 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Fri, 14 19, 29 -- Feb 2014 09:50:00 -0500 19, 29 -- From: Frank Karl {frank_karl-at-ardl.com} 19, 29 -- To: "'glickdavidb-at-gmail.com'" {glickdavidb-at-gmail.com} 19, 29 -- CC: "Microscopy Listserver (microscopy-at-microscopy.com)" 19, 29 -- {microscopy-at-microscopy.com} 19, 29 -- Date: Fri, 14 Feb 2014 09:49:59 -0500 19, 29 -- Subject: RE: [Microscopy] Ask-A-Microscopist How helpful are advanced 19, 29 -- degrees in getting microscopy 19, 29 -- Thread-Topic: [Microscopy] Ask-A-Microscopist How helpful are advanced 19, 29 -- degrees in getting microscopy 19, 29 -- Thread-Index: Ac8phmvKTolTGrT9TkKDtZE6GyLKlgAC2IUg 19, 29 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BD05BDE5D-at-exchange2k7.ad.ardl.com} 19, 29 -- References: {201402141311.s1EDBgFX025803-at-ns.microscopy.com} 19, 29 -- In-Reply-To: {201402141311.s1EDBgFX025803-at-ns.microscopy.com} 19, 29 -- Accept-Language: en-US 19, 29 -- Content-Language: en-US 19, 29 -- X-MS-Has-Attach: 19, 29 -- X-MS-TNEF-Correlator: 19, 29 -- acceptlanguage: en-US 19, 29 -- Content-Type: text/plain; charset="us-ascii" 19, 29 -- MIME-Version: 1.0 19, 29 -- Content-Transfer-Encoding: 8bit 19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1EEo1Tt032708 ==============================End of - Headers==============================
I am trying to hone my understanding of diffraction in microscopy: particularly the slit diffraction analogy and how diffraction affects resolution in microscopy. 1. If you take a sub-resolution point source of light (GFP molecule) and image it through an objective, you get a central maximum and a series of concentric disks due to diffraction during image formation. Question- What is the source of the diffraction? There are no slit like openings that are less than the wavelength of the light. I am presuming this diffraction due light diffracting at the edges of the objective? (I could not find a good written/diagrammatic description of the issue- can someone point me to one?)
2. The separation of Airy disk central maxima as an explanation for resolution makes some intuitive sense, but is only taking account of the NA of the objective as if there are no other sources of diffraction other than the objective. This would be reasonable for a purified fluorescent molecule/protein attached to a coverslip. However, in cells, you now add many other sources of diffraction as light passes into and out of a cell. So would it be more correct to say that the calculated resolution is a maximal or optimal resolution? I am presuming that this is routinely not acheived in cell/tissue imaging? Do we have an estimate of how much resolution is lost by a molecule being in the middle of a cell (ie. what you get from a single superresolution activated molecule in PALM) as compared to a purified molecules in isolation? Presumably you can back calculate this from the actual diffraction pattern measured for single activated molecules in a cell vs. in isolation? Thanks- Dave
David Knecht, Ph.D. Professor and Head of Core Microscopy Facility Department of Molecular and Cell Biology U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 9, 27 -- From david.knecht-at-uconn.edu Fri Feb 14 09:22:32 2014 9, 27 -- Received: from exchange.uconn.edu (cashuba.grove.ad.uconn.edu [137.99.30.165]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1EFMWEM019670 9, 27 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 09:22:32 -0600 9, 27 -- Received: from CASHUBH.grove.ad.uconn.edu (137.99.203.212) by 9, 27 -- CashubA.grove.ad.uconn.edu (137.99.30.165) with Microsoft SMTP Server (TLS) 9, 27 -- id 14.3.174.1; Fri, 14 Feb 2014 10:22:31 -0500 9, 27 -- Received: from MAILD.grove.ad.uconn.edu ([169.254.5.33]) by 9, 27 -- CASHUBH.grove.ad.uconn.edu ([137.99.203.212]) with mapi id 14.03.0174.001; 9, 27 -- Fri, 14 Feb 2014 10:22:31 -0500 9, 27 -- From: "Knecht, David" {david.knecht-at-uconn.edu} 9, 27 -- To: microscopy microscopy {microscopy-at-microscopy.com} 9, 27 -- Subject: Microscopy basics- airy disks and fluorescence resolution 9, 27 -- Thread-Topic: Microscopy basics- airy disks and fluorescence resolution 9, 27 -- Thread-Index: AQHPKZiUBUJwaeBxdU6ZZQ55NOMqcA== 9, 27 -- Date: Fri, 14 Feb 2014 15:22:31 +0000 9, 27 -- Message-ID: {4B6EB19C-C087-43E5-B386-07302688F655-at-uconn.edu} 9, 27 -- Accept-Language: en-US 9, 27 -- Content-Language: en-US 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- x-originating-ip: [137.99.47.75] 9, 27 -- Content-Type: text/plain; charset="us-ascii" 9, 27 -- Content-ID: {0E90464930A8774AB4703B604722335D-at-ad.uconn.edu} 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1EFMWEM019670 ==============================End of - Headers==============================
1) Actually the Young’s slit experiment doesn’t require the width or the distance between the slits to be less than the wavelength of light. You can do it using a slide that you’ve put a thin black coating on (using model airplane paint for example) and then scratch it twice with a razor and shine a light through it. If you do your trigonometry, you’ll find out that if you put the slits really close (as in a few hundred microns or even a mm) you can see the diffraction pattern pretty clearly some feet away. In a microscope, you have apertures, and tubes bounding the light path and that is what is causing your diffraction. If you are operating at high NA then often the limiting aperture is the objective. You’ll get diffraction whenever the light path from one side of the aperture is on the order of one wavelength of light different than in the middle of the aperture, practically speaking, high mag.
2) Point resolution limits described by airy disks etc are really rules of thumb. As you note, the resolution limit is the smallest feature you can separate from your image, nothing more or less. Usually that is much less than the Airy resolution due to inhomogeneities in the sample, like you describe. On the other hand, theoretically, if you know EXACTLY the diffraction characteristics of your entire system, and your sample is composed of point sources, and you have infinite signal to noise, you can separate two different points that are infinitely close together. You can make a quick demonstration with Matlab or a similar program by plotting two Gaussians with FWHM of 100 units, and centroids separated by 1 unit, and then do a fit to extract the separation between the two centroids. You won’t have any trouble even though this scenario is resolving peaks far, far below the "resolution limit." As soon as you add even a little noise, you cannot make the fit work. As soon as you allow for any uncertainty in the FWHM or skewness of the peaks, then you also can’t get a good number from your fit. I encourage you to try this, actually, it is very informative just how little noise or uncertainty in the peak shapes is required to destroy your ability to resolve the two peaks, and if you play with it for a while will wind up deriving a practical resolution limit “law” of your own. Chances are you will wind up with a limit not far from the FWHM of the Gaussians. If you like programming you can do this with Airy disks and you’ll “reinvent” the Airy resolution limit. In a real microscope, you always have some aberrations, and your sample always has some inhomogeneities (otherwise why are you looking at it?). So the Airy resolution is a very practical limit and very useful, but there is no deep fundamental reason why the grand master of the universe says that’s the resolution limit. It’s just a good demarcation that was chosen based on a reasonable application of diffraction through an aperture.
I hope this helps!
Zack Gainsforth Space Sciences Laboratory, UC Berkeley 7 Gauss Way Berkeley, CA 94720 cell: 626-437-9186(tel://cell:%20626-437-9186) zackg-at-ssl.berkeley.edu(mailto:zackg-at-ssl.berkeley.edu)
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Message: Upon turning on our CM10 for the first time after the install there is a message saying "Pneumatics". I can't seem to get the ODP to turn on, and twice now the viewing glass has been pushed out by the compressed air. Does anyone have any ideas on what the problem may be?
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Message: I have not been able to activate the HIVAC and UHV on our CM10. The pressures of the pironi gauges are: P1=82, P2=19, P3=0, IGP=0. This is after the pre-vavc has been running for roughly 20 minutes. Once the pre-vac is turned off P2 goes to about ~70. Is it normal to take longer than this to get the P2 down below 13.3?
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 14 16:51:00 2014 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1EMp0Ri004534 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 16:51:00 -0600 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 76A9D6BD4AF 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 16:51:00 -0600 (CST) 11, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id rElzRzX1XjnU for {microscopy-at-microscopy.com} ; 11, 28 -- Fri, 14 Feb 2014 16:50:59 -0600 (CST) 11, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 2AC766BD4A2 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 16:50:59 -0600 (CST) 11, 28 -- Message-ID: {52FE9DD3.1080205-at-microscopy.com} 11, 28 -- Date: Fri, 14 Feb 2014 16:50:59 -0600 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:CM10 HIVAC 11, 28 -- References: {201402142006.s1EK6E8d000836-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201402142006.s1EK6E8d000836-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201402142006.s1EK6E8d000836-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A "pneumatics" error message generally means that your air pressure is too low to operate the valves of the microscope. The FEI TEMs generally want to have ~6 bar air pressure.
If your viewing window is being forced out, it sounds like you may have a more serious problem than the pneumatics error. The viewing chamber is not supposed to have a positive pressure but be under vacuum. Do you have a pressurized air supply hooked to the camera vent valve (valve V12 on the vacuum schematic)? It's possible that you have a leaky valve.
Cheers, Henk
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==============================Original Headers============================== 7, 47 -- From colijn.1-at-osu.edu Fri Feb 14 21:08:59 2014 7, 47 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1ehsobe005.messaging.microsoft.com [216.32.181.185]) 7, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1F38vET012741 7, 47 -- for {microscopy-at-microscopy.com} ; Fri, 14 Feb 2014 21:08:59 -0600 7, 47 -- Received: from mail108-ch1-R.bigfish.com (10.43.68.228) by 7, 47 -- CH1EHSOBE021.bigfish.com (10.43.70.78) with Microsoft SMTP Server id 7, 47 -- 14.1.225.22; Sat, 15 Feb 2014 03:08:56 +0000 7, 47 -- Received: from mail108-ch1 (localhost [127.0.0.1]) by 7, 47 -- mail108-ch1-R.bigfish.com (Postfix) with ESMTP id 9423024031B for 7, 47 -- {microscopy-at-microscopy.com} ; Sat, 15 Feb 2014 03:08:56 +0000 (UTC) 7, 47 -- X-Forefront-Antispam-Report: CIP:164.107.81.210;KIP:(null);UIP:(null);IPV:NLI;H:cio-krc-pf03;RD:none;EFVD:NLI 7, 47 -- X-SpamScore: 2 7, 47 -- X-BigFish: VPS2(zzbb2dI62a3I98dI9371I1432Izz1f42h2148h1d77h208ch1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch1fc6hzd9hz1de098h177df4h17326ah8275bh1de097h186068hz2fh2a8h839h947hd25he5bhf0ah1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh162dh1631h1758h1765h18e1h190ch1946h19b4h19c3h19ceh1ad9h1b0ah224fh1d0ch1d2eh1d3fh1dfeh1dffh1f5fh1fe8h1ff5h209eh22d0h2336h2438h2461h2476h2487h24d7h2516h2545h255eh1b1cn1b1bi1155h) 7, 47 -- Received-SPF: pass (mail108-ch1: domain of osu.edu designates 164.107.81.210 as permitted sender) client-ip=164.107.81.210; envelope-from=colijn.1-at-osu.edu; helo=cio-krc-pf03 ;cio-krc-pf03 ; 7, 47 -- Received: from mail108-ch1 (localhost.localdomain [127.0.0.1]) by mail108-ch1 7, 47 -- (MessageSwitch) id 1392433734796166_15640; Sat, 15 Feb 2014 03:08:54 +0000 7, 47 -- (UTC) 7, 47 -- Received: from CH1EHSMHS024.bigfish.com (snatpool1.int.messaging.microsoft.com 7, 47 -- [10.43.68.242]) by mail108-ch1.bigfish.com (Postfix) with ESMTP id 7, 47 -- B49BB1000DB; Sat, 15 Feb 2014 03:08:54 +0000 (UTC) 7, 47 -- Received: from cio-krc-pf03 (164.107.81.210) by CH1EHSMHS024.bigfish.com 7, 47 -- (10.43.70.24) with Microsoft SMTP Server (TLS) id 14.16.227.3; Sat, 15 Feb 7, 47 -- 2014 03:08:54 +0000 7, 47 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) (using TLSv1 7, 47 -- with cipher RC4-MD5 (128/128 bits)) (No client certificate requested) by 7, 47 -- cio-krc-pf03 (Postfix) with ESMTPS id 36C14C0051; Fri, 14 Feb 2014 22:08:54 7, 47 -- -0500 (EST) 7, 47 -- Received: from [192.168.1.102] (75.118.135.65) by smtp.service.osu.edu 7, 47 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.174.1; Fri, 14 Feb 7, 47 -- 2014 22:08:53 -0500 7, 47 -- Message-ID: {52FEDA43.4000002-at-osu.edu} 7, 47 -- Date: Fri, 14 Feb 2014 22:08:51 -0500 7, 47 -- From: Henk Colijn {colijn.1-at-osu.edu} 7, 47 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 7, 47 -- MIME-Version: 1.0 7, 47 -- To: {microscopy-at-microscopy.com} , {jcsmtf-at-mail.missouri.edu} 7, 47 -- Subject: Re: [Microscopy] viaWWW:CM10 Error Message 1 7, 47 -- References: {201402142252.s1EMqq4n007272-at-ns.microscopy.com} 7, 47 -- In-Reply-To: {201402142252.s1EMqq4n007272-at-ns.microscopy.com} 7, 47 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 7, 47 -- Content-Transfer-Encoding: 7bit 7, 47 -- X-Originating-IP: [75.118.135.65] 7, 47 -- X-CFilter-Loop: Forward 7, 47 -- X-FOPE-CRA-Verdict: 164.107.81.210$mail.missouri.edu%0%1%osu.edu%False%False%0$ 7, 47 -- X-OriginatorOrg: osu.edu 7, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 7, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%MAIL.MISSOURI.EDU$RO%1$TLS%0$FQDN%$TlsDn% ==============================End of - Headers==============================
Hi Josh, The ODP needs a heat up time of about 20 min. after the vacuum system has been off for a while. P3 = 0 means it is 100+. When the ODP is heated the vacuum system will continu by opening the valve between the ODP and the camera, then P3 will go down as well. Hans Janssen Netherlands Cancer Institute (NKI/AvL) Amsterdam
_________________________________ Van: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Verzonden: vrijdag 14 februari 2014 23:57 Aan: Hans Janssen Onderwerp: [Microscopy] viaWWW:CM10 HIVAC
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Message: I have not been able to activate the HIVAC and UHV on our CM10. The pressures of the pironi gauges are: P1=82, P2=19, P3=0, IGP=0. This is after the pre-vavc has been running for roughly 20 minutes. Once the pre-vac is turned off P2 goes to about ~70. Is it normal to take longer than this to get the P2 down below 13.3?
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==============================Original Headers============================== 16, 36 -- From j.janssen-at-nki.nl Sat Feb 15 08:18:23 2014 16, 36 -- Received: from out26-ams.mf.surf.net (out26-ams.mf.surf.net [145.0.1.26]) 16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1FEINZP014308 16, 36 -- for {Microscopy-at-Microscopy.com} ; Sat, 15 Feb 2014 08:18:23 -0600 16, 36 -- Received: from edge.nki.nl (edge-1.nki.nl [194.171.7.92]) 16, 36 -- by outgoing1-ams.mf.surf.net (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id s1FEILsf005440 16, 36 -- for {Microscopy-at-Microscopy.com} ; Sat, 15 Feb 2014 15:18:22 +0100 16, 36 -- Received: from caht-1.nki.nl (194.171.7.85) by edge-1.nki.nl (194.171.7.92) 16, 36 -- with Microsoft SMTP Server (TLS) id 8.3.279.1; Sat, 15 Feb 2014 15:18:21 16, 36 -- +0100 16, 36 -- Received: from mstr-1.nki.nl ([172.31.4.101]) by caht-1.nki.nl 16, 36 -- ([172.31.4.103]) with mapi; Sat, 15 Feb 2014 15:18:21 +0100 16, 36 -- From: {j.janssen-at-nki.nl} 16, 36 -- To: {Microscopy-at-Microscopy.com} 16, 36 -- Date: Sat, 15 Feb 2014 15:18:21 +0100 16, 36 -- Subject: RE: [Microscopy] viaWWW:CM10 HIVAC 16, 36 -- Thread-Topic: [Microscopy] viaWWW:CM10 HIVAC 16, 36 -- Thread-Index: Ac8p2DLQS5AiWiArTTSweX7bOOwqqAAf5Vd+ 16, 36 -- Message-ID: {F7BE8A0314FCF948BD9CFA10E54AEABD3261A5A66E-at-mstr-1.nki.nl} 16, 36 -- References: {201402142257.s1EMvqEj015958-at-ns.microscopy.com} 16, 36 -- In-Reply-To: {201402142257.s1EMvqEj015958-at-ns.microscopy.com} 16, 36 -- Accept-Language: nl-NL, en-US 16, 36 -- Content-Language: nl-NL 16, 36 -- X-MS-Has-Attach: 16, 36 -- X-MS-TNEF-Correlator: 16, 36 -- acceptlanguage: nl-NL, en-US 16, 36 -- Content-Type: text/plain; charset="us-ascii" 16, 36 -- MIME-Version: 1.0 16, 36 -- X-Bayes-Prob: 0.0001 (Score 0, tokens from: nki-out:default, base:default, -at--at-RPTN) 16, 36 -- X-Spam-Score: -0.50 () [Tag at 5.00] T_RP_MATCHES_RCVD:-0.01,CC(NL:-0.5) 16, 36 -- X-CanIt-Geo: ip=194.171.7.92; country=NL; region=07; city=Amsterdam; latitude=52.3500; longitude=4.9167; http://maps.google.com/maps?q=52.3500,4.9167&z=6 16, 36 -- X-CanItPRO-Stream: nki-out:default (inherits from nki:default,base:default) 16, 36 -- X-Canit-Stats-ID: 0uLqCilHy - 207119f90c29 - 20140215 (trained as not-spam) 16, 36 -- X-Scanned-By: CanIt (www . roaringpenguin . com) 16, 36 -- Content-Transfer-Encoding: 8bit 16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1FEINZP014308 ==============================End of - Headers==============================
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Email: rrw3q-at-virginia.edu Name: Mr Richard Robert White
Message: We are in need of the plug on a double tilt holder which plugs into a JEOL 2000FX TEM. If you have a broken double tilt holder, we could use the cable and plug from the holder.
Thanks,
Richard White University of Virginia rrw3q-at-virginia.edu
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Sun Feb 16 18:25:41 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1H0PfKU013171 16, 28 -- for {microscopy-at-microscopy.com} ; Sun, 16 Feb 2014 18:25:41 -0600 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id EF8E06BEE6F 16, 28 -- for {microscopy-at-microscopy.com} ; Sun, 16 Feb 2014 18:25:40 -0600 (CST) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id o1fbDy3zrPn2 for {microscopy-at-microscopy.com} ; 16, 28 -- Sun, 16 Feb 2014 18:25:36 -0600 (CST) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 740D86BEE5D 16, 28 -- for {microscopy-at-microscopy.com} ; Sun, 16 Feb 2014 18:25:36 -0600 (CST) 16, 28 -- Message-ID: {530156FF.70805-at-microscopy.com} 16, 28 -- Date: Sun, 16 Feb 2014 18:25:35 -0600 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:2000FX double tilt holder 16, 28 -- References: {201402162336.s1GNaJuE012713-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201402162336.s1GNaJuE012713-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201402162336.s1GNaJuE012713-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The "Pneumatics" error typically means your air pressure from the compresser to the valves is too low. Ours reads ~90 psi normally. Do you have any of the manuals? The appendix in the operator's manuals has the error codes. The ODP not coming on generally means insufficient cooling water, or the cooling water is too hot/too cold. It should be ~20-22 deg C (~70 F, if your Haskris cooler has the usual US temp gauge).
The viewing glass being pushed out means your vent pressure is high. Your column/camera/specimen airlock vent is hooked up to a dry nitrogen tank, yes? That should be at 1-2 psi, no more.
Re: your other email about pressures: I have not been able to activate the HIVAC and UHV on our CM10. The pressures of the pironi gauges are: P1=82, P2=19, P3=0, IGP=0. This is after the pre-vavc has been running for roughly 20 minutes. Once the pre-vac is turned off P2 goes to about ~70. Is it normal to take longer than this to get the P2 down below 13.3?
Our values in normal operation, after pumping overnight: P1 = 37 P2 = 74 P3 = 34 IGP = 14
Your P1 is high, which may mean a leak. If the column was taken apart, the leak is probably in one of those seals, and may not be visible under inspection. Clean. I've never seen a P2 value as low as yours. Seems wrong, likely connected with a leak, but I'd like a service engineer to explain it. P3 reads 0 either because the pressure is too high for it to come on, or the pressure is less than 34 - P3 goes to zero when the pressure drops below 34.
Also the P2 and/or P3 gauges may be malfunctioning. I'd assume a leak to start with. That's the usual bugaboo after taking apart an EM column.
Do you have an ion getter pump on your CM-10? If yes, disconnect it and *don't* run it until you get the rest of the vacuum system working (unplug connector X8, back of the column just below the IGP).
Phil
} Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10 Error Message } } Message: Upon turning on our CM10 for the first time after the install there is a message saying } "Pneumatics". I can't seem to get the ODP to turn on, and twice now the viewing glass has been } pushed out by the compressed air. Does anyone have any ideas on what the problem may be?
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 11, 31 -- From oshel1pe-at-cmich.edu Mon Feb 17 07:24:53 2014 11, 31 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1HDOqrE011385 11, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 07:24:53 -0600 11, 31 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 11, 31 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1HDOeYc015774; 11, 31 -- Mon, 17 Feb 2014 08:24:52 -0500 11, 31 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 11, 31 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 11, 31 -- 14.3.174.1; Mon, 17 Feb 2014 08:24:49 -0500 11, 31 -- Message-ID: {53020DA1.4050704-at-cmich.edu} 11, 31 -- Date: Mon, 17 Feb 2014 08:24:49 -0500 11, 31 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 11, 31 -- Reply-To: {oshel1pe-at-cmich.edu} 11, 31 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 11, 31 -- MIME-Version: 1.0 11, 31 -- To: {microscopylistserver-noreply-at-microscopy.com} , 11, 31 -- micro 11, 31 -- {Microscopy-at-microscopy.com} 11, 31 -- Subject: Re: [Microscopy] viaWWW:CM10 Error Message 1 11, 31 -- References: {201402142311.s1ENBVgx006752-at-ns.microscopy.com} 11, 31 -- In-Reply-To: {201402142311.s1ENBVgx006752-at-ns.microscopy.com} 11, 31 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 11, 31 -- Content-Transfer-Encoding: 7bit 11, 31 -- X-Originating-IP: [141.209.160.122] 11, 31 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 11, 31 -- X-Spam-Score: -0.50 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 11, 31 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 11, 31 -- X-CanItPRO-Stream: default 11, 31 -- X-Canit-Stats-ID: 02LrpoQPq - 3f88cc77f13c - 20140217 11, 31 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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realname - M Raja Email - plspce-at-amararaja.co.in ORGANIZATION - Amararaja Batteries Ltd EDUCATION - Undergraduate College LOCATION - Tirupathi, Andhra pradesh, India SUBJECT_OF_QUESTION - Color metallography-reg QUESTION - how to prepare etchent for lead base alloys and hardware & software requirement and also difficulties / limitations of color metallographic analysis. Actually i need details about benefits of color micrograph. Please revert back in above mail as soon as possible. Thank you in advance for your support.
==============================Original Headers============================== 3, 28 -- From oshel1pe-at-cmich.edu Mon Feb 17 07:38:45 2014 3, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1HDcj61024541 3, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 07:38:45 -0600 3, 28 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 3, 28 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1HDciP3017464 3, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 3, 28 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 3, 28 -- 14.3.174.1; Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- Message-ID: {530210E4.4040306-at-cmich.edu} 3, 28 -- Date: Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 3, 28 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 28 -- MIME-Version: 1.0 3, 28 -- To: micro {Microscopy-at-microscopy.com} 3, 28 -- Subject: Ask-A-Microscopist: etchant for lead alloys & any advantage to color 3, 28 -- imaging in metallography? 3, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 28 -- Content-Transfer-Encoding: 7bit 3, 28 -- X-Originating-IP: [141.209.160.122] 3, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 28 -- X-Spam-Score: 0.00 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,_L_SUPPORT,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 28 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 3, 28 -- X-CanItPRO-Stream: default 3, 28 -- X-Canit-Stats-ID: 02LrpCIWa - 649424478da2 - 20140217 3, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I'd try "Metallogtaphy: Principles and Practices by George F Vander Voot
It's an excellent book.
In some color staining systems, as I understand it, the physical orientation of the metal crystal results in a coloration that is constant for that orientation in that specific sample. Color photomicroscopy lets you image where these orientations are. Color provides contrast that simple gray scale may not.
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Monday, February 17, 2014 8:49 AM To: Frank Karl
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
realname - M Raja Email - plspce-at-amararaja.co.in ORGANIZATION - Amararaja Batteries Ltd EDUCATION - Undergraduate College LOCATION - Tirupathi, Andhra pradesh, India SUBJECT_OF_QUESTION - Color metallography-reg QUESTION - how to prepare etchent for lead base alloys and hardware & software requirement and also difficulties / limitations of color metallographic analysis. Actually i need details about benefits of color micrograph. Please revert back in above mail as soon as possible. Thank you in advance for your support.
==============================Original Headers============================== 3, 28 -- From oshel1pe-at-cmich.edu Mon Feb 17 07:38:45 2014 3, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1HDcj61024541 3, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 07:38:45 -0600 3, 28 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 3, 28 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s1HDciP3017464 3, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 3, 28 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 3, 28 -- 14.3.174.1; Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- Message-ID: {530210E4.4040306-at-cmich.edu} 3, 28 -- Date: Mon, 17 Feb 2014 08:38:44 -0500 3, 28 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 3, 28 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 28 -- MIME-Version: 1.0 3, 28 -- To: micro {Microscopy-at-microscopy.com} 3, 28 -- Subject: Ask-A-Microscopist: etchant for lead alloys & any advantage to color 3, 28 -- imaging in metallography? 3, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 28 -- Content-Transfer-Encoding: 7bit 3, 28 -- X-Originating-IP: [141.209.160.122] 3, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 28 -- X-Spam-Score: 0.00 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,_L_SUPPORT,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 28 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 3, 28 -- X-CanItPRO-Stream: default 3, 28 -- X-Canit-Stats-ID: 02LrpCIWa - 649424478da2 - 20140217 3, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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==============================Original Headers============================== 15, 29 -- From frank_karl-at-ardl.com Mon Feb 17 09:32:27 2014 15, 29 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1HFWQPI018515 15, 29 -- for {microscopy-at-microscopy.com} ; Mon, 17 Feb 2014 09:32:26 -0600 15, 29 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 15, 29 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 17 15, 29 -- Feb 2014 10:32:25 -0500 15, 29 -- From: Frank Karl {frank_karl-at-ardl.com} 15, 29 -- To: "'plspce-at-amararaja.co.in'" {plspce-at-amararaja.co.in} , 15, 29 -- "Microscopy 15, 29 -- Listserver (microscopy-at-microscopy.com)" {microscopy-at-microscopy.com} 15, 29 -- Date: Mon, 17 Feb 2014 10:32:25 -0500 15, 29 -- Subject: RE: [Microscopy] Ask-A-Microscopist: etchant for lead alloys & any 15, 29 -- advantage to color 15, 29 -- Thread-Topic: [Microscopy] Ask-A-Microscopist: etchant for lead alloys & any 15, 29 -- advantage to color 15, 29 -- Thread-Index: Ac8r5yfm+m0+puuoSgu3hRRq6Fv+pgADUywg 15, 29 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BD2D1B12C-at-exchange2k7.ad.ardl.com} 15, 29 -- References: {201402171349.s1HDnA2v011694-at-ns.microscopy.com} 15, 29 -- In-Reply-To: {201402171349.s1HDnA2v011694-at-ns.microscopy.com} 15, 29 -- Accept-Language: en-US 15, 29 -- Content-Language: en-US 15, 29 -- X-MS-Has-Attach: 15, 29 -- X-MS-TNEF-Correlator: 15, 29 -- acceptlanguage: en-US 15, 29 -- Content-Type: text/plain; charset="us-ascii" 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1HFWQPI018515 ==============================End of - Headers==============================
We've just updated our image processing instructions here at the NUANCE Center from Northwestern University.
These instructions are super easy to use and walk the user step-by-(sometimes painful) step to do all sorts of normal image processing procedures one might normally come across, from adjusting levels, to calculations, to some totally cheating techniques of drawing a mag line marker, to several different procedures to apply false color to an image.
THE EXTRA SPECIAL PART is the chapter on what I call Multi-Detector Color. Now I know I did not invent this technique, BUT I've worked out some pretty simple procedures that will allow you to make these really fantastic color images, even if you only have 1 SE detector in your SEM. It's totally free, so please check it out and let us know if you have corrections or suggestions.
I would appreciate hearing suggestions for a good mounting technique for imaging activated carbon and synthetic diamond particles (1-200 micron). When using standard conductive double sided adhesive carbon tabs on stubs, it is obviously quite challenging to get adequate contrast between the particles and the background (carbon tab).
Likewise, in doing particle size analysis it helps to not have touching particles and clumps. Any techniques that have been found to be successful would be interesting to hear.
Thank You!!
Mike Toalson Western Analytical Solutions, LLC o • 208.899.4425 m • 208.340.3895 w • www.materialanalyzers.com e • mike-at-materialanalyzers.com
==============================Original Headers============================== 9, 36 -- From mike-at-materialanalyzers.com Tue Feb 18 14:54:42 2014 9, 36 -- Received: from smtp106.biz.mail.ne1.yahoo.com (smtp106.biz.mail.ne1.yahoo.com [98.138.207.13]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id s1IKsgS8017426 9, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 14:54:42 -0600 9, 36 -- Received: (qmail 26061 invoked from network); 18 Feb 2014 20:54:41 -0000 9, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1392756881; bh=KL2fkLFHhxaw5G1tks+ZRF2GoZjrNk45IrZ0DhKP7Yg=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:From:To:Subject:Date:Organization:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language; b=Xpq18iCUznm1HOZ15Vc9lceTBiNd6C/kY8IUwM7EeTBfedjlacebVr09j9KYUXnFSbY/+Ivpyn5C8AahC/o+bz3ckjZTIIREP5quhyz8rVRxF0yd0Z4+ZNH6B+pPpKTog6gdw0gpPu8Ib/ne8O2KXgTmu14ULhCZJsq5/C6Yviw= 9, 36 -- X-Yahoo-Newman-Property: ymail-3 9, 36 -- X-YMail-OSG: vBrGDPsVM1lG8qIrqCO6h8tM2v7clDoP8_UWbFYJSm.LeU9 9, 36 -- kk8wiiDx3ogannCr3Py1MrVjH9tqIBqPq423NN8iG8a.Bq7.0GfAJkjXMUNl 9, 36 -- gQDBgKGrKXwwkMQZpr_ezoUcX8UAKafNI3A3Z9u56pVh30oMFZiYF8.yEpUX 9, 36 -- KKXkVgJaXFZd3KqDpZPmYbRVI6RZbxpBufFoo6s.Xss8CZIi8VHWqlReUtQs 9, 36 -- 949jqhP9gR.ABKtNdVKP03_Szp0LKjjKTIE60ArvkNuHjfQPclq.PpEqAL.g 9, 36 -- 34p66VV5MbTsNf2uXUmxyt6V7tD.oNhmLpNUAE8IPX8SODVrOqpMGaUIVe9V 9, 36 -- JbkYiVsacekAz16UQQqn5d1c6d9COyUCnMiRGkpZ_lQn71XIhED_R9gYtX0N 9, 36 -- kuIM1C3_uJBvduvlMacERDCKw.5gR90RH_LM7THDqdjzh_hoPSfAN._OHFZ8 9, 36 -- 9ugvnwxGyLZlI5uJMrTSFEeDrznqcWGpaMM2cI3STgat2Gd5xU3y_uTLIDEb 9, 36 -- LBAs4wT4GFVMs2s1Fo_ZDrAI6edHekL5Bq4Ewy6r4r4UmukAEzVUaZBw9jQo 9, 36 -- Zf4Hn8wOTxOTJ_7gNkT5K5fwWkN34nIetrmWuh.c6GGGsAduK8q.ID_dMVDU 9, 36 -- zOZV51jSmMp5PqXp5B2pkgD64gA-- 9, 36 -- X-Yahoo-SMTP: DCD965GswBCJIEnJvHD.h6b811gJzDyc5pf1kxJt 9, 36 -- X-Rocket-Received: from MikeLaptop (mike-at-24.119.142.15 with plain [98.138.105.25]) 9, 36 -- by smtp106.biz.mail.ne1.yahoo.com with SMTP; 18 Feb 2014 12:54:41 -0800 PST 9, 36 -- From: "Mike Toalson" {mike-at-materialanalyzers.com} 9, 36 -- To: {Microscopy-at-microscopy.com} 9, 36 -- Subject: SEM - Suggested mounting method for Carbon particles 9, 36 -- Date: Tue, 18 Feb 2014 13:54:30 -0700 9, 36 -- Organization: Western Analytical Solutions, LLC 9, 36 -- Message-ID: {027501cf2ceb$9f16ec10$dd44c430$-at-materialanalyzers.com} 9, 36 -- MIME-Version: 1.0 9, 36 -- Content-Type: text/plain; 9, 36 -- charset="iso-8859-1" 9, 36 -- X-Mailer: Microsoft Outlook 14.0 9, 36 -- Thread-Index: Ac8s6iyYCvomrZCeSeSlOzEyRqtHcw== 9, 36 -- Content-Language: en-us 9, 36 -- Content-Transfer-Encoding: 8bit 9, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IKsgS8017426 ==============================End of - Headers==============================
Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images.
Pete Eschbach Oregon State University
Sent from my iPhone
==============================Original Headers============================== 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 3, 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 3, 32 -- Subject: Montage software? 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 3, 32 -- Content-Type: text/plain; 3, 32 -- charset=us-ascii 3, 32 -- X-Mailer: iPhone Mail (11B554a) 3, 32 -- Message-Id: {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 3, 32 -- To: Microscopy-at-microscopy.com 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 3, 32 -- s=q20121106; t=1392761924; 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: 3, 32 -- Mime-Version; 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC 3, 32 -- vXeoTIY3Fw3YA== 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IMIi4s005720 ==============================End of - Headers==============================
The ImageJ plug-in MosaicJ is pretty easy to use and allows big images. I believe you need the TurboReg plug-in installed as well, as it can automatically stitch as long as you put them down close to the right position. I've used it successfully several times. Good Luck! Larry Scipioni ZS Genetics
-----Original Message----- X-from: peter.eschbach-at-comcast.net [mailto:peter.eschbach-at-comcast.net] Sent: Tuesday, February 18, 2014 5:30 PM To: LES-at-ZSGENETICS.COM
Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images.
Pete Eschbach Oregon State University
Sent from my iPhone
==============================Original Headers============================== 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 3, 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 3, 32 -- Subject: Montage software? 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 3, 32 -- Content-Type: text/plain; 3, 32 -- charset=us-ascii 3, 32 -- X-Mailer: iPhone Mail (11B554a) 3, 32 -- Message-Id: {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 3, 32 -- To: Microscopy-at-microscopy.com 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 3, 32 -- s=q20121106; t=1392761924; 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: 3, 32 -- Mime-Version; 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC 3, 32 -- vXeoTIY3Fw3YA== 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IMIi4s005720 ==============================End of - Headers==============================
==============================Original Headers============================== 10, 44 -- From les-at-zsgenetics.com Tue Feb 18 17:41:03 2014 10, 44 -- Received: from gateway01.websitewelcome.com ([74.52.222.242]) 10, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1INf3IB012377 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 17:41:03 -0600 10, 44 -- Received: by gateway01.websitewelcome.com (Postfix, from userid 5007) 10, 44 -- id D0AF25B3B6E87; Tue, 18 Feb 2014 17:41:02 -0600 (CST) 10, 44 -- Received: from gator2013.hostgator.com (gator2013.hostgator.com [50.87.144.13]) 10, 44 -- by gateway01.websitewelcome.com (Postfix) with ESMTP id 9D45D5B3B6DBB 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 17:41:02 -0600 (CST) 10, 44 -- Received: from [146.115.5.114] (port=63647 helo=LESZSG) 10, 44 -- by gator2013.hostgator.com with esmtpsa (TLSv1:AES128-SHA:128) 10, 44 -- (Exim 4.80) 10, 44 -- (envelope-from {les-at-zsgenetics.com} ) 10, 44 -- id 1WFuHe-00006Z-3V; Tue, 18 Feb 2014 17:41:02 -0600 10, 44 -- From: "Larry Scipioni" {les-at-zsgenetics.com} 10, 44 -- To: {peter.eschbach-at-comcast.net} 10, 44 -- Cc: {microscopy-at-microscopy.com} 10, 44 -- References: {201402182230.s1IMUCmo022411-at-ns.microscopy.com} 10, 44 -- In-Reply-To: {201402182230.s1IMUCmo022411-at-ns.microscopy.com} 10, 44 -- Subject: RE: [Microscopy] Montage software? 10, 44 -- Date: Tue, 18 Feb 2014 18:41:04 -0500 10, 44 -- Organization: ZS Genetics 10, 44 -- Message-ID: {00d201cf2d02$e407e1a0$ac17a4e0$-at-zsgenetics.com} 10, 44 -- MIME-Version: 1.0 10, 44 -- Content-Type: text/plain; 10, 44 -- charset="us-ascii" 10, 44 -- Content-Transfer-Encoding: 7bit 10, 44 -- X-Mailer: Microsoft Outlook 14.0 10, 44 -- Thread-Index: AQJwEA55ksWYCeFAf44NBX/BI5A8nJl55O1Q 10, 44 -- Content-Language: en-us 10, 44 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 10, 44 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com 10, 44 -- X-AntiAbuse: Original Domain - microscopy.com 10, 44 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 10, 44 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com 10, 44 -- X-BWhitelist: no 10, 44 -- X-Source-IP: 146.115.5.114 10, 44 -- X-Source: 10, 44 -- X-Source-Args: 10, 44 -- X-Source-Dir: 10, 44 -- X-Source-Sender: (LESZSG) [146.115.5.114]:63647 10, 44 -- X-Source-Auth: les-at-zsgenetics.com 10, 44 -- X-Email-Count: 2 10, 44 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20= ==============================End of - Headers==============================
I've used the GIMP for this application with a lot of success. Something else to look at would be this: http://research.microsoft.com/en-us/um/redmond/groups/ivm/ICE/
-Jacob
On 14-02-18 02:29 PM, peter.eschbach-at-comcast.net wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images. } } Pete Eschbach } Oregon State University } } Sent from my iPhone } } ==============================Original Headers============================== } 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 } 3, 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) } 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 } 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 } 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) } 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast } 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 } 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) } 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast } 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 } 3, 32 -- Subject: Montage software? } 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} } 3, 32 -- Content-Type: text/plain; } 3, 32 -- charset=us-ascii } 3, 32 -- X-Mailer: iPhone Mail (11B554a) } 3, 32 -- Message-Id: {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} } 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 } 3, 32 -- To: Microscopy-at-microscopy.com } 3, 32 -- Mime-Version: 1.0 (1.0) } 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; } 3, 32 -- s=q20121106; t=1392761924; } 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; } 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: } 3, 32 -- Mime-Version; } 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 } 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 } 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y } 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m } 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC } 3, 32 -- vXeoTIY3Fw3YA== } 3, 32 -- Content-Transfer-Encoding: 8bit } 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IMIi4s005720 } ==============================End of - Headers============================== } }
==============================Original Headers============================== 3, 28 -- From jkabel-at-mail.ubc.ca Tue Feb 18 18:33:21 2014 3, 28 -- Received: from vmaprod4.mail-relay.ubc.ca (vmaprod4.mail-relay.ubc.ca [142.103.117.138]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1J0XLbt031713 3, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 18:33:21 -0600 3, 28 -- Received: from vmaprod4.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 3, 28 -- by localhost (Email Security Appliance) with SMTP id D179326B941_303FBD0B 3, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 00:33:20 +0000 (GMT) 3, 28 -- Received: from mx2.mail-relay.ubc.ca (lb_mx-out.mail-relay.ubc.ca [10.92.8.24]) 3, 28 -- by vmaprod4.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTP id 9981826BA7D_303FBD0F 3, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 00:33:20 +0000 (GMT) 3, 28 -- Received: from smtp.mail.ubc.ca (s-itsv-hub04p.ead.ubc.ca [137.82.151.86]) 3, 28 -- by mx2.mail-relay.ubc.ca (Postfix) with ESMTP id 9153615F66A 3, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:33:20 -0800 (PST) 3, 28 -- Received: from [137.82.16.57] (137.82.16.57) by smtp.mail.ubc.ca 3, 28 -- (137.82.151.86) with Microsoft SMTP Server id 14.3.174.1; Tue, 18 Feb 2014 3, 28 -- 16:33:20 -0800 3, 28 -- Message-ID: {5303FBD0.2000509-at-mail.ubc.ca} 3, 28 -- Date: Tue, 18 Feb 2014 16:33:20 -0800 3, 28 -- From: Jacob Kabel {jkabel-at-mail.ubc.ca} 3, 28 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 3, 28 -- MIME-Version: 1.0 3, 28 -- To: {Microscopy-at-microscopy.com} 3, 28 -- Subject: Re: [Microscopy] Montage software? 3, 28 -- References: {201402182229.s1IMTlmO021444-at-ns.microscopy.com} 3, 28 -- In-Reply-To: {201402182229.s1IMTlmO021444-at-ns.microscopy.com} 3, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 28 -- Content-Transfer-Encoding: 7bit 3, 28 -- X-Originating-IP: [137.82.16.57] ==============================End of - Headers==============================
I have used the commercial program Corel Photopaint (part of the Coreldraw suite of programs) extensively over the years for TEM and other montaging. It is manual montaging, but it handles any file size (I have done up to montage around 300 GB), and it has a feature where the selected image is subtracted from what is under it, meaning you can quickly see which parts of the image are aligned because they go completely black.
The programs others have mentioned are also good options.
Kind regards Duane Harland AgResearch New Zealand
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Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images.
Pete Eschbach Oregon State University
Sent from my iPhone
==============================Original Headers============================== 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 3, 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 3, 32 -- Subject: Montage software? 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 3, 32 -- Content-Type: text/plain; 3, 32 -- charset=us-ascii 3, 32 -- X-Mailer: iPhone Mail (11B554a) 3, 32 -- Message-Id: {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 3, 32 -- To: Microscopy-at-microscopy.com 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 3, 32 -- s=q20121106; t=1392761924; 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: 3, 32 -- Mime-Version; 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC 3, 32 -- vXeoTIY3Fw3YA== 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1IMIi4s005720 ==============================End of - Headers==============================
"Metallographic etchnig" by Gűnter Petzow edited by ASM 1999, black white photo lead structure was published in the practicall metalography many years ago (1970 ?)
best regards
best regards
Chris Hűbner
Cracow University of Technology Faculty of Mechanical Engineering
----- Original Message ----- X-from: {oshel1pe-at-cmich.edu} To: {hubner-at-mech.pk.edu.pl} Sent: Monday, February 17, 2014 2:50 PM
Dear Microscopists,
maybe someone can give me a hint on how to proceed now. We just encountered a problem with our Tecnai Osiris in STEM mode: We reduced the acceleration voltage from 200kV to 80kV to deal with a little bit sensitive samples. In TEM Mode we didn´t had any problems, but in STEM we see a distortion. I uploaded a picture* of the problem. We suspect some magnetic field could be responsible for this behavior.
What do you think? And what could we do to find the reason?
I start with double sticky conductive tape on a stub. I spread the powder out on a glass slide and blow the powder off the slide and onto the stub. Just a puff, a little breath, a mouse fart. A small mouse.
You can position the stub below or across from your slide. Too much powder on stub? Blow it off, use a bigger mouse this time.
What you want is a single layer of crystals/particles, separated as much as possible from each other. The stub will look sample deficient to the eye, check it with a stereomicroscope and I suspect you'll find plenty.
I coat these samples with Au or Pt, twice. One coating straight on and then I lay the SEM mount in the chamber at an angle to get the "underside" of the particles. Works great. I've been running organic accelerators for rubber this way for years and have always been happy with results.
Good Luck!
Frank
-----Original Message----- X-from: mike-at-materialanalyzers.com [mailto:mike-at-materialanalyzers.com] Sent: Tuesday, February 18, 2014 4:03 PM To: Frank Karl
I would appreciate hearing suggestions for a good mounting technique for imaging activated carbon and synthetic diamond particles (1-200 micron). When using standard conductive double sided adhesive carbon tabs on stubs, it is obviously quite challenging to get adequate contrast between the particles and the background (carbon tab).
Likewise, in doing particle size analysis it helps to not have touching particles and clumps. Any techniques that have been found to be successful would be interesting to hear.
Thank You!!
Mike Toalson Western Analytical Solutions, LLC o * 208.899.4425 m * 208.340.3895 w * www.materialanalyzers.com e * mike-at-materialanalyzers.com
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==============================Original Headers============================== 27, 29 -- From frank_karl-at-ardl.com Wed Feb 19 09:26:37 2014 27, 29 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 27, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JFQaP8023143 27, 29 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 09:26:36 -0600 27, 29 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 27, 29 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Wed, 19 27, 29 -- Feb 2014 10:26:35 -0500 27, 29 -- From: Frank Karl {frank_karl-at-ardl.com} 27, 29 -- To: "'mike-at-materialanalyzers.com'" {mike-at-materialanalyzers.com} 27, 29 -- CC: "Microscopy Listserver (microscopy-at-microscopy.com)" 27, 29 -- {microscopy-at-microscopy.com} 27, 29 -- Date: Wed, 19 Feb 2014 10:26:34 -0500 27, 29 -- Subject: RE: [Microscopy] SEM - Suggested mounting method for Carbon 27, 29 -- particles 27, 29 -- Thread-Topic: [Microscopy] SEM - Suggested mounting method for Carbon 27, 29 -- particles 27, 29 -- Thread-Index: Ac8s7OEK3Dkn4B9+TbWCOUe2HKie+gAmFesg 27, 29 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BD2D1B130-at-exchange2k7.ad.ardl.com} 27, 29 -- References: {201402182102.s1IL2aHo031064-at-ns.microscopy.com} 27, 29 -- In-Reply-To: {201402182102.s1IL2aHo031064-at-ns.microscopy.com} 27, 29 -- Accept-Language: en-US 27, 29 -- Content-Language: en-US 27, 29 -- X-MS-Has-Attach: 27, 29 -- X-MS-TNEF-Correlator: 27, 29 -- acceptlanguage: en-US 27, 29 -- Content-Type: text/plain; charset="iso-8859-1" 27, 29 -- MIME-Version: 1.0 27, 29 -- Content-Transfer-Encoding: 8bit 27, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1JFQaP8023143 ==============================End of - Headers==============================
3. For coloring lead phases try the Copper Alloy etch set out by:
Beraha: Color Metallography. ASM 1977.
4. Coloring can be an advantage if it adds to the contrast desired to detect or demonstrate a particular phase. This will depend on the material at hand.
5. M Raja, please report back how it turns out.
Tony
…………………………………………………………………………………………………………… Andrew Anthony “Tony” Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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-----Original Message----- X-from: hubner-at-mech.pk.edu.pl [mailto:hubner-at-mech.pk.edu.pl] Sent: Wednesday, February 19, 2014 7:19 AM To: ph2-at-sprynet.com
Hello,
lead etching you found in the book:
"Metallographic etchnig" by Gűnter Petzow edited by ASM 1999, black white photo lead structure was published in the practicall metalography many years ago (1970 ?)
best regards
best regards
Chris Hűbner
Cracow University of Technology Faculty of Mechanical Engineering
----- Original Message ----- X-from: {oshel1pe-at-cmich.edu} To: {hubner-at-mech.pk.edu.pl} Sent: Monday, February 17, 2014 2:50 PM
Hi
Beckert - Klemm "Handbuch der metallographischen Ätzverfahren" VEB Deutsch. Verlag f. Grundstoffindustrie - 1962
Sorry, it's in german, but very usefull. Always on my shelf !
There are 10 pages with different solutions vor lead and lead alloys, with 2 BW pictures and an (old now) bibliography.
Hope it helps
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
} Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images.
a number of options to stitch existing images has been discussed already - if you are interested in software that drives automated acquisition to build montages, have a look at SerialEM (actually a tomography software):
http://bio3d.colorado.edu/SerialEM/index.html
It works on the Titan platform and can also do the stitching.
Best,
Guenter
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
==============================Original Headers============================== 8, 19 -- From lists-at-nexperion.net Wed Feb 19 11:33:43 2014 8, 19 -- Received: from sh-503.premium.hosttech.eu (sh-503.premium.hosttech.eu [46.4.82.76]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JHXhvh017355 8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 11:33:43 -0600 8, 19 -- X-No-Relay: not in my network 8, 19 -- Received: from aventine (chello080108026214.8.11.vie.surfer.at [80.108.26.214]) 8, 19 -- by sh-503.premium.hosttech.eu (Postfix) with ESMTPSA id CB6BA11F502E 8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 18:33:41 +0100 (CET) 8, 19 -- Date: Wed, 19 Feb 2014 18:33:39 +0100 8, 19 -- From: Guenter Resch {lists-at-nexperion.net} 8, 19 -- To: Microscopy-at-microscopy.com 8, 19 -- Subject: Re: [Microscopy] Montage software? 8, 19 -- Message-ID: {20140219183339.1b468998-at-aventine} 8, 19 -- In-Reply-To: {201402182230.s1IMUhOr023549-at-ns.microscopy.com} 8, 19 -- References: {201402182230.s1IMUhOr023549-at-ns.microscopy.com} 8, 19 -- X-Mailer: Claws Mail 3.8.1 (GTK+ 2.24.20; x86_64-pc-linux-gnu) 8, 19 -- Mime-Version: 1.0 8, 19 -- Content-Type: text/plain; charset=US-ASCII 8, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Montage building software you use will be dependent not so much on the microscope but how the images are named and in what order they are collected. If you are using a FEI Eagle camera, you will need to convert the proprietary image format to .tif or .jpg then proceed. GIMP does this well. We use an AMT camera and the images are automatically named according to their x-y position in the montage, for example 0006R1C6 refers to an image in the first row and the sixth column. How the stage moves from one image to the next during collection is essential information. We have used AdobePhotoshop for image montages, but it is slow compared to FIJI and may not complete large montages. For montages up to 19x19 images, I would suggest using FIJI. For image sets of this size you will also want a 64 bit operating system with exceptional graphics capabilities. Download Fiji (http://fiji.sc/wiki/index.php/Downloads). Fiji is a distribution of ImageJ together with Java, Java 3D and several plugins organized to assist research in life sciences, targeting image registration, stitching, segmentation, feature extraction and 3D visualization, among others. Using FIJI you can direct the program to the folder in which the images are stored and also define the naming nomenclature so that the montage software will find each successive image. There is quite a bit of flexibility built into FIJI as to how the program finds the images; we have had the most success with the position defined by the file name. Once you download FIJI, bring up the screen where images in this format can be loaded (FIJI/Stitching/Deprecated/Stitch grid of images). I would be happy to send a word document with specific instructions with screen shots on how to work with images in this format to anyone who might find it useful.
Cheers,
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3101 SW Sam Jackson Park Road Portland, Oregon 97239 Office: 503-221-3434 Mobile: 503-819-3600
} Does anybody know of software for building Montage images for a Titan TEM? We would like to build a large image from a multitude of high resolution plant cell images. } } Pete Eschbach } Oregon State University } } Sent from my iPhone } } ==============================Original } Headers============================== } 3, 32 -- From peter.eschbach-at-comcast.net Tue Feb 18 16:18:45 2014 3, } 32 -- Received: from qmta05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) } 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1IMIi4s005720 } 3, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2014 16:18:45 -0600 } 3, 32 -- Received: from omta07.emeryville.ca.mail.comcast.net ([76.96.30.59]) } 3, 32 -- by qmta05.emeryville.ca.mail.comcast.net with comcast } 3, 32 -- id TrWT1n0061GXsucA5yJkN9; Tue, 18 Feb 2014 22:18:44 +0000 } 3, 32 -- Received: from [10.0.4.46] ([207.55.8.2]) } 3, 32 -- by omta07.emeryville.ca.mail.comcast.net with comcast } 3, 32 -- id TyJP1n00702dqGn8UyJUxb; Tue, 18 Feb 2014 22:18:39 +0000 } 3, 32 -- Subject: Montage software? } 3, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 3, 32 -- } Content-Type: text/plain; } 3, 32 -- charset=us-ascii } 3, 32 -- X-Mailer: iPhone Mail (11B554a) 3, 32 -- Message-Id: } {A8B20AE2-CC8B-4CA7-BD7D-25C7215666A2-at-comcast.net} } 3, 32 -- Date: Tue, 18 Feb 2014 14:18:24 -0800 3, 32 -- To: } Microscopy-at-microscopy.com 3, 32 -- Mime-Version: 1.0 (1.0) 3, 32 -- } DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; } 3, 32 -- s=q20121106; t=1392761924; } 3, 32 -- bh=dCiLtwod0fmaajKTaaCKcw/ogeCSgpHD2sWDpix8fTQ=; } 3, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: } 3, 32 -- Mime-Version; } 3, 32 -- b=ki/C1vTG0vFQ7D/4snReM5xUQSVSaC/vW0xhn9VIkRXOXBOHIcH59njCFfvOaqeK3 } 3, 32 -- AOsFMrjgqUQQl3MIe7Wibyf+RHzRFemtsycDZSZ26LciEHXPFiRGFDD6smh9PSqOH5 } 3, 32 -- EisISsoA5uuT2NU3DSMza9HbGGb+FzMj24TBsJJIINs5EsTISaP+n7RUwLADCNcE+Y } 3, 32 -- Zkp3z+qoR0+Gsxr705uSFY/EYZSe2YkHELmDDSrfTeG1cJOYCghTbzUwsi03uCz35m } 3, 32 -- dHrj4zXiwmRRHDqzpC+oH65uGZakULyRW2bkwBYCRvc0IHOjC1B6IQSNAY5hUf9VVC } 3, 32 -- vXeoTIY3Fw3YA== } 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s1IMIi4s005720 ==============================End } of - Headers============================== } }
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==============================Original Headers============================== 6, 36 -- From DRK-at-shcc.org Wed Feb 19 11:56:28 2014 6, 36 -- Received: from mail1.bemta8.messagelabs.com (mail1.bemta8.messagelabs.com [216.82.243.203]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JHuRA6004094 6, 36 -- for {Microscopy-at-Microscopy.Com} ; Wed, 19 Feb 2014 11:56:28 -0600 6, 36 -- Received: from [216.82.242.115:2097] by server-11.bemta-8.messagelabs.com id 16/E0-30795-B40F4035; Wed, 19 Feb 2014 17:56:27 +0000 6, 36 -- X-Env-Sender: DRK-at-shcc.org 6, 36 -- X-Msg-Ref: server-13.tower-132.messagelabs.com!1392832494!12679344!105 6, 36 -- X-Originating-IP: [216.166.12.178] 6, 36 -- X-StarScan-Received: 6, 36 -- X-StarScan-Version: 6.9.16; banners=-,-,- 6, 36 -- X-VirusChecked: Checked 6, 36 -- Received: (qmail 2235 invoked from network); 19 Feb 2014 17:56:27 -0000 6, 36 -- Received: from out001.collaborationhost.net (HELO out001.collaborationhost.net) (216.166.12.178) 6, 36 -- by server-13.tower-132.messagelabs.com with RC4-SHA encrypted SMTP; 19 Feb 2014 17:56:27 -0000 6, 36 -- Received: from AUSP01VMBX30.collaborationhost.net ([10.2.12.44]) by 6, 36 -- AUSP01MHUB04.collaborationhost.net ([10.2.0.189]) with mapi; Wed, 19 Feb 2014 6, 36 -- 11:57:15 -0600 6, 36 -- From: Doug Keene {DRK-at-shcc.org} 6, 36 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 6, 36 -- Date: Wed, 19 Feb 2014 11:56:21 -0600 6, 36 -- Subject: RE: [Microscopy] Re: Montage software? 6, 36 -- Thread-Topic: [Microscopy] Re: Montage software? 6, 36 -- Thread-Index: Ac8tC2iZmfHZnAiGS2mQq5ze4h96RAAjKW7g 6, 36 -- Message-ID: {1DDBEFFEDA3EED4B8817469E593164AA6BC94881DA-at-AUSP01VMBX30.collaborationhost.net} 6, 36 -- References: {201402190041.s1J0f6sk011088-at-ns.microscopy.com} 6, 36 -- In-Reply-To: {201402190041.s1J0f6sk011088-at-ns.microscopy.com} 6, 36 -- Accept-Language: en-US 6, 36 -- Content-Language: en-US 6, 36 -- X-MS-Has-Attach: 6, 36 -- X-MS-TNEF-Correlator: 6, 36 -- x-vipre-scanned: 25769B1B00680C25769C68 6, 36 -- acceptlanguage: en-US 6, 36 -- Content-Type: text/plain; charset="us-ascii" 6, 36 -- MIME-Version: 1.0 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1JHuRA6004094 ==============================End of - Headers==============================
There are many montaging programs. I can suggest PTGUI which some of our SEM users have used for montages with hundreds of images. It is not picky on the filenames, filetypes, and/or file order.
PTGUI stands for Panorama Tools (Graphic User Interface).
regards,
- Jim
==============================Original Headers============================== 5, 26 -- From jquinn11733-at-gmail.com Wed Feb 19 14:46:21 2014 5, 26 -- Received: from mail-la0-f51.google.com (mail-la0-f51.google.com [209.85.215.51]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JKkKcq026122 5, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 14:46:21 -0600 5, 26 -- Received: by mail-la0-f51.google.com with SMTP id c6so688718lan.24 5, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 12:46:20 -0800 (PST) 5, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 26 -- d=gmail.com; s=20120113; 5, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 5, 26 -- bh=nY7stHr3bJct1VSuuYydCgMXvAzlDnrv1yJfpTVQ7As=; 5, 26 -- b=Ej4EM/ezeoHDTdgxKuh/tldikL1G8PkboGaY0jb1PtDJNjse9MFC6RtQ/18V2eKUm8 5, 26 -- ERgimw7tN0j7BduVU3WF77OMZpb5IEK90TsUfBazdURebUeWqOUvQlecwCxPkfeyl4of 5, 26 -- iUfZPjfaXNzig/cI4u5pzAlZJJxxQUn5HhyxVdD03ZwEQMBFxfFD/yxfo5zO3BbANSlO 5, 26 -- cnGpDY8B5+Em8P02zOSCeK1z6vjusQsjIpgfEX/Glvb3J8rHj/3UFSsj3+Vyf3aOxkPy 5, 26 -- j+9PWupEmY/xYbYYCg9rzg46TchTQF20ikwOUK/EpgSAqaqkewZcF1I9wc4dcZRIOE6e 5, 26 -- 3Tdw== 5, 26 -- MIME-Version: 1.0 5, 26 -- X-Received: by 10.112.56.237 with SMTP id d13mr26179360lbq.2.1392842780112; 5, 26 -- Wed, 19 Feb 2014 12:46:20 -0800 (PST) 5, 26 -- Received: by 10.112.218.3 with HTTP; Wed, 19 Feb 2014 12:46:20 -0800 (PST) 5, 26 -- Date: Wed, 19 Feb 2014 15:46:20 -0500 5, 26 -- Message-ID: {CAO_SM8KtqaPa4Aht6vAwQNiqSOUmd+Q6ke8nKZorTCZ=-ezbdw-at-mail.gmail.com} 5, 26 -- Subject: montage with PTGUI 5, 26 -- From: Jim Quinn {jquinn11733-at-gmail.com} 5, 26 -- To: Microscopy-at-microscopy.com 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
I did not send any of you a message in the last two days. Please do not open if you did receive one for I do not know where the message originated. -- Pat Patricia Stranen Connelly NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491
==============================Original Headers============================== 3, 58 -- From connellyps-at-nhlbi.nih.gov Wed Feb 19 16:31:06 2014 3, 58 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 3, 58 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JMV6wx014141 3, 58 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 16:31:06 -0600 3, 58 -- X-IronPortListener: Outbound_SMTP 3, 58 -- X-IronPort-Anti-Spam-Filtered: true 3, 58 -- X-IronPort-Anti-Spam-Result: AgEFAOYvBVOcKEel/2dsb2JhbABWAw6CeIEPvQGEHRZ0giwSFxFRAQITKS8TJwQBNIdjol6rcReOAhEBaIQnBI5kiUyGb4s1gm4/gXE5 3, 58 -- Received: from unknown (HELO CASHTV01.nih.gov) ([156.40.71.165]) 3, 58 -- by nihxway3out.hub.nih.gov with ESMTP/TLS/AES128-SHA; 19 Feb 2014 17:31:05 -0500 3, 58 -- Received: from MLBXV14.nih.gov ([169.254.4.167]) by CASHTV01.nih.gov 3, 58 -- ([156.40.71.165]) with mapi id 14.03.0174.001; Wed, 19 Feb 2014 17:31:01 3, 58 -- -0500 3, 58 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 3, 58 -- To: ChuckCharles A Garber {cgarber-at-2spi.com} , 3, 58 -- DanDan Connelly 3, 58 -- {dansconnelly-at-hotmail.com} , 3, 58 -- "Sherman, Debra" {dsherman-at-purdue.edu} , 3, 58 -- "DeeDee 3, 58 -- Breger" {deebre-at-coe.drexel.edu} , 3, 58 -- DottyDorothy Sorenson {dsoren-at-umich.edu} , 3, 58 -- GregGreg Guild {gguild-at-sas.upenn.edu} , 3, 58 -- JoelJoel Sheffield {jbs-at-temple.edu} , 3, 58 -- LouiseLouise Harner {louise_harner-at-albint.com} , 3, 58 -- mePat Connelly 3, 58 -- {stranen_connelly-at-yahoo.com} , 3, 58 -- MSA {microscopy-at-microscopy.com} , 3, 58 -- "NoelNoel 3, 58 -- Stranen" {noeltimbo-at-aol.com} , 3, 58 -- SheilaSheila Connelly 3, 58 -- {sheila.connelly-at-avtxinc.com} , 3, 58 -- suekiShoji Ueki {sueki-at-wharton.upenn.edu} , 3, 58 -- TatyanaTatyana Svitkina {svitkina-at-sas.upenn.edu} , 3, 58 -- "Connelly, Patricia 3, 58 -- (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} , 3, 58 -- Bernadette Lawrence 3, 58 -- {bmcl80-at-comcast.net} , 3, 58 -- borders {borders-at-e.bordersstores.com} , 3, 58 -- Dave Heminghaus 3, 58 -- {dheminghaus-at-semtechsolutions.com} , 3, 58 -- David L Jones {dljones-at-bestweb.net} , David Roos {droos-at-sas.upenn.edu} 3, 58 -- Subject: URGENT Do not open earlier email from me 3, 58 -- Thread-Topic: URGENT Do not open earlier email from me 3, 58 -- Thread-Index: AQHPLcJGL9dWjyyUyEePDnY0awitNw== 3, 58 -- Importance: high 3, 58 -- X-Priority: 1 3, 58 -- Date: Wed, 19 Feb 2014 22:31:04 +0000 3, 58 -- Message-ID: {CF2A9AD5.17C2%connellyps-at-mail.nih.gov} 3, 58 -- Accept-Language: en-US 3, 58 -- Content-Language: en-US 3, 58 -- X-MS-Has-Attach: 3, 58 -- X-MS-TNEF-Correlator: 3, 58 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 3, 58 -- x-originating-ip: [156.40.71.188] 3, 58 -- Content-Type: text/plain; charset="us-ascii" 3, 58 -- Content-ID: {36A16DC804ABD146BA1BBD33A896DF44-at-mail.nih.gov} 3, 58 -- MIME-Version: 1.0 3, 58 -- Content-Transfer-Encoding: 8bit 3, 58 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1JMV6wx014141 ==============================End of - Headers==============================
My mail has been hacked or whatever you you call it. I am attempting to find which email was broken into but this was the fastest way to get to a lot of you. I have not sent any messages to the Listerv earlier today.
-- Pat Patricia Stranen Connelly NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491
==============================Original Headers============================== 5, 30 -- From connellyps-at-nhlbi.nih.gov Wed Feb 19 16:42:44 2014 5, 30 -- Received: from nihxway4out.hub.nih.gov (nihxway4out.hub.nih.gov [128.231.90.112]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JMgh2C027234 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 16:42:43 -0600 5, 30 -- X-IronPortListener: Outbound_SMTP 5, 30 -- X-IronPort-Anti-Spam-Filtered: true 5, 30 -- X-IronPort-Anti-Spam-Result: AgEFAMcyBVOcKEeo/2dsb2JhbABWA4MGgQ+9AYQdFnSCLBIoUQEVKS8TJwQ1h2OdaoR2q3EXjgIQAgFnhCcEjmSJTIZvizWDLYIq 5, 30 -- Received: from unknown (HELO CASHTV04.nih.gov) ([156.40.71.168]) 5, 30 -- by nihxway4out.hub.nih.gov with ESMTP/TLS/AES128-SHA; 19 Feb 2014 17:42:43 -0500 5, 30 -- Received: from MLBXV14.nih.gov ([169.254.4.167]) by CASHTV04.nih.gov 5, 30 -- ([156.40.71.168]) with mapi id 14.03.0174.001; Wed, 19 Feb 2014 17:42:43 5, 30 -- -0500 5, 30 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 5, 30 -- To: MSA {microscopy-at-microscopy.com} 5, 30 -- Subject: URGENT do NOT open any mail from Pat Connelly today 5, 30 -- Thread-Topic: URGENT do NOT open any mail from Pat Connelly today 5, 30 -- Thread-Index: AQHPLcPm1AJBqxCyf0WpHAg2ovBQWA== 5, 30 -- Date: Wed, 19 Feb 2014 22:42:42 +0000 5, 30 -- Message-ID: {CF2A9D91.17E5%connellyps-at-mail.nih.gov} 5, 30 -- Accept-Language: en-US 5, 30 -- Content-Language: en-US 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 5, 30 -- x-originating-ip: [156.40.71.192] 5, 30 -- Content-Type: text/plain; charset="us-ascii" 5, 30 -- Content-ID: {AA896E870F9CF3428DB766ED7EBFD453-at-mail.nih.gov} 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1JMgh2C027234 ==============================End of - Headers==============================
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Email: alpinto-at-fm.ul.pt Name: Andreia Pinto
Organization: Instituto de Medicina Molecular
Title-Subject: [Filtered] JEOL JEM-100CX II diaphragms
Message: We have here at the Institute an old Jeol JEM-100CX II that was disconnected for many years, now it is finally working again, but, it needs new diaphragms, so, we are searching for good options in acquiring/buying diaphragms for this brand/model, can you help me?? We really can't spend a lot of money, so, we are also prone to accept used diaphragms, if they are in good shape.
Thank you very much!!
Andreia L. Pinto Comparative Pathology Laboratory Instituto Medicina Molecular Lisbon - Portugal
e-mail: alpinto-at-fm.ul.pt
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 19 17:08:09 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1JN89gY027659 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 17:08:09 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 3E6C26C1330 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 17:08:09 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id bsGzJl+6YdlL for {microscopy-at-microscopy.com} ; 19, 28 -- Wed, 19 Feb 2014 17:08:02 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 59F776C1323 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 17:08:02 -0600 (CST) 19, 28 -- Message-ID: {53053952.7060500-at-microscopy.com} 19, 28 -- Date: Wed, 19 Feb 2014 17:08:02 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:JEOL JEM-100CX II diaphragms 19, 28 -- References: {201402191359.s1JDx5tI002583-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201402191359.s1JDx5tI002583-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201402191359.s1JDx5tI002583-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Max, You're probably seeing the effect of a stray AC field on your scan. A paper by Muller et al. from Ultramicroscopy describes ways to design a room to minimize this kind of distortion: http://www.sciencedirect.com/science/article/pii/S0304399106001033
Do you notice the distortion change when you change your scan rate? Right now, it appears that the period of the field's oscillation is equal to the time necessary to complete a couple of scan lines. If you can find a scan rate at which the distortion period is equal to the time necessary to complete an integer number of scan lines, you should be able to calculate the frequency of the distortion (I bet it's 60 Hz!) which may help you locate the source of the field.
Tyler
On 2014-02-19 6:50, raven2020-at-gmx.de wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Microscopists, } } maybe someone can give me a hint on how to proceed now. } We just encountered a problem with our Tecnai Osiris in } STEM mode: } We reduced the acceleration voltage from 200kV to 80kV } to deal with a little bit sensitive samples. In TEM Mode } we didn´t had any problems, but in STEM we see a distortion. } I uploaded a picture* of the problem. } We suspect some magnetic field could be responsible for this } behavior. } } What do you think? And what could we do to find the reason? } } Thank you in advance! } } best regards, } } Max } } } * https://www.dropbox.com/s/1ued2rl94y81rfo/19-02-2014-80kV_HAADF.jpg } } ==============================Original } Headers============================== } 8, 21 -- From raven2020-at-gmx.de Wed Feb 19 08:36:53 2014 } 8, 21 -- Received: from mout.gmx.net (mout.gmx.net [212.227.15.18]) } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s1JEaqrx003083 } 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 08:36:53 } -0600 } 8, 21 -- Received: from [172.16.44.168] ([192.124.253.68]) by } mail.gmx.com (mrgmx001) } 8, 21 -- with ESMTPSA (Nemesis) id 0MQiVh-1Whspq3RwD-00U3TL for } 8, 21 -- {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 15:36:51 +0100 } 8, 21 -- Message-ID: {5304C181.1080301-at-gmx.de} } 8, 21 -- Date: Wed, 19 Feb 2014 15:36:49 +0100 } 8, 21 -- From: Max Klingsporn {raven2020-at-gmx.de} } 8, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:24.0) } Gecko/20100101 Thunderbird/24.3.0 } 8, 21 -- MIME-Version: 1.0 } 8, 21 -- To: microscopy-at-microscopy.com } 8, 21 -- Subject: Problem with STEM at 80kV } 8, 21 -- X-Enigmail-Version: 1.6 } 8, 21 -- Content-Type: text/plain; charset=UTF-8 } 8, 21 -- Content-Transfer-Encoding: 8bit } 8, 21 -- X-Provags-ID: } V03:K0:Jd85cpcfmJhK89zoT/+VwZUNCcLvN10eO4UDzOBl0xw5Xk6fWbR } 8, 21 -- } PMGPjbOUv7YqJ6XEmvSm8ErxIMqdNRahX8NUt7t8IjN2f77twuLS6zeYUp2QR+1z0pgKwUi } 8, 21 -- } HzXZk+N70BRYGOw7z1hdGfvnQw3YipD6OVw01YMu/SUeRv4q6PkTfrFfwRYOFyir5SP2y/y } 8, 21 -- SdCfgF68P5Em3S6Pj7KfA== } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 31 -- From trh-at-uoregon.edu Wed Feb 19 19:37:51 2014 4, 31 -- Received: from smtp.uoregon.edu (oh-mserv2.uoregon.edu [128.223.142.145]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1K1bpE9019685 4, 31 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 19:37:51 -0600 4, 31 -- Received: from webmail.uoregon.edu (webmail1.uoregon.edu [128.223.143.235] (may be forged)) 4, 31 -- (authenticated bits=0) 4, 31 -- by smtp.uoregon.edu (8.14.7/8.14.5) with ESMTP id s1K1bjqk019259 4, 31 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 4, 31 -- Wed, 19 Feb 2014 17:37:45 -0800 4, 31 -- Received: from [2001:468:d01:14:223:aeff:fe71:fe9d] 4, 31 -- by webmail.uoregon.edu 4, 31 -- with HTTP (HTTP/1.1 POST); Wed, 19 Feb 2014 17:37:44 -0800 4, 31 -- MIME-Version: 1.0 4, 31 -- Content-Type: text/plain; charset=UTF-8; 4, 31 -- format=flowed 4, 31 -- Content-Transfer-Encoding: 8bit 4, 31 -- Date: Wed, 19 Feb 2014 17:37:44 -0800 4, 31 -- From: Tyler R Harvey {trh-at-uoregon.edu} 4, 31 -- To: raven2020-at-gmx.de 4, 31 -- Cc: microscopy-at-microscopy.com 4, 31 -- Subject: Re: [Microscopy] Problem with STEM at 80kV 4, 31 -- In-Reply-To: {201402191450.s1JEoOAI021491-at-ns.microscopy.com} 4, 31 -- References: {201402191450.s1JEoOAI021491-at-ns.microscopy.com} 4, 31 -- Message-ID: {94c5a17e18192630ad49aeb3e0d52338-at-uoregon.edu} 4, 31 -- X-Sender: trh-at-uoregon.edu 4, 31 -- User-Agent: Roundcube Webmail/0.9.5 4, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.11.87,1.0.14,0.0.0000 4, 31 -- definitions=2014-02-19_07:2014-02-18,2014-02-19,1970-01-01 signatures=0 4, 31 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=4 phishscore=0 4, 31 -- adultscore=0 bulkscore=0 classifier=spam adjust=-40 reason=mlx scancount=1 4, 31 -- engine=7.0.1-1305240000 definitions=main-1402190177 ==============================End of - Headers==============================
Good evening everyone, I am have some problem with my formvar grids. till 6 months ago they were all good but now all (i even checked new one from sealed box) of them are extremely fragile under beam (they tear apart even while going from low mag to high mag mode). Not only that, it seems they are developing some sort of static charge as beam shifts can be seen inside microscope when i insert those grids. here is attached pics of how a beam edge (a shadow of objective aperture) is behaving near a hole (made during changing mode) in formvar substrate. Any ideas what am doing wrong?
https://sites.google.com/site/auxilliarylinks/
Regards Amit Gupta Research Scholar Dept. of Inorganic and Physical Chemistry, IISc
==============================Original Headers============================== 3, 26 -- From amit.welcomes.u-at-gmail.com Wed Feb 19 23:51:11 2014 3, 26 -- Received: from mail-wg0-f43.google.com (mail-wg0-f43.google.com [74.125.82.43]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1K5pBpx009868 3, 26 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 23:51:11 -0600 3, 26 -- Received: by mail-wg0-f43.google.com with SMTP id a1so1104068wgh.22 3, 26 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2014 21:51:10 -0800 (PST) 3, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=20120113; 3, 26 -- h=mime-version:from:date:message-id:subject:to:content-type; 3, 26 -- bh=V4MuM70me0UbYmQ4M3YN3oNvI4WgIWNjq2t6dr/q/Mk=; 3, 26 -- b=L8whLVOdL5W+peLBxu5ePqqBQdi8vpDFJb3nvqqJsEQPz9XgVGVi1XVnF5rgOwa4KJ 3, 26 -- 5Ux++dOK9YIWgShojXU9akCxi/K9JBiRj/n7gKJY6P+vBzGrm2LcinxnDmjTU4gVPs5v 3, 26 -- Je1+vYx3aIfDBPEpyhzEyNfEoIGnktCDOM8LgrEhkoGz/+xrEVYG0T2byE37yP62XzIv 3, 26 -- vrFQBABWnsRq2UCWmVOg/3eASwe5exoF9kpcbbVD3ko2+QuEqbRIN9tGL1ad8XABqIgv 3, 26 -- ynjjpZirhu6nPPOhQenu60zv0DRLs9aCkA1ghLsCWiOrBgYgzLaPR+qPX4OEPq/B6vVw 3, 26 -- vGsQ== 3, 26 -- X-Received: by 10.194.63.228 with SMTP id j4mr32514004wjs.34.1392875470300; 3, 26 -- Wed, 19 Feb 2014 21:51:10 -0800 (PST) 3, 26 -- MIME-Version: 1.0 3, 26 -- Received: by 10.194.179.202 with HTTP; Wed, 19 Feb 2014 21:50:50 -0800 (PST) 3, 26 -- From: Amit Gupta {amit.welcomes.u-at-gmail.com} 3, 26 -- Date: Thu, 20 Feb 2014 11:20:50 +0530 3, 26 -- Message-ID: {CA+bx8_zpVtgSFU9SYmQMQnzOjJohAs-it0y5foXUi5NJnN1hKw-at-mail.gmail.com} 3, 26 -- Subject: Formvar grids having static? 3, 26 -- To: microscopy {microscopy-at-microscopy.com} 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
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Email: kristian.molhave-at-nanotech.dtu.dk Name: Kristian Molhave
Organization: Technical University of Denmark, Dept. of Micro and Nanotechnology
Title-Subject: [Filtered] Two PhD positions open in electron microscopy of processes in liquids
Message: The Department of Micro- and Nanotechnology invites applications for two 3-year PhD Scholarships on studying nanoscale processes in liquids with electron microscopy:
PhD scholarship in Electron microscopy of electrochemical processes in liquids
for more information see http://www.nanotech.dtu.dk/english/About-NTCH/Jobs/job?id=0673b787-8118-4951-af3b-2e0925f70d70
and
PhD scholarship in Nanoscale Imaging of the Aqueous Processes of Precipitation, Dispersion, and Imbibition
for more information see http://www.nanotech.dtu.dk/english/About-NTCH/Jobs/job?id=e41ab81b-5adf-4a86-bc5e-ef10cc5610a9
The Deadline for applications is 27 and 28th february.
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RJ Perz-Edwards- I also suspected the same problem hence cleaned the holder properly, though did not check the top part. Well most of grids are little vulnerable during that transition but now they are almost sure to break apart. also if u do stem you can see them expanding and breaking. It reders them almost useless. shashi singh- no we are buying it. Glow discharge is not available at the moment to try but i did try grounding them while making sample... did not help. Will try ethanol shortly. Ross- Although manufacturer is same but they are copper only, we can see it in EDS. Besides they were working perfectly before, how come all of sudden?
Regards Amit Gupta Research Scholar Prof. S.Vasudevan's lab Dept. of Inorganic and Physical Chemistry, IISc
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Dear Listservers, I am planning an HPF hybrid (pre-light chemical fixation) technique on attached cells, for HM-20 post embed IEM. My plan is to lightly fix the cells for 1 hr (4% PF 0.5% Ga 0.1M HEPES with 5mM MgCl2), rinse in 0.1M HEPES 3.5% sucrose with 5mM MgCl2 (243 mOsmols), then scrape and pellet the cells. I will resuspend the pellet in 2% agarose (in the cells media minus serum) just before HPF. My question is has anyone used HEPES as an IEM buffer, and at what concentration (molarity)? I have read that a slightly hypotonic solution is better for IEM, I believe the cultured cells are isotonic at 300 mOsmols. Also for IEM, is there really a difference between 4% PF and 2% PF? Any suggestions are welcome.
sincerely, Michael Delannoy
==============================Original Headers============================== 2, 32 -- From prvs=1211802d7=delannoy-at-jhmi.edu Thu Feb 20 12:40:37 2014 2, 32 -- Received: from IPEX5.johnshopkins.edu (ipex5.johnshopkins.edu [128.220.161.72]) 2, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1KIeb4e013212; 2, 32 -- Thu, 20 Feb 2014 12:40:37 -0600 2, 32 -- X-IronPort-AV: E=Sophos;i="4.97,513,1389762000"; 2, 32 -- d="scan'208";a="38008255" 2, 32 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) 2, 32 -- by IPEX5.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 20 Feb 2014 13:40:37 -0500 2, 32 -- MIME-version: 1.0 2, 32 -- Content-transfer-encoding: 7BIT 2, 32 -- Content-disposition: inline 2, 32 -- Content-type: text/plain; CHARSET=US-ASCII 2, 32 -- Received: from johnshopkins.edu ([unknown] [10.173.7.15]) 2, 32 -- by commsmail.johnshopkins.edu 2, 32 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 2, 32 -- with ESMTP id {0N1B00J6457O8AB0-at-commsmail.johnshopkins.edu} ; Thu, 2, 32 -- 20 Feb 2014 13:40:36 -0500 (EST) 2, 32 -- Received: from [10.173.109.13] (Forwarded-For: [10.16.66.55]) 2, 32 -- by commsmail.johnshopkins.edu (mshttpd); Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 2, 32 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} , 2, 32 -- MSA listserver {Microscopy-at-msa.microscopy.com} , 2, 32 -- "NewSubList-at-Microscopy.com" {NewSubList-at-microscopy.com} 2, 32 -- Message-id: {72408ae2142e.530605d4-at-johnshopkins.edu} 2, 32 -- Date: Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 2, 32 -- 2009) 2, 32 -- Content-language: en 2, 32 -- Subject: HEPES and HPF 2, 32 -- X-Accept-Language: en 2, 32 -- Priority: normal ==============================End of - Headers==============================
Michael - I use HEPES routinely in my regular fix and for immunocytochemistry. I typically use 70 mM NaCl, 30 mM HEPES, 2 mM CaCl2, pH 7.4. I based my choice of osmolarity on the old half-strength Karnovsky's buffer which if I remember was 100 mM cacodylate. You see a lot of people referring to half-strength Karnovsky's as being ~1000 mOsm (or full-strength as being 2010 mOsm). These numbers assume the formaldehyde and glutaraldehyde contribute to the osmolarity of the fix. They might count in an osmometer measurement but if something crosses the membrane it doesn't count to the cell. My buffer is clearly "hypo-osmotic" to a mammalian cell at ~300 mOsm but empirically it seems to cause the least shrinkage or swelling. Your results may vary. Somebody once told me that Karnovsky picked a high aldehyde concentration to show they didn't contribute to the osmolarity but I don't know if that is true.
Is there a difference between 2% PF and 4% PF? Yes, I believe 4% has twice as much PF! Sorry, I couldn't resist the joke. I know exactly what you mean. I haven't seen much difference for cell lines and almost always use 2%. I use 2% PF by itself based on my use of "half-strength" Karnovsky's. The old "full-strength Karnovsky's fix was 4% PF and 5% glutaraldehyde - half-strength cut the aldehyde concentrations in half but not the buffer concentration. I suspect with an excess of aldehyde, the same amount of crosslinking occurs if you wait long enough.
Good luck. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu] Sent: Thursday, February 20, 2014 12:42 PM To: Phillips, Thomas E.
Dear Listservers, I am planning an HPF hybrid (pre-light chemical fixation) technique on attached cells, for HM-20 post embed IEM. My plan is to lightly fix the cells for 1 hr (4% PF 0.5% Ga 0.1M HEPES with 5mM MgCl2), rinse in 0.1M HEPES 3.5% sucrose with 5mM MgCl2 (243 mOsmols), then scrape and pellet the cells. I will resuspend the pellet in 2% agarose (in the cells media minus serum) just before HPF. My question is has anyone used HEPES as an IEM buffer, and at what concentration (molarity)? I have read that a slightly hypotonic solution is better for IEM, I believe the cultured cells are isotonic at 300 mOsmols. Also for IEM, is there really a difference between 4% PF and 2% PF? Any suggestions are welcome.
sincerely, Michael Delannoy
==============================Original Headers============================== 2, 32 -- From prvs=1211802d7=delannoy-at-jhmi.edu Thu Feb 20 12:40:37 2014 2, 32 -- Received: from IPEX5.johnshopkins.edu (ipex5.johnshopkins.edu [128.220.161.72]) 2, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1KIeb4e013212; 2, 32 -- Thu, 20 Feb 2014 12:40:37 -0600 2, 32 -- X-IronPort-AV: E=Sophos;i="4.97,513,1389762000"; 2, 32 -- d="scan'208";a="38008255" 2, 32 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) 2, 32 -- by IPEX5.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 20 Feb 2014 13:40:37 -0500 2, 32 -- MIME-version: 1.0 2, 32 -- Content-transfer-encoding: 7BIT 2, 32 -- Content-disposition: inline 2, 32 -- Content-type: text/plain; CHARSET=US-ASCII 2, 32 -- Received: from johnshopkins.edu ([unknown] [10.173.7.15]) 2, 32 -- by commsmail.johnshopkins.edu 2, 32 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 2, 32 -- with ESMTP id {0N1B00J6457O8AB0-at-commsmail.johnshopkins.edu} ; Thu, 2, 32 -- 20 Feb 2014 13:40:36 -0500 (EST) 2, 32 -- Received: from [10.173.109.13] (Forwarded-For: [10.16.66.55]) 2, 32 -- by commsmail.johnshopkins.edu (mshttpd); Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 2, 32 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} , 2, 32 -- MSA listserver {Microscopy-at-msa.microscopy.com} , 2, 32 -- "NewSubList-at-Microscopy.com" {NewSubList-at-microscopy.com} 2, 32 -- Message-id: {72408ae2142e.530605d4-at-johnshopkins.edu} 2, 32 -- Date: Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 2, 32 -- 2009) 2, 32 -- Content-language: en 2, 32 -- Subject: HEPES and HPF 2, 32 -- X-Accept-Language: en 2, 32 -- Priority: normal ==============================End of - Headers==============================
==============================Original Headers============================== 14, 32 -- From PhillipsT-at-missouri.edu Thu Feb 20 17:19:15 2014 14, 32 -- Received: from um-tip2-missouri-out.um.umsystem.edu (um-tip2-missouri-out.um.umsystem.edu [198.209.49.149]) 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1KNJEqM009106 14, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 17:19:15 -0600 14, 32 -- X-IronPort-Anti-Spam-Filtered: true 14, 32 -- X-IronPort-Anti-Spam-Result: AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQELAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP4Iq 14, 32 -- X-IPAS-Result: AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQELAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP4Iq 14, 32 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) 14, 32 -- by um-tip2-exch-relay.um.umsystem.edu with ESMTP; 20 Feb 2014 17:19:13 -0600 14, 32 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) by 14, 32 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi id 14.03.0174.001; 14, 32 -- Thu, 20 Feb 2014 17:19:13 -0600 14, 32 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 14, 32 -- To: "delannoy-at-jhmi.edu" {delannoy-at-jhmi.edu} , 14, 32 -- "'microscopy-at-microscopy.com'" 14, 32 -- {microscopy-at-microscopy.com} 14, 32 -- Subject: RE: [Microscopy] HEPES and HPF 14, 32 -- Thread-Topic: [Microscopy] HEPES and HPF 14, 32 -- Thread-Index: AQHPLmtlpsZ4x+1j6Eefm0lyGFg1L5q+wryg 14, 32 -- Date: Thu, 20 Feb 2014 23:19:13 +0000 14, 32 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8ED37E5-at-UM-MBX-T02.um.umsystem.edu} 14, 32 -- References: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} 14, 32 -- In-Reply-To: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} 14, 32 -- Accept-Language: en-US 14, 32 -- Content-Language: en-US 14, 32 -- X-MS-Has-Attach: 14, 32 -- X-MS-TNEF-Correlator: 14, 32 -- x-originating-ip: [128.206.81.115] 14, 32 -- Content-Type: text/plain; charset="us-ascii" 14, 32 -- MIME-Version: 1.0 14, 32 -- Content-Transfer-Encoding: 8bit 14, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1KNJEqM009106 ==============================End of - Headers==============================
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==============================Original Headers============================== 12, 29 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 20 18:13:46 2014 12, 29 -- Received: from znl.com ([206.69.208.20]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1L0DkLr028564 12, 29 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 18:13:46 -0600 12, 29 -- Received: from localhost (localhost [127.0.0.1]) 12, 29 -- by znl.com (Postfix) with ESMTP id 1CA596C200F 12, 29 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 18:13:46 -0600 (CST) 12, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 29 -- Received: from znl.com ([127.0.0.1]) 12, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 29 -- with ESMTP id 70urhNz2Hm4S for {microscopy-at-microscopy.com} ; 12, 29 -- Thu, 20 Feb 2014 18:13:42 -0600 (CST) 12, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 29 -- by znl.com (Postfix) with ESMTPA id D92886C2002 12, 29 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 18:13:42 -0600 (CST) 12, 29 -- Message-ID: {53069A36.8050208-at-microscopy.com} 12, 29 -- Date: Thu, 20 Feb 2014 18:13:42 -0600 12, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 29 -- MIME-Version: 1.0 12, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 29 -- Subject: viaWWW:An Analytical Scientist position open in BP Petrochemical 12, 29 -- Technology unit 12, 29 -- References: {201402201738.s1KHc26n012004-at-ns.microscopy.com} 12, 29 -- In-Reply-To: {201402201738.s1KHc26n012004-at-ns.microscopy.com} 12, 29 -- X-Forwarded-Message-Id: {201402201738.s1KHc26n012004-at-ns.microscopy.com} 12, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I thank Prof. Phillips for the thorough explanation of his opinion on the matter. I also would like to second use of 2%PFA (FA made from paraformaldehyde powder right before)instead of 4% for cell cultures/cultured cells. I have read papers stating 30 min. initial fixation ( -at-room temperature ) would be sufficient for cultured cells. Needless to say it would be of advantage to block unreacted aldehydes after fixation by washes in your buffer + 50mM NH4Cl (ROTH J et al, Enhancement of Structural Preservation and Immunohistochemical Staining in low temperature embedded pancreatic tissue, JHC 29,663-671(1981)
I have seen also an interesting post (on ResearchGate, www.researchgate.net, Thread: http://www.researchgate.net/topic/Histology/post/Alternatives_to_formalin_fixation Re by Thomas Kroneis . Medizinische Universität Graz = Med. Univ. Graz, Austria) on using HOPE(R) as an alternative for FA-fixation in IHC. HOPE. HOPE = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect T. Kroneis wrote in his post :
{ { ....Additionally to what has been posted above: there is another fixation around: HOPE. HOPE = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect (German: http://dcs-diagnostics.de; http://dcs-diagnostics.de/index.php?sprache=de ; ENGL: http://dcs-diagnostics.de/index.php?sprache=en ),
HOPE(R)-Fixation: GERM: http://dcs-diagnostics.de/index.php?sprache=de&&qid=48 , ENGL: http://dcs-diagnostics.de/index.php?sprache=en&&qid=63 ). This fixation yields as good morphological quality as FFPE material and circumvents the problem with the antibodies not recognizing the epitopes! Now the bad news: You need to place your sample directly into the pre-cooled HOPE I solution, second, it takes you quite some time (fixation 16h - 72h plus another day). - So if there is just no possibility to have it snap frozen, maybe it would be possible to have the respective solution stored there (crucial: at +2°C!). If you do not perform RNA analysis afterwards (do you?), you also might think of having the sample just stored in a tube and transferred to your site (in case of HOPE - just cooling, do not use buffers etc.). Maybe you should also think of getting solved the logical issue. Good science needs perfect conditions (you know: shit in - shit out!) Hope this helped, contact me, if you need further assistance (our lab protocol, ...)} } , and, same thread: Re by: Sebastian Marwitz . Research Center Borstel { { The probably best described alternative to PFA is the HOPE-technique: (Olert et al.,Pathol Res Pract. 2001;197(12):823-6); Wiedorn et al., Pathol Res Pract. 2002;198(11):735-40; Kähler et al., J Histochem Cytochem. 2010 Mar;58(3):221-8; Marwitz et al., J Histochem Cytochem. 2011 Jun;59(6):601-14.; Vollmer et al., Rom J Morphol Embryol. 2006;47(1):15-9; Goldmann et al., Pathol Res Pract. 2005;201(8-9):599-602.; Goldmann et al., Am J Pathol. 2003 Dec;163(6):2638-40) . It is a non-crosslinking fixation with acetone dehydration und subsequent paraffin-embedding. There is compared to PFA only minimal denaturation of nucleic acids and allows a multitude of read-outs from one tissue/cell block (qPCR, proteomics, RNA in situ hybridization, microarrays, ...). Most important, you don´t need any antigen-retrieval and are able to dilute your primary antibodies very high. If you are skilled in handling PFA-fixed specimen, there will be no big difference in tissue processing and cutting. End of citation} }
Also I have a pdf in my files, written by T.W. BRIMBLE: The developing Role of the Zwitterionic Buffer (1981) wherein use of HEPES buffer in various combinations or molar concentrations in Cell/organ culture, bacterial culture, blood proteins, cryogenic tissue storage and chemical data will be presented and discussed. Since at the beginning of the "era of zwitterionic buffers" there is only a small subtitle "EM" showing interesting effects in fixation of plant material (total 48 references, file= 5,5MB). So if anyone is interested, I could send that file to any requester..
Have a nice and warm weekend, especially in the US,
best regards, Wolfgang
Wolfgang MUSS EM-Lab Univ.Inst.Pathology SALK-LKH(Gen. Hosp.) and PMU (Private Paracelsus Medical University SALZBURG) SALZBURG-AUSTRIA DISCLAIMER: no affiliation, or financial interest in any of the companies or their products.
} -----Ursprüngliche Nachricht----- } Von: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] } Gesendet: Freitag, 21. Februar 2014 00:25 } An: Muß Wolfgang } Betreff: [Microscopy] RE: HEPES and HPF } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } (Re: PhillipsT-at-missouri.edu) } Michael - I use HEPES routinely in my regular fix and for } immunocytochemistry. I typically use 70 mM NaCl, 30 mM HEPES, 2 mM } CaCl2, pH 7.4. I based my choice of osmolarity on the old half-strength } Karnovsky's buffer which if I remember was 100 mM cacodylate. You see a } lot of people referring to half-strength Karnovsky's as being ~1000 } mOsm (or full-strength as being 2010 mOsm). These numbers assume the } formaldehyde and glutaraldehyde contribute to the osmolarity of the } fix. They might count in an osmometer measurement but if something } crosses the membrane it doesn't count to the cell. My buffer is clearly } "hypo-osmotic" to a mammalian cell at ~300 mOsm but empirically it } seems to cause the least shrinkage or swelling. Your results may vary. } Somebody once told me that Karnovsky picked a high aldehyde } concentration to show they didn't contribute to the osmolarity but I } don't know if that is true. } } Is there a difference between 2% PF and 4% PF? Yes, I believe 4% has } twice as much PF! Sorry, I couldn't resist the joke. I know exactly } what you mean. I haven't seen much difference for cell lines and almost } always use 2%. I use 2% PF by itself based on my use of "half-strength" } Karnovsky's. The old "full-strength Karnovsky's fix was 4% PF and 5% } glutaraldehyde - half-strength cut the aldehyde concentrations in half } but not the buffer concentration. I suspect with an excess of aldehyde, } the same amount of crosslinking occurs if you wait long enough. } } Good luck. Tom } } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://biology.missouri.edu/people/?person=88 } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu] } Sent: Thursday, February 20, 2014 12:42 PM } To: Phillips, Thomas E. } Subject: [Microscopy] HEPES and HPF } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Dear Listservers, } I am planning an HPF hybrid (pre-light chemical fixation) technique on } attached cells, for HM-20 post embed IEM. My plan is to lightly fix } the cells for 1 hr (4% PF 0.5% Ga 0.1M HEPES with 5mM MgCl2), rinse in } 0.1M HEPES 3.5% sucrose with 5mM MgCl2 (243 mOsmols), then scrape and } pellet the cells. I will resuspend the pellet in 2% agarose (in the } cells media minus serum) just before HPF. My question is has anyone } used HEPES as an IEM buffer, and at what concentration (molarity)? I } have read that a slightly hypotonic solution is better for IEM, I } believe the cultured cells are isotonic at 300 mOsmols. Also for IEM, } is there really a difference between 4% PF and 2% PF? Any suggestions } are welcome. } } sincerely, } Michael Delannoy } } ==============================Original } Headers============================== } 2, 32 -- From prvs=1211802d7=delannoy-at-jhmi.edu Thu Feb 20 12:40:37 2014 } 2, 32 -- Received: from IPEX5.johnshopkins.edu (ipex5.johnshopkins.edu } [128.220.161.72]) } 2, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s1KIeb4e013212; } 2, 32 -- Thu, 20 Feb 2014 12:40:37 -0600 } 2, 32 -- X-IronPort-AV: E=Sophos;i="4.97,513,1389762000"; } 2, 32 -- d="scan'208";a="38008255" } 2, 32 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) } 2, 32 -- by IPEX5.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 20 Feb } 2014 13:40:37 -0500 } 2, 32 -- MIME-version: 1.0 } 2, 32 -- Content-transfer-encoding: 7BIT 2, 32 -- Content-disposition: } inline 2, 32 -- Content-type: text/plain; CHARSET=US-ASCII 2, 32 -- } Received: from johnshopkins.edu ([unknown] [10.173.7.15]) 2, 32 -- by } commsmail.johnshopkins.edu 2, 32 -- (Sun Java(tm) System Messaging } Server 7u3-12.01 64bit (built Oct 15 2009)) 2, 32 -- with ESMTP id } {0N1B00J6457O8AB0-at-commsmail.johnshopkins.edu} ; Thu, 2, 32 -- 20 Feb } 2014 13:40:36 -0500 (EST) 2, 32 -- Received: from [10.173.109.13] } (Forwarded-For: [10.16.66.55]) 2, 32 -- by commsmail.johnshopkins.edu } (mshttpd); Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- From: Michael J } Delannoy {delannoy-at-jhmi.edu} 2, 32 -- To: "microscopy-at-microscopy.com" } {microscopy-at-microscopy.com} , } 2, 32 -- "microscopy-at-msa.microscopy.com" } {microscopy-at-msa.microscopy.com} , } 2, 32 -- MSA listserver {Microscopy-at-msa.microscopy.com} , } 2, 32 -- "NewSubList-at-Microscopy.com" } {NewSubList-at-microscopy.com} } 2, 32 -- Message-id: {72408ae2142e.530605d4-at-johnshopkins.edu} } 2, 32 -- Date: Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- X-Mailer: Sun } Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 2, 32 - } - 2009) 2, 32 -- Content-language: en 2, 32 -- Subject: HEPES and HPF } 2, 32 -- X-Accept-Language: en 2, 32 -- Priority: normal } ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 14, 32 -- From PhillipsT-at-missouri.edu Thu Feb 20 17:19:15 2014 } 14, 32 -- Received: from um-tip2-missouri-out.um.umsystem.edu (um-tip2- } missouri-out.um.umsystem.edu [198.209.49.149]) } 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s1KNJEqM009106 } 14, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 17:19:15 } -0600 } 14, 32 -- X-IronPort-Anti-Spam-Filtered: true } 14, 32 -- X-IronPort-Anti-Spam-Result: } AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQE } LAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP4 } Iq } 14, 32 -- X-IPAS-Result: } AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQE } LAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP4 } Iq } 14, 32 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) } 14, 32 -- by um-tip2-exch-relay.um.umsystem.edu with ESMTP; 20 Feb } 2014 17:19:13 -0600 } 14, 32 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) } by } 14, 32 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi id } 14.03.0174.001; } 14, 32 -- Thu, 20 Feb 2014 17:19:13 -0600 } 14, 32 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 14, 32 -- To: "delannoy-at-jhmi.edu" {delannoy-at-jhmi.edu} , } 14, 32 -- "'microscopy-at-microscopy.com'" } 14, 32 -- {microscopy-at-microscopy.com} } 14, 32 -- Subject: RE: [Microscopy] HEPES and HPF } 14, 32 -- Thread-Topic: [Microscopy] HEPES and HPF } 14, 32 -- Thread-Index: AQHPLmtlpsZ4x+1j6Eefm0lyGFg1L5q+wryg } 14, 32 -- Date: Thu, 20 Feb 2014 23:19:13 +0000 } 14, 32 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DD8ED37E5-at-UM-MBX- } T02.um.umsystem.edu} } 14, 32 -- References: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} } 14, 32 -- In-Reply-To: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} } 14, 32 -- Accept-Language: en-US } 14, 32 -- Content-Language: en-US } 14, 32 -- X-MS-Has-Attach: } 14, 32 -- X-MS-TNEF-Correlator: } 14, 32 -- x-originating-ip: [128.206.81.115] } 14, 32 -- Content-Type: text/plain; charset="us-ascii" } 14, 32 -- MIME-Version: 1.0 } 14, 32 -- Content-Transfer-Encoding: 8bit } 14, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s1KNJEqM009106 } ==============================End of - } Headers==============================
==============================Original Headers============================== 14, 38 -- From W.Muss-at-salk.at Fri Feb 21 03:39:09 2014 14, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 14, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1L9d8i8021016 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 03:39:08 -0600 14, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 14, 38 -- by hermes.salk.at (Postfix) with ESMTP id 3A988646B3F 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- X-Scanned-By: SALK-Content-Filter 14, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 14, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 14, 38 -- with ESMTP id QpnRWQ6Q869V for {microscopy-at-microscopy.com} ; 14, 38 -- Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 14, 38 -- by hermes.salk.at (Postfix) with ESMTP id 0AB3B646B39 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 14, 38 -- by N2EX199.lks.local with ESMTP; 21 Feb 2014 10:43:18 +0100 14, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 14, 38 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 14, 38 -- 14.02.0387.000; Fri, 21 Feb 2014 10:38:44 +0100 14, 38 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 14, 38 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 14, 38 -- Subject: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) 14, 38 -- Thread-Topic: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) 14, 38 -- Thread-Index: AQHPLpLyiInVfqdn5UaTlA2X/RENBpq/Z+oQ 14, 38 -- Date: Fri, 21 Feb 2014 09:38:43 +0000 14, 38 -- Message-ID: {3D22E6EE65F39E448DCE4162815376711C5FBCA4-at-N2DT131.lksdom21.lks.local} 14, 38 -- References: {201402202324.s1KNOY1E013491-at-ns.microscopy.com} 14, 38 -- In-Reply-To: {201402202324.s1KNOY1E013491-at-ns.microscopy.com} 14, 38 -- Accept-Language: de-DE, en-US 14, 38 -- Content-Language: de-DE 14, 38 -- X-MS-Has-Attach: 14, 38 -- X-MS-TNEF-Correlator: 14, 38 -- x-originating-ip: [192.168.42.2] 14, 38 -- Content-Type: text/plain; charset="iso-8859-1" 14, 38 -- MIME-Version: 1.0 14, 38 -- Content-Transfer-Encoding: 8bit 14, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1L9d8i8021016 ==============================End of - Headers==============================
A while ago while working on the histology of naturally distended rabbit urinary bladders that had been subjected to outlet obstruction, I used a (P)HCHO at 4% in a buffer (probably phosphate) at 4 degrees C as a 'quick' fix (10 minutes) of the whole, distended, bladder followed by removal of the urine and replacement with more cold buffered (P)HCHO to further fix the mucosal side of the bladder wall. The point here is that the 10 minute immersion 'froze' the smooth muscle so that the bladder did not contract when removing the urine and replacing with fixative.
This method worked as well on muscle that had been used in physiologic contraction studies to preclude contraction when the tissue was stimulated/studied in Hank's Balanced Salt Solution rather than in traditional phosphate or Tyrode buffer. The problem is that Hank's is not approved via general use, but if you want your muscle to look like muscle when you are done, something like Hank's is helpful.
As to the concentration of HCHO, it is my belief that all histologic protocols support/require the use of excesses of formaldehyde, and everything else. This is an edgy topic, and likely not worth too much time. The key issue with all methods of tissue preservation lies with the necessity to avoid having to provide an explanation for why one's tissue looks so bad!!! What IS/WAS liver must LOOK like liver!
Cheers, and hope this starts arguments,
Fred Monson.
What else does one do when one gets old other than get cranky?
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmonson-at-wcupa.edu
HomePage: http://cmirt.wcupa.edu (with link to instrument Scheduler)
-----Original Message----- X-from: W.Muss-at-salk.at [mailto:W.Muss-at-salk.at] Sent: Friday, February 21, 2014 4:49 AM To: Monson, Frederick
Dear Michael, dear all,
I thank Prof. Phillips for the thorough explanation of his opinion on the matter. I also would like to second use of 2%PFA (FA made from paraformaldehyde powder right before)instead of 4% for cell cultures/cultured cells. I have read papers stating 30 min. initial fixation ( -at-room temperature ) would be sufficient for cultured cells. Needless to say it would be of advantage to block unreacted aldehydes after fixation by washes in your buffer + 50mM NH4Cl (ROTH J et al, Enhancement of Structural Preservation and Immunohistochemical Staining in low temperature embedded pancreatic tissue, JHC 29,663-671(1981)
I have seen also an interesting post (on ResearchGate, www.researchgate.net, Thread: http://www.researchgate.net/topic/Histology/post/Alternatives_to_formalin_fixation Re by Thomas Kroneis . Medizinische Universität Graz = Med. Univ. Graz, Austria) on using HOPE(R) as an alternative for FA-fixation in IHC. HOPE. HOPE = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect T. Kroneis wrote in his post :
{ { ....Additionally to what has been posted above: there is another fixation around: HOPE. HOPE = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect (German: http://dcs-diagnostics.de; http://dcs-diagnostics.de/index.php?sprache=de ; ENGL: http://dcs-diagnostics.de/index.php?sprache=en ),
HOPE(R)-Fixation: GERM: http://dcs-diagnostics.de/index.php?sprache=de&&qid=48 , ENGL: http://dcs-diagnostics.de/index.php?sprache=en&&qid=63 ). This fixation yields as good morphological quality as FFPE material and circumvents the problem with the antibodies not recognizing the epitopes! Now the bad news: You need to place your sample directly into the pre-cooled HOPE I solution, second, it takes you quite some time (fixation 16h - 72h plus another day). - So if there is just no possibility to have it snap frozen, maybe it would be possible to have the respective solution stored there (crucial: at +2°C!). If you do not perform RNA analysis afterwards (do you?), you also might think of having the sample just stored in a tube and transferred to your site (in case of HOPE - just cooling, do not use buffers etc.). Maybe you should also think of getting solved the logical issue. Good science needs perfect conditions (you know: shit in - shit out!) Hope this helped, contact me, if you need further assistance (our lab protocol, ...)} } , and, same thread: Re by: Sebastian Marwitz . Research Center Borstel { { The probably best described alternative to PFA is the HOPE-technique: (Olert et al.,Pathol Res Pract. 2001;197(12):823-6); Wiedorn et al., Pathol Res Pract. 2002;198(11):735-40; Kähler et al., J Histochem Cytochem. 2010 Mar;58(3):221-8; Marwitz et al., J Histochem Cytochem. 2011 Jun;59(6):601-14.; Vollmer et al., Rom J Morphol Embryol. 2006;47(1):15-9; Goldmann et al., Pathol Res Pract. 2005;201(8-9):599-602.; Goldmann et al., Am J Pathol. 2003 Dec;163(6):2638-40) . It is a non-crosslinking fixation with acetone dehydration und subsequent paraffin-embedding. There is compared to PFA only minimal denaturation of nucleic acids and allows a multitude of read-outs from one tissue/cell block (qPCR, proteomics, RNA in situ hybridization, microarrays, ...). Most important, you don´t need any antigen-retrieval and are able to dilute your primary antibodies very high. If you are skilled in handling PFA-fixed specimen, there will be no big difference in tissue processing and cutting. End of citation} }
Also I have a pdf in my files, written by T.W. BRIMBLE: The developing Role of the Zwitterionic Buffer (1981) wherein use of HEPES buffer in various combinations or molar concentrations in Cell/organ culture, bacterial culture, blood proteins, cryogenic tissue storage and chemical data will be presented and discussed. Since at the beginning of the "era of zwitterionic buffers" there is only a small subtitle "EM" showing interesting effects in fixation of plant material (total 48 references, file= 5,5MB). So if anyone is interested, I could send that file to any requester..
Have a nice and warm weekend, especially in the US,
best regards, Wolfgang
Wolfgang MUSS EM-Lab Univ.Inst.Pathology SALK-LKH(Gen. Hosp.) and PMU (Private Paracelsus Medical University SALZBURG) SALZBURG-AUSTRIA DISCLAIMER: no affiliation, or financial interest in any of the companies or their products.
} -----Ursprüngliche Nachricht----- } Von: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] } Gesendet: Freitag, 21. Februar 2014 00:25 } An: Muß Wolfgang } Betreff: [Microscopy] RE: HEPES and HPF } } } } } ---------------------------------------------------------------------- } - } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } - } ----- } (Re: PhillipsT-at-missouri.edu) } Michael - I use HEPES routinely in my regular fix and for } immunocytochemistry. I typically use 70 mM NaCl, 30 mM HEPES, 2 mM } CaCl2, pH 7.4. I based my choice of osmolarity on the old } half-strength Karnovsky's buffer which if I remember was 100 mM } cacodylate. You see a lot of people referring to half-strength } Karnovsky's as being ~1000 mOsm (or full-strength as being 2010 } mOsm). These numbers assume the formaldehyde and glutaraldehyde } contribute to the osmolarity of the fix. They might count in an } osmometer measurement but if something crosses the membrane it doesn't } count to the cell. My buffer is clearly "hypo-osmotic" to a mammalian } cell at ~300 mOsm but empirically it seems to cause the least shrinkage or swelling. Your results may vary. } Somebody once told me that Karnovsky picked a high aldehyde } concentration to show they didn't contribute to the osmolarity but I } don't know if that is true. } } Is there a difference between 2% PF and 4% PF? Yes, I believe 4% has } twice as much PF! Sorry, I couldn't resist the joke. I know exactly } what you mean. I haven't seen much difference for cell lines and } almost always use 2%. I use 2% PF by itself based on my use of "half-strength" } Karnovsky's. The old "full-strength Karnovsky's fix was 4% PF and 5% } glutaraldehyde - half-strength cut the aldehyde concentrations in half } but not the buffer concentration. I suspect with an excess of } aldehyde, the same amount of crosslinking occurs if you wait long enough. } } Good luck. Tom } } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://biology.missouri.edu/people/?person=88 } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu] } Sent: Thursday, February 20, 2014 12:42 PM } To: Phillips, Thomas E. } Subject: [Microscopy] HEPES and HPF } } } } } ---------------------------------------------------------------------- } - } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } - } ----- } } Dear Listservers, } I am planning an HPF hybrid (pre-light chemical fixation) technique on } attached cells, for HM-20 post embed IEM. My plan is to lightly fix } the cells for 1 hr (4% PF 0.5% Ga 0.1M HEPES with 5mM MgCl2), rinse in } 0.1M HEPES 3.5% sucrose with 5mM MgCl2 (243 mOsmols), then scrape and } pellet the cells. I will resuspend the pellet in 2% agarose (in the } cells media minus serum) just before HPF. My question is has anyone } used HEPES as an IEM buffer, and at what concentration (molarity)? I } have read that a slightly hypotonic solution is better for IEM, I } believe the cultured cells are isotonic at 300 mOsmols. Also for IEM, } is there really a difference between 4% PF and 2% PF? Any suggestions } are welcome. } } sincerely, } Michael Delannoy } } ==============================Original } Headers============================== } 2, 32 -- From prvs=1211802d7=delannoy-at-jhmi.edu Thu Feb 20 12:40:37 } 2014 2, 32 -- Received: from IPEX5.johnshopkins.edu } (ipex5.johnshopkins.edu } [128.220.161.72]) } 2, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s1KIeb4e013212; } 2, 32 -- Thu, 20 Feb 2014 12:40:37 -0600 } 2, 32 -- X-IronPort-AV: E=Sophos;i="4.97,513,1389762000"; } 2, 32 -- d="scan'208";a="38008255" } 2, 32 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) } 2, 32 -- by IPEX5.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 20 Feb } 2014 13:40:37 -0500 } 2, 32 -- MIME-version: 1.0 } 2, 32 -- Content-transfer-encoding: 7BIT 2, 32 -- Content-disposition: } inline 2, 32 -- Content-type: text/plain; CHARSET=US-ASCII 2, 32 -- } Received: from johnshopkins.edu ([unknown] [10.173.7.15]) 2, 32 -- by } commsmail.johnshopkins.edu 2, 32 -- (Sun Java(tm) System Messaging } Server 7u3-12.01 64bit (built Oct 15 2009)) 2, 32 -- with ESMTP id } {0N1B00J6457O8AB0-at-commsmail.johnshopkins.edu} ; Thu, 2, 32 -- 20 Feb } 2014 13:40:36 -0500 (EST) 2, 32 -- Received: from [10.173.109.13] } (Forwarded-For: [10.16.66.55]) 2, 32 -- by commsmail.johnshopkins.edu } (mshttpd); Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- From: Michael J } Delannoy {delannoy-at-jhmi.edu} 2, 32 -- To: "microscopy-at-microscopy.com" } {microscopy-at-microscopy.com} , } 2, 32 -- "microscopy-at-msa.microscopy.com" } {microscopy-at-msa.microscopy.com} , } 2, 32 -- MSA listserver {Microscopy-at-msa.microscopy.com} , } 2, 32 -- "NewSubList-at-Microscopy.com" } {NewSubList-at-microscopy.com} } 2, 32 -- Message-id: {72408ae2142e.530605d4-at-johnshopkins.edu} } 2, 32 -- Date: Thu, 20 Feb 2014 13:40:36 -0500 2, 32 -- X-Mailer: Sun } Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 2, 32 } - } - 2009) 2, 32 -- Content-language: en 2, 32 -- Subject: HEPES and HPF } 2, 32 -- X-Accept-Language: en 2, 32 -- Priority: normal } ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 14, 32 -- From PhillipsT-at-missouri.edu Thu Feb 20 17:19:15 2014 14, 32 } -- Received: from um-tip2-missouri-out.um.umsystem.edu (um-tip2- } missouri-out.um.umsystem.edu [198.209.49.149]) } 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s1KNJEqM009106 } 14, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Feb 2014 17:19:15 } -0600 } 14, 32 -- X-IronPort-Anti-Spam-Filtered: true 14, 32 -- } X-IronPort-Anti-Spam-Result: } AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQ } E } LAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP } 4 } Iq } 14, 32 -- X-IPAS-Result: } AgUFAKaMBlPPoJ7Q/2dsb2JhbABWAw6CeDhXwBGBERZ0giUBAQEEOhQlEgICAgEIEQMBAQ } E } LAgIQCQcWGwEUCQgCBAEJCQgNBgIEh2QNxjqGfxcEjGSBSwYbEgUGC4MTgRQEmWKQcoJuP } 4 } Iq } 14, 32 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) } 14, 32 -- by um-tip2-exch-relay.um.umsystem.edu with ESMTP; 20 Feb } 2014 17:19:13 -0600 } 14, 32 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.101]) } by 14, 32 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi } id 14.03.0174.001; 14, 32 -- Thu, 20 Feb 2014 17:19:13 -0600 14, 32 } -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 14, 32 -- To: } "delannoy-at-jhmi.edu" {delannoy-at-jhmi.edu} , } 14, 32 -- "'microscopy-at-microscopy.com'" } 14, 32 -- {microscopy-at-microscopy.com} } 14, 32 -- Subject: RE: [Microscopy] HEPES and HPF 14, 32 -- } Thread-Topic: [Microscopy] HEPES and HPF 14, 32 -- Thread-Index: } AQHPLmtlpsZ4x+1j6Eefm0lyGFg1L5q+wryg } 14, 32 -- Date: Thu, 20 Feb 2014 23:19:13 +0000 14, 32 -- Message-ID: } {CB463A8DE3499A4CA204087E4EEB363DD8ED37E5-at-UM-MBX- } T02.um.umsystem.edu} } 14, 32 -- References: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} } 14, 32 -- In-Reply-To: {201402201841.s1KIfcWP014298-at-ns.microscopy.com} } 14, 32 -- Accept-Language: en-US } 14, 32 -- Content-Language: en-US } 14, 32 -- X-MS-Has-Attach: } 14, 32 -- X-MS-TNEF-Correlator: } 14, 32 -- x-originating-ip: [128.206.81.115] 14, 32 -- Content-Type: } text/plain; charset="us-ascii" } 14, 32 -- MIME-Version: 1.0 } 14, 32 -- Content-Transfer-Encoding: 8bit 14, 32 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s1KNJEqM009106 ==============================End } of - Headers==============================
==============================Original Headers============================== 14, 38 -- From W.Muss-at-salk.at Fri Feb 21 03:39:09 2014 14, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 14, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1L9d8i8021016 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 03:39:08 -0600 14, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 14, 38 -- by hermes.salk.at (Postfix) with ESMTP id 3A988646B3F 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- X-Scanned-By: SALK-Content-Filter 14, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 14, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 14, 38 -- with ESMTP id QpnRWQ6Q869V for {microscopy-at-microscopy.com} ; 14, 38 -- Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 14, 38 -- by hermes.salk.at (Postfix) with ESMTP id 0AB3B646B39 14, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 10:39:07 +0100 (CET) 14, 38 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 14, 38 -- by N2EX199.lks.local with ESMTP; 21 Feb 2014 10:43:18 +0100 14, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 14, 38 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 14, 38 -- 14.02.0387.000; Fri, 21 Feb 2014 10:38:44 +0100 14, 38 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 14, 38 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 14, 38 -- Subject: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) 14, 38 -- Thread-Topic: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) 14, 38 -- Thread-Index: AQHPLpLyiInVfqdn5UaTlA2X/RENBpq/Z+oQ 14, 38 -- Date: Fri, 21 Feb 2014 09:38:43 +0000 14, 38 -- Message-ID: {3D22E6EE65F39E448DCE4162815376711C5FBCA4-at-N2DT131.lksdom21.lks.local} 14, 38 -- References: {201402202324.s1KNOY1E013491-at-ns.microscopy.com} 14, 38 -- In-Reply-To: {201402202324.s1KNOY1E013491-at-ns.microscopy.com} 14, 38 -- Accept-Language: de-DE, en-US 14, 38 -- Content-Language: de-DE 14, 38 -- X-MS-Has-Attach: 14, 38 -- X-MS-TNEF-Correlator: 14, 38 -- x-originating-ip: [192.168.42.2] 14, 38 -- Content-Type: text/plain; charset="iso-8859-1" 14, 38 -- MIME-Version: 1.0 14, 38 -- Content-Transfer-Encoding: 8bit 14, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1L9d8i8021016 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 38 -- From FMonson-at-wcupa.edu Fri Feb 21 12:14:36 2014 28, 38 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.127.4]) 28, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1LIEZcB015735 28, 38 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 12:14:35 -0600 28, 38 -- X-ASG-Debug-ID: 1393006473-0623962cca4f89e0001-4CH8be 28, 38 -- Received: from WCU-XCH-11.PASSHE.LCL (wcu-xch-01.wcupa.edu [144.26.0.85]) by MX01.WCUPA.EDU with ESMTP id khAnABQTHqBKZ5Dm (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2014 13:14:33 -0500 (EST) 28, 38 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 28, 38 -- X-Barracuda-Apparent-Source-IP: 144.26.0.85 28, 38 -- X-ASG-Whitelist: Client 28, 38 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 28, 38 -- WCU-XCH-11.PASSHE.LCL ([fe80::9034:edf3:907e:6463%13]) with mapi id 28, 38 -- 14.03.0123.003; Fri, 21 Feb 2014 13:14:33 -0500 28, 38 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 28, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 28, 38 -- Subject: RE: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) & 28, 38 -- bk to HCHO 28, 38 -- Thread-Topic: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) & 28, 38 -- bk to HCHO 28, 38 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: HEPES and HPF (apologize for lengthiness) & 28, 38 -- bk to HCHO 28, 38 -- Thread-Index: Ac8vLRnbMiOIN/oNSAqok0EwiQUWQA== 28, 38 -- Date: Fri, 21 Feb 2014 18:14:33 +0000 28, 38 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C180BE923-at-WCU-XCH-03.PASSHE.LCL} 28, 38 -- Accept-Language: en-US 28, 38 -- Content-Language: en-US 28, 38 -- X-MS-Has-Attach: 28, 38 -- X-MS-TNEF-Correlator: 28, 38 -- x-originating-ip: [10.28.57.79] 28, 38 -- Content-Type: text/plain; charset="iso-8859-1" 28, 38 -- MIME-Version: 1.0 28, 38 -- X-Barracuda-Connect: wcu-xch-01.wcupa.edu[144.26.0.85] 28, 38 -- X-Barracuda-Start-Time: 1393006473 28, 38 -- X-Barracuda-Encrypted: AES128-SHA 28, 38 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 28, 38 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 28, 38 -- X-Barracuda-BRTS-Status: 1 28, 38 -- Content-Transfer-Encoding: 8bit 28, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1LIEZcB015735 ==============================End of - Headers==============================
From lytcinhu-at-natm.ru Sat Feb 22 04:37:38 2014 Return-Path: {lytcinhu-at-natm.ru} Received: from eth-24-236-81-78-homell.natm.ru ([78.81.236.24]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MAbaeO016449 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 22 Feb 2014 04:37:37 -0600 Message-Id: {201402221037.s1MAbaeO016449-at-ns.microscopy.com}
X-from: wul-at-mail.montclair.edu ()
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wul-at-mail.montclair.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: wul-at-mail.montclair.edu Name: Laying Wu
Organization: education
Title-Subject: [Filtered] Microscopy training workshop 2014
Message: Dear Listservers,
Montclair State University and Hitachi High Technologies Inc. are pleased to announce a low-cost, one-day Light and Electron Microscopy workshop for new and intermediate-level microscopists. The workshop will take place on Friday, March 21, 2014 at Montclair State University, Montclair New Jersey. The workshop will introduce participants to a wide range of the techniques associated with light microscopy, electron microscopy, atomic force microscopy and sample preparation. Guest presenters will join us from Hitachi High Technologies of America, Bruker AXS Inc., and Hitech Instruments Inc. Students, faculty, researchers, and industry users working in biology, geology, environmental science and material science are encouraged to apply.
Please visit the workshop website for further details and registration instructions: http://www.montclair.edu/csam/mmrl/workshop/
Montclair State University is located in Montclair, NJ. We are reachable via the New Jersey Transit train system (Montclair Heights and Montclair State University stations) and by bus. Please visit us at www.montclair.edu.
with best wishes,
Laying Wu
wul-at-mail.montclair.edu TEL: 973-655-2028
Dr. Laying Wu Director, Microscopy and Microanalysis Research Laboratory Montclair State University 1 Normal Ave, Montclair, NJ 07043 USA
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==============================Original Headers============================== 23, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 22 07:47:31 2014 23, 28 -- Received: from znl.com ([206.69.208.20]) 23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MDlVC4019191 23, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:47:31 -0600 23, 28 -- Received: from localhost (localhost [127.0.0.1]) 23, 28 -- by znl.com (Postfix) with ESMTP id C65936C338B 23, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:47:31 -0600 (CST) 23, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 23, 28 -- Received: from znl.com ([127.0.0.1]) 23, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 23, 28 -- with ESMTP id liUjiT8CLSrH for {microscopy-at-microscopy.com} ; 23, 28 -- Sat, 22 Feb 2014 07:47:27 -0600 (CST) 23, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 23, 28 -- by znl.com (Postfix) with ESMTPA id E77C76C3379 23, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:47:27 -0600 (CST) 23, 28 -- Message-ID: {5308AA6F.1060707-at-microscopy.com} 23, 28 -- Date: Sat, 22 Feb 2014 07:47:27 -0600 23, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 23, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 23, 28 -- MIME-Version: 1.0 23, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 23, 28 -- Subject: viaWWW: Microscopy training workshop 2014 at Montclair State University 23, 28 -- References: {201402211954.s1LJsKPO003592-at-ns.microscopy.com} 23, 28 -- In-Reply-To: {201402211954.s1LJsKPO003592-at-ns.microscopy.com} 23, 28 -- X-Forwarded-Message-Id: {201402211954.s1LJsKPO003592-at-ns.microscopy.com} 23, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 23, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both doug.skinner-at-bruker-nano.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Job Opportunity at Bruker - Applications Specialist
Message: Bruker Nano Analytics, a leading manufacturer of Analytical Instrumentation, has a job opening for an Applications Specialist in their Billerica, MA Microanalysis Lab. This division specializes in EDS, WDS, micro-XRF and EBSD on electron microscopes.
The Applications Specialist position is a Sales, Service and Customer support position. The responsibilities are to assist customers, demonstrate the microanalysis instruments and to provide technical sales support for the Microanalysis Product Line.
Primary Responsibilities: * Demonstrate equipment at Bruker and customer sites * Analysis of customer samples * Customer support on-site and via phone/e-mail * User training of the Bruker Microanalysis System * Produce appropriate materials for customer training * Preparation of technical sales materials * Assist Service in resolving customer problems * Perform other tasks as assigned by manager
This position involves up to 50% travel, primarily in North America and Latin America.
Candidates interested in this position require a minimum of a BachelorÂ’s degree in a Physical Science discipline with a degree in Chemistry, Geology or Materials Science preferred. Two years SEM/X-ray microanalysis and EBSD experience, including sample preparation, is preferred.
Requirements include excellent communication skills and proficiency with Microsoft Office. Language: English fluent; preferably Spanish as a second language.
Interested candidates should complete an online application at: www.bruker.com
Bruker Nano Analytics offers a competitive salary and comprehensive benefits package including health and dental insurance, Flexible Spending Account, company sponsored life and disability insurance, 401(k) plan with company matching components, and vacation and sick days.
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 22 07:48:34 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MDmX6M019878 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:48:33 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id B890A6C339A 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:48:33 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id lhsjDKxwAX7u for {microscopy-at-microscopy.com} ; 19, 28 -- Sat, 22 Feb 2014 07:48:31 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 2D90E6C338D 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 07:48:31 -0600 (CST) 19, 28 -- Message-ID: {5308AAAF.90908-at-microscopy.com} 19, 28 -- Date: Sat, 22 Feb 2014 07:48:31 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:Job Opportunity at Bruker - Applications Specialist 19, 28 -- References: {201402212101.s1LL1hT9004383-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201402212101.s1LL1hT9004383-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201402212101.s1LL1hT9004383-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 19, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Dear Colleagues, We would like to bring your attention to the call for abstracts: https://www.avs.org/Meetings-Exhibits/Call-For-Abstracts to the AVS 61st International Symposium & Exhibition (AVS-61), which will be held November 9-14, 2014 in Baltimore, Maryland. It is a good opportunity to explore and report on new developments in microscopy techniques applied to new materials, devices and interfaces. In addition, on the behalf of the organizers, we invite you to submit an abstract before the deadline of Monday, May 5th, to the special topical session "Novel Trends in Synchrotron and FEL-Based Analysis" https://www.avs.org/Meetings-Exhibits/Call-For-Abstracts/Focus-Topics#Synchrotron. This focus session is organized for the first time and aims at dissemination of the unique research opportunities, offered by the advanced experimental techniques at X-ray, VUV or IR synchrotron and free electron laser facilities, to investigate the properties of matter with unprecedented space, spectral and time resolution. One or two more invited presentation will be selected by the program committee evaluating the abstracts. We look forward to receiving your abstract and seeing you November in Baltimore, Maryland! _______________________________________________________________________________ Andrei Kolmakov, PhD Project Leader Center for Nanoscale Science and Technology National Institute of Standards and Technology
Folks - I have two Gatan 600 ion-mills that are up for grabs on Long Island, NY. Both are not 100% working, put contain 90% of parts. Together, you can easily make a working unit. You would have to come here yourself and load (or crate) them yourself.
If nobody offers to take them in their entirety, then anyone is welcome to come here to strip them for parts. I am out that business.
Either way, they are being surplussed in one month.
Next, does anyone have a PDF copy of the instruction manual and schematics for a Gatan 691 ion-mill? thank you and regards, - Jim
==============================Original Headers============================== 4, 26 -- From jquinn11733-at-gmail.com Sat Feb 22 16:10:07 2014 4, 26 -- Received: from mail-la0-f54.google.com (mail-la0-f54.google.com [209.85.215.54]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MMA1mO019338 4, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 16:10:01 -0600 4, 26 -- Received: by mail-la0-f54.google.com with SMTP id mc6so376969lab.27 4, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=20120113; 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 26 -- bh=5t1qAZth4wl4XiRf5nbs2fkeJMdtnubmtEwJi0ZW6bg=; 4, 26 -- b=tpP4pcWiJC0E3IvCxTik4bV6NyQJI8i6RDVWbDE1H3MGWSP+jhxc+fJYbNyvUsFcc/ 4, 26 -- +f0yVsKIO0r53pOsVRONcwG9MMvk+0dlr/ZC0sF8rbCiBoxS0HtyVLQVefZmQlm/VKyN 4, 26 -- MKe3XRKOq0YZ8jpKZCtY9Sxa5b6tt8vkUKkSmcp/2EqZnpoa3E7ghxEQLYkRKFGeryDb 4, 26 -- SJkfdAmU9donJwhgOWFtJdLZb0hBVvnUI467WJmws7SE8mPR0emb84S26RfSH9dJlkwq 4, 26 -- RKVbeHZ7Xy6bbLm4yHWCnj6KB1Ggu4hAtWYV9//yi9J/6oB8XMMW7FYADDbFDMeKltHO 4, 26 -- zXlA== 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Received: by 10.152.205.11 with SMTP id lc11mr7996429lac.29.1393107000474; 4, 26 -- Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- Received: by 10.112.218.3 with HTTP; Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- Date: Sat, 22 Feb 2014 17:10:00 -0500 4, 26 -- Message-ID: {CAO_SM8Kk_7H504-iMRzp+2-eY5tcQZXje=LD6JCp2cneKFNBZQ-at-mail.gmail.com} 4, 26 -- Subject: Gatan 600 ion-mills up for grabs and need manual for 691 4, 26 -- From: Jim Quinn {jquinn11733-at-gmail.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Folks - I have two Gatan 600 ion-mills that are up for grabs on Long Island, NY. Both are not 100% working, put contain 90% of parts. Together, you can easily make a working unit. You would have to come here yourself and load (or crate) them yourself.
If nobody offers to take them in their entirety, then anyone is welcome to come here to strip them for parts. I am out that business.
Either way, they are being surplussed in one month.
Next, does anyone have a PDF copy of the instruction manual and schematics for a Gatan 691 ion-mill? thank you and regards, - Jim
==============================Original Headers============================== 4, 26 -- From jquinn11733-at-gmail.com Sat Feb 22 18:02:24 2014 4, 26 -- Received: from mail-la0-f54.google.com (mail-la0-f54.google.com [209.85.215.54]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1MMA1mO019338 4, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 16:10:01 -0600 4, 26 -- Received: by mail-la0-f54.google.com with SMTP id mc6so376969lab.27 4, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=20120113; 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 26 -- bh=5t1qAZth4wl4XiRf5nbs2fkeJMdtnubmtEwJi0ZW6bg=; 4, 26 -- b=tpP4pcWiJC0E3IvCxTik4bV6NyQJI8i6RDVWbDE1H3MGWSP+jhxc+fJYbNyvUsFcc/ 4, 26 -- +f0yVsKIO0r53pOsVRONcwG9MMvk+0dlr/ZC0sF8rbCiBoxS0HtyVLQVefZmQlm/VKyN 4, 26 -- MKe3XRKOq0YZ8jpKZCtY9Sxa5b6tt8vkUKkSmcp/2EqZnpoa3E7ghxEQLYkRKFGeryDb 4, 26 -- SJkfdAmU9donJwhgOWFtJdLZb0hBVvnUI467WJmws7SE8mPR0emb84S26RfSH9dJlkwq 4, 26 -- RKVbeHZ7Xy6bbLm4yHWCnj6KB1Ggu4hAtWYV9//yi9J/6oB8XMMW7FYADDbFDMeKltHO 4, 26 -- zXlA== 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Received: by 10.152.205.11 with SMTP id lc11mr7996429lac.29.1393107000474; 4, 26 -- Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- Received: by 10.112.218.3 with HTTP; Sat, 22 Feb 2014 14:10:00 -0800 (PST) 4, 26 -- Date: Sat, 22 Feb 2014 17:10:00 -0500 4, 26 -- Message-ID: {CAO_SM8Kk_7H504-iMRzp+2-eY5tcQZXje=LD6JCp2cneKFNBZQ-at-mail.gmail.com} 4, 26 -- Subject: Gatan 600 ion-mills up for grabs and need manual for 691 4, 26 -- From: Jim Quinn {jquinn11733-at-gmail.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jcsmtf-at-mail.missouri.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: I am receiving a Pneumatics message on the screen of our CM10 and I believe this to be the reason while none of the vacuums will start. The air line on our machine runs first to V11, but it has not been allowed past V11. Is there a reason for this? I'm not sure how this valve works, I took it off and it seems to be just a metal unit with no moving parts. I'm guessing the black electrical piece that connects to it has something to do with its operations. Does anyone have any insight on how to open this valve? I am running ~90 psi to it.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Sun Feb 23 08:57:20 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1NEvKo2014694 12, 28 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 08:57:20 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id F2F186C40A5 12, 28 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 08:57:19 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id xJNd9kWLSO3n for {microscopy-at-microscopy.com} ; 12, 28 -- Sun, 23 Feb 2014 08:57:12 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id AECB96C4093 12, 28 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 08:57:12 -0600 (CST) 12, 28 -- Message-ID: {530A0C47.40906-at-microscopy.com} 12, 28 -- Date: Sun, 23 Feb 2014 08:57:11 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:CM10 pneumatics 12, 28 -- References: {201402230100.s1N10rDF021223-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201402230100.s1N10rDF021223-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201402230100.s1N10rDF021223-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Folks - Those two ion-mills are spoken for. Also, I now have the manual for the 691. Thank you Justin and Mike! regards, - Jim
} Folks - } I have two Gatan 600 ion-mills that } are up for grabs on Long Island, NY. } Both are not 100% working, put } contain 90% of parts. Together, } you can easily make a working unit. } You would have to come here yourself } and load (or crate) them yourself. } If nobody offers to take them in their entirety, } then anyone is welcome to come here to } strip them for parts. I am out that business. } Either way, they are being surplussed in one month. } Next, does anyone have a PDF copy of the } instruction manual and schematics for a Gatan 691 } ion-mill? } thank you and regards, } - Jim
==============================Original Headers============================== 3, 26 -- From jquinn11733-at-gmail.com Sun Feb 23 14:28:46 2014 3, 26 -- Received: from mail-la0-f46.google.com (mail-la0-f46.google.com [209.85.215.46]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1NKSkZ6006324 3, 26 -- for {Microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 14:28:46 -0600 3, 26 -- Received: by mail-la0-f46.google.com with SMTP id b8so4575577lan.5 3, 26 -- for {Microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 12:28:45 -0800 (PST) 3, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=20120113; 3, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 26 -- bh=ZS4IwwHqcbgMhJDyqGVJCnleecfyQ7cYVorvgq1PH1c=; 3, 26 -- b=0ADkBfsoL3MIgUCBjH+megm3BV3Xcp+Sl3zwHWU5jpTu3RBh9haYLGwvdd6+1WWFXx 3, 26 -- ckW1JZsgd2Obsb4lxbmCp+LJxYLFUA9+zaxmw8z4T7ZcUM9NWXJBaWB/ESAp7KXWKTxT 3, 26 -- tLo+V1xZmtDvnB8RTk5nJYdOaw7YdGA+q7T7RrZ/Kq/I9Wvu16yf8QoY5AqTgtNFvEYW 3, 26 -- /OYdK+cLEgQuIblm27yNTU/2+mx5YzcAonhav+fyCO9sfSx8o4s112Bzc9/kYo7oL2kG 3, 26 -- DoMC6X74+Nsc8ZTgXoOckeKkITJ12qUzPUzJ73f89ErP0jCZU6EbgquE74FV0mT+Wq9P 3, 26 -- FtUA== 3, 26 -- MIME-Version: 1.0 3, 26 -- X-Received: by 10.153.11.129 with SMTP id ei1mr10054261lad.78.1393187325420; 3, 26 -- Sun, 23 Feb 2014 12:28:45 -0800 (PST) 3, 26 -- Received: by 10.112.218.3 with HTTP; Sun, 23 Feb 2014 12:28:45 -0800 (PST) 3, 26 -- Date: Sun, 23 Feb 2014 15:28:45 -0500 3, 26 -- Message-ID: {CAO_SM8KzJZfTY_hz+maALJ-uQ4a+WGSaVFVnm_242=hHyJPf0g-at-mail.gmail.com} 3, 26 -- Subject: ion-mills are now spoken for 3, 26 -- From: Jim Quinn {jquinn11733-at-gmail.com} 3, 26 -- To: Microscopy-at-microscopy.com 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
The microscope air supply should *NOT* be attached to V11. V11 is the microscope vent valve and is used to release the vacuum in the column and camera chamber. It should never have more than a fraction of a psi supplied to it. Higher pressure can force the viewing chamber window out with great force and cause serious injury to anyone nearby! Venting the column with high pressure air when the sample rod is inserted can also blow the sample rod out with some force and damage it.
The air for the valves is supplied through the "Watts" regulator to the distribution block on the rear of the microscope column. This air ACTIVATES the valves; it doesn't pass through the valve.
Cheers, Henk
On 2/23/2014 9:59 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: jcsmtf-at-mail.missouri.edu () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both jcsmtf-at-mail.missouri.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10 pneumatics } } Message: I am receiving a Pneumatics message on the screen of our CM10 and I believe this to be the } reason while none of the vacuums will start. The air line on our machine runs first to V11, but it } has not been allowed past V11. Is there a reason for this? I'm not sure how this valve works, I took } it off and it seems to be just a metal unit with no moving parts. I'm guessing the black electrical } piece that connects to it has something to do with its operations. Does anyone have any insight on } how to open this valve? I am running ~90 psi to it. } } Login Host: 161.130.188.19 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } ---===[|]===--- } } }
==============================Original Headers============================== 8, 47 -- From colijn.1-at-osu.edu Sun Feb 23 15:19:41 2014 8, 47 -- Received: from va3outboundpool.messaging.microsoft.com (va3ehsobe010.messaging.microsoft.com [216.32.180.30]) 8, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1NLJfFZ002234 8, 47 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 15:19:41 -0600 8, 47 -- Received: from mail211-va3-R.bigfish.com (10.7.14.225) by 8, 47 -- VA3EHSOBE012.bigfish.com (10.7.40.62) with Microsoft SMTP Server id 8, 47 -- 14.1.225.22; Sun, 23 Feb 2014 21:19:41 +0000 8, 47 -- Received: from mail211-va3 (localhost [127.0.0.1]) by 8, 47 -- mail211-va3-R.bigfish.com (Postfix) with ESMTP id 267679801EC for 8, 47 -- {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 21:19:41 +0000 (UTC) 8, 47 -- X-Forefront-Antispam-Report: CIP:164.107.81.212;KIP:(null);UIP:(null);IPV:NLI;H:cio-krc-pf05;RD:none;EFVD:NLI 8, 47 -- X-SpamScore: 2 8, 47 -- X-BigFish: VPS2(zzbb2dI62a3I98dI9371I1432Izz1f42h2148h1d77h208ch1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch1fc6hzd9hz1de098h177df4h17326ah8275bh1de097h186068hz2fh2a8h839h947hd25he5bhf0ah1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh162dh1631h1758h1765h18e1h190ch1946h19b4h19c3h19ceh1ad9h1b0ah224fh1d0ch1d2eh1d3fh1dfeh1dffh1f5fh1fe8h1ff5h209eh21e4h22d0h2336h2438h2461h2476h2487h24d7h2516h2545h255eh1b1cn1b1bi1155h) 8, 47 -- Received-SPF: pass (mail211-va3: domain of osu.edu designates 164.107.81.212 as permitted sender) client-ip=164.107.81.212; envelope-from=colijn.1-at-osu.edu; helo=cio-krc-pf05 ;cio-krc-pf05 ; 8, 47 -- Received: from mail211-va3 (localhost.localdomain [127.0.0.1]) by mail211-va3 8, 47 -- (MessageSwitch) id 1393190378901058_23421; Sun, 23 Feb 2014 21:19:38 +0000 8, 47 -- (UTC) 8, 47 -- Received: from VA3EHSMHS031.bigfish.com (unknown [10.7.14.226]) by 8, 47 -- mail211-va3.bigfish.com (Postfix) with ESMTP id CD13D80064; Sun, 23 Feb 2014 8, 47 -- 21:19:38 +0000 (UTC) 8, 47 -- Received: from cio-krc-pf05 (164.107.81.212) by VA3EHSMHS031.bigfish.com 8, 47 -- (10.7.99.41) with Microsoft SMTP Server (TLS) id 14.16.227.3; Sun, 23 Feb 8, 47 -- 2014 21:19:33 +0000 8, 47 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) (using TLSv1 8, 47 -- with cipher RC4-MD5 (128/128 bits)) (No client certificate requested) by 8, 47 -- cio-krc-pf05 (Postfix) with ESMTPS id F0DEA60056; Sun, 23 Feb 2014 16:19:32 8, 47 -- -0500 (EST) 8, 47 -- Received: from [192.168.1.102] (75.118.135.65) by smtp.service.osu.edu 8, 47 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.174.1; Sun, 23 Feb 8, 47 -- 2014 16:19:32 -0500 8, 47 -- Message-ID: {530A65E0.7020808-at-osu.edu} 8, 47 -- Date: Sun, 23 Feb 2014 16:19:28 -0500 8, 47 -- From: Henk Colijn {colijn.1-at-osu.edu} 8, 47 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 8, 47 -- MIME-Version: 1.0 8, 47 -- To: {microscopy-at-microscopy.com} , {jcsmtf-at-mail.missouri.edu} 8, 47 -- Subject: Re: [Microscopy] viaWWW:CM10 pneumatics 8, 47 -- References: {201402231459.s1NExkMp016345-at-ns.microscopy.com} 8, 47 -- In-Reply-To: {201402231459.s1NExkMp016345-at-ns.microscopy.com} 8, 47 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 8, 47 -- Content-Transfer-Encoding: 7bit 8, 47 -- X-Originating-IP: [75.118.135.65] 8, 47 -- X-CFilter-Loop: Forward 8, 47 -- X-FOPE-CRA-Verdict: 164.107.81.212$mail.missouri.edu%0%1%osu.edu%False%False%0$ 8, 47 -- X-OriginatorOrg: osu.edu 8, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 8, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%MAIL.MISSOURI.EDU$RO%1$TLS%0$FQDN%$TlsDn% ==============================End of - Headers==============================
The microscope air supply should *NOT* be attached to V11. V11 is the microscope vent valve and is used to release the vacuum in the column and camera chamber. It should never have more than a fraction of a psi supplied to it. Higher pressure can force the viewing chamber window out with great force and cause serious injury to anyone nearby! Venting the column with high pressure air when the sample rod is inserted can also blow the sample rod out with some force and damage it.
The air for the valves is supplied through the "Watts" regulator to the distribution block on the rear of the microscope column. This air ACTIVATES the valves; it doesn't pass through the valve.
Cheers, Henk
On 2/23/2014 9:59 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: jcsmtf-at-mail.missouri.edu () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both jcsmtf-at-mail.missouri.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: jcsmtf-at-mail.missouri.edu } Name: Josh Schorp } } Organization: University of Missouri } } Title-Subject: [Filtered] CM10 pneumatics } } Message: I am receiving a Pneumatics message on the screen of our CM10 and I believe this to be the } reason while none of the vacuums will start. The air line on our machine runs first to V11, but it } has not been allowed past V11. Is there a reason for this? I'm not sure how this valve works, I took } it off and it seems to be just a metal unit with no moving parts. I'm guessing the black electrical } piece that connects to it has something to do with its operations. Does anyone have any insight on } how to open this valve? I am running ~90 psi to it. } } Login Host: 161.130.188.19 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } ---===[|]===--- } } }
==============================Original Headers============================== 8, 47 -- From colijn.1-at-osu.edu Sun Feb 23 15:19:52 2014 8, 47 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1ehsobe006.messaging.microsoft.com [216.32.181.186]) 8, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1NLJqrO002523 8, 47 -- for {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 15:19:52 -0600 8, 47 -- Received: from mail151-ch1-R.bigfish.com (10.43.68.251) by 8, 47 -- CH1EHSOBE021.bigfish.com (10.43.70.78) with Microsoft SMTP Server id 8, 47 -- 14.1.225.22; Sun, 23 Feb 2014 21:19:52 +0000 8, 47 -- Received: from mail151-ch1 (localhost [127.0.0.1]) by 8, 47 -- mail151-ch1-R.bigfish.com (Postfix) with ESMTP id D9A711A036E for 8, 47 -- {microscopy-at-microscopy.com} ; Sun, 23 Feb 2014 21:19:51 +0000 (UTC) 8, 47 -- X-Forefront-Antispam-Report: CIP:164.107.81.218;KIP:(null);UIP:(null);IPV:NLI;H:cio-tnc-pf04;RD:none;EFVD:NLI 8, 47 -- X-SpamScore: 2 8, 47 -- X-BigFish: VPS2(zzbb2dI62a3I98dI9371I1432Izz1f42h2148h1d77h208ch1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch1fc6hzd9hz1de098h177df4h17326ah8275bh1de097h186068hz2fh2a8h839h947hd25he5bhf0ah1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh162dh1631h1758h1765h18e1h190ch1946h19b4h19c3h19ceh1ad9h1b0ah224fh1d0ch1d2eh1d3fh1dfeh1dffh1f5fh1fe8h1ff5h209eh22d0h2336h2438h2461h2476h2487h24d7h2516h2545h255eh1b1cn1b1bi1155h) 8, 47 -- Received-SPF: pass (mail151-ch1: domain of osu.edu designates 164.107.81.218 as permitted sender) client-ip=164.107.81.218; envelope-from=colijn.1-at-osu.edu; helo=cio-tnc-pf04 ;cio-tnc-pf04 ; 8, 47 -- Received: from mail151-ch1 (localhost.localdomain [127.0.0.1]) by mail151-ch1 8, 47 -- (MessageSwitch) id 1393190390194971_11380; Sun, 23 Feb 2014 21:19:50 +0000 8, 47 -- (UTC) 8, 47 -- Received: from CH1EHSMHS019.bigfish.com (snatpool3.int.messaging.microsoft.com 8, 47 -- [10.43.68.229]) by mail151-ch1.bigfish.com (Postfix) with ESMTP id 8, 47 -- 16C744004F; Sun, 23 Feb 2014 21:19:50 +0000 (UTC) 8, 47 -- Received: from cio-tnc-pf04 (164.107.81.218) by CH1EHSMHS019.bigfish.com 8, 47 -- (10.43.70.19) with Microsoft SMTP Server (TLS) id 14.16.227.3; Sun, 23 Feb 8, 47 -- 2014 21:19:47 +0000 8, 47 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) (using TLSv1 8, 47 -- with cipher RC4-MD5 (128/128 bits)) (No client certificate requested) by 8, 47 -- cio-tnc-pf04 (Postfix) with ESMTPS id D0190380057; Sun, 23 Feb 2014 16:19:46 8, 47 -- -0500 (EST) 8, 47 -- Received: from [192.168.1.102] (75.118.135.65) by smtp.service.osu.edu 8, 47 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.174.1; Sun, 23 Feb 8, 47 -- 2014 16:19:46 -0500 8, 47 -- Message-ID: {530A65ED.6030007-at-osu.edu} 8, 47 -- Date: Sun, 23 Feb 2014 16:19:41 -0500 8, 47 -- From: Henk Colijn {colijn.1-at-osu.edu} 8, 47 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 8, 47 -- MIME-Version: 1.0 8, 47 -- To: {microscopy-at-microscopy.com} , {jcsmtf-at-mail.missouri.edu} 8, 47 -- Subject: Re: [Microscopy] viaWWW:CM10 pneumatics 8, 47 -- References: {201402231459.s1NExkMp016345-at-ns.microscopy.com} 8, 47 -- In-Reply-To: {201402231459.s1NExkMp016345-at-ns.microscopy.com} 8, 47 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 8, 47 -- Content-Transfer-Encoding: 7bit 8, 47 -- X-Originating-IP: [75.118.135.65] 8, 47 -- X-CFilter-Loop: Forward 8, 47 -- X-FOPE-CRA-Verdict: 164.107.81.218$mail.missouri.edu%0%1%osu.edu%False%False%0$ 8, 47 -- X-OriginatorOrg: osu.edu 8, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 8, 47 -- X-FOPE-CONNECTOR: Id%0$Dn%MAIL.MISSOURI.EDU$RO%1$TLS%0$FQDN%$TlsDn% ==============================End of - Headers==============================
We have a very old SEM XL20 (FEI, runs under Windows 3.11, engl. version). Our hard disk crashed and we don't have a backup.
We have replaced the hard disk and got MS DOS and Win 3.11. installed, but we have problems with the operating system for the SEM. FEI is not able to help us, because the XL20 is so old.
Thus we are looking for: - a backup of the XL20 operating system or - XL20 - software V 5.24 (on discs or CD-ROM) including driver software or - EZ-SCSI-Driver (v3). (ADAPTEC-SCSI-SOFTWARE) or - MACH 64- Driver or - ATI-Driver for Win 3.11 (engl. Version).
Sincerely, Elisabeth Liebler-Tenorio
==============================Original Headers============================== 9, 29 -- From Elisabeth.Liebler-Tenorio-at-fli.bund.de Wed Feb 26 04:57:35 2014 9, 29 -- Received: from mx022.fli.bund.de (mx022.fli.bund.de [193.22.112.22]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QAvYMK027973 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Feb 2014 04:57:34 -0600 9, 29 -- Received: from FLI-RIE-HT039.intern.fli.bund.local (Not Verified[172.20.0.39]) by mx022.fli.bund.de with MailMarshal (v7,2,2,6606) 9, 29 -- id {B530dc8900000} ; Wed, 26 Feb 2014 11:57:20 +0100 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local (172.18.0.31) by 9, 29 -- FLI-RIE-HT039.intern.fli.bund.local (172.20.0.39) with Microsoft SMTP Server 9, 29 -- (TLS) id 14.3.174.1; Wed, 26 Feb 2014 11:57:34 +0100 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20]) 9, 29 -- by FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20%10]) with 9, 29 -- mapi id 14.03.0174.001; Wed, 26 Feb 2014 11:57:33 +0100 9, 29 -- From: "Liebler-Tenorio, Elisabeth" {Elisabeth.Liebler-Tenorio-at-fli.bund.de} 9, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 9, 29 -- CC: "ethalmann-at-t-online.de" {ethalmann-at-t-online.de} 9, 29 -- Subject: SEM XL20 (FEI) operating system/drivers 9, 29 -- Thread-Topic: SEM XL20 (FEI) operating system/drivers 9, 29 -- Thread-Index: Ac8y4WJQgviqvi0tQz6N3f8e25e20g== 9, 29 -- Date: Wed, 26 Feb 2014 10:57:32 +0000 9, 29 -- Message-ID: {6936FD3A0444524A8FA0A7FB2BBAFDB40C3EBCCA-at-FLI-JE-MB031.intern.fli.bund.local} 9, 29 -- Accept-Language: de-DE, en-US 9, 29 -- Content-Language: de-DE 9, 29 -- X-MS-Has-Attach: 9, 29 -- X-MS-TNEF-Correlator: 9, 29 -- x-originating-ip: [172.18.2.80] 9, 29 -- Content-Type: text/plain; charset="iso-8859-1" 9, 29 -- MIME-Version: 1.0 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1QAvYMK027973 ==============================End of - Headers==============================
Dear Elisabeth, I found some old diskettes with following ATI MACH-64 drivers for Win3.11:
ATI Mach-64_2012 ATI Mach-64_222 ATI Mach-64_303
If you like, I can send you an archiv (zip) with all the files I have found on them.
Best regards from Prague
Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Wed, 26 Feb 2014 05:02:18 -0600, Elisabeth.Liebler-Tenorio-at-fli.bund.de wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear members of the listserver: } } We have a very old SEM XL20 (FEI, runs under Windows 3.11, engl. } version). Our hard disk crashed and we don't have a backup. } } We have replaced the hard disk and got MS DOS and Win 3.11. } installed, but we have problems with the operating system for the } SEM. FEI is not able to help us, because the XL20 is so old. } } Thus we are looking for: } - a backup of the XL20 operating system or } -Â XL20 - software V 5.24 (on discs or CD-ROM) including driver } software or } -Â EZ-SCSI-Driver (v3). (ADAPTEC-SCSI-SOFTWARE) or } -Â MACH 64- Driver or } -Â ATI-Driver for Win 3.11 (engl. Version). } } Sincerely, Elisabeth Liebler-Tenorio } } } } } } ==============================Original } Headers============================== 9, 29 -- From } Elisabeth.Liebler-Tenorio-at-fli.bund.de Wed Feb 26 04:57:35 2014 9, 29 } -- Received: from mx022.fli.bund.de (mx022.fli.bund.de } [193.22.112.22]) 9, 29 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id s1QAvYMK027973 9, 29 -- } for {Microscopy-at-Microscopy.Com} ; Wed, 26 Feb 2014 04:57:34 } -0600 9, 29 -- Received: from FLI-RIE-HT039.intern.fli.bund.local } (Not Verified[172.20.0.39]) by mx022.fli.bund.de with MailMarshal } (v7,2,2,6606) 9, 29 -- id {B530dc8900000} ; Wed, 26 Feb 2014 } 11:57:20 +0100 9, 29 -- Received: from } FLI-JE-MB031.intern.fli.bund.local (172.18.0.31) by 9, 29 -- } FLI-RIE-HT039.intern.fli.bund.local (172.20.0.39) with Microsoft SMTP } Server 9, 29 -- (TLS) id 14.3.174.1; Wed, 26 Feb 2014 11:57:34 +0100 } 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local } ([fe80::9167:29a:d9b4:9c20]) 9, 29 -- by } FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20%10]) } with 9, 29 -- mapi id 14.03.0174.001; Wed, 26 Feb 2014 11:57:33 } +0100 9, 29 -- From: "Liebler-Tenorio, Elisabeth" } {Elisabeth.Liebler-Tenorio-at-fli.bund.de} 9, 29 -- To: } "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 9, 29 -- CC: } "ethalmann-at-t-online.de" {ethalmann-at-t-online.de} 9, 29 -- Subject: SEM } XL20 (FEI) operating system/drivers 9, 29 -- Thread-Topic: SEM XL20 } (FEI) operating system/drivers 9, 29 -- Thread-Index: } Ac8y4WJQgviqvi0tQz6N3f8e25e20g== 9, 29 -- Date: Wed, 26 Feb 2014 } 10:57:32 +0000 9, 29 -- Message-ID: } {6936FD3A0444524A8FA0A7FB2BBAFDB40C3EBCCA-at-FLI-JE-MB031.intern.fli.bund.local} } 9, 29 -- Accept-Language: de-DE, en-US 9, 29 -- Content-Language: } de-DE 9, 29 -- X-MS-Has-Attach: 9, 29 -- X-MS-TNEF-Correlator: 9, 29 } -- x-originating-ip: [172.18.2.80] 9, 29 -- Content-Type: text/plain; } charset="iso-8859-1" 9, 29 -- MIME-Version: 1.0 9, 29 -- } Content-Transfer-Encoding: 8bit 9, 29 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id s1QAvYMK027973 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 40 -- From benada-at-biomed.cas.cz Wed Feb 26 08:09:01 2014 8, 40 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QE90Ln019616 8, 40 -- for {Microscopy-at-Microscopy.com} ; Wed, 26 Feb 2014 08:09:01 -0600 8, 40 -- X-ASG-Debug-ID: 1393423738-05011e721bd0290001-FOsErg 8, 40 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id SlsMxgyA6FQszjI5 (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO); Wed, 26 Feb 2014 15:08:58 +0100 (CET) 8, 40 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 8, 40 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 8, 40 -- Received: from u117fs (c111kh.mbu.cas.cz [147.231.44.14]) 8, 40 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 8, 40 -- (No client certificate requested) 8, 40 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 54B132C81A0; 8, 40 -- Wed, 26 Feb 2014 15:08:58 +0100 (CET) 8, 40 -- Date: Wed, 26 Feb 2014 15:08:56 +0100 8, 40 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 8, 40 -- To: Elisabeth.Liebler-Tenorio-at-fli.bund.de, Microscopy-at-Microscopy.com 8, 40 -- Subject: Re: [Microscopy] SEM XL20 (FEI) operating system/drivers 8, 40 -- Message-ID: {20140226150856.562b7ae3-at-u117fs} 8, 40 -- X-ASG-Orig-Subj: Re: [Microscopy] SEM XL20 (FEI) operating system/drivers 8, 40 -- In-Reply-To: {201402261102.s1QB2I7a030920-at-ns.microscopy.com} 8, 40 -- References: {201402261102.s1QB2I7a030920-at-ns.microscopy.com} 8, 40 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 8, 40 -- =?UTF-8?B?xIxS?= 8, 40 -- X-Mailer: Claws Mail 3.9.3 (GTK+ 2.24.10; i486-pc-linux-gnu) 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Type: text/plain; charset=UTF-8 8, 40 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 8, 40 -- X-Barracuda-Start-Time: 1393423738 8, 40 -- X-Barracuda-Encrypted: AES256-SHA 8, 40 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 8, 40 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 8, 40 -- X-Barracuda-BRTS-Status: 1 8, 40 -- X-Barracuda-Spam-Score: 0.00 8, 40 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 8, 40 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.145532 8, 40 -- Rule breakdown below 8, 40 -- pts rule name description 8, 40 -- ---- ---------------------- -------------------------------------------------- 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1QE90Ln019616 ==============================End of - Headers==============================
Last summer I had fun recovering WIN-NT PC on FEI FIB620 (Phillips XL30 SEM with ion beam column added) from a hard disk crash.
If your original disk is physically operational, then recovering SEM software from it may still be possible. Not sure if PC "hackery" is of interest for everyone, so please contact me off list you want/need to give it a try.
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/26/2014 5:59 AM, Elisabeth.Liebler-Tenorio-at-fli.bund.de wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear members of the listserver: } } We have a very old SEM XL20 (FEI, runs under Windows 3.11, engl. version). Our hard disk crashed and we don't have a backup. } } We have replaced the hard disk and got MS DOS and Win 3.11. installed, but we have problems with the operating system for the SEM. FEI is not able to help us, because the XL20 is so old. } } Thus we are looking for: } - a backup of the XL20 operating system or } - XL20 - software V 5.24 (on discs or CD-ROM) including driver software or } - EZ-SCSI-Driver (v3). (ADAPTEC-SCSI-SOFTWARE) or } - MACH 64- Driver or } - ATI-Driver for Win 3.11 (engl. Version). } } Sincerely, Elisabeth Liebler-Tenorio } } } } } } ==============================Original Headers============================== } 9, 29 -- From Elisabeth.Liebler-Tenorio-at-fli.bund.de Wed Feb 26 04:57:35 2014 } 9, 29 -- Received: from mx022.fli.bund.de (mx022.fli.bund.de [193.22.112.22]) } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QAvYMK027973 } 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Feb 2014 04:57:34 -0600 } 9, 29 -- Received: from FLI-RIE-HT039.intern.fli.bund.local (Not Verified[172.20.0.39]) by mx022.fli.bund.de with MailMarshal (v7,2,2,6606) } 9, 29 -- id {B530dc8900000} ; Wed, 26 Feb 2014 11:57:20 +0100 } 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local (172.18.0.31) by } 9, 29 -- FLI-RIE-HT039.intern.fli.bund.local (172.20.0.39) with Microsoft SMTP Server } 9, 29 -- (TLS) id 14.3.174.1; Wed, 26 Feb 2014 11:57:34 +0100 } 9, 29 -- Received: from FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20]) } 9, 29 -- by FLI-JE-MB031.intern.fli.bund.local ([fe80::9167:29a:d9b4:9c20%10]) with } 9, 29 -- mapi id 14.03.0174.001; Wed, 26 Feb 2014 11:57:33 +0100 } 9, 29 -- From: "Liebler-Tenorio, Elisabeth" {Elisabeth.Liebler-Tenorio-at-fli.bund.de} } 9, 29 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 9, 29 -- CC: "ethalmann-at-t-online.de" {ethalmann-at-t-online.de} } 9, 29 -- Subject: SEM XL20 (FEI) operating system/drivers } 9, 29 -- Thread-Topic: SEM XL20 (FEI) operating system/drivers } 9, 29 -- Thread-Index: Ac8y4WJQgviqvi0tQz6N3f8e25e20g== } 9, 29 -- Date: Wed, 26 Feb 2014 10:57:32 +0000 } 9, 29 -- Message-ID: {6936FD3A0444524A8FA0A7FB2BBAFDB40C3EBCCA-at-FLI-JE-MB031.intern.fli.bund.local} } 9, 29 -- Accept-Language: de-DE, en-US } 9, 29 -- Content-Language: de-DE } 9, 29 -- X-MS-Has-Attach: } 9, 29 -- X-MS-TNEF-Correlator: } 9, 29 -- x-originating-ip: [172.18.2.80] } 9, 29 -- Content-Type: text/plain; charset="iso-8859-1" } 9, 29 -- MIME-Version: 1.0 } 9, 29 -- Content-Transfer-Encoding: 8bit } 9, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1QAvYMK027973 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 43 -- From vray-at-partbeamsystech.com Wed Feb 26 08:23:26 2014 5, 43 -- Received: from nm16-vm6.bullet.mail.ne1.yahoo.com (nm16-vm6.bullet.mail.ne1.yahoo.com [98.138.91.109]) 5, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QENPpK003558 5, 43 -- for {microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 08:23:25 -0600 5, 43 -- Received: from [98.138.101.130] by nm16.bullet.mail.ne1.yahoo.com with NNFMP; 26 Feb 2014 14:23:25 -0000 5, 43 -- Received: from [98.138.84.45] by tm18.bullet.mail.ne1.yahoo.com with NNFMP; 26 Feb 2014 14:23:25 -0000 5, 43 -- Received: from [127.0.0.1] by smtp113.mail.ne1.yahoo.com with NNFMP; 26 Feb 2014 14:23:25 -0000 5, 43 -- X-Yahoo-Newman-Id: 218200.74829.bm-at-smtp113.mail.ne1.yahoo.com 5, 43 -- X-Yahoo-Newman-Property: ymail-3 5, 43 -- X-YMail-OSG: NHu4eV8VM1l6KHm.QA.gbcMhOPEW.KAbNNGAIV_gcUOeBxM 5, 43 -- xmkAgqs8uy8nhJ8asaWu4V54p6Bzi1LyhZlC.E5DXfyD4TYn56kYvvYZa7_w 5, 43 -- FIfeaieqFCuSG6Rbc0O5NOdwyCKzeMVtH9TqN7Hz5WK7Z6w6pXrSV1LWAvrY 5, 43 -- L4PktQFlYXq2_OMevjr3s3A_Nv.7UX6qfro_j_A44iBPsXxKEYiWeDdPLQiK 5, 43 -- ep7gRgeAQGa2whGjs_mOrFyrvYt_LJlwm3oGpxOjm_QQHC3Ox9He5xvAjpfT 5, 43 -- PLqQ4Rsau6ZXlc3Imra3HNwGgdMcYiDvBucCC3oclwcOqMkqpuyL4EXp5wVF 5, 43 -- NZdmCPhFg8OW5kcivz6YFcivvfPBDgqy3_Xdtt0Nvpz7QafSmPVyIClbp.EV 5, 43 -- 26.Ccgw8giBEt79_ydhOk3fbhPbNVqpOoQ_L5R4dIIVLzqi_74QfnCKYGwvz 5, 43 -- 4JHd8cG3928ULJfWvyosDJOgEiLLmGNV43zafiwVKjY7PzU1ICQgbXyOsOw9 5, 43 -- mC5gKRHCWEE.M3Xx8RBq7LMMQaVbH0MzE_1UFudEmYF4ZdLYxf6x4ZTQR4im 5, 43 -- ceFmmYoDb7qX2V.YWYQK42DaVQKwr.v3OrS3UK0mWbw7KYP9QZfShz7nG8qf 5, 43 -- 5oYefWkxVN2SAfER._eY.Ad8j63uhQP3gKD2wCC81GJCERc6fL9HwHognAkS 5, 43 -- 0ZjHMsAGCk_oCf92vpzo7qr6aTZtmAcdlosXG3jh0_YwhC2Srsa4XxJkxmad 5, 43 -- Zjrk2_i1VJrk5Lx4dMgIllyIUCd7_qfOMAHMMtuv0VJ2PBKPuKAQzkfTwS8T 5, 43 -- actcyPOTn2wvtPgh6O.FXRl4s.CQxHtxjVWB43WQ8_.epMaJl_pkQ9tx3yvE 5, 43 -- BLHuwE4Vnc5aqEDPl1FAnkRLdw7XC8Dn9lBdZ77ALBQZ08vwoykrcQ3p7CZn 5, 43 -- SNi6BH8TrhDg- 5, 43 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 43 -- X-Rocket-Received: from [192.168.15.134] (vray-at-75.93.147.105 with plain [98.139.211.125]) 5, 43 -- by smtp113.mail.ne1.yahoo.com with SMTP; 26 Feb 2014 06:23:25 -0800 PST 5, 43 -- Message-ID: {530DF8DB.6050206-at-partbeamsystech.com} 5, 43 -- Date: Wed, 26 Feb 2014 09:23:23 -0500 5, 43 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 5, 43 -- Reply-To: vray-at-partbeamsystech.com 5, 43 -- Organization: PBS&T 5, 43 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 5, 43 -- MIME-Version: 1.0 5, 43 -- To: Elisabeth.Liebler-Tenorio-at-fli.bund.de 5, 43 -- CC: microscopy-at-microscopy.com 5, 43 -- Subject: Re: [Microscopy] SEM XL20 (FEI) operating system/drivers 5, 43 -- References: {201402261059.s1QAx9uw029163-at-ns.microscopy.com} 5, 43 -- In-Reply-To: {201402261059.s1QAx9uw029163-at-ns.microscopy.com} 5, 43 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 43 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A user of our JEOL 2100 TEM informed me that he was noticing anomalous rings appearing in diffraction patterns acquired with the SAD aperture centered over vacuum (he's developing a quantitative diffraction technique and presumably is acquiring a background diffraction spectrum). I triple checked the microscope alignment in TEM mode, STEM, nanobeam diffraction, convergent beam diffraction, and the high current EDS mode are all well aligned over the full range of spot sizes and convergence angles. The lens voltages and alignment coils all fall within their suitable ranges as well. When I acquired diffraction patterns of actual sample I don't see the artifacts in the DP, presumably because the intensity of the actual diffraction rings/spots washes out the weak artifact rings.
I checked all the condensor and selected area apertures, and all exhibit the problem as far as I can tell. I also triple checked to ensure all other apertures were removed and not displaced into a wrong position by a user, and they all checked out as well.
I'm attaching a link to two images of the artifact. These were taken at 200kV but the same is seen at 120kV (and probably 80kV--have not checked). You can see that there are two rings and their size and spacing change with changing camera lengths. Is this caused by diffraction of something in the beam path--either an aperture carrier or lost sample--or another effect, or am I missing something simple? Thanks for any hints or help directed my way.
Link to DPs showing artifacts: http://imgur.com/a/xCV4R
Thanks, Chris Virginia Tech
==============================Original Headers============================== 6, 26 -- From microwink-at-gmail.com Wed Feb 26 17:23:29 2014 6, 26 -- Received: from mail-yh0-f44.google.com (mail-yh0-f44.google.com [209.85.213.44]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QNNSl2004342 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 17:23:28 -0600 6, 26 -- Received: by mail-yh0-f44.google.com with SMTP id f73so1872230yha.17 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 15:23:28 -0800 (PST) 6, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=20120113; 6, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 6, 26 -- bh=YnUClmt1SX7+iNo6a5C5nRvjVCMrdlTnLTwfTKWxnMI=; 6, 26 -- b=UWHMLVjuyASU2VIeWj9DTIqV7UpS3YgnVDMF5b3MM3UaWdNstB6mGRufrEin2TO56S 6, 26 -- cp6qZ0bQKggJ1XvulYzyMxOg9tjsxPWWqsmxqP3f2WdasGp8vltqHmH4E70elKoSFuzW 6, 26 -- J7gyq8tUTk0NzuJh86xgG819RMXRdWPdyYr8DbCoDv655Nz5tSAIBiu/XOOdLerV+4Iw 6, 26 -- FzdhgzNu6euCnn4Ly2hj+mmJBPm32U33lZE5DN9OPOQnRvtyhQF32o9CyCsa+osenzsc 6, 26 -- yWmX/XucvhB2Z2ZbY5mLUXkLCEGQOR6oFR6XkYFKG+V1yZHQUgInl77IsKfQVkzl/QC0 6, 26 -- CvNA== 6, 26 -- MIME-Version: 1.0 6, 26 -- X-Received: by 10.236.152.36 with SMTP id c24mr5737180yhk.118.1393457008189; 6, 26 -- Wed, 26 Feb 2014 15:23:28 -0800 (PST) 6, 26 -- Received: by 10.170.180.8 with HTTP; Wed, 26 Feb 2014 15:23:28 -0800 (PST) 6, 26 -- Date: Wed, 26 Feb 2014 18:23:28 -0500 6, 26 -- Message-ID: {CAA8T2PO4yOMNE7O7XY=H-AzOvCq3H7pAQNOE=oUq4F_0TvrGRg-at-mail.gmail.com} 6, 26 -- Subject: Anomalous diffraction rings in JEOL 2100 6, 26 -- From: Christopher Winkler {microwink-at-gmail.com} 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
On Feb 26, 2014, at 3:34 PM, microwink-at-gmail.com wrote:
} A user of our JEOL 2100 TEM informed me that he was noticing anomalous } rings appearing in diffraction patterns acquired with the SAD aperture } centered over vacuum (he's developing a quantitative diffraction } technique and presumably is acquiring a background diffraction } spectrum). I triple checked the microscope alignment in TEM mode, } STEM, nanobeam diffraction, convergent beam diffraction, and the high } current EDS mode are all well aligned over the full range of spot } sizes and convergence angles. The lens voltages and alignment coils } all fall within their suitable ranges as well. When I acquired } diffraction patterns of actual sample I don't see the artifacts in the } DP, presumably because the intensity of the actual diffraction } rings/spots washes out the weak artifact rings. } } I checked all the condensor and selected area apertures, and all } exhibit the problem as far as I can tell. I also triple checked to } ensure all other apertures were removed and not displaced into a wrong } position by a user, and they all checked out as well. } } I'm attaching a link to two images of the artifact. These were taken } at 200kV but the same is seen at 120kV (and probably 80kV--have not } checked). You can see that there are two rings and their size and } spacing change with changing camera lengths. Is this caused by } diffraction of something in the beam path--either an aperture carrier } or lost sample--or another effect, or am I missing something simple? } Thanks for any hints or help directed my way. } } Link to DPs showing artifacts: http://imgur.com/a/xCV4R } } Thanks, } Chris } Virginia Tech } Dear Chris, I noticed that the rings are not centered with the undiffracted beam. Also, the pattern appears to be from a polycrystalline specimen. It may be that the beam emitted by the FEG/filament is split in two--either by having two points emitting from the source or by the beam hitting something near the top of the column producing a scattered beam. If this second beam strikes some polycrystalline substance, such as a grid near the point of vacuum where the main beam is directed, it could produce something like the DPs on the web site. Do the DPs change when the stage is moved (while keeping the beam over vacuum, of course)? Yours, Bill
==============================Original Headers============================== 6, 37 -- From wtivol-at-sbcglobal.net Wed Feb 26 20:49:06 2014 6, 37 -- Received: from nm4.access.bullet.mail.gq1.yahoo.com (nm4.access.bullet.mail.gq1.yahoo.com [216.39.62.35]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1R2n5rD026815 6, 37 -- for {microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 20:49:06 -0600 6, 37 -- Received: from [216.39.60.165] by nm4.access.bullet.mail.gq1.yahoo.com with NNFMP; 27 Feb 2014 02:49:05 -0000 6, 37 -- Received: from [67.195.23.146] by tm1.access.bullet.mail.gq1.yahoo.com with NNFMP; 27 Feb 2014 02:49:05 -0000 6, 37 -- Received: from [127.0.0.1] by smtp118.sbc.mail.gq1.yahoo.com with NNFMP; 27 Feb 2014 02:49:05 -0000 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s1024; t=1393469345; bh=NAfGz1+z1PWb307Um5ez/cLfUfFZ7ws6BHtFsoWd50A=; h=X-Yahoo-Newman-Id:X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:Cc:Message-Id:From:To:In-Reply-To:Content-Type:Content-Transfer-Encoding:Mime-Version:Subject:Date:References:X-Mailer; b=RWn/yyOh6s8T0ptuPxh1nK5RzKl3XIphkJv0cKEgPwOHDUoueT7i9+lPYDh/0qjaWmxyapMNSdKbQY3HavPLSYW6Twk9cb8VnwS1AuAMZ4rJ8VJlERUzRiVzvWBYqG/n420yoH0dUveskPTkBmTcxz1H0SoYTTSI+WbnCKiGYIs= 6, 37 -- X-Yahoo-Newman-Id: 53491.57855.bm-at-smtp118.sbc.mail.gq1.yahoo.com 6, 37 -- X-Yahoo-Newman-Property: ymail-3 6, 37 -- X-YMail-OSG: mi394ygVM1mlzvwPWYKq5q.g5AzZ6Cqyu_R_CgGDJ.kLL4q 6, 37 -- bKG0rR83LENITjBnNpkqj8YOT8Kp.b3H4yrAv9DQPGhmnnCrSXCTDsJmeZbb 6, 37 -- cxxu40qv9KV7r1d1esr_n8PNOU_9xJLmtpr09NuuXNjp2uKaWwSb7Yg6mK_B 6, 37 -- bugBTvx.qUWBUYBLiFYKZVjvTlKzwg1bYxrxbuo4KCDH3ubyKfgpLLHxgavf 6, 37 -- bnvRLsHkwwGVphK51llvC2v8hyT1wAWZ6d74VKD7drLtDAcKOzxfYaKZQ.QR 6, 37 -- pkPjvYt1NefwdaUrj3tTLr3k.KN4pGV376jt3.JPaNhkkJ1.Zx6UycHa8Vl5 6, 37 -- RJj.iG4JwdVxeLDDqmrK1xqoBqX88AGlk83DxXnfAkGBbEzNCGsqcDHo2AFP 6, 37 -- l5ux1lp_aAC0du1XyAyp.SIEru4tIk.1jRRjc7U4VrgB9Ij_DdphYUF.6JrL 6, 37 -- _ijfERFssFZ8z7PveK8LtyyHdDylFvrvywsdCniw6oIIWZsp95juP4XCzBBx 6, 37 -- 5S2D1oyBzrdx2gVUh3jCaLpprw.yLQ_QrFrky8wU9kVCwJPFRoicde2uviIY 6, 37 -- C13KK1bJK5C8NXtt8l38sGAuZ33h6otiu7QFBT2lq68kEKNEuTNrCR.lYvPf 6, 37 -- 2pNcguzOgqeXLqA-- 6, 37 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 37 -- X-Rocket-Received: from [192.168.1.68] (wtivol-at-108.226.244.17 with plain [67.195.15.66]) 6, 37 -- by smtp118.sbc.mail.gq1.yahoo.com with SMTP; 27 Feb 2014 02:49:05 +0000 UTC 6, 37 -- Cc: microscopy-at-microscopy.com 6, 37 -- Message-Id: {77CAAC6F-3B04-4011-9597-B2C9840AC9C2-at-sbcglobal.net} 6, 37 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 37 -- To: microwink-at-gmail.com 6, 37 -- In-Reply-To: {201402262334.s1QNYjkh019441-at-ns.microscopy.com} 6, 37 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 37 -- Content-Transfer-Encoding: 7bit 6, 37 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 37 -- Subject: Re: [Microscopy] Anomalous diffraction rings in JEOL 2100 6, 37 -- Date: Wed, 26 Feb 2014 18:49:03 -0800 6, 37 -- References: {201402262334.s1QNYjkh019441-at-ns.microscopy.com} 6, 37 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Yes, I used to see these on our JEOL 2500 when I was at HP. Tomba San, Mr Tomba at JEOL fixed it by always having us do diffraction left of crossover- where the beam is more parallel! That will most likely give the heave ho to your rings!
Ganbette Good Luck or Hang in there!
Pete Eschbach Oregon State EM Facility
Sent from my iPhone
} On Feb 26, 2014, at 3:35 PM, microwink-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all, } } A user of our JEOL 2100 TEM informed me that he was noticing anomalous } rings appearing in diffraction patterns acquired with the SAD aperture } centered over vacuum (he's developing a quantitative diffraction } technique and presumably is acquiring a background diffraction } spectrum). I triple checked the microscope alignment in TEM mode, } STEM, nanobeam diffraction, convergent beam diffraction, and the high } current EDS mode are all well aligned over the full range of spot } sizes and convergence angles. The lens voltages and alignment coils } all fall within their suitable ranges as well. When I acquired } diffraction patterns of actual sample I don't see the artifacts in the } DP, presumably because the intensity of the actual diffraction } rings/spots washes out the weak artifact rings. } } I checked all the condensor and selected area apertures, and all } exhibit the problem as far as I can tell. I also triple checked to } ensure all other apertures were removed and not displaced into a wrong } position by a user, and they all checked out as well. } } I'm attaching a link to two images of the artifact. These were taken } at 200kV but the same is seen at 120kV (and probably 80kV--have not } checked). You can see that there are two rings and their size and } spacing change with changing camera lengths. Is this caused by } diffraction of something in the beam path--either an aperture carrier } or lost sample--or another effect, or am I missing something simple? } Thanks for any hints or help directed my way. } } Link to DPs showing artifacts: http://imgur.com/a/xCV4R } } Thanks, } Chris } Virginia Tech } } ==============================Original Headers============================== } 6, 26 -- From microwink-at-gmail.com Wed Feb 26 17:23:29 2014 } 6, 26 -- Received: from mail-yh0-f44.google.com (mail-yh0-f44.google.com [209.85.213.44]) } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1QNNSl2004342 } 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 17:23:28 -0600 } 6, 26 -- Received: by mail-yh0-f44.google.com with SMTP id f73so1872230yha.17 } 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Feb 2014 15:23:28 -0800 (PST) } 6, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 26 -- d=gmail.com; s=20120113; } 6, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; } 6, 26 -- bh=YnUClmt1SX7+iNo6a5C5nRvjVCMrdlTnLTwfTKWxnMI=; } 6, 26 -- b=UWHMLVjuyASU2VIeWj9DTIqV7UpS3YgnVDMF5b3MM3UaWdNstB6mGRufrEin2TO56S } 6, 26 -- cp6qZ0bQKggJ1XvulYzyMxOg9tjsxPWWqsmxqP3f2WdasGp8vltqHmH4E70elKoSFuzW } 6, 26 -- J7gyq8tUTk0NzuJh86xgG819RMXRdWPdyYr8DbCoDv655Nz5tSAIBiu/XOOdLerV+4Iw } 6, 26 -- FzdhgzNu6euCnn4Ly2hj+mmJBPm32U33lZE5DN9OPOQnRvtyhQF32o9CyCsa+osenzsc } 6, 26 -- yWmX/XucvhB2Z2ZbY5mLUXkLCEGQOR6oFR6XkYFKG+V1yZHQUgInl77IsKfQVkzl/QC0 } 6, 26 -- CvNA== } 6, 26 -- MIME-Version: 1.0 } 6, 26 -- X-Received: by 10.236.152.36 with SMTP id c24mr5737180yhk.118.1393457008189; } 6, 26 -- Wed, 26 Feb 2014 15:23:28 -0800 (PST) } 6, 26 -- Received: by 10.170.180.8 with HTTP; Wed, 26 Feb 2014 15:23:28 -0800 (PST) } 6, 26 -- Date: Wed, 26 Feb 2014 18:23:28 -0500 } 6, 26 -- Message-ID: {CAA8T2PO4yOMNE7O7XY=H-AzOvCq3H7pAQNOE=oUq4F_0TvrGRg-at-mail.gmail.com} } 6, 26 -- Subject: Anomalous diffraction rings in JEOL 2100 } 6, 26 -- From: Christopher Winkler {microwink-at-gmail.com} } 6, 26 -- To: Microscopy-at-microscopy.com } 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 35 -- From peter.eschbach-at-comcast.net Thu Feb 27 11:07:48 2014 7, 35 -- Received: from qmta08.emeryville.ca.mail.comcast.net (qmta08.emeryville.ca.mail.comcast.net [76.96.30.80]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1RH7lRS029103 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 27 Feb 2014 11:07:48 -0600 7, 35 -- Received: from omta23.emeryville.ca.mail.comcast.net ([76.96.30.90]) 7, 35 -- by qmta08.emeryville.ca.mail.comcast.net with comcast 7, 35 -- id XUNS1n0021wfjNsA8V7nsn; Thu, 27 Feb 2014 17:07:47 +0000 7, 35 -- Received: from [IPv6:2601:7:1400:944:d00a:c8f2:f8db:188a] ([IPv6:2601:7:1400:944:d00a:c8f2:f8db:188a]) 7, 35 -- by omta23.emeryville.ca.mail.comcast.net with comcast 7, 35 -- id XV7m1n00J3S7LFo8jV7mj1; Thu, 27 Feb 2014 17:07:47 +0000 7, 35 -- Subject: Re: [Microscopy] Anomalous diffraction rings in JEOL 2100 7, 35 -- References: {201402262335.s1QNZFrh020221-at-ns.microscopy.com} 7, 35 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 7, 35 -- Content-Type: text/plain; 7, 35 -- charset=us-ascii 7, 35 -- X-Mailer: iPhone Mail (11B554a) 7, 35 -- In-Reply-To: {201402262335.s1QNZFrh020221-at-ns.microscopy.com} 7, 35 -- Message-Id: {E1A91431-90BC-4DBD-899C-62A1F838DA57-at-comcast.net} 7, 35 -- Date: Thu, 27 Feb 2014 09:07:47 -0800 7, 35 -- To: "microwink-at-gmail.com" {microwink-at-gmail.com} , 7, 35 -- "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 7, 35 -- Mime-Version: 1.0 (1.0) 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 7, 35 -- s=q20121106; t=1393520867; 7, 35 -- bh=CnWCEsaPwyXIAT4u9IhI5qHRfFttXjLbgwhlKMuumXI=; 7, 35 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: 7, 35 -- Mime-Version; 7, 35 -- b=NSFq4OMr77tuu9gqm9RByys3+fUESSO77yDBaf5u09++SR3j9ZQWh+ib/wUqsSenE 7, 35 -- vUhWEjsp9JaRQgeFgM105zZ27tqn+TZz8fBW7F9mgmAIS8i55ef57AhDeIcj8vkVBe 7, 35 -- nnXm61KRqQ50vDqylI1LU/+RXN9yJZKLlUQuv7nyBbh0mY3EbT+qzloB07PLtF6Bg0 7, 35 -- D8hFh9fD0JoUBGq0xjeV+R+tZNcghIeViB/5Iewp/8aD2hhtpoUUZOEjEFP9l7qNiz 7, 35 -- Ed4j8TjP7Nu56EETAYqVpne0vbzMCscgs6Tcr8teN4G3XgqZZ1ZCngPVCT6KNiClgM 7, 35 -- 0uLFrIcV2HFkw== 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1RH7lRS029103 ==============================End of - Headers==============================
The deadline for abstract submission has passed and as you are making plans to attend the Hartford meetings Aug. 3-7, please don*t forget about MSA*s student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs; giving them an opportunity to meet and interact with the established microscopy community.
The students will be paid $10 an hour to work for ~20 hours (up to 40 hours) during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia (assisting with audio-visual needs, speaker set-up, maintaining an attendance count), staffing the volunteer office, monitoring use of the Internet Café, and helping with vendor tutorial sign-up. Payment is given as a check at the end of the meetings or when the student leaves Hartford.
Once the program has been finalized, each registered bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up *working* sessions they would attend anyway. There is an added bonus of $10 cash to help with meals for each morning and/or afternoon session worked and a meeting shirt.
If anyone would like to participate in the bursary program, please check the *I wish to apply for a student bursary* box in section 2 of the registration form and send me an email of your intention. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.
For those *non-students* volunteers are also needed to help with the above mentioned meeting activities. Although not paid on an hourly basis as the student bursaries, volunteers do receive a meeting shirt and the same cash allotment to help with meals. Volunteers also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact:
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-7998 alawrence-at-i2at.msstate.edu
==============================Original Headers============================== 11, 23 -- From ALawrence-at-i2at.msstate.edu Thu Feb 27 14:28:21 2014 11, 23 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1RKSLes019434 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 27 Feb 2014 14:28:21 -0600 11, 23 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197]) 11, 23 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id s1RKSLSn018820 11, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 27 Feb 2014 14:28:21 -0600 11, 23 -- X-Sender: {} 11, 23 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu 11, 23 -- with Novell_GroupWise; Thu, 27 Feb 2014 14:28:21 -0600 11, 23 -- Message-Id: {530F4B83020000E6000DA098-at-mailhost.groupwise.msstate.edu} 11, 23 -- X-Mailer: Novell GroupWise Internet Agent 12.0.2 11, 23 -- Date: Thu, 27 Feb 2014 14:28:19 -0600 11, 23 -- From: "Amanda Lawrence" {ALawrence-at-i2at.msstate.edu} 11, 23 -- To: {microscopy-at-microscopy.com} 11, 23 -- Subject: M&M 2014 Student Bursaries and Volunteers 11, 23 -- References: {530F3EE6020000E6000DA088-at-mailhost.groupwise.msstate.edu} 11, 23 -- {530F4B83020000E6000DA098-at-mailhost.groupwise.msstate.edu} 11, 23 -- Mime-Version: 1.0 11, 23 -- Content-Type: text/plain; charset=ISO-8859-15 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- Content-Disposition: inline ==============================End of - Headers==============================
X-from: lamiller-at-illinois.edu () To: Zaluzec-at-MICROSCOPY.COM
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Email: lamiller-at-illinois.edu Name: Lou Ann Miller
Organization: Materials Research Lab, U of Illinois
Title-Subject: [Filtered] MRL Biological microscopy and analysis work shop Opportunity for vendors, and for students
Message: Hello!
Many thanks to many of you for helping out with our fall biological conference in 2013!
This year, we hope to have the conference November 12 & 13th, 2014.
Students: There will be a poster and presentation Competition at this year's MRL Biological microscopy and analysis work shop.
Each year we have had a different theme, 1st Biostructures and "what are we looking at", last year Techniques: " how do we do that?"
This year, I'm hoping to pull in more students and participation by having a student presentation and poster competition as well as talks by those who have specialties here on campus.. The subject will be how students put biological microscopy techniques into use.
VENDORS:
We would like to offer prizes for the best presentations with posters. Of course this is where you can come in. :-)
Because you are vendors with the university, we checked all the rules, and it appears that a check from a vendor straight to the prize winner can be up to , but not exceed $100.
The first 3 vendors, who can offer a nice monetary prize($100 or less), will get to be on the judges panel, as well as have the prize named after your company. We can send the results with images with your company's name to our paper, and to the university paper.
Vendors, please let me know by March 30th if you are interested.
If you miss this, and still would like mention around the conference, feel free to email me about sponsoring a break for food.
Students, more information will be forthcoming for you. With biological applications also mixing with material science these days, you may be interested if you mix the two disciplines as well.
Thanks!
Lou Ann Miller Biology Specialist, Frederick Seitz Materials Research Lab
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==============================Original Headers============================== 34, 29 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 28 07:23:18 2014 34, 29 -- Received: from znl.com ([206.69.208.20]) 34, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SDNIrK030295 34, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:18 -0600 34, 29 -- Received: from localhost (localhost [127.0.0.1]) 34, 29 -- by znl.com (Postfix) with ESMTP id A66DF6C78FB 34, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:18 -0600 (CST) 34, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 34, 29 -- Received: from znl.com ([127.0.0.1]) 34, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 34, 29 -- with ESMTP id Gf7Sp8RveAVg for {microscopy-at-microscopy.com} ; 34, 29 -- Fri, 28 Feb 2014 07:23:11 -0600 (CST) 34, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 34, 29 -- by znl.com (Postfix) with ESMTPA id 1294C6C78E9 34, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:11 -0600 (CST) 34, 29 -- Message-ID: {53108DBE.4060201-at-microscopy.com} 34, 29 -- Date: Fri, 28 Feb 2014 07:23:10 -0600 34, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 34, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 34, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 34, 29 -- MIME-Version: 1.0 34, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 34, 29 -- Subject: viaWWW:UIUC MRL Biological microscopy and analysis work shop Opportunity 34, 29 -- for vendors, and for students 34, 29 -- References: {201402271524.s1RFO1os024645-at-ns.microscopy.com} 34, 29 -- In-Reply-To: {201402271524.s1RFO1os024645-at-ns.microscopy.com} 34, 29 -- X-Forwarded-Message-Id: {201402271524.s1RFO1os024645-at-ns.microscopy.com} 34, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 34, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ghoshroy-at-sc.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
We have Denton DESK II Turbo Sputter Coater and are planning to use a chromium sputtering target for the first time. We intend to use this coating for FESEM observation of a variety of specimens.We are familiar with gold and gold-palladium targets but never used a chromium target before. This morning we coated a sample with chromium, but seems like it didn't get any coating at all. I did see the plasma. When we use gold or gold-palladium in the same coater, it works fine and we can see the coating on the surface. Is it possible to see a color difference after Cr coating for 60-100 sec. We are using 40-60% power.I noticed the Cr target is much thicker than the gold target.
I will appreciate someone telling if we are doing something wrong or it is just our simple ignorance about chromium target. Any help will be very much appreciated.
Thanks in advance,
Soumitra
Soumitra Ghoshroy EM Center University of South Carolina Columbia, SC 29208
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 28 07:23:51 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SDNpnZ030881 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:51 -0600 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id BDC366C790A 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:51 -0600 (CST) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id Ef0vpv91LP0l for {microscopy-at-microscopy.com} ; 16, 28 -- Fri, 28 Feb 2014 07:23:48 -0600 (CST) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id D2D156C78FD 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:23:48 -0600 (CST) 16, 28 -- Message-ID: {53108DE4.3060705-at-microscopy.com} 16, 28 -- Date: Fri, 28 Feb 2014 07:23:48 -0600 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW: Chromium sputtering target 16, 28 -- References: {201402271927.s1RJR9XD018573-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201402271927.s1RJR9XD018573-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201402271927.s1RJR9XD018573-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: gcat84-at-gmail.com Name: Glenn
Organization: University of Delaware
Title-Subject: [Filtered] ISI DS-130 - schematics
Message: Hello,
I have an ISI DS-130 in mostly working condition. However, it recently gave up on producing some of the +/- 15 V outputs. The first indication was when the vacuum would kick out when enabling the filament. Then, the emission displays 200 uA even when the voltage and current are set to zero.
Does anyone have original wiring diagrams for this model SEM unit? It would be greatly appreciated.
Regards, Glenn
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 28 07:24:48 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SDOkDM031961 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:24:46 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 057CB6C7919 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:24:46 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id aFg3cd7MvLfV for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 28 Feb 2014 07:24:44 -0600 (CST) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 9017E6C790C 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 07:24:44 -0600 (CST) 14, 28 -- Message-ID: {53108E1C.3080309-at-microscopy.com} 14, 28 -- Date: Fri, 28 Feb 2014 07:24:44 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Needed ISI DS-130 - schematics 14, 28 -- References: {201402280350.s1S3o1TQ017503-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201402280350.s1S3o1TQ017503-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201402280350.s1S3o1TQ017503-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I do not know the Denton Turbo Sputter Coater, but I was involved with the development of Chromium coating many years ago!
To sputter chromium is not like sputtering any of the more familiar materials. Firstly, you need a high vacuum and a power supply that is rated much higher than for the conventional sputtering materials. Secondly, before you are able to sputter the chromium the oxide coating has to be removed, and this has to happen every time you use the unit. So the first part of the sputter process is oxide removal, most dedicated systems have a shutter to catch the oxide, thus leave the specimens clean. You know when all of the oxide has been removed as the plasma will have a nice pale blue colour. Once you have this level of target cleaning you switch off the plasma, remove the protective shutter, and coat for about 2 to 5 seconds. You should not expect to be able to see the coating.
It's a very long time since I was involved with this work but I hope I have been able to provide some assistance?
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
We have Denton DESK II Turbo Sputter Coater and are planning to use a chromium sputtering target for the first time. We intend to use this coating for FESEM observation of a variety of specimens.We are familiar with gold and gold-palladium targets but never used a chromium target before. This morning we coated a sample with chromium, but seems like it didn't get any coating at all. I did see the plasma. When we use gold or gold-palladium in the same coater, it works fine and we can see the coating on the surface. Is it possible to see a color difference after Cr coating for 60-100 sec. We are using 40-60% power.I noticed the Cr target is much thicker than the gold target.
I will appreciate someone telling if we are doing something wrong or it is just our simple ignorance about chromium target. Any help will be very much appreciated.
Thanks in advance,
Soumitra
Soumitra Ghoshroy EM Center University of South Carolina Columbia, SC 29208
==============================Original Headers============================== 20, 24 -- From protrain-at-emcourses.com Fri Feb 28 08:03:44 2014 20, 24 -- Received: from mail.esentra.net (mail.esentra.net [94.228.36.208]) 20, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SE3h0X023155 20, 24 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 08:03:44 -0600 20, 24 -- Received: from mail.clientmail.net (Not Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway 20, 24 -- id {B5310973e0000} ; Fri, 28 Feb 2014 14:03:42 +0000 20, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Fri, 28 Feb 2014 14:03:41 +0000 20, 24 -- Reply-To: {protrain-at-emcourses.com} 20, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} 20, 24 -- To: {ghoshroy-at-sc.edu} 20, 24 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 20, 24 -- References: {201402281325.s1SDPkKA001008-at-ns.microscopy.com} 20, 24 -- In-Reply-To: {201402281325.s1SDPkKA001008-at-ns.microscopy.com} 20, 24 -- Subject: RE: [Microscopy] viaWWW: Chromium sputtering target 20, 24 -- Date: Fri, 28 Feb 2014 14:03:41 -0000 20, 24 -- Organization: Protrain 20, 24 -- Message-ID: {001d01cf348d$e3309a00$a991ce00$-at-com} 20, 24 -- MIME-Version: 1.0 20, 24 -- Content-Type: text/plain; 20, 24 -- charset="us-ascii" 20, 24 -- Content-Transfer-Encoding: 7bit 20, 24 -- X-Mailer: Microsoft Office Outlook 12.0 20, 24 -- Thread-Index: Ac80iJZe8ZT+E30bQTOloqRWS7R+/AAA2JOg 20, 24 -- Content-Language: en-gb ==============================End of - Headers==============================
We are working with an ESEM Quanta 600 in the low vacuum mode. We need a pressure of 0.18 Torr, but when we choose it via the UI the pressure is not constant but jumps between ~0.17 and ~0.28 Torr. At our second ESEM Quanta 200 we experience no problems with the pressure stability at 0.18 Torr.
Do you have an idea, which parameter or defect is responsible?
Thank you in advance!
Stefan
==============================Original Headers============================== 6, 30 -- From stefan.wernitznig-at-medunigraz.at Fri Feb 28 08:36:34 2014 6, 30 -- Received: from si068.medunigraz.at (si173.medunigraz.at [193.170.105.173]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SEaXUn009951 6, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 08:36:33 -0600 6, 30 -- X-AuditID: 0ac80c44-f79eb6d000000e72-07-53109ef1f9a8 6, 30 -- Received: from sj063.medunigraz.at (Unknown_Domain [10.200.18.79]) 6, 30 -- by si068.medunigraz.at (Symantec Mail Security) with SMTP id B2.87.03698.1FE90135; Fri, 28 Feb 2014 15:36:33 +0100 (CET) 6, 30 -- Received: from [10.200.112.128] (dc112128.medunigraz.at [10.200.112.128]) 6, 30 -- by sj063.medunigraz.at with ESMTP (TLS encrypted); Fri, 28 Feb 2014 15:36:27 +0100 6, 30 -- Message-ID: {53109EEF.8090506-at-medunigraz.at} 6, 30 -- Date: Fri, 28 Feb 2014 15:36:31 +0100 6, 30 -- From: Stefan Wernitznig {stefan.wernitznig-at-medunigraz.at} 6, 30 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:17.0) Gecko/20130801 Thunderbird/17.0.8 6, 30 -- MIME-Version: 1.0 6, 30 -- To: Microscopy-at-microscopy.com 6, 30 -- Subject: Pressure stability problems in the low vacuum mode of an ESEM 6, 30 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 6, 30 -- Content-Transfer-Encoding: 7bit 6, 30 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFlrDIsWRmVeSWpSXmKPExsXCdULIX/fjPIFgg6nTeCz+HG5gd2D0+Nj9 6, 30 -- mimAMYrLJiU1J7MstUjfLoEr487lB8wF85grdi9sYGtgPMzUxcjJISFgItG88xgLhC0mceHe 6, 30 -- erYuRi4OIYG9jBKr/h1lhnDmMUr8/TCTDaSKV0BHom3zTLAOFgFVifd321lBbDYBS4nj3T/A 6, 30 -- 4qICwRK7Hz9jhagXlDg58wlYXERAVmLB+yPsILawgJvEjb+fwK5gFrCR+HtiEQuELS+x/e0c 6, 30 -- 5gmMvLOQtM9CUjYLSdkCRuZVjMLFmQZmFnq5qSmleZnpRYlVeoklmxjBgcPjsoOxYYvhIUYB 6, 30 -- DkYlHl7pmQLBQqyJZcWVuYcYJTmYlER5788BCvEl5adUZiQWZ8QXleakFh9ilOBgVhLhfdIE 6, 30 -- lONNSaysSi3Kh0lJc7AoifN2hvMHCwmkJ5akZqemFqQWwWRlODiUJHifzAVqFCxKTU+tSMvM 6, 30 -- KUFIM3FwggznARpeArKYt7ggMbc4Mx0if4pRUkqcdx5IswBIIqM0D673FaM40AvCvO0gWR5g 6, 30 -- EoDregU0kAloIKcn2MCSRISUVAPjqZjlVVu4PGMfLLjTGnlrqQBb+sns1Brtts91338VWTre 6, 30 -- 1F2zam652PPeY/fXWEgmv9ubpnr15cz4+bmPlXpuPTv1cpf41zm+tSudry+bs/Vq0Nxo58um 6, 30 -- t3Rrcvczyp9qXCh46lzL2uXewokhVbsKGkRVOedfPzNvwu6blxKW7Vyxpn9G/X2xRCWW4oxE 6, 30 -- Qy3mouJEAFzE14i/AgAA ==============================End of - Headers==============================
Soumitra, as a compromise might I suggest using Pt, it will definitely give you better results than Au or Au/Pd.
Good Luck, John Robson
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Friday, February 28, 2014 9:13 AM To: Robson,John (AN) BIP-US-R
Hi
I do not know the Denton Turbo Sputter Coater, but I was involved with the development of Chromium coating many years ago!
To sputter chromium is not like sputtering any of the more familiar materials. Firstly, you need a high vacuum and a power supply that is rated much higher than for the conventional sputtering materials. Secondly, before you are able to sputter the chromium the oxide coating has to be removed, and this has to happen every time you use the unit. So the first part of the sputter process is oxide removal, most dedicated systems have a shutter to catch the oxide, thus leave the specimens clean. You know when all of the oxide has been removed as the plasma will have a nice pale blue colour. Once you have this level of target cleaning you switch off the plasma, remove the protective shutter, and coat for about 2 to 5 seconds. You should not expect to be able to see the coating.
It's a very long time since I was involved with this work but I hope I have been able to provide some assistance?
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
We have Denton DESK II Turbo Sputter Coater and are planning to use a chromium sputtering target for the first time. We intend to use this coating for FESEM observation of a variety of specimens.We are familiar with gold and gold-palladium targets but never used a chromium target before. This morning we coated a sample with chromium, but seems like it didn't get any coating at all. I did see the plasma. When we use gold or gold-palladium in the same coater, it works fine and we can see the coating on the surface. Is it possible to see a color difference after Cr coating for 60-100 sec. We are using 40-60% power.I noticed the Cr target is much thicker than the gold target.
I will appreciate someone telling if we are doing something wrong or it is just our simple ignorance about chromium target. Any help will be very much appreciated.
Thanks in advance,
Soumitra
Soumitra Ghoshroy EM Center University of South Carolina Columbia, SC 29208
==============================Original Headers============================== 20, 24 -- From protrain-at-emcourses.com Fri Feb 28 08:03:44 2014 20, 24 -- Received: from mail.esentra.net (mail.esentra.net [94.228.36.208]) 20, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SE3h0X023155 20, 24 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 08:03:44 -0600 20, 24 -- Received: from mail.clientmail.net (Not Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway 20, 24 -- id {B5310973e0000} ; Fri, 28 Feb 2014 14:03:42 +0000 20, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Fri, 28 Feb 2014 14:03:41 +0000 20, 24 -- Reply-To: {protrain-at-emcourses.com} 20, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} 20, 24 -- To: {ghoshroy-at-sc.edu} 20, 24 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 20, 24 -- References: {201402281325.s1SDPkKA001008-at-ns.microscopy.com} 20, 24 -- In-Reply-To: {201402281325.s1SDPkKA001008-at-ns.microscopy.com} 20, 24 -- Subject: RE: [Microscopy] viaWWW: Chromium sputtering target 20, 24 -- Date: Fri, 28 Feb 2014 14:03:41 -0000 20, 24 -- Organization: Protrain 20, 24 -- Message-ID: {001d01cf348d$e3309a00$a991ce00$-at-com} 20, 24 -- MIME-Version: 1.0 20, 24 -- Content-Type: text/plain; 20, 24 -- charset="us-ascii" 20, 24 -- Content-Transfer-Encoding: 7bit 20, 24 -- X-Mailer: Microsoft Office Outlook 12.0 20, 24 -- Thread-Index: Ac80iJZe8ZT+E30bQTOloqRWS7R+/AAA2JOg 20, 24 -- Content-Language: en-gb ==============================End of - Headers==============================
==============================Original Headers============================== 29, 26 -- From prvs=129e7d229=john.robson-at-boehringer-ingelheim.com Fri Feb 28 09:05:22 2014 29, 26 -- Received: from mail4.boehringer-ingelheim.com (mail4.boehringer-ingelheim.com [148.188.192.93]) 29, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SF5M5T029190 29, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 09:05:22 -0600 29, 26 -- Received: from NAHEXMB02.am.boehringer.com ([fe80::b19b:78f1:6b5f:e306]) by 29, 26 -- NAHEXCH02.am.boehringer.com ([fe80::d9b9:37f3:58a9:fb6%12]) with mapi id 29, 26 -- 14.02.0387.000; Fri, 28 Feb 2014 10:05:21 -0500 29, 26 -- From: {john.robson-at-boehringer-ingelheim.com} 29, 26 -- To: {Microscopy-at-microscopy.com} 29, 26 -- CC: {ghoshroy-at-sc.edu} 29, 26 -- Subject: RE: [Microscopy] RE: viaWWW: Chromium sputtering target 29, 26 -- Thread-Topic: [Microscopy] RE: viaWWW: Chromium sputtering target 29, 26 -- Thread-Index: AQHPNI83iyZcdqcy10meMJHXITB2dprKwrnA 29, 26 -- Date: Fri, 28 Feb 2014 15:05:21 +0000 29, 26 -- Message-ID: {0505FBCD63EE71428E13EBEEFC2C4E432275AC45-at-NAHEXMB02.am.boehringer.com} 29, 26 -- References: {201402281413.s1SEDBj3002903-at-ns.microscopy.com} 29, 26 -- In-Reply-To: {201402281413.s1SEDBj3002903-at-ns.microscopy.com} 29, 26 -- Accept-Language: en-US 29, 26 -- Content-Language: en-US 29, 26 -- X-MS-Has-Attach: 29, 26 -- X-MS-TNEF-Correlator: 29, 26 -- x-originating-ip: [10.128.230.253] 29, 26 -- Content-Type: text/plain; charset="us-ascii" 29, 26 -- MIME-Version: 1.0 29, 26 -- Content-Transfer-Encoding: 8bit 29, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1SF5M5T029190 ==============================End of - Headers==============================
Soumitra; Chromium is extremely reactive with oxygen. Even with a UHV system, chromium deposits tend to be chromium oxide. The best way to deposit chromium is with an ion beam sputtering system. Plasma sputtering, as you have discovered, does not work well for chromium. You see the glow of the plasma, but not much else happens.
John Mardinly, ASU
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, February 28, 2014 6:43 AM To: John Mardinly
X-from: ghoshroy-at-sc.edu ()
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We have Denton DESK II Turbo Sputter Coater and are planning to use a chromium sputtering target for the first time. We intend to use this coating for FESEM observation of a variety of specimens.We are familiar with gold and gold-palladium targets but never used a chromium target before. This morning we coated a sample with chromium, but seems like it didn't get any coating at all. I did see the plasma. When we use gold or gold-palladium in the same coater, it works fine and we can see the coating on the surface. Is it possible to see a color difference after Cr coating for 60-100 sec. We are using 40-60% power.I noticed the Cr target is much thicker than the gold target.
I will appreciate someone telling if we are doing something wrong or it is just our simple ignorance about chromium target. Any help will be very much appreciated.
Thanks in advance,
Soumitra
Soumitra Ghoshroy EM Center University of South Carolina Columbia, SC 29208
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==============================Original Headers============================== 24, 37 -- From John.Mardinly-at-asu.edu Fri Feb 28 13:36:04 2014 24, 37 -- Received: from bcnet7.asu.edu (bcnet7.asu.edu [129.219.117.222]) 24, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s1SJa4E3019852 24, 37 -- for {Microscopy-at-Microscopy.com} ; Fri, 28 Feb 2014 13:36:04 -0600 24, 37 -- X-ASG-Debug-ID: 1393616163-04dc7a6b0b3916c0001-FOsErg 24, 37 -- Received: from exhubt01.asurite.ad.asu.edu (exhubt01.asurite.ad.asu.edu [129.219.103.40]) by bcnet7.asu.edu with ESMTP id A1To56V6e2csQrh4 (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Fri, 28 Feb 2014 12:36:03 -0700 (MST) 24, 37 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 24, 37 -- X-Barracuda-Apparent-Source-IP: 129.219.103.40 24, 37 -- X-ASG-Whitelist: Client 24, 37 -- Received: from EXMBW02.asurite.ad.asu.edu ([169.254.9.176]) by 24, 37 -- exhubt01.asurite.ad.asu.edu ([129.219.103.40]) with mapi id 14.03.0174.001; 24, 37 -- Fri, 28 Feb 2014 12:36:03 -0700 24, 37 -- From: John Mardinly {John.Mardinly-at-asu.edu} 24, 37 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 24, 37 -- Subject: RE: [Microscopy] viaWWW: Chromium sputtering target 24, 37 -- Thread-Topic: [Microscopy] viaWWW: Chromium sputtering target 24, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] viaWWW: Chromium sputtering target 24, 37 -- Thread-Index: AQHPNIsH04ggBj0lsE2ks93J6PhfGprLDhfg 24, 37 -- Date: Fri, 28 Feb 2014 19:36:02 +0000 24, 37 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE940115E89F1-at-exmbw02.asurite.ad.asu.edu} 24, 37 -- References: {201402281343.s1SDhAdA005717-at-ns.microscopy.com} 24, 37 -- In-Reply-To: {201402281343.s1SDhAdA005717-at-ns.microscopy.com} 24, 37 -- Accept-Language: en-US 24, 37 -- Content-Language: en-US 24, 37 -- X-MS-Has-Attach: 24, 37 -- X-MS-TNEF-Correlator: 24, 37 -- x-originating-ip: [129.219.4.240] 24, 37 -- Content-Type: text/plain; charset="us-ascii" 24, 37 -- MIME-Version: 1.0 24, 37 -- X-Barracuda-Connect: exhubt01.asurite.ad.asu.edu[129.219.103.40] 24, 37 -- X-Barracuda-Start-Time: 1393616163 24, 37 -- X-Barracuda-Encrypted: AES128-SHA 24, 37 -- X-Barracuda-URL: http://129.219.117.222:8000/cgi-mod/mark.cgi 24, 37 -- X-Virus-Scanned: by bsmtpd at asu.edu 24, 37 -- X-Barracuda-BRTS-Status: 1 24, 37 -- Content-Transfer-Encoding: 8bit 24, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s1SJa4E3019852 ==============================End of - Headers==============================
On behalf of the organizing committee, I’d like to extend an invitation for you to attend the first Microscopy of Infectious Disease Agents Symposia we are hosting at Rocky Mountain Laboratories/NIAID/NIH in Hamilton, Montana July 24th and 25th. Please feel free to pass this along to anyone who might be interested in attending.
The 2014 Microscopy of Infectious Disease Agents Symposia (MIDAS) will provide an opportunity to bring together leading researchers of microscopic imaging and related techniques as they apply to the study of human pathogens. Organized by the National Institute of Allergy of Infectious Diseases, National Institutes of Health, this meeting will provide a venue for recent progress in areas of both light and electron microscopies relevant to imaging viruses, bacteria, prions, host cells and tissues. In addition to scientific presentations, workshops will be organized to teach various aspects of emerging technologies. The field of microscopic imaging is rapidly evolving as advances in both instrumentation and computational sciences are enabling a greatly expanding range of analyses to be performed. A broad theme at the conference will be on technological advances and on future developments needed to increase the application and impact of microscopic imaging for understanding the biology of human pathogens with the goal of improving the treatment, diagnosis, and prevention of infectious diseases. The conference will bring together a diverse group of scientists at senior and junior levels. Seminars and posters at the conference will provide an opportunity to present research findings and exchange of ideas.
Please visit our registration site for more information and the preliminary agenda. respond.niaid.nih.gov/conferences/rmlsymposium2014/Pages
Keynote speakers:
Sriram Subramaniam, Ph.D., National Cancer Institute, NIH 
Dr. Sriram Subramaniam received his Ph.D. in Physical Chemistry from Stanford University and completed postdoctoral training in the Departments of Chemistry and Biology at M.I.T. He is chief of the Biophysics Section in the Laboratory of Cell Biology at the Center for Cancer Research, National Cancer Institute. He holds a visiting faculty appointment at the Johns Hopkins University School of Medicine. His current work is focused on the development of advanced technologies for imaging macromolecular assemblies using 3D electron microscopy, and their application to address fundamental problems in HIV/AIDS and cancer research. Hari Shroff , Ph.D., National Ins titute of Biomedical Imaging and Bioengineering, NIH Dr. Hari Shroff received a B.S.E. in bioengineering from the University of Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed his Ph.D. in biophysics at the University of California at Berkeley in 2006. He spent the next three years performing postdoctoral research under the mentorship of Eric Betzig at the Howard Hughes Medical Institute's Janelia Farm Research Campus where his research focused on development of photo-activated localization microscopy (PALM), an optical super resolution technique. Dr. Shroff is now chief of NIBIB's section on high resolution optical imaging laboratory, where he and his staff are developing new imaging tools for application in biological and clinical research. Dr Shroff’s lab has recently developed two new microscope systems: The first, iSIM, uses structured illumination and high speed processing to render images with double the resolution of conventional fluorescence microscope. The second, diSPIM, is a light sheet microscope which uses two objectives to achieve isotropic resolution with minimal bleaching.
Organizing committee: Elizabeth Fischer, NIH/NIAID, Audray Harris, NIH/NIAID, and Owen Schwartz, NIH/NIAID
Contact: Elizabeth R. Fischer Microscopy Unit Manager Research Technologies Section/RTB Rocky Mountain Laboratories/NIAID/NIH 903 South 4th Street Hamilton, MT 59840 406-363-9378
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Email: tina.williams-at-ars.usda.gov Name: Tina Williams
Organization: USDA
Title-Subject: [Filtered] vacuum oven polymerization: Vent to hood?
Message: Hello,
While polymerizing your resins, do you vent your oven in a fume hood or to the lab? Safety suggestions? Thank you, Tina
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 28 18:19:10 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s210JAck028596 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 18:19:10 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 638116C7EEB 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 18:19:10 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id Rq334j911JgS for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 28 Feb 2014 18:19:08 -0600 (CST) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id BAFDF6C7EDE 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Feb 2014 18:19:08 -0600 (CST) 14, 28 -- Message-ID: {5311277C.5020807-at-microscopy.com} 14, 28 -- Date: Fri, 28 Feb 2014 18:19:08 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Question: vacuum oven polymerization: Vent to hood? 14, 28 -- References: {201402282342.s1SNggdX009382-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201402282342.s1SNggdX009382-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201402282342.s1SNggdX009382-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
PITTCON is not typically considered an important venue for microscopy, but there will be at least three dozen microscopy and related technology companies exhibiting at Chicago's McCormick Center, March 2-6. In addition, there will be a number of seminars, tutorials, and lunch&learn opportunities. American Lab asked that I do a review of Microscopy at PITTCON. That article is now posted at:
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realname - Pamela Coker, Ph.D. Email - pjcoker-at-pima.edu ORGANIZATION - Ph.D. EDUCATION - Undergraduate College LOCATION - Tucson, AZ 85743 SUBJECT_OF_QUESTION - Looking for a reference slide set for pharmaceuticals QUESTION - Hello,
I am writing a proposal for a new course in forensic microscopy. I have found most of the reference slide sets I need, however I am coming up empty handed on a pharmaceutical slide set.
Can anyone point me in the right direction? I'm not finding one at McCrone's. Thanks, Dr. C.
==============================Original Headers============================== 6, 27 -- From oshel1pe-at-cmich.edu Mon Mar 3 07:18:10 2014 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s23DIAXS002597 6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 07:18:10 -0600 6, 27 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s23DHuSw009468 6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 08:18:09 -0500 6, 27 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 6, 27 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 6, 27 -- 14.3.174.1; Mon, 3 Mar 2014 08:18:04 -0500 6, 27 -- Message-ID: {5314810C.3040809-at-cmich.edu} 6, 27 -- Date: Mon, 3 Mar 2014 08:18:04 -0500 6, 27 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 6, 27 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 27 -- MIME-Version: 1.0 6, 27 -- To: micro {Microscopy-at-microscopy.com} 6, 27 -- Subject: Ask-A-Microscopist: Reference slide set for pharmaceuticals needed 6, 27 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 27 -- Content-Transfer-Encoding: 7bit 6, 27 -- X-Originating-IP: [141.209.160.122] 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 27 -- X-Spam-Score: -0.50 () [Hold at 6.00] T_RP_MATCHES_RCVD,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 6, 27 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 27 -- X-CanItPRO-Stream: default 6, 27 -- X-Canit-Stats-ID: 02Lx1i9P6 - 0ff8d5c59c4d - 20140303 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I was wondering if there are digital versions of the user manuals for the Hitachi 7000 transmission EM and Hitachi S-3000N scanning EM? If so, I would very much appreciate a copy of them. Please contact me off-line and we can see how this can be arranged.
Many thanks, Debby
Debra Sherman, Founder & CSO DS imaging LLC Purdue Kurz Technology Center 1281 Win Hentschel Blvd West Lafayette, IN 47906 E-mail: debby.sherman-at-dsimagingllc.com www.dsimagingllc.com Mobile: 765-418-8540
==============================Original Headers============================== 8, 31 -- From dsherman-at-purdue.edu Mon Mar 3 11:37:59 2014 8, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s23Hbx7s024993 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 11:37:59 -0600 8, 31 -- Received: from WPVEXCHUB03.purdue.lcl (wpvexchub03.itap.purdue.edu [172.30.136.67]) 8, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s23Hbw4t008090 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 12:37:59 -0500 8, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([169.254.2.185]) by 8, 31 -- WPVEXCHUB03.purdue.lcl ([172.30.136.67]) with mapi id 14.03.0174.001; Mon, 3 8, 31 -- Mar 2014 12:37:58 -0500 8, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 8, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 31 -- Subject: Hitachi user manuals 8, 31 -- Thread-Topic: Hitachi user manuals 8, 31 -- Thread-Index: AQHPNwdRNT7eHhEgJ0W48koLW7AExg== 8, 31 -- Date: Mon, 3 Mar 2014 17:37:58 +0000 8, 31 -- Message-ID: {CF3A287D.1AC9F%dsherman-at-purdue.edu} 8, 31 -- Accept-Language: en-US 8, 31 -- Content-Language: en-US 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 8, 31 -- x-originating-ip: [24.14.170.252] 8, 31 -- Content-Type: text/plain; charset="us-ascii" 8, 31 -- Content-ID: {8C5FED44FF784340AB2715322C23B6F5-at-exchange.purdue.edu} 8, 31 -- MIME-Version: 1.0 8, 31 -- X-PMX-Version: 6.0.2.2308539 8, 31 -- X-PerlMx-URL-Scanned: Yes 8, 31 -- X-PerlMx-Virus-Scanned: Yes 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s23Hbx7s024993 ==============================End of - Headers==============================
Trying to address an electronics high temperature error on our Leo 906E TEM.
Would like to know where the temperature sensor is located on the system (column chassis or power supply rack) in order to troubleshoot the problem further.
Have thought for some time the issue was with the chiller but its running at 58 degrees F at a flow rate of 54 gph and the room is 70 degrees F.
On our coldest days this winter we have successfully run the instrument but we had a warm spell last week and the problem reappeared.
All rack cooling fans are running and filters are clean.
Any ideas are appreciated.
Roy Beavers Southern Methodist University rbeavers-at-smu.edu
==============================Original Headers============================== 10, 28 -- From rbeavers-at-mail.smu.edu Mon Mar 3 14:13:36 2014 10, 28 -- Received: from smtap2.systems.smu.edu (smtap2.systems.smu.edu [129.119.65.151]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s23KDavv007153 10, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 14:13:36 -0600 10, 28 -- X-IronPort-Anti-Spam-Filtered: true 10, 28 -- X-IronPort-Anti-Spam-Result: AvsMAI7hFFOBd0GH/2dsb2JhbABagmUhgRKzUDqMUYEmFnSCJwU6UQEqFEImAQQTCIdxAZ0UqUCGBReOCx2DXIEUBJRRlhaDLYIq 10, 28 -- X-IPAS-Result: AvsMAI7hFFOBd0GH/2dsb2JhbABagmUhgRKzUDqMUYEmFnSCJwU6UQEqFEImAQQTCIdxAZ0UqUCGBReOCx2DXIEUBJRRlhaDLYIq 10, 28 -- Received: from sxht1p4.systems.smu.edu ([129.119.65.135]) 10, 28 -- by smtah2.systems.smu.edu with ESMTP/TLS/AES128-SHA; 03 Mar 2014 14:13:36 -0600 10, 28 -- Received: from SXMB1PG.SYSTEMS.SMU.EDU ([fe80::1449:c186:5adb:ddbb]) by 10, 28 -- SXHT1P4.SYSTEMS.SMU.EDU ([fe80::4003:49d5:389b:a18%26]) with mapi id 10, 28 -- 14.02.0387.000; Mon, 3 Mar 2014 14:13:35 -0600 10, 28 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 10, 28 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 10, 28 -- Subject: Leo 906E TEM Temperature Issue 10, 28 -- Thread-Topic: Leo 906E TEM Temperature Issue 10, 28 -- Thread-Index: Ac83Gpswb/X1mDg2SCa+e+CZHSoYkw== 10, 28 -- Date: Mon, 3 Mar 2014 20:13:34 +0000 10, 28 -- Message-ID: {BC0C36E74D341741A25CE2ABB122E7A330B0B323-at-SXMB1PG.SYSTEMS.SMU.EDU} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- x-originating-ip: [129.119.65.139] 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s23KDavv007153 ==============================End of - Headers==============================
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Email: tina.williams-at-ars.usda.gov Name: Tina Williams
Organization: USDA
Title-Subject: [Filtered] vacuum oven polymerization: Vent to hood- Thanks for all the replies/suggestions
Message: Hello again,
Thank you to all who replied to my question regarding venting in a fume hood during polymerization of resins. I especially appreciated the references and safety reminders to reduce respiratory/skin/immuno sensitivities by venting in a fume hood.
I wanted to confirm, by having your collective experiences working with resins, that we continue working in harmony with these safety protocols. We have been venting the resin polymerization in the fume hood. However our current EHS officer, brought up the concern regarding the oven effecting the airflow in the hood. Since we have a large/heavy oven, and the same issue may apply with a small oven (if one could be found), we have kept the oven in the hood.
Your answers help us have a basis to continue with our current safety protocols, until a safer alternative is found.
Tina
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==============================Original Headers============================== 16, 29 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 3 18:49:21 2014 16, 29 -- Received: from znl.com ([206.69.208.20]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240nL3E009797 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:49:21 -0600 16, 29 -- Received: from localhost (localhost [127.0.0.1]) 16, 29 -- by znl.com (Postfix) with ESMTP id 1DB686CA2A4 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:49:21 -0600 (CST) 16, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 29 -- Received: from znl.com ([127.0.0.1]) 16, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 29 -- with ESMTP id Ir3pTYull+03 for {microscopy-at-microscopy.com} ; 16, 29 -- Mon, 3 Mar 2014 18:49:17 -0600 (CST) 16, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 29 -- by znl.com (Postfix) with ESMTPA id C67D36CA297 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:49:17 -0600 (CST) 16, 29 -- Message-ID: {5315230D.2080505-at-microscopy.com} 16, 29 -- Date: Mon, 03 Mar 2014 18:49:17 -0600 16, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 16, 29 -- MIME-Version: 1.0 16, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 29 -- Subject: viaWWW: vacuum oven polymerization: Vent to hood- Thanks for all 16, 29 -- the replies/suggestions 16, 29 -- References: {201403031952.s23JqWuL004732-at-ns.microscopy.com} 16, 29 -- In-Reply-To: {201403031952.s23JqWuL004732-at-ns.microscopy.com} 16, 29 -- X-Forwarded-Message-Id: {201403031952.s23JqWuL004732-at-ns.microscopy.com} 16, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: gr3g0rr33-at-gmail.com Name: Gregory Wyche
Organization: University of South Carolina
Title-Subject: [Filtered] Networking Opportunities In CT
Message: Hi, I'm a microscopist in CT (currently between jobs) looking for opportunities to network in that state. The Connecticut Microscopy Society website has not been updated in a long time so I can't get in touch with any of the members. If you know of any groups that meet, please forward them. I'll very much appreciate it.
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Email: dvanhart-at-binghamton.edu Name: Daniel VanHart
Organization: Binghamton University
Title-Subject: [Filtered] FTIR Microscope
Message: Does anyone have any experience with a Pike Technologies microMax Microscope?
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 3 18:52:36 2014 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240qYAR014362 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:52:34 -0600 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 49F996CA2C8 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:52:10 -0600 (CST) 11, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id R9rBs7d99M+l for {microscopy-at-microscopy.com} ; 11, 28 -- Mon, 3 Mar 2014 18:50:45 -0600 (CST) 11, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 024666CA2B5 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:45 -0600 (CST) 11, 28 -- Message-ID: {53152364.1080909-at-microscopy.com} 11, 28 -- Date: Mon, 03 Mar 2014 18:50:44 -0600 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:FTIR Microscope 11, 28 -- References: {201403032103.s23L3jd4026772-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201403032103.s23L3jd4026772-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201403032103.s23L3jd4026772-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: gul417-at-mail.usask.ca Name: Guosheng Liu
Organization: University of Saskatchewan
Title-Subject: [Filtered] TEM (Philips CM10) service contract provider?
Message: Hello EM Community,
We are shopping for a service contractor for our CM10 TEM, ideally from Canada or USA. This 20-year-old but sill reliable TEM has been service-contracted for most of its years but run without contract since early 2013(budge issue).....I was wondering if you would like to share information regarding service engineers/companies for the same or similar equipment. We will purchase a service coverage either very basic or all-inclusive depending on the quotes.
Thanks a lot.
Guosheng Liu
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 3 18:52:43 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240qhn5014397 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:52:43 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 84E4A6CA2D5 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:52:43 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id hL3J-Qia2Zkr for {microscopy-at-microscopy.com} ; 19, 28 -- Mon, 3 Mar 2014 18:51:58 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 886A46CA2C2 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:51:54 -0600 (CST) 19, 28 -- Message-ID: {531523AA.2060101-at-microscopy.com} 19, 28 -- Date: Mon, 03 Mar 2014 18:51:54 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:TEM (Philips CM10) service contract provider 19, 28 -- References: {201403032216.s23MGBJc027669-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201403032216.s23MGBJc027669-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201403032216.s23MGBJc027669-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You do know that the largest and most extensive microscopy meeting in the world will be held in Hartford CT, this August right?
http://www.microscopy.org/MandM/2014/index.cfm
There will certainly be opportunities for you to make connections there.
Nestor Your Friendly Neighborhood SysOp
On Mar 3, 2014, at 6:50 PM, microscopylistserver-noreply-at-microscopy.com wrote:
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=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 9700 S. Cass Ave. Argonne, Illinois 60439 USA Tel: 530-NES-TORZ (530-637-8679) Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow of the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past-President: Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University and University of Illinois at Chicago Visiting Professor of Materials Characterziation, Manchester University UK.
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==============================Original Headers============================== 22, 38 -- From anl.nestor.zaluzec-at-gmail.com Mon Mar 3 18:56:05 2014 22, 38 -- Received: from mail-ie0-f179.google.com (mail-ie0-f179.google.com [209.85.223.179]) 22, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240u5NJ023393 22, 38 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:56:05 -0600 22, 38 -- Received: by mail-ie0-f179.google.com with SMTP id lx4so1728441iec.24 22, 38 -- for {microscopy-at-microscopy.com} ; Mon, 03 Mar 2014 16:56:05 -0800 (PST) 22, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 22, 38 -- d=gmail.com; s=20120113; 22, 38 -- h=sender:subject:mime-version:content-type:from:in-reply-to:date:cc 22, 38 -- :content-transfer-encoding:message-id:references:to; 22, 38 -- bh=GdPVCeHPhQ+ONXCUSYiMrrAn5t2E3lT148JzpNSF3hI=; 22, 38 -- b=Cg8jZJgUObJ5QQQ/56ksAvc8Sa7peWNNuLmgjZszmuP3ecjZclOIVlt01i/nklAVRR 22, 38 -- JukVSIlwtIFsgxgJnmB/eXynzzCNvWBuIgQXhFyNLJTSPy7GQjZL3aiazPRmMMTVN86o 22, 38 -- 7BEkA9sxKh3+/7D1kaDgPdgRBAUICM9xsZ2Q8hoDkdbneGh+Oti3hVKbEv9JZxZqN1FE 22, 38 -- KDYAjRQgFdCiAIFquxA/5dVuZsvqhWb6OPTE7BYwVFQ7BkpZTINHqvILpYYxcPHp8SLi 22, 38 -- l854MIET/oXQNiXNCudLuR31gwawRYF1NbrR329/5usogJ0u/hq8ywedZ9TbcDBJgxf+ 22, 38 -- 4HMQ== 22, 38 -- X-Received: by 10.42.114.82 with SMTP id f18mr5636282icq.56.1393894565098; 22, 38 -- Mon, 03 Mar 2014 16:56:05 -0800 (PST) 22, 38 -- Received: from [192.168.1.101] (c-67-176-143-24.hsd1.il.comcast.net. [67.176.143.24]) 22, 38 -- by mx.google.com with ESMTPSA id gd5sm44564886igd.5.2014.03.03.16.56.03 22, 38 -- for {multiple recipients} 22, 38 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 22, 38 -- Mon, 03 Mar 2014 16:56:03 -0800 (PST) 22, 38 -- Sender: "ANL.Nestor Zaluzec" {anl.nestor.zaluzec-at-gmail.com} 22, 38 -- Subject: Re: [Microscopy] viaWWW:Networking Opportunities In CT 22, 38 -- Mime-Version: 1.0 (Apple Message framework v1283) 22, 38 -- Content-Type: text/plain; charset=us-ascii 22, 38 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 22, 38 -- In-Reply-To: {201403040050.s240o2Nf010560-at-ns.microscopy.com} 22, 38 -- Date: Mon, 3 Mar 2014 18:56:03 -0600 22, 38 -- Cc: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} , gr3g0rr33-at-gmail.com 22, 38 -- Message-Id: {9FE52B4F-3094-4693-A5C9-0D79E6A6DDA5-at-aaem.amc.anl.gov} 22, 38 -- References: {201403040050.s240o2Nf010560-at-ns.microscopy.com} 22, 38 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 38 -- X-Mailer: Apple Mail (2.1283) 22, 38 -- Content-Transfer-Encoding: 8bit 22, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s240u5NJ023393 ==============================End of - Headers==============================
Since you've used the web inquiry box to submit your question, I assume that you're not a MSA member. That's a big mistake. If you were a member, you could access a membership list that would give you contact information on 39 Connecticut members. Plus a very efficient job placement service. If you join now, you can also register for the Hartford meeting at the members' rate, a substantial saving.
Caroline
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==============================Original Headers============================== 6, 18 -- From schooley-at-mcn.org Mon Mar 3 19:59:38 2014 6, 18 -- Received: from smtp1.mcn.org (smtp1.mcn.org [216.150.240.85]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s241xcaZ004198 6, 18 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 19:59:38 -0600 6, 18 -- Received: from [66.81.52.3] 6, 18 -- by smtp1.mcn.org with esmtpa (Exim 4.82) 6, 18 -- (envelope-from {schooley-at-mcn.org} ) 6, 18 -- id 1WKeds-00079h-3L; Mon, 03 Mar 2014 17:59:37 -0800 6, 18 -- Mime-Version: 1.0 6, 18 -- Message-Id: {a06240803cf3ae0146dae-at-[66.81.52.3]} 6, 18 -- In-Reply-To: {201403040103.s2413x6s013563-at-ns.microscopy.com} 6, 18 -- References: {201403040103.s2413x6s013563-at-ns.microscopy.com} 6, 18 -- Date: Mon, 3 Mar 2014 17:58:33 -0800 6, 18 -- To: Gregory Wyche {gr3g0rr33-at-gmail.com} 6, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 6, 18 -- Subject: Re: [Microscopy] viaWWW:Networking Opportunities In CT 6, 18 -- Cc: microscopy-at-microscopy.com 6, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
If you use Google to search for Airy disk, and select the reference to the Wikipedia entry you will find a very extensive coverage of the subject of the Airy disk
While I'm at it, I can't resist including a story relating to this topic that I used to tell in my class when discussing TEM resolution;
A Cockney lady from London was touring New York City, and was taking a ride on the NY Subway. Now the subway trains push a rather strong blast of air ahead of them as they travel through the subway tunnels. When they approach a station this blast of air flows up through the opening creating quite a strong breeze. Well, our lady was alighting from one subway train just as another pulled into the station. The blast of air generated by this approaching train caught her skirts and blew them sky high, revealing the fact that she was not wearing any underwear. She, of course, acted terribly embarrassed, and a conductor standing nearby and seeing her distress thought he should say something to smooth over the situation. So, he said, "Airy, isn't it?" to which the lady replied in her native Cockney accent,"Wot in 'ell did ye expect, feathers?" -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 32 -- From bigelow-at-umich.edu Mon Mar 3 21:23:37 2014 3, 32 -- Received: from mail-ie0-f169.google.com (mail-ie0-f169.google.com [209.85.223.169]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s243Nb7Y029463 3, 32 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 21:23:37 -0600 3, 32 -- Received: by mail-ie0-f169.google.com with SMTP id to1so870485ieb.14 3, 32 -- for {microscopy-at-microscopy.com} ; Mon, 03 Mar 2014 19:23:37 -0800 (PST) 3, 32 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 32 -- d=1e100.net; s=20130820; 3, 32 -- h=x-gm-message-state:mime-version:message-id:date:to:from:subject 3, 32 -- :content-type; 3, 32 -- bh=cR1Ar2hgZaWrXtPzyT6QDs1jQ7xa2hFmZD2f2XBysFs=; 3, 32 -- b=aa6u4uGkqPdNG6FyNcpnaVxlytbbxODnFLbqzW0Cr/MdxXaub1pwawrzImQAfd0W1d 3, 32 -- +Vc1xJss/Z28xfF7kmX9/BdE4FkqIKtMacwjiZarRETl9BAgjIzzKhhOjE3D42h+r+sK 3, 32 -- 3Dn1OnM9Rn9e/Xl12Egt8uExQ7i1NeXdBzxdL8uiCpfESCmOXcbJ80Nt9/DEAaNRnoUf 3, 32 -- pu26Uyowll7oK4nAvJnozdjy3M0BZTyDX6rIKz/6CkTSV/s0DDh344ZkyuaCjBfz3dcN 3, 32 -- qU0VgsOQCQp/WV8Zj5O33XKNtHDWWE/4w73u0Uocbg3FLpqzZO6NjtzHRRf6Xq/nl6WC 3, 32 -- Jm4A== 3, 32 -- X-Gm-Message-State: ALoCoQnNbUfCm5A9TZ8sQsbJGgMLwH1ts0RtdIAKbBSGthNSzYC0oSOkg0L3ZAyy1sRcZwZxTyS7 3, 32 -- X-Received: by 10.50.153.115 with SMTP id vf19mr26627867igb.9.1393903417025; 3, 32 -- Mon, 03 Mar 2014 19:23:37 -0800 (PST) 3, 32 -- Received: from [76.226.27.46] ([76.226.27.46]) 3, 32 -- by mx.google.com with ESMTPSA id vu3sm45692983igc.6.2014.03.03.19.23.35 3, 32 -- for {microscopy-at-microscopy.com} 3, 32 -- (version=TLSv1 cipher=RC4-SHA bits=128/128); 3, 32 -- Mon, 03 Mar 2014 19:23:36 -0800 (PST) 3, 32 -- Mime-Version: 1.0 3, 32 -- Message-Id: {p06240800cf3af3544138-at-[76.226.27.46]} 3, 32 -- Date: Mon, 3 Mar 2014 22:22:11 -0500 3, 32 -- To: Microscopy {microscopy-at-microscopy.com} 3, 32 -- From: Wil Bigelow {bigelow-at-umich.edu} 3, 32 -- Subject: [Microscopy]RE#' Airy Disk 3, 32 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
the 4th UniBe Practical Course on Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS, a.k.a cryoEM of frozen-hydrated sections)
11-13 August 2014 / 14-16 August 2014
Organiser : Benoît Zuber Instructors: Benoît Zuber, Daniel Studer, Ioan Iacovache
The objective of the course is to teach the participants the practical skills necessary to successfully apply CEMOVIS in their laboratories. Essential background theory of CEMOVIS will be given and most of the time will be spent practicing high-pressure freezing, cryo-sectioning, and low-dose TEM imaging. 1 high-pressure freezing machine, 3 state-of-the-art cryo-ultramicrotomes and 1 cryo-electron microscope will be dedicated to the course. Of special interest, participants will learn to use the new tools that we developed to facilitate cryosectioning (Studer et al 2014 J Struct Biol 185(1):125-8). The 3-day course is given twice, each time with a maximum of 4 participants so that they can be actively practicing at any time.
The course is intended for scientists whose research projects will benefit from the use of CEMOVIS. Experience in either cryo-electron microscopy or ultramicrotomy of resin-embedded specimens is a prerequisite.
Information and registration:
http://www.ana.unibe.ch/events/cemovis/index.html
Registration deadline: 15.04.2014
Yours faithfully
Benoît Zuber
Prof. Benoît Zuber Institute of Anatomy University of Bern Baltzerstrasse 2 CH-3000 Bern 9 Switzerland Tel. +41 31 631 84 40 benoit.zuber-at-ana.unibe.ch http://www.ana.unibe.ch/~exmo/
==============================Original Headers============================== 16, 29 -- From benoit.zuber.work-at-gmail.com Tue Mar 4 03:36:18 2014 16, 29 -- Received: from mail-ob0-f193.google.com (mail-ob0-f193.google.com [209.85.214.193]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s249aIWs020921 16, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 03:36:18 -0600 16, 29 -- Received: by mail-ob0-f193.google.com with SMTP id gq1so2825936obb.8 16, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 04 Mar 2014 01:36:18 -0800 (PST) 16, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 29 -- d=gmail.com; s=20120113; 16, 29 -- h=mime-version:date:message-id:subject:from:to:content-type 16, 29 -- :content-transfer-encoding; 16, 29 -- bh=lKWk/ACoLkqIi4/lq3jocmTMNTEKRyWsIpH4aGUgEDc=; 16, 29 -- b=VlLJd0vQh98Qse/9mJ0pJ0f2OYivEGu38uGznV2kAQUYsOqJLggfhOoV7fxC4Nt/q6 16, 29 -- jkGC+nhqSFnUGomdGBUZ4khFHA8DCpmykCmVN0/UNkOfSslDGrBJV6EqgFAzesj8QYN6 16, 29 -- zgM1rq1/L++8DUr1FQXlmcCxCMGD7LC14qEJTWDWJPBuegDjRSJtO1AijblLo+vQu0/1 16, 29 -- Q7ZgbJQm77V017HhSrr3ysEoip/wvp0G/PbH6hhgHPakqMxd7eHPAY+vXGlIlPAhTv2Z 16, 29 -- 0Yx1kTDUFbsCLsvUnc82eBZe8c0tWlNdu8Yj0khuUX15BfjQR4dHi6R4bdU2nYhMwAHX 16, 29 -- QUzg== 16, 29 -- MIME-Version: 1.0 16, 29 -- X-Received: by 10.182.102.134 with SMTP id fo6mr31490668obb.10.1393925778214; 16, 29 -- Tue, 04 Mar 2014 01:36:18 -0800 (PST) 16, 29 -- Received: by 10.76.2.73 with HTTP; Tue, 4 Mar 2014 01:36:18 -0800 (PST) 16, 29 -- Date: Tue, 4 Mar 2014 10:36:18 +0100 16, 29 -- Message-ID: {CAF5rG8TmyMNc=wpQKVR+wnG5o+Cw7LP732R+e08K4jw6V4cOLA-at-mail.gmail.com} 16, 29 -- Subject: CEMOVIS / frozen-hydrated sections course announcement 16, 29 -- From: =?ISO-8859-1?Q?Beno=EEt_Zuber?= {benoit.zuber.work-at-gmail.com} 16, 29 -- To: Microscopy-at-microscopy.com 16, 29 -- Content-Type: text/plain; charset=ISO-8859-1 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s249aIWs020921 ==============================End of - Headers==============================
You might want to consider the New England Society for Microscopy (NESM - http://www.nesmicroscopy.org/). They area a good bunch of people who hold several meetings a year, typically in the eastern MA area.
Jeff
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Monday, March 03, 2014 8:11 PM To: Jeff Hall
X-from: gr3g0rr33-at-gmail.com ()
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Email: gr3g0rr33-at-gmail.com Name: Gregory Wyche
Organization: University of South Carolina
Title-Subject: [Filtered] Networking Opportunities In CT
Message: Hi, I'm a microscopist in CT (currently between jobs) looking for opportunities to network in that state. The Connecticut Microscopy Society website has not been updated in a long time so I can't get in touch with any of the members. If you know of any groups that meet, please forward them. I'll very much appreciate it.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 3 18:50:02 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240o2kB010547 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:02 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id 768ED6CA2B3 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:02 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id TlwPJ2eaIOP0 for {microscopy-at-microscopy.com} ; 12, 28 -- Mon, 3 Mar 2014 18:50:01 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id 36DBC6CA2A6 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:01 -0600 (CST) 12, 28 -- Message-ID: {53152339.7040806-at-microscopy.com} 12, 28 -- Date: Mon, 03 Mar 2014 18:50:01 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Networking Opportunities In CT 12, 28 -- References: {201403032055.s23Ktm2d026609-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201403032055.s23Ktm2d026609-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201403032055.s23Ktm2d026609-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 21, 22 -- From JHall-at-2SPI.COM Tue Mar 4 08:25:03 2014 21, 22 -- Received: from mail.2spi.com (mail.2spi.com [209.120.197.107]) 21, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s24EP2S6012228 21, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 08:25:02 -0600 21, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 21, 22 -- Content-class: urn:content-classes:message 21, 22 -- MIME-Version: 1.0 21, 22 -- Content-Type: text/plain; 21, 22 -- charset="us-ascii" 21, 22 -- Subject: RE: [Microscopy] viaWWW:Networking Opportunities In CT 21, 22 -- Date: Tue, 4 Mar 2014 09:24:59 -0500 21, 22 -- Message-ID: {BED026C9A599074CA181C34E28E3A182013FCCF0-at-SBSERVER1.2spi.local} 21, 22 -- In-Reply-To: {201403040110.s241AbYg002661-at-ns.microscopy.com} 21, 22 -- X-MS-Has-Attach: 21, 22 -- X-MS-TNEF-Correlator: 21, 22 -- Thread-Topic: [Microscopy] viaWWW:Networking Opportunities In CT 21, 22 -- Thread-Index: Ac83Ro7dPO5MpLxdTmKwel2Wr9GkngAbqx2Q 21, 22 -- References: {201403040110.s241AbYg002661-at-ns.microscopy.com} 21, 22 -- From: "Jeff Hall" {JHall-at-2SPI.COM} 21, 22 -- To: {Microscopy-at-microscopy.com} 21, 22 -- Content-Transfer-Encoding: 8bit 21, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s24EP2S6012228 ==============================End of - Headers==============================
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Monday, March 03, 2014 7:57 PM To: kenconverse-at-qualityimages.biz
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Email: gul417-at-mail.usask.ca Name: Guosheng Liu
Organization: University of Saskatchewan
Title-Subject: [Filtered] TEM (Philips CM10) service contract provider?
Message: Hello EM Community,
We are shopping for a service contractor for our CM10 TEM, ideally from Canada or USA. This 20-year-old but sill reliable TEM has been service-contracted for most of its years but run without contract since early 2013(budge issue).....I was wondering if you would like to share information regarding service engineers/companies for the same or similar equipment. We will purchase a service coverage either very basic or all-inclusive depending on the quotes.
Thanks a lot.
Guosheng Liu
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 3 18:52:43 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240qhn5014397 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:52:43 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 84E4A6CA2D5 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:52:43 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id hL3J-Qia2Zkr for {microscopy-at-microscopy.com} ; 19, 28 -- Mon, 3 Mar 2014 18:51:58 -0600 (CST) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 886A46CA2C2 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:51:54 -0600 (CST) 19, 28 -- Message-ID: {531523AA.2060101-at-microscopy.com} 19, 28 -- Date: Mon, 03 Mar 2014 18:51:54 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:TEM (Philips CM10) service contract provider 19, 28 -- References: {201403032216.s23MGBJc027669-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201403032216.s23MGBJc027669-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201403032216.s23MGBJc027669-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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==============================Original Headers============================== 34, 27 -- From kenconverse-at-qualityimages.biz Tue Mar 4 08:28:38 2014 34, 27 -- Received: from cdptpa-oedge-vip.email.rr.com (cdptpa-outbound-snat.email.rr.com [107.14.166.231]) 34, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s24ESb3H014075 34, 27 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 08:28:38 -0600 34, 27 -- Received: from [108.183.173.180] ([108.183.173.180:2080] helo=Ken) 34, 27 -- by cdptpa-oedge03 (envelope-from {kenconverse-at-qualityimages.biz} ) 34, 27 -- (ecelerity 3.5.0.35861 r(Momo-dev:tip)) with ESMTP 34, 27 -- id E6/F9-26685-513E5135; Tue, 04 Mar 2014 14:28:37 +0000 34, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 34, 27 -- To: {gul417-at-mail.usask.ca} , "MSA Listserver" {microscopy-at-microscopy.com} 34, 27 -- Subject: RE: [Microscopy] viaWWW:TEM (Philips CM10) service contract provider 34, 27 -- Date: Tue, 4 Mar 2014 09:30:34 -0500 34, 27 -- Message-ID: {EBB472050E0D4074AAAF413F43532B1B-at-Ken} 34, 27 -- MIME-Version: 1.0 34, 27 -- Content-Type: text/plain; 34, 27 -- charset="us-ascii" 34, 27 -- X-Priority: 3 (Normal) 34, 27 -- X-MSMail-Priority: Normal 34, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 34, 27 -- In-Reply-To: {201403040057.s240vIaN027941-at-ns.microscopy.com} 34, 27 -- Importance: Normal 34, 27 -- Thread-Index: Ac83RLWJE60Tz5FCQfGsb4avGWcejgAcR7Ug 34, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 34, 27 -- X-RR-Connecting-IP: 107.14.168.142:25 34, 27 -- X-Cloudmark-Score: 0 34, 27 -- Content-Transfer-Encoding: 8bit 34, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s24ESb3H014075 ==============================End of - Headers==============================
Gregory, Try the New England Society for Microscopy. They are very active. www.nesmicroscopy.org
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Monday, March 03, 2014 7:54 PM To: kenconverse-at-qualityimages.biz
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Email: gr3g0rr33-at-gmail.com Name: Gregory Wyche
Organization: University of South Carolina
Title-Subject: [Filtered] Networking Opportunities In CT
Message: Hi, I'm a microscopist in CT (currently between jobs) looking for opportunities to network in that state. The Connecticut Microscopy Society website has not been updated in a long time so I can't get in touch with any of the members. If you know of any groups that meet, please forward them. I'll very much appreciate it.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 3 18:50:02 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s240o2kB010547 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:02 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id 768ED6CA2B3 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:02 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id TlwPJ2eaIOP0 for {microscopy-at-microscopy.com} ; 12, 28 -- Mon, 3 Mar 2014 18:50:01 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id 36DBC6CA2A6 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2014 18:50:01 -0600 (CST) 12, 28 -- Message-ID: {53152339.7040806-at-microscopy.com} 12, 28 -- Date: Mon, 03 Mar 2014 18:50:01 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Networking Opportunities In CT 12, 28 -- References: {201403032055.s23Ktm2d026609-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201403032055.s23Ktm2d026609-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201403032055.s23Ktm2d026609-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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==============================Original Headers============================== 25, 27 -- From kenconverse-at-qualityimages.biz Tue Mar 4 08:33:53 2014 25, 27 -- Received: from cdptpa-oedge-vip.email.rr.com (cdptpa-outbound-snat.email.rr.com [107.14.166.228]) 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s24EXrCZ021904 25, 27 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 08:33:53 -0600 25, 27 -- Received: from [108.183.173.180] ([108.183.173.180:2097] helo=Ken) 25, 27 -- by cdptpa-oedge03 (envelope-from {kenconverse-at-qualityimages.biz} ) 25, 27 -- (ecelerity 3.5.0.35861 r(Momo-dev:tip)) with ESMTP 25, 27 -- id C6/0F-26685-054E5135; Tue, 04 Mar 2014 14:33:52 +0000 25, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 25, 27 -- To: {gr3g0rr33-at-gmail.com} , "MSA Listserver" {microscopy-at-microscopy.com} 25, 27 -- Subject: RE: [Microscopy] viaWWW:Networking Opportunities In CT 25, 27 -- Date: Tue, 4 Mar 2014 09:35:51 -0500 25, 27 -- Message-ID: {1E7A1A53EEC74BC49338D1379214F732-at-Ken} 25, 27 -- MIME-Version: 1.0 25, 27 -- Content-Type: text/plain; 25, 27 -- charset="us-ascii" 25, 27 -- X-Priority: 3 (Normal) 25, 27 -- X-MSMail-Priority: Normal 25, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 25, 27 -- In-Reply-To: {201403040054.s240sP02017968-at-ns.microscopy.com} 25, 27 -- Importance: Normal 25, 27 -- Thread-Index: Ac83RFFmOk/rE4OlS/SuvQ0oXVz9rQAcl+kg 25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 25, 27 -- X-RR-Connecting-IP: 107.14.168.142:25 25, 27 -- X-Cloudmark-Score: 0 25, 27 -- Content-Transfer-Encoding: 8bit 25, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s24EXrCZ021904 ==============================End of - Headers==============================
Dear Listers, if there's a hole in the window of an EDS detector mounted on a TEM, what problem may arise using the detector? I realizd that it's clear to me the window purpose in a SEM, where the chamber is frequently vented, but what about in a TEM? Thanks
The purpose is to keep the detector crystal clean. Without the window there, the detector will behave very much like a cold finger in any other high or ultra high vacuum system. Residual water and other volatiles in the system will condense on the crystal surface over time. The effect may not be as immediate as it would be at atmospheric pressure, but it can be just as devastating.
Windowless detectors can be used if the vacuum quality is very high (UHV conditions with very little hydrocarbon contamination) or the detector is relatively warm as with an SDD. Even with a windowless SDD, care must be taken to ensure that the vacuum quality remains relatively high, though UHV conditions are not required.
-Jacob
On 14-03-04 08:44 AM, dcristofori-at-unive.it wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } if there's a hole in the window of an EDS detector mounted on a TEM, } what problem may arise using the detector? } I realizd that it's clear to me the window purpose in a SEM, where the } chamber is frequently vented, but what about in a TEM? } Thanks } } Davide } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive[dot]it } } Universitŕ Ca' Foscari Venezia } Dipartimento di Sc. Molecolari e Nanosistemi } Via Torino, 155b I-30172 Mestre (VE) Italy } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } } ==============================Original Headers============================== } 6, 20 -- From dcristofori-at-unive.it Tue Mar 4 10:33:15 2014 } 6, 20 -- Received: from orion.unive.it (orion.unive.it [157.138.1.25]) } 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s24GXFdq008244 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 10:33:15 -0600 } 6, 20 -- Received: by orion.unive.it (Postfix, from userid 498) } 6, 20 -- id 024D34275D; Tue, 4 Mar 2014 17:33:11 +0100 (CET) } 6, 20 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) } 6, 20 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) } 6, 20 -- (No client certificate requested) } 6, 20 -- by orion.unive.it (Postfix) with ESMTPSA id D661B42733 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 17:33:11 +0100 (CET) } 6, 20 -- Message-ID: {5316076C.6090101-at-unive.it} } 6, 20 -- Date: Tue, 04 Mar 2014 18:03:40 +0100 } 6, 20 -- From: Davide Cristofori {dcristofori-at-unive.it} } 6, 20 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 } 6, 20 -- MIME-Version: 1.0 } 6, 20 -- To: Microscopy-at-microscopy.com } 6, 20 -- Subject: TEM - EDS window purpose and holes } 6, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 6, 20 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } }
-- Jacob Kabel Electron Microscopist Department of Materials Engineering The University of British Columbia 6350 Stores Road, Room 419 Vancouver, British Columbia Canada V6T 1Z4 Phone: (604) 822-5648 Fax: (604) 822-3619 http://emlab.mtrl.ubc.ca/
==============================Original Headers============================== 7, 28 -- From jkabel-at-mail.ubc.ca Tue Mar 4 10:56:55 2014 7, 28 -- Received: from vmaprod2.mail-relay.ubc.ca (vmaprod2.mail-relay.ubc.ca [142.103.117.133]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s24GutmA029329 7, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 10:56:55 -0600 7, 28 -- Received: from vmaprod2.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 7, 28 -- by localhost (Email Security Appliance) with SMTP id C01E716738A_31605D6B 7, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 16:56:54 +0000 (GMT) 7, 28 -- Received: from mx3.mail-relay.ubc.ca (lb_mx-out.mail-relay.ubc.ca [10.92.8.24]) 7, 28 -- by vmaprod2.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTP id 3FD4916730E_31605D6F 7, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 16:56:54 +0000 (GMT) 7, 28 -- Received: from smtp.mail.ubc.ca (s-itsv-hub02p.ead.ubc.ca [137.82.151.71]) 7, 28 -- by mx3.mail-relay.ubc.ca (Postfix) with ESMTP id 3A22D15F92B 7, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 08:56:54 -0800 (PST) 7, 28 -- Received: from [137.82.16.57] (137.82.16.57) by smtp.mail.ubc.ca 7, 28 -- (137.82.151.71) with Microsoft SMTP Server id 14.3.174.1; Tue, 4 Mar 2014 7, 28 -- 08:56:54 -0800 7, 28 -- Message-ID: {531605D9.80504-at-mail.ubc.ca} 7, 28 -- Date: Tue, 4 Mar 2014 08:56:57 -0800 7, 28 -- From: Jacob Kabel {jkabel-at-mail.ubc.ca} 7, 28 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 7, 28 -- MIME-Version: 1.0 7, 28 -- To: {Microscopy-at-microscopy.com} 7, 28 -- Subject: Re: [Microscopy] TEM - EDS window purpose and holes 7, 28 -- References: {201403041644.s24GiFq9024885-at-ns.microscopy.com} 7, 28 -- In-Reply-To: {201403041644.s24GiFq9024885-at-ns.microscopy.com} 7, 28 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-Originating-IP: [137.82.16.57] ==============================End of - Headers==============================
The program for the upcoming 3DEM GRC is mostly online now: http://www.grc.org/programs.aspx?year=2014&program=threedim This years's focus will be on "Technical Advances for a Rising Star in Structural Biology: 3DEM". The site in Spain can house 200 guests. Over 100 participants are already registered, the conference is filling up quickly. The registration deadline is May 25. However, earlier registrations may be accepted sooner, in order to allow you to make travel arrangements in time.
Looking forward to meeting you in Spain for an exciting conference,
Henning.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62 | mailto:Henning.Stahlberg-at-unibas.ch
==============================Original Headers============================== 10, 36 -- From henning.stahlberg-at-unibas.ch Tue Mar 4 11:59:20 2014 10, 36 -- Received: from mx1-priv.urz.unibas.ch (mx1-priv.urz.unibas.ch [131.152.226.164]) 10, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s24HxK2Y022780 10, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 11:59:20 -0600 10, 36 -- Received: from localhost (localhost [127.0.0.1]) 10, 36 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTP id 5D57E3E0735; 10, 36 -- Tue, 4 Mar 2014 18:59:19 +0100 (CET) 10, 36 -- X-Virus-Scanned: amavisd-new at unibas.ch 10, 36 -- Received: from mx1-priv.urz.unibas.ch ([131.152.226.164]) 10, 36 -- by localhost (mx1-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 10, 36 -- with LMTP id xMEltNcO66z9; Tue, 4 Mar 2014 18:59:19 +0100 (CET) 10, 36 -- Received: from URZ-HT-CAS-2.urz.unibas.ch (urz-ht-cas-2.urz.unibas.ch [131.152.8.132]) 10, 36 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTPS id 451BE3E0734; 10, 36 -- Tue, 4 Mar 2014 18:59:19 +0100 (CET) 10, 36 -- Received: from URZ-MBX-3.urz.unibas.ch ([131.152.8.143]) by 10, 36 -- URZ-HT-CAS-2.urz.unibas.ch ([fe80::69cb:c9f0:b340:3e32%12]) with mapi id 10, 36 -- 14.03.0174.001; Tue, 4 Mar 2014 18:59:19 +0100 10, 36 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 10, 36 -- To: 3dem 3dem {3dem-at-ucsd.edu} , 10, 36 -- "Microscopy-at-microscopy.com" 10, 36 -- {Microscopy-at-microscopy.com} 10, 36 -- Subject: 3DEM GRC in Girona, Spain, June 22-27, 2014 10, 36 -- Thread-Topic: 3DEM GRC in Girona, Spain, June 22-27, 2014 10, 36 -- Thread-Index: AQHPN9N2M4RFKGJs0kOI58uZsqygHg== 10, 36 -- Date: Tue, 4 Mar 2014 17:59:18 +0000 10, 36 -- Message-ID: {7BB87917-F808-4479-95D1-FD6F040C9532-at-unibas.ch} 10, 36 -- Accept-Language: en-US, de-CH 10, 36 -- Content-Language: en-US 10, 36 -- X-MS-Has-Attach: 10, 36 -- X-MS-TNEF-Correlator: 10, 36 -- x-originating-ip: [129.132.128.85] 10, 36 -- Content-Type: text/plain; charset="us-ascii" 10, 36 -- Content-ID: {2DD2D83C4943A0498A1A6E44685FB3CF-at-zuv.ads.unibas.ch} 10, 36 -- MIME-Version: 1.0 10, 36 -- Content-Transfer-Encoding: 8bit 10, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s24HxK2Y022780 ==============================End of - Headers==============================
EBSD 2014 is a topical conference sponsored by the Microanalysis Society (MAS) and will be held this June 17-19 at Carnegie Mellon University, Pittsburgh, PA, USA. This conference is a three-day event, highlighted by a one-day tutorial on the electron backscatter diffraction (EBSD) technique and two-days of technical symposia featuring invited and contributed talks on EBSD development and applications in materials science and geological studies.
Registration is currently open, but space is limited and registration slots are filling up quickly (deadline is March 31). Please see the link below for more information about the conference, to register, and to submit abstracts for oral/poster presentations (Abstract deadline is April 2): http://www.microbeamanalysis.org/topical-conferences/EBSD-2014/welcome
Below is more details about the tutorial and technical symposia for EBSD 2014. We sincerely hope you can attend!
Tutorials - Tuesday, June 17th Topics and speakers are listed below: EBSD Technology (Des Moser and Andy Deal) Sample Preparation (Brian Hess and Ron Witt) Data Acquisition and Post Processing (David Mainprice) Free, Open-source MTEX Data Processing Tools (David Mainprice) Case Studies (David Mainprice and Joe Michael)
Live Demonstrations - Tuesday, June 17th In parallel with the tutorial sessions, there will be opportunities to test out the latest and greatest EBSD systems from all major EBSD vendors (including Bruker, EDAX, Oxford Instruments, Thermo-Fisher).
Technical Symposia - Wednesday & Thursday, June 18th &19th The EBSD 2014 technical symposia are the heart of the conference and consist of an exciting group of invited speakers at the forefront of EBSD technology and its applications. Below are the session topics and invited speakers headlining each session:
EBSD for Industry - Raj Mishra - General Motors, USA EBSD and Energy Technologies - Helio Moutinho - National Renewable Energy Laboratory, USA Microstructure Characterization - Stefan Zaefferer - Max Planck Institute, Germany Computational Advances - Gert Nolze - BAM, Germany Novel Methods - Pat Trimby - University of Sydney, Australia Geochronology Phases - Steve Reddy - Curtin University, Australia Deformation Fabrics - David Prior - University of Otago, New Zealand
Thank you for your interest and looking forward to seeing you at the conference this June!
EBSD 2014 Organizing Committee David Fullwood - Brigham Young University Elena Miranda - California State University - Northridge Desmond Moser - The University of Western Ontario Yoosuf Picard - Carnegie Mellon University Andrew Deal - GE Global Research
John Mansfield PhD Cphys MInstP *****Note New Business Mailing Address, all other information remains the same***** Microscopy Society of America - President-Elect Associate Director North Campus Electron Microbeam Analysis Laboratory Building 22, Room G010, University of Michigan 2800 Plymouth Road, Ann Arbor MI 48109-2800 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/ iMessage: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096
==============================Original Headers============================== 6, 17 -- From jfmjfm-at-umich.edu Tue Mar 4 12:49:18 2014 6, 17 -- Received: from emalserver.engin.umich.edu (emalserver.engin.umich.edu [141.213.21.62]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s24InI94014552 6, 17 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 12:49:18 -0600 6, 17 -- Received: from [172.17.200.5] (unknown [172.17.200.5]) 6, 17 -- by emalserver.engin.umich.edu (Postfix) with ESMTP id 6EC741646CAE; 6, 17 -- Tue, 4 Mar 2014 13:49:18 -0500 (EST) 6, 17 -- From: John Mansfield {jfmjfm-at-umich.edu} 6, 17 -- Content-Type: text/plain; charset=us-ascii 6, 17 -- Date: Tue, 4 Mar 2014 13:49:04 -0500 6, 17 -- Subject: Announcing EBSD 2014 6, 17 -- To: microscopy-at-microscopy.com 6, 17 -- Message-Id: {0E6DA280-B299-463B-BF1E-56344F2B7337-at-umich.edu} 6, 17 -- Mime-Version: 1.0 (Mac OS X Mail 7.1 \(1827\)) 6, 17 -- X-Mailer: Apple Mail (2.1827) 6, 17 -- Content-Transfer-Encoding: 8bit 6, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s24InI94014552 ==============================End of - Headers==============================
Just another comment. Yes the window serves as a protective agent as outlined by others.
Although a TEM is not leaked in the same manner as some SEM's there is still inevitably a small burst of water vapor and gas that enters the column via the insertion via the airlock. So there will be a gradual but slow build up of condensate upon the cold detector, particularly if it is an older SiLi system cooled by LN2. Some older SiLi systems do have small heaters/thermal cycling modes to mitigate ice build up. If you have a SDD which is typically turned on/off then it will warm up on power down and any ice will sublime in vacuum.
What you don't want to happen is for the detector to be fully powered up and the column to be leaked to air. Not only will you get condensate, but more likely there will be a short circuit of the electrical connections at the back of the detector. The short circuit can destroy your detector.
Qualitative analysis will not be affected by a partially broken window, unless you get ice build up from water vapor in your column condensing on the front of the cold detector. This will decrease the detection efficiency for light elements. You will see this first on the low energy lines ( { 2 keV).
Please also remember since the presence or absence of a window affects the relative intensities of x-ray lines, a change in the window can impact any quantitative analyses. Literally all the quantitative analysis programs assume parameters for the initially installed window and the manufacturers will have this programmed into their analysis software.
A partially broken window will be difficult to model as you don't know how much of the detector area is affected. Depending upon the x-ray lines analyzed this can change your analysis, again low eneryg lines will be most affected.
So should you initially worry, I would not as long as the detector is still working. I have run windowless detectors (both LN2 cooled SiLi and SDD's) in TEM's for years. With proper care and attention they are fine.
However, if you are in a multi-user facility with variable expertise of users then I would play it safe and get the window repaired. Fixing a window is alot cheaper than replacing the whole crystal.
Nestor Your Friendly Neighborhood SysOp
On 3/4/14 10:33 AM, dcristofori-at-unive.it wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } if there's a hole in the window of an EDS detector mounted on a TEM, } what problem may arise using the detector? } I realizd that it's clear to me the window purpose in a SEM, where the } chamber is frequently vented, but what about in a TEM? } Thanks } } Davide } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive[dot]it } } Universitŕ Ca' Foscari Venezia } Dipartimento di Sc. Molecolari e Nanosistemi } Via Torino, 155b I-30172 Mestre (VE) Italy } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } } ==============================Original Headers============================== } 6, 20 -- From dcristofori-at-unive.it Tue Mar 4 10:33:15 2014 } 6, 20 -- Received: from orion.unive.it (orion.unive.it [157.138.1.25]) } 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s24GXFdq008244 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 10:33:15 -0600 } 6, 20 -- Received: by orion.unive.it (Postfix, from userid 498) } 6, 20 -- id 024D34275D; Tue, 4 Mar 2014 17:33:11 +0100 (CET) } 6, 20 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) } 6, 20 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) } 6, 20 -- (No client certificate requested) } 6, 20 -- by orion.unive.it (Postfix) with ESMTPSA id D661B42733 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 17:33:11 +0100 (CET) } 6, 20 -- Message-ID: {5316076C.6090101-at-unive.it} } 6, 20 -- Date: Tue, 04 Mar 2014 18:03:40 +0100 } 6, 20 -- From: Davide Cristofori {dcristofori-at-unive.it} } 6, 20 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 } 6, 20 -- MIME-Version: 1.0 } 6, 20 -- To: Microscopy-at-microscopy.com } 6, 20 -- Subject: TEM - EDS window purpose and holes } 6, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 6, 20 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
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Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Bldg 212 / A-143 9700 S. Cass Ave Argonne, Illinois 60439 USA
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Email: mlibbee-at-gmail.com Name: marissa
Organization: lbnl
Title-Subject: [Filtered] silicone removal
Message: Can someone recommend a chemical recipe to remove silicone from an LED package device?
Thank you
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Mar 5 07:58:15 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s25DwFH3010671 14, 28 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 07:58:15 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 68F616CB5DD 14, 28 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 07:58:15 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id LKA9C2dsSjmX for {microscopy-at-microscopy.com} ; 14, 28 -- Wed, 5 Mar 2014 07:58:06 -0600 (CST) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id A595F6CB5D0 14, 28 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 07:58:06 -0600 (CST) 14, 28 -- Message-ID: {53172D6E.7030402-at-microscopy.com} 14, 28 -- Date: Wed, 05 Mar 2014 07:58:06 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:LED silicone removal 14, 28 -- References: {201403041826.s24IQ5c8012188-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201403041826.s24IQ5c8012188-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201403041826.s24IQ5c8012188-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello Listservers, I am planning a freeze substituition exp on cultured cells that have been high pressure frozen for immunoelectron microscopy. I was wondering what the experts use as a transition/dehydration solvent for IEM, cold (-90C to -50c) methanol or acetone in their cocktail mixes. I have read where methanol causes more tissue shrinkage (rt) and cold acetone is better, although methanol may penetrate quicker. I am looking for acceptable labeling and ultrastructure. Any advice is appreciated.
Sincerely, Michael Delannoy Assoc. Director Johns Hopkins SOM Microscope Facility Baltimore, MD
==============================Original Headers============================== 3, 20 -- From prvs=13465c7fc=mdelann1-at-jhmi.edu Wed Mar 5 08:57:07 2014 3, 20 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.200]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s25Ev6nY031848 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 08:57:07 -0600 3, 20 -- X-IronPort-AV: E=Sophos;i="4.97,593,1389762000"; 3, 20 -- d="scan'208";a="352922958" 3, 20 -- Received: from bsno8925.ebalto.jhmi.edu (HELO BSNO8925) ([10.16.66.55]) 3, 20 -- by ipex1.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 05 Mar 2014 09:57:01 -0500 3, 20 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 3, 20 -- To: {Microscopy-at-microscopy.com} 3, 20 -- Subject: Methanol vs Acetone 3, 20 -- Date: Wed, 5 Mar 2014 09:57:01 -0500 3, 20 -- Message-ID: {002201cf3883$29d2b620$7d782260$-at-jhmi.edu} 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: text/plain; 3, 20 -- charset="us-ascii" 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-Mailer: Microsoft Outlook 14.0 3, 20 -- Thread-index: Ac84gnprNeS9MraHTgOZFVLKeLn3EQ== 3, 20 -- Content-Language: en-us ==============================End of - Headers==============================
From rotoowy-at-vieuxportdemontreal.com Wed Mar 5 09:56:03 2014 Return-Path: {rotoowy-at-vieuxportdemontreal.com} Received: from smtp1.vieuxportdemontreal.com ([109.127.80.19]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s25Fu1dI019177 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 5 Mar 2014 09:56:02 -0600 Message-Id: {201403051556.s25Fu1dI019177-at-ns.microscopy.com}
Marissa,
Silicones used in packaging of semiconductor devices are notoriously difficult to remove, but:
1) Hot red fuming HNO3 or hot 3:1 mix of red fuming HNO3 with fuming H2SO4 would work very nicely, if you do not mind loosing entire package and be left with bare LED die.
2) Soaking in warm (40C) Methylene Chloride overnight (under a fume hood) usually softens silicone to the point that it can be cleaned off by a Q-tip. Sonicating in Methylene Chloride may be even better, but I haven't try it. When using methylene chloride make sure it is dry - if contaminated by water it becomes corrosive.
3) I had limited success with sonicating parts in warm Dynasolve 230 (under a fume hood) for a few hours.
Good luck :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
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==============================Original Headers============================== 9, 39 -- From vray-at-partbeamsystech.com Wed Mar 5 10:23:17 2014 9, 39 -- Received: from nm10-vm0.bullet.mail.ne1.yahoo.com (nm10-vm0.bullet.mail.ne1.yahoo.com [98.138.91.72]) 9, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s25GNGTG019755 9, 39 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 10:23:16 -0600 9, 39 -- Received: from [98.138.100.118] by nm10.bullet.mail.ne1.yahoo.com with NNFMP; 05 Mar 2014 16:23:16 -0000 9, 39 -- Received: from [98.138.84.41] by tm109.bullet.mail.ne1.yahoo.com with NNFMP; 05 Mar 2014 16:23:16 -0000 9, 39 -- Received: from [127.0.0.1] by smtp109.mail.ne1.yahoo.com with NNFMP; 05 Mar 2014 16:23:16 -0000 9, 39 -- X-Yahoo-Newman-Id: 293852.72691.bm-at-smtp109.mail.ne1.yahoo.com 9, 39 -- X-Yahoo-Newman-Property: ymail-3 9, 39 -- X-YMail-OSG: 9vXK10oVM1mqgq_7VC1EtBb8MM0qSPRrLHZq58kEantcmk5 9, 39 -- DqM1DX7MSr99yG1nV80owx7LhlslzQoLBreYQnBEzTaAzNHi9xdCf3dzyFC4 9, 39 -- XwEuHkLLoZ0iaRVFfXz6X4NY1UvNcCl8ZjduTOWxSjts2esQxv1kAQSK3tHA 9, 39 -- fUZDTWCYw8HrFU5lYVUBOG1_VDfueWXGdxWQtZVIh8zS2DIMGNl5AC5sNvt0 9, 39 -- YV9RYeyWpHnrHHSaGzFMyPw.CpCxeWg.ej4GhObPn7kcyuDya70oBudAfY.s 9, 39 -- iaWM5dTpUv8FdFQNQOqEbA.6kXEkN2xuN87Hqdk3JkvItFcHVosTioSQxJsE 9, 39 -- eTnRo4_3i1RKsuptJljXBPd447WbsZJZQRSTsek.Rhk8fZK.iRXhAtNI_drJ 9, 39 -- m21XNY1grwu9yVug2Fa8yMS4s3Rn81cxrq2a3U1a4dBJ1PGe1Gf_xlwRaVgC 9, 39 -- QAqeqgUBE7vDwemBd.TIKBrwOMVI2DXCeE_rXf8pVc2ot7V6hcr5KFP8bPcx 9, 39 -- R854yptgM38zcnMwd7gJ.8xkhRvwy6b9UdMOZTa11BMWNyKb.DOAhGbphhjm 9, 39 -- SzUOTdP5iblDEAN9VDSkBggd.NAWh9rR46D9SxDiujiaXPnyGoFaIhq9_geu 9, 39 -- 4oZgHNi4DdXrbc3TZuICUkGq6IwdbPzAPmkfMk4GLpjm5D_5KIBcPkjPn1wH 9, 39 -- YjAN2yD_O385HXkKrSJ8ia4xy6AFdNr8HKGpXgKMhesPzuPFcq1lYM5VQmJ5 9, 39 -- IPpMLxeoS82ATyuon9d5UKzLMvLmUsxC.Q8hT6JmPIEzdQT2eSi5aAw-- 9, 39 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 9, 39 -- X-Rocket-Received: from [10.109.163.180] (vray-at-129.2.129.152 with plain [98.139.211.125]) 9, 39 -- by smtp109.mail.ne1.yahoo.com with SMTP; 05 Mar 2014 08:23:16 -0800 PST 9, 39 -- Message-ID: {53174F74.7040107-at-partbeamsystech.com} 9, 39 -- Date: Wed, 05 Mar 2014 11:23:16 -0500 9, 39 -- From: Valery Ray {vray-at-partbeamsystech.com} 9, 39 -- Reply-To: vray-at-partbeamsystech.com 9, 39 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 9, 39 -- MIME-Version: 1.0 9, 39 -- To: mlibbee-at-gmail.com 9, 39 -- CC: microscopy-at-microscopy.com 9, 39 -- Subject: Re: [Microscopy] viaWWW:LED silicone removal 9, 39 -- References: {201403051400.s25E0J2t011869-at-ns.microscopy.com} 9, 39 -- In-Reply-To: {201403051400.s25E0J2t011869-at-ns.microscopy.com} 9, 39 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, thank you for your replies and comments. They have been precious to me. Just to answer some questions in your comments: The detector is a Si(Li); we're going to repair the window (an upgrade to SDD was considered, but we'll wait for the moment - sigh), just wanted to have sounder idea of what would happen by operating the detector if any user of our facility asked me for it. Your answers clarified it. Thanks again
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universitŕ Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Il 04/03/2014 17.39, dcristofori-at-unive.it ha scritto: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } if there's a hole in the window of an EDS detector mounted on a TEM, } what problem may arise using the detector? } I realizd that it's clear to me the window purpose in a SEM, where the } chamber is frequently vented, but what about in a TEM? } Thanks } } Davide } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive[dot]it } } Universitŕ Ca' Foscari Venezia } Dipartimento di Sc. Molecolari e Nanosistemi } Via Torino, 155b I-30172 Mestre (VE) Italy } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } } ==============================Original Headers============================== } 6, 20 -- From dcristofori-at-unive.it Tue Mar 4 10:33:15 2014 } 6, 20 -- Received: from orion.unive.it (orion.unive.it [157.138.1.25]) } 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s24GXFdq008244 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 10:33:15 -0600 } 6, 20 -- Received: by orion.unive.it (Postfix, from userid 498) } 6, 20 -- id 024D34275D; Tue, 4 Mar 2014 17:33:11 +0100 (CET) } 6, 20 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) } 6, 20 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) } 6, 20 -- (No client certificate requested) } 6, 20 -- by orion.unive.it (Postfix) with ESMTPSA id D661B42733 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 17:33:11 +0100 (CET) } 6, 20 -- Message-ID: {5316076C.6090101-at-unive.it} } 6, 20 -- Date: Tue, 04 Mar 2014 18:03:40 +0100 } 6, 20 -- From: Davide Cristofori {dcristofori-at-unive.it} } 6, 20 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 } 6, 20 -- MIME-Version: 1.0 } 6, 20 -- To: Microscopy-at-microscopy.com } 6, 20 -- Subject: TEM - EDS window purpose and holes } 6, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 6, 20 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 22 -- From dcristofori-at-unive.it Wed Mar 5 11:17:35 2014 6, 22 -- Received: from orion.unive.it (orion.unive.it [157.138.1.25]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s25HHXvs007285 6, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 11:17:34 -0600 6, 22 -- Received: by orion.unive.it (Postfix, from userid 498) 6, 22 -- id 5ED1D4275E; Wed, 5 Mar 2014 18:17:29 +0100 (CET) 6, 22 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 6, 22 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 6, 22 -- (No client certificate requested) 6, 22 -- by orion.unive.it (Postfix) with ESMTPSA id 4B9094274B 6, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 18:17:29 +0100 (CET) 6, 22 -- Message-ID: {53176365.1090708-at-unive.it} 6, 22 -- Date: Wed, 05 Mar 2014 18:48:21 +0100 6, 22 -- From: Davide Cristofori {dcristofori-at-unive.it} 6, 22 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 6, 22 -- MIME-Version: 1.0 6, 22 -- To: Microscopy-at-microscopy.com 6, 22 -- Subject: Re: [Microscopy] TEM - EDS window purpose and holes 6, 22 -- References: {201403041639.s24GdhNV016509-at-ns.microscopy.com} 6, 22 -- In-Reply-To: {201403041639.s24GdhNV016509-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 6, 22 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From medications-popular7-at-xqqme.com Wed Mar 5 11:31:15 2014 Return-Path: {medications-popular7-at-xqqme.com} Received: from smtp3.xqqme.com ([31.29.198.219]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s25HVCKT023858 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 5 Mar 2014 11:31:13 -0600 Message-Id: {201403051731.s25HVCKT023858-at-ns.microscopy.com}
Dear List-Servers,
thank you for your suggestions and all your help! Sorry, that it took me so long to response. We were lucky and a FEI service-technician came this Monday. He found out that the problem was one of the valve, the NVC. So, currently I am working, quite happy, with stable 0.15 Torr (20 Pa) over the last 10h.
Thank you all again! Stefan
==============================Original Headers============================== 4, 32 -- From stefan.wernitznig-at-medunigraz.at Wed Mar 5 12:38:44 2014 4, 32 -- Received: from si068.medunigraz.at (si173.medunigraz.at [193.170.105.173]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s25IchOw027553 4, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 12:38:43 -0600 4, 32 -- X-AuditID: 0ac80c44-f79eb6d000000e72-0f-53176f31626a 4, 32 -- Received: from sj063.medunigraz.at (Unknown_Domain [10.200.18.79]) 4, 32 -- by si068.medunigraz.at (Symantec Mail Security) with SMTP id D8.EA.03698.13F67135; Wed, 5 Mar 2014 19:38:41 +0100 (CET) 4, 32 -- Received: from connect_63-MTA by sj063.medunigraz.at 4, 32 -- with Novell_GroupWise; Wed, 05 Mar 2014 19:38:41 +0100 4, 32 -- Message-Id: {53177CDA020000020001595A-at-sj063.medunigraz.at} 4, 32 -- X-Mailer: Novell GroupWise Internet Agent 12.0.2 4, 32 -- Date: Wed, 05 Mar 2014 19:36:58 +0100 4, 32 -- From: "Wernitznig Stefan" {stefan.wernitznig-at-medunigraz.at} 4, 32 -- To: {Microscopy-at-microscopy.com} 4, 32 -- Subject: Pressure stability problems in the low vacuum mode of an ESEM 4, 32 -- Mime-Version: 1.0 4, 32 -- Content-Type: text/plain; charset=US-ASCII 4, 32 -- Content-Disposition: inline 4, 32 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFtrNIsWRmVeSWpSXmKPExsXCdULIX9cwXzzYYP1WHYs/hxvYHRg9Pna/ 4, 32 -- ZgpgjOKySUnNySxLLdK3S+DKaL8XVfCbqWLnvEbWBsblTF2MHBwSAiYSO/bmdDFyApliEhfu 4, 32 -- rWfrYuTiEBLYyygx/WY7G0hCSCBX4vyJp8wgNq+AlcSVruWMEA2GEnf+TmYFmcMioCpxamck 4, 32 -- SJhNwFri1pmVbCBhEQF5ifn9YFOEBdwkbvz9BLaVV0BQ4u8OYZAws4CWxMNft1ggbG2JZQtf 4, 32 -- M4OUMAtISyz/xzGBkW8WQsMsJA2zkDTMQmhYwMiyilG4ONPAzEIvNzWlNC8zvSixSi+xZBMj 4, 32 -- OKx4XHYwNmwxPMQowMGoxMN7N1I8WIg1say4MvcQoyQHk5Ior10uUIgvKT+lMiOxOCO+qDQn 4, 32 -- tfgQowQHs5IIbwBIOW9KYmVValE+TEqag0VJnLcznD9YSCA9sSQ1OzW1ILUIJivDwaEkwXsa 4, 32 -- ZKhgUWp6akVaZk4JQpqJgxNkOA/Q8P0gNbzFBYm5xZnpEPlTjJJS4ry/QBICIImM0jy43leM 4, 32 -- 4kAvCPNeBsnyAFMEXNcroIFMQAOj+cAGliQipKQaGN3Cedaz/gt6w8X+wGiDSVU6uyz/Zimh 4, 32 -- sK3sH5ds0LOTf1ileei5m+ZNcafizqPNZta8m+tl71ltlPp3bZVGrtrD97/1ouZbGma2is82 4, 32 -- LFuc8v5vwsV5t9dm8H9Zu6VaOs1zkZRieLH3Itl11rJ+02Zy1safOen0Rp3DrvhpXWTXkmlz 4, 32 -- y16pK7EUZyQaajEXFScCAHMjHy7OAgAA 4, 32 -- Content-Transfer-Encoding: 8bit 4, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s25IchOw027553 ==============================End of - Headers==============================
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Email: levilr-at-ptd.net Name: Lee Levine
Organization: Process Solutions Consulting
Title-Subject: [Filtered] LED Silicone removal
Message: I use a solvent SU100 by R Moreau. Not the cleanest removal but it is as good as anything that I've found. It does swell the silicone possibly damaging wire bonds.
If you need to see the wire bonds do not use ultrasonic cleaners. They will make wire bonds fail in seconds.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Mar 5 18:05:36 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2605ZVR021582 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 18:05:36 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id EFDF36CBBA3 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 18:05:35 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id tzejI3uNzM-X for {microscopy-at-microscopy.com} ; 12, 28 -- Wed, 5 Mar 2014 18:05:34 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id C0FF06CBB96 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 18:05:34 -0600 (CST) 12, 28 -- Message-ID: {5317BBCE.9060708-at-microscopy.com} 12, 28 -- Date: Wed, 05 Mar 2014 18:05:34 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:LED Silicone removal 12, 28 -- References: {201403051710.s25HAsmo007129-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201403051710.s25HAsmo007129-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201403051710.s25HAsmo007129-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: What could cause our CM12 to turn on but then shut off a few seconds later? This EM was just re-assembled and I have just now been able to turn on the power. When I press the ON button it sounds as if it turns on. After a few seconds it shuts off and will only turn back on if unplugged then re-plugged back in. Does anyone have any insight on this?
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Mar 5 18:06:28 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2606RmZ022525 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 18:06:28 -0600 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id F20F36CBBB2 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 18:06:27 -0600 (CST) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id hd+BJB0cCJdk for {microscopy-at-microscopy.com} ; 12, 28 -- Wed, 5 Mar 2014 18:06:27 -0600 (CST) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id 4C2786CBBA5 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 18:06:27 -0600 (CST) 12, 28 -- Message-ID: {5317BC03.4000801-at-microscopy.com} 12, 28 -- Date: Wed, 05 Mar 2014 18:06:27 -0600 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:CM12 not staying on 12, 28 -- References: {201403051951.s25Jpr5c015354-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201403051951.s25Jpr5c015354-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201403051951.s25Jpr5c015354-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I'm not sure of the exact problem but as a first test, check the power supplies in the main power cabinet. They are behind the left door. There is a 24 volt and a 5 volt supply (perhaps some 122 volt supplies too). The power supplies have indicator LEDs on the front panel of the supply. Make sure those are powering up.
Cheers, Henk
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==============================Original Headers============================== 7, 49 -- From colijn.1-at-osu.edu Wed Mar 5 19:41:06 2014 7, 49 -- Received: from va3outboundpool.messaging.microsoft.com (va3ehsobe004.messaging.microsoft.com [216.32.180.14]) 7, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s261f5vi028019 7, 49 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 19:41:06 -0600 7, 49 -- Received: from mail109-va3-R.bigfish.com (10.7.14.245) by 7, 49 -- VA3EHSOBE007.bigfish.com (10.7.40.11) with Microsoft SMTP Server id 7, 49 -- 14.1.225.22; Thu, 6 Mar 2014 01:41:05 +0000 7, 49 -- Received: from mail109-va3 (localhost [127.0.0.1]) by 7, 49 -- mail109-va3-R.bigfish.com (Postfix) with ESMTP id 29137200BB for 7, 49 -- {microscopy-at-microscopy.com} ; Thu, 6 Mar 2014 01:41:05 +0000 (UTC) 7, 49 -- X-Forefront-Antispam-Report: CIP:164.107.81.212;KIP:(null);UIP:(null);IPV:NLI;H:cio-krc-pf05;RD:none;EFVD:NLI 7, 49 -- X-SpamScore: 2 7, 49 -- X-BigFish: VPS2(zzbb2dI62a3I98dI9371I1432Izz1f42h2148h1d77h208ch1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch1fc6hzd9hz1de098h177df4h17326ah8275bh1de097h186068hz2fh2a8h839h947hd25he5bhf0ah1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh162dh1631h1758h1765h18e1h190ch1946h19b4h19c3h19ceh1ad9h1b0ah224fh1d0ch1d2eh1d3fh1dfeh1dffh1f5fh1fe8h1ff5h209eh21e4h22d0h2336h2438h2461h2476h2487h24d7h2516h2545h255eh25cch25f6h2605h1b1cn1b1bi1155h) 7, 49 -- Received-SPF: pass (mail109-va3: domain of osu.edu designates 164.107.81.212 as permitted sender) client-ip=164.107.81.212; envelope-from=colijn.1-at-osu.edu; helo=cio-krc-pf05 ;cio-krc-pf05 ; 7, 49 -- Received: from mail109-va3 (localhost.localdomain [127.0.0.1]) by mail109-va3 7, 49 -- (MessageSwitch) id 1394070060799892_13270; Thu, 6 Mar 2014 01:41:00 +0000 7, 49 -- (UTC) 7, 49 -- Received: from VA3EHSMHS029.bigfish.com (unknown [10.7.14.240]) by 7, 49 -- mail109-va3.bigfish.com (Postfix) with ESMTP id AD867400047; Thu, 6 Mar 2014 7, 49 -- 01:41:00 +0000 (UTC) 7, 49 -- Received: from cio-krc-pf05 (164.107.81.212) by VA3EHSMHS029.bigfish.com 7, 49 -- (10.7.99.39) with Microsoft SMTP Server (TLS) id 14.16.227.3; Thu, 6 Mar 2014 7, 49 -- 01:41:00 +0000 7, 49 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) (using TLSv1 7, 49 -- with cipher RC4-MD5 (128/128 bits)) (No client certificate requested) by 7, 49 -- cio-krc-pf05 (Postfix) with ESMTPS id EF2B960059; Wed, 5 Mar 2014 20:40:59 7, 49 -- -0500 (EST) 7, 49 -- Received: from [192.168.1.102] (75.118.135.65) by smtp.service.osu.edu 7, 49 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.174.1; Wed, 5 Mar 7, 49 -- 2014 20:40:59 -0500 7, 49 -- Message-ID: {5317D226.1020807-at-osu.edu} 7, 49 -- Date: Wed, 5 Mar 2014 20:40:54 -0500 7, 49 -- From: Henk Colijn {colijn.1-at-osu.edu} 7, 49 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 7, 49 -- MIME-Version: 1.0 7, 49 -- To: {microscopy-at-microscopy.com} , 7, 49 -- "Schorp, Joshua C. (MU-Student)" 7, 49 -- {jcsmtf-at-mail.missouri.edu} 7, 49 -- Subject: Re: [Microscopy] viaWWW:CM12 not staying on 7, 49 -- References: {201403060007.s2607Qrn024985-at-ns.microscopy.com} 7, 49 -- In-Reply-To: {201403060007.s2607Qrn024985-at-ns.microscopy.com} 7, 49 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 7, 49 -- Content-Transfer-Encoding: 7bit 7, 49 -- X-Originating-IP: [75.118.135.65] 7, 49 -- X-CFilter-Loop: Forward 7, 49 -- X-FOPE-CRA-Verdict: 164.107.81.212$mail.missouri.edu%0%1%osu.edu%False%False%0$ 7, 49 -- X-OriginatorOrg: osu.edu 7, 49 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 7, 49 -- X-FOPE-CONNECTOR: Id%0$Dn%MAIL.MISSOURI.EDU$RO%1$TLS%0$FQDN%$TlsDn% ==============================End of - Headers==============================
From popular_medications13-at-achinsk.net Wed Mar 5 20:00:26 2014 Return-Path: {popular_medications13-at-achinsk.net} Received: from 46-43-216-31.achinsk.net (46-43-216-31.achinsk.net [46.43.216.31]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2620Nu4014620 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 5 Mar 2014 20:00:24 -0600 Message-Id: {201403060200.s2620Nu4014620-at-ns.microscopy.com}
Hi, Dan
This looks like an interesting design, if you have an existing spectrometer to slide it into.
Historically, I would have recommended the IlluminatIR, but it looks like it is no longer made by Smiths Detection.
I am at Pittcon this week and saw a brand new system called Spero, from Daylight Solutions (Google Daylight Solutions+Spero for their information). It was exceedingly clever in its design... everything was seamlessly integrated and the data output does not require highly trained staff to analyze. They have a live imaging mode for navigation and can collect a hyperspectral cube in record time.
Caveat: I have no commercial interest in any of these products.
Good hunting! Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through Fall 2014. Call us today for a free training evaluation.
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==============================Original Headers============================== 13, 36 -- From bfoster-at-the-mip.com Wed Mar 5 22:05:50 2014 13, 36 -- Received: from nm8-vm8.access.bullet.mail.bf1.yahoo.com (nm8-vm8.access.bullet.mail.bf1.yahoo.com [216.109.114.183]) 13, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2645lYI015866 13, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2014 22:05:47 -0600 13, 36 -- Received: from [66.196.81.165] by nm8.access.bullet.mail.bf1.yahoo.com with NNFMP; 06 Mar 2014 04:05:47 -0000 13, 36 -- Received: from [98.138.226.241] by tm11.access.bullet.mail.bf1.yahoo.com with NNFMP; 06 Mar 2014 04:05:47 -0000 13, 36 -- Received: from [127.0.0.1] by smtp112.sbc.mail.ne1.yahoo.com with NNFMP; 06 Mar 2014 04:05:46 -0000 13, 36 -- X-Yahoo-Newman-Id: 883962.72682.bm-at-smtp112.sbc.mail.ne1.yahoo.com 13, 36 -- Message-ID: {883962.72682.bm-at-smtp112.sbc.mail.ne1.yahoo.com} 13, 36 -- X-Yahoo-Newman-Property: ymail-3 13, 36 -- X-YMail-OSG: OInJGrYVM1kryulLes9x8vePmV5.l3zfj.2FmZky8bD8BlL 13, 36 -- epGaeRkHVY4YV1NPap2V_jRCC2We.yvDkYdqnv6mn0LPx5r5P8rFml0ukXmp 13, 36 -- pK6Tb9kQIkp2McnYF3O1E_bxCUwQbErs3va0MA6nSIXsm7Xj_Q70ThIu1H5d 13, 36 -- Ac9zkBuQruGac4VvG1Gw2PGfVnIfmiUIWNMbK5OzVs8iVcP6WqC.guJf_ZoR 13, 36 -- Z82.F8PsUnGMQAh6ZuWSwu3uAiXpWNRkiSBTP4Vr0iqIsFxZ1wpKa_AAnJOr 13, 36 -- .2z1wwOtnx0fmFrJ2EaBAqbC5mehRdnm_QjauBiAwpuAePZMei1ykLz8uXOa 13, 36 -- cYL7QVQIIQwLCOvH2BZprX4tRysObVlYAzq7EMoFXECnxaKHijpVLrYIAb.H 13, 36 -- XHDTrbkHDejaxT8QLpKjA_8cSjBLcum2t6aDFYpzVTJPBQYRNOGUKJzMXXGm 13, 36 -- ZdJT6vGGXSJEXUslM7oCrEKiPcRspd5tK6nbgpPHLZ8wGVn7nUcLYYJD52S4 13, 36 -- Aav_yz__HjFMONPT3u0oEEEV.VIJwNlYL4WfY66h43Dmd3MvDRhggBbAsfK1 13, 36 -- lOiYLyf1zsWy.6RN_Xwdl5atUqRj7fN5Dl6T7XwAVGkBTlsuoc50xpUVdRKj 13, 36 -- yswHoCtWx.CIWEVH2B9fxze0Hb5f2ziBfaBy1AxdiEgmgjNOShfIJWhm5CHS 13, 36 -- RsSNEmCI3tm.7v5s78Wm.3QuZKCawOZ4p1Z5CVh9_93ip3NSwFcgf4AAPs1a 13, 36 -- 6lF3q9LWUDug_m.Fh9u3YXQo0T6MYc.rpnIOLlAzFden1HVGvl_3Czq94KRR 13, 36 -- Mgd7R58iCRuNEh8oyhBXW5qgDZHG6845mXrgCm76.Ls0kwTEn65Q6MWXbIXv 13, 36 -- Dig-- 13, 36 -- X-Yahoo-SMTP: KOmzyESswBATZkB2214rZDIfSSNffJ2urJX_LO8NW63iukNVtw-- 13, 36 -- X-Rocket-Received: from Bs-PC.the-mip.com (bfoster-at-207.181.196.2 with plain [98.138.84.52]) 13, 36 -- by smtp112.sbc.mail.ne1.yahoo.com with SMTP; 05 Mar 2014 20:05:46 -0800 PST 13, 36 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 36 -- Date: Wed, 05 Mar 2014 22:05:43 -0600 13, 36 -- To: Microscopy-at-microscopy.com 13, 36 -- From: Barbara Foster {bfoster-at-the-mip.com} 13, 36 -- Subject: Fwd: [Microscopy] viaWWW:FTIR Microscope 13, 36 -- Mime-Version: 1.0 13, 36 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I can understand your skepticism, especially since I am a chemist by training. However, I spent considerable time while writing an early article on the IlluminatIR trying to figure out how to give microscopists an easy way to analyze spectra. From the demo I had today, these folks have solved that problem, at least with commonly available spectra. Of course, my mentor in this area, John Reffner, always advised collecting your own library, including the "sweepings from the corner of the lab."
My suggestion: get a demo and decide for yourself.
Good hunting! Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through Fall 2014. Call us today for a free training evaluation.
At 10:48 PM 3/5/2014, you wrote: } Re: "...the data output does not require highly } trained staff to analyze." } } Uh huh, sure. } } } -----Original Message----- } } From: bfoster-at-the-mip.com } } Sent: Mar 5, 2014 11:06 PM } } To: jtwilley-at-sprynet.com } } Subject: [Microscopy] Fwd: viaWWW:FTIR Microscope } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi, Dan } } } } This looks like an interesting design, if you have an existing } } spectrometer to slide it into. } } } } Historically, I would have recommended the IlluminatIR, but it looks } } like it is no longer made by Smiths Detection. } } } } I am at Pittcon this week and saw a brand new system called Spero, } } from Daylight Solutions (Google Daylight Solutions+Spero for their } } information). It was exceedingly clever in its design... everything } } was seamlessly integrated and the data output does not require highly } } trained staff to analyze. They have a live imaging mode for } } navigation and can collect a hyperspectral cube in record time. } } } } Caveat: I have no commercial interest in any of these products. } } } } Good hunting! } } Barbara Foster, President & Chief Consultant } } Microscopy/Microscopy Education* } } www.MicroscopyEducation.com } } } } *A subsidiary of The Microscopy & Imaging Place, Inc. } } 7101 Royal Glen Trail, Suite A } } McKinney, TX 75070 } } P: 972-924-5310 } } F: 214-592-0277 } } } } MME is currently scheduling courses for now and through Fall 2014. } } Call us today for a free training evaluation. } } } } } } } } } } } Envelope-to: bfoster-at-the-mip.com } } } Date: Mon, 3 Mar 2014 19:10:58 -0600 } } } To: bfoster-at-the-mip.com } } } From: microscopylistserver-noreply-at-microscopy.com } } } Reply-to: microscopylistserver-noreply-at-microscopy.com } } } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } } } } } } } } } } } } ------------------------------------------------------------------ } ---------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------ } ---------- } } } } } } X-from: dvanhart-at-binghamton.edu () } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } } --------------------------------------------------------------------------- } } } Remember this posting is most likely not from a Subscriber, so } when replying } } } please copy both dvanhart-at-binghamton.edu as well as the } } } Microscopy Listserver } } } --------------------------------------------------------------------------- } } } } } } Email: dvanhart-at-binghamton.edu } } } Name: Daniel VanHart } } } } } } Organization: Binghamton University } } } } } } Title-Subject: [Filtered] FTIR Microscope } } } } } } Message: Does anyone have any experience with a Pike Technologies } } } microMax Microscope? } } } } } } Login Host: 128.226.72.248 } } } Listserver Email Form V - 20120416 } } } --------------------------------------------------------------------------- } } } } } } } } } =========================================== } } } Do not reply to this message it is from } } } the Microscopy Listserver NO-REPLY forwarding } } } system. 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==============================Original Headers============================== 9, 42 -- From bfoster-at-the-mip.com Thu Mar 6 00:10:08 2014 9, 42 -- Received: from nm8-vm1.access.bullet.mail.gq1.yahoo.com (nm8-vm1.access.bullet.mail.gq1.yahoo.com [216.39.63.6]) 9, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s266A7rD003767 9, 42 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Mar 2014 00:10:08 -0600 9, 42 -- Received: from [216.39.60.176] by nm8.access.bullet.mail.gq1.yahoo.com with NNFMP; 06 Mar 2014 06:10:07 -0000 9, 42 -- Received: from [98.138.226.242] by tm12.access.bullet.mail.gq1.yahoo.com with NNFMP; 06 Mar 2014 06:10:07 -0000 9, 42 -- Received: from [127.0.0.1] by smtp113.sbc.mail.ne1.yahoo.com with NNFMP; 06 Mar 2014 06:10:07 -0000 9, 42 -- X-Yahoo-Newman-Id: 248040.70510.bm-at-smtp113.sbc.mail.ne1.yahoo.com 9, 42 -- Message-ID: {248040.70510.bm-at-smtp113.sbc.mail.ne1.yahoo.com} 9, 42 -- X-Yahoo-Newman-Property: ymail-3 9, 42 -- X-YMail-OSG: Sr8QEV4VM1mNR5RNIXgxnxS2iNCxhQmk3CjSCcPDijsng9R 9, 42 -- 5r0nZc8PalfyjB8Cd2EbfV6x0pHWozvJBM8XFQ7omadZiO9p8JsjhiFhOYY2 9, 42 -- 0w8lRFImuQlfo6tVcIudt3lGKjpPk2Tqi78Qzl.A.YKOkm65RbVweAZLI0hb 9, 42 -- QIR46KazeuI0fgJJoR8oMd9fxC7dJkv.Kfj2q5qH0vCVvK_2hTy3lNOB4Fxz 9, 42 -- WiPSrrQQ6wVXzfjM2jnUkSLRXZz15Q.Ahgg0LYPb9_EDZrkEoGbSCC64ZKxV 9, 42 -- vrY6t2KNvHXhxH56DcbouPrelNGf3V9TQ4lvp4KfIv.9apPJBH8dul_ZiWhg 9, 42 -- 3lF3O_bdn4QFMTMBdJ74EItqE_AB.05suQRE6jlX71lC1zPs51IJsc9sGRw3 9, 42 -- tTdHpyMO6LFFwVT4HXBmqPGMoj2Fpk5KWXFQluheLOSzT5dr2HZvuM0QKXwx 9, 42 -- yXLlzIKwvWsKqMft2oyCJlMljrVvz6HQurj2HexpzbHNf8JgIWHvxjeZ.X0R 9, 42 -- ZWhnRomAwn.XfFaV6abl3ux9gj9_psxaezTCwJE.CEFR64WIkdATKDSOfovK 9, 42 -- f77zUxdWiyG7jqGKTjZOrQLO_wsCUZ1fd8pZ0CtNQiuXuVul58.v75i7.H9L 9, 42 -- MqI1WaCJYzZISjrfxUeHAO81VRYbwsmnQOeXJIOnrTCO81l0v70FVZ5dagFt 9, 42 -- sswKALdLj3mfJAntr1C5j5wpYBY5XbUWroCvln6WHHY4oeVNTZ_.ggzGhOlr 9, 42 -- Ts6YsjSrwKGKJ3sM65ByeWKkDO49Pc.pSnFjKNmDoe12924uTQ6jj1ahSjaA 9, 42 -- fE1lfT67okYWLcsoXDkBQ8k5Fkw4iaEW4vQKKN5EzONDW3_QzxjR5wrOgXRu 9, 42 -- C8MA3iwpcYflo.rVTfjSztPhszH36tPq086xxncklfGYke3buNmmzvw1Cvhq 9, 42 -- 7MErMozf1fP4Uimr6erx5o785d2Omg4r51M3p6TKaa4dM38pRJnvuzjwcEzi 9, 42 -- m2elLgok1VgJ80RWX.q4FhQAI3K.beJ4Z6exNAPjtS.CAOrpSjhvFFjVTlkM 9, 42 -- nEjIEMykjKlr.8gEVSrkYqVk3IX4eKZo- 9, 42 -- X-Yahoo-SMTP: KOmzyESswBATZkB2214rZDIfSSNffJ2urJX_LO8NW63iukNVtw-- 9, 42 -- X-Rocket-Received: from Bs-PC.the-mip.com (bfoster-at-207.181.196.2 with plain [98.138.84.52]) 9, 42 -- by smtp113.sbc.mail.ne1.yahoo.com with SMTP; 06 Mar 2014 06:10:07 +0000 UTC 9, 42 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 42 -- Date: Thu, 06 Mar 2014 00:10:02 -0600 9, 42 -- To: jtwilley-at-sprynet.com, Microscopy-at-microscopy.com 9, 42 -- From: Barbara Foster {bfoster-at-the-mip.com} 9, 42 -- Subject: Re: [Microscopy] Fwd: viaWWW:FTIR Microscope 9, 42 -- In-Reply-To: {19616966.1394084103235.JavaMail.root-at-elwamui-lapwing.atl.s 9, 42 -- a.earthlink.net} 9, 42 -- References: {19616966.1394084103235.JavaMail.root-at-elwamui-lapwing.atl.sa.earthlink.net} 9, 42 -- Mime-Version: 1.0 9, 42 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
From medications-canadian9-at-nationalcablenetworks.ru Thu Mar 6 00:22:58 2014 Return-Path: {medications-canadian9-at-nationalcablenetworks.ru} Received: from broadband-178-140-53-161.nationalcablenetworks.ru (broadband-178-140-53-161.nationalcablenetworks.ru [178.140.53.161]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s266Mui4022856 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 6 Mar 2014 00:22:56 -0600 Message-Id: {201403060622.s266Mui4022856-at-ns.microscopy.com}
I've recently found a very nice parameterization of K-shell electron-impact ionization cross-sections (Talukder et al., Int. J. Mass Spec., V269 (2008) P. 118 and V309 (2012) P. 212) which are very convenient to code into the computer.
I'm not finding similar good (or even fair) parameterizations for L- or M-shells. Can anyone recommend good references from which to write code for X-ray cross-sections?
Thanks Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 7, 38 -- From parishcm-at-ornl.gov Thu Mar 6 09:26:39 2014 7, 38 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.14]) 7, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s26FQcfL030248 7, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Mar 2014 09:26:39 -0600 7, 38 -- X-SG: RELAYLIST 7, 38 -- X-IronPort-AV: E=Sophos;i="4.97,601,1389762000"; 7, 38 -- d="scan'208";a="77565475" 7, 38 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 7, 38 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 06 Mar 2014 10:26:38 -0500 7, 38 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151]) 7, 38 -- (using TLSv1 with cipher RC4-SHA (128/128 bits)) 7, 38 -- (No client certificate requested) 7, 38 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 273BC100230 7, 38 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Mar 2014 10:26:37 -0500 (EST) 7, 38 -- Received: from EXCHOS31.ornl.gov (128.219.12.151) by EXCHOS31.ornl.gov 7, 38 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.775.38; Thu, 6 Mar 7, 38 -- 2014 10:26:10 -0500 7, 38 -- Received: from exchhub2.ornl.gov (160.91.2.112) by EXCHOS31.ornl.gov 7, 38 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.775.38 via Frontend 7, 38 -- Transport; Thu, 6 Mar 2014 10:26:10 -0500 7, 38 -- Received: from EXCHMBB.ornl.gov ([192.168.3.201]) by exchhub2.ornl.gov 7, 38 -- ([160.91.2.112]) with mapi; Thu, 6 Mar 2014 10:26:10 -0500 7, 38 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 7, 38 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 7, 38 -- Date: Thu, 6 Mar 2014 10:26:08 -0500 7, 38 -- Subject: X-ray spectroscopy: L or M-shell parameterizations? 7, 38 -- Thread-Topic: X-ray spectroscopy: L or M-shell parameterizations? 7, 38 -- Thread-Index: Ac85UGT49TiRyO/aT8uejSCDewTBVA== 7, 38 -- Message-ID: {3C47B404D340EE48A469ADAE2ADBF9667DF5743E3A-at-EXCHMBB.ornl.gov} 7, 38 -- Accept-Language: en-US 7, 38 -- Content-Language: en-US 7, 38 -- X-MS-Has-Attach: 7, 38 -- X-MS-TNEF-Correlator: 7, 38 -- acceptlanguage: en-US 7, 38 -- Content-Type: text/plain; charset="us-ascii" 7, 38 -- MIME-Version: 1.0 7, 38 -- Content-Transfer-Encoding: 8bit 7, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s26FQcfL030248 ==============================End of - Headers==============================
We have a two-year postdoc position in atomic resolution STEM imaging (experiment, theory or both) at Monash University, Australia. The position is in collaboration with the Monash Centre for Electron Microscopy (inc. double-corrected Titan) and the theory group of Prof. Les Allen at the University of Melbourne. Further details below.
Please bring this position to the attention of anyone who might be interested.
Position description and application details available at: http://jobs.monash.edu.au/jobDetails.asp?sJobIDs=521173&lWorkTypeID=&lLocationID=&sJobNo=electron&lCategoryID=&lBrandID=&sKeywords=electron&stp=AW&sLanguage=en.
Position overview: We seek a postdoctoral researcher to conduct research in atomic resolution STEM imaging and spectroscopy. The postdoctoral researcher will work with Dr S.D. Findlay, A/Prof. M. Weyland, Dr A.J. D’Alfonso and Prof. L.J. Allen on atomic resolution imaging and spectroscopy on a project funded by the Australian Research Council. The position will be formally located in the School of Physics, Monash University, but will involve extensive collaboration with the Monash Centre for Electron Microscopy, and the Theoretical Condensed Matter Physics group at the University of Melbourne. The project will also involve collaborative work with colleagues in the UK, USA, Europe and Japan. The postdoctoral researcher will conduct research in experimental and/or theoretical aspects of atomic resolution electron microscopy at the highest international levels and translate research outcomes into high impact publications, present results at major conferences and assist in the supervision of PhD and final year undergraduate students.
Duration: Two-year appointment
Remuneration package: $79,485 - $85,323 pa Level A PhD / $91,609 - $108,788 pa Level B (inc 9.25% employer superannuation)
Closing date: Monday 31st March, 2014
Job No: 521173
Questions regarding the position can be directed to Dr Scott D. Findlay, scott.findlay-at-monash.edu, A/Prof. Matthew Weyland, matthew.weyland-at-monash.edu, Dr Adrian J. D'Alfonso, adrian-at-dalfonso.com.au, or Prof. Leslie J. Allen, lja-at-unimelb.edu.au.
-- Dr Scott Findlay QEII Fellow School of Physics Monash University Victoria, 3800, Australia Tel: +61 3 9902 4943 Fax: +61 3 9905 3637
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Title-Subject: [Filtered] Case for JEOL TEM Specimen Holder
Message: Does anyone know any vendor where I could purchase this case for a single tilt holder?
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Mar 6 22:07:55 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2747tm9013620 14, 28 -- for {microscopy-at-microscopy.com} ; Thu, 6 Mar 2014 22:07:55 -0600 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 65AD66CCAC1 14, 28 -- for {microscopy-at-microscopy.com} ; Thu, 6 Mar 2014 22:07:55 -0600 (CST) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id jAgS5UhjkZIF for {microscopy-at-microscopy.com} ; 14, 28 -- Thu, 6 Mar 2014 22:07:54 -0600 (CST) 14, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 50DF76CCAB4 14, 28 -- for {microscopy-at-microscopy.com} ; Thu, 6 Mar 2014 22:07:54 -0600 (CST) 14, 28 -- Message-ID: {53194619.5070407-at-microscopy.com} 14, 28 -- Date: Thu, 06 Mar 2014 22:07:53 -0600 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Case for JEOL TEM Specimen Holder 14, 28 -- References: {201403070119.s271JEGP011712-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201403070119.s271JEGP011712-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201403070119.s271JEGP011712-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From meds-popular14-at-starlink.ru Fri Mar 7 06:27:28 2014 Return-Path: {meds-popular14-at-starlink.ru} Received: from dhziel5-103.starlink.ru (dhziel5-103.starlink.ru [77.50.194.103] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s27CRPJ2006332 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 7 Mar 2014 06:27:26 -0600 Message-Id: {201403071227.s27CRPJ2006332-at-ns.microscopy.com}
Hello Listservers, What is the storage life of LN2 frozen AFS cocktails (0.2% glutaraldehyde, 0.25% uranyl acetate in 95% acetone) for HM20 IEM? Given that the glut stocks (10% acetone) when stored at 4C have a shelf life of 2 years.
Sincerely, Michael Delannoy
==============================Original Headers============================== 3, 20 -- From prvs=136010944=mdelann1-at-jhmi.edu Fri Mar 7 08:58:35 2014 3, 20 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.201]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s27EwZu6010256 3, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Mar 2014 08:58:35 -0600 3, 20 -- X-IronPort-AV: E=Sophos;i="4.97,608,1389762000"; 3, 20 -- d="scan'208";a="330886194" 3, 20 -- Received: from bsno8925.ebalto.jhmi.edu (HELO BSNO8925) ([10.16.66.55]) 3, 20 -- by ipex2.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 07 Mar 2014 09:58:35 -0500 3, 20 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 3, 20 -- To: {Microscopy-at-microscopy.com} 3, 20 -- Subject: Stored AFS cocktails 3, 20 -- Date: Fri, 7 Mar 2014 09:58:37 -0500 3, 20 -- Message-ID: {005101cf3a15$b83bba40$28b32ec0$-at-jhmi.edu} 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: text/plain; 3, 20 -- charset="us-ascii" 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-Mailer: Microsoft Outlook 14.0 3, 20 -- Thread-index: Ac86FTBu4TcxJqLIT1eNsKsEb4UzVg== 3, 20 -- Content-Language: en-us ==============================End of - Headers==============================
Rachel, The original stocks of 10% Ga in acetone where never opened (we use so little from each ampoule), I assume sealed under nitrogen. This solution polymerized in the bottles within 2 years (EMS confirmed 2 year shelf life at 4C). The working solutions (0.2% Ga 0.2% UA 95% acetone) were frozen and stored under LN2 conditions (-200C). I agree with you in the addition of 2-5% water to visualize membranes (bi-layer), and am curious about your IEM with 0.1% osmium. Do these sections need to be etched (saturated sodium-m-periodate) before labeling? Do you have a Ga Ua Osmium cocktail? What are the percentages?
Sincerely, Michael Delannoy
-----Original Message----- X-from: Reinhard Rachel [mailto:Reinhard.Rachel-at-biologie.uni-regensburg.de] Sent: Friday, March 07, 2014 10:50 AM To: Michael Delannoy
-----Original Message----- X-from: Reinhard Rachel [mailto:Reinhard.Rachel-at-biologie.uni-regensburg.de] Sent: Friday, March 07, 2014 10:50 AM To: mdelann1-at-jhmi.edu
X-from: robernal-at-u.northwestern.edu ()
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Title-Subject: [Filtered] Double Tilting Motor Electrical Diagram
Message: Hi,
The double tilt holders for JEOL usually have a standard connector that plugs into a terminal in the goniometer. In this way, one can control the secondary tilt from the microscope software. I am designing a custom double tilt holder and hope to make it motorized. Does anyone have the electrical diagram of this connector so that I can know how to wire my holder properly?
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Title-Subject: [Filtered] Faraday cup holder for Tecnai G2 F20
Message: Dear Listers,
I was wondering if someone has a Faraday Cup holder compatible Tecnai G2 F20 for a direct measurement of current on a specimen in STEM mode. We need to read the electron dose and dose rate, and our estimates seem to be off quite a bit.
If you know someone who knows someone, I would very much appreciate if you can refer me to this person. If you are the lucky owner, please contact me to discuss whether this could be arranged.
Emergent Atomic and Magnetic Structures Division of Materials Sciences and Engineering US DOE Ames Laboratory 332 Wilhelm Hall, Ames IA 50011 515-2943376 (office) 515-2941255 (lab) 515-294-8727 (fax) e-mail: tprozoro-at-ameslab.gov CREATING MATERIALS AND ENERGY SOLUTIONS
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Message: Our CM12 was at atmospheric pressure before turning on. When turned on, the prevac pumps down to P2=30. Then V10 will open, P2 will rise and slowly fall back down to 30. V2, V7, V5, V6, and ODP are all highlighted on the the screen. P1=35 and P3 will only go down to 66 after about 2 hours. The prevac turns on every 5 minutes or so and lowers P2 back down to 30 then shutting off, but once turned off, P2 goes up to 80. Is this correct, or do we have a problem with the ODP, leaks, etc.?
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Email: john.kourtesis-at-sars.uib.no Name: John
Organization: uniSAS, Bergen, norway
Title-Subject: [Filtered] LR White sectioning
Message: I have been facing difficulties in sectioning long consistent ribbons of 100nm thin sections of LR White biological tissue. Although I lower a lot the water on the trough and I use antistatic gun, the sections tend to submerge in the water
any hints, suggestions for more efficient ribbon sectioning?
many thanks
John
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Mar 8 16:31:16 2014 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s28MVGxv024064 15, 28 -- for {microscopy-at-microscopy.com} ; Sat, 8 Mar 2014 16:31:16 -0600 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id 11ADA6CE112 15, 28 -- for {microscopy-at-microscopy.com} ; Sat, 8 Mar 2014 16:31:16 -0600 (CST) 15, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id h2GiKandxOy6 for {microscopy-at-microscopy.com} ; 15, 28 -- Sat, 8 Mar 2014 16:31:10 -0600 (CST) 15, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id DD6ED6CE105 15, 28 -- for {microscopy-at-microscopy.com} ; Sat, 8 Mar 2014 16:31:10 -0600 (CST) 15, 28 -- Message-ID: {531B9A2E.3020306-at-microscopy.com} 15, 28 -- Date: Sat, 08 Mar 2014 16:31:10 -0600 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:LR White sectioning 15, 28 -- References: {201403081808.s28I8hYh015060-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201403081808.s28I8hYh015060-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201403081808.s28I8hYh015060-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From meds-cheapest8-at-mitchellcompanies.com Sat Mar 8 21:38:39 2014 Return-Path: {meds-cheapest8-at-mitchellcompanies.com} Received: from mail1.mitchellcompanies.com ([49.159.193.241]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s293cZCB015068 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 8 Mar 2014 21:38:36 -0600 Message-Id: {201403090338.s293cZCB015068-at-ns.microscopy.com}
Hi All,
Our software is not showing any x-ray counts and can't collect a spectrum when a sample is scanned.
We have a Bruker XFlash SDD detector set up on our JEOL SEM, with each device connected to it's own PC.
The PC's are connected to our network for internet access. Our IT department came through our lab the other day to address a network connectivity issue. After that, the Bruker Espirit software wasn't able to detect our X-Ray detector, an XFlash 4010. Turns out an ethernet cable going from the electronics signal processing box to the pc had been pulled. But even after reconnecting the software couldn't connect to the detector. We swapped cables, still no good. After a few days, we realized that the cable was a cross-patch cable, so we tried an ethernet cable out of the wall, and voila it reconnected to the detector and was getting counts.
But then after a session where it got reasonable counts, that matched previous scans of the same sample, the next day, no counts. The detector is recognized, Espirit can read some data from it (like the temperature of the detector), but it wouldn't register any counts.
We've checked the connections many times, especially because it looks like IT just pulled everything that looked like an ethernet cable. The Detector is connected to it's electronics box with a megalink cable, which seems securely in place, the electronics box is lighting up and indicating that data is being transferred, and the box is connected to the PC through a card which seems to be working properly otherwise (it accepts video from the SEM and that is picked up perfectly by the Espirit software, including operating conditions information, like voltage and magnification).
So it doesn't sound like there's any connection problem, but I have to wonder if the ethernet cable we've pulled out of the wall is really the same as what might be normally used for the electronics box to PC connection? They're both listed as Cat5e cables.
We've selected different throughput cards through the software, but still no counts. The detector hasn't been moved out of position, isn't touching the pole piece, and nothing seems to be blocking it. Bruker wants us to send in the detector and box, but they think it's likely that the detector itself is dead.
Has anyone had a similar problem and/or knows a resolution?
Our detector is around 7 years old, does that sound like a reasonable lifetime for a detector?
-- Robert J. Schenck Kingsborough Community College Physical Sciences Department Follow Me on Twitter: -at-Schenck
==============================Original Headers============================== 11, 26 -- From schenck.rob-at-gmail.com Sun Mar 9 11:25:06 2014 11, 26 -- Received: from mail-wg0-f51.google.com (mail-wg0-f51.google.com [74.125.82.51]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s29GP5J1011508 11, 26 -- for {Microscopy-at-Microscopy.com} ; Sun, 9 Mar 2014 11:25:06 -0500 11, 26 -- Received: by mail-wg0-f51.google.com with SMTP id k14so4971099wgh.34 11, 26 -- for {Microscopy-at-Microscopy.com} ; Sun, 09 Mar 2014 09:25:05 -0700 (PDT) 11, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 26 -- d=gmail.com; s=20120113; 11, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 11, 26 -- bh=pF4sJzYSDz0J465YcODUDYm6nqdrm+MRm1IpY78rBEs=; 11, 26 -- b=bOWowmI4bQae0tnc9n1n6fiqphLyXcFg1Vo9suQXpeRWLilFprn4hzCLtTOXVsIPXO 11, 26 -- 9msrQEp3EsquNe7CNAJjNNkVG5GqJFS9nJgKEkLJZtECDyMEFWO3MdFvGXOfFjSSrZTN 11, 26 -- qOvEfy1WrQTMBSzfU/+Equ3cEFa6u15gSaLNRFUklqjNNL6D9WKlznaiy4r1Q5f2D9Yf 11, 26 -- q3E8sPyxrAuZo2JWBnJgch6AIdbo8JeSWZPEGtq23PvN5stwoLsS792zMUck5RWBh/Mx 11, 26 -- zpjKxRAiW79G+ZYPBk/HwWWb9BMkRaToQgKnXrEegNZw2/aqF+5uiqrJwraIskkn5pgv 11, 26 -- Z4hg== 11, 26 -- MIME-Version: 1.0 11, 26 -- X-Received: by 10.194.6.8 with SMTP id w8mr28229341wjw.16.1394382305026; Sun, 11, 26 -- 09 Mar 2014 09:25:05 -0700 (PDT) 11, 26 -- Received: by 10.216.45.198 with HTTP; Sun, 9 Mar 2014 09:25:04 -0700 (PDT) 11, 26 -- Date: Sun, 9 Mar 2014 12:25:04 -0400 11, 26 -- Message-ID: {CAN=3F1jckiN8kq=F6cuA7sZCpOXV_EBm9T70WHDnhHGUhTtOdg-at-mail.gmail.com} 11, 26 -- Subject: [Microscopy] Bruker XFlash SDD X-ray detector no longer getting any counts 11, 26 -- From: Robert Schenck {schenck.rob-at-gmail.com} 11, 26 -- To: Microscopy-at-Microscopy.com 11, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Dear colleagues - my group is presently looking for postdoctoral candidates in the following areas: 1. In-situ oxide growth/atomically resolved STM/surface science characterization of fundamental physical and chemical phenomena on oxide surfaces 2. Dynamic SPM methods (multifrequency/band excitation) 3. Mesoscopic characterization of ferroelectric and energy storage and conversion materials All positions will utilize unique instrumental platforms developed at CNMS and will allow for significant independent program building. Please contact me directly for additional details. Sergei Kalinin
-- Sergei V. Kalinin
New: CNMS on FaceBook: https://www.facebook.com/CNMSORNL#!/CNMSORNL Google Scholar: http://scholar.google.com/citations?user=-cuxoSQAAAAJ&hl=en
Theme Leader for Electronic and Ionic Functionality The Center for Nanophase Materials Sciences and Materials Sciences and Technology Division Oak Ridge National Laboratory Oak Ridge, TN 37831
Joint Faculty Associate Professor, Bredesen Center for Interdisciplinary Research and Education University of Tennessee, Knoxville http://bredesencenter.utk.edu
Phone: (865) 241-0236 http://www.cnms.ornl.gov
==============================Original Headers============================== 7, 21 -- From sergei2-at-ornl.gov Mon Mar 10 09:31:59 2014 7, 21 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2AEVxRh017384 7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 10 Mar 2014 09:31:59 -0500 7, 21 -- X-SG: RELAYLIST 7, 21 -- X-IronPort-AV: E=Sophos;i="4.97,624,1389762000"; 7, 21 -- d="scan'208";a="55797481" 7, 21 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 7, 21 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 10 Mar 2014 10:31:59 -0400 7, 21 -- Received: from [128.219.192.92] (pc83680.ornl.gov [128.219.192.92]) 7, 21 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id E0DC2E1014; 7, 21 -- Mon, 10 Mar 2014 10:31:58 -0400 (EDT) 7, 21 -- Message-ID: {531DCCC1.7010700-at-ornl.gov} 7, 21 -- Date: Mon, 10 Mar 2014 10:31:29 -0400 7, 21 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 7, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:14.0) Gecko/20120713 Thunderbird/14.0 7, 21 -- MIME-Version: 1.0 7, 21 -- To: microscopy-at-microscopy.com, sv9-at-ornl.gov 7, 21 -- Subject: Postdoctoral positions -CNMS/ORNL 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From gkojaje-at-cds-engineering.com Tue Mar 11 02:13:51 2014 Return-Path: {gkojaje-at-cds-engineering.com} Received: from mail1.cds-engineering.com ([123.24.19.126]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2B7DkwB031588 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 11 Mar 2014 02:13:47 -0500 Message-Id: {201403110713.s2B7DkwB031588-at-ns.microscopy.com}
FYI
Inter/Micro 2014 June 2-6 at McCrone Research Institute in Chicago, Illinois McCrone Research Institute cordially invites you to participate in Inter/Micro 2014.
Papers are being solicited in micro-analytical techniques and instrumentation, environmental and industrial microscopy, and chemical and forensic microscopy.
The deadline to submit titles and abstracts is April 1, 2014.
For more information, visit our website at: www.mcri.org
Contact us at (312) 842-7100 or by email at: intermicro-at-mcri.org
We look forward to seeing you at Inter/Micro in Chicago!
McCrone Research Institute | 2820 S. Michigan Avenue | Chicago | IL | 60616
Tony ......................................... Andrew Anthony "Tony" Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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==============================Original Headers============================== 9, 27 -- From ph2-at-sprynet.com Tue Mar 11 23:44:13 2014 9, 27 -- Received: from elasmtp-curtail.atl.sa.earthlink.net (elasmtp-curtail.atl.sa.earthlink.net [209.86.89.64]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2C4iDVb003971 9, 27 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Mar 2014 23:44:13 -0500 9, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 27 -- s=dk20050327; d=sprynet.com; 9, 27 -- b=UZlYc2MbaAWUoKiHC17zqS6r6InunI1QCxOXAy1S2JquDIusjM2Zy6MYnTXchMV3; 9, 27 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language:X-ELNK-Trace:X-Originating-IP; 9, 27 -- Received: from [69.212.241.204] (helo=AAHHPdv7) 9, 27 -- by elasmtp-curtail.atl.sa.earthlink.net with esmtpa (Exim 4.67) 9, 27 -- (envelope-from {ph2-at-sprynet.com} ) 9, 27 -- id 1WNb1O-0005LT-JG 9, 27 -- for Microscopy-at-Microscopy.Com; Wed, 12 Mar 2014 00:44:02 -0400 9, 27 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 9, 27 -- To: "Microscopy Listserve" {Microscopy-at-Microscopy.Com} 9, 27 -- Subject: Inter/Micro 2014 Call for Papers - Extended Deadline 9, 27 -- Date: Wed, 12 Mar 2014 00:44:01 -0400 9, 27 -- Message-ID: {02a701cf3dad$b0e61920$12b24b60$-at-sprynet.com} 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="us-ascii" 9, 27 -- Content-Transfer-Encoding: 7bit 9, 27 -- X-Mailer: Microsoft Outlook 14.0 9, 27 -- Thread-Index: Ac89rT8hvdUGXoT9TP6BlkSQVYFnJQ== 9, 27 -- Content-Language: en-us 9, 27 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f965681d527e3357804e61a7533d0b0d2e350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 9, 27 -- X-Originating-IP: 69.212.241.204 ==============================End of - Headers==============================
From piiapei-at-bergstromauto.com Wed Mar 12 04:50:11 2014 Return-Path: {piiapei-at-bergstromauto.com} Received: from smtp1.bergstromauto.com (211.99.165.109.donpac.ru [109.165.99.211] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2C9o87M026721 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 12 Mar 2014 04:50:09 -0500 Message-Id: {201403120950.s2C9o87M026721-at-ns.microscopy.com}
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Just wanted to let you know about an upcoming webinar I invite you to join! Here are the details:
What: Webinar - Ensuring Color Integrity with Datacolor CHROMACAL - Learn about the Features and Benefits of this new product! When: Wednesday, April 2, 2014 1:00 PM – 2:00 PM EDT Who: Datacolor and Electron Microscopy Sciences Where to register: https://www2.gotomeeting.com/register/244965202 Additional information: Online Flyer: http://www.emsdiasum.com/microscopy/emailnews/chromacal_mar2014.html Website: http://www.emsdiasum.com/microscopy/products/calibration/systems.aspx or http://www.emsdiasum.com/chromacal Cost: free
Let me know if you have any questions! Thanks
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Mar 12 08:43:08 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2CDh7QG030606 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2014 08:43:07 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id ECD476D138C 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2014 08:43:07 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id QZdnK5PHaOFY for {microscopy-at-microscopy.com} ; 16, 28 -- Wed, 12 Mar 2014 08:43:06 -0500 (CDT) 16, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id DDA5C6D137A 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2014 08:43:06 -0500 (CDT) 16, 28 -- Message-ID: {5320646A.7020907-at-microscopy.com} 16, 28 -- Date: Wed, 12 Mar 2014 08:43:06 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:Webinar - Datacolor CHROMACAL 16, 28 -- References: {201403111809.s2BI9KvT009489-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201403111809.s2BI9KvT009489-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201403111809.s2BI9KvT009489-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 16, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Dear Colleagues, I should be glad if there are some dermatologist and skin researchers using the Microscopy Listserver who might find this information useful:
Announcement 41st Annual Meeting of the SCUR (Society for Cutaneous Ultrastructure Research ROME,ITALY; 2014) June 5-7,2014
1st Announcement 41st Annual Meeting of the SCUR (http://www.scur.org/content/e1174/e1181/e1182/index_ger.html)
"VILLA MONDRAGONE", Monte Porzio Catone (RM, ITALY) JUNE 5-7, 2014 GENERAL TOPIC: "SKIN AGEING and CANCER: From ULTRASTRUCTURE to CLINIC" INFORMATION (Flyer all forms)
Call for papers (in dermatology, dermatopathology, skin research, presenting of results from all ancient and modern imaging techniques)
Registration and Accommodation information available.
The deadline to submit titles and abstracts is March 20th, 2014. (late entries will be accepted on demand and need to be indicated by a mail to the Local Organizers at costanza-at-med.uniroma2.it; anapat-at-uniroma2.it; a.orlandi-at-uniroma2.it )
For more information, visit our website at: http://www.scur.org/content/e1163/e1164/index_ger.html (www.scur.org)
Contact us (in case of requests for special information)by email at: also: W.Muss-at-salk.at
We look forward to seeing you at the 41st Ann. Meeting of the SCUR in Monte Porzio Catone (RM), 20 km southeast of ROME city center (FRASCATI, near airport CIAMPINO or FIUMICINO), a marvelous place to stay!
Wolfgang Muss, PhD ......................................... SCUR Secretary Wolfgang MUSS EM-Lab, Univ. Inst. Pathology, SALK-LK (Gen. Hospital) and PMU (Private Paracelsus Medical University) Salzburg A-5020 SALZBURG-AUSTRIA
==============================Original Headers============================== 18, 38 -- From W.Muss-at-salk.at Wed Mar 12 09:24:23 2014 18, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 18, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2CEONfi017725 18, 38 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2014 09:24:23 -0500 18, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 18, 38 -- by hermes.salk.at (Postfix) with ESMTP id 01765646B33 18, 38 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2014 15:24:22 +0100 (CET) 18, 38 -- X-Scanned-By: SALK-Content-Filter 18, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 18, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 18, 38 -- with ESMTP id zxeXXdmv-T5e for {microscopy-at-microscopy.com} ; 18, 38 -- Wed, 12 Mar 2014 15:24:21 +0100 (CET) 18, 38 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 18, 38 -- by hermes.salk.at (Postfix) with ESMTP id D29BF646B2F 18, 38 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2014 15:24:21 +0100 (CET) 18, 38 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 18, 38 -- by N2EX199.lks.local with ESMTP; 12 Mar 2014 15:28:57 +0100 18, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 18, 38 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 18, 38 -- 14.02.0387.000; Wed, 12 Mar 2014 15:24:21 +0100 18, 38 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 18, 38 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 18, 38 -- Subject: Announcement: 41st Annual Meeting of the Society for Cutaneous 18, 38 -- Ultrastructure Research ROME,ITALY; June 5-7,2014 18, 38 -- Thread-Topic: Announcement: 41st Annual Meeting of the Society for Cutaneous 18, 38 -- Ultrastructure Research ROME,ITALY; June 5-7,2014 18, 38 -- Thread-Index: Ac89/sKG9kjZHKRBRui758eqwL7s5Q== 18, 38 -- Date: Wed, 12 Mar 2014 14:24:21 +0000 18, 38 -- Message-ID: {3D22E6EE65F39E448DCE4162815376711C67BBB2-at-N2DT131.lksdom21.lks.local} 18, 38 -- Accept-Language: de-DE, en-US 18, 38 -- Content-Language: de-DE 18, 38 -- X-MS-Has-Attach: 18, 38 -- X-MS-TNEF-Correlator: 18, 38 -- x-originating-ip: [192.168.42.2] 18, 38 -- Content-Type: text/plain; charset="iso-8859-1" 18, 38 -- MIME-Version: 1.0 18, 38 -- Content-Transfer-Encoding: 8bit 18, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2CEONfi017725 ==============================End of - Headers==============================
From drugs-cheapest4-at-quicktel.com Wed Mar 12 10:05:59 2014 Return-Path: {drugs-cheapest4-at-quicktel.com} Received: from smtp1.quicktel.com ([125.179.244.142]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2CF5ufn004786 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 12 Mar 2014 10:05:57 -0500 Message-Id: {201403121505.s2CF5ufn004786-at-ns.microscopy.com}
For those who are interested:
Many months ago I posted on list server asking for feed back as to which slow scan image capture system was the best. Thanks to all those who responded to both my enquiry and to past posts for the useful titbits. I should add I do not have shares in the company but am just a very satisfied customer. There was not much on these products on list server so I though I would just mention our deliberations.
Our short list was boiled down to 2 contenders either the quartz PCI system or the Saturn Imaging System (E.L.I. Orionmicroscopy.com) mainly I have to say based largely on cost.
For our users the main priority was ease of image capture and quality. Many of the other image capture systems we considered (not mentioned) put an over emphisis on post processing. We liked the simplcity of these two systems. Our users pay by the hour and do all the post processing on standalone workstations away from the microscope using open source or sitle licenced software.
We decided on the Saturn as it was not only very competively priced but offered active scan control of our FESEM (Jeol 6301F) which allows for much higher resolution scan than the default passive sync could allow (although it does have the passive modes also). It also allows for much clearer and noise free images. The layout is very simple and uncluttered and it allows the users to easily drive the SEM without fuss.
Given the age of the SEM it has given the machine a new lease of life and we have had no complaints from our varied users. We previously had a donated ADDAII which did not even come close with image quality.
In terms of PC it is a win7 box with no difficult cards inside so future maintainence should not be an issue. The electronics controller for the active scan is tiny and tucks away out of sight.
Jean Louis Leclef the proprieter designs, manufatures and installs the units himself and he was really optimised the system for us. We have ended up with a bespoke system that meets our requirements exactly. He even changes the software buttons to read out the way that suits the instrument.
Several desirable features have been incorportated for us which we find invaluable. In addition to the standard calibration linearisation methods he has implemented a method of calibrating images at all magnifications, probe currents, accelerating voltages, working distances etc so we can produce more accurate values for measuements. Our sem being vintage suffers from non linearilities and so this allows us to make very accurate measuments up to 500kx. He also has addaped the software to include a waveform display for live contrast/brightness adjustment and the system can do 0.2sec scans so you get an almost TV rate image on the PC. This is good for us as we have CRT screens on the SEM, these can be a bit noisy and weak with the Cold FEG.
The quality of the images is supurb, we have had no problems getting reliable publication quality images, from even the most inexperienced student. Users find the simple measurement tools and the way it stores data very conventient. All measurements are automatically exported with calibrated text files for imediate use, there is no need to batch export any more.
Jean Louis has given us great service support, every thing we wanted the software to do he has impliemented. We have had no problem with post instalation problems, these have been rectified within hours in most cases (most of these related to software alterations that we wanted not the hardware).
We aslo found this system was suitable to our STEM-TEM (Jeol 1200mk2) but we decided to upgrade the EDS system with image capture instead of this.
Should anyone require any futher information, images or comments please feel free to contact me off list. Like I say, I am not a sales rep just a very happy customer.
John Mitchels University of Bath, UK
==============================Original Headers============================== 14, 26 -- From john.mitchels-at-gmail.com Wed Mar 12 13:28:48 2014 14, 26 -- Received: from mail-ve0-f170.google.com (mail-ve0-f170.google.com [209.85.128.170]) 14, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2CISmCA015276 14, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Mar 2014 13:28:48 -0500 14, 26 -- Received: by mail-ve0-f170.google.com with SMTP id pa12so10866808veb.1 14, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Mar 2014 11:28:48 -0700 (PDT) 14, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 26 -- d=gmail.com; s=20120113; 14, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 14, 26 -- bh=D0XLRC7Ep3FvbYRi/C8Zd3eKEqpxD1qdfiW4oH2OI08=; 14, 26 -- b=M2Pvk2/H4k6JhEt24KTWvRQ7YAkGs2Dn359JZ0r1WnD/wdxDXwyZc6Az+DzO8K19cS 14, 26 -- 00nUgnBI2qDGkrNxj2SDO2u4ZjZQGE1OZXuFEtXq/XWFfQ2tzlDMnqiqNOGqqpUMUgFm 14, 26 -- b0O+Efc1bDDDSagTbmoSZABbOmtqhVI01iLpwpZLAfL9fzoSIdOUfmV2P5gTeh9O3fzK 14, 26 -- 7X1Gv59ghKGRjhPx9qZ0volILOodOGI5rd4L6aRa1JUpjIwTcSqAdoYTwDtGAxgoZYM4 14, 26 -- JT6HXPyZpG6eaTGBuEz/VXPZl2/STuCG+BNxTYVPo3qp+sirCk3swO7E6jxmO6AlC+ey 14, 26 -- g59g== 14, 26 -- MIME-Version: 1.0 14, 26 -- X-Received: by 10.221.34.211 with SMTP id st19mr33282020vcb.5.1394648927819; 14, 26 -- Wed, 12 Mar 2014 11:28:47 -0700 (PDT) 14, 26 -- Received: by 10.220.154.208 with HTTP; Wed, 12 Mar 2014 11:28:47 -0700 (PDT) 14, 26 -- Date: Wed, 12 Mar 2014 18:28:47 +0000 14, 26 -- Message-ID: {CAHX7yTQne9pgLzpm6--u06-8_9bLzFSjU=LF0dsaFruzyjMH6A-at-mail.gmail.com} 14, 26 -- Subject: Review of Saturn Active Image Capture System 14, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 14, 26 -- To: Microscopy-at-microscopy.com, Jean Louis Leclef {jl.leclef-at-gmail.com} 14, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
From meds-popular7-at-promtehnn.ru Thu Mar 13 13:11:30 2014 Return-Path: {meds-popular7-at-promtehnn.ru} Received: from mail.promtehnn.ru (mail.promtehnn.ru [94.100.84.86] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2DIBPv2030473 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 13 Mar 2014 13:11:27 -0500 Message-Id: {201403131811.s2DIBPv2030473-at-ns.microscopy.com}
Dear Listers,
For those of you doing color brightfield imaging, color integrity in microscopy will be part of MBL's Immunohistochemistry and Microscopy Workshop (March 15-20, Woods Hole, MA). Datacolor will have scientific staff supporting the workshop, discussing current trends in color integrity for microscopy and guiding hands-on lab exercises using the ChromaCal microscope calibration system to standardize color brightfield images and calibrate monitors for consistent, reliable color image viewing. For further details and a link to the workshop, visit http://scientific.datacolor.com/resources/events/
Best regards, Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through Fall 2014. Call us today for a free training evaluation.
==============================Original Headers============================== 6, 28 -- From bfoster-at-the-mip.com Thu Mar 13 14:55:38 2014 6, 28 -- Received: from barcelona.directrouter.com (barcelona.directrouter.com [72.249.107.2]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2DJtcOJ015436 6, 28 -- for {Microscopy-at-Microscopy.com} ; Thu, 13 Mar 2014 14:55:38 -0500 6, 28 -- Message-Id: {201403131955.s2DJtcOJ015436-at-ns.microscopy.com} 6, 28 -- Received: from 99-168-105-118.lightspeed.rcsntx.sbcglobal.net ([99.168.105.118]:57436 helo=Bs-PC.the-mip.com) 6, 28 -- by barcelona.directrouter.com with esmtpsa (TLSv1:DHE-RSA-AES256-SHA:256) 6, 28 -- (Exim 4.82) 6, 28 -- (envelope-from {bfoster-at-the-mip.com} ) 6, 28 -- id 1WOBj8-0001Im-H8 6, 28 -- for Microscopy-at-Microscopy.com; Thu, 13 Mar 2014 14:55:38 -0500 6, 28 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 28 -- Date: Thu, 13 Mar 2014 14:55:26 -0500 6, 28 -- To: Microscopy-at-Microscopy.com 6, 28 -- From: Barbara Foster {bfoster-at-the-mip.com} 6, 28 -- Subject: Color Integrity in Microscopy to be part of MBL's 6, 28 -- Immunohistochemistry/Microscopy Workshop 6, 28 -- Mime-Version: 1.0 6, 28 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 28 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 6, 28 -- X-AntiAbuse: Primary Hostname - barcelona.directrouter.com 6, 28 -- X-AntiAbuse: Original Domain - microscopy.com 6, 28 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 6, 28 -- X-AntiAbuse: Sender Address Domain - the-mip.com 6, 28 -- X-Get-Message-Sender-Via: barcelona.directrouter.com: authenticated_id: bfoster-at-the-mip.com 6, 28 -- X-Source: 6, 28 -- X-Source-Args: 6, 28 -- X-Source-Dir: ==============================End of - Headers==============================
We are interested in a short course that focuses on Electron Microscopy for cellular biology. Is there such a course? We have been to Lehigh but that focuses on Materials Science. We are particularly interested in sample preparation techniques: proper fixation, staining, microtome and then Cryo TEM to examine the samples.
Thanks,
Pete Eschbach Electron Microscope Facility Director 541 737 5645
Sent from my iPhone
==============================Original Headers============================== 5, 32 -- From peter.eschbach-at-comcast.net Thu Mar 13 17:31:25 2014 5, 32 -- Received: from qmta12.emeryville.ca.mail.comcast.net (qmta12.emeryville.ca.mail.comcast.net [76.96.27.227]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2DMVPIh004047 5, 32 -- for {microscopy-at-microscopy.com} ; Thu, 13 Mar 2014 17:31:25 -0500 5, 32 -- Received: from omta13.emeryville.ca.mail.comcast.net ([76.96.30.52]) 5, 32 -- by qmta12.emeryville.ca.mail.comcast.net with comcast 5, 32 -- id d70n1n00117UAYk01AXRjs; Thu, 13 Mar 2014 22:31:25 +0000 5, 32 -- Received: from [10.0.1.51] ([207.55.8.2]) 5, 32 -- by omta13.emeryville.ca.mail.comcast.net with comcast 5, 32 -- id dAXG1n00502dqGn8ZAXJSz; Thu, 13 Mar 2014 22:31:23 +0000 5, 32 -- Subject: EM techniques for cellular biology training? 5, 32 -- From: Peter Eschbach {peter.eschbach-at-comcast.net} 5, 32 -- Content-Type: text/plain; 5, 32 -- charset=us-ascii 5, 32 -- X-Mailer: iPhone Mail (11B554a) 5, 32 -- Message-Id: {DA5BDAA0-D4FF-4706-94EC-AA78911B6BE0-at-comcast.net} 5, 32 -- Date: Thu, 13 Mar 2014 15:31:16 -0700 5, 32 -- To: Microscopy-at-microscopy.com 5, 32 -- Mime-Version: 1.0 (1.0) 5, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 5, 32 -- s=q20121106; t=1394749885; 5, 32 -- bh=w72tXL3nDkc5lxZZ3wOWQJCg3WS9+KOgn2Sy7zllQt8=; 5, 32 -- h=Received:Received:Subject:From:Content-Type:Message-Id:Date:To: 5, 32 -- Mime-Version; 5, 32 -- b=C2C3hoQuxM12HnwHMpL5KgrDlAgAVDIn8QDWw48pJXqbUADkefeZ6ARHj0nzoe6kZ 5, 32 -- KAktumMjZz1qeqRg7u1FeXubsVFeHWtEbVOVj6PvTpGrMMPvdNBae5Ej4hFjMIH64O 5, 32 -- uDrVnvT13fUvtq4SaTGM/R2DiZIqRBf0dEBwKiAnHgfl25rKTpiEWR6sOfIEcoJ+7w 5, 32 -- /mcTkwt4+FQ7bs3OL+bHsOYQ//xvzYYBT/QxoUV/Wb5bLcDQy0MXhm3YwmWQnk7Ap4 5, 32 -- IHIZTQiUmgtAEOYn1VtuF104tDvLG9YSQkD5eaQdEVOhwW5LkBqI3ChcWdcXr49Y9z 5, 32 -- n9Bcw9Un56PDQ== 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2DMVPIh004047 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cgoldsmith-at-cdc.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] 50th anniversary meeting of the Southeastern Microscopy Society
Message: Join us in Columbia, SC, May 20 through 23 for the 2014 meeting of the Southeastern Microscopy Society! This will be the 50th Anniversary for the SEMS meeting and we are planning a large celebration and hope to see many friends and past members of SEMS, as well as welcoming many new members. Jay Jerome is the Program Chair for the meeting and many exciting talks and a strong Ruska competition for our students are being planned. Bob Price is the local arrangements chair and will be hosting a pre-meeting reception/fish fry at his home on the evening of May 20. All meeting participants are invited to attend.
The meeting will be held at the Clarion Hotel which is located at 1615 Gervais St in the downtown area of Columbia, SC. Visit our website at http://southeasternmicroscopy.org/2014-meeting for more information and a link to a 2-page flyer that will give additional information.
Please mark your calendars now for our 50th Anniversary meeting and help us make this milestone in the history of the Southeast Microscopy Society a meeting to remember!
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Mar 13 21:35:02 2014 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2E2Z2vO004023 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 13 Mar 2014 21:35:02 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id A1E266D2AB9 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 13 Mar 2014 21:35:02 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id vBgqwQpIQI4E for {microscopy-at-microscopy.com} ; 18, 28 -- Thu, 13 Mar 2014 21:35:01 -0500 (CDT) 18, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id E985B6D2AAC 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 13 Mar 2014 21:35:01 -0500 (CDT) 18, 28 -- Message-ID: {53226AD5.70909-at-microscopy.com} 18, 28 -- Date: Thu, 13 Mar 2014 21:35:01 -0500 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: viaWWW:50th anniversary meeting of the Southeastern Microscopy Society 18, 28 -- References: {201403132103.s2DL30qW002958-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201403132103.s2DL30qW002958-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201403132103.s2DL30qW002958-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We acquired a second-hand Robinson BSD that came with no paperwork. Can someone help me with a copy of the installation guide, operators manual and schematics for an older Robinson detector. I'm happy to pay copy costs and shipping.
Thanks, Owen
Owen P. Mills Director, Materials Characterization & Fabrication Facilities
Materials Science & Engineering Michigan Technological University 1400 Townsend Drive Houghton, MI 49931 PH 906-369-1875 FAX 906-487-2934
==============================Original Headers============================== 9, 17 -- From opmills-at-mtu.edu Sat Mar 15 10:15:17 2014 9, 17 -- Received: from email.mtu.edu (mx1.mtu.edu [141.219.73.15]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2FFFHti002361 9, 17 -- for {Microscopy-at-microscopy.com} ; Sat, 15 Mar 2014 10:15:17 -0500 9, 17 -- Received: from e12-0626-fac.coe.ad.mtu.edu (e12-0626-fac.coe.ad.mtu.edu [141.219.65.136]) 9, 17 -- by email.mtu.edu (Postfix) with ESMTPSA id ECD629CAEE 9, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Mar 2014 17:00:29 -0400 (EDT) 9, 17 -- From: "Owen P. Mills" {opmills-at-mtu.edu} 9, 17 -- Content-Type: text/plain; charset=us-ascii 9, 17 -- Subject: Manual for Robinson Backscatter Detector 9, 17 -- Message-Id: {952076C1-3BAD-4D06-BDB0-7F253792633B-at-mtu.edu} 9, 17 -- Date: Fri, 14 Mar 2014 17:00:21 -0400 9, 17 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 9, 17 -- Mime-Version: 1.0 (Mac OS X Mail 7.2 \(1874\)) 9, 17 -- X-Mailer: Apple Mail (2.1874) 9, 17 -- Content-Transfer-Encoding: 8bit 9, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2FFFHti002361 ==============================End of - Headers==============================
Just a short note that the Microscopy Listserver is back on-line.
Because of a Hacker & Denial_of_Service_Attack I temporarily shutdown all services on Friday.
If you were trying to get into any of the microscopy.com related sites, or even tried to send email tell me there was a problem, it did not get through.
No one got through my firewalls or to the listserver databases during the attack but I had soooooo much traffic coming in (thousands's of login attempts/minute) and warning logs flagging breaking attempts it was easier just to block the world with an iron fisted firewall for 24 hours.
BTW, the attack was out of BORANET in Korea / 203.248.143.169 and DATA Communication Bureau of Liaoning Province,China / 60.22.80.24 you might want to add them to your firewall black list if you have one.
Things should be back to normal now.
Nestor Your Friendly Neighborhood SysOp
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I need a little help with setting up my X-ray detector in Gatan’s Digital Micrograph DigiScan and DTSA-II programs. I have a JEOL 2100F with a High Resolution pole piece and with an Oxford Inca X-sight detector. Oxford would not give me the detector parameters that the programs need citing proprietary information. They sent me a Moxtek data sheet, but didn’t tell me which window it has, the AP3.3 or the AP3.7. Regardless, that information doesn’t help anyway, because Moxtek doesn’t give the parameters that the programs are asking for either. From my Oxford data sheet, it says that I have an ATW2 window.
Below are the parameters that I put into Digital Micrograph and DTSA. If you know the correct values or can help me figure out what they should be, please help. I don't know what the definition of the "zero fwhm" value is for the Gatan software is at all.
Detector: Dead layer: 100 nm (best guess) Active layer: 3e+006 nm (best guess is 3mm thick) Gold layer: 20 nm (best guess) Window thickness: 100 nm (Best guess) Window Type: Pyrolene (Best guess –the other choices are Aluminum, Boron Nitride, Diamond, and Hydrocarbon) Detector type: Si(Li) (finally, one I know for sure)
Characteristic Physics: Zero fwhm: 0.08049 (This value was already in DM EDS setup and I have no idea what should be or what it is. Anybody know? Fano: 0.1132 (This value was already in DM EDS setup, the theoretical value for Si is 0.115 from my search)
Here are the parameters that I used for DTSA: Window: Moxtek AP3.3 (It was the only Moxtek window definition from the data sheet from Oxford that I had) Elevation angle: 18.7° Azimuthal angle: 270° (I am taking the back of the TEM rod as the positive X-axis, i.e., 0°.) Optimal working distance: 6.39 mm (I took the sample-to-detector distance *sin(18.7°) Sample-to-detector distance: 19.927 mm (from the Oxford drawing sheet for the HR pole piece)
Crystal Parameters: Detector area:30 mm^2 (known) Gold layer: 8 nm (This is DTSA default value. Good as any that I know of.) Dead Layer: 0.085 (This is DTSA default value. Good as any that I know of.) Thickness: 3mm (The default for DTSA was 5, but I chose 3 mm. The intensity of the higher energy peaks on this detector seem to be low so I chose the lower number. Again, Best guess.) Resolution: 129 eV (This was recently measured by Oxford on a PM visit.)
==============================Original Headers============================== 8, 37 -- From s.walck-at-comcast.net Sat Mar 15 21:32:42 2014 8, 37 -- Received: from qmta02.westchester.pa.mail.comcast.net (qmta02.westchester.pa.mail.comcast.net [76.96.62.24]) 8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2G2WfAd001948 8, 37 -- for {microscopy-at-microscopy.com} ; Sat, 15 Mar 2014 21:32:41 -0500 8, 37 -- Received: from omta05.westchester.pa.mail.comcast.net ([76.96.62.43]) 8, 37 -- by qmta02.westchester.pa.mail.comcast.net with comcast 8, 37 -- id e2Az1n0010vyq2s512YhQc; Sun, 16 Mar 2014 02:32:41 +0000 8, 37 -- Received: from resmail-ch2-280v.sys.comcast.net ([162.150.49.59]) 8, 37 -- by omta05.westchester.pa.mail.comcast.net with comcast 8, 37 -- id e2Yh1n0021GdifW3R2Yh3U; Sun, 16 Mar 2014 02:32:41 +0000 8, 37 -- Date: Sun, 16 Mar 2014 02:32:40 +0000 (UTC) 8, 37 -- From: "S.Walck" {s.walck-at-comcast.net} 8, 37 -- To: MicroscopyListserver {Microscopy-at-microscopy.com} , 8, 37 -- microscopy-at-microscopy.org 8, 37 -- Message-ID: {9928331.9933282.1394937160953.JavaMail.root-at-comcast.net} 8, 37 -- In-Reply-To: {1979810856.9932946.1394936930054.JavaMail.root-at-comcast.net} 8, 37 -- Subject: JEOL 2100F/Oxford X-ray detector configuration for Gatan Digiscan 8, 37 -- and NIST DTSA-II 8, 37 -- MIME-Version: 1.0 8, 37 -- Content-Type: text/plain; charset=utf-8 8, 37 -- X-Originating-IP: [2601:a:80:39b:f1ea:e8f:7a45:7a5] 8, 37 -- X-Mailer: Zimbra 8.0.3_GA_5664 (ZimbraWebClient - IE9 (Win)/8.0.3_GA_5664) 8, 37 -- Thread-Topic: JEOL 2100F/Oxford X-ray detector configuration for Gatan Digiscan and NIST DTSA-II 8, 37 -- Thread-Index: PUHT/lHUftWL9IzlFCSvTsfbGopeEg== 8, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 8, 37 -- s=q20140121; t=1394937161; 8, 37 -- bh=1SA59hnEqMfuNkJzDwCbS6Yt5BDsNW8RsdiOd1Yx2Rs=; 8, 37 -- h=Received:Received:Date:From:To:Message-ID:Subject:MIME-Version: 8, 37 -- Content-Type; 8, 37 -- b=XHLWpyQld1rXUvqNA6LwTEb5ORmlRwDgutLDVFIJYclnm7ISZN9pThnxFgCH/b0Yt 8, 37 -- 2Lsh8W4sQqs6Xc4k5+FmkgS7Jb4J3ZYV/Ssb7vY7AMvANQsk/yOKgCni5ZbpnRuQzR 8, 37 -- rJ7pm3m+HanlgG6JqYN+mnnKOfmbteEYQNyMUAzicR3WvVF8TDuwFdEsOy8P6vPUQG 8, 37 -- M2hWpp9KAD5u8hksEoWEVaEuG+d/yoiAZVgswdqkSW3iksdmYH+YUgsCxfk00oqyom 8, 37 -- OT+cVXTyZ40VWFvURPLghmVRUqVRHjqMrwKrPTUsrYkR/Y7RZQ/lAEitq10i4Jg75N 8, 37 -- hvxzjliiO+DjQ== 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2G2WfAd001948 ==============================End of - Headers==============================
I wanted to share a posting by John Oreopoulos to the confocal listserv. Tom Phillips
Begin forwarded message: X-from: John Oreopoulos {john.oreopoulos-at-UTORONTO.CA}
Tom -
For a 36 page detailed PDF about this scope, see http://arxiv.org/abs/1403.1211 The 50 cent cost of the scope is based on the cost of components for 10,000 of them.
Caroline -- Caroline Schooley Project MICRO Co-Chair Microscopy Society of America 45301 Caspar Point Road, Box 117 Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/education/projectMICRO
==============================Original Headers============================== 3, 18 -- From schooley-at-mcn.org Sun Mar 16 13:07:37 2014 3, 18 -- Received: from smtp1.mcn.org (smtp1.mcn.org [216.150.240.85]) 3, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2GI7arv021721 3, 18 -- for {microscopy-at-microscopy.com} ; Sun, 16 Mar 2014 13:07:37 -0500 3, 18 -- Received: from [66.81.52.37] 3, 18 -- by smtp1.mcn.org with esmtpa (Exim 4.82) 3, 18 -- (envelope-from {schooley-at-mcn.org} ) 3, 18 -- id 1WPFTA-0005fj-6K; Sun, 16 Mar 2014 11:07:33 -0700 3, 18 -- Mime-Version: 1.0 3, 18 -- Message-Id: {a06240802cf4b95d5a02e-at-[66.81.53.223]} 3, 18 -- In-Reply-To: {201403161609.s2GG9Kgn007723-at-ns.microscopy.com} 3, 18 -- References: {201403161609.s2GG9Kgn007723-at-ns.microscopy.com} 3, 18 -- Date: Sun, 16 Mar 2014 11:06:37 -0700 3, 18 -- To: PhillipsT-at-missouri.edu 3, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 3, 18 -- Subject: Re: [Microscopy] FW: A paper microscope? 3, 18 -- Cc: microscopy-at-microscopy.com 3, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
In my previous post, part of my question asked what the term "zero fwhm" term was in the Gatan Digital Micrograph software. Â
I'd like to verify it is essentially the Noise value in the following expression for the resolution at a given X-ray energy,
FWHM = SQRT[ Noise^2 + 2.35^2 * epsilon * F & E], where epsilon is 3.8 eV for a Si detector, F is the Fano factor, ~0.115, and E is the energy of the X-ray. Â For my detector, the resolution for Mn-Ka was measured to be 129 eV, so I calculated the "Noise" value to be 49 eV. Â I assume that this is the correct number to put into the Gatan software.
Please comment if I am incorrect.
Thanks.
-Scott Walck
==============================Original Headers============================== 9, 36 -- From s.walck-at-comcast.net Sun Mar 16 21:48:10 2014 9, 36 -- Received: from qmta02.westchester.pa.mail.comcast.net (qmta02.westchester.pa.mail.comcast.net [76.96.62.24]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2H2m9s5016815 9, 36 -- for {microscopy-at-microscopy.com} ; Sun, 16 Mar 2014 21:48:10 -0500 9, 36 -- Received: from omta04.westchester.pa.mail.comcast.net ([76.96.62.35]) 9, 36 -- by qmta02.westchester.pa.mail.comcast.net with comcast 9, 36 -- id eSPh1n0020ldTLk51So98S; Mon, 17 Mar 2014 02:48:09 +0000 9, 36 -- Received: from resmail-ch2-280v.sys.comcast.net ([162.150.49.59]) 9, 36 -- by omta04.westchester.pa.mail.comcast.net with comcast 9, 36 -- id eSo91n00M1GdifW01So9cR; Mon, 17 Mar 2014 02:48:09 +0000 9, 36 -- Date: Mon, 17 Mar 2014 02:48:09 +0000 (UTC) 9, 36 -- From: "S.Walck" {s.walck-at-comcast.net} 9, 36 -- To: MicroscopyListserver {microscopy-at-microscopy.com} 9, 36 -- Message-ID: {694940283.10280428.1395024489429.JavaMail.root-at-comcast.net} 9, 36 -- In-Reply-To: {1902493593.10215764.1395008371979.JavaMail.root-at-comcast.net} 9, 36 -- References: {1902493593.10215764.1395008371979.JavaMail.root-at-comcast.net} 9, 36 -- Subject: zero fwhm value for Gatan's DigiScan software 9, 36 -- MIME-Version: 1.0 9, 36 -- Content-Type: text/plain; charset=utf-8 9, 36 -- X-Originating-IP: [2601:a:80:39b:4142:fb04:3867:492] 9, 36 -- X-Mailer: Zimbra 8.0.3_GA_5664 (ZimbraWebClient - IE9 (Win)/8.0.3_GA_5664) 9, 36 -- Thread-Topic: zero fwhm value for Gatan's DigiScan software 9, 36 -- Thread-Index: 8r0nEVNA8Ep9ufh9SUxEskW4lgdcD/AqEJJH 9, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 9, 36 -- s=q20140121; t=1395024489; 9, 36 -- bh=fQCwnZ6XoR39B8SMWjAT/Byw85hYxePnW76X5RPDOEQ=; 9, 36 -- h=Received:Received:Date:From:To:Message-ID:Subject:MIME-Version: 9, 36 -- Content-Type; 9, 36 -- b=KpnJ+3ql9kmi63Uee8FiiM0qtpuxvMMSC3ZqDoF8w7+FdxShk3sSGQ3y/9oAinVIJ 9, 36 -- KWjNbxpt4XgSONqqZ3hGQoSfD4WwN4/gznhfTUHyP9kg8IwvoWfYRvvFaJnnIQClkl 9, 36 -- QVHBBYCoDzCqEJz1AGZ+4twIGxCLW576+Z4LD+D7JKf7RvudAjec0J4izPj8zLTrGz 9, 36 -- 6NAyWmMlYJas9fm64l/9Dp9CI+AMNqQDMYa+/QWQJAe5bOj6d9EOakhTyuSj/Zn30C 9, 36 -- GFTAl80pd7wLQjrwIHwXI1G+gZE72dxKnTld+m8M7q/fwwYeHqfDsiue6CYvtF7MWT 9, 36 -- fZsmDTqx9zQ8g== 9, 36 -- Content-Transfer-Encoding: 8bit 9, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2H2m9s5016815 ==============================End of - Headers==============================
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From medications-discounted2-at-mgts.by Mon Mar 17 07:28:20 2014 Return-Path: {medications-discounted2-at-mgts.by} Received: from mm-120-37-120-178.dynamic.pppoe.mgts.by ([178.122.212.88]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2HCSIAF032197 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 17 Mar 2014 07:28:18 -0500 Message-Id: {201403171228.s2HCSIAF032197-at-ns.microscopy.com}
Scott; Someone from Oxford once told me that there is no gold layer on their detectors-they use nickel.
John Mardinly, ASU
I need a little help with setting up my X-ray detector in Gatan’s Digital Micrograph DigiScan and DTSA-II programs. I have a JEOL 2100F with a High Resolution pole piece and with an Oxford Inca X-sight detector. Oxford would not give me the detector parameters that the programs need citing proprietary information. They sent me a Moxtek data sheet, but didn’t tell me which window it has, the AP3.3 or the AP3.7. Regardless, that information doesn’t help anyway, because Moxtek doesn’t give the parameters that the programs are asking for either. From my Oxford data sheet, it says that I have an ATW2 window.
Below are the parameters that I put into Digital Micrograph and DTSA. If you know the correct values or can help me figure out what they should be, please help. I don't know what the definition of the "zero fwhm" value is for the Gatan software is at all.
Detector: Dead layer: 100 nm (best guess) Active layer: 3e+006 nm (best guess is 3mm thick) Gold layer: 20 nm (best guess) Window thickness: 100 nm (Best guess) Window Type: Pyrolene (Best guess –the other choices are Aluminum, Boron Nitride, Diamond, and Hydrocarbon) Detector type: Si(Li) (finally, one I know for sure)
Characteristic Physics: Zero fwhm: 0.08049 (This value was already in DM EDS setup and I have no idea what should be or what it is. Anybody know? Fano: 0.1132 (This value was already in DM EDS setup, the theoretical value for Si is 0.115 from my search)
Here are the parameters that I used for DTSA: Window: Moxtek AP3.3 (It was the only Moxtek window definition from the data sheet from Oxford that I had) Elevation angle: 18.7° Azimuthal angle: 270° (I am taking the back of the TEM rod as the positive X-axis, i.e., 0°.) Optimal working distance: 6.39 mm (I took the sample-to-detector distance *sin(18.7°) Sample-to-detector distance: 19.927 mm (from the Oxford drawing sheet for the HR pole piece)
Crystal Parameters: Detector area:30 mm^2 (known) Gold layer: 8 nm (This is DTSA default value. Good as any that I know of.) Dead Layer: 0.085 (This is DTSA default value. Good as any that I know of.) Thickness: 3mm (The default for DTSA was 5, but I chose 3 mm. The intensity of the higher energy peaks on this detector seem to be low so I chose the lower number. Again, Best guess.) Resolution: 129 eV (This was recently measured by Oxford on a PM visit.)
==============================Original Headers============================== 8, 37 -- From s.walck-at-comcast.net Sat Mar 15 21:32:42 2014 8, 37 -- Received: from qmta02.westchester.pa.mail.comcast.net (qmta02.westchester.pa.mail.comcast.net [76.96.62.24]) 8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2G2WfAd001948 8, 37 -- for {microscopy-at-microscopy.com} ; Sat, 15 Mar 2014 21:32:41 -0500 8, 37 -- Received: from omta05.westchester.pa.mail.comcast.net ([76.96.62.43]) 8, 37 -- by qmta02.westchester.pa.mail.comcast.net with comcast 8, 37 -- id e2Az1n0010vyq2s512YhQc; Sun, 16 Mar 2014 02:32:41 +0000 8, 37 -- Received: from resmail-ch2-280v.sys.comcast.net ([162.150.49.59]) 8, 37 -- by omta05.westchester.pa.mail.comcast.net with comcast 8, 37 -- id e2Yh1n0021GdifW3R2Yh3U; Sun, 16 Mar 2014 02:32:41 +0000 8, 37 -- Date: Sun, 16 Mar 2014 02:32:40 +0000 (UTC) 8, 37 -- From: "S.Walck" {s.walck-at-comcast.net} 8, 37 -- To: MicroscopyListserver {Microscopy-at-microscopy.com} , 8, 37 -- microscopy-at-microscopy.org 8, 37 -- Message-ID: {9928331.9933282.1394937160953.JavaMail.root-at-comcast.net} 8, 37 -- In-Reply-To: {1979810856.9932946.1394936930054.JavaMail.root-at-comcast.net} 8, 37 -- Subject: JEOL 2100F/Oxford X-ray detector configuration for Gatan Digiscan 8, 37 -- and NIST DTSA-II 8, 37 -- MIME-Version: 1.0 8, 37 -- Content-Type: text/plain; charset=utf-8 8, 37 -- X-Originating-IP: [2601:a:80:39b:f1ea:e8f:7a45:7a5] 8, 37 -- X-Mailer: Zimbra 8.0.3_GA_5664 (ZimbraWebClient - IE9 (Win)/8.0.3_GA_5664) 8, 37 -- Thread-Topic: JEOL 2100F/Oxford X-ray detector configuration for Gatan Digiscan and NIST DTSA-II 8, 37 -- Thread-Index: PUHT/lHUftWL9IzlFCSvTsfbGopeEg== 8, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 8, 37 -- s=q20140121; t=1394937161; 8, 37 -- bh=1SA59hnEqMfuNkJzDwCbS6Yt5BDsNW8RsdiOd1Yx2Rs=; 8, 37 -- h=Received:Received:Date:From:To:Message-ID:Subject:MIME-Version: 8, 37 -- Content-Type; 8, 37 -- b=XHLWpyQld1rXUvqNA6LwTEb5ORmlRwDgutLDVFIJYclnm7ISZN9pThnxFgCH/b0Yt 8, 37 -- 2Lsh8W4sQqs6Xc4k5+FmkgS7Jb4J3ZYV/Ssb7vY7AMvANQsk/yOKgCni5ZbpnRuQzR 8, 37 -- rJ7pm3m+HanlgG6JqYN+mnnKOfmbteEYQNyMUAzicR3WvVF8TDuwFdEsOy8P6vPUQG 8, 37 -- M2hWpp9KAD5u8hksEoWEVaEuG+d/yoiAZVgswdqkSW3iksdmYH+YUgsCxfk00oqyom 8, 37 -- OT+cVXTyZ40VWFvURPLghmVRUqVRHjqMrwKrPTUsrYkR/Y7RZQ/lAEitq10i4Jg75N 8, 37 -- hvxzjliiO+DjQ== 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2G2WfAd001948 ==============================End of - Headers==============================
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Greetings All,
The Midwest Microscopy and Microanalysis Society will hold its Spring meeting on Friday, March 28, 2014, at Northwestern University in Evanston, IL. The topic is Innovative Biological Microscopy, and details can be found on our website under Meetings:
http://www.midwestmicroscopy.org/
Please join us for an interesting and informative program and a chance to meet with your local microscopy colleagues. We look forward to seeing you there!
If you visit our website, you'll also find the latest edition of our newsletter, with a review of last year's meetings, 2014 meeting dates, links to our new social media sites and information about our 2014 Student Travel Awards for attendance at M&M 2014/IUMAS-6 in Hartford, CT.
Best regards,
Elaine Schumacher M3S Program Coordinator
********************************************************************* Elaine F. Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
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Email: andre.dufresne-at-umanitoba.ca Name: Andre Dufresne
Organization: University of Manitoba
Title-Subject: [Filtered] Hitachi TM 1000
Message: Hi Everyone, The TM 1000 has been around for a while now and we have had our Turbo Molecular Pump (TMP) refurbished and replaced it ourselves in the past. We now have to repeat the process however the availability locate a contractor that will refurbish our non functioning pump is difficult to locate. If any TM 1000 user out there that has solved this riddle please contact me with a solution.
Merci.
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Today I observed some biological samples, prepared normally with the usual protocol. However, when I returned to the Auriga/Zeiss microscope after few hours, the 30 um aperture (the one that I worked at), stopped having image. I tried to align again, but does not work. I just see noise. The other apertures are working fine.
Has anyone experienced this situation?
I do not know what may have happened.
Best regards.
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Hello Daniela, when this happens to me (using old JEOL SEMS) it is likely that the beam axis has shifted. 1. you see noise = the PMT works fine 2. you see a picture using other apertures (I assume they have larger diameters)= the beam is nearly ok and the PMT works well.
conclusion: a) either the beam is shifted a few µm, or the aperture foil has moved, or the GUN shift and tilt controls on the control board has been altered. b) The aperture could also have been blocked or degraded by the beam. solution: a) adjust the GUN shift and tilt for maximum signal or adjust the µm knobs on the aperture holder. b) change aperture foil or clean it. Best regards Erik Ordell
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From pharmacy-best10-at-windelsmarx.com Fri Mar 21 02:18:48 2014 Return-Path: {pharmacy-best10-at-windelsmarx.com} Received: from mail4.windelsmarx.com (16.72.8.194.svyaz-nt.ru [194.8.72.16] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2L7IkHJ000756 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 21 Mar 2014 02:18:46 -0500 Message-Id: {201403210718.s2L7IkHJ000756-at-ns.microscopy.com}
I've been microtoming low Tg polymer recently and my cryo-microtome has the options of having the sample and knife at different temperatures.
How low? Well I'm working at -100C with a dry diamond knife and picking up the thin, crinkled sections with sugar water. I am not happy with the thin sections and I'm wonder if there is any advantage in having the knife and sample at different temperatures. It's an RCM Powertome with cryo attachment.
Any thoughts and experience I can draw on?
Thanks Frank Karl
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} When this happens to me (using old JEOL SEMS) it is likely that the beam axis has shifted.
This sounds like the case. The gun alignment is so far out that you can no longer get an image.
The Zeiss Auriga, working on the assumption that it's a Zeiss Gemini column, uses an intermediate lens to change apertures, so all of the adjustments are electronic. The first thing to check is the emission image on the 30um aperture. If your software is anything like mine, it will be be a button labelled "Emission" under the apertures tab. This will start the system rastering the gun alignment coils across the fixed aperture plate. On my system the scanning has to be set to speed 9, and then the "Gun Align" controls on the aperture tab can be used to adjust the gun such that the selected aperture is in the centre of the image.
In the emission image, you should be able to see all of the available apertures laid out in a circle. If the 30um aperture is not visible at all in the emission image (probably the centre aperture, depending on configuration) then you have bigger problems and a service call may be required. From what I understand, the gun has to be removed to access the aperture plate for replacement or cleaning.
If you don't have access to the gun alignment, or if it is so far out as to be uncorrectable, you'll have to log in to the system with a service level account to align it further. The user level accounts on Zeiss seem to apply a limited offset to the major alignment performed by the service engineers.
-Jacob
On 14-03-20 10:54 PM, erikordell-at-icloud.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Daniela, } when this happens to me (using old JEOL SEMS) it is likely that the beam axis has shifted. } 1. you see noise = the PMT works fine } 2. you see a picture using other apertures (I assume they have larger diameters)= the beam is nearly ok and the PMT works well. } } conclusion: a) either the beam is shifted a few µm, or the aperture foil has moved, or the GUN shift and tilt controls on the control board has been altered. b) The aperture could also have been blocked or degraded by the beam. } solution: a) adjust the GUN shift and tilt for maximum signal or adjust the µm knobs on the aperture holder. b) change aperture foil or clean it. } Best regards } Erik Ordell } } ==============================Original Headers============================== } 2, 37 -- From erikordell-at-icloud.com Fri Mar 21 00:46:52 2014 } 2, 37 -- Received: from st11p06mm-asmtp002.mac.com (st11p06mm-asmtp002.mac.com [17.172.124.250]) } 2, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2L5kqiY013716 } 2, 37 -- for {Microscopy-at-Microscopy.com} ; Fri, 21 Mar 2014 00:46:52 -0500 } 2, 37 -- MIME-version: 1.0 } 2, 37 -- Content-type: text/plain; charset=iso-8859-1 } 2, 37 -- Received: from [172.20.10.9] } 2, 37 -- (host-95-195-215-6.mobileonline.telia.com [95.195.215.6]) } 2, 37 -- by st11p06mm-asmtp002.mac.com } 2, 37 -- (Oracle Communications Messaging Server 7u4-27.08(7.0.4.27.7) 64bit (built Aug } 2, 37 -- 22 2013)) with ESMTPSA id {0N2R000WFUQ03N30-at-st11p06mm-asmtp002.mac.com} for } 2, 37 -- Microscopy-at-Microscopy.com; Thu, 20 Mar 2014 22:46:52 -0700 (PDT) } 2, 37 -- From: Erik Ordell {erikordell-at-icloud.com} } 2, 37 -- Date: Fri, 21 Mar 2014 06:46:45 +0100 } 2, 37 -- Subject: SEM aperture } 2, 37 -- To: Microscopy-at-Microscopy.com } 2, 37 -- Message-id: {0B102F7E-3A56-4D4C-B149-97D6C0E783F6-at-icloud.com} } 2, 37 -- X-Mailer: Apple Mail (2.1874) } 2, 37 -- x-icloud-spam-score: 34444444 } 2, 37 -- f=icloud.com;e=icloud.com;is=no;ir=yes;pp=ham;spf=n/a;dkim=n/a;dmarc=n/a;wl=n/a;pwl=n/a;clxs=n/a;clxl=n/a } 2, 37 -- X-MANTSH: 1TEIXWV4bG1oaGkdHB0lGUkdDRl5PWBoaHBEKTEMXGx0EGx0YBBIZBBscEBseGh8 } 2, 37 -- aEQpYTRdLEQptfhcaEQpMWRcbGhsbEQpZSRcRClleF2hjeREKQ04XSxsbGmJCH2hfHkB9GXhzB } 2, 37 -- xhmGh8eHFoZEQpYXBcZBBoEHQdNSx0SSEkcTAUbHQQbHRgEEhkEGxwQGx4aHxsRCl5ZF2Fzckh } 2, 37 -- HEQpDWhcTHwQbEx8EGBsfBBwRCkRYFxgRCkJFF21rfmxLbE5wf2BLEQpCThdrRRpSUB5DXFlca } 2, 37 -- BEKQkwXYh5SWVJGaWhEYgERCkJsF2tvYH9bGF9aH09iEQpCQBdsfF1DG35rcxsTexEKcGgXYkl } 2, 37 -- kYVtQUlwbHmkRCnBoF29MU1saaVMTXB9fEQpwaBdhAR0BHwVpWmV6QBEKcGgXZVpGYxxMExteT } 2, 37 -- UERCnBoF2ABWR9YcEwYex19EQpwbBd6aH1BZF9MckRSYRE= } 2, 37 -- X-CLX-Spam: false } 2, 37 -- X-CLX-Score: 1011 } 2, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure } 2, 37 -- engine=2.50.10432:5.11.87,1.0.14,0.0.0000 } 2, 37 -- definitions=2014-03-21_02:2014-03-20,2014-03-21,1970-01-01 signatures=0 } 2, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 } 2, 37 -- suspectscore=1 phishscore=0 adultscore=0 bulkscore=0 classifier=spam adjust=0 } 2, 37 -- reason=mlx scancount=1 engine=7.0.1-1402240000 definitions=main-1403200191 } 2, 37 -- Content-Transfer-Encoding: 8bit } 2, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2L5kqiY013716 } ==============================End of - Headers============================== } }
-- Jacob Kabel Electron Microscopist Department of Materials Engineering The University of British Columbia 6350 Stores Road, Room 419 Vancouver, British Columbia Canada V6T 1Z4 Phone: (604) 822-5648 Fax: (604) 822-3619 http://emlab.mtrl.ubc.ca/
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Message: hi ,can i study amber under the scanning electron microscope in high vacuum mode.
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Good Day: Â We are doing high pressure freezing and freeze substitution on plant, animal and bacterial samples. We are using Epon resin for embedding but struggle with its viscosity. Our experience with Spurr's has been disappointing in that we get no or reverse contrast in the TEM. We love the ease of use but can't make it work for imaging. Â Â We are using recipes from Glauert (firm and the kit) and have both a new kit and older stocks of the original ingredients. It doesn't seem to make a difference. Â Â Â Does anyone have any advice on how we can make and use this resin to produce samples that produce publication quality contrast in our images? Thanks bob
-- Electron Microscopy Unit New Science Complex 50 Stone Rd E University of Guelph Guelph, Ontario, Canada, N1G 2W1 Phone:  519-824-4120  ext.  56409 Fax  :  519-837-1802
==============================Original Headers============================== 3, 24 -- From bharris-at-uoguelph.ca Mon Mar 24 07:47:58 2014 3, 24 -- Received: from esa-jnhn.mail.uoguelph.ca (esa-jnhn.mail.uoguelph.ca [131.104.91.44]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2OClwtO018824 3, 24 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 07:47:58 -0500 3, 24 -- X-IronPort-Anti-Spam-Filtered: true 3, 24 -- X-IronPort-Anti-Spam-Result: AqEEAMsoMFODaFvc/2dsb2JhbABWA4QYgwe8XYMOgSx0gk9DIScCDRkCHUKIDJxWjxuiERcEgSWNHSQogl6BSQSqe4NJIYFu 3, 24 -- X-IronPort-AV: E=Sophos;i="4.97,720,1389762000"; 3, 24 -- d="scan'208";a="108586027" 3, 24 -- Received: from nipigon.cs.uoguelph.ca (HELO zcs1.mail.uoguelph.ca) ([131.104.91.220]) 3, 24 -- by esa-jnhn.mail.uoguelph.ca with ESMTP; 24 Mar 2014 08:46:38 -0400 3, 24 -- Received: from zcs1.mail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 3, 24 -- by zcs1.mail.uoguelph.ca (Postfix) with ESMTP id 2B43F6AE72 3, 24 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 08:46:38 -0400 (EDT) 3, 24 -- Date: Mon, 24 Mar 2014 08:46:38 -0400 (EDT) 3, 24 -- From: Robert J Harris {bharris-at-uoguelph.ca} 3, 24 -- To: "microscopy-at-microscopy.com microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 24 -- Message-ID: {1913685812.868484.1395665198155.JavaMail.root-at-uoguelph.ca} 3, 24 -- Subject: Spurr's Resin 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; charset=utf-8 3, 24 -- X-Originating-IP: [172.17.91.203] 3, 24 -- X-Mailer: Zimbra 7.2.1_GA_2790 (ZimbraWebClient - SAF3 (Mac)/7.2.1_GA_2790) 3, 24 -- Content-Transfer-Encoding: 8bit 3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2OClwtO018824 ==============================End of - Headers==============================
Interesting question... I suspect charging will be a major problem, so I would suggest a gold coating and a little extra effort to make sure the sample is grounded. I've worked with rubber samples from belts, tires and gaskets in my SEM without any significant problems, despite the amount of volatile components present.
But these SEM were all oil pumped and a thin layer of oil was always present on the EDS detector and everywhere else too. I suspect amber might give you some volatility problems, so I'd consider using the smallest piece possible and if you had a low pressure SEM, I'd jump on that.
Too bad you not a member of the listserver, it's free and you could tell use how it works out for you.
Frank Karl
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Monday, March 24, 2014 8:40 AM To: Frank Karl
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Message: hi ,can i study amber under the scanning electron microscope in high vacuum mode.
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==============================Original Headers============================== 24, 27 -- From frank_karl-at-ardl.com Mon Mar 24 07:57:37 2014 24, 27 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 24, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2OCvano005569 24, 27 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 07:57:36 -0500 24, 27 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 24, 27 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Mon, 24 24, 27 -- Mar 2014 08:57:35 -0400 24, 27 -- From: Frank Karl {frank_karl-at-ardl.com} 24, 27 -- To: "'hadeelzh-at-yahoo.com'" {hadeelzh-at-yahoo.com} 24, 27 -- CC: "Microscopy Listserver (microscopy-at-microscopy.com)" 24, 27 -- {microscopy-at-microscopy.com} 24, 27 -- Date: Mon, 24 Mar 2014 08:57:35 -0400 24, 27 -- Subject: RE: [Microscopy] viaWWW:amber on sem 24, 27 -- Thread-Topic: [Microscopy] viaWWW:amber on sem 24, 27 -- Thread-Index: Ac9HXkRGF+kZvuufTM24SK00ykMnjQAAQnwg 24, 27 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BE5270922-at-exchange2k7.ad.ardl.com} 24, 27 -- References: {201403241240.s2OCe054013330-at-ns.microscopy.com} 24, 27 -- In-Reply-To: {201403241240.s2OCe054013330-at-ns.microscopy.com} 24, 27 -- Accept-Language: en-US 24, 27 -- Content-Language: en-US 24, 27 -- X-MS-Has-Attach: 24, 27 -- X-MS-TNEF-Correlator: 24, 27 -- acceptlanguage: en-US 24, 27 -- Content-Type: text/plain; charset="us-ascii" 24, 27 -- MIME-Version: 1.0 24, 27 -- Content-Transfer-Encoding: 8bit 24, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2OCvano005569 ==============================End of - Headers==============================
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Hello Bob, I agree, Spurr's resin is great for viscosity but it's contrast is poor. Also, I've never liked cutting Spurr's. In my hands it is brittle and difficult to face and trim. As a remedy, I now use a Spurr's / Epon combination. It give great contrast and is MUCH easier to cut. Let me know if you would like my recipe and I can forward it to you. Take care, Pat Kysar, C.E.M.T. Chair, Certification Board, Microscopy Society of America University of California, Davis School of Medicine Dept of Pathology and Laboratory Medicine Electron Microscopy Lab
-----Original Message----- X-from: bharris-at-uoguelph.ca [mailto:bharris-at-uoguelph.ca] Sent: Monday, March 24, 2014 5:57 AM To: pekysar-at-ucdavis.edu
Good Day: Â We are doing high pressure freezing and freeze substitution on plant, animal and bacterial samples. We are using Epon resin for embedding but struggle with its viscosity. Our experience with Spurr's has been disappointing in that we get no or reverse contrast in the TEM. We love the ease of use but can't make it work for imaging. Â Â We are using recipes from Glauert (firm and the kit) and have both a new kit and older stocks of the original ingredients. It doesn't seem to make a difference. Â Â Â Does anyone have any advice on how we can make and use this resin to produce samples that produce publication quality contrast in our images? Thanks bob
-- Electron Microscopy Unit New Science Complex 50 Stone Rd E University of Guelph Guelph, Ontario, Canada, N1G 2W1 Phone:  519-824-4120  ext.  56409 Fax  :  519-837-1802
==============================Original Headers============================== 3, 24 -- From bharris-at-uoguelph.ca Mon Mar 24 07:47:58 2014 3, 24 -- Received: from esa-jnhn.mail.uoguelph.ca (esa-jnhn.mail.uoguelph.ca [131.104.91.44]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2OClwtO018824 3, 24 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 07:47:58 -0500 3, 24 -- X-IronPort-Anti-Spam-Filtered: true 3, 24 -- X-IronPort-Anti-Spam-Result: AqEEAMsoMFODaFvc/2dsb2JhbABWA4QYgwe8XYMOgSx0gk9DIScCDRkCHUKIDJxWjxuiERcEgSWN HSQogl6BSQSqe4NJIYFu 3, 24 -- X-IronPort-AV: E=Sophos;i="4.97,720,1389762000"; 3, 24 -- d="scan'208";a="108586027" 3, 24 -- Received: from nipigon.cs.uoguelph.ca (HELO zcs1.mail.uoguelph.ca) ([131.104.91.220]) 3, 24 -- by esa-jnhn.mail.uoguelph.ca with ESMTP; 24 Mar 2014 08:46:38 -0400 3, 24 -- Received: from zcs1.mail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 3, 24 -- by zcs1.mail.uoguelph.ca (Postfix) with ESMTP id 2B43F6AE72 3, 24 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 08:46:38 -0400 (EDT) 3, 24 -- Date: Mon, 24 Mar 2014 08:46:38 -0400 (EDT) 3, 24 -- From: Robert J Harris {bharris-at-uoguelph.ca} 3, 24 -- To: "microscopy-at-microscopy.com microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 24 -- Message-ID: {1913685812.868484.1395665198155.JavaMail.root-at-uoguelph.ca} 3, 24 -- Subject: Spurr's Resin 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; charset=utf-8 3, 24 -- X-Originating-IP: [172.17.91.203] 3, 24 -- X-Mailer: Zimbra 7.2.1_GA_2790 (ZimbraWebClient - SAF3 (Mac)/7.2.1_GA_2790) 3, 24 -- Content-Transfer-Encoding: 8bit 3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2OClwtO018824 ==============================End of - Headers==============================
==============================Original Headers============================== 11, 28 -- From pekysar-at-ucdavis.edu Mon Mar 24 09:55:51 2014 11, 28 -- Received: from msa1.ucdavis.edu (msa1.ucdavis.edu [128.120.32.181]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2OEtoaK026078 11, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 09:55:50 -0500 11, 28 -- Received: from MPAT6723 ([169.237.197.188]) 11, 28 -- (authenticated bits=0) 11, 28 -- by msa1.ucdavis.edu (8.14.4/8.14.5/it-rhel5-mimedefang-smtp-1.6) with ESMTP id s2OEtko8039184 11, 28 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 11, 28 -- Mon, 24 Mar 2014 07:55:47 -0700 11, 28 -- From: "Patricia Kysar" {pekysar-at-ucdavis.edu} 11, 28 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 11, 28 -- Cc: {bharris-at-uoguelph.ca} 11, 28 -- References: {201403241256.s2OCuVMK004833-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201403241256.s2OCuVMK004833-at-ns.microscopy.com} 11, 28 -- Subject: RE: [Microscopy] Spurr's Resin 11, 28 -- Date: Mon, 24 Mar 2014 07:55:46 -0700 11, 28 -- Message-ID: {002b01cf4771$23af20b0$6b0d6210$-at-ucdavis.edu} 11, 28 -- MIME-Version: 1.0 11, 28 -- Content-Type: text/plain; 11, 28 -- charset="iso-8859-1" 11, 28 -- X-Mailer: Microsoft Outlook 14.0 11, 28 -- Thread-Index: AQGma0ZKMcRr3XgkIKyDSZ7oxhhca5tCCpBg 11, 28 -- Content-Language: en-us 11, 28 -- X-Virus-Scanned: clamav-milter 0.98.1 at msa1 11, 28 -- X-Virus-Status: Clean 11, 28 -- X-Scanned-By: MIMEDefang 2.74 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2OEtoaK026078 ==============================End of - Headers==============================
I am in the process of trying to get an used JSM-5400 for my EM lab near Lisbon (non profit). However the old (but functioning) microsope does not have the BS detector (we need the best BSE images we can get) and the old NORAN X-EDS attached to the microscope system does now work and can not be recovered. We are also lacking funding to get new detectors, so I would like to ask if any of you have unused detectors that can be offered or obtained at a low cost. We will take full care of the transport of course. Commercial refurbished systems are also an option if not too expensive. In case you wish to contact me directelly please use the e-mail apamatos-at-kanguru.pt or apamatos-at-microventures.co
Many thanks A.P. Alves de Matos Biologist Ph.D. Egas Moniz Electron Microscopy Unit, Caparica, Portugal
==============================Original Headers============================== 3, 33 -- From apamatos-at-gmail.com Mon Mar 24 10:24:16 2014 3, 33 -- Received: from mail-we0-f179.google.com (mail-we0-f179.google.com [74.125.82.179]) 3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2OFOGng013266 3, 33 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 10:24:16 -0500 3, 33 -- Received: by mail-we0-f179.google.com with SMTP id x48so3421942wes.24 3, 33 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 08:24:15 -0700 (PDT) 3, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 33 -- d=gmail.com; s=20120113; 3, 33 -- h=message-id:date:from:user-agent:mime-version:to:subject 3, 33 -- :content-type:content-transfer-encoding; 3, 33 -- bh=RrEpxeo7y8faeKwW2JWCD5I6cpmvoMLzlP8jlNL0G9M=; 3, 33 -- b=PpZIX5X+t86IFosmOsDVyg+G4pfMJh2n830DBjNXMDs9iGrWx6XIDztnYnScvGgUTn 3, 33 -- lET/vpnOB7PAcZv3SbgjGgAKnFPP54tG+Qg6UmRqNTIi8Aj6JP7s3eyn+ipdJ/J42n3d 3, 33 -- 37yssBbr9nOkWepXJqrPceLTi6V9JTsDOpSIO1KUc6S1wY9lp3BLLzcp2HRt9P48YCCq 3, 33 -- TlWjTFai0RfvdhoyRYfXI4i5m64SFoBOMO60WgIdN33Jhrkt1OaplMgCT4l0aRzH8r0A 3, 33 -- aJeN7HhEIMTTj2R/Sz7GMDcjTyol0VedUvtq+mIKphRqT1GsyWoG+32GvZ/bJz3HElYM 3, 33 -- T2xg== 3, 33 -- X-Received: by 10.180.109.231 with SMTP id hv7mr16828958wib.38.1395674655496; 3, 33 -- Mon, 24 Mar 2014 08:24:15 -0700 (PDT) 3, 33 -- Received: from localhost.localdomain ([87.196.131.189]) 3, 33 -- by mx.google.com with ESMTPSA id n15sm39538404wij.3.2014.03.24.08.24.12 3, 33 -- for {microscopy-at-microscopy.com} 3, 33 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 3, 33 -- Mon, 24 Mar 2014 08:24:13 -0700 (PDT) 3, 33 -- Message-ID: {53304E1B.7000108-at-gmail.com} 3, 33 -- Date: Mon, 24 Mar 2014 15:24:11 +0000 3, 33 -- From: AP Alves de Matos {apamatos-at-gmail.com} 3, 33 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 3, 33 -- MIME-Version: 1.0 3, 33 -- To: microscopy-at-microscopy.com 3, 33 -- Subject: TEM Need backscattered and/or X-EDS detectors for an used JSM-5400 3, 33 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 33 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Do you know where I can find a K250 Carbon Coating Attachment for an Emitech K550 sputter coater?
Thank you!
Valerie Lecomte
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Mar 24 17:05:01 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2OM51AJ007526 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 17:05:01 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 484486DAB84 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 17:05:01 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id YPBLhE8lfLRt for {microscopy-at-microscopy.com} ; 16, 28 -- Mon, 24 Mar 2014 17:04:19 -0500 (CDT) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 80FD66DAB76 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2014 17:04:19 -0500 (CDT) 16, 28 -- Message-ID: {5330ABE3.2070007-at-microscopy.com} 16, 28 -- Date: Mon, 24 Mar 2014 17:04:19 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.7; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:K250 carbon coating attachment 16, 28 -- References: {201403241636.s2OGaskk001790-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201403241636.s2OGaskk001790-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201403241636.s2OGaskk001790-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From reasonable-meds12-at-idea-tele.com Tue Mar 25 01:41:54 2014 Return-Path: {reasonable-meds12-at-idea-tele.com} Received: from c-254-62.idea-tele.com (c-254-62.idea-tele.com [77.72.254.62] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2P6fpWd030542 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 25 Mar 2014 01:41:52 -0500 Message-Id: {201403250641.s2P6fpWd030542-at-ns.microscopy.com}
Dear colleagues, i have a question regarding the analysis of statistics, I apologize if this is not directly related to microscopy but I know that some of you are hardcore statisticians and I already got help in the past. If I compare the effect of product A and B (whatever effect it doesn't matter) and I find with the Student-t test that p (A)=0,04 and p (B)=0,005, may I say that B works better than A? I always thought that this interpretation of statistics is not allowed but I now see it in a recent Science paper and I surprised. Many thanks in advance. Stephane
==============================Original Headers============================== 2, 38 -- From nizets2-at-yahoo.com Tue Mar 25 04:54:11 2014 2, 38 -- Received: from nm10-vm0.bullet.mail.bf1.yahoo.com (nm10-vm0.bullet.mail.bf1.yahoo.com [98.139.213.147]) 2, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2P9sBLj001905 2, 38 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2014 04:54:11 -0500 2, 38 -- Received: from [98.139.215.142] by nm10.bullet.mail.bf1.yahoo.com with NNFMP; 25 Mar 2014 09:54:10 -0000 2, 38 -- Received: from [98.139.212.212] by tm13.bullet.mail.bf1.yahoo.com with NNFMP; 25 Mar 2014 09:54:10 -0000 2, 38 -- Received: from [127.0.0.1] by omp1021.mail.bf1.yahoo.com with NNFMP; 25 Mar 2014 09:54:10 -0000 2, 38 -- X-Yahoo-Newman-Property: ymail-3 2, 38 -- X-Yahoo-Newman-Id: 963769.30680.bm-at-omp1021.mail.bf1.yahoo.com 2, 38 -- Received: (qmail 96964 invoked by uid 60001); 25 Mar 2014 09:54:10 -0000 2, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1395741250; bh=TgAh1AUIMRqLtB17VFETQ5op2k528NeYoAzrDfQB1ok=; h=X-YMail-OSG:Received:X-Rocket-MIMEInfo:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type; b=HkjNuwYsFdzAjckEBJ/lmoxObuQAfsciVFnCFF/qv6mArVhHw2YTtXT4IC2fmjJtENl2XsGWQtMxq4RpW7LIeSZPEFUs5ady/n2aLFVSigBnZVyFDQwuIql37jz+QxwquPjXHiWZFVDSqmJ+O19mRF9QcHCwlXUz3WmAACmGXtI= 2, 38 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 2, 38 -- s=s1024; d=yahoo.com; 2, 38 -- h=X-YMail-OSG:Received:X-Rocket-MIMEInfo:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type; 2, 38 -- b=R3XRDndiJpBFY6pEVg+Uc1Y/UVwk7YMwmFlBXu5MCqId+UcpWpa5FKne9Gbzmi0XGuaSgjUPkUDEKonFwlNuwn+1UbVVmQ5oKFFICv2xtddtNnKo2/9JXB63xX1wHaGXxaHn82E4RSDdvtuJix27ByMTrQMAIbFvm0bkVc1UUVc=; 2, 38 -- X-YMail-OSG: oj2oRSMVM1k4LJYH9IK4zHbbR8xhhMY6xT4X.w80xHXKXrk 2, 38 -- T44011EY9T8txO32TTbcbRiUOJpnURNuGfRDJUwnXS58EhPgupk94srVf.AM 2, 38 -- AB_iRJNIKnhWkRttxdXQsgDR0l7_nbvWJh9JVteeZV0K9uOQEWD4tvkvbrjH 2, 38 -- qeUzYsgSIOYBK9JfdOubTvujoZKMrjx6wRnPs7NoSeiUPhUgimqO.mJRj883 2, 38 -- VCyVnvKUni6fkkiFhBIvpBOx1wCTy7xzTtnYAdrcuNNtx7yzZwQlyPl5YyRx 2, 38 -- eGehVEXEDOYsIK7j7qF0_1O9PzFKq8iaGecCxL5pEd6zQeik1rAkLL5riFQd 2, 38 -- YlJwyoUZh2GnG3uxhpKKj5Q1tybe0Wn7zemLpmODp8CeMtWvd_D7g8UPSmY0 2, 38 -- 655QC8JTRtTtFGSX_LYR0_T9v.peD_o0EZFh6MEsFT57BrCfXvirU_bnZRFo 2, 38 -- tYEVxEeb7WPieVRkOBdcrs4FSRr0xupxnl8yHYaxVvQ87waNkwqycwm4tdsq 2, 38 -- JRWAWs9OkBa332cwpVQcvd_XhH665BIlUZVtAdKF0tJFhaSQv 2, 38 -- Received: from [80.122.101.98] by web141704.mail.bf1.yahoo.com via HTTP; Tue, 25 Mar 2014 02:54:10 PDT 2, 38 -- X-Rocket-MIMEInfo: 002.001,CkRlYXIgY29sbGVhZ3VlcywKaSBoYXZlIGEgcXVlc3Rpb24gcmVnYXJkaW5nIHRoZSBhbmFseXNpcyBvZiBzdGF0aXN0aWNzLCBJIGFwb2xvZ2l6ZSBpZiB0aGlzIGlzIG5vdCBkaXJlY3RseSByZWxhdGVkIHRvIG1pY3Jvc2NvcHkgYnV0IEkga25vdyB0aGF0IHNvbWUgb2YgeW91IGFyZSBoYXJkY29yZSBzdGF0aXN0aWNpYW5zIGFuZCBJIGFscmVhZHkgZ290IGhlbHAgaW4gdGhlIHBhc3QuCklmIEkgY29tcGFyZSB0aGUgZWZmZWN0IG9mIHByb2R1Y3QgQSBhbmQgQiAod2hhdGV2ZXIgZWZmZWN0IGl0IGRvZXMBMAEBAQE- 2, 38 -- X-Mailer: YahooMailWebService/0.8.181.645 2, 38 -- References: {201403250949.s2P9nFj5001824-at-ns.microscopy.com} 2, 38 -- Message-ID: {1395741250.94119.YahooMailNeo-at-web141704.mail.bf1.yahoo.com} 2, 38 -- Date: Tue, 25 Mar 2014 02:54:10 -0700 (PDT) 2, 38 -- From: Stephane Nizet {nizets2-at-yahoo.com} 2, 38 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 2, 38 -- Subject: Fw: overinterpretation of statistics 2, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 38 -- In-Reply-To: {201403250949.s2P9nFj5001824-at-ns.microscopy.com} 2, 38 -- MIME-Version: 1.0 2, 38 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
From auosueoa-at-yota.ru Tue Mar 25 06:41:23 2014 Return-Path: {auosueoa-at-yota.ru} Received: from client.yota.ru (client.yota.ru [188.162.37.17] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2PBfL8J022953 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 25 Mar 2014 06:41:22 -0500 Message-Id: {201403251141.s2PBfL8J022953-at-ns.microscopy.com}
Stephane, At the risk of making a total fool of myself, I'll jump in with the disclaimer that I am not a statistician.
But from what I understand, the first problem with what you wrote is that p values represent a probalistic argument and are not equalities. So should be p { x, never p = x. (This probably doesn't matter to your story but just mentioning it)
Next, a t-test is typically done to tell whether two means are equal. So in your case I assume that there were two experiments, one for A and the other for B but in each case there was a control treatment, "C". Thus, the first test (A vs C) shows that there is no evidence to reject the hypothesis that the means are equal. The reported difference would happen by chance more than once in twenty times, which is generally considered not good enough to be "evidence". Though it can be taken as a 'trend'. But the next test (B vs C) shows that the equality of means can be rejected because the experimentally observed difference should happen by chance less than once per hundred times.
So from this, there is no evidence that A works (ie, differs from control). But there is evidence that B does. Therefore seems only a logician (smile) would object to claiming that B works better than A.
Anyway that is my take from the trenches. Maybe some professionals will weigh in?
As ever, Tobias
On 3/25/14, 5:54 AM, nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear colleagues, } i have a question regarding the analysis of statistics, I apologize if this is not directly related to microscopy but I know that some of you are hardcore statisticians and I already got help in the past. } If I compare the effect of product A and B (whatever effect it doesn't matter) and I find with the Student-t test that p (A)=0,04 and p (B)=0,005, may I say that B works better than A? } I always thought that this interpretation of statistics is not allowed but I now see it in a recent Science paper and I surprised. } Many thanks in advance. } Stephane }
-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 USA / /___ / \ \___/ \_____ 413 - 545 - 1533 www.bio.umass.edu/biology/baskin
==============================Original Headers============================== 10, 22 -- From baskin-at-bio.umass.edu Tue Mar 25 07:39:40 2014 10, 22 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2PCdeE1023686 10, 22 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2014 07:39:40 -0500 10, 22 -- Received: from rabbitbyte.local (ip-159.250.65.141.dsl.crocker.net [159.250.65.141]) 10, 22 -- (authenticated bits=0) 10, 22 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id s2PCdcBT003659 10, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO); 10, 22 -- Tue, 25 Mar 2014 08:39:39 -0400 (EDT) 10, 22 -- Message-ID: {5331790B.2060201-at-bio.umass.edu} 10, 22 -- Date: Tue, 25 Mar 2014 08:39:39 -0400 10, 22 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 10, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 10, 22 -- MIME-Version: 1.0 10, 22 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com 10, 22 -- Subject: Re: [Microscopy] Fw: overinterpretation of statistics 10, 22 -- References: {201403250954.s2P9sm9p002513-at-ns.microscopy.com} 10, 22 -- In-Reply-To: {201403250954.s2P9sm9p002513-at-ns.microscopy.com} 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 22 -- Content-Transfer-Encoding: 7bit 10, 22 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Tue, 25 Mar 2014 08:39:39 -0400 (EDT) 10, 22 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
Also not a statistician by trade, nor do I play one on TV. I do use them, though. A p value result in a statistical test tells you the probability of obtaining a result. Typically one sets an alpha value at 0.05 as a limit to exceed where you accept or reject the null hypothesis. Or, to simplify, in the case of a t-test for 2 means, your null hypothesis is "the 2 means are the same", and you set an alpha error limit at 1 out of 20, or 0.05. You perform the test and get a p value of 0.03, which is below alpha (YAY!), and report it as p {0.05 and conclude you can reject the null hypothesis and decide the 2 means are different. Statistically you are saying that if you did the experiment 20 times, you would expect this outcome 19 times. The p value tells you nothing about the magnitude of the difference between the means. It only tells you that one test is even more (or less) likely to have rejected the null hypothesis of "no difference". So, yes, the situation as you (Stephane) described would be an "over interpretation". You should be surprised to see such a conclusion in a Science paper. I would be shocked.
If you want to compare the magnitude of difference of two conditions (say, A and B) from a control (C), you would choose to run a statistical test built to tell you that, like a simple one-way analysis of variance (ANOVA). There are post-hoc tests to the ANOVA which allow you to compare the magnitude of the A to C and B to C differences, with yet another p value to report how confident you are about the size of that difference.
This brings me to mention a pet peeve, which is the over-use of Student's t-test in probing a set of data. When a t-test is run multiple times on the same set (or subset) of data, the alpha value (or probability of committing a Type I error of accepting a false positive) increases. This means that if you plan to do that, you need to set a lower alpha value, like 0.01, in order to find reliable effects. This is why more complex statistics like ANOVA are helpful ... you can still use 0.05 for alpha while simultaneously looking for differences between multiple combinations of data in a single test, then probe for trends and magnitudes with post-hoc tools. Just my $0.02 ...
Thomas Trusk, PhD Director, Josh Spruill Imaging Facility Dept of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston, SC 29425
-----Original Message-----
Stephane, At the risk of making a total fool of myself, I'll jump in with the disclaimer that I am not a statistician.
But from what I understand, the first problem with what you wrote is that p values represent a probalistic argument and are not equalities. So should be p { x, never p = x. (This probably doesn't matter to your story but just mentioning it)
Next, a t-test is typically done to tell whether two means are equal. So in your case I assume that there were two experiments, one for A and the other for B but in each case there was a control treatment, "C". Thus, the first test (A vs C) shows that there is no evidence to reject the hypothesis that the means are equal. The reported difference would happen by chance more than once in twenty times, which is generally considered not good enough to be "evidence". Though it can be taken as a 'trend'. But the next test (B vs C) shows that the equality of means can be rejected because the experimentally observed difference should happen by chance less than once per hundred times.
So from this, there is no evidence that A works (ie, differs from control). But there is evidence that B does. Therefore seems only a logician (smile) would object to claiming that B works better than A.
Anyway that is my take from the trenches. Maybe some professionals will weigh in?
As ever, Tobias
} } } Dear colleagues, } i have a question regarding the analysis of statistics, I apologize if this is not directly related to microscopy but I know that some of you are hardcore statisticians and I already got help in the past. } If I compare the effect of product A and B (whatever effect it doesn't matter) and I find with the Student-t test that p (A)=0,04 and p (B)=0,005, may I say that B works better than A? } I always thought that this interpretation of statistics is not allowed but I now see it in a recent Science paper and I surprised. } Many thanks in advance. } Stephane }
-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 USA / /___ / \ \___/ \_____ 413 - 545 - 1533 www.bio.umass.edu/biology/baskin
==============================Original Headers============================== 16, 31 -- From truskt-at-musc.edu Tue Mar 25 11:14:37 2014 16, 31 -- Received: from ironp3.musc.edu (ironp3.musc.edu [128.23.34.116]) 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2PGEX9O013236 16, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Mar 2014 11:14:35 -0500 16, 31 -- X-IronPort-GLBA: NPI-Med 16, 31 -- X-IronPort-AV: E=Sophos;i="4.97,728,1389762000"; 16, 31 -- d="scan'208";a="156520865" 16, 31 -- Received: from mailhost.musc.edu (exg-hub11a.mdc.musc.edu [128.23.180.169]) 16, 31 -- by revere5a.musc.edu (8.13.5/8.13.5) with ESMTP id s2PG7vAX006348 16, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Mar 2014 12:07:57 -0400 16, 31 -- Received: from EXG-MB11A.clinlan.local ([169.254.3.88]) by 16, 31 -- exg-hub11a.clinlan.local ([fe80::dc7a:e2b:6a4e:19ae%13]) with mapi id 16, 31 -- 14.03.0174.001; Tue, 25 Mar 2014 12:07:15 -0400 16, 31 -- From: "Trusk, Thomas C." {truskt-at-musc.edu} 16, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 16, 31 -- Subject: RE: [Microscopy] Re: Fw: overinterpretation of statistics 16, 31 -- Thread-Topic: [Microscopy] Re: Fw: overinterpretation of statistics 16, 31 -- Thread-Index: AQHPSCii9pdnw9UsdEClVdspIz1unJrx7Eow 16, 31 -- Date: Tue, 25 Mar 2014 16:07:15 +0000 16, 31 -- Message-ID: {772DAC962C154B4E8D7F180232405D9F190A6C6C-at-exg-mb11a.clinlan.local} 16, 31 -- References: {201403251249.s2PCnA40002245-at-ns.microscopy.com} 16, 31 -- In-Reply-To: {201403251249.s2PCnA40002245-at-ns.microscopy.com} 16, 31 -- Accept-Language: en-US 16, 31 -- Content-Language: en-US 16, 31 -- X-MS-Has-Attach: 16, 31 -- X-MS-TNEF-Correlator: 16, 31 -- x-originating-ip: [128.23.180.200] 16, 31 -- Content-Type: text/plain; charset="us-ascii" 16, 31 -- MIME-Version: 1.0 16, 31 -- Content-Transfer-Encoding: 8bit 16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2PGEX9O013236 ==============================End of - Headers==============================
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And Students t-test, ANOVA & others also assume data points are normally distributed,another wrinkle...
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 ________________________________________ X-from: truskt-at-musc.edu [truskt-at-musc.edu] Sent: Wednesday, 26 March 2014 3:21 a.m. To: White, Rosemary (PI, Black Mountain)
Also not a statistician by trade, nor do I play one on TV. I do use them, though. A p value result in a statistical test tells you the probability of obtaining a result. Typically one sets an alpha value at 0.05 as a limit to exceed where you accept or reject the null hypothesis. Or, to simplify, in the case of a t-test for 2 means, your null hypothesis is "the 2 means are the same", and you set an alpha error limit at 1 out of 20, or 0.05. You perform the test and get a p value of 0.03, which is below alpha (YAY!), and report it as p {0.05 and conclude you can reject the null hypothesis and decide the 2 means are different. Statistically you are saying that if you did the experiment 20 times, you would expect this outcome 19 times. The p value tells you nothing about the magnitude of the difference between the means. It only tells you that one test is even more (or less) likely to have rejected the null hypothesis of "no difference". So, yes, the situation as you (S! tephane) described would be an "over interpretation". You should be surprised to see such a conclusion in a Science paper. I would be shocked.
If you want to compare the magnitude of difference of two conditions (say, A and B) from a control (C), you would choose to run a statistical test built to tell you that, like a simple one-way analysis of variance (ANOVA). There are post-hoc tests to the ANOVA which allow you to compare the magnitude of the A to C and B to C differences, with yet another p value to report how confident you are about the size of that difference.
This brings me to mention a pet peeve, which is the over-use of Student's t-test in probing a set of data. When a t-test is run multiple times on the same set (or subset) of data, the alpha value (or probability of committing a Type I error of accepting a false positive) increases. This means that if you plan to do that, you need to set a lower alpha value, like 0.01, in order to find reliable effects. This is why more complex statistics like ANOVA are helpful ... you can still use 0.05 for alpha while simultaneously looking for differences between multiple combinations of data in a single test, then probe for trends and magnitudes with post-hoc tools. Just my $0.02 ...
Thomas Trusk, PhD Director, Josh Spruill Imaging Facility Dept of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston, SC 29425
-----Original Message-----
Stephane, At the risk of making a total fool of myself, I'll jump in with the disclaimer that I am not a statistician.
But from what I understand, the first problem with what you wrote is that p values represent a probalistic argument and are not equalities. So should be p { x, never p = x. (This probably doesn't matter to your story but just mentioning it)
Next, a t-test is typically done to tell whether two means are equal. So in your case I assume that there were two experiments, one for A and the other for B but in each case there was a control treatment, "C". Thus, the first test (A vs C) shows that there is no evidence to reject the hypothesis that the means are equal. The reported difference would happen by chance more than once in twenty times, which is generally considered not good enough to be "evidence". Though it can be taken as a 'trend'. But the next test (B vs C) shows that the equality of means can be rejected because the experimentally observed difference should happen by chance less than once per hundred times.
So from this, there is no evidence that A works (ie, differs from control). But there is evidence that B does. Therefore seems only a logician (smile) would object to claiming that B works better than A.
Anyway that is my take from the trenches. Maybe some professionals will weigh in?
As ever, Tobias
} } } Dear colleagues, } i have a question regarding the analysis of statistics, I apologize if this is not directly related to microscopy but I know that some of you are hardcore statisticians and I already got help in the past. } If I compare the effect of product A and B (whatever effect it doesn't matter) and I find with the Student-t test that p (A)=0,04 and p (B)=0,005, may I say that B works better than A? } I always thought that this interpretation of statistics is not allowed but I now see it in a recent Science paper and I surprised. } Many thanks in advance. } Stephane }
-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 USA / /___ / \ \___/ \_____ 413 - 545 - 1533 www.bio.umass.edu/biology/baskin
==============================Original Headers============================== 16, 31 -- From truskt-at-musc.edu Tue Mar 25 11:14:37 2014 16, 31 -- Received: from ironp3.musc.edu (ironp3.musc.edu [128.23.34.116]) 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2PGEX9O013236 16, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Mar 2014 11:14:35 -0500 16, 31 -- X-IronPort-GLBA: NPI-Med 16, 31 -- X-IronPort-AV: E=Sophos;i="4.97,728,1389762000"; 16, 31 -- d="scan'208";a="156520865" 16, 31 -- Received: from mailhost.musc.edu (exg-hub11a.mdc.musc.edu [128.23.180.169]) 16, 31 -- by revere5a.musc.edu (8.13.5/8.13.5) with ESMTP id s2PG7vAX006348 16, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Mar 2014 12:07:57 -0400 16, 31 -- Received: from EXG-MB11A.clinlan.local ([169.254.3.88]) by 16, 31 -- exg-hub11a.clinlan.local ([fe80::dc7a:e2b:6a4e:19ae%13]) with mapi id 16, 31 -- 14.03.0174.001; Tue, 25 Mar 2014 12:07:15 -0400 16, 31 -- From: "Trusk, Thomas C." {truskt-at-musc.edu} 16, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 16, 31 -- Subject: RE: [Microscopy] Re: Fw: overinterpretation of statistics 16, 31 -- Thread-Topic: [Microscopy] Re: Fw: overinterpretation of statistics 16, 31 -- Thread-Index: AQHPSCii9pdnw9UsdEClVdspIz1unJrx7Eow 16, 31 -- Date: Tue, 25 Mar 2014 16:07:15 +0000 16, 31 -- Message-ID: {772DAC962C154B4E8D7F180232405D9F190A6C6C-at-exg-mb11a.clinlan.local} 16, 31 -- References: {201403251249.s2PCnA40002245-at-ns.microscopy.com} 16, 31 -- In-Reply-To: {201403251249.s2PCnA40002245-at-ns.microscopy.com} 16, 31 -- Accept-Language: en-US 16, 31 -- Content-Language: en-US 16, 31 -- X-MS-Has-Attach: 16, 31 -- X-MS-TNEF-Correlator: 16, 31 -- x-originating-ip: [128.23.180.200] 16, 31 -- Content-Type: text/plain; charset="us-ascii" 16, 31 -- MIME-Version: 1.0 16, 31 -- Content-Transfer-Encoding: 8bit 16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2PGEX9O013236 ==============================End of - Headers==============================
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Could someone please let me know if one can glow-discharge grids using a JEOL JEE 4B/4C vacuum evaporator? If so, how would one go about doing such? The instruction manual does not cover this. (These would be copper grids filmed with formvar-carbon to be used for negative staining purposes.)
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu http://otocore.wustl.edu
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==============================Original Headers============================== 9, 26 -- From lettj-at-ent.wustl.edu Tue Mar 25 17:02:50 2014 9, 26 -- Received: from wusm-pcf.wustl.edu (pcf-ironmail02.wusm-pcf.wustl.edu [128.252.17.82]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2PM2oFn022388 9, 26 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2014 17:02:50 -0500 9, 26 -- Received: from ([10.39.163.68]) 9, 26 -- by pcf-ironmail02.wusm-pcf.wustl.edu with ESMTP with TLS id 5THYGJ1.26099567; 9, 26 -- Tue, 25 Mar 2014 17:01:32 -0500 9, 26 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 9, 26 -- PCFHUB01.wusm-pcf.wustl.edu ([10.39.163.64]) with mapi id 14.03.0174.001; 9, 26 -- Tue, 25 Mar 2014 17:02:49 -0500 9, 26 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 9, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 26 -- Subject: TEM: glow-discharging grids 9, 26 -- Thread-Topic: TEM: glow-discharging grids 9, 26 -- Thread-Index: Ac9IdWqN1sgIydw5QsK8rgPBedlDXQ== 9, 26 -- Date: Tue, 25 Mar 2014 22:02:47 +0000 9, 26 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E135321EA120-at-pcfmbx01.wusm-pcf.wustl.edu} 9, 26 -- Accept-Language: en-US 9, 26 -- Content-Language: en-US 9, 26 -- X-MS-Has-Attach: 9, 26 -- X-MS-TNEF-Correlator: 9, 26 -- x-originating-ip: [10.39.135.146] 9, 26 -- Content-Type: text/plain; charset="iso-8859-1" 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2PM2oFn022388 ==============================End of - Headers==============================
From pharmacy-affordable15-at-leonet.it Wed Mar 26 08:53:13 2014 Return-Path: {pharmacy-affordable15-at-leonet.it} Received: from host249-pool116.leonet.it (host249-pool116.leonet.it [212.19.116.249] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2QDr9bH017703 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 26 Mar 2014 08:53:10 -0500 Message-Id: {201403261353.s2QDr9bH017703-at-ns.microscopy.com}
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Title-Subject: [Filtered] LaB6 filament life time
Message: Hi everyone, Is there anyone using a LaB6 filament on their SEM... I would like to know what is the filament life time? Is it a lot shorter if we use the filament on the second saturation point?
I currently using a LaB6 filament on a Zeiss EVO and I have to put the Filament Intensity higher and higher everyday to reach the second saturation peak. Do you think my filament is dying or it is another problem? It has 1800 hours lifetime.
Thank you for your help!
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Hi Valerie, I am using LaB6 cathodes since nearly ten years in my Philips / FEI 525 SEM. 1800 hours of beamtime with one filament sounds good. That`s what I get out also, if the vacuum is perfect.
Remember: the cristal tip of the cathode gets sputtered away with time, so near the end of the lifetime you need to adjust the distance tip to wehnelt aperture closer to get the same current. Or you increase (and this is not the best way) the heating current (which you might need to do anyway with this needle-like cristal structure remaining...). Remember also that LaB6 deposits on the wehnelt aperture, leaving an insulating layer you have to clean away.
I would go having a look at your cathode-wehnelt assembly and adjust it new. Then you might be lucky to get some 100 hours of lifetime. Depends how the cathode looks like...
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 27.03.14 02:24, schrieb zaluzec-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: valerie.lecomte-at-usherbrooke.ca () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both valerie.lecomte-at-usherbrooke.ca as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: valerie.lecomte-at-usherbrooke.ca } Name: Valerie Lecomte } } Organization: IOS services geo/Sherbrooke University } } Title-Subject: [Filtered] LaB6 filament life time } } Message: Hi everyone, } Is there anyone using a LaB6 filament on their SEM... } I would like to know what is the filament life time? Is it a lot shorter } if we use the filament on the second saturation point? } } I currently using a LaB6 filament on a Zeiss EVO and I have to put the } Filament Intensity higher and higher everyday to reach the second } saturation peak. Do you think my filament is dying or it is another } problem? It has 1800 hours lifetime. } } Thank you for your help! } } Login Host: 24.37.116.198 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
==============================Original Headers============================== 10, 22 -- From stefan.diller-at-t-online.de Thu Mar 27 03:06:53 2014 10, 22 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2R86rIn020936 10, 22 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 03:06:53 -0500 10, 22 -- Received: from fwd24.aul.t-online.de (fwd24.aul.t-online.de ) 10, 22 -- by mailout08.t-online.de with smtp 10, 22 -- id 1WT5Kt-0004k3-Rp; Thu, 27 Mar 2014 09:06:51 +0100 10, 22 -- Received: from mac-pro.speedport.ip (VsOf10ZOYhgt31Jv8+C34SyQPZWHsucz0gyM8k4m1djK0WCzQiaPlCTiyD1gsk4Q32-at-[91.58.187.74]) by fwd24.t-online.de 10, 22 -- with esmtp id 1WT5Kr-1Q9H280; Thu, 27 Mar 2014 09:06:49 +0100 10, 22 -- Message-ID: {5333DC19.5080806-at-t-online.de} 10, 22 -- Date: Thu, 27 Mar 2014 09:06:49 +0100 10, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 10, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 10, 22 -- MIME-Version: 1.0 10, 22 -- To: microscopy-at-microscopy.com, valerie.lecomte-at-usherbrooke.ca 10, 22 -- Subject: Re: [Microscopy] viaWWW:LaB6 filament life time 10, 22 -- References: {201403270124.s2R1OwXl003200-at-ns.microscopy.com} 10, 22 -- In-Reply-To: {201403270124.s2R1OwXl003200-at-ns.microscopy.com} 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 22 -- Content-Transfer-Encoding: 7bit 10, 22 -- X-ID: VsOf10ZOYhgt31Jv8+C34SyQPZWHsucz0gyM8k4m1djK0WCzQiaPlCTiyD1gsk4Q32 10, 22 -- X-TOI-MSGID: c55afa55-3437-45d1-b9d3-c67239ae8d1d ==============================End of - Headers==============================
From express-meds10-at-lindemulder.org Thu Mar 27 05:19:29 2014 Return-Path: {express-meds10-at-lindemulder.org} Received: from smtp3.lindemulder.org ([85.234.37.106]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2RAJQ68009195 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 27 Mar 2014 05:19:27 -0500 Message-Id: {201403271019.s2RAJQ68009195-at-ns.microscopy.com}
Hi Stefan, et al.,
Kimball Physics has some useful information in their technical notes on the operation of LaB6 cathodes. You can find it on the Kimball Physics web site under Cathodes/Emitters and then Technical information.
The major reason for LaB6 end-of-life is due to loss of brightness of the emitter. Most current emitters are [100] oriented single crystals with a 90 degree cone angle and a roughly 15um flat at the tip. As the tip is used, LaB6 evaporates from it and the tip recedes. There is a faster recession on the [110] faces and as the tip recedes, there is a gradual loss of the flat, i.e. the tip gradually sharpens. Since the emitting area drops, the total current does also. It is also likely that since the cathodes are heated from the edges by the graphite support that the tip is now running cooler that previously.
As an operating note, overheating LaB6 cathodes significantly reduces lifetime. Don't oversaturate! Taking the temperature from the nominal 1800K to 1850K increases the evaporation rate by 3X. This means that the lifetime will go from ~2000hrs to less than 700!
You will see deposits from the cathode on the inside of the wehnelt. These should be a purplish color, indicative of LaB6. If the deposits are white, you have formed Lanthanum oxide. Oxide formation means that the gun vacuum is not adequate. Also, the oxide is an insulator whereas the boride is a semiconductor. This means that as the oxide builds up, you will get charging effects on the inside of the wehnelt. This will eventually result in a flickering beam intensity.
Cheers, Henk
On 3/27/2014 4:08 AM, stefan.diller-at-t-online.de wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Valerie, } I am using LaB6 cathodes since nearly ten years in my Philips / FEI 525 SEM. } 1800 hours of beamtime with one filament sounds good. That`s what I get out also, if the vacuum is perfect. } } Remember: the cristal tip of the cathode gets sputtered away with time, so near the end of the lifetime you need to adjust the } distance tip to wehnelt aperture closer to get the same current. } Or you increase (and this is not the best way) the heating current (which you might need to do anyway with this needle-like } cristal structure remaining...). } Remember also that LaB6 deposits on the wehnelt aperture, leaving an insulating layer you have to clean away. } } I would go having a look at your cathode-wehnelt assembly and adjust it new. Then you might be lucky to get some 100 hours of } lifetime. Depends how the cathode looks like... } } } Best wishes, } Stefan } } } } ----------------------------------------------------- } Stefan Diller - Scientific Photography } Arndtstrasse 22 } D - 97072 Wuerzburg Germany } ++49-931-7848700 Phone } ++49-931-7848701 Fax } ++49-175-7177051 Mobile } } Websites: } www.nanoflight.info } www.stefan-diller.com } www.electronmicroscopy.info } www.elektronenmikroskopie.info } www.zwillingsprojekt.de } www.assisi.de } Anfahrt: http://Mail.map24.com/Stefan.Diller } ----------------------------------------------------- } } Am 27.03.14 02:24, schrieb zaluzec-at-microscopy.com: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } X-from: valerie.lecomte-at-usherbrooke.ca () } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying } } please copy both valerie.lecomte-at-usherbrooke.ca as well as the } } Microscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: valerie.lecomte-at-usherbrooke.ca } } Name: Valerie Lecomte } } } } Organization: IOS services geo/Sherbrooke University } } } } Title-Subject: [Filtered] LaB6 filament life time } } } } Message: Hi everyone, } } Is there anyone using a LaB6 filament on their SEM... } } I would like to know what is the filament life time? Is it a lot shorter } } if we use the filament on the second saturation point? } } } } I currently using a LaB6 filament on a Zeiss EVO and I have to put the } } Filament Intensity higher and higher everyday to reach the second } } saturation peak. Do you think my filament is dying or it is another } } problem? It has 1800 hours lifetime. } } } } Thank you for your help! } } } } Login Host: 24.37.116.198 } } Listserver Email Form V - 20120416 } } --------------------------------------------------------------------------- } } } } } } ==============================Original Headers============================== } 10, 22 -- From stefan.diller-at-t-online.de Thu Mar 27 03:06:53 2014 } 10, 22 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) } 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2R86rIn020936 } 10, 22 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 03:06:53 -0500 } 10, 22 -- Received: from fwd24.aul.t-online.de (fwd24.aul.t-online.de ) } 10, 22 -- by mailout08.t-online.de with smtp } 10, 22 -- id 1WT5Kt-0004k3-Rp; Thu, 27 Mar 2014 09:06:51 +0100 } 10, 22 -- Received: from mac-pro.speedport.ip (VsOf10ZOYhgt31Jv8+C34SyQPZWHsucz0gyM8k4m1djK0WCzQiaPlCTiyD1gsk4Q32-at-[91.58.187.74]) by fwd24.t-online.de } 10, 22 -- with esmtp id 1WT5Kr-1Q9H280; Thu, 27 Mar 2014 09:06:49 +0100 } 10, 22 -- Message-ID: {5333DC19.5080806-at-t-online.de} } 10, 22 -- Date: Thu, 27 Mar 2014 09:06:49 +0100 } 10, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} } 10, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 } 10, 22 -- MIME-Version: 1.0 } 10, 22 -- To: microscopy-at-microscopy.com, valerie.lecomte-at-usherbrooke.ca } 10, 22 -- Subject: Re: [Microscopy] viaWWW:LaB6 filament life time } 10, 22 -- References: {201403270124.s2R1OwXl003200-at-ns.microscopy.com} } 10, 22 -- In-Reply-To: {201403270124.s2R1OwXl003200-at-ns.microscopy.com} } 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 22 -- Content-Transfer-Encoding: 7bit } 10, 22 -- X-ID: VsOf10ZOYhgt31Jv8+C34SyQPZWHsucz0gyM8k4m1djK0WCzQiaPlCTiyD1gsk4Q32 } 10, 22 -- X-TOI-MSGID: c55afa55-3437-45d1-b9d3-c67239ae8d1d } ==============================End of - Headers============================== }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
I think that you are doing very well to keep your filament for 1800 hours. Please be aware that the manufacturers in the past have suggest LaB6 cathode cleaning at least every 500 hours! A cleaner cathode will help the vacuum level in the gun and prolong life.
Be aware that the level of heating with any type of filament will be a big factor in filament life. Are you sure that you must use the settings you mention, could you lower the heating level? But I say again, you have had a pretty good filament life and you are right, you now must consider a replacement?
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0)7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
Organization: IOS services geo/Sherbrooke University
Title-Subject: [Filtered] LaB6 filament life time
Message: Hi everyone, Is there anyone using a LaB6 filament on their SEM... I would like to know what is the filament life time? Is it a lot shorter if we use the filament on the second saturation point?
I currently using a LaB6 filament on a Zeiss EVO and I have to put the Filament Intensity higher and higher everyday to reach the second saturation peak. Do you think my filament is dying or it is another problem? It has 1800 hours lifetime.
Thank you for your help!
Login Host: 24.37.116.198 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Postdoctoral fellowship opportunity in focused ion beam nanotomography at the US Naval Research Laboratory in Washington, DC.
The postdoctoral position is jointly funded by the Office of Naval Research and the NASA Origins program. The focus of the position will be on development of 3D imaging and tomographic reconstruction of materials down to the 10-nm scale, using FIB-SEM slice-and-view methods. Demonstrated skill in the operation of an SEM is required. FIB-SEM and image processing experience, including experience with the Avizo Fire software package, are desirable. The project will involve analysis of meteorites and materials of more direct Navy interest, such as barnacles, or synthetic nanocomposite materials. The salary for this position is set at the NRL/NRC/ASEE fellow rate of $74,872. Interested candidates may send inquiries to rhonda.stroud at nrl.navy.mil. Please include a CV and publication list.
Due to security regulations at NRL, this position requires US citizenship or permanent residency.
Rhonda Stroud
--
___________________________________ Head, Nanoscale Materials Section Naval Research Laboratory 4555 Overlook Ave SW Washington, DC 20375 (V) 202-404-4143 (F) 202-767-1697
==============================Original Headers============================== 8, 18 -- From rhonda.stroud-at-nrl.navy.mil Thu Mar 27 12:56:19 2014 8, 18 -- Received: from ccs.nrl.navy.mil (mx0.ccs.nrl.navy.mil [132.250.118.211]) 8, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2RHuJQw003026 8, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 12:56:19 -0500 8, 18 -- Received: from [IPv6:::1] (blue-vaio.nrl.navy.mil [IPv6:2001:480:20:126:5642:49ff:fe97:afb6]) 8, 18 -- by ccs.nrl.navy.mil (8.14.4/8.14.4) with ESMTP id s2RHuHjc025226 8, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 13:56:18 -0400 8, 18 -- Message-ID: {53346637.7070606-at-nrl.navy.mil} 8, 18 -- Date: Thu, 27 Mar 2014 13:56:07 -0400 8, 18 -- From: Rhonda Stroud {rhonda.stroud-at-nrl.navy.mil} 8, 18 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 8, 18 -- MIME-Version: 1.0 8, 18 -- To: Microscopy-at-microscopy.com 8, 18 -- Subject: Postdoc opportunity -- FIB tomography at NRL in Washington, DC 8, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 18 -- Content-Transfer-Encoding: 7bit 8, 18 -- X-CCS-MailScanner: No viruses found. 8, 18 -- X-CCS-MailScanner-Info: See: http://www.nrl.navy.mil/ccs/support/email ==============================End of - Headers==============================
A postdoctoral fellowship position is available now at the Naval Research Laboratory in Washington, DC, for the study of the mineralogy and petrology of meteorites and returned samples. The successful candidate will use STEM-based spectroscopy for mineralogical studies, including oxidation state analysis. The postdoc will coordinate the STEM studies with synchrotron-based studies conducted by team members at the partnering NASA SSERVI RIS^4 E institutions, for the analysis of analog materials, meteorites and returned lunar, Stardust and/or Hayabusa samples. Expertise in transmission electron microscopy is required, and familiarity with EELS and advanced sample preparation methods is preferred. Instrumentation at NRL for these studies includes a Nion UltraSTEM 200 (to be installed June 2014), and a JEOL 2200FS. The salary for this position is set at the NRL/NRC/ASEE fellow rate of $74,872. Interested candidates may send inquiries to rhonda.stroud at nrl.navy.mil {mailto:rhonda.stroud-at-nrl.navy.mil} . Please include a CV and publication list.
Due to security regulations at NRL, this position requires US citizenship or permanent residency.
Rhonda Stroud
--
___________________________________ Head, Nanoscale Materials Section Naval Research Laboratory 4555 Overlook Ave SW Washington, DC 20375 (V) 202-404-4143 (F) 202-767-1697
==============================Original Headers============================== 7, 19 -- From rhonda.stroud-at-nrl.navy.mil Thu Mar 27 13:40:29 2014 7, 19 -- Received: from ccs.nrl.navy.mil (mx0.ccs.nrl.navy.mil [132.250.118.211]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2RIeTAq022373 7, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 13:40:29 -0500 7, 19 -- Received: from [IPv6:::1] (blue-vaio.nrl.navy.mil [IPv6:2001:480:20:126:5642:49ff:fe97:afb6]) 7, 19 -- by ccs.nrl.navy.mil (8.14.4/8.14.4) with ESMTP id s2RIeSMC023053 7, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 14:40:29 -0400 7, 19 -- Message-ID: {53347092.30107-at-nrl.navy.mil} 7, 19 -- Date: Thu, 27 Mar 2014 14:40:18 -0400 7, 19 -- From: Rhonda Stroud {rhonda.stroud-at-nrl.navy.mil} 7, 19 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 7, 19 -- MIME-Version: 1.0 7, 19 -- To: Microscopy-at-microscopy.com 7, 19 -- Subject: Postdoc opportunity STEM-EELS of planetary materials at NRL, Washington, 7, 19 -- DC 7, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 19 -- Content-Transfer-Encoding: 7bit 7, 19 -- X-CCS-MailScanner: No viruses found. 7, 19 -- X-CCS-MailScanner-Info: See: http://www.nrl.navy.mil/ccs/support/email ==============================End of - Headers==============================
" If I compare the effect of product A and B (whatever effect it doesn't matter) and I find with the Student-t test that p (A)=0,04 and p (B)=0,005, may I say that B works better than A?"
Disclaimer - I'm not a statistician, just a heavy user of stats.
My long-winded way of saying No:
The Student t-test [1,2] probably used is better described as a test of the means of two normal distributions with variances unknown, but equal [3]. It assumes data are univariate, continuous, and normal distributed, and data are collected randomly [3, 4]. It should not be used when data are ranked, not approximately normally distributed, and more than two groups are to be compared [4]. It is not valid for multiple comparisons (because of additive errors) or where group sizes are very unequal [4]. The test is robust for moderate departures from normality, and when N1 and N2 are approximately equal it is robust from departures of homogeneity (equality) of variances [3,4]. An F-test is commonly performed to check the homogeneity of the data (determine if the variances are equal). If they are homogeneous, then for comparing the Mean of two groups, the t-test can be used. If they are heterogeneous, then a modified t-test (Cochran t-test; test of the means of two normal distributions with variances unknown, but unequal) [3-5] can be used. If the data are not normally distributed, then a non-parametric Student's test could be performed [6]. In addition, "although one might presume that a small P value indicates the presence of strong evidence against the null, such is not necessarily the case" [7], e.g., a better p-value doesn't mean better test method/group. Since it is a test of the Means (is this data set different from another), it says very little about the rest of the data spread, which may be more important.
If one wishes to compare more than two groups (Control, A, and B), then ANOVA is usually performed. The typical use of ANOVA is to demonstrate both a difference and through post-hoc analysis a significance for treatment sets (Class I and II uses according to Eisenhart [8]) [4]. The use of ANOVA is predicated on the assumption of normality [8, 9], with varying consequences in both Class I and Class II uses [9]. A common example of a non-normal data set would be counting, such fiber or mineral grain or blood cell counting as these better following a binomial-based distribution - poisson or negative binomial [10-19]. In these cases, the count data should be transformed by taking the square root to "normalize" the data [20-22] prior to performing ANOVA. Even if ANOVA is used, the strength of the effect should still be looked at carefully/cautiously [23-25]. Furthermore, a different multi-comparison method may be more appropriate [26-30].
1. Student: Probable Error of a Correlation Coefficient, Biometrika, 6, 2-3, 302-310, 1908. (pen name for Gosset) 2. Student: The Probable Error of a Mean, Biometrika, 6, 1, 1-25, 1908. 3. Hines, Wulliam H., and Douglas C. Montgomery: Probability and Statistics in Engineering and Management Science, 3rd ed. John Wiley & Sons, NY. NY. 1990. 4. Gad, Shayne, and Carrol S. Weil: Statistics and Experimental Design for Toxicologists, 2nd Ed. CRC Press, Boca Raton, FL. 1991. 5. Cochran, William G., Gertrude M. Cox: Experimental Designs, 2nd ed. John Wiley & Sons, NY, NY. 1975. 6. Walsh, John E.: Some Nonparametric Tests for Student's Hypothesis in Experimental Designs, J Am Stat Assoc, 47, 259, 401-415, 1952. 7. Berger, James, and Thomas Sellke Testing a Point Null Hypothesis, The Irreconcilability of P Values and Evidence, J Am Stat Assoc, 82, 397, 112-122, 1987. 8. Eisenhart, The Assumptions Underlying the Analysis of Variance, Biometrics, 3, 1, 1-21, 1947. 9. Cochran, Some Consequences When the Assumptions for the Analysis of Variance are not Satisfied, Biometrics, 3, 1, 22-38), 1947. 10. NIOSH: Proficiency Analytical Testing Program & Environmental Lead Proficiency Analytical Testing Program (PAT & ELPAT) Programs. NIOSH Pub 95-104. USDHHS, Cincinnati, OH. November, 1994. 11. Schlect, P.C., and S.A. Shulman: Performance of asbestos fiber counting laboratories in the NIOSH proficiency analytical testing (PAT) program. AIHAJ 47(5):259-269. 1986. 12. Reist, A Further Note on the Reliability of Membrane Filter Dust Sample Evaluation by Microscope Counting, Ann Occ Hyg, 13, 4, 201-204, 1970. 13. Hoyes, A Routine for the Control of Perform of Microscopists Eval Airborne Asbestos Fibre Samples on Membrane Filters by PCM, Ann Occ Hyg, 23, 4, 381-401, 1980 14. Sniegowski, A Note on Reliability of Membrane Filter Dust Sample Evaluation by Microscope Counting, Ann Occ Hyg, 9, 2, 65-67, 1966. 15. Chapman, Harlow M., and Russell C. Ruhf: Dust Counting Reliability, AIHA Qtr, 16, 3, 210-209, 1955. 16. Reid, George W.: Calculation of Counting Statistics, AIHA Qtr, 13, 1, 29-32, 1952. 17. Oehlert, Statistical Anal of Asbestos Fibre Counts, Environmetrics, 6, 115-126, 1995. 18. Chayes, Felix: Petrographic Modal Analysis. John Wiley & Sons, NY. NY. 1956. 19. Student: On the Error of Counting with a Haemacytometer, Biometrika, 5, 3, 351-360, 1907. 20. Barlett, M.S.: The Square Root Transformation in Analysis of Variance, Suppl to J Roy Stat Soc, 3, 1, 68-78, 1936. 21. Bartlett, M.S.: The Use of Transformations, Biometrics, 3, 1, 39-52, 1947. 22. Havics, A: Asbestos Fiber Counting by Different optical techniques, Microscope, 58, 2, 51-62, 2010. 23. Ronis, David L.: Comparing the Magnitude of Effects in Anova Designs, Ed Psychol Meas, 41, 4, 993-1000, 1981. 24. Vaughan, Graham, and Michael C. Corbalis: Beyond Tests of Significance, Est Strength of Effects in Slected ANOVA Design, Psychol Bull, 72, 3, 204-213, 1969. 25. Camilli, Gregory, and Lorrie A. Shepard: The inadequacy of ANOVA for detecting test bias, J Ed Stats, 12, 1, 87099, 1987. 26. Riegelman, Richard K.: Studying a Study and Testing a Test, 5th ed. Lippincott Williams & Wilkins. 2005. 27. Shaffer, Juliet Popper: Multiple hypothesis testing, Ann Rev Psychol, 46, 561-584, 1995 28. Holland, Burt, and Margaret DiPonzio Copenhaver: Improved Bonferroni-Type Multiple Testing Procedures, Physcol Bull, 104, 1, 145-149, 1988 29. Einot, Israeil, and K.R. Gabrie: A study of the powers of several methods of multiple comparisons, J Am Stat Assoc, 70, 351, 574-583, 1975. 30. Curran-Everett, Douglas: Multiple comparisons, philosophies and illustrations, Am J Physiol Reg Integ Comp Physiol, 279, R1-R-8, 2000.
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-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, March 25, 2014 5:59 AM To: ph2-at-sprynet.com
Dear colleagues, i have a question regarding the analysis of statistics, I apologize if this is not directly related to microscopy but I know that some of you are hardcore statisticians and I already got help in the past. If I compare the effect of product A and B (whatever effect it doesn't matter) and I find with the Student-t test that p (A)=0,04 and p (B)=0,005, may I say that B works better than A? I always thought that this interpretation of statistics is not allowed but I now see it in a recent Science paper and I surprised. Many thanks in advance. Stephane
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A recent reply to this thread was rather technical and dense. In contrast "Naked Statistics" by Charles Wheelan, which I have just finished reading, is an easy read and an accessible guide to the power and traps of doing statistical analysis.
It might be a good place to gain perspective.
Alwyn Eades Department of Materials Science and Engineering Lehigh University
==============================Original Headers============================== 4, 20 -- From jae5-at-lehigh.edu Thu Mar 27 14:52:52 2014 4, 20 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2RJqqK9028676 4, 20 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 14:52:52 -0500 4, 20 -- Received: from [192.168.1.117] (24.102.248.186.res-cmts.hel.ptd.net [24.102.248.186]) 4, 20 -- (authenticated bits=0) 4, 20 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id s2RJqi1U027120 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT) 4, 20 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 15:52:52 -0400 4, 20 -- Message-ID: {53348184.8050003-at-lehigh.edu} 4, 20 -- Date: Thu, 27 Mar 2014 15:52:36 -0400 4, 20 -- From: Alwyn Eades {jae5-at-lehigh.edu} 4, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 4, 20 -- MIME-Version: 1.0 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- Subject: RE: Fw: overinterpretation of statistics 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- X-Virus-Scanned: clamav-milter 0.98 at rain.cc.lehigh.edu 4, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I find "Intuitive Biostatistics" by Harvey Motulsky as quite accessible for the statistical novice. He is the guy behind Graphpad statistical software.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: jae5-at-lehigh.edu [mailto:jae5-at-lehigh.edu] Sent: Thursday, March 27, 2014 2:53 PM To: Phillips, Thomas E.
A recent reply to this thread was rather technical and dense. In contrast "Naked Statistics" by Charles Wheelan, which I have just finished reading, is an easy read and an accessible guide to the power and traps of doing statistical analysis.
It might be a good place to gain perspective.
Alwyn Eades Department of Materials Science and Engineering Lehigh University
==============================Original Headers============================== 4, 20 -- From jae5-at-lehigh.edu Thu Mar 27 14:52:52 2014 4, 20 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2RJqqK9028676 4, 20 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 14:52:52 -0500 4, 20 -- Received: from [192.168.1.117] (24.102.248.186.res-cmts.hel.ptd.net [24.102.248.186]) 4, 20 -- (authenticated bits=0) 4, 20 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id s2RJqi1U027120 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT) 4, 20 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 15:52:52 -0400 4, 20 -- Message-ID: {53348184.8050003-at-lehigh.edu} 4, 20 -- Date: Thu, 27 Mar 2014 15:52:36 -0400 4, 20 -- From: Alwyn Eades {jae5-at-lehigh.edu} 4, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 4, 20 -- MIME-Version: 1.0 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- Subject: RE: Fw: overinterpretation of statistics 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- X-Virus-Scanned: clamav-milter 0.98 at rain.cc.lehigh.edu 4, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 14, 30 -- From PhillipsT-at-missouri.edu Thu Mar 27 15:38:33 2014 14, 30 -- Received: from mst-rip6-missouri-out.um.umsystem.edu (mst-rip6-missouri-out.um.umsystem.edu [198.209.50.149]) 14, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2RKcVS8017802 14, 30 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 15:38:32 -0500 14, 30 -- X-IronPort-Anti-Spam-Filtered: true 14, 30 -- X-IronPort-Anti-Spam-Result: AkgFAEGLNFPPoJ7G/2dsb2JhbABWA4MGO1esGJZFQF8WdIIlAQEBBDoRAQIeCg8CAgIBCBECAQEBAQsCEgkHFg8MARQDAQUIAgQKCQgVBIdYDbNjAZcThlQXBIx1gTMdIRIFBguDE4EUBJoAkQGDL4Ir 14, 30 -- X-IPAS-Result: AkgFAEGLNFPPoJ7G/2dsb2JhbABWA4MGO1esGJZFQF8WdIIlAQEBBDoRAQIeCg8CAgIBCBECAQEBAQsCEgkHFg8MARQDAQUIAgQKCQgVBIdYDbNjAZcThlQXBIx1gTMdIRIFBguDE4EUBJoAkQGDL4Ir 14, 30 -- Received: from um-tcas2.um.umsystem.edu ([207.160.158.198]) 14, 30 -- by mst-rip6-exch-relay.um.umsystem.edu with ESMTP; 27 Mar 2014 15:38:31 -0500 14, 30 -- Received: from UM-MBX-N02.um.umsystem.edu ([169.254.5.16]) by 14, 30 -- UM-TCAS2.um.umsystem.edu ([207.160.158.198]) with mapi id 14.03.0181.006; 14, 30 -- Thu, 27 Mar 2014 15:38:30 -0500 14, 30 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 14, 30 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 14, 30 -- Subject: RE: [Microscopy] RE: Fw: overinterpretation of statistics 14, 30 -- Thread-Topic: [Microscopy] RE: Fw: overinterpretation of statistics 14, 30 -- Thread-Index: AQHPSfY0bzetvNa610CPt2WZtsXGXZr1ZKzA 14, 30 -- Date: Thu, 27 Mar 2014 20:38:30 +0000 14, 30 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DDBD60BC2-at-UM-MBX-N02.um.umsystem.edu} 14, 30 -- References: {201403271953.s2RJrHiZ029528-at-ns.microscopy.com} 14, 30 -- In-Reply-To: {201403271953.s2RJrHiZ029528-at-ns.microscopy.com} 14, 30 -- Accept-Language: en-US 14, 30 -- Content-Language: en-US 14, 30 -- X-MS-Has-Attach: 14, 30 -- X-MS-TNEF-Correlator: 14, 30 -- x-originating-ip: [207.160.158.206] 14, 30 -- Content-Type: text/plain; charset="us-ascii" 14, 30 -- MIME-Version: 1.0 14, 30 -- Content-Transfer-Encoding: 8bit 14, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2RKcVS8017802 ==============================End of - Headers==============================
Just a reminder about next Wednesday's webinar: Brightfield Color imaging: Is what you saw what you get? Wednesday, April 2, 2014, 1:00PM EDT To register: {http://scientific.datacolor.com/resources/events/} http://scientific.datacolor.com/resources/events/
Guest Speaker: Dr. Eduardo Rosa-Molinar Dr. Rosa-Molinar is a Professor in the Department of Biology at the University of Puerto Rico Rio Piedras. He serves on the FASEB Board of Directors, and has served in numerous positions with The Histochemical Society, the Society for Integrative and Comparative Biology, the Society for Neuroscience, and is currently the Chair of the Andor/Bitplane Advanced Imaging Centers.
Interpretation and communication are the essence of science. But what happens when what you see in the microscope is not what you see on your monitor? Or your laptop? Or that your colleague sees when he opens that all-important image you sent him? Ironically, these variations in the image are often most pronounced in that good old workhorse, brightfield imaging of stained samples. This webinar will review best practices in brightfield imaging then discuss how Datacolor's ChromaCal microscope and monitor systems can help you to standardize those images so that what you saw in the microscope is what you get on your screen.
==============================Original Headers============================== 7, 35 -- From bfoster-at-the-mip.com Thu Mar 27 17:06:56 2014 7, 35 -- Received: from nm2-vm5.access.bullet.mail.bf1.yahoo.com (nm2-vm5.access.bullet.mail.bf1.yahoo.com [216.109.114.92]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2RM6tdC008802 7, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 17:06:56 -0500 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1395958015; bh=NwNx/K5xiHdUglS46c4hGLFiG6h9YCybG46ztZ9n9VU=; h=Received:Received:Received:X-Yahoo-Newman-Id:Message-ID:X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:X-Mailer:Date:To:From:Subject:Mime-Version:Content-Type; b=pUYYT+WvjsJ/zi7jb7L800O8jIQeYM5e9fUOL0RMxQrRkAn4RI+GOUWg/gGz/f8/6u+4OF7Y+s1inxTe2JI62nxXW0wAjhwimU0mQgSwQ9g63rTTLJlMt8hkBG0G+SlHEkANX9EdZ2IJJROjEg/HcVBZtugdmaZqbXej13gLL3mzZaa8T2dJUDZTthCeEfS9ka2iiz6VoHlBrx0CSMeunNe5TQVVvl2iVsV/67kZaxzzBRE4JyIVr14IgzPCPA9LZL5F2WPyvHtIr0LTGDqS4P52X1PKoFQWfHLVqOexKevjIKwUGPSULGJ6WgK/Gk/HgQtkiOrqaie14SOZw/EKgw== 7, 35 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s2048; d=yahoo.com; 7, 35 -- b=WgbljO3FJFCgNn2Fcjv1dmYkG03CvDUDJ5J67laDhybwxGMq2O/wiB5AQ/aVJzqzGWdFrwJT0KzlOFVs2/fn0bX9R3HSOiELbAyguQ8p0CxAI3rhXST6DERu65D/d/al3lcOyeTlsynnA+OjKq3kbVB09ANZbWYmpYzXHjj9OoS5eMUkrnopTwomwcC1h3QKcM9VS2Frjd8kFhgfitVIk6etoeTfLWmDFtEobOHV4xXf4OQeQTNXKbZf72aQamYbu9imAOzzWdx0QxZGSUfLC3pRAusFNRFiwDkDoFb+PVnBy+qbpS3Rebrla0NiMQKGELtmhUdOAYy9ErKFtzsVHQ==; 7, 35 -- Received: from [66.196.81.166] by nm2.access.bullet.mail.bf1.yahoo.com with NNFMP; 27 Mar 2014 22:06:55 -0000 7, 35 -- Received: from [98.138.226.240] by tm12.access.bullet.mail.bf1.yahoo.com with NNFMP; 27 Mar 2014 22:06:55 -0000 7, 35 -- Received: from [127.0.0.1] by smtp111.sbc.mail.ne1.yahoo.com with NNFMP; 27 Mar 2014 22:06:55 -0000 7, 35 -- X-Yahoo-Newman-Id: 257246.18793.bm-at-smtp111.sbc.mail.ne1.yahoo.com 7, 35 -- Message-ID: {257246.18793.bm-at-smtp111.sbc.mail.ne1.yahoo.com} 7, 35 -- X-Yahoo-Newman-Property: ymail-3 7, 35 -- X-YMail-OSG: iE77rnoVM1ms2c_1JAJ.jNHCEEkcUlxPT_lBo1.PODY3Eav 7, 35 -- cwN9USf5E_NunAL.96RAwvXP5Phze3s1ConXck0zPSpgyBvJfAGs6qK4wWwJ 7, 35 -- Vw5DGNpgry80Vvlol_aX24xAv.lC2WGkVDxAXVXNezo1w6CHSurHkOaaZ5TD 7, 35 -- dhK5f55gqqifJKBAOzELHt3e0DTAveNawuercvnoFZKfnrhDW5zFFbT0fGFz 7, 35 -- SFreQ8h8jx619m2ieLYrPTy4g6vbyAkGwOjwu4IbAnOD2S_MlaDRu7ZWFUrs 7, 35 -- SUMLsZMT8qjGi8Bni.6gG35bRwQb.PX0pp2zk8qP8671_NVprDbtZ_jdl87c 7, 35 -- H75QvqF_0ofX4wQoI4.NDMFQ9qlAa09HQXXG.LrxIVVn37wccG6UcwnKRSwZ 7, 35 -- 0guaa4kvHi3ttKnlNQkdRV3moBwTmmrvvFJjndRsQc.f5OGlT8J1BpCw43lO 7, 35 -- sdpNfluWPhATQmdl3WZvahECn3YuZ2xIuSrYWBzWEVqmMyKuh9QGOWmT7noA 7, 35 -- XSvsLgu6vJG9f3jx4R17Lubst93PDH6ph0ub.nUur8LAoKKYP5M4ebq2qLDn 7, 35 -- pCuaXSFZ6JwhNGXFiIOfw_bdneUJ7SXpQpbgYyrIPq5WB36tzG2oNCsxlSOY 7, 35 -- xiIHko13N1yKVYNu85V8EKRhQ4mqvxy4orA-- 7, 35 -- X-Yahoo-SMTP: KOmzyESswBATZkB2214rZDIfSSNffJ2urJX_LO8NW63iukNVtw-- 7, 35 -- X-Rocket-Received: from Bs-PC.the-mip.com (bfoster-at-99.168.105.118 with plain [98.138.84.52]) 7, 35 -- by smtp111.sbc.mail.ne1.yahoo.com with SMTP; 27 Mar 2014 15:06:55 -0700 PDT 7, 35 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 35 -- Date: Thu, 27 Mar 2014 17:06:33 -0500 7, 35 -- To: Microscopy-at-microscopy.com 7, 35 -- From: Barbara Foster {bfoster-at-the-mip.com} 7, 35 -- Subject: Webinar Reminder: ChromaCal Brightfield Color Image Calibration 7, 35 -- Mime-Version: 1.0 7, 35 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
From drugstore-express6-at-corbina.ru Fri Mar 28 02:08:55 2014 Return-Path: {drugstore-express6-at-corbina.ru} Received: from 93-81-36-180.broadband.corbina.ru (93-81-36-180.broadband.corbina.ru [93.81.36.180]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2S78qFS011549 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 28 Mar 2014 02:08:53 -0500 Message-Id: {201403280708.s2S78qFS011549-at-ns.microscopy.com}
After seeing these prestigious references, I'm guess I shouldn't bother mentioning "Statistics for Dummies" by Rumsey.
Opps! Too late..........
Stay safe Frank
-----Original Message----- X-from: jae5-at-lehigh.edu [mailto:jae5-at-lehigh.edu] Sent: Thursday, March 27, 2014 4:01 PM To: Frank Karl
A recent reply to this thread was rather technical and dense. In contrast "Naked Statistics" by Charles Wheelan, which I have just finished reading, is an easy read and an accessible guide to the power and traps of doing statistical analysis.
It might be a good place to gain perspective.
Alwyn Eades Department of Materials Science and Engineering Lehigh University
==============================Original Headers============================== 4, 20 -- From jae5-at-lehigh.edu Thu Mar 27 14:52:52 2014 4, 20 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2RJqqK9028676 4, 20 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 14:52:52 -0500 4, 20 -- Received: from [192.168.1.117] (24.102.248.186.res-cmts.hel.ptd.net [24.102.248.186]) 4, 20 -- (authenticated bits=0) 4, 20 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id s2RJqi1U027120 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT) 4, 20 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2014 15:52:52 -0400 4, 20 -- Message-ID: {53348184.8050003-at-lehigh.edu} 4, 20 -- Date: Thu, 27 Mar 2014 15:52:36 -0400 4, 20 -- From: Alwyn Eades {jae5-at-lehigh.edu} 4, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 4, 20 -- MIME-Version: 1.0 4, 20 -- To: microscopy-at-microscopy.com 4, 20 -- Subject: RE: Fw: overinterpretation of statistics 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- X-Virus-Scanned: clamav-milter 0.98 at rain.cc.lehigh.edu 4, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
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==============================Original Headers============================== 17, 26 -- From frank_karl-at-ardl.com Fri Mar 28 06:54:27 2014 17, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2SBsQup001477 17, 26 -- for {microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 06:54:26 -0500 17, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 17, 26 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Fri, 28 17, 26 -- Mar 2014 07:54:26 -0400 17, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 17, 26 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 17, 26 -- {microscopy-at-microscopy.com} 17, 26 -- Date: Fri, 28 Mar 2014 07:54:25 -0400 17, 26 -- Subject: was overinterpretation of statistics-- now books 17, 26 -- Thread-Topic: was overinterpretation of statistics-- now books 17, 26 -- Thread-Index: Ac9J92NwF0RYDFz5QuuILZhIZpX9tgAhLK4g 17, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BE8DC55C9-at-exchange2k7.ad.ardl.com} 17, 26 -- References: {201403272001.s2RK16sh010324-at-ns.microscopy.com} 17, 26 -- In-Reply-To: {201403272001.s2RK16sh010324-at-ns.microscopy.com} 17, 26 -- Accept-Language: en-US 17, 26 -- Content-Language: en-US 17, 26 -- X-MS-Has-Attach: 17, 26 -- X-MS-TNEF-Correlator: 17, 26 -- acceptlanguage: en-US 17, 26 -- Content-Type: text/plain; charset="us-ascii" 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2SBsQup001477 ==============================End of - Headers==============================
The Northern California Society for Microscopy Spring Meeting, featuring presentations by Kent McDonald and Ryan McGorty, will be held Thursday, April 17, 2014 6:00 PM to 9:00 PM.
Please RSVP to info-at-ncsmicroscopy.org
Scotts of Palo Alto #1 Town and Country Village Palo Alto, California http://scottsseafoodpa.com/
Featured Speakers Are:
Kent McDonald, PhD, Electron Microscopy Lab, UC Berkeley
Rapid Specimen Processing Methods for Cryofixed Samples
New methods for freeze substitution have been developed that require only basic laboratory tools. Excellent freeze substitution results can be obtained in as little as 90min for cells of small volume. Rapid epoxy resin infiltration can be achieved by centrifugation followed by polymerization at 100 C for 1.5–2 h. Total processing time from freezing to blocks ready to section is about 6 h. Comparative results will be presented.
Ryan McGorty, PhD, UC San Francisco, School of Pharmacy
Three-dimensional super-resolution imaging of tissue samples
Single-molecule localization microscopy methods are often used to study samples at or very near the coverslip surface with a spatial resolution of around 20nm. However, a number of challenges emerge when trying to image even a few microns past the coverslip surface, particularly when trying to accurately localize molecules in 3D. After first explaining the principle of the super-resolution microscopy technique we use, I will discuss multiple methods we have recently developed that allow us to image deeper into tissue samples.
No Cost to Current Members. $25 for Non-Members.
Bring your check, payable to NCSM, for 2014 dues and enjoy a 3-course meal and great speakers. Annual dues for regular members are $40; students, $20; and a corporate membership (which includes one person) is $100, payable to "NCSM"
_______________________ Roseann Csencsits, PhD Scientist in Charge, Downing TEM Tools Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
==============================Original Headers============================== 33, 44 -- From rcsencsits-at-lbl.gov Fri Mar 28 10:57:27 2014 33, 44 -- Received: from fe1.lbl.gov (fe1.lbl.gov [128.3.41.133]) 33, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2SFvRLW015126 33, 44 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 10:57:27 -0500 33, 44 -- X-Ironport-SBRS: 4.8 33, 44 -- X-IronPort-Anti-Spam-Filtered: true 33, 44 -- X-IronPort-Anti-Spam-Result: As8BAJGbNVPRVaAulGdsb2JhbABWAw6DM8RdFg4BAQEBBwsLCRIqg0xuAQUBIxKHeQWicI4YoGcXjxKDE4EUBIlSjRKDHYUaiXlBhB5g 33, 44 -- X-IronPort-AV: E=Sophos;i="4.97,751,1389772800"; 33, 44 -- d="scan'208";a="44331013" 33, 44 -- Received: from mail-pb0-f46.google.com ([209.85.160.46]) 33, 44 -- by fe1.lbl.gov with ESMTP; 28 Mar 2014 08:57:26 -0700 33, 44 -- Received: by mail-pb0-f46.google.com with SMTP id rq2so5175526pbb.19 33, 44 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 08:57:26 -0700 (PDT) 33, 44 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 33, 44 -- d=1e100.net; s=20130820; 33, 44 -- h=x-gm-message-state:subject:from:content-type:message-id:date:to 33, 44 -- :content-transfer-encoding:mime-version; 33, 44 -- bh=SxqXXUAQ9591spW/dbP6stgAtEUeehPAy3Gvuo/M3mc=; 33, 44 -- b=O/fjBZ1ZcAEvjNkEaLCOmblns1GA3+JfC142d20xLr3MlAExP9vubwtan7pz1+C5bg 33, 44 -- OZZRPYONkjrP45H/VAd0QK2U/03GBsLx/cbqV9bXmouHp4KrcdbSCu+du5Sv8+EH0dy0 33, 44 -- 3dZUhogVw/M5WV5OUdoIWWRWCKtUt2cVzGUTTqk8o2XQn2SwdUdDBnWJ3hRAcIns5lP1 33, 44 -- DHm+HrEdr/DqBqCiep3unPpWUG0DIjMHxPugpGPqaFkCLA+ggccNdRJso+Qd0uFwuTqE 33, 44 -- yCFag4W+h6lXEDisk/WdyWEp7Ws9KflZzMUpoJt4iTVEC3U7J48yd9fxmjLpx1USYkMW 33, 44 -- pvYw== 33, 44 -- X-Gm-Message-State: ALoCoQlVgg6UrVvqJffz8qWowymNkRNdgbDrFAgimEVktFsrGrgo7qXUAcOO7UywgD9+yw5xzpEunD/IJgIncyjAetVFJz4/6GQD4DekmpKKwSK97g4r0w3/tJimW0K/D8iYMRPSI2/UEuKKuzjeDp5Qe81aj6GlmfPPUnjTicbMABIiizfT7BQ= 33, 44 -- X-Received: by 10.68.89.162 with SMTP id bp2mr9360699pbb.151.1396022246607; 33, 44 -- Fri, 28 Mar 2014 08:57:26 -0700 (PDT) 33, 44 -- X-Received: by 10.68.89.162 with SMTP id bp2mr9360682pbb.151.1396022246385; 33, 44 -- Fri, 28 Mar 2014 08:57:26 -0700 (PDT) 33, 44 -- Received: from [131.243.35.46] (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov. [131.243.35.46]) 33, 44 -- by mx.google.com with ESMTPSA id c7sm24761343pbt.0.2014.03.28.08.57.25 33, 44 -- for {multiple recipients} 33, 44 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 33, 44 -- Fri, 28 Mar 2014 08:57:25 -0700 (PDT) 33, 44 -- Subject: Northern California Society for Microscopy 2014 Spring Meeting 33, 44 -- From: Roseann Csencsits {rcsencsits-at-lbl.gov} 33, 44 -- Content-Type: text/plain; charset=windows-1252 33, 44 -- Message-Id: {F5ECF10C-6A7D-4DCB-BADF-D9C6789C89A1-at-lbl.gov} 33, 44 -- Date: Fri, 28 Mar 2014 07:54:53 -0700 33, 44 -- To: Microscopy-at-microscopy.com, 3dem-at-ncmir.ucsd.edu 33, 44 -- Mime-Version: 1.0 (Apple Message framework v1085) 33, 44 -- X-Mailer: Apple Mail (2.1085) 33, 44 -- Content-Transfer-Encoding: 8bit 33, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2SFvRLW015126 ==============================End of - Headers==============================
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Fri Mar 28 13:28:27 2014 4, 20 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2SISQOi003633 4, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 13:28:26 -0500 4, 20 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id s2SISMVt001539 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 08:28:22 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id s2SISM98001536 4, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 08:28:22 -1000 (HST) 4, 20 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Fri, 28 Mar 2014 08:28:22 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-sf-v240 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: Etching a castle onto a grain of sand with FIB/SEM 4, 20 -- Message-ID: {Pine.GSO.4.64.1403280826090.4130-at-sf-v240} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Interesting. But why an operator was in latex gloves? Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu] Sent: Friday, March 28, 2014 1:29 PM To: Dusevich, Vladimir
I was all excited when I heard someone on this campus is going to get a FIB/SEM, but I never imagined this...
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Fri Mar 28 13:28:27 2014 4, 20 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2SISQOi003633 4, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 13:28:26 -0500 4, 20 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id s2SISMVt001539 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 08:28:22 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id s2SISM98001536 4, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 08:28:22 -1000 (HST) 4, 20 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Fri, 28 Mar 2014 08:28:22 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-sf-v240 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: Etching a castle onto a grain of sand with FIB/SEM 4, 20 -- Message-ID: {Pine.GSO.4.64.1403280826090.4130-at-sf-v240} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 12, 30 -- From DusevichV-at-umkc.edu Fri Mar 28 15:44:53 2014 12, 30 -- Received: from mst-rip6-umkc-out.um.umsystem.edu (mst-rip6-umkc-out.um.umsystem.edu [198.209.50.152]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2SKirFT023949 12, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2014 15:44:53 -0500 12, 30 -- X-IronPort-Anti-Spam-Filtered: true 12, 30 -- X-IronPort-Anti-Spam-Result: AkoFAJTeNVPPoJ7E/2dsb2JhbABWA4MGO1e7QIc1gRgWdIIlAQEBBDoUNwQCAQgRAwEBAQsCEgkHMQEUCQgCBBMIFQSHWA3LPYZYF45JBhsSBQYLgh12gRQEpTyFR4Mwgis 12, 30 -- X-IPAS-Result: AkoFAJTeNVPPoJ7E/2dsb2JhbABWA4MGO1e7QIc1gRgWdIIlAQEBBDoUNwQCAQgRAwEBAQsCEgkHMQEUCQgCBBMIFQSHWA3LPYZYF45JBhsSBQYLgh12gRQEpTyFR4Mwgis 12, 30 -- Received: from um-tcas1.um.umsystem.edu ([207.160.158.196]) 12, 30 -- by mst-rip6-exch-relay.um.umsystem.edu with ESMTP; 28 Mar 2014 15:44:52 -0500 12, 30 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.83]) by 12, 30 -- UM-TCAS1.um.umsystem.edu ([207.160.158.196]) with mapi id 14.03.0181.006; 12, 30 -- Fri, 28 Mar 2014 15:44:52 -0500 12, 30 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 12, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 30 -- Subject: RE: [Microscopy] Etching a castle onto a grain of sand with FIB/SEM 12, 30 -- Thread-Topic: [Microscopy] Etching a castle onto a grain of sand with FIB/SEM 12, 30 -- Thread-Index: AQHPSrOcrLOE+qP7Qkyrho8zuEOx35r295PQ 12, 30 -- Date: Fri, 28 Mar 2014 20:44:51 +0000 12, 30 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DBB493F-at-UM-MBX-T01.um.umsystem.edu} 12, 30 -- References: {201403281829.s2SIT6HK004297-at-ns.microscopy.com} 12, 30 -- In-Reply-To: {201403281829.s2SIT6HK004297-at-ns.microscopy.com} 12, 30 -- Accept-Language: en-US 12, 30 -- Content-Language: en-US 12, 30 -- X-MS-Has-Attach: 12, 30 -- X-MS-TNEF-Correlator: 12, 30 -- x-originating-ip: [134.193.156.218] 12, 30 -- Content-Type: text/plain; charset="us-ascii" 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s2SKirFT023949 ==============================End of - Headers==============================
From drugs_canadian3-at-tktelekom.pl Sat Mar 29 07:15:38 2014 Return-Path: {drugs_canadian3-at-tktelekom.pl} Received: from 82-160-58-232.tktelekom.pl (82-160-58-232.tktelekom.pl [82.160.58.232]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2TCFYv0004382 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 29 Mar 2014 07:15:35 -0500 Message-Id: {201403291215.s2TCFYv0004382-at-ns.microscopy.com}
X-from: modla-at-dbi.udel.edu ()
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both modla-at-dbi.udel.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
We have a Ditabis Imaging Plate Scanner that we are giving away. The system comes with the lightbox eraser, software CDs, cables, 30 imaging plates, and the cassettes that hold the plates. We also have a PC with the software already installed.
The last time the system was used, it was in good working condition. Since we've upgraded to a new TEM with a CCD camera (it doesn't even have the option for film now), we have not needed to use the Ditabis system.
For those not familiar with the Ditabis, it consists of special imaging plates that you load into the TEM same as film. After you expose the beam to the film, it can be removed and scanned using the Ditabis Micron scanner, and then the high resolution image is available in digital format. The film can then be erased on the lightbox and repeatedly reused.
For anyone interested, please contact me. If you want the system, you will either need to come pick it up or be responsible for the shipping/handling. The scanner itself probably weights a couple hundred pounds. We are located at the University of Delaware in Newark, DE.
Shannon Modla
Research Associate II University of Delaware Delaware Biotechnology Institute BioImaging Center 15 Innovation Way, Suite 117 Newark, DE 19711 modla-at-dbi.udel.edu
Login Host: 128.4.105.38 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Mar 29 08:01:50 2014 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2TD1oJQ004849 18, 28 -- for {microscopy-at-microscopy.com} ; Sat, 29 Mar 2014 08:01:50 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id BA1786DE415 18, 28 -- for {microscopy-at-microscopy.com} ; Sat, 29 Mar 2014 08:01:50 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id IM6wQGO56hl8 for {microscopy-at-microscopy.com} ; 18, 28 -- Sat, 29 Mar 2014 08:01:43 -0500 (CDT) 18, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id 1DAB36DE408 18, 28 -- for {microscopy-at-microscopy.com} ; Sat, 29 Mar 2014 08:01:43 -0500 (CDT) 18, 28 -- Message-ID: {5336C436.2020904-at-microscopy.com} 18, 28 -- Date: Sat, 29 Mar 2014 08:01:42 -0500 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: viaWWW: Free Ditabis Imaging Plate Scanner 18, 28 -- References: {201403281756.s2SHulsO003316-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201403281756.s2SHulsO003316-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201403281756.s2SHulsO003316-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From drugstore-cheapest12-at-suratifarsan.com Sun Mar 30 22:56:54 2014 Return-Path: {drugstore-cheapest12-at-suratifarsan.com} Received: from smtp2.suratifarsan.com ([178.207.57.203]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s2V3up6V000484 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 30 Mar 2014 22:56:52 -0500 Message-Id: {201403310356.s2V3up6V000484-at-ns.microscopy.com}
I'm trying to diagnose a vacuum problem with the Leica S430 that was donated to the Pumping Station: One hackerspace in Chicago. We are, to our knowledge, the only hackerspace in the US with a working SEM, and we are developing programming around making it accessible to the general public.
When the scope arrived, it couldn't achieve vacuum better than 6e-5 torr. Over time, it did substantially better, and, after I changed the roughing pump oil, it could get down, at best, to 1.8e-5 torr. Still, factory specs say it should go to 2e-6, and I'd be much happier if it were in the e-6 range.
However, I'd noticed strange problems where, after a week of running, I'd check it and find that it seemed stuck at a rather awful vacuum of 1.2 to 1.4e-4 torr. The act of venting the chamber and pumping it down again seemed to cure it, and it would pump down rather quickly to 3e-5 torr and slowly get better from there. The oil change didn't solve this. I tried removing the oil mist / odor filter while the vacuum was stalled in the 1.?e-4 torr range to see if it was creating back pressure, but that didn't do much. Since then, I've replaced the oil mist and odor filter elements. This helps somewhat, but not entirely, at removing the hot oil reek when pumping down the chamber from atmospheric pressure.
Things have become substantially worse. Now, pumping down from atmospheric pressure works as expected, but within hours, pressure rises to the 1.2 to 1.4e-4 torr level again. Venting and pumping fixes it temporarily until this happens again in a few hours. Thoughts:
Virtual leak / sample outgassing: I'm doubting this. I keep the scope at vacuum 24x7 and haven't changed out the samples in months. (I'm primarily teaching people to use the scope by looking at samples already prepared. I don't have curriculum together on sample prep, although we do have a sputter coater and critical point dryer.)
Physical leak: That might explain why I'm not getting below 1.8e-5, but I don't see it explaining why the pressure rises so much after a successful pump down. I've also wiped down the chamber and gun O-rings recently with no effect.
Backing pump oil mist / odor filter back pressure: Possible, as I rebuilt the filter recently and could have caused a problem, but this didn't start immediately after I rebuild the filter, and when I tried removing the filter before, it never helped.
Backing pump oil age: I changed it a little over 4 months ago. I suppose this is possible, but I'd not had this problem with this frequency when I was running oil that had been in the pump for a very long time from the former owner.
Air filter dessicant: it's starting to change color in places. I know I should heat it to drive out the moisture. But even if moist air is coming into the chamber when I vent it, I'd think that would slow my pump down time, not cause a normal pump down and reappear as a problem later.
I wouldn't know if this were a problem with the Penning gauge. Usually, after the Penning turns on, I see some sinusoidal oscillation in pressure during initial pump down, but then it damps out and behaves predictably otherwise.
So I'm left thinking this is either a turbomolecular pump problem, or a roughing pump problem. The turbopump does make a soft high pitched whine, which sometimes changes pitch, but I've heard that is normal. The roughing pump does change its sound periodically. Especially when pumping down, it sounds like it is running rough. The scope didn't have a maintenance contract at its former home, so I question how much preventative maintenance was done. If I had to guess, I'd question if the scope needs a rebuild. I've seen kits available. (Edwards E2M12 I think.)
We can't exactly afford thousands of dollars to bring in a SEM tech. Buying a rebuild kit for the roughing pump would be financially doable, but I've heard this is a lot of work (Have any of you rebuilt a multi-stage rotary vane pump? How difficult?), and I'd probably need an offsite area to do the rebuild because our "microscopy lab" is in a corner of a dusty workshop. (Yes, I know this isn't ideal for a SEM....) If possible, I'd really like more of an indication that the roughing pump is the problem before trying this.
Have any of you seen problems like this? Any thoughts on how to proceed?
Thanks, Ryan
==============================Original Headers============================== 15, 16 -- From rdpierce-at-pobox.com Mon Mar 31 23:57:07 2014 15, 16 -- Received: from elna.mackenziegems.com (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) 15, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s314v7Za023685 15, 16 -- for {microscopy-at-microscopy.com} ; Mon, 31 Mar 2014 23:57:07 -0500 15, 16 -- Received: from [192.168.2.102] (unknown [192.168.2.102]) 15, 16 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id 906576E0042 15, 16 -- for {microscopy-at-microscopy.com} ; Mon, 31 Mar 2014 23:57:06 -0500 (CDT) 15, 16 -- Message-ID: {533A4723.1030505-at-pobox.com} 15, 16 -- Date: Mon, 31 Mar 2014 23:57:07 -0500 15, 16 -- From: Ryan Pierce {rdpierce-at-pobox.com} 15, 16 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 15, 16 -- MIME-Version: 1.0 15, 16 -- To: microscopy-at-microscopy.com 15, 16 -- Subject: SEM Vacuum Problems 15, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Announcing a 2014 REU in Materials Science and Engineering "Physical Properties of Materials" to be hosted at Mississippi State University
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Thanks, Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University
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Just a reminder: Wednesday, April 2, 2014, 1:00PM EDT To register: {http://scientific.datacolor.com/resources/events/} http://scientific.datacolor.com/resources/events/
Interpretation and communication are the essence of science. But what happens when what you see in the microscope is not what you see on your monitor? Or your laptop? Or that your colleague sees when he opens that all-important image you sent him? Ironically, these variations in the image are often most pronounced in that good old workhorse, brightfield imaging of stained samples. This webinar will review best practices in brightfield imaging then discuss how Datacolor’s ChromaCal microscope and monitor systems can help you to standardize those images so that what you saw in the microscope is what you get on your screen.
Guest Speaker: Dr. Eduardo Rosa-Molinar Dr. Rosa-Molinar is a Professor in the Department of Biology at the University of Puerto Rico – Rio Piedras. He serves on the FASEB Board of Directors, and has served in numerous positions with The Histochemical Society, the Society for Integrative and Comparative Biology, the Society for Neuroscience, and is currently the Chair of the Andor/Bitplane Advanced Imaging Centers.
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Email: see.wee.chee-at-gmail.com Name: See Wee Chee
Title-Subject: [Filtered] Olympus TEM Cameras
Message: Hello everybody,
The side-mounted camera on our TEM has failed and we are looking for a replacement. We discovered that Olympus is now in market with three side-mounted cameras. The specifications look pretty good but we don't know of anyone that has one in service.
Has anyone here used an Olympus camera on their TEM before? Any feedback on performance, software and support will be greatly appreciated. We are interested in the MegaView G2 and Veleta lines. Thanks!
See Wee
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 1 17:19:37 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s31MJbdX028722 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2014 17:19:37 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 362696E0D43 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2014 17:19:37 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id qk-hZZTVIHda for {microscopy-at-microscopy.com} ; 13, 28 -- Tue, 1 Apr 2014 17:19:31 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 327FB6E0D36 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2014 17:19:31 -0500 (CDT) 13, 28 -- Message-ID: {533B3B73.3030501-at-microscopy.com} 13, 28 -- Date: Tue, 01 Apr 2014 17:19:31 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Olympus TEM Cameras 13, 28 -- References: {201404011837.s31IbLJf025993-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201404011837.s31IbLJf025993-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201404011837.s31IbLJf025993-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Ryan, I doubt that your Rotary pump (RP) is the problem. I'm not familiar with the S430 but have some familiarity with the 1430 and 1430VP. First, check and see if the turbopump is going into a "standby mode" at a reduced RPM. In conjunctin with a leak, this could cause the vacuum deterioration that you are seeing. More likely, the Penning gauge needs to be cleaned. Determine the model and you should be able to find a user's manual on line with instructions on how to clean it.
I have seen systems behave in a somewhat similar manner with oil diffusion pumps due to either a lack of DP oil or because of issues in systems that change the power settings to the DP in different pumping stages. This is not generally the case with TMPs, so clean the Penning Gauge and see what happens.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: rdpierce-at-pobox.com [mailto:rdpierce-at-pobox.com] Sent: Tuesday, April 01, 2014 1:01 AM To: kenconverse-at-qualityimages.biz
I'm trying to diagnose a vacuum problem with the Leica S430 that was donated to the Pumping Station: One hackerspace in Chicago. We are, to our knowledge, the only hackerspace in the US with a working SEM, and we are developing programming around making it accessible to the general public.
When the scope arrived, it couldn't achieve vacuum better than 6e-5 torr. Over time, it did substantially better, and, after I changed the roughing pump oil, it could get down, at best, to 1.8e-5 torr. Still, factory specs say it should go to 2e-6, and I'd be much happier if it were in the e-6 range.
However, I'd noticed strange problems where, after a week of running, I'd check it and find that it seemed stuck at a rather awful vacuum of 1.2 to 1.4e-4 torr. The act of venting the chamber and pumping it down again seemed to cure it, and it would pump down rather quickly to 3e-5 torr and slowly get better from there. The oil change didn't solve this. I tried removing the oil mist / odor filter while the vacuum was stalled in the 1.?e-4 torr range to see if it was creating back pressure, but that didn't do much. Since then, I've replaced the oil mist and odor filter elements. This helps somewhat, but not entirely, at removing the hot oil reek when pumping down the chamber from atmospheric pressure.
Things have become substantially worse. Now, pumping down from atmospheric pressure works as expected, but within hours, pressure rises to the 1.2 to 1.4e-4 torr level again. Venting and pumping fixes it temporarily until this happens again in a few hours. Thoughts:
Virtual leak / sample outgassing: I'm doubting this. I keep the scope at vacuum 24x7 and haven't changed out the samples in months. (I'm primarily teaching people to use the scope by looking at samples already prepared. I don't have curriculum together on sample prep, although we do have a sputter coater and critical point dryer.)
Physical leak: That might explain why I'm not getting below 1.8e-5, but I don't see it explaining why the pressure rises so much after a successful pump down. I've also wiped down the chamber and gun O-rings recently with no effect.
Backing pump oil mist / odor filter back pressure: Possible, as I rebuilt the filter recently and could have caused a problem, but this didn't start immediately after I rebuild the filter, and when I tried removing the filter before, it never helped.
Backing pump oil age: I changed it a little over 4 months ago. I suppose this is possible, but I'd not had this problem with this frequency when I was running oil that had been in the pump for a very long time from the former owner.
Air filter dessicant: it's starting to change color in places. I know I should heat it to drive out the moisture. But even if moist air is coming into the chamber when I vent it, I'd think that would slow my pump down time, not cause a normal pump down and reappear as a problem later.
I wouldn't know if this were a problem with the Penning gauge. Usually, after the Penning turns on, I see some sinusoidal oscillation in pressure during initial pump down, but then it damps out and behaves predictably otherwise.
So I'm left thinking this is either a turbomolecular pump problem, or a roughing pump problem. The turbopump does make a soft high pitched whine, which sometimes changes pitch, but I've heard that is normal. The roughing pump does change its sound periodically. Especially when pumping down, it sounds like it is running rough. The scope didn't have a maintenance contract at its former home, so I question how much preventative maintenance was done. If I had to guess, I'd question if the scope needs a rebuild. I've seen kits available. (Edwards E2M12 I think.)
We can't exactly afford thousands of dollars to bring in a SEM tech. Buying a rebuild kit for the roughing pump would be financially doable, but I've heard this is a lot of work (Have any of you rebuilt a multi-stage rotary vane pump? How difficult?), and I'd probably need an offsite area to do the rebuild because our "microscopy lab" is in a corner of a dusty workshop. (Yes, I know this isn't ideal for a SEM....) If possible, I'd really like more of an indication that the roughing pump is the problem before trying this.
Have any of you seen problems like this? Any thoughts on how to proceed?
Thanks, Ryan
==============================Original Headers============================== 15, 16 -- From rdpierce-at-pobox.com Mon Mar 31 23:57:07 2014 15, 16 -- Received: from elna.mackenziegems.com (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) 15, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s314v7Za023685 15, 16 -- for {microscopy-at-microscopy.com} ; Mon, 31 Mar 2014 23:57:07 -0500 15, 16 -- Received: from [192.168.2.102] (unknown [192.168.2.102]) 15, 16 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id 906576E0042 15, 16 -- for {microscopy-at-microscopy.com} ; Mon, 31 Mar 2014 23:57:06 -0500 (CDT) 15, 16 -- Message-ID: {533A4723.1030505-at-pobox.com} 15, 16 -- Date: Mon, 31 Mar 2014 23:57:07 -0500 15, 16 -- From: Ryan Pierce {rdpierce-at-pobox.com} 15, 16 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 15, 16 -- MIME-Version: 1.0 15, 16 -- To: microscopy-at-microscopy.com 15, 16 -- Subject: SEM Vacuum Problems 15, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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==============================Original Headers============================== 28, 27 -- From kenconverse-at-qualityimages.biz Tue Apr 1 20:13:57 2014 28, 27 -- Received: from cdptpa-oedge-vip.email.rr.com (cdptpa-outbound-snat.email.rr.com [107.14.166.226]) 28, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s321Duf5017106 28, 27 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2014 20:13:56 -0500 28, 27 -- Received: from [108.183.173.180] ([108.183.173.180:2608] helo=Ken) 28, 27 -- by cdptpa-oedge03 (envelope-from {kenconverse-at-qualityimages.biz} ) 28, 27 -- (ecelerity 3.5.0.35861 r(Momo-dev:tip)) with ESMTP 28, 27 -- id F2/98-16704-4546B335; Wed, 02 Apr 2014 01:13:56 +0000 28, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 28, 27 -- To: {rdpierce-at-pobox.com} , "MSA Listserver" {microscopy-at-microscopy.com} 28, 27 -- Subject: RE: [Microscopy] SEM Vacuum Problems 28, 27 -- Date: Tue, 1 Apr 2014 21:14:26 -0400 28, 27 -- Message-ID: {DD64DE01CEAA446995DA75A455DB4C6A-at-Ken} 28, 27 -- MIME-Version: 1.0 28, 27 -- Content-Type: text/plain; 28, 27 -- charset="us-ascii" 28, 27 -- X-Priority: 3 (Normal) 28, 27 -- X-MSMail-Priority: Normal 28, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 28, 27 -- thread-index: Ac9NZ2ZIRfwIiJWETGaDy6KsXI6RnwAp9Iwg 28, 27 -- Importance: Normal 28, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 28, 27 -- In-Reply-To: {201404010500.s3150XpS026767-at-ns.microscopy.com} 28, 27 -- X-RR-Connecting-IP: 107.14.168.142:25 28, 27 -- X-Cloudmark-Score: 0 28, 27 -- Content-Transfer-Encoding: 8bit 28, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s321Duf5017106 ==============================End of - Headers==============================
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Email: n00183308-at-ospreys.unf.edu Name: Jason Saredy
I have circuit diagrams (for each component) and part list from a 35CF I was repairing years ago. While the department ended up trashing the equipment, I still have diagrams. I have no idea whether JEOL offers these in electronic formats, but I'd rather see the paper outside of a garbage heap. Willing to eat mailing costs within the US, beautiful drawings in general and decent condition.
-Jason Saredy N00183308-at-ospreys.unf.edu
PS for some reason the server keeps rejecting my email, school transferred services and domain names for our emails... so submitting from online form.
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Message: What is the proper way to change the oil in the diffusion pump on the Philips CM10/12? The manual does not say how to release the vacuum in the pump. I removed the dipstick on the pump and noticed that while the pressure was equalizing, most of the oil from the pump got sucked into the tubes going to the buffer tank and the one to the rough pump. I know now that there was too much oil in it, but is that the proper way to release the vacuum?
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Email: pinard-at-gfe.rwth-aachen.de Name: Philippe Pinard
Organization: RWTH Aachen University
Title-Subject: [Filtered] Monte Carlo Simulations for Electron Microscopy in Aachen (Germany)
Message: Dear fellow microscopists,
The Central Facility for Electron Microscopy of the RWTH Aachen University in collaboration with the European Microbeam Analysis Society would like to invite you to the "Monte Carlo Simulations for Electron Microscopy" workshop, that will be held at the RWTH Aachen University (Germany) from June 10th to 12th, 2014.
The aim of this workshop is to provide electron microscopists in-depth knowledge of Monte Carlo simulation methods to assist them in their daily scientific work. Lectures from leading Monte Carlo experts will cover both theoretical and practical aspects of current Monte Carlo programs. Tutorials and live demonstrations will highlight common applications of Monte Carlo simulations to the field of electron microscopy.
The registration for the workshop costs 150 euros for EMAS members or RWTH personnel and 200 euros for other participants. The course is designed for any scientist (students, technicians, researchers) having basic knowledge in EPMA, SEM and TEM.
For the full program, registration form and any other information about the workshop, please visit our website: http://www.gfe.rwth-aachen.de/montecarlo
Sincerely yours, Philippe Pinard and Silvia Richter
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From affordable-meds14-at-telecom.kz Wed Apr 2 09:59:15 2014 Return-Path: {affordable-meds14-at-telecom.kz} Received: from 2.135.92.233.megaline.telecom.kz (2.135.92.233.megaline.telecom.kz [2.135.92.233] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s32ExB4l005618 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 2 Apr 2014 09:59:12 -0500 Message-Id: {201404021459.s32ExB4l005618-at-ns.microscopy.com}
Hello Josh, Bellow I add a copy of two paragraphs from the original 30 years old manual for CM10/12. You have to follow all the steps.
---------------------------- 6. 2. 1. ADMITTING AIR INTO THE PUMPING SYSTEM Before air is let into the pumping system the OD pump should be allowed to cool down for at least 1 hour after being switched off (either by switching off the microscope or by removal of the OD pump fuse). Air should first be admitted into the projection chamber (operating the "cam air" softkey), then let air into the buffer tank and the diffusion pump by very slowly and carefully loosening the dipstick 216 to 219, Fig. 820.
Air is let into the pre—vacuum lines by opening valve V1 using the service key and the manual page. Air can also be admitted into the pre- vacuum lines by removing Pirani P2. (Here should be P1, I think.) ----------------------------
Best regards from Prague Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
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Title-Subject: [Filtered] Precise measurement of particles from Rotary shadowing of freeze fracture replicas
Message: I would like to measure the diameter of certain IMPs on protoplasmic fracture face (PF) of a plant cell plasma membrane. I am fracturing at -160 degree C under a vacuum less than 10 to -7 mbar in a Cressington 308 freeze fracture machine. The platinum-carbon (Pt/C) is depositing from a nominal 60 degree angle (the angle is relative to the specimen stage), and specimen is rotating at maximum speed (the revolution per min is unknown but can be calculated). The rotary shadowing of Pt/C is followed by carbon shadowing to stabilize the replica. The deposited Pt/C is measured by a Quartz crystal and displayed on a “thickness monitor”. I would like to know you answers or comments about following questions:
1-With rotary shadowing what formula is used to calculate shadowed metal on a sphere particle? 2-How the angle that Pt/C is deposited from (e.g. 45 or 60 degree) incorporated to the formula? 3-Specimen rotation is used to uniform the shadowing on particle surface, however, dose it need to be incorporated in the formula? 4-What is the formula for a unidirectional shadowing? Is it subtracting two times of the deposited metal (read by Quartz crystal) from the diameter of shadowed particle?
Thanks,
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From mlequily-at-vnpt-hanoi.com.vn Wed Apr 2 21:47:59 2014 Return-Path: {mlequily-at-vnpt-hanoi.com.vn} Received: from static.vnpt-hanoi.com.vn (static.vnpt-hanoi.com.vn [113.190.242.243] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s332lvhg026914 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 2 Apr 2014 21:47:58 -0500 Message-Id: {201404030247.s332lvhg026914-at-ns.microscopy.com}
Hi!
We have a Megaview II camera mounted on a Tecnai G20 instrument and are happy with it. Before SIS was bought by Olympus we got really good support. This is not a critic to Olympus, it is just that since then I never needed help anymore ;-) Actually we never got any problem, I just needed help for optimization and the support was both friendly and efficient. I find the original software better than the soft from FEI to control the camera. I have no personnal interest but I can recommend it.
Stephane
________________________________ X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: nizets2-at-yahoo.com Sent: Wednesday, April 2, 2014 12:25 AM
X-from: see.wee.chee-at-gmail.com ()
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Email: see.wee.chee-at-gmail.com Name: See Wee Chee
Title-Subject: [Filtered] Olympus TEM Cameras
Message: Hello everybody,
The side-mounted camera on our TEM has failed and we are looking for a replacement. We discovered that Olympus is now in market with three side-mounted cameras. The specifications look pretty good but we don't know of anyone that has one in service.
Has anyone here used an Olympus camera on their TEM before? Any feedback on performance, software and support will be greatly appreciated. We are interested in the MegaView G2 and Veleta lines. Thanks!
See Wee
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 1 17:19:37 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s31MJbdX028722 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2014 17:19:37 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 362696E0D43 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2014 17:19:37 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id qk-hZZTVIHda for {microscopy-at-microscopy.com} ; 13, 28 -- Tue, 1 Apr 2014 17:19:31 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 327FB6E0D36 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2014 17:19:31 -0500 (CDT) 13, 28 -- Message-ID: {533B3B73.3030501-at-microscopy.com} 13, 28 -- Date: Tue, 01 Apr 2014 17:19:31 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Olympus TEM Cameras 13, 28 -- References: {201404011837.s31IbLJf025993-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201404011837.s31IbLJf025993-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201404011837.s31IbLJf025993-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This is my first post to the list and while I have 40 years of microscopy experience I am new to the list so if my post is not in alignment with the rules I am very receptive to input so that I can be a productive list member.
Although it is not a TEM camera, we just put in a new Olympus research grade compound scope and elected to buy their higher end DP80 camera which was more costly than other options from third parties. That said, I have no regrets and the image quality is phenomenal. I am really glad that I elected to purchase it. This is a new system so I have no input on lifetime but I can say that I have never had such excellent images from a microscope before. Their service and support have been very good and the overall experience was very good. I would not hesitate to purchase another scope from Olympus and in fact we are getting ready to. If their TEM camera performs for you like the DP80 does for us I think you would be very happy. Hopefully, someone on the list with direct experience can provide feedback on the actual model you are looking at. You should also be able to work with one at a demo center which I would encourage you to do if you are not sure.
Sincerely,
James Neal-Kababick Director Flora Research Laboratories An FDA and DEA Registered & Inspected Laboratory Fellow AOAC International Adjunct Faculty Bastyr University Botanical Medicine Department 1-541-472-0980 phone jimk-at-floraresearch.com www.floraresearch.com
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==============================Original Headers============================== 10, 28 -- From jimk-at-floraresearch.com Thu Apr 3 12:54:17 2014 10, 28 -- Received: from xsmtp08.mail2web.com (xsmtp08.mail2web.com [168.144.250.229]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s33HsHq9024585 10, 28 -- for {Microscopy-at-Microscopy.com} ; Thu, 3 Apr 2014 12:54:17 -0500 10, 28 -- Received: from [10.5.31.8] (helo=hub02.ad2.softcom.biz) 10, 28 -- by xsmtp08.mail2web.com with esmtps (TLS-1.0:RSA_ARCFOUR_MD5:16) 10, 28 -- (Exim 4.63) 10, 28 -- (envelope-from {jimk-at-floraresearch.com} ) 10, 28 -- id 1WVlpi-0001eD-7s 10, 28 -- for Microscopy-at-Microscopy.com; Thu, 03 Apr 2014 13:54:16 -0400 10, 28 -- Received: from MBX74.ad2.softcom.biz ([10.5.13.37]) by hub02.ad2.softcom.biz 10, 28 -- ([10.5.31.8]) with mapi; Thu, 3 Apr 2014 13:53:45 -0400 10, 28 -- From: James Neal-Kababick {jimk-at-floraresearch.com} 10, 28 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 10, 28 -- Date: Thu, 3 Apr 2014 13:53:47 -0400 10, 28 -- Thread-Topic: Re: Olympus TEM Cameras 10, 28 -- Thread-Index: Ac9PZam8y/3DzyAXQ/yrfR6GOTYt5w== 10, 28 -- Message-ID: {143B8CF2094404458B4AA679F5A9877B6D9A4A90E0-at-MBX74.ad2.softcom.biz} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- Subject: Re: Olympus TEM Cameras 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s33HsHq9024585 ==============================End of - Headers==============================
From meds-affordable9-at-kes.ru Thu Apr 3 14:24:33 2014 Return-Path: {meds-affordable9-at-kes.ru} Received: from 31.181.132.1.kes.ru ([31.181.210.214]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s33JOU64012161 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 3 Apr 2014 14:24:31 -0500 Message-Id: {201404031924.s33JOU64012161-at-ns.microscopy.com}
Greetings All,
I was hoping to get any information regarding immuno-gold labeling workshops/courses being offered this year in The U.S. I currently have quite a bit of experience in conventional biological TEM work but was hoping to expand my capacity to include immuno gold labeling. I have no experience with this technique and was hoping some sort of course or workshop might exist.
Kind Regards,
David Cugier, Associate Scientist II Investigative Tox/Path-Preclinical Safety Division Dept. R45M AbbVie, Inc. 1 North Waukegan Road North Chicago, Illinois 60064
From pharmacy_discounted13-at-schaperpaint.com Fri Apr 4 21:17:49 2014 Return-Path: {pharmacy_discounted13-at-schaperpaint.com} Received: from mail4.schaperpaint.com ([91.235.213.71]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s352HmHI002241 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 4 Apr 2014 21:17:48 -0500 Message-Id: {201404050217.s352HmHI002241-at-ns.microscopy.com}
X-from: analytic-at-rawbw.com ()
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Organization: Northern California Society of Microscopy
Title-Subject: [Filtered] Spring Meeting
Message: Subject: [Filtered] Northern California Society for Microscopy Spring Meeting April 17th
Northern California Society for Microscopy Spring Meeting
Thursday, April 17, 2014 6:00 PM to 9:00 PM Please RSVP to info-at-ncsmicroscopy.org
Scotts of Palo Alto #1 Town and Country Village Palo Alto, California http://scottsseafoodpa.com/
Featured Speakers Are:
Kent McDonald, PhD, Electron Microscopy Lab, UC Berkeley
Rapid Specimen Processing Methods for Cryofixed Samples
New methods for freeze substitution have been developed that require only basic laboratory tools. Excellent freeze substitution results can be obtained in as little as 90 min for cells of small volume. Rapid epoxy resin infiltration can be achieved by centrifugation followed by polymerization at 100 C for 1.5–2 h. Total processing time from freezing to blocks ready to section is about 6 h. Comparative results will be presented.
Ryan McGorty, PhD, UC San Francisco, School of Pharmacy
Three-dimensional super-resolution imaging of tissue samples
Single-molecule localization microscopy methods are often used to study samples at or very near the coverslip surface with a spatial resolution of around 20nm. However, a number of challenges emerge when trying to image even a few microns past the coverslip surface, particularly when trying to accurately localize molecules in 3D. After first explaining the principle of the super-resolution microscopy technique we use, I will discuss multiple methods we have recently developed that allow us to image deeper into tissue samples.
No Cost to Current Members. $25 for Non-Members.
Bring your check for 2014 dues and enjoy a 3-course meal and great speakers. Annual dues for regular members are *$40*; students, *$20*; and a corporate membership(which includes one person) is *$100, *payable to *NCSM*
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==============================Original Headers============================== 26, 29 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 7 07:30:38 2014 26, 29 -- Received: from znl.com ([206.69.208.20]) 26, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s37CUcvv030458 26, 29 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 07:30:38 -0500 26, 29 -- Received: from localhost (localhost [127.0.0.1]) 26, 29 -- by znl.com (Postfix) with ESMTP id 0C9116E50E4 26, 29 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 07:30:38 -0500 (CDT) 26, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 26, 29 -- Received: from znl.com ([127.0.0.1]) 26, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 26, 29 -- with ESMTP id ubc8cAGNMiP5 for {microscopy-at-microscopy.com} ; 26, 29 -- Mon, 7 Apr 2014 07:30:29 -0500 (CDT) 26, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 26, 29 -- by znl.com (Postfix) with ESMTPA id EFB006E50D2 26, 29 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 07:30:28 -0500 (CDT) 26, 29 -- Message-ID: {53429A64.6030902-at-microscopy.com} 26, 29 -- Date: Mon, 07 Apr 2014 07:30:28 -0500 26, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 26, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 26, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 26, 29 -- MIME-Version: 1.0 26, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 26, 29 -- Subject: viaWWW:Northern California Society for Microscopy Spring Meeting 26, 29 -- ,April 17th 26, 29 -- References: {201404041704.s34H4gQJ020292-at-ns.microscopy.com} 26, 29 -- In-Reply-To: {201404041704.s34H4gQJ020292-at-ns.microscopy.com} 26, 29 -- X-Forwarded-Message-Id: {201404041704.s34H4gQJ020292-at-ns.microscopy.com} 26, 29 -- Content-Type: text/plain; charset=UTF-8; format=flowed 26, 29 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Message: Does anyone have a service key for the philips CM10/12 or know how to acquire one?
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 7 07:31:18 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s37CVIgE031066 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 07:31:18 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 567F96E50F7 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 07:31:18 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id 2HJ-Xwq+Ddif for {microscopy-at-microscopy.com} ; 13, 28 -- Mon, 7 Apr 2014 07:31:13 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 0356F6E50E5 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 07:31:13 -0500 (CDT) 13, 28 -- Message-ID: {53429A90.4070702-at-microscopy.com} 13, 28 -- Date: Mon, 07 Apr 2014 07:31:12 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:CM10 service key 13, 28 -- References: {201404041815.s34IFdkY021126-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201404041815.s34IFdkY021126-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201404041815.s34IFdkY021126-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: hipps-at-wsu.edu Name: K W Hipps
Organization: Washington State University
Title-Subject: [Filtered] Want to buy a Pico STM
Message: Does anyone have an Agilent or Molecular Imaging Pico STM they want to sell? If so, I have $$$
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 7 07:31:57 2014 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s37CVvsE032338 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 07:31:57 -0500 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id C3A936E5108 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 07:31:57 -0500 (CDT) 15, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id BdcpBwcEGVwx for {microscopy-at-microscopy.com} ; 15, 28 -- Mon, 7 Apr 2014 07:31:52 -0500 (CDT) 15, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 7E26F6E50FB 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 07:31:52 -0500 (CDT) 15, 28 -- Message-ID: {53429AB8.6050403-at-microscopy.com} 15, 28 -- Date: Mon, 07 Apr 2014 07:31:52 -0500 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:Want to buy a Pico STM 15, 28 -- References: {201404070006.s3706wgp012816-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201404070006.s3706wgp012816-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201404070006.s3706wgp012816-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
the 4th UniBe Practical Course on Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS, a.k.a cryoEM of frozen-hydrated sections)
11-13 August 2014 / 14-16 August 2014
Organiser : Benoît Zuber Instructors: Benoît Zuber, Daniel Studer, Ioan Iacovache
The objective of the course is to teach the participants the practical skills necessary to successfully apply CEMOVIS in their laboratories. Essential background theory of CEMOVIS will be given and most of the time will be spent practicing high-pressure freezing, cryo-sectioning, and low-dose TEM imaging. 1 high-pressure freezing machine, 3 state-of-the-art cryo-ultramicrotomes and 1 cryo-electron microscope will be dedicated to the course. Of special interest, participants will learn to use the new tools that we developed to facilitate cryosectioning (Studer et al 2014 J Struct Biol 185(1):125-8). The 3-day course is given twice, each time with a maximum of 4 participants so that they can be actively practicing at any time.
The course is intended for scientists whose research projects will benefit from the use of CEMOVIS. Experience in either cryo-electron microscopy or ultramicrotomy of resin-embedded specimens is a prerequisite.
Information and registration:
http://www.ana.unibe.ch/events/cemovis/index.html
Registration deadline: 15.04.2014
Yours faithfully
Benoît Zuber
Prof. Benoît Zuber Institute of Anatomy University of Bern Baltzerstrasse 2 CH-3000 Bern 9 Switzerland Tel. +41 31 631 84 40 benoit.zuber-at-ana.unibe.ch http://www.ana.unibe.ch/~exmo/
==============================Original Headers============================== 16, 29 -- From benoit.zuber.work-at-gmail.com Tue Mar 4 03:36:18 2014 16, 29 -- Received: from mail-ob0-f193.google.com (mail-ob0-f193.google.com [209.85.214.193]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s249aIWs020921 16, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2014 03:36:18 -0600 16, 29 -- Received: by mail-ob0-f193.google.com with SMTP id gq1so2825936obb.8 16, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 04 Mar 2014 01:36:18 -0800 (PST) 16, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 29 -- d=gmail.com; s=20120113; 16, 29 -- h=mime-version:date:message-id:subject:from:to:content-type 16, 29 -- :content-transfer-encoding; 16, 29 -- bh=lKWk/ACoLkqIi4/lq3jocmTMNTEKRyWsIpH4aGUgEDc=; 16, 29 -- b=VlLJd0vQh98Qse/9mJ0pJ0f2OYivEGu38uGznV2kAQUYsOqJLggfhOoV7fxC4Nt/q6 16, 29 -- jkGC+nhqSFnUGomdGBUZ4khFHA8DCpmykCmVN0/UNkOfSslDGrBJV6EqgFAzesj8QYN6 16, 29 -- zgM1rq1/L++8DUr1FQXlmcCxCMGD7LC14qEJTWDWJPBuegDjRSJtO1AijblLo+vQu0/1 16, 29 -- Q7ZgbJQm77V017HhSrr3ysEoip/wvp0G/PbH6hhgHPakqMxd7eHPAY+vXGlIlPAhTv2Z 16, 29 -- 0Yx1kTDUFbsCLsvUnc82eBZe8c0tWlNdu8Yj0khuUX15BfjQR4dHi6R4bdU2nYhMwAHX 16, 29 -- QUzg== 16, 29 -- MIME-Version: 1.0 16, 29 -- X-Received: by 10.182.102.134 with SMTP id fo6mr31490668obb.10.1393925778214; 16, 29 -- Tue, 04 Mar 2014 01:36:18 -0800 (PST) 16, 29 -- Received: by 10.76.2.73 with HTTP; Tue, 4 Mar 2014 01:36:18 -0800 (PST) 16, 29 -- Date: Tue, 4 Mar 2014 10:36:18 +0100 16, 29 -- Message-ID: {CAF5rG8TmyMNc=wpQKVR+wnG5o+Cw7LP732R+e08K4jw6V4cOLA-at-mail.gmail.com} 16, 29 -- Subject: CEMOVIS / frozen-hydrated sections course announcement 16, 29 -- From: =?ISO-8859-1?Q?Beno=EEt_Zuber?= {benoit.zuber.work-at-gmail.com} 16, 29 -- To: Microscopy-at-microscopy.com 16, 29 -- Content-Type: text/plain; charset=ISO-8859-1 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s249aIWs020921 ==============================End of - Headers==============================
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REMINDER:
Dear Colleagues, I appreciate if there are some dermatologist and skin researchers using the Microscopy Listserver, who might find this information useful:
41st Annual Meeting of the SCUR (Society for Cutaneous Ultrastructure Research ROME,ITALY; 2014) June 5-7,2014
NEW: 2nd Announcement
"VILLA MONDRAGONE", Monte Porzio Catone (RM, ITALY) JUNE 5-7, 2014 GENERAL TOPIC: "SKIN AGEING and CANCER: From ULTRASTRUCTURE to CLINIC" INFORMATION (Flyer, all forms) -at- http://www.scur.org/content/e1174/e1181/e1182/index_ger.html
EXTENDED DEADLINE for ABSTRACT SUBMISSION: Thursday, 10th April 2014
Call for papers (in dermatology, dermatopathology, skin research, presenting results using all ancient and modern imaging techniques)
Registration and Accommodation information available.
The deadline to submit titles and abstracts is April 10th, 2014. (late entries will be accepted on demand and need to be indicated by a mail to the Local Organizers at costanza-at-med.uniroma2.it; anapat-at-uniroma2.it; a.orlandi-at-uniroma2.it )
For more information, visit our website at: http://www.scur.org/content/e1174/e1181/e1182/index_ger.html or www.scur.org
Contact us (in case of requests for special information) by email also to: W.Muss-at-salk.at
We look forward to seeing you at the 41st Ann. Meeting of the SCUR in Monte Porzio Catone (RM), 20 km southeast of ROME city center (FRASCATI, near airport CIAMPINO or FIUMICINO), a marvelous place to stay!
NEW: Abstracts of 40th Annual Meeting of the SCUR = 6th Joint Meeting of SCUR with SSSR, the Society for Skin Structure Research (Japan)in SALZBURG, AUSTRIA, May 2013 -at- http://www.nature.com/jid/journal/v134/n4/index.html#Abstracts
Best regards, Wolfgang Muss, PhD ........................................ SCUR Secretary Wolfgang MUSS EM-Lab, Univ. Inst. Pathology, SALK-LK (Gen. Hospital) and PMU (Private Paracelsus Medical University) Salzburg A-5020 SALZBURG-AUSTRIA
==============================Original Headers============================== 23, 40 -- From W.Muss-at-salk.at Mon Apr 7 10:20:00 2014 23, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 23, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s37FK0Ut011681 23, 40 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 10:20:00 -0500 23, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 23, 40 -- by hermes.salk.at (Postfix) with ESMTP id EF3D8646B35 23, 40 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 17:19:58 +0200 (CEST) 23, 40 -- X-Scanned-By: SALK-Content-Filter 23, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 23, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 23, 40 -- with ESMTP id yQFkZHgontn4 for {microscopy-at-microscopy.com} ; 23, 40 -- Mon, 7 Apr 2014 17:19:58 +0200 (CEST) 23, 40 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 23, 40 -- by hermes.salk.at (Postfix) with ESMTP id CC23E646B37 23, 40 -- for {microscopy-at-microscopy.com} ; Mon, 7 Apr 2014 17:19:58 +0200 (CEST) 23, 40 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 23, 40 -- by N2EX199.lks.local with ESMTP; 07 Apr 2014 17:24:35 +0200 23, 40 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 23, 40 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 23, 40 -- 14.02.0387.000; Mon, 7 Apr 2014 17:19:58 +0200 23, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 23, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 23, 40 -- Subject: Reminder: 41st Ann. Meetg of the SCUR (Society for Cutaneous 23, 40 -- Ultrastructure Research, ROME, ITALY; June 5-7, 2014) Deadline for abstract 23, 40 -- submission extended to April 10th 23, 40 -- Thread-Topic: Reminder: 41st Ann. Meetg of the SCUR (Society for Cutaneous 23, 40 -- Ultrastructure Research, ROME, ITALY; June 5-7, 2014) Deadline for abstract 23, 40 -- submission extended to April 10th 23, 40 -- Thread-Index: Ac9SdNYsrhP5k6mETO+zP/znQZ4U2A== 23, 40 -- Date: Mon, 7 Apr 2014 15:19:58 +0000 23, 40 -- Message-ID: {3D22E6EE65F39E448DCE4162815376711C6FD446-at-N2DT131.lksdom21.lks.local} 23, 40 -- Accept-Language: de-DE, en-US 23, 40 -- Content-Language: de-DE 23, 40 -- X-MS-Has-Attach: 23, 40 -- X-MS-TNEF-Correlator: 23, 40 -- x-originating-ip: [192.168.42.2] 23, 40 -- Content-Type: text/plain; charset="iso-8859-1" 23, 40 -- MIME-Version: 1.0 23, 40 -- Content-Transfer-Encoding: 8bit 23, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s37FK0Ut011681 ==============================End of - Headers==============================
From lseprupy-at-avangarddsl.ru Mon Apr 7 13:55:21 2014 Return-Path: {lseprupy-at-avangarddsl.ru} Received: from ip.178-70-60-43.avangarddsl.ru (ip.178-70-60-43.avangarddsl.ru [178.70.60.43] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s37ItJQD000449 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 7 Apr 2014 13:55:20 -0500 Message-Id: {201404071855.s37ItJQD000449-at-ns.microscopy.com}
Greetings
This is a long shot, and may be of interest only to microscopists in the SF bay area.
Our EM technology training program is looking for individuals to serve on our advisory committee. We are required to have outside reviewers help us chart the direction and future of our program, we do this using an advisory committee.
We invite anyone interested in helping us do the right thing to join the committee. We have about two in person meetings on campus in Stockton each year, so the ability to be on campus for these meetings is a plus. However, even distant advisors can help us via email, skype, etc. We are particularly looking for a someone familiar with biological techniques, but everyone is welcome.
Please let me know if you are interested or need more information.
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA-ARS-ERRC
Title-Subject: [Filtered] AFM classes this year?
Message: Hiya, anyone know of any AFM classes this year?
I saw the one in Ohio in July, but was looking for other dates.
thanks
Joe
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Email: veilcs-at-earthlink.net Name: Ron Veil
Organization: Veil Electron Instrument Lab Customer Services
Title-Subject: [Filtered] RE: (Philips) CM10 service key
Message: the service key was given out to field service technicians when the CM was in production in the '80s and '90s. The techs usually got these when they went to the factory training schools. The service key had information burned onto an ePROM that one could use to manipulate the 'scope "manually"; eg: to operate the vacuum sequence to check for leaks or proper operation. This is "old technology" (~30 yrs) and both the ePROM series AND ePROM 'burners' are basically impossible to find.
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Email: js51-at-princeton.edu Name: John Schreiber
Organization: Princeton Univ
Title-Subject: [Filtered] SIS MegaViewII camera
Message: Does anyone have a old SIS MegaviewII camera that you would like to get rid of. I am looking for parts to get the one we have working.
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From express-drugstore8-at-rr.com Tue Apr 8 06:52:05 2014 Return-Path: {express-drugstore8-at-rr.com} Received: from cpe-066-057-220-045.sc.res.rr.com (cpe-066-057-220-045.sc.res.rr.com [66.57.220.45]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s38Bq4hl024719 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 8 Apr 2014 06:52:05 -0500 Message-Id: {201404081152.s38Bq4hl024719-at-ns.microscopy.com}
X-from: lucy.avati-at-sydney.edu.au ()
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Email: lucy.avati-at-sydney.edu.au Name: Lucy Avati
Organization: University of Sydney
Title-Subject: [Filtered] Job vacancy: Light Microscopist / Senior Light Microscopist at the University of Sydney
Message: Dear all,
The University of Sydney is currently seeking a Light Microscopist / Senior Light Microscopist. For more information and to apply, visit sydney.edu.au/recruitment and search by the reference number 2440/1113A.
LIGHT MICROSCOPIST / SENIOR LIGHT MICROSCOPIST SYDNEY MICROSCOPY & MICROANALYSIS DEPUTY VICE-CHANCELLOR RESEARCH REFERENCE NO. 2440/1113A • Expertise in light/optical microscopy essential • Full time fixed term to 31 December 2016, remuneration package: up to $95K p.a. which includes leave loading and up to 17% super
Sydney Microscopy & Microanalysis (SMM) aims to be a world-leading facility for modern microscopy and microanalysis, offering premier instruments, services and training to researchers from across Sydney and around Australia, and providing strong leadership in the national and international characterisation communities.
An exciting opportunity now exists to join SMM in the premier light optical microscopy position of 2014. In this role, you will work within SMMÂ’s existing facility and within the newly established Cellular Imaging Facility at the UniversityÂ’s Charles Perkins Centre (CPC). The CPC is a new, state-of-the-art, world class research and education precinct that is addressing the global epidemic of obesity, diabetes and cardiovascular disease. The CPC will facilitate world class research by translating knowledge into practical solutions, developing innovative, cross-disciplinary teaching programs to inspire the next generation of researchers, and forging new research partnerships within and beyond the University to enable the Centre to deliver unique insights and results.
Reporting to the Directors of SMM and the Laboratory Manager, you will provide high level support to users across SMMÂ’s light optical facilities, focussing primarily on specialist techniques in microscopy, microanalysis, and interpretation and analysis of images and data, to ensure these facilities provide effective, high-quality outcomes for users.
In this role you will: • maintain the light/optical facilities in consultation with the Light Microscopy co-ordinator and extended team, the Laboratory Manager and Directors • work with the Light Optical team to plan, prepare and deliver the training program for user groups in light/optical section • instruct, demonstrate, train and assist users in their research and develop a user portfolio • ensure proper and safe operation of light/optical microscopes and related equipment and ensure implementation of WHS rules and regulations • develop and maintain good relations with relevant instrument and equipment vendors.
To qualify for this role, you will demonstrate: • extensive training, experience and expertise in light-optical microscopy and associated specimen preparation techniques and data analysis • skills in advanced light/optical techniques, including, FRET, FLIM, FRAP, ratiometric and live-cell, along with the associated analysis techniques • experience in the servicing and maintenance of microscopes and demonstrated ability to run and maintain sophisticated scientific instrumentation • experience in the day-to-day running/operation issues of a multi-user laboratory and experience in WHS and risk assessment preparation and legislative requirements
The successful candidate must possess a bachelorÂ’s degree in science or engineering. A postgraduate degree in this field will be highly regarded.
Remuneration package for HEO 7 appointment: $95,168p.a. (which includes a base salary of $80,418 p.a., leave loading and up to 17% employerÂ’s contribution to superannuation).
Remuneration package for HEO 6 appointment: $86,658p.a. (which includes a base salary of $73,227 pa., leave loading and up to 17% employerÂ’s contribution to superannuation).
Specific enquiries about the role can be directed to Eleanor Kable, Laboratory Manager, on +61 2 9351 7566.
General recruitment enquiries can be directed to Melanie Leshetz or Cherie Goodwin on +61 2 8627 1247.
CLOSING DATE: 5pm 2 May 2014
The University is an equal opportunity employer committed to equity, diversity and social inclusion. Applications from equity target groups and women are encouraged. The University of Sydney has also established a scheme to increase the number of Aboriginal and Torres Strait Islander staff employed across the institution. Applications from people of Aboriginal and Torres Strait Islander descent are encouraged.
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Hi all,
I am searching for a method to preferentially stain lignin for SEM or TEM. The object is to be able to determine if the lignin is present in nano-size wood particles. Safranin can be used to stain lignin for LM but does not contain a heavy element that will scatter electrons so that it can be identified by EM.
Any ideas would be appreciated.
Debby
Debra Sherman DS imaging LLC Purdue Technology Center 1281 Win Hentschel Blvd West Lafayette, IN 47906 E-mail: debby.sherman-at-dsimagingllc.com www.dsimagingllc.com Mobile: 765-418-8540
==============================Original Headers============================== 13, 31 -- From dsherman-at-purdue.edu Wed Apr 9 20:36:42 2014 13, 31 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3A1afBg017682 13, 31 -- for {microscopy-at-microscopy.com} ; Wed, 9 Apr 2014 20:36:42 -0500 13, 31 -- Received: from WPVEXCHUB02.purdue.lcl (wpvexchub02.itap.purdue.edu [172.30.136.64]) 13, 31 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s3A1afhf025831 13, 31 -- for {microscopy-at-microscopy.com} ; Wed, 9 Apr 2014 21:36:41 -0400 13, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([169.254.2.193]) by 13, 31 -- WPVEXCHUB02.purdue.lcl ([172.30.136.64]) with mapi id 14.03.0174.001; Wed, 9 13, 31 -- Apr 2014 21:36:41 -0400 13, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 13, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 13, 31 -- Subject: lignin staining for TEM or SEM 13, 31 -- Thread-Topic: lignin staining for TEM or SEM 13, 31 -- Thread-Index: AQHPVF1SFqdF+VAZwkySmcJF426DhQ== 13, 31 -- Date: Thu, 10 Apr 2014 01:36:40 +0000 13, 31 -- Message-ID: {CF6B6E38.1C8B5%dsherman-at-purdue.edu} 13, 31 -- Accept-Language: en-US 13, 31 -- Content-Language: en-US 13, 31 -- X-MS-Has-Attach: 13, 31 -- X-MS-TNEF-Correlator: 13, 31 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 13, 31 -- x-originating-ip: [24.14.170.252] 13, 31 -- Content-Type: text/plain; charset="us-ascii" 13, 31 -- Content-ID: {2124549E5A878A49AF44C34CF8F0D41F-at-exchange.purdue.edu} 13, 31 -- MIME-Version: 1.0 13, 31 -- X-PMX-Version: 6.0.2.2308539 13, 31 -- X-PerlMx-URL-Scanned: Yes 13, 31 -- X-PerlMx-Virus-Scanned: Yes 13, 31 -- Content-Transfer-Encoding: 8bit 13, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3A1afBg017682 ==============================End of - Headers==============================
If the lignin is at least partly exposed on the particle surface, you could use antibodies against lignin. With proper controls that will be a highly specific identification. See e.g. Visualizing Lignin Coalescence and Migration Through Maize Cell Walls Following Thermochemical Pretreatment, Bryon S. Donohoe, Stephen R. Decker, Melvin P. Tucker, Michael E. Himmel, Todd B. Vinzant Biotechnology and Bioengineering, Vol. 101, No. 5, December 1, 2008
It does not have to be gold-labelled, but could be something like a solid phase immunoassay type of protocol: antibodies against lignin bound to a solid fase, which could be a film on a grid. Then incubate with the lignin suspension and see if you have specific binding on the film.
Will be happy to help. if required, with more details.
Cheers,
Jan
Jan Leunissen Aurion i: www.aurion.nl Dept Geology University of Otago Dunedin, New Zealand
On 10/04/2014, at 1:37 pm, dsherman-at-purdue.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } } I am searching for a method to preferentially stain lignin for SEM or TEM. } The object is to be able to determine if the lignin is present in } nano-size wood particles. Safranin can be used to stain lignin for LM but } does not contain a heavy element that will scatter electrons so that it } can be identified by EM. } } Any ideas would be appreciated. } } Debby } } } Debra Sherman } DS imaging LLC } Purdue Technology Center } 1281 Win Hentschel Blvd } West Lafayette, IN 47906 } E-mail: debby.sherman-at-dsimagingllc.com } www.dsimagingllc.com } Mobile: 765-418-8540 } } } } } } } } } ==============================Original Headers============================== } 13, 31 -- From dsherman-at-purdue.edu Wed Apr 9 20:36:42 2014 } 13, 31 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) } 13, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3A1afBg017682 } 13, 31 -- for {microscopy-at-microscopy.com} ; Wed, 9 Apr 2014 20:36:42 -0500 } 13, 31 -- Received: from WPVEXCHUB02.purdue.lcl (wpvexchub02.itap.purdue.edu [172.30.136.64]) } 13, 31 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s3A1afhf025831 } 13, 31 -- for {microscopy-at-microscopy.com} ; Wed, 9 Apr 2014 21:36:41 -0400 } 13, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([169.254.2.193]) by } 13, 31 -- WPVEXCHUB02.purdue.lcl ([172.30.136.64]) with mapi id 14.03.0174.001; Wed, 9 } 13, 31 -- Apr 2014 21:36:41 -0400 } 13, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} } 13, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 13, 31 -- Subject: lignin staining for TEM or SEM } 13, 31 -- Thread-Topic: lignin staining for TEM or SEM } 13, 31 -- Thread-Index: AQHPVF1SFqdF+VAZwkySmcJF426DhQ== } 13, 31 -- Date: Thu, 10 Apr 2014 01:36:40 +0000 } 13, 31 -- Message-ID: {CF6B6E38.1C8B5%dsherman-at-purdue.edu} } 13, 31 -- Accept-Language: en-US } 13, 31 -- Content-Language: en-US } 13, 31 -- X-MS-Has-Attach: } 13, 31 -- X-MS-TNEF-Correlator: } 13, 31 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 } 13, 31 -- x-originating-ip: [24.14.170.252] } 13, 31 -- Content-Type: text/plain; charset="us-ascii" } 13, 31 -- Content-ID: {2124549E5A878A49AF44C34CF8F0D41F-at-exchange.purdue.edu} } 13, 31 -- MIME-Version: 1.0 } 13, 31 -- X-PMX-Version: 6.0.2.2308539 } 13, 31 -- X-PerlMx-URL-Scanned: Yes } 13, 31 -- X-PerlMx-Virus-Scanned: Yes } 13, 31 -- Content-Transfer-Encoding: 8bit } 13, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3A1afBg017682 } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 44 -- From leunissen-at-aurion.nl Thu Apr 10 00:08:16 2014 12, 44 -- Received: from nm2.tnz.bullet.mail.aue.yahoo.com (nm2.tnz.bullet.mail.aue.yahoo.com [124.108.96.29]) 12, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3A58Ere006322 12, 44 -- for {microscopy-at-microscopy.com} ; Thu, 10 Apr 2014 00:08:15 -0500 12, 44 -- Received: from [124.108.96.27] by nm2.tnz.bullet.mail.aue.yahoo.com with NNFMP; 10 Apr 2014 05:08:14 -0000 12, 44 -- Received: from [124.108.96.25] by tm2.tnz.bullet.mail.aue.yahoo.com with NNFMP; 10 Apr 2014 05:08:14 -0000 12, 44 -- Received: from [127.0.0.1] by omp1002.tnz.mail.aue.yahoo.com with NNFMP; 10 Apr 2014 05:08:14 -0000 12, 44 -- X-Yahoo-Newman-Id: 28074.10999.bm-at-omp1002.tnz.mail.aue.yahoo.com 12, 44 -- Received: (qmail 93827 invoked by uid 1000); 10 Apr 2014 05:08:14 -0000 12, 44 -- Received: from 124.108.96.73 by rel105.mail.aue.yahoo.com with SMTP; Thu, 10 Apr 2014 05:08:13 +0000 12, 44 -- Received: (qmail 32223 invoked from network); 10 Apr 2014 05:08:13 -0000 12, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1397106493; bh=id6EMvozH1atDf5/TJtcVuscv8iY+hWDu0/VbwbkSBU=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:Content-Type:Mime-Version:Subject:From:In-Reply-To:Date:Cc:Content-Transfer-Encoding:Message-Id:References:To:X-Mailer; b=S9ehdqMxihuQ1VyNuIq7m6Ul0LRwquHLZIyVvfdhR04vsDa13paeXnHa4D5dK+QocMCZ6wxVNV7+ZM5kFqDR8KSj7DGF9Zk4wNEDATTXGeoYB1w9dIg8S1ZLpQ6+uk4g1MEOB41Ij6hmyH/zhiawP0vuAuRq6vDRbqgc4BiGQEM= 12, 44 -- X-Yahoo-Newman-Property: ymail-3 12, 44 -- X-YMail-OSG: ACsGQXAVM1kYW2g5S1FhezDqMzs8IeZCuU_85U5KBElkTvz 12, 44 -- Lym3zTxoPgDBUizkNjcl64QTnRlblCkFh5uEnh5FI1f1FlZfRnIlD8GJlUGn 12, 44 -- WRVgaCwVy4.xXhf5GcvqhQWidaMbODmXrB.yA8CbRm1p7.YEBwLlr1X52gU4 12, 44 -- qzEARAv66I9RylmsMczIkxyMccqxsOxen_2spxT7hnJTDMSCkMBlhVzv0ACL 12, 44 -- vTOptp.4Lj5LjZNqNS1t6ENoKFYzlNSR8L1iVqpT.KMDeJsXpq9cme64H68N 12, 44 -- EoLzKabxOXzrCT01WAd7MEX0njqa6WpsqiPN8GZ4B2bu6goUk2K7SowAq0fw 12, 44 -- eDFFV.dduOz6HZGWXkRckjtHEt0OR2XFgEZd0ksOSmCS2p_USq78MwWtXDoq 12, 44 -- iRn0wDsDkXKnOvQws4Gtje2.He1IHBEnaZ1s3iW.RnHva9NlNl61tG2Pd33d 12, 44 -- hCA7DLWlvfUCS9ZXBPgiaWsj9XYVpRlZA.jwcEnsWag_haa33EeDIKMx7Qym 12, 44 -- TH316XU3YdSxyjPkcczjBw_DAI1Ak938YfaNG7eYjDvYUGLn.6YZp6j2P_bo 12, 44 -- Fsb40_DmthnATVwMBWLM1Ab8fcvLfv_0fFwI8Nx4sYPrl6ueE5T.NHdn_jTH 12, 44 -- 2YSYtIuyLiNLsANTf83C38q92OTSOLFDeCyY0HPYEj2EfRK7CKVifWWY8Cnr 12, 44 -- 4hEkc3jl_CiVSz5fteUH4vmJco_4zBPdAiNiXiCsq_5u2ywNPP7yTQ4uepc0 12, 44 -- .NwriMAKH0_WpyjJYcmvx5bMKbZoQ_sWCi_lRe2aTw.DcU40EMXboI7omjQQ 12, 44 -- tYq1W 12, 44 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 12, 44 -- X-Rocket-Received: from server.telecom (leunissen-at-125.238.230.13 with plain [124.108.96.158]) 12, 44 -- by smtp104.tnz.mail.aue.yahoo.com with SMTP; 09 Apr 2014 22:08:13 -0700 PDT 12, 44 -- Content-Type: text/plain; charset=us-ascii 12, 44 -- Mime-Version: 1.0 (Mac OS X Mail 7.2 \(1874\)) 12, 44 -- Subject: Re: [Microscopy] lignin staining for TEM or SEM 12, 44 -- From: Jan Leunissen {leunissen-at-aurion.nl} 12, 44 -- In-Reply-To: {201404100137.s3A1b4C6017950-at-ns.microscopy.com} 12, 44 -- Date: Thu, 10 Apr 2014 17:08:09 +1200 12, 44 -- Cc: Debby Sherman {dsherman-at-purdue.edu} 12, 44 -- Message-Id: {A2B0BA24-0192-41F0-8895-BD272E9C3912-at-aurion.nl} 12, 44 -- References: {201404100137.s3A1b4C6017950-at-ns.microscopy.com} 12, 44 -- To: microscopy-at-microscopy.com 12, 44 -- X-Mailer: Apple Mail (2.1874) 12, 44 -- Content-Transfer-Encoding: 8bit 12, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3A58Ere006322 ==============================End of - Headers==============================
This was rejected when in html format, so reposting plain:
Hi Debby,
My colleague Colleen Macmillan, who has much experience staining for lignin, suggests that the KMnO4 in the Maule stain may be electron-dense enough. It certainly highlights cell membranes for TEM. I've pasted her protocol below in case attachments won't go through: _____________________________________________________ Mäule reaction lignin histology of plant sections
Samples: Easiest to place the (fresh) sections in a 48-well/multi-format plate, then add and remove solutions from the sections using narrow-ended plastic transfer pipettes.
Safety: Perform these reactions in a fume-hood, and wear nitrile gloves, safety glasses, labcoat etc.
Mäule reaction protocol: 1. incubate sections (fresh is best) in KMnO-4 (1% aqueous) for 10 min (if very thin sections then a couple of min is fine) 2. wash sections (ddH20) 3. acidify with conc. HCl (37%) for 1 min 4. wash again (ddH20) 5. incubate in NaHCO-3 (5% w/v; fresh) ~2-5 mins, or until colour develops [then mount, view, photograph etc.]
Indications:
red = syringyl lignin brown = guaiacyl lignin
Based on pers. comm. from Armand Seguin + reference = Sibout, R., A. Eudes, et al. (2005). "Cinnamyl alcohol dehydrogense C and D are the primary genes involved in lignin biosynthesis in the floral stem of Arabidopsis." Plant Cell 17(7): 2059-2076.
Mechanism: ŚChlorination of the syringyl nucleus leads to a pink (lignifying cells) or red (lignified cells) color, whereas the guaiacyl nucleus produces a light (lignifying cells) to dark (lignified cells) brown color (Bland, 1966; Wardrop, 1981).ą quote from US Patent Issued on October 30, 2007, Inventor(s) Laigeng Li, Vincent Lee C. Chiang
Good luck, cheers, Rosemary White
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
with regard to Rosemary White’s post I would like to point you to an indirect method to perhaps “localize” lignin in ultrathin sections (using UO2Ac-KMnO4-staining, see below)... There also has been made use of KMnO4 in the "double" staining. Thanks to Rosemary for including the recipe for the Mäule-(Maeule)-Reaction!.
For your convenience I've copied and pasted part of the {Methods-section} , i.e. for TEM of Cell wall ultrastructure: X-from: American Journal of Botany 94(6): 912–925. 2007 REACTION TISSUE FORMATION AND STEM TENSILE MODULUS PROPERTIES IN WILD-TYPE AND P-COUMARATE-3-HYDROXYLASE DOWNREGULATED LINES OF ALFALFA,MEDICAGO SATIVA (FABACEAE)
ANN M. PATTEN, MICHAE¨L JOURDES, ELVIE E. BROWN, MARIE-PIERRE LABORIE, LAURENCE B. DAVIN, AND NORMAN G. LEWIS Cf. also http://www.ncbi.nlm.nih.gov/pubmed/21636460 )
{ { Cell wall ultrastructure— Identification of reaction vs. normal cell types was further confirmed using transmission electron microscopy (TEM). Samples were harvested from a mature internode (IN20) from both WT and pC3H-I lines, as well as from branch tissue of black cottonwood as a control. Tissues (ca. 5 mm2) were fixed in 2% paraformaldehyde and 1.25% glutaraldehyde in 50 mm piperazine-1,4-bis(2-ethanesulphonic acid) (PIPES) buffer (pH 7.2) overnight at 48C. Samples were dehydrated using a standard ethanol series, gradually infiltrated with LR White resin (London Resin, Reading, UK), and heat cured. Thin sections were obtained using a diamond knife mounted to a Reichert Ultracut R ultramicrotome (Reichert-Jung GmbH, Heidelberg, Germany), and sections were mounted on formvar-coated 200-mesh nickel grids.
........Sections were stained with a 3:1 dilution of 4% (w/v) uranyl acetate and 1% (w/v) KMnO4 ........
and observed at 100 kV using a JEOL JEM-1200 EX transmission electron microscope (JEOL, Tokyo, Japan).} }
If you would like to get the .pdf for your personal use only(Copyright issue), please advise/request by reply to me. Disclaimer: I haven't used the method so far, not working on wood or lignin localization....
Best wishes and regards, Wolfgang
Von: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Gesendet: Donnerstag, 10. April 2014 07:26 An: Muß Wolfgang Betreff: [Microscopy] Re: lignin for TEM/SEM? ==} KMnO4-in Mäule-reaction
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This was rejected when in html format, so reposting plain:
Hi Debby,
My colleague Colleen Macmillan, who has much experience staining for lignin, suggests that the KMnO4 in the Maule stain may be electron-dense enough. It certainly highlights cell membranes for TEM. I've pasted her protocol below in case attachments won't go through: _____________________________________________________
Mäule reaction lignin histology of plant sections
Samples: Easiest to place the (fresh) sections in a 48-well/multi-format plate, then add and remove solutions from the sections using narrow-ended plastic transfer pipettes.
Safety: Perform these reactions in a fume-hood, and wear nitrile gloves, safety glasses, labcoat etc.
Mäule reaction protocol: 1. incubate sections (fresh is best) in KMnO4 (1% aqueous) for 10 min (if very thin sections then a couple of min is fine) 2. wash sections (ddH20) 3. acidify with conc. HCl (37%) for 1 min 4. wash again (ddH20) 5. incubate in NaHCO3 (5% w/v; fresh) ~2-5 mins, or until colour develops [then mount, view, photograph etc.]
Indications:
red = syringyl lignin brown = guaiacyl lignin
Based on pers. comm. from Armand Seguin + reference = Sibout, R., A. Eudes, et al. (2005). "Cinnamyl alcohol dehydrogense C and D are the primary genes involved in lignin biosynthesis in the floral stem of Arabidopsis." Plant Cell 17(7): 2059-2076.
Mechanism: ŚChlorination of the syringyl nucleus leads to a pink (lignifying cells) or red (lignified cells) color, whereas the guaiacyl nucleus produces a light (lignifying cells) to dark (lignified cells) brown color (Bland, 1966; Wardrop, 1981).ą quote from US Patent Issued on October 30, 2007, Inventor(s) Laigeng Li, Vincent Lee C. Chiang
Good luck, cheers, Rosemary White
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
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Email: rpowell-at-nanoprobes.com Name: Richard Powell
Organization: Nanoprobes, Incorporated
Title-Subject: [Filtered] Re: [Microscopy] TEM ImmunoGold workshops or courses offered in the USA?
Message: Hello David:
One of the short courses at Microscopy & Microanalysis 2014, "Immunolabeling Technology for Light and Electron Microscopy" will cover immunogold labeling in detail (disclosure: I will be one of the instructors, and I work for Nanoprobes, a company that makes immunogold probes):
Some of the other companies that make immunogold products also hold workshops and courses; I suggest checking the web sites of manufacturers to see if there is an upcoming workshop or course near you.
Hope this helps,
Rick Powell Nanoprobes, Incorporated
Greetings All,
I was hoping to get any information regarding immuno-gold labeling workshops/courses being offered this year in The U.S. I currently have quite a bit of experience in conventional biological TEM work but was hoping to expand my capacity to include immuno gold labeling. I have no experience with this technique and was hoping some sort of course or workshop might exist.
Kind Regards,
David Cugier, Associate Scientist II Investigative Tox/Path-Preclinical Safety Division Dept. R45M AbbVie, Inc. 1 North Waukegan Road North Chicago, Illinois 60064
Phone 847-938-6725 David.Cugier-at-Abbvie.com
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Hi,
We have an opening for an Electron Microscopy service engineer in the Center for Cellular Imaging and NanoAnalytics (C-CINA) of the Biozentrum of the University of Basel (http://c-cina.org). We operate several TEM and SEM electron microscopes, including a FEI Titan Krios with a Gatan K2 Summit direct electron detector, FEI Polara, CM200FEG, T12-Autoloader, CM10, Helios FIB-SEM and a Quanta200FEG with a Gatan 3View.
We have an opening for an
========================= EM Service Engineer =========================
Your responsibilities will include the maintenance and repair of the electron microscopes. We do not have any factory maintenance contracts but may request additional help from the factory when needed. Responsibilities of this position are maintenance and repair of the electron microscopes including; problem diagnosis, ordering replacement parts from the factory, and repair. This includes: the Titan Krios Autoloader, cryo-stages, FEG and high voltage supplies, vacuum systems, mechanics, electronics and software backups.
In addition, upon interest, you would be welcome to participate in method development and research projects, if you were interested. We have an excellent mechanical and electronics workshop available, and are also developing methods in our group. Our research concerns high-resolution structure determination of biological molecules.
Salary and living conditions in Basel are attractive. The lab language is English.
Your profile Experience in EM service for TEM, ideally also for SEM and FIB. Hardware repair experience.
We offer This is a full-time position that is funded directly by the Rektorat of the University of Basel. It is a permanent position in a University setting in one of the most beautiful countries in Europe. We offer a welcoming working environment in an international and multidisciplinary group covering biochemistry, electron microscopy, structural biology, and data analysis. We are an equal opportunity employer.
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For further information, please contact (CONFIDENTIALITY IS ASSURED):
Henning Stahlberg, Director Center for Cellular Imaging and NanoAnalytics (C-CINA) Prof. for Structural Biology and Biophysics, Biozentrum, WRO-1058, Mattenstrasse 26 University of Basel, CH-4058 Basel, Switzerland Tel: +41-61-387 32 62 ; http://c-cina.org mailto:Henning.Stahlberg-at-unibas.ch
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Just in case you missed last week's webinar on standardizing color integrity in your brightfield color images and on your monitors using the new ChromaCal system, the webinar is now available on line:: http://scientific.datacolor.com/watch-recording-apr-2-webinar-ensuring-color-integrity-datacolor-chromacal/?afftid=701G0000000tVqU
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Email: escanley-at-gmail.com Name: Ellen Scanley
Organization: Southern Connecticut State University
I am wondering what types of things people are doing to make sure that their flash drives are virus and malware free?
Our microscopes are not networked for cyber-security purposes but we do use flash drives to download data and sometimes to upload files to the microscope computers.
Thanks for suggestions !
Best Regards, Ellen
Ellen Scanley, MD, PhD Southern Connecticut State University ConnSCU - Center for Nanotechnology 501 Crescent St. New Haven, CT 06515
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Apr 11 17:42:24 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3BMgOgA022882 17, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Apr 2014 17:42:24 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id CA77F6E87D0 17, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Apr 2014 17:42:20 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id eN73ucpJgy2w for {microscopy-at-microscopy.com} ; 17, 28 -- Fri, 11 Apr 2014 17:41:45 -0500 (CDT) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 4D8D86E87BD 17, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Apr 2014 17:41:45 -0500 (CDT) 17, 28 -- Message-ID: {53486FAA.4060309-at-microscopy.com} 17, 28 -- Date: Fri, 11 Apr 2014 17:41:46 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Flash Drive virus/malware protection 17, 28 -- References: {201404110052.s3B0qwW7014328-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201404110052.s3B0qwW7014328-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201404110052.s3B0qwW7014328-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From express_drugstore9-at-tedpro.com Sat Apr 12 04:36:22 2014 Return-Path: {express_drugstore9-at-tedpro.com} Received: from mail3.tedpro.com ([193.242.177.254]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3C9aJE6021310 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 12 Apr 2014 04:36:20 -0500 Message-Id: {201404120936.s3C9aJE6021310-at-ns.microscopy.com}
I would be hesitant in using flash drives. You could set up a system to scan them in another computer before plugging them into the microscope computer. However, even if you officially have a protocol in place to scan the drives before they go into the microscope computer, it wouldn't take but a single infection to ruin your day.
I think a better solution would be to attach the instrument computer to another computer that would serve as a firewall between the microscope and the broader network. Users could save their files to the intermediary and then use their method of choice to retrieve them. They could use a thumb drive or share them over the network. FEI, at least, uses such an approach for their microscopes and we have adopted the approach for other instrumentation running on old computers.
Warren
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, April 11, 2014 5:43 PM To: Straszheim, Warren E [BIOTC]
X-from: escanley-at-gmail.com ()
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both escanley-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: escanley-at-gmail.com Name: Ellen Scanley
Organization: Southern Connecticut State University
I am wondering what types of things people are doing to make sure that their flash drives are virus and malware free?
Our microscopes are not networked for cyber-security purposes but we do use flash drives to download data and sometimes to upload files to the microscope computers.
Thanks for suggestions !
Best Regards, Ellen
Ellen Scanley, MD, PhD Southern Connecticut State University ConnSCU - Center for Nanotechnology 501 Crescent St. New Haven, CT 06515
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Apr 11 17:42:24 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3BMgOgA022882 17, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Apr 2014 17:42:24 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id CA77F6E87D0 17, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Apr 2014 17:42:20 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id eN73ucpJgy2w for {microscopy-at-microscopy.com} ; 17, 28 -- Fri, 11 Apr 2014 17:41:45 -0500 (CDT) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 4D8D86E87BD 17, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Apr 2014 17:41:45 -0500 (CDT) 17, 28 -- Message-ID: {53486FAA.4060309-at-microscopy.com} 17, 28 -- Date: Fri, 11 Apr 2014 17:41:46 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Flash Drive virus/malware protection 17, 28 -- References: {201404110052.s3B0qwW7014328-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201404110052.s3B0qwW7014328-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201404110052.s3B0qwW7014328-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From drugstore_affordable3-at-harrisnesbitt.com Sun Apr 13 04:37:28 2014 Return-Path: {drugstore_affordable3-at-harrisnesbitt.com} Received: from mail2.harrisnesbitt.com (188.231.192.253.freenet.com.ua [188.231.192.253] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3D9bQ33003225 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 13 Apr 2014 04:37:26 -0500 Message-Id: {201404130937.s3D9bQ33003225-at-ns.microscopy.com}
In our facility, we have two dedicated, sterile flash drives to move data around within the facility, but only use a server or Google Drive for data leaving the facility. The best way to make sterile flash drives to to buy new drives, and then perform a long format on a computer with a fresh OS install.
In my experience, if users are bringing in their own drives to transfer data, it is inevitable that your equipment will become infected. To make our systems secure over the internet, the server is fire-walled to only allow access via the university subnet, and the routers are whitelisted to only allow access via ports 22, 80, and 443. We also have the internet browsers locked down to only allow access to Google Drive to keep people from surfing the internet on the computers.
We also have a wireless guest network on the router, such that users can still get the internet on their own devices, but cannot get access to the protected NAT.
We've had this system in place for over a year and have had very good success with it so far.
Hope this helps, Ben Smith
Benjamin E. Smith, Ph.D. Samuel Roberts Noble Microscopy Laboratory University of Oklahoma Norman, OK 73019 E-mail: benjamin.smith-at-ou.edu Voice 405-325-4391 FAX 405-325-7619 http://www.microscopy.ou.edu/ ________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Friday, April 11, 2014 5:57 PM To: Smith, Benjamin E.
X-from: escanley-at-gmail.com ()
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both escanley-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: escanley-at-gmail.com Name: Ellen Scanley
Organization: Southern Connecticut State University
I am wondering what types of things people are doing to make sure that their flash drives are virus and malware free?
Our microscopes are not networked for cyber-security purposes but we do use flash drives to download data and sometimes to upload files to the microscope computers.
Thanks for suggestions !
Best Regards, Ellen
Ellen Scanley, MD, PhD Southern Connecticut State University ConnSCU - Center for Nanotechnology 501 Crescent St. New Haven, CT 06515
Login Host: 108.208.124.229 Listserver Email Form V - 20120416 --------------------------------------------------------------------------- ---===[|]===---
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
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==============================Original Headers============================== 26, 29 -- From benjamin.smith-at-ou.edu Mon Apr 14 08:27:08 2014 26, 29 -- Received: from et1.ou.edu (et1.ou.edu [129.15.0.239]) 26, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3EDR87c014851 26, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Apr 2014 08:27:08 -0500 26, 29 -- Received: from it-helium.sooner.net.ou.edu (10.254.250.17) by 26, 29 -- it-freestyle.exch2010-forward.nor.ouint (10.254.250.7) with Microsoft SMTP 26, 29 -- Server (TLS) id 14.3.181.6; Mon, 14 Apr 2014 08:42:04 -0500 26, 29 -- Received: from IT-AIRSTORM.sooner.net.ou.edu ([fe80::7c13:a719:3a9c:9c52]) by 26, 29 -- it-helium.sooner.net.ou.edu ([::1]) with mapi id 14.03.0181.006; Mon, 14 Apr 26, 29 -- 2014 08:27:07 -0500 26, 29 -- From: "Smith, Benjamin E." {benjamin.smith-at-ou.edu} 26, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 26, 29 -- CC: "escanley-at-gmail.com" {escanley-at-gmail.com} 26, 29 -- Subject: FW: [Microscopy] viaWWW:Flash Drive virus/malware protection 26, 29 -- Thread-Topic: [Microscopy] viaWWW:Flash Drive virus/malware protection 26, 29 -- Thread-Index: AQHPVdlfQV//RxESq0aBY8MBRgRLtZsRGvO6gAADOmo= 26, 29 -- Date: Mon, 14 Apr 2014 13:27:07 +0000 26, 29 -- Message-ID: {9AEFBE3ACCC5714D94A0C452201606076E503013-at-IT-AIRSTORM.sooner.net.ou.edu} 26, 29 -- References: {201404112257.s3BMvAZl007851-at-ns.microscopy.com} , {9AEFBE3ACCC5714D94A0C452201606076E503002-at-IT-AIRSTORM.sooner.net.ou.edu} 26, 29 -- In-Reply-To: {9AEFBE3ACCC5714D94A0C452201606076E503002-at-IT-AIRSTORM.sooner.net.ou.edu} 26, 29 -- Accept-Language: en-US 26, 29 -- Content-Language: en-US 26, 29 -- X-MS-Has-Attach: 26, 29 -- X-MS-TNEF-Correlator: 26, 29 -- x-originating-ip: [129.15.161.16] 26, 29 -- Content-Type: text/plain; charset="us-ascii" 26, 29 -- MIME-Version: 1.0 26, 29 -- Content-Transfer-Encoding: 8bit 26, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3EDR87c014851 ==============================End of - Headers==============================
Hello Ellen, In our facility, we have microscope PC on a local network and one other PC running Linux OS (Debian Wheezy). This PC is connected via SAMBA to the microscope PC (read-only shared folders with guest's images). Every user has a possibility to copy images from microscope PC onto her/his flash drive on Linux box. No uploads to microscope PC are permitted for normal user (guest).
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
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Does anyone have some sage advise about how microscopy and biotechnology can be integrated?
I have a microscopy training program, another nearby campus has a biotech program. We would like to find some common ground so our students can benefit from learning both.
This may seem like a silly question, after all, where would any science be without microscopes :).
But we are putting together a poster to show new students how these areas can overlap, so if you have any good ideas, send them my way.
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 18, 37 -- From prvs=1174990319=jkrupp-at-deltacollege.edu Mon Apr 14 16:03:31 2014 18, 37 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.237]) 18, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id s3EL3VSq026090 18, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Apr 2014 16:03:31 -0500 18, 37 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 18, 37 -- by localhost (Email Security Appliance) with SMTP id 4E3F19899B5_34C4D20B; 18, 37 -- Mon, 14 Apr 2014 21:03:28 +0000 (GMT) 18, 37 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) 18, 37 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 46ED7989972_34C4D20F; 18, 37 -- Mon, 14 Apr 2014 21:03:28 +0000 (GMT) 18, 37 -- Received: from localhost (localhost [127.0.0.1]) 18, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id BE3C2D104DD; 18, 37 -- Mon, 14 Apr 2014 14:03:26 -0700 (PDT) 18, 37 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 18, 37 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 18, 37 -- with ESMTP id tGSGuLccy7Bb; Mon, 14 Apr 2014 14:03:21 -0700 (PDT) 18, 37 -- Received: from localhost (localhost [127.0.0.1]) 18, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 40E7ED104D2; 18, 37 -- Mon, 14 Apr 2014 14:03:14 -0700 (PDT) 18, 37 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 18, 37 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 18, 37 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 18, 37 -- with ESMTP id 9UfY1IIjXtwe; Mon, 14 Apr 2014 14:03:14 -0700 (PDT) 18, 37 -- Received: from cmas117-01.academic.server.sjdc (unknown [10.146.100.11]) 18, 37 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id A7229D104E2; 18, 37 -- Mon, 14 Apr 2014 14:02:38 -0700 (PDT) 18, 37 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 18, 37 -- Content-Type: text/plain; charset=us-ascii 18, 37 -- Subject: Microscopy & Biotechnology question 18, 37 -- Date: Mon, 14 Apr 2014 14:02:38 -0700 18, 37 -- Message-Id: {DDCC6BBC-C42F-4B3E-B328-2F5A4A441509-at-deltacollege.edu} 18, 37 -- Cc: Kristen {kristenjy8-at-gmail.com} , Heather Brown {BrownH-at-arc.losrios.edu} 18, 37 -- To: Microscopy-at-microscopy.com 18, 37 -- Mime-Version: 1.0 (Apple Message framework v1085) 18, 37 -- X-Mailer: Apple Mail (2.1085) 18, 37 -- Content-Transfer-Encoding: 8bit 18, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3EL3VSq026090 ==============================End of - Headers==============================
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Email: margaret.bisher-at-gmail.com Name: Margaret Bisher
Organization: University of Pittsburgh School of Medicine
Title-Subject: [Filtered] Offer: Microscopy Books
Message: Trying to thin out my library and I was wondering if anyone would want some/all of these books?
Principle and Practice of Electron Microscope Operation Low Temperature Methods in Biological Electron Microscopy Electron Diffraction: An Introduction for Biologists Design of the Electron Microscopy Laboratory Image Analysis, Enhancement and Interpretation - all of these are from the series: Practical Methods in Electron Microscopy
And I also have Vacuum Methods in Electron Microscopy, by Wilbur C. Bigelow.
The books are free, all I ask is that you pay the shipping costs.
Thank you, Margaret Bisher
University of Pittsburgh School of Medicine Department of Cell Biology S346 Biomedical Science Tower Pittsburgh, PA 15213
email: meb187-at-pitt.edu phone: 412-648-9565
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We have installed USB Drive AntiVirus on our computers that scans flash drives and supposedly prevents them from infecting the computers.
David
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Message: Jon, Perhaps the following may serve as an inspiration to you and your colleagues: http://blog.neuinfo.org/index.php/news-events/general-information/vasculature-morphogenesis-synopsis-of-three-related-articles-by-halina-witkiewicz-phil-oh-and-jan-schnitzer.
Regards, Halina
} From: jkrupp-at-deltacollege.edu [jkrupp-at-deltacollege.edu] Sent: Monday, April 14, 2014 2:11 PM To: Witkiewicz, Halina
OK, help me with this.
Does anyone have some sage advise about how microscopy and biotechnology can be integrated?
I have a microscopy training program, another nearby campus has a biotech program. We would like to find some common ground so our students can benefit from learning both.
This may seem like a silly question, after all, where would any science be without microscopes :).
But we are putting together a poster to show new students how these areas can overlap, so if you have any good ideas, send them my way.
Thanks
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
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==============================Original Headers============================== 25, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 14 18:25:01 2014 25, 28 -- Received: from znl.com ([206.69.208.20]) 25, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3ENP1Kk018009 25, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Apr 2014 18:25:01 -0500 25, 28 -- Received: from localhost (localhost [127.0.0.1]) 25, 28 -- by znl.com (Postfix) with ESMTP id A1AFA6EABD9 25, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Apr 2014 18:25:01 -0500 (CDT) 25, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 25, 28 -- Received: from znl.com ([127.0.0.1]) 25, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 25, 28 -- with ESMTP id vdl8F6uyhyfp for {microscopy-at-microscopy.com} ; 25, 28 -- Mon, 14 Apr 2014 18:24:55 -0500 (CDT) 25, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 25, 28 -- by znl.com (Postfix) with ESMTPA id B11C76EABCC 25, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Apr 2014 18:24:55 -0500 (CDT) 25, 28 -- Message-ID: {534C6E4E.6040203-at-microscopy.com} 25, 28 -- Date: Mon, 14 Apr 2014 18:25:02 -0500 25, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 25, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 25, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 25, 28 -- MIME-Version: 1.0 25, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 25, 28 -- Subject: viaWWW:Microscopy & Biotechnology question 25, 28 -- References: {201404142159.s3ELx1ls012994-at-ns.microscopy.com} 25, 28 -- In-Reply-To: {201404142159.s3ELx1ls012994-at-ns.microscopy.com} 25, 28 -- X-Forwarded-Message-Id: {201404142159.s3ELx1ls012994-at-ns.microscopy.com} 25, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 25, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From drugs-express7-at-mail2moon.com Mon Apr 14 23:05:12 2014 Return-Path: {drugs-express7-at-mail2moon.com} Received: from mail1.mail2moon.com ([117.204.24.23]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3F456Dp008639 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 14 Apr 2014 23:05:07 -0500 Message-Id: {201404150405.s3F456Dp008639-at-ns.microscopy.com}
Jon,
Drosophila embryos. One of our profs works on shrimp development - fertilization through juveniles, and they use both molecular biology methods chasing the relevant genes, Western blots and all that, and confocal microscopy to see exactly where the genes are being expressed. Which cells and where within the cells. Something molecular techniques don't tell you. And, since he teaches the confocal class, he's used the same methods to demonstrate the various homeobox genes in Drosophila embryos. Do the molecular biology to get the information it provides, then image the embroyos. Really nifty segmental patterns. (Make "what does molecular biology/microscopy tell me?" and "how does the one relate to the other?" part of the assignment. Don't tell them, let them discover the relationships.)
Another does similar things with C. elegans. Two model organisms well suited for class work, with lots of information Out There.
You could do similar things with cells in culture, following gene expression through protein synthesis and transport between cellular compartments. Run the techniques in parallel.
You're right, though - the techniques integrate well.
Phil
} OK, help me with this. } } Does anyone have some sage advise about how microscopy and biotechnology can be integrated? } } I have a microscopy training program, another nearby campus has a biotech program. We would like to find some common ground so our students can benefit from learning both. } } This may seem like a silly question, after all, where would any science be without microscopes :). } } But we are putting together a poster to show new students how these areas can overlap, so if you have any good ideas, send them my way. } } Thanks } } Jon } } Jonathan Krupp } Applied Science, Business& Technology } San Joaquin Delta College } 5151 Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } Find us on Facebook -at- } Electron Microscopy at SJ Delta College -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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Does anyone have a favorite freeze-substitution recipe that enhances the staining of the plasma membrane and tonoplast? I usually use Osmium and UA in acetone. Is there something else I can add to the nasty cocktail to enhance the membranes? thanks for any help, Beth
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Email: zhouhw33-at-gmail.com Name: Hongwen Zhou
Organization: Applied Materials
Title-Subject: [Filtered] Vacuum leak in JEOL ARM200F TEM
Message: We have a JEOL ARM200F TEM in our lab. The system has two vacuum-related issues, which lead to only ~ 30% up time since we purchased it last year. The first one is that the chamber vacuum had crashed at least five times when we were filling the liquid-N2 dewar. The second one is that the samples are contaminated (by carbon) very quickly when we take images and/or do EELS/EDS analysis under STEM mode. All samples are plasma-cleaned before inserting them into the ARM. And, we have not observed such high contamination rate for the same samples in other TEMs. We detected two leaking spots (by Helium leak test) on the goniometer and suspect those might be the root-cause of carbon contamination. JEOL recommends us beam-shower the samples before STEM imaging/analysis, but that destroys some of the samples and changes the structure of the materials in some cases.
I wonder if anyone had done any study on the correlation between carbon contamination (under STEM) and low level vacuum leak before. Is it a pre-requisite to beam-shower the samples to obtain atomic resolution images and high-quality EELS/EDS results? Thank you in advance for your input.
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Title-Subject: [Filtered] E-mail to the list with job advertisement
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Hi
I think you will have realised that you have an "O" ring problem on two items that sit in the specimen area. It is extremely important to solve these two problems before trying to characterise the vacuum system and the contamination.
Another point, a leak in the specimen area may also cause etching of specimens, reducing their cross section due to gas ionisation. For example a holey carbon film may be reduced to the structure of a string vest in minutes due to etching.
So, fix the problems that you have found, and the contamination problem may well go away.
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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Email: zhouhw33-at-gmail.com Name: Hongwen Zhou
Organization: Applied Materials
Title-Subject: [Filtered] Vacuum leak in JEOL ARM200F TEM
Message: We have a JEOL ARM200F TEM in our lab. The system has two vacuum-related issues, which lead to only ~ 30% up time since we purchased it last year. The first one is that the chamber vacuum had crashed at least five times when we were filling the liquid-N2 dewar. The second one is that the samples are contaminated (by carbon) very quickly when we take images and/or do EELS/EDS analysis under STEM mode. All samples are plasma-cleaned before inserting them into the ARM. And, we have not observed such high contamination rate for the same samples in other TEMs. We detected two leaking spots (by Helium leak test) on the goniometer and suspect those might be the root-cause of carbon contamination. JEOL recommends us beam-shower the samples before STEM imaging/analysis, but that destroys some of the samples and changes the structure of the materials in some cases.
I wonder if anyone had done any study on the correlation between carbon contamination (under STEM) and low level vacuum leak before. Is it a pre-requisite to beam-shower the samples to obtain atomic resolution images and high-quality EELS/EDS results? Thank you in advance for your input.
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Dear All, maybe there is someone out there before going into Easter holidays to share a bit of knowledge. I just founds a strange detail on a specimen (the upper leaf surface of a cucumber plant): See images here: www.electronmicroscopy.info/customers/cucumber/index.htm There is also a PDF with EDS data, but it is not very specific since I put a whole lot of Platinum on the surface because of a nanoflight movie I did with this surface: www.electronmicroscopy.info/customers/cucumber/cucumber_eds.pdf A little bit of Sulfur and Ca is showing up, with Carbon. Any idea what this might be?
Best wishes, happy Easter, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Hongwen; We have had the column vacuum crashed on only one occasion, and that was when an inexperienced user filled the ACD to overfilling, and did not realize it was overflowing, so he poured and poured, which we believe froze the "O" ring that seals the top of the ACD. Aside from that, the column has never crashed. Contamination was a problem when we were heating the ACD every night. When we keep the ACD cold 24 hours a day, which can be easily done on the ARM, contamination from the column is eliminated. Contamination in a high current STEM (the ARM delivers ~10X the beam current density of a 2010F) is always a bigger problem than it is in conventional microscopes. We still see specimen-born contamination, and plasma cleaning does not seem to help as much as we would like. The best way to clean dirty specimens is baking them. We use the baking system that came with our NION UltraSTEM for samples that cannot be successfully cleaned by plasma cleaning.
John Mardinly, ASU
-----Original Message-----
Email: zhouhw33-at-gmail.com Name: Hongwen Zhou
Organization: Applied Materials
Title-Subject: [Filtered] Vacuum leak in JEOL ARM200F TEM
Message: We have a JEOL ARM200F TEM in our lab. The system has two vacuum-related issues, which lead to only ~ 30% up time since we purchased it last year. The first one is that the chamber vacuum had crashed at least five times when we were filling the liquid-N2 dewar. The second one is that the samples are contaminated (by carbon) very quickly when we take images and/or do EELS/EDS analysis under STEM mode. All samples are plasma-cleaned before inserting them into the ARM. And, we have not observed such high contamination rate for the same samples in other TEMs. We detected two leaking spots (by Helium leak test) on the goniometer and suspect those might be the root-cause of carbon contamination. JEOL recommends us beam-shower the samples before STEM imaging/analysis, but that destroys some of the samples and changes the structure of the materials in some cases.
I wonder if anyone had done any study on the correlation between carbon contamination (under STEM) and low level vacuum leak before. Is it a pre-requisite to beam-shower the samples to obtain atomic resolution images and high-quality EELS/EDS results? Thank you in advance for your input.
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realname - Naveen Rathi Email - mpsnaveen0208-at-gmail.com ORGANIZATION - Ecole Centrale de Nantes EDUCATION - Graduate College LOCATION - Nantes,France SUBJECT_OF_QUESTION - 3D microscopy QUESTION - Is there any robust and fastest way to segment the image in 3D using the Image j software by removing all the artifacts ?
==============================Original Headers============================== 2, 27 -- From oshel1pe-at-cmich.edu Fri Apr 18 07:54:05 2014 2, 27 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3ICs5v6005893 2, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Apr 2014 07:54:05 -0500 2, 27 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75] (may be forged)) 2, 27 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s3ICs4vS024318 2, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Apr 2014 08:54:04 -0400 2, 27 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 2, 27 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 2, 27 -- 14.3.174.1; Fri, 18 Apr 2014 08:54:04 -0400 2, 27 -- Message-ID: {5351206C.2070405-at-cmich.edu} 2, 27 -- Date: Fri, 18 Apr 2014 08:54:04 -0400 2, 27 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 2, 27 -- Reply-To: {mpsnaveen0208-at-gmail.com} 2, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 2, 27 -- MIME-Version: 1.0 2, 27 -- To: micro {Microscopy-at-microscopy.com} 2, 27 -- Subject: Ask-A-Microscopist: 3D segmentation using Image J? 2, 27 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 2, 27 -- Content-Transfer-Encoding: 7bit 2, 27 -- X-Originating-IP: [141.209.160.122] 2, 27 -- X-Bayes-Prob: 0.9656 (Score 4, tokens from: -at--at-RPTN, default) 2, 27 -- X-Spam-Score: 4.20 (****) [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.9656:4.0) 2, 27 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 2, 27 -- X-CanItPRO-Stream: default 2, 27 -- X-Canit-Stats-ID: 05LPoS4KS - 8c6ebb221a91 - 20140418 2, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
From express-drugstore4-at-optisprint.bg Fri Apr 18 08:35:38 2014 Return-Path: {express-drugstore4-at-optisprint.bg} Received: from 212-233-238-127-pleven.optisprint.bg (212-233-238-127-pleven.optisprint.bg [212.233.238.127] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3IDZZ5m025645 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 18 Apr 2014 08:35:36 -0500 Message-Id: {201404181335.s3IDZZ5m025645-at-ns.microscopy.com}
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Email: thomas.burrage-at-dhs.gov Name: Thomas G. Burrage
Organization: DHS S&T Plum Island Animal Disease Center
Title-Subject: [Filtered] EM Fellowship announcement
Message: Electron Microscopy Fellowship Appointment
Description: The U.S. Department of Homeland Security (DHS) Science and Technology (S&T) Directorate is seeking a motivated scientist (post-master’s or post-baccalaureate) with prior experience and skill sets associated with electron microscopy techniques, or with a strong desire to pursue a scientific career in electron microscopy projects associated with transboundary animal disease viruses (FMDV, ASFV, CSFV). This fellowship is with the Transboundary Animal Disease Countermeasures Branch at the Plum Island Animal Disease Center (PIADC) and is offered through the PIADC Research Participation Program. The position is available immediately and is funded for 1 year, with the opportunity for renewal. PIADC is the only U.S. laboratory facility performing research, development and diagnosis of foreign animal diseases of highest threat to the U.S. This critical national asset is located off the northeast coast of Long Island, NY, and accessible by government-provided ferry from Orient Point, NY, and Old Saybrook, CT. One of the missions of PIADC is to develop technologies to help mitigate the risks of catastrophic economic losses caused by foreign animal disease (FAD) agents accidentally or deliberately introduced into the United States. At PIADC, in a bio-safety level-3 (BSL-3) laboratory environment, the scientist will perform laboratory procedures to visualize and analyze biopsy, tissue culture derived materials using various microscopic procedures ranging from simple light microscopy to transmission electron microscopy (TEM). Training will be provided. The scientist will prepare specimens and samples, operate standard and specialized laboratory equipment, maintain tissue cultures, stay up-to-date regarding technical developments, conduct literature searches on identified topics relevant to the research, and follow and ensure adherence to strict safety procedures and safety checks. Common methodologies employed include but are not limited to: tissue and cell preparation with fixatives and embedding medias, ultrathin sectioning, paraffin-based cryomicrotomy and immunohistochemistry; virus isolation, propagation and titration; preparation of vaccines; ELISAs; evaluation of vaccine potency and efficacy in swine, cattle and mice; and use of several techniques of recombinant DNA technology, real time PCR, protein expression in bacteria and in tissue culture, light and fluorescence microscopy. Qualifications: The candidate must have demonstrated excellent oral and written communication skills and record keeping skills, as the fellow will be working with agricultural select agents. The applicant must be a U.S. Citizen and must be able to obtain a security clearance. How to Apply: Information about the application process and application forms are available on the program Web site: http://www.orau.gov/piadc For Additional Information: For additional information about the PIADC Research Participation Program, please see http://www.orau.gov/piadc.
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==============================Original Headers============================== 13, 26 -- From microscopylistserver-noreply-at-microscopy.com Sun Apr 20 09:49:38 2014 13, 26 -- Received: from znl.com ([206.69.208.20]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3KEnccc011577 13, 26 -- for {microscopy-at-microscopy.com} ; Sun, 20 Apr 2014 09:49:38 -0500 13, 26 -- Received: from localhost (localhost [127.0.0.1]) 13, 26 -- by znl.com (Postfix) with ESMTP id 8BA1F6EEE2E 13, 26 -- for {microscopy-at-microscopy.com} ; Sun, 20 Apr 2014 09:49:38 -0500 (CDT) 13, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 26 -- Received: from znl.com ([127.0.0.1]) 13, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 26 -- with ESMTP id Kwm5TIM5JE7v for {microscopy-at-microscopy.com} ; 13, 26 -- Sun, 20 Apr 2014 09:49:22 -0500 (CDT) 13, 26 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 26 -- by znl.com (Postfix) with ESMTPA id E4F806EEE1E 13, 26 -- for {microscopy-at-microscopy.com} ; Sun, 20 Apr 2014 09:49:21 -0500 (CDT) 13, 26 -- Message-ID: {5353DE71.5040204-at-microscopy.com} 13, 26 -- Date: Sun, 20 Apr 2014 09:49:21 -0500 13, 26 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 26 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 26 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 13, 26 -- MIME-Version: 1.0 13, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 26 -- Subject: viaWWW: Electron Microscopy Fellowship Appointment at Plum Island 13, 26 -- Animal Disease Center (PIADC) 13, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 26 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From popular-pharmacy7-at-childfocus.org Mon Apr 21 04:05:12 2014 Return-Path: {popular-pharmacy7-at-childfocus.org} Received: from smtp1.childfocus.org ([87.229.245.182]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3L9581L032071 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 21 Apr 2014 04:05:09 -0500 Message-Id: {201404210905.s3L9581L032071-at-ns.microscopy.com}
X-from: fetterr-at-janelia.hhmi.org ()
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Email: fetterr-at-janelia.hhmi.org Name: Richard Fetter
Organization: Howard Hughes Medical Institute
Title-Subject: [Filtered] Cressington 328C
Message: Has anyone used the Cressington 328EB Carbon unit to C coat support films on slot grids? And more importantly, does anyone have this EB C evaporator that would be willing to let me do a demo C evaporation on my grids (in the US)?
Thanks for relevant answers.
Richard Fetter Janelia Farm Research Campus HHMI
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==============================Original Headers============================== 16, 27 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 21 07:56:00 2014 16, 27 -- Received: from znl.com ([206.69.208.20]) 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3LCtxsY003321 16, 27 -- for {microscopy-at-microscopy.com} ; Mon, 21 Apr 2014 07:55:59 -0500 16, 27 -- Received: from localhost (localhost [127.0.0.1]) 16, 27 -- by znl.com (Postfix) with ESMTP id C8F6D6EF825 16, 27 -- for {microscopy-at-microscopy.com} ; Mon, 21 Apr 2014 07:55:59 -0500 (CDT) 16, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 27 -- Received: from znl.com ([127.0.0.1]) 16, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 27 -- with ESMTP id t4nc8FpEfbpd for {microscopy-at-microscopy.com} ; 16, 27 -- Mon, 21 Apr 2014 07:55:53 -0500 (CDT) 16, 27 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 27 -- by znl.com (Postfix) with ESMTPA id CF4A06EF813 16, 27 -- for {microscopy-at-microscopy.com} ; Mon, 21 Apr 2014 07:55:53 -0500 (CDT) 16, 27 -- Message-ID: {5355155C.8010405-at-microscopy.com} 16, 27 -- Date: Mon, 21 Apr 2014 07:55:56 -0500 16, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 27 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 27 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 16, 27 -- MIME-Version: 1.0 16, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 27 -- Subject: viaWWW:Cressington 328C 16, 27 -- References: {201404202002.s3KK2Q9O012522-at-ns.microscopy.com} 16, 27 -- In-Reply-To: {201404202002.s3KK2Q9O012522-at-ns.microscopy.com} 16, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From pharmacy-discounted2-at-kmsf.org Mon Apr 21 10:14:09 2014 Return-Path: {pharmacy-discounted2-at-kmsf.org} Received: from smtp1.kmsf.org ([46.98.226.113]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3LFE6O9023943 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 21 Apr 2014 10:14:07 -0500 Message-Id: {201404211514.s3LFE6O9023943-at-ns.microscopy.com}
Hello Fellow Microscopist,
A researcher in Florida is looking for a facility to perform freeze fracture on E. coli membrane vesicles. The are 1) looking at the size but 2) more importantly, looking at the orientation if they membrane is right side out or flipped inside out. They reference a 1974 paper in Journal Bacteriology, Vol 117, No. 2.
My Core lab is more equipped to examine and model the membranes by cryo-TEM and may try that but wondering if anyone is doing freeze fracture anymore. And if so, where?
Best, Karen Kelley University of Florida Interdisciplinary Center for Biotechnology Research
==============================Original Headers============================== 6, 37 -- From vau-at-ufl.edu Mon Apr 21 14:54:10 2014 6, 37 -- Received: from smtp.ufl.edu (smtp-prod02.osg.ufl.edu [128.227.74.218]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3LJs9E1027193 6, 37 -- for {Microscopy-at-Microscopy.Com} ; Mon, 21 Apr 2014 14:54:10 -0500 6, 37 -- X-UFL-GatorLink-Authenticated: authenticated as () with from 10.253.27.146 6, 37 -- Received: from UFEXCH-CASHT05.ad.ufl.edu ([10.253.27.146]) 6, 37 -- by smtp.ufl.edu (8.13.8/8.13.8/3.0.0) with ESMTP id s3LJs7Hd030708 6, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 6, 37 -- for {Microscopy-at-Microscopy.Com} ; Mon, 21 Apr 2014 15:54:09 -0400 6, 37 -- Received: from UFEXCH-MBXN02.ad.ufl.edu ([fe80::4801:abc9:e123:b1bb]) by 6, 37 -- UFEXCH-CASHT05.ad.ufl.edu ([fe80::f867:af46:29b4:e7a%11]) with mapi id 6, 37 -- 14.03.0174.001; Mon, 21 Apr 2014 15:54:08 -0400 6, 37 -- From: "Kelley,Karen L" {vau-at-ufl.edu} 6, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 6, 37 -- Subject: Is anyone have freeze fracture in FL? 6, 37 -- Thread-Topic: Is anyone have freeze fracture in FL? 6, 37 -- Thread-Index: AQHPXZt0+CzjA9sE2kabJuLUU4q9fA== 6, 37 -- Date: Mon, 21 Apr 2014 19:54:07 +0000 6, 37 -- Message-ID: {CF7AEFC2.3F41D%vau-at-ufl.edu} 6, 37 -- Accept-Language: en-US 6, 37 -- Content-Language: en-US 6, 37 -- X-MS-Has-Attach: 6, 37 -- X-MS-TNEF-Correlator: 6, 37 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 6, 37 -- x-originating-ip: [10.36.9.35] 6, 37 -- Content-Type: text/plain; charset="us-ascii" 6, 37 -- Content-ID: {86BDB72685931F409AC131816598C3A0-at-ad.ufl.edu} 6, 37 -- MIME-Version: 1.0 6, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.11.96,1.0.14,0.0.0000 6, 37 -- definitions=2014-04-21_03:2014-04-21,2014-04-21,1970-01-01 signatures=0 6, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 6, 37 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 6, 37 -- engine=7.0.1-1402240000 definitions=main-1404210312 6, 37 -- X-Spam-Level: * 6, 37 -- X-UFL-Spam-Level: * 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3LJs9E1027193 ==============================End of - Headers==============================
Dr. John Rash from Colorado State University, is the best person I know who does freeze fracture. You can find more information from his website -
http://www.cvmbs.colostate.edu/rashlab/
Zhaojie Zhang Director, Jenkins Microscopy Facility University of Wyoming
-----Original Message----- X-from: vau-at-ufl.edu [mailto:vau-at-ufl.edu] Sent: Monday, April 21, 2014 1:59 PM To: Z.J. Zhang
Hello Fellow Microscopist,
A researcher in Florida is looking for a facility to perform freeze fracture on E. coli membrane vesicles. The are 1) looking at the size but 2) more importantly, looking at the orientation if they membrane is right side out or flipped inside out. They reference a 1974 paper in Journal Bacteriology, Vol 117, No. 2.
My Core lab is more equipped to examine and model the membranes by cryo-TEM and may try that but wondering if anyone is doing freeze fracture anymore. And if so, where?
Best, Karen Kelley University of Florida Interdisciplinary Center for Biotechnology Research
==============================Original Headers============================== 6, 37 -- From vau-at-ufl.edu Mon Apr 21 14:54:10 2014 6, 37 -- Received: from smtp.ufl.edu (smtp-prod02.osg.ufl.edu [128.227.74.218]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3LJs9E1027193 6, 37 -- for {Microscopy-at-Microscopy.Com} ; Mon, 21 Apr 2014 14:54:10 -0500 6, 37 -- X-UFL-GatorLink-Authenticated: authenticated as () with from 10.253.27.146 6, 37 -- Received: from UFEXCH-CASHT05.ad.ufl.edu ([10.253.27.146]) 6, 37 -- by smtp.ufl.edu (8.13.8/8.13.8/3.0.0) with ESMTP id s3LJs7Hd030708 6, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 6, 37 -- for {Microscopy-at-Microscopy.Com} ; Mon, 21 Apr 2014 15:54:09 -0400 6, 37 -- Received: from UFEXCH-MBXN02.ad.ufl.edu ([fe80::4801:abc9:e123:b1bb]) by 6, 37 -- UFEXCH-CASHT05.ad.ufl.edu ([fe80::f867:af46:29b4:e7a%11]) with mapi id 6, 37 -- 14.03.0174.001; Mon, 21 Apr 2014 15:54:08 -0400 6, 37 -- From: "Kelley,Karen L" {vau-at-ufl.edu} 6, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 6, 37 -- Subject: Is anyone have freeze fracture in FL? 6, 37 -- Thread-Topic: Is anyone have freeze fracture in FL? 6, 37 -- Thread-Index: AQHPXZt0+CzjA9sE2kabJuLUU4q9fA== 6, 37 -- Date: Mon, 21 Apr 2014 19:54:07 +0000 6, 37 -- Message-ID: {CF7AEFC2.3F41D%vau-at-ufl.edu} 6, 37 -- Accept-Language: en-US 6, 37 -- Content-Language: en-US 6, 37 -- X-MS-Has-Attach: 6, 37 -- X-MS-TNEF-Correlator: 6, 37 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 6, 37 -- x-originating-ip: [10.36.9.35] 6, 37 -- Content-Type: text/plain; charset="us-ascii" 6, 37 -- Content-ID: {86BDB72685931F409AC131816598C3A0-at-ad.ufl.edu} 6, 37 -- MIME-Version: 1.0 6, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.11.96,1.0.14,0.0.0000 6, 37 -- definitions=2014-04-21_03:2014-04-21,2014-04-21,1970-01-01 signatures=0 6, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 6, 37 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 6, 37 -- engine=7.0.1-1402240000 definitions=main-1404210312 6, 37 -- X-Spam-Level: * 6, 37 -- X-UFL-Spam-Level: * 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3LJs9E1027193 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 34 -- From ZZhang-at-uwyo.edu Mon Apr 21 15:25:59 2014 15, 34 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1lp0145.outbound.protection.outlook.com [207.46.163.145]) 15, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3LKPxCW014093 15, 34 -- for {microscopy-at-microscopy.com} ; Mon, 21 Apr 2014 15:25:59 -0500 15, 34 -- Received: from DM2PR05MB463.namprd05.prod.outlook.com (10.141.105.12) by 15, 34 -- DM2PR05MB464.namprd05.prod.outlook.com (10.141.105.24) with Microsoft SMTP 15, 34 -- Server (TLS) id 15.0.921.12; Mon, 21 Apr 2014 20:25:57 +0000 15, 34 -- Received: from DM2PR05MB463.namprd05.prod.outlook.com ([169.254.15.229]) by 15, 34 -- DM2PR05MB463.namprd05.prod.outlook.com ([169.254.15.229]) with mapi id 15, 34 -- 15.00.0921.000; Mon, 21 Apr 2014 20:25:57 +0000 15, 34 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 15, 34 -- To: "vau-at-ufl.edu" {vau-at-ufl.edu} , 15, 34 -- "microscopy-at-microscopy.com" 15, 34 -- {microscopy-at-microscopy.com} 15, 34 -- Subject: RE: [Microscopy] Is anyone have freeze fracture in FL? 15, 34 -- Thread-Topic: [Microscopy] Is anyone have freeze fracture in FL? 15, 34 -- Thread-Index: AQHPXZw1vZbXIx65gkWWkRmSohXUnJscg3ig 15, 34 -- Date: Mon, 21 Apr 2014 20:25:57 +0000 15, 34 -- Message-ID: {f49e4601973242a482775a0e1d916da6-at-DM2PR05MB463.namprd05.prod.outlook.com} 15, 34 -- References: {201404211958.s3LJwdSH032166-at-ns.microscopy.com} 15, 34 -- In-Reply-To: {201404211958.s3LJwdSH032166-at-ns.microscopy.com} 15, 34 -- Accept-Language: en-US 15, 34 -- Content-Language: en-US 15, 34 -- X-MS-Has-Attach: 15, 34 -- X-MS-TNEF-Correlator: 15, 34 -- x-originating-ip: [129.72.147.57] 15, 34 -- x-forefront-prvs: 0188D66E61 15, 34 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(428001)(377424004)(13464003)(377454003)(189002)(199002)(86362001)(46102001)(54356999)(81342001)(76576001)(15975445006)(80976001)(81542001)(99396002)(74316001)(75432001)(83322001)(19580405001)(512954002)(85852003)(50986999)(77982001)(76176999)(4396001)(31966008)(15202345003)(74662001)(74502001)(33646001)(2171001)(83072002)(2656002)(87936001)(19580395003)(79102001)(66066001)(99286001)(92566001)(76482001)(80022001)(20776003)(24736002)(80792004);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR05MB464;H:DM2PR05MB463.namprd05.prod.outlook.com;FPR:E7FD51BF.9DC45309.BE72DF47.80AAC55C.20528;MLV:sfv;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 15, 34 -- received-spf: None (: uwyo.edu does not designate permitted sender hosts) 15, 34 -- Content-Type: text/plain; charset="us-ascii" 15, 34 -- MIME-Version: 1.0 15, 34 -- X-OriginatorOrg: uwyo.edu 15, 34 -- Content-Transfer-Encoding: 8bit 15, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3LKPxCW014093 ==============================End of - Headers==============================
From pharmacy-discounted11-at-kam-telecom.ru Tue Apr 22 00:33:05 2014 Return-Path: {pharmacy-discounted11-at-kam-telecom.ru} Received: from 42.196.kam-telecom.ru (42.196.kam-telecom.ru [86.109.196.42] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3M5X2M0007031 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 22 Apr 2014 00:33:03 -0500 Message-Id: {201404220533.s3M5X2M0007031-at-ns.microscopy.com}
I have a client who wants to have a viscous liquid cryo-microtomed for TEM.
We don't have the equipment to provide such service and the nature of the liquid is still unknown to us. Does anyone who know who can provides this service so I can refer the inquiry to them?
Thanks........
Frank Karl ARDL
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==============================Original Headers============================== 7, 28 -- From frank_karl-at-ardl.com Tue Apr 22 08:09:02 2014 7, 28 -- Received: from cal1-mh675b.smtproutes.com (cal1-mh675b.smtproutes.com [208.70.89.128]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MD92Gk016859 7, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Apr 2014 08:09:02 -0500 7, 28 -- X-Katharion-ID: 1398172123.21970.cal1-mh675 7, 28 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by 7, 28 -- cal1-mh675.smtproutes.com [(192.69.16.47)] with ESMTP via TCP 7, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 22 Apr 2014 13:08:43 +0000 7, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 7, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Tue, 22 7, 28 -- Apr 2014 09:08:41 -0400 7, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 7, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 7, 28 -- {microscopy-at-microscopy.com} 7, 28 -- Date: Tue, 22 Apr 2014 09:08:40 -0400 7, 28 -- Subject: TEM on liquid thin sections 7, 28 -- Thread-Topic: TEM on liquid thin sections 7, 28 -- Thread-Index: Ac9eK/nwYBPTaB+ORh2pNr0Ac1xwEQ== 7, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BF33A0689-at-exchange2k7.ad.ardl.com} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- acceptlanguage: en-US 7, 28 -- Content-Type: text/plain; charset="us-ascii" 7, 28 -- MIME-Version: 1.0 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3MD92Gk016859 ==============================End of - Headers==============================
Not gunpowder, but all kinds of explosives powders - RDX, HMX, HNS, PETN and so forth. We make shaped charges for perforating oil and gas wells.
I usually gold coat to make the powders conductive and improve secondary electron yield, and use an aluminum stub for best thermal conductivity. I have a Lexan shield between me and the coater but have never, in the 20+ years I've been doing this, had any explosive decompose or deflagrate in the coater.
Could be wrong but I think most smokeless powders have a graphite coating, so they may already be conductive.
Hope this helps.
Regards, Andrew T. Werner Chief Metallurgist, Perforating Schlumberger SRC 14910 Airline Road Rosharon, TX 77583
"I Shoot the hippopotamus with bullets made of platinum, because if I use leaden ones his hide is sure to flatten 'em" The Bad Child's Book of Beasts - Hilaire Belloc
-----Original Message----- X-from: hanke-at-mee-inc.com [mailto:hanke-at-mee-inc.com] Sent: Tuesday, April 22, 2014 9:49 AM To: Andrew Werner
Anyone ever examine/analyze gunpowder or primer samples by SEM? Any hints or cautions?
Thanks.
-- ________ Larry D. Hanke, P.E. Principal Engineer Materials Evaluation and Engineering, Inc. (763) 449-8870
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==============================Original Headers============================== 19, 38 -- From werner1-at-slb.com Tue Apr 22 11:15:24 2014 19, 38 -- Received: from gb0135mta01.mail.slb.com (gb0135mta01.mail.slb.com [136.250.9.132]) 19, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MGFOCu024889 19, 38 -- for {microscopy-at-microscopy.com} ; Tue, 22 Apr 2014 11:15:24 -0500 19, 38 -- Received: from gb0135mta01.mail.slb.com (localhost.localdomain [127.0.0.1]) 19, 38 -- by localhost (Postfix) with SMTP id 9B28459D0FB; 19, 38 -- Tue, 22 Apr 2014 16:15:23 +0000 (GMT) 19, 38 -- Received: from NL0230MBX01N1.DIR.slb.com (nl0230mbx01n1.dir.slb.com [199.6.132.5]) 19, 38 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 19, 38 -- (No client certificate requested) 19, 38 -- by gb0135mta01.mail.slb.com (Postfix) with ESMTPS id 6F45C59CF75; 19, 38 -- Tue, 22 Apr 2014 16:15:23 +0000 (GMT) 19, 38 -- Received: from NL0230MBX14N2.DIR.slb.com (199.6.133.31) by 19, 38 -- NL0230MBX01N1.DIR.slb.com (199.6.132.5) with Microsoft SMTP Server (TLS) id 19, 38 -- 14.3.174.1; Tue, 22 Apr 2014 18:15:23 +0200 19, 38 -- Received: from NL0230MBX12N2.DIR.slb.com ([169.254.2.89]) by 19, 38 -- NL0230MBX14N2.DIR.slb.com ([169.254.2.20]) with mapi id 14.03.0174.001; Tue, 19, 38 -- 22 Apr 2014 18:15:23 +0200 19, 38 -- From: Andrew Werner {werner1-at-slb.com} 19, 38 -- To: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} , 19, 38 -- "microscopy-at-microscopy.com" 19, 38 -- {microscopy-at-microscopy.com} 19, 38 -- Subject: RE: [Microscopy] SEM/EDS of Gunpowder 19, 38 -- Thread-Topic: [Microscopy] SEM/EDS of Gunpowder 19, 38 -- Thread-Index: AQHPXjn+UYyVDX2Ys0+cV7Cce5Td+JsdzWmg 19, 38 -- Date: Tue, 22 Apr 2014 16:15:22 +0000 19, 38 -- Message-ID: {72BDC0233CEEDD4AABC3C9463E5EC7AA012902B47C-at-NL0230MBX12N2.DIR.slb.com} 19, 38 -- References: {201404221448.s3MEmZJw016502-at-ns.microscopy.com} 19, 38 -- In-Reply-To: {201404221448.s3MEmZJw016502-at-ns.microscopy.com} 19, 38 -- Accept-Language: en-US 19, 38 -- Content-Language: en-US 19, 38 -- X-MS-Has-Attach: 19, 38 -- X-MS-TNEF-Correlator: 19, 38 -- x-originating-ip: [199.6.133.110] 19, 38 -- Content-Type: text/plain; charset="us-ascii" 19, 38 -- MIME-Version: 1.0 19, 38 -- Content-Transfer-Encoding: 8bit 19, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3MGFOCu024889 ==============================End of - Headers==============================
I have not worked with what the customer called gun powder, but I guess the material had similar properties.
I carried out a good deal of consultancy work with the then Nobel Explosives Division of ICI (they have made explosives since the days of galleons!). The specimens were mounted on a conventional stub using the smallest possible quantity. There was no reason to coat the specimens. I used basic specimen safety measures on a tungsten hairpin sourced instrument- low kV ( {2) lower emission current (30mA) and lower than normal spot sizes (i.e. a spot size suitable for 40,000X when I was working below 10,000X). My safety argument was that the amount of material was very small, and being contained in a vacuum, I thought I was pretty safe.
The client was frightened to carry out the work themselves but were happy with the results I provided for then on several 3 day visits.
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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============================================================================ ===== Anyone ever examine/analyze gunpowder or primer samples by SEM? Any hints or cautions?
Thanks.
-- ________ Larry D. Hanke, P.E. Principal Engineer Materials Evaluation and Engineering, Inc. (763) 449-8870
==============================Original Headers============================== 4, 16 -- From hanke-at-mee-inc.com Tue Apr 22 09:40:33 2014 4, 16 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 4, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MEeXaK004815 4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: (qmail 14315 invoked by uid 507); 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: from c-24-118-160-10.hsd1.mn.comcast.net (HELO ?192.168.1.73?) (24.118.160.10) 4, 16 -- by mail.namisolutions.com with SMTP; 22 Apr 2014 09:40:33 -0500 4, 16 -- Message-ID: {53567F5F.1060302-at-mee-inc.com} 4, 16 -- Date: Tue, 22 Apr 2014 09:40:31 -0500 4, 16 -- From: Larry Hanke {hanke-at-mee-inc.com} 4, 16 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 4, 16 -- MIME-Version: 1.0 4, 16 -- To: microscopy-at-microscopy.com 4, 16 -- Subject: SEM/EDS of Gunpowder 4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 24 -- From protrain-at-emcourses.com Tue Apr 22 11:33:50 2014 19, 24 -- Received: from mail.esentra.net (mail.esentra.net [94.228.36.208]) 19, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MGXoZW011657 19, 24 -- for {microscopy-at-microscopy.com} ; Tue, 22 Apr 2014 11:33:50 -0500 19, 24 -- Received: from mail.clientmail.net (Not Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway 19, 24 -- id {B535699e20001} ; Tue, 22 Apr 2014 17:33:39 +0100 19, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Tue, 22 Apr 2014 17:33:37 +0100 19, 24 -- Reply-To: {protrain-at-emcourses.com} 19, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} 19, 24 -- To: {hanke-at-mee-inc.com} 19, 24 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 19, 24 -- References: {201404221441.s3MEfiSi005878-at-ns.microscopy.com} 19, 24 -- In-Reply-To: {201404221441.s3MEfiSi005878-at-ns.microscopy.com} 19, 24 -- Subject: RE: [Microscopy] SEM/EDS of Gunpowder 19, 24 -- Date: Tue, 22 Apr 2014 17:33:47 +0100 19, 24 -- Organization: Protrain 19, 24 -- Message-ID: {001201cf5e48$a2cc54b0$e864fe10$-at-com} 19, 24 -- MIME-Version: 1.0 19, 24 -- Content-Type: text/plain; 19, 24 -- charset="us-ascii" 19, 24 -- Content-Transfer-Encoding: 7bit 19, 24 -- X-Mailer: Microsoft Office Outlook 12.0 19, 24 -- Thread-Index: Ac9eOPZGi9KPsID2RjyvP9drkML1WAAAHBOw 19, 24 -- Content-Language: en-gb ==============================End of - Headers==============================
From drugstore-canadian8-at-wangjie.net Tue Apr 22 15:27:41 2014 Return-Path: {drugstore-canadian8-at-wangjie.net} Received: from mail1.wangjie.net ([95.72.195.34]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MKRHXI000879 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 22 Apr 2014 15:27:38 -0500 Message-Id: {201404222027.s3MKRHXI000879-at-ns.microscopy.com}
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Apr 23 08:28:11 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3NDSB6M015385 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 23 Apr 2014 08:28:11 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id E0D2E6F0EB4 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 23 Apr 2014 08:28:11 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id GCNnyjpYqP0p for {microscopy-at-microscopy.com} ; 12, 28 -- Wed, 23 Apr 2014 08:28:03 -0500 (CDT) 12, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id 4CAB36F0EA2 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 23 Apr 2014 08:28:03 -0500 (CDT) 12, 28 -- Message-ID: {5357BFE2.2060600-at-microscopy.com} 12, 28 -- Date: Wed, 23 Apr 2014 08:28:02 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:CM10 error noise 12, 28 -- References: {201404230330.s3N3UpUU006280-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201404230330.s3N3UpUU006280-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201404230330.s3N3UpUU006280-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Your supervisor is quite right to warn you of the perils of using Fourier transforms. I've seen some terrible abuse of FFTs, even in the highest impact factor journals where the refereeing should have been better.
I believe your request relates to measuring displacements and rotations of the crystal lattices either side of an interface. Probably the best article I would refer you to is Hytch, Snoeck & Kilaas (Ultramicroscopy 74 (1998) 131—146) who coined the term geometric phase analysis (GPA - not to be confused with geometric phases in quantum mechanics). They explain how to use the the FFT of a HRTEM image to recover information about the displacement, R(x,y), and rotation, omega(x,y), of the lattice from each of the peaks in the FFT. The strains and shears associated with interfaces and steps can be seen as phase ramps. Differentiating these will give local strains and shears.
There are certain caveats to using this technique however, which Martin Hytch & Tobias Plamann address in a later paper (Ultramicroscopy 87 (2001) 199–212). The problem is that, in some crystals, the peaks (and troughs) in a lattice image do not necessarily track the positions of the atomic columns. This occurs if there are a) strong thickness gradients, b) non-centrosymmetric crystals. For both, the local beam tilt (if using a large condenser aperture) and crystal tilts (buckling due to thin-film stresses) lead to the lattice fringes shifting across the unit cell leading to phantom strains (in the case of centrosymmetric crystals the lattice fringes shift by a factor of pi radians and the peaks become troughs and vice versa).
From a practical point of view you need good quality HRTEM images so I would recommend using small condenser apertures and, if possible, energy filtering the image with a Gatan Imaging Filter or in-column filter.
Analysis using GPA can be done either using post-analysis software, e.g. MatLab (I tend to use IDL), or by using a Digital Micrograph script that can be purchased from HREM Research (www.hremresearch.com).*
I hope that helps.
Yours, Jon
Note: I have no links, commercial or otherwise, with Gatan, MatLab, IDL or HREM Research. These are recommendations based on my past experiences.
P.S. If you need a pdf of the articles I refer to, I can send you an electronic copy. Please do ask.
} Title-Subject: [Filtered] High resolution TEM inverse fast Fourier } transformation } } Message: Dear all, } I am a PhD student in material science from Queen's University in } Canada. I am trying to use HRTEM } to characterize the grain boundaries and phase interfaces and } dislocations generated after } deformation in Zr alloy. I have captured some HRTEM images but it is } very hard to interpret them. My } supervisor told me that it should be very careful to explain them. And } as it says in textbook } written by William, we should use simulation software to interpret the } image. However, in open } literature, inverse fast Fourier transformation(IFFT) was widely used } to index the dislocations and } misfits between two phases. And based on my own experience, the IFFT } image is related to the size } and position of the mask which was applied. Could any one give me any } idea about the advantages and } disadvantages of IFFT method to analyze the HRTEM data. My } appreciation for your suggestion and } discussion. } Best Regards } Hongbing Yu
==============================Original Headers============================== 14, 32 -- From jsb43-at-cam.ac.uk Wed Apr 23 08:51:13 2014 14, 32 -- Received: from ppsw-51.csi.cam.ac.uk (ppsw-51.csi.cam.ac.uk [131.111.8.151]) 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3NDpDhC002373 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Apr 2014 08:51:13 -0500 14, 32 -- X-Cam-AntiVirus: no malware found 14, 32 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 14, 32 -- Received: from webmail-2a.csi.cam.ac.uk ([131.111.9.34]:53866) 14, 32 -- by ppsw-51.csi.cam.ac.uk (ppsw.cam.ac.uk [131.111.8.139]:25) 14, 32 -- with esmtp id 1WcxZv-0005yR-Ys (Exim 4.82_3-c0e5623) 14, 32 -- (return-path {jsb43-at-cam.ac.uk} ); Wed, 23 Apr 2014 14:51:11 +0100 14, 32 -- Received: from [127.0.0.1] (helo=webmail-2.hermes.cam.ac.uk) 14, 32 -- by webmail-2a.csi.cam.ac.uk with esmtp id 1WcxZv-000TDX-Nt (Exim 4.80_167-5a66dd3) 14, 32 -- (return-path {jsb43-at-cam.ac.uk} ); Wed, 23 Apr 2014 14:51:11 +0100 14, 32 -- Received: from floater-40-114.msm.private.cam.ac.uk ([172.28.40.114]) 14, 32 -- by webmail-2.hermes.cam.ac.uk 14, 32 -- with HTTP (HTTP/1.1 POST); Wed, 23 Apr 2014 14:51:11 +0100 14, 32 -- MIME-Version: 1.0 14, 32 -- Content-Type: text/plain; charset=UTF-8; 14, 32 -- format=flowed 14, 32 -- X-Authenticated-User: jsb43 14, 32 -- Date: Wed, 23 Apr 2014 14:51:11 +0100 14, 32 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 14, 32 -- To: 12hy1-at-queensu.ca 14, 32 -- Cc: Listserver MSA {Microscopy-at-microscopy.com} 14, 32 -- Subject: Re: [Microscopy] IFFT of HRTEM images 14, 32 -- In-Reply-To: {201404230102.s3N123Ye012540-at-ns.microscopy.com} 14, 32 -- References: {201404230102.s3N123Ye012540-at-ns.microscopy.com} 14, 32 -- Message-ID: {8b2d6d370ae42320957f8679758ee193-at-cam.ac.uk} 14, 32 -- X-Sender: jsb43-at-cam.ac.uk 14, 32 -- User-Agent: Roundcube Webmail/0.9.4 14, 32 -- Content-Transfer-Encoding: 8bit 14, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3NDpDhC002373 ==============================End of - Headers==============================
From meds_popular1-at-bashtel.ru Wed Apr 23 10:10:15 2014 Return-Path: {meds_popular1-at-bashtel.ru} Received: from h178-129-161-115.dyn.bashtel.ru (h178-129-161-115.dyn.bashtel.ru [178.129.161.115]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3NFA9uV023468 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 23 Apr 2014 10:10:11 -0500 Message-Id: {201404231510.s3NFA9uV023468-at-ns.microscopy.com}
I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. Can I convince somebody with my argument?
Stephane
X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} To: nizets2-at-yahoo.com Sent: Tuesday, April 22, 2014 4:44 PM
Anyone ever examine/analyze gunpowder or primer samples by SEM? Any hints or cautions?
Thanks.
-- ________ Larry D. Hanke, P.E. Principal Engineer Materials Evaluation and Engineering, Inc. (763) 449-8870
==============================Original Headers============================== 4, 16 -- From hanke-at-mee-inc.com Tue Apr 22 09:40:33 2014 4, 16 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 4, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MEeXaK004815 4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: (qmail 14315 invoked by uid 507); 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: from c-24-118-160-10.hsd1.mn.comcast.net (HELO ?192.168.1.73?) (24.118.160.10) 4, 16 -- by mail.namisolutions.com with SMTP; 22 Apr 2014 09:40:33 -0500 4, 16 -- Message-ID: {53567F5F.1060302-at-mee-inc.com} 4, 16 -- Date: Tue, 22 Apr 2014 09:40:31 -0500 4, 16 -- From: Larry Hanke {hanke-at-mee-inc.com} 4, 16 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 4, 16 -- MIME-Version: 1.0 4, 16 -- To: microscopy-at-microscopy.com 4, 16 -- Subject: SEM/EDS of Gunpowder 4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Hi Stephane
Yes I 100% agree and you will see that I took this into account in my email. I see no reason to go into fancy techniques when the simplest of procedures will do the job. I did outline those that I had used without a problem during my 6 days of investigations.
I did not carry out EDX and understand that if I had needed to do so I may have required higher than the 2kV that I had used.
Kind regards
---------------------------------------------------------------------------- --------------------------------------- I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. Can I convince somebody with my argument?
Stephane
X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} To: nizets2-at-yahoo.com Sent: Tuesday, April 22, 2014 4:44 PM
Anyone ever examine/analyze gunpowder or primer samples by SEM? Any hints or cautions?
Thanks.
-- ________ Larry D. Hanke, P.E. Principal Engineer Materials Evaluation and Engineering, Inc. (763) 449-8870
==============================Original Headers============================== 4, 16 -- From hanke-at-mee-inc.com Tue Apr 22 09:40:33 2014 4, 16 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 4, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MEeXaK004815 4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: (qmail 14315 invoked by uid 507); 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: from c-24-118-160-10.hsd1.mn.comcast.net (HELO ?192.168.1.73?) (24.118.160.10) 4, 16 -- by mail.namisolutions.com with SMTP; 22 Apr 2014 09:40:33 -0500 4, 16 -- Message-ID: {53567F5F.1060302-at-mee-inc.com} 4, 16 -- Date: Tue, 22 Apr 2014 09:40:31 -0500 4, 16 -- From: Larry Hanke {hanke-at-mee-inc.com} 4, 16 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 4, 16 -- MIME-Version: 1.0 4, 16 -- To: microscopy-at-microscopy.com 4, 16 -- Subject: SEM/EDS of Gunpowder 4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I believe that most explosives contain their own oxidizers to enable the rapid reaction. Hence the presence of peroxide in many of the homemade concoctions. Anyone want to clarify the issue?
Cheers, Henk
On 4/24/2014 7:35 AM, nizets2-at-yahoo.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. } And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. } Can I convince somebody with my argument? } } Stephane } } } } X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} } To: nizets2-at-yahoo.com } Sent: Tuesday, April 22, 2014 4:44 PM } Subject: [Microscopy] SEM/EDS of Gunpowder } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Anyone ever examine/analyze gunpowder or primer samples by } SEM? Any hints or cautions? } } Thanks. }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
That is my understanding as well. In an explosion, there is not enough time to rely upon mass transfer of oxygen to fuel the reaction. The reaction is a rearrangement of atoms already present.
The question will be what energy threshold needs to be exceeded to start the reaction. Low voltages and beam currents are a good approach. High explosives may be safer because they need quite a kick from a detonator to get started.
Of course, it is also helpful to limit the material examined so if something goes off it won't damage the instrument.
I'd be interested if someone could address those issues.
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Thursday, April 24, 2014 7:16 AM
Hi Stephane,
I believe that most explosives contain their own oxidizers to enable the rapid reaction. Hence the presence of peroxide in many of the homemade concoctions. Anyone want to clarify the issue?
Cheers, Henk
On 4/24/2014 7:35 AM, nizets2-at-yahoo.com wrote: } } } I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. } And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. } Can I convince somebody with my argument? } } Stephane } } X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} } To: nizets2-at-yahoo.com } Sent: Tuesday, April 22, 2014 4:44 PM } Subject: [Microscopy] SEM/EDS of Gunpowder } } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Anyone ever examine/analyze gunpowder or primer samples by SEM? Any } hints or cautions? } } Thanks. }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
Combustion does require fuel and oxidizer. However, explosives and propellants such as smokeless powder are molecules containing hydrogen, carbon, nitrogen, and oxygen in a relatively stable configuration, that can be disturbed by energy input (shock, heat etc.) an decompose into carbon dioxide / monoxide, water vapor, and nitrogen.
That is, these compounds contain both fuel and oxidizer, and the explosion is essentially the reconfiguration of the components into more stable compounds, with a concomitant release of energy (heat and noise).
If you google TNT you will find that it is a 6 carbon benzene ring with one hydrogen, one CH3 group, and three NO2 groups. The reaction can be written (please excuse lack of subscripts):
C7H5N2O6 ---} 1.5 N2 + 2.5 H2O + 3.5 CO + 3.5 C. If it had a little more oxygen the last CO and C would be fully oxidized to CO2.
Anyway, it takes a "spark" - some unstabilizing input - to kick off the reaction. And since this has little to do with microscopy I'll shut up now.
Regards, Andrew
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, April 24, 2014 6:43 AM To: Andrew Werner
I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. Can I convince somebody with my argument?
Stephane
X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} To: nizets2-at-yahoo.com Sent: Tuesday, April 22, 2014 4:44 PM
Anyone ever examine/analyze gunpowder or primer samples by SEM? Any hints or cautions?
Thanks.
-- ________ Larry D. Hanke, P.E. Principal Engineer Materials Evaluation and Engineering, Inc. (763) 449-8870
==============================Original Headers============================== 4, 16 -- From hanke-at-mee-inc.com Tue Apr 22 09:40:33 2014 4, 16 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 4, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MEeXaK004815 4, 16 -- for {microscopy-at-microscopy.com} ; Tue, 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: (qmail 14315 invoked by uid 507); 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: from c-24-118-160-10.hsd1.mn.comcast.net (HELO ?192.168.1.73?) (24.118.160.10) 4, 16 -- by mail.namisolutions.com with SMTP; 22 Apr 2014 09:40:33 -0500 4, 16 -- Message-ID: {53567F5F.1060302-at-mee-inc.com} 4, 16 -- Date: Tue, 22 Apr 2014 09:40:31 -0500 4, 16 -- From: Larry Hanke {hanke-at-mee-inc.com} 4, 16 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 4, 16 -- MIME-Version: 1.0 4, 16 -- To: microscopy-at-microscopy.com 4, 16 -- Subject: SEM/EDS of Gunpowder 4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I've never been afraid of explosive damage, but the idea of releasing those potentially corrosive gases and moisture in my SEM has always given me the heebie-jeebies (surprisingly, the spell checker has the correct version of heebie-jeebies... but it didn't have microtome go figure.)
Stay safe......... Frank
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Thursday, April 24, 2014 9:54 AM To: Frank Karl
That is my understanding as well. In an explosion, there is not enough time to rely upon mass transfer of oxygen to fuel the reaction. The reaction is a rearrangement of atoms already present.
The question will be what energy threshold needs to be exceeded to start the reaction. Low voltages and beam currents are a good approach. High explosives may be safer because they need quite a kick from a detonator to get started.
Of course, it is also helpful to limit the material examined so if something goes off it won't damage the instrument.
I'd be interested if someone could address those issues.
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Thursday, April 24, 2014 7:16 AM
Hi Stephane,
I believe that most explosives contain their own oxidizers to enable the rapid reaction. Hence the presence of peroxide in many of the homemade concoctions. Anyone want to clarify the issue?
Cheers, Henk
On 4/24/2014 7:35 AM, nizets2-at-yahoo.com wrote: } } } I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. } And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. } Can I convince somebody with my argument? } } Stephane } } X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} } To: nizets2-at-yahoo.com } Sent: Tuesday, April 22, 2014 4:44 PM } Subject: [Microscopy] SEM/EDS of Gunpowder } } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Anyone ever examine/analyze gunpowder or primer samples by SEM? Any } hints or cautions? } } Thanks. }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
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==============================Original Headers============================== 34, 31 -- From frank_karl-at-ardl.com Thu Apr 24 09:08:07 2014 34, 31 -- Received: from cal1-mh744b.smtproutes.com (cal1-mh744b.smtproutes.com [208.70.89.150]) 34, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3OE87cg013613 34, 31 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 09:08:07 -0500 34, 31 -- X-Katharion-ID: 1398348457.75079.cal1-mh744 34, 31 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by 34, 31 -- cal1-mh744.smtproutes.com [(192.69.16.66)] with ESMTP via TCP 34, 31 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 24 Apr 2014 14:07:37 +0000 34, 31 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 34, 31 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Thu, 24 34, 31 -- Apr 2014 10:07:36 -0400 34, 31 -- From: Frank Karl {frank_karl-at-ardl.com} 34, 31 -- To: "'wesaia-at-iastate.edu'" {wesaia-at-iastate.edu} 34, 31 -- CC: "Microscopy Listserver (microscopy-at-microscopy.com)" 34, 31 -- {microscopy-at-microscopy.com} 34, 31 -- Date: Thu, 24 Apr 2014 10:07:36 -0400 34, 31 -- Subject: RE: [Microscopy] Fw: SEM/EDS of Gunpowder 34, 31 -- Thread-Topic: [Microscopy] Fw: SEM/EDS of Gunpowder 34, 31 -- Thread-Index: Ac9fxMERgoraukMZQweqzJsdt4D7nQAASWaw 34, 31 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BF33A0696-at-exchange2k7.ad.ardl.com} 34, 31 -- References: {201404241354.s3ODs87R025314-at-ns.microscopy.com} 34, 31 -- In-Reply-To: {201404241354.s3ODs87R025314-at-ns.microscopy.com} 34, 31 -- Accept-Language: en-US 34, 31 -- Content-Language: en-US 34, 31 -- X-MS-Has-Attach: 34, 31 -- X-MS-TNEF-Correlator: 34, 31 -- acceptlanguage: en-US 34, 31 -- Content-Type: text/plain; charset="us-ascii" 34, 31 -- MIME-Version: 1.0 34, 31 -- Content-Transfer-Encoding: 8bit 34, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3OE87cg013613 ==============================End of - Headers==============================
Gunpowder ordinarily consists of three components-potassium nitrate, also called saltpeter (KNO3), charcoal (C), and sulfur (S). Proportions of these components may vary, and typically are 65 to 75 percent potassium nitrate, 15 to 20 percent charcoal, and 10 to 15 percent sulfur.
Interestingly, stoichiometrically,1 the reaction proportions are 84 to 8 to 8. The reaction is considered to generally be,
10 KNO3 + 8 C + 3 S → 2 K2CO3 + 3 K2SO4 + 6 CO2↑ + 5 N2↑
Gunpowder - What Produces the Bang?
Most chemical reactions do not include a bang. Why does the ignition of gunpowder-after all, simply a chemical reaction-do so? It is because of the sudden generation of pressure within an enclosed space. Gunpowder in a closed tube creates enough pressure it violently shatters the container (perhaps made of steel) and generates a "shock wave."2 How?
The chemical reactants-all solid-do not take up much volume. On the other hand, the products generated include 11 parts of carbon dioxide and nitrogen-gases. Now the solid products are slightly less in volume than the solid reactants, so it might seem the pressure should be decreasing, not increasing. However, the volume of the gas products immensely greater, since the atoms in a gas are very far apart. It is the sudden conversion of a small amount of solid material into gas that produces tremendous pressure--suddenly bursting the container-that produces the characteristic explosion with its associated Bang! Anyone who has observed a fireworks display knows that at the end of a beautiful aerial flower his ears may be assaulted by an occasional series of sharp Bangs. Bang-bang-bang-bang-bang-BANG!
Regards, Steve
Steve Buckingham Surface Scientist Surface Science ____________________________________________________________________
Novelis Global Research & Technology Center 1950 Vaughn Road Kennesaw, GA 30144
Cell:Â Â Â 770-547-3381 Office: 770-795-6767 steve.buckingham-at-novelis.com www.novelis.com
Not just aluminum, Novelis Aluminum.
-----Original Message----- X-from: werner1-at-slb.com [mailto:werner1-at-slb.com] Sent: Thursday, April 24, 2014 10:09 AM To: Stephen Buckingham
Stephane,
Combustion does require fuel and oxidizer. However, explosives and propellants such as smokeless powder are molecules containing hydrogen, carbon, nitrogen, and oxygen in a relatively stable configuration, that can be disturbed by energy input (shock, heat etc.) an decompose into carbon dioxide / monoxide, water vapor, and nitrogen.
That is, these compounds contain both fuel and oxidizer, and the explosion is essentially the reconfiguration of the components into more stable compounds, with a concomitant release of energy (heat and noise).
If you google TNT you will find that it is a 6 carbon benzene ring with one hydrogen, one CH3 group, and three NO2 groups. The reaction can be written (please excuse lack of subscripts):
C7H5N2O6 ---} 1.5 N2 + 2.5 H2O + 3.5 CO + 3.5 C. If it had a little more oxygen the last CO and C would be fully oxidized to CO2.
Anyway, it takes a "spark" - some unstabilizing input - to kick off the reaction. And since this has little to do with microscopy I'll shut up now.
Regards, Andrew
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, April 24, 2014 6:43 AM To: Andrew Werner
I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. Can I convince somebody with my argument?  Stephane  Â
X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} To: nizets2-at-yahoo.com Sent: Tuesday, April 22, 2014 4:44 PM
Anyone ever examine/analyze gunpowder or primer samples by SEM? Any hints or cautions?
Thanks.
-- ________ Larry D. Hanke, P.E. Principal Engineer Materials Evaluation and Engineering, Inc. (763) 449-8870
==============================Original Headers============================== 4, 16 -- From hanke-at-mee-inc.com Tue Apr 22 09:40:33 2014 4, 16 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 4, 16 -- Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MEeXaK004815 4, 16 -- Â Â Â for {microscopy-at-microscopy.com} ; Tue, 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: (qmail 14315 invoked by uid 507); 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: from c-24-118-160-10.hsd1.mn.comcast.net (HELO ?192.168.1.73?) (24.118.160.10) 4, 16 --Â by mail.namisolutions.com with SMTP; 22 Apr 2014 09:40:33 -0500 4, 16 -- Message-ID: {53567F5F.1060302-at-mee-inc.com} 4, 16 -- Date: Tue, 22 Apr 2014 09:40:31 -0500 4, 16 -- From: Larry Hanke {hanke-at-mee-inc.com} 4, 16 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 4, 16 -- MIME-Version: 1.0 4, 16 -- To: microscopy-at-microscopy.com 4, 16 -- Subject: SEM/EDS of Gunpowder 4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Well, I said I'd shut up but a couple of additional points:
You are right, no time for mass transfer, the explosive molecule is broken down and rearranged into smaller, simpler molecules with the release of heat.
The energy threshold is sticky - it is why we have rules about cutting detonating cord with a very sharp blade and not "sawing" on it - friction can set it off, but it has to be enough, and concentrated/localized.
High explosives can go "low order" - they can deflagrate instead of detonate. High order detonation means the reaction front moves faster than the sound speed in the material; low order means the shock wave (sound) can outrun the reaction. High order puts the energy out faster/sooner. But high explosives aren't necessarily harder to initiate than low.
You are absolutely right about limiting the amount of energetic material, for a couple of reasons. One it total energy involved - deflagration of a few milligrams will contaminate the vacuum system but not damage the microscope chamber. The other is what is called "run up to detonation" and (roughly), works like - starting one spot burning, the reaction is relatively slow, but picks up speed as heat accumulates and finally gets fast enough to outrun the sound wave - it goes high order. Limiting the amount of material obviates this possibility.
Look, I'm a Metallurgist who works with explosives people and happens to use microscopes - not really a Microscopist or Shock Physicist or Chemical Engineer. But explosives are fascinating and useful - we use a lot more in mining and oilfield than they do in military applications every year. So, if anyone is interested in them, get Paul Cooper's book (with Stan Kurowski) Introduction to the Technology of Explosives - ISBN 0-471-18635-X - it is very accessible and fascinating.
Regards, Andrew
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Thursday, April 24, 2014 8:53 AM To: Andrew Werner
That is my understanding as well. In an explosion, there is not enough time to rely upon mass transfer of oxygen to fuel the reaction. The reaction is a rearrangement of atoms already present.
The question will be what energy threshold needs to be exceeded to start the reaction. Low voltages and beam currents are a good approach. High explosives may be safer because they need quite a kick from a detonator to get started.
Of course, it is also helpful to limit the material examined so if something goes off it won't damage the instrument.
I'd be interested if someone could address those issues.
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Thursday, April 24, 2014 7:16 AM
Hi Stephane,
I believe that most explosives contain their own oxidizers to enable the rapid reaction. Hence the presence of peroxide in many of the homemade concoctions. Anyone want to clarify the issue?
Cheers, Henk
On 4/24/2014 7:35 AM, nizets2-at-yahoo.com wrote: } } } I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. } And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. } Can I convince somebody with my argument? } } Stephane } } X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} } To: nizets2-at-yahoo.com } Sent: Tuesday, April 22, 2014 4:44 PM } Subject: [Microscopy] SEM/EDS of Gunpowder } } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Anyone ever examine/analyze gunpowder or primer samples by SEM? Any } hints or cautions? } } Thanks. }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
colijn.1-at-osu.edu wrote: } } } I believe that most explosives contain their own oxidizers to enable the } rapid reaction. Hence the presence of peroxide in many of the homemade } concoctions. Anyone want to clarify the issue? } }
A gunpowder explosion is a deflagration (rapid burning, yes, but with the oxidizer in the composition) instead of a detonation, which is what defines "high explosives". If you google those two terms, you'll get a complete explanation.
Exactly! This is what is known these days as "black powder" and was originally known as gunpowder before the advent of smokeless powder. Black powder is an intimate mixture of the three components, mixed, dampened, granulated, and dried.
Modern small arms propellants (smokeless powder) are generally nitrocellulose based (there are single base and double base powders, the double base contain some nitroglycerine), granulated, and coated with deterrent coatings that encourage progressive burning - controlled deflagration - to tailor the pressure-time curve to the projectile/barrel length combination at hand.
You can make nitrocellulose easily by nitrating cotton or paper with nitric and sulfuric acids. Note that this is something to do in controlled conditions, with proper cooling and safeguards - but my 7th grade Science Teacher, Miss Wyle, showed us and actually may be the person ultimately responsible for the course my life has taken (she was great).
Anyway, I wish it were Friday and I could continue this, but I've got to get back to work.
Regards, Andrew
-----Original Message----- X-from: Stephen Buckingham [mailto:steve.buckingham-at-novelis.com] Sent: Thursday, April 24, 2014 9:11 AM To: Andrew Werner Cc: microscopy-at-microscopy.com
Stephane,
Combustion does require fuel and oxidizer. However, explosives and propellants such as smokeless powder are molecules containing hydrogen, carbon, nitrogen, and oxygen in a relatively stable configuration, that can be disturbed by energy input (shock, heat etc.) an decompose into carbon dioxide / monoxide, water vapor, and nitrogen.
That is, these compounds contain both fuel and oxidizer, and the explosion is essentially the reconfiguration of the components into more stable compounds, with a concomitant release of energy (heat and noise).
If you google TNT you will find that it is a 6 carbon benzene ring with one hydrogen, one CH3 group, and three NO2 groups. The reaction can be written (please excuse lack of subscripts):
C7H5N2O6 ---} 1.5 N2 + 2.5 H2O + 3.5 CO + 3.5 C. If it had a little more oxygen the last CO and C would be fully oxidized to CO2.
Anyway, it takes a "spark" - some unstabilizing input - to kick off the reaction. And since this has little to do with microscopy I'll shut up now.
Regards, Andrew
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, April 24, 2014 6:43 AM To: Andrew Werner
I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. Can I convince somebody with my argument?  Stephane  Â
X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} To: nizets2-at-yahoo.com Sent: Tuesday, April 22, 2014 4:44 PM
Anyone ever examine/analyze gunpowder or primer samples by SEM? Any hints or cautions?
Thanks.
-- ________ Larry D. Hanke, P.E. Principal Engineer Materials Evaluation and Engineering, Inc. (763) 449-8870
==============================Original Headers============================== 4, 16 -- From hanke-at-mee-inc.com Tue Apr 22 09:40:33 2014 4, 16 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 4, 16 -- Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3MEeXaK004815 4, 16 -- Â Â Â for {microscopy-at-microscopy.com} ; Tue, 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: (qmail 14315 invoked by uid 507); 22 Apr 2014 09:40:33 -0500 4, 16 -- Received: from c-24-118-160-10.hsd1.mn.comcast.net (HELO ?192.168.1.73?) (24.118.160.10) 4, 16 --Â by mail.namisolutions.com with SMTP; 22 Apr 2014 09:40:33 -0500 4, 16 -- Message-ID: {53567F5F.1060302-at-mee-inc.com} 4, 16 -- Date: Tue, 22 Apr 2014 09:40:31 -0500 4, 16 -- From: Larry Hanke {hanke-at-mee-inc.com} 4, 16 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 4, 16 -- MIME-Version: 1.0 4, 16 -- To: microscopy-at-microscopy.com 4, 16 -- Subject: SEM/EDS of Gunpowder 4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
werner1-at-slb.com wrote: } } } Modern small arms propellants (smokeless powder) are generally nitrocellulose based (there are single base and double base powders, the double base contain some nitroglycerine), granulated, and coated with deterrent coatings that encourage progressive burning - controlled deflagration - to tailor the pressure-time curve to the projectile/barrel length combination at hand. } } You can make nitrocellulose easily by nitrating cotton or paper with nitric and sulfuric acids. Note that this is something to do in controlled conditions, with proper cooling and safeguards - but my 7th grade Science Teacher, Miss Wyle, showed us and actually may be the person ultimately responsible for the course my life has taken (she was great). } }
Right, sorry, I was thinking about black powder, not modern smokeless powder which is what "gunpowder" currently means. Andrew was typing his more detailed explanations while I was still sending mine.
In relation to the comments about column contamination, the fumes from the "explosive" instruments pump were disgusting. A column clean, new filters, and fresh fluids in the pumps, as expected, made no difference. This column was in real trouble after years of working with explosives.
The best solution we could use to reduce "operator" contamination was to dump the RP outlet to the outside world; sorry greens!
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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-----Original Message----- X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com] Sent: 24 April 2014 15:09 To: protrain-at-emcourses.com
That is my understanding as well. In an explosion, there is not enough time to rely upon mass transfer of oxygen to fuel the reaction. The reaction is a rearrangement of atoms already present.
The question will be what energy threshold needs to be exceeded to start the reaction. Low voltages and beam currents are a good approach. High explosives may be safer because they need quite a kick from a detonator to get started.
Of course, it is also helpful to limit the material examined so if something goes off it won't damage the instrument.
I'd be interested if someone could address those issues.
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Thursday, April 24, 2014 7:16 AM
Hi Stephane,
I believe that most explosives contain their own oxidizers to enable the rapid reaction. Hence the presence of peroxide in many of the homemade concoctions. Anyone want to clarify the issue?
Cheers, Henk
On 4/24/2014 7:35 AM, nizets2-at-yahoo.com wrote: } } } I would be happy to be corrected if I was wrong but I always thought that an explosion was a very fast combustion. } And that a combustion needs oxygen, which is by definition absent from a high vacuum environment. } Can I convince somebody with my argument? } } Stephane } } X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com} } To: nizets2-at-yahoo.com } Sent: Tuesday, April 22, 2014 4:44 PM } Subject: [Microscopy] SEM/EDS of Gunpowder } } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Anyone ever examine/analyze gunpowder or primer samples by SEM? Any } hints or cautions? } } Thanks. }
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==============================Original Headers============================== 34, 31 -- From frank_karl-at-ardl.com Thu Apr 24 09:08:07 2014 34, 31 -- Received: from cal1-mh744b.smtproutes.com (cal1-mh744b.smtproutes.com [208.70.89.150]) 34, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3OE87cg013613 34, 31 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 09:08:07 -0500 34, 31 -- X-Katharion-ID: 1398348457.75079.cal1-mh744 34, 31 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by 34, 31 -- cal1-mh744.smtproutes.com [(192.69.16.66)] with ESMTP via TCP 34, 31 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 24 Apr 2014 14:07:37 +0000 34, 31 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 34, 31 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Thu, 24 34, 31 -- Apr 2014 10:07:36 -0400 34, 31 -- From: Frank Karl {frank_karl-at-ardl.com} 34, 31 -- To: "'wesaia-at-iastate.edu'" {wesaia-at-iastate.edu} 34, 31 -- CC: "Microscopy Listserver (microscopy-at-microscopy.com)" 34, 31 -- {microscopy-at-microscopy.com} 34, 31 -- Date: Thu, 24 Apr 2014 10:07:36 -0400 34, 31 -- Subject: RE: [Microscopy] Fw: SEM/EDS of Gunpowder 34, 31 -- Thread-Topic: [Microscopy] Fw: SEM/EDS of Gunpowder 34, 31 -- Thread-Index: Ac9fxMERgoraukMZQweqzJsdt4D7nQAASWaw 34, 31 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BF33A0696-at-exchange2k7.ad.ardl.com} 34, 31 -- References: {201404241354.s3ODs87R025314-at-ns.microscopy.com} 34, 31 -- In-Reply-To: {201404241354.s3ODs87R025314-at-ns.microscopy.com} 34, 31 -- Accept-Language: en-US 34, 31 -- Content-Language: en-US 34, 31 -- X-MS-Has-Attach: 34, 31 -- X-MS-TNEF-Correlator: 34, 31 -- acceptlanguage: en-US 34, 31 -- Content-Type: text/plain; charset="us-ascii" 34, 31 -- MIME-Version: 1.0 34, 31 -- Content-Transfer-Encoding: 8bit 34, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3OE87cg013613 ==============================End of - Headers==============================
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Title-Subject: [Filtered] Ultrastructural pathology : Unknown object in muscle fiber
Message: Hi,
We found some electron dense material in the skeletal muscle fiber at subsarcolemmal as well as intermyofibrillar spaces. It can be seen in mitochondria and even in endomysium also. Higher magnification show it is not filamentous inclusion. Then what it could be.?
Clue : Skeletal muscle biopsy from 6 years male, Histopathology suggests Conginetal muscular dystrophy.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Apr 24 12:41:28 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3OHfSoL025983 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 12:41:28 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id DBF976F1C02 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 12:41:28 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id WCb9qEuGOohB for {microscopy-at-microscopy.com} ; 12, 28 -- Thu, 24 Apr 2014 12:41:25 -0500 (CDT) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id 8EDE76F1BF0 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 12:41:25 -0500 (CDT) 12, 28 -- Message-ID: {53594CC5.5010808-at-microscopy.com} 12, 28 -- Date: Thu, 24 Apr 2014 12:41:25 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Ultrastructural pathology : Unknown object in muscle fiber 12, 28 -- References: {201404241547.s3OFllMn003236-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201404241547.s3OFllMn003236-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201404241547.s3OFllMn003236-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
have just had a very short view of your images on FLICKR.com (hopefully I have seen all).
My impressions so far(not to teach you!):
i) ultrastructural preservation seems to be compromised, not knowing whether due to delay in fixation, incorrect fixative [regarding mosmol, pH and / or composition] or really (true) caused by the / a disease. ii) most, if not all mitochondria contained in the images show swelling and dysmorphic mitochondrial texture (enlarged, empty mitoch. matrix, only few remnants of cristae mitoch.) [causes: as before] iii) most of the "dark, e-dense material" suggested to be products of mitochondrial degeneration, since usually within the former mitochondrial membrane(s). iv) the cylindric lamellar structure in Tv32 IMHO is a degenerated mitochondrium (with a central, so called "glycogen body", said to be end-product of mitochondrial degeneration - but this never seen to be scientifically confirmed), but perhaps could be assigned also to SER/t-tubulus canal. The e-dense, round area could be {morphological equivalent} of mitochondrial energy supply (e. g./i.e. [phosphor-]lipidic material normally to be transferred into the mitochondrium via its membrane, but in the condition shown, disturbed due to mitochondrial degeneration (vice verse, means: primarily or secondarily disturbed transfer/transport mechanism). This circumstance / possibility is more detailed and can be seen in Tv10 & Tv9, which reminds me on a (juvenile muscle biopsy) case with ATP-synthase or another enzyme deficiency. v) if the e-dense material would be "normal" (neutral) lipids in the specimen, as seen with / after classical TEM-spec. processing, such would have been eliminated (empty vacuoles). Therefore it would be a good idea to tell us a little bit more about your routine/special processing of the biopsy.
Hope this helps anyway, unfortunately in a hurry for special reason, so I cannot comment on possible entities or similar cases/morphologies.... perhaps I will return to the thread tomorrow...
Best wishes and regards Wolfgang
Wolfgang MUSS EM-Lab Univ.Inst. Pathology SALK-LKH (Gen. Hosp.) and PMU SALZBURG SALZBURG, AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: microscopylistserver-noreply-at-microscopy.com } [mailto:microscopylistserver-noreply-at-microscopy.com] } Gesendet: Donnerstag, 24. April 2014 19:46 } An: Muß Wolfgang } Betreff: [Microscopy] Ultrastructural pathology : Unknown object in } muscle fiber } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } X-from: ravi.thakkar369-at-gmail.com () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both ravi.thakkar369-at-gmail.com as well as the } Microscopy Listserver } ----------------------------------------------------------------------- } ---- } } Email: ravi.thakkar369-at-gmail.com } Name: Ravi Thakkar } } Title-Subject: [Filtered] Ultrastructural pathology : Unknown object in } muscle fiber } } Message: Hi, } } We found some electron dense material in the skeletal muscle fiber at } subsarcolemmal as well as intermyofibrillar spaces. } It can be seen in mitochondria and even in endomysium also. Higher } magnification show it is not filamentous inclusion. } Then what it could be.? } } Clue : Skeletal muscle biopsy from 6 years male, Histopathology } suggests Conginetal muscular dystrophy. } } Images : https://www.flickr.com/photos/97321550-at-N08/13991844032/ } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original } Headers============================== } 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Apr 24 } 12:41:28 2014 } 12, 28 -- Received: from znl.com ([206.69.208.20]) } 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s3OHfSoL025983 } 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 12:41:28 } -0500 } 12, 28 -- Received: from localhost (localhost [127.0.0.1]) } 12, 28 -- by znl.com (Postfix) with ESMTP id DBF976F1C02 } 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 12:41:28 } -0500 (CDT) } 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 28 -- Received: from znl.com ([127.0.0.1]) } 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd- } new, port 10024) } 12, 28 -- with ESMTP id WCb9qEuGOohB for {microscopy-at-microscopy.com} ; } 12, 28 -- Thu, 24 Apr 2014 12:41:25 -0500 (CDT) } 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com } [206.69.208.22]) } 12, 28 -- by znl.com (Postfix) with ESMTPA id 8EDE76F1BF0 } 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 12:41:25 } -0500 (CDT) } 12, 28 -- Message-ID: {53594CC5.5010808-at-microscopy.com} } 12, 28 -- Date: Thu, 24 Apr 2014 12:41:25 -0500 } 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver- } noreply-at-microscopy.com} } 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; } rv:24.0) Gecko/20100101 Thunderbird/24.4.0 } 12, 28 -- MIME-Version: 1.0 } 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 28 -- Subject: viaWWW:Ultrastructural pathology : Unknown object in } muscle fiber } 12, 28 -- References: {201404241547.s3OFllMn003236-at-ns.microscopy.com} } 12, 28 -- In-Reply-To: {201404241547.s3OFllMn003236-at-ns.microscopy.com} } 12, 28 -- X-Forwarded-Message-Id: } {201404241547.s3OFllMn003236-at-ns.microscopy.com} } 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 28 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 40 -- From W.Muss-at-salk.at Thu Apr 24 13:20:27 2014 9, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 9, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3OIKRvW012967 9, 40 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 13:20:27 -0500 9, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 9, 40 -- by hermes.salk.at (Postfix) with ESMTP id C3A30646B48 9, 40 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 20:20:25 +0200 (CEST) 9, 40 -- X-Scanned-By: SALK-Content-Filter 9, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 9, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 9, 40 -- with ESMTP id qEiRoMQ6prnY for {microscopy-at-microscopy.com} ; 9, 40 -- Thu, 24 Apr 2014 20:20:25 +0200 (CEST) 9, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 9, 40 -- by hermes.salk.at (Postfix) with ESMTP id 9DCD5646B5D 9, 40 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 20:20:25 +0200 (CEST) 9, 40 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 9, 40 -- by N1EX198.lks.local with ESMTP; 24 Apr 2014 20:25:53 +0200 9, 40 -- Received: from N1DT132.lksdom21.lks.local ([fe80::c9bf:82a7:c92e:ef25]) by 9, 40 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 9, 40 -- 14.02.0387.000; Thu, 24 Apr 2014 20:20:25 +0200 9, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 9, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 40 -- Subject: [Microscopy] Re: Ultrastructural pathology : Unknown object in 9, 40 -- muscle fiber 9, 40 -- Thread-Topic: [Microscopy] Re: Ultrastructural pathology : Unknown object in 9, 40 -- muscle fiber 9, 40 -- Thread-Index: AQHPX+cnSC/zRTVz7kONCqSoUKxP1g== 9, 40 -- Date: Thu, 24 Apr 2014 18:20:24 +0000 9, 40 -- Message-ID: {3D22E6EE65F39E448DCE416281537671207D331B-at-N1DT132.lksdom21.lks.local} 9, 40 -- References: {201404241746.s3OHk1ef030359-at-ns.microscopy.com} 9, 40 -- In-Reply-To: {201404241746.s3OHk1ef030359-at-ns.microscopy.com} 9, 40 -- Accept-Language: de-DE, en-US 9, 40 -- Content-Language: de-DE 9, 40 -- X-MS-Has-Attach: 9, 40 -- X-MS-TNEF-Correlator: 9, 40 -- x-originating-ip: [192.168.42.2] 9, 40 -- Content-Type: text/plain; charset="iso-8859-1" 9, 40 -- MIME-Version: 1.0 9, 40 -- Content-Transfer-Encoding: 8bit 9, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3OIKRvW012967 ==============================End of - Headers==============================
So is the fear that the SEM Vacuum chamber cam blow up since the gun powder can fuel its own combustion? So does this mean current guns and ammo technology will still be good weapons to use in outer space? } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } werner1-at-slb.com wrote: } } } } } } Modern small arms propellants (smokeless powder) are generally nitrocellulose based (there are single base and double base powders, the double base contain some nitroglycerine), granulated, and coated with deterrent coatings that encourage progressive burning - controlled deflagration - to tailor the pressure-time curve to the projectile/barrel length combination at hand. } } } } You can make nitrocellulose easily by nitrating cotton or paper with nitric and sulfuric acids. Note that this is something to do in controlled conditions, with proper cooling and safeguards - but my 7th grade Science Teacher, Miss Wyle, showed us and actually may be the person ultimately responsible for the course my life has taken (she was great). } } } } } } Right, sorry, I was thinking about black powder, not modern } smokeless powder which is what "gunpowder" currently means. } Andrew was typing his more detailed explanations while I was } still sending mine. } } This is a bit OT, but: } } } http://explosives.mst.edu/media/academic/explosives/documents/camp/2014ExplosivesCamp.pdf } } if you have a kid 16 or older (or if you're young enough to pass } for a high-school kid, which definitely excludes me), check this out! } } Rick Mott (guilty of making NI3 and other noisy fun stuff as } a teenager) } } ==============================Original Headers============================== } 5, 18 -- From rmott-at-pulsetor.com Thu Apr 24 10:05:42 2014 } 5, 18 -- Received: from p3plsmtpa08-05.prod.phx3.secureserver.net (p3plsmtpa08-05.prod.phx3.secureserver.net [173.201.193.106]) } 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3OF5gbY016131 } 5, 18 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 10:05:42 -0500 } 5, 18 -- Received: from [127.0.0.1] ([192.54.222.11]) } 5, 18 -- by p3plsmtpa08-05.prod.phx3.secureserver.net with } 5, 18 -- id tr5b1n0010FMozk01r5e1M; Thu, 24 Apr 2014 08:05:38 -0700 } 5, 18 -- Message-ID: {53592836.7030100-at-pulsetor.com} } 5, 18 -- Date: Thu, 24 Apr 2014 11:05:26 -0400 } 5, 18 -- From: Rick Mott {rmott-at-pulsetor.com} } 5, 18 -- User-Agent: Thunderbird 2.0.0.24 (Windows/20100228) } 5, 18 -- MIME-Version: 1.0 } 5, 18 -- To: microscopy-at-microscopy.com } 5, 18 -- Subject: Re: [Microscopy] Fw: SEM/EDS of Gunpowder } 5, 18 -- References: {201404241445.s3OEjBij000870-at-ns.microscopy.com} } 5, 18 -- In-Reply-To: {201404241445.s3OEjBij000870-at-ns.microscopy.com} } 5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 18 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
I think the concern is that the energetic material could potentially decompose into it's reaction products, liberating some heat and gas - CO2, H2O, N2, CO - that might be a pain for the vacuum system to deal with.
But yes, current guns and ammunition will work in vacuum. You would need less volatile lubricants on moving parts, or solid-state anti-friction coatings (hard chrome or spray moly might work), and the barrel might be subject to excessive copper fouling - the bullet jacket riding directly on the lands might gall - but we moly coat match bullets now, so I can't immediately think of any insurmountable difficulties. Except - you need a good backstop (here on Earth a substantial dirt berm works well), and in the absence of a significant gravitational field, recoil will send the shooter tumbling / spinning. And - with no wind to read and compensate, where is the challenge?
Andrew
-----Original Message----- X-from: mmilewski-at-comcast.net [mailto:mmilewski-at-comcast.net] Sent: Thursday, April 24, 2014 3:21 PM To: Andrew Werner
So is the fear that the SEM Vacuum chamber cam blow up since the gun powder can fuel its own combustion? So does this mean current guns and ammo technology will still be good weapons to use in outer space? } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } werner1-at-slb.com wrote: } } } } } } Modern small arms propellants (smokeless powder) are generally nitrocellulose based (there are single base and double base powders, the double base contain some nitroglycerine), granulated, and coated with deterrent coatings that encourage progressive burning - controlled deflagration - to tailor the pressure-time curve to the projectile/barrel length combination at hand. } } } } You can make nitrocellulose easily by nitrating cotton or paper with nitric and sulfuric acids. Note that this is something to do in controlled conditions, with proper cooling and safeguards - but my 7th grade Science Teacher, Miss Wyle, showed us and actually may be the person ultimately responsible for the course my life has taken (she was great). } } } } } } Right, sorry, I was thinking about black powder, not modern } smokeless powder which is what "gunpowder" currently means. } Andrew was typing his more detailed explanations while I was } still sending mine. } } This is a bit OT, but: } } } http://explosives.mst.edu/media/academic/explosives/documents/camp/2014ExplosivesCamp.pdf } } if you have a kid 16 or older (or if you're young enough to pass } for a high-school kid, which definitely excludes me), check this out! } } Rick Mott (guilty of making NI3 and other noisy fun stuff as } a teenager) } } ==============================Original Headers============================== } 5, 18 -- From rmott-at-pulsetor.com Thu Apr 24 10:05:42 2014 } 5, 18 -- Received: from p3plsmtpa08-05.prod.phx3.secureserver.net (p3plsmtpa08-05.prod.phx3.secureserver.net [173.201.193.106]) } 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3OF5gbY016131 } 5, 18 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 10:05:42 -0500 } 5, 18 -- Received: from [127.0.0.1] ([192.54.222.11]) } 5, 18 -- by p3plsmtpa08-05.prod.phx3.secureserver.net with } 5, 18 -- id tr5b1n0010FMozk01r5e1M; Thu, 24 Apr 2014 08:05:38 -0700 } 5, 18 -- Message-ID: {53592836.7030100-at-pulsetor.com} } 5, 18 -- Date: Thu, 24 Apr 2014 11:05:26 -0400 } 5, 18 -- From: Rick Mott {rmott-at-pulsetor.com} } 5, 18 -- User-Agent: Thunderbird 2.0.0.24 (Windows/20100228) } 5, 18 -- MIME-Version: 1.0 } 5, 18 -- To: microscopy-at-microscopy.com } 5, 18 -- Subject: Re: [Microscopy] Fw: SEM/EDS of Gunpowder } 5, 18 -- References: {201404241445.s3OEjBij000870-at-ns.microscopy.com} } 5, 18 -- In-Reply-To: {201404241445.s3OEjBij000870-at-ns.microscopy.com} } 5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 18 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
Boy has this gotten off-topic, but fun nonetheless!
I would say that the reactions (deflagration?) will still occur, so ammo should work in outer space. However, you may want to remember Newton's 3rd Law! Also the combustion products will significantly affect the local vacuum.
Cheers, Henk
On 4/24/2014 4:12 PM, mmilewski-at-comcast.net wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } So is the fear that the SEM Vacuum chamber cam blow up since the gun } powder can fuel its own combustion? So does this mean current guns and } ammo technology will still be good weapons to use in outer space? } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } werner1-at-slb.com wrote: } } } } } } } } } Modern small arms propellants (smokeless powder) are generally nitrocellulose based (there are single base and double base powders, the double base contain some nitroglycerine), granulated, and coated with deterrent coatings that encourage progressive burning - controlled deflagration - to tailor the pressure-time curve to the projectile/barrel length combination at hand. } } } } } } You can make nitrocellulose easily by nitrating cotton or paper with nitric and sulfuric acids. Note that this is something to do in controlled conditions, with proper cooling and safeguards - but my 7th grade Science Teacher, Miss Wyle, showed us and actually may be the person ultimately responsible for the course my life has taken (she was great). } } } } } } } } } } Right, sorry, I was thinking about black powder, not modern } } smokeless powder which is what "gunpowder" currently means. } } Andrew was typing his more detailed explanations while I was } } still sending mine. } } } } This is a bit OT, but: } } } } } } http://explosives.mst.edu/media/academic/explosives/documents/camp/2014ExplosivesCamp.pdf } } } } if you have a kid 16 or older (or if you're young enough to pass } } for a high-school kid, which definitely excludes me), check this out! } } } } Rick Mott (guilty of making NI3 and other noisy fun stuff as } } a teenager) } } } } ==============================Original Headers============================== } } 5, 18 -- Fromrmott-at-pulsetor.com Thu Apr 24 10:05:42 2014 } } 5, 18 -- Received: from p3plsmtpa08-05.prod.phx3.secureserver.net (p3plsmtpa08-05.prod.phx3.secureserver.net [173.201.193.106]) } } 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3OF5gbY016131 } } 5, 18 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 10:05:42 -0500 } } 5, 18 -- Received: from [127.0.0.1] ([192.54.222.11]) } } 5, 18 -- by p3plsmtpa08-05.prod.phx3.secureserver.net with } } 5, 18 -- id tr5b1n0010FMozk01r5e1M; Thu, 24 Apr 2014 08:05:38 -0700 } } 5, 18 -- Message-ID: {53592836.7030100-at-pulsetor.com} } } 5, 18 -- Date: Thu, 24 Apr 2014 11:05:26 -0400 } } 5, 18 -- From: Rick Mott {rmott-at-pulsetor.com} } } 5, 18 -- User-Agent: Thunderbird 2.0.0.24 (Windows/20100228) } } 5, 18 -- MIME-Version: 1.0 } } 5, 18 -- To:microscopy-at-microscopy.com } } 5, 18 -- Subject: Re: [Microscopy] Fw: SEM/EDS of Gunpowder } } 5, 18 -- References: {201404241445.s3OEjBij000870-at-ns.microscopy.com} } } 5, 18 -- In-Reply-To: {201404241445.s3OEjBij000870-at-ns.microscopy.com} } } 5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 5, 18 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 2, 32 -- Frommmilewski-at-comcast.net Thu Apr 24 15:10:17 2014 } 2, 32 -- Received: from qmta05.westchester.pa.mail.comcast.net (qmta05.westchester.pa.mail.comcast.net [76.96.62.48]) } 2, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3OKAG8X000636 } 2, 32 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2014 15:10:17 -0500 } 2, 32 -- Received: from omta16.westchester.pa.mail.comcast.net ([76.96.62.88]) } 2, 32 -- by qmta05.westchester.pa.mail.comcast.net with comcast } 2, 32 -- id tvxG1n0051uE5Es55wAGig; Thu, 24 Apr 2014 20:10:16 +0000 } 2, 32 -- Received: from [192.168.1.8] ([74.102.3.156]) } 2, 32 -- by omta16.westchester.pa.mail.comcast.net with comcast } 2, 32 -- id tw9w1n00D3Mxuaq3cwA1EB; Thu, 24 Apr 2014 20:10:11 +0000 } 2, 32 -- Message-ID: {53596F97.5080201-at-comcast.net} } 2, 32 -- Date: Thu, 24 Apr 2014 16:09:59 -0400 } 2, 32 -- From: Mike {mmilewski-at-comcast.net} } 2, 32 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 } 2, 32 -- MIME-Version: 1.0 } 2, 32 -- To:microscopy-at-microscopy.com } 2, 32 -- Subject: Re: [Microscopy] Re: Fw: SEM/EDS of Gunpowder } 2, 32 -- References: {201404241512.s3OFCoTF024645-at-ns.microscopy.com} } 2, 32 -- In-Reply-To: {201404241512.s3OFCoTF024645-at-ns.microscopy.com} } 2, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 2, 32 -- Content-Transfer-Encoding: 7bit } 2, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; } 2, 32 -- s=q20140121; t=1398370216; } 2, 32 -- bh=u8d3ahALTuv4qxYq+MyhJHZXcD62Bf/sK4b7DW/DXT8=; } 2, 32 -- h=Received:Received:Message-ID:Date:From:MIME-Version:To:Subject: } 2, 32 -- Content-Type; } 2, 32 -- b=NHDCDKbsSWnQFM2YyJbqbKJOQlyYuNBoMQjRiDy311WptRM2tpEPBlaX0WJqYymc4 } 2, 32 -- y1VohIpjWJTYvQ+PlIqhqsTww4uGz8/9EZ8vrDkvd/NRhHJx73kfSljGEUZnmq2far } 2, 32 -- VbA/r85FEe+tn5xgO+olzLDQgz58UkYzsBZMXq5YGaAm2vC8yzPV7dLl0drG9rjmZK } 2, 32 -- fQsJluXNCKCnCatp37KDiuG3f1kZEPYUli3ZfunCLvpJV718Ez6AuxdeTpKwqKjMiT } 2, 32 -- 4WDkbSs3h/1lvpUdvgkYeeVENDOu4IvnXK8d2l4SzU8DFfUPO15kIwAQMPVDIzQY/D } 2, 32 -- OgjkEg57Tmq/g== } ==============================End of - Headers============================== }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
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Email: matthew.hannah-at-phe.gov.uk Name: Matthew Hannah
Message: Hi, has anyone any experience of embedding Cytodex3 (or other dextran beads) in plastic for ultrathin sectioning and TEM? I have someone growing (polarized) cells on them and would like to have a look at the ultrastructure. I have tried a number of resins and protocols (although nothing longer than two days so far) but the plastic does not appear to infiltrate the dextran. Any tricks or tips gratefully received. Thanks
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Title-Subject: [Filtered] 3rd Annual OHSU-FEI Bioimaging at the Nanoscale Symposium
Message: Dear EM community, FEI Company and the Oregon Health and Sciences University (OHSU) are pleased to announce that registration is now open for the Bioimaging at the Nanoscale Symposium. The symposium will be hosted by the OHSU Center for Spatial Systems Biology and the FEI-OHSU Living Lab for Cell Biology, in Portland, Oregon, June 4th-6th, 2014. This symposium will showcase cutting edge microscopy technologies for imaging biological systems at multiple resolution scales. Presentations will cover several imaging modalities that include molecular electron microscopy (EM), super-resolution microscopy (SRM), and correlative light and electron microscopy (CLEM). World-renowned experts from these fields will discuss the most recent developments in imaging technologies and how the new tools have helped transform our understanding of biology. On June 4, FEI will host a full day of multiple workshops utilizing the suite of FEI microscopes currently installed at OHSU (Titan Krios, Tecnai with iCorr, Helios NanoLab 650). These workshops, run by FEI and OHSU personnel, will give microscopists and non-microscopists an opportunity to learn about current and cutting edge applications from FEI. Attendees are strongly encouraged to both learn from FEI and share your experiences through poster and platform presentations. Registration is free and includes complimentary breakfast, lunch and coffee for all participants. Invited speakers include: • Abraham Koster (Leiden U.) • Gregor Heiss (FEI Co.) • Robert Campbell (University of Alberta) • James Jeffers (Intel Co.) • Michael Chapman (OHSU) • Catherine Galbraith (OHSU) • James Galbraith (OHSU) • Andrew Ward (Scripps) • Gabe Lander (Scripps) • Adam Frost (U. of Utah) • Missy Wong (OHSU) • Doug Keene (Shriners Hospital for Children) • Danielle Jorgens (OHSU) • Anke Mulder (OHSU) • Gregory Scott (OHSU) • Claudia Lopez (MMC -at- OHSU) • Stefanie Kaech Petrie (ALMC -at- OHSU)
For registration click here: http://marketing.fei.com/acton/fs/blocks/showLandingPage/a/4019/p/p-012d/t/page/fm/0
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Hi everyone,
I was wondering whether it is safe to section 400 nm thick section with ultra diamond knife, or whether I have to use a histo knife
thank you
John
==============================Original Headers============================== 5, 30 -- From John.Kourtesis-at-sars.uib.no Sat Apr 26 09:29:25 2014 5, 30 -- Received: from rolf.uib.no (rolf.uib.no [129.177.6.19]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3QETOfg009465 5, 30 -- for {Microscopy-at-microscopy.com} ; Sat, 26 Apr 2014 09:29:25 -0500 5, 30 -- Received: from siv.uib.no [129.177.30.65] 5, 30 -- by rolf.uib.no for Microscopy-at-microscopy.com with esmtp (Exim 4.77) 5, 30 -- id 1We3bV-0007ub-AL; Sat, 26 Apr 2014 16:29:21 +0200 5, 30 -- Received: from SKADE.uib.no ([169.254.2.175]) by SIV.uib.no ([169.254.8.128]) 5, 30 -- with mapi id 14.02.0387.000; Sat, 26 Apr 2014 16:29:19 +0200 5, 30 -- From: John Kourtesis {John.Kourtesis-at-sars.uib.no} 5, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 30 -- Subject: 400 nm sections -what kind of knife 5, 30 -- Thread-Topic: 400 nm sections -what kind of knife 5, 30 -- Thread-Index: Ac9hW+i1AeQv2Vh9SIq/tFmIsqyB5w== 5, 30 -- Date: Sat, 26 Apr 2014 14:29:19 +0000 5, 30 -- Message-ID: {2091EFBCE3F27A4BBA00687FCC1CD934127013A2-at-SKADE.uib.no} 5, 30 -- Accept-Language: en-US, nb-NO 5, 30 -- Content-Language: en-US 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- x-originating-ip: [129.177.30.110] 5, 30 -- Content-Type: text/plain; charset="iso-8859-1" 5, 30 -- MIME-Version: 1.0 5, 30 -- X-checked-clean: by exiscan on rolf.uib.no 5, 30 -- X-Scanner: f48994048b003ef44a291575ef2089e7 http://tjinfo.uib.no/virus.html 5, 30 -- X-UiB-SpamFlag: NO UIB: -15 hits, 8.0 required 5, 30 -- X-UiB-SpamReport: spamassassin found; 5, 30 -- -15 From is listed in 'whitelist_SA' 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3QETOfg009465 ==============================End of - Headers==============================
Hi John, We routinely use old Ultra diamond knives to cut 1 um thick sections. But I must say I love my Histo diamond knife. If you are cutting a lot of thick sections a Histo diamond knife equals true happiness and I encourage you to buy one if you can afford it - 6mm will rock your world;-) best, Beth
==============================Original Headers============================== 2, 21 -- From beth-at-plantbio.uga.edu Sat Apr 26 10:57:25 2014 2, 21 -- Received: from dogwood.plantbio.uga.edu (pbmail.plantbio.uga.edu [128.192.26.3]) 2, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3QFvMNY029053 2, 21 -- for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2014 10:57:24 -0500 2, 21 -- X-Footer: cGxhbnRiaW8udWdhLmVkdQ== 2, 21 -- Received: from [96.32.176.48] ([96.32.176.48]) 2, 21 -- by dogwood.plantbio.uga.edu 2, 21 -- for microscopy-at-microscopy.com; 2, 21 -- Sat, 26 Apr 2014 11:57:19 -0400 2, 21 -- From: "Beth Richardson" {beth-at-plantbio.uga.edu} 2, 21 -- Subject: [Microscopy] 400 nm sections -what kind of knife 2, 21 -- To: microscopy {microscopy-at-microscopy.com} 2, 21 -- Message-ID: {20140426155719.fdbb0925-at-dogwood.plantbio.uga.edu} 2, 21 -- Date: Sat, 26 Apr 2014 11:57:19 -0400 2, 21 -- X-User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10_9_2) AppleWebKit/537.75.14 (KHTML, 2, 21 -- like Gecko) Version/7.0.3 Safari/537.75.14 2, 21 -- MIME-Version: 1.0 2, 21 -- Content-Type: text/plain; 2, 21 -- charset="us-ascii" 2, 21 -- Content-Transfer-Encoding: 8bit 2, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3QFvMNY029053 ==============================End of - Headers==============================
I have to agree with Beth. I used to use older ultra knives and live with some scratches, but someone brought in a 7 mm Histo knife that I made fun of ... and now I wouldn't part with it for anything. I can cut 1 micrometer sections on it and then, *without moving it or changing anything else*, silver sections.
Aloha, Tina
} I was wondering whether it is safe to section 400 nm thick section with ultra diamond knife, or whether I have to use a histo knife
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
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From drugstore-cheapest11-at-prudentialdoss.com Sun Apr 27 03:14:13 2014 Return-Path: {drugstore-cheapest11-at-prudentialdoss.com} Received: from mail1.prudentialdoss.com (host-109-168-141-72.stv.ru [109.168.141.72] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3R8EAsv018104 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 27 Apr 2014 03:14:11 -0500 Message-Id: {201404270814.s3R8EAsv018104-at-ns.microscopy.com}
Dear John, agree too with Tina & Beth, also with using "older ultra knives" prior to "resharpening". A separate {HISTO} -knife or special diamond dedicated for semithin sectioning would add some personal joy and happiness, I guess. at least one {Histo} knife (6-7 mm sectioning edge) should be standard in the prep./cutting-armamentarium when doing sectioning resin blocks for LM prior to TEM. It certainly will pay off.
Best wishes and good luck, Wolfgang
Wolfgang MUSS EM-Lab Univ. Inst. Pathology SALK-LKH (Gen. Hospital) & PMU (Paracelsus Med. Univ.) SALZBURG Austria
} -----Ursprüngliche Nachricht----- } Von: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu] } Gesendet: Samstag, 26. April 2014 22:45 } An: Muß Wolfgang } Betreff: [Microscopy] Re: 400 nm sections -what kind of knife } } ----------------------------------------------------------------------- } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } I have to agree with Beth. I used to use older ultra knives and live } with some scratches, but someone brought in a 7 mm Histo knife that I } made fun } of ... and now I wouldn't part with it for anything. I can cut 1 } micrometer sections on it and then, *without moving it or changing } anything else*, silver sections. } } Aloha, } Tina } } } I was wondering whether it is safe to section 400 nm thick section } with ultra diamond knife, or whether I have to use a histo knife } } *********************************************************************** } ***** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu } * } * Biological Electron Microscope Facility * (808) 956-6251 } * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } *********************************************************************** } ***** } } } ----------------------------------------------------------------------- } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } Re by Beth Richardson: beth-at-plantbio.uga.edu Sa 26.04.2014 18:02 } } Hi John, } We routinely use old Ultra diamond knives to cut 1µm thick sections. } But I must say I love my Histo diamond knife. If you are cutting a lot } of thick sections a Histo diamond knife equals true happiness and I } encourage you to buy one if you can afford it - 6mm will rock your } world;-) } best, } Beth } } } ==============================Original } Headers============================== } 4, 23 -- From tina-at-pbrc.hawaii.edu Sat Apr 26 15:40:40 2014 } 4, 23 -- Received: from sf-v240.pbrc.hawaii.edu (sf- } v240.pbrc.hawaii.edu [128.171.22.18]) } 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s3QKed0a017523 } 4, 23 -- for {Microscopy-at-microscopy.com} ; Sat, 26 Apr 2014 15:40:40 } -0500 } 4, 23 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) } 4, 23 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id } s3QKec9U019799 } 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=NO); } 4, 23 -- Sat, 26 Apr 2014 10:40:38 -1000 (HST) } 4, 23 -- Received: from localhost (tina-at-localhost) } 4, 23 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with } ESMTP id s3QKebaa019796; } 4, 23 -- Sat, 26 Apr 2014 10:40:38 -1000 (HST) } 4, 23 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned } process doing -bs } 4, 23 -- Date: Sat, 26 Apr 2014 10:40:37 -1000 (HST) } 4, 23 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } 4, 23 -- X-X-Sender: tina-at-sf-v240 } 4, 23 -- To: John.Kourtesis-at-sars.uib.no } 4, 23 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} } 4, 23 -- Subject: Re: [Microscopy] 400 nm sections -what kind of knife } 4, 23 -- In-Reply-To: {201404261431.s3QEVgb0010565-at-ns.microscopy.com} } 4, 23 -- Message-ID: {Pine.GSO.4.64.1404261036080.19716-at-sf-v240} } 4, 23 -- References: {201404261431.s3QEVgb0010565-at-ns.microscopy.com} } 4, 23 -- MIME-Version: 1.0 } 4, 23 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed } ==============================End of - } Headers==============================
==============================Original Headers============================== 6, 38 -- From W.Muss-at-salk.at Mon Apr 28 02:22:16 2014 6, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 6, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3S7MGBS019250 6, 38 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 02:22:16 -0500 6, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 6, 38 -- by hermes.salk.at (Postfix) with ESMTP id D36F8646B36 6, 38 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 09:22:14 +0200 (CEST) 6, 38 -- X-Scanned-By: SALK-Content-Filter 6, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 6, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 6, 38 -- with ESMTP id BXNNQXEFNaEJ for {microscopy-at-microscopy.com} ; 6, 38 -- Mon, 28 Apr 2014 09:22:14 +0200 (CEST) 6, 38 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 6, 38 -- by hermes.salk.at (Postfix) with ESMTP id ACAA6646B37 6, 38 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 09:22:14 +0200 (CEST) 6, 38 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 6, 38 -- by N2EX199.lks.local with ESMTP; 28 Apr 2014 09:26:52 +0200 6, 38 -- Received: from N1DT132.lksdom21.lks.local ([fe80::c9bf:82a7:c92e:ef25]) by 6, 38 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 6, 38 -- 14.02.0387.000; Mon, 28 Apr 2014 09:22:14 +0200 6, 38 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 6, 38 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 6, 38 -- Subject: [Microscopy] Re: 400 nm sections -what kind of knife 6, 38 -- Thread-Topic: [Microscopy] Re: 400 nm sections -what kind of knife 6, 38 -- Thread-Index: AQHPYZBivsc3/pfKokuGB5uoMJX+vpsmn0IQ 6, 38 -- Date: Mon, 28 Apr 2014 07:22:13 +0000 6, 38 -- Message-ID: {3D22E6EE65F39E448DCE416281537671207DBB8F-at-N1DT132.lksdom21.lks.local} 6, 38 -- References: {201404262044.s3QKiXmY021869-at-ns.microscopy.com} 6, 38 -- In-Reply-To: {201404262044.s3QKiXmY021869-at-ns.microscopy.com} 6, 38 -- Accept-Language: de-DE, en-US 6, 38 -- Content-Language: de-DE 6, 38 -- X-MS-Has-Attach: 6, 38 -- X-MS-TNEF-Correlator: 6, 38 -- x-originating-ip: [192.168.42.2] 6, 38 -- Content-Type: text/plain; charset="iso-8859-1" 6, 38 -- MIME-Version: 1.0 6, 38 -- Content-Transfer-Encoding: 8bit 6, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3S7MGBS019250 ==============================End of - Headers==============================
Dear friends, I’m preparing a poster (A1 size) on transmission electron microscopy (TEM) topic to decorate our characterisation centre. We have two TEMs a Jeol 2000FX and a FEI Tecnai F20 along with typical optical microscopes and SEMs, FIB plus surface analysis equipment. Our major interests are materials and physical sciences teaching and research. My manager suggested some entry level information in the poster for public visitors including 16yrs old and their parents. I used to go to conferences with research posters. It wasn’t as difficult as this. Can you give me some inspirations please? Any suggestions, text or websites etc are all appreciated. Best regards, Zhou
Dr Zhaoxia Zhou Experimental Officer Loughborough Materials Characterisation Centre (LMCC) Department of Materials Loughborough University Leicestershire LE11 3TU
Tel: 01509 223163 Fax: 01509 234225
==============================Original Headers============================== 5, 31 -- From Z.Zhou-at-lboro.ac.uk Mon Apr 28 06:01:38 2014 5, 31 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3SB1bWe013265 5, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 06:01:38 -0500 5, 31 -- Received: from [158.125.167.11] (helo=ITSCASH-4.lunet.lboro.ac.uk) 5, 31 -- by mta-1.lboro.ac.uk with esmtps (TLSv1:AES128-SHA:128) 5, 31 -- (Exim 4.80) 5, 31 -- id 1WejJL-0001q1-Dn 5, 31 -- for Microscopy-at-microscopy.com; Mon, 28 Apr 2014 12:01:23 +0100 5, 31 -- Received: from ITSMBX-4.lunet.lboro.ac.uk ([169.254.1.141]) by 5, 31 -- ITSCASH-4.lunet.lboro.ac.uk ([158.125.167.11]) with mapi id 14.03.0174.001; 5, 31 -- Mon, 28 Apr 2014 12:01:23 +0100 5, 31 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} 5, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 31 -- Subject: TEM entry level poster ideas 5, 31 -- Thread-Topic: TEM entry level poster ideas 5, 31 -- Thread-Index: Ac9iz9pWZDAqpZ19RHONLfcGXSvA/A== 5, 31 -- Date: Mon, 28 Apr 2014 11:01:22 +0000 5, 31 -- Message-ID: {FE28B2DD3D5CBB44BCF7D66B3AF8DFCC31620F51-at-ITSMBX-4.lunet.lboro.ac.uk} 5, 31 -- Accept-Language: en-GB, en-US 5, 31 -- Content-Language: en-US 5, 31 -- X-MS-Has-Attach: 5, 31 -- X-MS-TNEF-Correlator: 5, 31 -- x-originating-ip: [158.125.69.240] 5, 31 -- Content-Type: text/plain; charset="utf-8" 5, 31 -- MIME-Version: 1.0 5, 31 -- X-Scan-Signature: 924a1c3e3a3d639a1424bf4a1917f89b 5, 31 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 5, 31 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Mon, 28 Apr 2014 12:01:37 +0100 5, 31 -- Content-Transfer-Encoding: 8bit 5, 31 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s3SB1bWe013265 ==============================End of - Headers==============================
I agree with Wolfgang that your material has been poorly fixed, which complicates the interpretation of your images. The material in question appears to be coagulated Z-band material, which is extremely electron-dense fibers that sometimes appear in disrupted myocytes. I used to study muscular dystrophy in myotonic mice, and would come across myocytes with disrupted Z-band material, some of which had the coagulation patches which you are showing.
Ed Haller
Edward Haller, Lab Manager University of South Florida Department of Integrative Biology Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 (813)974-2676 ehaller-at-usf.edu Office: ISA 1046 http://biology.usf.edu/ib/research/facilities/
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Thakkar
Title-Subject: [Filtered] Ultrastructural pathology : Unknown object in muscle fiber
Message: Hi,
We found some electron dense material in the skeletal muscle fiber at subsarcolemmal as well as intermyofibrillar spaces. It can be seen in mitochondria and even in endomysium also. Higher magnification show it is not filamentous inclusion. Then what it could be.?
Clue : Skeletal muscle biopsy from 6 years male, Histopathology suggests Conginetal muscular dystrophy.
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==============================Original Headers============================== 20, 37 -- From ehaller-at-health.usf.edu Mon Apr 28 09:42:51 2014 20, 37 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 20, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3SEgopf005638 20, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 09:42:51 -0500 20, 37 -- X-ASG-Debug-ID: 1398696169-045fa83bbe240d0001-1DjkGe 20, 37 -- Received: from EXCH05.hscnet.hsc.usf.edu ([10.119.4.45]) by hscantispam.health.usf.edu with ESMTP id tmDsFlNRNy49QrVD for {Microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 10:42:49 -0400 (EDT) 20, 37 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 20, 37 -- Received: from EXCH04.hscnet.hsc.usf.edu ([169.254.2.111]) by 20, 37 -- EXCH05.hscnet.hsc.usf.edu ([169.254.6.50]) with mapi id 14.03.0181.006; Mon, 20, 37 -- 28 Apr 2014 10:42:48 -0400 20, 37 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 20, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 20, 37 -- Subject: Ultrastructural pathology : Unknown object in muscle fiber 20, 37 -- Thread-Topic: Ultrastructural pathology : Unknown object in muscle fiber 20, 37 -- X-ASG-Orig-Subj: Ultrastructural pathology : Unknown object in muscle fiber 20, 37 -- Thread-Index: AQHPYvAXAHY2m2G+zUKH2u2sH5UKcQ== 20, 37 -- Date: Mon, 28 Apr 2014 14:42:47 +0000 20, 37 -- Message-ID: {89AF5EEF4C4B8943A8D091D28859F7C898DB8306-at-EXCH04.hscnet.hsc.usf.edu} 20, 37 -- Accept-Language: en-US 20, 37 -- Content-Language: en-US 20, 37 -- X-MS-Has-Attach: 20, 37 -- X-MS-TNEF-Correlator: 20, 37 -- x-originating-ip: [10.119.4.53] 20, 37 -- Content-Type: text/plain; charset="us-ascii" 20, 37 -- MIME-Version: 1.0 20, 37 -- X-Barracuda-Connect: UNKNOWN[10.119.4.45] 20, 37 -- X-Barracuda-Start-Time: 1398696169 20, 37 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-mod/mark.cgi 20, 37 -- X-Virus-Scanned: by bsmtpd at health.usf.edu 20, 37 -- X-Barracuda-Spam-Score: 0.00 20, 37 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 20, 37 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.5317 20, 37 -- Rule breakdown below 20, 37 -- pts rule name description 20, 37 -- ---- ---------------------- -------------------------------------------------- 20, 37 -- Content-Transfer-Encoding: 8bit 20, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3SEgopf005638 ==============================End of - Headers==============================
One metric I use when communicating to laymen is "the thickness of the human hair" which is about 100 um. Everyone has a feeling for how thick a human hair is, but when you say nanometer they don't have a clue about that. So if you have a 100 nm particle, you say, "If a human hair were as thick as this room, then this object would be about 1 centimeter across!" (Well, OK, I say half an inch because the US is still in the SI backwater...)
For TEMs and SEMs, I make the analogy to an optical microscope (which a some people have experience with) or a magnifying glass, if that fails. The reason why we use electrons is because they are "smaller than light" and they let us see "different things inside."
Finally, the most important thing: pretty pictures. Be sure to have RGB pictures of something dazzling that makes them want to pore over it and see what that is. Ideally, it should have some parallels with objects people see in the big world. For example, I once used three plasmon images of a FIB lamella to make an RGB which looked like stained glass. The scientific content was pretty low, but people loved looking at the picture and it got them interested. I also have a picture of a sample prepared for AFM that looks like the Sauron's tower from Lord of the Rings -- I'll probably use that soon. I find that what you and I consider pretty pictures are not always the same as what a layman considers pretty. After all, they haven't spent the last N years looking at microscopic objects.
Hope this helps!
Zack Gainsforth Space Sciences Laboratory, UC Berkeley 7 Gauss Way Berkeley, CA 94720 cell: 626-437-9186 |--//cell: 626-437-9186--| zackg-at-ssl.berkeley.edu
On Mon, Apr 28, 2014 at 4:13 AM, |--Z.Zhou-at-lboro.ac.uk--| wrote:
--| --| --| --| --| ---------------------------------------------------------------------------- --| The Microscopy ListServer -- CoSponsor: The Microscopy Society of America --| To Subscribe/Unsubscribe -- --| http://www.microscopy.com/MicroscopyListserver --| On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html --| --| ---------------------------------------------------------------------------- --| --| Dear friends, --| I'm preparing a poster (A1 size) on transmission electron microscopy (TEM) --| topic to decorate our characterisation centre. We have two TEMs a Jeol --| 2000FX and a FEI Tecnai F20 along with typical optical microscopes and --| SEMs, FIB plus surface analysis equipment. Our major interests are --| materials and physical sciences teaching and research. My manager suggested --| some entry level information in the poster for public visitors including --| 16yrs old and their parents. I used to go to conferences with research --| posters. It wasn't as difficult as this. --| Can you give me some inspirations please? Any suggestions, text or --| websites etc are all appreciated. --| Best regards, --| Zhou --| --| Dr Zhaoxia Zhou --| Experimental Officer --| Loughborough Materials Characterisation Centre (LMCC) --| Department of Materials --| Loughborough University --| Leicestershire --| LE11 3TU --| --| Tel: 01509 223163 --| Fax: 01509 234225
I did this once years ago. We decided that one point we wanted to get across was the amazing scale of magnification provided by TEM. We found that people, especially the general public (and I also) are astounded by what high magnifications actually mean in real terms. We routinely deal with numbers like 1000x, and 150,000x without thinking. Also what does nanometre mean? We simply did some back of the envelope calculations. Then we could say things like.
We had a picture of the earth. If the earth was 1 mm diameter then the building we are in would be 1 nm long.
If we were looking at really high magnification at some sample at 500,000x (possible with the F20?) then it is equivalent to orbiting the earth and only just being able to read the text on this poster.
Our lab works on wool. So we had a micrograph of some intermediate filaments (7.6 nm diameter) and then indicated if the filament pictured on the poster was real size then the wool fibre containing it would be 640 m across.
I am sure there are other samples where a similar exercise can be done. Once people "get" what scale really means then it is easier to wow them with the technical aspects of how you manage this miracle of magnification.
Good luck Duane
AgResearch Limited | Lincoln Research Centre | New Zealand Dr Duane P Harland, Senior Scientist T +64 3 321 8710Â E duane.harland-at-agresearch.co.nz
-----Original Message----- X-from: Z.Zhou-at-lboro.ac.uk [mailto:Z.Zhou-at-lboro.ac.uk] Sent: Monday, 28 April 2014 11:16 p.m. To: Harland, Duane
Dear friends, I’m preparing a poster (A1 size) on transmission electron microscopy (TEM) topic to decorate our characterisation centre. We have two TEMs a Jeol 2000FX and a FEI Tecnai F20 along with typical optical microscopes and SEMs, FIB plus surface analysis equipment. Our major interests are materials and physical sciences teaching and research. My manager suggested some entry level information in the poster for public visitors including 16yrs old and their parents. I used to go to conferences with research posters. It wasn’t as difficult as this. Can you give me some inspirations please? Any suggestions, text or websites etc are all appreciated. Best regards, Zhou
Dr Zhaoxia Zhou Experimental Officer Loughborough Materials Characterisation Centre (LMCC) Department of Materials Loughborough University Leicestershire LE11 3TU
Tel: 01509 223163 Fax: 01509 234225
==============================Original Headers============================== 5, 31 -- From Z.Zhou-at-lboro.ac.uk Mon Apr 28 06:01:38 2014 5, 31 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3SB1bWe013265 5, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 06:01:38 -0500 5, 31 -- Received: from [158.125.167.11] (helo=ITSCASH-4.lunet.lboro.ac.uk) 5, 31 -- by mta-1.lboro.ac.uk with esmtps (TLSv1:AES128-SHA:128) 5, 31 -- (Exim 4.80) 5, 31 -- id 1WejJL-0001q1-Dn 5, 31 -- for Microscopy-at-microscopy.com; Mon, 28 Apr 2014 12:01:23 +0100 5, 31 -- Received: from ITSMBX-4.lunet.lboro.ac.uk ([169.254.1.141]) by 5, 31 -- ITSCASH-4.lunet.lboro.ac.uk ([158.125.167.11]) with mapi id 14.03.0174.001; 5, 31 -- Mon, 28 Apr 2014 12:01:23 +0100 5, 31 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} 5, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 31 -- Subject: TEM entry level poster ideas 5, 31 -- Thread-Topic: TEM entry level poster ideas 5, 31 -- Thread-Index: Ac9iz9pWZDAqpZ19RHONLfcGXSvA/A== 5, 31 -- Date: Mon, 28 Apr 2014 11:01:22 +0000 5, 31 -- Message-ID: {FE28B2DD3D5CBB44BCF7D66B3AF8DFCC31620F51-at-ITSMBX-4.lunet.lboro.ac.uk} 5, 31 -- Accept-Language: en-GB, en-US 5, 31 -- Content-Language: en-US 5, 31 -- X-MS-Has-Attach: 5, 31 -- X-MS-TNEF-Correlator: 5, 31 -- x-originating-ip: [158.125.69.240] 5, 31 -- Content-Type: text/plain; charset="utf-8" 5, 31 -- MIME-Version: 1.0 5, 31 -- X-Scan-Signature: 924a1c3e3a3d639a1424bf4a1917f89b 5, 31 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 5, 31 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Mon, 28 Apr 2014 12:01:37 +0100 5, 31 -- Content-Transfer-Encoding: 8bit 5, 31 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s3SB1bWe013265 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mikroskop-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mikroskop-at-gmail.com Name: Jack Hietpas
Title-Subject: [Filtered] ultramicrotome section preparation for SEM
Message: Hello Microscopists/ Microtomists - I am currently making ultramicrotome sections (500nm-1um, cross- and longitudinal-sections) of individual hair samples that are embedded in Embed-812. I am interested in high magnification imaging of the hair structure using SEM which should be simple enough.... however the sections are constantly folding and wrinkling once they are transferred and dried onto a sample support for SEM examination. The wrinkling usually negates proper examination. I was wondering if there are any tricks-of-the-trade that could over come this issue? Below is my current method:
1. Cut sections with ultramicrotome
2. Transfer sections (using an eyelash probe) to a gold-coated microscope coverglass (functioning as a sample support) that contains a micro-drop of water.
3. While the section is still floating on the water micro-drop, it is flattened using chloroform vapor. This works very well.
4. Either allow the water micro-drop to evaporate or gently wick the water away using a pointed piece of filter paper. IT IS HERE WHERE THE SECTIONS GET WRINKLED!
5. Gold-coat and examine using SEM.
Any suggestions on how to remove the water and have the thin section lay flat on the coverglass surface would be greatly appreciated. Perhaps a completely new substrate would work better! Is this an issue when using a TEM grid?
Thanks for your time and any information you can provide,
-Jack
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==============================Original Headers============================== 20, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 28 17:00:50 2014 20, 28 -- Received: from znl.com ([206.69.208.20]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3SM0onC005381 20, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 17:00:50 -0500 20, 28 -- Received: from localhost (localhost [127.0.0.1]) 20, 28 -- by znl.com (Postfix) with ESMTP id BDEB66F4CBB 20, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 17:00:50 -0500 (CDT) 20, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 20, 28 -- Received: from znl.com ([127.0.0.1]) 20, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 20, 28 -- with ESMTP id Maotl87DEagg for {microscopy-at-microscopy.com} ; 20, 28 -- Mon, 28 Apr 2014 17:00:41 -0500 (CDT) 20, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 20, 28 -- by znl.com (Postfix) with ESMTPA id D35026F4CA9 20, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 17:00:41 -0500 (CDT) 20, 28 -- Message-ID: {535ECF89.5000602-at-microscopy.com} 20, 28 -- Date: Mon, 28 Apr 2014 17:00:41 -0500 20, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 20, 28 -- MIME-Version: 1.0 20, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 28 -- Subject: viaWWW:ultramicrotome section preparation for SEM 20, 28 -- References: {201404281903.s3SJ3oVq022163-at-ns.microscopy.com} 20, 28 -- In-Reply-To: {201404281903.s3SJ3oVq022163-at-ns.microscopy.com} 20, 28 -- X-Forwarded-Message-Id: {201404281903.s3SJ3oVq022163-at-ns.microscopy.com} 20, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 20, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Having worked almost exclusively with tungsten SEMs, the example I have given for years is that taking 4x5 Polaroids at 100,000x would require a line of photos lined up for 100m (longer than a US football field) to show the area between two lines on a ruler that are 1mm apart. Even most Americans are at least somewhat familiar with a mm (about 1/32") and if they weren't, I'd show them my ruler.
The TEM (especially the new ones) are at least an order of magnitude better, so at a million X, the mm lines are a kilometer apart (.8 miles in the US).
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
Sent: Monday, April 28, 2014 5:23 PM To: kenconverse-at-qualityimages.biz
Hi Zhou
I did this once years ago. We decided that one point we wanted to get across was the amazing scale of magnification provided by TEM. We found that people, especially the general public (and I also) are astounded by what high magnifications actually mean in real terms. We routinely deal with numbers like 1000x, and 150,000x without thinking. Also what does nanometre mean? We simply did some back of the envelope calculations. Then we could say things like.
We had a picture of the earth. If the earth was 1 mm diameter then the building we are in would be 1 nm long.
If we were looking at really high magnification at some sample at 500,000x (possible with the F20?) then it is equivalent to orbiting the earth and only just being able to read the text on this poster.
Our lab works on wool. So we had a micrograph of some intermediate filaments (7.6 nm diameter) and then indicated if the filament pictured on the poster was real size then the wool fibre containing it would be 640 m across.
I am sure there are other samples where a similar exercise can be done. Once people "get" what scale really means then it is easier to wow them with the technical aspects of how you manage this miracle of magnification.
Good luck Duane
AgResearch Limited | Lincoln Research Centre | New Zealand Dr Duane P Harland, Senior Scientist T +64 3 321 8710Â E duane.harland-at-agresearch.co.nz
-----Original Message----- X-from: Z.Zhou-at-lboro.ac.uk [mailto:Z.Zhou-at-lboro.ac.uk] Sent: Monday, 28 April 2014 11:16 p.m. To: Harland, Duane
Dear friends, I’m preparing a poster (A1 size) on transmission electron microscopy (TEM) topic to decorate our characterisation centre. We have two TEMs a Jeol 2000FX and a FEI Tecnai F20 along with typical optical microscopes and SEMs, FIB plus surface analysis equipment. Our major interests are materials and physical sciences teaching and research. My manager suggested some entry level information in the poster for public visitors including 16yrs old and their parents. I used to go to conferences with research posters. It wasn’t as difficult as this. Can you give me some inspirations please? Any suggestions, text or websites etc are all appreciated. Best regards, Zhou
Dr Zhaoxia Zhou Experimental Officer Loughborough Materials Characterisation Centre (LMCC) Department of Materials Loughborough University Leicestershire LE11 3TU
Tel: 01509 223163 Fax: 01509 234225
==============================Original Headers============================== 5, 31 -- From Z.Zhou-at-lboro.ac.uk Mon Apr 28 06:01:38 2014 5, 31 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3SB1bWe013265 5, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 06:01:38 -0500 5, 31 -- Received: from [158.125.167.11] (helo=ITSCASH-4.lunet.lboro.ac.uk) 5, 31 -- by mta-1.lboro.ac.uk with esmtps (TLSv1:AES128-SHA:128) 5, 31 -- (Exim 4.80) 5, 31 -- id 1WejJL-0001q1-Dn 5, 31 -- for Microscopy-at-microscopy.com; Mon, 28 Apr 2014 12:01:23 +0100 5, 31 -- Received: from ITSMBX-4.lunet.lboro.ac.uk ([169.254.1.141]) by 5, 31 -- ITSCASH-4.lunet.lboro.ac.uk ([158.125.167.11]) with mapi id 14.03.0174.001; 5, 31 -- Mon, 28 Apr 2014 12:01:23 +0100 5, 31 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} 5, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 31 -- Subject: TEM entry level poster ideas 5, 31 -- Thread-Topic: TEM entry level poster ideas 5, 31 -- Thread-Index: Ac9iz9pWZDAqpZ19RHONLfcGXSvA/A== 5, 31 -- Date: Mon, 28 Apr 2014 11:01:22 +0000 5, 31 -- Message-ID: {FE28B2DD3D5CBB44BCF7D66B3AF8DFCC31620F51-at-ITSMBX-4.lunet.lboro.ac.uk} 5, 31 -- Accept-Language: en-GB, en-US 5, 31 -- Content-Language: en-US 5, 31 -- X-MS-Has-Attach: 5, 31 -- X-MS-TNEF-Correlator: 5, 31 -- x-originating-ip: [158.125.69.240] 5, 31 -- Content-Type: text/plain; charset="utf-8" 5, 31 -- MIME-Version: 1.0 5, 31 -- X-Scan-Signature: 924a1c3e3a3d639a1424bf4a1917f89b 5, 31 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 5, 31 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Mon, 28 Apr 2014 12:01:37 +0100 5, 31 -- Content-Transfer-Encoding: 8bit 5, 31 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s3SB1bWe013265 ==============================End of - Headers==============================
----- Original Message ----- X-from: {microscopylistserver-noreply-at-microscopy.com} To: {eikonika-at-otenet.gr} Sent: Tuesday, April 29, 2014 1:09 AM
Ian, thanks for your help. It had been on the point... ;-)
Here is another link a found after searching for "broad mite": http://www.google.de/imgres?imgurl=http%3A%2F%2Fwww.sel.barc.usda.gov%2Facari%2Fimages%2Fbroad%2Fegg.jpg&imgrefurl=http%3A%2F%2Fwww.sel.barc.usda.gov%2Facari%2Fcontent%2Fbroad%2Fa.html&h=331&w=419&tbnid=aqG3xtaawrHITM%3A&zoom=1&docid=lvTbzUBHnO6QkM&ei=TYBfU-uUMYeHtAaw14HwBg&tbm=isch&client=firefox-a&iact=rc&uact=3&dur=547&page=1&start=0&ndsp=67&ved=0CHwQrQMwDA
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 29.04.14 06:22, schrieb Ian Hallett: } Stefan } You are almost right the object is the egg of a mite (possibly from images we have taken a broad mite). } Ian } Ian Hallett } Plant and Food Research } Auckland New Zealand } } On Fri, Apr 18, 2014 at 6:33 AM, {stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } maybe there is someone out there before going into Easter holidays to share a bit of knowledge. } I just founds a strange detail on a specimen (the upper leaf surface of a cucumber plant): } See images here: } www.electronmicroscopy.info/customers/cucumber/index.htm {http://www.electronmicroscopy.info/customers/cucumber/index.htm} } There is also a PDF with EDS data, but it is not very specific since I put a whole lot of Platinum on the surface because of a } nanoflight movie I did with this surface: } www.electronmicroscopy.info/customers/cucumber/cucumber_eds.pdf } {http://www.electronmicroscopy.info/customers/cucumber/cucumber_eds.pdf} } A little bit of Sulfur and Ca is showing up, with Carbon. } Any idea what this might be? } } Best wishes, happy Easter, } Stefan } } -- } } } ----------------------------------------------------- } Stefan Diller - Scientific Photography } Arndtstrasse 22 } D - 97072 Wuerzburg Germany } ++49-931-7848700 {tel:%2B%2B49-931-7848700} Phone } ++49-931-7848701 {tel:%2B%2B49-931-7848701} Fax } ++49-175-7177051 {tel:%2B%2B49-175-7177051} Mobile } } Websites: } www.nanoflight.info {http://www.nanoflight.info/} } www.stefan-diller.com {http://www.stefan-diller.com/} } www.electronmicroscopy.info {http://www.electronmicroscopy.info/} } www.elektronenmikroskopie.info {http://www.elektronenmikroskopie.info/} } www.zwillingsprojekt.de {http://www.zwillingsprojekt.de/} } www.assisi.de {http://www.assisi.de/} } Anfahrt: http://Mail.map24.com/Stefan.Diller {http://mail.map24.com/Stefan.Diller} } ----------------------------------------------------- } } ==============================Original Headers============================== } 6, 20 -- From stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} Thu Apr 17 13:22:15 2014 } 6, 20 -- Received: from mailout09.t-online.de {http://mailout09.t-online.de/} (mailout09.t-online.de } {http://mailout09.t-online.de/} [194.25.134.84]) } 6, 20 -- by ns.microscopy.com {http://ns.microscopy.com/} (8.12.11.20060308/8.12.8) with ESMTP id s3HIMFj4000791 } 6, 20 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 17 Apr 2014 13:22:15 -0500 } 6, 20 -- Received: from fwd17.aul.t-online.de {http://fwd17.aul.t-online.de/} (fwd17.aul.t-online.de } {http://fwd17.aul.t-online.de/} [172.20.27.64]) } 6, 20 -- by mailout09.t-online.de {http://mailout09.t-online.de/} (Postfix) with SMTP id 1DE22448B69 } 6, 20 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 17 Apr 2014 20:21:42 +0200 (CEST) } 6, 20 -- Received: from mac-pro.speedport.ip } (ZkaTlMZBZh0uXhJpIhtna9VSLPFA7Cwazc3SY2tSipfVlb-hP2+SJ6g4jp6qRmHZcW-at-[84.174.111.253]) by fwd17.t-online.de } {http://fwd17.t-online.de/} } 6, 20 -- with esmtp id 1Waqwt-0pzLI80; Thu, 17 Apr 2014 20:22:11 +0200 } 6, 20 -- Message-ID: {53501BD3.1010400-at-t-online.de {mailto:53501BD3.1010400-at-t-online.de} } } 6, 20 -- Date: Thu, 17 Apr 2014 20:22:11 +0200 } 6, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} } } 6, 20 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 } 6, 20 -- MIME-Version: 1.0 } 6, 20 -- To: microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } 6, 20 -- Subject: Strange specimen detail - butterfly egg? } 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 6, 20 -- Content-Transfer-Encoding: 7bit } 6, 20 -- X-ID: ZkaTlMZBZh0uXhJpIhtna9VSLPFA7Cwazc3SY2tSipfVlb-hP2+SJ6g4jp6qRmHZcW } 6, 20 -- X-TOI-MSGID: 413f6eeb-72b3-4753-a0da-108da9c4dec3 } ==============================End of - Headers============================== } }
==============================Original Headers============================== 8, 22 -- From stefan.diller-at-t-online.de Tue Apr 29 05:40:06 2014 8, 22 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3TAe6C9011483 8, 22 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 05:40:06 -0500 8, 22 -- Received: from fwd21.aul.t-online.de (fwd21.aul.t-online.de [172.20.27.66]) 8, 22 -- by mailout04.t-online.de (Postfix) with SMTP id 6FB5633713A; 8, 22 -- Tue, 29 Apr 2014 12:38:31 +0200 (CEST) 8, 22 -- Received: from mac-pro.speedport.ip (XVP3HgZZohDVwYi-c3aGFym9nxVHG0VPlXDmuFWhwImcAjfrCtCF1iTIQ8JcFW8wF8-at-[84.174.102.162]) by fwd21.t-online.de 8, 22 -- with esmtp id 1Wf5SG-2qi7cG0; Tue, 29 Apr 2014 12:40:04 +0200 8, 22 -- Message-ID: {535F8184.6080308-at-t-online.de} 8, 22 -- Date: Tue, 29 Apr 2014 12:40:04 +0200 8, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 8, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 8, 22 -- MIME-Version: 1.0 8, 22 -- To: Ian Hallett {ianhallett003-at-gmail.com} , microscopy-at-microscopy.com 8, 22 -- Subject: Re: [Microscopy] Strange specimen detail - butterfly egg? 8, 22 -- References: {201404171833.s3HIXVWG016845-at-ns.microscopy.com} {CAHqF2zdqpe9hH5_2udiz4GfLxNi5DxVeNYo1DstcqN=XL9ddXQ-at-mail.gmail.com} 8, 22 -- In-Reply-To: {CAHqF2zdqpe9hH5_2udiz4GfLxNi5DxVeNYo1DstcqN=XL9ddXQ-at-mail.gmail.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- X-ID: XVP3HgZZohDVwYi-c3aGFym9nxVHG0VPlXDmuFWhwImcAjfrCtCF1iTIQ8JcFW8wF8 8, 22 -- X-TOI-MSGID: f3a6900c-3c55-4a1d-a66d-c7b88b3d08ca ==============================End of - Headers==============================
Have you tried warming the slide on a hot plate or slide warmer? One warm enough to keep the sections expanded (or to expand them with heat instead of chloroform) - what one would use to warm the sections when doing toluidine blue staining. 60 to 90 deg C.
Phil
} Email: mikroskop-at-gmail.com } Name: Jack Hietpas } } Title-Subject: [Filtered] ultramicrotome section preparation for SEM } } Message: Hello Microscopists/ Microtomists - I am currently making ultramicrotome sections } (500nm-1um, cross- and longitudinal-sections) of individual hair samples that are embedded in } Embed-812. I am interested in high magnification imaging of the hair structure using SEM which } should be simple enough.... however the sections are constantly folding and wrinkling once they are } transferred and dried onto a sample support for SEM examination. The wrinkling usually negates } proper examination. I was wondering if there are any tricks-of-the-trade that could over come this } issue? } Below is my current method: } } 1. Cut sections with ultramicrotome } } 2. Transfer sections (using an eyelash probe) to a gold-coated microscope coverglass (functioning as } a sample support) that contains a micro-drop of water. } } 3. While the section is still floating on the water micro-drop, it is flattened using chloroform } vapor. This works very well. } } 4. Either allow the water micro-drop to evaporate or gently wick the water away using a pointed } piece of filter paper. IT IS HERE WHERE THE SECTIONS GET WRINKLED! } } 5. Gold-coat and examine using SEM. } } Any suggestions on how to remove the water and have the thin section lay flat on the coverglass } surface would be greatly appreciated. Perhaps a completely new substrate would work better! Is } this an issue when using a TEM grid? } } Thanks for your time and any information you can provide, } } -Jack } } Login Host: 63.167.71.243 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } }
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 29 -- From oshel1pe-at-cmich.edu Tue Apr 29 07:20:47 2014 4, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3TCKlmT003533 4, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 07:20:47 -0500 4, 29 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 4, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s3TCKKVr013714; 4, 29 -- Tue, 29 Apr 2014 08:20:26 -0400 4, 29 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 4, 29 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 29 -- 14.3.174.1; Tue, 29 Apr 2014 08:20:23 -0400 4, 29 -- Message-ID: {535F9900.3030501-at-cmich.edu} 4, 29 -- Date: Tue, 29 Apr 2014 08:20:16 -0400 4, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 29 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 29 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 29 -- MIME-Version: 1.0 4, 29 -- To: micro {Microscopy-at-microscopy.com} , {mikroskop-at-gmail.com} 4, 29 -- Subject: Re: [Microscopy] viaWWW:ultramicrotome section preparation for SEM 4, 29 -- References: {201404282219.s3SMJdGe008267-at-ns.microscopy.com} 4, 29 -- In-Reply-To: {201404282219.s3SMJdGe008267-at-ns.microscopy.com} 4, 29 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 29 -- Content-Transfer-Encoding: 7bit 4, 29 -- X-Originating-IP: [141.209.160.122] 4, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 29 -- X-Spam-Score: 0.00 () [Hold at 6.00] _L_LLEXCH,_L_SUPPORT,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 29 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 4, 29 -- X-CanItPRO-Stream: default 4, 29 -- X-Canit-Stats-ID: 02LTMkqWq - ccbcc1acac43 - 20140429 4, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Just in case we haven't hammered the topic "can explosives and gunpowder work in a vacuum" into scrap metal, here's a link to a Wired article that covers a Mythbusters shooting in a vacuum.
It's at the end of the article, but the recoil on the bent rifle barrel was interesting too!
Stay safe........... Frank
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==============================Original Headers============================== 8, 28 -- From frank_karl-at-ardl.com Tue Apr 29 07:40:38 2014 8, 28 -- Received: from cal1-mh675b.smtproutes.com (cal1-mh675b.smtproutes.com [208.70.89.128]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3TCebOw023065 8, 28 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 07:40:38 -0500 8, 28 -- X-Katharion-ID: 1398775216.64536.cal1-mh675 8, 28 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by 8, 28 -- cal1-mh675.smtproutes.com [(192.69.16.47)] with ESMTP via TCP 8, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 29 Apr 2014 12:40:16 +0000 8, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 8, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Tue, 29 8, 28 -- Apr 2014 08:40:15 -0400 8, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 8, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 8, 28 -- {microscopy-at-microscopy.com} 8, 28 -- Date: Tue, 29 Apr 2014 08:40:14 -0400 8, 28 -- Subject: More SEM and Gunpowder 8, 28 -- Thread-Topic: More SEM and Gunpowder 8, 28 -- Thread-Index: Ac9jqCtAz/GlKCQXQ5mKwIYdIkPT3A== 8, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BF33A06A2-at-exchange2k7.ad.ardl.com} 8, 28 -- Accept-Language: en-US 8, 28 -- Content-Language: en-US 8, 28 -- X-MS-Has-Attach: 8, 28 -- X-MS-TNEF-Correlator: 8, 28 -- acceptlanguage: en-US 8, 28 -- Content-Type: text/plain; charset="us-ascii" 8, 28 -- MIME-Version: 1.0 8, 28 -- Content-Transfer-Encoding: 8bit 8, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s3TCebOw023065 ==============================End of - Headers==============================
First of all, I would not coat the coverslip until after drying the sections. Second, you might try a technique posted by another researcher on the listserver a few years ago:
"I cut1µ sections (and 5µ sections) with Diatome diamond knives mostly samples embedded in Polybed 812. I would transfer them onto a glass slide with a wooden stick or a loop. I would put the slide on a hotplate 60˚C and then invert a large glass Petri dish over the slide. I would place a cotton swab dipped in acetone under the dish with the slide. The acetone vapor + the heat would flatten the sections and as the drop of water evaporated the sections would anneal to the slide. It worked great for me."
Good luck Dean Abel Biological Sciences University of Iowa, Iowa City IA USA
I tried Dean's technique, and it works with some of my samples. What I usually do is to work with glass slides, put a 3/4 to 1 inch diameter drop of water on them with my sections on the water, and use a flame from a Bunsen burner to dry the drop of water down, passing the slide in and out of the top of the flame (I use a Touch-O-Matic burner, which gives me a small, controlled flame), and holding the slide at about a 30 degree angle so that the water dries out from under the sections. You must be careful with this technique to not get the water so hot that it boils, though!. Usually, if the technique is done right, and your slides are clean and your water is clean, the sections will tend to go to the top edge of the drop of water (uphill side of the slide), and the water will dry out from under the sections, leaving the sections perfectly flat. You need to play around with the size of your flame and how hot you get your slide, but once you get your conditions right, this technique is very consistent. After all of the water is dried off the slide, I pass the slide above the flame for a few more seconds to firmly adhere the sections to the slide before allowing the slide to cool, and before staining (if doing toluidine blue staining). In your case, once the sections are flat, you can coat them, and they are ready for the SEM. Take a diamond scribe and score your slide, break it small enough for your SEM stage, coat your sample, and you're ready for viewing.
Ed
Edward Haller, Lab Manager University of South Florida Department of Integrative Biology Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 (813)974-2676 ehaller-at-usf.edu Office: ISA 1046 http://biology.usf.edu/ib/research/facilities/
________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Monday, April 28, 2014 6:12 PM To: Haller, Edward
X-from: mikroskop-at-gmail.com ()
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mikroskop-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mikroskop-at-gmail.com Name: Jack Hietpas
Title-Subject: [Filtered] ultramicrotome section preparation for SEM
Message: Hello Microscopists/ Microtomists - I am currently making ultramicrotome sections (500nm-1um, cross- and longitudinal-sections) of individual hair samples that are embedded in Embed-812. I am interested in high magnification imaging of the hair structure using SEM which should be simple enough.... however the sections are constantly folding and wrinkling once they are transferred and dried onto a sample support for SEM examination. The wrinkling usually negates proper examination. I was wondering if there are any tricks-of-the-trade that could over come this issue? Below is my current method:
1. Cut sections with ultramicrotome
2. Transfer sections (using an eyelash probe) to a gold-coated microscope coverglass (functioning as a sample support) that contains a micro-drop of water.
3. While the section is still floating on the water micro-drop, it is flattened using chloroform vapor. This works very well.
4. Either allow the water micro-drop to evaporate or gently wick the water away using a pointed piece of filter paper. IT IS HERE WHERE THE SECTIONS GET WRINKLED!
5. Gold-coat and examine using SEM.
Any suggestions on how to remove the water and have the thin section lay flat on the coverglass surface would be greatly appreciated. Perhaps a completely new substrate would work better! Is this an issue when using a TEM grid?
Thanks for your time and any information you can provide,
-Jack
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==============================Original Headers============================== 20, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Apr 28 17:00:50 2014 20, 28 -- Received: from znl.com ([206.69.208.20]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3SM0onC005381 20, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 17:00:50 -0500 20, 28 -- Received: from localhost (localhost [127.0.0.1]) 20, 28 -- by znl.com (Postfix) with ESMTP id BDEB66F4CBB 20, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 17:00:50 -0500 (CDT) 20, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 20, 28 -- Received: from znl.com ([127.0.0.1]) 20, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 20, 28 -- with ESMTP id Maotl87DEagg for {microscopy-at-microscopy.com} ; 20, 28 -- Mon, 28 Apr 2014 17:00:41 -0500 (CDT) 20, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 20, 28 -- by znl.com (Postfix) with ESMTPA id D35026F4CA9 20, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2014 17:00:41 -0500 (CDT) 20, 28 -- Message-ID: {535ECF89.5000602-at-microscopy.com} 20, 28 -- Date: Mon, 28 Apr 2014 17:00:41 -0500 20, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 20, 28 -- MIME-Version: 1.0 20, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 28 -- Subject: viaWWW:ultramicrotome section preparation for SEM 20, 28 -- References: {201404281903.s3SJ3oVq022163-at-ns.microscopy.com} 20, 28 -- In-Reply-To: {201404281903.s3SJ3oVq022163-at-ns.microscopy.com} 20, 28 -- X-Forwarded-Message-Id: {201404281903.s3SJ3oVq022163-at-ns.microscopy.com} 20, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 20, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 33, 39 -- From ehaller-at-health.usf.edu Tue Apr 29 07:45:38 2014 33, 39 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 33, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3TCjaCc029816 33, 39 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 07:45:37 -0500 33, 39 -- X-ASG-Debug-ID: 1398775534-045fa83bbf1f7350001-1DjkGe 33, 39 -- Received: from EXCH05.hscnet.hsc.usf.edu ([10.119.4.45]) by hscantispam.health.usf.edu with ESMTP id ZuJqWe8WSkyyBZhw for {Microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 08:45:34 -0400 (EDT) 33, 39 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 33, 39 -- Received: from EXCH04.hscnet.hsc.usf.edu ([169.254.2.111]) by 33, 39 -- EXCH05.hscnet.hsc.usf.edu ([169.254.6.50]) with mapi id 14.03.0181.006; Tue, 33, 39 -- 29 Apr 2014 08:45:34 -0400 33, 39 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 33, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 33, 39 -- Subject: RE: [Microscopy] viaWWW:ultramicrotome section preparation for SEM 33, 39 -- Thread-Topic: [Microscopy] viaWWW:ultramicrotome section preparation for SEM 33, 39 -- X-ASG-Orig-Subj: RE: [Microscopy] viaWWW:ultramicrotome section preparation for SEM 33, 39 -- Thread-Index: AQHPYy7hLLDn4vPXLkagn0fwGr1zyZsoiy1z 33, 39 -- Date: Tue, 29 Apr 2014 12:45:34 +0000 33, 39 -- Message-ID: {89AF5EEF4C4B8943A8D091D28859F7C898DB9C44-at-EXCH04.hscnet.hsc.usf.edu} 33, 39 -- References: {201404282212.s3SMC0EU026114-at-ns.microscopy.com} 33, 39 -- In-Reply-To: {201404282212.s3SMC0EU026114-at-ns.microscopy.com} 33, 39 -- Accept-Language: en-US 33, 39 -- Content-Language: en-US 33, 39 -- X-MS-Has-Attach: 33, 39 -- X-MS-TNEF-Correlator: 33, 39 -- x-originating-ip: [10.119.4.53] 33, 39 -- Content-Type: text/plain; charset="utf-8" 33, 39 -- MIME-Version: 1.0 33, 39 -- X-Barracuda-Connect: UNKNOWN[10.119.4.45] 33, 39 -- X-Barracuda-Start-Time: 1398775534 33, 39 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-mod/mark.cgi 33, 39 -- X-Virus-Scanned: by bsmtpd at health.usf.edu 33, 39 -- X-Barracuda-Spam-Score: 0.00 33, 39 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 33, 39 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.5357 33, 39 -- Rule breakdown below 33, 39 -- pts rule name description 33, 39 -- ---- ---------------------- -------------------------------------------------- 33, 39 -- Content-Transfer-Encoding: 8bit 33, 39 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s3TCjaCc029816 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://ns.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ming_zhang-at-amat.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ming_zhang-at-amat.com Name: Ming Zhang
Organization: Applied Materials Company
Title-Subject: [Filtered] FIB Technician Position in Applied Materials Company
Message: Our TEM/FIB lab is located in Portland, Oregon and is equipped with a state-of-the-art FEI Osiris TEM and a Helios 600 FIB/SEM. FIB technician position will focus on TEM sample preparation of MRAM and FinFET multi-layer films by hands-on operation of ex-situ or in-situ lift-out systems as needed. Successful candidate will utilize their SEM/FIB experience to locate and successfully catch sub-micron features (as small as {100 nm) with } 90% success rate for cross-sectional TEM observations.
Term: Must be available for a minimum of 6 months. Term could be extended to 12 months. It is highly possible to turn into a regular full time position for exceptional candidates.
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Experience: 5+ years as engineering lab technician. Hands-on expert in SEM, FIB, dual beams, and TEM sample prep techniques. Hands-on experience on manual TEM sample preparation a plus.
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Organization: CAMCOR (University of Oregon)
Title-Subject: [Filtered] Now hiring: SEM-FIB expert wanted
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Richardson's Stain for Plastic Sections
Message: I am trying to stain plastic sections (LR White, Lowicryl and Embed 812 with Richardson's stain (1960's)and I have precipitate in the solution.
The precipitates are too large for filtration. Does anyone know what will solubilize them and allow for the tissues/cells to take up stain within these plastic ultrathin sections?
I look on the light microscope to see what kinds of cells I have as a preliminary region for grids on the TEM.
Thanks! Vickie
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I use Richardson's all the time. Try making up fresh components:
1% methylene blue in 1% sodium borate and 1% Azure II in distilled water
I keep the components in dark brown bottles for several years. When I need some, I mix them 1:1 (5 ml each) and suck the stain up into a 10ml syringe fitted with a 0.45 or 0.2 micrometer filter. This way, when you stain slides it's always freshly filtered. I stain for 40 sec at 60C. It seems to keep OK in the syringe for weeks to months, and the individual components in the brown bottles for years.
Aloha, Tina
} Message: I am trying to stain plastic sections (LR White, Lowicryl and Embed 812 with Richardson's } stain (1960's)and I have precipitate in the solution. } } The precipitates are too large for filtration. Does anyone know what will solubilize them and allow } for the tissues/cells to take up stain within these plastic ultrathin sections? } } I look on the light microscope to see what kinds of cells I have as a preliminary region for grids } on the TEM. } } Thanks! } Vickie } } Login Host: 141.217.26.94 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original Headers============================== } 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 29 17:49:12 2014 } 19, 28 -- Received: from znl.com ([206.69.208.20]) } 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3TMnCkX008870 } 19, 28 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 17:49:12 -0500 } 19, 28 -- Received: from localhost (localhost [127.0.0.1]) } 19, 28 -- by znl.com (Postfix) with ESMTP id BF0E86F592D } 19, 28 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 17:49:12 -0500 (CDT) } 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 19, 28 -- Received: from znl.com ([127.0.0.1]) } 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 19, 28 -- with ESMTP id 1XKFEatlGpHM for {microscopy-at-microscopy.com} ; } 19, 28 -- Tue, 29 Apr 2014 17:49:05 -0500 (CDT) } 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) } 19, 28 -- by znl.com (Postfix) with ESMTPA id 06A8C6F5922 } 19, 28 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 17:49:05 -0500 (CDT) } 19, 28 -- Message-ID: {53602C60.4070005-at-microscopy.com} } 19, 28 -- Date: Tue, 29 Apr 2014 17:49:04 -0500 } 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 } 19, 28 -- MIME-Version: 1.0 } 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 19, 28 -- Subject: viaWWW:Richardson's Stain for Plastic Sections } 19, 28 -- References: {5357CC71.4020906-at-aaem.amc.anl.gov} } 19, 28 -- In-Reply-To: {5357CC71.4020906-at-aaem.amc.anl.gov} } 19, 28 -- X-Forwarded-Message-Id: {5357CC71.4020906-at-aaem.amc.anl.gov} } 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 19, 28 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 23 -- From tina-at-pbrc.hawaii.edu Tue Apr 29 20:42:55 2014 7, 23 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3U1gspf017212 7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 20:42:55 -0500 7, 23 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 23 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id s3U1gjrm019864 7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 7, 23 -- Tue, 29 Apr 2014 15:42:45 -1000 (HST) 7, 23 -- Received: from localhost (tina-at-localhost) 7, 23 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id s3U1giB4019861; 7, 23 -- Tue, 29 Apr 2014 15:42:45 -1000 (HST) 7, 23 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 7, 23 -- Date: Tue, 29 Apr 2014 15:42:44 -1000 (HST) 7, 23 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 23 -- X-X-Sender: tina-at-sf-v240 7, 23 -- To: vakimler-at-med.wayne.edu 7, 23 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 23 -- Subject: Re: [Microscopy] viaWWW:Richardson's Stain for Plastic Sections 7, 23 -- In-Reply-To: {201404292250.s3TMoP4M009968-at-ns.microscopy.com} 7, 23 -- Message-ID: {Pine.GSO.4.64.1404291535151.11093-at-sf-v240} 7, 23 -- References: {201404292250.s3TMoP4M009968-at-ns.microscopy.com} 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Good morning all, thanks Tina, for your really appreciated reply with the practical statements,
dear Vickie,
for me (I am using a "modified Richardson's", following the recipe of Humphrey and Pittman 1974 = AMbF*-stain for semithin plastic sections) the question primarily is: why you have {precipitates - too large for filtration - in your solution} ?.
I haven't had any disturbing precipitates in my staining solutions (Toluidine blue, Richardson's, Humphrey and Pittman etc), neither in stock or in working solutions.
If you want we can discuss (also offline) possible causes of your failing and perhaps sharing practical aspects of semithin plastic section staining [which I routinely used until now from 1983 for resins Epon 812(original), Epon-substitute Glycidether 100, LX112**, EMBED 812**, also SPURR's**.. making standardized stock and working solutions since then] could/would be also interesting scientifically (:-).
[NB: * AMbF= Azure II-Methylene blue-basic Fuchsin; ** experiences on externally provided resin blocks/sections. Disclaimer: No affiliation with or financial interest/support from supplying companies/dealers]
best regards Wolfgang
Wolfgang MUSS EM-Lab, Pathology, SALK-LKH (Gen. Hosp.) & PMU SALZBURG AUSTRIA
Von: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu] Gesendet: Mittwoch, 30. April 2014 03:47 An: Muß Wolfgang Betreff: [Microscopy] Re: Richardson's Stain for Plastic Sections
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Hi, Vickie-
I use Richardson's all the time. Try making up fresh components:
1% methylene blue in 1% sodium borate and 1% Azure II in distilled water
I keep the components in dark brown bottles for several years. When I need some, I mix them 1:1 (5 ml each) and suck the stain up into a 10ml syringe fitted with a 0.45 or 0.2 micrometer filter. This way, when you stain slides it's always freshly filtered. I stain for 40 sec at 60C. It seems to keep OK in the syringe for weeks to months, and the individual components in the brown bottles for years.
Aloha, Tina
} Message: I am trying to stain plastic sections (LR White, Lowicryl and Embed 812 with Richardson's } stain (1960's)and I have precipitate in the solution. } } The precipitates are too large for filtration. Does anyone know what will solubilize them and allow } for the tissues/cells to take up stain within these plastic ultrathin sections? } } I look on the light microscope to see what kinds of cells I have as a preliminary region for grids } on the TEM. } } Thanks! } Vickie } } Login Host: 141.217.26.94 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } Microscopy-at-Microscopy.com } ============================================ } ==============================Original Headers============================== } 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 29 17:49:12 2014 } 19, 28 -- Received: from znl.com ([206.69.208.20]) } 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3TMnCkX008870 } 19, 28 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 17:49:12 -0500 } 19, 28 -- Received: from localhost (localhost [127.0.0.1]) } 19, 28 -- by znl.com (Postfix) with ESMTP id BF0E86F592D } 19, 28 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 17:49:12 -0500 (CDT) } 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 19, 28 -- Received: from znl.com ([127.0.0.1]) } 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 19, 28 -- with ESMTP id 1XKFEatlGpHM for {microscopy-at-microscopy.com} ; } 19, 28 -- Tue, 29 Apr 2014 17:49:05 -0500 (CDT) } 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) } 19, 28 -- by znl.com (Postfix) with ESMTPA id 06A8C6F5922 } 19, 28 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2014 17:49:05 -0500 (CDT) } 19, 28 -- Message-ID: {53602C60.4070005-at-microscopy.com} } 19, 28 -- Date: Tue, 29 Apr 2014 17:49:04 -0500 } 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.4.0 } 19, 28 -- MIME-Version: 1.0 } 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 19, 28 -- Subject: viaWWW:Richardson's Stain for Plastic Sections } 19, 28 -- References: {5357CC71.4020906-at-aaem.amc.anl.gov} } 19, 28 -- In-Reply-To: {5357CC71.4020906-at-aaem.amc.anl.gov} } 19, 28 -- X-Forwarded-Message-Id: {5357CC71.4020906-at-aaem.amc.anl.gov} } 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 19, 28 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
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From meds-best8-at-skyww.net Wed Apr 30 02:46:45 2014 Return-Path: {meds-best8-at-skyww.net} Received: from smtp4.skyww.net (94.41.27.57.dynamic.ufanet.ru [94.41.27.57]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s3U7khQk031490 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 30 Apr 2014 02:46:43 -0500 Message-Id: {201404300746.s3U7khQk031490-at-ns.microscopy.com}
Hi Vickie
let's take for granted that your lab has a centrifuge. I am confident that these large precipitates can be easily pelleted. I would still filter the supernatant though.
Regards, Stephane
________________________________ X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: nizets2-at-yahoo.com Sent: Wednesday, April 30, 2014 12:55 AM
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Richardson's Stain for Plastic Sections
Message: I am trying to stain plastic sections (LR White, Lowicryl and Embed 812 with Richardson's stain (1960's)and I have precipitate in the solution.
The precipitates are too large for filtration. Does anyone know what will solubilize them and allow for the tissues/cells to take up stain within these plastic ultrathin sections?
I look on the light microscope to see what kinds of cells I have as a preliminary region for grids on the TEM.
Thanks! Vickie
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Email: whiteto-at-missouri.edu Name: Tommi White
Organization: University of Missouri Electron Microscopy Core Facility
Title-Subject: [Filtered] Staff Opening
Message: The University of Missouri is currently seeking a “Research Specialist” to assist campus investigators with advanced biological specimen preparation, imaging, and analysis for both scanning and transmission electron microscopy.
The University of Missouri Electron Microscopy Core (EMC) is a campus research facility under the Office of Research. The department is equipped with 2 TEMs, 2 SEMs, and other ancillary equipment, please see http://www.emc.missouri.edu/instrumentation.html/
As a member of the University of MissouriÂ’s Electron Microscopy Core facilityÂ’s dynamic environment, the candidate will be responsible for the following:
- Optimize and implement advanced biological sample preparative techniques in accordance with investigator needs within the first year. These include the following: o high pressure freezing (Leica EMPACT) o freeze substitution (Leica AFS) o cryo-ultramicrotomy (Leica EM FCS) o automated/manual plunge-freezing (Mark IV Vitrobot) o cryo-SEM (Emitech K1250 Cryo-preparation/Cryo-transfer system) o correlative light/electron microscopy o immuno-gold labeling
- Ability to pursue multiple projects (} 5) simultaneously and accurately estimate project timelines and complete projects.
- Work directly with highly motivated individuals as part of a team and provide support when necessary
- Engage professionally with a wide variety of clients from both University of Missouri and beyond.
- Timely problem-solving with efficient experimental design/ & work-flow to get clients answers to their research questions.
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Please apply at http://hrs.missouri.edu/ for Job ID: 00010910 include coverletter, CV and 3 references.
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realname - james kammall Email - parsa319-at-yahoo.com EDUCATION - Graduate College SUBJECT_OF_QUESTION - Gilsonite ashes QUESTION - Dear Sir I have a project in Gilsonite mine.As you konw Gilsonite,Asphaltum is a natural, resinous hydrocarbon. Indeed, the mine owner is going to refine raw Gilsonite and product pure Gilsonite and related products. I need to access to knowledge and technology that enable us to remove Ash and other additional material from Gilsonite. Please guide me How to remove Ash from raw gilsonite or refine raw gilsonite.
Thanks a lot.
James Kammli
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Email: shard-at-uwm.edu Name: Steve Hardcastle
Organization: UW Milwaukee
Title-Subject: [Filtered] EPI Lamps BH2 Olympus
Message: The lamp/power supply for our epi-lamp for BH2 microscope is kaput.
What are the best alternatives for replacement? Do the LED lamp sources fit and work ($400)?
steve
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Organization: 2015 Microscopy New Zealand Conference
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2015 MICROSCOPY NEW ZEALAND CONFERENCE 2-6 February 2015, Dunedin
This biennial conference for microscopists will be held in Dunedin from Monday the 2nd to Wednesday the 4th of February 2015. It will be immediately followed by workshops scheduled for the 5th and 6th of February.
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==============================Original Headers============================== 14, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Apr 30 22:31:29 2014 14, 25 -- Received: from znl.com ([206.69.208.20]) 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s413VTEg011040 14, 25 -- for {microscopy-at-microscopy.com} ; Wed, 30 Apr 2014 22:31:29 -0500 14, 25 -- Received: from localhost (localhost [127.0.0.1]) 14, 25 -- by znl.com (Postfix) with ESMTP id 7E9726F685B 14, 25 -- for {microscopy-at-microscopy.com} ; Wed, 30 Apr 2014 22:31:29 -0500 (CDT) 14, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 25 -- Received: from znl.com ([127.0.0.1]) 14, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 25 -- with ESMTP id 1d-2lSSwP3gT for {microscopy-at-microscopy.com} ; 14, 25 -- Wed, 30 Apr 2014 22:31:24 -0500 (CDT) 14, 25 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 25 -- by znl.com (Postfix) with ESMTPA id 6CFEA6F684E 14, 25 -- for {microscopy-at-microscopy.com} ; Wed, 30 Apr 2014 22:31:24 -0500 (CDT) 14, 25 -- Message-ID: {5361C00C.7000505-at-microscopy.com} 14, 25 -- Date: Wed, 30 Apr 2014 22:31:24 -0500 14, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 25 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 25 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 14, 25 -- MIME-Version: 1.0 14, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 25 -- Subject: viaWWW: Microscopy New Zealand Conference, February 2015, Dunedin 14, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From popular-pharmacy13-at-zsttk.ru Wed Apr 30 23:32:02 2014 Return-Path: {popular-pharmacy13-at-zsttk.ru} Received: from PS.fl.zsttk.ru (host-46-241-109-138.bbcustomer.zsttk.net [46.241.109.138]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s414W0XU031962 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 30 Apr 2014 23:32:01 -0500 Message-Id: {201405010432.s414W0XU031962-at-ns.microscopy.com}
Has anyone tried the Ultra Bed low viscosity embedding resin? I stumbled onto this on the EMS site (cat #14310). Looks like it could be a good replacement for Spurr's - 2 part instead of 4 part, lower viscosity than the current version of Spurr's.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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From pharmacy-affordable9-at-ucpag.com Thu May 1 18:05:47 2014 Return-Path: {pharmacy-affordable9-at-ucpag.com} Received: from smtp3.ucpag.com (internet-188-196-40-129.narocnik.mobitel.si [188.196.40.129]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s41N5gfK023726 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 1 May 2014 18:05:44 -0500 Message-Id: {201405012305.s41N5gfK023726-at-ns.microscopy.com}
Listers,
Our met lab produces wonderful EBSD-quality specimens using standard 32 mm epoxy pots. However, I've got some specimens of porous ceramic I want to heat-treat after polishing: breaking out the specimens from epoxy would leave residual polymer in the pores, which I don't want. (Assuming I can break them from the epoxy without shattering the specimens.)
Any thoughts on how to get samples to release from epoxy pots? I'm considering embedding in superglue or wax prior to giving them to the met lab, and then using acetone to float them free from the epoxy pots later. Any thoughts on if this will work? Any better plans? The samples need to be small (10x5x0.5 mm), so I don't want to make our tech hand-polish them if I can avoid it.
Thanks Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 8, 36 -- From parishcm-at-ornl.gov Thu May 1 18:08:12 2014 8, 36 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.14]) 8, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s41N8Crr023862 8, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 1 May 2014 18:08:12 -0500 8, 36 -- X-SG: RELAYLIST 8, 36 -- X-IronPort-AV: E=Sophos;i="4.97,967,1389762000"; 8, 36 -- d="scan'208";a="79648810" 8, 36 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 8, 36 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 01 May 2014 19:08:11 -0400 8, 36 -- Received: from EXCHCS32.ornl.gov (exchcs32.ornl.gov [128.219.12.146]) 8, 36 -- (using TLSv1 with cipher RC4-SHA (128/128 bits)) 8, 36 -- (No client certificate requested) 8, 36 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id DF77410030A 8, 36 -- for {Microscopy-at-Microscopy.Com} ; Thu, 1 May 2014 19:08:11 -0400 (EDT) 8, 36 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS32.ornl.gov 8, 36 -- (128.219.12.146) with Microsoft SMTP Server (TLS) id 15.0.775.38; Thu, 1 May 8, 36 -- 2014 19:08:11 -0400 8, 36 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by 8, 36 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id 8, 36 -- 15.00.0775.031; Thu, 1 May 2014 19:07:53 -0400 8, 36 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 8, 36 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 8, 36 -- Subject: Metallography: release agent for epoxy pots? 8, 36 -- Thread-Topic: Metallography: release agent for epoxy pots? 8, 36 -- Thread-Index: Ac9lkixTInE7VmSuTDaJzo+Y29GyaA== 8, 36 -- Date: Thu, 1 May 2014 23:07:52 +0000 8, 36 -- Message-ID: {578c2630fbbb4dbd972162106fe1e977-at-EXCHCS32.ornl.gov} 8, 36 -- Accept-Language: en-US 8, 36 -- Content-Language: en-US 8, 36 -- X-MS-Has-Attach: 8, 36 -- X-MS-TNEF-Correlator: 8, 36 -- x-originating-ip: [128.219.12.132] 8, 36 -- Content-Type: text/plain; charset="us-ascii" 8, 36 -- MIME-Version: 1.0 8, 36 -- Content-Transfer-Encoding: 8bit 8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s41N8Crr023862 ==============================End of - Headers==============================
On Apr 28, 2014, at 4:14 AM, Z.Zhou-at-lboro.ac.uk wrote:
} Dear friends, } I’m preparing a poster (A1 size) on transmission electron microscopy } (TEM) topic to decorate our characterisation centre. We have two } TEMs a Jeol 2000FX and a FEI Tecnai F20 along with typical optical } microscopes and SEMs, FIB plus surface analysis equipment. Our major } interests are materials and physical sciences teaching and research. } My manager suggested some entry level information in the poster for } public visitors including 16yrs old and their parents. I used to go } to conferences with research posters. It wasn’t as difficult as this. } Can you give me some inspirations please? Any suggestions, text or } websites etc are all appreciated. } Best regards, } Zhou } Dear Zhou, You might also want to check out Project MICRO at http://www.microscopy.org/education/projectMICRO . Yours, Bill
==============================Original Headers============================== 7, 38 -- From wtivol-at-sbcglobal.net Thu May 1 18:10:57 2014 7, 38 -- Received: from nm24-vm2.access.bullet.mail.bf1.yahoo.com (nm24-vm2.access.bullet.mail.bf1.yahoo.com [216.109.115.177]) 7, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s41NAupY025851 7, 38 -- for {microscopy-at-microscopy.com} ; Thu, 1 May 2014 18:10:57 -0500 7, 38 -- Received: from [66.196.81.163] by nm24.access.bullet.mail.bf1.yahoo.com with NNFMP; 01 May 2014 23:10:56 -0000 7, 38 -- Received: from [98.139.221.156] by tm9.access.bullet.mail.bf1.yahoo.com with NNFMP; 01 May 2014 23:10:56 -0000 7, 38 -- Received: from [127.0.0.1] by smtp116.sbc.mail.bf1.yahoo.com with NNFMP; 01 May 2014 23:10:56 -0000 7, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s1024; t=1398985856; bh=Uc3YYFatdO9brt7opF6ijw4u3paaf4USSTdtlWe0QKY=; h=X-Yahoo-Newman-Id:X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:Cc:Message-Id:From:To:In-Reply-To:Content-Type:Content-Transfer-Encoding:Mime-Version:Subject:Date:References:X-Mailer; b=4316ZOSLhrem1WCyZET4gPwjkgOtb24s1ssQoHzmeC72DSG45OoYUPcqgJHsTaTPBrV9SagZRaVes4LRMx6i/isTpWxmD7UZ8yo0IPVYNqfuIrkDO6Hi8sIrpyS91f3uJrXf7WMtaKm0RLyBMt9YuDKBP5y0w659/x6hYxyzQwk= 7, 38 -- X-Yahoo-Newman-Id: 637198.44318.bm-at-smtp116.sbc.mail.bf1.yahoo.com 7, 38 -- X-Yahoo-Newman-Property: ymail-3 7, 38 -- X-YMail-OSG: YyoEIPwVM1nVjMhLerqVe9V2f5Bo_SiswJ.KW3Y5cKByM20 7, 38 -- TZbaJ3SMeKjUkwVs3K.wmFQ2EENKsdp4.WUSnoT2VFcawlPvwNUkbapx20xs 7, 38 -- sTrIXl1cMLXIBYRIuMfwYsNgS1egXeHeceijFQNhpAeLhJY9vIMgJ3L1cLa2 7, 38 -- 0.UFZtNfd07sJeeGCV2xlqCPf6ARypi8l6jbdt0zjv.Q.FYKtZKGT_pSYG91 7, 38 -- lQeOYkueeTljQcfLgKz6_pwsSim8dw4KW2ASROzvI6dpCNHTVJjtCWL8SU8B 7, 38 -- rPTVS9ToXPo6rrWeeQW.QmSdoyLcwtLYgDfv5i2xYGjQVxlyGWwGOhi0CEJm 7, 38 -- qaF7MFSjpBGmhiP7euPSWqOiZDk.7WoU9ll3sUVAy3Jw1zhJs1Ojy6YTag5Q 7, 38 -- 48ehFqNfgjTiB8J8og2B9e7ArHIVuouJjUNXnfihdfXhimBOmdV6m7AHjd3G 7, 38 -- T3LbaQJO2Xgsl03EGVoY6ELjPPLDkoGjPO8oweMCPHRL24LHlu.u6sUPjJ5u 7, 38 -- s_zb9Hy76Kjomzh3gMlb4zcJPTkc.yATG_xB1iA6.TCHAcKHn.iOzi7caHHm 7, 38 -- okMBjBwjLTo6ZnprHy7eBTsYv456.6L86Ct88rLnPjPDttrYGV05dnD.atD3 7, 38 -- _Yv51m9Ltc6LpfrqNkzgRWSh4BA-- 7, 38 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 7, 38 -- X-Rocket-Received: from [192.168.1.68] (wtivol-at-108.226.244.17 with plain [98.139.221.42]) 7, 38 -- by smtp116.sbc.mail.bf1.yahoo.com with SMTP; 01 May 2014 23:10:56 +0000 UTC 7, 38 -- Cc: microscopy-at-microscopy.com 7, 38 -- Message-Id: {50487277-5DDE-4530-9665-AD403D86EA21-at-sbcglobal.net} 7, 38 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 7, 38 -- To: Z.Zhou-at-lboro.ac.uk 7, 38 -- In-Reply-To: {201404281114.s3SBEW6R028265-at-ns.microscopy.com} 7, 38 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes 7, 38 -- Mime-Version: 1.0 (Apple Message framework v936) 7, 38 -- Subject: Re: [Microscopy] TEM entry level poster ideas 7, 38 -- Date: Thu, 1 May 2014 16:10:54 -0700 7, 38 -- References: {201404281114.s3SBEW6R028265-at-ns.microscopy.com} 7, 38 -- X-Mailer: Apple Mail (2.936) 7, 38 -- Content-Transfer-Encoding: 8bit 7, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s41NAupY025851 ==============================End of - Headers==============================
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Email: whiteto-at-missouri.edu Name: Tommi White
Organization: Central States Microscopy and Microanalysis Society
Title-Subject: [Filtered] CSMMS 2014 Spring Meeting
Message: The Central States Microscopy and Microanalysis Society is gathering on Thursday May 22nd, 2014 at MRI Global in Kansas City, Missouri for our spring meeting entitled “MacGyver Microscopy”. Hopefully everyone remembers the TV show MacGyver, and how ingenious he was repurposing common items for innovative uses. We would like to capture that essence in our meeting by highlighting new strategies, methods and techniques OR using things for purposes other than what they are meant for. Please contact Tommi White at whiteto-at-missouri.edu if you are interested in attending, giving a talk or have any questions.
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From cheapest-pharmacy1-at-singnet.com.sg Fri May 2 04:07:47 2014 Return-Path: {cheapest-pharmacy1-at-singnet.com.sg} Received: from bb220-255-103-132.singnet.com.sg (bb220-255-103-132.singnet.com.sg [220.255.103.132]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4297glm020839 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 2 May 2014 04:07:43 -0500 Message-Id: {201405020907.s4297glm020839-at-ns.microscopy.com}
X-from: margaret.bisher-at-gmail.com ()
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Email: margaret.bisher-at-gmail.com Name: Margaret Bisher
Organization: University of Pittsburgh School of Medicine
Title-Subject: [Filtered] Dendra Fluorophore
Message: Does anyone have an experience using this? I was wondering if it is possible that Dendra fluorophore might be quenched when trafficked into the acidic compartment of the cytoplasm.
Would you also have any literature reference regarding this?
Thank you, Margaret Bisher
Margaret Bisher University of Pittsburgh School of Medicine Department of Cell Biology S346 Biomedical Science Tower Pittsburgh, PA 15213
email: meb187-at-pitt.edu phone: 412-648-9565
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri May 2 18:31:03 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s42NV2lo032752 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 2 May 2014 18:31:02 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 59C4F6F7E09 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 2 May 2014 18:31:02 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id eb5Urjk6ZBeB for {microscopy-at-microscopy.com} ; 16, 28 -- Fri, 2 May 2014 18:30:56 -0500 (CDT) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id E5FD56F7DFC 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 2 May 2014 18:30:56 -0500 (CDT) 16, 28 -- Message-ID: {53642AB0.80007-at-microscopy.com} 16, 28 -- Date: Fri, 02 May 2014 18:30:56 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: Fwd: [Filtered] MicroscopyListserverviaWWW: 16, 28 -- References: {201405021832.s42IWFej028024-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201405021832.s42IWFej028024-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201405021832.s42IWFej028024-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, anybody out there who can email me a PDF copy of an x-ray examination protocol (dosimetric measurement) for the JEOL 35C SEM? Best would be, from somewhere in Germany...
Thanks, Stefan
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----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
==============================Original Headers============================== 6, 20 -- From stefan.diller-at-t-online.de Sat May 3 10:56:04 2014 6, 20 -- Received: from mailout09.t-online.de (mailout09.t-online.de [194.25.134.84]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s43Fu4vh026670 6, 20 -- for {microscopy-at-microscopy.com} ; Sat, 3 May 2014 10:56:04 -0500 6, 20 -- Received: from fwd23.aul.t-online.de (fwd23.aul.t-online.de [172.20.26.128]) 6, 20 -- by mailout09.t-online.de (Postfix) with SMTP id 5689EB2EC8 6, 20 -- for {microscopy-at-microscopy.com} ; Sat, 3 May 2014 17:55:45 +0200 (CEST) 6, 20 -- Received: from mac-pro.speedport.ip (G5sc8uZrZhvFsN0JkUtc+ioawsuz7rJULTmf4K-AzPRxAwJDU2UNH9DsuYBO3B-Q8i-at-[91.58.166.79]) by fwd23.t-online.de 6, 20 -- with esmtp id 1WgcIC-1kGrvU0; Sat, 3 May 2014 17:56:00 +0200 6, 20 -- Message-ID: {53651190.20205-at-t-online.de} 6, 20 -- Date: Sat, 03 May 2014 17:56:00 +0200 6, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de} 6, 20 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 6, 20 -- MIME-Version: 1.0 6, 20 -- To: microscopy-at-microscopy.com 6, 20 -- Subject: JEOL 35C SEM - x-ray examiniation protocol needed 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-ID: G5sc8uZrZhvFsN0JkUtc+ioawsuz7rJULTmf4K-AzPRxAwJDU2UNH9DsuYBO3B-Q8i 6, 20 -- X-TOI-MSGID: a2c538ef-718a-4f30-be35-aa2c7ebf26d5 ==============================End of - Headers==============================
From affordable-medications10-at-fulviollp.com Sat May 3 15:01:51 2014 Return-Path: {affordable-medications10-at-fulviollp.com} Received: from smtp1.fulviollp.com ([188.0.163.40]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s43K1nNd015721 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 3 May 2014 15:01:49 -0500 Message-Id: {201405032001.s43K1nNd015721-at-ns.microscopy.com}
Hi
Some times ago, as we polished ourself single cristal slices for surface science studies, we embed them in a polyster resin instead of epoxy, to be able to get them free after polishing. It was a bi-component resin for metallography (Sody 33, sold by ESCIL in France), which polymerise at room temperature and is disolved by aceton. I suppose you could get similar products in the USA by Sturers, Buhler or others. It's less hard and has more retraction than epoxy, but it works.
After polishing, one put the resin block in aceton during the night, and one find it as a jely the following morning. It's then easy to pick up the sample and after 1 or 2 washing in aceton, the samples were clean enough to be mounted on the sample holder for our study in UHV. They were not porous, but as far I remember, I think that washing them in aceton in a semi closed vessel at 40°C in an ultrasonic bath should do the job for a good cleaning.
An other way would be to try to dissolve your epoxy using the "Strip R" solvent mix sold by Epotech. But it's not a very nice product, toxic for the user and complicate to evacuate after use...
Hope it helps
Jacques
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Le 02/05/2014 01:23, parishcm-at-ornl.gov a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Listers, } } Our met lab produces wonderful EBSD-quality specimens using standard 32 mm epoxy pots. However, I've got some specimens of porous ceramic I want to heat-treat after polishing: breaking out the specimens from epoxy would leave residual polymer in the pores, which I don't want. (Assuming I can break them from the epoxy without shattering the specimens.) } } Any thoughts on how to get samples to release from epoxy pots? I'm considering embedding in superglue or wax prior to giving them to the met lab, and then using acetone to float them free from the epoxy pots later. Any thoughts on if this will work? Any better plans? The samples need to be small (10x5x0.5 mm), so I don't want to make our tech hand-polish them if I can avoid it. } } Thanks } Chad } } --------------------- } Chad M. Parish, Ph.D. } Research and Development Staff Member } Materials Science and Technology Division } Oak Ridge National Laboratory } Phone: 1 865 574 0092 } Email: parishcm-at-ornl.gov } } } } } ==============================Original Headers============================== } 8, 36 -- From parishcm-at-ornl.gov Thu May 1 18:08:12 2014 } 8, 36 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.14]) } 8, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s41N8Crr023862 } 8, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 1 May 2014 18:08:12 -0500 } 8, 36 -- X-SG: RELAYLIST } 8, 36 -- X-IronPort-AV: E=Sophos;i="4.97,967,1389762000"; } 8, 36 -- d="scan'208";a="79648810" } 8, 36 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) } 8, 36 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 01 May 2014 19:08:11 -0400 } 8, 36 -- Received: from EXCHCS32.ornl.gov (exchcs32.ornl.gov [128.219.12.146]) } 8, 36 -- (using TLSv1 with cipher RC4-SHA (128/128 bits)) } 8, 36 -- (No client certificate requested) } 8, 36 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id DF77410030A } 8, 36 -- for {Microscopy-at-Microscopy.Com} ; Thu, 1 May 2014 19:08:11 -0400 (EDT) } 8, 36 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS32.ornl.gov } 8, 36 -- (128.219.12.146) with Microsoft SMTP Server (TLS) id 15.0.775.38; Thu, 1 May } 8, 36 -- 2014 19:08:11 -0400 } 8, 36 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by } 8, 36 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id } 8, 36 -- 15.00.0775.031; Thu, 1 May 2014 19:07:53 -0400 } 8, 36 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} } 8, 36 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} } 8, 36 -- Subject: Metallography: release agent for epoxy pots? } 8, 36 -- Thread-Topic: Metallography: release agent for epoxy pots? } 8, 36 -- Thread-Index: Ac9lkixTInE7VmSuTDaJzo+Y29GyaA== } 8, 36 -- Date: Thu, 1 May 2014 23:07:52 +0000 } 8, 36 -- Message-ID: {578c2630fbbb4dbd972162106fe1e977-at-EXCHCS32.ornl.gov} } 8, 36 -- Accept-Language: en-US } 8, 36 -- Content-Language: en-US } 8, 36 -- X-MS-Has-Attach: } 8, 36 -- X-MS-TNEF-Correlator: } 8, 36 -- x-originating-ip: [128.219.12.132] } 8, 36 -- Content-Type: text/plain; charset="us-ascii" } 8, 36 -- MIME-Version: 1.0 } 8, 36 -- Content-Transfer-Encoding: 8bit } 8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s41N8Crr023862 } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 26 -- From jacques.faerber-at-ipcms.u-strasbg.fr Mon May 5 03:27:18 2014 10, 26 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s458RHm3003179 10, 26 -- for {microscopy-at-microscopy.com} ; Mon, 5 May 2014 03:27:17 -0500 10, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id C62C4242371 10, 26 -- for {microscopy-at-microscopy.com} ; Mon, 5 May 2014 10:30:44 +0200 (CEST) 10, 26 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr 10, 26 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) 10, 26 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) 10, 26 -- with ESMTP id SAWnORZwyMSm for {microscopy-at-microscopy.com} ; 10, 26 -- Mon, 5 May 2014 10:30:40 +0200 (CEST) 10, 26 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id 6A593242365 10, 26 -- for {microscopy-at-microscopy.com} ; Mon, 5 May 2014 10:30:40 +0200 (CEST) 10, 26 -- Message-ID: {53674B5F.1050309-at-ipcms.u-strasbg.fr} 10, 26 -- Date: Mon, 05 May 2014 10:27:11 +0200 10, 26 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 26 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:17.0) Gecko/20130330 Thunderbird/17.0.5 10, 26 -- MIME-Version: 1.0 10, 26 -- To: microscopy-at-microscopy.com 10, 26 -- Subject: Re: [Microscopy] Metallography: release agent for epoxy pots? 10, 26 -- References: {201405012323.s41NNdmX020186-at-ns.microscopy.com} 10, 26 -- In-Reply-To: {201405012323.s41NNdmX020186-at-ns.microscopy.com} 10, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 26 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From drugstore-best9-at-bstar.net Mon May 5 09:01:56 2014 Return-Path: {drugstore-best9-at-bstar.net} Received: from mail1.bstar.net ([77.240.168.16]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s45E1r40027707 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 5 May 2014 09:01:55 -0500 Message-Id: {201405051401.s45E1r40027707-at-ns.microscopy.com}
This is a complete and fully functional system. It has always been under service contract, since its purchase in 2005. The reason we are selling the microscope is to make space for other systems.
The confocal system has 7 laser lines (405 nm, 457 nm, 477 nm, 488 nm, 514 nm, 543 nm, and 637 nm). It is attached to a Nikon E800 upright microscope, which has a motorized XY, and a motorized Z stage. The microscope also includes four objectives 10X, 20X, 40X (oil) and 60X (oil).
Asking price: $35,000 (this is essentially the price for a Nikon E800 microscope).
Please contact me offline (307-766-3038, zzhang-at-uwyo.edu), if you are interested, or have any questions.
Thanks,
Zhaojie Zhang University of Wyoming
==============================Original Headers============================== 8, 33 -- From ZZhang-at-uwyo.edu Mon May 5 14:48:47 2014 8, 33 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2lp0207.outbound.protection.outlook.com [207.46.163.207]) 8, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s45JmlC1003321 8, 33 -- for {microscopy-at-microscopy.com} ; Mon, 5 May 2014 14:48:47 -0500 8, 33 -- Received: from DM2PR05MB463.namprd05.prod.outlook.com (10.141.105.12) by 8, 33 -- DM2PR05MB461.namprd05.prod.outlook.com (10.141.105.15) with Microsoft SMTP 8, 33 -- Server (TLS) id 15.0.939.12; Mon, 5 May 2014 19:48:29 +0000 8, 33 -- Received: from DM2PR05MB463.namprd05.prod.outlook.com ([169.254.15.157]) by 8, 33 -- DM2PR05MB463.namprd05.prod.outlook.com ([169.254.15.157]) with mapi id 8, 33 -- 15.00.0939.000; Mon, 5 May 2014 19:48:29 +0000 8, 33 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 8, 33 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 33 -- Subject: FOR SALE: BIO-Rad Radiance 2100 Laser Scanning Confocal Microscope 8, 33 -- Thread-Topic: FOR SALE: BIO-Rad Radiance 2100 Laser Scanning Confocal 8, 33 -- Microscope 8, 33 -- Thread-Index: Ac9omusAGvOh//iDTCuHc5m7S3TTpw== 8, 33 -- Date: Mon, 5 May 2014 19:48:28 +0000 8, 33 -- Message-ID: {bd73f672363d46558037837f1727ae2e-at-DM2PR05MB463.namprd05.prod.outlook.com} 8, 33 -- Accept-Language: en-US 8, 33 -- Content-Language: en-US 8, 33 -- X-MS-Has-Attach: 8, 33 -- X-MS-TNEF-Correlator: 8, 33 -- x-originating-ip: [129.72.146.142] 8, 33 -- x-forefront-prvs: 0202D21D2F 8, 33 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(428001)(164054003)(189002)(199002)(92566001)(76482001)(81542001)(81342001)(83072002)(66066001)(101416001)(31966008)(80022001)(64706001)(86362001)(46102001)(20776003)(19580405001)(79102001)(85852003)(19580395003)(75432001)(83322001)(4396001)(77982001)(2656002)(33646001)(87936001)(99286001)(99396002)(50986999)(74662001)(54356999)(74316001)(76576001)(21056001)(74502001)(24736002)(80792004);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR05MB461;H:DM2PR05MB463.namprd05.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 8, 33 -- received-spf: None (: uwyo.edu does not designate permitted sender hosts) 8, 33 -- authentication-results: spf=none (sender IP is ) 8, 33 -- smtp.mailfrom=ZZhang-at-uwyo.edu; 8, 33 -- Content-Type: text/plain; charset="us-ascii" 8, 33 -- MIME-Version: 1.0 8, 33 -- X-OriginatorOrg: uwyo.edu 8, 33 -- Content-Transfer-Encoding: 8bit 8, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s45JmlC1003321 ==============================End of - Headers==============================
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From drugstore_popular7-at-sti-travel.com Tue May 6 02:06:22 2014 Return-Path: {drugstore_popular7-at-sti-travel.com} Received: from smtp4.sti-travel.com (115.212.86.109.triolan.net [109.86.212.115] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4676KcD015303 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 6 May 2014 02:06:20 -0500 Message-Id: {201405060706.s4676KcD015303-at-ns.microscopy.com}
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Email: 12hy1-at-queensu.ca Name: Hongbing Yu
Organization: Queen's University
Title-Subject: [Filtered] TEM Diffraction pattern calibration
Message: Dear all, I am a PhD student from Queen's University. We have a new Tecnai Osiris TEM in our lab. We tried to calibrate the diffraction pattern with Standard Mo3O specimen. It was found that the long edge corresponds to the long g vector(001). According to TEM textbook edited by William, there is no rotation between diffraction pattern and image. However when we go to HRTEM, we just found that the FFT for HRTEM rotates 90 degree relative to the diffraction pattern (the sample we use is a HCP structure metal). Could anyone give me some idea to figure out the 'conflict'?
Best wishes
Hongbing
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Email: 12hy1-at-queensu.ca Name: Hongbing Yu
Organization: Queen's University
Title-Subject: [Filtered] Accutroller Model 902 required
Message: Dear all, We need a Accutroller Model 902 for gatan heating holder. If anyone who has a spare used one one for sale, please let me know and we can pay for it. Kind regards
Hongbing
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I am in need of a specimen holder for an oldie. A JEOL 100S TEM. It's a two part holder, an it's old enough that I'm hoping someone has one collecting dust on a shelf somewhere.
Thanks in advance!
--Justin A. Kraft
==============================Original Headers============================== 4, 35 -- From kraftpiano-at-gmail.com Tue May 6 08:10:13 2014 4, 35 -- Received: from mail-qc0-f181.google.com (mail-qc0-f181.google.com [209.85.216.181]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s46DADRv025717 4, 35 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2014 08:10:13 -0500 4, 35 -- Received: by mail-qc0-f181.google.com with SMTP id m20so6159650qcx.12 4, 35 -- for {microscopy-at-microscopy.com} ; Tue, 06 May 2014 06:10:12 -0700 (PDT) 4, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 35 -- d=gmail.com; s=20120113; 4, 35 -- h=subject:from:content-type:message-id:date:to 4, 35 -- :content-transfer-encoding:mime-version; 4, 35 -- bh=TvClEwOEQ59FQV6ByTVoQ4IvbZ4AWAu2HhHHZdVutMA=; 4, 35 -- b=MclC8EFdGfrKW0LEWVAXj38BTlqpl3jzOAUzoNzlGMbPftMogzscH4CGL2mjTj536N 4, 35 -- tMDldm0jq9X5X+5KOfTSekjaXdJFe21hKilfj+LT/vA4+UivV21dpFkHfi/QEOmL2lu8 4, 35 -- Vi066KuwpJG0TwN9RVPM59aEgriMDajsZE54L2hcALa7WUEf40CT7dbNc3azaEQDkvV5 4, 35 -- eMigoKGP76AEgbsgSt3UPnBmOtWj/oxo/2peag9Yly3lhb4W89chtoDft8GAquzYdLca 4, 35 -- +zlCrdNNCSwPdxOaXq97n4Z8iyeiOh+gA57NpZk6s1/oNpIVqoHO0g2cnqHRL1nPI6pd 4, 35 -- ybSw== 4, 35 -- X-Received: by 10.224.21.1 with SMTP id h1mr14009729qab.103.1399381812767; 4, 35 -- Tue, 06 May 2014 06:10:12 -0700 (PDT) 4, 35 -- Received: from [10.233.51.46] (71.sub-174-226-2.myvzw.com. [174.226.2.71]) 4, 35 -- by mx.google.com with ESMTPSA id m7sm23422680qao.20.2014.05.06.06.10.11 4, 35 -- for {microscopy-at-microscopy.com} 4, 35 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 4, 35 -- Tue, 06 May 2014 06:10:11 -0700 (PDT) 4, 35 -- Subject: In need of a holder for a JEOL 100S 4, 35 -- From: "Justin A. Kraft" {kraftpiano-at-gmail.com} 4, 35 -- Content-Type: text/plain; 4, 35 -- charset=us-ascii 4, 35 -- X-Mailer: iPhone Mail (11D201) 4, 35 -- Message-Id: {B55EFF35-247B-4953-95E4-C2CA92530675-at-gmail.com} 4, 35 -- Date: Tue, 6 May 2014 09:10:06 -0400 4, 35 -- To: microscopy-at-microscopy.com 4, 35 -- Mime-Version: 1.0 (1.0) 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s46DADRv025717 ==============================End of - Headers==============================
From drugstore_express10-at-mts.ru Tue May 6 16:05:48 2014 Return-Path: {drugstore_express10-at-mts.ru} Received: from 81.gprs.mts.ru (81.gprs.mts.ru [213.87.131.81] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s46L5h5B023924 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 6 May 2014 16:05:45 -0500 Message-Id: {201405062105.s46L5h5B023924-at-ns.microscopy.com}
On May 6, 2014, at 5:29 AM, microscopylistserver- noreply-at-microscopy.com wrote:
} Name: Hongbing Yu } } Organization: Queen's University } } Title-Subject: [Filtered] TEM Diffraction pattern calibration } } Message: Dear all, } I am a PhD student from Queen's University. We have a new Tecnai } Osiris TEM in our lab. We tried to } calibrate the diffraction pattern with Standard Mo3O specimen. It } was found that the long edge } corresponds to the long g vector(001). According to TEM textbook } edited by William, there is no } rotation between diffraction pattern and image. However when we go } to HRTEM, we just found that the } FFT for HRTEM rotates 90 degree relative to the diffraction pattern } (the sample we use is a HCP } structure metal). Could anyone give me some idea to figure out the } 'conflict'? } } Best wishes } } Hongbing } Dear Hongbing, Most scopes arrange the lens currents so that the image and diffraction modes have no rotation for as much of the magnification/ camera length range as practical. For the mags at the very low and very high ends the manual should list the rotations for both image and diffraction, from which it is easy to determine the rotation between the two modes. If that gives 90 deg, there is no 'conflict'. Yours, Bill
==============================Original Headers============================== 6, 34 -- From wtivol-at-sbcglobal.net Tue May 6 19:17:43 2014 6, 34 -- Received: from nm11-vm10.access.bullet.mail.bf1.yahoo.com (nm11-vm10.access.bullet.mail.bf1.yahoo.com [216.109.114.233]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s470Hg0f026485 6, 34 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2014 19:17:42 -0500 6, 34 -- Received: from [66.196.81.158] by nm11.access.bullet.mail.bf1.yahoo.com with NNFMP; 07 May 2014 00:17:42 -0000 6, 34 -- Received: from [98.138.104.97] by tm4.access.bullet.mail.bf1.yahoo.com with NNFMP; 07 May 2014 00:17:42 -0000 6, 34 -- Received: from [127.0.0.1] by smtp117.sbc.mail.ne1.yahoo.com with NNFMP; 07 May 2014 00:17:42 -0000 6, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s1024; t=1399421862; bh=8jvQJA1XorFOeapVuF0Cm6ActvUGtdQKOiBHkNdZ1F4=; h=X-Yahoo-Newman-Id:X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:Message-Id:From:To:In-Reply-To:Content-Type:Content-Transfer-Encoding:Mime-Version:Subject:Date:References:X-Mailer; b=KqKAw4MaA8BrdN79z8mWbPx0ygyM2f+oJKuZbet6rrSKcJ7HAoC/DMPqnOzAJOt8DBjyg8h9927jFlzxs3VyVsmkSMK/kAMuvEirQ/uP0z8UFSke2dWvnotjciLSJPXfsPnoQtCF9/AdWAD2zs6P9iDMShB8O/JsJkanr0s9Pks= 6, 34 -- X-Yahoo-Newman-Id: 333967.59442.bm-at-smtp117.sbc.mail.ne1.yahoo.com 6, 34 -- X-Yahoo-Newman-Property: ymail-3 6, 34 -- X-YMail-OSG: L01FwfoVM1kBW57mhHEnDnGuC5HGzgUqWXYRDGzUkoLcSg_ 6, 34 -- vJZ0zA2ciugl4xeWzlKvx8fLlQflRd2p_v3uQRZC0EOGtCiKgPbvN_wUOwcV 6, 34 -- BuCm6Vuih9bRiQeBSbz8JTJnkNs60q..tWzNyP_YOOlBNs_xSM6yb1FyMN3K 6, 34 -- DIs9tUSCPCs1BxoU55PDZf3u.hcq4h.rKUhPtZhCqBmVBMs.ELyL0dEECNUL 6, 34 -- ppUjYEkGcYxBHO2fHnkN1.mPwqnip7IORMCKKiSenjkB1n4yMPd4tCcE9xhS 6, 34 -- 9GHRbo2vRUwuETAivVC3HTUFIePs11kwAhqKu0LObfRWWSHyFqg0ZRciBkpO 6, 34 -- Ru4VRNJU1i5zWKMG_O6aDowusBheS_.iEBJxddFprd8wyqpx18oKeYCFUfqU 6, 34 -- UOEfEvG7y2xBK_JkluqqNTGj91.qo.7Aq6EOnLvrgSVd2El.WL5pyEInGIe5 6, 34 -- RmU7w9.ee8L8Yrw_6a2aiWOrR.FJcWi02kPJX4RU3mQhvh5iJ1Rpc1HowFK0 6, 34 -- bJlD754yA9tCt0NXU91gWoH4g.UuzjCgVJ3k- 6, 34 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 34 -- X-Rocket-Received: from [192.168.1.68] (wtivol-at-108.226.244.17 with plain [98.138.31.74]) 6, 34 -- by smtp117.sbc.mail.ne1.yahoo.com with SMTP; 07 May 2014 00:17:42 +0000 UTC 6, 34 -- Message-Id: {91B73BA4-6080-49B4-9D7C-BA28E7B36C33-at-sbcglobal.net} 6, 34 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 34 -- To: microscopy-at-microscopy.com, 12hy1-at-queensu.ca 6, 34 -- In-Reply-To: {201405061229.s46CTQEd016723-at-ns.microscopy.com} 6, 34 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 34 -- Content-Transfer-Encoding: 7bit 6, 34 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 34 -- Subject: Re: [Microscopy] viaWWW:TEM Diffraction pattern calibration 6, 34 -- Date: Tue, 6 May 2014 17:17:40 -0700 6, 34 -- References: {201405061229.s46CTQEd016723-at-ns.microscopy.com} 6, 34 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
This is probably a topic discussed at this list-server before: TEM magnetic materials. The University of Delaware has substantial research in this area and multiple groups need TEM support for magnetic material characterizations. We do take some precautions but still occasionally run into issues. Instead of specifying more details, I would like to know what options or policies your labs have or implement in dealing with magnetic particles, thin films, or foils from bulk magnets?
Many thanks in advance!
Best regards,
******************************************************* Chaoying Ni, PhD Associate Professor, Materials Science and Engineering Director, W. M. Keck Center for Advanced Microscopy and Microanalysis http://eml.masc.udel.edu; http://www.mseg.udel.edu
Facility location: Interdisciplinary Science and Engineering Laboratory 221 Academic Street, Newark, DE 19716 http://www.udel.edu/iselab (302) 831-6359 (Phone); (302) 831-4545 (Fax)
Mailing address: University of Delaware 201 duPont Hall, Newark, DE 19716 *******************************************************
==============================Original Headers============================== 9, 27 -- From cni-at-udel.edu Tue May 6 20:03:52 2014 9, 27 -- Received: from EDGE1.win.udel.edu (edge1.win.udel.edu [128.175.68.31]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4713qos013539 9, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 6 May 2014 20:03:52 -0500 9, 27 -- Received: from HUB2.win.udel.edu (128.175.175.15) by EDGE1.win.udel.edu 9, 27 -- (128.175.68.31) with Microsoft SMTP Server (TLS) id 14.3.174.1; Tue, 6 May 9, 27 -- 2014 21:03:13 -0400 9, 27 -- Received: from MAILBOX2.win.udel.edu ([169.254.3.242]) by HUB2.win.udel.edu 9, 27 -- ([10.220.2.31]) with mapi id 14.03.0174.001; Tue, 6 May 2014 21:03:51 -0400 9, 27 -- From: "Ni, Chaoying" {cni-at-udel.edu} 9, 27 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 27 -- Subject: TEM magnetic materials - multiuser facility operation rules or 9, 27 -- policy options 9, 27 -- Thread-Topic: TEM magnetic materials - multiuser facility operation rules or 9, 27 -- policy options 9, 27 -- Thread-Index: Ac9pj/QlSS78BWuMTfGWWsR0K8VuUA== 9, 27 -- Date: Wed, 7 May 2014 01:03:51 +0000 9, 27 -- Message-ID: {2F0F43748D54D945B9DD8C86C56FF38C8543E3D7-at-MAILBOX2.win.udel.edu} 9, 27 -- Accept-Language: en-US 9, 27 -- Content-Language: en-US 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- x-originating-ip: [10.210.1.1] 9, 27 -- Content-Type: text/plain; charset="iso-8859-1" 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4713qos013539 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both boris.reznik-at-kit.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: boris.reznik-at-kit.edu Name: Boris
Organization: KIT
Title-Subject: [Filtered] SAED-Software
Message: Dear all,
we are looking for a suitable software allowing to calculate SAED spot patterns. The aim of our investigation is to determine the zone axis of minerals. Using an experimental SAED pattern three diffraction vectors and angles between them are measured. These parameters should be compared with the calculated one by taking into account the lattice constants, space group and extinction conditions of the studied crystal.
Do you know a software allowing to solve this task?
With regards Boris
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed May 7 08:57:58 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s47DvwN1012111 17, 28 -- for {microscopy-at-microscopy.com} ; Wed, 7 May 2014 08:57:58 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id 5E76F6FB32E 17, 28 -- for {microscopy-at-microscopy.com} ; Wed, 7 May 2014 08:57:58 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id 9GVSSBwzEcAx for {microscopy-at-microscopy.com} ; 17, 28 -- Wed, 7 May 2014 08:57:53 -0500 (CDT) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 4CD1E6FB321 17, 28 -- for {microscopy-at-microscopy.com} ; Wed, 7 May 2014 08:57:53 -0500 (CDT) 17, 28 -- Message-ID: {536A3BE1.1010803-at-microscopy.com} 17, 28 -- Date: Wed, 07 May 2014 08:57:53 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW: SAED-Software 17, 28 -- References: {201405071315.s47DFmaD010667-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201405071315.s47DFmaD010667-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201405071315.s47DFmaD010667-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We recently acquired a Fischinone dual-axis holder (model 2040) for room-temperature tomography. To our surprise, this holder can only be tilted (+/- 60 degree) for the very center of grid without touching pole-pieces. We have used a 2020 holder so far and are extremely happy with it. We use it on a FEI Tecnai F20 with STEM configuration (X-twin lenses).
Does anyone encounter this issue before? If yes, how did you get around it?
Thank you in advance for your help.
Xinran Liu
________________________________________
Xinran Nick Liu, M.D. & Ph.D. Director of Electron Microscopy Core Facility Research Scientist in Cell Biology Yale University School of Medicine 333 Cedar Street, SHM I-E16A New Haven, CT 06520 Office: 203.785.4050 Lab: 203.785.5390 http://medicine.yale.edu/ccmi/em
==============================Original Headers============================== 11, 35 -- From xinran.liu-at-yale.edu Wed May 7 09:27:19 2014 11, 35 -- Received: from vm-emlprdomg-06.its.yale.edu (vm-emlprdomg-06.its.yale.edu [130.132.50.169]) 11, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s47ERJWJ031555 11, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 7 May 2014 09:27:19 -0500 11, 35 -- Received: from x10-caht3.yu.yale.edu (x10-caht3.its.yale.edu [130.132.61.67]) 11, 35 -- by vm-emlprdomg-06.its.yale.edu (8.14.5/8.14.5) with ESMTP id s47ERIxB007010 11, 35 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 11, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 7 May 2014 10:27:18 -0400 11, 35 -- Received: from X10-MBX13.yu.yale.edu ([169.254.13.146]) by 11, 35 -- x10-caht3.yu.yale.edu ([130.132.61.67]) with mapi id 14.03.0174.001; Wed, 7 11, 35 -- May 2014 10:27:18 -0400 11, 35 -- From: "Liu, Xinran" {xinran.liu-at-yale.edu} 11, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 35 -- Subject: Fischione tomography holder 11, 35 -- Thread-Topic: Fischione tomography holder 11, 35 -- Thread-Index: AQHPagByF0z5P35oXEaUQEO2eT3qzA== 11, 35 -- Date: Wed, 7 May 2014 14:27:17 +0000 11, 35 -- Message-ID: {CF8FBB03.1D8F9%xinran.liu-at-yale.edu} 11, 35 -- Accept-Language: en-US 11, 35 -- Content-Language: en-US 11, 35 -- X-MS-Has-Attach: 11, 35 -- X-MS-TNEF-Correlator: 11, 35 -- user-agent: Microsoft-MacOutlook/14.3.9.131030 11, 35 -- x-originating-ip: [130.132.61.93] 11, 35 -- Content-Type: text/plain; charset="us-ascii" 11, 35 -- Content-ID: {7AF83682A98BEF42B3DF5CE5668B67C0-at-connect.yale.edu} 11, 35 -- MIME-Version: 1.0 11, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.11.96,1.0.14,0.0.0000 11, 35 -- definitions=2014-05-07_03:2014-05-07,2014-05-07,1970-01-01 signatures=0 11, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 11, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 11, 35 -- engine=7.0.1-1402240000 definitions=main-1405070170 11, 35 -- X-Yale-Not-Spam: For more info see: http://www.yale.edu/email/spam/content.html 11, 35 -- Content-Transfer-Encoding: 8bit 11, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s47ERJWJ031555 ==============================End of - Headers==============================
 I use Single Crystal. It works very well for many cases. It is not really the tool for indexing a completely unknown crystal. It has some zone searching features, but that can still require elbow grease.
Pros: If you have a pretty good idea what the crystal is, or you know it is one of a few (I usually do EDS first so I do have a decent idea what I'm looking at) then this is hard to beat because you can rotate the crystal live. (Also great for getting a feel of the reciprocal space of a given crystal BEFORE your TEM session so you go quickly to a good zone.) You can easily see relative goniometer positions. If you know what a specific reflection is, you can tilt the crystal orthogonal to it to find a specific zone axis, so it has been useful in recognizing that sometimes there are similar looking zones and with other software you may get fooled. For ALCHEMI, you can see when you are aligned along a certain column of atoms easily. You can adjust the unit cell parameters easily for comparison between a couple phases.
Cons: Kinematic only. Searching for complete unknown zones is a bit tricky. I recommend you get on a good zone axis when taking your patterns, don't just try to index a [15,3,7] zone (although, I have managed it in the past).
http://www.crystalmaker.com/singlecrystal/
Zack
(Disclosure: no financial interest but I have done some beta testing.)
The X-twin lens was designed not as a 'STEM' configuration but to maximise EDX performance (without compromising STEM probe size). This involved modifications to the pole pieces to allow an EDX detector to be moved closer to the specimen and hence improve the solid angle, as well as an extra shield on the lower pole piece to reduce backscatter and stray X-rays (http://dx.doi.org/10.1017/S1431927605505348).
Unfortunately this does mean that these modifications reduce the tilt range of most holders when compared to a SuperTWIN lens (more standard for a 'STEM' configuration). The 2040 holder is quite a bit thicker than the 2020, and will more than likely lead to pole-touch in a narrower pole piece gap. For your information the 2040 has no tilt restrictions in a SuperTWIN lens, but maximum useful tilt angle is ~+/- 70 degrees due to holder shadowing.
As this a geometrical problem I don't see an easy solution. Use the 2020 and take the specimen out and rotate it 90 degrees between dual-axis tilting would be my suggestion! The loss of information between 60-70 degrees will severely affect reconstruction (much more so than 70-80). We mostly use our 2040 to orient specimen features perpendicular to the tilt axis prior to tomography (or as a really high tilt DT holder for crystallography).
Matthew
On 8/05/2014 12:36 AM, xinran.liu-at-yale.edu wrote: } Good morning listers, } } We recently acquired a Fischinone dual-axis holder (model 2040) for } room-temperature tomography. To our surprise, this holder can only be } tilted (+/- 60 degree) for the very center of grid without touching } pole-pieces. We have used a 2020 holder so far and are extremely happy } with it. We use it on a FEI Tecnai F20 with STEM configuration (X-twin } lenses). } } Does anyone encounter this issue before? If yes, how did you get around it? } } Thank you in advance for your help. } } Xinran Liu } } ________________________________________ } } Xinran Nick Liu, M.D. & Ph.D. } Director of Electron Microscopy Core Facility } Research Scientist in Cell Biology } Yale University School of Medicine } 333 Cedar Street, SHM I-E16A } New Haven, CT 06520 } Office: 203.785.4050 } Lab: 203.785.5390 } http://medicine.yale.edu/ccmi/em } -- Dr M.Weyland, Associate Professor & Titan Manager -------------------------------------------------------------------------- Monash Centre for Electron Microscopy Bldg 81 Monash University Victoria 3800 Australia. -------------------------------------------------------------------------- www.mcem.monash.edu.au ------------------------------------------------------------------------- Ph: (+61) 3 990 59026 --- Fax: (+61) 3 990 53600 --------------------------------------------------------------------------
==============================Original Headers============================== 7, 41 -- From matthew.weyland-at-monash.edu Wed May 7 18:17:36 2014 7, 41 -- Received: from na3sys009aog135.obsmtp.com (na3sys009aog135.obsmtp.com [74.125.149.84]) 7, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s47NHZpn013544 7, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 7 May 2014 18:17:36 -0500 7, 41 -- Received: from mail-pa0-f51.google.com ([209.85.220.51]) (using TLSv1) by na3sys009aob135.postini.com ([74.125.148.12]) with SMTP 7, 41 -- ID DSNKU2q/D9w6MgY3VKqfAdyeX4sqCYG6b/Kk-at-postini.com; Wed, 07 May 2014 16:17:36 PDT 7, 41 -- Received: by mail-pa0-f51.google.com with SMTP id kq14so1788411pab.24 7, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 07 May 2014 16:17:34 -0700 (PDT) 7, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 41 -- d=1e100.net; s=20130820; 7, 41 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 7, 41 -- :subject:references:in-reply-to:content-type 7, 41 -- :content-transfer-encoding; 7, 41 -- bh=isM22Eli8lUNyOmGEBSktxoIojFpzEdVWX9Dn/rxA6A=; 7, 41 -- b=efEDA6NbCeegdpVdAKPttcJ7cqSC+vsT2/rtQd/6j2r7Lb4Daas+MTwJQfdWTEEZ1P 7, 41 -- UIWsBg3+XPAd8QWQfG8v6gvrGWBTKRukwdaVro2qD6gKVVGxzXwbgwBmK/sXIYO8/xng 7, 41 -- 55osokdjR9DrmYepj6d5ThSJTDRLOWhKHAb3tVwzC5MnZ43xz24sZhD9GpbUXQCZsFIR 7, 41 -- ezJFMmJQjYrvcKawLeVbuDQVMQD3ouRZAq7gAgIsppVpQpudVDi9PrOwhONvxR1N4xqt 7, 41 -- vBckkTDH2ytWqqWJ9/I08d2LAAOoBXWMduw7c4R/HZN3tnPzX87NMoTxRTYHzvDDyvSH 7, 41 -- xAPg== 7, 41 -- X-Received: by 10.66.171.76 with SMTP id as12mr192374pac.52.1399504271874; 7, 41 -- Wed, 07 May 2014 16:11:11 -0700 (PDT) 7, 41 -- X-Gm-Message-State: ALoCoQnxl4ohWq8rpgYx3mzRBkJpcQN+5A5NtXl8jZDDaZluFpD/qkoNor2s7k0D/twcpqjyfMWPsw2VzkfjATgkeyOFVIt1eOd/RDbGpZGZWk1y2v0nG76Vm0gP6kZSfmdH4ApZX3xI 7, 41 -- X-Received: by 10.66.171.76 with SMTP id as12mr192354pac.52.1399504271760; 7, 41 -- Wed, 07 May 2014 16:11:11 -0700 (PDT) 7, 41 -- Received: from [49.127.76.118] (dyn-49-127-76-118.its.monash.edu.au. [49.127.76.118]) 7, 41 -- by mx.google.com with ESMTPSA id og3sm5131849pbc.48.2014.05.07.16.11.10 7, 41 -- for {Microscopy-at-microscopy.com} 7, 41 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 7, 41 -- Wed, 07 May 2014 16:11:10 -0700 (PDT) 7, 41 -- Message-ID: {536ABD81.1000409-at-monash.edu} 7, 41 -- Date: Thu, 08 May 2014 09:10:57 +1000 7, 41 -- From: Matthew Weyland {matthew.weyland-at-monash.edu} 7, 41 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 7, 41 -- MIME-Version: 1.0 7, 41 -- To: Microscopy-at-microscopy.com 7, 41 -- Subject: Re: Fischione tomography holder 7, 41 -- References: {201405071436.s47Ea3bZ016329-at-ns.microscopy.com} 7, 41 -- In-Reply-To: {201405071436.s47Ea3bZ016329-at-ns.microscopy.com} 7, 41 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: bsmit112-at-jhmi.edu Name: Barbara Smith
Organization: Johns Hopkins University
Title-Subject: [Filtered] Sorvall JB4 Microtome
Message: We recently acquired a Sorvall JB4 microtome that is supposed to do both paraffin and plastic sectioning. Unfortunately, it came without the paraffin chuck and the blade holder. Does anyone know of a group or have such an accessory that they would be willing to part with? Or have any suggestions on how to modify? Thank you.
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I am required to eliminate all Windows XP systems and replace them with Windows 7 systems. I have been unable to determine how this may affect a JEOL1400 or if new software and drivers are required. Has anyone completed this OS upgrade on a JEOL1400? Thanks, Larry
-- Larry Ackerman, Specialist Electron Microscopy Lab Manager, Diabetes & Endocrinology Research Center Microscopy Core UCSF, Dept. of Anatomy, Rm S657 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-999-4758
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We got around this by getting the IT guys to set up a separate VLAN for our old XP systems. We have at least 3 systems that cannot be upgraded. This seems to work fine - data can be uploaded to the stand-alone server and people can then download it to their computers. This could be an interim solution even if you do eventually convert all to 7.
cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334M 61 2 468 966 713 E rosemary.white-at-csiro.au
On 8/05/14 11:16 AM, "larry.ackerman-at-ucsf.edu" {larry.ackerman-at-ucsf.edu} wrote:
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From drugstore_reasonable14-at-js-llp.com Thu May 8 00:39:30 2014 Return-Path: {drugstore_reasonable14-at-js-llp.com} Received: from mail3.js-llp.com ([186.34.51.47]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s485dQPG029469 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 8 May 2014 00:39:27 -0500 Message-Id: {201405080539.s485dQPG029469-at-ns.microscopy.com}
X-from: roger.ristau-at-uconn.edu ()
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: Univ of Connecticut
Title-Subject: [Filtered] cleaning evaporator
Message: Does anyone have an effective method for cleaning metal deposition from the inside of an evaporator bell-jar?
Cheers Roger Ristau University of Connecticut
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu May 8 09:00:58 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48E0wpr003979 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2014 09:00:58 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 230746FBDFC 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2014 09:00:58 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id QLRTgW7xknwM for {microscopy-at-microscopy.com} ; 13, 28 -- Thu, 8 May 2014 09:00:51 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 3A36E6FBDEA 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2014 09:00:51 -0500 (CDT) 13, 28 -- Message-ID: {536B8E12.4000909-at-microscopy.com} 13, 28 -- Date: Thu, 08 May 2014 09:00:50 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW: cleaning evaporator 13, 28 -- References: {201405081250.s48Covld002882-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201405081250.s48Covld002882-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201405081250.s48Covld002882-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Organization: Centers for Disease Control and Prevention
Title-Subject: [Filtered] Osmium Tetroxide SOP
Message: Our Safety Office would like for us to write up an SOP in case of a spill of osmium tetroxide. I thought that there is no need to reinvent the wheel, and would like to ask for other laboratories who already have an SOP written up if they would share it with me, offline at CGoldsmith-at-cdc.gov.
Thank you,
Cynthia Goldsmith, MGS Infectious Diseases Pathology Branch Centers for Disease Control and Prevention Atlanta, GA 30333 (404)639-3306
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu May 8 09:01:45 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48E1jPl004656 19, 28 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2014 09:01:45 -0500 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 729EA6FBE69 19, 28 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2014 09:01:45 -0500 (CDT) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id MKiS6lvPbMrQ for {microscopy-at-microscopy.com} ; 19, 28 -- Thu, 8 May 2014 09:01:40 -0500 (CDT) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id CA9346FBE57 19, 28 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2014 09:01:40 -0500 (CDT) 19, 28 -- Message-ID: {536B8E44.2080707-at-microscopy.com} 19, 28 -- Date: Thu, 08 May 2014 09:01:40 -0500 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:Osmium Tetroxide SOP 19, 28 -- References: {201405081352.s48DqGiO003880-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201405081352.s48DqGiO003880-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201405081352.s48DqGiO003880-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I use Bon Ami, a non-abrasive kitchen cleaner, a damp paper towel, and scrub. Nothing else.
Phil
} Email: roger.ristau-at-uconn.edu } Name: Roger Ristau } } Organization: Univ of Connecticut } } Title-Subject: [Filtered] cleaning evaporator } } Message: Does anyone have an effective method for cleaning metal deposition from the inside of an } evaporator bell-jar? } } Cheers } Roger Ristau } University of Connecticut -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 29 -- From oshel1pe-at-cmich.edu Thu May 8 09:29:30 2014 3, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48ETU31010337 3, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2014 09:29:30 -0500 3, 29 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 3, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s48ESnn2004473; 3, 29 -- Thu, 8 May 2014 10:29:27 -0400 3, 29 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 3, 29 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 3, 29 -- 14.3.174.1; Thu, 8 May 2014 10:29:09 -0400 3, 29 -- Message-ID: {536B94B7.3040107-at-cmich.edu} 3, 29 -- Date: Thu, 8 May 2014 10:29:11 -0400 3, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 29 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 29 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 29 -- MIME-Version: 1.0 3, 29 -- To: {roger.ristau-at-uconn.edu} , micro {Microscopy-at-microscopy.com} 3, 29 -- Subject: Re: [Microscopy] viaWWW: cleaning evaporator 3, 29 -- References: {201405081419.s48EJd4p006762-at-ns.microscopy.com} 3, 29 -- In-Reply-To: {201405081419.s48EJd4p006762-at-ns.microscopy.com} 3, 29 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- X-Originating-IP: [141.209.160.122] 3, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 29 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 29 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 3, 29 -- X-CanItPRO-Stream: default 3, 29 -- X-Canit-Stats-ID: 02LXqtrRR - 5eaf611ced12 - 20140508 3, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
We have a Phillips (fei) XL40 ESEM (circa 2000) with the latest microscope control software. The installed EDAX Phoenix EDS system is failing. The x-ray detector (EDAX detecting unit PV7761/53ME) seems to be functioning properly. An EDAX Genesis system has been located and we would appreciate any information members of the group have regarding:
1) Compatibility of the installed x-ray detector with EDAX Genesis.
2) Configuring the serial interface between EDAX Genesis and fei's microscope control.
3) Any gotchas...
Thank you,
Owen Mills Michigan Tech
==============================Original Headers============================== 7, 34 -- From opmills-at-mtu.edu Thu May 8 10:01:11 2014 7, 34 -- Received: from mail-ig0-f181.google.com (mail-ig0-f181.google.com [209.85.213.181]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48F1BPO029837 7, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2014 10:01:11 -0500 7, 34 -- Received: by mail-ig0-f181.google.com with SMTP id h3so2611214igd.8 7, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 08 May 2014 08:01:11 -0700 (PDT) 7, 34 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 34 -- d=1e100.net; s=20130820; 7, 34 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 7, 34 -- :subject:content-type:content-transfer-encoding; 7, 34 -- bh=pVY3w9P6tToRPmYEFGNsuAsIEGG4cp+WOvsOPUs8zTA=; 7, 34 -- b=SyzJ5QofH8lpCxjGRSch69sIpIJvLQFMQzK6i5mretHz9xjyDSwCDTSbA4E4oPlkYi 7, 34 -- Ym47K1oeDSuOrVgS7W6ZIBhlFTEE7qsQdQHS50nH918WZzlkX0DrWY+GwZ9wVTAW8Z6u 7, 34 -- DHtq9Pkpr5hdc8JMJ25Yy14c1BSRUe7iW7+4OAAySWHldpd/0+qXlqWfl9vKkrruGMtv 7, 34 -- YAUh+J4amWYohIig+ne+T3cmQz1R7NtZJ/LcKPA7CHhYJjU6XZExX8WPFzoQoL9brMLd 7, 34 -- R4mFyRdooZzMkJFCryUQoTfnENdFhoJuMRSwKgzuLbfFgpTxuE6zYz6RYL8Gcy+9xqAX 7, 34 -- M7Kw== 7, 34 -- X-Gm-Message-State: ALoCoQmr9O6tybBGPWxdIvo+V4l0B2M8zmd11FVu9Y8DXzU9RHHDcKvrPo1pDpEmTyrQlz2Ebt92 7, 34 -- X-Received: by 10.50.85.37 with SMTP id e5mr9159184igz.43.1399561271244; 7, 34 -- Thu, 08 May 2014 08:01:11 -0700 (PDT) 7, 34 -- Received: from e12-0626-fac.coe.ad.mtu.edu (e12-0626-fac.coe.ad.mtu.edu. [141.219.65.136]) 7, 34 -- by mx.google.com with ESMTPSA id nj14sm6083182igb.18.2014.05.08.08.01.09 7, 34 -- for {Microscopy-at-microscopy.com} 7, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 7, 34 -- Thu, 08 May 2014 08:01:10 -0700 (PDT) 7, 34 -- Message-ID: {536B9C34.8070604-at-mtu.edu} 7, 34 -- Date: Thu, 08 May 2014 11:01:08 -0400 7, 34 -- From: Owen Mills {opmills-at-mtu.edu} 7, 34 -- User-Agent: Postbox 3.0.9 (Macintosh/20140129) 7, 34 -- MIME-Version: 1.0 7, 34 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 34 -- Subject: Need help with EDAX Phoenix problem 7, 34 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 34 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Also, Phil's message suggesting using Bon Ami, damp paper towels and scrubbing is in line with my experience.
I know it is obvious, but do not leave water or cleaner residue in the chamber.
Dan
-----Original Message----- X-from: Crane, Dan - OSHA Sent: Thursday, May 08, 2014 9:00 AM To: 'microscopylistserver-noreply-at-microscopy.com'
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: Univ of Connecticut
Title-Subject: [Filtered] cleaning evaporator
Message: Does anyone have an effective method for cleaning metal deposition from the inside of an evaporator bell-jar?
Cheers Roger Ristau University of Connecticut
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This may not help you with current cleaning but will with subsequent ones....
After the bell jar has been cleaned, we have had good luck using a thin layer of dish soap on the inside of the bell jar which allows the next cleaning to relatively easy as the metal coating will release much easier with warm water. The procedure we use is to clean the bell jar with warm water to remove the prior coating, then using a small drop of dish soap and rubber gloves, smooth it around the inside of the bell jar until it is a white film and allow to dry for a little while. Then replace the bell jar and pump down to outgas... I usually run a "dummy" coating to make sure all is well...
Regards, Scott
Scott A. Payne Assistant Director Electron Microscopy Center North Dakota State University Phone: 701.231.8234
Mailing Address Physical Address/Courier USDA-ARS NCSL, USDA-ARS NCSL 1605 Albrecht Blvd 1307 North 18th St. Fargo ND 58102-2765 Fargo, ND 58102
http://www.ndsu.edu/em_lab/
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Thursday, May 08, 2014 9:31 AM To: Payne, Scott
I use Bon Ami, a non-abrasive kitchen cleaner, a damp paper towel, and scrub. Nothing else.
Phil
} Email: roger.ristau-at-uconn.edu } Name: Roger Ristau } } Organization: Univ of Connecticut } } Title-Subject: [Filtered] cleaning evaporator } } Message: Does anyone have an effective method for cleaning metal } deposition from the inside of an evaporator bell-jar? } } Cheers } Roger Ristau } University of Connecticut -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 29 -- From oshel1pe-at-cmich.edu Thu May 8 09:29:30 2014 3, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48ETU31010337 3, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2014 09:29:30 -0500 3, 29 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 3, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s48ESnn2004473; 3, 29 -- Thu, 8 May 2014 10:29:27 -0400 3, 29 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 3, 29 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 3, 29 -- 14.3.174.1; Thu, 8 May 2014 10:29:09 -0400 3, 29 -- Message-ID: {536B94B7.3040107-at-cmich.edu} 3, 29 -- Date: Thu, 8 May 2014 10:29:11 -0400 3, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 29 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 29 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 29 -- MIME-Version: 1.0 3, 29 -- To: {roger.ristau-at-uconn.edu} , micro {Microscopy-at-microscopy.com} 3, 29 -- Subject: Re: [Microscopy] viaWWW: cleaning evaporator 3, 29 -- References: {201405081419.s48EJd4p006762-at-ns.microscopy.com} 3, 29 -- In-Reply-To: {201405081419.s48EJd4p006762-at-ns.microscopy.com} 3, 29 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- X-Originating-IP: [141.209.160.122] 3, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 29 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 29 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 3, 29 -- X-CanItPRO-Stream: default 3, 29 -- X-Canit-Stats-ID: 02LXqtrRR - 5eaf611ced12 - 20140508 3, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 34 -- From Scott.Payne-at-ndsu.edu Thu May 8 10:24:07 2014 18, 34 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2lp0212.outbound.protection.outlook.com [207.46.163.212]) 18, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48FO7GE004670 18, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2014 10:24:07 -0500 18, 34 -- Received: from BY2PR08MB096.namprd08.prod.outlook.com (10.242.38.147) by 18, 34 -- BY2PR08MB094.namprd08.prod.outlook.com (10.242.38.145) with Microsoft SMTP 18, 34 -- Server (TLS) id 15.0.939.12; Thu, 8 May 2014 15:23:59 +0000 18, 34 -- Received: from BY2PR08MB096.namprd08.prod.outlook.com ([169.254.12.79]) by 18, 34 -- BY2PR08MB096.namprd08.prod.outlook.com ([169.254.12.79]) with mapi id 18, 34 -- 15.00.0939.000; Thu, 8 May 2014 15:23:59 +0000 18, 34 -- From: "Payne, Scott" {Scott.Payne-at-ndsu.edu} 18, 34 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 18, 34 -- Subject: RE: [Microscopy] Re: viaWWW: cleaning evaporator 18, 34 -- Thread-Topic: [Microscopy] Re: viaWWW: cleaning evaporator 18, 34 -- Thread-Index: AQHPaso093YRMQcjhUGBtp0zTCr2/5s2yzcA 18, 34 -- Date: Thu, 8 May 2014 15:23:59 +0000 18, 34 -- Message-ID: {2f434b7dac714fdbbab46fd8a9a4d694-at-BY2PR08MB096.namprd08.prod.outlook.com} 18, 34 -- References: {201405081431.s48EVQ40013363-at-ns.microscopy.com} 18, 34 -- In-Reply-To: {201405081431.s48EVQ40013363-at-ns.microscopy.com} 18, 34 -- Accept-Language: en-US 18, 34 -- Content-Language: en-US 18, 34 -- X-MS-Has-Attach: 18, 34 -- X-MS-TNEF-Correlator: 18, 34 -- x-originating-ip: [2001:4930:124:0:4df9:3236:b74c:c762] 18, 34 -- x-forefront-prvs: 0205EDCD76 18, 34 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(428001)(252514010)(51704005)(13464003)(189002)(199002)(377454003)(33646001)(46102001)(75432001)(74316001)(15202345003)(19580405001)(83322001)(19580395003)(21056001)(54356999)(76176999)(50986999)(76482001)(15975445006)(79102001)(64706001)(20776003)(101416001)(80022001)(77982001)(4396001)(31966008)(512934002)(99286001)(99396002)(74502001)(74662001)(81542001)(2656002)(83072002)(87936001)(86362001)(85852003)(76576001)(92566001)(81342001)(405914003)(3826001)(24736002)(835385003);DIR:OUT;SFP:1101;SCL:1;SRVR:BY2PR08MB094;H:BY2PR08MB096.namprd08.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 18, 34 -- received-spf: None (: ndsu.edu does not designate permitted sender hosts) 18, 34 -- authentication-results: spf=none (sender IP is ) 18, 34 -- smtp.mailfrom=Scott.Payne-at-ndsu.edu; 18, 34 -- Content-Type: text/plain; charset="us-ascii" 18, 34 -- MIME-Version: 1.0 18, 34 -- X-OriginatorOrg: ndsu.edu 18, 34 -- Content-Transfer-Encoding: 8bit 18, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s48FO7GE004670 ==============================End of - Headers==============================
Fullam used to sell a product called "Victawet" that you evaporated onto the surface to make cleaning easier.
Dan
-----Original Message----- X-from: Scott.Payne-at-ndsu.edu [mailto:Scott.Payne-at-ndsu.edu] Sent: Thursday, May 08, 2014 9:37 AM To: Crane, Dan - OSHA
This may not help you with current cleaning but will with subsequent ones....
After the bell jar has been cleaned, we have had good luck using a thin layer of dish soap on the inside of the bell jar which allows the next cleaning to relatively easy as the metal coating will release much easier with warm water. The procedure we use is to clean the bell jar with warm water to remove the prior coating, then using a small drop of dish soap and rubber gloves, smooth it around the inside of the bell jar until it is a white film and allow to dry for a little while. Then replace the bell jar and pump down to outgas... I usually run a "dummy" coating to make sure all is well...
Regards, Scott
Scott A. Payne Assistant Director Electron Microscopy Center North Dakota State University Phone: 701.231.8234
Mailing Address Physical Address/Courier USDA-ARS NCSL, USDA-ARS NCSL 1605 Albrecht Blvd 1307 North 18th St. Fargo ND 58102-2765 Fargo, ND 58102
http://www.ndsu.edu/em_lab/
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Thursday, May 08, 2014 9:31 AM To: Payne, Scott
I use Bon Ami, a non-abrasive kitchen cleaner, a damp paper towel, and scrub. Nothing else.
Phil
} Email: roger.ristau-at-uconn.edu } Name: Roger Ristau } } Organization: Univ of Connecticut } } Title-Subject: [Filtered] cleaning evaporator } } Message: Does anyone have an effective method for cleaning metal } deposition from the inside of an evaporator bell-jar? } } Cheers } Roger Ristau } University of Connecticut -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 29 -- From oshel1pe-at-cmich.edu Thu May 8 09:29:30 2014 3, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48ETU31010337 3, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2014 09:29:30 -0500 3, 29 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 3, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s48ESnn2004473; 3, 29 -- Thu, 8 May 2014 10:29:27 -0400 3, 29 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 3, 29 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 3, 29 -- 14.3.174.1; Thu, 8 May 2014 10:29:09 -0400 3, 29 -- Message-ID: {536B94B7.3040107-at-cmich.edu} 3, 29 -- Date: Thu, 8 May 2014 10:29:11 -0400 3, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 29 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 29 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 29 -- MIME-Version: 1.0 3, 29 -- To: {roger.ristau-at-uconn.edu} , micro {Microscopy-at-microscopy.com} 3, 29 -- Subject: Re: [Microscopy] viaWWW: cleaning evaporator 3, 29 -- References: {201405081419.s48EJd4p006762-at-ns.microscopy.com} 3, 29 -- In-Reply-To: {201405081419.s48EJd4p006762-at-ns.microscopy.com} 3, 29 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- X-Originating-IP: [141.209.160.122] 3, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 29 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 29 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 3, 29 -- X-CanItPRO-Stream: default 3, 29 -- X-Canit-Stats-ID: 02LXqtrRR - 5eaf611ced12 - 20140508 3, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 34 -- From Scott.Payne-at-ndsu.edu Thu May 8 10:24:07 2014 18, 34 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2lp0212.outbound.protection.outlook.com [207.46.163.212]) 18, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48FO7GE004670 18, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2014 10:24:07 -0500 18, 34 -- Received: from BY2PR08MB096.namprd08.prod.outlook.com (10.242.38.147) by 18, 34 -- BY2PR08MB094.namprd08.prod.outlook.com (10.242.38.145) with Microsoft SMTP 18, 34 -- Server (TLS) id 15.0.939.12; Thu, 8 May 2014 15:23:59 +0000 18, 34 -- Received: from BY2PR08MB096.namprd08.prod.outlook.com ([169.254.12.79]) by 18, 34 -- BY2PR08MB096.namprd08.prod.outlook.com ([169.254.12.79]) with mapi id 18, 34 -- 15.00.0939.000; Thu, 8 May 2014 15:23:59 +0000 18, 34 -- From: "Payne, Scott" {Scott.Payne-at-ndsu.edu} 18, 34 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 18, 34 -- Subject: RE: [Microscopy] Re: viaWWW: cleaning evaporator 18, 34 -- Thread-Topic: [Microscopy] Re: viaWWW: cleaning evaporator 18, 34 -- Thread-Index: AQHPaso093YRMQcjhUGBtp0zTCr2/5s2yzcA 18, 34 -- Date: Thu, 8 May 2014 15:23:59 +0000 18, 34 -- Message-ID: {2f434b7dac714fdbbab46fd8a9a4d694-at-BY2PR08MB096.namprd08.prod.outlook.com} 18, 34 -- References: {201405081431.s48EVQ40013363-at-ns.microscopy.com} 18, 34 -- In-Reply-To: {201405081431.s48EVQ40013363-at-ns.microscopy.com} 18, 34 -- Accept-Language: en-US 18, 34 -- Content-Language: en-US 18, 34 -- X-MS-Has-Attach: 18, 34 -- X-MS-TNEF-Correlator: 18, 34 -- x-originating-ip: [2001:4930:124:0:4df9:3236:b74c:c762] 18, 34 -- x-forefront-prvs: 0205EDCD76 18, 34 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(428001)(252514010)(51704005)(13464003)(189002)(199002)(377454003)(33646001)(46102001)(75432001)(74316001)(15202345003)(19580405001)(83322001)(19580395003)(21056001)(54356999)(76176999)(50986999)(76482001)(15975445006)(79102001)(64706001)(20776003)(101416001)(80022001)(77982001)(4396001)(31966008)(512934002)(99286001)(99396002)(74502001)(74662001)(81542001)(2656002)(83072002)(87936001)(86362001)(85852003)(76576001)(92566001)(81342001)(405914003)(3826001)(24736002)(835385003);DIR:OUT;SFP:1101;SCL:1;SRVR:BY2PR08MB094;H:BY2PR08MB096.namprd08.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 18, 34 -- received-spf: None (: ndsu.edu does not designate permitted sender hosts) 18, 34 -- authentication-results: spf=none (sender IP is ) 18, 34 -- smtp.mailfrom=Scott.Payne-at-ndsu.edu; 18, 34 -- Content-Type: text/plain; charset="us-ascii" 18, 34 -- MIME-Version: 1.0 18, 34 -- X-OriginatorOrg: ndsu.edu 18, 34 -- Content-Transfer-Encoding: 8bit 18, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s48FO7GE004670 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 59 -- From Crane.Dan-at-dol.gov Thu May 8 10:44:16 2014 26, 59 -- Received: from sil-mta-dmz-01-pub.dol.gov (sil-mta-dmz-01-pub.dol.gov [152.180.11.244]) 26, 59 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48FiGsB010518 26, 59 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2014 10:44:16 -0500 26, 59 -- X-SBRS: None 26, 59 -- X-HAT-SENDERGROUP: CES_OUTBOUND 26, 59 -- X-HAT-SENDER_IP: 10.48.33.11 26, 59 -- X-RCPT-TO: Microscopy-at-microscopy.com 26, 59 -- X-IronPort-Anti-Spam-Filtered: true 26, 59 -- X-IronPort-Anti-Spam-Result: ApcJAJKla1MKMCEL/2dsb2JhbABWAw6CeE9YxVsEAgGBExZ0giUBAQQBOhQgCwUHAgICAQUDEQMBAQENEgkHGwQSARQJCAIECgQFCBUEiAwDCQgNrVibdRiGPRcEjEIKgSAQAgEkGxACBQYLhC4EhFoCbYZYgyKLMYthhV6CdkA9MAGBQQ 26, 59 -- X-IPAS-Result: ApcJAJKla1MKMCEL/2dsb2JhbABWAw6CeE9YxVsEAgGBExZ0giUBAQQBOhQgCwUHAgICAQUDEQMBAQENEgkHGwQSARQJCAIECgQFCBUEiAwDCQgNrVibdRiGPRcEjEIKgSAQAgEkGxACBQYLhC4EhFoCbYZYgyKLMYthhV6CdkA9MAGBQQ 26, 59 -- X-IronPort-AV: E=Sophos;i="4.97,1010,1389762000"; 26, 59 -- d="scan'208";a="3534398" 26, 59 -- Received: from sil-mta-int-01-ext.dol.gov ([10.48.33.11]) 26, 59 -- by sil-mta-dmz-01-int.dol.gov with ESMTP; 08 May 2014 11:44:15 -0400 26, 59 -- X-HAT-DCN-SENDERGROUP: CES_OUTBOUND 26, 59 -- X-IronPort-Anti-Spam-Filtered: true 26, 59 -- X-IronPort-Anti-Spam-Result: AiUFAB6la1MKMB4o/2dsb2JhbABWAw6CeE9YxWEBgRMWdIIlAQEEAToUIAsFBwICAgEFAxEDAQEBDRIJBxsEEgEUCQgCBAoEBQgVBIgMAwkIDa1Wm3UYhj0XBIxCCoEgEAIBJBsQAgUGC4QuBIRaAm2GWIMiizGLYYVegnZAPTABgUE 26, 59 -- X-IPAS-Result: AiUFAB6la1MKMB4o/2dsb2JhbABWAw6CeE9YxWEBgRMWdIIlAQEEAToUIAsFBwICAgEFAxEDAQEBDRIJBxsEEgEUCQgCBAoEBQgVBIgMAwkIDa1Wm3UYhj0XBIxCCoEgEAIBJBsQAgUGC4QuBIRaAm2GWIMiizGLYYVegnZAPTABgUE 26, 59 -- X-IronPort-AV: E=Sophos;i="4.97,1010,1389762000"; 26, 59 -- d="scan'208";a="1264910" 26, 59 -- Received: from silexht01.oasam.dir.labor.gov ([10.48.30.40]) 26, 59 -- by sil-mta-int-01-int.dol.gov with ESMTP; 08 May 2014 11:44:14 -0400 26, 59 -- Received: from M2-EXCH01.oasam.dir.labor.gov (10.48.27.130) by 26, 59 -- SILEXHT01.oasam.dir.labor.gov (10.48.30.40) with Microsoft SMTP Server (TLS) 26, 59 -- id 14.3.169.1; Thu, 8 May 2014 11:44:14 -0400 26, 59 -- Received: from na01-by2-obe.outbound.protection.outlook.com (10.49.5.10) by 26, 59 -- dol.gov (10.48.27.130) with Microsoft SMTP Server (TLS) id 14.3.181.6; Thu, 8 26, 59 -- May 2014 11:44:14 -0400 26, 59 -- Received: from BLUPR09MB101.namprd09.prod.outlook.com (10.255.212.140) by 26, 59 -- BLUPR09MB103.namprd09.prod.outlook.com (10.255.212.23) with Microsoft SMTP 26, 59 -- Server (TLS) id 15.0.939.12; Thu, 8 May 2014 15:44:11 +0000 26, 59 -- Received: from BLUPR09MB101.namprd09.prod.outlook.com ([10.255.212.140]) by 26, 59 -- BLUPR09MB101.namprd09.prod.outlook.com ([10.255.212.140]) with mapi id 26, 59 -- 15.00.0939.000; Thu, 8 May 2014 15:44:11 +0000 26, 59 -- From: "Crane, Dan - OSHA" {Crane.Dan-at-dol.gov} 26, 59 -- To: "Scott.Payne-at-ndsu.edu" {Scott.Payne-at-ndsu.edu} 26, 59 -- CC: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 26, 59 -- Subject: RE: [Microscopy] viaWWW: cleaning evaporator 26, 59 -- Thread-Topic: [Microscopy] viaWWW: cleaning evaporator 26, 59 -- Thread-Index: AQHPatOUdjyCH+iY8kCn3V0u1NsWQ5s20qUw 26, 59 -- Date: Thu, 8 May 2014 15:44:10 +0000 26, 59 -- Message-ID: {b8a7b259094f43e7af4f51b30e4f639d-at-BLUPR09MB101.namprd09.prod.outlook.com} 26, 59 -- References: {201405081536.s48Favco004926-at-ns.microscopy.com} 26, 59 -- In-Reply-To: {201405081536.s48Favco004926-at-ns.microscopy.com} 26, 59 -- Accept-Language: en-US 26, 59 -- Content-Language: en-US 26, 59 -- X-MS-Has-Attach: 26, 59 -- X-MS-TNEF-Correlator: 26, 59 -- x-originating-ip: [152.180.134.66] 26, 59 -- x-forefront-prvs: 0205EDCD76 26, 59 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009001)(6009001)(428001)(377454003)(51704005)(189002)(199002)(13464003)(252514010)(87936001)(83322001)(19580405001)(19580395003)(15975445006)(79102001)(101416001)(2656002)(80022001)(21056001)(66066001)(2171001)(85852003)(74316001)(31966008)(81342001)(92566001)(46102001)(74502001)(77096999)(74662001)(54356999)(83072002)(76176999)(50986999)(15202345003)(575784001)(77982001)(86362001)(64706001)(76482001)(99286001)(99396002)(33646001)(20776003)(512934002)(81542001)(76576001)(512954002)(4396001)(405914003)(24736002)(835385003);DIR:OUT;SFP:1101;SCL:1;SRVR:BLUPR09MB103;H:BLUPR09MB101.namprd09.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 26, 59 -- authentication-results: spf=none (sender IP is ) 26, 59 -- smtp.mailfrom=Crane.Dan-at-dol.gov; 26, 59 -- Content-Type: text/plain; charset="us-ascii" 26, 59 -- MIME-Version: 1.0 26, 59 -- X-OriginatorOrg: dol.gov 26, 59 -- Content-Transfer-Encoding: 8bit 26, 59 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s48FiGsB010518 ==============================End of - Headers==============================
I agree that a thin layer of detergent is good, but my experience is that dish-washing detergent is better than dish soap.
And this gives me the opportunity to get off track for a moment. The main purpose of shaving foam is to make the skin wet. So I shave after showering with no shaving soap - it works just fine. However the mirror tends to get steamed up during the shower. The solution to this problem is to apply a little dish-washing fluid to the mirror. Then it does not steam up. -- Alwyn Eades Department of Materials Science and Engineering Lehigh University
==============================Original Headers============================== 3, 20 -- From jae5-at-lehigh.edu Thu May 8 10:57:10 2014 3, 20 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48FvAnk029660 3, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2014 10:57:10 -0500 3, 20 -- Received: from [192.168.1.117] (24.102.248.186.res-cmts.hel.ptd.net [24.102.248.186]) 3, 20 -- (authenticated bits=0) 3, 20 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id s48Fv2QA002554 3, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT) 3, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2014 11:57:09 -0400 3, 20 -- Message-ID: {536BA947.5090208-at-lehigh.edu} 3, 20 -- Date: Thu, 08 May 2014 11:56:55 -0400 3, 20 -- From: Alwyn Eades {jae5-at-lehigh.edu} 3, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 3, 20 -- MIME-Version: 1.0 3, 20 -- To: Microscopy-at-microscopy.com 3, 20 -- Subject: cleaning evaporator 3, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-Virus-Scanned: clamav-milter 0.98 at rain.cc.lehigh.edu 3, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I spray the inside of the bell jar or cylinder with unscented White Rain hair spray. Warm water will dissolve the hairspray and release the metal, whether evaporated or sputtered. Using unscented avoids unneeded volatiles in the vacuum system.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: jae5-at-lehigh.edu [mailto:jae5-at-lehigh.edu] Sent: Thursday, May 08, 2014 11:59 AM To: kenconverse-at-qualityimages.biz
I agree that a thin layer of detergent is good, but my experience is that dish-washing detergent is better than dish soap.
And this gives me the opportunity to get off track for a moment. The main purpose of shaving foam is to make the skin wet. So I shave after showering with no shaving soap - it works just fine. However the mirror tends to get steamed up during the shower. The solution to this problem is to apply a little dish-washing fluid to the mirror. Then it does not steam up. -- Alwyn Eades Department of Materials Science and Engineering Lehigh University
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==============================Original Headers============================== 15, 27 -- From kenconverse-at-qualityimages.biz Thu May 8 13:01:58 2014 15, 27 -- Received: from cdptpa-oedge-vip.email.rr.com (cdptpa-outbound-snat.email.rr.com [107.14.166.226]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48I1w7T004878 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2014 13:01:58 -0500 15, 27 -- Received: from [108.183.173.180] ([108.183.173.180:1187] helo=Ken) 15, 27 -- by cdptpa-oedge03 (envelope-from {kenconverse-at-qualityimages.biz} ) 15, 27 -- (ecelerity 3.5.0.35861 r(Momo-dev:tip)) with ESMTP 15, 27 -- id 96/92-15876-596CB635; Thu, 08 May 2014 18:01:57 +0000 15, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 15, 27 -- To: "MSA Listserver" {microscopy-at-microscopy.com} 15, 27 -- Subject: RE: [Microscopy] cleaning evaporator 15, 27 -- Date: Thu, 8 May 2014 14:02:48 -0400 15, 27 -- Message-ID: {D669CDD7BFCE4C509C863402DD66DEFB-at-Ken} 15, 27 -- MIME-Version: 1.0 15, 27 -- Content-Type: text/plain; 15, 27 -- charset="us-ascii" 15, 27 -- X-Priority: 3 (Normal) 15, 27 -- X-MSMail-Priority: Normal 15, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 15, 27 -- Thread-Index: Ac9q1m61s5gnDKLNS/SZEMX69WJaOgAEL9Mg 15, 27 -- Importance: Normal 15, 27 -- In-Reply-To: {201405081558.s48FwnGI032711-at-ns.microscopy.com} 15, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 15, 27 -- X-RR-Connecting-IP: 107.14.168.142:25 15, 27 -- X-Cloudmark-Score: 0 15, 27 -- Content-Transfer-Encoding: 8bit 15, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s48I1w7T004878 ==============================End of - Headers==============================
From pharmacy_canadian7-at-airtelbroadband.in Thu May 8 13:38:58 2014 Return-Path: {pharmacy_canadian7-at-airtelbroadband.in} Received: from ABTS-KK-dynamic-048.164.166.122.airtelbroadband.in (ABTS-KK-dynamic-048.164.166.122.airtelbroadband.in [122.166.164.48] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s48Icsb8024505 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 8 May 2014 13:38:55 -0500 Message-Id: {201405081838.s48Icsb8024505-at-ns.microscopy.com}
We use Jif (known as Cif in some places). It is a non-abrasive cleaner probably exactly like Bon Ami.
After years (decades in my lab's history) of using Wenol to clean parts of our TEM gun etc., we were first surprised then tried it on the advice of a senior FEI engineer.
The main ingredients are calcium carbonate particles and a couple of surfactants. As long as the scented versions are avoided, it leaves no residue if rinsed properly since all ingredients are very water soluble.
Duane
Duane Harland, Agresearch, New Zealand
________________________________________ X-from: oshel1pe-at-cmich.edu [oshel1pe-at-cmich.edu] Sent: 09 May 2014 02:39 To: Harland, Duane
I use Bon Ami, a non-abrasive kitchen cleaner, a damp paper towel, and scrub. Nothing else.
Phil
} Email: roger.ristau-at-uconn.edu } Name: Roger Ristau } } Organization: Univ of Connecticut } } Title-Subject: [Filtered] cleaning evaporator } } Message: Does anyone have an effective method for cleaning metal deposition from the inside of an } evaporator bell-jar? } } Cheers } Roger Ristau } University of Connecticut -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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Email: willis.robert-at-epa.gov Name: Bob Willis
Organization: US EPA
Title-Subject: [Filtered] Bruker EDS-Tescan interface problem
Message: I have a Quantax EDS system attached to a Tescan MIRA3 FE-SEM. The two PCs are communicating with each other, but the Quantax is not able to take control of the SEM or read SEM parameters (mag, HV, etc). It is capable of collecting an image if the SEM is put in external scanning mode.
Thanks for any suggestions.
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Email: roger.ristau-at- uconn.edu Name: Roger Ristau
Organization: Univ of Connecticut
Title-Subject: [Filtered] cleaning evaporator
Message: Who would have thought that such an innocent question would bring so many interesting solutions. Thanks to all for great ideas. If the improvement in my results is as good as suggested, I may quit my day job and go into the "window washing" business!
Cheers Roger Ristau University of Connecticut
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It has been a long time since I cleaned a bell jar, but I seem to recall using Bon Ami, as others suggest. However, to facilitate removal of subsequently deposited materials I seem to remember evaporating a fair size piece of rock salt onto the inside surface right after cleaning. This is a non-organic material with low vapor pressure, and, as I recall, did a pretty good job as a release agent for the next cleaning, -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 32 -- From bigelow-at-umich.edu Thu May 8 22:48:02 2014 1, 32 -- Received: from mail-ig0-f172.google.com (mail-ig0-f172.google.com [209.85.213.172]) 1, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s493m2Z2004151 1, 32 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2014 22:48:02 -0500 1, 32 -- Received: by mail-ig0-f172.google.com with SMTP id uy17so655940igb.5 1, 32 -- for {microscopy-at-microscopy.com} ; Thu, 08 May 2014 20:48:01 -0700 (PDT) 1, 32 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 32 -- d=1e100.net; s=20130820; 1, 32 -- h=x-gm-message-state:mime-version:message-id:date:to:from:subject 1, 32 -- :content-type; 1, 32 -- bh=QhsQ0hU6uzF34wBh7K7J9kvRZBA6Et/ptxTnqFVGI1k=; 1, 32 -- b=mcMjsxpncnVTcLF8v6WT+Pjkl6xTMThlb6/KWgVVZtmY/MJ/I1ylObmUGi7r4vRL71 1, 32 -- E2r1U2SK5lX0iCZHOrj1y078JN1vPRcIyYChsGv8B2tQo+wZ04CTXiiry2KB7WFdcycf 1, 32 -- PaMaeJTtURR4WJhPFwGE6SX/Go5JmJhKFjy1dRdjts9lSQ2DhUU3gNR7grRLu1C6PLEH 1, 32 -- oBaP4xw4bz6IlGi6yEV5wv6Pa3UYkQng4z1IT2CyYI9FKFbxnU4OIvyaYUjV/gDlX5Fj 1, 32 -- PFQVgwB5zJGpGz/YV0H0QoQ1JJRS2LDWc+k3F83iTkvNyZJ33ZBm3YCeb+grkEwgNo6F 1, 32 -- aJ0Q== 1, 32 -- X-Gm-Message-State: ALoCoQlmZ+O3O5LMGIurHcweAnqY8E1yk1MVa/oLqWaBlX2CCngSiJ/0Uh4KGQwMBKftshoNWcjC 1, 32 -- X-Received: by 10.50.25.136 with SMTP id c8mr3830787igg.26.1399607281870; 1, 32 -- Thu, 08 May 2014 20:48:01 -0700 (PDT) 1, 32 -- Received: from [76.226.115.171] ([76.226.115.171]) 1, 32 -- by mx.google.com with ESMTPSA id p4sm1103750igy.7.2014.05.08.20.47.58 1, 32 -- for {microscopy-at-microscopy.com} 1, 32 -- (version=TLSv1 cipher=RC4-SHA bits=128/128); 1, 32 -- Thu, 08 May 2014 20:48:00 -0700 (PDT) 1, 32 -- Mime-Version: 1.0 1, 32 -- Message-Id: {p06240802cf91fead7043-at-[76.226.115.171]} 1, 32 -- Date: Thu, 8 May 2014 23:47:31 -0400 1, 32 -- To: Microscopy {microscopy-at-microscopy.com} 1, 32 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 32 -- Subject: [Microscopy]Cleaning 1, 32 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Hi All If you are interested in Outreach and putting on a microscopy workshop at a local school or community then you will be interested in the programs on offer this year at the M&M meeting in Hartford in August. Microscopy in the Classroom has a number of talks by experienced speakers and ProjectMICRO is putting on a hands-on workshop for delegates and teachers. All participants will take away experiences and ideas to enhance their program. I have put flyers for these two sessions in a dropbox that you can download at https://www.dropbox.com/sh/fkyls0q5jny69ah/AACrHMOijcXm6fF9EXbLYtP7a
Also we are planning another Family Affair for delegates families and friends. Each year we try to bring your favourites and something different. This year....
The story so far A secret agent, James B Atom, wrote a message very very very small. The first part of the message can be read with a light microscope. The second part of the message can only be read using a scanning electron microscope. The message gets split up and passed on and part comes to you. Your mission, should you take it, is to decipher the message, and bring it to headquarters (the Outreach booth). To assist you in this, we have the use of the devices needed in the classroom and the Exhibition Hall.
Hope to see you all in August Elaine
Dr. Elaine C. Humphrey STEHM Technologist and Lab Manager Bob Wright Science Centre A015 Advanced Microscopy Facility University of Victoria, Canada
==============================Original Headers============================== 12, 32 -- From ech-at-uvic.ca Fri May 9 10:44:45 2014 12, 32 -- Received: from mamba.comp.uvic.ca (mamba.comp.uvic.ca [142.104.47.36]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s49FiioN004751 12, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 9 May 2014 10:44:45 -0500 12, 32 -- Received: from LONGFIN.uvic.ca (longfin.uvic.ca [142.104.240.132]) 12, 32 -- by mamba.comp.uvic.ca (8.14.4/8.14.4) with ESMTP id s49FiRjx028534 12, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 9 May 2014 08:44:39 -0700 12, 32 -- Received: from BURI.uvic.ca ([fe80::989c:e3cc:730f:e087]) by LONGFIN.uvic.ca 12, 32 -- ([fe80::200:5efe:142.104.240.132%11]) with mapi id 14.03.0174.001; Fri, 9 May 12, 32 -- 2014 08:44:33 -0700 12, 32 -- From: Elaine Humphrey {ech-at-uvic.ca} 12, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 32 -- Subject: Outreach at M&M 2014 12, 32 -- Thread-Topic: Outreach at M&M 2014 12, 32 -- Thread-Index: AQHPa52RIN6SWYb+wEqseJZM7aQ7mA== 12, 32 -- Date: Fri, 9 May 2014 15:44:31 +0000 12, 32 -- Message-ID: {CF924313.245B5%ech-at-uvic.ca} 12, 32 -- Accept-Language: en-US 12, 32 -- Content-Language: en-US 12, 32 -- X-MS-Has-Attach: 12, 32 -- X-MS-TNEF-Correlator: 12, 32 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 12, 32 -- x-originating-ip: [142.104.193.193] 12, 32 -- Content-Type: text/plain; charset="us-ascii" 12, 32 -- Content-ID: {10ECC3DC19C8D2468C7EF3BD4F4B7EE2-at-local.uvic.ca} 12, 32 -- MIME-Version: 1.0 12, 32 -- X-UVic-Virus-Scanned: OK - Passed virus scan by ClamAV (clamd) on mamba 12, 32 -- X-UVic-Virus-Scanned: OK - Passed virus scan by Sophos (sophie) on mamba 12, 32 -- X-UVic-Scan: mamba.comp.uvic.ca filter_version 3.7.7 12, 32 -- X-Scanned-By: MIMEDefang 2.67 on 142.104.47.37 12, 32 -- Content-Transfer-Encoding: 8bit 12, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s49FiioN004751 ==============================End of - Headers==============================
From affordable-medications1-at-puzzle.su Fri May 9 12:11:10 2014 Return-Path: {affordable-medications1-at-puzzle.su} Received: from 7142dfd8b278428.orel.puzzle.su (ip-46-72-104-162.bb.netbynet.ru [46.72.104.162] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s49HB2rM024834 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 9 May 2014 12:11:08 -0500 Message-Id: {201405091711.s49HB2rM024834-at-ns.microscopy.com}
For those of you who do color brightfield, this might be an interesting seminar.
"Achieving Color Integrity in Brightfield Images: A Color Science Method for Color Calibration" When: Tuesday, May 13, 2014 -at- 10:00am Where: Health Science Research Facility, University of Vermont College of Medicine, Burlington, VT
Hosted by the Microscopy Imaging Center, Datacolor will present a seminar at the University of Vermont College of Medicine that reviews opportunities to maintain color integrity in brightfield imaging, beginning with sources of color variability and offering a new approach to making color brightfield images publication ready without using Photoshop.
Abstract: Transmitted light brightfield microscope images reveal the impact of an enormous number of color variables outside the specimen itself. This seminar will review the sources of many of these variables, and discuss mitigating approaches to controlling color variability in brightfield images. A novel color science-based solution offers an empirical approach to calibrating images to a standard for greater consistency, comparability and efficiency than existing methods, from image capture through display. Additionally, it automatically records the audit trail, a considerable advantage when presenting images to reviewers.
For further details, visit www.ChromaCal.com
Best regards, Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through Fall 2014. Call us today for a free training evaluation.
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Email: gary-at-gaugler.com Name: Dr Gary Gaugler
Organization: Microtechnics, Inc.
Title-Subject: [Filtered] FEI Quanta 600 ESEM
Message: Greetings. I'm considering purchasing a Quanta ESEM (gen 1, W) and wonder if there are downsides to this for general SEM work? Usually I have done high vac imaging using SFEG SEMs. The Quanta looks to be really different from high vac only tools and also from variable pressure tools. Can it really do a good job of different specimens and in different modes up to about 100KX? Would appreciate hearing from any users about hints and experiences.
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==============================Original Headers============================== 9, 29 -- From microscopylistserver-noreply-at-microscopy.com Fri May 9 17:12:24 2014 9, 29 -- Received: from znl.com ([206.69.208.20]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s49MCONM014409 9, 29 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2014 17:12:24 -0500 9, 29 -- Received: from localhost (localhost [127.0.0.1]) 9, 29 -- by znl.com (Postfix) with ESMTP id 6DCC36FCD12 9, 29 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2014 17:12:24 -0500 (CDT) 9, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 9, 29 -- Received: from znl.com ([127.0.0.1]) 9, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 9, 29 -- with ESMTP id U-XaSPwEImQw for {microscopy-at-microscopy.com} ; 9, 29 -- Fri, 9 May 2014 17:12:16 -0500 (CDT) 9, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 9, 29 -- by znl.com (Postfix) with ESMTPA id 30DD96FCD05 9, 29 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2014 17:12:16 -0500 (CDT) 9, 29 -- Message-ID: {536D52BF.9010806-at-microscopy.com} 9, 29 -- Date: Fri, 09 May 2014 17:12:15 -0500 9, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 9, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 9, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 9, 29 -- MIME-Version: 1.0 9, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} , 9, 29 -- MicroscopyListServer-Forward {microscopy-at-microscopy.com} 9, 29 -- Subject: viaWWW:FEI Quanta 600 ESEM 9, 29 -- References: {201405091613.s49GD0TS024144-at-ns.microscopy.com} 9, 29 -- In-Reply-To: {201405091613.s49GD0TS024144-at-ns.microscopy.com} 9, 29 -- X-Forwarded-Message-Id: {201405091613.s49GD0TS024144-at-ns.microscopy.com} 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Microscopy Society of Northeastern Ohio, MSNO
Title-Subject: [Filtered] Spring MSNO meeting - May 21st Meeting at John Carroll University
Message: Spring MSNO meeting - May 21st Meeting at John Carroll University
We have updated our website with the full program information. http://www.msneo.org/meetings.html If you plan to join please register ASAP.
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri May 9 17:12:52 2014 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s49MCpQE014654 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2014 17:12:51 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id ECC2C6FCD20 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2014 17:12:51 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id MgNXTPyiQvd5 for {microscopy-at-microscopy.com} ; 11, 28 -- Fri, 9 May 2014 17:12:48 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 3A5C26FCD13 11, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2014 17:12:48 -0500 (CDT) 11, 28 -- Message-ID: {536D52E0.9030300-at-microscopy.com} 11, 28 -- Date: Fri, 09 May 2014 17:12:48 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: Fwd: [Filtered] MicroscopyListserverviaWWW: 11, 28 -- References: {201405091624.s49GOkoU024302-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201405091624.s49GOkoU024302-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201405091624.s49GOkoU024302-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We replaced a venerable JEOL 840A (W-gun) with a FEI Quanta FEG 250 about 4 years ago. We have not been disappointed.
For several years we had been imaging nanoparticles at 50kx on the 840 and figured we really needed to make the switch to a field emission gun to get better resolution. Most application engineers told us we were doing quite well considering our W gun, but there was no doubt about the performance improvement to come from the FE gun (1 nm vws. 3 nm resolution).
I suppose the Quanta with tungsten gun should be good, but it may be a stretch to get satisfying images at 100kx. If you want, I can send you some comparison images.
Of course, even though we can reach 100kx rather easily on the Quanta-FEG, our users also spend a lot of time below 1kx. (I have to wonder why they are using this scope instead of a W alternative.)
Warren ________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com {microscopylistserver-noreply-at-microscopy.com} Sent: Friday, May 09, 2014 5:13 PM To: Straszheim, Warren E [BIOTC]
X-from: gary-at-gaugler.com ()
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Email: gary-at-gaugler.com Name: Dr Gary Gaugler
Organization: Microtechnics, Inc.
Title-Subject: [Filtered] FEI Quanta 600 ESEM
Message: Greetings. I'm considering purchasing a Quanta ESEM (gen 1, W) and wonder if there are downsides to this for general SEM work? Usually I have done high vac imaging using SFEG SEMs. The Quanta looks to be really different from high vac only tools and also from variable pressure tools. Can it really do a good job of different specimens and in different modes up to about 100KX? Would appreciate hearing from any users about hints and experiences.
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==============================Original Headers============================== 17, 35 -- From wesaia-at-iastate.edu Fri May 9 17:39:41 2014 17, 35 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0182.outbound.protection.outlook.com [207.46.163.182]) 17, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s49Mdbe9014386 17, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 9 May 2014 17:39:40 -0500 17, 35 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 17, 35 -- BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) with Microsoft SMTP 17, 35 -- Server (TLS) id 15.0.934.12; Fri, 9 May 2014 22:39:36 +0000 17, 35 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.48]) by 17, 35 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.48]) with mapi id 17, 35 -- 15.00.0934.000; Fri, 9 May 2014 22:39:36 +0000 17, 35 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 17, 35 -- To: MSA {Microscopy-at-microscopy.com} 17, 35 -- CC: "gary-at-gaugler.com" {gary-at-gaugler.com} 17, 35 -- Subject: RE: [Microscopy] viaWWW:FEI Quanta 600 ESEM 17, 35 -- Thread-Topic: [Microscopy] viaWWW:FEI Quanta 600 ESEM 17, 35 -- Thread-Index: AQHPa9PwczIZqk9qHUyDUlQbMpo6mZs41Y0b 17, 35 -- Date: Fri, 9 May 2014 22:39:35 +0000 17, 35 -- Message-ID: {1399675175296.47159-at-iastate.edu} 17, 35 -- References: {201405092213.s49MDOph016000-at-ns.microscopy.com} 17, 35 -- In-Reply-To: {201405092213.s49MDOph016000-at-ns.microscopy.com} 17, 35 -- Accept-Language: en-US 17, 35 -- Content-Language: en-US 17, 35 -- X-MS-Has-Attach: 17, 35 -- X-MS-TNEF-Correlator: 17, 35 -- x-originating-ip: [129.186.227.19] 17, 35 -- x-forefront-prvs: 02065A9E77 17, 35 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(428001)(64544002)(189002)(199002)(377454003)(252514010)(86362001)(101416001)(83322001)(21056001)(575784001)(79102001)(15202345003)(19580395003)(15395725003)(19580405001)(74662001)(74502001)(81542001)(81342001)(50986999)(31966008)(54356999)(99396002)(83072002)(66066001)(85852003)(2656002)(80022001)(20776003)(64706001)(87936001)(76176999)(77982001)(99286001)(15975445006)(46102001)(76482001)(92726001)(92566001)(36756003)(75432001)(4396001);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR04MB546;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 17, 35 -- received-spf: None (: iastate.edu does not designate permitted sender hosts) 17, 35 -- authentication-results: spf=none (sender IP is ) 17, 35 -- smtp.mailfrom=wesaia-at-iastate.edu; 17, 35 -- Content-Type: text/plain; charset="us-ascii" 17, 35 -- MIME-Version: 1.0 17, 35 -- X-OriginatorOrg: iastate.edu 17, 35 -- Content-Transfer-Encoding: 8bit 17, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s49Mdbe9014386 ==============================End of - Headers==============================
From best-drugs15-at-td-magnit.net Fri May 9 22:14:35 2014 Return-Path: {best-drugs15-at-td-magnit.net} Received: from serv.zabba.td-magnit.net ([91.207.218.40]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4A3EUrh008882 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 9 May 2014 22:14:30 -0500 Message-Id: {201405100314.s4A3EUrh008882-at-ns.microscopy.com}
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Message: Dear Listers, We are working to get ISO 17025 accreditation for our scanning electron microscopy laboratory. I do appreciate so much if someone who reached the accreditation can help us with the sample selection to calculate the accuracy of the method. Thanks you in advance, Yours, Patricia
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From reasonable_pharmacy14-at-nordstrom.com Mon May 12 03:44:07 2014 Return-Path: {reasonable_pharmacy14-at-nordstrom.com} Received: from smtp2.nordstrom.com ([62.76.98.9]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4C8i4p6026052 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 12 May 2014 03:44:05 -0500 Message-Id: {201405120844.s4C8i4p6026052-at-ns.microscopy.com} X-AntiVirus: Checked by Dr.Web [version: 8.0.7.07121, engine: 8.3.0.10280, virus records: 4945313, updated: 15.02.2014]
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Professor James Whisstock ARC Federation Fellow Department of Biochemistry Monash University Clayton, Melbourne, VIC, 3800 Australia Email: james.whisstock-at-monash.edu
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==============================Original Headers============================== 16, 29 -- From microscopylistserver-noreply-at-microscopy.com Mon May 12 06:44:17 2014 16, 29 -- Received: from znl.com ([206.69.208.20]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4CBiHgV028794 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 06:44:17 -0500 16, 29 -- Received: from localhost (localhost [127.0.0.1]) 16, 29 -- by znl.com (Postfix) with ESMTP id 929C36FE839 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 06:44:17 -0500 (CDT) 16, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 29 -- Received: from znl.com ([127.0.0.1]) 16, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 29 -- with ESMTP id 4xEOKPWM6lTU for {microscopy-at-microscopy.com} ; 16, 29 -- Mon, 12 May 2014 06:44:10 -0500 (CDT) 16, 29 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 16, 29 -- by znl.com (Postfix) with ESMTPA id C3D1A6FE827 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 06:44:10 -0500 (CDT) 16, 29 -- Message-ID: {5370B40A.4060200-at-microscopy.com} 16, 29 -- Date: Mon, 12 May 2014 06:44:10 -0500 16, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 16, 29 -- MIME-Version: 1.0 16, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 29 -- Subject: viaWWW:Professor of Structural Cryo-Electron Microscopy - position 16, 29 -- available 16, 29 -- References: {201405121000.s4CA0RH8027303-at-ns.microscopy.com} 16, 29 -- In-Reply-To: {201405121000.s4CA0RH8027303-at-ns.microscopy.com} 16, 29 -- X-Forwarded-Message-Id: {201405121000.s4CA0RH8027303-at-ns.microscopy.com} 16, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would be interested in this information too so if you wouldn't mind replying to me as well or to the board it would be greatly appreciated. Thanks.
Sincerely,
James Neal-Kababick Director Flora Research Laboratories An FDA and DEA Registered & Inspected Laboratory Fellow AOAC International Adjunct Faculty Bastyr University Botanical Medicine Department 1-541-472-0980 phone jimk-at-floraresearch.com www.floraresearch.com
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-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Sunday, May 11, 2014 7:42 PM To: James Neal-Kababick
X-from: patricia.bozzano-at-gmail.com ()
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Message: Dear Listers, We are working to get ISO 17025 accreditation for our scanning electron microscopy laboratory. I do appreciate so much if someone who reached the accreditation can help us with the sample selection to calculate the accuracy of the method. Thanks you in advance, Yours, Patricia
==============================Original Headers============================== 19, 30 -- From jimk-at-floraresearch.com Mon May 12 12:52:54 2014 19, 30 -- Received: from xsmtp07.mail2web.com (xsmtp07.mail2web.com [168.144.250.170]) 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4CHqroZ021712 19, 30 -- for {Microscopy-at-Microscopy.com} ; Mon, 12 May 2014 12:52:53 -0500 19, 30 -- Received: from [10.5.31.9] (helo=hub03.ad2.softcom.biz) 19, 30 -- by xsmtp07.mail2web.com with esmtps (TLS-1.0:RSA_ARCFOUR_MD5:16) 19, 30 -- (Exim 4.63) 19, 30 -- (envelope-from {jimk-at-floraresearch.com} ) 19, 30 -- id 1WjuPB-00084X-Tl 19, 30 -- for Microscopy-at-Microscopy.com; Mon, 12 May 2014 13:52:53 -0400 19, 30 -- Received: from MBX74.ad2.softcom.biz ([10.5.13.37]) by hub03.ad2.softcom.biz 19, 30 -- ([10.5.31.9]) with mapi; Mon, 12 May 2014 13:52:49 -0400 19, 30 -- From: James Neal-Kababick {jimk-at-floraresearch.com} 19, 30 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 19, 30 -- Date: Mon, 12 May 2014 13:52:49 -0400 19, 30 -- Thread-Topic: [Microscopy] viaWWW:ISO 17025 Acreditation 19, 30 -- Thread-Index: Ac9ti7OgfE9SGPxfTZWbNv5tVTgPewAfe5ug 19, 30 -- Message-ID: {143B8CF2094404458B4AA679F5A9877B6D9A674F2F-at-MBX74.ad2.softcom.biz} 19, 30 -- References: {201405120241.s4C2fYMx023097-at-ns.microscopy.com} 19, 30 -- In-Reply-To: {201405120241.s4C2fYMx023097-at-ns.microscopy.com} 19, 30 -- Accept-Language: en-US 19, 30 -- Content-Language: en-US 19, 30 -- X-MS-Has-Attach: 19, 30 -- X-MS-TNEF-Correlator: 19, 30 -- acceptlanguage: en-US 19, 30 -- Content-Type: text/plain; charset="us-ascii" 19, 30 -- MIME-Version: 1.0 19, 30 -- Subject: RE: [Microscopy] viaWWW:ISO 17025 Acreditation 19, 30 -- Content-Transfer-Encoding: 8bit 19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4CHqroZ021712 ==============================End of - Headers==============================
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Email: pwebster-at-usc.edu Name: Paul Webster
Organization: University of Southern California
Title-Subject: [Filtered] Third Party Service for EM
Message: Dear All,
I know this subject comes up often, and I have the same feelings about the subject that are often expressed on this server. However I can't find the material in the archives. My apologies in advance for this question.
So, here goes: Does anyone have any experiences with service being provided through third party companies such as the Remi Group.
Past replies have highlighted the lack of specific support for electron microscopes, the difficulty in getting priority service, the need to pay for parts etc.
Do these concerns still apply or have the third party providers improved their service? Comments from anyone who is or has been covered by such a company will be welcome, however,replies from anyone with an opinion are encouraged (positive and negative). We are preparing our reply to our bean counters.
Best regards,
Paul Webster, Ph.D. University of Southern California
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon May 12 18:51:50 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4CNpok9018179 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 18:51:50 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id 324C66FEDE4 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 18:51:50 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id LQfLRb0Q4uPp for {microscopy-at-microscopy.com} ; 17, 28 -- Mon, 12 May 2014 18:51:40 -0500 (CDT) 17, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 4CED86FEDD7 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 18:51:40 -0500 (CDT) 17, 28 -- Message-ID: {53715E8B.2040502-at-microscopy.com} 17, 28 -- Date: Mon, 12 May 2014 18:51:39 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Third Party Service for EM 17, 28 -- References: {201405122243.s4CMh1QD014509-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201405122243.s4CMh1QD014509-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201405122243.s4CMh1QD014509-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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We would like to draw your attention that the University of Maryland Baltimore will be hosting the First Annual Current EM Techniques Workshop on June 2 and 3, 2014. The purpose of this annual workshop is to introduce participants to new electron microscopy techniques and instruments via live demonstration and open discussion. The focus of this yearÂ’s workshop is cryo-EM sample preparation and cryo-EM. The workshop will include oral presentations and tips-and-tricks discussion forums during the morning session followed by live instrument demonstration in the afternoon session. Demo instruments will feature high pressure freezer, freeze substitution systems, a cryo-ultramicrotome, a grid plunger, cryo-TEM and cryo-SEM. A dinner event will be co-sponsored by the Chesapeake Microscopy and Microanalysis society (CMMS) on June 2nd providing opportunities for social and scientific exchange among workshop participants and CMMS members.
More information regarding the workshop and registration can be found on our website: http://www.dental.umaryland.edu/Core-imaging/2010web/events/CurrentEMWorkshop.html .
The registration will be open until May 30th.
For other inquiries send email to: UMBCurrentEMWorkshop-at-umaryland.edu
We look forward to see you in Baltimore.
Core Imaging Facility University of Maryland, Baltimore
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon May 12 18:54:46 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4CNskZp019587 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 18:54:46 -0500 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 98D306FEDF7 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 18:54:46 -0500 (CDT) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id GVqHl7p+OGpF for {microscopy-at-microscopy.com} ; 19, 28 -- Mon, 12 May 2014 18:54:41 -0500 (CDT) 19, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 37B806FEDEA 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 18:54:41 -0500 (CDT) 19, 28 -- Message-ID: {53715F40.9040805-at-microscopy.com} 19, 28 -- Date: Mon, 12 May 2014 18:54:40 -0500 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:University of Maryland Baltimore workshop announcement 19, 28 -- References: {201405122304.s4CN4kgN016074-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201405122304.s4CN4kgN016074-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201405122304.s4CN4kgN016074-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 19, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From discounted-medications5-at-vripack.com Mon May 12 21:23:37 2014 Return-Path: {discounted-medications5-at-vripack.com} Received: from mail2.vripack.com (230.18.150.178.triolan.net [178.150.18.230] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4D2NXPI025459 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 12 May 2014 21:23:34 -0500 Message-Id: {201405130223.s4D2NXPI025459-at-ns.microscopy.com}
Dear Paul,
There are not just worlds, but Universes of difference between service from the third-party providers (some of whom are very good and some are not so much), and "cost reduction offers" from the middlemen trying to make a buck by inserting themselves between the end-users and OEM (or third-party) service providers.
Be very careful and read the finest print on whatever service offering you are considering...
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 5/12/2014 7:54 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: pwebster-at-usc.edu () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both pwebster-at-usc.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: pwebster-at-usc.edu } Name: Paul Webster } } Organization: University of Southern California } } Title-Subject: [Filtered] Third Party Service for EM } } Message: Dear All, } } I know this subject comes up often, and I have the same feelings about the subject that are often } expressed on this server. However I can't find the material in the archives. My apologies in advance } for this question. } } So, here goes: Does anyone have any experiences with service being provided through third party } companies such as the Remi Group. } } Past replies have highlighted the lack of specific support for electron microscopes, the difficulty } in getting priority service, the need to pay for parts etc. } } Do these concerns still apply or have the third party providers improved their service? Comments } from anyone who is or has been covered by such a company will be welcome, however,replies from } anyone with an opinion are encouraged (positive and negative). We are preparing our reply to our } bean counters. } } Best regards, } } Paul Webster, Ph.D. } University of Southern California } } Login Host: 68.181.169.204 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
==============================Original Headers============================== 6, 41 -- From vray-at-partbeamsystech.com Mon May 12 21:46:17 2014 6, 41 -- Received: from nm2-vm1.bullet.mail.bf1.yahoo.com (nm2-vm1.bullet.mail.bf1.yahoo.com [98.139.213.158]) 6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4D2kGmF027331 6, 41 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2014 21:46:17 -0500 6, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1399949176; bh=p3zF4phtSywVvWio5rU59Pc+Z0WfRVDt7hMQQbsf+rc=; h=Received:Received:Received:X-Yahoo-Newman-Id:X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:Message-ID:Date:From:Reply-To:User-Agent:MIME-Version:To:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=EXGu9Ft+BEWP452YQ2/79hNKuu9MiuBR6UQ1XL9127ooaxra1jutu9SOi86R6lblfcmyePizOzMfQUoeuiYEK16JDM6veaoCp2IQRAcdEVEW6vsCauAoQL3DquPh7iz5934H3QSyEjT/F9P5XD5r18OUF+z7ppTncxZnS5/V8/d7JjlL0gEPBg2h+V1rVStx2CrH00u2U7mGE9O1GFn5MNDmur6pQ6NqWrJhU5IhdKn7T8CVv/6BM6zF8uPSFAf7Wv2SZC3gG5mINYN4G5kyBn0cc7CKGEsnrOO64Ik25uEcPn+0Ekts79pKlJyt8nr2WxX4jEBVoM/nPOg1BDescw== 6, 41 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s2048; d=yahoo.com; 6, 41 -- b=o6oOamUAc8SjsuK0H1BBazJwOhnEIaBd7EgpkjaQKbnn7jelenFu7CPvTOL+sO4fvVXbd5GP/ehOdD7ZPetGSYralI7IJn039V96JNiLRKgL0aFrlGKElhl2GC6F5FJH9BjjlIofdVgPsxtnWrIluunUiX50uTY3XGTA5h+JuFjbloXG8KSG2ElnmUiEkJP8C7LR4HUUI6VsxqtK0ik0NMNYl6GI9x6VxJnn7lJz+il9zHd10f4/LM4PlF8UulePBgf3x7RbzbHJubxnnD6gdVuMdUzi5dwNOzZ8uJInn1/yCVrisUvLxJT1CLJPG8iDDI8rYZvBNiATD6a/BWSltA==; 6, 41 -- Received: from [66.196.81.172] by nm2.bullet.mail.bf1.yahoo.com with NNFMP; 13 May 2014 02:46:16 -0000 6, 41 -- Received: from [98.139.211.197] by tm18.bullet.mail.bf1.yahoo.com with NNFMP; 13 May 2014 02:46:16 -0000 6, 41 -- Received: from [127.0.0.1] by smtp206.mail.bf1.yahoo.com with NNFMP; 13 May 2014 02:46:16 -0000 6, 41 -- X-Yahoo-Newman-Id: 278941.66194.bm-at-smtp206.mail.bf1.yahoo.com 6, 41 -- X-Yahoo-Newman-Property: ymail-3 6, 41 -- X-YMail-OSG: wgsDgJYVM1klFY3I0EFDUr2sfUMo7SW3OICNXjsed_okLRX 6, 41 -- bRYgZQPWioBNwxb_xRN8E.NHFxGxb_va06wsIDk5eqvzMD_0MSf5Uvm_uxSR 6, 41 -- uS4ZlWgGp79RP5iVSQ1HB301PHU.ajMixXNy60JkD84RFswS91FnV4FLf5Vm 6, 41 -- G0mmIq7CfTX99MrZREhoOPOzSAefZLTMLGPZZ8ogAHjnuHE9C6yNn9zPp7a1 6, 41 -- 4kUEoxZ8S6.rSEQFZiTDOdazsOA58KOKSgtSAgM_5ykOTfTAcn9N9uK_0lTr 6, 41 -- TJj8ocXr5hbkkHbOpuhYpO5gc4brh8Hm8gy0OmMYYLSH9Z0PBaL7NgvBl6hL 6, 41 -- 7AjgQsdWE5Glmu527h9zk7s3jMG7XfQuE.W4hmIZzI0OIR.aRy6RKYdHevms 6, 41 -- m8_ey71DCh1YEEpdQ_ph1PMXBIZpHf_jqNXfI9HguQb_9W4nx0V8nDzBpoB4 6, 41 -- JmDIYSL.Y9EozY6kKySf5WM.9XQAknHyYY_CEXvySjSQ6fzLrt.c1TRtOSI. 6, 41 -- c0HbQo50MT4KthRoagdYa_QM4hhl6ELHnonZeaHOV1Btbxw7qy_266CzhmiS 6, 41 -- 9F73gjp3JZe_nGUC5q9LpTpra9KUtjOTYRo7DIpxTSe8s1aSrxK64ppyCTzU 6, 41 -- V8sBc62b_bZ8pY9hmEe22iEyUsUq0.PlxI63lRCxSwpFuTOx_3wUldtwJsix 6, 41 -- rsR8ZDEgS6.WY875egOwwWwirYoBoiQOg5UixbFnC2i7GpJCIpRo.reQPTHs 6, 41 -- zbUqH 6, 41 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 41 -- X-Rocket-Received: from [172.16.11.16] (vray-at-68.167.50.54 with plain [98.138.105.21]) 6, 41 -- by smtp206.mail.bf1.yahoo.com with SMTP; 12 May 2014 19:46:16 -0700 PDT 6, 41 -- Message-ID: {53718773.5060204-at-partbeamsystech.com} 6, 41 -- Date: Mon, 12 May 2014 22:46:11 -0400 6, 41 -- From: Valery Ray {vray-at-partbeamsystech.com} 6, 41 -- Reply-To: vray-at-partbeamsystech.com 6, 41 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 6, 41 -- MIME-Version: 1.0 6, 41 -- To: microscopy-at-microscopy.com, pwebster-at-usc.edu 6, 41 -- Subject: Re: [Microscopy] viaWWW:Third Party Service for EM 6, 41 -- References: {201405122354.s4CNsYbb019529-at-ns.microscopy.com} 6, 41 -- In-Reply-To: {201405122354.s4CNsYbb019529-at-ns.microscopy.com} 6, 41 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From canadian-meds11-at-airpurifyingsystems.com Tue May 13 04:23:45 2014 Return-Path: {canadian-meds11-at-airpurifyingsystems.com} Received: from smtp2.airpurifyingsystems.com ([81.162.102.245]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4D9NgZY024589 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 13 May 2014 04:23:43 -0500 Message-Id: {201405130923.s4D9NgZY024589-at-ns.microscopy.com}
First, I want to thank the members of this list for all the help keeping our donated SEM running for the past year. The advice and connections I've made have been extremely valuable.
Now I'm asking a different kind of question. Some background:
Pumping Station: One is a non-profit "hackerspace" or "makerspace" located in Chicago. We are an organization that provides members with access to all sorts of tools (woodworking, machine shop, electronics, welding, CNC, 3D printing, laser cutting, home brewing and cooking, sewing and machine knitting, etc.) but more than that, we have built a community of around 270 makers working on projects, offering classes, and offering plenty of informal mentoring and support for each other's projects.
So, last year, we received a donation of a Leica S430. We believe we are the only hackerspace with a working SEM. I've been building up infrastructure for a microscopy lab around it. The scope works reliably (even if the vacuum is not as good as I'd like) with a repairable BSD and a working EDX, and we have a working sputter coater, critical point dryer, and ultrasonic cleaner. I'm finishing up a project to have bulk liquid nitrogen available, and it looks like I might have a source for a binocular inspection microscope for sample prep.
Now I'm focusing on the "people" side of things, and what to do with all of this. We're in an unusual situation. Universities and labs have reasons to own a SEM. High schools with donated SEMs have curricula / after school programs. While our organization is educational, it isn't formal in the sense of a school, and a SEM is really an extra or bonus.
I've put together a training program which is popular. People like to learn new tools. I've got more than 20 people authorized to use it on their own. Yet there has been very little use outside of the training; often people don't touch it afterwards. (In fairness, this is true of other tools too, e.g. welding, machining....)
Part of it may be that I have not yet started offering sample prep classes / authorizations, although I've made it clear that I'm happy to prepare samples for people and load them in the chamber. So far only two people have taken me up on that. Admittedly, sample prep is an area I know little about, especially biological sample prep. (Did I mention that I'm pretty much self taught in all this?) The CPD was an impulse buy on eBay, before I realized we need to do glutaraldehyde fixing first which needs a fume hood to do safely, so I'm not sure how to handle wet biological samples. I'm left with a lot of questions. Like whether PLA or ABS plastics outgas at SEM vacuum levels, and whether they could be sputter coated and wouldn't melt under the beam. (We do a lot of 3D printing, and it would be interesting to compare these.) Or how to mount specimens with small particulates. (I made a trip to the Chernobyl exclusion zone, and I'd like to do EDX analysis on samples collected.)
So I'm left puzzled. I think it is unprecedented to have an SEM available 24/7, free of charge with free training, to the general public in the middle of a major city, yet nobody seems to be taking advantage of it. (The general public would have to become members to use it, but membership is open to anyone 18 and older for $40 a month, and that includes access to all the other tools.)
I'm not sure if people are intimidated by it, or if they have a hard time thinking of applications for it. I've considered having open "SEM Salons" where we would have a sample or project to work on as a group. But by and large, I'm looking for ideas of stuff to do, methods for sample prep, ways to do outreach to get people involved, etc.
I'm also looking for groups to partner with. High schools are problematic at this time because we are still trying to nail down liability issues around minors. Collaboration with for-profit groups would be fine too, provided they did participate and give back to our community.
And, of course, if anyone on this list is local to Chicago, we are looking for members to get involved with the work we are doing.
Hackerspaces are a relatively new concept, and while we may be the only one now with a working SEM, I think that might change. I know a hackerspace in the Washington DC area is repairing a Cambridge Stereoscan 200. So I'm very much interested in best practices surrounding open science and public SEM access.
Regards, Ryan Pierce
==============================Original Headers============================== 16, 17 -- From rdpierce-at-pobox.com Tue May 13 07:51:02 2014 16, 17 -- Received: from elna.mackenziegems.com (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4DCp0EP029538 16, 17 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2014 07:51:01 -0500 16, 17 -- Received: from [127.0.0.1] (localhost [127.0.0.1]) 16, 17 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id 1496A6E03B4 16, 17 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2014 07:51:00 -0500 (CDT) 16, 17 -- Message-ID: {53721533.1090405-at-pobox.com} 16, 17 -- Date: Tue, 13 May 2014 07:50:59 -0500 16, 17 -- From: Ryan Pierce {rdpierce-at-pobox.com} 16, 17 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:24.0) Gecko/20100101 Thunderbird/24.3.0 16, 17 -- MIME-Version: 1.0 16, 17 -- To: microscopy-at-microscopy.com 16, 17 -- Subject: SEM: Suggestions for hackerspace electron microscopy in Chicago 16, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 17 -- Content-Transfer-Encoding: 8bit 16, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4DCp0EP029538 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both valerie.lecomte-at-usherbrooke.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
I want to do quantitative analysis on minerals. I am currently having some problems with calibrations for arsenopyrite and I would like if someone can share with me his own experience on that kind of analysis.
Is there anyone use to do quantitative analysis on minerals? Please contact me and I will reply to your email with all the details.
Thanks for your help! Valerie
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue May 13 08:53:35 2014 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4DDrZjh016935 15, 28 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2014 08:53:35 -0500 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id D75A26FF47C 15, 28 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2014 08:53:35 -0500 (CDT) 15, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id KG40TCoqRMbb for {microscopy-at-microscopy.com} ; 15, 28 -- Tue, 13 May 2014 08:53:30 -0500 (CDT) 15, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 95F3C6FF46A 15, 28 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2014 08:53:30 -0500 (CDT) 15, 28 -- Message-ID: {537223DA.9060405-at-microscopy.com} 15, 28 -- Date: Tue, 13 May 2014 08:53:30 -0500 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:minerals analysis 15, 28 -- References: {201405131248.s4DCmAxW029507-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201405131248.s4DCmAxW029507-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201405131248.s4DCmAxW029507-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Friends, Can you please advise me where I can find a concise (and scientific accurate) definition for transmission electron microscopy? Also for scanning electron microscopy? Zhaoxia Zhou Dr Z Zhou Loughborough University Leicestershire UK
==============================Original Headers============================== 2, 31 -- From Z.Zhou-at-lboro.ac.uk Tue May 13 10:40:23 2014 2, 31 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 2, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4DFeNrO004895 2, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 13 May 2014 10:40:23 -0500 2, 31 -- Received: from [158.125.167.10] (helo=ITSCASH-3.lunet.lboro.ac.uk) 2, 31 -- by mta-1.lboro.ac.uk with esmtps (TLSv1:AES128-SHA:128) 2, 31 -- (Exim 4.80) 2, 31 -- id 1WkEoU-0007xs-Rl 2, 31 -- for Microscopy-at-microscopy.com; Tue, 13 May 2014 16:40:18 +0100 2, 31 -- Received: from ITSMBX-4.lunet.lboro.ac.uk ([169.254.1.190]) by 2, 31 -- ITSCASH-3.lunet.lboro.ac.uk ([158.125.167.10]) with mapi id 14.03.0174.001; 2, 31 -- Tue, 13 May 2014 16:40:18 +0100 2, 31 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} 2, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 31 -- Subject: definition for TEM and SEM 2, 31 -- Thread-Topic: definition for TEM and SEM 2, 31 -- Thread-Index: Ac9uwU4pk4Y2gG69QMOt8Be3W78Y8g== 2, 31 -- Date: Tue, 13 May 2014 15:40:17 +0000 2, 31 -- Message-ID: {FE28B2DD3D5CBB44BCF7D66B3AF8DFCC31631609-at-ITSMBX-4.lunet.lboro.ac.uk} 2, 31 -- Accept-Language: en-GB, en-US 2, 31 -- Content-Language: en-US 2, 31 -- X-MS-Has-Attach: 2, 31 -- X-MS-TNEF-Correlator: 2, 31 -- x-originating-ip: [158.125.69.240] 2, 31 -- Content-Type: text/plain; charset="utf-8" 2, 31 -- MIME-Version: 1.0 2, 31 -- X-Scan-Signature: a29bf63011d59dfae58451b664a4607b 2, 31 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 2, 31 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Tue, 13 May 2014 16:40:22 +0100 2, 31 -- Content-Transfer-Encoding: 8bit 2, 31 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s4DFeNrO004895 ==============================End of - Headers==============================
From meds-popular8-at-rowdyism.com Tue May 13 13:13:22 2014 Return-Path: {meds-popular8-at-rowdyism.com} Received: from smtp1.rowdyism.com (149.32.150.178.triolan.net [178.150.32.149] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4DIDIwL028380 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 13 May 2014 13:13:19 -0500 Message-Id: {201405131813.s4DIDIwL028380-at-ns.microscopy.com}
We have an old Polaron evaporator - I don't know how old as the labeling is practically non-existent. Anyway, we seem to have lost the solenoid that operates the baffle valve underneath the bell jar. Apparently it has been known to stick and it reportedly took some coaxing to make sure it opened. Anyway, it appears to have been stuck yesterday and it is now rather crispy. Perhaps it is still functional, but it certainly got very hot.
Just in case it is fried, does anyone happen to have one of these laying around that they are not using that we might be able to get parts?
Warren Straszheim Ames Laboratory Ames, IA
==============================Original Headers============================== 3, 35 -- From wesaia-at-iastate.edu Tue May 13 13:56:00 2014 3, 35 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2on0108.outbound.protection.outlook.com [207.46.100.108]) 3, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4DItx7j031726 3, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 13 May 2014 13:55:59 -0500 3, 35 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 3, 35 -- BLUPR04MB739.namprd04.prod.outlook.com (10.141.207.21) with Microsoft SMTP 3, 35 -- Server (TLS) id 15.0.944.11; Tue, 13 May 2014 18:55:58 +0000 3, 35 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 3, 35 -- BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) with Microsoft SMTP 3, 35 -- Server (TLS) id 15.0.944.11; Tue, 13 May 2014 18:55:56 +0000 3, 35 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.75]) by 3, 35 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.75]) with mapi id 3, 35 -- 15.00.0944.000; Tue, 13 May 2014 18:55:56 +0000 3, 35 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 3, 35 -- To: MSA {Microscopy-at-microscopy.com} 3, 35 -- Subject: Polaron evaporator woes 3, 35 -- Thread-Topic: Polaron evaporator woes 3, 35 -- Thread-Index: AQHPbtsydu5m8DvcuUaCXeP86czz/A== 3, 35 -- Date: Tue, 13 May 2014 18:55:55 +0000 3, 35 -- Message-ID: {1400007340602.19052-at-iastate.edu} 3, 35 -- Accept-Language: en-US 3, 35 -- Content-Language: en-US 3, 35 -- X-MS-Has-Attach: 3, 35 -- X-MS-TNEF-Correlator: 3, 35 -- x-originating-ip: [129.186.227.3] 3, 35 -- x-forefront-prvs: 0210479ED8 3, 35 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019001)(6009001)(428001)(189002)(199002)(52314003)(87936001)(46102001)(101416001)(80022001)(77982001)(81342001)(86362001)(85852003)(75432001)(92726001)(79102001)(66066001)(2656002)(20776003)(64706001)(92566001)(99396002)(83072002)(54356999)(4396001)(21056001)(50986999)(31966008)(76482001)(81542001)(74662001)(74502001)(83322001)(36756003);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR04MB546;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 3, 35 -- received-spf: None (: iastate.edu does not designate permitted sender hosts) 3, 35 -- authentication-results: spf=none (sender IP is ) 3, 35 -- smtp.mailfrom=wesaia-at-iastate.edu; 3, 35 -- Content-Type: text/plain; charset="iso-8859-1" 3, 35 -- MIME-Version: 1.0 3, 35 -- X-OriginatorOrg: iastate.edu 3, 35 -- Content-Transfer-Encoding: 8bit 3, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4DItx7j031726 ==============================End of - Headers==============================
I suggest that with a working SEM and EDS you contact some mineralogical clubs in the area. At least I would be very interested in running unknown samples, too bad I live in Sweden.
But, on the other hand I have a TEM and a SEM in my private hackerspace. :-D
I'm partly attached to the local hackerspace here in Umeĺ, I know several of the members, but so far I have more toys and bigger space where I store my "junk" so even if I wanted to I can't donate anything to them, they don't have the space for it. I started my journey into the EM world since I collect minerals and there are always strange things you can't identify with simple means. So I got a donated TEM, restored it (it took a year) and when I got it running I promptly melted some asbestos fibres in the electron beam... there's quite a punch if you focus it good enough! My plan was to set up a lab to run my own samples and others, not quite there yet. When I've talked about it with fellow collectors I got quite a lot of interest. Therefore I suggest you contact mineral societies in the area, I'm sure there is large interest among them.
I'm in a middle of a move from one storage to a bigger one, and when that is finished I will bring the microscopes up again. The only thing I'm lacking is a working EDS. I have a system but the computer is from 1974 and uses core memory, that in it self will be quite a project to get running. :-D What I have is a JEOL JEM 100CX TEM with ASID, a JSM-25 SEM, a JEOL carbon evaporator, and a water chiller. It could be a fun project on rainy summer days to put everything in working order again after the move.
Regards, Göran
rdpierce-at-pobox.com skrev 2014-05-13 14:51: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } First, I want to thank the members of this list for all the help keeping our donated SEM running for the past year. The advice and connections I've made have been extremely valuable. } } Now I'm asking a different kind of question. Some background: } } Pumping Station: One is a non-profit "hackerspace" or "makerspace" located in Chicago. We are an organization that provides members with access to all sorts of tools (woodworking, machine shop, electronics, welding, CNC, 3D printing, laser cutting, home brewing and cooking, sewing and machine knitting, etc.) but more than that, we have built a community of around 270 makers working on projects, offering classes, and offering plenty of informal mentoring and support for each other's projects. } } So, last year, we received a donation of a Leica S430. We believe we are the only hackerspace with a working SEM. I've been building up infrastructure for a microscopy lab around it. The scope works reliably (even if the vacuum is not as good as I'd like) with a repairable BSD and a working EDX, and we have a working sputter coater, critical point dryer, and ultrasonic cleaner. I'm finishing up a project to have bulk liquid nitrogen available, and it looks like I might have a source for a binocular inspection microscope for sample prep. } } Now I'm focusing on the "people" side of things, and what to do with all of this. We're in an unusual situation. Universities and labs have reasons to own a SEM. High schools with donated SEMs have curricula / after school programs. While our organization is educational, it isn't formal in the sense of a school, and a SEM is really an extra or bonus. } } I've put together a training program which is popular. People like to learn new tools. I've got more than 20 people authorized to use it on their own. Yet there has been very little use outside of the training; often people don't touch it afterwards. (In fairness, this is true of other tools too, e.g. welding, machining....) } } Part of it may be that I have not yet started offering sample prep classes / authorizations, although I've made it clear that I'm happy to prepare samples for people and load them in the chamber. So far only two people have taken me up on that. Admittedly, sample prep is an area I know little about, especially biological sample prep. (Did I mention that I'm pretty much self taught in all this?) The CPD was an impulse buy on eBay, before I realized we need to do glutaraldehyde fixing first which needs a fume hood to do safely, so I'm not sure how to handle wet biological samples. I'm left with a lot of questions. Like whether PLA or ABS plastics outgas at SEM vacuum levels, and whether they could be sputter coated and wouldn't melt under the beam. (We do a lot of 3D printing, and it would be interesting to compare these.) Or how to mount specimens with small particulates. (I made a trip to the Chernobyl exclusion zone, and I'd like to do EDX analysis on samples collected.) } } So I'm left puzzled. I think it is unprecedented to have an SEM available 24/7, free of charge with free training, to the general public in the middle of a major city, yet nobody seems to be taking advantage of it. (The general public would have to become members to use it, but membership is open to anyone 18 and older for $40 a month, and that includes access to all the other tools.) } } I'm not sure if people are intimidated by it, or if they have a hard time thinking of applications for it. I've considered having open "SEM Salons" where we would have a sample or project to work on as a group. But by and large, I'm looking for ideas of stuff to do, methods for sample prep, ways to do outreach to get people involved, etc. } } I'm also looking for groups to partner with. High schools are problematic at this time because we are still trying to nail down liability issues around minors. Collaboration with for-profit groups would be fine too, provided they did participate and give back to our community. } } And, of course, if anyone on this list is local to Chicago, we are looking for members to get involved with the work we are doing. } } Hackerspaces are a relatively new concept, and while we may be the only one now with a working SEM, I think that might change. I know a hackerspace in the Washington DC area is repairing a Cambridge Stereoscan 200. So I'm very much interested in best practices surrounding open science and public SEM access. } } Regards, } Ryan Pierce
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Email: sdillon-at-illinois.edu Name: Shen Dillon
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Title-Subject: [Filtered] Postdoc in in-situ nanomechanics
Message: Hello Everyone,
Our group is currently looking for a postdoctoral microscopist to work on in-situ nanomechanical testing of tribofilms and solid-state lubricants. The position would require some travel to visit with collaborators in the UK. If you know anyone who may be interested, please suggest that they contact me. Thanks!
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An Invitation to MSA Student Members Microscopy & Microanalysis 2014-Hartford “Lunch with a Manager”
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Email: kalennon-at-hagerstowncc.edu Name: Kristen Lennon
Organization: Hagerstown Community College
Title-Subject: [Filtered] hand microtome
Message: Hi Fellow Microscopy Enthusiasts,
Do any of you have experience using a hand-held microtome? I have a colleague who is interested in purchasing one to use as part of an introduction to microscopy class for our undergraduates. Hand-held is not something I've ever used, and I'm a bit skeptical. I'd love to get her at least a vibratome, but I'd bet they are out of our current price range.
Any advice?
Thanks once again for your help, Kristen Lennon
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed May 14 18:55:19 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4ENtJdq010063 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 18:55:19 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 993E9700417 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 18:55:19 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id lzWusV9LnDnt for {microscopy-at-microscopy.com} ; 13, 28 -- Wed, 14 May 2014 18:55:15 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id B3AC370040A 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 18:55:15 -0500 (CDT) 13, 28 -- Message-ID: {53740263.4080505-at-microscopy.com} 13, 28 -- Date: Wed, 14 May 2014 18:55:15 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:hand-held microtome 13, 28 -- References: {201405140100.s4E10bGa012217-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201405140100.s4E10bGa012217-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201405140100.s4E10bGa012217-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Donation SEMs often languish, because infrastructure and upkeep are far more work than people realize, plus, they can't be put just anywhere due to vibration issues. Pumps need to be vented. Sample prep isn't easy, and often the necessary equipment for that is not donated along with it.
Protocols using gluteraldehyde and/or osmium tetroxide are especially tricky, because a fume hood is rarely part of the donation. (You might actually consider building something like this at your hackerspace.)
You've gotten much further than Noisebridge in San Francisco, where some effort was expended to restore a tabletop SEM that was donated, but it largely languished. Noisebridge is notoriously disorganized and in decline, however.
You might consider a sliding scale fee for use of the SEM. If you maintain the device at the level you should be, there's some definite expense. SEM aperture plates and column apertures need to be replaced annually, and that's a few hundred dollars each time. Also, oil changes for the vacuum pumps should be done every 6 months, and the special machine oil needed for that is kind of spendy. The vacuum pumps themselves have a finite lifespan, and can be pretty expensive to replace when they've reached the end of their life. And finding replacements for parts for the SEM itself can be expensive if they should break, if you can find them at all.
SEM can be a very good entry into science, not to mention a valuable skill for high school students, if you can deal with the liability issues. There are plenty of nonprofit educational efforts that successfully negotiate this, however. And high schools aren't the be-all-end-all for educational partnerships - consider community colleges. Currently, I'm SEM tech at Ohlone College, which is one of the few community colleges with an SEM and SEM classes. The other being Delta College, which has an entire EM program. There may be a few other examples. What I can tell you can find many people at that level who are interested and would love the opportunity to work on such a device. Consider working with biotech, biology, engineering, and other science classes. It's possible to even offer an SEM class with the hackerspace as an off-site - it might be possible to get some upkeep money for the instrument this way as well.
Keep in touch about this - I'd be interested to know how your hackerspace SEM project is going.
Cheers! Peter G. Werner Microscopy and Physical Science Lab Technician, Ohlone College Lab Technician/Instructional Assistant, Microscopy, Merritt College Acting President, San Francisco Microscopical Society pwerner-at-ohlone.edu (510) 979-7911 (office) (415) 261-7114 (cell)
On 2014-05-13 06:00, rdpierce-at-pobox.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } First, I want to thank the members of this list for all the help } keeping our donated SEM running for the past year. The advice and } connections I've made have been extremely valuable. } } Now I'm asking a different kind of question. Some background: } } Pumping Station: One is a non-profit "hackerspace" or "makerspace" } located in Chicago. We are an organization that provides members with } access to all sorts of tools (woodworking, machine shop, electronics, } welding, CNC, 3D printing, laser cutting, home brewing and cooking, } sewing and machine knitting, etc.) but more than that, we have built a } community of around 270 makers working on projects, offering classes, } and offering plenty of informal mentoring and support for each other's } projects. } } So, last year, we received a donation of a Leica S430. We believe we } are the only hackerspace with a working SEM. I've been building up } infrastructure for a microscopy lab around it. The scope works } reliably (even if the vacuum is not as good as I'd like) with a } repairable BSD and a working EDX, and we have a working sputter } coater, critical point dryer, and ultrasonic cleaner. I'm finishing up } a project to have bulk liquid nitrogen available, and it looks like I } might have a source for a binocular inspection microscope for sample } prep. } } Now I'm focusing on the "people" side of things, and what to do with } all of this. We're in an unusual situation. Universities and labs have } reasons to own a SEM. High schools with donated SEMs have curricula / } after school programs. While our organization is educational, it isn't } formal in the sense of a school, and a SEM is really an extra or } bonus. } } I've put together a training program which is popular. People like to } learn new tools. I've got more than 20 people authorized to use it on } their own. Yet there has been very little use outside of the training; } often people don't touch it afterwards. (In fairness, this is true of } other tools too, e.g. welding, machining....) } } Part of it may be that I have not yet started offering sample prep } classes / authorizations, although I've made it clear that I'm happy } to prepare samples for people and load them in the chamber. So far } only two people have taken me up on that. Admittedly, sample prep is } an area I know little about, especially biological sample prep. (Did I } mention that I'm pretty much self taught in all this?) The CPD was an } impulse buy on eBay, before I realized we need to do glutaraldehyde } fixing first which needs a fume hood to do safely, so I'm not sure how } to handle wet biological samples. I'm left with a lot of questions. } Like whether PLA or ABS plastics outgas at SEM vacuum levels, and } whether they could be sputter coated and wouldn't melt under the beam. } (We do a lot of 3D printing, and it would be interesting to compare } these.) Or how to mount specimens with small particulates. (I made a } trip to the Chernobyl exclusion zone, and I'd like to do EDX analysis } on samples collected.) } } So I'm left puzzled. I think it is unprecedented to have an SEM } available 24/7, free of charge with free training, to the general } public in the middle of a major city, yet nobody seems to be taking } advantage of it. (The general public would have to become members to } use it, but membership is open to anyone 18 and older for $40 a month, } and that includes access to all the other tools.) } } I'm not sure if people are intimidated by it, or if they have a hard } time thinking of applications for it. I've considered having open "SEM } Salons" where we would have a sample or project to work on as a group. } But by and large, I'm looking for ideas of stuff to do, methods for } sample prep, ways to do outreach to get people involved, etc. } } I'm also looking for groups to partner with. High schools are } problematic at this time because we are still trying to nail down } liability issues around minors. Collaboration with for-profit groups } would be fine too, provided they did participate and give back to our } community. } } And, of course, if anyone on this list is local to Chicago, we are } looking for members to get involved with the work we are doing. } } Hackerspaces are a relatively new concept, and while we may be the } only one now with a working SEM, I think that might change. I know a } hackerspace in the Washington DC area is repairing a Cambridge } Stereoscan 200. So I'm very much interested in best practices } surrounding open science and public SEM access. } } Regards, } Ryan Pierce } } } } } ==============================Original } Headers============================== } 16, 17 -- From rdpierce-at-pobox.com Tue May 13 07:51:02 2014 } 16, 17 -- Received: from elna.mackenziegems.com } (dsl253-036-183.chi1.dsl.speakeasy.net [66.253.36.183]) } 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s4DCp0EP029538 } 16, 17 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2014 07:51:01 } -0500 } 16, 17 -- Received: from [127.0.0.1] (localhost [127.0.0.1]) } 16, 17 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id } 1496A6E03B4 } 16, 17 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2014 07:51:00 } -0500 (CDT) } 16, 17 -- Message-ID: {53721533.1090405-at-pobox.com} } 16, 17 -- Date: Tue, 13 May 2014 07:50:59 -0500 } 16, 17 -- From: Ryan Pierce {rdpierce-at-pobox.com} } 16, 17 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:24.0) } Gecko/20100101 Thunderbird/24.3.0 } 16, 17 -- MIME-Version: 1.0 } 16, 17 -- To: microscopy-at-microscopy.com } 16, 17 -- Subject: SEM: Suggestions for hackerspace electron } microscopy in Chicago } 16, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 16, 17 -- Content-Transfer-Encoding: 8bit } 16, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s4DCp0EP029538 } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 27 -- From germpore-at-sonic.net Wed May 14 19:15:46 2014 9, 27 -- Received: from c.mail.sonic.net (c.mail.sonic.net [64.142.111.80]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4F0FkW0032253 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 19:15:46 -0500 9, 27 -- Received: from webmail.sonic.net (webmail.b.apps.sonic.net [64.142.122.9]) 9, 27 -- (authenticated bits=0) 9, 27 -- by c.mail.sonic.net (8.14.4/8.14.4) with ESMTP id s4F0Fh6o032300; 9, 27 -- Wed, 14 May 2014 17:15:43 -0700 9, 27 -- Received: from fw-a.ohlone.edu ([207.62.190.18]) 9, 27 -- by webmail.sonic.net 9, 27 -- with HTTP (HTTP/1.1 POST); Wed, 14 May 2014 17:15:43 -0700 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; charset=UTF-8; 9, 27 -- format=flowed 9, 27 -- Content-Transfer-Encoding: 7bit 9, 27 -- Date: Wed, 14 May 2014 17:15:43 -0700 9, 27 -- From: Peter Werner {germpore-at-sonic.net} 9, 27 -- To: rdpierce-at-pobox.com, microscopy-at-microscopy.com 9, 27 -- Subject: Re: [Microscopy] SEM: Suggestions for hackerspace electron 9, 27 -- microscopy in Chicago 9, 27 -- In-Reply-To: {201405131300.s4DD0OQj009386-at-ns.microscopy.com} 9, 27 -- References: {201405131300.s4DD0OQj009386-at-ns.microscopy.com} 9, 27 -- Message-ID: {4bbb181d5241966dc3ce667fa7bfd17e-at-sonic.net} 9, 27 -- User-Agent: Roundcube Webmail/0.9.5 9, 27 -- X-Sonic-Auth: SEp6yTzEQI7V6xw1xV134E3cpQiWS8WPs7lFf/5occfIGOnbwpG1KWQiQjoCXqT2zANLHy5XGSQIwYnwcvxqpzjSdupMN8fvzsLpXqnWlZY= 9, 27 -- X-Sender: (germpore-at-sonic.net) 9, 27 -- X-Sonic-ID: C;HmcXDsbb4xGiXLp3oNQ8UQ== M;3joaDsbb4xGiXLp3oNQ8UQ== ==============================End of - Headers==============================
For undergrad classes, we've always just used double-edged razor blades, snapped in half. Students can either hold the tissue down with their finger and slice across (like slicing an onion), or put the tissue between pieces of potato or carrot to support it while cutting.
Students learn this easily, just need to start them on some big, easy to cut tissue, e.g. Coleus stem or similar. Our 2nd year students learned all their plant anatomy by doing a project sectioning differen parts of a native seedling, some of these were quite woody! Reason for selecting these plants is that there are no diagrams to copy from books, so they have to look at their own tissue. (Cynical from experience.)
We have a couple of hand microtomes, they don't get used much. You need a good old-fashioned large (beard) shaving blade to get good sections from this, which is more dangerous than razor blades in my opinion.
A simple one is described here: http://www.microscope-microscope.org/activities/school/microtome.htm
But see http://prometheuswiki.publish.csiro.au/tiki-index.php?page=Making+hand+sect ions+without+support+material for hand sectioning without support material, and in the grey box on that page are some alternatives using various support materials.
We still do most of our sectioning by hand...
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 15/05/14 10:17 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
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==============================Original Headers============================== 14, 47 -- From prvs=2055cbd7d=Rosemary.White-at-csiro.au Wed May 14 19:55:57 2014 14, 47 -- Received: from act-MTAout3.csiro.au (act-MTAout3.csiro.au [150.229.7.39]) 14, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4F0ttxU025598 14, 47 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 19:55:56 -0500 14, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 14, 47 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; 14, 47 -- t=1400115357; x=1431651357; 14, 47 -- h=from:to:cc:subject:date:message-id:in-reply-to: 14, 47 -- content-id:content-transfer-encoding:mime-version; 14, 47 -- bh=mgHADN34yIMke1kEzB4Fh3rFIL5S+hIp+FeCnWTkp+o=; 14, 47 -- b=j3aULBWf8cpJZJJ/a0LlqX9wK3goNtwQoq+pVByYJwLp00m533Rk6PSo 14, 47 -- Gz282j8G/tsB6V7kXgtO3DKKc1IR6k/wD4KP6z+TD9xgxgm5fnb0pHChr 14, 47 -- NfFQ+6YFDwsdw9H; 14, 47 -- X-SBRS: 5.1 14, 47 -- X-IronPort-Anti-Spam-Filtered: true 14, 47 -- X-IronPort-Anti-Spam-Result: AnsMADMQdFOYU/cYl2dsb2JhbAA/Gg6CV3BYvjyFaoEAUQGBIxYOAQEBAQEIFgc8giUBBjoUKxIBCBEDAQINCgEHQQEUCQgCBAoEBRQJBIggAQw20HUXjEQRgRcRAR0zBwYMAYIhD0SBOQSZUYE9lE1AbQGBAgcXIg 14, 47 -- X-IPAS-Result: AnsMADMQdFOYU/cYl2dsb2JhbAA/Gg6CV3BYvjyFaoEAUQGBIxYOAQEBAQEIFgc8giUBBjoUKxIBCBEDAQINCgEHQQEUCQgCBAoEBRQJBIggAQw20HUXjEQRgRcRAR0zBwYMAYIhD0SBOQSZUYE9lE1AbQGBAgcXIg 14, 47 -- X-IronPort-AV: E=Sophos;i="4.97,1055,1389704400"; 14, 47 -- d="scan'208";a="117547613" 14, 47 -- Received: from exhtca04-cdc.nexus.csiro.au ([152.83.247.24]) 14, 47 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES128-SHA; 15 May 2014 10:55:54 +1000 14, 47 -- Received: from EXHTCA01-CDC.nexus.csiro.au (2405:b000:601:13::247:21) by 14, 47 -- exhtca04-cdc.nexus.csiro.au (2405:b000:601:13::247:24) with Microsoft SMTP 14, 47 -- Server (TLS) id 14.3.158.1; Thu, 15 May 2014 10:55:54 +1000 14, 47 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80::9de3:d7af:4ed1:b7b6]) by 14, 47 -- exhtca01-cdc.nexus.csiro.au ([fe80::296a:b134:b85b:89de%13]) with mapi id 14, 47 -- 14.03.0158.001; Thu, 15 May 2014 10:55:54 +1000 14, 47 -- From: {Rosemary.White-at-csiro.au} 14, 47 -- To: {kalennon-at-hagerstowncc.edu} 14, 47 -- CC: {microscopy-at-microscopy.com} 14, 47 -- Subject: Re: [Microscopy] viaWWW:hand-held microtome 14, 47 -- Thread-Topic: [Microscopy] viaWWW:hand-held microtome 14, 47 -- Thread-Index: AQHPb9NSJyFARW/L1keE7RdjnH26k5tA0S8A 14, 47 -- Date: Thu, 15 May 2014 00:55:53 +0000 14, 47 -- Message-ID: {CF9A4AC2.151EBC%rosemary.white-at-csiro.au} 14, 47 -- In-Reply-To: {201405150017.s4F0H7MR001899-at-ns.microscopy.com} 14, 47 -- Accept-Language: en-NZ, en-US, en-AU 14, 47 -- Content-Language: en-US 14, 47 -- X-MS-Has-Attach: 14, 47 -- X-MS-TNEF-Correlator: 14, 47 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 14, 47 -- x-originating-ip: [152.83.195.117] 14, 47 -- Content-Type: text/plain; charset="us-ascii" 14, 47 -- Content-ID: {85EA60F2E4D2884CA3E1D70B11EEC36B-at-csiro.au} 14, 47 -- MIME-Version: 1.0 14, 47 -- Content-Transfer-Encoding: 8bit 14, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4F0ttxU025598 ==============================End of - Headers==============================
From popular_medications9-at-margotcamille.com Thu May 15 04:36:46 2014 Return-Path: {popular_medications9-at-margotcamille.com} Received: from mail1.margotcamille.com (user-5-173-65-13.play-internet.pl [5.173.65.13]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4F9ackJ026050 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 15 May 2014 04:36:39 -0500 Message-Id: {201405150936.s4F9ackJ026050-at-ns.microscopy.com}
Let me recommend single edge razors like GEM. They are sharp, have a safe back to press against, and are affordable. I thin and cross section a lot plastic and rubber components and they work fine.
Keep your fingers attached............ Frank
-----Original Message----- X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Sent: Wednesday, May 14, 2014 9:16 PM To: Frank Karl
For undergrad classes, we've always just used double-edged razor blades, snapped in half. Students can either hold the tissue down with their finger and slice across (like slicing an onion), or put the tissue between pieces of potato or carrot to support it while cutting.
Students learn this easily, just need to start them on some big, easy to cut tissue, e.g. Coleus stem or similar. Our 2nd year students learned all their plant anatomy by doing a project sectioning differen parts of a native seedling, some of these were quite woody! Reason for selecting these plants is that there are no diagrams to copy from books, so they have to look at their own tissue. (Cynical from experience.)
We have a couple of hand microtomes, they don't get used much. You need a good old-fashioned large (beard) shaving blade to get good sections from this, which is more dangerous than razor blades in my opinion.
A simple one is described here: http://www.microscope-microscope.org/activities/school/microtome.htm
But see http://prometheuswiki.publish.csiro.au/tiki-index.php?page=Making+hand+sect ions+without+support+material for hand sectioning without support material, and in the grey box on that page are some alternatives using various support materials.
We still do most of our sectioning by hand...
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 15/05/14 10:17 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
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==============================Original Headers============================== 14, 47 -- From prvs=2055cbd7d=Rosemary.White-at-csiro.au Wed May 14 19:55:57 2014 14, 47 -- Received: from act-MTAout3.csiro.au (act-MTAout3.csiro.au [150.229.7.39]) 14, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4F0ttxU025598 14, 47 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 19:55:56 -0500 14, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 14, 47 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; 14, 47 -- t=1400115357; x=1431651357; 14, 47 -- h=from:to:cc:subject:date:message-id:in-reply-to: 14, 47 -- content-id:content-transfer-encoding:mime-version; 14, 47 -- bh=mgHADN34yIMke1kEzB4Fh3rFIL5S+hIp+FeCnWTkp+o=; 14, 47 -- b=j3aULBWf8cpJZJJ/a0LlqX9wK3goNtwQoq+pVByYJwLp00m533Rk6PSo 14, 47 -- Gz282j8G/tsB6V7kXgtO3DKKc1IR6k/wD4KP6z+TD9xgxgm5fnb0pHChr 14, 47 -- NfFQ+6YFDwsdw9H; 14, 47 -- X-SBRS: 5.1 14, 47 -- X-IronPort-Anti-Spam-Filtered: true 14, 47 -- X-IronPort-Anti-Spam-Result: AnsMADMQdFOYU/cYl2dsb2JhbAA/Gg6CV3BYvjyFaoEAUQGBIxYOAQEBAQEIFgc8giUBBjoUKxIBCBEDAQINCgEHQQEUCQgCBAoEBRQJBIggAQw20HUXjEQRgRcRAR0zBwYMAYIhD0SBOQSZUYE9lE1AbQGBAgcXIg 14, 47 -- X-IPAS-Result: AnsMADMQdFOYU/cYl2dsb2JhbAA/Gg6CV3BYvjyFaoEAUQGBIxYOAQEBAQEIFgc8giUBBjoUKxIBCBEDAQINCgEHQQEUCQgCBAoEBRQJBIggAQw20HUXjEQRgRcRAR0zBwYMAYIhD0SBOQSZUYE9lE1AbQGBAgcXIg 14, 47 -- X-IronPort-AV: E=Sophos;i="4.97,1055,1389704400"; 14, 47 -- d="scan'208";a="117547613" 14, 47 -- Received: from exhtca04-cdc.nexus.csiro.au ([152.83.247.24]) 14, 47 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES128-SHA; 15 May 2014 10:55:54 +1000 14, 47 -- Received: from EXHTCA01-CDC.nexus.csiro.au (2405:b000:601:13::247:21) by 14, 47 -- exhtca04-cdc.nexus.csiro.au (2405:b000:601:13::247:24) with Microsoft SMTP 14, 47 -- Server (TLS) id 14.3.158.1; Thu, 15 May 2014 10:55:54 +1000 14, 47 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80::9de3:d7af:4ed1:b7b6]) by 14, 47 -- exhtca01-cdc.nexus.csiro.au ([fe80::296a:b134:b85b:89de%13]) with mapi id 14, 47 -- 14.03.0158.001; Thu, 15 May 2014 10:55:54 +1000 14, 47 -- From: {Rosemary.White-at-csiro.au} 14, 47 -- To: {kalennon-at-hagerstowncc.edu} 14, 47 -- CC: {microscopy-at-microscopy.com} 14, 47 -- Subject: Re: [Microscopy] viaWWW:hand-held microtome 14, 47 -- Thread-Topic: [Microscopy] viaWWW:hand-held microtome 14, 47 -- Thread-Index: AQHPb9NSJyFARW/L1keE7RdjnH26k5tA0S8A 14, 47 -- Date: Thu, 15 May 2014 00:55:53 +0000 14, 47 -- Message-ID: {CF9A4AC2.151EBC%rosemary.white-at-csiro.au} 14, 47 -- In-Reply-To: {201405150017.s4F0H7MR001899-at-ns.microscopy.com} 14, 47 -- Accept-Language: en-NZ, en-US, en-AU 14, 47 -- Content-Language: en-US 14, 47 -- X-MS-Has-Attach: 14, 47 -- X-MS-TNEF-Correlator: 14, 47 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 14, 47 -- x-originating-ip: [152.83.195.117] 14, 47 -- Content-Type: text/plain; charset="us-ascii" 14, 47 -- Content-ID: {85EA60F2E4D2884CA3E1D70B11EEC36B-at-csiro.au} 14, 47 -- MIME-Version: 1.0 14, 47 -- Content-Transfer-Encoding: 8bit 14, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4F0ttxU025598 ==============================End of - Headers==============================
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==============================Original Headers============================== 27, 31 -- From frank_karl-at-ardl.com Thu May 15 07:00:57 2014 27, 31 -- Received: from cal1-mh675b.smtproutes.com (cal1-mh675b.smtproutes.com [208.70.89.128]) 27, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4FC0ueX028201 27, 31 -- for {microscopy-at-microscopy.com} ; Thu, 15 May 2014 07:00:57 -0500 27, 31 -- X-Katharion-ID: 1400155227.62378.cal1-mh675 27, 31 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by 27, 31 -- cal1-mh675.smtproutes.com [(192.69.16.47)] with ESMTP via TCP 27, 31 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 15 May 2014 12:00:27 +0000 27, 31 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 27, 31 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Thu, 15 27, 31 -- May 2014 08:00:27 -0400 27, 31 -- From: Frank Karl {frank_karl-at-ardl.com} 27, 31 -- To: "'Rosemary.White-at-csiro.au'" {Rosemary.White-at-csiro.au} 27, 31 -- CC: "Microscopy Listserver (microscopy-at-microscopy.com)" 27, 31 -- {microscopy-at-microscopy.com} 27, 31 -- Date: Thu, 15 May 2014 08:00:25 -0400 27, 31 -- Subject: RE: [Microscopy] Re: viaWWW:hand-held microtome 27, 31 -- Thread-Topic: [Microscopy] Re: viaWWW:hand-held microtome 27, 31 -- Thread-Index: Ac9v21l/8bWoYlHARl276UW27pFIygAWQqkg 27, 31 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772BFB1E7EFF-at-exchange2k7.ad.ardl.com} 27, 31 -- References: {201405150116.s4F1G7hU008813-at-ns.microscopy.com} 27, 31 -- In-Reply-To: {201405150116.s4F1G7hU008813-at-ns.microscopy.com} 27, 31 -- Accept-Language: en-US 27, 31 -- Content-Language: en-US 27, 31 -- X-MS-Has-Attach: 27, 31 -- X-MS-TNEF-Correlator: 27, 31 -- acceptlanguage: en-US 27, 31 -- Content-Type: text/plain; charset="us-ascii" 27, 31 -- MIME-Version: 1.0 27, 31 -- Content-Transfer-Encoding: 8bit 27, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4FC0ueX028201 ==============================End of - Headers==============================
I am a satisfied user of an FEI 650 tungsten instrument and find it quite capable for general work. Realize, though, that my definition of 'general' is rarely exceeding 30K magnification on mainly metallic fractures and various inorganic unknowns. It is apparent to me while examining my samples, though, that if I were to need higher magnification, a FEG would be much preferred; the W instrument is current and/or aberration limited at both high mags, low voltages, as well as high count rate EDX mapping. Having had two variable pressure scopes (other vendors) before the 650, I don't understand your comment about the FEI variable pressure being different from others; you may be referring to the secondary electron imaging based on Danilatos' ESEM detectors using electron cloud amplification. That is true. Also, realize the beam spreading effects in variable pressure mode severely restrict high magnification resolution in any scope. I would much prefer a FEG instrument but couldn't justify the extra $250K.
Hope this helps,
Rick
Richard A. Ross Sr. Metallurgist, Materials Engineering Allison Transmission Inc. Office: (317) 242-4880 - Fax (317) 242-7638 Richard.Ross-at-AllisonTransmission.com
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, May 09, 2014 6:43 PM To: formulaross-at-comcast.net Login Host: 204.195.66.114 Listserver Email Form V - 20120416 --------------------------------------------------------------------------- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to Microscopy-at-Microscopy.com
==============================Original Headers============================== 7, 37 -- From richard.ross-at-allisontransmission.com Thu May 15 08:16:43 2014 7, 37 -- Received: from IMR01.allisontransmission.com (imr01.allisontransmission.com [74.113.50.85]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4FDGgZa015940 7, 37 -- for {Microscopy-at-Microscopy.com} ; Thu, 15 May 2014 08:16:42 -0500 7, 37 -- X-ASG-Debug-ID: 1400159801-0388335f54e7faf0001-FOsErg 7, 37 -- Received: from USAT0SAW083.US.AD.ATI.INT ([10.1.14.52]) by IMR01.allisontransmission.com with ESMTP id huey6PoOpHvzhYnX (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Thu, 15 May 2014 09:16:41 -0400 (EDT) 7, 37 -- X-Barracuda-Envelope-From: richard.ross-at-allisontransmission.com 7, 37 -- X-ASG-Whitelist: Client 7, 37 -- Received: from USAT0SAW080.US.AD.ATI.INT ([192.168.1.69]) by 7, 37 -- USAT0SAW083.US.AD.ATI.INT ([10.1.14.52]) with mapi id 14.03.0158.001; Thu, 15 7, 37 -- May 2014 09:16:41 -0400 7, 37 -- From: "Richard A. Ross" {richard.ross-at-allisontransmission.com} 7, 37 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 7, 37 -- CC: "gary-at-gaugler.com" {gary-at-gaugler.com} 7, 37 -- Subject: FW: [Microscopy] viaWWW:FEI Quanta 600 ESEM 7, 37 -- Thread-Topic: [Microscopy] viaWWW:FEI Quanta 600 ESEM 7, 37 -- X-ASG-Orig-Subj: FW: [Microscopy] viaWWW:FEI Quanta 600 ESEM 7, 37 -- Thread-Index: AQHPa9gShqYWG4vPf0S9cb/Z+s5lYJtBnsuA 7, 37 -- Date: Thu, 15 May 2014 13:16:40 +0000 7, 37 -- Message-ID: {2BF09958126C994AB7C38B7B1247D94E46FDCDFC-at-USAT0SAW080} 7, 37 -- References: {201405092243.s49MhG7G019477-at-ns.microscopy.com} 7, 37 -- In-Reply-To: {201405092243.s49MhG7G019477-at-ns.microscopy.com} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.1.2.182] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-Barracuda-Connect: UNKNOWN[10.1.14.52] 7, 37 -- X-Barracuda-Start-Time: 1400159801 7, 37 -- X-Barracuda-Encrypted: AES128-SHA 7, 37 -- X-Barracuda-URL: http://74.113.50.85:8000/cgi-mod/mark.cgi 7, 37 -- X-Virus-Scanned: by bsmtpd at allisontransmission.com 7, 37 -- X-Barracuda-BRTS-Status: 1 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4FDGgZa015940 ==============================End of - Headers==============================
TESCAN USA Inc. is hiring three Field Service Engineers.
Positions are for Field Service Engineers based in the Northeast, Mid-Atlantic, and Southeast USA, to work out of their homes. The Field Service Engineer will be responsible for all aspects of customer service including routine maintenance, installations, emergency service, instrument troubleshooting and repair, customer training, and any necessary customer support associated with the position. The engineer will be required to be a self-starter and able to travel and work independently with minimal supervision. Good organizational skills and written and oral communication skills are a must. Maintaining good customer relations is essential.
Other requirements of the job include:
* Working knowledge of vacuum systems and components. * Working knowledge of Electron Microscope electron optics and image detectors. * Working knowledge of electronics and power supplies * Solid mechanical abilities to analyze complex and intricate mechanical components. * Computer skills of how PC's operate and Windows OS's from 2000 to Win 7 * Regularly required to crouch, crawl, stoop or kneel behind instrumentation in confined spaces * Must have ability to see, feel and handle small objects and use hand tools * Must have ability to lift 50 lbs. * Submit timely service reports and travel expense reports * If not US Citizen must have necessary authorization and documents to legally work in the USA * Must have valid driver's license * Must have valid Passport * Have a car in good condition capable of traveling several hundred miles if necessary * Must be able to pass a criminal and credit background check and drug testing.
TESCAN USA Inc. offers competitive salaries and benefits. Qualified candidates should send resume and cover letter along with salary requirements to info-at-tescan-usa.com and reference FSEMW. No phone calls will be accepted. Equal Opportunity Employer.
Colleen Leary Strategic Marketing Manager TESCAN USA 508 Thomson Park Dr. Cranberry Twp., PA 16066 Phone: 724-772-7433 E-mail: cleary-at-tescan-usa.com
==============================Original Headers============================== 13, 33 -- From cleary-at-tescan-usa.com Thu May 15 08:47:21 2014 13, 33 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0183.outbound.protection.outlook.com [207.46.163.183]) 13, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4FDlLwL003018 13, 33 -- for {Microscopy-at-Microscopy.Com} ; Thu, 15 May 2014 08:47:21 -0500 13, 33 -- Received: from CO2PR04MB779.namprd04.prod.outlook.com (10.141.227.144) by 13, 33 -- CO2PR04MB779.namprd04.prod.outlook.com (10.141.227.144) with Microsoft SMTP 13, 33 -- Server (TLS) id 15.0.944.11; Thu, 15 May 2014 13:47:19 +0000 13, 33 -- Received: from CO2PR04MB779.namprd04.prod.outlook.com ([10.141.227.144]) by 13, 33 -- CO2PR04MB779.namprd04.prod.outlook.com ([10.141.227.144]) with mapi id 13, 33 -- 15.00.0944.000; Thu, 15 May 2014 13:47:18 +0000 13, 33 -- From: Colleen Leary {cleary-at-tescan-usa.com} 13, 33 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 13, 33 -- Subject: Field Service Engineering Positions 13, 33 -- Thread-Topic: Field Service Engineering Positions 13, 33 -- Thread-Index: Ac9wRDJHOSFCOQY4R1Wivvlo4cTABA== 13, 33 -- Date: Thu, 15 May 2014 13:47:18 +0000 13, 33 -- Message-ID: {cdd7e74c5371446ba883f0c0bdc6324a-at-CO2PR04MB779.namprd04.prod.outlook.com} 13, 33 -- Accept-Language: en-US 13, 33 -- Content-Language: en-US 13, 33 -- X-MS-Has-Attach: 13, 33 -- X-MS-TNEF-Correlator: 13, 33 -- x-originating-ip: [24.154.112.76] 13, 33 -- x-forefront-prvs: 0212BDE3BE 13, 33 -- x-forefront-antispam-report: SFV:NSPM;SFS:(6009001)(428001)(252514010)(199002)(189002)(66066001)(74316001)(74662001)(31966008)(83072002)(2656002)(79102001)(87936001)(101416001)(64706001)(20776003)(76576001)(85852003)(54356999)(50986999)(77096999)(4396001)(99286001)(99396002)(83322001)(19580395003)(33646001)(19580405001)(77982001)(21056001)(92566001)(86362001)(46102001)(81342001)(81542001)(24736002);DIR:OUT;SFP:;SCL:1;SRVR:CO2PR04MB779;H:CO2PR04MB779.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 13, 33 -- received-spf: None (: tescan-usa.com does not designate permitted sender 13, 33 -- hosts) 13, 33 -- authentication-results: spf=none (sender IP is ) 13, 33 -- smtp.mailfrom=cleary-at-tescan-usa.com; 13, 33 -- Content-Type: text/plain; charset="us-ascii" 13, 33 -- MIME-Version: 1.0 13, 33 -- X-OriginatorOrg: tescan-usa.com 13, 33 -- Content-Transfer-Encoding: 8bit 13, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4FDlLwL003018 ==============================End of - Headers==============================
When I teach sectioning, I like another poster, use a carrot or Styrofoam to hold the sample with single edge razors to section. For educational and basic functions it works fine for the plant materials we used. However, I do have a hand microtome that was made in Japan and it came with a big knife similar to an old barbers straight edge blade. It is designed to hold paraffin blocks for sectioning. However, I have never managed to get around to using it since the materials we research are for the most part dried powdered botanicals or drug/dietary ingredient materials. I picked it up in case. From what I understand, the product is not being made anymore but I am sure someone out there sells them. Unfrotunately, I can't provide any feedback on the student hand microtome since I have not used it but it is made well and looks solid.
Sincerely,
James Neal-Kababick Director Flora Research Laboratories An FDA and DEA Registered & Inspected Laboratory Fellow AOAC International Adjunct Faculty Bastyr University Botanical Medicine Department 1-541-472-0980 phone jimk-at-floraresearch.com www.floraresearch.com
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-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, May 14, 2014 5:53 PM To: James Neal-Kababick
X-from: kalennon-at-hagerstowncc.edu ()
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Email: kalennon-at-hagerstowncc.edu Name: Kristen Lennon
Organization: Hagerstown Community College
Title-Subject: [Filtered] hand microtome
Message: Hi Fellow Microscopy Enthusiasts,
Do any of you have experience using a hand-held microtome? I have a colleague who is interested in purchasing one to use as part of an introduction to microscopy class for our undergraduates. Hand-held is not something I've ever used, and I'm a bit skeptical. I'd love to get her at least a vibratome, but I'd bet they are out of our current price range.
Any advice?
Thanks once again for your help, Kristen Lennon
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed May 14 18:55:19 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4ENtJdq010063 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 18:55:19 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 993E9700417 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 18:55:19 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id lzWusV9LnDnt for {microscopy-at-microscopy.com} ; 13, 28 -- Wed, 14 May 2014 18:55:15 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id B3AC370040A 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 18:55:15 -0500 (CDT) 13, 28 -- Message-ID: {53740263.4080505-at-microscopy.com} 13, 28 -- Date: Wed, 14 May 2014 18:55:15 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:hand-held microtome 13, 28 -- References: {201405140100.s4E10bGa012217-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201405140100.s4E10bGa012217-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201405140100.s4E10bGa012217-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 25, 30 -- From jimk-at-floraresearch.com Thu May 15 14:15:33 2014 25, 30 -- Received: from xsmtp07.mail2web.com (xsmtp07.mail2web.com [168.144.250.170]) 25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4FJFXb2027195 25, 30 -- for {Microscopy-at-Microscopy.com} ; Thu, 15 May 2014 14:15:33 -0500 25, 30 -- Received: from [10.5.31.9] (helo=hub03.ad2.softcom.biz) 25, 30 -- by xsmtp07.mail2web.com with esmtps (TLS-1.0:RSA_ARCFOUR_MD5:16) 25, 30 -- (Exim 4.63) 25, 30 -- (envelope-from {jimk-at-floraresearch.com} ) 25, 30 -- id 1Wl17n-0001RB-F1 25, 30 -- for Microscopy-at-Microscopy.com; Thu, 15 May 2014 15:15:32 -0400 25, 30 -- Received: from MBX74.ad2.softcom.biz ([10.5.13.37]) by hub03.ad2.softcom.biz 25, 30 -- ([10.5.31.9]) with mapi; Thu, 15 May 2014 15:15:26 -0400 25, 30 -- From: James Neal-Kababick {jimk-at-floraresearch.com} 25, 30 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 25, 30 -- Date: Thu, 15 May 2014 15:15:33 -0400 25, 30 -- Thread-Topic: hand-held microtome 25, 30 -- Thread-Index: Ac9v2vUAwKmhluzLRvyMIyhfwWcSewAllnNQ 25, 30 -- Message-ID: {143B8CF2094404458B4AA679F5A9877B6D9A6752BA-at-MBX74.ad2.softcom.biz} 25, 30 -- References: {201405150053.s4F0rBDb022553-at-ns.microscopy.com} 25, 30 -- In-Reply-To: {201405150053.s4F0rBDb022553-at-ns.microscopy.com} 25, 30 -- Accept-Language: en-US 25, 30 -- Content-Language: en-US 25, 30 -- X-MS-Has-Attach: 25, 30 -- X-MS-TNEF-Correlator: 25, 30 -- acceptlanguage: en-US 25, 30 -- Content-Type: text/plain; charset="us-ascii" 25, 30 -- MIME-Version: 1.0 25, 30 -- Subject: hand-held microtome 25, 30 -- Content-Transfer-Encoding: 8bit 25, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4FJFXb2027195 ==============================End of - Headers==============================
I have the prospect of doing TEM on some skin samples that have been embedded in Optimal Cutting Temperature medium and (presumably) plunge frozen. Knowing that skin can be tricky anyway for TEM fixation, and that plunge freezing 4 mm wide samples might result in horrible ice damage, I am feeling a bit apprehensive about what to expect.
I am grateful for any advice or experiences with OCT and TEM (even just a short reply).
1. Is it so likely to fail that it should not be attempted? 2. If it can be attempted, what should I expect (and describe) the likely quality as being? 3. Is there a protocol or any tricks (e.g., use edge or middle of 4 mm sample only, freeze substitution rather than thaw and fix etc.)?
Thanks Duane
AgResearch Limited | Lincoln Research Centre | New Zealand Dr Duane P Harland, Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz
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==============================Original Headers============================== 8, 35 -- From Duane.Harland-at-agresearch.co.nz Thu May 15 17:50:51 2014 8, 35 -- Received: from mx1.securemx.biz (mx1.securemx.biz [203.84.134.2]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4FMoo8J017966 8, 35 -- for {microscopy-at-microscopy.com} ; Thu, 15 May 2014 17:50:50 -0500 8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; d=nz.smxemail.com; s=alpha; c=relaxed/relaxed; 8, 35 -- q=dns/txt; i=-at-nz.smxemail.com; t=1400194249; 8, 35 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc; 8, 35 -- bh=EM8+zlI8gRcIbD7Csx1x/iLq+gzrLLL97t+YKvJD8z4=; 8, 35 -- b=FKPFO458AsCwmS0D7breM+95hr/rwdb4rYX6+MrHfW015os467PNwmY4tgnyicYI 8, 35 -- qRpRKQ8XoA3THTTrAaBt+BsrNBhWibbXb1d3BWR0bJsdGdDZNjXGPq8yS+Y8XNZF 8, 35 -- 4tNFPJSDp7Cp2dPQSheMuTgXVDi3i2aCNvu/2l5Kp8A=; 8, 35 -- Received: from invexcpv02.agresearch.co.nz ([202.20.102.50]) 8, 35 -- by omr.nz.smxemail.com with ESMTP (using TLSv1 8, 35 -- with cipher AES128-SHA (128/128 bits)) 8, 35 -- id 537544C8-D9642591-at-mta1103.omr; 8, 35 -- Thu, 15 May 2014 22:50:49 +0000 8, 35 -- Received: from INVEXCPV01.agresearch.co.nz ([169.254.1.18]) by 8, 35 -- invexcpv03.agresearch.co.nz ([147.158.130.6]) with mapi id 14.03.0174.001; 8, 35 -- Fri, 16 May 2014 10:50:46 +1200 8, 35 -- From: "Harland, Duane" {Duane.Harland-at-agresearch.co.nz} 8, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 35 -- Subject: OCT and TEM, any hope? 8, 35 -- Thread-Topic: OCT and TEM, any hope? 8, 35 -- Thread-Index: Ac9winWQ+qwUz0VGRBOlUSDiQrM09g== 8, 35 -- Date: Thu, 15 May 2014 22:50:44 +0000 8, 35 -- Message-ID: {C1CB2BCA7689064DA55A366147C8BEF157A6F9AB-at-invexcpv01.agresearch.co.nz} 8, 35 -- Accept-Language: en-GB, en-NZ, en-US 8, 35 -- Content-Language: en-US 8, 35 -- X-MS-Has-Attach: 8, 35 -- X-MS-TNEF-Correlator: 8, 35 -- x-originating-ip: [147.158.192.179] 8, 35 -- Content-Type: text/plain; charset="iso-8859-1" 8, 35 -- MIME-Version: 1.0 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4FMoo8J017966 ==============================End of - Headers==============================
Sorry to say so but I would guess your option 1 that it is "so likely to fail that it should not be attempted" is the right prediction. I have done a lot of quick-freezing with metal mirrors and even those specimens often fail. I have seen a ton of poorly frozen tissue and it isn't worth looking at. TEM is a great technique but it is too tedious and time-consuming to try unless you at least try to do the fixation in an optimal way. Plunge freezing is unlikely to be worth the effort. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: Duane.Harland-at-agresearch.co.nz [mailto:Duane.Harland-at-agresearch.co.nz] Sent: Thursday, May 15, 2014 5:52 PM To: Phillips, Thomas E.
Greetings microscopical friends,
I have the prospect of doing TEM on some skin samples that have been embedded in Optimal Cutting Temperature medium and (presumably) plunge frozen. Knowing that skin can be tricky anyway for TEM fixation, and that plunge freezing 4 mm wide samples might result in horrible ice damage, I am feeling a bit apprehensive about what to expect.
I am grateful for any advice or experiences with OCT and TEM (even just a short reply).
1. Is it so likely to fail that it should not be attempted? 2. If it can be attempted, what should I expect (and describe) the likely quality as being? 3. Is there a protocol or any tricks (e.g., use edge or middle of 4 mm sample only, freeze substitution rather than thaw and fix etc.)?
Thanks Duane
AgResearch Limited | Lincoln Research Centre | New Zealand Dr Duane P Harland, Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz
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==============================Original Headers============================== 8, 35 -- From Duane.Harland-at-agresearch.co.nz Thu May 15 17:50:51 2014 8, 35 -- Received: from mx1.securemx.biz (mx1.securemx.biz [203.84.134.2]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4FMoo8J017966 8, 35 -- for {microscopy-at-microscopy.com} ; Thu, 15 May 2014 17:50:50 -0500 8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; d=nz.smxemail.com; s=alpha; c=relaxed/relaxed; 8, 35 -- q=dns/txt; i=-at-nz.smxemail.com; t=1400194249; 8, 35 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc; 8, 35 -- bh=EM8+zlI8gRcIbD7Csx1x/iLq+gzrLLL97t+YKvJD8z4=; 8, 35 -- b=FKPFO458AsCwmS0D7breM+95hr/rwdb4rYX6+MrHfW015os467PNwmY4tgnyicYI 8, 35 -- qRpRKQ8XoA3THTTrAaBt+BsrNBhWibbXb1d3BWR0bJsdGdDZNjXGPq8yS+Y8XNZF 8, 35 -- 4tNFPJSDp7Cp2dPQSheMuTgXVDi3i2aCNvu/2l5Kp8A=; 8, 35 -- Received: from invexcpv02.agresearch.co.nz ([202.20.102.50]) 8, 35 -- by omr.nz.smxemail.com with ESMTP (using TLSv1 8, 35 -- with cipher AES128-SHA (128/128 bits)) 8, 35 -- id 537544C8-D9642591-at-mta1103.omr; 8, 35 -- Thu, 15 May 2014 22:50:49 +0000 8, 35 -- Received: from INVEXCPV01.agresearch.co.nz ([169.254.1.18]) by 8, 35 -- invexcpv03.agresearch.co.nz ([147.158.130.6]) with mapi id 14.03.0174.001; 8, 35 -- Fri, 16 May 2014 10:50:46 +1200 8, 35 -- From: "Harland, Duane" {Duane.Harland-at-agresearch.co.nz} 8, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 35 -- Subject: OCT and TEM, any hope? 8, 35 -- Thread-Topic: OCT and TEM, any hope? 8, 35 -- Thread-Index: Ac9winWQ+qwUz0VGRBOlUSDiQrM09g== 8, 35 -- Date: Thu, 15 May 2014 22:50:44 +0000 8, 35 -- Message-ID: {C1CB2BCA7689064DA55A366147C8BEF157A6F9AB-at-invexcpv01.agresearch.co.nz} 8, 35 -- Accept-Language: en-GB, en-NZ, en-US 8, 35 -- Content-Language: en-US 8, 35 -- X-MS-Has-Attach: 8, 35 -- X-MS-TNEF-Correlator: 8, 35 -- x-originating-ip: [147.158.192.179] 8, 35 -- Content-Type: text/plain; charset="iso-8859-1" 8, 35 -- MIME-Version: 1.0 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4FMoo8J017966 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 31 -- From PhillipsT-at-missouri.edu Thu May 15 17:56:33 2014 18, 31 -- Received: from um-tip1-missouri-out.um.umsystem.edu (um-tip1-missouri-out.um.umsystem.edu [198.209.49.135]) 18, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4FMuWLs025200 18, 31 -- for {microscopy-at-microscopy.com} ; Thu, 15 May 2014 17:56:32 -0500 18, 31 -- X-IronPort-Anti-Spam-Filtered: true 18, 31 -- X-IronPort-Anti-Spam-Result: Ag4FAN9EdVPPoJ7G/2dsb2JhbABWA4MGT1jFHwGBEhZ0giUBAQEEOhQiFQICAgEIEQMBAQELAhIJBxYbARQJCAIEAQkJCBUEiCANkFu7G4VAFwSMQBGBIScGCRILBwUCBAuDGoEVBIVLlUORV4M2bYEBQg 18, 31 -- X-IPAS-Result: Ag4FAN9EdVPPoJ7G/2dsb2JhbABWA4MGT1jFHwGBEhZ0giUBAQEEOhQiFQICAgEIEQMBAQELAhIJBxYbARQJCAIEAQkJCBUEiCANkFu7G4VAFwSMQBGBIScGCRILBwUCBAuDGoEVBIVLlUORV4M2bYEBQg 18, 31 -- Received: from um-tcas2.um.umsystem.edu ([207.160.158.198]) 18, 31 -- by um-tip1-exch-relay.um.umsystem.edu with ESMTP; 15 May 2014 17:56:31 -0500 18, 31 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.17]) by 18, 31 -- UM-TCAS2.um.umsystem.edu ([207.160.158.198]) with mapi id 14.03.0181.006; 18, 31 -- Thu, 15 May 2014 17:56:31 -0500 18, 31 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 18, 31 -- To: "Duane.Harland-at-agresearch.co.nz" {Duane.Harland-at-agresearch.co.nz} , 18, 31 -- "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 18, 31 -- Subject: RE: [Microscopy] OCT and TEM, any hope? 18, 31 -- Thread-Topic: [Microscopy] OCT and TEM, any hope? 18, 31 -- Thread-Index: AQHPcJA9dFMUqyKNnEKwYJCdvPXJqptCP7Ew 18, 31 -- Date: Thu, 15 May 2014 22:56:30 +0000 18, 31 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DDBDE912C-at-UM-MBX-T02.um.umsystem.edu} 18, 31 -- References: {201405152251.s4FMpdCU018802-at-ns.microscopy.com} 18, 31 -- In-Reply-To: {201405152251.s4FMpdCU018802-at-ns.microscopy.com} 18, 31 -- Accept-Language: en-US 18, 31 -- Content-Language: en-US 18, 31 -- X-MS-Has-Attach: 18, 31 -- X-MS-TNEF-Correlator: 18, 31 -- x-originating-ip: [128.206.81.115] 18, 31 -- Content-Type: text/plain; charset="us-ascii" 18, 31 -- MIME-Version: 1.0 18, 31 -- Content-Transfer-Encoding: 8bit 18, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4FMuWLs025200 ==============================End of - Headers==============================
From meds_popular10-at-prtcom.ru Thu May 15 21:38:08 2014 Return-Path: {meds_popular10-at-prtcom.ru} Received: from pppoe-46-226-70-014.prtcom.ru (pppoe-46-226-70-014.prtcom.ru [46.226.70.14]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4G2c3i8025895 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 15 May 2014 21:38:04 -0500 Message-Id: {201405160238.s4G2c3i8025895-at-ns.microscopy.com}
Good morning, dear Kristen and all,
perhaps the pdf via link http://www.modernmicroscopy.com/article_pix/040810_quarterblades/quarterblades.pdf will (TEETSOV Anna: Quarter-Razor blade for hand-sectioning) will contribute a little bit.
I recall a very old publication (technical note, 1960/70ies?) on a hand-made {serial cutting} instrument, where, several (full sized) razor-blades were mounted into a “holder construction” with at least two leading pins (as in wet shavers for men), up to 5 or 6 razor blades slipped over using kind of (really thin, according to the thickness of slices wanted) spacer/distance rings in between each razor blade, fixed at both ends and equipped with a handle. The “instrument” was fabricated either in a shop or was built as handicraft work. This way one would be able to “section” slices/slabs (like “micro-grossing” with a defined thickness [equidistant] from the original big specimen [from abalone to zysts (âş)]) Unfortunately I can’t find the article/sketch of that instrument, nevertheless I think this is worth to be mentioned.
Very best regards,
Wolfgang SALZBURG, AUSTRIA
Secretary of SCUR PLEASE VISIT: www.scur.org for the Program of 41st Annual SCUR(Society for Cutaneous Ultrastructure Research) -Meeting, 5-7 June 2104 in Monte Porzio Catone, ROME, Italy
====================== Von: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Gesendet: Donnerstag, 15. Mai 2014 02:07 An: MuĂź Wolfgang Betreff: [Microscopy] Hand-held microtome
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Hi Fellow Microscopy Enthusiasts, Do any of you have experience using a hand-held microtome? I have a colleague who is interested in purchasing one to use as part of an introduction to microscopy class for our undergraduates. Hand-held is not something I've ever used, and I'm a bit skeptical. I'd love to get her at least a vibratome, but I'd bet they are out of our current price range.
Any advice?
Thanks once again for your help, Kristen Lennon
Login Host: 96.239.156.15 Listserver Email Form V - 20120416 --------------------------------------------------------------------------- -- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to Microscopy-at-Microscopy.com ============================================ ==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed May 14 18:55:19 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4ENtJdq010063 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 18:55:19 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 993E9700417 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 18:55:19 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id lzWusV9LnDnt for {microscopy-at-microscopy.com} ; 13, 28 -- Wed, 14 May 2014 18:55:15 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id B3AC370040A 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2014 18:55:15 -0500 (CDT) 13, 28 -- Message-ID: {53740263.4080505-at-microscopy.com} 13, 28 -- Date: Wed, 14 May 2014 18:55:15 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:hand-held microtome 13, 28 -- References: {201405140100.s4E10bGa012217-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201405140100.s4E10bGa012217-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201405140100.s4E10bGa012217-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 15, 38 -- From W.Muss-at-salk.at Fri May 16 03:01:02 2014 15, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 15, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4G810VW030263 15, 38 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2014 03:01:01 -0500 15, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 15, 38 -- by hermes.salk.at (Postfix) with ESMTP id 1577A646B39 15, 38 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2014 10:00:57 +0200 (CEST) 15, 38 -- X-Scanned-By: SALK-Content-Filter 15, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 15, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 15, 38 -- with ESMTP id fr5RquDgbpHl for {microscopy-at-microscopy.com} ; 15, 38 -- Fri, 16 May 2014 10:00:56 +0200 (CEST) 15, 38 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 15, 38 -- by hermes.salk.at (Postfix) with ESMTP id D2824646B2F 15, 38 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2014 10:00:56 +0200 (CEST) 15, 38 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 15, 38 -- by N2EX199.lks.local with ESMTP; 16 May 2014 10:05:35 +0200 15, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 15, 38 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 15, 38 -- 14.02.0387.000; Fri, 16 May 2014 10:00:56 +0200 15, 38 -- From: =?utf-8?B?TXXDnyBXb2xmZ2FuZw==?= {W.Muss-at-salk.at} 15, 38 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 15, 38 -- Subject: [Microscopy] Re: Hand-held microtome 15, 38 -- Thread-Topic: [Microscopy] Re: Hand-held microtome 15, 38 -- Thread-Index: AQHPb9GZohAQfYW+YEOHc3r9uuiSe5tC1QyQ 15, 38 -- Date: Fri, 16 May 2014 08:00:55 +0000 15, 38 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D2D1F80-at-N2DT131.lksdom21.lks.local} 15, 38 -- References: {201405150006.s4F06n26023598-at-ns.microscopy.com} 15, 38 -- In-Reply-To: {201405150006.s4F06n26023598-at-ns.microscopy.com} 15, 38 -- Accept-Language: de-DE, en-US 15, 38 -- Content-Language: de-DE 15, 38 -- X-MS-Has-Attach: 15, 38 -- X-MS-TNEF-Correlator: 15, 38 -- x-originating-ip: [192.168.42.2] 15, 38 -- Content-Type: text/plain; charset="utf-8" 15, 38 -- MIME-Version: 1.0 15, 38 -- Content-Transfer-Encoding: 8bit 15, 38 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s4G810VW030263 ==============================End of - Headers==============================
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X-from: tprozoro-at-ameslab.gov ()
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Title-Subject: [Filtered] Postdoc in in-situ fluid cell imaging and analysis of biotemplated magnetic nanocrystals
Message: Greetings Everyone:
Our group is currently looking for a postdoctoral microscopist to work on characterization of biotemplated magnetic nanocrystals in-situ with the continuous flow fluid cell.
The position might require some travel to visit with collaborators. If you know anyone who may be interested, please suggest that they contact me. Thanks!
-Tanya Prozorov Emergent Atomic and Magnetic Structures Division of Materials Sciences and Engineering US DOE Ames Laboratory 332 Wilhelm Hall, Ames IA 50011 515-2943376 (office) 515-2941255 (lab) e-mail: tprozoro-at-ameslab.gov
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Dear All: I am helping to start out the cryo-tem in my Lab and is planning to train new users who need to use this technique. While generally i am familiar with the procedures in cryo-plunging, sample transfer from tem-box to Gatan cryo-holder in the cryo-workstation, i cannot perform such procedure if i am wearing a pair cryo-gloves becausae the gloves are thick and it is impossible use them to hold the tweezers and to pick up the copper grid in the pool of liquid nitrogen/liquid ethane.That would mean that my hands are not protected from the LN2 and this can be risky. My Insitute is very particular on safety and i need to conduct a Risk Assessment on this procedures. May i ask how safety is implemented during these procedures?
Another enquiry would be on the technical aspect during sample transferring. I notice that if i have to transfer the grid box from the dewar (during storage) to the Gatan workstation, the grid box will have to expose to the atmosphere where a rise in temperature and icing are expected. This can be minimized when i quickly move the grid box into the workstation. However, i find that it takes a longer time to transfer the gridbox from the workstation back to the conical tube or into the dewar. Will there a better solution to reduce the time for the gridbox to expose to the atmosphere and humidity?
There is a Cryo Grid Box Handling Tool given by Gatan, is it very useful in minimize temperature rise and humidity when transfering the gridbox from the Gatan workstation to the storage dewar/conical tube?
Thank you very much!
Cheers, Yee Yan, Tay FACTS Lab NTU, Singapore
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From medications_discounted3-at-mgts.by Sun May 18 09:25:52 2014 Return-Path: {medications_discounted3-at-mgts.by} Received: from mm-20-167-120-178.dynamic.pppoe.mgts.by (mm-20-167-120-178.dynamic.pppoe.mgts.by [178.120.167.20] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4IEPmm3032697 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 18 May 2014 09:25:49 -0500 Message-Id: {201405181425.s4IEPmm3032697-at-ns.microscopy.com}
Hi All
Here in the UK we have a health and safety clause called "best practicable means", this basically says if you cannot perform the action you may take another route. To handle cryo related components with sufficient dexterity is often made impossible using what would be called suitable gloves. In which case we would fall back on the clause, what happens in other countries?
I am responsible for making health and safety checks on car race tracks, so you may imagine the complex areas that have to be covered by the same clause.
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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-----Original Message----- X-from: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] Sent: 18 May 2014 06:12 To: protrain-at-emcourses.com
Dear All,
As the deadline for early registration for this year’s Microscopy and Microanalysis Meeting (Aug. 3-7 2014 in Hartford, CT) is coming closer, I would like to make you aware of our Sunday Short Course on 3D electron microscopy (description below). Please forward this also to your colleagues who may be interested in this topic. This course is ideal for people who need a comprehensive introduction or a refresher in 3D reconstruction methods and is suitable for biologists and material scientists. If you have already registered for the meeting you can add the course registration as “extra item only”.
X12 3D Electron Microscopy of Macromolecular Assemblies Teresa Ruiz, Michael Radermacher, Edward Morris 8:30 AM to 5:00 PM on Sunday, August 3.
• Sample preparation: deep stain, vitreous ice • Imaging conditions, low-dose imaging, tilt-pair data collection • Alignment techniques and multivariate statistical analysis • 3D reconstruction methods • X-ray structure docking • Techniques described have applications in both biological and material science
This short course will provide a comprehensive description of the methods used for 3D structure determination from electron micrographs of macromolecular complexes or weakly scattering specimens available in multiple copies. Specimen-preparation techniques for single particles (deep stain, vitreous ice) will be presented, followed by selection of optimal imaging conditions, including low-dose imaging. Next, a detailed explanation of image-processing techniques, with special emphasis on the random-conical reconstruction technique, will be presented. Finally, structure interpretation and docking of X-ray structures to 3D EM densities will be demonstrated. The techniques described could be applied to both biological and materials science specimens.
-- Michael Radermacher, Ph.D., Prof., FMSA University of Vermont Dept. Molecular Physiology and Biophysics HSRF 120 / 149 Beaumont Ave Burlington, VT 05405 USA
Tel: + 802 656-4834 Fax: + 802 656-0747
==============================Original Headers============================== 9, 22 -- From mraderma-at-uvm.edu Mon May 19 08:58:30 2014 9, 22 -- Received: from smtp2.uvm.edu (smtp2.uvm.edu [132.198.101.169]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4JDwUgL002769 9, 22 -- for {Microscopy-at-Microscopy.com} ; Mon, 19 May 2014 08:58:30 -0500 9, 22 -- Received: from webmail.uvm.edu (webmail1.uvm.edu [132.198.100.45]) 9, 22 -- by smtp2.uvm.edu (8.14.5/8.14.5) with ESMTP id s4JDwQSI011474 9, 22 -- for {Microscopy-at-Microscopy.com} ; Mon, 19 May 2014 09:58:26 -0400 9, 22 -- Received: from pool-64-222-120-59.burl.east.myfairpoint.net 9, 22 -- (pool-64-222-120-59.burl.east.myfairpoint.net [64.222.120.59]) by 9, 22 -- webmail.uvm.edu (Horde Framework) with HTTP; Mon, 19 May 2014 09:58:26 -0400 9, 22 -- Date: Mon, 19 May 2014 09:58:26 -0400 9, 22 -- Message-ID: {20140519095826.Horde.sinVA16adDBTeg4CLXXwh3A-at-webmail.uvm.edu} 9, 22 -- From: Michael Radermacher {mraderma-at-uvm.edu} 9, 22 -- To: Microscopy-at-Microscopy.com 9, 22 -- Subject: M&M 2014 Sunday Short Course on Electron Microscopy of 9, 22 -- Macromolecular Assemblies 9, 22 -- User-Agent: Internet Messaging Program (IMP) H4 (5.0.21) 9, 22 -- X-Authenticated-Sender: mraderma 9, 22 -- Content-Type: text/plain; charset=utf-8; format=flowed; DelSp=Yes 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Disposition: inline 9, 22 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I'm interested in hearing about your experiences with various cryoSEM "glues" for sticking down samples to holders prior to freezing, including pitfalls, advantages, disadvantages, etc. Also, for live insect or other invertebrate samples, what are your suggestions for immobilizing them so they can be stuck to the glue prior to freezing. Thanks for your advice.
Marie
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 6, 17 -- From marie.cantino-at-uconn.edu Mon May 19 13:32:04 2014 6, 17 -- Received: from mta2.uits.uconn.edu (mailscanner.uconn.edu [137.99.25.235]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4JIW4SL025738 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 May 2014 13:32:04 -0500 6, 17 -- Received: from d47h235.public.uconn.edu (d47h235.public.uconn.edu [137.99.47.235]) 6, 17 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id AC22AA2BF 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 May 2014 14:32:03 -0400 (EDT) 6, 17 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 6, 17 -- Content-Type: text/plain; charset=us-ascii 6, 17 -- Subject: SEM: cryo glue options 6, 17 -- Date: Mon, 19 May 2014 14:32:03 -0400 6, 17 -- Message-Id: {30CB659C-46C1-436B-951B-046E0ADE4798-at-uconn.edu} 6, 17 -- To: Microscopy-at-Microscopy.Com 6, 17 -- Mime-Version: 1.0 (Apple Message framework v1283) 6, 17 -- X-Mailer: Apple Mail (2.1283) 6, 17 -- Content-Transfer-Encoding: 8bit 6, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4JIW4SL025738 ==============================End of - Headers==============================
I was involved with the development of one of the first SEM cryo systems and since that time have always used Tissue Tec as my adhesive of choice.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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-----Original Message----- X-from: marie.cantino-at-uconn.edu [mailto:marie.cantino-at-uconn.edu] Sent: 19 May 2014 19:34 To: protrain-at-emcourses.com
We used to use TissueTek, but switched to Instrumedics Cryo-Gel because it's so much easier to handle. It's a gel, so doesn't run all over the place before being frozen. We use it a lot to arrange our samples precisely before cryostat sectioning. If there's a gel form of TissueTek I'm sure that'd be fine too.
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 20/05/14 4:43 AM, "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} wrote:
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From affordable_medications3-at-paleol.net Tue May 20 02:31:49 2014 Return-Path: {affordable_medications3-at-paleol.net} Received: from mail2.paleol.net (92.47.252.177.megaline.telecom.kz [92.47.252.177] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4K7Vjsi027941 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 20 May 2014 02:31:47 -0500 Message-Id: {201405200731.s4K7Vjsi027941-at-ns.microscopy.com}
I also use Tissue Tek but colloidal graphite also works well.
Regards Mark
Dr Mark A.E. Auty National Food Imaging Centre Food Chemistry & Technology Department Teagasc Food Research Centre Moorepark, Fermoy, Co. Cork Ireland
-----Original Message----- X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Sent: 19 May 2014 23:56 To: Mark Auty
We used to use TissueTek, but switched to Instrumedics Cryo-Gel because it's so much easier to handle. It's a gel, so doesn't run all over the place before being frozen. We use it a lot to arrange our samples precisely before cryostat sectioning. If there's a gel form of TissueTek I'm sure that'd be fine too.
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 20/05/14 4:43 AM, "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} wrote:
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==============================Original Headers============================== 27, 25 -- From Mark.Auty-at-teagasc.ie Tue May 20 03:31:07 2014 27, 25 -- Received: from exchange02.teagasc.ie (exchange02.teagasc.ie [137.191.243.71]) 27, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4K8V63X029123 27, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 May 2014 03:31:06 -0500 27, 25 -- Received: from HCVX322.teagasc.net ([fe80::c4d4:3aff:3bed:114b]) by 27, 25 -- HCVX324.teagasc.net ([::1]) with mapi id 14.03.0174.001; Tue, 20 May 2014 27, 25 -- 09:31:01 +0100 27, 25 -- From: Mark Auty {Mark.Auty-at-teagasc.ie} 27, 25 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 27, 25 -- Subject: FW: [Microscopy] Re: SEM: cryo glue options 27, 25 -- Thread-Topic: [Microscopy] Re: SEM: cryo glue options 27, 25 -- Thread-Index: AQHPc7WRzhODGC0Bm0OSJ5gIfhHtiJtJIwBw 27, 25 -- Date: Tue, 20 May 2014 08:31:00 +0000 27, 25 -- Message-ID: {55454099873F934DB9B1994639B840F1281A18E6-at-HCVX322.teagasc.net} 27, 25 -- References: {201405192256.s4JMuOvZ008904-at-ns.microscopy.com} 27, 25 -- In-Reply-To: {201405192256.s4JMuOvZ008904-at-ns.microscopy.com} 27, 25 -- Accept-Language: en-US, en-IE 27, 25 -- Content-Language: en-US 27, 25 -- X-MS-Has-Attach: 27, 25 -- X-MS-TNEF-Correlator: 27, 25 -- x-originating-ip: [10.9.88.237] 27, 25 -- Content-Type: text/plain; charset="utf-8" 27, 25 -- MIME-Version: 1.0 27, 25 -- Content-Transfer-Encoding: 8bit 27, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s4K8V63X029123 ==============================End of - Headers==============================
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Dear Jen, Steve , Guenter and Paul,
Thank you very much for your suggestions and recommendations. A consensus would be to avoid wearing gloves which does makes sense because it would be extremely dangerous if LN2 is trapped. On the other hand it would be interesting for me to check with my safety office if 'the best practicable means' exist in my country.
Guenter and Jen, if you didn't mind, could you send me the link to access the safety presentation as well as the picture for the modifid Nalgene bottle?
Cheers, Yee Yan FACTS Lab NTU
==============================Original Headers============================== 6, 34 -- From rongchigram79-at-yahoo.com.sg Tue May 20 07:53:16 2014 6, 34 -- Received: from nm20-vm6.bullet.mail.sg3.yahoo.com (nm20-vm6.bullet.mail.sg3.yahoo.com [106.10.149.133]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4KCrEk5021259 6, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 20 May 2014 07:53:15 -0500 6, 34 -- Received: from [106.10.166.115] by nm20.bullet.mail.sg3.yahoo.com with NNFMP; 20 May 2014 12:53:13 -0000 6, 34 -- Received: from [106.10.151.202] by tm4.bullet.mail.sg3.yahoo.com with NNFMP; 20 May 2014 12:53:13 -0000 6, 34 -- Received: from [127.0.0.1] by omp1014.mail.sg3.yahoo.com with NNFMP; 20 May 2014 12:53:13 -0000 6, 34 -- X-Yahoo-Newman-Property: ymail-3 6, 34 -- X-Yahoo-Newman-Id: 640639.81835.bm-at-omp1014.mail.sg3.yahoo.com 6, 34 -- Received: (qmail 94139 invoked by uid 60001); 20 May 2014 12:53:13 -0000 6, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1400590393; bh=JxaiOx42AvLNj3OoJN3V1fxt/7kPOyIOX6xQLLAcj3c=; h=Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=K8/1dJgyM4V74uSidGsKrlNXHNFthgRcRkxCby7GI//68q+jUTAtbzDMTlVMiUsE45XUr0UAjIFkeLSpXmpX+AEpuEFMRdQWyzAayEiJxUtJwIdivmc65TvRE1Yfz7Oii/WBJ/mQiNWxxAhSFOZq8oRJgO+zLPfnpdm0bhq9308= 6, 34 -- X-YMail-OSG: Aetb_UcVM1kTKDBMNzNBV_iFINXc4qyPwTFBIoB3MgzTHXb 6, 34 -- ONZ3tylXgmEzzgid2JsJ.xcc05.kMlSAP5Dt5Mu_7FD3sbUMNqHtG3NJU2Vz 6, 34 -- .tnr8F9MCmu8F72LVrVwtsx7UBO0ZUM97XsIpdn0Er8ghki.74wINBf8E192 6, 34 -- 7XibhbxugBQMLi6bAlbcL4fBYccv.r8yyuPjr2X_HYGIROwY2aJt0vph4OlQ 6, 34 -- FWR1LIcTE2UJxRwLdy_nwFon.lYOxeQUEvS2Yw.w.Vp1abfYz4Iq.Q2W7odF 6, 34 -- kqQxelUPityiVY1nhJoTD6W.3A3AvrAWEa5pDOaBuBOGSAhk6oLEkuDYthwh 6, 34 -- GbA7wKXgB3.BCnFcclaK7I_dDNiBILsyxVUkXSAtmOeN.xhGUOHp1FQEosED 6, 34 -- qvGAiwOs9HTcZ3wR7xlaz67YoNlzLFI3nmCJcogXFTdg0H.mI.l0txdiH5ho 6, 34 -- HElKsxuDcTZ8a2hOZZUJpj.PzIlITun1eo0Z7CaLdbPMWg_cBPJcZ8aajPvT 6, 34 -- 2lA-- 6, 34 -- Received: from [119.74.235.243] by web192506.mail.sg3.yahoo.com via HTTP; Tue, 20 May 2014 20:53:13 SGT 6, 34 -- X-Rocket-MIMEInfo: 002.001,RGVhciBKZW4sIFN0ZXZlICwgR3VlbnRlciBhbmQgUGF1bCwKClRoYW5rIHlvdSB2ZXJ5IG11Y2ggZm9yIHlvdXIgc3VnZ2VzdGlvbnMgYW5kIHJlY29tbWVuZGF0aW9ucy4gQSBjb25zZW5zdXMgd291bGQgYmUgdG8gYXZvaWQgd2VhcmluZyBnbG92ZXMgd2hpY2ggZG9lcyBtYWtlcyBzZW5zZSBiZWNhdXNlIGl0IHdvdWxkIGJlIGV4dHJlbWVseSBkYW5nZXJvdXMgaWYgTE4yIGlzIHRyYXBwZWQuIE9uIHRoZSBvdGhlciBoYW5kIGl0IHdvdWxkIGJlIGludGVyZXN0aW5nIGZvciBtZSB0byBjaGVjayB3aXRoIG0BMAEBAQE- 6, 34 -- X-Mailer: YahooMailWebService/0.8.188.663 6, 34 -- Message-ID: {1400590393.71783.YahooMailNeo-at-web192506.mail.sg3.yahoo.com} 6, 34 -- Date: Tue, 20 May 2014 20:53:13 +0800 (SGT) 6, 34 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 6, 34 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 6, 34 -- Subject: Cryo-TEM - Cryo-Safety/Risk Assessment and Other Questions 6, 34 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 6, 34 -- MIME-Version: 1.0 6, 34 -- Content-Type: text/plain; charset=utf-8 6, 34 -- Content-Transfer-Encoding: 8bit 6, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4KCrEk5021259 ==============================End of - Headers==============================
The Midwest Microscopy and Microanalysis Society will hold a meeting in conjunction with the University of Wisconsin-Madison on June 6, 2014. The program title is "In Situ Microscopy", and events also include a Facility Open House at the UW-Madison Materials Science Center on Thursday, June 5th. The open house schedule, a list of Friday's speakers and registration information can be found on our website Meetings page:
www.midwestmicroscopy.org
Please contact Ms. Kristy Wendt, kwendt3-at-wisc.edu, for registration and vendor information. We look forward to seeing you there!
Best regards,
Elaine Schumacher M3S Program Coordinator
********************************************************************* Elaine F. Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
Living and working in the desert SW, we have a number of researchers here looking at the effects of dust on the body (asthma, mine tailings, etc). I was recently asked if we could look at the dust using light microscopy.
Since I am a biologist by training, I have little experience with this kind of material. Can someone recommend some reference/reading material? I am probably not ready (yet) for a McCrone Research Institute course, but I am aware of their existence.
Thanks! Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
You can mount this material in a product called Cargille Meltmount 1.662 (other RI available too) which you can get from McCrone. It is available from McCrone and it is easy to use. There are instructions you can download or that will come with the medium. You can also do a wet mount in glycerol--water but the other mount is a "permanent" mount in that unless you keep the slide on edge in a very hot area, it will remain. The nice thing about meltmount is that you can reheat the slide and rotate the fibers or other specimen in the mountant for PLM. There is a great book that might be of value to you called Pharmaceutical Microscopy. Another fun book it the color Atals and Manual of Microscopy for Criminalists, Chemists and Conservators by CRC Press. This is kind of an interesting collection of techniques, applications and illustrations (lots of photos). There is a chapter on dust.
One other thing is that you can register on the McCrone site for free access to the particle atlas and view images of dust with details about the mounting technique and various images from optical light microscopy to EM. You can also purchase reference slides from McCrone or Wards of dust mounted in a medium like Meltmount.
There is a lot of interesting things to find in dust. A good way to practice is to collect some floor sweepings from the garage, kitchen or a public area for examination. I hope that this helps.
Sincerely,
James Neal-Kababick Director Flora Research Laboratories An FDA and DEA Registered & Inspected Laboratory Fellow AOAC International Adjunct Faculty Bastyr University Botanical Medicine Department 1-541-472-0980 phone jimk-at-floraresearch.com www.floraresearch.com
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-----Original Message----- X-from: dcromey-at-email.arizona.edu [mailto:dcromey-at-email.arizona.edu] Sent: Tuesday, May 20, 2014 8:14 AM To: James Neal-Kababick
Living and working in the desert SW, we have a number of researchers here looking at the effects of dust on the body (asthma, mine tailings, etc). I was recently asked if we could look at the dust using light microscopy.
Since I am a biologist by training, I have little experience with this kind of material. Can someone recommend some reference/reading material? I am probably not ready (yet) for a McCrone Research Institute course, but I am aware of their existence.
Thanks! Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
On May 17, 2014, at 10:21 PM, rongchigram79-at-yahoo.com.sg wrote:
} Dear All: I am helping to start out the cryo-tem in my Lab and is } planning to train new users who need to use this technique. While } generally i am familiar with the procedures in cryo-plunging, sample } transfer from tem-box to Gatan cryo-holder in the cryo-workstation, } i cannot perform such procedure if i am wearing a pair cryo-gloves } becausae the gloves are thick and it is impossible use them to hold } the tweezers and to pick up the copper grid in the pool of liquid } nitrogen/liquid ethane.That would mean that my hands are not } protected from the LN2 and this can be risky. My Insitute is very } particular on safety and i need to conduct a Risk Assessment on this } procedures. May i ask how safety is implemented during these } procedures? } } } Another enquiry would be on the technical aspect during sample } transferring. I notice that if i have to transfer the grid box from } the dewar (during storage) to the Gatan workstation, the grid box } will have to expose to the atmosphere where a rise in temperature } and icing are expected. This can be minimized when i quickly move } the grid box into the workstation. However, i find that it takes a } longer time to transfer the gridbox from the workstation back to the } conical tube or into the dewar. Will there a better solution to } reduce the time for the gridbox to expose to the atmosphere and } humidity? } } There is a Cryo Grid Box Handling Tool given by Gatan, is it very } useful in minimize temperature rise and humidity when transfering } the gridbox from the Gatan workstation to the storage dewar/conical } tube? } } Thank you very much! } } Cheers, } Yee Yan, Tay
Dear Tay, As others have said, trying to use cryo-gloves is bad practice. In fact, it is much more likely that you could be harmed trying to do the fine manipulations required while wearing these than without gloves. Liquid nitrogen (LN2) will not harm the skin unless it stays in contact for an extended time, but the same is not true of liquid ethane or propane. Occasionally small drops of this cryogen can be produced during the plunging process, and if they contact your skin you will see initially a white patch, typically ~1 mm in diameter, on the skin, which becomes a small blister. This is generally only an annoyance. A certain level of skill is required to manipulate the grids with cooled tweezers. If one leaves them cooling too long, the upper part can get too cold, but too little cooling of the tips can heat the grid. I have taught people to move quickly, but not to rush, when performing the necessary actions. When transferring a grid box, keep it upright so that some LN2 is present in the slots holding the grids. I usually use a large tweezer, ~20 cm long with ~2 mm wide tips, to grab the box either from the tube or station. Once again move quickly, but don't rush, and drop the box into the station or tube. If you want to remove a box from a tube containing other boxes, I use an intermediate holder. A styrofoam box ~10 cm x 10 cm x 3 cm deep is good for this. Dump out all the boxes from the tube, find the one you want, then put the others back. I have not had any problems with ice on either the grid boxes or the grids themselves using these procedures. Bear in mind that there is cold N2 vapor above the LN2, so anything in that layer will stay cold, but too rapid movement can mix the cold layer with room air; another reason not to rush your movements. I have found the old style covers, disks with a slot that can be rotated to uncover a slot or to keep them all covered, work better than the newer style "witches hat" covers. The newer covers often drag a grid out of its slot as they are unscrewed, and this is particularly problematic if the cover is opaque. It is also the case that the boxes with the disk covers are more compact, so more of them will fit into a tube. Our lab had a 13 mm punch, and we bought 30 cm x 30 cm sheets of an appropriate plastic--it has to be OK at LN2 temperature, and it is best if it is transparent. We punched out as many disks as we would need, drilled a central hole for a screw and another for the inner end of the slot, then made the rest of the slot with a Dremel tool. I prefer using steel screws to plastic ones, since I have broken a few plastic ones trying to loosen them. Yours, Bill
==============================Original Headers============================== 8, 34 -- From wtivol-at-sbcglobal.net Tue May 20 19:22:14 2014 8, 34 -- Received: from nm10-vm2.access.bullet.mail.bf1.yahoo.com (nm10-vm2.access.bullet.mail.bf1.yahoo.com [216.109.114.209]) 8, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4L0MDel014848 8, 34 -- for {microscopy-at-microscopy.com} ; Tue, 20 May 2014 19:22:13 -0500 8, 34 -- Received: from [66.196.81.161] by nm10.access.bullet.mail.bf1.yahoo.com with NNFMP; 21 May 2014 00:22:13 -0000 8, 34 -- Received: from [98.139.221.159] by tm7.access.bullet.mail.bf1.yahoo.com with NNFMP; 21 May 2014 00:22:13 -0000 8, 34 -- Received: from [127.0.0.1] by smtp119.sbc.mail.bf1.yahoo.com with NNFMP; 21 May 2014 00:22:13 -0000 8, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s1024; t=1400631733; bh=yK23plGw+Ho5/iB2Ckwl2eoStKNj+STcXn4BDbRRZKA=; h=X-Yahoo-Newman-Id:X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:Message-Id:From:To:In-Reply-To:Content-Type:Content-Transfer-Encoding:Mime-Version:Subject:Date:References:X-Mailer; b=KHCd5nLXohbmoXNKHx4O4rin8mN2QsrkWAceu1HWPHVQXv7zZuaA+dKy0kknvbiUO334xGaLRFEGlaY+7Kluw8HixBo3wBN6ht2LxvXZ440fYHRobeo08Ljti4fkNKZTq5Blf949AzZIYf6u+pmeynJoiCCmC01LPGezQlFiKjI= 8, 34 -- X-Yahoo-Newman-Id: 210467.12585.bm-at-smtp119.sbc.mail.bf1.yahoo.com 8, 34 -- X-Yahoo-Newman-Property: ymail-3 8, 34 -- X-YMail-OSG: XOcrVb4VM1mvCPDXQMp2z4.NwETs4tDYk7T3L2tw94biTpj 8, 34 -- .qTGSHhv_b2xOwDXLw4N8f2XcM0xmJOdrnEEcf2mLYM4zUh1itHCPnD2wxsA 8, 34 -- J0vSRaMwJ8eUndBqeN9.z.fKbdljRmGtC5H0M1K47b80mcuCtPxiIR_BtS0i 8, 34 -- ALOBjowGhq8i4TScjW0DRcs7q9LjbaPfDzA9QEyF39n9W8GMqqPcMYQpR_Le 8, 34 -- _ExgTrCrkNWUguLO5XU1dstZnD5VV8nuE.kNCatCzaYUq5qWgZorhcwI_80S 8, 34 -- sWqc0evYKrSXIvaqGHO6BsRCrP1LruKimubg4s0U8aF.cVLXFEAepkEhwC3K 8, 34 -- LstX9PSBOKBFaMELIGhaZagef2YX.XjnudIxxrvVOnWukMY4lg5Agya8Fksc 8, 34 -- kDhXkHYy3jFlU.r8hE6v33pKWYGf1zGQP4N554urG_CyxfwOyArVl8pbQiGF 8, 34 -- L3kV5Nn5NXksrLHqk1QhoF.B6Qy00uGBQOBqUgbG.uv_2GtEcm51SGBN3UE0 8, 34 -- 41IS7ZRoaioD1LkNeRLaOCAsdztxsT4_i.BjhSyYo 8, 34 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 8, 34 -- X-Rocket-Received: from [192.168.1.68] (wtivol-at-108.226.244.17 with plain [98.139.221.42]) 8, 34 -- by smtp119.sbc.mail.bf1.yahoo.com with SMTP; 21 May 2014 00:22:13 +0000 UTC 8, 34 -- Message-Id: {3B659F84-1CD4-48AD-A320-F81E7E43C591-at-sbcglobal.net} 8, 34 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 8, 34 -- To: rongchigram79-at-yahoo.com.sg, microscopy-at-microscopy.com 8, 34 -- In-Reply-To: {201405180521.s4I5LRDM008644-at-ns.microscopy.com} 8, 34 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 34 -- Content-Transfer-Encoding: 7bit 8, 34 -- Mime-Version: 1.0 (Apple Message framework v936) 8, 34 -- Subject: Re: [Microscopy] Cryo-TEM - Cryo-Safety/Risk Assessment and Other Questions 8, 34 -- Date: Tue, 20 May 2014 17:22:10 -0700 8, 34 -- References: {201405180521.s4I5LRDM008644-at-ns.microscopy.com} 8, 34 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
From drugs_popular14-at-tangeroutlet.com Tue May 20 19:51:14 2014 Return-Path: {drugs_popular14-at-tangeroutlet.com} Received: from mail4.tangeroutlet.com ([79.105.175.56]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4L0pBoA001680 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 20 May 2014 19:51:12 -0500 Message-Id: {201405210051.s4L0pBoA001680-at-ns.microscopy.com}
Greetings listers,
A colleague is looking to purchase a piezo-cooled stage for light microscopy, +/- 0.1C, range 2-35C. Any experiences or advice would be most welcome. Please contact me off list.
Thanks in advance,
Jim
-- James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Sorry, I had a neuron crossed somewhere. Meant to be Peltier-cooled. Too late in the day.
Jim
Greetings listers,
A colleague is looking to purchase a piezo-cooled stage for light microscopy, +/- 0.1C, range 2-35C. Any experiences or advice would be most welcome. Please contact me off list.
Thanks in advance,
Jim
-- James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
==============================Original Headers============================== 10, 19 -- From jehrman-at-mta.ca Wed May 21 19:02:39 2014 10, 19 -- Received: from smtpy.mta.ca (smtpy.mta.ca [138.73.1.86]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4M02cK3003843 10, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 21 May 2014 19:02:39 -0500 10, 19 -- Received: from mctnnbsa59w-156034038025.dhcp-dynamic.fibreop.nb.bellaliant.net ([156.34.38.25]:54497 helo=[192.168.2.14]) 10, 19 -- by smtpy.mta.ca with esmtpsa (TLSv1:DHE-RSA-AES128-SHA:128) 10, 19 -- (Exim 4.82) 10, 19 -- (envelope-from {jehrman-at-mta.ca} ) 10, 19 -- id 1WnGSV-0000CQ-21 10, 19 -- for Microscopy-at-microscopy.com; Wed, 21 May 2014 21:02:07 -0300 10, 19 -- Message-ID: {537D3E9B.20208-at-mta.ca} 10, 19 -- Date: Wed, 21 May 2014 21:02:35 -0300 10, 19 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 10, 19 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 10, 19 -- MIME-Version: 1.0 10, 19 -- To: Microscopy-at-microscopy.com 10, 19 -- Subject: piezo-cooled stage for light microscopy 10, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: zasad008-at-umn.edu Name: Joe Zasadzinski
Organization: University of Minnesota
Title-Subject: [Filtered] Confocal microscopes
Message: Does anyone have positive or negative experience with Bio Rad confocal microscopes and the E800 Nikon microscope? We are looking to buy a used confocal microscope, and we are trying to decide on the Bio Rad system.
Questions include ease of use, service, student friendly, etc?
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Email: vwporscherxr-at-gmail.com Name: Rod Rowland
Organization: MATSYS, Inc.
Title-Subject: [Filtered] How to diagnose 'no filament current' jeol 6100
Message: Anyone have suggestions on how to diagnose 'no filament current' on my vintage jeol 6100. Recently I relocated the SEM to a new facility and now after replacing the tungsten filament twice I still get no current on the filament current meter?? I'm just looking for a simple set of trouble shooting steps I can try.
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Dear Joe,
We have experience with a wide variety of optical microscopes and like many of the. Nikon Eclipse is a great stand.... definitely user friendly. Also, one which, to the best of our knowledge, has a good track record for stability and flexibility. Nikon has been especially energetic in its lens development program so if it doesn't come with all the objectives/eyepieces/condensers that you need for your particular experiments, there is a wide selection to choose from.
Re: BioRad You did not specify which model. When BioRad was in its hey-day, I was working with Sarastro, a main competitor. The biggest issue for you to check out is the alignment procedure. If it is one of the earlier models, the light path may be complex... OK if you have someone who can manage it, but not for novices. Also, see if you can run a sample. Look for over-exposure. The only other issue to check out is the nature of the pinholes. If you are doing co-localization studies, you want to make sure that there is some mechanism for setting the pinholes in front of each the"red" and "green" channels exactly the same. Otherwise, you end up taking information from different depths of field in each channel.
Hope this was helpful .. and Good Hunting! Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through Fall 2014. Call us today for a free training evaluation.
At 02:15 PM 5/23/2014, you wrote:
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I have purchased my first bag of sapphire discs to grow cells on for HPF. I am looking for advice on how to handle them. Do I need to clean them before using them? If so, how?
Then I would like to know if there is a trick for how to carbon coat them so that you get them in and out of the coater without losing them.
Does anyone have a trick for how to label or mark the discs so that you know what side the cells were grown on in case you drop the disc before it is frozen?
Thanks in advance for all suggestions.
Chris Christine A. Brantner, PhD [C] NIH NHLBI Electron Microscopy Core Facility National Institutes of Health 14 Service Road West Building 14E Room 111B MSC5570 Bethesda, MD 20892-5570
Hi Rod If you already have checked for broken filaments without improvement, it may be tricky and possibly expensive. 1. check all fuses, 2. check the HV cable using a insulation meter (megaohmmeter capable of 1000V) between both ends of the cable. It should read values below 1 Ohm. 3. If the cable is ok the HV-tank may be defect (happened to me after moving a J35c 100 m. Or the Load current control unit if you are lucky.
1&2 you can do yourselves, but you will at least need telephone advice or email advice for no 3 & 4 from a service technician. Erik
==============================Original Headers============================== 2, 34 -- From erikordell-at-icloud.com Fri May 23 18:06:36 2014 2, 34 -- Received: from st11p06mm-asmtp001.mac.com (st11p06mm-asmtp001.mac.com [17.172.124.249]) 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4NN6auB029681 2, 34 -- for {Microscopy-at-Microscopy.com} ; Fri, 23 May 2014 18:06:36 -0500 2, 34 -- MIME-version: 1.0 2, 34 -- Content-transfer-encoding: 7BIT 2, 34 -- Content-type: text/plain; CHARSET=US-ASCII 2, 34 -- Received: from [192.168.0.199] 2, 34 -- (c-8e6fe455.209-32-64736c10.cust.bredbandsbolaget.se [85.228.111.142]) 2, 34 -- by st11p06mm-asmtp001.mac.com 2, 34 -- (Oracle Communications Messaging Server 7u4-27.08(7.0.4.27.7) 64bit (built Aug 2, 34 -- 22 2013)) with ESMTPSA id {0N61000Z7UUPND50-at-st11p06mm-asmtp001.mac.com} for 2, 34 -- Microscopy-at-Microscopy.com; Fri, 23 May 2014 23:06:29 +0000 (GMT) 2, 34 -- From: Erik Ordell {erikordell-at-icloud.com} 2, 34 -- Date: Sat, 24 May 2014 01:06:24 +0200 2, 34 -- Subject: How to diagnose 'no filament current' jeol 6100 2, 34 -- To: Microscopy-at-Microscopy.com 2, 34 -- Message-id: {702D4266-8366-4E04-875E-7D71634473D0-at-icloud.com} 2, 34 -- X-Mailer: Apple Mail (2.1878.2) 2, 34 -- X-MANTSH: 1TEIXWV4bG1oaGkdHB0lGUkdDRl5PWBoaGREKTEMXGx0EGx0YBBIZBBsTEBseGh8 2, 34 -- aEQpYTRdLEQptfhcaEQpMWRcbGhsbEQpZSRcRClleF2hjeREKQ04XSxseGmJCH2lvHXV4GXhzB 2, 34 -- x5kGBkaHH4RClhcFxkEGgQbGwdNTh8YGBgZSwUbHQQbHRgEEhkEGxMQGx4aHxsRCl5ZF2FeckF 2, 34 -- 7EQpDWhcSHwQYGBIEGxsbBBseGBEKRFgXGBEKQkUXbWt+bEtsTnB/YEsRCkJOF2tFGlJQHkNcW 2, 34 -- VxoEQpCTBdiHlJZUkZpaERiAREKQmwXa29gf1sYX1ofT2IRCkJAF21TUh5hBQVIEhwcEQpwaBd 2, 34 -- 6Xlp8YlBZeU5pbhEKcGgXZ3p6ZURtGUx9S0kRCnBoF25ye2FJY2QcGh1rEQpwaBdpHEZcf3heT 2, 34 -- WIYQhEKcGgXemBARHJZTkV6Z3ARCnBsF3pofUFkX0xyRFJhEQ== 2, 34 -- X-CLX-Spam: false 2, 34 -- X-CLX-Score: 1011 2, 34 -- X-Proofpoint-Virus-Version: vendor=fsecure 2, 34 -- engine=2.50.10432:5.11.96,1.0.14,0.0.0000 2, 34 -- definitions=2014-05-23_07:2014-05-23,2014-05-23,1970-01-01 signatures=0 2, 34 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 2, 34 -- suspectscore=1 phishscore=0 adultscore=0 bulkscore=0 classifier=spam adjust=0 2, 34 -- reason=mlx scancount=1 engine=7.0.1-1402240000 definitions=main-1405230294 ==============================End of - Headers==============================
From affordable-pharmacy15-at-rinet.ru Fri May 23 18:07:48 2014 Return-Path: {affordable-pharmacy15-at-rinet.ru} Received: from h86-62-70-227.ln.rinet.ru (h86-62-70-227.ln.rinet.ru [86.62.78.227] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4NN7kSm030354 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 23 May 2014 18:07:47 -0500 Message-Id: {201405232307.s4NN7kSm030354-at-ns.microscopy.com}
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Email: lavoie-at-uw.edu Name: Ellen Lavoie
Organization: University of Washington NTUF
Title-Subject: [Filtered] embedding
Message: Anyone have any luck embedding samples for TEM- good or bad - that have been sitting in ethanol for 2-3 weeks.
Cheers, Ellen
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Title-Subject: [Filtered] Vacuum interlock fail on Edwards Scancoat 6
Message: When I turn on the vacuum pump the interlock light no longer comes on the sputter coater (I still hear a click like a valve is closing or such).
Is there a fix for this? Open up and blow off accumulated dust on a sensor?? Or is it replaceable?
It worked fine up till this point, only purchased in 1999.
thanks
Joe
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==============================Original Headers============================== 17, 40 -- From nestor.zaluzec-at-gmail.com Fri May 23 23:51:21 2014 17, 40 -- Received: from mail-pa0-f46.google.com (mail-pa0-f46.google.com [209.85.220.46]) 17, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4O4pKpl005459 17, 40 -- for {microscopy-at-microscopy.com} ; Fri, 23 May 2014 23:51:20 -0500 17, 40 -- Received: by mail-pa0-f46.google.com with SMTP id kq14so4976808pab.19 17, 40 -- for {microscopy-at-microscopy.com} ; Fri, 23 May 2014 21:51:20 -0700 (PDT) 17, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 40 -- d=gmail.com; s=20120113; 17, 40 -- h=sender:from:message-id:date:reply-to:user-agent:mime-version:to 17, 40 -- :subject:references:in-reply-to:content-type 17, 40 -- :content-transfer-encoding; 17, 40 -- bh=Ul/SJ4gUi7q6nYjmm8wvTHpe0qaOjF/Wt979a6DciSc=; 17, 40 -- b=Eqhd1YMvWzVL6J5EsH1bCvb1+fEV3ctG15QqpKD2KGhgSND3j0rwPcJop2Y6TkXOY5 17, 40 -- 03PwAzFC9fWGimUflpVwlnyt4b4GDLVnfNxCmYjMAYVUFR/WwoX2Zrh3n2QVFq7hjJNG 17, 40 -- 80EdTOhyHswd6u0y6bKonuvyoFtdGkxZ1+dSANbjHLBV64Qz+EwUieLt3r6k+Pq2NERI 17, 40 -- PLPe5L/WU+oB9cnQ7nkWnfpezX4NV4/F9W/TWfvH6R8YO07LicT6+eaGhMPPS4GJ8+Jw 17, 40 -- EhKL5ZZZP4kmcD38ZYHGUOk1+yTsWuAydwIOgJCtaqrlRB0mxWH5Efu91FtuRECCSYEq 17, 40 -- aZzg== 17, 40 -- X-Received: by 10.66.148.98 with SMTP id tr2mr11391879pab.33.1400907080205; 17, 40 -- Fri, 23 May 2014 21:51:20 -0700 (PDT) 17, 40 -- Received: from Nestors-MacBook-Pro-2014.local ([207.225.69.130]) 17, 40 -- by mx.google.com with ESMTPSA id ir10sm7211223pbc.59.2014.05.23.21.51.18 17, 40 -- for {microscopy-at-microscopy.com} 17, 40 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 17, 40 -- Fri, 23 May 2014 21:51:19 -0700 (PDT) 17, 40 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 17, 40 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 17, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 40 -- Message-ID: {53802545.9050806-at-microscopy.com} 17, 40 -- Date: Fri, 23 May 2014 21:51:17 -0700 17, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 17, 40 -- MIME-Version: 1.0 17, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 40 -- Subject: viaWWW:Vacuum interlock fail on Edwards Scancoat 6 17, 40 -- References: {201405231807.s4NI7Wj6020962-at-ns.microscopy.com} 17, 40 -- In-Reply-To: {201405231807.s4NI7Wj6020962-at-ns.microscopy.com} 17, 40 -- X-Forwarded-Message-Id: {201405231807.s4NI7Wj6020962-at-ns.microscopy.com} 17, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From drugs-canadian8-at-chello.pl Sat May 24 12:38:53 2014 Return-Path: {drugs-canadian8-at-chello.pl} Received: from 89-74-208-89.dynamic.chello.pl (89-74-208-89.dynamic.chello.pl [89.74.208.89]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4OHcpWH032351 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 24 May 2014 12:38:51 -0500 Message-Id: {201405241738.s4OHcpWH032351-at-ns.microscopy.com}
This may be too simplistic but could the bulb be burnt out?
zaluzec-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: joseph.uknalis-at-ars.usda.gov () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both joseph.uknalis-at-ars.usda.gov as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: joseph.uknalis-at-ars.usda.gov } Name: Joe Uknalis } } Title-Subject: [Filtered] Vacuum interlock fail on Edwards Scancoat 6 } } Message: When I turn on the vacuum pump the interlock light no longer } comes on the sputter coater (I still hear a click like a valve is } closing or such). } } Is there a fix for this? Open up and blow off accumulated dust on a } sensor?? Or is it replaceable? } } It worked fine up till this point, only purchased in 1999. } } thanks } } Joe } } } } } Login Host: 199.133.43.132 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- }
Please follow Nestor's instructions for Out of Office replies;
5.) Do not set your Email program to deliver "out of the office/vacation" messages. If you will be gone, you should unsubscribe from the list and subscribe again when you return.
Thank you
I received about 30 Out of Office replies. Not bad for 3000 users.
From canadian-drugstore15-at-mts-nn.ru Sun May 25 08:29:15 2014 Return-Path: {canadian-drugstore15-at-mts-nn.ru} Received: from 89-109-37-231.dynamic.mts-nn.ru (89-109-37-231.dynamic.mts-nn.ru [89.109.37.231]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4PDT7lK015032 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 25 May 2014 08:29:08 -0500 Message-Id: {201405251329.s4PDT7lK015032-at-ns.microscopy.com}
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Email: pa_grendys-at-fischione.com Name: Paula Grendys
Organization: E. A. Fischione Instruments, Inc.
Title-Subject: [Filtered] Fischione Instruments is hiring Field Service Engineer - Japan
Message: Field Service Engineer, Japan (in-country work location)
E. A. Fischione Instruments, Inc., located in Export, Pennsylvania, USA, is a world leader in microscopy and nanotechnology applications for the scientific community. In support of our ongoing international sales growth, we are seeking candidates for the position of Field Service Engineer-Japan to provide service to our clients located in Asia.
Located in-country Japan, and having the ability to work from your personal residence, this position requires strong self-initiative and the ability to work with little direct supervision. Relocation to Japan is available for qualified candidates.
In this key, customer-facing position, the Field Service Engineer-Japan is responsible for:
• Installing new equipment, executing product upgrades, and responding to service calls at client locations • Performing highly complex service and testing processes on miniature components, precision equipments, and complex instrumentation • Documenting service work, recording and analyzing test data, and preparing service reports • Promoting customer loyalty through proactive, professional communication. • Using company-supplied laptop to access technical and customer information • Placing inventory and parts orders • Providing information and feedback to Engineering and Production teams for problem identification & resolution • Providing comprehensive technical support by phone and email and promoting customer satisfaction through exemplary customer service orientation • Being "on-call" outside of normal working hours to provide emergency service.
Qualifications • Requires a degree in electronics, electromechanics, or a related discipline. • Must be able to write and speak English and Japanese. • Minimum of 5 years’ experience in a technical service capacity; prior field service responsibility for complex, precision, electronic and/or electromechanical devices is preferred. • Must possess the ability to interpret complex technical drawings, electronic schematics to the component level, and wiring diagrams. • Familiarity of standard vacuum practices and high-voltage technology is a plus. • Needs to take initiative and work independently, with a focus on results, quality, and customer service. • Understanding of computer networking is beneficial. • Excellent verbal and written communication skills are required. • Good organizational habits and outstanding attention to detail are essential. • Must have a valid driver’s license and acceptable driving record. • Must be willing to travel up to 50% of the time, in Japan and to nearby regions in Asia. • Must be able to pass government background checks in order to be admitted to some customer facilities.
Working conditions and physical requirements • Candidate will spend the majority of time in laboratory surroundings. • This job occasionally requires exerting up to 50 kilograms of force. • Must be able to sit, bend, kneel, and stoop; work may take place seated at a work station, on the floor, or on a step stool or ladder.
Please visit our Web site to learn more about our company at http://www.fischione.com See Career Opportunities for this position and link to apply online.
EOE
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon May 26 07:47:49 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4QClnoM030579 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 26 May 2014 07:47:49 -0500 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 73B18709F3F 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 26 May 2014 07:47:49 -0500 (CDT) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id hhK1lm50151O for {microscopy-at-microscopy.com} ; 19, 28 -- Mon, 26 May 2014 07:47:38 -0500 (CDT) 19, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id C1470709F2D 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 26 May 2014 07:47:38 -0500 (CDT) 19, 28 -- Message-ID: {538337EA.8050009-at-microscopy.com} 19, 28 -- Date: Mon, 26 May 2014 07:47:38 -0500 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:Fischione Instruments is hiring Field Service Engineer - Japan 19, 28 -- References: {201405251751.s4PHppqN019411-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201405251751.s4PHppqN019411-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201405251751.s4PHppqN019411-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 19, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pa_grendys-at-fischione.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pa_grendys-at-fischione.com Name: Paula Grendys
Organization: E. A. Fischione Instruments, Inc.
Title-Subject: [Filtered] Fischione Instruments is hiring Field Service Engineer - US
Message: Field Service Engineer, U.S. - PA
E. A. Fischione Instruments, Inc., located in Export, Pennsylvania, is a world leader in microscopy and nanotechnology applications for the scientific community.
In support of our ongoing sales growth, we are seeking candidates for the position of Field Service Engineer-US. This position requires strong self-initiative and the ability to work with little direct supervision.
In this key, customer-facing position, the Field Service Engineer-US is responsible for: • Scheduling and performing maintenance on scientific equipment at customer locations in accordance with service contract and company procedures and standards. Ensuring equipment meets performance specifications. • Evaluating, diagnosing, and repairing malfunctioning equipment in response to service calls. • Performing service on both ultra-precision subassemblies and complex instrumentation. • Working to extreme tolerances and both recognizing and taking the action necessary to achieve ultra-precise operation. • Performing highly complex service and testing processes, sometimes with miniature components. • Installing new equipment, executing product upgrades, and providing technical support. • Promoting customer satisfaction through proactive, professional communication. • Using company-supplied laptop to access technical and customer information. • Documenting service work, recording and analyzing test data, and preparing service reports. • Placing inventory and parts orders. • Providing comprehensive technical support by phone and email. • Being "on-call" outside of normal working hours to provide emergency service.
Qualifications • Requires an associate degree in electronics, electromechanics, or a related discipline. • Minimum of 5 years of experience in a technical service capacity; prior field service responsibility for complex, precision, electronic and/or electromechanical devices is preferred. • Must be able to interpret complex technical drawings, electronic schematics to the component level, and wiring diagrams. • Familiarity of standard vacuum practice and high-voltage technology is a plus. • Needs to take initiative and work independently with a focus on results, quality, and customer service. • Understanding of computer networking is beneficial. • Excellent verbal and written communication skills are required. • Good organizational habits and outstanding attention to detail are essential. • Must have a valid driver’s license and acceptable driving record. • Must be willing to travel up to 50% of the time, both domestically and internationally. • Must be able to pass federal background checks in order to be admitted to some customer facilities.
Working conditions and physical requirements • Candidate will spend the majority of time in laboratory surroundings. • This job occasionally requires exerting up to 110 pounds of force. • Must be able to sit, bend, kneel, and stoop; work may take place seated at a work station, on the floor, or on a step stool or ladder.
Please visit our Web site to learn more about our company at http://www.fischione.com See Career Opportunities for this position and link to apply online.
EOE
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==============================Original Headers============================== 25, 29 -- From microscopylistserver-noreply-at-microscopy.com Mon May 26 07:48:18 2014 25, 29 -- Received: from znl.com ([206.69.208.20]) 25, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4QCmIiZ030858 25, 29 -- for {microscopy-at-microscopy.com} ; Mon, 26 May 2014 07:48:18 -0500 25, 29 -- Received: from localhost (localhost [127.0.0.1]) 25, 29 -- by znl.com (Postfix) with ESMTP id 6E804709F4D 25, 29 -- for {microscopy-at-microscopy.com} ; Mon, 26 May 2014 07:48:18 -0500 (CDT) 25, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 25, 29 -- Received: from znl.com ([127.0.0.1]) 25, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 25, 29 -- with ESMTP id FqiG3fhJRJBQ for {microscopy-at-microscopy.com} ; 25, 29 -- Mon, 26 May 2014 07:48:13 -0500 (CDT) 25, 29 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 25, 29 -- by znl.com (Postfix) with ESMTPA id BCAE6709F40 25, 29 -- for {microscopy-at-microscopy.com} ; Mon, 26 May 2014 07:48:13 -0500 (CDT) 25, 29 -- Message-ID: {5383380D.6070807-at-microscopy.com} 25, 29 -- Date: Mon, 26 May 2014 07:48:13 -0500 25, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 25, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 25, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 25, 29 -- MIME-Version: 1.0 25, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 25, 29 -- Subject: viaWWW:Fischione Instruments is hiring Field Service Engineer - US,,Message: 25, 29 -- Field Service Engineer, U.S. - PA 25, 29 -- References: {201405251756.s4PHuGL3019447-at-ns.microscopy.com} 25, 29 -- In-Reply-To: {201405251756.s4PHuGL3019447-at-ns.microscopy.com} 25, 29 -- X-Forwarded-Message-Id: {201405251756.s4PHuGL3019447-at-ns.microscopy.com} 25, 29 -- Content-Type: text/plain; charset=UTF-8; format=flowed 25, 29 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From popular-meds5-at-airtelbroadband.in Mon May 26 11:33:42 2014 Return-Path: {popular-meds5-at-airtelbroadband.in} Received: from ABTS-North-Dynamic-075.2.162.122.airtelbroadband.in (ABTS-North-Dynamic-001.136.162.122.airtelbroadband.in [122.162.136.1] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4QGXZHr009499 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 26 May 2014 11:33:37 -0500 Message-Id: {201405261633.s4QGXZHr009499-at-ns.microscopy.com}
X-from: alexandre.bastien-at-fsaa.ulaval.ca ()
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I got a large number of BF image that needed to be analyzed in a full automatic way. Our images covers a strongly different from one another. Here are some samples:
I need to do a cell segmentation, evaluate cell area, interstitial area, nucleus position relative to the closest cell border, and maybe more.
I've tried severeal algorithm in Matlab and ImageJ, but none of them seems to cover all of our image types, and analysis needs.
Do you know a software that can analyze this automatically ?
Many thanks.
Alexandre
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The main thing you need to do is capture the images appropriately so they can be analysed. It's always more efficient to put the effort into good image capture. Then the software can deal with the images quite easily. But if you have to work with images like the ones in your email rather than re-take them, you may have to do quite a bit of non-automated work, unless you have access to rather sophisticated analysis software.
I would first do shading correction and then you may be able to play around with segmentation based on hue/colour, and detection of outlines by intensity gradients. Others on this list with much more analysis experience may be able to provide more detailed advice.
good luck, Rosemary White
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
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From drugstore-canadian14-at-abt-inc.com Tue May 27 06:15:45 2014 Return-Path: {drugstore-canadian14-at-abt-inc.com} Received: from mail4.abt-inc.com ([90.151.95.81]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4RBFf2x000634 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 27 May 2014 06:15:42 -0500 Message-Id: {201405271115.s4RBFf2x000634-at-ns.microscopy.com}
Greetings,
Although I have not fully automated the protocol, I have used CellSens (which I am new to using) which I got with my microscope and it makes analysis of distances and areas very simple and rapid. What used to take me hours to do with my ocular micrometer is done in minutes now. I have been able to rapidly determine mean areas for fungal spores as well as measurement of mean spore width and height and take it into excel with a few mouse clicks. I would say that I have to agree with Dr. White in that image quality will impact the ease of use of any package as well as the ability to manually measure with a program (versus fully automated analysis of images).
Sincerely,
James Neal-Kababick Director Flora Research Laboratories An FDA and DEA Registered & Inspected Laboratory Fellow AOAC International Adjunct Faculty Bastyr University Botanical Medicine Department 1-541-472-0980 phone jimk-at-floraresearch.com www.floraresearch.com
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-----Original Message----- X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Sent: Tuesday, May 27, 2014 12:36 AM To: James Neal-Kababick
Dear Alexandre,
The main thing you need to do is capture the images appropriately so they can be analysed. It's always more efficient to put the effort into good image capture. Then the software can deal with the images quite easily. But if you have to work with images like the ones in your email rather than re-take them, you may have to do quite a bit of non-automated work, unless you have access to rather sophisticated analysis software.
I would first do shading correction and then you may be able to play around with segmentation based on hue/colour, and detection of outlines by intensity gradients. Others on this list with much more analysis experience may be able to provide more detailed advice.
good luck, Rosemary White
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 27/05/14 2:22 PM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
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==============================Original Headers============================== 11, 46 -- From prvs=217a9ec76=Rosemary.White-at-csiro.au Tue May 27 02:08:42 2014 11, 46 -- Received: from act-MTAout5.csiro.au (act-MTAout5.csiro.au [150.229.7.42]) 11, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4R78frm003461 11, 46 -- for {microscopy-at-microscopy.com} ; Tue, 27 May 2014 02:08:41 -0500 11, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 11, 46 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; 11, 46 -- t=1401174522; x=1432710522; 11, 46 -- h=from:to:subject:date:message-id:in-reply-to:content-id: 11, 46 -- content-transfer-encoding:mime-version; 11, 46 -- bh=+OKkQJSD035sFbQPbzjRsFKr+ATTH8KsJ2lveLruZG4=; 11, 46 -- b=AkRbSs+T+xevl5tQh8vtaDj1gLMZhJZKXNm4Nn3d6QFKGSkJDOC7prNa 11, 46 -- y1zk8gSceJbDX1rzef+ALf33JHLsYrb1WJdtLqCULvkz3RXOMX6E2zXqZ 11, 46 -- J9F8vQ7XxupYDX7; 11, 46 -- X-SBRS: 5.3 11, 46 -- X-IronPort-Anti-Spam-Filtered: true 11, 46 -- X-IronPort-Anti-Spam-Result: AusZAJs5hFOYU/cYmWdsb2JhbABZgmV0WKUoCgGZJ4FqgVEBgQsWDgEBAQEBCAsLBxQogiUBBk49AQgRAwECDQoBSAEUCQgCBAEJCR0EiCEBDMcijGwXjEcRgRgRAR06BgwBhC0EiWeQDIE9gnSHZYpJbIEDBxci 11, 46 -- X-IPAS-Result: AusZAJs5hFOYU/cYmWdsb2JhbABZgmV0WKUoCgGZJ4FqgVEBgQsWDgEBAQEBCAsLBxQogiUBBk49AQgRAwECDQoBSAEUCQgCBAEJCR0EiCEBDMcijGwXjEcRgRgRAR06BgwBhC0EiWeQDIE9gnSHZYpJbIEDBxci 11, 46 -- X-IronPort-AV: E=Sophos;i="4.98,917,1392123600"; 11, 46 -- d="scan'208";a="119672255" 11, 46 -- Received: from exhtca04-cdc.nexus.csiro.au ([152.83.247.24]) 11, 46 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES128-SHA; 27 May 2014 17:08:39 +1000 11, 46 -- Received: from EXHTCA01-CDC.nexus.csiro.au (2405:b000:601:13::247:21) by 11, 46 -- exhtca04-cdc.nexus.csiro.au (2405:b000:601:13::247:24) with Microsoft SMTP 11, 46 -- Server (TLS) id 14.3.158.1; Tue, 27 May 2014 17:08:39 +1000 11, 46 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80::9de3:d7af:4ed1:b7b6]) by 11, 46 -- exhtca01-cdc.nexus.csiro.au ([fe80::296a:b134:b85b:89de%13]) with mapi id 11, 46 -- 14.03.0158.001; Tue, 27 May 2014 17:08:38 +1000 11, 46 -- From: {Rosemary.White-at-csiro.au} 11, 46 -- To: {alexandre.bastien-at-fsaa.ulaval.ca} , {microscopy-at-microscopy.com} 11, 46 -- Subject: Re: [Microscopy] viaWWW:Brightfield image analysis 11, 46 -- Thread-Topic: [Microscopy] viaWWW:Brightfield image analysis 11, 46 -- Thread-Index: AQHPeWNu/X6eE6xB0kaeugsfscBcKptUAi6A 11, 46 -- Date: Tue, 27 May 2014 07:08:39 +0000 11, 46 -- Message-ID: {CFAA7568.152DC3%rosemary.white-at-csiro.au} 11, 46 -- In-Reply-To: {201405270422.s4R4MUAb022612-at-ns.microscopy.com} 11, 46 -- Accept-Language: en-NZ, en-US, en-AU 11, 46 -- Content-Language: en-US 11, 46 -- X-MS-Has-Attach: 11, 46 -- X-MS-TNEF-Correlator: 11, 46 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 11, 46 -- x-originating-ip: [152.83.195.117] 11, 46 -- Content-Type: text/plain; charset="iso-8859-1" 11, 46 -- Content-ID: {3F7526DA7A5BBE4E9B40A213CCF2D3A8-at-csiro.au} 11, 46 -- MIME-Version: 1.0 11, 46 -- Content-Transfer-Encoding: 8bit 11, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4R78frm003461 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 30 -- From jimk-at-floraresearch.com Tue May 27 11:25:57 2014 23, 30 -- Received: from xsmtp07.mail2web.com (xsmtp07.mail2web.com [168.144.250.170]) 23, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4RGPvwm009187 23, 30 -- for {Microscopy-at-Microscopy.com} ; Tue, 27 May 2014 11:25:57 -0500 23, 30 -- Received: from [10.5.31.11] (helo=hub04.ad2.softcom.biz) 23, 30 -- by xsmtp07.mail2web.com with esmtps (TLS-1.0:RSA_ARCFOUR_MD5:16) 23, 30 -- (Exim 4.63) 23, 30 -- (envelope-from {jimk-at-floraresearch.com} ) 23, 30 -- id 1WpKCH-0000qJ-S7 23, 30 -- for Microscopy-at-Microscopy.com; Tue, 27 May 2014 12:25:57 -0400 23, 30 -- Received: from MBX74.ad2.softcom.biz ([10.5.13.37]) by hub04.ad2.softcom.biz 23, 30 -- ([10.5.31.11]) with mapi; Tue, 27 May 2014 12:25:51 -0400 23, 30 -- From: James Neal-Kababick {jimk-at-floraresearch.com} 23, 30 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 23, 30 -- Date: Tue, 27 May 2014 12:25:49 -0400 23, 30 -- Thread-Topic: [Microscopy] Re: viaWWW:Brightfield image analysis 23, 30 -- Thread-Index: Ac95fkLPVsryDHR/SduObtcZN04wjgASQWfg 23, 30 -- Message-ID: {143B8CF2094404458B4AA679F5A9877B6D9A675946-at-MBX74.ad2.softcom.biz} 23, 30 -- References: {201405270735.s4R7ZYas022513-at-ns.microscopy.com} 23, 30 -- In-Reply-To: {201405270735.s4R7ZYas022513-at-ns.microscopy.com} 23, 30 -- Accept-Language: en-US 23, 30 -- Content-Language: en-US 23, 30 -- X-MS-Has-Attach: 23, 30 -- X-MS-TNEF-Correlator: 23, 30 -- acceptlanguage: en-US 23, 30 -- Content-Type: text/plain; charset="iso-8859-1" 23, 30 -- MIME-Version: 1.0 23, 30 -- Subject: RE: [Microscopy] Re: viaWWW:Brightfield image analysis 23, 30 -- Content-Transfer-Encoding: 8bit 23, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4RGPvwm009187 ==============================End of - Headers==============================
I have resurrected an ancient Buehler Vibromet polisher (model 67-1517) and would like to use it to put a final polish on probe samples. I have been unable to find cloths or abrasive pads for it (12-inch model) and wonder if anyone has experience with this polisher.
Nick
Nicholas J. Foster SENCR-MIC Laboratory Technician Fayetteville State University Office: (910) 672-2039 Probe Lab: (910) 672-2037 Email: nfoster1-at-uncfsu.edu
==============================Original Headers============================== 4, 33 -- From nfoster1-at-uncfsu.edu Tue May 27 13:28:38 2014 4, 33 -- Received: from mx0a-000c3a01.pphosted.com (mx0a-000c3a01.pphosted.com [208.84.65.195]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4RISbY0029833 4, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 13:28:38 -0500 4, 33 -- Received: from pps.filterd (m0001660 [127.0.0.1]) 4, 33 -- by mx0a-000c3a01.pphosted.com (8.14.5/8.14.5) with SMTP id s4RIRx6x028654 4, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 14:28:37 -0400 4, 33 -- Received: from exchangeweb.uncfsu.edu ([152.6.0.95]) 4, 33 -- by mx0a-000c3a01.pphosted.com with ESMTP id 1m4crmaucu-1 4, 33 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 4, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 14:28:37 -0400 4, 33 -- Received: from FSUMBX.uncfsu.edu ([fe80::f97c:1637:423b:a461]) by 4, 33 -- fsumtb1a.uncfsu.edu ([2002:9806:55f::9806:55f]) with mapi; Tue, 27 May 2014 4, 33 -- 14:28:35 -0400 4, 33 -- From: "Foster, Nicholas" {nfoster1-at-uncfsu.edu} 4, 33 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 33 -- Date: Tue, 27 May 2014 14:28:35 -0400 4, 33 -- Subject: Buehler Vibromet polisher help. 4, 33 -- Thread-Topic: Buehler Vibromet polisher help. 4, 33 -- Thread-Index: AQHPedl4CEsLXJ2IQkOjxqHwjE7Ocg== 4, 33 -- Message-ID: {F2FA8078BCFC0B49AD95B1B3CD75567507A926A9EA-at-FSUMBX.uncfsu.edu} 4, 33 -- Accept-Language: en-US 4, 33 -- Content-Language: en-GB 4, 33 -- X-MS-Has-Attach: 4, 33 -- X-MS-TNEF-Correlator: 4, 33 -- acceptlanguage: en-US 4, 33 -- Content-Type: text/plain; charset="us-ascii" 4, 33 -- MIME-Version: 1.0 4, 33 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 4, 33 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 4, 33 -- engine=7.0.1-1402240000 definitions=main-1405270250 4, 33 -- Content-Transfer-Encoding: 8bit 4, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4RISbY0029833 ==============================End of - Headers==============================
Are you sure you want to do that? What kind of samples do you process?
My experience is that vibratory polishing with colloidal silica can lead to topography that causes problems rather than resolving problems. Differences in hardness can lead to polishing relief. It can also chemically etch some samples. And there can also be a problem of silica residue on the sample after polishing if the cleaning protocol is not thorough enough. It is quite difficult to remove those deposits once they form.
Warren Straszheim ________________________________________ X-from: nfoster1-at-uncfsu.edu {nfoster1-at-uncfsu.edu} Sent: Tuesday, May 27, 2014 1:29 PM To: Straszheim, Warren E [BIOTC]
Via Allen Glazner at UNC
I have resurrected an ancient Buehler Vibromet polisher (model 67-1517) and would like to use it to put a final polish on probe samples. I have been unable to find cloths or abrasive pads for it (12-inch model) and wonder if anyone has experience with this polisher.
Nick
Nicholas J. Foster SENCR-MIC Laboratory Technician Fayetteville State University Office: (910) 672-2039 Probe Lab: (910) 672-2037 Email: nfoster1-at-uncfsu.edu
==============================Original Headers============================== 4, 33 -- From nfoster1-at-uncfsu.edu Tue May 27 13:28:38 2014 4, 33 -- Received: from mx0a-000c3a01.pphosted.com (mx0a-000c3a01.pphosted.com [208.84.65.195]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4RISbY0029833 4, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 13:28:38 -0500 4, 33 -- Received: from pps.filterd (m0001660 [127.0.0.1]) 4, 33 -- by mx0a-000c3a01.pphosted.com (8.14.5/8.14.5) with SMTP id s4RIRx6x028654 4, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 14:28:37 -0400 4, 33 -- Received: from exchangeweb.uncfsu.edu ([152.6.0.95]) 4, 33 -- by mx0a-000c3a01.pphosted.com with ESMTP id 1m4crmaucu-1 4, 33 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 4, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 14:28:37 -0400 4, 33 -- Received: from FSUMBX.uncfsu.edu ([fe80::f97c:1637:423b:a461]) by 4, 33 -- fsumtb1a.uncfsu.edu ([2002:9806:55f::9806:55f]) with mapi; Tue, 27 May 2014 4, 33 -- 14:28:35 -0400 4, 33 -- From: "Foster, Nicholas" {nfoster1-at-uncfsu.edu} 4, 33 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 33 -- Date: Tue, 27 May 2014 14:28:35 -0400 4, 33 -- Subject: Buehler Vibromet polisher help. 4, 33 -- Thread-Topic: Buehler Vibromet polisher help. 4, 33 -- Thread-Index: AQHPedl4CEsLXJ2IQkOjxqHwjE7Ocg== 4, 33 -- Message-ID: {F2FA8078BCFC0B49AD95B1B3CD75567507A926A9EA-at-FSUMBX.uncfsu.edu} 4, 33 -- Accept-Language: en-US 4, 33 -- Content-Language: en-GB 4, 33 -- X-MS-Has-Attach: 4, 33 -- X-MS-TNEF-Correlator: 4, 33 -- acceptlanguage: en-US 4, 33 -- Content-Type: text/plain; charset="us-ascii" 4, 33 -- MIME-Version: 1.0 4, 33 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 4, 33 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 4, 33 -- engine=7.0.1-1402240000 definitions=main-1405270250 4, 33 -- Content-Transfer-Encoding: 8bit 4, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4RISbY0029833 ==============================End of - Headers==============================
==============================Original Headers============================== 10, 35 -- From wesaia-at-iastate.edu Tue May 27 14:31:32 2014 10, 35 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2lp0212.outbound.protection.outlook.com [207.46.163.212]) 10, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4RJVWhR003866 10, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 14:31:32 -0500 10, 35 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 10, 35 -- BLUPR04MB548.namprd04.prod.outlook.com (10.141.29.152) with Microsoft SMTP 10, 35 -- Server (TLS) id 15.0.949.11; Tue, 27 May 2014 19:31:18 +0000 10, 35 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.102]) by 10, 35 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.159]) with mapi id 10, 35 -- 15.00.0949.001; Tue, 27 May 2014 19:31:18 +0000 10, 35 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 10, 35 -- To: "nfoster1-at-uncfsu.edu" {nfoster1-at-uncfsu.edu} 10, 35 -- CC: MSA {Microscopy-at-microscopy.com} 10, 35 -- Subject: RE: [Microscopy] Buehler Vibromet polisher help. 10, 35 -- Thread-Topic: [Microscopy] Buehler Vibromet polisher help. 10, 35 -- Thread-Index: AQHPedmqm4yKHAOOrUORjimKJ7xMZZtUyeYG 10, 35 -- Date: Tue, 27 May 2014 19:31:17 +0000 10, 35 -- Message-ID: {1401219072109.20380-at-iastate.edu} 10, 35 -- References: {201405271829.s4RITLKb030628-at-ns.microscopy.com} 10, 35 -- In-Reply-To: {201405271829.s4RITLKb030628-at-ns.microscopy.com} 10, 35 -- Accept-Language: en-US 10, 35 -- Content-Language: en-US 10, 35 -- X-MS-Has-Attach: 10, 35 -- X-MS-TNEF-Correlator: 10, 35 -- x-originating-ip: [129.186.227.3] 10, 35 -- x-forefront-prvs: 02243C58C6 10, 35 -- x-forefront-antispam-report: SFV:NSPM;SFS:(6009001)(428001)(252514010)(377454003)(199002)(189002)(15975445006)(66066001)(79102001)(80022001)(75432001)(76176999)(20776003)(99286001)(83322001)(19580395003)(99396002)(64706001)(50986999)(19580405001)(92726001)(512954002)(92566001)(21056001)(2171001)(54356999)(575784001)(86362001)(87936001)(2656002)(15202345003)(46102001)(36756003)(83072002)(74662001)(101416001)(81342001)(74502001)(31966008)(85852003)(4396001)(81542001)(77982001)(76482001);DIR:OUT;SFP:;SCL:1;SRVR:BLUPR04MB548;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 10, 35 -- received-spf: None (: iastate.edu does not designate permitted sender hosts) 10, 35 -- authentication-results: spf=none (sender IP is ) 10, 35 -- smtp.mailfrom=wesaia-at-iastate.edu; 10, 35 -- Content-Type: text/plain; charset="iso-8859-1" 10, 35 -- MIME-Version: 1.0 10, 35 -- X-OriginatorOrg: iastate.edu 10, 35 -- Content-Transfer-Encoding: 8bit 10, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4RJVWhR003866 ==============================End of - Headers==============================
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Filtered] Rotators for Cold Rooms
Message: Hello to everyone in Microscopy Land. Our old rotator has finally died and we can not get another one like it. We need one that can withstand a 4 degree Celsius cold room and can go end over end like a rotisserie or at least rotate 360 degrees. This seems to be a problem as the bearing grease for most of the ones we bought do not hold up in the cold. Does anyone have any experience with this or knows of one that can withstand these temperatures? We have bought the Rotator Genie and several others but they break down within a couple of days.
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From meds_popular8-at-travelthailand.com Wed May 28 01:16:50 2014 Return-Path: {meds_popular8-at-travelthailand.com} Received: from mail3.travelthailand.com (localhost [113.168.186.81] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4S6GhvH015959 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 28 May 2014 01:16:47 -0500 Message-Id: {201405280616.s4S6GhvH015959-at-ns.microscopy.com}
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Message: UMB Current Electron Microscopy Techniques Workshop
June 2-3, 2014, University of Maryland, Baltimore
Dear Colleagues,
This serves as a reminder that registration for the UMB Current EM Techniques Workshop will close this Friday May 30th, 12:00PM.
This workshop is a two-day event held on June 2nd and 3rd, including oral presentations, live instrument demonstration and hands-on practice with your own samples. The featured instruments include high pressure freezer and freeze substitution systems, a cryo-ultramicrotome, a grid plunger, cryo-TEM and cryo-SEM. A dinner event will be co-sponsored by the Chesapeake Microscopy and Microanalysis Society (CMMS) on June 2nd providing opportunities for social and scientific exchange among workshop participants and CMMS members. Please note that although the Monday morning (June 2nd) presentations featuring keynote speaker, Dr. Peijun Zhang from University of Pittsburg, and application specialists from Leica Microsystems and FEI. Co, are open to the public, registration is still required. Both application talks will be focused on correlative LM-EM (CLEM), a trendy technique in the microscopy field.
More information regarding the workshop and registration can be found on our website http://www.dental.umaryland.edu/Core-imaging/2010web/events/CurrentEMWorkshop.html .
Thank you and we look forward to seeing you in Baltimore.
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==============================Original Headers============================== 23, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed May 28 06:54:18 2014 23, 28 -- Received: from znl.com ([206.69.208.20]) 23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4SBsIuY020540 23, 28 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2014 06:54:18 -0500 23, 28 -- Received: from localhost (localhost [127.0.0.1]) 23, 28 -- by znl.com (Postfix) with ESMTP id 4374970B7FB 23, 28 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2014 06:54:18 -0500 (CDT) 23, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 23, 28 -- Received: from znl.com ([127.0.0.1]) 23, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 23, 28 -- with ESMTP id EIOg6pXSqaiN for {microscopy-at-microscopy.com} ; 23, 28 -- Wed, 28 May 2014 06:54:14 -0500 (CDT) 23, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 23, 28 -- by znl.com (Postfix) with ESMTPA id 5EE9370B7E8 23, 28 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2014 06:54:14 -0500 (CDT) 23, 28 -- Message-ID: {5385CE65.60003-at-microscopy.com} 23, 28 -- Date: Wed, 28 May 2014 06:54:13 -0500 23, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 23, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 23, 28 -- MIME-Version: 1.0 23, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 23, 28 -- Subject: viaWWW:UMB Current Electron Microscopy Techniques Workshop June 2-3 23, 28 -- References: {201405281002.s4SA2QA2018386-at-ns.microscopy.com} 23, 28 -- In-Reply-To: {201405281002.s4SA2QA2018386-at-ns.microscopy.com} 23, 28 -- X-Forwarded-Message-Id: {201405281002.s4SA2QA2018386-at-ns.microscopy.com} 23, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 23, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Historically, I have manually performed the typical two element energy calibration. Afterwards, I can collect objective peak location information which I have logged as evidence of the calibration. I evaluate and record the "as found" condition, perform the energy calibration (if needed), then evaluate and record "as left" information.
Now we have a new system that is totally automated and requires only one element for calibration. It works extremely well as I judge by eye, but I also need unbiased, objective data to document that the calibration "worked", which I cannot access from the system.
We are considering using a certified two element solid solution material as a control sample, but I would like to know what other methods may be typical within the industry. What is everyone else doing for quality assurance with respect to energy calibration?
Thanks,
Chris Holp FENOC BETA Labs
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==============================Original Headers============================== 2, 43 -- From holpc-at-firstenergycorp.com Wed May 28 07:36:03 2014 2, 43 -- Received: from firstenergycorp.com (gw19.firstenergycorp.com [205.132.74.180]) 2, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4SCa3D4007848 2, 43 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2014 07:36:03 -0500 2, 43 -- Received: from WEXCH01P.fenetwork.com (172.17.16.193) by 2, 43 -- WEXCH03P.fenetwork.com (172.17.16.197) with Microsoft SMTP Server (TLS) id 2, 43 -- 15.0.620.29; Wed, 28 May 2014 08:35:54 -0400 2, 43 -- Received: from WXCHGEG01P.fenetwork.com (148.108.128.13) by 2, 43 -- WEXCH01P.fenetwork.com (172.17.16.194) with Microsoft SMTP Server (TLS) id 2, 43 -- 15.0.620.29 via Frontend Transport; Wed, 28 May 2014 08:35:54 -0400 2, 43 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (207.46.163.204) 2, 43 -- by smtp.firstenergycorp.com (148.108.128.13) with Microsoft SMTP Server (TLS) 2, 43 -- id 14.3.123.3; Wed, 28 May 2014 08:35:53 -0400 2, 43 -- Received: from BLUPR05MB149.namprd05.prod.outlook.com (10.255.190.150) by 2, 43 -- BLUPR05MB151.namprd05.prod.outlook.com (10.255.190.148) with Microsoft SMTP 2, 43 -- Server (TLS) id 15.0.949.11; Wed, 28 May 2014 12:35:34 +0000 2, 43 -- Received: from BLUPR05MB149.namprd05.prod.outlook.com ([169.254.9.83]) by 2, 43 -- BLUPR05MB149.namprd05.prod.outlook.com ([169.254.9.83]) with mapi id 2, 43 -- 15.00.0949.001; Wed, 28 May 2014 12:35:33 +0000 2, 43 -- From: "Holp, Christopher R." {holpc-at-firstenergycorp.com} 2, 43 -- To: "Listserver MSA (Microscopy-at-Microscopy.Com)" {Microscopy-at-microscopy.com} 2, 43 -- Subject: EDS and energy calibration 2, 43 -- Thread-Topic: EDS and energy calibration 2, 43 -- Thread-Index: Ac96cUQgzU/arSoDQvyl1MqWrLRt0Q== 2, 43 -- Date: Wed, 28 May 2014 12:35:33 +0000 2, 43 -- Message-ID: {7d2f19d6143341a4bf6c0a3fe72aca57-at-BLUPR05MB149.namprd05.prod.outlook.com} 2, 43 -- Accept-Language: en-US 2, 43 -- Content-Language: en-US 2, 43 -- X-MS-Has-Attach: 2, 43 -- X-MS-TNEF-Correlator: 2, 43 -- x-originating-ip: [205.132.74.4] 2, 43 -- x-forefront-prvs: 0225B0D5BC 2, 43 -- x-forefront-antispam-report: SFV:NSPM;SFS:(6009001)(428001)(164054003)(199002)(189002)(74662001)(83072002)(81542001)(2656002)(50986999)(87936001)(31966008)(74502001)(101416001)(33646001)(92566001)(77982001)(85852003)(81342001)(76576001)(64706001)(4396001)(74316001)(83322001)(21056001)(76482001)(54356999)(99286001)(46102001)(99396002)(20776003)(86362001)(80022001)(79102001)(24736002);DIR:OUT;SFP:;SCL:1;SRVR:BLUPR05MB151;H:BLUPR05MB149.namprd05.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 2, 43 -- received-spf: None (: firstenergycorp.com does not designate permitted sender 2, 43 -- hosts) 2, 43 -- authentication-results: spf=none (sender IP is ) 2, 43 -- smtp.mailfrom=holpc-at-firstenergycorp.com; 2, 43 -- MIME-Version: 1.0 2, 43 -- X-OriginatorOrg: firstenergycorp.com 2, 43 -- Content-Type: text/plain; 2, 43 -- charset="us-ascii" 2, 43 -- Content-Transfer-Encoding: 8bit 2, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4SCa3D4007848 ==============================End of - Headers==============================
In the days before we had ultra thin windows the two element calibration method, usually using aluminium and copper, was almost universal. Now we have thinner windows and we need to have a low energy peak within the new area that has been opened up to us, so using a copper K and L line has now become the norm.
Most of us just accept the data as corrected by the system, but we are able to check for accuracy against other very low energy peaks in our spectrum. That is the simple route now what do others say?
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: holpc-at-firstenergycorp.com [mailto:holpc-at-firstenergycorp.com] Sent: 28 May 2014 13:37 To: protrain-at-emcourses.com
Nick,
When I worked at NASA GRC we used the vibratory polishers for final polishing of microprobe samples. Instead of collodial silica however we used something we called 'Magic Dust'. It is 0.5 micron diamond powder available from Kay Diamond Products. Unlike collodial silica it would keep the samples flat with little contamination. After installing a cloth we would use a diamond extender lubricant from Metlab Corp then sprinkle the Magic Dust on the cloth and put the samples on using the weighted holders for 1 to 2 hours.
No financial interest in Kay or Metlab. Just happy customers for over 20 years.
nfoster1-at-uncfsu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Via Allen Glazner at UNC } } I have resurrected an ancient Buehler Vibromet polisher (model 67-1517) and would like to use it to } put a final polish on probe samples. I have been unable to find cloths or abrasive pads for it (12-inch model) } and wonder if anyone has experience with this polisher. } } Nick } } Nicholas J. Foster } SENCR-MIC Laboratory Technician } Fayetteville State University } Office: (910) 672-2039 } Probe Lab: (910) 672-2037 } Email: nfoster1-at-uncfsu.edu } } ==============================Original Headers============================== } 4, 33 -- From nfoster1-at-uncfsu.edu Tue May 27 13:28:38 2014 } 4, 33 -- Received: from mx0a-000c3a01.pphosted.com (mx0a-000c3a01.pphosted.com [208.84.65.195]) } 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4RISbY0029833 } 4, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 13:28:38 -0500 } 4, 33 -- Received: from pps.filterd (m0001660 [127.0.0.1]) } 4, 33 -- by mx0a-000c3a01.pphosted.com (8.14.5/8.14.5) with SMTP id s4RIRx6x028654 } 4, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 14:28:37 -0400 } 4, 33 -- Received: from exchangeweb.uncfsu.edu ([152.6.0.95]) } 4, 33 -- by mx0a-000c3a01.pphosted.com with ESMTP id 1m4crmaucu-1 } 4, 33 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) } 4, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2014 14:28:37 -0400 } 4, 33 -- Received: from FSUMBX.uncfsu.edu ([fe80::f97c:1637:423b:a461]) by } 4, 33 -- fsumtb1a.uncfsu.edu ([2002:9806:55f::9806:55f]) with mapi; Tue, 27 May 2014 } 4, 33 -- 14:28:35 -0400 } 4, 33 -- From: "Foster, Nicholas" {nfoster1-at-uncfsu.edu} } 4, 33 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 4, 33 -- Date: Tue, 27 May 2014 14:28:35 -0400 } 4, 33 -- Subject: Buehler Vibromet polisher help. } 4, 33 -- Thread-Topic: Buehler Vibromet polisher help. } 4, 33 -- Thread-Index: AQHPedl4CEsLXJ2IQkOjxqHwjE7Ocg== } 4, 33 -- Message-ID: {F2FA8078BCFC0B49AD95B1B3CD75567507A926A9EA-at-FSUMBX.uncfsu.edu} } 4, 33 -- Accept-Language: en-US } 4, 33 -- Content-Language: en-GB } 4, 33 -- X-MS-Has-Attach: } 4, 33 -- X-MS-TNEF-Correlator: } 4, 33 -- acceptlanguage: en-US } 4, 33 -- Content-Type: text/plain; charset="us-ascii" } 4, 33 -- MIME-Version: 1.0 } 4, 33 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 } 4, 33 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 } 4, 33 -- engine=7.0.1-1402240000 definitions=main-1405270250 } 4, 33 -- Content-Transfer-Encoding: 8bit } 4, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4RISbY0029833 } ==============================End of - Headers============================== }
From best_pharmacy15-at-oneidanation.org Wed May 28 11:53:56 2014 Return-Path: {best_pharmacy15-at-oneidanation.org} Received: from smtp2.oneidanation.org (33.50.224.159.triolan.net [159.224.50.33] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4SGrr9I010100 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 28 May 2014 11:53:54 -0500 Message-Id: {201405281653.s4SGrr9I010100-at-ns.microscopy.com}
Greetings! Please join us for our 66th annual microscopy conference held at McCrone Research Institute in Chicago. Inter/Micro is an internationally recognized conference that attracts microscopists from all areas of light and electron microscopy. Research presentations during the first three days cover techniques and instrumentation, environmental and industrial microscopy, and forensic and chemical microscopy. The final two days will be a hands on microscopy workshop.
We look forward to seeing you soon!
Best,
Julie Antia, Inter/Micro Coordinator McCrone Research Institute 312-842-7100
Location McCrone Research Institute 2820 South Michigan Avenue Chicago, IL 60616 www.mcri.org
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==============================Original Headers============================== 9, 26 -- From ph2-at-sprynet.com Wed May 28 17:57:00 2014 9, 26 -- Received: from elasmtp-junco.atl.sa.earthlink.net (elasmtp-junco.atl.sa.earthlink.net [209.86.89.63]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4SMv0iJ009849 9, 26 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2014 17:57:00 -0500 9, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 26 -- s=dk20050327; d=sprynet.com; 9, 26 -- b=nHrYFgxQ5nvzfTaGaMlTOVEMtyJZGqpZmFSkzRFNINC2eq1dUvlCF71nWtTmUR1K; 9, 26 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language:X-ELNK-Trace:X-Originating-IP; 9, 26 -- Received: from [68.21.92.114] (helo=AAHHPdv7) 9, 26 -- by elasmtp-junco.atl.sa.earthlink.net with esmtpa (Exim 4.67) 9, 26 -- (envelope-from {ph2-at-sprynet.com} ) 9, 26 -- id 1Wpmm9-0006Oe-Im; Wed, 28 May 2014 18:56:49 -0400 9, 26 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 9, 26 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 9, 26 -- Subject: FYI - Announcement for Inter/Micro 2014 Conference 9, 26 -- Date: Wed, 28 May 2014 18:56:46 -0400 9, 26 -- Message-ID: {017101cf7ac8$1bc97870$535c6950$-at-sprynet.com} 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Type: text/plain; 9, 26 -- charset="us-ascii" 9, 26 -- Content-Transfer-Encoding: 7bit 9, 26 -- X-Mailer: Microsoft Outlook 14.0 9, 26 -- Thread-Index: Ac96yAEVK1AERZaGT7GhBoRwG8squQ== 9, 26 -- Content-Language: en-us 9, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9d1d1b7ec2aa8069f55996d64062ea580350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 9, 26 -- X-Originating-IP: 68.21.92.114 ==============================End of - Headers==============================
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Email: jlg98-at-cornell.edu Name: john grazul
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Title-Subject: [Filtered] monitor for a JEOL 8900 microprobe
Message: the left monitor on our JXA 8900 microprobe has given up the ghost. Since we are self insured, and really handy, we were wondering if anyone out there has a doner JEOL microprobe or SEM with a monitor that needs a new and loving home. we will pick up the cost of shipping and if you want compensation we can negotiate. If you are in the north east we can even make a road trip, it is pretty desolate up here.
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed May 28 20:23:53 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4T1Nr0o030326 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2014 20:23:53 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 68B9670BF51 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2014 20:23:53 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id aOJhbiVE3XrY for {microscopy-at-microscopy.com} ; 13, 28 -- Wed, 28 May 2014 20:23:48 -0500 (CDT) 13, 28 -- Received: from anlvpn001.nst.anl.gov (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 8839370BF44 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2014 20:23:48 -0500 (CDT) 13, 28 -- Message-ID: {53868C24.7000800-at-microscopy.com} 13, 28 -- Date: Wed, 28 May 2014 20:23:48 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:monitor for a JEOL 8900 microprobe 13, 28 -- References: {201405282051.s4SKpJWH008642-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201405282051.s4SKpJWH008642-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201405282051.s4SKpJWH008642-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are in the process of switching for the first time from a tungsten to a LaB6 emitter on our Tecnai Biotwin 12, circa 2006. I have gotten varying opinions about whether we should shut off the emitter every time we change samples. On our Biotwin (perhaps on all Tecnai 12's?) the gun does not seem to be isolated from the column during specimen exchange (V5 stays open to maintain pumping by the single ion pump), so we are concerned that blowing vacuum during sample exchange (as happens from time to time) could destroy our expensive filament. On the other hand, I have been told that it's not good to turn the LaB6 on and off frequently, as would be necessary to load multiple samples during a session.
I am interested in hearing your experience running a LaB6 on this instrument: do you shut off the beam each time you change samples, and has the emitter been damaged when you have blown vacuum on the column? Also, how long have your emitters been lasting? Thanks!
Marie
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 6, 17 -- From marie.cantino-at-uconn.edu Thu May 29 09:05:29 2014 6, 17 -- Received: from mta3.uits.uconn.edu (mta3.uits.uconn.edu [137.99.203.206]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4TE5T2K027205 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 May 2014 09:05:29 -0500 6, 17 -- Received: from d47h235.public.uconn.edu (d47h235.public.uconn.edu [137.99.47.235]) 6, 17 -- by mta3.uits.uconn.edu (Postfix) with ESMTP id B812066F 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 May 2014 10:05:28 -0400 (EDT) 6, 17 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 6, 17 -- Content-Type: text/plain; charset=us-ascii 6, 17 -- Subject: LaB6 on Tecnai Biotwin 6, 17 -- Date: Thu, 29 May 2014 10:05:28 -0400 6, 17 -- Message-Id: {8A85324C-1D15-44B7-9172-F97FAD74CB23-at-uconn.edu} 6, 17 -- To: Microscopy-at-Microscopy.Com 6, 17 -- Mime-Version: 1.0 (Apple Message framework v1283) 6, 17 -- X-Mailer: Apple Mail (2.1283) 6, 17 -- Content-Transfer-Encoding: 8bit 6, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4TE5T2K027205 ==============================End of - Headers==============================
Dear Dr. Cantino, We have been running a LaB6 emitter in our Philips CM12 for over 20 years. When changing samples we leave the beam saturated. Even the most experienced users and well-trained newer users will, on infrequent occasions, encounter a situation when enough air enters the column after prepumping to cause the high tension to go off and on rare occasions to cause vacuum valves to close as part of the fail/safe system. We believe the impact on the life of the LaB6 emitter is minimal compared to what daily multiple saturation and desaturation would cause.
Typically, our emitters last more than 2000 hours of saturated beam time. Incidentally, it was recommended to us by our service engineer that we leave the high tension on and the beam desaturated at all times when not in use.
Hope this helps, Don
Donald Gantz, MS Dept. Physiology & Biophysics Boston Univ. School of Medicine 700 Albany Street Boston, MA 02118 617-638-4017
On 5/29/2014 10:06 AM, marie.cantino-at-uconn.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We are in the process of switching for the first time from a tungsten to a LaB6 emitter on our Tecnai Biotwin 12, circa 2006. I have gotten varying opinions about whether we should shut off the emitter every time we change samples. On our Biotwin (perhaps on all Tecnai 12's?) the gun does not seem to be isolated from the column during specimen exchange (V5 stays open to maintain pumping by the single ion pump), so we are concerned that blowing vacuum during sample exchange (as happens from time to time) could destroy our expensive filament. On the other hand, I have been told that it's not good to turn the LaB6 on and off frequently, as would be necessary to load multiple samples during a session. } } I am interested in hearing your experience running a LaB6 on this instrument: do you shut off the beam each time you change samples, and has the emitter been damaged when you have blown vacuum on the column? Also, how long have your emitters been lasting? Thanks! } } Marie } } Dr. Marie E. Cantino } Associate Professor of Physiology and Neurobiology } Director, Electron Microscopy Laboratory } University of Connecticut, Unit 3242 } Phone: 860-486-3588 } Fax: 860-486-6369 } } } } ==============================Original Headers============================== } 6, 17 -- From marie.cantino-at-uconn.edu Thu May 29 09:05:29 2014 } 6, 17 -- Received: from mta3.uits.uconn.edu (mta3.uits.uconn.edu [137.99.203.206]) } 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4TE5T2K027205 } 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 May 2014 09:05:29 -0500 } 6, 17 -- Received: from d47h235.public.uconn.edu (d47h235.public.uconn.edu [137.99.47.235]) } 6, 17 -- by mta3.uits.uconn.edu (Postfix) with ESMTP id B812066F } 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 May 2014 10:05:28 -0400 (EDT) } 6, 17 -- From: Marie Cantino {marie.cantino-at-uconn.edu} } 6, 17 -- Content-Type: text/plain; charset=us-ascii } 6, 17 -- Subject: LaB6 on Tecnai Biotwin } 6, 17 -- Date: Thu, 29 May 2014 10:05:28 -0400 } 6, 17 -- Message-Id: {8A85324C-1D15-44B7-9172-F97FAD74CB23-at-uconn.edu} } 6, 17 -- To: Microscopy-at-Microscopy.Com } 6, 17 -- Mime-Version: 1.0 (Apple Message framework v1283) } 6, 17 -- X-Mailer: Apple Mail (2.1283) } 6, 17 -- Content-Transfer-Encoding: 8bit } 6, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s4TE5T2K027205 } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 24 -- From gantz-at-bu.edu Thu May 29 10:38:40 2014 6, 24 -- Received: from relay75.bu.edu (relay75.bu.edu [128.197.228.175]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4TFceFK015170 6, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 29 May 2014 10:38:40 -0500 6, 24 -- X-Envelope-From: gantz-at-bu.edu 6, 24 -- Received: from physbio.bumc.bu.edu (physbio.bumc.bu.edu [155.41.209.20]) 6, 24 -- by relay75.bu.edu (8.14.3/8.13.8) with ESMTP id s4TFcN9t027203; 6, 24 -- Thu, 29 May 2014 11:38:23 -0400 6, 24 -- Received: from [155.41.208.163] (cabr3-dhcp-208-163.bumc.bu.edu [155.41.208.163]) 6, 24 -- (authenticated bits=0) 6, 24 -- by physbio.bumc.bu.edu (8.14.4/8.14.4) with ESMTP id s4TFcGNj000933 6, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO); 6, 24 -- Thu, 29 May 2014 11:38:23 -0400 6, 24 -- Message-ID: {53875469.5080505-at-bu.edu} 6, 24 -- Date: Thu, 29 May 2014 11:38:17 -0400 6, 24 -- From: Don Gantz {gantz-at-bu.edu} 6, 24 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 6, 24 -- MIME-Version: 1.0 6, 24 -- To: marie.cantino-at-uconn.edu, Microscopy-at-microscopy.com 6, 24 -- Subject: Re: [Microscopy] LaB6 on Tecnai Biotwin 6, 24 -- References: {201405291406.s4TE6ewK028246-at-ns.microscopy.com} 6, 24 -- In-Reply-To: {201405291406.s4TE6ewK028246-at-ns.microscopy.com} 6, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We run one or two instrument on LaB6 emitters - a Philips CM30 (300kV) and a JEOL 200CX (200kV).
We always turn the heating current and high tension off for every sample exchange for two reasons. First, there is a substantial danger that you will get a beam of X-rays emerging from the goniometer, especially if the beam is on and the HT is active. We have measured X-ray flux of several hundred micro-Sieverts per hour coming from X-rays emerging from the (empty) goniometer with the beam on, several micro-amps of beam current and the objective aperture left in. With older instruments this is a significant problem (and enough to warrant inspection from the Radiation Safety Officer). If the HT and beam is off, there is no risk at all. Yes, it is a mild inconvenience, but it beats the risk of inadvertently radiating our users.
Second, LaB6 filaments tend to oxidise over time so having a puff of air go up into the gun with a hot filament is unwise. We have about 30-40 users on the instrument and everyone has, at one time, vented the vacuum system during a sample extract. It is a matter of when not if.
The last LaB6 filament we had lasted 3600 hours or about over a year and a half of use.
Yours, Jon
Department of Materials Science & Metallurgy, University of Cambridge, United Kingdom
On 2014-05-29 15:11, marie.cantino-at-uconn.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We are in the process of switching for the first time from a tungsten } to a LaB6 emitter on our Tecnai Biotwin 12, circa 2006. I have gotten } varying opinions about whether we should shut off the emitter every } time we change samples. On our Biotwin (perhaps on all Tecnai 12's?) } the gun does not seem to be isolated from the column during specimen } exchange (V5 stays open to maintain pumping by the single ion pump), } so we are concerned that blowing vacuum during sample exchange (as } happens from time to time) could destroy our expensive filament. On } the other hand, I have been told that it's not good to turn the LaB6 } on and off frequently, as would be necessary to load multiple samples } during a session. } } I am interested in hearing your experience running a LaB6 on this } instrument: do you shut off the beam each time you change samples, and } has the emitter been damaged when you have blown vacuum on the column? } Also, how long have your emitters been lasting? Thanks! } } Marie } } Dr. Marie E. Cantino } Associate Professor of Physiology and Neurobiology } Director, Electron Microscopy Laboratory } University of Connecticut, Unit 3242 } Phone: 860-486-3588 } Fax: 860-486-6369 } } } } ==============================Original } Headers============================== } 6, 17 -- From marie.cantino-at-uconn.edu Thu May 29 09:05:29 2014 } 6, 17 -- Received: from mta3.uits.uconn.edu (mta3.uits.uconn.edu } [137.99.203.206]) } 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s4TE5T2K027205 } 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 May 2014 09:05:29 } -0500 } 6, 17 -- Received: from d47h235.public.uconn.edu } (d47h235.public.uconn.edu [137.99.47.235]) } 6, 17 -- by mta3.uits.uconn.edu (Postfix) with ESMTP id B812066F } 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Thu, 29 May 2014 10:05:28 } -0400 (EDT) } 6, 17 -- From: Marie Cantino {marie.cantino-at-uconn.edu} } 6, 17 -- Content-Type: text/plain; charset=us-ascii } 6, 17 -- Subject: LaB6 on Tecnai Biotwin } 6, 17 -- Date: Thu, 29 May 2014 10:05:28 -0400 } 6, 17 -- Message-Id: {8A85324C-1D15-44B7-9172-F97FAD74CB23-at-uconn.edu} } 6, 17 -- To: Microscopy-at-Microscopy.Com } 6, 17 -- Mime-Version: 1.0 (Apple Message framework v1283) } 6, 17 -- X-Mailer: Apple Mail (2.1283) } 6, 17 -- Content-Transfer-Encoding: 8bit } 6, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s4TE5T2K027205 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 32 -- From jsb43-at-cam.ac.uk Thu May 29 11:19:43 2014 8, 32 -- Received: from ppsw-40.csi.cam.ac.uk (ppsw-40.csi.cam.ac.uk [131.111.8.140]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4TGJhoJ002284 8, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 29 May 2014 11:19:43 -0500 8, 32 -- X-Cam-AntiVirus: no malware found 8, 32 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 8, 32 -- Received: from webmail-2a.csi.cam.ac.uk ([131.111.9.34]:37539) 8, 32 -- by ppsw-40.csi.cam.ac.uk (ppsw.cam.ac.uk [131.111.8.136]:25) 8, 32 -- with esmtp id 1Wq33N-0005ap-k6 (Exim 4.82_3-c0e5623) 8, 32 -- (return-path {jsb43-at-cam.ac.uk} ); Thu, 29 May 2014 17:19:41 +0100 8, 32 -- Received: from [127.0.0.1] (helo=webmail-2.hermes.cam.ac.uk) 8, 32 -- by webmail-2a.csi.cam.ac.uk with esmtp id 1Wq33N-000Mft-7E (Exim 4.80_167-5a66dd3) 8, 32 -- (return-path {jsb43-at-cam.ac.uk} ); Thu, 29 May 2014 17:19:41 +0100 8, 32 -- Received: from host86-132-167-48.range86-132.btcentralplus.com 8, 32 -- ([86.132.167.48]) 8, 32 -- by webmail-2.hermes.cam.ac.uk 8, 32 -- with HTTP (HTTP/1.1 POST); Thu, 29 May 2014 17:19:41 +0100 8, 32 -- MIME-Version: 1.0 8, 32 -- Content-Type: text/plain; charset=UTF-8; 8, 32 -- format=flowed 8, 32 -- Content-Transfer-Encoding: 7bit 8, 32 -- X-Authenticated-User: jsb43 8, 32 -- Date: Thu, 29 May 2014 17:19:41 +0100 8, 32 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 8, 32 -- To: marie.cantino-at-uconn.edu 8, 32 -- Cc: Listserver MSA {Microscopy-at-microscopy.com} 8, 32 -- Subject: Re: [Microscopy] LaB6 on Tecnai Biotwin 8, 32 -- In-Reply-To: {201405291411.s4TEBmBV003203-at-ns.microscopy.com} 8, 32 -- References: {201405291411.s4TEBmBV003203-at-ns.microscopy.com} 8, 32 -- Message-ID: {0c03d47506d48b8fc51bc2ba35294add-at-cam.ac.uk} 8, 32 -- X-Sender: jsb43-at-cam.ac.uk 8, 32 -- User-Agent: Roundcube Webmail/0.9.4 ==============================End of - Headers==============================
From meds-canadian2-at-nationalcablenetworks.ru Thu May 29 11:35:08 2014 Return-Path: {meds-canadian2-at-nationalcablenetworks.ru} Received: from broadband-95-84-206-116.nationalcablenetworks.ru (broadband-95-84-206-116.nationalcablenetworks.ru [95.84.206.116]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4TGZ6bE021549 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 29 May 2014 11:35:06 -0500 Message-Id: {201405291635.s4TGZ6bE021549-at-ns.microscopy.com}
This topic has been discussed a few times, I guess, but I am happy to forward our (overall very positive) experience with LaB6 on FEI 120kV TEM's. Our CM12 is operated with a LaB6 of one manufacturer, at 120kV, always, exclusively, without any problems. Contrast -at-120kV is fine and depends very much on the sample preparation and the specimen quality and ... (also on the voltage, I know, but ...), and also on the quality of the CCD camera and its settings ... Since } 20yrs, we decrease the Filament (= emission) from 18 to 20 down to Zero, during sample exchange, and raising back to 18 to 20, after inserting the holder (and IGP below around 20 or so); that's all. High voltage is left on at 120kV, always, during day time, even when inserting a cryo holder. Really no problems at all, here, on a TEM CM12 used by } 20 users over the years. Our LaB6 filaments (manufacturer on request only) last for } 3 years, the present one for almost four years, with 5 days-a-week-use, 8-12 hours per day. Vacuum on our TEM is really good, with IGP at 5 to 10 (liquid nitrogen is used every day, every hour). We do not open the column ... as we record images fully digitally (no films, since 1998), and the LaB6 stays in the column for 3 to 4 years. This is advantageous ... only positive experience with LaB6 on a TEM of this kind, for bio-samples of all kinds. It also depends on the training of the users, what 'high vacuum' really means, how to achieve it, proper starting of the TEM in the morning, cryo-cycle every night (= IGP off for 4 hours), and so on. kind regards, Reinhard
Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conference: http://www.imc2014.com/ 18th IMC 2014 in Prague, Czech Rep.
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From pharmacy_popular6-at-mgts.by Fri May 30 07:04:47 2014 Return-Path: {pharmacy_popular6-at-mgts.by} Received: from mm-131-197-57-86.dynamic.pppoe.mgts.by ([134.17.143.182]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4UC4iFF025298 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 30 May 2014 07:04:45 -0500 Message-Id: {201405301204.s4UC4iFF025298-at-ns.microscopy.com}
Hello,
I would like to take STEM-EDX maps acquired using Bruker Esprit and open the raw spectral data in Matlab for more hands-on processing of the spectrum images. Has anyone had experience converting the BCF file to another format or importing directly into Matlab? Ideally I would like to convert the product back to BCF as well to make use of what the Esprit software does well.
Thanks! Andrew
==============================Original Headers============================== 3, 35 -- From awagner-at-umn.edu Fri May 30 08:00:39 2014 3, 35 -- Received: from vs-m.tc.umn.edu (vs-m.tc.umn.edu [134.84.135.97]) 3, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4UD0dwa026002 3, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2014 08:00:39 -0500 3, 35 -- Received: from mail-vc0-f170.google.com (mail-vc0-f170.google.com [209.85.220.170]) 3, 35 -- by vs-m.tc.umn.edu (UMN smtpd) with ESMTP 3, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2014 08:00:39 -0500 (CDT) 3, 35 -- X-Umn-Remote-Mta: [N] mail-vc0-f170.google.com [209.85.220.170] #+LO+TS+TR 3, 35 -- X-Umn-Classification: local 3, 35 -- Received: by mail-vc0-f170.google.com with SMTP id la4so2013622vcb.15 3, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2014 06:00:37 -0700 (PDT) 3, 35 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 35 -- d=1e100.net; s=20130820; 3, 35 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to 3, 35 -- :content-type; 3, 35 -- bh=AN4+URBNhm1IuV9UMZNn18jGGyq884BQtmLzmPUuY1c=; 3, 35 -- b=fp+pHH/HlhkXmZWmLzamjht++BeZbiGJ1Ilffx1S+Mmi2wcZwvx3EgOmRb/kKKtChM 3, 35 -- nz7zN8AOqi1LGoAjn8KTfn1yZFLdkyY4711tFNBvVN7HitQ/BnfIDYyignN36Pml38+A 3, 35 -- Md6/waLCedPgv/4RMaS1XPpya2OUIWvl0bOCDoxU5gJrca63XpRYDMPPkQzlJYWRu7No 3, 35 -- koIGaX9+ClprEK6cllTJG5U0iT+7tpl9oTXlHurW9NrLHgtQfxaI5WbgPYssh3l5qr/M 3, 35 -- c5KZn1ynepiWcyIj26BLlfZWL47NyQ8DWmN2SAaYOjeJaO/1w9tfB4/zCld3AWrwCphn 3, 35 -- kPpg== 3, 35 -- X-Gm-Message-State: ALoCoQnz3uCzXw8irT8dblZCwaGsWrzGY6BJQEKMCKPgpXjqoOVfkFKzM/uFp2z4pMUizVNVd7XqeE4E2vnoUwbhH3UxJ55DOM0aMvj+xbBgiMltyULJRh62JZsNnmQ0HUiv7BXvKZcy 3, 35 -- X-Received: by 10.221.4.66 with SMTP id ob2mr13619987vcb.28.1401454837516; 3, 35 -- Fri, 30 May 2014 06:00:37 -0700 (PDT) 3, 35 -- MIME-Version: 1.0 3, 35 -- X-Received: by 10.221.4.66 with SMTP id ob2mr13619972vcb.28.1401454837432; 3, 35 -- Fri, 30 May 2014 06:00:37 -0700 (PDT) 3, 35 -- Received: by 10.220.134.14 with HTTP; Fri, 30 May 2014 06:00:37 -0700 (PDT) 3, 35 -- Date: Fri, 30 May 2014 08:00:37 -0500 3, 35 -- Message-ID: {CAOvwLG4rqMf6s+AccvCGEHn7KAQ6Y-KQoOW8c4VmmhxQGmZzHQ-at-mail.gmail.com} 3, 35 -- Subject: Opening Bruker Esprit EDX HyperMaps in Matlab? 3, 35 -- From: Andrew Wagner {awagner-at-umn.edu} 3, 35 -- To: Microscopy-at-microscopy.com 3, 35 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
I work with an Oxford Aztec that also takes spectral maps. I've also had users ask about taking the data in a portable format that they can work with it on their own. I generally discourage them. At a minimum, I try to discuss with them at length what they have in mind for the data. Many times, it seems that they don't know what they want to do with the data, but they'd like to have it anyway.
Most do not seem to appreciate what would be involved in processing the data. In fact, I had one Chinese student from Civil Engineering ask yesterday if he could get text versions of all his spectra. There are only about 30 spectra, so that isn't too nasty a request. I took one spectrum, exported it in MSA format, imported into Excel, and made a chart. He saw the shape as before but then asked which peaks were which. I showed him where in the MSA file he could find the peak labels and locations including the 4 different peaks for Ca. Then the question came up about how to quantify the data into weight fractions. I did not cover background modeling and removal, nor did I discuss peak deconvolution or integration. I did explain that he would have to have a standard for each of the elements so he could compare his sample intensity to the standard intensity. I did not launch into the necessary matrix corrections. I did tell him that his sample violated many of the assumptions necessary for quant analysis. What I did tell him was enough to dissuade him for following through on his request.
I would go through the same exercise with spectral images. The vendors do a lot in their software and I am hesitant to try to reproduce it. Maybe I am too old and lacking creative juices, but I find it hard to believe that I could seriously improve on what is available. I could see exporting the map images and doing some math on them to try to tease out phases. That would be a much simpler data set to work with.
If you are still set on doing your own processing, I recall NIST was promoting a format for spectral images. They also had developed some routines for basic processes. I have not followed it in recent years, so I cannot tell you where things stand. Maybe someone else can chime in.
Warren Straszheim
-----Original Message----- X-from: awagner-at-umn.edu [mailto:awagner-at-umn.edu] Sent: Friday, May 30, 2014 8:01 AM To: Straszheim, Warren E [BIOTC]
Hello,
I would like to take STEM-EDX maps acquired using Bruker Esprit and open the raw spectral data in Matlab for more hands-on processing of the spectrum images. Has anyone had experience converting the BCF file to another format or importing directly into Matlab? Ideally I would like to convert the product back to BCF as well to make use of what the Esprit software does well.
Thanks! Andrew
==============================Original Headers============================== 3, 35 -- From awagner-at-umn.edu Fri May 30 08:00:39 2014 3, 35 -- Received: from vs-m.tc.umn.edu (vs-m.tc.umn.edu [134.84.135.97]) 3, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s4UD0dwa026002 3, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2014 08:00:39 -0500 3, 35 -- Received: from mail-vc0-f170.google.com (mail-vc0-f170.google.com [209.85.220.170]) 3, 35 -- by vs-m.tc.umn.edu (UMN smtpd) with ESMTP 3, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2014 08:00:39 -0500 (CDT) 3, 35 -- X-Umn-Remote-Mta: [N] mail-vc0-f170.google.com [209.85.220.170] #+LO+TS+TR 3, 35 -- X-Umn-Classification: local 3, 35 -- Received: by mail-vc0-f170.google.com with SMTP id la4so2013622vcb.15 3, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2014 06:00:37 -0700 (PDT) 3, 35 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 35 -- d=1e100.net; s=20130820; 3, 35 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to 3, 35 -- :content-type; 3, 35 -- bh=AN4+URBNhm1IuV9UMZNn18jGGyq884BQtmLzmPUuY1c=; 3, 35 -- b=fp+pHH/HlhkXmZWmLzamjht++BeZbiGJ1Ilffx1S+Mmi2wcZwvx3EgOmRb/kKKtChM 3, 35 -- nz7zN8AOqi1LGoAjn8KTfn1yZFLdkyY4711tFNBvVN7HitQ/BnfIDYyignN36Pml38+A 3, 35 -- Md6/waLCedPgv/4RMaS1XPpya2OUIWvl0bOCDoxU5gJrca63XpRYDMPPkQzlJYWRu7No 3, 35 -- koIGaX9+ClprEK6cllTJG5U0iT+7tpl9oTXlHurW9NrLHgtQfxaI5WbgPYssh3l5qr/M 3, 35 -- c5KZn1ynepiWcyIj26BLlfZWL47NyQ8DWmN2SAaYOjeJaO/1w9tfB4/zCld3AWrwCphn 3, 35 -- kPpg== 3, 35 -- X-Gm-Message-State: ALoCoQnz3uCzXw8irT8dblZCwaGsWrzGY6BJQEKMCKPgpXjqoOVfkFKzM/uFp2z4pMUizVNVd7XqeE4E2vnoUwbhH3UxJ55DOM0aMvj+xbBgiMltyULJRh62JZsNnmQ0HUiv7BXvKZcy 3, 35 -- X-Received: by 10.221.4.66 with SMTP id ob2mr13619987vcb.28.1401454837516; 3, 35 -- Fri, 30 May 2014 06:00:37 -0700 (PDT) 3, 35 -- MIME-Version: 1.0 3, 35 -- X-Received: by 10.221.4.66 with SMTP id ob2mr13619972vcb.28.1401454837432; 3, 35 -- Fri, 30 May 2014 06:00:37 -0700 (PDT) 3, 35 -- Received: by 10.220.134.14 with HTTP; Fri, 30 May 2014 06:00:37 -0700 (PDT) 3, 35 -- Date: Fri, 30 May 2014 08:00:37 -0500 3, 35 -- Message-ID: {CAOvwLG4rqMf6s+AccvCGEHn7KAQ6Y-KQoOW8c4VmmhxQGmZzHQ-at-mail.gmail.com} 3, 35 -- Subject: Opening Bruker Esprit EDX HyperMaps in Matlab? 3, 35 -- From: Andrew Wagner {awagner-at-umn.edu} 3, 35 -- To: Microscopy-at-microscopy.com 3, 35 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
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We are organizing the scientific event: "Materials in Science and Technology 2014 (MIST 2014)". October 13-19, 2014, Oludeniz, Fethiye, Turkey http://mist2014.org
MIST 2014 includes two international conferences and one autumn school: . 2nd International Multidisciplinary Microscopy Congress (INTERM 2014) . 2nd International Congress on Energy Efficiency and Energy Related Materials (ENEFM 2014) . Autumn International School on Growth and Characterization of Advanced Materials (AISMAT 2014)
Specifically, the Autumn School, AISMAT 2014 (http://aismat.org), after a 2.5-day intensive program of lectures, offers its participants the opportunity to attend one of the two conferences. This gives them the option to present their scientific work in any of the two conferences and publish it in its proceedings (Springer Proceedings in Physics). In the school, they will have the twofold advantage of covering both the fundamental principles and current state of the art through lectures from renowned experts from the academic and the industrial sector, as well as the recent trends and achievements through the conference speeches and announcements; and all these, in one of the best resorts in the Turkish Riviera, in the naturally beautiful and culturally rich region of ancient Greek Lykia.
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Bruker have a built in facility to output the data in portable form. In Esprit you can save out data as a "Raw database file" from the File type dropdown menu. This will then save the dataset into a raw binary datacube x,y,energy, that can be imported into Matlab/IDL/whatever... You will need to be familiar with how to import raw binary files. I've done this for Bruker files in the past but of the top of my head I can't remember the order (x,y or energy first?) or the bit depth used, but a bit of playing around should find this out for you (as you already know the data dimensions).
A word of warning however, Bruker save there data in countwise, i.e. for each X-ray counts they place 'energy,x,y' and entry into their datafile. This results in very memory efficient storage. A datacube (such as the "Raw database file") has simply the dimensions of the datacube and as such can be very space inefficient, especially for sparsely sampled data. For example one of my 6Mb Bruker files expanded to 1GB in raw form!
I can't help you with loading the data back in to Esprit after that however (as you cannot import Raw Database files back into the software) this 'one-way' nature is not uncommon as manufacturers formats contains much information, and simulating/re-generating it would be very difficult!
Matthew
On 30/05/2014 11:12 PM, awagner-at-umn.edu wrote: } Hello, } } I would like to take STEM-EDX maps acquired using Bruker Esprit and open } the raw spectral data in Matlab for more hands-on processing of the } spectrum images. Has } anyone had experience converting the BCF file to another format or } importing directly into Matlab? Ideally I would like to convert the product } back to BCF as well to make use of what the Esprit software does well. } } Thanks! } Andrew }
-- Dr M.Weyland, Associate Professor & Titan Manager -------------------------------------------------------------------------- Monash Centre for Electron Microscopy Bldg 81 Monash University Victoria 3800 Australia. -------------------------------------------------------------------------- www.mcem.monash.edu.au ------------------------------------------------------------------------- Ph: (+61) 3 990 59026 --- Fax: (+61) 3 990 53600 --------------------------------------------------------------------------
==============================Original Headers============================== 8, 42 -- From matthew.weyland-at-monash.edu Sun Jun 1 17:52:29 2014 8, 42 -- Received: from na3sys009aog108.obsmtp.com (na3sys009aog108.obsmtp.com [74.125.149.199]) 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s51MqS7R030769 8, 42 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Jun 2014 17:52:29 -0500 8, 42 -- Received: from mail-pb0-f43.google.com ([209.85.160.43]) (using TLSv1) by na3sys009aob108.postini.com ([74.125.148.12]) with SMTP 8, 42 -- ID DSNKU4uurDGNZ2hsxJ3t+9+jsUqYFiPCjOaY-at-postini.com; Sun, 01 Jun 2014 15:52:29 PDT 8, 42 -- Received: by mail-pb0-f43.google.com with SMTP id up15so3567842pbc.16 8, 42 -- for {Microscopy-at-microscopy.com} ; Sun, 01 Jun 2014 15:52:28 -0700 (PDT) 8, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 42 -- d=1e100.net; s=20130820; 8, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 8, 42 -- :cc:subject:references:in-reply-to:content-type 8, 42 -- :content-transfer-encoding; 8, 42 -- bh=GhLTQpM5r8R1NSMu6+bAVhsYs61UVGgb77oJpn1X1nw=; 8, 42 -- b=hSpY9SeuuJnazHWGR0ulaQhCYzRsobIBPdvwggPuQtLJI9ndhS02YmQBAddj2d3hZ5 8, 42 -- oaTQW9Pz2uNTB/0u9mkq4GC9PZFQfUg+CJA7v1ATRowbrHflOwRki5ltT8zfLwEcGYzv 8, 42 -- PTjrGJQZCjQqpd7XCTzcBxcjCcHarmxAfRWGOF7Xoq1QKaOgRJTQ013FFl8CPWk8+Nsu 8, 42 -- yxAFuS2nIUPiZw3JbS2x3rUvM0SpASoNpykqIpoLRef3BpJUdGD30K7nEuoYq0wdQRWO 8, 42 -- Zsz6I5/nCkFhre14d2PIvGzvgn5cYAoW0ovhz7oV1a7Alv10OTwoyUSX2NHgROcuAV36 8, 42 -- H15w== 8, 42 -- X-Gm-Message-State: ALoCoQlHUtqM4LgbEvhCBobwrSKkMv5REuvXVax1+seadvTA881fg56u5LE2IPKraNUjNtoRtJeBacm6a5B8pk1E/x/XQUQF5x4dmwfuVoniE/xxBio6Cp50JQ64/vJVOn+WibzYGNmr 8, 42 -- X-Received: by 10.66.180.141 with SMTP id do13mr35328704pac.93.1401663148248; 8, 42 -- Sun, 01 Jun 2014 15:52:28 -0700 (PDT) 8, 42 -- X-Received: by 10.66.180.141 with SMTP id do13mr35328699pac.93.1401663148133; 8, 42 -- Sun, 01 Jun 2014 15:52:28 -0700 (PDT) 8, 42 -- Received: from [49.127.70.149] (dyn-49-127-70-149.its.monash.edu.au. [49.127.70.149]) 8, 42 -- by mx.google.com with ESMTPSA id cz3sm17143020pbc.9.2014.06.01.15.52.26 8, 42 -- for {multiple recipients} 8, 42 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 8, 42 -- Sun, 01 Jun 2014 15:52:27 -0700 (PDT) 8, 42 -- Message-ID: {538BAEA4.7000908-at-monash.edu} 8, 42 -- Date: Mon, 02 Jun 2014 08:52:20 +1000 8, 42 -- From: Matthew Weyland {matthew.weyland-at-monash.edu} 8, 42 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 8, 42 -- MIME-Version: 1.0 8, 42 -- To: Microscopy-at-microscopy.com 8, 42 -- CC: awagner-at-umn.edu 8, 42 -- Subject: Re: Opening Bruker Esprit EDX HyperMaps in Matlab? 8, 42 -- References: {201405301312.s4UDCUSL010718-at-ns.microscopy.com} 8, 42 -- In-Reply-To: {201405301312.s4UDCUSL010718-at-ns.microscopy.com} 8, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I actually figured it out yesterday with some helpful comments from another listserv member and it's just as you said. The format is Lispix ( http://www.nist.gov/lispix/ and for those who use EDX but might not be aware, see also HyperSpy, http://hyperspy.org/ and DTSA-II, http://www.cstl.nist.gov/div837/837.02/epq/dtsa2/ ). It is a raw binary format with each bit of data in a continuous stream, i.e. no delimiter. Each channel of the first pixel is followed by each channel of the second pixel (left to right, top to bottom). An associated "rpl" file tells you the channel depth per pixel and the height and width of the spectrum image. It is uncalibrated (in space and energy) so that must be taken care of by hand. Bruker opts for 8-bit data, so if you happen to have more than 255 counts in any channel you will have trouble. Esprit appears to truncate the data at the last channel with any counts for sake of file size, so the channel depth is important and potentially variable.
Lispix works great for spectra analysis and moving this data into DTSA. I have not played much with HyperSpy yet. On the off chance anyone else is interested in getting the data into Matlab, feel free to contact me. I put together a matlab script to read the file into a datacube and also perform some basic processing. Simple, but if it saves someone some time in Matlab, great. I am looking at some exceptionally beam sensitive specimens, so I am playing with probe size, step size, dwell time, and probe current to see what compromises I can make while being able to spatially resolve changes in relative composition. That's where Matlab comes in as I'd like to do running sums of the spectra while also doing 2-D running pixel sums as opposed to just binning or increasing probe size or step size.
Some nice discussion of EDX sensitivity, SDD detectors, and approaches to analysis can be found in this powerpoint: http://epmalab.uoregon.edu/Workshop2/DonovanWorkshop07_SDD_Newbury.pdf and this associated paper: http://www.geology.wisc.edu/~johnf/g777/Scanning/NewburyBright-2005.pdf
Cheers, Andrew
On Sun, Jun 1, 2014 at 5:52 PM, Matthew Weyland {matthew.weyland-at-monash.edu} wrote: } Hi Andrew, } } Bruker have a built in facility to output the data in portable form. In } Esprit you can save out data as a "Raw database file" from the File type } dropdown menu. This will then save the dataset into a raw binary datacube } x,y,energy, that can be imported into Matlab/IDL/whatever... You will need } to be familiar with how to import raw binary files. I've done this for } Bruker files in the past but of the top of my head I can't remember the } order (x,y or energy first?) or the bit depth used, but a bit of playing } around should find this out for you (as you already know the data } dimensions). } } A word of warning however, Bruker save there data in countwise, i.e. for } each X-ray counts they place 'energy,x,y' and entry into their datafile. } This results in very memory efficient storage. A datacube (such as the "Raw } database file") has simply the dimensions of the datacube and as such can be } very space inefficient, especially for sparsely sampled data. For example } one of my 6Mb Bruker files expanded to 1GB in raw form! } } I can't help you with loading the data back in to Esprit after that however } (as you cannot import Raw Database files back into the software) this } 'one-way' nature is not uncommon as manufacturers formats contains much } information, and simulating/re-generating it would be very difficult! } } Matthew } } } On 30/05/2014 11:12 PM, awagner-at-umn.edu wrote: } } } } Hello, } } } } I would like to take STEM-EDX maps acquired using Bruker Esprit and open } } the raw spectral data in Matlab for more hands-on processing of the } } spectrum images. Has } } anyone had experience converting the BCF file to another format or } } importing directly into Matlab? Ideally I would like to convert the } } product } } back to BCF as well to make use of what the Esprit software does well. } } } } Thanks! } } Andrew } } } } -- } Dr M.Weyland, Associate Professor & Titan Manager } -------------------------------------------------------------------------- } Monash Centre for Electron Microscopy } Bldg 81 } Monash University } Victoria 3800 } Australia. } -------------------------------------------------------------------------- } www.mcem.monash.edu.au } ------------------------------------------------------------------------- } Ph: (+61) 3 990 59026 --- Fax: (+61) 3 990 53600 } -------------------------------------------------------------------------- }
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realname - Dr. Connie Cummings Email - ultrapathimaging-at-gmail.com ORGANIZATION - DBA UltraPath Imaging EDUCATION - Graduate College LOCATION - Durham, NC USA SUBJECT_OF_QUESTION - EM Speakers/EM lab coaches QUESTION - Good evening all, I am the ultrastructural pathologist who contracts my services to the EM laboratory at NIEHS/NTP, the campus in Research Park, Durham, NC. My question is: We are looking for anyone with EM experience that would be willing to come to NC and spend 2 to 5 days in our EM Lab. Our objective for this visit would be to inspect the lab followed by a list of pertinent items that could be changed. If there are MSA members out there, I would certainly like to hear from you. I hope everyone is having a nice evening. Connie
my phone number 919-316-499 Another contacts: Dr. Herbert's #919-5414613
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Last year, I made a video and an ImageJ plugin for doing this for stacks acquired from the Bruker EDS on the Titan TEM at NCEM. Â
So here is about a 15 minute video where I import the data into ImageJ, make maps, make an RGB, export a spectrum from ImageJ and load it in a spectrum processing software:
https://berkeley.box.com/s/bya3oaiqat25hxwrsate
And here are the links to the source code for the ImageJ plugin which you can use as a starting point for whatever you want to do:
https://berkeley.box.com/s/bd74bnmg405lppfmfakd
and
https://berkeley.box.com/s/brtal2oz4hztj8wfws3y
I hope you find it helpful,
Zack Gainsforth Space Sciences Laboratory, UC Berkeley 7 Gauss Way Berkeley, CA Â 94720 cell: 626-437-9186 zackg-at-ssl.berkeley.edu
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Optimum Embedding Medium for Tissue Cells with Lipid Droplets
Message: Does anyone have an opinion on the optimum embedding medium and its respective physical characteristics (hardness, pH, etc.) for TEM on tissue cells (explants) which contain lipid droplets? We have been using Embed-812 and Durcupan interchangeably. Sometimes our resolution on small droplets is poor and we want to enhance this.
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==============================Original Headers============================== 15, 40 -- From nestor.zaluzec-at-gmail.com Mon Jun 2 15:14:47 2014 15, 40 -- Received: from mail-ig0-f176.google.com (mail-ig0-f176.google.com [209.85.213.176]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s52KEkSK023772 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jun 2014 15:14:47 -0500 15, 40 -- Received: by mail-ig0-f176.google.com with SMTP id hl10so3836481igb.15 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 02 Jun 2014 13:14:46 -0700 (PDT) 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 40 -- d=gmail.com; s=20120113; 15, 40 -- h=sender:from:message-id:date:reply-to:user-agent:mime-version:to 15, 40 -- :subject:references:in-reply-to:content-type 15, 40 -- :content-transfer-encoding; 15, 40 -- bh=sYzayMr96vMoKZRmLJUMQdzMHDpmbNSbzzZxerB4JpU=; 15, 40 -- b=uwTicApcXdpVVRLAK2z5TnZwwY8xU2XH7G+n6a5kbEDKdgd61pl/2gpdAX8or6uGNY 15, 40 -- pDeGm8V3+HGL+uvJDq149kmeBs0ZRw5VqyWoLJv/Fz+gbUH4IO2OfKPohgVqGWJskxWo 15, 40 -- H4CBf0TZSaqjl/NIkYt9smQ9/UhabcWn40jwMooocK33+zM8inunJjjkefJLiLvcfF1W 15, 40 -- zq8JTUvhoyR7GgcBvVHYwmXxzZhMwco0crfba7/8/agGOJo7GucgpvDfw9USMp9EsET+ 15, 40 -- wRHYLED0L1SlI0VYgsJqjAFAN0v14+le6K0nVkiFXZh1BIeipOs+MNvtSPfKsU068KjT 15, 40 -- n5qA== 15, 40 -- X-Received: by 10.50.20.8 with SMTP id j8mr22287703ige.17.1401740086715; 15, 40 -- Mon, 02 Jun 2014 13:14:46 -0700 (PDT) 15, 40 -- Received: from Nestors-MacBook-Pro-2014.local (PC-Hudak1-J.imr.McMaster.CA. [130.113.54.34]) 15, 40 -- by mx.google.com with ESMTPSA id fk7sm31168090igb.9.2014.06.02.13.14.45 15, 40 -- for {microscopy-at-microscopy.com} 15, 40 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 15, 40 -- Mon, 02 Jun 2014 13:14:45 -0700 (PDT) 15, 40 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 15, 40 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 40 -- Message-ID: {538CDB38.3020705-at-microscopy.com} 15, 40 -- Date: Mon, 02 Jun 2014 16:14:48 -0400 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 15, 40 -- MIME-Version: 1.0 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 40 -- Subject: viaWWW:Optimum Embedding Medium for Tissue Cells 15, 40 -- References: {201406021949.s52JnfN2023418-at-ns.microscopy.com} 15, 40 -- In-Reply-To: {201406021949.s52JnfN2023418-at-ns.microscopy.com} 15, 40 -- X-Forwarded-Message-Id: {201406021949.s52JnfN2023418-at-ns.microscopy.com} 15, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From cheapest-medications11-at-caviarbazaar.com Tue Jun 3 02:29:29 2014 Return-Path: {cheapest-medications11-at-caviarbazaar.com} Received: from smtp3.caviarbazaar.com ([217.23.68.10]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s537TPE4016270 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 3 Jun 2014 02:29:26 -0500 Message-Id: {201406030729.s537TPE4016270-at-ns.microscopy.com}
Hi all
As I'm trying to make reworking some "old" optical microscope, I come with a few questions to solve one problem.
I've a set of Zeiss Epiplan OL from the 70" (designed for the Standard et Universal generation), but coming from the metallographic microscope mounted on a Siemens Microprobe. They were factory modified to be mounted in vacuum on a special turret while the microscope body was fixed on the column on the air side. The body of the objectives is shorter than the standard one. As I want to use them on a Photomicroscope, I need to machine extension tubes with 2 RMS threads.
My questions are about the parfocal length of these OL. -First, does someone know if these objectives did exist in 33 and 45 mm parfocal length, or only in 45 mm ? -Secondly, how could I determine this parfocal length, which I need to know the length for the extension tubes.
As I know a working distance for each OL (from a Zeiss doc), I can calculate two possible extension lengths. I tried some tests on a optical bench, fixing a 160 mm tube length and trying to determine the length between the thread plane of that virtual tube and the back of the lens, but as the image depth become very long with high power obectives, it is very difficult to locate the best focus position. Could it be possible to see something reliable looking at distortion and chromatic aberation near the border of the field ?
All other idees are wellcome ! Thanks
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] operating manual
Message: Hi
My friend is looking for operating manuals for FEI TEM T-20 and HRTEM F-30
Best wishes Rashmi
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] Spares for EM
Message: HI
We are looking for the procedure to buy the spares directly from FEI for ESEM, TEM and HRTEM
best wishes Rashmi
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Optimum Embedding Medium for Tissue Cells with Lipid Droplets
Message: Does anyone have an opinion on the optimum embedding medium and its respective physical characteristics (hardness, pH, etc.) for TEM on tissue cells (explants) which contain lipid droplets? We have been using Embed-812 and Durcupan interchangeably. Sometimes our resolution on small droplets is poor and we want to enhance this.
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==============================Original Headers============================== 4, 36 -- From bfoster-at-the-mip.com Tue Jun 3 09:32:00 2014 4, 36 -- Received: from nm11-vm8.access.bullet.mail.bf1.yahoo.com (nm11-vm8.access.bullet.mail.bf1.yahoo.com [216.109.114.231]) 4, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s53EW0DU032495 4, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Jun 2014 09:32:00 -0500 4, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1401805920; bh=TFbt8/JUkC01j/cXPzwr+ea13Lg1moEGLB2nnUrumeg=; h=Received:Received:Received:X-Yahoo-Newman-Id:Message-ID:X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:X-Mailer:Date:To:From:Subject:Mime-Version:Content-Type; b=qmat5KwlcUzXL3kOvrXo8NeLSSQgs7D3g1yO/W7Sr/XzIlOYeweJK0uJKiw1+t0o0u7nbfS1rwVmNwUTHAkfzQb3s0QDXX4ukGEfshOlXu+K71pzGRA8EGReQ1u73KVlQ4jRutsbIp/wsuNinmTjGY8x6CZtpRCui6cGpknOW3cHFgib1OYDpYu4HDfWz8WkwZ3UtT71ME64FVOalbiXyRkcZ9R3zVAL4cc2D0ddwA2xnBDiTFtPVXegHmHarSnK3ZlBchDqaVf1RXR4zZ1RrC8PA2G//6wr/bi0NcGeX5acZBAXrCuVBmZrnPX/qtWGv5Tiv+gsh32/vzGaBPAaog== 4, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s2048; d=yahoo.com; 4, 36 -- b=O+0NEurqFN8ecA+9ZFC5+j8qlpm3ZGQO4yNWNqoY/crxl3L/49BTAVFuZaBR+DRqXCDT6SpfIYdlU1my4PjsN4YiilExFstqYuyoFIFyMqFYwhoLgrtlRP4KdUcR/FW2WLY64cg1WI0MRuMP8ljY0b97oNVQhqDo3jNADJj60OP2hOEWFrNrwEDraVaOFU2YT1ff+hy2eCC8cuCz6vLK9DtAaRLS4pdOpPmOeDDzVMBTe7apIeIcHxXJTjGLAuLbUkRmgNG3KWgNCTH/rirKSdr/EOSJZGtMYInoxyZeCEtHw7XYbWBnysiX0No20Haq1a12hFbc8bclWrxm33eN6Q==; 4, 36 -- Received: from [66.196.81.163] by nm11.access.bullet.mail.bf1.yahoo.com with NNFMP; 03 Jun 2014 14:31:59 -0000 4, 36 -- Received: from [98.138.226.242] by tm9.access.bullet.mail.bf1.yahoo.com with NNFMP; 03 Jun 2014 14:31:59 -0000 4, 36 -- Received: from [127.0.0.1] by smtp113.sbc.mail.ne1.yahoo.com with NNFMP; 03 Jun 2014 14:31:59 -0000 4, 36 -- X-Yahoo-Newman-Id: 875431.74433.bm-at-smtp113.sbc.mail.ne1.yahoo.com 4, 36 -- Message-ID: {875431.74433.bm-at-smtp113.sbc.mail.ne1.yahoo.com} 4, 36 -- X-Yahoo-Newman-Property: ymail-3 4, 36 -- X-YMail-OSG: OLmzL3wVM1ljjZ6fkp62hlTIIUrakk1m.c3FOaZVGIXLajj 4, 36 -- iLIVX3NVsQsxDsJREVMLtH6bgLu25O6zgR6vuAn4o050iPn.ud588XSYYRP8 4, 36 -- I9W44yAkE5YPbRFiCMo7AFknFfLuzPV.qxu1wQ3xD7f_D1XblCEiGxQMinPZ 4, 36 -- IbFSewvBp.LRDUxLGbUqh2ZNwaVtbCjxCvy65TD10_4m.sSZMdRWgt9KYYGB 4, 36 -- Sb3l_MfCysuX7DrMQ5e9AwKlMz9RXpxuDwL7mkRmBOTNJhVqdHCqEQE3QmHW 4, 36 -- RFlrbnpybQEZzJWR2xug7URcVwmuOhMMeetJLCcCfiKBIhgDlqiULbS9maMw 4, 36 -- I5PkslGBAy5UsmHXB5dNbXhtmZAFgGWbtL9tmB1SNOpBtfobnsdy_KIrKNiT 4, 36 -- mU8oXMcGC7LbHG5YM_SY7ZJ.TFM3dHtvuVqXrtc6aYuL7cyZ2J2C9KkewvAe 4, 36 -- nR00FKbWf1VtWOYDvPDGaVDMA8W_TSnPMGuGfxfP2Gl9CodX0jqk0stQkcQ1 4, 36 -- xTctw3IQLdH7Lh9fE9M789xLpKyt5Ir7jnM.UwYyUaE2snlkzY9w5ZlY7.bq 4, 36 -- YQSM3Lzxam6mfhPAZy4Xo4n318V7ARnQomu7uKnysBzL2qR9uQEHkQZDle3A 4, 36 -- l5J0YVBXeI30wJvg6Nsn2NxDty7CgOxHirCyuer7d3bpNjA-- 4, 36 -- X-Yahoo-SMTP: KOmzyESswBATZkB2214rZDIfSSNffJ2urJX_LO8NW63iukNVtw-- 4, 36 -- X-Rocket-Received: from Bs-PC.the-mip.com (bfoster-at-99.168.105.118 with plain [98.138.84.52]) 4, 36 -- by smtp113.sbc.mail.ne1.yahoo.com with SMTP; 03 Jun 2014 14:31:59 +0000 UTC 4, 36 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 4, 36 -- Date: Tue, 03 Jun 2014 09:31:56 -0500 4, 36 -- To: Microscopy-at-microscopy.com 4, 36 -- From: Barbara Foster {bfoster-at-the-mip.com} 4, 36 -- Subject: Webinar: How image integrity impacts your ability to 4, 36 -- =?iso-8859-1?Q?publish=85?= or perish. 4, 36 -- Mime-Version: 1.0 4, 36 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
The problem is that these objectives have a modified body which is much shorter then normaly. That's why I don't know for which parfocal distance they were originaly build. The extention tubes I want to machine will in fact replace the missing body. Following the Universal/phomi documentation from Zeiss, it seems that both 33 and 45 mm parfocal distances epicondenser/objectives have been provided. I don't know if my explanation is clear ! A picture would be simpler.
==============================Original Headers============================== 9, 26 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Jun 3 10:15:03 2014 9, 26 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s53FF3Gc019700 9, 26 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jun 2014 10:15:03 -0500 9, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id 0845A242384; 9, 26 -- Tue, 3 Jun 2014 17:14:51 +0200 (CEST) 9, 26 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr 9, 26 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) 9, 26 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) 9, 26 -- with ESMTP id 81drar159fBq; Tue, 3 Jun 2014 17:14:46 +0200 (CEST) 9, 26 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 9, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id 9497B2A200E; 9, 26 -- Tue, 3 Jun 2014 17:14:46 +0200 (CEST) 9, 26 -- Message-ID: {538DE668.2080209-at-ipcms.u-strasbg.fr} 9, 26 -- Date: Tue, 03 Jun 2014 17:14:48 +0200 9, 26 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 9, 26 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:17.0) Gecko/20130330 Thunderbird/17.0.5 9, 26 -- MIME-Version: 1.0 9, 26 -- To: John Mitchels {john.mitchels-at-gmail.com} , microscopy-at-microscopy.com 9, 26 -- Subject: Re: [Microscopy] OM : how to determine the parfocal length of an 9, 26 -- OL 9, 26 -- References: {201406030929.s539TsNq025528-at-ns.microscopy.com} {CAHX7yTReHwkeTMwTAU6-6SaaRdZOYdFAhcqycbpZSZagyj3aHw-at-mail.gmail.com} 9, 26 -- In-Reply-To: {CAHX7yTReHwkeTMwTAU6-6SaaRdZOYdFAhcqycbpZSZagyj3aHw-at-mail.gmail.com} 9, 26 -- Content-Type: text/plain; charset=UTF-8; format=flowed 9, 26 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From reasonable-medications2-at-iservco.com Tue Jun 3 19:52:17 2014 Return-Path: {reasonable-medications2-at-iservco.com} Received: from smtp1.iservco.com (94.19.170.115.pool.sknt.ru [94.19.170.115] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s540qF3l013187 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 3 Jun 2014 19:52:16 -0500 Message-Id: {201406040052.s540qF3l013187-at-ns.microscopy.com}
X-from: zhenquan.liu-at-asu.edu ()
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Email: zhenquan.liu-at-asu.edu Name: Zhenquan Liu
Organization: Arizona State University
Title-Subject: [Filtered] SEM for Cornea
Message: Some one is asking me to do SEM on his samples of cornea. I do SEM on metals, semiconductors and so on normally. I do not have much experiences on this kind of biological samples.
If some one on this list server knows how to do it, please tell me.
And I have a SEM of Fei XL-30 available for this sample.
Thanks in advance.
Zhenquan Liu
Arizona State University
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--
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
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Title-Subject: [Filtered] SEM - LaB6 filament users
Message: Dear microscopists,
my questions are for SEM - LaB6 filament users.
When you do quantitative analysis or long run analysis do you use the second saturation point or you leave it at the first point (cross shape)?
Do you turn off the filament heat (Fil. I=0A) when you are not using your SEM or you turn it to a stand by mode (Fil. I. lower)?
What is the LaB6 filament life time when you use it at the second point of saturation?
Thank you very much for your help!
Valerie Lecomte
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
the preparation of biological material for SEM depends very much of the instruments you have at your disposal. Unlike metals and semiconductors, biological material contains a lot of water and, because you have to remove it for SEM, the material loses its structure and tends to be very unstable under high vacuum. For this reason the biologists need critical point dryers for example. Hopefully you have access to such instruments as well as to the person with the expertise to use it.
By googling with "preparation cornea for EM", I found the first link to be interesting: http://www.optics.rochester.edu/workgroups/cml/opt307/spr05/anant/
best regards, Stephane
-------------------------------------------- On Wed, 6/4/14, zaluzec-at-microscopy.com {zaluzec-at-microscopy.com} wrote:
Subject: [Microscopy] viaWWW:SEM for Cornea To: nizets2-at-yahoo.com Date: Wednesday, June 4, 2014, 1:00 PM
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Email: zhenquan.liu-at-asu.edu Name: Zhenquan Liu
Organization: Arizona State University
Title-Subject: [Filtered] SEM for Cornea
Message: Some one is asking me to do SEM on his samples of cornea. I do SEM on metals, semiconductors and so on normally. I do not have much experiences on this kind of biological samples.
If some one on this list server knows how to do it, please tell me.
And I have a SEM of Fei XL-30 available for this sample.
Thanks in advance.
Zhenquan Liu
Arizona State University
Login Host: 149.169.145.20 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
--
---===[|]===---
=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
I have a user on FIB620 FIB/SEM with old pre-Xp dual-beam control software (Ver. 6.3 from FEI/Phillips), who needs to find out how many dwell points would be made by ion beam on 1um long line.
The calculation would be straight forward if we knew overlap (available in material file or on the screen) and the beam diameter assumed by the software, but... the assumed beam diameter is hidden somewhere.
Obviously such information can be derived by application of good old hacking and some investment of time, but I was wondering if there is anyone out there who already knows where does software store beam diameter parameters.
Thank you very much beforehand,
-- Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
==============================Original Headers============================== 7, 37 -- From vray-at-partbeamsystech.com Wed Jun 4 11:24:18 2014 7, 37 -- Received: from nm20.bullet.mail.bf1.yahoo.com (nm20.bullet.mail.bf1.yahoo.com [98.139.212.179]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s54GOIDW015578 7, 37 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 11:24:18 -0500 7, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1401899058; bh=fpw69jdyGS1f6zai1uS/93d/xvqzXrmyCyCORpIQZ7o=; h=Received:Received:Received:X-Yahoo-Newman-Id:X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:X-Rocket-Received:Message-ID:Date:From:Reply-To:User-Agent:MIME-Version:To:Subject:Content-Type:Content-Transfer-Encoding; b=n2wCPC7nIh28HSKi+49SZ1IFIqsKgtIZk0M7KT+xxEJNiWVCDoYv701TPKpzy9rcsBEhoR111FwO/6kh5mLjmHESg69SW9u00M6SibTXYuesDJ8M9LhMnJaazvQcTuWTyYoDgULqnoU4KFDUHnVw92ceP82EgmdggE72w8rplmYRdr16pNr5gv5QyBHLHD/RzGrCWwH6EebngCljfdQfPol7b7wXaJ2f+3L8tHWE0KKyAg1LCt72FYLmA9s1Bx45UrhAA6Ysj1BDkSAxtuPRCJi4bMwE662oIyldnfDEXN/jmVL4paLUXV43lCP9AeRPug3ecHRMj++ET6eLzWr1Qg== 7, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s2048; d=yahoo.com; 7, 37 -- b=si9QyI6yMVWhhnRGcseXoGdGgxVFuPsvUmpavbn1j/OjR/fE53FRJNIxIE1kXGZjGNuZS11W7YGaFPMdBGw628iyoeeZxmKzq9PE7BYUEBai83cb2K+xlacf5iA0+/FQlTeFvG/3lVVSahls06S6rc8rTPdnyNnFq8gHUoNwJqpUeR/Jod0DCIi/p/oSnV/HcETdTT4HiZqIhRC0jzRS/TQhMTNlEbuZ1TJWwtYcGv6XrX0iDP2jqEgU5iw7CDTKA4KAUajaO5qgd1luDaUQXOET/YF0b+51Y3qqK7yDXk3x7W2jVI3WjztPZ1ZBxJ8Foy1sE7yE0r1ArV7K34eS6A==; 7, 37 -- Received: from [66.196.81.173] by nm20.bullet.mail.bf1.yahoo.com with NNFMP; 04 Jun 2014 16:24:18 -0000 7, 37 -- Received: from [68.142.230.72] by tm19.bullet.mail.bf1.yahoo.com with NNFMP; 04 Jun 2014 16:24:18 -0000 7, 37 -- Received: from [127.0.0.1] by smtp229.mail.bf1.yahoo.com with NNFMP; 04 Jun 2014 16:24:18 -0000 7, 37 -- X-Yahoo-Newman-Id: 64624.10078.bm-at-smtp229.mail.bf1.yahoo.com 7, 37 -- X-Yahoo-Newman-Property: ymail-3 7, 37 -- X-YMail-OSG: BQItjJQVM1kTIBzEHZVoYhH0o.Adxy0IBWhIoRXTzi7uQg9 7, 37 -- XiDLUk9SkaW.1VdAsYzqmHVWC_6Qcj7JOgxmm5IpcWjRm_Jb2Ne63I0GAQ2M 7, 37 -- iMWYYsWYt6UxfYdgqLbm4jcu1wSS4Ru.MghAXR5lh9QSFvF4v_nodhpKGmiU 7, 37 -- U3bQNqSHUxSRxQHQcqy0ie4sTzwDMfRheYR.S.K_S0x8X2BqqXrerYI86lzs 7, 37 -- 7.teNpQJUGBhtjzfZ09UibMcGyQ18UR9mygiTqKKuV.bi9wG9YFCmgP2XMee 7, 37 -- yqm7ZqevPT4RqEsY0Ntjra9gn9zw_FgCzrPZIC9ByybSY0LCMQhpoci0cFST 7, 37 -- jYnYM_ppSCnj48Qw6O4pqXOAydPy_LueGyY7JQpQn_M.RtYMIXB11f9S9hBD 7, 37 -- 2pxlQlCcpnmnOGbYEHtDQe2BIYubVi6H3kmZ8g64z3dLcs2lfG4PeaUdhrgn 7, 37 -- Pbfd3.1XoY2YUANM7qYT4TPhpqIA1cfytfPCbeo4je1V85QDN.moi0RcPkrB 7, 37 -- xGsO_Q1LZR3VfYr22KXI9jmeDzNP.LJUzz_Ys6ZPBLUtOUsPgVtmPMopuU3C 7, 37 -- sEsR8oFgmYF6glEka09M3WFkh3Rxuw0ss_zOoCdG_Q62hAqWvAbMH40LHEJ8 7, 37 -- qwnqwAcY0Jb_L5tBcOkRIwVuJgqIP5i2nHYa7Cg-- 7, 37 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 37 -- X-Rocket-Received: from [10.109.166.54] (vray-at-129.2.129.153 with plain [98.139.211.125]) 7, 37 -- by smtp229.mail.bf1.yahoo.com with SMTP; 04 Jun 2014 16:24:18 +0000 UTC 7, 37 -- Message-ID: {538F4832.6090706-at-partbeamsystech.com} 7, 37 -- Date: Wed, 04 Jun 2014 12:24:18 -0400 7, 37 -- From: Valery Ray {vray-at-partbeamsystech.com} 7, 37 -- Reply-To: vray-at-partbeamsystech.com 7, 37 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 7, 37 -- MIME-Version: 1.0 7, 37 -- To: microscopy-at-microscopy.com 7, 37 -- Subject: Raster definition parameters for old FIB 620 FIB/SEM from FEI/Phillips 7, 37 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 37 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am trying to correlate super resolution imaging with TEM on Tokuyasu frozen sections. As an internal marker in fluorescence, we would like to stain the the nuclei before cryoprotection/freezing. I would appreciate any recommendations for a rather robust vital nuclear stain. The STED imaging is done in green channel.
Thanks, Michal --
Michal Jarnik, Ph.D.
Electron Microscope Facility
Cell Biology and Metabolism Program
National Institute of Child Health and Human Development National Institutes of Health
==============================Original Headers============================== 9, 30 -- From michal.jarnik-at-nih.gov Wed Jun 4 11:30:04 2014 9, 30 -- Received: from nihxway5out.hub.nih.gov (nihxway5out.hub.nih.gov [128.231.90.113]) 9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s54GU3E0021617 9, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 11:30:03 -0500 9, 30 -- X-IronPortListener: Outbound_SMTP 9, 30 -- X-IronPort-Anti-Spam-Filtered: true 9, 30 -- X-IronPort-Anti-Spam-Result: AgAFABtJj1OcKEes/2dsb2JhbABZgweBKsQBFnSCLBIoUQEVKS8TJwQ1iCCdCIR/sBQXkxkEj3eKHIcZjCCDOIIv 9, 30 -- X-IPAS-Result: AgAFABtJj1OcKEes/2dsb2JhbABZgweBKsQBFnSCLBIoUQEVKS8TJwQ1iCCdCIR/sBQXkxkEj3eKHIcZjCCDOIIv 9, 30 -- Received: from unknown (HELO CASHTV08.nih.gov) ([156.40.71.172]) 9, 30 -- by nihxway5out.hub.nih.gov with ESMTP/TLS/AES128-SHA; 04 Jun 2014 12:29:51 -0400 9, 30 -- Received: from MLBXV14.nih.gov ([169.254.4.90]) by CASHTV08.nih.gov 9, 30 -- ([156.40.71.172]) with mapi id 14.03.0174.001; Wed, 4 Jun 2014 12:29:50 -0400 9, 30 -- From: "Jarnik, Michal (NIH/NICHD) [E]" {michal.jarnik-at-nih.gov} 9, 30 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 9, 30 -- Subject: Vital DNA stain 9, 30 -- Thread-Topic: Vital DNA stain 9, 30 -- Thread-Index: AQHPgBI04OW4u6INdUCCz5k0i4JjHw== 9, 30 -- Date: Wed, 4 Jun 2014 16:29:50 +0000 9, 30 -- Message-ID: {CFB4C1BD.2AE0%jarnikm-at-mail.nih.gov} 9, 30 -- Accept-Language: en-US 9, 30 -- Content-Language: en-US 9, 30 -- X-MS-Has-Attach: 9, 30 -- X-MS-TNEF-Correlator: 9, 30 -- user-agent: Microsoft-MacOutlook/14.0.0.100825 9, 30 -- x-originating-ip: [156.40.71.190] 9, 30 -- Content-Type: text/plain; charset="us-ascii" 9, 30 -- Content-ID: {496F0B31F181FB4E994B495770020BEB-at-mail.nih.gov} 9, 30 -- MIME-Version: 1.0 9, 30 -- Content-Transfer-Encoding: 8bit 9, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s54GU3E0021617 ==============================End of - Headers==============================
From cheapest_drugs9-at-rr.com Wed Jun 4 11:36:41 2014 Return-Path: {cheapest_drugs9-at-rr.com} Received: from cpe-184-153-142-200.maine.res.rr.com (cpe-184-153-142-200.maine.res.rr.com [184.153.142.200]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s54Gaeor005926 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 4 Jun 2014 11:36:40 -0500 Message-Id: {201406041636.s54Gaeor005926-at-ns.microscopy.com}
Have you tried DRAQ5? You could excite the dye with a 488 nm laser - Is that what you mean "green channel"?
Zhaojie Zhang University of Wyoming
-----Original Message----- X-from: michal.jarnik-at-nih.gov [mailto:michal.jarnik-at-nih.gov] Sent: Wednesday, June 04, 2014 10:33 AM To: Z.J. Zhang
I am trying to correlate super resolution imaging with TEM on Tokuyasu frozen sections. As an internal marker in fluorescence, we would like to stain the the nuclei before cryoprotection/freezing. I would appreciate any recommendations for a rather robust vital nuclear stain. The STED imaging is done in green channel.
Thanks, Michal --
Michal Jarnik, Ph.D.
Electron Microscope Facility
Cell Biology and Metabolism Program
National Institute of Child Health and Human Development National Institutes of Health
DRAQ5 is far red, so 488 excitation would be far from optimal. What about SYBR Green?
Jill Pflugheber St. Lawrence Universtiy
-----Original Message----- X-from: ZZhang-at-uwyo.edu [mailto:ZZhang-at-uwyo.edu] Sent: Wednesday, June 04, 2014 12:54 PM To: Jill Pflugheber
Have you tried DRAQ5? You could excite the dye with a 488 nm laser - Is that what you mean "green channel"?
Zhaojie Zhang University of Wyoming
-----Original Message----- X-from: michal.jarnik-at-nih.gov [mailto:michal.jarnik-at-nih.gov] Sent: Wednesday, June 04, 2014 10:33 AM To: Z.J. Zhang
I am trying to correlate super resolution imaging with TEM on Tokuyasu frozen sections. As an internal marker in fluorescence, we would like to stain the the nuclei before cryoprotection/freezing. I would appreciate any recommendations for a rather robust vital nuclear stain. The STED imaging is done in green channel.
Thanks, Michal --
Michal Jarnik, Ph.D.
Electron Microscope Facility
Cell Biology and Metabolism Program
National Institute of Child Health and Human Development National Institutes of Health
I have scanned in all the old images that I thought were good and now want to get rid of the TEM and SEM negatives I have had for many years. Does anyone know if they are recyclable?
Thanks JoAnn Buchanan Stanford University School of Medicine
==============================Original Headers============================== 3, 34 -- From buchsmith-at-gmail.com Wed Jun 4 18:50:11 2014 3, 34 -- Received: from mail-pb0-f50.google.com (mail-pb0-f50.google.com [209.85.160.50]) 3, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s54NoAkm001206 3, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 18:50:11 -0500 3, 34 -- Received: by mail-pb0-f50.google.com with SMTP id ma3so214039pbc.23 3, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Jun 2014 16:50:10 -0700 (PDT) 3, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 34 -- d=gmail.com; s=20120113; 3, 34 -- h=from:content-type:content-transfer-encoding:subject:message-id:date 3, 34 -- :to:mime-version; 3, 34 -- bh=t2n+1K3g19IoijK89e+Zi5i87+UjX87kMj/nlGqZG+c=; 3, 34 -- b=TjxTRzK6Vw9XhNumf2zt7tW6O5b3nYUV62XGe8ZY9GRuyddpILbxd+Wk9YIvizpUfo 3, 34 -- TRgRY0sqAgVqVgc29ku8lvSsxkrw1BQxNso8gmdOcI22ZU0S61jVp7QsecnFNwDoA9Fq 3, 34 -- ssyvkb1a0buV7T+LnHbsybmsdoD8FjoR4cPsos9ko6IWVArTmFP+NvrSqBRak21XgloL 3, 34 -- RTVpK/NhKPrRzzSlCUXeR2QPNqNkjFwLZmDKCCT9HR0PQQ0npy6rQGwR0Ppb/HbE1SSd 3, 34 -- rBFz3BJQyzQ7cFNlBy44Bmneaw2MEAP0JHjvitHDKgKEnveKlkkZLfQEIUED+xidw1AD 3, 34 -- ahiA== 3, 34 -- X-Received: by 10.68.178.131 with SMTP id cy3mr70423530pbc.146.1401925810033; 3, 34 -- Wed, 04 Jun 2014 16:50:10 -0700 (PDT) 3, 34 -- Received: from dn52emiu.sunet (DN2lk5ms0.Stanford.EDU. [171.66.219.128]) 3, 34 -- by mx.google.com with ESMTPSA id nh8sm15058381pbc.25.2014.06.04.16.50.08 3, 34 -- for {Microscopy-at-microscopy.com} 3, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 3, 34 -- Wed, 04 Jun 2014 16:50:08 -0700 (PDT) 3, 34 -- From: JoAnn Buchanan {buchsmith-at-gmail.com} 3, 34 -- Content-Type: text/plain; charset=us-ascii 3, 34 -- Subject: Disposing of old TEM negatives 3, 34 -- Message-Id: {F8EE2D9A-BB9F-41AC-8A1D-180FC2574495-at-gmail.com} 3, 34 -- Date: Wed, 4 Jun 2014 16:50:07 -0700 3, 34 -- To: Microscopy-at-microscopy.com 3, 34 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.2\)) 3, 34 -- X-Mailer: Apple Mail (2.1878.2) 3, 34 -- Content-Transfer-Encoding: 8bit 3, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s54NoAkm001206 ==============================End of - Headers==============================
Yes, your TEM and SEM negatives are recyclable. Rochester Silver Works (http://www.rochestersilverworks.com/) does this. For many years they were part of Eastman Kodak and they recycled over 50 years of negatives from our lab. I suspect that there are other recyclers who do as well.
Note: I have no financial interest in Rochester Silver Works. I simply used them to be environmentally responsible.
Best regards, John Minter Kodak Analytical Sciences
==============================Original Headers============================== 4, 37 -- From jrminter-at-gmail.com Wed Jun 4 19:16:58 2014 4, 37 -- Received: from mail-ie0-f181.google.com (mail-ie0-f181.google.com [209.85.223.181]) 4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s550GwUO020593 4, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 19:16:58 -0500 4, 37 -- Received: by mail-ie0-f181.google.com with SMTP id rp18so227753iec.26 4, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Jun 2014 17:16:58 -0700 (PDT) 4, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 37 -- d=gmail.com; s=20120113; 4, 37 -- h=content-type:mime-version:subject:from:in-reply-to:date:cc 4, 37 -- :content-transfer-encoding:message-id:references:to; 4, 37 -- bh=paOhc0zqYWYV4TWntzPyl1kt5tXRGPzD4YJAdqVjRH8=; 4, 37 -- b=eXSCM9L4E2sKWilGtDL6kEYCb6SMfXs7oIvNjDLvISh3ceKxsgOzmDzxWae5oYPQ4l 4, 37 -- wfmpE/yqAW0+bIPUKOUdvrByxFMPVZdPYVBsGa6NZCc9oz0mjQe1A+uAY3/ytSE/xliK 4, 37 -- SrlPL6lkmLbcc75EGR2Rp6MwyAuQIN6xjcddAiauEDHok2xR6l+w6tMp20rHCCsJKB7Y 4, 37 -- UA0elAy9Pd0g0hLWKXUYrP3KVx4E/69yYtIp6gbReOXgvScMOY8VNes1MBvW+/VmP3dw 4, 37 -- MEro7/7CfZzGWxfHn6xa9b36GMKUTgzi4/U/+ouANIYD5O91RegairrkB9mmJ2j/5Lbp 4, 37 -- hnFQ== 4, 37 -- X-Received: by 10.50.254.132 with SMTP id ai4mr13254078igd.30.1401927418354; 4, 37 -- Wed, 04 Jun 2014 17:16:58 -0700 (PDT) 4, 37 -- Received: from [10.0.1.25] (cpe-66-66-193-190.rochester.res.rr.com. [66.66.193.190]) 4, 37 -- by mx.google.com with ESMTPSA id g2sm48832114igc.12.2014.06.04.17.16.57 4, 37 -- for {multiple recipients} 4, 37 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 4, 37 -- Wed, 04 Jun 2014 17:16:57 -0700 (PDT) 4, 37 -- Content-Type: text/plain; charset=us-ascii 4, 37 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.2\)) 4, 37 -- Subject: Re: [Microscopy] Disposing of old TEM negatives 4, 37 -- From: John Minter {jrminter-at-gmail.com} 4, 37 -- In-Reply-To: {201406050002.s5502scs017443-at-ns.microscopy.com} 4, 37 -- Date: Wed, 4 Jun 2014 20:17:08 -0400 4, 37 -- Cc: Microscopy-at-microscopy.com 4, 37 -- Message-Id: {F3B47E98-FC45-4B5E-9F0E-A75EFD478EF8-at-gmail.com} 4, 37 -- References: {201406050002.s5502scs017443-at-ns.microscopy.com} 4, 37 -- To: buchsmith-at-gmail.com 4, 37 -- X-Mailer: Apple Mail (2.1878.2) 4, 37 -- Content-Transfer-Encoding: 8bit 4, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s550GwUO020593 ==============================End of - Headers==============================
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Email: sbras-at-uw.edu Name: Scott Braswell
Organization: University of Washington
Title-Subject: [Filtered] In-situ NP measurement
Message: Hi,
I received an email yesterday asking me to characterize the size of FeOx nanoparticles in strong NaHO. I wonder if drying the solution would result in too much salt. My thoughts are to either try cryoTEM or use a liquid-cell TEM holder. Any insight you can offer for this sample prep would be appreciated.
Thanks, Scott
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Email: zhenquan.liu-at-asu.edu Name: Zhenquan Liu
Organization: Arizona State University
Title-Subject: [Filtered] Wet method on Evironmental SEM for cornea
Message: Hi, Everyone,
Thanks a lot for replying from several people for my previous email which asking for a way to do SEM on cornea. I have learned a lot form these replies. There are so many things I have to go through sample preparation and actually we do not have most of the instruments in our lab, but there are available in School of Biology.
I read these replies carefully and I did not see anyone who mentioned wet method on environmental SEM to get images from cornea. We have Fei XL-30 Environmental SEM. We can do wet method.
A wet method is to introduce some water vapor near the sample to keep sample wet. The humidity can be controlled to 100%.
I think that it might be difficult to do so. On this list server there are many experts in biology field, but none of them mentioned wet method. I think if it is a good way to work on cornea, people would have told me. Many years ago I tried wet method on the surface of the samples in water. I did not get much useful information. I might miss some thing when I did it.
Therefore if someone has some experiences on wet method, even better on cornea, please tell me. It will be great.
Thanks to Stephane, Lee Cohen-Gould, Debra Sherman and Ed Haller and more people who replied to my previous email.
Zhenquan Liu Arizona State University
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We have an FEI Quanta and could probably work in the same regime as you can. Also like you, I am a materials scientist, not a biologist, but I get the occasional question to look at biological material. Most recently, it was an aggregate of bacteria. The request came from a Civil/Environmental Engineer so I don't think they understood what they were asking nor would they quite know how to interpret what I found, if I had found something. That should be the first question - What are they looking for and what do they expect it to look like?
A lot of biological material will not be that interesting given all the water present and the organic membranes. In my little experience, the unprepared samples just don't have much contrast when you are looking at the outside. It is cell wall and maybe a film of water. I would be interested in hearing what the biologists have to say about imparting contrast. Of course, the structure should be much more interesting on the inside but I suppose it needs enhancement, even then.
I understand it is technically possible to keep a sample from dehydrating. The triple point of water is at 0.006 atm or 600 Pa at near 0C, so it should be possible to maintain a saturated atmosphere. Of course, that requires a cooling stage. If you are working at room temperature, that number rises to about 2300 Pa. That presents more of a challenge. I was not able to keep my bacteria granule from drying out in the SEM.
I'll be interested in hearing any recipes that prove helpful.
Warren Straszheim Iowa State University
-----Original Message----- X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] Sent: Wednesday, June 04, 2014 8:18 PM To: Straszheim, Warren E [BIOTC]
Organization: Arizona State University
Title-Subject: [Filtered] Wet method on Evironmental SEM for cornea
Message: Hi, Everyone,
Thanks a lot for replying from several people for my previous email which asking for a way to do SEM on cornea. I have learned a lot form these replies. There are so many things I have to go through sample preparation and actually we do not have most of the instruments in our lab, but there are available in School of Biology.
I read these replies carefully and I did not see anyone who mentioned wet method on environmental SEM to get images from cornea. We have Fei XL-30 Environmental SEM. We can do wet method.
A wet method is to introduce some water vapor near the sample to keep sample wet. The humidity can be controlled to 100%.
I think that it might be difficult to do so. On this list server there are many experts in biology field, but none of them mentioned wet method. I think if it is a good way to work on cornea, people would have told me. Many years ago I tried wet method on the surface of the samples in water. I did not get much useful information. I might miss some thing when I did it.
Therefore if someone has some experiences on wet method, even better on cornea, please tell me. It will be great.
Thanks to Stephane, Lee Cohen-Gould, Debra Sherman and Ed Haller and more people who replied to my previous email.
Zhenquan Liu Arizona State University
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==============================Original Headers============================== 20, 40 -- From nestor.zaluzec-at-gmail.com Wed Jun 4 20:17:14 2014 20, 40 -- Received: from mail-ie0-f174.google.com (mail-ie0-f174.google.com [209.85.223.174]) 20, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s551HCmB010778 20, 40 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 20:17:13 -0500 20, 40 -- Received: by mail-ie0-f174.google.com with SMTP id lx4so294962iec.19 20, 40 -- for {microscopy-at-microscopy.com} ; Wed, 04 Jun 2014 18:17:12 -0700 (PDT) 20, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 20, 40 -- d=gmail.com; s=20120113; 20, 40 -- h=sender:from:message-id:date:reply-to:user-agent:mime-version:to 20, 40 -- :subject:references:in-reply-to:content-type 20, 40 -- :content-transfer-encoding; 20, 40 -- bh=eN8YnlyxqL5Smw9dr/nFsrVEmY2gz0woIzu6r+VxKWc=; 20, 40 -- b=tUYpcif2jknEg/ubFyty+MZEoz2vg6FTmtukmJzcM7rSyZ9+mRDUnU0Thxiyy9bDFB 20, 40 -- lb1dS7+MSIq+soAixK8zVtNt+LIpMoEaGBE0BFx+0MYh0do6YhP6dZ+uHVc6pRW4VMTM 20, 40 -- m9GTgWIQCnfubvGIt1ibK6C11xHtWClA+YELSwTIvrhMKjyK1Iph2Vw4A0dHglCTIQiC 20, 40 -- v8PPudg/ztHjFmr7hmj9aXB3LI1hG3LZYiZOyb8LokRycOQSrOM6wgShptwc2ELI8SXf 20, 40 -- BmFGEUXtbWSPV+EjxPPC1LO82HxAICkHHAev15UzSjWav1R8epMRMQpHHrnWSd0UnyDv 20, 40 -- 4TvA== 20, 40 -- X-Received: by 10.50.28.51 with SMTP id y19mr13591917igg.5.1401931031781; 20, 40 -- Wed, 04 Jun 2014 18:17:11 -0700 (PDT) 20, 40 -- Received: from Nestors-MacBook-Pro-2014.local (d221-87-97.commercial.cgocable.net. [216.221.87.97]) 20, 40 -- by mx.google.com with ESMTPSA id e4sm20685627igx.4.2014.06.04.18.16.43 20, 40 -- for {microscopy-at-microscopy.com} 20, 40 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 20, 40 -- Wed, 04 Jun 2014 18:17:11 -0700 (PDT) 20, 40 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 20, 40 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 20, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 40 -- Message-ID: {538FC4FA.90708-at-microscopy.com} 20, 40 -- Date: Wed, 04 Jun 2014 21:16:42 -0400 20, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 20, 40 -- MIME-Version: 1.0 20, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 40 -- Subject: viaWWW:Wet method on Evironmental SEM for cornea 20, 40 -- References: {201406041628.s54GSUEF019344-at-ns.microscopy.com} 20, 40 -- In-Reply-To: {201406041628.s54GSUEF019344-at-ns.microscopy.com} 20, 40 -- X-Forwarded-Message-Id: {201406041628.s54GSUEF019344-at-ns.microscopy.com} 20, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 20, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 25, 34 -- From wesaia-at-iastate.edu Wed Jun 4 22:12:04 2014 25, 34 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2lp0242.outbound.protection.outlook.com [207.46.163.242]) 25, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s553C3YB014799 25, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 22:12:03 -0500 25, 34 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 25, 34 -- BLUPR04MB547.namprd04.prod.outlook.com (10.141.29.145) with Microsoft SMTP 25, 34 -- Server (TLS) id 15.0.954.9; Thu, 5 Jun 2014 03:12:02 +0000 25, 34 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.68]) by 25, 34 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.119]) with mapi id 25, 34 -- 15.00.0954.000; Thu, 5 Jun 2014 03:12:01 +0000 25, 34 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 25, 34 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 25, 34 -- CC: "zhenquan.liu-at-asu.edu" {zhenquan.liu-at-asu.edu} 25, 34 -- Subject: RE: [Microscopy] viaWWW:Wet method on Environmental SEM for cornea 25, 34 -- Thread-Topic: [Microscopy] viaWWW:Wet method on Environmental SEM for cornea 25, 34 -- Thread-Index: Ac+Aa8BgM7B6I821S7ipVoJ4GI7rXw== 25, 34 -- Date: Thu, 5 Jun 2014 03:12:00 +0000 25, 34 -- Message-ID: {8cfca567d56946b297eb71f99561f949-at-BLUPR04MB546.namprd04.prod.outlook.com} 25, 34 -- Accept-Language: en-US 25, 34 -- Content-Language: en-US 25, 34 -- X-MS-Has-Attach: 25, 34 -- X-MS-TNEF-Correlator: 25, 34 -- x-originating-ip: [69.66.102.132] 25, 34 -- x-microsoft-antispam: BL:0;ACTION:Default;RISK:Low;SCL:0;SPMLVL:NotSpam;PCL:0;RULEID: 25, 34 -- x-forefront-prvs: 0233768B38 25, 34 -- x-forefront-antispam-report: SFV:NSPM;SFS:(6009001)(428001)(377454003)(199002)(189002)(51444003)(252514010)(64544002)(13464003)(33646001)(74502001)(15975445006)(15202345003)(19580395003)(54356999)(86362001)(31966008)(92566001)(101416001)(83322001)(50986999)(21056001)(2656002)(81342001)(575784001)(74662001)(74316001)(19580405001)(77982001)(76576001)(85852003)(79102001)(83072002)(87936001)(81542001)(75432001)(76482001)(20776003)(4396001)(80022001)(66066001)(88552001)(46102001)(24736002);DIR:OUT;SFP:;SCL:1;SRVR:BLUPR04MB547;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 25, 34 -- received-spf: None (: iastate.edu does not designate permitted sender hosts) 25, 34 -- authentication-results: spf=none (sender IP is ) 25, 34 -- smtp.mailfrom=wesaia-at-iastate.edu; 25, 34 -- Content-Type: text/plain; charset="us-ascii" 25, 34 -- MIME-Version: 1.0 25, 34 -- X-OriginatorOrg: iastate.edu 25, 34 -- Content-Transfer-Encoding: 8bit 25, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s553C3YB014799 ==============================End of - Headers==============================
I'm helping to dismantle an old EM lab on short notice. Mostly I took pictures of stuff that others on this campus (University of Hawaii at Manoa) might want, but I thought I'd see if anyone here needs any parts. Small parts. I'm willing to grab and ship smaller, easy-to-pack items only. I can be bribed with chocolate. I'm too old and cranky to ship whole electron microscopes anymore.
You can see some pictures at https://www.hawaii.edu/filedrop/dl/hLsCi-hIrgZ-WRbVA-WRCIZ/
There's a Zeiss DSM962 regular tungsten SEM, plugged in and pumping all this time, but probably not used since 2009-ish. Spare parts kit and manuals. The newest part on it is probably the penning gauge. I'm willing to grab that sort of thing (the penning gauge).
It has an Oxford Link EDS system. Probably useless now.
There's a BioRad PT7100 RF Plasma Barrel Etcher that I know nothing about.
And there's a Hummer II sputter coater that I used to own and was sorry I gave to them long ago, but probably doesn't work. I didn't check for targets, but I can.
Slim pickings, but I promised them I'd see if anyone wanted anything before it all has to go, like by Tuesday!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 11, 20 -- From tina-at-pbrc.hawaii.edu Wed Jun 4 22:23:41 2014 11, 20 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s553NfH7030261 11, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 22:23:41 -0500 11, 20 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 11, 20 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id s553NehA021936 11, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 11, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 17:23:40 -1000 (HST) 11, 20 -- Received: from localhost (tina-at-localhost) 11, 20 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id s553NdDP021932 11, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 17:23:40 -1000 (HST) 11, 20 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 11, 20 -- Date: Wed, 4 Jun 2014 17:23:39 -1000 (HST) 11, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 11, 20 -- X-X-Sender: tina-at-sf-v240 11, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 11, 20 -- Subject: Taking apart SEM lab 11, 20 -- Message-ID: {Pine.GSO.4.64.1406041713280.20758-at-sf-v240} 11, 20 -- MIME-Version: 1.0 11, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
From popular_pharmacy2-at-hinet.net Thu Jun 5 00:38:44 2014 Return-Path: {popular_pharmacy2-at-hinet.net} Received: from 223-142-168-115.dynamic.hinet.net (223-142-168-115.dynamic.hinet.net [223.142.168.115]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s555cV7I023340 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 5 Jun 2014 00:38:35 -0500 Message-Id: {201406050538.s555cV7I023340-at-ns.microscopy.com}
Dear Scott,
the most important point in my view is to know if the presence of NaOH is critical for the maintenance of the structure of the NP. If not then it is not such a big problem. NaOH can be eliminate by ultracentrifugation or filtration, provided that the filter pore size is adequate. A note of caution however: if I am right iron is ferromagnetic and the NP may be attracted by the strong lenses of the TEM. I would be more concerned by that.
Regards, Stephane
-------------------------------------------- On Thu, 6/5/14, zaluzec-at-microscopy.com {zaluzec-at-microscopy.com} wrote:
Subject: [Microscopy] viaWWW:In-situ NP measurement To: nizets2-at-yahoo.com Date: Thursday, June 5, 2014, 3:19 AM
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Email: sbras-at-uw.edu Name: Scott Braswell
Organization: University of Washington
Title-Subject: [Filtered] In-situ NP measurement
Message: Hi,
I received an email yesterday asking me to characterize the size of FeOx nanoparticles in strong NaHO. I wonder if drying the solution would result in too much salt. My thoughts are to either try cryoTEM or use a liquid-cell TEM holder. Any insight you can offer for this sample prep would be appreciated.
Thanks, Scott
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Fluorescent dyes which stain nucleic acids are intercalating agents, don't expect them to be "vital" (well it depends on how long you want to observe the living cells). Vital stains also have to permeate live cells and even permeate them 2x for the nucleus, one through the cell membrane and one for the nuclear envelope, which is not so easy. I don't mean to be deceptive but as far as I know all nuclear labels are based on nucleic acid stain, meaning they are not vital. That said, it is seldom necessary to stain the nucleus both in LM and EM in single cell experiments because it is such a big structure with high contrast. I hope someone proves me wrote and offers you a solution.
regards, Stephane
-------------------------------------------- On Wed, 6/4/14, jpflugheber-at-stlawu.edu {jpflugheber-at-stlawu.edu} wrote:
Subject: [Microscopy] Vital DNA stain To: nizets2-at-yahoo.com Date: Wednesday, June 4, 2014, 7:34 PM
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DRAQ5 is far red, so 488 excitation would be far from optimal. What about SYBR Green?
Jill Pflugheber St. Lawrence Universtiy
-----Original Message----- X-from: ZZhang-at-uwyo.edu [mailto:ZZhang-at-uwyo.edu] Sent: Wednesday, June 04, 2014 12:54 PM To: Jill Pflugheber Subject: [Microscopy] RE: Vital DNA stain
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Have you tried DRAQ5? You could excite the dye with a 488 nm laser - Is that what you mean "green channel"?
Zhaojie Zhang University of Wyoming
-----Original Message----- X-from: michal.jarnik-at-nih.gov [mailto:michal.jarnik-at-nih.gov] Sent: Wednesday, June 04, 2014 10:33 AM To: Z.J. Zhang Subject: [Microscopy] Vital DNA stain
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I am trying to correlate super resolution imaging with TEM on Tokuyasu frozen sections. As an internal marker in fluorescence, we would like to stain the the nuclei before cryoprotection/freezing. I would appreciate any recommendations for a rather robust vital nuclear stain. The STED imaging is done in green channel.
Thanks, Michal --
Michal Jarnik, Ph.D.
Electron Microscope Facility
Cell Biology and Metabolism Program
National Institute of Child Health and Human Development National Institutes of Health
==============================Original Headers============================== 26, 30 -- From jpflugheber-at-stlawu.edu Wed Jun 4 12:30:44 2014 26, 30 -- Received: from mx3.stlawu.edu (mx3.stlawu.edu [69.6.96.28]) 26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s54HUiOn009637 26, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 12:30:44 -0500 26, 30 -- Received: from mail.stlawu.edu (ex-hubcas-01.stlawu.local [10.1.10.14]) 26, 30 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 26, 30 -- (No client certificate requested) 26, 30 -- by mx3.stlawu.edu (Postfix) with ESMTP id 3A7E31012FB 26, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jun 2014 13:30:44 -0400 (EDT) 26, 30 -- Received: from EX-MAILBOX-01.stlawu.local ([fe80::d8e5:9bc3:cc08:12c3]) by 26, 30 -- ex-hubcas-01.stlawu.local ([::1]) with mapi id 14.03.0174.001; Wed, 4 Jun 26, 30 -- 2014 13:30:44 -0400 26, 30 -- From: Jill Pflugheber {jpflugheber-at-stlawu.edu} 26, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 26, 30 -- Subject: RE: [Microscopy] RE: Vital DNA stain 26, 30 -- Thread-Topic: [Microscopy] RE: Vital DNA stain 26, 30 -- Thread-Index: AQHPgBWKdC7Iz0mqT0SrTM2pbEHb6pthNJLA 26, 30 -- Date: Wed, 4 Jun 2014 17:30:43 +0000 26, 30 -- Message-ID: {1088303B25EC2F4987A6D330992AB1A6ED13FC25-at-ex-mailbox-01.stlawu.local} 26, 30 -- References: {201406041653.s54Gra1Z032553-at-ns.microscopy.com} 26, 30 -- In-Reply-To: {201406041653.s54Gra1Z032553-at-ns.microscopy.com} 26, 30 -- Accept-Language: en-US 26, 30 -- Content-Language: en-US 26, 30 -- X-MS-Has-Attach: 26, 30 -- X-MS-TNEF-Correlator: 26, 30 -- x-originating-ip: [10.60.5.24] 26, 30 -- Content-Type: text/plain; charset="us-ascii" 26, 30 -- MIME-Version: 1.0 26, 30 -- Content-Transfer-Encoding: 8bit 26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s54HUiOn009637 ==============================End of - Headers==============================
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From medications_canadian5-at-twinmagicians.com Thu Jun 5 07:45:32 2014 Return-Path: {medications_canadian5-at-twinmagicians.com} Received: from smtp4.twinmagicians.com ([94.245.156.45]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s55CjTa1002495 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 5 Jun 2014 07:45:30 -0500 Message-Id: {201406051245.s55CjTa1002495-at-ns.microscopy.com}
I have worked with XL30 ESEM-FEG and with Quantas. For biological samples there are some realities often poorly understood until you starting trying things yourself:
1) You can't really see much on samples that are truly "wet". You will see a flat boring film of water. 2) There is a point during the pumping process, before the ESEM valve starts cycling, where you actually have no control over the chamber environment. Some dehydration WILL occur during this stage. 3) If samples are in solution, any dehydration will cause salts to precipitate and obscure what you want to see.
Assuming you have the cooling stage, these problems can be (mostly) worked around. Placing a few drops of water around the sample, on the Peltier stage, can help keep the local environment "more humid" during pumpdown. Pre-chilling the sample helps for the same reason. Using too much water can lead to other problems, such as rapid bubbling which can dislodge a loose sample or splatter on the final lens/detector/etc. Keep a long working distance during pumpdown.
As Warren mentioned, make sure you really understand what they/you are looking for. Growth or debris on the surface? These should be possible. Shape distortions or internal structure? Maybe not so much.
I did actually look at corneas and other eye parts some years ago. The truth is that we got the best results, given what the customer was looking for, with a 'gentle' fixative followed by a few rinses and then imaging in ESEM. Sorry that I can't remember the fix or solution, a med student came up with those. You are still getting some advantage by not coating the samples, and you have several minutes to an hour or more in which to study the surface...if your humidity control is good. This is certainly not to say that ESEM doesn't work, it's great for many things but you will have trouble with the most delicate, high moisture content samples. In reality you are trying to keep the humidity as close to 100% as you can, and work reasonably quickly. Once water droplets actually form on your sample, imaging is usually poor. Make small adjustments with ESEM pressure and stage temperature, wait for the system to stabilize a bit and go again.
Also note that the Peltier sample holders cannot be too large. We had one made to keep the entire cornea or lens in contact with cold metal. Even then, the top surface may be wetter OR dryer than the rest.
Make sure you watch some samples drying out so that you really understand what the initial dehydration artifacts will look like. Good luck!
Matt Lynn Forensic Analytical
On Wed, Jun 4, 2014 at 8:25 PM, {wesaia-at-iastate.edu} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have an FEI Quanta and could probably work in the same regime as you can. Also like you, I am a materials scientist, not a biologist, but I get the occasional question to look at biological material. Most recently, it was an aggregate of bacteria. The request came from a Civil/Environmental Engineer so I don't think they understood what they were asking nor would they quite know how to interpret what I found, if I had found something. That should be the first question - What are they looking for and what do they expect it to look like? } } A lot of biological material will not be that interesting given all the water present and the organic membranes. In my little experience, the unprepared samples just don't have much contrast when you are looking at the outside. It is cell wall and maybe a film of water. I would be interested in hearing what the biologists have to say about imparting contrast. Of course, the structure should be much more interesting on the inside but I suppose it needs enhancement, even then. } } I understand it is technically possible to keep a sample from dehydrating. The triple point of water is at 0.006 atm or 600 Pa at near 0C, so it should be possible to maintain a saturated atmosphere. Of course, that requires a cooling stage. If you are working at room temperature, that number rises to about 2300 Pa. That presents more of a challenge. I was not able to keep my bacteria granule from drying out in the SEM. } } I'll be interested in hearing any recipes that prove helpful. } } Warren Straszheim } Iowa State University } } -----Original Message----- } X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] } Sent: Wednesday, June 04, 2014 8:18 PM } To: Straszheim, Warren E [BIOTC] } Subject: [Microscopy] viaWWW:Wet method on Evironmental SEM for cornea } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } X-from: zhenquan.liu-at-asu.edu () } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both zhenquan.liu-at-asu.edu as well as the Microscopy } Listserver } --------------------------------------------------------------------------- } Email: zhenquan.liu-at-asu.edu } Name: Zhenquan Liu } } Organization: Arizona State University } } Title-Subject: [Filtered] Wet method on Evironmental SEM for cornea } } Message: Hi, Everyone, } } Thanks a lot for replying from several people for my previous email which asking for a way to do SEM on cornea. I have learned a lot form these replies. There are so many things I have to go through sample preparation and actually we do not have most of the instruments in our lab, but there are available in School of Biology. } } I read these replies carefully and I did not see anyone who mentioned wet method on environmental SEM to get images from cornea. We have Fei } XL-30 Environmental SEM. We can do wet method. } } A wet method is to introduce some water vapor near the sample to keep sample wet. The humidity can be controlled to 100%. } } I think that it might be difficult to do so. On this list server there are many experts in biology field, but none of them mentioned wet method. I think if it is a good way to work on cornea, people would have told me. Many years ago I tried wet method on the surface of the samples in water. I did not get much useful information. I might miss some thing when I did it. } } Therefore if someone has some experiences on wet method, even better on cornea, please tell me. It will be great. } } Thanks to Stephane, Lee Cohen-Gould, Debra Sherman and Ed Haller and more people who replied to my previous email. } } Zhenquan Liu } Arizona State University } } } }
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Email: mlibbee-at-lbl.gov Name: Marissa
Organization: NCEM, LBNL
Title-Subject: [Filtered] annealing nanoparticles on c-film grids
Message: Has anyone annealed nanoparticles on TEM c-film grids? If so, can you tell me the temp and time at which the carbon started to break down? And the specific grid you used for the experiment?
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I wonder if there are experts in working with an Edwards 306 Turbo Evaporator.
We have had this machine working with no trouble at all for many years but had to move it.
Once moved we re-plumbed the water cooling system and wired it up following recommended instructions.
Now it will pump down but we cannot get power to the electrodes. Following the instruction book we have reset all the circuit breakers we could find but to no avail. The small LEDs do nto even light up on the control panel next to the LT/HT switch.
Ideally it would be great to have someone we could contact in Southern California (Pasadena area) who could take a look at the machine for us - and get it working.
Failing that, is there anyone who could offer some useful tips for us to try so that we can get this great machine working again.
Sincerely,
Paul Webster.
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
I have been heating C-films on gold 200 mesh grids up to 1000°C in-situ in the electron diffraction camera without damage for the film during my work on the lowering of the melting temperature of gold nanoparticles (P. Buffat, Phys Rev A13 (1976) 2287). The oven was designed to create a black body environment and ensure a nanoparticle temperature measurement as accurate as possible.
Trials with Mo grids failed because either the grid roughness forbids any film gliding under thermal expansion or a chemical reaction occurs between Mo and C that could lead to Mo-carbide.
Kind regards
Philippe Buffat
==============================Original Headers============================== 6, 19 -- From philippe.buffat-at-epfl.ch Fri Jun 6 05:35:04 2014 6, 19 -- Received: from smtp4.epfl.ch (smtp4.epfl.ch [128.178.224.219]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s56AZ3hP009548 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jun 2014 05:35:03 -0500 6, 19 -- Received: (qmail 19142 invoked by uid 107); 6 Jun 2014 10:35:02 -0000 6, 19 -- X-Virus-Scanned: ClamAV 6, 19 -- Received: from cimepc22.epfl.ch (HELO [128.178.98.64]) (128.178.98.64) 6, 19 -- by mail.epfl.ch (AngelmatoPhylax SMTP proxy) with ESMTP; Fri, 06 Jun 2014 12:35:02 +0200 6, 19 -- Message-ID: {53919958.4080506-at-epfl.ch} 6, 19 -- Date: Fri, 06 Jun 2014 12:35:04 +0200 6, 19 -- From: Philippe Buffat {philippe.buffat-at-epfl.ch} 6, 19 -- Reply-To: philippe.buffat-at-epfl.ch 6, 19 -- Organization: EPFL 6, 19 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 6, 19 -- MIME-Version: 1.0 6, 19 -- To: Microscopy-at-microscopy.com 6, 19 -- Subject: Re: annealing nanoparticles on c-film grids 6, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I never encountered any issues when performing annealing experiments using a conventional heating holder and a variety of support films. Silicon nitride films seem robust regardless of ultimate temperature and ramp rate. Carbon films, however, will start to show evidence of partial graphitization at just a few hundred C, and your NPs can become enveloped in sheets of graphitic-like carbon. This can be a big problem if you're studying a process which requires matter transport to or from the particle, as presumably the graphitic sheets will impede this transport.
You also need to keep in mind that your metal grid can generate NPs. See: Z. Zhang and D. Su, Behaviour of TEM metal-grids during in-situ heating experiments. Ultramicroscopy 109, 766 (2009).
Good luck, Chris Virginia Tech
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From reasonable-meds5-at-cablebg.net Fri Jun 6 10:34:14 2014 Return-Path: {reasonable-meds5-at-cablebg.net} Received: from ico-PC.ddns.cablebg.net (130-204-91-165.2074901013.ddns.cablebg.net [130.204.91.165]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s56FYCSc020297 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 6 Jun 2014 10:34:13 -0500 Message-Id: {201406061534.s56FYCSc020297-at-ns.microscopy.com}
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Email: zhangyc-at-gmail.com Name: Yucheng Zhang
Organization: EMPA, Switzerland
Title-Subject: [Filtered] JOEL TEM heating holder
Message: Dear EM community, I am trying to using a JEOL TEM heating holder ((Model 652) on a JEOL 2200FS TEM microsope. The holder was originally used on a JEOL 2010 microscope but I suppose it is also compatible with the 2200FS microscope. However, when loading the holder (without heating it up), there always seems to be a vaccum leakage in the specimen chamber(PiG4), although the vacuum of the microscope column (PiG1) remains good. The two O-rings on the holder are checked properly and should not be the problem. I am wondering if someone can share some experience with the holder. Is the holder really compatible with the 2200FS microscope? If so, what could cause the vacuum crash in the specimen chamber? Any advice would be greatly appreciated. Thanks in advance.
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Email: sunxh163-at-gmail.com Name: Xuanhao Sun
Organization: University of Connecticut
Title-Subject: [Filtered] Manual for LKB 2088 Ultrotome V
Message: Hi,
Does anyone have a user/service manual for LKB 2088 Ultrotome V? We recently started to see chatter in our ultrathin sections. This seems to be caused by the instrument itself. Any advice on this matter will be appreciated!
Xuanhao Sun, Ph.D.
Bioscience Electron Microscopy Laboratory University of Connecticut BPB G06, Unit 3242 Storrs, CT 06269-3242
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Email: rich-at-tescan-usa.com Name: Richard Fiore
Organization: Tescan USA
Title-Subject: [Filtered] NanoDay and Electron Microscopy (EM) Workshop at the University of Maryland
Message: Dear EM and FIB Friends:
I would like to take this opportunity to invite you to the NanoDay and Electron Microscopy (EM) Workshop that will be held at the University of Maryland, College Park, Maryland on June 11, 12 and 13, 2014.
Below are some of the highlights for this meeting:
* Plenary talks on Nanoscience and nanotechnology
* EM Workshop covers a broad spectrum of newest electron microscopy and focus ion beam technology
* Platform presentations and poster session
* Live demos (both with lecture and also in the laboratory)
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Additional information can be found at http://www.nanocenter.umd.edu/nanoday/
Hope to see you next week.
Best regards,
The NISP Lab NanoCenter, UMD
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From drugs-cheapest14-at-kronos.com Sat Jun 7 08:28:38 2014 Return-Path: {drugs-cheapest14-at-kronos.com} Received: from smtp4.kronos.com ([193.201.90.28]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s57DSY6q010415 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 7 Jun 2014 08:28:35 -0500 Message-Id: {201406071328.s57DSY6q010415-at-ns.microscopy.com}
Hi Need some advice about the effect of sputtering twice the same sample for SEM. I had a delicate preparation (pre-embryo hatching from the zona pellucida) showing fine details in SEM. Then I removed some rubbish from the surface of the sample and gave it a second gold sputtering with exactly same settings but half the time of the first one. In the second observation the surface was like "fried" and fine details were lost. Please click here: http://www.eikonika.net/v2/download4.php (upper foto is the first sputtering, lower is the second) Looks like the surface was either blebbing or it got a heavy and uneven disposition of gold particles. I was surprised by such a strong detrimental effect from re-sputtering and wonder if anybody knows more about this phenomenon and how can be avoided or minimized. Thanks a lot yorgos
==============================Original Headers============================== 4, 22 -- From eikonika-at-otenet.gr Sun Jun 8 04:28:23 2014 4, 22 -- Received: from medusa.otenet.gr (smtp-out31.otenet.gr [83.235.69.31]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s589SMVo025461 4, 22 -- for {microscopy-at-microscopy.com} ; Sun, 8 Jun 2014 04:28:22 -0500 4, 22 -- Received: from pegasus (ppp-2-84-160-24.home.otenet.gr [2.84.160.24]) 4, 22 -- by medusa.otenet.gr (ESMTP) with SMTP 4, 22 -- for {microscopy-at-microscopy.com} ; Sun, 8 Jun 2014 12:28:21 +0300 (EEST) 4, 22 -- Message-ID: {99139A4348F4439C982FA2B69770C6E0-at-pegasus} 4, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr} 4, 22 -- To: {microscopy-at-microscopy.com} 4, 22 -- Subject: re-sputtering matters in SEM 4, 22 -- Date: Sun, 8 Jun 2014 12:28:18 +0300 4, 22 -- MIME-Version: 1.0 4, 22 -- Content-Type: text/plain; 4, 22 -- format=flowed; 4, 22 -- charset="iso-8859-1"; 4, 22 -- reply-type=original 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- X-Priority: 3 4, 22 -- X-MSMail-Priority: Normal 4, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 4, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 ==============================End of - Headers==============================
From canadian-meds2-at-bull.uk.com Sun Jun 8 06:43:24 2014 Return-Path: {canadian-meds2-at-bull.uk.com} Received: from smtp2.bull.uk.com ([46.227.184.110]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s58Bgx80013670 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 8 Jun 2014 06:43:20 -0500 Message-Id: {201406081143.s58Bgx80013670-at-ns.microscopy.com}
Hi Yorgos
I am not a biologist, but I have worked with all sorts of sputtering systems, and what you see in your second picture has nothing to do with sputter coating.
Your second picture would suggest to me that you have removed a surface layer and you are seeing underlying structures; which is quite fascinating but quite typical with SEM studies.
Enjoy
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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-----Original Message----- X-from: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr] Sent: 08 June 2014 10:34 To: protrain-at-emcourses.com
X-from: marshall-at-sfu.ca ()
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Email: marshall-at-sfu.ca Name: Dan
Organization: Simon Fraser University
Title-Subject: [Filtered] Looking for pinouts for Oxford INCA PentaFET x3 Model 7060
Message: Hello Listers: One of our local users has donated a Penta FET x3 to our lab. The corresponding PHA and other collection electronics were unfortunately junked a while ago. I would like to try and marry this PentaFET to an existing APTEC FlatPack power supply and PHA. I got very little help from Oxford and was informed "This is all I'm allowed to give out", after some scant information on 4 of the pins. At the moment this is what we have or have been able to figure out. If anyone can supply addditional information on signals/volages that should be coming out or going into the Oxford EDS DB15 pin connector it would be much appreciated. In fact information on a similar detector would probably be a big helps as well. Pin 1: Conditioner (15VDC used when conditioning detector?) Pin 2: Signal Out Pin 3: Not used Pin 4: Not used Pin 5: Substrate (substrate to the MOSFET, grounded?) Pin 6: Ground? Pin 7: Heater Pin 8: Restore (very important pin, no info) Pin 9: Signal return (signal ground?) Pin 10: Not used Pin 11: High Voltage - 500 VDC Pin 12: Not used Pin 13: Test Pulse (nothing known about this) Pin 14: +24 VDC Pin 15: -24 VDC
Any information would be much appreciated. Thanks, Dan
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Sun Jun 8 17:26:18 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s58MQIXa016753 16, 28 -- for {microscopy-at-microscopy.com} ; Sun, 8 Jun 2014 17:26:18 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 44B1C713C8E 16, 28 -- for {microscopy-at-microscopy.com} ; Sun, 8 Jun 2014 17:26:18 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id 9O2w8Rs9Oa6N for {microscopy-at-microscopy.com} ; 16, 28 -- Sun, 8 Jun 2014 17:26:06 -0500 (CDT) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 23369713C7C 16, 28 -- for {microscopy-at-microscopy.com} ; Sun, 8 Jun 2014 17:26:06 -0500 (CDT) 16, 28 -- Message-ID: {5394E2FD.2050907-at-microscopy.com} 16, 28 -- Date: Sun, 08 Jun 2014 17:26:05 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:Looking for pinouts for Oxford INCA PentaFET x3 Model 7060 16, 28 -- References: {201406081627.s58GRMSB008226-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201406081627.s58GRMSB008226-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201406081627.s58GRMSB008226-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From medications-popular13-at-if.ua Sun Jun 8 22:21:58 2014 Return-Path: {medications-popular13-at-if.ua} Received: from svarog4.pu.if.ua ([194.44.152.136]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s593LuID005692 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 8 Jun 2014 22:21:57 -0500 Message-Id: {201406090321.s593LuID005692-at-ns.microscopy.com}
Hello Steve
Thanks for your input. The problem occurs only after such delicate biological specimens pass again from the sputter coater. Of course "after" does not mean "because", and I keep you comment that it is not looking like a metal disposition. Rather the specimen surface is affected and it needs a second passage from the sputter coater for this to happen. Maybe the first observation in SEM renders the surface susceptible to subsequent metallization?
I use 10KV and a current beam ca 20ua that is 2-3 times lower than the factory recommended value, because I found that these settings prolong 4-5 times the filament's life. Also my sputter coating is not optimal because I don't use argon. Could these aberrations be involved in the problem? I think this is possible, especially since I had no comments from people using more orthodox settings (somebody must have tried second sputtering).
Probably the best way to tackle the issue is to use very short times of re-sputtering and see..
Have a good day
yorgos
----- Original Message ----- X-from: "Steve Chapman" {protrain-at-emcourses.com} To: {eikonika-at-otenet.gr} Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} Sent: Sunday, June 08, 2014 6:51 PM
Hello Yorgos, It seems to me, that your samples were stored in the high humidity before second sputter-coater run. In such conditions the biological tissues might be rehydrated and then their surface might collapse in the sputter-coated vacuum. This might result in structures you have seen after second sputter-coating.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Sun, 8 Jun 2014 04:46:08 -0500, eikonika-at-otenet.gr wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } Need some advice about the effect of sputtering twice the same sample } for SEM. I had a delicate preparation (pre-embryo hatching from the } zona pellucida) showing fine details in SEM. Then I removed some } rubbish from the surface of the sample and gave it a second gold } sputtering with exactly same settings but half the time of the first } one. In the second observation the surface was like "fried" and fine } details were lost. Please click here: } http://www.eikonika.net/v2/download4.php (upper foto is the first } sputtering, lower is the second) Looks like the surface was either } blebbing or it got a heavy and uneven disposition of gold particles. } I was surprised by such a strong detrimental effect from } re-sputtering and wonder if anybody knows more about this phenomenon } and how can be avoided or minimized. Thanks a lot } yorgos } } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.net www.aim.cat } ************************************ } } } ==============================Original } Headers============================== 4, 22 -- From } eikonika-at-otenet.gr Sun Jun 8 04:28:23 2014 4, 22 -- Received: from } medusa.otenet.gr (smtp-out31.otenet.gr [83.235.69.31]) 4, 22 -- } by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s589SMVo025461 4, 22 -- for {microscopy-at-microscopy.com} ; Sun, } 8 Jun 2014 04:28:22 -0500 4, 22 -- Received: from pegasus } (ppp-2-84-160-24.home.otenet.gr [2.84.160.24]) 4, 22 -- by } medusa.otenet.gr (ESMTP) with SMTP 4, 22 -- for } {microscopy-at-microscopy.com} ; Sun, 8 Jun 2014 12:28:21 +0300 (EEST) } 4, 22 -- Message-ID: {99139A4348F4439C982FA2B69770C6E0-at-pegasus} 4, 22 } -- From: "yorgos nikas" {eikonika-at-otenet.gr} 4, 22 -- To: } {microscopy-at-microscopy.com} 4, 22 -- Subject: re-sputtering matters } in SEM 4, 22 -- Date: Sun, 8 Jun 2014 12:28:18 +0300 4, 22 -- } MIME-Version: 1.0 4, 22 -- Content-Type: text/plain; } 4, 22 -- format=flowed; } 4, 22 -- charset="iso-8859-1"; } 4, 22 -- reply-type=original } 4, 22 -- Content-Transfer-Encoding: 7bit } 4, 22 -- X-Priority: 3 } 4, 22 -- X-MSMail-Priority: Normal } 4, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 } 4, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 40 -- From benada-at-biomed.cas.cz Mon Jun 9 03:59:49 2014 5, 40 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 5, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s598xmPT030099 5, 40 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 03:59:48 -0500 5, 40 -- X-ASG-Debug-ID: 1402304386-05011e12a142edc0001-4CH8be 5, 40 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id dBUVfJf0COrtjlHM (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO); Mon, 09 Jun 2014 10:59:46 +0200 (CEST) 5, 40 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 5, 40 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 5, 40 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) 5, 40 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 5, 40 -- (No client certificate requested) 5, 40 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 377CA2C80E3; 5, 40 -- Mon, 9 Jun 2014 10:59:46 +0200 (CEST) 5, 40 -- Date: Mon, 9 Jun 2014 10:59:44 +0200 5, 40 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 5, 40 -- To: eikonika-at-otenet.gr, {microscopy-at-microscopy.com} 5, 40 -- Subject: Re: [Microscopy] re-sputtering matters in SEM 5, 40 -- Message-ID: {20140609105944.17b5ed00-at-u117fs} 5, 40 -- X-ASG-Orig-Subj: Re: [Microscopy] re-sputtering matters in SEM 5, 40 -- In-Reply-To: {201406080946.s589k8Xh028453-at-ns.microscopy.com} 5, 40 -- References: {201406080946.s589k8Xh028453-at-ns.microscopy.com} 5, 40 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 5, 40 -- =?UTF-8?B?xIxS?= 5, 40 -- X-Mailer: Claws Mail 3.9.3 (GTK+ 2.24.10; i486-pc-linux-gnu) 5, 40 -- MIME-Version: 1.0 5, 40 -- Content-Type: text/plain; charset=US-ASCII 5, 40 -- Content-Transfer-Encoding: 7bit 5, 40 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 5, 40 -- X-Barracuda-Start-Time: 1402304386 5, 40 -- X-Barracuda-Encrypted: AES256-SHA 5, 40 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 5, 40 -- Received-SPF: none (biomed.cas.cz: benada-at-biomed.cas.cz does not designate permitted sender hosts) 5, 40 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 5, 40 -- X-Barracuda-BRTS-Status: 1 5, 40 -- X-Barracuda-Spam-Score: 0.00 5, 40 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 5, 40 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.6498 5, 40 -- Rule breakdown below 5, 40 -- pts rule name description 5, 40 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
For some difficult samples I often have to use multiple coatings, with different tilt angles, but only for short periods, say 30 seconds per coat. Multiple coating may build structure on a specimen, but that is usually only visible above 15,000X if carried out with care. In some laboratories the operators forget that even when coated specimens may charge, and the best solution is to lower the accelerating voltage as well as to coat. Modern instruments work very well at 5kV or lower, and if you continue to use the low emission currents that you mention that too helps reduce charge. The third route to operation at a lower charge rate is to use a spot size/probe current that is far smaller/lower than normal. For example working at 5,000X use a probe current that would be more suitable for 30,000X; all part of the small steps that you need to make to operate without the complexities of too much coating. If you have a TTL instrument move away from the upper detector to introduce more of the converted backscatter (SE3) to subdue charge.
Using air for sputtering does not make that much difference at low magnifications, it just means you will have a different coating on a dry day compared with a wet day. Long coating periods, particularly using old style high voltage sputter coaters, may cook the specimen! However the modern low voltage coaters should never reach anything like the same heat generation.
After all that I have said there is one sure route to determining the true "surface" structure of your specimen, and that is to use all of the tricks I have mentioned above and very low accelerating voltage; i.e. 1 or 2kV. Even if you are only able to look at the specimen for just a short time, it will give you an idea of what the true structure is. But remember you are now penetrating far less into the material so the structure may be totally different from your 10kV!
I never look at a specimen with a sputter coating until I have tried to see what it looks like and how it behaves using all of the low dose methods mentioned above.
May I say I love the grey scale that you have produced with your SEM pictures, for me the levels are very good.
Keep trying and I am sure you will see that sputter coating may not be your problem?
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
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-----Original Message----- X-from: yorgos nikas [mailto:eikonika-at-otenet.gr] Sent: 09 June 2014 07:09 To: protrain-at-emcourses.com Cc: Microscopical Soc of America
Hi Dan,
Responding thru the listserv as your email address keeps bouncing.
I have a non-functioning Oxford Inca Pentafet detector that might be useful in solving your pinout problem as it could be "destructively" deconstructed to trace some of the connections. Happy to send it to you if you wanted to pay shipping. I could probably take off the dewar so the thing would ship in a smaller box.
Let me know,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
On 08/06/2014 7:27 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: marshall-at-sfu.ca () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both marshall-at-sfu.ca as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: marshall-at-sfu.ca } Name: Dan } } Organization: Simon Fraser University } } Title-Subject: [Filtered] Looking for pinouts for Oxford INCA PentaFET x3 Model 7060 } } Message: Hello Listers: } One of our local users has donated a Penta FET x3 to our lab. The corresponding PHA and other } collection electronics were unfortunately junked a while ago. I would like to try and marry this } PentaFET to an existing APTEC FlatPack power supply and PHA. I got very little help from Oxford and } was informed "This is all I'm allowed to give out", after some scant information on 4 of the pins. } At the moment this is what we have or have been able to figure out. If anyone can supply addditional } information on signals/volages that should be coming out or going into the Oxford EDS DB15 pin } connector it would be much appreciated. } In fact information on a similar detector would probably be a big helps as well. } Pin 1: Conditioner (15VDC used when conditioning detector?) } Pin 2: Signal Out } Pin 3: Not used } Pin 4: Not used } Pin 5: Substrate (substrate to the MOSFET, grounded?) } Pin 6: Ground? } Pin 7: Heater } Pin 8: Restore (very important pin, no info) } Pin 9: Signal return (signal ground?) } Pin 10: Not used } Pin 11: High Voltage - 500 VDC } Pin 12: Not used } Pin 13: Test Pulse (nothing known about this) } Pin 14: +24 VDC } Pin 15: -24 VDC } } Any information would be much appreciated. } Thanks, Dan } } } } Login Host: 174.7.122.17 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } }
==============================Original Headers============================== 16, 22 -- From jehrman-at-mta.ca Mon Jun 9 07:19:47 2014 16, 22 -- Received: from smtpy.mta.ca (smtpy.mta.ca [138.73.1.86]) 16, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s59CJkQw006149 16, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 07:19:46 -0500 16, 22 -- Received: from host-22-169.mta.ca ([138.73.22.169]:50469) 16, 22 -- by smtpy.mta.ca with esmtpsa (TLSv1:DHE-RSA-AES128-SHA:128) 16, 22 -- (Exim 4.82) 16, 22 -- (envelope-from {jehrman-at-mta.ca} ) 16, 22 -- id 1WtyWk-0003hk-D0 16, 22 -- for Microscopy-at-microscopy.com; Mon, 09 Jun 2014 09:18:14 -0300 16, 22 -- Message-ID: {5395A661.8030201-at-mta.ca} 16, 22 -- Date: Mon, 09 Jun 2014 09:19:45 -0300 16, 22 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 16, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 16, 22 -- MIME-Version: 1.0 16, 22 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 16, 22 -- Subject: Re: [Microscopy] viaWWW:Looking for pinouts for Oxford INCA PentaFET 16, 22 -- x3 Model 7060 16, 22 -- References: {201406082227.s58MRM8X017280-at-ns.microscopy.com} 16, 22 -- In-Reply-To: {201406082227.s58MRM8X017280-at-ns.microscopy.com} 16, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Has anybody tried Digital Micrograph in a Windows 8.1 environment? I have heard mixed stories, and the agent in the Microsoft store insisted that anything that runs in Windows 7 will run in 8.1.
John Mardinly, ASU
==============================Original Headers============================== 4, 35 -- From John.Mardinly-at-asu.edu Mon Jun 9 14:41:38 2014 4, 35 -- Received: from bcnetw2.asu.edu (bcnetw2.asu.edu [149.169.2.72]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s59JfbvY005146 4, 35 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Jun 2014 14:41:38 -0500 4, 35 -- X-ASG-Debug-ID: 1402342896-064f8d243352f90001-FOsErg 4, 35 -- Received: from exhubw02.asurite.ad.asu.edu (exhubw02.asurite.ad.asu.edu [129.219.4.200]) by bcnetw2.asu.edu with ESMTP id 3KjQsfvslNTPVmtk (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Mon, 09 Jun 2014 12:41:36 -0700 (MST) 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.200 4, 35 -- X-ASG-Whitelist: Client 4, 35 -- Received: from EXMBW02.asurite.ad.asu.edu ([169.254.9.206]) by 4, 35 -- exhubw02.asurite.ad.asu.edu ([129.219.4.200]) with mapi id 14.03.0174.001; 4, 35 -- Mon, 9 Jun 2014 12:41:36 -0700 4, 35 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 35 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 4, 35 -- Subject: Digital Micrograph and Windows 8.1 4, 35 -- Thread-Topic: Digital Micrograph and Windows 8.1 4, 35 -- X-ASG-Orig-Subj: Digital Micrograph and Windows 8.1 4, 35 -- Thread-Index: Ac+EGtKULl0ZS+ACRtqyCx6SSbjFBA== 4, 35 -- Date: Mon, 9 Jun 2014 19:41:35 +0000 4, 35 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE94015F025D3-at-exmbw02.asurite.ad.asu.edu} 4, 35 -- Accept-Language: en-US 4, 35 -- Content-Language: en-US 4, 35 -- X-MS-Has-Attach: 4, 35 -- X-MS-TNEF-Correlator: 4, 35 -- x-originating-ip: [129.219.4.240] 4, 35 -- Content-Type: text/plain; charset="us-ascii" 4, 35 -- MIME-Version: 1.0 4, 35 -- X-Barracuda-Connect: exhubw02.asurite.ad.asu.edu[129.219.4.200] 4, 35 -- X-Barracuda-Start-Time: 1402342896 4, 35 -- X-Barracuda-Encrypted: AES128-SHA 4, 35 -- X-Barracuda-URL: http://149.169.2.72:8000/cgi-mod/mark.cgi 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 35 -- X-Barracuda-BRTS-Status: 1 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s59JfbvY005146 ==============================End of - Headers==============================
I'm running DM 2.11 (actually Gatan Microscopy Suite) under Win8.1. It runs fine. I will, however, dispute the agent's claim about ALL Win7 apps running OK under Win8.1
Cheers, Henk
On 6/9/2014 3:45 PM, John.Mardinly-at-asu.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Has anybody tried Digital Micrograph in a Windows 8.1 environment? I have heard mixed stories, and the agent in the Microsoft store insisted that anything that runs in Windows 7 will run in 8.1. } } John Mardinly, ASU } } } } ==============================Original Headers============================== } 4, 35 -- From John.Mardinly-at-asu.edu Mon Jun 9 14:41:38 2014 } 4, 35 -- Received: from bcnetw2.asu.edu (bcnetw2.asu.edu [149.169.2.72]) } 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s59JfbvY005146 } 4, 35 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Jun 2014 14:41:38 -0500 } 4, 35 -- X-ASG-Debug-ID: 1402342896-064f8d243352f90001-FOsErg } 4, 35 -- Received: from exhubw02.asurite.ad.asu.edu (exhubw02.asurite.ad.asu.edu [129.219.4.200]) by bcnetw2.asu.edu with ESMTP id 3KjQsfvslNTPVmtk (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Mon, 09 Jun 2014 12:41:36 -0700 (MST) } 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.200 } 4, 35 -- X-ASG-Whitelist: Client } 4, 35 -- Received: from EXMBW02.asurite.ad.asu.edu ([169.254.9.206]) by } 4, 35 -- exhubw02.asurite.ad.asu.edu ([129.219.4.200]) with mapi id 14.03.0174.001; } 4, 35 -- Mon, 9 Jun 2014 12:41:36 -0700 } 4, 35 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 4, 35 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} } 4, 35 -- Subject: Digital Micrograph and Windows 8.1 } 4, 35 -- Thread-Topic: Digital Micrograph and Windows 8.1 } 4, 35 -- X-ASG-Orig-Subj: Digital Micrograph and Windows 8.1 } 4, 35 -- Thread-Index: Ac+EGtKULl0ZS+ACRtqyCx6SSbjFBA== } 4, 35 -- Date: Mon, 9 Jun 2014 19:41:35 +0000 } 4, 35 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE94015F025D3-at-exmbw02.asurite.ad.asu.edu} } 4, 35 -- Accept-Language: en-US } 4, 35 -- Content-Language: en-US } 4, 35 -- X-MS-Has-Attach: } 4, 35 -- X-MS-TNEF-Correlator: } 4, 35 -- x-originating-ip: [129.219.4.240] } 4, 35 -- Content-Type: text/plain; charset="us-ascii" } 4, 35 -- MIME-Version: 1.0 } 4, 35 -- X-Barracuda-Connect: exhubw02.asurite.ad.asu.edu[129.219.4.200] } 4, 35 -- X-Barracuda-Start-Time: 1402342896 } 4, 35 -- X-Barracuda-Encrypted: AES128-SHA } 4, 35 -- X-Barracuda-URL: http://149.169.2.72:8000/cgi-mod/mark.cgi } 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu } 4, 35 -- X-Barracuda-BRTS-Status: 1 } 4, 35 -- Content-Transfer-Encoding: 8bit } 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s59JfbvY005146 } ==============================End of - Headers============================== }
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Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
I was a bit hasty in my response to John. I should have indicated that I'm running the OFF-LINE version of DM. Note that the device drivers that talk to the hardware may or may not work under Win8.
Cheers, Henk
On 6/9/2014 3:52 PM, colijn.1-at-osu.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi John, } } I'm running DM 2.11 (actually Gatan Microscopy Suite) under Win8.1. It } runs fine. I will, however, dispute the agent's claim about ALL Win7 } apps running OK under Win8.1 } } Cheers, } Henk } } } On 6/9/2014 3:45 PM, John.Mardinly-at-asu.edu wrote: } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Has anybody tried Digital Micrograph in a Windows 8.1 environment? I have heard mixed stories, and the agent in the Microsoft store insisted that anything that runs in Windows 7 will run in 8.1. } } } } John Mardinly, ASU } } } } } } } } ==============================Original Headers============================== } } 4, 35 -- From John.Mardinly-at-asu.edu Mon Jun 9 14:41:38 2014 } } 4, 35 -- Received: from bcnetw2.asu.edu (bcnetw2.asu.edu [149.169.2.72]) } } 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s59JfbvY005146 } } 4, 35 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Jun 2014 14:41:38 -0500 } } 4, 35 -- X-ASG-Debug-ID: 1402342896-064f8d243352f90001-FOsErg } } 4, 35 -- Received: from exhubw02.asurite.ad.asu.edu (exhubw02.asurite.ad.asu.edu [129.219.4.200]) by bcnetw2.asu.edu with ESMTP id 3KjQsfvslNTPVmtk (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Mon, 09 Jun 2014 12:41:36 -0700 (MST) } } 4, 35 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } } 4, 35 -- X-Barracuda-Apparent-Source-IP: 129.219.4.200 } } 4, 35 -- X-ASG-Whitelist: Client } } 4, 35 -- Received: from EXMBW02.asurite.ad.asu.edu ([169.254.9.206]) by } } 4, 35 -- exhubw02.asurite.ad.asu.edu ([129.219.4.200]) with mapi id 14.03.0174.001; } } 4, 35 -- Mon, 9 Jun 2014 12:41:36 -0700 } } 4, 35 -- From: John Mardinly {John.Mardinly-at-asu.edu} } } 4, 35 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} } } 4, 35 -- Subject: Digital Micrograph and Windows 8.1 } } 4, 35 -- Thread-Topic: Digital Micrograph and Windows 8.1 } } 4, 35 -- X-ASG-Orig-Subj: Digital Micrograph and Windows 8.1 } } 4, 35 -- Thread-Index: Ac+EGtKULl0ZS+ACRtqyCx6SSbjFBA== } } 4, 35 -- Date: Mon, 9 Jun 2014 19:41:35 +0000 } } 4, 35 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE94015F025D3-at-exmbw02.asurite.ad.asu.edu} } } 4, 35 -- Accept-Language: en-US } } 4, 35 -- Content-Language: en-US } } 4, 35 -- X-MS-Has-Attach: } } 4, 35 -- X-MS-TNEF-Correlator: } } 4, 35 -- x-originating-ip: [129.219.4.240] } } 4, 35 -- Content-Type: text/plain; charset="us-ascii" } } 4, 35 -- MIME-Version: 1.0 } } 4, 35 -- X-Barracuda-Connect: exhubw02.asurite.ad.asu.edu[129.219.4.200] } } 4, 35 -- X-Barracuda-Start-Time: 1402342896 } } 4, 35 -- X-Barracuda-Encrypted: AES128-SHA } } 4, 35 -- X-Barracuda-URL: http://149.169.2.72:8000/cgi-mod/mark.cgi } } 4, 35 -- X-Virus-Scanned: by bsmtpd at asu.edu } } 4, 35 -- X-Barracuda-BRTS-Status: 1 } } 4, 35 -- Content-Transfer-Encoding: 8bit } } 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s59JfbvY005146 } } ==============================End of - Headers============================== } }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
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Email: bret.judson-at-bc.edu Name: Bret Judson
Organization: Boston College
Title-Subject: [Filtered] Philips CM10
Message: To all,
We recently donated our Philips CM10 and there were some items left over that I would like to see sent to a new home.
3 manuals: Electronics CM10/CM12 Parts 1,2, and 3 (2 manuals) and Mechanical CM10/CM12 (1 manual)
2 exchange units and accessories.
These items are free.
Best regards,
Bret Erase this text and type your question here
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: University of Idaho
Title-Subject: [Filtered] Asphalt samples in SEM
Message: Greetings,
Have a request to run natural asphalt samples on the SEM for imaging and EDS microanalysis. Customer [astrobiologist] is interested framboidal pyrite as a biomarker in samples from an asphalt lake. He describes samples as "gooey" which gives me pause.
I am in need of guidance from anyone who has experience with asphalt or tar samples on the SEM? Any precautions, caveats, or sample prep tips.
Any assistance or recommended references would be greatly appreciated!
Tom Williams Electron Microscopy Center University of Idaho
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jun 9 18:14:59 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s59NExd8000718 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:59 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id ABE03714944 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:59 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id Cg7dOH75DuwB for {microscopy-at-microscopy.com} ; 17, 28 -- Mon, 9 Jun 2014 18:14:55 -0500 (CDT) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 9AB52714937 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:55 -0500 (CDT) 17, 28 -- Message-ID: {53963FEF.7050901-at-microscopy.com} 17, 28 -- Date: Mon, 09 Jun 2014 18:14:55 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Asphalt samples in SEM 17, 28 -- References: {201406091855.s59It3Ya003087-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201406091855.s59It3Ya003087-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201406091855.s59It3Ya003087-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
If it is gooey, expect a lot of outgassing and possible -- even probable -- contamination. Can you get him to run a specimen through a GCMS and determine what volatiles are present in what concentrations?
Mike Andrews, W5EGO WWME Oklahoma area executive team
} On Jun 9, 2014, at 6:43 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: tomw-at-uidaho.edu () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both tomw-at-uidaho.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: tomw-at-uidaho.edu } Name: Tom Williams } } Organization: University of Idaho } } Title-Subject: [Filtered] Asphalt samples in SEM } } Message: Greetings, } } Have a request to run natural asphalt samples on the SEM for imaging and EDS microanalysis. } Customer [astrobiologist] is interested framboidal pyrite as a biomarker in samples from an asphalt } lake. He describes samples as "gooey" which gives me pause. } } I am in need of guidance from anyone who has experience with asphalt or tar samples on the SEM? Any } precautions, caveats, or sample prep tips. } } Any assistance or recommended references would be greatly appreciated! } } Tom Williams } Electron Microscopy Center } University of Idaho } } } } Login Host: 129.101.62.35 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original Headers============================== } 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jun 9 18:14:59 2014 } 17, 28 -- Received: from znl.com ([206.69.208.20]) } 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s59NExd8000718 } 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:59 -0500 } 17, 28 -- Received: from localhost (localhost [127.0.0.1]) } 17, 28 -- by znl.com (Postfix) with ESMTP id ABE03714944 } 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:59 -0500 (CDT) } 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 17, 28 -- Received: from znl.com ([127.0.0.1]) } 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 17, 28 -- with ESMTP id Cg7dOH75DuwB for {microscopy-at-microscopy.com} ; } 17, 28 -- Mon, 9 Jun 2014 18:14:55 -0500 (CDT) } 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) } 17, 28 -- by znl.com (Postfix) with ESMTPA id 9AB52714937 } 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:55 -0500 (CDT) } 17, 28 -- Message-ID: {53963FEF.7050901-at-microscopy.com} } 17, 28 -- Date: Mon, 09 Jun 2014 18:14:55 -0500 } 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 } 17, 28 -- MIME-Version: 1.0 } 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 17, 28 -- Subject: viaWWW:Asphalt samples in SEM } 17, 28 -- References: {201406091855.s59It3Ya003087-at-ns.microscopy.com} } 17, 28 -- In-Reply-To: {201406091855.s59It3Ya003087-at-ns.microscopy.com} } 17, 28 -- X-Forwarded-Message-Id: {201406091855.s59It3Ya003087-at-ns.microscopy.com} } 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 17, 28 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
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Dissolve a small amount of the asphalt material in xylene (this may take an hour or two; mix every once in awhile).
Filter the dissolved asphalt material through a steel (larger particles 50-500 um) or aluminum (smaller 0.5-20 um pore) filter. [you'll clog the small ones quickly; but that's ok since you're looking for semi-quant data].
[Alternatively use a soxhlet extraction system]
Wash it again in xylene.
If there is any pyrite with the carbonates, you should be able to see it in either a Reflected Polarized Light Microscopy or in the SEM.
2. Ash & Mount
Ash in muffle furnace at 350-500C for 0.5-3 hrs.
Mount the residue on a stub.
Coat for HV SEM or not for LV SEM.
3. Also, you may want to get a copy of:
Greer: Coal Microstructure & the Significance of Pyrite Inclusions, SEM, Vol I, 79-93, 1977.
Tony
Ref: Havics, A: Microscopy of Some Bitumen-, Asphalt-, and Mastic-Based Building Materials. Inter/Micro-2013.
......................................... Andrew Anthony "Tony" Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: University of Idaho
Title-Subject: [Filtered] Asphalt samples in SEM
Message: Greetings,
Have a request to run natural asphalt samples on the SEM for imaging and EDS microanalysis. Customer [astrobiologist] is interested framboidal pyrite as a biomarker in samples from an asphalt lake. He describes samples as "gooey" which gives me pause.
I am in need of guidance from anyone who has experience with asphalt or tar samples on the SEM? Any precautions, caveats, or sample prep tips.
Any assistance or recommended references would be greatly appreciated!
Tom Williams Electron Microscopy Center University of Idaho
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jun 9 18:14:59 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s59NExd8000718 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:59 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id ABE03714944 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:59 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id Cg7dOH75DuwB for {microscopy-at-microscopy.com} ; 17, 28 -- Mon, 9 Jun 2014 18:14:55 -0500 (CDT) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 9AB52714937 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:55 -0500 (CDT) 17, 28 -- Message-ID: {53963FEF.7050901-at-microscopy.com} 17, 28 -- Date: Mon, 09 Jun 2014 18:14:55 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Asphalt samples in SEM 17, 28 -- References: {201406091855.s59It3Ya003087-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201406091855.s59It3Ya003087-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201406091855.s59It3Ya003087-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From discounted_drugs13-at-webteks.com Tue Jun 10 00:17:54 2014 Return-Path: {discounted_drugs13-at-webteks.com} Received: from mail4.webteks.com ([222.234.72.217]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5A5Hp2M022016 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 10 Jun 2014 00:17:52 -0500 Message-Id: {201406100517.s5A5Hp2M022016-at-ns.microscopy.com}
Can't claim having actually done imaging of tar in SEM, but I wouldn't dare to put asphalt into otherwise clean instrument unless there was a way to cool it prior to pumping and keep cooled during imaging (i.e. cryo-stage) to prevent evaporation of (and coating insides of chamber by) heavy volatiles...
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 6/9/2014 7:54 PM, udsd007-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } If it is gooey, expect a lot of outgassing and possible -- even probable -- contamination. Can you get him to run a specimen through a GCMS and determine what volatiles are present in what concentrations? } } Mike Andrews, W5EGO } WWME Oklahoma area executive team } } } On Jun 9, 2014, at 6:43 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } X-from: tomw-at-uidaho.edu () } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both tomw-at-uidaho.edu as well as the Microscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: tomw-at-uidaho.edu } } Name: Tom Williams } } } } Organization: University of Idaho } } } } Title-Subject: [Filtered] Asphalt samples in SEM } } } } Message: Greetings, } } } } Have a request to run natural asphalt samples on the SEM for imaging and EDS microanalysis. } } Customer [astrobiologist] is interested framboidal pyrite as a biomarker in samples from an asphalt } } lake. He describes samples as "gooey" which gives me pause. } } } } I am in need of guidance from anyone who has experience with asphalt or tar samples on the SEM? Any } } precautions, caveats, or sample prep tips. } } } } Any assistance or recommended references would be greatly appreciated! } } } } Tom Williams } } Electron Microscopy Center } } University of Idaho } } } } } } } } Login Host: 129.101.62.35 } } Listserver Email Form V - 20120416 } } --------------------------------------------------------------------------- } } } } } } -- } } =========================================== } } Do not reply to this message it is from } } the Microscopy Listserver NO-REPLY forwarding } } system. You should send a new message to } } } } Microscopy-at-Microscopy.com } } } } ============================================ } } } } ==============================Original Headers============================== } } 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jun 9 18:14:59 2014 } } 17, 28 -- Received: from znl.com ([206.69.208.20]) } } 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s59NExd8000718 } } 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:59 -0500 } } 17, 28 -- Received: from localhost (localhost [127.0.0.1]) } } 17, 28 -- by znl.com (Postfix) with ESMTP id ABE03714944 } } 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:59 -0500 (CDT) } } 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } } 17, 28 -- Received: from znl.com ([127.0.0.1]) } } 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } } 17, 28 -- with ESMTP id Cg7dOH75DuwB for {microscopy-at-microscopy.com} ; } } 17, 28 -- Mon, 9 Jun 2014 18:14:55 -0500 (CDT) } } 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) } } 17, 28 -- by znl.com (Postfix) with ESMTPA id 9AB52714937 } } 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:14:55 -0500 (CDT) } } 17, 28 -- Message-ID: {53963FEF.7050901-at-microscopy.com} } } 17, 28 -- Date: Mon, 09 Jun 2014 18:14:55 -0500 } } 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } } 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } } 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 } } 17, 28 -- MIME-Version: 1.0 } } 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 17, 28 -- Subject: viaWWW:Asphalt samples in SEM } } 17, 28 -- References: {201406091855.s59It3Ya003087-at-ns.microscopy.com} } } 17, 28 -- In-Reply-To: {201406091855.s59It3Ya003087-at-ns.microscopy.com} } } 17, 28 -- X-Forwarded-Message-Id: {201406091855.s59It3Ya003087-at-ns.microscopy.com} } } 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 17, 28 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 4, 38 -- From udsd007-at-gmail.com Mon Jun 9 18:52:49 2014 } 4, 38 -- Received: from mail-ig0-f177.google.com (mail-ig0-f177.google.com [209.85.213.177]) } 4, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s59NqnJE006692 } 4, 38 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2014 18:52:49 -0500 } 4, 38 -- Received: by mail-ig0-f177.google.com with SMTP id l13so4495408iga.4 } 4, 38 -- for {microscopy-at-microscopy.com} ; Mon, 09 Jun 2014 16:52:48 -0700 (PDT) } 4, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 38 -- d=gmail.com; s=20120113; } 4, 38 -- h=subject:references:from:content-type:in-reply-to:message-id:date:to } 4, 38 -- :content-transfer-encoding:mime-version; } 4, 38 -- bh=6yRnj+BYjxiTX9+IDpoyAV3smrII6s3a6X89Kuvxu5k=; } 4, 38 -- b=TnTFCnLFzXDsmPYnZnWGu7fPlf4UVws3n4pOHs87BYm9wmV5Zd4E4gFCUol4+Yr0Y2 } 4, 38 -- 4yuCR2wMhw9CVP8DvMvLlwrOWZxvV21F6pLjlcONHoinrBq+MeC2Ae8QuhrKiYu8hZw1 } 4, 38 -- 81qiM+l1jFjeCdb+ONMtmnl3RZlBv/yJZjx7QVxK5wgrJcgo4IR4g3KFEDGBx2GU2LEh } 4, 38 -- I3a9qV04oba+zWl8CPJOQucWSEz2ll3tTlhc94x+0PvXjCsKBuMa9R3QyRQjZ7WeyliK } 4, 38 -- 2u3h51wa0yRHklRVTpOvIUeZlv+jaS1b6YmYvp8sD5VGBkSBDhfdPV2b1qqPoPMp5zXT } 4, 38 -- irOw== } 4, 38 -- X-Received: by 10.43.151.7 with SMTP id kq7mr15228926icc.78.1402357968594; } 4, 38 -- Mon, 09 Jun 2014 16:52:48 -0700 (PDT) } 4, 38 -- Received: from [10.251.79.48] (mobile-166-147-101-021.mycingular.net. 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Thank you for your comments. As Oldrich says, re-hydration may be an issue, however specimens more than one year old have no signs of deterioration and humidity is generally low in Athens. But maybe the combinatiomn of some humidity and repeat sputterring change things. I have an old style coater (Hummer V) using high voltage and the specimen looked rather cooked (to use Steve's word) than anything else. Also there were two off line comments, one from a plant morphologists saying that some changes after re-coating occur even in the relatively hard plant surfaces. And a material scientists sayed that using gold without argon may etch the membrane. Since no problem occurs in the first coating even if it is a very long one, I feel that the combination of second sputtering plus an unknown factor are holding the answer. If anybody of you has soft animal tissue specimens, a gold target and can take the trouble to resputter a spare specimen, this will be of great help to understand what is going on. Cheers yorgos
PS: Steve, thank you for praising my gray scales. It took me almost two decades to understand that a low contrast micrograph contains much more information than a high contrast one, and if you wish, this information is easy to enhance later with a computer program
Original Message ----- X-from: "Oldrich Benada" {benada-at-biomed.cas.cz} To: {eikonika-at-otenet.gr} ; {microscopy-at-microscopy.com} Sent: Monday, June 09, 2014 11:59 AM
As a side note, please understand that the agent is paid to sell, not to tell the truth. If he lies and sells more, he'll be rewarded. If he says the truth and his sells drop, he'll get problems.
-------------------------------------------- On Mon, 6/9/14, John.Mardinly-at-asu.edu {John.Mardinly-at-asu.edu} wrote:
Subject: [Microscopy] Digital Micrograph and Windows 8.1 To: nizets2-at-yahoo.com Date: Monday, June 9, 2014, 9:53 PM
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Has anybody tried Digital Micrograph in a Windows 8.1 environment? I have heard mixed stories, and the agent in the Microsoft store insisted that anything that runs in Windows 7 will run in 8.1.
John Mardinly, ASU
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Well that was a blast from the past. I started working on an SEM for Raymond T. Greer in 1977. He oversaw my masters and doctorate work. I've seen quite a few framboids in my day, but they don't often come up in discussion.
Tony's comments are good. You probably want to isolate the pyrite from the asphalt. The organics would not be all that good for your SEM. They might be difficult to image except in an VP-SEM or E-SEM. Plus, you would have a lot more material to scan through trying to find the pyrite. You might as well eliminate the organics if you can and examine what's left.
Warren
-----Original Message----- X-from: ph2-at-sprynet.com [mailto:ph2-at-sprynet.com] Sent: Monday, June 09, 2014 9:20 PM To: Straszheim, Warren E [BIOTC]
Tom:
1. Dissolve & Filter
Dissolve a small amount of the asphalt material in xylene (this may take an hour or two; mix every once in awhile).
Filter the dissolved asphalt material through a steel (larger particles 50-500 um) or aluminum (smaller 0.5-20 um pore) filter. [you'll clog the small ones quickly; but that's ok since you're looking for semi-quant data].
[Alternatively use a soxhlet extraction system]
Wash it again in xylene.
If there is any pyrite with the carbonates, you should be able to see it in either a Reflected Polarized Light Microscopy or in the SEM.
2. Ash & Mount
Ash in muffle furnace at 350-500C for 0.5-3 hrs.
Mount the residue on a stub.
Coat for HV SEM or not for LV SEM.
3. Also, you may want to get a copy of:
Greer: Coal Microstructure & the Significance of Pyrite Inclusions, SEM, Vol I, 79-93, 1977.
Tony
Ref: Havics, A: Microscopy of Some Bitumen-, Asphalt-, and Mastic-Based Building Materials. Inter/Micro-2013.
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: University of Idaho
Title-Subject: [Filtered] Asphalt samples in SEM
Message: Greetings,
Have a request to run natural asphalt samples on the SEM for imaging and EDS microanalysis. Customer [astrobiologist] is interested framboidal pyrite as a biomarker in samples from an asphalt lake. He describes samples as "gooey" which gives me pause.
I am in need of guidance from anyone who has experience with asphalt or tar samples on the SEM? Any precautions, caveats, or sample prep tips.
Any assistance or recommended references would be greatly appreciated!
Tom Williams Electron Microscopy Center University of Idaho
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Message: I wanted to find out if anyone knows a reliable and trustworthy moving company in NC that would move a JEOL 1011. The move would only be less than 6 miles, it takes less than 10 minutes by car in heavy traffic, only 2 traffic lights no matter which way you take! But it still needs a careful and SAFE ride. Thanks in advance for the information. Connie
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Jun 11 07:24:59 2014 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5BCOxFU005892 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 07:24:59 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 115D6715BFB 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 07:24:59 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id BKUD3BHYHVYK for {microscopy-at-microscopy.com} ; 11, 28 -- Wed, 11 Jun 2014 07:24:54 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 23BFF715BE9 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 07:24:54 -0500 (CDT) 11, 28 -- Message-ID: {53984A96.5090300-at-microscopy.com} 11, 28 -- Date: Wed, 11 Jun 2014 07:24:54 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:Moving a JEOL 1011 11, 28 -- References: {201406101331.s5ADV0cE006997-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201406101331.s5ADV0cE006997-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201406101331.s5ADV0cE006997-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] Scripting workshop at M&M 2014
Message: To All:
For those attending M&M 2014, you may be interested in attending a free scripting workshop using the Digital Micrograph software at 1-5 pm, Sunday, August 3, in Ballroom C, at the Marriott Hotel, which is attached to the Connecticut Convention Center.
The workshop is an introduction to Digital Micrograph scripting intended for new users, occasional users and those wanting to revisit the basics of Digital Micrograph Scripting.
You can register online at: http://www.gatan.com/company/news/news06091401.php
See you in Hartford! Cheers.
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Jun 11 07:25:57 2014 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5BCPvd0006434 18, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 07:25:57 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id 05D93715C0A 18, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 07:25:57 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id GpAgk7IRHCmY for {microscopy-at-microscopy.com} ; 18, 28 -- Wed, 11 Jun 2014 07:25:54 -0500 (CDT) 18, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id 5D12B715BFD 18, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 07:25:54 -0500 (CDT) 18, 28 -- Message-ID: {53984AD2.7070405-at-microscopy.com} 18, 28 -- Date: Wed, 11 Jun 2014 07:25:54 -0500 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: viaWWW:DM Scripting workshop at M&M 2014 18, 28 -- References: {201406102109.s5AL9NHb007485-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201406102109.s5AL9NHb007485-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201406102109.s5AL9NHb007485-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I was wondering if anyone is using the K2 IND confocal attachment for white light reflected light microscopy. We purchased a used unit and are unable to get it working. Any help would be greatly appreciated.
Specifically, this is where we are:
The unit has a transfer lens and a transfer lens adjustment knob. According to the manual, there need to be the following transfer lens adjustment steps:
---------------- 1. In the CF1 position, adjust the focus to maximize the image intensity from a reflected light sample in the eyepieces.
2. Use a screwdriver to adjust the transfer lens adjustment until the pinholes of the stationary disc are visible. ----------------
We have a custom setup with infinity corrected Nikon lenses and a Nikon Optiphot trinocular head. The problem we are facing is that we can find a setting where we can see the pinholes, but in that configuration we are unable to find any microscope focus setting where we can clearly see the sample.
Any hints ?
regards, Stefan
==============================Original Headers============================== 10, 33 -- From dev.c0debabe-at-gmail.com Wed Jun 11 08:18:15 2014 10, 33 -- Received: from mail-wi0-f171.google.com (mail-wi0-f171.google.com [209.85.212.171]) 10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5BDIERM012283 10, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 08:18:15 -0500 10, 33 -- Received: by mail-wi0-f171.google.com with SMTP id n15so5066738wiw.16 10, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 06:18:12 -0700 (PDT) 10, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 33 -- d=gmail.com; s=20120113; 10, 33 -- h=message-id:date:from:user-agent:mime-version:to:subject 10, 33 -- :content-type:content-transfer-encoding; 10, 33 -- bh=tXdJzNl02RfFrLDiBVV6BCwqDiJHvJbs9Fdu7tP4uUg=; 10, 33 -- b=ouMd50CZZzlW1JwISwl0tOW3IQuy7wT5wnXAel5HkDP9CHP8qwHmG2alCxtDl9ECO6 10, 33 -- 5p1y2aE0tG/iKckiihwVEykvtfsTJ3Qxs58/QUPK8iXp8Jxmlj106LrFl5aYuCvCVGJJ 10, 33 -- /x3msNGyc/RzuXo2Vg2FkEilo9yjpLABD/HmlWg/TmGEBxIaf8FV/tBFznIndYbRSvY0 10, 33 -- 7a2ZkANQ0bQZSLO2UuSJbDF2aV/kEgawhS2xjRgsu6BZpbEHFptX1bjjTm+75hX+RdIc 10, 33 -- OjzyRVzykzexb1M8zR4NPd/Ed8nf8JZ9fjh0uamL09kEgDXhKUiorcqug+rqgDoa4Bdv 10, 33 -- jqDw== 10, 33 -- X-Received: by 10.194.119.34 with SMTP id kr2mr52531421wjb.34.1402492692132; 10, 33 -- Wed, 11 Jun 2014 06:18:12 -0700 (PDT) 10, 33 -- Received: from [128.130.60.31] (amnesiac.seclab.tuwien.ac.at. [128.130.60.31]) 10, 33 -- by mx.google.com with ESMTPSA id fh5sm27169271wic.9.2014.06.11.06.18.10 10, 33 -- for {Microscopy-at-microscopy.com} 10, 33 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 10, 33 -- Wed, 11 Jun 2014 06:18:11 -0700 (PDT) 10, 33 -- Message-ID: {5398570E.8010109-at-gmail.com} 10, 33 -- Date: Wed, 11 Jun 2014 15:18:06 +0200 10, 33 -- From: =?ISO-8859-1?Q?Stefan_Sch=F6nleitner?= {dev.c0debabe-at-gmail.com} 10, 33 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:24.0) Gecko/20100101 Thunderbird/24.2.0 10, 33 -- MIME-Version: 1.0 10, 33 -- To: Microscopy-at-microscopy.com 10, 33 -- Subject: K2 IND Confocal Attachment 10, 33 -- Content-Type: text/plain; charset=ISO-8859-1 10, 33 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From drugs_canadian15-at-spdop.ru Wed Jun 11 11:53:54 2014 Return-Path: {drugs_canadian15-at-spdop.ru} Received: from ppp95-165-226-50.pppoe.spdop.ru (ppp95-165-226-50.pppoe.spdop.ru [95.165.226.50]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5BGrmAf006861 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 11 Jun 2014 11:53:49 -0500 Message-Id: {201406111653.s5BGrmAf006861-at-ns.microscopy.com}
X-from: mlj55-at-drexel.edu ()
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Email: mlj55-at-drexel.edu Name: Michael Jablonski
Organization: Drexel University
Title-Subject: [Filtered] Joint Drexel University-Sandia National Laboratory Post-Doc Position Available
Message: We are looking for a post-doctoral researcher for a position working in in situ TEM at both Drexel University and Sandia National Laboratory. Please see the following link for details.
http://dcg.materials.drexel.edu/?page_id=394
Thank you, Michael Jablonski (on behalf of Dr. Mitra Taheri)
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==============================Original Headers============================== 13, 29 -- From microscopylistserver-noreply-at-microscopy.com Wed Jun 11 19:31:24 2014 13, 29 -- Received: from znl.com ([206.69.208.20]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5C0VOBI012349 13, 29 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 19:31:24 -0500 13, 29 -- Received: from localhost (localhost [127.0.0.1]) 13, 29 -- by znl.com (Postfix) with ESMTP id 37853716278 13, 29 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 19:31:24 -0500 (CDT) 13, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 29 -- Received: from znl.com ([127.0.0.1]) 13, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 29 -- with ESMTP id NyeF2JVv5nsm for {microscopy-at-microscopy.com} ; 13, 29 -- Wed, 11 Jun 2014 19:31:21 -0500 (CDT) 13, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 29 -- by znl.com (Postfix) with ESMTPA id 181F671626B 13, 29 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2014 19:31:21 -0500 (CDT) 13, 29 -- Message-ID: {5398F4D8.8090703-at-microscopy.com} 13, 29 -- Date: Wed, 11 Jun 2014 19:31:20 -0500 13, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 13, 29 -- MIME-Version: 1.0 13, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 29 -- Subject: viaWWW:Joint Drexel University-Sandia National Laboratory Post-Doc 13, 29 -- Position Available 13, 29 -- References: {201406111954.s5BJsfJD009199-at-ns.microscopy.com} 13, 29 -- In-Reply-To: {201406111954.s5BJsfJD009199-at-ns.microscopy.com} 13, 29 -- X-Forwarded-Message-Id: {201406111954.s5BJsfJD009199-at-ns.microscopy.com} 13, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The K2 technology was developed by Technical Instruments of San Francisco. I'd recommend that you get in touch with them.
Good hunting!
Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through Fall 2014. Call us today for a free training evaluation.
At 08:47 AM 6/11/2014, you wrote:
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==============================Original Headers============================== 13, 28 -- From bfoster-at-the-mip.com Wed Jun 11 23:43:07 2014 13, 28 -- Received: from barcelona.directrouter.com (barcelona.directrouter.com [72.249.107.2]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5C4h6hQ001109 13, 28 -- for {Microscopy-at-Microscopy.com} ; Wed, 11 Jun 2014 23:43:07 -0500 13, 28 -- Message-Id: {201406120443.s5C4h6hQ001109-at-ns.microscopy.com} 13, 28 -- Received: from 99-168-105-118.lightspeed.rcsntx.sbcglobal.net ([99.168.105.118]:60754 helo=Bs-PC.the-mip.com) 13, 28 -- by barcelona.directrouter.com with esmtpsa (TLSv1:DHE-RSA-AES256-SHA:256) 13, 28 -- (Exim 4.82) 13, 28 -- (envelope-from {bfoster-at-the-mip.com} ) 13, 28 -- id 1Wuwqw-0006aY-Fg; Wed, 11 Jun 2014 23:43:06 -0500 13, 28 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 28 -- Date: Wed, 11 Jun 2014 23:43:02 -0500 13, 28 -- To: dev.c0debabe-at-gmail.com, Microscopy-at-Microscopy.com 13, 28 -- From: Barbara Foster {bfoster-at-the-mip.com} 13, 28 -- Subject: Re: [Microscopy] K2 IND Confocal Attachment 13, 28 -- In-Reply-To: {201406111332.s5BDWwUp024622-at-ns.microscopy.com} 13, 28 -- References: {201406111332.s5BDWwUp024622-at-ns.microscopy.com} 13, 28 -- Mime-Version: 1.0 13, 28 -- Content-Type: text/plain; charset="us-ascii" 13, 28 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 13, 28 -- X-AntiAbuse: Primary Hostname - barcelona.directrouter.com 13, 28 -- X-AntiAbuse: Original Domain - microscopy.com 13, 28 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 13, 28 -- X-AntiAbuse: Sender Address Domain - the-mip.com 13, 28 -- X-Get-Message-Sender-Via: barcelona.directrouter.com: authenticated_id: bfoster-at-the-mip.com 13, 28 -- X-Source: 13, 28 -- X-Source-Args: 13, 28 -- X-Source-Dir: ==============================End of - Headers==============================
As many of you know for the last 3-5 years there has been at first a discrete and now a campaign about the safety of uranyl acetate.
As the only worldwide manufacturer of uranyl acetate this has caused us great concern since now many EM brokers are making unreasonable claims that they have a replacement or alternative to uranyl acetate when no such product exists.
As you all know there is a risk in using any product and uranyl acetate is no exception.
We are preparing an educational handling and instructional manual for the safe use of uranyl acetate in conjunction with other users in Europe and the US.
Since the material is slightly radioactive the safety officers have gone out of their way to condemn this product and this manual is an educational manual to assist the worker as well as the safety officer in the proper use and handling of uranyl acetate.
Anyone wishing to give us insight as to the positive as well as the negative interaction with their safety officers would be welcomed. We will not publish any names.
There has been considerable hoopla regarding anti-terrorism in Europe and the US, let me assure everyone that you could not light a fire cracker with uranyl acetate.
Please send any comments to abesenyo-at-ibilabs.com
Thank you for your Time Alex Besenyo
==============================Original Headers============================== 14, 42 -- From bioanalytics-at-ibilabs.com Thu Jun 12 09:42:27 2014 14, 42 -- Received: from linux36.domainnameservers.net (linux36.domainnameservers.net [173.193.173.82]) 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5CEgQHl027631 14, 42 -- for {Microscopy-at-Microscopy.Com} ; Thu, 12 Jun 2014 09:42:27 -0500 14, 42 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; d=ibilabs.com; s=default; 14, 42 -- h=Content-Transfer-Encoding:Content-Type:MIME-Version:To:From:Subject:Date:Message-ID; bh=xsGf+v8lnrTU7k3Wqnc49mD6A/I8KhN36pAQvOuCv3g=; 14, 42 -- b=KGUBG/Jb7Hgqp0os1wDAV+VWyJufq4J4hcbsdUz+BwLN0IdfnxdC6qdiK5DLFCDVBTQaw/qUGrH3/uUk7cw8bL/vb12oM/J1rXaM2BEr9c+JKn8PjS0+9hNSoqOChpzrC5BFuQYCKMrKnYxYYBB51PJN+4yQ7DMpWs1NhM/pS50=; 14, 42 -- Received: from localhost.localdomain ([127.0.0.1]:57938 helo=linux36.domainnameservers.net) 14, 42 -- by linux36.domainnameservers.net with esmtpa (Exim 4.82) 14, 42 -- (envelope-from {bioanalytics-at-ibilabs.com} ) 14, 42 -- id 1Wv6Ch-00055O-PZ 14, 42 -- for Microscopy-at-Microscopy.Com; Thu, 12 Jun 2014 10:42:11 -0400 14, 42 -- Received: from 70.46.108.123 ([70.46.108.123]) 14, 42 -- (SquirrelMail authenticated user bioanalytics-at-ibilabs.com) 14, 42 -- by linux36.domainnameservers.net with HTTP; 14, 42 -- Thu, 12 Jun 2014 10:42:11 -0400 14, 42 -- Message-ID: {811344296683916a8ad36d7c5ea89eab.squirrel-at-linux36.domainnameservers.net} 14, 42 -- Date: Thu, 12 Jun 2014 10:42:11 -0400 14, 42 -- Subject: Is uranyl acetate at risk or mandatory to do the job properly? 14, 42 -- From: "IBILABS" {bioanalytics-at-ibilabs.com} 14, 42 -- To: Microscopy-at-Microscopy.Com 14, 42 -- User-Agent: SquirrelMail/1.5.2 [SVN] 14, 42 -- MIME-Version: 1.0 14, 42 -- Content-Type: text/plain;charset=iso-8859-1 14, 42 -- Content-Transfer-Encoding: 8bit 14, 42 -- X-Buzix-MailScanner-Information: Please contact the ISP for more information 14, 42 -- X-Buzix-MailScanner-ID: 1Wv6Ch-00055O-PZ 14, 42 -- X-Buzix-MailScanner: Found to be clean 14, 42 -- X-Buzix-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 14, 42 -- score=-2.9, required 5, autolearn=not spam, ALL_TRUSTED -1.00, 14, 42 -- BAYES_00 -1.90) 14, 42 -- X-Buzix-MailScanner-From: bioanalytics-at-ibilabs.com 14, 42 -- X-Spam-Status: No 14, 42 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 14, 42 -- X-AntiAbuse: Primary Hostname - linux36.domainnameservers.net 14, 42 -- X-AntiAbuse: Original Domain - microscopy.com 14, 42 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 14, 42 -- X-AntiAbuse: Sender Address Domain - ibilabs.com 14, 42 -- X-Get-Message-Sender-Via: linux36.domainnameservers.net: authenticated_id: bioanalytics-at-ibilabs.com 14, 42 -- X-Source: 14, 42 -- X-Source-Args: 14, 42 -- X-Source-Dir: ==============================End of - Headers==============================
From drugs-best3-at-mts.ru Thu Jun 12 10:14:59 2014 Return-Path: {drugs-best3-at-mts.ru} Received: from 162.gprs.mts.ru (162.gprs.mts.ru [213.87.138.162] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5CFEt3W014097 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 12 Jun 2014 10:14:56 -0500 Message-Id: {201406121514.s5CFEt3W014097-at-ns.microscopy.com}
Greetings,
The 2014 Microscopy and Microanalysis meetings in Hartford are less than 2 months away and there are still a few slots left for student bursaries* to help with things such as providing support in the different symposia, staffing the volunteer office, monitoring use of the Internet Café, and helping with vendor tutorial sign-up. MSA*s student bursary program is designed to encourage students to attend the meetings by helping to defray some of the costs and giving them an opportunity to meet and interact with the established microscopy community.
Bursaries will earn $10/hour for assisting with any of the tasks mentioned above (paid by check at the end of the meetings). There is an added bonus of a meeting shirt and $10 cash for each morning and/or afternoon session worked to help with meals.
For those *non-students* volunteers are also needed to help with the above mentioned meeting activities. Although not paid on an hourly basis as the student bursaries, volunteers do receive a meeting shirt and the same cash allotment to help with meals. They also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
We can*t do it without help from you, so please consider serving as a student bursary (or volunteer). If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact me.
Don*t forget early registration deadline is June 23!
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-3019 alawrence-at-i2at.msstate.edu
*Bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.
==============================Original Headers============================== 19, 23 -- From ALawrence-at-i2at.msstate.edu Thu Jun 12 13:01:40 2014 19, 23 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 19, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5CI1dvD016929 19, 23 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2014 13:01:39 -0500 19, 23 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197]) 19, 23 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id s5CI1cI6026756 19, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 19, 23 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2014 13:01:38 -0500 19, 23 -- X-Sender: {} 19, 23 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu 19, 23 -- with Novell_GroupWise; Thu, 12 Jun 2014 13:01:38 -0500 19, 23 -- Message-Id: {5399A4B0020000E6000E1981-at-mailhost.groupwise.msstate.edu} 19, 23 -- X-Mailer: Novell GroupWise Internet Agent 12.0.2 19, 23 -- Date: Thu, 12 Jun 2014 13:01:36 -0500 19, 23 -- From: "Amanda Lawrence" {ALawrence-at-i2at.msstate.edu} 19, 23 -- To: {microscopy-at-microscopy.com} 19, 23 -- Subject: Assisting at M&M 2014 19, 23 -- References: {5399A343020000E6000E197A-at-mailhost.groupwise.msstate.edu} 19, 23 -- {5399A4B0020000E6000E1981-at-mailhost.groupwise.msstate.edu} 19, 23 -- Mime-Version: 1.0 19, 23 -- Content-Type: text/plain; charset=ISO-8859-15 19, 23 -- Content-Transfer-Encoding: 8bit 19, 23 -- Content-Disposition: inline ==============================End of - Headers==============================
From drugstore_affordable15-at-wikitravel.org Thu Jun 12 16:02:50 2014 Return-Path: {drugstore_affordable15-at-wikitravel.org} Received: from mail2.wikitravel.org (localhost [117.2.173.53] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5CL2fBb021826 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 12 Jun 2014 16:02:45 -0500 Message-Id: {201406122102.s5CL2fBb021826-at-ns.microscopy.com}
Well, It's empty mail bag Friday, so let me ask a question. Wired has an article on macrophotography of inclusion in minerals. They very nice images.
I'm not a trained geologist, but is it possible to have carbonate inclusions in quartz?
Stay safe............ Frank
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==============================Original Headers============================== 9, 28 -- From frank_karl-at-ardl.com Fri Jun 13 07:14:30 2014 9, 28 -- Received: from cal1-mh745b.smtproutes.com (cal1-mh745b.smtproutes.com [208.70.89.155]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5DCESlu003478 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 07:14:28 -0500 9, 28 -- X-Katharion-ID: 1402661651.50280.cal1-mh745 9, 28 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by 9, 28 -- cal1-mh745.smtproutes.com [(192.69.16.67)] with ESMTP via TCP 9, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 13 Jun 2014 12:14:11 +0000 9, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 9, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Fri, 13 9, 28 -- Jun 2014 08:14:11 -0400 9, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 9, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 9, 28 -- {microscopy-at-microscopy.com} 9, 28 -- Date: Fri, 13 Jun 2014 08:14:11 -0400 9, 28 -- Subject: inclusions in minerals 9, 28 -- Thread-Topic: inclusions in minerals 9, 28 -- Thread-Index: Ac+HAPvNwJX+EP+DQmSWwZnNPSmCDA== 9, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C04B3576E-at-exchange2k7.ad.ardl.com} 9, 28 -- Accept-Language: en-US 9, 28 -- Content-Language: en-US 9, 28 -- X-MS-Has-Attach: 9, 28 -- X-MS-TNEF-Correlator: 9, 28 -- acceptlanguage: en-US 9, 28 -- Content-Type: text/plain; charset="us-ascii" 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5DCESlu003478 ==============================End of - Headers==============================
From discounted_medications14-at-kahoe.com Fri Jun 13 07:51:37 2014 Return-Path: {discounted_medications14-at-kahoe.com} Received: from mail2.kahoe.com (94.41.235.204.dynamic.neft.ufanet.ru [94.41.235.204]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5DCpXbg022881 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 13 Jun 2014 07:51:35 -0500 Message-Id: {201406131251.s5DCpXbg022881-at-ns.microscopy.com}
Dear listers,
We have film holders/cassettes from two TEMs, the JEOL JEM 1200EXII and the JEM 1400. We are doing all digital imaging and have no need to keep them. We will be surplussing them through the government as required. Government facilities have first dibs on these but if none reply other educational facilities can have them for the cost of shipping.
Please contact me off line if you can use them.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH.
Pat Patricia Stranen Connelly NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Jun 13 17:43:40 2014 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5DMhekD016050 15, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 17:43:40 -0500 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id B4F637178F7 15, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 17:43:40 -0500 (CDT) 15, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id QfPuuMK1Mo7H for {microscopy-at-microscopy.com} ; 15, 28 -- Fri, 13 Jun 2014 17:43:27 -0500 (CDT) 15, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 1C39B7178E4 15, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 17:43:27 -0500 (CDT) 15, 28 -- Message-ID: {539B7E8E.6040409-at-microscopy.com} 15, 28 -- Date: Fri, 13 Jun 2014 17:43:26 -0500 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:cryoEM Job position 15, 28 -- References: {201406132043.s5DKhZ3P030609-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201406132043.s5DKhZ3P030609-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201406132043.s5DKhZ3P030609-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 15, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I was asked if it would be possible to use TEM-EDS to map locations of Zinc ions incorporated into cellulose nano fibers (CNF). These fibers can be quite long but are often less than 20nm in width. At this point I have no idea as to the amount of zinc that could be incorporated into the fibers or the distribution. Does anyone have an idea as to the spacial resolution limits of mapping nano-scale ZnO on CNFs?
I am not sure that TEM-EDS is the way to go or if another technique, such as AFM, would work better to confirm the presence and distribution of the Zn on the CNFs.
Debby
Debra Sherman, Founder & CSO DS imaging LLC Purdue Technology Center 1281 Win Hentschel Blvd West Lafayette, IN 47906 E-mail: debby.sherman-at-dsimagingllc.com www.dsimagingllc.com Mobile: 765-418-8540
==============================Original Headers============================== 7, 31 -- From dsherman-at-purdue.edu Fri Jun 13 19:02:59 2014 7, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5E02wjQ003265 7, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 19:02:58 -0500 7, 31 -- Received: from WPVEXCHUB04.purdue.lcl (wpvexchub04.itap.purdue.edu [172.30.136.70]) 7, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s5E02wr0014408 7, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 20:02:58 -0400 7, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([169.254.2.206]) by 7, 31 -- WPVEXCHUB04.purdue.lcl ([172.30.136.70]) with mapi id 14.03.0174.001; Fri, 13 7, 31 -- Jun 2014 20:02:58 -0400 7, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 7, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 31 -- Subject: spacial resolution of ZN 7, 31 -- Thread-Topic: spacial resolution of ZN 7, 31 -- Thread-Index: AQHPh2P/nK/2QiJHi0qkNxxx21dc8g== 7, 31 -- Date: Sat, 14 Jun 2014 00:02:57 +0000 7, 31 -- Message-ID: {CFC1096F.1EEDE%dsherman-at-purdue.edu} 7, 31 -- Accept-Language: en-US 7, 31 -- Content-Language: en-US 7, 31 -- X-MS-Has-Attach: 7, 31 -- X-MS-TNEF-Correlator: 7, 31 -- user-agent: Microsoft-MacOutlook/14.4.2.140509 7, 31 -- x-originating-ip: [24.14.170.252] 7, 31 -- Content-Type: text/plain; charset="us-ascii" 7, 31 -- Content-ID: {200F6AED770E76459E61997C221F8E94-at-exchange.purdue.edu} 7, 31 -- MIME-Version: 1.0 7, 31 -- X-PMX-Version: 6.0.2.2308539 7, 31 -- X-PerlMx-URL-Scanned: Yes 7, 31 -- X-PerlMx-Virus-Scanned: Yes 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5E02wjQ003265 ==============================End of - Headers==============================
From canadian-drugs6-at-tpnet.pl Sat Jun 14 06:40:07 2014 Return-Path: {canadian-drugs6-at-tpnet.pl} Received: from vi146.internetdsl.tpnet.pl (vi146.internetdsl.tpnet.pl [80.55.164.146]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5EBdwLF027801 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 14 Jun 2014 06:39:59 -0500 Message-Id: {201406141139.s5EBdwLF027801-at-ns.microscopy.com}
My initial thoughts are that the first back-of-the-envelope calculation I would look at is minimum mass fraction and minimum detectable mass -- see Carter & Williams, 2nd ed., chapter 36.
If the Zn loading is low and they are not agglomerated into dense clusters, the whole thing might be undetectable before spatial resolution ever comes into it.
However, if you do get good ZnO clusters (~ nms), the carbonaceous nanofibers are probably small enough and poorly scattering enough that spatial resolution will be ~ probe size, I would guess. (But run the calculation -- also Chap. 36 in CW2)
A FEG with a big X-ray detector is indicated. HAADF might do the job, too, because Zn will scatter much better than carbon.
As an example, I've mapped ~hundreds of PPM aluminum in a steel, but only because it was agglomerated into aluminum oxide precipitates, and because I used a Titan w/ChemiSTEM instrument [Parish et al., J. Nucl. Mater., V445, P. 251, 2014].
Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Friday, June 13, 2014 8:22 PM To: Parish, Chad M.
Hi all,
I was asked if it would be possible to use TEM-EDS to map locations of Zinc ions incorporated into cellulose nano fibers (CNF). These fibers can be quite long but are often less than 20nm in width. At this point I have no idea as to the amount of zinc that could be incorporated into the fibers or the distribution. Does anyone have an idea as to the spacial resolution limits of mapping nano-scale ZnO on CNFs?
I am not sure that TEM-EDS is the way to go or if another technique, such as AFM, would work better to confirm the presence and distribution of the Zn on the CNFs.
Debby
Debra Sherman, Founder & CSO DS imaging LLC Purdue Technology Center 1281 Win Hentschel Blvd West Lafayette, IN 47906 E-mail: debby.sherman-at-dsimagingllc.com www.dsimagingllc.com Mobile: 765-418-8540
==============================Original Headers============================== 7, 31 -- From dsherman-at-purdue.edu Fri Jun 13 19:02:59 2014 7, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5E02wjQ003265 7, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 19:02:58 -0500 7, 31 -- Received: from WPVEXCHUB04.purdue.lcl (wpvexchub04.itap.purdue.edu [172.30.136.70]) 7, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s5E02wr0014408 7, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 20:02:58 -0400 7, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([169.254.2.206]) by 7, 31 -- WPVEXCHUB04.purdue.lcl ([172.30.136.70]) with mapi id 14.03.0174.001; Fri, 13 7, 31 -- Jun 2014 20:02:58 -0400 7, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 7, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 31 -- Subject: spacial resolution of ZN 7, 31 -- Thread-Topic: spacial resolution of ZN 7, 31 -- Thread-Index: AQHPh2P/nK/2QiJHi0qkNxxx21dc8g== 7, 31 -- Date: Sat, 14 Jun 2014 00:02:57 +0000 7, 31 -- Message-ID: {CFC1096F.1EEDE%dsherman-at-purdue.edu} 7, 31 -- Accept-Language: en-US 7, 31 -- Content-Language: en-US 7, 31 -- X-MS-Has-Attach: 7, 31 -- X-MS-TNEF-Correlator: 7, 31 -- user-agent: Microsoft-MacOutlook/14.4.2.140509 7, 31 -- x-originating-ip: [24.14.170.252] 7, 31 -- Content-Type: text/plain; charset="us-ascii" 7, 31 -- Content-ID: {200F6AED770E76459E61997C221F8E94-at-exchange.purdue.edu} 7, 31 -- MIME-Version: 1.0 7, 31 -- X-PMX-Version: 6.0.2.2308539 7, 31 -- X-PerlMx-URL-Scanned: Yes 7, 31 -- X-PerlMx-Virus-Scanned: Yes 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5E02wjQ003265 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 39 -- From parishcm-at-ornl.gov Mon Jun 16 06:59:29 2014 23, 39 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.14]) 23, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5GBxTsv006630 23, 39 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jun 2014 06:59:29 -0500 23, 39 -- X-SG: RELAYLIST 23, 39 -- X-IronPort-AV: E=Sophos;i="5.01,486,1400040000"; 23, 39 -- d="scan'208";a="81216574" 23, 39 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 23, 39 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 16 Jun 2014 07:59:28 -0400 23, 39 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151]) 23, 39 -- (using TLSv1 with cipher RC4-SHA (128/128 bits)) 23, 39 -- (No client certificate requested) 23, 39 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id A91C6100306; 23, 39 -- Mon, 16 Jun 2014 07:59:28 -0400 (EDT) 23, 39 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHOS31.ornl.gov 23, 39 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.775.38; Mon, 16 Jun 23, 39 -- 2014 07:59:28 -0400 23, 39 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by 23, 39 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id 23, 39 -- 15.00.0775.031; Mon, 16 Jun 2014 07:59:27 -0400 23, 39 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 23, 39 -- To: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu} 23, 39 -- CC: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 23, 39 -- Subject: RE: [Microscopy] spacial resolution of ZN 23, 39 -- Thread-Topic: [Microscopy] spacial resolution of ZN 23, 39 -- Thread-Index: AQHPh2bBjTTDBlclC0qlDo2QMwwHoJtzoxeA 23, 39 -- Date: Mon, 16 Jun 2014 11:59:26 +0000 23, 39 -- Message-ID: {41fe3e3478504beeb240292d0518000b-at-EXCHCS32.ornl.gov} 23, 39 -- References: {201406140022.s5E0M7to015409-at-ns.microscopy.com} 23, 39 -- In-Reply-To: {201406140022.s5E0M7to015409-at-ns.microscopy.com} 23, 39 -- Accept-Language: en-US 23, 39 -- Content-Language: en-US 23, 39 -- X-MS-Has-Attach: 23, 39 -- X-MS-TNEF-Correlator: 23, 39 -- x-originating-ip: [128.219.12.132] 23, 39 -- Content-Type: text/plain; charset="us-ascii" 23, 39 -- MIME-Version: 1.0 23, 39 -- Content-Transfer-Encoding: 8bit 23, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5GBxTsv006630 ==============================End of - Headers==============================
} I am not sure that TEM-EDS is the way to go or if another technique, } such as AFM, would work better to confirm the presence and } distribution of the Zn on the CNFs.
This looks to be an ideal application for the atom probe tomography. Samples are already forming sharp tips so not much sample preparation would be necessary - just their lift-out (which could be still difficult). But it might be more difficult to access such machine and its a question how it would be able to analyze this heterogeneous sample, a combination of metal and organic material. Have a nice day, Tomas
-- Tomas Hrncir, Ph.D. R&D - Physics http://www.tescan.cz TESCAN Brno +420 530353468 | tomas.hrncir-at-tescan.cz Libusina trida 1 | 623 00 Brno | Czech Republic
==============================Original Headers============================== 3, 21 -- From tomas.hrncir-at-tescan.cz Mon Jun 16 08:41:19 2014 3, 21 -- Received: from mail.tescan.cz (mail.tescan.cz [94.113.240.226]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5GDfIDa027572 3, 21 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jun 2014 08:41:19 -0500 3, 21 -- Received: from pctomas (pctomas.tescan2 [172.16.2.74]) 3, 21 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 3, 21 -- (No client certificate requested) 3, 21 -- by mail.tescan.cz (Postfix) with ESMTPSA id 024D61C01AD 3, 21 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jun 2014 15:41:18 +0200 (CEST) 3, 21 -- Date: Mon, 16 Jun 2014 15:41:26 +0200 3, 21 -- From: Tomas Hrncir {tomas.hrncir-at-tescan.cz} 3, 21 -- To: microscopy-at-microscopy.com 3, 21 -- Subject: Re: [Microscopy] spacial resolution of ZN 3, 21 -- Message-Id: {20140616154126.6f92a612d2452c66ce4f9107-at-tescan.cz} 3, 21 -- In-Reply-To: {201406140022.s5E0MdCt015512-at-ns.microscopy.com} 3, 21 -- References: {201406140022.s5E0MdCt015512-at-ns.microscopy.com} 3, 21 -- Organization: TESCAN 3, 21 -- X-Mailer: Sylpheed 3.4.0beta7 (GTK+ 2.10.14; i686-pc-mingw32) 3, 21 -- Mime-Version: 1.0 3, 21 -- Content-Type: text/plain; charset=US-ASCII 3, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You have an additional problem with ZnO, in addition to what others have said.
ZnO nanoparticles are very electron beam sensitive. My experience is that this material damages and sputters quickly over the entire range of 80-300 kV. You will likely need low dose techniques which are not really useful during XEDS analysis.
Nestor Your Friendly Neighborhood SysOp
On 6/13/14, 7:03 PM, dsherman-at-purdue.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } } I was asked if it would be possible to use TEM-EDS to map locations of } Zinc ions incorporated into cellulose nano fibers (CNF). These fibers can } be quite long but are often less than 20nm in width. At this point I have } no idea as to the amount of zinc that could be incorporated into the } fibers or the distribution. Does anyone have an idea as to the spacial } resolution limits of mapping nano-scale ZnO on CNFs? } } I am not sure that TEM-EDS is the way to go or if another technique, such } as AFM, would work better to confirm the presence and distribution of the } Zn on the CNFs. } } Debby } } Debra Sherman, Founder & CSO } DS imaging LLC } Purdue Technology Center } 1281 Win Hentschel Blvd } West Lafayette, IN 47906 } E-mail: debby.sherman-at-dsimagingllc.com } www.dsimagingllc.com } Mobile: 765-418-8540 } } } } ==============================Original Headers============================== } 7, 31 -- From dsherman-at-purdue.edu Fri Jun 13 19:02:59 2014 } 7, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) } 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5E02wjQ003265 } 7, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 19:02:58 -0500 } 7, 31 -- Received: from WPVEXCHUB04.purdue.lcl (wpvexchub04.itap.purdue.edu [172.30.136.70]) } 7, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s5E02wr0014408 } 7, 31 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2014 20:02:58 -0400 } 7, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([169.254.2.206]) by } 7, 31 -- WPVEXCHUB04.purdue.lcl ([172.30.136.70]) with mapi id 14.03.0174.001; Fri, 13 } 7, 31 -- Jun 2014 20:02:58 -0400 } 7, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} } 7, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 7, 31 -- Subject: spacial resolution of ZN } 7, 31 -- Thread-Topic: spacial resolution of ZN } 7, 31 -- Thread-Index: AQHPh2P/nK/2QiJHi0qkNxxx21dc8g== } 7, 31 -- Date: Sat, 14 Jun 2014 00:02:57 +0000 } 7, 31 -- Message-ID: {CFC1096F.1EEDE%dsherman-at-purdue.edu} } 7, 31 -- Accept-Language: en-US } 7, 31 -- Content-Language: en-US } 7, 31 -- X-MS-Has-Attach: } 7, 31 -- X-MS-TNEF-Correlator: } 7, 31 -- user-agent: Microsoft-MacOutlook/14.4.2.140509 } 7, 31 -- x-originating-ip: [24.14.170.252] } 7, 31 -- Content-Type: text/plain; charset="us-ascii" } 7, 31 -- Content-ID: {200F6AED770E76459E61997C221F8E94-at-exchange.purdue.edu} } 7, 31 -- MIME-Version: 1.0 } 7, 31 -- X-PMX-Version: 6.0.2.2308539 } 7, 31 -- X-PerlMx-URL-Scanned: Yes } 7, 31 -- X-PerlMx-Virus-Scanned: Yes } 7, 31 -- Content-Transfer-Encoding: 8bit } 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5E02wjQ003265 } ==============================End of - Headers============================== }
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Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Bldg 212 / A-143 9700 S. Cass Ave Argonne, Illinois 60439 USA
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
The box said ... "This program requires Win 95/98/NT or better..." So I bought a Mac !
===========================================
==============================Original Headers============================== 17, 26 -- From zaluzec-at-aaem.amc.anl.gov Mon Jun 16 12:45:43 2014 17, 26 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5GHjhh5017897 17, 26 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jun 2014 12:45:43 -0500 17, 26 -- Received: from localhost (localhost [127.0.0.1]) 17, 26 -- by aaem.amc.anl.gov (Postfix) with ESMTP id 11C58B4F83D 17, 26 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jun 2014 12:45:43 -0500 (CDT) 17, 26 -- X-Virus-Scanned: amavisd-new at aaem.amc.anl.gov 17, 26 -- Received: from aaem.amc.anl.gov ([127.0.0.1]) 17, 26 -- by localhost (aem005.amc.anl.gov [127.0.0.1]) (amavisd-new, port 10024) 17, 26 -- with ESMTP id giP8KOaM9zaK for {microscopy-at-microscopy.com} ; 17, 26 -- Mon, 16 Jun 2014 12:45:40 -0500 (CDT) 17, 26 -- Received: from aem108.amc.anl.gov (aem108.amc.anl.gov [146.139.72.108]) 17, 26 -- by aaem.amc.anl.gov (Postfix) with ESMTPA id EF588B4F832 17, 26 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jun 2014 12:45:40 -0500 (CDT) 17, 26 -- Message-ID: {539F2D4E.4020909-at-aaem.amc.anl.gov} 17, 26 -- Date: Mon, 16 Jun 2014 12:45:50 -0500 17, 26 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 17, 26 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.5.0 17, 26 -- MIME-Version: 1.0 17, 26 -- To: microscopy-at-microscopy.com 17, 26 -- Subject: AEM of ZnO 17, 26 -- References: {201406140003.s5E031RN003272-at-ns.microscopy.com} 17, 26 -- In-Reply-To: {201406140003.s5E031RN003272-at-ns.microscopy.com} 17, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From drugs-popular10-at-byfly.by Mon Jun 16 12:55:18 2014 Return-Path: {drugs-popular10-at-byfly.by} Received: from mm-154-37-215-37.minskobl.dynamic.pppoe.byfly.by ([37.215.37.154]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5GHtEWA030705 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 16 Jun 2014 12:55:15 -0500 Message-Id: {201406161755.s5GHtEWA030705-at-ns.microscopy.com}
A postdoctoral position is available in professor Laurence Marks Group at Northwestern University. The successful candidate will use TEM and STEM to study metal corrosion and oxidation. Expertise in (scanning) transmission electron microscopy is required, and familiarity with aberration corrected electron microscopy, metallurgy and/or electrochemistry is preferred. Interested candidates may send inquiries/CV to professor Laurence Marks (l-marks-at-northwestern.edu ) or Dr. Yifeng Liao (Yifeng.Liao-at-gmail.com).
Regards,
Yifeng Liao Northwestern University
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Email: de.wood-at-ars.usda.gov Name: Delilah Wood
Organization: USDA ARS
Title-Subject: [Filtered] X-Cite LED light source
Message: Has anyone tried the "new" LED light source for fluorescence microscopy? How does it compare to the metal halide type light source?
Thanks!
De Wood
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From medications_affordable1-at-bezeqint.net Tue Jun 17 13:51:24 2014 Return-Path: {medications_affordable1-at-bezeqint.net} Received: from bzq-79-179-212-198.red.bezeqint.net (bzq-79-179-212-198.red.bezeqint.net [79.179.212.198]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5HIpL7p022344 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 17 Jun 2014 13:51:22 -0500 Message-Id: {201406171851.s5HIpL7p022344-at-ns.microscopy.com}
A postodocoral position on Image Processing for 3D reconstruction and correlative analysis of light and electron microscopic data sets (CLEM) is available at the Sars International Centre for Marine Molecular Biology in Bergen, Norway.
see http://sars.no/jobs/postdoc_hausen14Sars_01.php
regards,
--
Harald Hausen, PhD Sars International Centre for Marine Molecular Biology Thormřhlensgate 55 NO-5008 Bergen Norway
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Email: vau-at-ufl.edu Name: Karen Kelley
Organization: University of Florida
Title-Subject: [Filtered] HPF/FS considered cryo?
Message: Is HPF/FS also considered a cryo-EM technique? Or it is only cryo when you do everything with frozen sample?
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Email: ecdickey-at-ncsu.edu Name: Elizabeth Dickey
Organization: North Carolina State University
Title-Subject: [Filtered] Postdoctoral Research Position
Message: A postdoctoral research position is available beginning as soon as July 1, 2014.
The position will support a major research initiative aimed at understanding the transport behavior and temporal evolution of interfaces in non-linear dielectrics as a function of electric-field biasing. The research program aims to develop experimental methodologies, based on novel ex-situ and in-situ structural, thermal, and electrical characterization techniques, to investigate spatial and temporal phenomena that control polarization switching, leakage current, degradation, and electrical breakdown in and around interfaces in non-linear dielectrics. The research program is coordinated with other experimental and theoretical groups, both in the US and internationally.
The postdoctoral scholar will provide leadership in experimental efforts aimed at studying the evolution of interface stoichiometry at metal-dielectric interfaces and understanding the implications for electrical transport. The research will focus on ex-situ and in-situ electron microscopy and electrical property studies, and the postdoctoral scholar will coordinate these efforts with the partnering institutions. The research will make extensive use of the aberration-corrected FEI TitanTM G2 scanning transmission electron microscope and other analytical equipment within the NCSU Analytical Instrumentation Facility. Results from the research program will be disseminated at international scientific conferences and in high-impact journals.
The ideal candidate will have knowledge of dielectric and ferroelectric materials, semiconductor physics, and have experience in scanning transmission electron microscopy.
Please apply to this position via: https://jobs.ncsu.edu/postings/38291
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Jun 18 07:22:42 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5ICMgGH009173 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2014 07:22:42 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id C956A71A806 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2014 07:22:42 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id kjWjRyMYYMEB for {microscopy-at-microscopy.com} ; 16, 28 -- Wed, 18 Jun 2014 07:22:37 -0500 (CDT) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 94B3B71A7F4 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2014 07:22:37 -0500 (CDT) 16, 28 -- Message-ID: {53A1848D.9070600-at-microscopy.com} 16, 28 -- Date: Wed, 18 Jun 2014 07:22:37 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:Postdoctoral Research Position NCSU 16, 28 -- References: {201406172227.s5HMROo5028832-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201406172227.s5HMROo5028832-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201406172227.s5HMROo5028832-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Karen, Cryo-EM is considered quick or plunge freezing of proteins, virus, molecules and viewing in the frozen vitrified (no ice crystals) state. These usually have no stains so EM tomography is used after digital image capture to reconstruct the samples 3D structure (need analysis software and some algorithms ie. FFT). HPF/FS is where live cells and tissues are frozen under high pressure to avoid ice crystal formation/damage. This frozen state should retain cellular structure as it was in the living state with no chemical fixation artifacts (shrinkage, local pH changes, selective fixation etc). AFS which follows HPF, allows for chemical cross-linking/stabilization at low temperatures, which does not show room temp fixation artifacts. The idea is that under low temps (-90C) the solvents in the AFS cocktails will substitute for the frozen water in the sample and also carry in the fixatives. Once in place these fixatives then work at about -50C to -60C which is artifact free to the sample (compared to ambient or cold fixation). Cryo-ultramicrotomy is where samples are frozen and sectioned in this state for immuno-electron microscopy labelling. All three techniques work on the basis of low temperature stabilization (liquid nitrogen temps) of biomolecules in the living state without ice crystal damage, chemical fixation artifacts or extraction.
Sincerely, Michael Delannoy Assoc. Director Johns Hopkins SOM Microscope Facility
Email: vau-at-ufl.edu Name: Karen Kelley
Organization: University of Florida
Title-Subject: [Filtered] HPF/FS considered cryo?
Message: Is HPF/FS also considered a cryo-EM technique? Or it is only cryo when you do everything with frozen sample?
==============================Original Headers============================== 9, 20 -- From prvs=239394d8c=mdelann1-at-jhmi.edu Wed Jun 18 08:37:34 2014 9, 20 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [128.220.160.200]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5IDbYYL015356 9, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2014 08:37:34 -0500 9, 20 -- X-IronPort-AV: E=Sophos;i="5.01,501,1400040000"; 9, 20 -- d="scan'208";a="489014657" 9, 20 -- Received: from bsno8925.ebalto.jhmi.edu (HELO BSNO8925) ([10.16.66.55]) 9, 20 -- by ipex3.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 18 Jun 2014 09:37:33 -0400 9, 20 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 9, 20 -- To: {Microscopy-at-microscopy.com} 9, 20 -- Subject: HPF/FS considered cryo? 9, 20 -- Date: Wed, 18 Jun 2014 09:37:52 -0400 9, 20 -- Message-ID: {001d01cf8afa$80d05df0$827119d0$-at-jhmi.edu} 9, 20 -- MIME-Version: 1.0 9, 20 -- Content-Type: text/plain; 9, 20 -- charset="us-ascii" 9, 20 -- Content-Transfer-Encoding: 7bit 9, 20 -- X-Mailer: Microsoft Outlook 14.0 9, 20 -- Thread-index: Ac+K+oCnESKjUNb3Sc2pSndx/TWSVg== 9, 20 -- Content-Language: en-us ==============================End of - Headers==============================
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Email: 12hy1-at-queensu.ca Name: Hongbing Yu
Organization: Queen's University
Title-Subject: [Filtered] Communicating problem of hot stage and computer
Message: Dear all, We have a Tecnai Osiris TEM. We purchased a Gatan Model 652 Double Tilt Heating Holder as well. But when we tilt the hot stage with the keyboard connected to the computer, the beta tilt can not properly control the holder, i.e, while the tilt angel displayed in the computer is 1 degree for beta tilt, the actual tilt displayed in the hot stage controller is only 0.1 degree. Even when we tilt using the controller of the hot stage, the computer can not give the right display either. Has anyone met this problem before or anyone can gives up a suggestion to figure out the problem? I appreciate any reply. Kind regard Hongbing PhD student Queen's University 60 Union Street Kingston,ON, K7L 3N6 Canada
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==============================Original Headers============================== 13, 29 -- From microscopylistserver-noreply-at-microscopy.com Wed Jun 18 20:41:42 2014 13, 29 -- Received: from znl.com ([206.69.208.20]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5J1fgS2024729 13, 29 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2014 20:41:42 -0500 13, 29 -- Received: from localhost (localhost [127.0.0.1]) 13, 29 -- by znl.com (Postfix) with ESMTP id 5A11671AE41 13, 29 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2014 20:41:42 -0500 (CDT) 13, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 29 -- Received: from znl.com ([127.0.0.1]) 13, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 29 -- with ESMTP id TkNVglQu8xmT for {microscopy-at-microscopy.com} ; 13, 29 -- Wed, 18 Jun 2014 20:41:38 -0500 (CDT) 13, 29 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 29 -- by znl.com (Postfix) with ESMTPA id D612D71AE34 13, 29 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2014 20:41:38 -0500 (CDT) 13, 29 -- Message-ID: {53A23FD2.8030009-at-microscopy.com} 13, 29 -- Date: Wed, 18 Jun 2014 20:41:38 -0500 13, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 29 -- MIME-Version: 1.0 13, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 29 -- Subject: viaWWW:Digital Micrograph Scripting School at 18th International 13, 29 -- Microscopy Congress 13, 29 -- References: {201406182208.s5IM8EHn021545-at-ns.microscopy.com} 13, 29 -- In-Reply-To: {201406182208.s5IM8EHn021545-at-ns.microscopy.com} 13, 29 -- X-Forwarded-Message-Id: {201406182208.s5IM8EHn021545-at-ns.microscopy.com} 13, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From canadian-pharmacy1-at-ketterers.net Thu Jun 19 07:00:58 2014 Return-Path: {canadian-pharmacy1-at-ketterers.net} Received: from mail4.ketterers.net ([176.51.241.243]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5JC0uBw006296 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 19 Jun 2014 07:00:57 -0500 Message-Id: {201406191200.s5JC0uBw006296-at-ns.microscopy.com}
Dear Colleagues,
We would like to invite you to submit abstracts to the MRS Fall 2014 Symposium PP Advances in “Scanning Probe Microscopy for Multimodal Imaging at the Nanoscale”. The symposium will cover research aimed at understanding the spatially distinct chemical and physical makeup and dynamic processes ongoing at solid-solid, solid-liquid, and solid-gas interfaces is central to accelerating major advances in areas including energy, national security and microbiology research. Please submit your abstracts though the MRS homepage by today (EST 11:59 pm, June 19, 2014).
http://www.mrs.org/fall2014/
Thank You!
==============================Original Headers============================== 6, 23 -- From sergei2-at-ornl.gov Thu Jun 19 10:26:29 2014 6, 23 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5JFQTw4009917 6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jun 2014 10:26:29 -0500 6, 23 -- X-SG: RELAYLIST 6, 23 -- X-IronPort-AV: E=Sophos;i="5.01,507,1400040000"; 6, 23 -- d="scan'208";a="59355271" 6, 23 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 6, 23 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 19 Jun 2014 11:26:28 -0400 6, 23 -- Received: from [128.219.192.92] (pc83680.ornl.gov [128.219.192.92]) 6, 23 -- (using TLSv1 with cipher DHE-RSA-CAMELLIA256-SHA (256/256 bits)) 6, 23 -- (No client certificate requested) 6, 23 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id BF5981006F6 6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jun 2014 11:26:28 -0400 (EDT) 6, 23 -- Message-ID: {53A30124.6010104-at-ornl.gov} 6, 23 -- Date: Thu, 19 Jun 2014 11:26:28 -0400 6, 23 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 6, 23 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:14.0) Gecko/20120713 Thunderbird/14.0 6, 23 -- MIME-Version: 1.0 6, 23 -- To: microscopy-at-microscopy.com 6, 23 -- Subject: MRS Fall Symposium on Scanning Probe Microscopies 6, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 23 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
A little more clarity, The EM tomography is done on the scope with a series of tilt angles over different fields. These tomographs are then analysed with software to get 3D structures.
Sincerely, Michael Delannoy
==============================Original Headers============================== 3, 20 -- From prvs=240c3b11e=mdelann1-at-jhmi.edu Thu Jun 19 12:32:49 2014 3, 20 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [128.220.161.23]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5JHWn83030394 3, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Jun 2014 12:32:49 -0500 3, 20 -- X-IronPort-AV: E=Sophos;i="5.01,508,1400040000"; 3, 20 -- d="scan'208";a="72305847" 3, 20 -- Received: from bsno8925.ebalto.jhmi.edu (HELO BSNO8925) ([10.16.66.55]) 3, 20 -- by IPEX5.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 19 Jun 2014 13:32:24 -0400 3, 20 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 3, 20 -- To: {Microscopy-at-microscopy.com} 3, 20 -- Subject: HPF/FS considered cryo? 3, 20 -- Date: Thu, 19 Jun 2014 13:32:42 -0400 3, 20 -- Message-ID: {000501cf8be4$799eac40$6cdc04c0$-at-jhmi.edu} 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: text/plain; 3, 20 -- charset="us-ascii" 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-Mailer: Microsoft Outlook 14.0 3, 20 -- Thread-Index: Ac+L5DH+CCMx22ovR0ahg/LYd+z2fQ== 3, 20 -- Content-Language: en-us ==============================End of - Headers==============================
From noreply-at-systemhat.com Thu Jun 19 13:30:47 2014 Return-Path: {noreply-at-systemhat.com} Received: from ip28.hichina.com (ip28.hichina.com [115.28.0.169] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id s5JIUj6S017858 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 19 Jun 2014 13:30:46 -0500 Received: from mail99c7.megamailservers.com (mail99c7.megamailservers.com [178.255.240.10]) by ip28.hichina.com with ESMTP id 2L50HP0LT2VN312 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 19 Jun 2014 13:07:48 -0600 Received: from localhost (localhost.localdomain [127.0.0.1]) by mail99c7.megamailservers.com via ESMTP id ED8CJ1U84SLF0SL for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 19 Jun 2014 13:05:40 -0600 Message-ID: {930FAE63EBC0F6CFBBED2D100AFE9BED31F8BBC-at-systemhat.com}
Dear Karen,
I've worked on both single particle cryoEM, cryo tomography and HPF/FS. While the FS part (in my honest opinion) is kind of borderline cryo, I think HPF is indeed a cryo technique. Just like you plunge-freeze things into a liquid ethane or propane, you sort of do the same in HPF, except the sample is thicker. But even after freezing the sample, you quickly move it to a container where the hats/planchettes can be separated that is kept at LN2 temperature. I do count it as a cryo technique. Depending on what is done in the end with the samples (whether looked at with a FIB-SEM in a cryo stage) or cryo ultramicrotomy, or FS and microtomy. Perhaps you should ask Kent McDonald for his two cents.
Hope this helps,
Mariena
Mariena Silvestry Ramos, PhD Senior Research Specialist Director of Operations, CryoEM Facility Center for Structural Biology Vanderbilt University Medical Center North, Suite RR1207 Nashville, TN 37232-8325 ph. 615-322-4671
________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, June 18, 2014 7:42 AM To: Silvestry Ramos, Mariena O
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Email: vau-at-ufl.edu Name: Karen Kelley
Organization: University of Florida
Title-Subject: [Filtered] HPF/FS considered cryo?
Message: Is HPF/FS also considered a cryo-EM technique? Or it is only cryo when you do everything with frozen sample?
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==============================Original Headers============================== 24, 46 -- From mariena.silvestry-at-vanderbilt.edu Thu Jun 19 14:04:37 2014 24, 46 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1ehsobe004.messaging.microsoft.com [216.32.181.184]) 24, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5JJ4bxT018245 24, 46 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Jun 2014 14:04:37 -0500 24, 46 -- Received: from mail35-ch1-R.bigfish.com (10.43.68.252) by 24, 46 -- CH1EHSOBE005.bigfish.com (10.43.70.55) with Microsoft SMTP Server id 24, 46 -- 14.1.225.22; Thu, 19 Jun 2014 19:04:36 +0000 24, 46 -- Received: from mail35-ch1 (localhost [127.0.0.1]) by mail35-ch1-R.bigfish.com 24, 46 -- (Postfix) with ESMTP id 9D3D9220378; Thu, 19 Jun 2014 19:04:36 +0000 (UTC) 24, 46 -- X-Forefront-Antispam-Report: CIP:129.59.94.73;KIP:(null);UIP:(null);IPV:NLI;H:hub.vanderbilt.edu;RD:error;EFVD:FOP 24, 46 -- X-SpamScore: -1 24, 46 -- X-BigFish: VPS-1(zz62a3I9371I1936Ie0eah328cMzz1f42h1d77h1ee6h1de0h1fdah2073h2146h1202h1e76h2189h1d1ah1d2ah21bch2297h1fc6h208chzd9hz1de098h17326ah8275bh1de097h186068hz2dh109h2a8h839h944hd25hf0ah1220h1288h12a5h12a9h12bdh137ah13b6h1441h1504h1537h153bh15d0h162dh1631h1758h18e1h1946h19b5h19ceh1ad9h1b0ah1b2fh2222h224fh1fb3h1d0ch1d2eh1d3fh1dfeh1dffh1fe8h1ff5h2216h22d0h2336h2438h2461h2487h24d7h2516h2545h255eh25f6h2605h262fh268bh26d3h27e2h28a1h2973h1b1cn1b1bi) 24, 46 -- Received: from mail35-ch1 (localhost.localdomain [127.0.0.1]) by mail35-ch1 24, 46 -- (MessageSwitch) id 1403204674734230_13461; Thu, 19 Jun 2014 19:04:34 +0000 24, 46 -- (UTC) 24, 46 -- Received: from CH1EHSMHS025.bigfish.com (snatpool2.int.messaging.microsoft.com 24, 46 -- [10.43.68.237]) by mail35-ch1.bigfish.com (Postfix) with ESMTP id 24, 46 -- A6A3D32004E; Thu, 19 Jun 2014 19:04:34 +0000 (UTC) 24, 46 -- Received: from hub.vanderbilt.edu (129.59.94.73) by CH1EHSMHS025.bigfish.com 24, 46 -- (10.43.70.25) with Microsoft SMTP Server (TLS) id 14.16.227.3; Thu, 19 Jun 24, 46 -- 2014 19:04:33 +0000 24, 46 -- Received: from ITS-HCWNEM106.ds.vanderbilt.edu ([10.1.154.89]) by 24, 46 -- ITS-HCWNEM122.ds.vanderbilt.edu ([::1]) with mapi id 14.03.0174.001; Thu, 19 24, 46 -- Jun 2014 14:04:08 -0500 24, 46 -- From: "Silvestry Ramos, Mariena O" {mariena.silvestry-at-vanderbilt.edu} 24, 46 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , 24, 46 -- "vau-at-ufl.edu" 24, 46 -- {vau-at-ufl.edu} 24, 46 -- Subject: RE: [Microscopy] viaWWW:HPF/FS considered cryo? 24, 46 -- Thread-Topic: [Microscopy] viaWWW:HPF/FS considered cryo? 24, 46 -- Thread-Index: AQHPivLT5H91hwkrAUmIO74rbNaRvZt4y9LE 24, 46 -- Date: Thu, 19 Jun 2014 19:04:06 +0000 24, 46 -- Message-ID: {A7FBE81A1EC75E47885645516BF3A43304F5A031-at-ITS-HCWNEM106.ds.vanderbilt.edu} 24, 46 -- References: {201406181242.s5ICgmbs010079-at-ns.microscopy.com} 24, 46 -- In-Reply-To: {201406181242.s5ICgmbs010079-at-ns.microscopy.com} 24, 46 -- Accept-Language: en-US 24, 46 -- Content-Language: en-US 24, 46 -- X-MS-Has-Attach: 24, 46 -- X-MS-TNEF-Correlator: 24, 46 -- x-originating-ip: [129.59.15.136] 24, 46 -- Content-Type: text/plain; charset="us-ascii" 24, 46 -- MIME-Version: 1.0 24, 46 -- X-OriginatorOrg: vanderbilt.edu 24, 46 -- X-FOPE-CONNECTOR: Id%0$Dn%*$RO%0$TLS%0$FQDN%$TlsDn% 24, 46 -- Content-Transfer-Encoding: 8bit 24, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5JJ4bxT018245 ==============================End of - Headers==============================
} Is HPF/FS also considered a cryo-EM technique? Or } it is only cryo when you do everything with frozen sample?
there is some consensus on the following terminology:
* Cryo-TEM = Microscopy of frozen hydrated specimens at low temperature (including, but not necessarily electron tomography) that were prepared by immersion freezing, high pressure freezing, etc.
As HPF/FS specimens are being inspected at room temperature in the TEM, this is NOT cryo-TEM, but a cryo-preparation technique for TEM. The same applies, for example, to Tokuyasu cryo-sections that are later thawed for immunolabelling.
Hope that helps,
Guenter
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
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From drugs_express9-at-reelinfish.com Fri Jun 20 03:25:52 2014 Return-Path: {drugs_express9-at-reelinfish.com} Received: from mail2.reelinfish.com ([46.46.147.50]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5K8PoZZ031606 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 20 Jun 2014 03:25:50 -0500 Message-Id: {201406200825.s5K8PoZZ031606-at-ns.microscopy.com}
Researchers from another campus want to be able to prepare TEM specimens of their nanoparticles by themselves and are asking for a protocol. I want to make things a bit easier for them by substituting uranyl acetate with phosphotungstic acid for negative staining. Can something go wrong because of this replacement?
Thanks,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
==============================Original Headers============================== 5, 28 -- From DusevichV-at-umkc.edu Fri Jun 20 14:15:07 2014 5, 28 -- Received: from mst-rip5-umkc-out.um.umsystem.edu (mst-rip5-umkc-out.um.umsystem.edu [198.209.50.138]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5KJF6QY006912 5, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jun 2014 14:15:07 -0500 5, 28 -- X-IronPort-Anti-Spam-Filtered: true 5, 28 -- X-IronPort-Anti-Spam-Result: AhMFAE6HpFPPoJ7E/2dsb2JhbABWA4MNgSzAQoMUAYENFnWEBQU6UQEqFEImAQQbiDqcZaduhXMXjkVJgxyBFgSuHoNCgjA 5, 28 -- X-IPAS-Result: AhMFAE6HpFPPoJ7E/2dsb2JhbABWA4MNgSzAQoMUAYENFnWEBQU6UQEqFEImAQQbiDqcZaduhXMXjkVJgxyBFgSuHoNCgjA 5, 28 -- Received: from um-tcas1.um.umsystem.edu ([207.160.158.196]) 5, 28 -- by mst-rip5-exch-relay.um.umsystem.edu with ESMTP; 20 Jun 2014 14:15:06 -0500 5, 28 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.129]) by 5, 28 -- UM-TCAS1.um.umsystem.edu ([207.160.158.196]) with mapi id 14.03.0181.006; 5, 28 -- Fri, 20 Jun 2014 14:15:05 -0500 5, 28 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 5, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 28 -- Subject: PTA vs UA for negative staining 5, 28 -- Thread-Topic: PTA vs UA for negative staining 5, 28 -- Thread-Index: Ac+Mu+Dxj1/+xafmSWaBQTUSUVaZBg== 5, 28 -- Date: Fri, 20 Jun 2014 19:15:05 +0000 5, 28 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DBD7C3F-at-UM-MBX-T01.um.umsystem.edu} 5, 28 -- Accept-Language: en-US 5, 28 -- Content-Language: en-US 5, 28 -- X-MS-Has-Attach: 5, 28 -- X-MS-TNEF-Correlator: 5, 28 -- x-originating-ip: [134.193.156.218] 5, 28 -- Content-Type: text/plain; charset="us-ascii" 5, 28 -- MIME-Version: 1.0 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5KJF6QY006912 ==============================End of - Headers==============================
From drugs_express1-at-global-ip.net Sat Jun 21 02:54:11 2014 Return-Path: {drugs_express1-at-global-ip.net} Received: from mail4.global-ip.net ([95.71.51.33]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5L7s72l007477 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 21 Jun 2014 02:54:09 -0500 Message-Id: {201406210754.s5L7s72l007477-at-ns.microscopy.com}
Hi Vladimir,
} Researchers from another campus want to be able to prepare TEM specimens of their nanoparticles by themselves and are asking for a protocol. I want to make things a bit easier for them by substituting uranyl acetate with phosphotungstic acid for negative staining. Can something go wrong because of this replacement?
from my own experience with UA (acidic) and PTA (neutral; usually both at 2.0%) for negative staining of viruses and macromolecular complexes, I have observed differences in stain distribution, resolution and - interesting enough - also the optimum concentration of the specimen. UA typically requires a less concentrated sample for optimal results.
Having said that, both stains can produce very nice results, but a switch from one to the other might require adjustment of some parameters in the protocol.
Best regards,
Guenter
P.S: Do these NPs actually need to be stained? Depending on the material/size/question at hand you might try to dry them down on a C film, with or without washing.
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
==============================Original Headers============================== 8, 25 -- From lists-at-nexperion.net Sat Jun 21 10:32:46 2014 8, 25 -- Received: from 503.hosttech.eu (503.hosttech.eu [62.112.145.5]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5LFWjks006828 8, 25 -- for {Microscopy-at-microscopy.com} ; Sat, 21 Jun 2014 10:32:45 -0500 8, 25 -- Received: from localhost (unknown [127.0.0.1]) 8, 25 -- by 503.hosttech.eu (Postfix) with ESMTP id 096746C0C2F; 8, 25 -- Sat, 21 Jun 2014 15:32:44 +0000 (UTC) 8, 25 -- X-Virus-Scanned: amavisd-new at example.com 8, 25 -- Received: from 503.hosttech.eu ([127.0.0.1]) 8, 25 -- by localhost (503.hosttech.eu [127.0.0.1]) (amavisd-new, port 10024) 8, 25 -- with ESMTP id MzTGgg2o6Lsq; Sat, 21 Jun 2014 17:32:37 +0200 (CEST) 8, 25 -- Received: from aventine (chello080108026214.8.11.vie.surfer.at [80.108.26.214]) 8, 25 -- by 503.hosttech.eu (Postfix) with ESMTPSA id 05E4D6C0C2E; 8, 25 -- Sat, 21 Jun 2014 17:32:36 +0200 (CEST) 8, 25 -- Date: Sat, 21 Jun 2014 17:32:36 +0200 8, 25 -- From: Guenter Resch {lists-at-nexperion.net} 8, 25 -- To: DusevichV-at-umkc.edu, Microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] PTA vs UA for negative staining 8, 25 -- Message-ID: {20140621173236.75859bb9-at-aventine} 8, 25 -- In-Reply-To: {201406201944.s5KJikb4024723-at-ns.microscopy.com} 8, 25 -- References: {201406201944.s5KJikb4024723-at-ns.microscopy.com} 8, 25 -- X-Mailer: Claws Mail 3.9.3 (GTK+ 2.24.23; x86_64-pc-linux-gnu) 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; charset=US-ASCII 8, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From medications-express7-at-visualinvestments.com Sun Jun 22 01:55:47 2014 Return-Path: {medications-express7-at-visualinvestments.com} Received: from mail3.visualinvestments.com ([31.129.111.72]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5M6ticc019322 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 22 Jun 2014 01:55:44 -0500 Message-Id: {201406220655.s5M6ticc019322-at-ns.microscopy.com}
Hello everybody! I thought that the 2d-FFT of a HRTEM image taken in MAG mode of my TEM equals the diffraction pattern which I also could have taken in DIFF mode of the same sample area. Right? Well, if I acquire the diffractogram in DIFF mode, then I know which camera length I have had in use. But what´s about the 2d-FFT which was calculated from a real space image? How do I know which "camera length" is represented in there? I am asking, because I would like to save a 2d-FFT as an image and underlay it to a calculated powder diffraction pattern. But in order to scale my 2d-FFT "image" and the powder diffraction ring pattern "image" appropriately, the camera length would have to be known and I am missing how to derive that value. Thanks, Marco
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==============================Original Headers============================== 6, 34 -- From mmoller-at-cicbiomagune.es Sun Jun 22 07:20:34 2014 6, 34 -- Received: from mail.cicbiomagune.es (mail.cicbiomagune.es [150.241.252.35]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5MCKWeG003978 6, 34 -- for {Microscopy-at-Microscopy.Com} ; Sun, 22 Jun 2014 07:20:32 -0500 6, 34 -- X-AuditID: 0a0d0165-f79206d000001000-ad-53a6ca0fc3d7 6, 34 -- Received: from CICMGE007.cicbiomagune.int (cicmge009.cicbiomagune.int [10.13.1.109]) 6, 34 -- by mail.cicbiomagune.es (Symantec Messaging Gateway) with SMTP id EA.E1.04096.F0AC6A35; Sun, 22 Jun 2014 14:20:31 +0200 (CEST) 6, 34 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4913 6, 34 -- Content-Class: urn:content-classes:message 6, 34 -- MIME-Version: 1.0 6, 34 -- Content-Type: text/plain; 6, 34 -- charset="iso-8859-1" 6, 34 -- Subject: "camera length" represented in the 2d-FFT of a HRTEM image? 6, 34 -- Date: Sun, 22 Jun 2014 14:20:30 +0200 6, 34 -- Message-ID: {0076FB6F2203944CAAA265CF412935E8BC5B06-at-CICMGE007.cicbiomagune.int} 6, 34 -- X-MS-Has-Attach: 6, 34 -- X-MS-TNEF-Correlator: 6, 34 -- Thread-Topic: "camera length" represented in the 2d-FFT of a HRTEM image? 6, 34 -- thread-index: Ac+OFFupbjzqO/loQJCmKLCdTlagaQ== 6, 34 -- From: "Marco Moller" {mmoller-at-cicbiomagune.es} 6, 34 -- To: {Microscopy-at-Microscopy.Com} 6, 34 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFjrCLMWRmVeSWpSXmKPExsXCxcuYq8t/almwwZe/OhZ/DjewOzB6fOx+ 6, 34 -- zRTAGMVlk5Kak1mWWqRvl8CVMeXqTpaC+VwVex69ZGlgnMjRxcjBISFgIrFxkksXIyeQKSZx 6, 34 -- 4d56ti5GLg4hgauMEr3HrrJA1FhIXP+RAFLDLKAlsWPCPkYQm1dAUOLkzCcsEHEDifuHOlgh 6, 34 -- bG2JZQtfM4PYwgIuEm9+9YPVsAioShybeYEdZCSvgL/E7NmSEGsFJRbN3sMMc8K/XQ/ZQGwR 6, 34 -- AU+JSWubWCFsPYmeaY/B1rIB2demXwe7TERAXmLWdcsJjIKzkBw3C8lxs5AcNwvJcQsYWVYx 6, 34 -- SicVZaZnlOQmZuboJWcmJ2Xm5yaml+al6gHDdxMjJGxTdzDun6h7iFGAg1GJh/e3yLJgIdbE 6, 34 -- suLK3EOMEhzMSiK8kU5AId6UxMqq1KL8+KLSnNTiQ4zSHCxK4rw+7y76CwmkJ5akZqemFqQW 6, 34 -- wWSZODilGhidAt6fbHixzHDf3nu/ejaFz4nl2v2k5ORi39jj5513hX4VqsmR2RVyS5Hty0HH 6, 34 -- M1JrT13Yu2z2rEn3OqYu9ooTcP1md6nsxO2wVwd7SnauYZFYt2+q7QNNhctP2Y5NLN2RKLbq 6, 34 -- dsULWQ3+UIt2xrknDm7LWNhq/zf5pfxjd+mavRMN+l6/SjrBp8RSnJFoqMVcVJwIAKpmH0VX 6, 34 -- AgAA 6, 34 -- Content-Transfer-Encoding: 8bit 6, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5MCKWeG003978 ==============================End of - Headers==============================
The FFT of the image isn't exactly like a diffraction pattern since the phase information has been lost in the image. It is more properly called a "power spectrum".
The "camera length" of the FFT is based on the image mag. The easiest way to determine this is to take the pixel calibration of the image and invert the value. So, if the image calibration is 2 pixels/nm or 0.5 nm/pixel, then the FFT will have a calibration of 2.0 nm-1 per pixel.
You can take your "powder" pattern, calculate the d-spacing of the rings and from that determine the calibration of your pattern in nm-1. At that pont, you just need to scale the two patterns identically.
Cheers, Henk
On 6/22/2014 8:25 AM, mmoller-at-cicbiomagune.es wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello everybody! } I thought that the 2d-FFT of a HRTEM image taken in MAG mode of my TEM equals the diffraction pattern which I also could have taken in DIFF mode of the same sample area. Right? } Well, if I acquire the diffractogram in DIFF mode, then I know which camera length I have had in use. But what´s about the 2d-FFT which was calculated from a real space image? How do I know which "camera length" is represented in there? } I am asking, because I would like to save a 2d-FFT as an image and underlay it to a calculated powder diffraction pattern. But in order to scale my 2d-FFT "image" and the powder diffraction ring pattern "image" appropriately, the camera length would have to be known and I am missing how to derive that value. } Thanks, } Marco } } } This e-mail is from CIC biomaGUNE. The e-mail and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any unauthorised dissemination or copying of this e-mail or its attachments, and any use or disclosure of any information contained in them, is strictly prohibited and may be illegal. If you have received this e-mail in error, please notify or telephone + 34 943 00 53 00 and delete it from your system. } } } } } ==============================Original Headers============================== } 6, 34 -- From mmoller-at-cicbiomagune.es Sun Jun 22 07:20:34 2014 } 6, 34 -- Received: from mail.cicbiomagune.es (mail.cicbiomagune.es [150.241.252.35]) } 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5MCKWeG003978 } 6, 34 -- for {Microscopy-at-Microscopy.Com} ; Sun, 22 Jun 2014 07:20:32 -0500 } 6, 34 -- X-AuditID: 0a0d0165-f79206d000001000-ad-53a6ca0fc3d7 } 6, 34 -- Received: from CICMGE007.cicbiomagune.int (cicmge009.cicbiomagune.int [10.13.1.109]) } 6, 34 -- by mail.cicbiomagune.es (Symantec Messaging Gateway) with SMTP id EA.E1.04096.F0AC6A35; Sun, 22 Jun 2014 14:20:31 +0200 (CEST) } 6, 34 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4913 } 6, 34 -- Content-Class: urn:content-classes:message } 6, 34 -- MIME-Version: 1.0 } 6, 34 -- Content-Type: text/plain; } 6, 34 -- charset="iso-8859-1" } 6, 34 -- Subject: "camera length" represented in the 2d-FFT of a HRTEM image? } 6, 34 -- Date: Sun, 22 Jun 2014 14:20:30 +0200 } 6, 34 -- Message-ID: {0076FB6F2203944CAAA265CF412935E8BC5B06-at-CICMGE007.cicbiomagune.int} } 6, 34 -- X-MS-Has-Attach: } 6, 34 -- X-MS-TNEF-Correlator: } 6, 34 -- Thread-Topic: "camera length" represented in the 2d-FFT of a HRTEM image? } 6, 34 -- thread-index: Ac+OFFupbjzqO/loQJCmKLCdTlagaQ== } 6, 34 -- From: "Marco Moller" {mmoller-at-cicbiomagune.es} } 6, 34 -- To: {Microscopy-at-Microscopy.Com} } 6, 34 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFjrCLMWRmVeSWpSXmKPExsXCxcuYq8t/almwwZe/OhZ/DjewOzB6fOx+ } 6, 34 -- zRTAGMVlk5Kak1mWWqRvl8CVMeXqTpaC+VwVex69ZGlgnMjRxcjBISFgIrFxkksXIyeQKSZx } 6, 34 -- 4d56ti5GLg4hgauMEr3HrrJA1FhIXP+RAFLDLKAlsWPCPkYQm1dAUOLkzCcsEHEDifuHOlgh } 6, 34 -- bG2JZQtfM4PYwgIuEm9+9YPVsAioShybeYEdZCSvgL/E7NmSEGsFJRbN3sMMc8K/XQ/ZQGwR } 6, 34 -- AU+JSWubWCFsPYmeaY/B1rIB2demXwe7TERAXmLWdcsJjIKzkBw3C8lxs5AcNwvJcQsYWVYx } 6, 34 -- SicVZaZnlOQmZuboJWcmJ2Xm5yaml+al6gHDdxMjJGxTdzDun6h7iFGAg1GJh/e3yLJgIdbE } 6, 34 -- suLK3EOMEhzMSiK8kU5AId6UxMqq1KL8+KLSnNTiQ4zSHCxK4rw+7y76CwmkJ5akZqemFqQW } 6, 34 -- wWSZODilGhidAt6fbHixzHDf3nu/ejaFz4nl2v2k5ORi39jj5513hX4VqsmR2RVyS5Hty0HH } 6, 34 -- M1JrT13Yu2z2rEn3OqYu9ooTcP1md6nsxO2wVwd7SnauYZFYt2+q7QNNhctP2Y5NLN2RKLbq } 6, 34 -- dsULWQ3+UIt2xrknDm7LWNhq/zf5pfxjd+mavRMN+l6/SjrBp8RSnJFoqMVcVJwIAKpmH0VX } 6, 34 -- AgAA } 6, 34 -- Content-Transfer-Encoding: 8bit } 6, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5MCKWeG003978 } ==============================End of - Headers============================== }
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
THANK YOU TO EVERYBODY FOR THE VERY VALUABLE INFORMATION! Marco
On 6/22/2014 8:25 AM, mmoller-at-cicbiomagune.es wrote: } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello everybody! } I thought that the 2d-FFT of a HRTEM image taken in MAG mode of my TEM equals the diffraction pattern which I also could have taken in DIFF mode of the same sample area. Right? } Well, if I acquire the diffractogram in DIFF mode, then I know which camera length I have had in use. But what´s about the 2d-FFT which was calculated from a real space image? How do I know which "camera length" is represented in there? } I am asking, because I would like to save a 2d-FFT as an image and underlay it to a calculated powder diffraction pattern. But in order to scale my 2d-FFT "image" and the powder diffraction ring pattern "image" appropriately, the camera length would have to be known and I am missing how to derive that value. } Thanks, } Marco
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==============================Original Headers============================== 9, 36 -- From mmoller-at-cicbiomagune.es Sun Jun 22 16:40:31 2014 9, 36 -- Received: from mail.cicbiomagune.es (mail.cicbiomagune.es [150.241.252.35]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5MLeUPr016172 9, 36 -- for {Microscopy-at-Microscopy.Com} ; Sun, 22 Jun 2014 16:40:31 -0500 9, 36 -- X-AuditID: 0a0d0165-f79206d000001000-f6-53a74d4ac219 9, 36 -- Received: from CICMGE007.cicbiomagune.int (cicmge009.cicbiomagune.int [10.13.1.109]) 9, 36 -- by mail.cicbiomagune.es (Symantec Messaging Gateway) with SMTP id DB.F1.04096.A4D47A35; Sun, 22 Jun 2014 23:40:26 +0200 (CEST) 9, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4913 9, 36 -- Content-Class: urn:content-classes:message 9, 36 -- MIME-Version: 1.0 9, 36 -- Content-Type: text/plain; 9, 36 -- charset="iso-8859-1" 9, 36 -- Subject: RE: [Microscopy] Re: "camera length" represented in the 2d-FFT of a HRTEM 9, 36 -- Date: Sun, 22 Jun 2014 23:40:25 +0200 9, 36 -- Message-ID: {0076FB6F2203944CAAA265CF412935E8BC5B0D-at-CICMGE007.cicbiomagune.int} 9, 36 -- In-Reply-To: {201406221755.s5MHtcFc008537-at-ns.microscopy.com} 9, 36 -- X-MS-Has-Attach: 9, 36 -- X-MS-TNEF-Correlator: 9, 36 -- Thread-Topic: [Microscopy] Re: "camera length" represented in the 2d-FFT of a HRTEM 9, 36 -- thread-index: Ac+OQy/TpC3pN8TaR5KKKWmZjQHjnQAHtEUg 9, 36 -- References: {201406221755.s5MHtcFc008537-at-ns.microscopy.com} 9, 36 -- From: "Marco Moller" {mmoller-at-cicbiomagune.es} 9, 36 -- To: {Microscopy-at-Microscopy.Com} 9, 36 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFrrLLMWRmVeSWpSXmKPExsXCxcuYq+vjuzzY4N52EYs/hxvYHRg9Pna/ 9, 36 -- ZgpgjOKySUnNySxLLdK3S+DKuLZkEnvBEb6K37MEGhhfcHcxcnJICJhIrPg8ixHCFpO4cG89 9, 36 -- WxcjF4eQwFVGiVtXO1i7GDmAEhYS138kgNQwC2hJ7JiwD6yeV0BQ4uTMJywQcQOJ+4dAykFs 9, 36 -- bYllC18zg9jCAkESq19sYQexWQRUJU7uWQzV6y+xqPsHWJxTwEbi/IJWdogbBCUWzd7DDHPP 9, 36 -- v10P2UBsEYFgiZuHDzJD2EYSM3o+MIHYQgLWEvsP/gSrYRPQk7g2/ToLyMkiAvISs65bTmAU 9, 36 -- noXk6llIrp6F5OpZSK5ewMiyilE6qSgzPaMkNzEzRy85MzkpMz83Mb00L1UPGPCbGCGBnrqD 9, 36 -- cf9E3UOMAhyMSjy8Gm7Lg4VYE8uKK3MPMUpwMCuJ8JYoAYV4UxIrq1KL8uOLSnNSiw8xSnOw 9, 36 -- KInz+ry76C8kkJ5YkpqdmlqQWgSTZeLglGpgLDrQGffmE/edzPnP/KLlO/MW9aTxn+90Kyys 9, 36 -- Kz+59i57aX7tV5Nr0z+d+TOny3qzzs3ke33e7e6H5a5qrlj+e1OY9I1qmwbLE0L2dYo5hlxv 9, 36 -- 73ZqFl1qEBL+nn3Q9dYD/trpqmx2Uz03C/9k0J3kbS66g7Fwh3qo2bNgAafjD/l8Xsukntql 9, 36 -- xFKckWioxVxUnAgAj9IURXACAAA= 9, 36 -- Content-Transfer-Encoding: 8bit 9, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5MLeUPr016172 ==============================End of - Headers==============================
From affordable-meds6-at-zaural.ru Mon Jun 23 00:50:33 2014 Return-Path: {affordable-meds6-at-zaural.ru} Received: from ws101.zone31-163-140.zaural.ru ([31.163.140.101]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5N5oSUF006392 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 23 Jun 2014 00:50:29 -0500 Message-Id: {201406230550.s5N5oSUF006392-at-ns.microscopy.com}
Hello Vladimir, I agree with Guenter that you do not need to negatively stain the NPs in some cases. However, if NPs are conjugated with some ligands or covered with organic layer(s), then the negatives staining is necessary. In this case, we are routinely using ammonium molybdate in concentration ranging from 0.1% to 1% in ddH2O. In our hands, both uranyl acetate and phosphotungstic acid are too electron opaque and can hide the NPs.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Sat, 21 Jun 2014 10:38:40 -0500, lists-at-nexperion.net wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Vladimir, } } } Researchers from another campus want to be able to prepare TEM } } specimens of their nanoparticles by themselves and are asking for a } } protocol. I want to make things a bit easier for them by } } substituting uranyl acetate with phosphotungstic acid for negative } } staining. Can something go wrong because of this replacement? } } from my own experience with UA (acidic) and PTA (neutral; usually } both at 2.0%) for negative staining of viruses and macromolecular } complexes, I have observed differences in stain distribution, } resolution and - interesting enough - also the optimum concentration } of the specimen. UA typically requires a less concentrated sample for } optimal results. } } Having said that, both stains can produce very nice results, but a } switch from one to the other might require adjustment of some } parameters in the protocol. } } Best regards, } } Guenter } } P.S: Do these NPs actually need to be stained? Depending on the } material/size/question at hand you might try to dry them down on a C } film, with or without washing. } } -- } Dr. Guenter Resch } Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net } Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 } Registered at Commercial Court Vienna, FN 397677w - VAT Reg. } ATU67962234 } ==============================Original } Headers============================== 8, 25 -- From } lists-at-nexperion.net Sat Jun 21 10:32:46 2014 8, 25 -- Received: from } 503.hosttech.eu (503.hosttech.eu [62.112.145.5]) 8, 25 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s5LFWjks006828 8, 25 -- for {Microscopy-at-microscopy.com} ; Sat, } 21 Jun 2014 10:32:45 -0500 8, 25 -- Received: from localhost (unknown } [127.0.0.1]) 8, 25 -- by 503.hosttech.eu (Postfix) with ESMTP } id 096746C0C2F; 8, 25 -- Sat, 21 Jun 2014 15:32:44 +0000 } (UTC) 8, 25 -- X-Virus-Scanned: amavisd-new at example.com 8, 25 -- } Received: from 503.hosttech.eu ([127.0.0.1]) 8, 25 -- by } localhost (503.hosttech.eu [127.0.0.1]) (amavisd-new, port 10024) 8, } 25 -- with ESMTP id MzTGgg2o6Lsq; Sat, 21 Jun 2014 17:32:37 } +0200 (CEST) 8, 25 -- Received: from aventine } (chello080108026214.8.11.vie.surfer.at [80.108.26.214]) 8, 25 -- } by 503.hosttech.eu (Postfix) with ESMTPSA id 05E4D6C0C2E; 8, } 25 -- Sat, 21 Jun 2014 17:32:36 +0200 (CEST) 8, 25 -- Date: } Sat, 21 Jun 2014 17:32:36 +0200 8, 25 -- From: Guenter Resch } {lists-at-nexperion.net} 8, 25 -- To: DusevichV-at-umkc.edu, } Microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] PTA vs } UA for negative staining 8, 25 -- Message-ID: } {20140621173236.75859bb9-at-aventine} 8, 25 -- In-Reply-To: } {201406201944.s5KJikb4024723-at-ns.microscopy.com} 8, 25 -- References: } {201406201944.s5KJikb4024723-at-ns.microscopy.com} 8, 25 -- X-Mailer: } Claws Mail 3.9.3 (GTK+ 2.24.23; x86_64-pc-linux-gnu) 8, 25 -- } MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; charset=US-ASCII } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 41 -- From benada-at-biomed.cas.cz Mon Jun 23 01:45:49 2014 4, 41 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 4, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5N6jmTx006826 4, 41 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jun 2014 01:45:49 -0500 4, 41 -- X-ASG-Debug-ID: 1403505946-05011e688108ef0001-4CH8be 4, 41 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id FRBzGrES6DP6UptD (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Mon, 23 Jun 2014 08:45:46 +0200 (CEST) 4, 41 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 4, 41 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 4, 41 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) 4, 41 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 4, 41 -- (No client certificate requested) 4, 41 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 98EEA2C846D 4, 41 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jun 2014 08:45:46 +0200 (CEST) 4, 41 -- Date: Mon, 23 Jun 2014 08:45:44 +0200 4, 41 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 4, 41 -- To: {microscopy-at-microscopy.com} 4, 41 -- Subject: Re: [Microscopy] Re: PTA vs UA for negative staining 4, 41 -- Message-ID: {20140623084544.70eb7a87-at-u117fs} 4, 41 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: PTA vs UA for negative staining 4, 41 -- In-Reply-To: {201406211538.s5LFceIt010793-at-ns.microscopy.com} 4, 41 -- References: {201406211538.s5LFceIt010793-at-ns.microscopy.com} 4, 41 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 4, 41 -- =?UTF-8?B?xIxS?= 4, 41 -- X-Mailer: Claws Mail 3.9.3 (GTK+ 2.24.10; i486-pc-linux-gnu) 4, 41 -- MIME-Version: 1.0 4, 41 -- Content-Type: text/plain; charset=US-ASCII 4, 41 -- Content-Transfer-Encoding: 7bit 4, 41 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 4, 41 -- X-Barracuda-Start-Time: 1403505946 4, 41 -- X-Barracuda-Encrypted: AES256-SHA 4, 41 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 4, 41 -- Received-SPF: none (biomed.cas.cz: benada-at-biomed.cas.cz does not designate permitted sender hosts) 4, 41 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 4, 41 -- X-Barracuda-BRTS-Status: 1 4, 41 -- X-Barracuda-Spam-Score: 0.50 4, 41 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M 4, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.6875 4, 41 -- Rule breakdown below 4, 41 -- pts rule name description 4, 41 -- ---- ---------------------- -------------------------------------------------- 4, 41 -- 0.50 BSF_RULE7568M Custom Rule 7568M ==============================End of - Headers==============================
Although most members of this listserver are hard-core microscopy professionals, some of your junior colleagues could benefit from attending Fourth Short Course on FIB technology and operation that will be taking place at University of Maryland at College Park, MD on 06/30 - 07/03.
Program covers basic principles of FIB, physical sputtering, Gas Assisted Etching/Deposition processes, and brief overview of practical applications. The course is open to all participants - please pass the information around and feel free to contact if you have any questions or would like to attend.
-- Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
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Others have already touched on the fact that FFT transforms of an HR image will have different phase information that the direct diffraction. Â I want to also mention that if you are computing your powder pattern using software designed for X-ray diffraction, it will usually exclude reflections which have a structure factor of zero. Â Many times, these reflections are visible in HR FFT transforms (and even in SAED diffractions). Â Therefore, you can expect to find more reflections in your HR FFT than you may guess. Â Also, expect the intensities to be very different from a calculated value.
In practical terms this can be very useful. Â Often the structure factor for large d-spacings is zero (e.g. 100 reflections are often not visible in X-ray diffraction) but these can show up in HR images. Â Since the d-spacings are large, they are usually much better discriminators of the crystal than higher order reflections.
Zack Gainsforth Space Sciences Laboratory, UC Berkeley 7 Gauss Way Berkeley, CA Â 94720 cell: 626-437-9186 zackg-at-ssl.berkeley.edu
This conversation brought to mind a recent experience we had here with silver-based nanoparticles. We don't have a specialised instrument for glow discharge, but we use a brief low KV, low amp pulse from our sputter coater (which we normally use for SEM sample coating). For most negative staining and other dried down samples, this works well.
However, for small nanoparticles (~10-20 nm diameter range) there is a problem. This procedure produces spots which are in that range and cannot be easily distinguished from nanoparticles. We know this because we always do controls. In our case we found it was not problem to examine these nanoparticles on grids which we had not treated with glow discharge. For the silver particles there was no need for negative stain of course.
I guess what I am saying is with nano-particles, make sure you do controls because there are numerous ways of particle-like artefacts of the sample prep process.
Good luck with it.
Duane
-----Original Message----- X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu] Sent: Tuesday, 24 June 2014 3:44 a.m. To: Harland, Duane
Debby, Thank you very much for reminding me about glow discharge (somehow it skipped away from me... )
Thanks to everybody for all replies. Particles are organic (pharma).
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: Sherman, Debra [mailto:dsherman-at-purdue.edu] Sent: Friday, June 20, 2014 3:46 PM To: Dusevich, Vladimir
==============================Original Headers============================== 16, 31 -- From DusevichV-at-umkc.edu Mon Jun 23 10:29:42 2014 16, 31 -- Received: from um-tip2-umkc-out.um.umsystem.edu (um-tip2-umkc-out.um.umsystem.edu [198.209.49.152]) 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5NFTg1e001639 16, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2014 10:29:42 -0500 16, 31 -- X-IronPort-Anti-Spam-Filtered: true 16, 31 -- X-IronPort-Anti-Spam-Result: AlkFAFxHqFPPoJ7G/2dsb2JhbABWA4MNUlrCXoEhAYEOFnWEAwEBAQQ6FDcEAgEIEQMBAQELAgIQCQcxARQJCAIECgkIFQSIIQ3AVIVzF45LBhsSBQYLgxyBFgSuL4NCbIFE 16, 31 -- X-IPAS-Result: AlkFAFxHqFPPoJ7G/2dsb2JhbABWA4MNUlrCXoEhAYEOFnWEAwEBAQQ6FDcEAgEIEQMBAQELAgIQCQcxARQJCAIECgkIFQSIIQ3AVIVzF45LBhsSBQYLgxyBFgSuL4NCbIFE 16, 31 -- Received: from um-tcas2.um.umsystem.edu ([207.160.158.198]) 16, 31 -- by um-tip2-exch-relay.um.umsystem.edu with ESMTP; 23 Jun 2014 10:29:40 -0500 16, 31 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.129]) by 16, 31 -- UM-TCAS2.um.umsystem.edu ([207.160.158.198]) with mapi id 14.03.0181.006; 16, 31 -- Mon, 23 Jun 2014 10:29:40 -0500 16, 31 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 16, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 16, 31 -- Subject: RE: [Microscopy] PTA vs UA for negative staining 16, 31 -- Thread-Topic: [Microscopy] PTA vs UA for negative staining 16, 31 -- Thread-Index: AQHPjLy7q78cWxbV/0GNSIZB9mkco5t6d86AgARX+dA= 16, 31 -- Date: Mon, 23 Jun 2014 15:29:39 +0000 16, 31 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DBD7EB2-at-UM-MBX-T01.um.umsystem.edu} 16, 31 -- References: {201406201920.s5KJKgrJ009381-at-ns.microscopy.com} 16, 31 -- {CFCA1539.1F210%dsherman-at-purdue.edu} 16, 31 -- In-Reply-To: {CFCA1539.1F210%dsherman-at-purdue.edu} 16, 31 -- Accept-Language: en-US 16, 31 -- Content-Language: en-US 16, 31 -- X-MS-Has-Attach: 16, 31 -- X-MS-TNEF-Correlator: 16, 31 -- x-originating-ip: [134.193.156.218] 16, 31 -- Content-Type: text/plain; charset="us-ascii" 16, 31 -- MIME-Version: 1.0 16, 31 -- Content-Transfer-Encoding: 8bit 16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5NFTg1e001639 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 37 -- From Duane.Harland-at-agresearch.co.nz Mon Jun 23 16:03:36 2014 30, 37 -- Received: from mx1.securemx.biz (mx1.securemx.biz [203.84.134.2]) 30, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5NL3YXI030834 30, 37 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jun 2014 16:03:35 -0500 30, 37 -- DKIM-Signature: v=1; a=rsa-sha256; d=nz.smxemail.com; s=alpha; c=relaxed/relaxed; 30, 37 -- q=dns/txt; i=-at-nz.smxemail.com; t=1403557414; 30, 37 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc; 30, 37 -- bh=csBzTmR2fCWkXRGMMxWoH3LZumDDgRZCEubJjMjQJHw=; 30, 37 -- b=eOCx52punRGw9kZxY6rTLWbVWtrEvD8mDeNj2GVqpXTGQ0JAGrT0WpQ6hrPQo3Ij 30, 37 -- Awe0+YPDjy3qugnwSdjquAB5Xfp4Eu0XDHeFFLqYaNpc3OKA+7vAs+eHqEhZZKFu 30, 37 -- ApipHXqiwYkopMnMMezgfUJpHs4oMOhkebqujk/8sTk=; 30, 37 -- Received: from invexcpv02.agresearch.co.nz ([202.20.102.50]) 30, 37 -- by omr.nz.smxemail.com with ESMTP (using TLSv1 30, 37 -- with cipher AES128-SHA (128/128 bits)) 30, 37 -- id 53A89622-3C8E0AB3-at-mta1103.omr; 30, 37 -- Mon, 23 Jun 2014 21:03:30 +0000 30, 37 -- Received: from INVEXCPV01.agresearch.co.nz ([169.254.1.18]) by 30, 37 -- invexcpv03.agresearch.co.nz ([147.158.130.6]) with mapi id 14.03.0174.001; 30, 37 -- Tue, 24 Jun 2014 09:03:17 +1200 30, 37 -- From: "Harland, Duane" {Duane.Harland-at-agresearch.co.nz} 30, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 30, 37 -- Subject: RE: [Microscopy] RE: PTA vs UA for negative staining 30, 37 -- Thread-Topic: [Microscopy] RE: PTA vs UA for negative staining 30, 37 -- Thread-Index: AQHPjvnoe/adZTqZDky17nI/IgQVPJt/LLwg 30, 37 -- Date: Mon, 23 Jun 2014 21:03:16 +0000 30, 37 -- Message-ID: {C1CB2BCA7689064DA55A366147C8BEF157A9582B-at-invexcpv01.agresearch.co.nz} 30, 37 -- References: {201406231543.s5NFhY3m019092-at-ns.microscopy.com} 30, 37 -- In-Reply-To: {201406231543.s5NFhY3m019092-at-ns.microscopy.com} 30, 37 -- Accept-Language: en-GB, en-NZ, en-US 30, 37 -- Content-Language: en-US 30, 37 -- X-MS-Has-Attach: 30, 37 -- X-MS-TNEF-Correlator: 30, 37 -- x-originating-ip: [147.158.192.179] 30, 37 -- Content-Type: text/plain; charset="us-ascii" 30, 37 -- MIME-Version: 1.0 30, 37 -- Content-Transfer-Encoding: 8bit 30, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5NL3YXI030834 ==============================End of - Headers==============================
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Email: ktkoch-at-ameslab.gov Name: Kurt Koch
Organization: Ames lab
Title-Subject: [Filtered] model 656 dimpler
Message: I was wondering if anyone had an old Gatan model 656 dimpler that was no good any more. I'd really like to get the turntable motor out of one.
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Email: vicenzi_-at-msn.com Name: Ed. Vicenzi
Organization: IUMAS
Title-Subject: [Filtered] IUMAS-6
Message: The International Union of Microbeam Analysis Societies 6th Meeting (IUMAS-6)*
Come a few days early to M&M 2014 in Hartford and experience an excellent international microanalysis meeting!
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jun 23 21:31:27 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5O2VQRM004724 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jun 2014 21:31:26 -0500 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 8487C71EAA4 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jun 2014 21:31:26 -0500 (CDT) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id b2d64mjLhOqC for {microscopy-at-microscopy.com} ; 19, 28 -- Mon, 23 Jun 2014 21:31:24 -0500 (CDT) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 86C5771EA97 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jun 2014 21:31:24 -0500 (CDT) 19, 28 -- Message-ID: {53A8E2FC.6010404-at-microscopy.com} 19, 28 -- Date: Mon, 23 Jun 2014 21:31:24 -0500 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:IUMAS-6 19, 28 -- References: {201406240225.s5O2P6e1000353-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201406240225.s5O2P6e1000353-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201406240225.s5O2P6e1000353-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From medications-cheapest8-at-intergorodok.com.ua Tue Jun 24 03:17:03 2014 Return-Path: {medications-cheapest8-at-intergorodok.com.ua} Received: from zarya.intergorodok.com.ua ([176.121.192.68]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5O8Gx9K012583 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 24 Jun 2014 03:17:01 -0500 Message-Id: {201406240817.s5O8Gx9K012583-at-ns.microscopy.com}
X-from: alpinto-at-medicina.ulisboa.pt ()
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Title-Subject: [Filtered] Reichert Jung ultracut E spares
Message: Hi to all,
Recently my ultramicrotome Reichert Jung ultracut E had a problem in the motor that moves the sample forward during sectioning, as expected, regarding the age, this motor is hard to find and probably impossible to replace by a new one (the Berger/Lahr company no longer exists), so I am looking for a used motor (hope you can see it in the link below) the reference is: Berger/Lahr, W-germany, RDM253/50, IW= 240MA,RW= 37.5ş. Does anyone has or know someone, or some company that has/sells (second handed) this motor? like maybe an old ultramicrotome (same brand and model)with spare parts to share.
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Jun 24 06:48:59 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5OBmxL9015306 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jun 2014 06:48:59 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id 27D0671EF01 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jun 2014 06:48:59 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id 9i6-hA2rTTKO for {microscopy-at-microscopy.com} ; 17, 28 -- Tue, 24 Jun 2014 06:48:55 -0500 (CDT) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 1D1EF71EEEF 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jun 2014 06:48:55 -0500 (CDT) 17, 28 -- Message-ID: {53A965A6.2020606-at-microscopy.com} 17, 28 -- Date: Tue, 24 Jun 2014 06:48:54 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW: Reichert Jung ultracut E spares 17, 28 -- References: {201406240914.s5O9EKug013333-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201406240914.s5O9EKug013333-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201406240914.s5O9EKug013333-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From drugs_popular4-at-pride-net.ru Wed Jun 25 01:53:24 2014 Return-Path: {drugs_popular4-at-pride-net.ru} Received: from 91-48-251-80.pride-net.ru (91-48-251-80.pride-net.ru [80.251.48.91]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5P6rJoP017807 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 25 Jun 2014 01:53:20 -0500 Message-Id: {201406250653.s5P6rJoP017807-at-ns.microscopy.com} X-AntiVirus: Checked by Dr.Web [version: 7.0.4.09220, engine: 7.0.100.09170, virus records: 5340345, updated: 25.06.2014]
Structure Probe, Inc. is seeking an electron microscopist to work in its West Chester, PA analytical laboratory. We are a materials science oriented facility providing analytical services in the electron microscopy field to our clients on a contract basis. This individual should possess TEM skills including microtomy capabilities. BS or 5-years experience preferred. Duties include sample preparation, obtaining analytical data, working with clients and report preparation. Further information may be found on our website at: http://www.2spi.com/employment-opp.html
Eugene Rodek Vice President SPI Supplies erodek-at-2spi.com www.2spi.com
==============================Original Headers============================== 4, 26 -- From erodek-at-2spi.com Wed Jun 25 14:01:01 2014 4, 26 -- Received: from SPI-EX-01.spi.local (mail.2spi.com [209.120.197.107]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5PJ118P027615 4, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Jun 2014 14:01:01 -0500 4, 26 -- Received: from SPI-EX-01.spi.local (172.25.75.31) by SPI-EX-01.spi.local 4, 26 -- (172.25.75.31) with Microsoft SMTP Server (TLS) id 15.0.516.32; Wed, 25 Jun 4, 26 -- 2014 15:00:33 -0400 4, 26 -- Received: from SPI-EX-01.spi.local ([fe80::f8c4:9b23:d8de:fa70]) by 4, 26 -- SPI-EX-01.spi.local ([fe80::f8c4:9b23:d8de:fa70%12]) with mapi id 4, 26 -- 15.00.0516.029; Wed, 25 Jun 2014 15:00:33 -0400 4, 26 -- From: Gene Rodek {erodek-at-2spi.com} 4, 26 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 4, 26 -- Subject: Job Opportunity - Electron Microscopist 4, 26 -- Thread-Topic: Job Opportunity - Electron Microscopist 4, 26 -- Thread-Index: Ac+Qp24oxRvPmSJtRRaUdcjceFEbCA== 4, 26 -- Date: Wed, 25 Jun 2014 19:00:33 +0000 4, 26 -- Message-ID: {1824be4f773e48b897964e9c59324418-at-SPI-EX-01.spi.local} 4, 26 -- Accept-Language: en-US 4, 26 -- Content-Language: en-US 4, 26 -- X-MS-Has-Attach: 4, 26 -- X-MS-TNEF-Correlator: 4, 26 -- x-originating-ip: [172.25.75.108] 4, 26 -- Content-Type: text/plain; charset="us-ascii" 4, 26 -- MIME-Version: 1.0 4, 26 -- Content-Transfer-Encoding: 8bit 4, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5PJ118P027615 ==============================End of - Headers==============================
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Email: dan.fairweather-at-delphi.com Name: Dan Fairweather
Organization: Delphi Automotive, Powertrain Div
Title-Subject: [Filtered] Hitachi S3200-N variable pressure control
Message: This morning I experienced a situation on our SEM that hasn't occurred before. While operating in variable pressure mode, the system will not control below 100 Pa. I normally operate -at- 30 Pa for non-conductive samples. It is an older system and the air supply lines are starting to fail. But I don't hear any air leaks when I remove the front of the cabinet. We did verify the fluid levels in our roughing pumps and topped them both.
We do have a call in to Hitachi service, but they can't get to us until July. Does any one have experience with this problem? Are there some suggestions you can make to diagnose the problem?
Thank you in advance, Dan Fairweather Senior Mateials Development Engineer Delphi Automotive
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] Chiller problem
Message: Hi!
The chiller of FEI HRTEM F30 is consistently at 10 deg C. Even after increasing the tempt. it is not increasing. I would like to know the possible reasons: is it due to the faulty temp. controller or sensor?
Thanks and Regards Rashmi
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We had a S-2460N for many years. In fact, we just shipped it off working a week ago. I think it may have had a similar problem.
About every six months to a year, the system would stop controlling and we would find that the needle valve that determines the chamber pressure had bound up. Under the original setup, the valve would never really settle down. It remained in an oscillating mode turning a half turn this way then a half turn back. The valve was always in motion. Eventually the threads galled and it became hard to turn. You could still turn it by hand - with difficulty, but the motor was not up to the task. Hitachi eventually got it figured out and changed some components in the control board so that it did settle in and the valve turned much less and lasted much longer.
Our valve was located in the right rear of the column cabinet near the top. We could remove the back panel and watch the valve in action. As I recall, when we had problems, we would unplug the motor cable and set the valve by hand for the desired pressure. I think there was another line used to vent the chamber so we could just about "set it and forget it". Of course, Hitachi then came in, replaced the valve, and we were back on our regular routine.
Contact me if you want more details.
Warren
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X-from: dan.fairweather-at-delphi.com ()
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Email: dan.fairweather-at-delphi.com Name: Dan Fairweather
Organization: Delphi Automotive, Powertrain Div
Title-Subject: [Filtered] Hitachi S3200-N variable pressure control
Message: This morning I experienced a situation on our SEM that hasn't occurred before. While operating in variable pressure mode, the system will not control below 100 Pa. I normally operate -at- 30 Pa for non-conductive samples. It is an older system and the air supply lines are starting to fail. But I don't hear any air leaks when I remove the front of the cabinet. We did verify the fluid levels in our roughing pumps and topped them both.
We do have a call in to Hitachi service, but they can't get to us until July. Does any one have experience with this problem? Are there some suggestions you can make to diagnose the problem?
Thank you in advance, Dan Fairweather Senior Mateials Development Engineer Delphi Automotive
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==============================Original Headers============================== 25, 33 -- From wesaia-at-iastate.edu Wed Jun 25 21:19:53 2014 25, 33 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2lp0241.outbound.protection.outlook.com [207.46.163.241]) 25, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5Q2JqW6012358 25, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2014 21:19:52 -0500 25, 33 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 25, 33 -- BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) with Microsoft SMTP 25, 33 -- Server (TLS) id 15.0.969.15; Thu, 26 Jun 2014 02:19:51 +0000 25, 33 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.109]) by 25, 33 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.49]) with mapi id 25, 33 -- 15.00.0969.007; Thu, 26 Jun 2014 02:19:51 +0000 25, 33 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 25, 33 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 25, 33 -- CC: "dan.fairweather-at-delphi.com" {dan.fairweather-at-delphi.com} 25, 33 -- Subject: RE: [Microscopy] viaWWW:Hitachi S3200-N variable pressure control 25, 33 -- Thread-Topic: [Microscopy] viaWWW:Hitachi S3200-N variable pressure control 25, 33 -- Thread-Index: AQHPkOMoD11lyNpuJk2QHcAwp7QHq5uCpUtg 25, 33 -- Date: Thu, 26 Jun 2014 02:19:50 +0000 25, 33 -- Message-ID: {dd4973b51cd149efa0e7eadc91b049cf-at-BLUPR04MB546.namprd04.prod.outlook.com} 25, 33 -- References: {201406260205.s5Q25fTN019304-at-ns.microscopy.com} 25, 33 -- In-Reply-To: {201406260205.s5Q25fTN019304-at-ns.microscopy.com} 25, 33 -- Accept-Language: en-US 25, 33 -- Content-Language: en-US 25, 33 -- X-MS-Has-Attach: 25, 33 -- X-MS-TNEF-Correlator: 25, 33 -- x-originating-ip: [69.66.102.132] 25, 33 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID: 25, 33 -- x-forefront-prvs: 02543CD7CD 25, 33 -- x-forefront-antispam-report: SFV:NSPM;SFS:(6009001)(189002)(252514010)(199002)(13464003)(504964003)(377454003)(15975445006)(86362001)(20776003)(83322001)(31966008)(50986999)(99396002)(15202345003)(74662001)(85306003)(54356999)(85852003)(92566001)(77096002)(101416001)(107046002)(64544003)(19580395003)(2656002)(81342001)(64706001)(19580405001)(33646001)(80022001)(81542001)(87936001)(83072002)(75432001)(105586002)(106356001)(74316001)(88552001)(21056001)(76176999)(76482001)(46102001)(74502001)(79102001)(4396001)(95666004)(77982001)(106116001)(99286002)(76576001)(24736002);DIR:OUT;SFP:;SCL:1;SRVR:BLUPR04MB546;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 25, 33 -- Content-Type: text/plain; charset="us-ascii" 25, 33 -- MIME-Version: 1.0 25, 33 -- X-OriginatorOrg: iastate.edu 25, 33 -- Content-Transfer-Encoding: 8bit 25, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5Q2JqW6012358 ==============================End of - Headers==============================
From cheapest-meds4-at-dalacom.kz Wed Jun 25 23:37:43 2014 Return-Path: {cheapest-meds4-at-dalacom.kz} Received: from akx-p132.dalacom.kz (akx-p132.dalacom.kz [217.76.76.132]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5Q4bejP011764 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 25 Jun 2014 23:37:41 -0500 Message-Id: {201406260437.s5Q4bejP011764-at-ns.microscopy.com}
Hi all,
I know this has been discussed before, just wondered if there were any new thoughts. We're moving twice, first into a refurbished basement, second time into a new building. The basement has a sprinkler system in case of fire, unlike our current building which just has alarms (it's a single storey brick building). I imagine the new building will have some sort of sprinkler system too.
What precautions, if any, do people take to protect against the unlikely showering of your confocal or EM? Are there any relatively simple alternatives? (One alternative is to have fire-doors and walls for each room but that is prohibitive.)
thanks much, Rosemary
Dr Rosemary White CSIRO Plant Industry (for 4 more days only) GPO Box 1600 Canberra, ACT 2601
T 02 6246 5475 F 02 6246 5334 E rosemary.white-at-csiro.au
} The chiller of FEI HRTEM F30 is consistently at 10 deg C. Even after increasing the tempt. it is not } increasing. I would like to know the possible reasons: is it due to the faulty temp. controller or } sensor?
did you measure the 10 degrees yourself or is this what is displayed on the cooling unit? This might help to identify if the sensor is broken or not.
Depending on manufacturer, another culprit might be the valve that controls the flow of water of the secondary cooling circuit through either the heat exchanger or the bypass - if that is stuck, you are constantly cooling.
Did you already try to contact the manufacturer of the cooling unit?
Best,
Guenter
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
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If your chiller is from Haskris, you can call them for "over the phone diagnostics". I have done it already twice; bad parts were correctly identified, ordered, and replaced by myself (my chillers are old...)
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, June 25, 2014 9:07 PM To: Dusevich, Vladimir
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] Chiller problem
Message: Hi!
The chiller of FEI HRTEM F30 is consistently at 10 deg C. Even after increasing the tempt. it is not increasing. I would like to know the possible reasons: is it due to the faulty temp. controller or sensor?
Thanks and Regards Rashmi
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==============================Original Headers============================== 20, 30 -- From DusevichV-at-umkc.edu Thu Jun 26 09:20:36 2014 20, 30 -- Received: from um-tip1-umkc-out.um.umsystem.edu (um-tip1-umkc-out.um.umsystem.edu [198.209.49.138]) 20, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5QEKaAF024566 20, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2014 09:20:36 -0500 20, 30 -- X-IronPort-Anti-Spam-Filtered: true 20, 30 -- X-IronPort-Anti-Spam-Result: AqoHAOErrFPPoJ7S/2dsb2JhbABXA4MNUlqtB44FiHUBgQsWdYQDAQEBBDoUNwQCAQgRAwEBAQsCCgEHCQcxARMBCQgCBAoJCBUEiA0DEQ29KIV5F4xbCxKBJhEBHyESBQYLAQGDGoEWBYRjBXqHBwKLLEqDPYwchg2DQmyBBAcXIg 20, 30 -- X-IPAS-Result: AqoHAOErrFPPoJ7S/2dsb2JhbABXA4MNUlqtB44FiHUBgQsWdYQDAQEBBDoUNwQCAQgRAwEBAQsCCgEHCQcxARMBCQgCBAoJCBUEiA0DEQ29KIV5F4xbCxKBJhEBHyESBQYLAQGDGoEWBYRjBXqHBwKLLEqDPYwchg2DQmyBBAcXIg 20, 30 -- Received: from um-ncas5.um.umsystem.edu ([207.160.158.210]) 20, 30 -- by um-tip1-exch-relay.um.umsystem.edu with ESMTP; 26 Jun 2014 09:20:15 -0500 20, 30 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.129]) by 20, 30 -- UM-NCAS5.um.umsystem.edu ([207.160.158.210]) with mapi id 14.03.0181.006; 20, 30 -- Thu, 26 Jun 2014 09:20:15 -0500 20, 30 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 20, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 20, 30 -- Subject: RE: [Microscopy] Re: viaWWW:Chiller problem 20, 30 -- Thread-Topic: [Microscopy] Re: viaWWW:Chiller problem 20, 30 -- Thread-Index: AQHPkONT9KHF8Wxz/E+cZKoYObErfJuDcYIg 20, 30 -- Date: Thu, 26 Jun 2014 14:20:14 +0000 20, 30 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB5828065DBD8171-at-UM-MBX-T01.um.umsystem.edu} 20, 30 -- References: {201406260207.s5Q2729v020536-at-ns.microscopy.com} 20, 30 -- In-Reply-To: {201406260207.s5Q2729v020536-at-ns.microscopy.com} 20, 30 -- Accept-Language: en-US 20, 30 -- Content-Language: en-US 20, 30 -- X-MS-Has-Attach: 20, 30 -- X-MS-TNEF-Correlator: 20, 30 -- x-originating-ip: [134.193.156.218] 20, 30 -- Content-Type: text/plain; charset="us-ascii" 20, 30 -- MIME-Version: 1.0 20, 30 -- Content-Transfer-Encoding: 8bit 20, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5QEKaAF024566 ==============================End of - Headers==============================
Dear EM slot grid users I had some experience making this support - membrane - film for slot grid with formvar or butvar (pioloform). But as you may know this more an art than a highly reproducible protocol... Very briefly: For the one who experienced to make such membrane there are two main critical steps: First critical step is cleaning the glass slide with kimwipe then with lens tissue or with 70% ethanol then kimwipe then with lens tissue or dip in alconox saturated solution then without rincing kimwipe then with lens tissue
Casting the film on the glass slide put the cleaned glass slide in a borel glass container half filled with your solution of formvar or butvar. Instead of a borel I use a homemade film casting device resembling the one of EMS with two part glass allowing a controlled flux from the upper part containing the glass slide to the lower container with a special screw: "A built-in fine-pressure-release valve helps control the speed of drainage." EMS 71305-01 I used both dissolved around 0.30-0.60% butvar in chloroform (high quality EMSURE from Merck stabilized with 0.6% ethanol)
Detaching the film on a large and deep glass container Some people use a blade to score the film few millimeter parallel to the long side of the glass slide and at its bottom, some rub the 3 side of the glass slide against a kimwipe.
Some people insert the glass slide in the water with a 90° angle allowing the detachment of the membrane from the two sides of th glass slide. Some insert it with a 45° angle detaching only the upper membrane. In my hands both work well.
I have many questions regarding this procedure. How do you keep your solution of butvar ? at 4C° ? at RT ? and for how long do you keep it ? As you know the problem is the humidity in the room when your cast your membrane: I have read that some people do not cast when the air is humid !!! do you have a way to control this ? I tried to put some nusiev bead in the butvar solution the membrane detached well but I haven't check the quality of the membrane yet. Do you have experience with this ?
I should definitely have some feed back on this Oh so delicate issue
Thank you very much Jean-Michel Hermel
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Dear Jean-Michel, congratulations for summing up all these important steps in mounting films to grids. Thank you! A similar MSA thread has been presented / started July 18, 2012, entitled: ("[Microscopy] formvar grids" by Nicki Watson]. Unfortunately I have no experience with butvar-, but with formvar-films.
In your summary, for:
{Some people insert the glass slide in the water with a 90° angle allowing the detachment of the membrane from the two sides of the glass slide. Some insert it with a 45° angle detaching only the upper membrane. In my hands both work well.}
I would like to add that some people try {breathing} to the glass slide with the film before inserting the slide into the water because this could help in easier detachment of the film. In my experience inserting angles between 45 and 85 degr. will work. I sometimes was able, to detach the formvar film from BOTH sides of the glass slide inserting them at really 90 degr.
Regarding your questions: Humidity: if the air in the working room is too humid DURING FILM application, you will get holes in your film (but this depending also on the apparatus used for application of formvar.... unfortunately I could not find any German translation for {BOREL} glass container, so I cannot comprehend what / how you are doing films with such one.
I am using a self-made film casting device (principally very similar to the EMS Film Casting device you described below (cf: https://www.emsdiasum.com/microscopy/products/grids/accessories.aspx and scroll down to the middle of the display in the window).
For such a device it needs therefore primarily a formvar or butvar (pioloform) organic diluent which i) is 100% anhydrous (i.e. either freshly made) or (since as with the EMS-model: {The film casting solution can be used repeatedly} ) ii) the possibility of a closed / totally closable/sealable system during storage of the Formvar/Butvar-solution for a longer time.
During the making of film (application to the cleaned glass slide) you normally will have arid conditions if you don't insert a humid or moist glass slide, which - if applied consecutively several times - certainly (w)could add moisture to the (anhydrous) organic solution which in turn produced "holey" films (this was -BTW - an instruction I was given by a famous ZEISS application rep. in 1981 when I started my EM-career, namely: to direct a short puff of "moisty" breath onto the glass slide receptaculum or the glass slide itself at the time the Formvar/Butvar solution completely sank down within the casting cylinder below the lower glass slide edge). Control for moisture (i.e. {dry} environment during the film application process in the cylinder - i) if this is an open system "upwards" from where you place the object slide for film application): mounting a phiole (can be made from glass or even plastic, the latter can be purchased as "test tubes" with both sided detachable "olives" ) containing granulate of moisture absorbing material (some kind of silica gel containing a color-indicator) ii) the same applies for the Air-in and Air-out atomizer ball (in between ball and nozzle linking to the {drainage neck} of the apparatus) Such construction will help in keeping the organic solution as "dry/anhydrous" as possible.
Storage of the device/solution when not in operation: i) shield from light all time when not using the apparatus [fitting cardboard box, eventually optimized by own hands on...(:-))] ii) storage at ambient (Room) Temperature (not in refrigerator since you will get moisture in it when "warming up to working temperature) iii) shelf life of solution: I have made the experience that one can use a more concentrated stock solution in really (99.9%) anhydrous organic solution (e.g. Chloroform UVASOL [Methane trichloride, Trichloromethane] for more than a year, diluted solutions from that - if stored as working solution properly after dilution (light shielding and system firmly closed), for at least 1 year too. Other diluents, like ethylene dichloride [1,2-Dichloroethane; Ethylene chloride; Glycol dichloride] or Dioxane [Diethylene dioxide; Diethylene ether; Dioxan; p-Dioxane] I don't have an experience.
Eventually cf: Work-Study Student Handbook, Chapter 7. Grid Preparation Methods: -at- http://metz.rose2.brandeis.edu/emdoc/books/workstudystudents-handbook/grids.html esp. cf: http://metz.rose2.brandeis.edu/emdoc/books/workstudystudents-handbook/grids-coated.html as well as PREV & NEXT pages! esp. cf: Making Formvar Grids in High Humidity: http://metz.rose2.brandeis.edu/emdoc/books/workstudystudents-handbook/grids-humidity.html
One question left: what did you mean when writing: {I tried to put some { {'nusiev'} } bead in the butvar solution} ? is this a kind of molecular sieve??
By the way I have found a "classic, ancient" literature reference for Collodion/Amylacetat and Formvar/chloroform filmed Grids: SPECIMEN PREPARATION FOR ELECTRON MICROSCOPY, by J.A. GARD, 1955, 5 pages (http://www.minersoc.org/pages/Archive-CM/Volume_3/3-15-14.pdf)
Best wishes and regards, Wolfgang
Wolfgang MUSS MSA-member SALZBURG, Austria
Von: jmhermel-at-inaf.cnrs-gif.fr [mailto:jmhermel-at-inaf.cnrs-gif.fr] Gesendet: Donnerstag, 26. Juni 2014 17:39 An: MuĂź Wolfgang Betreff: [Microscopy] support membrane film for slot grid
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Dear EM slot grid users, I had some experience making this support - membrane - film for slot grid with formvar or butvar (pioloform).
But as you may know this more an art than a highly reproducible protocol... Very briefly: For the one who experienced to make such membrane there are two main critical steps:
First critical step is cleaning the glass slide with kimwipe then with lens tissue or with 70% ethanol then kimwipe then with lens tissue or dip in alconox saturated solution then without rincing kimwipe then with lens tissue
Casting the film on the glass slide put the cleaned glass slide in a borel glass container half filled with your solution of formvar or butvar. Instead of a borel I use a homemade film casting device resembling the one of EMS with two part glass allowing a controlled flux from the upper part containing the glass slide to the lower container with a special screw: "A built-in fine-pressure-release valve helps control the speed of drainage." EMS 71305-01 I used both dissolved around 0.30-0.60% butvar in chloroform (high quality EMSURE from Merck stabilized with 0.6% ethanol)
Detaching the film on a large and deep glass container Some people use a blade to score the film few millimeter parallel to the long side of the glass slide and at its bottom, some rub the 3 side of the glass slide against a kimwipe.
Some people insert the glass slide in the water with a 90° angle allowing the detachment of the membrane from the two sides of the glass slide. Some insert it with a 45° angle detaching only the upper membrane. In my hands both work well.
I have many questions regarding this procedure. How do you keep your solution of butvar ? at 4C° ? at RT ? and for how long do you keep it ?
As you know the problem is the humidity in the room when your cast your membrane: I have read that some people do not cast when the air is humid !!! do you have a way to control this ?
I tried to put some nusiev bead in the butvar solution the membrane detached well but I haven't check the quality of the membrane yet. Do you have experience with this ?
I should definitely have some feed back on this Oh so delicate issue
Thank you very much Jean-Michel Hermel
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==============================Original Headers============================== 39, 38 -- From W.Muss-at-salk.at Thu Jun 26 13:19:31 2014 39, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 39, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5QIJUMR001118 39, 38 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2014 13:19:30 -0500 39, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 39, 38 -- by hermes.salk.at (Postfix) with ESMTP id BD3C3646B41 39, 38 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2014 20:19:28 +0200 (CEST) 39, 38 -- X-Scanned-By: SALK-Content-Filter 39, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 39, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 39, 38 -- with ESMTP id eWkNKGmYCeKo for {microscopy-at-microscopy.com} ; 39, 38 -- Thu, 26 Jun 2014 20:19:28 +0200 (CEST) 39, 38 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 39, 38 -- by hermes.salk.at (Postfix) with ESMTP id 7E612646B2D 39, 38 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2014 20:19:28 +0200 (CEST) 39, 38 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 39, 38 -- by N1EX198.lks.local with ESMTP; 26 Jun 2014 20:29:07 +0200 39, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 39, 38 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 39, 38 -- 14.02.0387.000; Thu, 26 Jun 2014 20:19:28 +0200 39, 38 -- From: =?utf-8?B?TXXDnyBXb2xmZ2FuZw==?= {W.Muss-at-salk.at} 39, 38 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 39, 38 -- Subject: [Microscopy] Re: support membrane film for slot grid 39, 38 -- Thread-Topic: [Microscopy] Re: support membrane film for slot grid 39, 38 -- Thread-Index: AQHPkVnVo1gE92VI1EicVHFj/ZGIlA== 39, 38 -- Date: Thu, 26 Jun 2014 18:19:27 +0000 39, 38 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D39E9B1-at-N2DT131.lksdom21.lks.local} 39, 38 -- References: {201406261539.s5QFdEHX016578-at-ns.microscopy.com} 39, 38 -- In-Reply-To: {201406261539.s5QFdEHX016578-at-ns.microscopy.com} 39, 38 -- Accept-Language: de-DE, en-US 39, 38 -- Content-Language: de-DE 39, 38 -- X-MS-Has-Attach: 39, 38 -- X-MS-TNEF-Correlator: 39, 38 -- x-originating-ip: [192.168.42.2] 39, 38 -- Content-Type: text/plain; charset="utf-8" 39, 38 -- MIME-Version: 1.0 39, 38 -- Content-Transfer-Encoding: 8bit 39, 38 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s5QIJUMR001118 ==============================End of - Headers==============================
From drugstore-popular5-at-kurkino.net.ru Thu Jun 26 20:00:00 2014 Return-Path: {drugstore-popular5-at-kurkino.net.ru} Received: from OrbitaTelecom-Net-91-206-15-Ip-60.kurkino.net.ru ([91.206.15.60]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5R0xvLA000494 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 26 Jun 2014 19:59:58 -0500 Message-Id: {201406270059.s5R0xvLA000494-at-ns.microscopy.com}
Hi Rosemary
Out of interest I have dealt with water cascading over microscopes, yes it has happened twice in my 49 electron microscopy years. As a service technician the main problem is not so much the water, but the debris that the water beings down onto the electrical circuits. Both of the instruments in question were running on vacuum only and managed to keep going overnight until security found the water running through the particular laboratories. One was a tap exploding, shooting a fountain of water into the EM room, the other the result of putting out a fire three levels above.
A third situation occurred when a water pipe came off one instrument in a basement EM unit, flooding the complete area to about 6 inches. Everyone of the instruments kept going, but as I arrived a technician with rubber boots and a long wooden pole was taking care of switching the most endangered instruments off. However this was in the 1980s when most EM Units were underground; the one I mentioned was also used as a Tornado shelter!
EM Units and water, great fun!
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Sent: 26 June 2014 11:09 To: protrain-at-emcourses.com
X-from: joseph.uknalis-at-ars.usda.gov ()
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: ERRC - USDA
Title-Subject: [Filtered] new sputter coater recommendation
Message: Hi folks- Looking for a new sputter coater-
considering:
EM Sciences EMS150R
Denton Standard Desk V HP
Leica EM ACE200
or any other you may prefer.
please contact me off list.
thanks
Joe
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Email: andrei.kolmakov-at-nist.gov Name: Andrei Kolmakov
Organization: NIST
Title-Subject: [Filtered] coater
Message: Dear Colleagues, We are considering purchasing table-top high vacuum coater as a general user friendly instrument which would allow : 1. sputter coating (exchangeable targets) 2. carbon coating 3. thermal and/or e-beam metal evaporation 4. glow discharge treatment and/or cleaning We are considering Leica Microsystems AC600 and Electron Microscopy Sciences EMS 150T ES systems. Your comments/experiences on their performance would be a great help. Thank you in advance Andrei
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Email: jjmccarthy-at-wisc.edu Name: Jon McCarhty
Organization: College i=of Engineering, UW Madison
Title-Subject: [Filtered] Research Facilities Director Posting
Message: HI all;
WITH MY IMPENDING RETIREMENT, MY POSITION IS NOW POSTED. Please read and submit if interested. Deadline is coming up on July one, but an email to Susan Hagness with expressed interesT is advised to get an extension!
Best to all, and see you at IUMAS/MAs!
Jon McCarthy, Past MAS President _____________________________________________ Vacancy Listing #79754 to: Susan Hagness Phone: 608-265-5739
1415 Engineering Dr 2620 Engineering Hall Email: hagness-at-engr.wisc.edu
Working Title: RESEARCH USER FACILITIES DIRECTOR Official Title: SENIOR SCIENTIST OR ASSOCIATE SCIENTIST
Degree and area of specialization: Ph.D. in Engineering or the Physical Sciences with extensive knowledge in the field of scientific instrumentation, microscopy, research programs and specialized research equipment.
Minimum number of years and type of relevant work experience: Five years of experience in scientific and research environments, including the private sector, national laboratories, and/or higher education. Experience also includes successful program development, demonstrated knowledge and use of advanced characterization tools or micro-fabrication technologies and managing budgets and personnel. Candidates should have a demonstrated commitment to excellence in scientific leadership.
Position Overview The Research User Facilities Director will report to the Associate Dean for Research and Graduate Affairs and will be responsible for all aspects of managing the College of Engineering research user facilities which include, but are not limited to, the Materials Science Center, Soft Materials Laboratory and the Wisconsin Center for Applied Microelectronics.
Principal Duties: • Construct a business plan for each research user facility that includes development of annual budgets, establishment of user rates, anticipated customer base and customer needs, staffing requirements, advisory boards, equipment inventory and capital budgets.
• Work with facility users and College faculty to identify and obtain new equipment as needed, typically through participation in the development of equipment grant proposals.
• Maintain facility equipment, negotiating and overseeing service contracts and equipment repairs as needed. Provide the College with an annual plan for equipment upkeep, rotation and the purchase of new equipment.
• Supervise facility staff and recommending staffing changes as needed.
• Oversee and manage the daily operations of all research user facilities.
• Assist the Associate Dean for Research and Graduate Affairs in the review of faculty requests for major equipment purchases and the transition (sale, movement, retirement) of any facility or faculty research equipment within the College.
• Assist the Associate Dean for Research Administration and Policy with the review and approval of Facility Use Agreements with industry or other outside entities.
Employee Class: Academic Staff Department: College of Engineering DeanÂ’s Office Full Time salary rate: Minimum $90,000 Annual (12 month). Appointment Percent: 100% Anticipated begin date: September 1, 2014 TO ENSURE CONSIDERATION
Application must be received by: JULY 01, 2014 HOW TO APPLY: Please submit a cover letter and resume or CV to Patricia Droes at: pdroes-at-engr.wisc.edu Unless another application procedure has been specified above, please send resume and cover letter referring to Position Vacancy Listing #79754 to: Susan Hagness Phone: 608-265-5739
1415 Engineering Dr Fax: N/A
2620 Engineering Hall Email: hagness-at-engr.wisc.edu
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Title-Subject: [Filtered] Artist project video on Chromosomes assistance needed
Message: Hi, I'm a working artist in Milwaukee, WI and am working on a project specifically about gender. I'm looking to do a side-by-side video piece, 1 showing XX and 1 showing XY chromosomes. I'm not a scientist nor do I have any understanding of what's involved! I do have a vague understanding that it will be with an electron microscope, the slides would be of human dyed dead cells since, i believe, when you show dna/chromosomes you have to break them apart or something. So I am specifically looking to find someone with the expertise to assist me in understanding what the process takes, what i'd exactly be looking at with the final video, and then to do the actual preparing of slides and filming. If all possible, I'd also love to be in attendance to see the process so I could learn and talk about the final piece with much more understanding. I know there might be stock footage out there to use but i'd prefer first to see if I can be apart of actually doing it as it helps me learn and have a much better understand and that is important to me as an artist.
Can anyone help me in this endeavor? Or point me to someone that can? The work will be showing at UW-Parkside in Wisconsin in November and will be comprised of photographic portraits of people, several video pieces and an installation piece. I can pay for any supplies but this is all personally funded so it would have to be reasonable. Thank you in advance for any assistance or help with this matter.
Thanks, Lois Bielefeld
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Jun 27 07:26:44 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RCQctk002049 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 07:26:42 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 4C62A7214B2 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 07:26:38 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id kGcVXn3aIuLx for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 27 Jun 2014 07:26:33 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id C05177214A5 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 07:26:33 -0500 (CDT) 14, 28 -- Message-ID: {53AD62F9.6000605-at-microscopy.com} 14, 28 -- Date: Fri, 27 Jun 2014 07:26:33 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Artist project video on Chromosomes assistance needed 14, 28 -- References: {201406261941.s5QJfWeH021180-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201406261941.s5QJfWeH021180-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201406261941.s5QJfWeH021180-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We had Perspex canopies made up for our SEMs, Auger system and SIMS machines to protect from direct sprinkler spray.
Regards, Barry
Barry Lamb Director Mead Testing Ltd Registered address: 25 Mead Park,River Way, Harlow, Essex, CM20 2SE, UK Company no. 4572600 barrylamb-at-meadtest.com www.meadtest.com tel: + 44 (0) 1279 635864 fax: +44 (0) 1279 635874
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: 27 June 2014 13:21 To: barrylamb-at-meadtest.com
Hi Rosemary
Out of interest I have dealt with water cascading over microscopes, yes it has happened twice in my 49 electron microscopy years. As a service technician the main problem is not so much the water, but the debris that the water beings down onto the electrical circuits. Both of the instruments in question were running on vacuum only and managed to keep going overnight until security found the water running through the particular laboratories. One was a tap exploding, shooting a fountain of water into the EM room, the other the result of putting out a fire three levels above.
A third situation occurred when a water pipe came off one instrument in a basement EM unit, flooding the complete area to about 6 inches. Everyone of the instruments kept going, but as I arrived a technician with rubber boots and a long wooden pole was taking care of switching the most endangered instruments off. However this was in the 1980s when most EM Units were underground; the one I mentioned was also used as a Tornado shelter!
EM Units and water, great fun!
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Sent: 26 June 2014 11:09 To: protrain-at-emcourses.com
Hi Everyone, Purdue University has an opening for an electron microscopy staff scientist in the Birck Nanotechnology Center.
Details can be found on the openings web site http://purdue.taleo.net/careersection/wl/jobdetail.ftl. The job number is 1401687
I can answer any general questions. If interested please apply immediately as the review process is about to begin.
Regards
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Interim Director, Biological Sciences Electron Microscope Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu http://www3.ag.purdue.edu/facilities/microscopy/Pages/default.aspx https://www.bio.purdue.edu/molecular_biosciences/microscope_facility.html
==============================Original Headers============================== 11, 29 -- From gilpin-at-purdue.edu Fri Jun 27 09:53:47 2014 11, 29 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RErkDi031597 11, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 09:53:46 -0500 11, 29 -- Received: from WPVEXCHUB03.purdue.lcl (wpvexchub03.itap.purdue.edu [172.30.136.67]) 11, 29 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s5RErkwn012488 11, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 10:53:46 -0400 11, 29 -- Received: from WPVEXCMBX04.purdue.lcl ([169.254.4.146]) by 11, 29 -- WPVEXCHUB03.purdue.lcl ([172.30.136.67]) with mapi id 14.03.0174.001; Fri, 27 11, 29 -- Jun 2014 10:53:46 -0400 11, 29 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 11, 29 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 11, 29 -- Subject: Staff Scientist Opening 11, 29 -- Thread-Topic: Staff Scientist Opening 11, 29 -- Thread-Index: Ac+SC3S/kcyoeyvaSzy7V2PU85WV/w== 11, 29 -- Date: Fri, 27 Jun 2014 14:53:46 +0000 11, 29 -- Message-ID: {57783A4A7CBA0840B44A39575397874E1CF69428-at-WPVEXCMBX04.purdue.lcl} 11, 29 -- Accept-Language: en-US 11, 29 -- Content-Language: en-US 11, 29 -- X-MS-Has-Attach: 11, 29 -- X-MS-TNEF-Correlator: 11, 29 -- x-originating-ip: [128.210.42.25] 11, 29 -- Content-Type: text/plain; charset="us-ascii" 11, 29 -- MIME-Version: 1.0 11, 29 -- X-PMX-Version: 6.0.2.2308539 11, 29 -- X-PerlMx-URL-Scanned: Yes 11, 29 -- X-PerlMx-Virus-Scanned: Yes 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5RErkDi031597 ==============================End of - Headers==============================
My question is is: “How many people have experienced sparkler systems go off in their microscope rooms?” Anyone want to jump in a relate experiences?
____ John Mansfield PhD Cphys MInstP *****Note New Business Mailing Address, all other information remains the same***** Microscopy Society of America - President-Elect Associate Director North Campus Electron Microbeam Analysis Laboratory Building 22, Room G010, University of Michigan 2800 Plymouth Road, Ann Arbor MI 48109-2800 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/ iMessage: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096
On Jun 27, 2014, at 9:17 AM, barrylamb-at-meadtest.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Rosemary, } } We had Perspex canopies made up for our SEMs, Auger system and SIMS } machines to protect from direct sprinkler spray. } } Regards, Barry } } Barry Lamb } Director } Mead Testing Ltd } Registered address: 25 Mead Park,River Way, Harlow, Essex, CM20 2SE, UK } Company no. 4572600 } barrylamb-at-meadtest.com } www.meadtest.com } tel: + 44 (0) 1279 635864 } fax: +44 (0) 1279 635874 } } } -----Original Message----- } X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] } Sent: 27 June 2014 13:21 } To: barrylamb-at-meadtest.com } Subject: [Microscopy] RE: Sprinklers in instrument rooms } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Hi Rosemary } } Out of interest I have dealt with water cascading over microscopes, yes } it } has happened twice in my 49 electron microscopy years. As a service } technician the main problem is not so much the water, but the debris } that } the water beings down onto the electrical circuits. Both of the } instruments } in question were running on vacuum only and managed to keep going } overnight } until security found the water running through the particular } laboratories. } One was a tap exploding, shooting a fountain of water into the EM room, } the } other the result of putting out a fire three levels above. } } A third situation occurred when a water pipe came off one instrument in } a } basement EM unit, flooding the complete area to about 6 inches. Everyone } of } the instruments kept going, but as I arrived a technician with rubber } boots } and a long wooden pole was taking care of switching the most endangered } instruments off. However this was in the 1980s when most EM Units were } underground; the one I mentioned was also used as a Tornado shelter! } } EM Units and water, great fun! } } Steve } } Steve Chapman FRMS } Protrain for Consultancy and Courses in Electron Microscopy } Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 } www.emcourses.com } } -----Original Message----- } X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] } Sent: 26 June 2014 11:09 } To: protrain-at-emcourses.com } Subject: [Microscopy] Sprinklers in instrument rooms } } Hi all, } } I know this has been discussed before, just wondered if there were any } new } thoughts. We're moving twice, first into a refurbished basement, second } time } into a new building. The basement has a sprinkler system in case of } fire, } unlike our current building which just has alarms (it's a single storey } brick building). I imagine the new building will have some sort of } sprinkler } system too. } } What precautions, if any, do people take to protect against the unlikely } showering of your confocal or EM? } Are there any relatively simple alternatives? (One alternative is to } have } fire-doors and walls for each room but that is prohibitive.) } } thanks much, } Rosemary } } Dr Rosemary White } CSIRO Plant Industry (for 4 more days only) GPO Box 1600 Canberra, ACT } 2601 } } T 02 6246 5475 } F 02 6246 5334 } E rosemary.white-at-csiro.au } } } } ==============================Original } Headers============================== } 8, 42 -- From prvs=247c2aa8e=Rosemary.White-at-csiro.au Thu Jun 26 05:06:26 } 2014 8, 42 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au } [150.229.7.43]) } 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id } s5QA6PA5015134 } 8, 42 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2014 } 05:06:26 } -0500 } 8, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 8, 42 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; } 8, 42 -- t=1403777186; x=1435313186; } 8, 42 -- h=from:to:subject:date:message-id:content-id: } 8, 42 -- content-transfer-encoding:mime-version; } 8, 42 -- bh=VIbpKTee+LuhFhXfdAd1Lbb3tIc9yz4Xf48uAzBQy9o=; } 8, 42 -- b=s+fmX214qQFQLuOIYZtoX3vpFy8FgxNKkXYI6ZJkrfKgNEF+aaoz7pIs } 8, 42 -- L5479FyaZz47npUsg7TsFiPs65fQVKkO1/LNn/nyxPIERJ0iA+k7USqDN } 8, 42 -- IWvLJtVNZQfA6ey; } 8, 42 -- X-SBRS: 5.1 } 8, 42 -- X-IronPort-Anti-Spam-Filtered: true 8, 42 -- } X-IronPort-Anti-Spam-Result: } A+DtCwBU8KtTlwCwBSSwhIATAJKcgCRagmmBUMItgmAWDwEBAQEBCBYHPIQKOlEBPkInBBOI } QgGW } D6x4F48dhC0FmliXMYIw } 8, 42 -- X-IPAS-Result: } A+DtCwBU8KtTlwCwBSSwhIATAJKcgCRagmmBUMItgmAWDwEBAQEBCBYHPIQKOlEBPkInBBOI } QgGW } D6x4F48dhC0FmliXMYIw } 8, 42 -- X-IronPort-AV: E=Sophos;i="5.01,552,1399989600"; } 8, 42 -- d="scan'208";a="125054031" } 8, 42 -- Received: from exhtca04-cdc.nexus.csiro.au } ([IPv6:2405:b000:601:13::247:24]) } 8, 42 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES128-SHA; 26 } Jun } 2014 20:06:24 +1000 } 8, 42 -- Received: from EXMBX04-CDC.nexus.csiro.au } ([fe80::9de3:d7af:4ed1:b7b6]) by 8, 42 -- exhtca04-cdc.nexus.csiro.au } ([fe80::7414:eafc:a0d3:3063%13]) with mapi id 8, 42 -- 14.03.0158.001; } Thu, } 26 Jun 2014 20:06:24 +1000 8, 42 -- From: {Rosemary.White-at-csiro.au} 8, } 42 -- } To: {microscopy-at-microscopy.com} 8, 42 -- Subject: Sprinklers in } instrument } rooms 8, 42 -- Thread-Topic: Sprinklers in instrument rooms 8, 42 -- } Thread-Index: AQHPkSZJS6Nnzyp0JEOC/Quml5SBXQ== 8, 42 -- Date: Thu, 26 } Jun } 2014 10:06:23 +0000 8, 42 -- Message-ID: } {CFD22DB3.162AD7%rosemary.white-at-csiro.au} } 8, 42 -- Accept-Language: en-NZ, en-US, en-AU 8, 42 -- Content-Language: } en-US 8, 42 -- X-MS-Has-Attach: } 8, 42 -- X-MS-TNEF-Correlator: } 8, 42 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 } 8, 42 -- x-originating-ip: [152.83.195.117] 8, 42 -- Content-Type: } text/plain; charset="us-ascii" } 8, 42 -- Content-ID: {68FD640B3B7F74469F89A8E68A9A2AA0-at-csiro.au} } 8, 42 -- MIME-Version: 1.0 } 8, 42 -- Content-Transfer-Encoding: 8bit 8, 42 -- X-MIME-Autoconverted: } from } quoted-printable to 8bit by ns.microscopy.com id s5QA6PA5015134 } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 17, 24 -- From protrain-at-emcourses.com Fri Jun 27 06:57:02 2014 } 17, 24 -- Received: from mail.esentra.net (mail.esentra.net } [94.228.36.208]) } 17, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id s5RBv1W9013530 } 17, 24 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 } 06:57:01 -0500 } 17, 24 -- Received: from mail.clientmail.net (Not } Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway } 17, 24 -- id {B53ad5c0b0000} ; Fri, 27 Jun 2014 12:56:59 +0100 } 17, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Fri, 27 } Jun 2014 12:56:59 +0100 } 17, 24 -- Reply-To: {protrain-at-emcourses.com} } 17, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} } 17, 24 -- To: {Rosemary.White-at-csiro.au} } 17, 24 -- Cc: "'Microscopical Soc of America'" } {microscopy-at-microscopy.com} } 17, 24 -- References: {201406261008.s5QA8we9016203-at-ns.microscopy.com} } 17, 24 -- In-Reply-To: {201406261008.s5QA8we9016203-at-ns.microscopy.com} } 17, 24 -- Subject: RE: [Microscopy] Sprinklers in instrument rooms } 17, 24 -- Date: Fri, 27 Jun 2014 12:56:58 +0100 } 17, 24 -- Organization: Protrain } 17, 24 -- Message-ID: {02cc01cf91fe$e74f2c40$b5ed84c0$-at-com} } 17, 24 -- MIME-Version: 1.0 } 17, 24 -- Content-Type: text/plain; } 17, 24 -- charset="US-ASCII" } 17, 24 -- Content-Transfer-Encoding: 7bit } 17, 24 -- X-Mailer: Microsoft Office Outlook 12.0 } 17, 24 -- Thread-Index: Ac+RJp0/CFZ+c0nrSTavJrHi2ztSYAA1aOUA } 17, 24 -- Content-Language: en-gb } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 28, 25 -- From barrylamb-at-meadtest.com Fri Jun 27 08:09:19 2014 } 28, 25 -- Received: from tdns.1stdnsltd.co.uk (tdns.1stdnsltd.co.uk [213.146.186.53] (may be forged)) } 28, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RD9I0N009144 } 28, 25 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 08:09:18 -0500 } 28, 25 -- Received: from Barry (host217-44-102-18.range217-44.btcentralplus.com [217.44.102.18]) } 28, 25 -- by tdns.1stdnsltd.co.uk (Postfix) with ESMTPA id B2C276008D; } 28, 25 -- Fri, 27 Jun 2014 14:09:17 +0100 (BST) } 28, 25 -- From: "Barry Lamb" {barrylamb-at-meadtest.com} } 28, 25 -- To: {Rosemary.White-at-csiro.au} } 28, 25 -- Cc: {microscopy-at-microscopy.com} } 28, 25 -- Subject: RE: [Microscopy] RE: Sprinklers in instrument rooms } 28, 25 -- Date: Fri, 27 Jun 2014 14:07:42 +0100 } 28, 25 -- Message-ID: {003801cf9208$c8340600$0c00a8c0-at-Barry} } 28, 25 -- MIME-Version: 1.0 } 28, 25 -- Content-Type: text/plain; } 28, 25 -- charset="us-ascii" } 28, 25 -- Content-Transfer-Encoding: 7bit } 28, 25 -- X-Priority: 3 (Normal) } 28, 25 -- X-MSMail-Priority: Normal } 28, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.2616 } 28, 25 -- Importance: Normal } 28, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3664 } 28, 25 -- In-Reply-To: {201406271221.s5RCLBg2027880-at-ns.microscopy.com} } 28, 25 -- X-PPP-Message-ID: {20140627130918.9904.55541-at-tdns.1stdnsltd.co.uk} } 28, 25 -- X-PPP-Vhost: meadtest.com } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 37 -- From jfmjfm-at-umich.edu Fri Jun 27 10:54:10 2014 8, 37 -- Received: from mail-wg0-f50.google.com (mail-wg0-f50.google.com [74.125.82.50]) 8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RFs9vG019144 8, 37 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 10:54:09 -0500 8, 37 -- Received: by mail-wg0-f50.google.com with SMTP id m15so5425503wgh.9 8, 37 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 08:54:08 -0700 (PDT) 8, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 37 -- d=1e100.net; s=20130820; 8, 37 -- h=x-gm-message-state:content-type:mime-version:subject:from 8, 37 -- :in-reply-to:date:content-transfer-encoding:message-id:references:to; 8, 37 -- bh=WQ5Rx2Wsx2+GAF+A6iXAQLAMXu+Ce/NARPsm0nfNn6I=; 8, 37 -- b=nDPd7NFivwZx15DY/l7YWoB8iO8REvzgxtLrsSQODKyqP4Z1LavVbGP62HIpIKGzM0 8, 37 -- gLasmRdZhJ3aT0Q71RyeBOWYmu6odZPeK6zMxT7DSp1+xYc3sgXVhR5i3JgM8VPE984/ 8, 37 -- SsQpyTSqzMst1tNr6f3GSJLYvXUxypu+Cr/K5oSNYE7OPwNKy6WVOJPemO5tWvmF0QAd 8, 37 -- q8jsXXMKOofEeACYfvnCSNwCN+kmdZhOZSa7ShiC2i5BKhCCde94Zet8KSdvEgyCjo8w 8, 37 -- VRrX7pe77JTwxcmK45BF1oeEcJQmpZzzTEmtItqu8Rbu4rtROgOLOyDDr4AfuT2TBODC 8, 37 -- i9fg== 8, 37 -- X-Gm-Message-State: ALoCoQkZUgG651fI/l8g8tD7X0e48aGHRA7GsC2c22Q0kqJ7ND66tYboVWYSNme+jP+hAJnF8FKm 8, 37 -- X-Received: by 10.194.89.168 with SMTP id bp8mr26892744wjb.73.1403884448612; 8, 37 -- Fri, 27 Jun 2014 08:54:08 -0700 (PDT) 8, 37 -- Received: from host-98.subnet-108.med.umich.edu (host-98.subnet-108.med.umich.edu. [141.214.108.98]) 8, 37 -- by mx.google.com with ESMTPSA id e8sm35133192wiz.9.2014.06.27.08.54.06 8, 37 -- for {multiple recipients} 8, 37 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 8, 37 -- Fri, 27 Jun 2014 08:54:07 -0700 (PDT) 8, 37 -- Content-Type: text/plain; charset=windows-1252 8, 37 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.2\)) 8, 37 -- Subject: Re: [Microscopy] Sprinklers in instrument rooms 8, 37 -- From: John Mansfield {jfmjfm-at-umich.edu} 8, 37 -- In-Reply-To: {201406271317.s5RDH02h020872-at-ns.microscopy.com} 8, 37 -- Date: Fri, 27 Jun 2014 11:54:04 -0400 8, 37 -- Message-Id: {E4168074-B60B-41FC-8310-CA64768CC081-at-umich.edu} 8, 37 -- References: {201406271317.s5RDH02h020872-at-ns.microscopy.com} 8, 37 -- To: microscopy-at-microscopy.com 8, 37 -- X-Mailer: Apple Mail (2.1878.2) 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5RFs9vG019144 ==============================End of - Headers==============================
As hinted at from my post on the confocal listserver, I worked in an E. M. lab years ago in a hospital with a sprinkler head that developed a drip. fortunately, the head wasn't over the microscope, and was swapped out. In my new lab, the engineers and construction crew put a sprinkler head directly above my TEM column, which I made them move to the left wall of my microscope suite.
Ed
Edward Haller, Lab Manager University of South Florida Department of Integrative Biology Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 (813)974-2676 ehaller-at-usf.edu Office: ISA 1046 http://biology.usf.edu/ib/research/facilities/
________________________________________ X-from: jfmjfm-at-umich.edu [jfmjfm-at-umich.edu] Sent: Friday, June 27, 2014 12:01 PM To: Haller, Edward
My question is is: “How many people have experienced sparkler systems go off in their microscope rooms?” Anyone want to jump in a relate experiences?
____ John Mansfield PhD Cphys MInstP *****Note New Business Mailing Address, all other information remains the same***** Microscopy Society of America - President-Elect Associate Director North Campus Electron Microbeam Analysis Laboratory Building 22, Room G010, University of Michigan 2800 Plymouth Road, Ann Arbor MI 48109-2800 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/ iMessage: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096
On Jun 27, 2014, at 9:17 AM, barrylamb-at-meadtest.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Rosemary, } } We had Perspex canopies made up for our SEMs, Auger system and SIMS } machines to protect from direct sprinkler spray. } } Regards, Barry } } Barry Lamb } Director } Mead Testing Ltd } Registered address: 25 Mead Park,River Way, Harlow, Essex, CM20 2SE, UK } Company no. 4572600 } barrylamb-at-meadtest.com } www.meadtest.com } tel: + 44 (0) 1279 635864 } fax: +44 (0) 1279 635874 } } } -----Original Message----- } X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] } Sent: 27 June 2014 13:21 } To: barrylamb-at-meadtest.com } Subject: [Microscopy] RE: Sprinklers in instrument rooms } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Hi Rosemary } } Out of interest I have dealt with water cascading over microscopes, yes } it } has happened twice in my 49 electron microscopy years. As a service } technician the main problem is not so much the water, but the debris } that } the water beings down onto the electrical circuits. Both of the } instruments } in question were running on vacuum only and managed to keep going } overnight } until security found the water running through the particular } laboratories. } One was a tap exploding, shooting a fountain of water into the EM room, } the } other the result of putting out a fire three levels above. } } A third situation occurred when a water pipe came off one instrument in } a } basement EM unit, flooding the complete area to about 6 inches. Everyone } of } the instruments kept going, but as I arrived a technician with rubber } boots } and a long wooden pole was taking care of switching the most endangered } instruments off. However this was in the 1980s when most EM Units were } underground; the one I mentioned was also used as a Tornado shelter! } } EM Units and water, great fun! } } Steve } } Steve Chapman FRMS } Protrain for Consultancy and Courses in Electron Microscopy } Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 } www.emcourses.com } } -----Original Message----- } X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] } Sent: 26 June 2014 11:09 } To: protrain-at-emcourses.com } Subject: [Microscopy] Sprinklers in instrument rooms } } Hi all, } } I know this has been discussed before, just wondered if there were any } new } thoughts. We're moving twice, first into a refurbished basement, second } time } into a new building. The basement has a sprinkler system in case of } fire, } unlike our current building which just has alarms (it's a single storey } brick building). I imagine the new building will have some sort of } sprinkler } system too. } } What precautions, if any, do people take to protect against the unlikely } showering of your confocal or EM? } Are there any relatively simple alternatives? (One alternative is to } have } fire-doors and walls for each room but that is prohibitive.) } } thanks much, } Rosemary } } Dr Rosemary White } CSIRO Plant Industry (for 4 more days only) GPO Box 1600 Canberra, ACT } 2601 } } T 02 6246 5475 } F 02 6246 5334 } E rosemary.white-at-csiro.au } } } } ==============================Original } Headers============================== } 8, 42 -- From prvs=247c2aa8e=Rosemary.White-at-csiro.au Thu Jun 26 05:06:26 } 2014 8, 42 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au } [150.229.7.43]) } 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id } s5QA6PA5015134 } 8, 42 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2014 } 05:06:26 } -0500 } 8, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 8, 42 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; } 8, 42 -- t=1403777186; x=1435313186; } 8, 42 -- h=from:to:subject:date:message-id:content-id: } 8, 42 -- content-transfer-encoding:mime-version; } 8, 42 -- bh=VIbpKTee+LuhFhXfdAd1Lbb3tIc9yz4Xf48uAzBQy9o=; 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charset="us-ascii" } 8, 42 -- Content-ID: {68FD640B3B7F74469F89A8E68A9A2AA0-at-csiro.au} } 8, 42 -- MIME-Version: 1.0 } 8, 42 -- Content-Transfer-Encoding: 8bit 8, 42 -- X-MIME-Autoconverted: } from } quoted-printable to 8bit by ns.microscopy.com id s5QA6PA5015134 } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 17, 24 -- From protrain-at-emcourses.com Fri Jun 27 06:57:02 2014 } 17, 24 -- Received: from mail.esentra.net (mail.esentra.net } [94.228.36.208]) } 17, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id s5RBv1W9013530 } 17, 24 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 } 06:57:01 -0500 } 17, 24 -- Received: from mail.clientmail.net (Not } Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway } 17, 24 -- id {B53ad5c0b0000} ; Fri, 27 Jun 2014 12:56:59 +0100 } 17, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Fri, 27 } Jun 2014 12:56:59 +0100 } 17, 24 -- Reply-To: {protrain-at-emcourses.com} } 17, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} } 17, 24 -- To: {Rosemary.White-at-csiro.au} } 17, 24 -- Cc: "'Microscopical Soc of America'" } {microscopy-at-microscopy.com} } 17, 24 -- References: {201406261008.s5QA8we9016203-at-ns.microscopy.com} } 17, 24 -- In-Reply-To: {201406261008.s5QA8we9016203-at-ns.microscopy.com} } 17, 24 -- Subject: RE: [Microscopy] Sprinklers in instrument rooms } 17, 24 -- Date: Fri, 27 Jun 2014 12:56:58 +0100 } 17, 24 -- Organization: Protrain } 17, 24 -- Message-ID: {02cc01cf91fe$e74f2c40$b5ed84c0$-at-com} } 17, 24 -- MIME-Version: 1.0 } 17, 24 -- Content-Type: text/plain; } 17, 24 -- charset="US-ASCII" } 17, 24 -- Content-Transfer-Encoding: 7bit } 17, 24 -- X-Mailer: Microsoft Office Outlook 12.0 } 17, 24 -- Thread-Index: Ac+RJp0/CFZ+c0nrSTavJrHi2ztSYAA1aOUA } 17, 24 -- Content-Language: en-gb } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 28, 25 -- From barrylamb-at-meadtest.com Fri Jun 27 08:09:19 2014 } 28, 25 -- Received: from tdns.1stdnsltd.co.uk (tdns.1stdnsltd.co.uk [213.146.186.53] (may be forged)) } 28, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RD9I0N009144 } 28, 25 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 08:09:18 -0500 } 28, 25 -- Received: from Barry (host217-44-102-18.range217-44.btcentralplus.com [217.44.102.18]) } 28, 25 -- by tdns.1stdnsltd.co.uk (Postfix) with ESMTPA id B2C276008D; } 28, 25 -- Fri, 27 Jun 2014 14:09:17 +0100 (BST) } 28, 25 -- From: "Barry Lamb" {barrylamb-at-meadtest.com} } 28, 25 -- To: {Rosemary.White-at-csiro.au} } 28, 25 -- Cc: {microscopy-at-microscopy.com} } 28, 25 -- Subject: RE: [Microscopy] RE: Sprinklers in instrument rooms } 28, 25 -- Date: Fri, 27 Jun 2014 14:07:42 +0100 } 28, 25 -- Message-ID: {003801cf9208$c8340600$0c00a8c0-at-Barry} } 28, 25 -- MIME-Version: 1.0 } 28, 25 -- Content-Type: text/plain; } 28, 25 -- charset="us-ascii" } 28, 25 -- Content-Transfer-Encoding: 7bit } 28, 25 -- X-Priority: 3 (Normal) } 28, 25 -- X-MSMail-Priority: Normal } 28, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.2616 } 28, 25 -- Importance: Normal } 28, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3664 } 28, 25 -- In-Reply-To: {201406271221.s5RCLBg2027880-at-ns.microscopy.com} } 28, 25 -- X-PPP-Message-ID: {20140627130918.9904.55541-at-tdns.1stdnsltd.co.uk} } 28, 25 -- X-PPP-Vhost: meadtest.com } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 37 -- From jfmjfm-at-umich.edu Fri Jun 27 10:54:10 2014 8, 37 -- Received: from mail-wg0-f50.google.com (mail-wg0-f50.google.com [74.125.82.50]) 8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RFs9vG019144 8, 37 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 10:54:09 -0500 8, 37 -- Received: by mail-wg0-f50.google.com with SMTP id m15so5425503wgh.9 8, 37 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 08:54:08 -0700 (PDT) 8, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 37 -- d=1e100.net; s=20130820; 8, 37 -- h=x-gm-message-state:content-type:mime-version:subject:from 8, 37 -- :in-reply-to:date:content-transfer-encoding:message-id:references:to; 8, 37 -- bh=WQ5Rx2Wsx2+GAF+A6iXAQLAMXu+Ce/NARPsm0nfNn6I=; 8, 37 -- b=nDPd7NFivwZx15DY/l7YWoB8iO8REvzgxtLrsSQODKyqP4Z1LavVbGP62HIpIKGzM0 8, 37 -- gLasmRdZhJ3aT0Q71RyeBOWYmu6odZPeK6zMxT7DSp1+xYc3sgXVhR5i3JgM8VPE984/ 8, 37 -- SsQpyTSqzMst1tNr6f3GSJLYvXUxypu+Cr/K5oSNYE7OPwNKy6WVOJPemO5tWvmF0QAd 8, 37 -- q8jsXXMKOofEeACYfvnCSNwCN+kmdZhOZSa7ShiC2i5BKhCCde94Zet8KSdvEgyCjo8w 8, 37 -- VRrX7pe77JTwxcmK45BF1oeEcJQmpZzzTEmtItqu8Rbu4rtROgOLOyDDr4AfuT2TBODC 8, 37 -- i9fg== 8, 37 -- X-Gm-Message-State: ALoCoQkZUgG651fI/l8g8tD7X0e48aGHRA7GsC2c22Q0kqJ7ND66tYboVWYSNme+jP+hAJnF8FKm 8, 37 -- X-Received: by 10.194.89.168 with SMTP id bp8mr26892744wjb.73.1403884448612; 8, 37 -- Fri, 27 Jun 2014 08:54:08 -0700 (PDT) 8, 37 -- Received: from host-98.subnet-108.med.umich.edu (host-98.subnet-108.med.umich.edu. [141.214.108.98]) 8, 37 -- by mx.google.com with ESMTPSA id e8sm35133192wiz.9.2014.06.27.08.54.06 8, 37 -- for {multiple recipients} 8, 37 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 8, 37 -- Fri, 27 Jun 2014 08:54:07 -0700 (PDT) 8, 37 -- Content-Type: text/plain; charset=windows-1252 8, 37 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.2\)) 8, 37 -- Subject: Re: [Microscopy] Sprinklers in instrument rooms 8, 37 -- From: John Mansfield {jfmjfm-at-umich.edu} 8, 37 -- In-Reply-To: {201406271317.s5RDH02h020872-at-ns.microscopy.com} 8, 37 -- Date: Fri, 27 Jun 2014 11:54:04 -0400 8, 37 -- Message-Id: {E4168074-B60B-41FC-8310-CA64768CC081-at-umich.edu} 8, 37 -- References: {201406271317.s5RDH02h020872-at-ns.microscopy.com} 8, 37 -- To: microscopy-at-microscopy.com 8, 37 -- X-Mailer: Apple Mail (2.1878.2) 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5RFs9vG019144 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 47 -- From ehaller-at-health.usf.edu Fri Jun 27 11:44:10 2014 15, 47 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 15, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RGiAEM006427 15, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 11:44:10 -0500 15, 47 -- X-ASG-Debug-ID: 1403887445-045fa86a5f5ff80001-1DjkGe 15, 47 -- Received: from EXCH02.hscnet.hsc.usf.edu ([10.119.4.42]) by hscantispam.health.usf.edu with ESMTP id jqeyCAUfNleFY0Y9; Fri, 27 Jun 2014 12:44:05 -0400 (EDT) 15, 47 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 15, 47 -- Received: from SMTP1.hscnet.hsc.usf.edu (172.27.1.36) by 15, 47 -- EXCH02.hscnet.hsc.usf.edu (10.119.4.42) with Microsoft SMTP Server (TLS) id 15, 47 -- 14.3.181.6; Fri, 27 Jun 2014 12:44:05 -0400 15, 47 -- Received: from EXCH04.hscnet.hsc.usf.edu ([169.254.2.240]) by 15, 47 -- SMTP1.hscnet.hsc.usf.edu ([::1]) with mapi id 14.03.0181.006; Fri, 27 Jun 15, 47 -- 2014 12:44:05 -0400 15, 47 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 15, 47 -- To: "jfmjfm-at-umich.edu" {jfmjfm-at-umich.edu} , 15, 47 -- "Microscopy-at-microscopy.com" 15, 47 -- {Microscopy-at-microscopy.com} 15, 47 -- Subject: RE: [Microscopy] Re: Sprinklers in instrument rooms 15, 47 -- Thread-Topic: [Microscopy] Re: Sprinklers in instrument rooms 15, 47 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: Sprinklers in instrument rooms 15, 47 -- Thread-Index: AQHPkiEcaQb+i0YzTkWw5RhsZeyXhpuFKO2U 15, 47 -- Date: Fri, 27 Jun 2014 16:44:04 +0000 15, 47 -- Message-ID: {89AF5EEF4C4B8943A8D091D28859F7C898DC6E10-at-EXCH04.hscnet.hsc.usf.edu} 15, 47 -- References: {201406271601.s5RG1pCH029365-at-ns.microscopy.com} 15, 47 -- In-Reply-To: {201406271601.s5RG1pCH029365-at-ns.microscopy.com} 15, 47 -- Accept-Language: en-US 15, 47 -- Content-Language: en-US 15, 47 -- X-MS-Has-Attach: 15, 47 -- X-MS-TNEF-Correlator: 15, 47 -- x-originating-ip: [10.119.4.53] 15, 47 -- Content-Type: text/plain; charset="Windows-1252" 15, 47 -- MIME-Version: 1.0 15, 47 -- X-Barracuda-Connect: UNKNOWN[10.119.4.42] 15, 47 -- X-Barracuda-Start-Time: 1403887445 15, 47 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-mod/mark.cgi 15, 47 -- X-Virus-Scanned: by bsmtpd at health.usf.edu 15, 47 -- X-Barracuda-Spam-Score: 1.00 15, 47 -- X-Barracuda-Spam-Status: No, SCORE=1.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M, BSF_SC0_MISMATCH_TO, URI_NOVOWEL 15, 47 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.7000 15, 47 -- Rule breakdown below 15, 47 -- pts rule name description 15, 47 -- ---- ---------------------- -------------------------------------------------- 15, 47 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header 15, 47 -- 0.50 URI_NOVOWEL URI: URI hostname has long non-vowel sequence 15, 47 -- 0.50 BSF_RULE7568M Custom Rule 7568M 15, 47 -- Content-Transfer-Encoding: 8bit 15, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5RGiAEM006427 ==============================End of - Headers==============================
At a previous job of mine, we had a sprinkler head spontaneously fail catastrophically. The entire contents of the rooftop reservoir emptied into the space, which had offices and labs (fortunately "just" test equipment). Lots of water, and had a dispersion of fine black particulates in it. Apparently this built up from reactions of water with the pipes over long times. Very destructive.
-----Original Message----- X-from: ehaller-at-health.usf.edu [mailto:ehaller-at-health.usf.edu] Sent: Friday, June 27, 2014 12:55 PM To: LES-at-ZSGENETICS.COM
As hinted at from my post on the confocal listserver, I worked in an E. M. lab years ago in a hospital with a sprinkler head that developed a drip. fortunately, the head wasn't over the microscope, and was swapped out. In my new lab, the engineers and construction crew put a sprinkler head directly above my TEM column, which I made them move to the left wall of my microscope suite.
Ed
Edward Haller, Lab Manager University of South Florida Department of Integrative Biology Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 (813)974-2676 ehaller-at-usf.edu Office: ISA 1046 http://biology.usf.edu/ib/research/facilities/
________________________________________ X-from: jfmjfm-at-umich.edu [jfmjfm-at-umich.edu] Sent: Friday, June 27, 2014 12:01 PM To: Haller, Edward
My question is is: "How many people have experienced sparkler systems go off in their microscope rooms?" Anyone want to jump in a relate experiences?
____ John Mansfield PhD Cphys MInstP *****Note New Business Mailing Address, all other information remains the same***** Microscopy Society of America - President-Elect Associate Director North Campus Electron Microbeam Analysis Laboratory Building 22, Room G010, University of Michigan 2800 Plymouth Road, Ann Arbor MI 48109-2800 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/ iMessage: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096
On Jun 27, 2014, at 9:17 AM, barrylamb-at-meadtest.com wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Rosemary, } } We had Perspex canopies made up for our SEMs, Auger system and SIMS } machines to protect from direct sprinkler spray. } } Regards, Barry } } Barry Lamb } Director } Mead Testing Ltd } Registered address: 25 Mead Park,River Way, Harlow, Essex, CM20 2SE, } UK Company no. 4572600 barrylamb-at-meadtest.com www.meadtest.com } tel: + 44 (0) 1279 635864 } fax: +44 (0) 1279 635874 } } } -----Original Message----- } X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] } Sent: 27 June 2014 13:21 } To: barrylamb-at-meadtest.com } Subject: [Microscopy] RE: Sprinklers in instrument rooms } } } } } ---------------------------------------------------------------------- } -- } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -- } ---- } } Hi Rosemary } } Out of interest I have dealt with water cascading over microscopes, } yes it has happened twice in my 49 electron microscopy years. As a } service technician the main problem is not so much the water, but the } debris that the water beings down onto the electrical circuits. Both } of the instruments in question were running on vacuum only and managed } to keep going overnight until security found the water running through } the particular laboratories. } One was a tap exploding, shooting a fountain of water into the EM } room, the other the result of putting out a fire three levels above. } } A third situation occurred when a water pipe came off one instrument } in a basement EM unit, flooding the complete area to about 6 inches. } Everyone of the instruments kept going, but as I arrived a technician } with rubber boots and a long wooden pole was taking care of switching } the most endangered instruments off. However this was in the 1980s } when most EM Units were underground; the one I mentioned was also used } as a Tornado shelter! } } EM Units and water, great fun! } } Steve } } Steve Chapman FRMS } Protrain for Consultancy and Courses in Electron Microscopy } Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 } www.emcourses.com } } -----Original Message----- } X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] } Sent: 26 June 2014 11:09 } To: protrain-at-emcourses.com } Subject: [Microscopy] Sprinklers in instrument rooms } } Hi all, } } I know this has been discussed before, just wondered if there were any } new thoughts. We're moving twice, first into a refurbished basement, } second time into a new building. The basement has a sprinkler system } in case of fire, unlike our current building which just has alarms } (it's a single storey brick building). I imagine the new building will } have some sort of sprinkler system too. } } What precautions, if any, do people take to protect against the } unlikely showering of your confocal or EM? } Are there any relatively simple alternatives? (One alternative is to } have fire-doors and walls for each room but that is prohibitive.) } } thanks much, } Rosemary } } Dr Rosemary White } CSIRO Plant Industry (for 4 more days only) GPO Box 1600 Canberra, ACT } 2601 } } T 02 6246 5475 } F 02 6246 5334 } E rosemary.white-at-csiro.au } } } } ==============================Original } Headers============================== } 8, 42 -- From prvs=247c2aa8e=Rosemary.White-at-csiro.au Thu Jun 26 } 05:06:26 } 2014 8, 42 -- Received: from act-MTAout6.csiro.au } (act-MTAout6.csiro.au } [150.229.7.43]) } 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id } s5QA6PA5015134 } 8, 42 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2014 } 05:06:26 } -0500 } 8, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 8, 42 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; } 8, 42 -- t=1403777186; x=1435313186; } 8, 42 -- h=from:to:subject:date:message-id:content-id: } 8, 42 -- content-transfer-encoding:mime-version; } 8, 42 -- bh=VIbpKTee+LuhFhXfdAd1Lbb3tIc9yz4Xf48uAzBQy9o=; 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Thu, } 26 Jun 2014 20:06:24 +1000 8, 42 -- From: {Rosemary.White-at-csiro.au} 8, } 42 -- } To: {microscopy-at-microscopy.com} 8, 42 -- Subject: Sprinklers in } instrument rooms 8, 42 -- Thread-Topic: Sprinklers in instrument rooms } 8, 42 -- } Thread-Index: AQHPkSZJS6Nnzyp0JEOC/Quml5SBXQ== 8, 42 -- Date: Thu, 26 } Jun } 2014 10:06:23 +0000 8, 42 -- Message-ID: } {CFD22DB3.162AD7%rosemary.white-at-csiro.au} } 8, 42 -- Accept-Language: en-NZ, en-US, en-AU 8, 42 -- Content-Language: } en-US 8, 42 -- X-MS-Has-Attach: } 8, 42 -- X-MS-TNEF-Correlator: } 8, 42 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 } 8, 42 -- x-originating-ip: [152.83.195.117] 8, 42 -- Content-Type: } text/plain; charset="us-ascii" } 8, 42 -- Content-ID: {68FD640B3B7F74469F89A8E68A9A2AA0-at-csiro.au} } 8, 42 -- MIME-Version: 1.0 } 8, 42 -- Content-Transfer-Encoding: 8bit 8, 42 -- X-MIME-Autoconverted: } from } quoted-printable to 8bit by ns.microscopy.com id s5QA6PA5015134 } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 17, 24 -- From protrain-at-emcourses.com Fri Jun 27 06:57:02 2014 17, 24 } -- Received: from mail.esentra.net (mail.esentra.net } [94.228.36.208]) } 17, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id s5RBv1W9013530 } 17, 24 -- for {microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 15, 47 -- From ehaller-at-health.usf.edu Fri Jun 27 11:44:10 2014 15, 47 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 15, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RGiAEM006427 15, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 11:44:10 -0500 15, 47 -- X-ASG-Debug-ID: 1403887445-045fa86a5f5ff80001-1DjkGe 15, 47 -- Received: from EXCH02.hscnet.hsc.usf.edu ([10.119.4.42]) by hscantispam.health.usf.edu with ESMTP id jqeyCAUfNleFY0Y9; Fri, 27 Jun 2014 12:44:05 -0400 (EDT) 15, 47 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 15, 47 -- Received: from SMTP1.hscnet.hsc.usf.edu (172.27.1.36) by 15, 47 -- EXCH02.hscnet.hsc.usf.edu (10.119.4.42) with
I just love the Apple autocorrect! Sprinkler systems. I am willing to bet that NO-ONE has experienced an accidental release of water from a sprinkler system. Flooding from other labs or burst pipes, yes, but sprinklers I think not.
____ John Mansfield PhD Cphys MInstP *****Note New Business Mailing Address, all other information remains the same***** Microscopy Society of America - President-Elect Associate Director North Campus Electron Microbeam Analysis Laboratory Building 22, Room G010, University of Michigan 2800 Plymouth Road, Ann Arbor MI 48109-2800 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/ iMessage: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096
On Jun 27, 2014, at 12:26 PM, Philip Oshel {oshel1pe-at-cmich.edu} wrote:
} John, } } A very relevant question, given the 4th of July is next week, but I don't think many people have "sparkler systems" in their microscopy labs. } Would be pretty and exciting, though ... } } Phil } } } } My question is is: } } “How many people have experienced sparkler systems go off in their microscope rooms?” } } Anyone want to jump in a relate experiences? } } } } } } ____ } } John Mansfield PhD Cphys MInstP } } *****Note New Business Mailing Address, all other information remains the same***** } } Microscopy Society of America - President-Elect } } Associate Director } } North Campus Electron Microbeam Analysis Laboratory } } Building 22, Room G010, University of Michigan } } 2800 Plymouth Road, Ann Arbor MI 48109-2800 USA } } Phone: (734) 936-3352 FAX (734) 763-2282 } } Cell. Phone: (734) 834-3913 } } Email: jfmjfm-at-umich.edu } } URL: http://emalwww.engin.umich.edu/ } } iMessage: thejfmjfm } } Skype: thejfmjfm } } } } Home address: } } 4304 Spring Lake Boulevard } } Ann Arbor MI 48108-9657 } } Phone (734) 994-3096 } } } } On Jun 27, 2014, at 9:17 AM, barrylamb-at-meadtest.com wrote: } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Hi Rosemary, } } } } } } We had Perspex canopies made up for our SEMs, Auger system and SIMS } } } machines to protect from direct sprinkler spray. } } } } } } Regards, Barry } } } } } } Barry Lamb } } } Director } } } Mead Testing Ltd } } } Registered address: 25 Mead Park,River Way, Harlow, Essex, CM20 2SE, UK } } } Company no. 4572600 } } } barrylamb-at-meadtest.com } } } www.meadtest.com } } } tel: + 44 (0) 1279 635864 } } } fax: +44 (0) 1279 635874 } } } } } } } } } -----Original Message----- } } } X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] } } } Sent: 27 June 2014 13:21 } } } To: barrylamb-at-meadtest.com } } } Subject: [Microscopy] RE: Sprinklers in instrument rooms } } } } } } } } } } } } } } } ------------------------------------------------------------------------ } } } ---- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } } } ---- } } } } } } Hi Rosemary } } } } } } Out of interest I have dealt with water cascading over microscopes, yes } } } it } } } has happened twice in my 49 electron microscopy years. As a service } } } technician the main problem is not so much the water, but the debris } } } that } } } the water beings down onto the electrical circuits. Both of the } } } instruments } } } in question were running on vacuum only and managed to keep going } } } overnight } } } until security found the water running through the particular } } } laboratories. } } } One was a tap exploding, shooting a fountain of water into the EM room, } } } the } } } other the result of putting out a fire three levels above. } } } } } } A third situation occurred when a water pipe came off one instrument in } } } a } } } basement EM unit, flooding the complete area to about 6 inches. Everyone } } } of } } } the instruments kept going, but as I arrived a technician with rubber } } } boots } } } and a long wooden pole was taking care of switching the most endangered } } } instruments off. However this was in the 1980s when most EM Units were } } } underground; the one I mentioned was also used as a Tornado shelter! } } } } } } EM Units and water, great fun! } } } } } } Steve } } } } } } Steve Chapman FRMS } } } Protrain for Consultancy and Courses in Electron Microscopy } } } Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 } } } www.emcourses.com } } } } } } -----Original Message----- } } } X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] } } } Sent: 26 June 2014 11:09 } } } To: protrain-at-emcourses.com } } } Subject: [Microscopy] Sprinklers in instrument rooms } } } } } } Hi all, } } } } } } I know this has been discussed before, just wondered if there were any } } } new } } } thoughts. We're moving twice, first into a refurbished basement, second } } } time } } } into a new building. The basement has a sprinkler system in case of } } } fire, } } } unlike our current building which just has alarms (it's a single storey } } } brick building). I imagine the new building will have some sort of } } } sprinkler } } } system too. } } } } } } What precautions, if any, do people take to protect against the unlikely } } } showering of your confocal or EM? } } } Are there any relatively simple alternatives? (One alternative is to } } } have } } } fire-doors and walls for each room but that is prohibitive.) } } } } } } thanks much, } } } Rosemary } } } } } } Dr Rosemary White } } } CSIRO Plant Industry (for 4 more days only) GPO Box 1600 Canberra, ACT } } } 2601 } } } } } } T 02 6246 5475 } } } F 02 6246 5334 } } } E rosemary.white-at-csiro.au } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } 8, 42 -- From prvs=247c2aa8e=Rosemary.White-at-csiro.au Thu Jun 26 05:06:26 } } } 2014 8, 42 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au } } } [150.229.7.43]) } } } 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id } } } s5QA6PA5015134 } } } 8, 42 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2014 } } } 05:06:26 } } } -0500 } } } 8, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } } } 8, 42 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; } } } 8, 42 -- t=1403777186; x=1435313186; 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26 } } } Jun } } } 2014 20:06:24 +1000 } } } 8, 42 -- Received: from EXMBX04-CDC.nexus.csiro.au } } } ([fe80::9de3:d7af:4ed1:b7b6]) by 8, 42 -- exhtca04-cdc.nexus.csiro.au } } } ([fe80::7414:eafc:a0d3:3063%13]) with mapi id 8, 42 -- 14.03.0158.001; } } } Thu, } } } 26 Jun 2014 20:06:24 +1000 8, 42 -- From: {Rosemary.White-at-csiro.au} 8, } } } 42 -- } } } To: {microscopy-at-microscopy.com} 8, 42 -- Subject: Sprinklers in } } } instrument } } } rooms 8, 42 -- Thread-Topic: Sprinklers in instrument rooms 8, 42 -- } } } Thread-Index: AQHPkSZJS6Nnzyp0JEOC/Quml5SBXQ== 8, 42 -- Date: Thu, 26 } } } Jun } } } 2014 10:06:23 +0000 8, 42 -- Message-ID: } } } {CFD22DB3.162AD7%rosemary.white-at-csiro.au} } } } 8, 42 -- Accept-Language: en-NZ, en-US, en-AU 8, 42 -- Content-Language: } } } en-US 8, 42 -- X-MS-Has-Attach: } } } 8, 42 -- X-MS-TNEF-Correlator: } } } 8, 42 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 } } } 8, 42 -- x-originating-ip: [152.83.195.117] 8, 42 -- Content-Type: } } } text/plain; 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[141.214.108.98]) } } 8, 37 -- by mx.google.com with ESMTPSA id e8sm35133192wiz.9.2014.06.27.08.54.06 } } 8, 37 -- for {multiple recipients} } } 8, 37 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); } } 8, 37 -- Fri, 27 Jun 2014 08:54:07 -0700 (PDT) } } 8, 37 -- Content-Type: text/plain; charset=windows-1252 } } 8, 37 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.2\)) } } 8, 37 -- Subject: Re: [Microscopy] Sprinklers in instrument rooms } } 8, 37 -- From: John Mansfield {jfmjfm-at-umich.edu} } } 8, 37 -- In-Reply-To: {201406271317.s5RDH02h020872-at-ns.microscopy.com} } } 8, 37 -- Date: Fri, 27 Jun 2014 11:54:04 -0400 } } 8, 37 -- Message-Id: {E4168074-B60B-41FC-8310-CA64768CC081-at-umich.edu} } } 8, 37 -- References: {201406271317.s5RDH02h020872-at-ns.microscopy.com} } } 8, 37 -- To: microscopy-at-microscopy.com } } 8, 37 -- X-Mailer: Apple Mail (2.1878.2) } } 8, 37 -- Content-Transfer-Encoding: 8bit } } 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5RFs9vG019144 } } ==============================End of - Headers============================== } } . } } } } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } (989) 774-3576 }
==============================Original Headers============================== 7, 39 -- From jfmjfm-at-umich.edu Fri Jun 27 14:46:41 2014 7, 39 -- Received: from mail-wi0-f171.google.com (mail-wi0-f171.google.com [209.85.212.171]) 7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RJkeke015071 7, 39 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 14:46:40 -0500 7, 39 -- Received: by mail-wi0-f171.google.com with SMTP id n15so3382124wiw.4 7, 39 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 12:46:40 -0700 (PDT) 7, 39 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 39 -- d=1e100.net; s=20130820; 7, 39 -- h=x-gm-message-state:subject:mime-version:content-type:from 7, 39 -- :in-reply-to:date:cc:content-transfer-encoding:message-id:references 7, 39 -- :to; 7, 39 -- bh=qLQAkoPhkm+34gyUSYTcNFEeTDw5QpmrmrMyo+DuL4g=; 7, 39 -- b=kbisx7S2ud/+8EYkxPRzHdnY2tx7XoENwyZolDm3ZUfke0BOZbNPrrWJ3x7VZK7TEW 7, 39 -- 7JmwU5K2giAMUQp6SDG02gPAm1oXZCt3scA5/NVFX7V9oIVQcyuJgyGvqjcP0wqA1cBI 7, 39 -- kKwmcys5Spy9fIQqsdhgCvr9Z1bsrPeWILPqwDcI3zzt5niEzmxWEyqs8Q9n96DMA/zN 7, 39 -- gMJqW+oDsvm1jWOmfJuoe9GP+gjPwajZjy3SiEpoL6uKraYOkHmOt5azie27j4bpaads 7, 39 -- DdV//aTQPjQaTen6hwXaZHGgsQ+/S6Du+n1Iqi7yRVhoC+6ldmueLdkVy0FLf+mnyQlC 7, 39 -- nF0w== 7, 39 -- X-Gm-Message-State: ALoCoQnygKH2X8xcgtsGjo36DJEq0d2V+5gViV67PspZg7IxbHS5NCzxMtW4SVm72Ls2oRih65BG 7, 39 -- X-Received: by 10.180.20.112 with SMTP id m16mr14136707wie.6.1403898399960; 7, 39 -- Fri, 27 Jun 2014 12:46:39 -0700 (PDT) 7, 39 -- Received: from host-98.subnet-108.med.umich.edu (host-98.subnet-108.med.umich.edu. [141.214.108.98]) 7, 39 -- by mx.google.com with ESMTPSA id lk7sm23322568wjb.24.2014.06.27.12.46.38 7, 39 -- for {multiple recipients} 7, 39 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 7, 39 -- Fri, 27 Jun 2014 12:46:39 -0700 (PDT) 7, 39 -- Subject: Re: [Microscopy] Re: Sprinklers in instrument rooms 7, 39 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.2\)) 7, 39 -- Content-Type: text/plain; charset=windows-1252 7, 39 -- From: John Mansfield {jfmjfm-at-umich.edu} 7, 39 -- In-Reply-To: {53AD9B24.6070509-at-cmich.edu} 7, 39 -- Date: Fri, 27 Jun 2014 15:46:36 -0400 7, 39 -- Cc: microscopy-at-microscopy.com 7, 39 -- Message-Id: {8E3A2104-0F5D-4DEC-9E74-E32674CB1346-at-umich.edu} 7, 39 -- References: {201406271605.s5RG5veO004060-at-ns.microscopy.com} {53AD9B24.6070509-at-cmich.edu} 7, 39 -- To: oshel1pe-at-cmich.edu 7, 39 -- X-Mailer: Apple Mail (2.1878.2) 7, 39 -- Content-Transfer-Encoding: 8bit 7, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5RJkeke015071 ==============================End of - Headers==============================
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Interim Director, Biological Sciences Electron Microscope Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu http://www3.ag.purdue.edu/facilities/microscopy/Pages/default.aspx https://www.bio.purdue.edu/molecular_biosciences/microscope_facility.html
==============================Original Headers============================== 10, 29 -- From gilpin-at-purdue.edu Fri Jun 27 15:02:49 2014 10, 29 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RK2nl2001853 10, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 15:02:49 -0500 10, 29 -- Received: from WPVEXCHUB03.purdue.lcl (wpvexchub03.itap.purdue.edu [172.30.136.67]) 10, 29 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s5RK2ngJ032480 10, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 16:02:49 -0400 10, 29 -- Received: from WPVEXCMBX04.purdue.lcl ([169.254.4.146]) by 10, 29 -- WPVEXCHUB03.purdue.lcl ([172.30.136.67]) with mapi id 14.03.0174.001; Fri, 27 10, 29 -- Jun 2014 16:02:48 -0400 10, 29 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 10, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 29 -- Subject: Staff Scientist position open 10, 29 -- Thread-Topic: Staff Scientist position open 10, 29 -- Thread-Index: Ac+SQsP5YLNJ1SDYTZKGuR5NocRgMA== 10, 29 -- Date: Fri, 27 Jun 2014 20:02:48 +0000 10, 29 -- Message-ID: {57783A4A7CBA0840B44A39575397874E1CF69D4A-at-WPVEXCMBX04.purdue.lcl} 10, 29 -- Accept-Language: en-US 10, 29 -- Content-Language: en-US 10, 29 -- X-MS-Has-Attach: 10, 29 -- X-MS-TNEF-Correlator: 10, 29 -- x-originating-ip: [128.210.42.25] 10, 29 -- Content-Type: text/plain; charset="us-ascii" 10, 29 -- MIME-Version: 1.0 10, 29 -- X-PMX-Version: 6.0.2.2308539 10, 29 -- X-PerlMx-URL-Scanned: Yes 10, 29 -- X-PerlMx-Virus-Scanned: Yes 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5RK2nl2001853 ==============================End of - Headers==============================
Do you have experience with HPF-FS of petals from mature flowers? If so than I would appreciate your contacting me as I have some technical questions to ask you. We are primarily interested in the epithelial layer and this is a challenge since there is so little cytoplasm.
Thanks, Debby
Debra Sherman, Founder & CSO DS imaging LLC Purdue Technology Center 1281 Win Hentschel Blvd West Lafayette, IN 47906 E-mail: debby.sherman-at-dsimagingllc.com www.dsimagingllc.com Mobile: 765-418-8540
==============================Original Headers============================== 8, 31 -- From dsherman-at-purdue.edu Fri Jun 27 16:21:02 2014 8, 31 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5RLL1da021773 8, 31 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 16:21:02 -0500 8, 31 -- Received: from WPVEXCHUB01.purdue.lcl (wpvexchub01.itap.purdue.edu [172.30.136.61]) 8, 31 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s5RLL1NN022254 8, 31 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2014 17:21:01 -0400 8, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([169.254.2.103]) by 8, 31 -- WPVEXCHUB01.purdue.lcl ([172.30.136.61]) with mapi id 14.03.0174.001; Fri, 27 8, 31 -- Jun 2014 17:21:01 -0400 8, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 8, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 31 -- Subject: HPF of flower petals 8, 31 -- Thread-Topic: HPF of flower petals 8, 31 -- Thread-Index: AQHPkk2x8QYD2W1b8kKtsjlvcLv8+g== 8, 31 -- Date: Fri, 27 Jun 2014 21:21:00 +0000 8, 31 -- Message-ID: {CFD3587A.1F5B7%dsherman-at-purdue.edu} 8, 31 -- Accept-Language: en-US 8, 31 -- Content-Language: en-US 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- user-agent: Microsoft-MacOutlook/14.4.2.140509 8, 31 -- x-originating-ip: [24.14.170.252] 8, 31 -- Content-Type: text/plain; charset="us-ascii" 8, 31 -- Content-ID: {6A7298D875AF3E4587AD8976BC372BB7-at-exchange.purdue.edu} 8, 31 -- MIME-Version: 1.0 8, 31 -- X-PMX-Version: 6.0.2.2308539 8, 31 -- X-PerlMx-URL-Scanned: Yes 8, 31 -- X-PerlMx-Virus-Scanned: Yes 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5RLL1da021773 ==============================End of - Headers==============================
From pharmacy-popular11-at-charter.com Fri Jun 27 19:34:38 2014 Return-Path: {pharmacy-popular11-at-charter.com} Received: from 66-214-45-22.static.mtpk.ca.charter.com (66-214-45-22.static.mtpk.ca.charter.com [66.214.45.22]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5S0YYrg010149 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 27 Jun 2014 19:34:35 -0500 Message-Id: {201406280034.s5S0YYrg010149-at-ns.microscopy.com}
X-from: dan.fairweather-at-delphi.com ()
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Email: dan.fairweather-at-delphi.com Name: Dan Fairweather
Organization: Delphi Automotive, Powertrain Div
Title-Subject: [Filtered] S3200N spare parts?
Message: It looks like we need a new needle valve for our Hitachi S3200N. We have one on order with a promised delivery of late August. I was wondering if there is any one out there who might have something on the shelf, or maybe a "mildly" used one.
The Hitachi part number is 49E-7391.
Thank you for your assistance, Dan Fairweather Senior Materials Development Engineer Delphi Powertrain
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Jun 28 07:27:13 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5SCRCb5009473 13, 28 -- for {microscopy-at-microscopy.com} ; Sat, 28 Jun 2014 07:27:13 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id AF5EE721EB6 13, 28 -- for {microscopy-at-microscopy.com} ; Sat, 28 Jun 2014 07:27:11 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id VRHQpFE25fUO for {microscopy-at-microscopy.com} ; 13, 28 -- Sat, 28 Jun 2014 07:27:05 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 9B29A721EA4 13, 28 -- for {microscopy-at-microscopy.com} ; Sat, 28 Jun 2014 07:27:05 -0500 (CDT) 13, 28 -- Message-ID: {53AEB499.8060900-at-microscopy.com} 13, 28 -- Date: Sat, 28 Jun 2014 07:27:05 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:S3200N spare parts? 13, 28 -- References: {201406271541.s5RFfHBI018947-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201406271541.s5RFfHBI018947-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201406271541.s5RFfHBI018947-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] SEM - Need help with which scope to use for insects
Message: I am an undergraduate student at the University of Manitoba working on a revision of a genus of parasitic wasps. I have three new species that I would like to take images of in order to see their sculpturing, and I’m trying to figure out which SEM I should use. The specimens are about 3mm in length, and because they are not mine, I need them to remain as is (they have to be returned in the same condition in which I received them). The insects are point mounted, which means they are glued to the end of a small, pointed piece of paper which then has a pin stuck through it. The glue used likely dissolves in ethanol.
The two scopes we have here at the university are: Philips XL 30 and JEOL JSM-5900LV. I’m hoping someone could provide me with a recommendation on which to use, and advise me on what I will need to do if one of these scopes is suitable.
Any information would be of great help! Thank you,
Melanie Scallion
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: University of Connecticut
Title-Subject: [Filtered] Re: Sprinklers in instrument rooms
Message: Here is a situation that I bet not many have encountered: We had a TEM which developed a leak in lens cooling with the result that the INSIDE of the TEM filled with water. (I was not here but was told the camera/viewing chamber looked like "a fish bowl.")
My predecessor, Larry McCurdy, courageously attacked the mess and restored the TEM to service! It soldiered on for many years.
Point of story: Flooding the OUTSIDE of the TEM may actually be more harmful (electrical shorts,etc.) than flooding the INSIDE!
Cheers Roger
Institute of Materials Science University of Connecticut
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] beam fluctuation in FEI T20
Message: Hello,
Variation in beam intensity is observed with LaB6. How ever the LaB6 filament is showing the connectivity. What could be the possible problem.
Rashmi
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Melanie - I think you are going to have a tough time viewing these in an SEM in a way that leaves them in an untouched condition. An alternative would be to see if you can get access to a high quality stereomicroscope - ideally one with software that allows extended depth of focus (EDF) images to be taken. One problem with a conventional stereoscope is that you can see the top of the insect in focus but not the bottom or vice versa. With EDF software, one can grab a digital image of each focal plane and the software reconstructs an image that is in good focus for the entire depth of the specimen. We have a motorized Leica MTOF FA stereomicroscope in our core facility and clients working with insects love it. You can see some examples of images taken with and without EDF on this microscope at our website http://www.biotech.missouri.edu/mcc/Stereoscope_M205.html . Good luck. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Saturday, June 28, 2014 7:35 AM To: Phillips, Thomas E.
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Title-Subject: [Filtered] SEM - Need help with which scope to use for insects
Message: I am an undergraduate student at the University of Manitoba working on a revision of a genus of parasitic wasps. I have three new species that I would like to take images of in order to see their sculpturing, and I'm trying to figure out which SEM I should use. The specimens are about 3mm in length, and because they are not mine, I need them to remain as is (they have to be returned in the same condition in which I received them). The insects are point mounted, which means they are glued to the end of a small, pointed piece of paper which then has a pin stuck through it. The glue used likely dissolves in ethanol.
The two scopes we have here at the university are: Philips XL 30 and JEOL JSM-5900LV. I'm hoping someone could provide me with a recommendation on which to use, and advise me on what I will need to do if one of these scopes is suitable.
Any information would be of great help! Thank you,
Melanie Scallion
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==============================Original Headers============================== 23, 33 -- From PhillipsT-at-missouri.edu Sat Jun 28 10:11:05 2014 23, 33 -- Received: from um-nip4-missouri-out.um.umsystem.edu (um-nip4-missouri-out.um.umsystem.edu [198.209.49.177]) 23, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5SFB4RU024430 23, 33 -- for {microscopy-at-microscopy.com} ; Sat, 28 Jun 2014 10:11:04 -0500 23, 33 -- X-IronPort-Anti-Spam-Filtered: true 23, 33 -- X-IronPort-Anti-Spam-Result: Ag0FABjarlPPoJ7U/2dsb2JhbABXA4MNUlrEWAGBCBZ1hAMBAQEEOhQ3AgICAQgRAwEBAQsCCgEHCQcWGwEUCQgCBAEJCQgVBIghDb9ehXsXBIxmEoEnEQEfDxISBQYLAQGDGoEWBYVihwiJXIFVhAiSNYNCbAGBAwcXIg 23, 33 -- X-IPAS-Result: Ag0FABjarlPPoJ7U/2dsb2JhbABXA4MNUlrEWAGBCBZ1hAMBAQEEOhQ3AgICAQgRAwEBAQsCCgEHCQcWGwEUCQgCBAEJCQgVBIghDb9ehXsXBIxmEoEnEQEfDxISBQYLAQGDGoEWBYVihwiJXIFVhAiSNYNCbAGBAwcXIg 23, 33 -- Received: from um-ncas6.um.umsystem.edu ([207.160.158.212]) 23, 33 -- by um-nip4-exch-relay.um.umsystem.edu with ESMTP; 28 Jun 2014 10:11:04 -0500 23, 33 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.161]) by 23, 33 -- UM-NCAS6.um.umsystem.edu ([207.160.158.212]) with mapi id 14.03.0181.006; 23, 33 -- Sat, 28 Jun 2014 10:11:03 -0500 23, 33 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 23, 33 -- To: "scalliom-at-myumanitoba.ca" {scalliom-at-myumanitoba.ca} , 23, 33 -- "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 23, 33 -- Subject: RE: [Microscopy] viaWWW:SEM - Need help with which scope to use for 23, 33 -- insects 23, 33 -- Thread-Topic: [Microscopy] viaWWW:SEM - Need help with which scope to use 23, 33 -- for insects 23, 33 -- Thread-Index: AQHPks1zA/PJezqNsk+15fW7mY/bLJuGnoqQ 23, 33 -- Date: Sat, 28 Jun 2014 15:11:02 +0000 23, 33 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DDBE5DD0B-at-UM-MBX-T02.um.umsystem.edu} 23, 33 -- References: {201406281235.s5SCZSrf012503-at-ns.microscopy.com} 23, 33 -- In-Reply-To: {201406281235.s5SCZSrf012503-at-ns.microscopy.com} 23, 33 -- Accept-Language: en-US 23, 33 -- Content-Language: en-US 23, 33 -- X-MS-Has-Attach: 23, 33 -- X-MS-TNEF-Correlator: 23, 33 -- x-originating-ip: [161.130.252.55] 23, 33 -- Content-Type: text/plain; charset="us-ascii" 23, 33 -- MIME-Version: 1.0 23, 33 -- Content-Transfer-Encoding: 8bit 23, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5SFB4RU024430 ==============================End of - Headers==============================
Hi Melanie, Tom's suggestion to use EDF is a good one. Those motorized Leica systems are excellent and pricey. Good manual alternatives are available at much lower cost. A good DSLR kit on a stereo with Helicon focus or other free or inexpensive software packages do a good job. Just ensure the software has a stereo microscope plugin to account for the lateral image shift as the stereo is focused. Jim
-- JIM SCHULTE
Micro Source Imaging 612 770-3885 www.ms-imaging.com Sales and service of Canon, Motic and Diagnostic Instruments cameras Motic, Accu-scope and Unitron microscopes
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FYI a summary of responses. Thanks much to all, very useful.
1. Alternatives are available, but expensive. These include: - firewalls around each room lacking sprinklers, and firedoor entryway - rather expensive. The walls and door have to be able to resist fire for 1 hour. - gas systems - also expensive, and in Australia these are apparently only allowed in non-occupied rooms (I am told, not going to fight it, save fight for other stuff...).
So
2. Accept the inevitability of sprinklers, and insist on the following: - sprinkler head(s) as far from instrument as possible, esp. from main power supplies - sprinkler heads enclosed in wire cages to prevent accidental knocking when moving large items in the room
3. Realise that floods from pipes/sinks/rain events are much more likely than sprinkler activation, so: - install drip pans above the sprinklers, either below the ceiling or within it, to deflect potential floods from above away from the instrument - raise important components at least several cm above floor level - have a system that only fills the sprinkler pipes when the sprinkler(s) are activated to avoid slow leaks (drips)
4. Also realise that sprinkler pipes may be sources of conductive interference, so: - ask that pipes are empty unless sprinkler(s) activated - insert a rubber or other non-metallic connection between the main sprinkler supply system and the sprinklers in instrument rooms
and also, check your insurance policy!
Like others, the only floods I've experienced were from other sources - in a previous institution on one torrential weekend several staff came in to check for leaks and ended up doing a bucket brigade from the EM unit in the basement. All fine, except for the piles of paperwork around desks...
And Roger Ristau's email reminded me that about 15 years ago the in lens cooling system in our JEOL 6400 developed a leak and flooded the lens, detected by water dripping down into the chamber - messy! The old girl is still chugging along, though not for much longer - the old electronic boards are cracking and won't survive the move, so turning off for good in October. :(
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry (for 1 more day) GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
==============================Original Headers============================== 14, 45 -- From prvs=250a004e1=Rosemary.White-at-csiro.au Sun Jun 29 02:51:09 2014 14, 45 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au [150.229.7.43]) 14, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5T7p7iC010061 14, 45 -- for {microscopy-at-microscopy.com} ; Sun, 29 Jun 2014 02:51:08 -0500 14, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 14, 45 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; 14, 45 -- t=1404028269; x=1435564269; 14, 45 -- h=from:to:subject:date:message-id:content-id: 14, 45 -- content-transfer-encoding:mime-version; 14, 45 -- bh=d5QG/++PVxyaeUHV7sI+hoMktaCaBZIgQSPfLKbFGdM=; 14, 45 -- b=iFZmsqfe/E/9zJYDLn/dJsfKwOn9Rao6BxiNg8X4ezueZ6IRazKDa2uD 14, 45 -- 5KOlebK1kF/pya68uBg3+XFG2DDLy1g1CYGCazaYFcp2PJ51u1cilyd+p 14, 45 -- g/sECfuoTfynfex; 14, 45 -- X-SBRS: 4.0 14, 45 -- X-IronPort-Anti-Spam-Filtered: true 14, 45 -- X-IronPort-Anti-Spam-Result: A+CJDQD8xK9TjACwBSSwhIATAJKcgCJZDoJbgVDDdIJdFg8BAQEnPIQKOlEBPkInBAESiEIBxgYXjiURAQKFGAWaXotqixNCgXc5 14, 45 -- X-IPAS-Result: A+CJDQD8xK9TjACwBSSwhIATAJKcgCJZDoJbgVDDdIJdFg8BAQEnPIQKOlEBPkInBAESiEIBxgYXjiURAQKFGAWaXotqixNCgXc5 14, 45 -- X-IronPort-AV: E=Sophos;i="5.01,569,1399989600"; 14, 45 -- d="scan'208";a="125426033" 14, 45 -- Received: from exhtca02-cdc.nexus.csiro.au ([IPv6:2405:b000:601:13::247:22]) 14, 45 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES128-SHA; 29 Jun 2014 17:50:41 +1000 14, 45 -- Received: from EXHTCA01-CDC.nexus.csiro.au (2405:b000:601:13::247:21) by 14, 45 -- ExHTCA02-CDC.nexus.csiro.au (2405:b000:601:13::247:22) with Microsoft SMTP 14, 45 -- Server (TLS) id 14.3.158.1; Sun, 29 Jun 2014 17:50:41 +1000 14, 45 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80::9de3:d7af:4ed1:b7b6]) by 14, 45 -- exhtca01-cdc.nexus.csiro.au ([fe80::296a:b134:b85b:89de%13]) with mapi id 14, 45 -- 14.03.0158.001; Sun, 29 Jun 2014 17:50:41 +1000 14, 45 -- From: {Rosemary.White-at-csiro.au} 14, 45 -- To: {microscopy-at-microscopy.com} , {CONFOCALMICROSCOPY-at-LISTS.UMN.EDU} 14, 45 -- Subject: summary: fire sprinklers in instrument rooms 14, 45 -- Thread-Topic: summary: fire sprinklers in instrument rooms 14, 45 -- Thread-Index: AQHPk27SZCVQReZWWUmZdwpAeqR3Kw== 14, 45 -- Date: Sun, 29 Jun 2014 07:50:40 +0000 14, 45 -- Message-ID: {CFD60271.164DEE%rosemary.white-at-csiro.au} 14, 45 -- Accept-Language: en-NZ, en-US, en-AU 14, 45 -- Content-Language: en-US 14, 45 -- X-MS-Has-Attach: 14, 45 -- X-MS-TNEF-Correlator: 14, 45 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 14, 45 -- x-originating-ip: [152.83.195.117] 14, 45 -- Content-Type: text/plain; charset="us-ascii" 14, 45 -- Content-ID: {F9A9F90F3359E74190A405AA8436B5BE-at-csiro.au} 14, 45 -- MIME-Version: 1.0 14, 45 -- Content-Transfer-Encoding: 8bit 14, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5T7p7iC010061 ==============================End of - Headers==============================
I assume not too many rooms have been flooded from accidental releases from sparkler systems, but I am aware of some accidental releases from sprinkler systems.
I had a project come through years ago where a consulting engineer was looking at the metal fuse in a sprinkler element. It had released a flooded a store. His question was whether its release was prompted by a thermal event or a mechanical event. I suppose both would show some signs of ductile rupture and he did not have an exemplar for us to examine from the two possibilities.
On the flood story side, we are waiting for the plumbers to resolved a problem in the bathroom upstairs. Someone saw fit to replumb with low-flow valves. However, the replumbing has failed on at least two occasions leading to lots of "clean" water coming through the floor into our lab below. I don't know what the savings in water would have been but the initial costs of replumbing, but the costs of clean-up and the necessary replacement of ceiling tiles has certainly lengthened out the pay-back period. I am really leery of "upgrades".
Warren
-----Original Message----- X-from: jfmjfm-at-umich.edu [mailto:jfmjfm-at-umich.edu] Sent: Friday, June 27, 2014 2:47 PM To: Straszheim, Warren E [BIOTC]
I just love the Apple autocorrect! Sprinkler systems. I am willing to bet that NO-ONE has experienced an accidental release of water from a sprinkler system. Flooding from other labs or burst pipes, yes, but sprinklers I think not.
____ John Mansfield PhD Cphys MInstP *****Note New Business Mailing Address, all other information remains the same***** Microscopy Society of America - President-Elect Associate Director North Campus Electron Microbeam Analysis Laboratory Building 22, Room G010, University of Michigan 2800 Plymouth Road, Ann Arbor MI 48109-2800 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/ iMessage: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096
On Jun 27, 2014, at 12:26 PM, Philip Oshel {oshel1pe-at-cmich.edu} wrote:
} John, } } A very relevant question, given the 4th of July is next week, but I don't think many people have "sparkler systems" in their microscopy labs. } Would be pretty and exciting, though ... } } Phil } } } } My question is is: } } "How many people have experienced sparkler systems go off in their microscope rooms?" } } Anyone want to jump in a relate experiences? } } } } } } ____ } } John Mansfield PhD Cphys MInstP } } *****Note New Business Mailing Address, all other information remains the same***** } } Microscopy Society of America - President-Elect } } Associate Director } } North Campus Electron Microbeam Analysis Laboratory } } Building 22, Room G010, University of Michigan } } 2800 Plymouth Road, Ann Arbor MI 48109-2800 USA } } Phone: (734) 936-3352 FAX (734) 763-2282 } } Cell. Phone: (734) 834-3913 } } Email: jfmjfm-at-umich.edu } } URL: http://emalwww.engin.umich.edu/ } } iMessage: thejfmjfm } } Skype: thejfmjfm } } } } Home address: } } 4304 Spring Lake Boulevard } } Ann Arbor MI 48108-9657 } } Phone (734) 994-3096 } } } } On Jun 27, 2014, at 9:17 AM, barrylamb-at-meadtest.com wrote: } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Hi Rosemary, } } } } } } We had Perspex canopies made up for our SEMs, Auger system and SIMS } } } machines to protect from direct sprinkler spray. } } } } } } Regards, Barry } } } } } } Barry Lamb } } } Director } } } Mead Testing Ltd } } } Registered address: 25 Mead Park,River Way, Harlow, Essex, CM20 2SE, UK } } } Company no. 4572600 } } } barrylamb-at-meadtest.com } } } www.meadtest.com } } } tel: + 44 (0) 1279 635864 } } } fax: +44 (0) 1279 635874 } } } } } } } } } -----Original Message----- } } } X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] } } } Sent: 27 June 2014 13:21 } } } To: barrylamb-at-meadtest.com } } } Subject: [Microscopy] RE: Sprinklers in instrument rooms } } } } } } } } } } } } } } } ------------------------------------------------------------------------ } } } ---- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } } } ---- } } } } } } Hi Rosemary } } } } } } Out of interest I have dealt with water cascading over microscopes, yes } } } it } } } has happened twice in my 49 electron microscopy years. As a service } } } technician the main problem is not so much the water, but the debris } } } that } } } the water beings down onto the electrical circuits. Both of the } } } instruments } } } in question were running on vacuum only and managed to keep going } } } overnight } } } until security found the water running through the particular } } } laboratories. } } } One was a tap exploding, shooting a fountain of water into the EM room, } } } the } } } other the result of putting out a fire three levels above. } } } } } } A third situation occurred when a water pipe came off one instrument in } } } a } } } basement EM unit, flooding the complete area to about 6 inches. Everyone } } } of } } } the instruments kept going, but as I arrived a technician with rubber } } } boots } } } and a long wooden pole was taking care of switching the most endangered } } } instruments off. However this was in the 1980s when most EM Units were } } } underground; the one I mentioned was also used as a Tornado shelter! } } } } } } EM Units and water, great fun! } } } } } } Steve } } } } } } Steve Chapman FRMS } } } Protrain for Consultancy and Courses in Electron Microscopy } } } Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 } } } www.emcourses.com } } } } } } -----Original Message----- } } } X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] } } } Sent: 26 June 2014 11:09 } } } To: protrain-at-emcourses.com } } } Subject: [Microscopy] Sprinklers in instrument rooms } } } } } } Hi all, } } } } } } I know this has been discussed before, just wondered if there were any } } } new } } } thoughts. We're moving twice, first into a refurbished basement, second } } } time } } } into a new building. The basement has a sprinkler system in case of } } } fire, } } } unlike our current building which just has alarms (it's a single storey } } } brick building). I imagine the new building will have some sort of } } } sprinkler } } } system too. } } } } } } What precautions, if any, do people take to protect against the unlikely } } } showering of your confocal or EM? } } } Are there any relatively simple alternatives? (One alternative is to } } } have } } } fire-doors and walls for each room but that is prohibitive.) } } } } } } thanks much, } } } Rosemary } } } } } } Dr Rosemary White } } } CSIRO Plant Industry (for 4 more days only) GPO Box 1600 Canberra, ACT } } } 2601 } } } } } } T 02 6246 5475 } } } F 02 6246 5334 } } } E rosemary.white-at-csiro.au } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } 8, 42 -- From prvs=247c2aa8e=Rosemary.White-at-csiro.au Thu Jun 26 05:06:26 } } } 2014 8, 42 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au } } } [150.229.7.43]) } } } 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id } } } s5QA6PA5015134 } } } 8, 42 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2014 } } } 05:06:26 } } } -0500 } } } 8, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } } } 8, 42 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; } } } 8, 42 -- t=1403777186; x=1435313186; 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Pleasant, MI 48859 } (989) 774-3576 }
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==============================Original Headers============================== 17, 32 -- From wesaia-at-iastate.edu Sun Jun 29 12:23:03 2014 17, 32 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2lp0237.outbound.protection.outlook.com [207.46.163.237]) 17, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5THN2In019357 17, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 29 Jun 2014 12:23:02 -0500 17, 32 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 17, 32 -- BLUPR04MB545.namprd04.prod.outlook.com (10.141.29.139) with Microsoft SMTP 17, 32 -- Server (TLS) id 15.0.974.11; Sun, 29 Jun 2014 17:22:58 +0000 17, 32 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.59]) by 17, 32 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.59]) with mapi id 17, 32 -- 15.00.0969.007; Sun, 29 Jun 2014 17:22:58 +0000 17, 32 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 17, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 17, 32 -- Subject: RE: [Microscopy] Sprinklers in instrument rooms 17, 32 -- Thread-Topic: [Microscopy] Sprinklers in instrument rooms 17, 32 -- Thread-Index: AQHPkkC67SzK1okdTE+9B1wyGMbVf5uIVrVg 17, 32 -- Date: Sun, 29 Jun 2014 17:22:57 +0000 17, 32 -- Message-ID: {0903115d7a0d424198e530c55fffcb8e-at-BLUPR04MB546.namprd04.prod.outlook.com} 17, 32 -- References: {201406271947.s5RJlTSU015874-at-ns.microscopy.com} 17, 32 -- In-Reply-To: {201406271947.s5RJlTSU015874-at-ns.microscopy.com} 17, 32 -- Accept-Language: en-US 17, 32 -- Content-Language: en-US 17, 32 -- X-MS-Has-Attach: 17, 32 -- X-MS-TNEF-Correlator: 17, 32 -- x-originating-ip: [69.66.102.132] 17, 32 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID: 17, 32 -- x-forefront-prvs: 025796F161 17, 32 -- x-forefront-antispam-report: SFV:NSPM;SFS:(6009001)(24454002)(13464003)(252514010)(199002)(189002)(377454003)(41584004)(53754006)(48214007)(51704005)(64706001)(21056001)(106356001)(575784001)(86362001)(92566001)(80022001)(76482001)(76176999)(50986999)(19580395003)(20776003)(19580405001)(83322001)(15975445006)(105586002)(106116001)(33646001)(74316001)(46102001)(79102001)(4396001)(87936001)(99396002)(15202345003)(75432001)(2656002)(77982001)(54356999)(512954002)(99286002)(85852003)(85306003)(15974865002)(83072002)(77096002)(101416001)(95666004)(2351001)(107886001)(107046002)(81542001)(66066001)(81342001)(88552001)(74502001)(31966008)(74662001)(76576001)(24736002)(579004)(19627235001);DIR:OUT;SFP:;SCL:1;SRVR:BLUPR04MB545;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;MX:1;LANG:en; 17, 32 -- Content-Type: text/plain; charset="us-ascii" 17, 32 -- MIME-Version: 1.0 17, 32 -- X-OriginatorOrg: iastate.edu 17, 32 -- Content-Transfer-Encoding: 8bit 17, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5THN2In019357 ==============================End of - Headers==============================
I agree that the light microscope and EDF software would produce some great images. I have produced some fun images of bees, flies, and mosquitos with a stereo microscope and free edf software called Combined ZM. The natural color of the light microscope images adds to the image compared to gray-scale SEM images for low-mag work.
For SEM, the JEOL 5900LV will be your best bet for examination in an undisturbed state. In the LV mode, you can observe the wasps without coating and should be able to get some good images of general physical characteristics. You will not be able to get real high magnification images since you will be limited to backscattered imaging You would have to sputter coat the samples to image secondary electrons in the 5900LV or the Philips XL 30 for higher magnifications. If you can sputter coat a specimen, you should be able to get great images of the fine details with the Philips.
Good luck.
On 6/28/2014 7:35 AM, microscopylistserver-noreply-at-microscopy.com wrote: } Email:scalliom-at-myumanitoba.ca } Name: Melanie Scallion } } Organization: University of Manitoba } } Title-Subject: [Filtered] SEM - Need help with which scope to use for insects } } Message: I am an undergraduate student at the University of Manitoba working on a revision of a } genus of parasitic wasps. I have three new species that I would like to take images of in order to } see their sculpturing, and I’m trying to figure out which SEM I should use. The specimens are about } 3mm in length, and because they are not mine, I need them to remain as is (they have to be returned } in the same condition in which I received them). The insects are point mounted, which means they are } glued to the end of a small, pointed piece of paper which then has a pin stuck through it. The glue } used likely dissolves in ethanol. } } The two scopes we have here at the university are: Philips XL 30 and JEOL JSM-5900LV. I’m hoping } someone could provide me with a recommendation on which to use, and advise me on what I will need to } do if one of these scopes is suitable. } } Any information would be of great help! Thank you, } } Melanie Scallion
-- ________ Larry D. Hanke, P.E. Principal Engineer Materials Evaluation and Engineering, Inc. (763) 449-8870
==============================Original Headers============================== 8, 19 -- From hanke-at-mee-inc.com Sun Jun 29 12:41:16 2014 8, 19 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5THfGDp028316 8, 19 -- for {microscopy-at-microscopy.com} ; Sun, 29 Jun 2014 12:41:16 -0500 8, 19 -- Received: (qmail 10736 invoked by uid 507); 29 Jun 2014 12:41:15 -0500 8, 19 -- Received: from c-24-118-160-10.hsd1.mn.comcast.net (HELO ?192.168.1.73?) (24.118.160.10) 8, 19 -- by mail.namisolutions.com with SMTP; 29 Jun 2014 12:41:15 -0500 8, 19 -- Message-ID: {53B04FBB.7070509-at-mee-inc.com} 8, 19 -- Date: Sun, 29 Jun 2014 12:41:15 -0500 8, 19 -- From: Larry Hanke {hanke-at-mee-inc.com} 8, 19 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 8, 19 -- MIME-Version: 1.0 8, 19 -- To: microscopy-at-microscopy.com, scalliom-at-myumanitoba.ca 8, 19 -- Subject: Re: [Microscopy] viaWWW:SEM - Need help with which scope to use for 8, 19 -- insects 8, 19 -- References: {201406281235.s5SCZO07012317-at-ns.microscopy.com} 8, 19 -- In-Reply-To: {201406281235.s5SCZO07012317-at-ns.microscopy.com} 8, 19 -- Content-Type: text/plain; charset=windows-1252; format=flowed 8, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From drugstore_discounted6-at-expertiserealty.com Sun Jun 29 20:13:39 2014 Return-Path: {drugstore_discounted6-at-expertiserealty.com} Received: from mail3.expertiserealty.com ([62.176.8.138]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5U1DMHk028210 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 29 Jun 2014 20:13:22 -0500 Message-Id: {201406300113.s5U1DMHk028210-at-ns.microscopy.com}
Hi all,
As mentioned, we're eventually moving into a new building, and if possible, we'll incorporate all the best building recommendations. I remember when all TEM and SEM rooms had floating floors to eliminate or at least substantially reduce vibrational noise. Is this still the best option? It's rather expensive and if not really needed we could divert the saved funds elsewhere. We are planning to have a plant room in which all the noisy components - pumps and fans, mainly, are isolated from the main instruments.
We have a VP-SEM and have a FESEM on the to-buy list for 2-3 years' time. No TEM any more. We'll be on the ground floor and it's a fairly vibration-free site - no main roads nearby, etc. Just need to make sure the threshing and grinding equipment isn't installed next door...
thanks much, Rosemary
Dr Rosemary White CSIRO Plant Industry (last day) GPO Box 1600 Canberra, ACT 2601
T 02 6246 5475 F 02 6246 5334 E rosemary.white-at-csiro.au
I would like to inform that the same (micro-leak in the cooling line at the junction plastic hose to copper-fitting of the projective lens of my ZEISS EM109) happened in our EM-Lab (it was 18th April 2000 after nearly 20 years of operation).
It took some hours to get rid of all the water within the viewing chamber and the 6x9 camera beneath the viewing chamber.
Needed a new projective lens, which is in function until now... Not remembering what the Cost for repair were. So, I think it will be time to either have an eye on it or to put it into mothballs.....(;-()
Have a great and accident-free week, regards, Wolfgang
Wolfgang Muss SALZBURG-AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: microscopylistserver-noreply-at-microscopy.com } [mailto:microscopylistserver-noreply-at-microscopy.com] } Gesendet: Samstag, 28. Juni 2014 14:42 } An: Muß Wolfgang } Betreff: [Microscopy] Re: Sprinklers in instrument rooms } } ----------------------------------------------------------------------- } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } X-from: roger.ristau-at-uconn.edu () } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both roger.ristau-at-uconn.edu as well as the Microscopy } Listserver } ----------------------------------------------------------------------- } ---- } Email: roger.ristau-at-uconn.edu Name: Roger Ristau } Organization: University of Connecticut } Title-Subject: Re: Sprinklers in instrument } rooms } Message: } } Here is a situation that I bet not many have encountered: } We had a TEM which developed a leak in lens cooling with the result } that the INSIDE of the TEM filled with water. (I was not here } but was told the camera/viewing chamber looked like "a fish bowl.") } } My predecessor, Larry McCurdy, courageously attacked the mess and } restored the TEM to service! It soldiered on for many years. } Point of story: Flooding the OUTSIDE of the TEM may actually be more } harmful (electrical shorts,etc.) than flooding the INSIDE! } } Cheers } Roger } } Institute of Materials Science } University of Connecticut } } } Login Host: 137.99.20.190 } Listserver Email Form V - 20120416 } ----------------------------------------------------------------------- } ---- } } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original } Headers============================== } 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Jun 28 } 07:30:40 2014 } 17, 28 -- Received: from znl.com ([206.69.208.20]) } 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s5SCUcqo010062 } 17, 28 -- for {microscopy-at-microscopy.com} ; Sat, 28 Jun 2014 07:30:40 } -0500 } 17, 28 -- Received: from localhost (localhost [127.0.0.1]) } 17, 28 -- by znl.com (Postfix) with ESMTP id CCE7E721F02 } 17, 28 -- for {microscopy-at-microscopy.com} ; Sat, 28 Jun 2014 07:30:38 } -0500 (CDT) } 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 17, 28 -- Received: from znl.com ([127.0.0.1]) } 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd- } new, port 10024) } 17, 28 -- with ESMTP id 7QuQ3zuaPTEQ for {microscopy-at-microscopy.com} ; } 17, 28 -- Sat, 28 Jun 2014 07:30:33 -0500 (CDT) } 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com } [206.69.208.22]) } 17, 28 -- by znl.com (Postfix) with ESMTPA id 0E29B721EF5 } 17, 28 -- for {microscopy-at-microscopy.com} ; Sat, 28 Jun 2014 07:30:33 } -0500 (CDT) } 17, 28 -- Message-ID: {53AEB568.1040602-at-microscopy.com} } 17, 28 -- Date: Sat, 28 Jun 2014 07:30:32 -0500 } 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver- } noreply-at-microscopy.com} } 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; } rv:24.0) Gecko/20100101 Thunderbird/24.6.0 } 17, 28 -- MIME-Version: 1.0 } 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 17, 28 -- Subject: viaWWW:Sprinklers in instrument rooms } 17, 28 -- References: {201406272018.s5RKIUQ6021053-at-ns.microscopy.com} } 17, 28 -- In-Reply-To: {201406272018.s5RKIUQ6021053-at-ns.microscopy.com} } 17, 28 -- X-Forwarded-Message-Id: } {201406272018.s5RKIUQ6021053-at-ns.microscopy.com} } 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 17, 28 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 38 -- From W.Muss-at-salk.at Mon Jun 30 02:56:47 2014 10, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 10, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5U7ukvm017887 10, 38 -- for {microscopy-at-microscopy.com} ; Mon, 30 Jun 2014 02:56:47 -0500 10, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 10, 38 -- by hermes.salk.at (Postfix) with ESMTP id 9D60A646B2E 10, 38 -- for {microscopy-at-microscopy.com} ; Mon, 30 Jun 2014 09:56:45 +0200 (CEST) 10, 38 -- X-Scanned-By: SALK-Content-Filter 10, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 10, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 10, 38 -- with ESMTP id FfSFHzSr_E_b for {microscopy-at-microscopy.com} ; 10, 38 -- Mon, 30 Jun 2014 09:56:45 +0200 (CEST) 10, 38 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 10, 38 -- by hermes.salk.at (Postfix) with ESMTP id 78238646B2D 10, 38 -- for {microscopy-at-microscopy.com} ; Mon, 30 Jun 2014 09:56:45 +0200 (CEST) 10, 38 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 10, 38 -- by N1EX198.lks.local with ESMTP; 30 Jun 2014 10:06:25 +0200 10, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 10, 38 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 10, 38 -- 14.02.0387.000; Mon, 30 Jun 2014 09:56:45 +0200 10, 38 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 10, 38 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 10, 38 -- Subject: [Microscopy] Re: Sprinklers in instrument rooms 10, 38 -- Thread-Topic: [Microscopy] Re: Sprinklers in instrument rooms 10, 38 -- Thread-Index: AQHPlDddB59MEk6gP06D7BQPDl057A== 10, 38 -- Date: Mon, 30 Jun 2014 07:56:44 +0000 10, 38 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D3A94F7-at-N2DT131.lksdom21.lks.local} 10, 38 -- References: {201406281242.s5SCgIOv026749-at-ns.microscopy.com} 10, 38 -- In-Reply-To: {201406281242.s5SCgIOv026749-at-ns.microscopy.com} 10, 38 -- Accept-Language: de-DE, en-US 10, 38 -- Content-Language: de-DE 10, 38 -- X-MS-Has-Attach: 10, 38 -- X-MS-TNEF-Correlator: 10, 38 -- x-originating-ip: [192.168.42.2] 10, 38 -- Content-Type: text/plain; charset="iso-8859-1" 10, 38 -- MIME-Version: 1.0 10, 38 -- Content-Transfer-Encoding: 8bit 10, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5U7ukvm017887 ==============================End of - Headers==============================
We moved the department wholesale into a new building last August. During the design stage I was heavily involved in benchmarking good laboratories with the structural engineers (Ramboll, www.ranboll.co.uk) and our vibration/noise consultant, Michael Gendreau of Colin Gordons & Associates (www.colingordon.com).
Michael was instrumental in our decision to do without isolation plinths when he demonstrated that, in the low frequency regime, i.e. below the resonant frequency of the plinth, the amplitude of the vibration was worse than the outside structure, i.e. it was an amplifier of noise. Above the resonant frequency, the plinth attenuated it, as expected (referring to a damped harmonic oscillator). The Q-factor of each plinth was determined by the damping material underneath the plinth. In some cases the isolation block was moving 100 times faster (at resonance) than the surrounding building (in one lab we tested).
The money was spent on making the single raft slab thicker - we doubled it from the early design thickness of 1 metre to 2 metres thick and that is what we have now (the main department building is 0.5m thick). Further, we have separate room-within-a-room build with each microscope having a cross-laminate timber walls and ceiling that are 20cm thick. The rooms were finsihed with acoustic panels on the ceiling and 2 walls to reduce noise. Room cooling was with a chilled ceiling (95% cooling) with two plenums for a little cool fresh air. For a view of the room, see here:
We have found that this works really well for us. For example, we were getting information out to 0.9 Angstrom with an FEI Titan TEM in the old building, but now, in the new, heavy, foundation we get information down to 0.6 to 0.7 Angstrom.
In summary, the foundation of the building is as big and heavy as we could make it and we avoided floating tables/isolation plinths. Michael acknowledged that this was what they were recommending ten-to-fifteen years ago, but have decided against it since when evidence accumulated that they only work in certain circumstances.
I hope this helps.
Jon
} Hi all, } } As mentioned, we're eventually moving into a new building, and if } possible, we'll incorporate all the best building recommendations. I } remember when all TEM and SEM rooms had floating floors to eliminate or } at } least substantially reduce vibrational noise. Is this still the best } option? It's rather expensive and if not really needed we could divert } the } saved funds elsewhere. We are planning to have a plant room in which } all } the noisy components - pumps and fans, mainly, are isolated from the } main } instruments. } } We have a VP-SEM and have a FESEM on the to-buy list for 2-3 years' } time. } No TEM any more. We'll be on the ground floor and it's a fairly } vibration-free site - no main roads nearby, etc. Just need to make sure } the threshing and grinding equipment isn't installed next door... } } thanks much, } Rosemary } } Dr Rosemary White } CSIRO Plant Industry (last day) } GPO Box 1600 } Canberra, ACT 2601 } } T 02 6246 5475 } F 02 6246 5334 } E rosemary.white-at-csiro.au
==============================Original Headers============================== 11, 31 -- From jsb43-at-cam.ac.uk Mon Jun 30 06:49:31 2014 11, 31 -- Received: from ppsw-52.csi.cam.ac.uk (ppsw-52.csi.cam.ac.uk [131.111.8.152]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5UBnUV7006661 11, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jun 2014 06:49:30 -0500 11, 31 -- X-Cam-AntiVirus: no malware found 11, 31 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 11, 31 -- Received: from webmail-2a.csi.cam.ac.uk ([131.111.9.34]:40055) 11, 31 -- by ppsw-52.csi.cam.ac.uk (ppsw.cam.ac.uk [131.111.8.139]:25) 11, 31 -- with esmtp id 1X1a5Q-0002or-D2 (Exim 4.82_3-c0e5623) 11, 31 -- (return-path {jsb43-at-cam.ac.uk} ); Mon, 30 Jun 2014 12:49:28 +0100 11, 31 -- Received: from [127.0.0.1] (helo=webmail-2.hermes.cam.ac.uk) 11, 31 -- by webmail-2a.csi.cam.ac.uk with esmtp id 1X1a5P-0004Ej-Tj (Exim 4.80_167-5a66dd3) 11, 31 -- (return-path {jsb43-at-cam.ac.uk} ); Mon, 30 Jun 2014 12:49:27 +0100 11, 31 -- Received: from sunset.msm.cam.ac.uk ([131.111.102.36]) 11, 31 -- by webmail-2.hermes.cam.ac.uk 11, 31 -- with HTTP (HTTP/1.1 POST); Mon, 30 Jun 2014 12:49:27 +0100 11, 31 -- MIME-Version: 1.0 11, 31 -- Content-Type: text/plain; charset=UTF-8; 11, 31 -- format=flowed 11, 31 -- Content-Transfer-Encoding: 7bit 11, 31 -- X-Authenticated-User: jsb43 11, 31 -- Date: Mon, 30 Jun 2014 12:49:27 +0100 11, 31 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 11, 31 -- To: Rosemary.White-at-csiro.au 11, 31 -- Cc: Listserver MSA {Microscopy-at-microscopy.com} 11, 31 -- Subject: Re: floating floors for SEMs 11, 31 -- In-Reply-To: {201406300630.s5U6UbZ2006479-at-ns.microscopy.com} 11, 31 -- References: {201406300630.s5U6UbZ2006479-at-ns.microscopy.com} 11, 31 -- Message-ID: {7b1a3a230bebd1d2df541210daa3169c-at-cam.ac.uk} 11, 31 -- X-Sender: jsb43-at-cam.ac.uk 11, 31 -- User-Agent: Roundcube Webmail/0.9.4 ==============================End of - Headers==============================
There are many reasons why a tip may become unstable but one of the most common is a build up of material inside the cathode aperture.
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] beam fluctuation in FEI T20
Message: Hello,
Variation in beam intensity is observed with LaB6. How ever the LaB6 filament is showing the connectivity. What could be the possible problem.
Rashmi
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==============================Original Headers============================== 20, 24 -- From protrain-at-emcourses.com Mon Jun 30 09:26:05 2014 20, 24 -- Received: from mail.esentra.net (mail.esentra.net [94.228.36.208]) 20, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5UEQ4jO027957 20, 24 -- for {microscopy-at-microscopy.com} ; Mon, 30 Jun 2014 09:26:05 -0500 20, 24 -- Received: from mail.clientmail.net (Not Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway 20, 24 -- id {B53b173750000} ; Mon, 30 Jun 2014 15:25:57 +0100 20, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Mon, 30 Jun 2014 15:25:57 +0100 20, 24 -- Reply-To: {protrain-at-emcourses.com} 20, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} 20, 24 -- To: {rashmi_mehata-at-yahoo.com} 20, 24 -- Cc: "'Microscopical Soc of America'" {microscopy-at-microscopy.com} 20, 24 -- References: {201406281236.s5SCa4sD014057-at-ns.microscopy.com} 20, 24 -- In-Reply-To: {201406281236.s5SCa4sD014057-at-ns.microscopy.com} 20, 24 -- Subject: RE: [Microscopy] Fwd: viaWWW:beam fluctuation in FEI T20 20, 24 -- Date: Mon, 30 Jun 2014 15:26:05 +0100 20, 24 -- Organization: Protrain 20, 24 -- Message-ID: {000901cf946f$3a44c5a0$aece50e0$-at-com} 20, 24 -- MIME-Version: 1.0 20, 24 -- Content-Type: text/plain; 20, 24 -- charset="us-ascii" 20, 24 -- Content-Transfer-Encoding: 7bit 20, 24 -- X-Mailer: Microsoft Office Outlook 12.0 20, 24 -- Thread-Index: Ac+SzYaHbVqiFaPkRJCILxiMYs2pFgBoSruQ 20, 24 -- Content-Language: en-gb ==============================End of - Headers==============================
I would recommend that you contact Sam Droeg. He works for the U. S. Geological Survey Department, and has developed a technique and hardware system for macrophotography of insects. He has posted remarkable insect photos on the web, and can talk you through your macrophotography need with a digital camera and macro lens. His contact information is: sdroege-at-usgs.gov 301 497 5840
http://www.flickr.com/photos/usgsbiml/sets/72157630468783226/ Macrophotographs of insects.
Specimens taken in Hand Sanitizer:
http://www.flickr.com/photos/usgsbiml/sets/72157631861396270/ Macrophotographs of specimens taken in cuvettes filled with hand sanitizer.
Perhaps the best technical forum on macrophotography:
http://www.photomacrography.net/ Online blog of photographers who do macrophotography.
Youtube on basic USGSBIML set up:
http://www.youtube.com/watch?v=S-_yvIsucOY Video on setting up a camera for macrophotography.
USGSBIML Photoshopping technique: Note that he now has added using the color burn brush at 20% opacity to clean up the halos that bleed into the black background from "hot" color sections of the picture.
http://www.youtube.com/watch?v=Bdmx_8zqvN4 Video of how to use Adobe Bridge and Photoshop to clean up photos after image stacking.
PDF of basic USGSBIML photography set up:
ftp://ftpext.usgs.gov/pub/er/md/laurel/Droege/How%20to%20Take%20MacroPhotographs%20of%20Insects%20BIML%20Lab2.pdf PDF file with description of camera and lens setup for macrophotography.
Google Hangout demonstration of techniques https://plus.google.com/events/c5569losvskrv2nu606ltof8odo or http://www.youtube.com/watch?v=4c15neFttoU 47 minute interview showing the entire procedure of photography, with camera setup.
He uses a 3-D stitching software program called Zerene Stacker (although you could use Photoshop or other programs that handle 3-D images, if you are familiar with the software):
Zerene Stacker has several advantages over other commonly used stacking programs: • Highest quality output images, especially in difficult cases o Accurate and robust alignment and interpolation o Advanced stacking algorithms  Clean handling of hairs and bristles – no halos or contour lines  Preserves low contrast detail and avoids “stacking mush” with deep stacks o Fast and flexible retouching makes it easy to combine the best features of multiple algorithms as well as original frames • Retouching supported by all versions, even at the lowest price • Supports 8- and 16-bit input and output files • Can generate stereo and 3-D rocking animations from a single stack, even for difficult subjects with structural overlaps and bristles. • Fully utilizes modern multi-core processors and multi-processor computers • Runs on all major computer systems: Microsoft Windows®, Macintosh OS X®, Linux®.
Zerene Stacker license, as of 12/2013 • Professional Edition, $289 USD • Prosumer Edition, $189 USD • Personal Edition, $89 USD • Student Edition, $39 USD
Ed
Edward Haller, Lab Manager University of South Florida Department of Integrative Biology Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 (813)974-2676 ehaller-at-usf.edu Office: ISA 1046 http://biology.usf.edu/ib/research/facilities/
________________________________________
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Title-Subject: [Filtered] SEM - Need help with which scope to use for insects
Message: I am an undergraduate student at the University of Manitoba working on a revision of a genus of parasitic wasps. I have three new species that I would like to take images of in order to see their sculpturing, and I’m trying to figure out which SEM I should use. The specimens are about 3mm in length, and because they are not mine, I need them to remain as is (they have to be returned in the same condition in which I received them). The insects are point mounted, which means they are glued to the end of a small, pointed piece of paper which then has a pin stuck through it. The glue used likely dissolves in ethanol.
The two scopes we have here at the university are: Philips XL 30 and JEOL JSM-5900LV. I’m hoping someone could provide me with a recommendation on which to use, and advise me on what I will need to do if one of these scopes is suitable.
Any information would be of great help! Thank you,
Melanie Scallion
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==============================Original Headers============================== 38, 44 -- From ehaller-at-health.usf.edu Mon Jun 30 10:25:34 2014 38, 44 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 38, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5UFPYck015492 38, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jun 2014 10:25:34 -0500 38, 44 -- X-ASG-Debug-ID: 1404141932-045fa86a5f1bdee0001-1DjkGe 38, 44 -- Received: from EXCH02.hscnet.hsc.usf.edu ([10.119.4.42]) by hscantispam.health.usf.edu with ESMTP id dafqOvtIdrkduo33; Mon, 30 Jun 2014 11:25:32 -0400 (EDT) 38, 44 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 38, 44 -- Received: from EXCH04.hscnet.hsc.usf.edu ([169.254.2.240]) by 38, 44 -- EXCH02.hscnet.hsc.usf.edu ([169.254.4.89]) with mapi id 14.03.0181.006; Mon, 38, 44 -- 30 Jun 2014 11:25:32 -0400 38, 44 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 38, 44 -- To: "scalliom-at-myumanitoba.ca" {scalliom-at-myumanitoba.ca} , 38, 44 -- "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 38, 44 -- Subject: RE: [Microscopy] viaWWW:SEM - Need help with which scope to use for 38, 44 -- insects 38, 44 -- Thread-Topic: [Microscopy] viaWWW:SEM - Need help with which scope to use 38, 44 -- for insects 38, 44 -- X-ASG-Orig-Subj: RE: [Microscopy] viaWWW:SEM - Need help with which scope to use for 38, 44 -- insects 38, 44 -- Thread-Index: AQHPks+SnFVPj2rqs0ethRN3K/NQYpuJxRqE 38, 44 -- Date: Mon, 30 Jun 2014 15:25:31 +0000 38, 44 -- Message-ID: {89AF5EEF4C4B8943A8D091D28859F7C898DC7161-at-EXCH04.hscnet.hsc.usf.edu} 38, 44 -- References: {201406281250.s5SCofLd017711-at-ns.microscopy.com} 38, 44 -- In-Reply-To: {201406281250.s5SCofLd017711-at-ns.microscopy.com} 38, 44 -- Accept-Language: en-US 38, 44 -- Content-Language: en-US 38, 44 -- X-MS-Has-Attach: 38, 44 -- X-MS-TNEF-Correlator: 38, 44 -- x-originating-ip: [10.119.4.53] 38, 44 -- Content-Type: text/plain; charset="utf-8" 38, 44 -- MIME-Version: 1.0 38, 44 -- X-Barracuda-Connect: UNKNOWN[10.119.4.42] 38, 44 -- X-Barracuda-Start-Time: 1404141932 38, 44 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-mod/mark.cgi 38, 44 -- X-Virus-Scanned: by bsmtpd at health.usf.edu 38, 44 -- X-Barracuda-Spam-Score: 0.00 38, 44 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_SC0_MISMATCH_TO 38, 44 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.7105 38, 44 -- Rule breakdown below 38, 44 -- pts rule name description 38, 44 -- ---- ---------------------- -------------------------------------------------- 38, 44 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header 38, 44 -- Content-Transfer-Encoding: 8bit 38, 44 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id s5UFPYck015492 ==============================End of - Headers==============================
Through the efforts of our Hitachi service engineer, we will have a needle valve installed tomorrow. Thanks to everyone for your helpful suggestions.
Dan Fairweather Senior Materials Development Engineer Advanced Materials, Dept. 32-22
-----Original Message----- X-from: Philip Oshel [mailto:oshel1pe-at-cmich.edu] Sent: Monday, June 30, 2014 11:29 AM To: Fairweather, Dan
Years ago, we had done some work with Roy Larimer of MicroOptics. It looks like Visionary Digital is now selling his BK set-up.
http://www.visionarydigital.com/Pricing.html
The system was based on a clever use of image scanning. Also, he developed a special chamber for holding the bugs. The resulting bug images were amazing. I still have a copy of an app note we wrote and tried to locate the original website (2002!) but it now comes up in Japanese.
Check out the Visionary Digital site and see if this would be helpful.
Caveat: We have no commerical involvement/interest in this product.
Good Hunting!
Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through Fall 2014. Call us today for a free training evaluation.
At 02:37 PM 6/29/2014, you wrote:
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==============================Original Headers============================== 14, 28 -- From bfoster-at-the-mip.com Mon Jun 30 12:33:18 2014 14, 28 -- Received: from barcelona.directrouter.com (barcelona.directrouter.com [72.249.107.2]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5UHXIKh022866 14, 28 -- for {Microscopy-at-Microscopy.com} ; Mon, 30 Jun 2014 12:33:18 -0500 14, 28 -- Message-Id: {201406301733.s5UHXIKh022866-at-ns.microscopy.com} 14, 28 -- Received: from 99-168-105-118.lightspeed.rcsntx.sbcglobal.net ([99.168.105.118]:55562 helo=Bs-PC.the-mip.com) 14, 28 -- by barcelona.directrouter.com with esmtpsa (TLSv1:DHE-RSA-AES256-SHA:256) 14, 28 -- (Exim 4.82) 14, 28 -- (envelope-from {bfoster-at-the-mip.com} ) 14, 28 -- id 1X1fSA-0004VQ-3k 14, 28 -- for Microscopy-at-Microscopy.com; Mon, 30 Jun 2014 12:33:18 -0500 14, 28 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 28 -- Date: Mon, 30 Jun 2014 12:33:03 -0500 14, 28 -- To: Microscopy-at-Microscopy.com 14, 28 -- From: Barbara Foster {bfoster-at-the-mip.com} 14, 28 -- Subject: Re: [Microscopy] Re: viaWWW:SEM - Need help with which scope 14, 28 -- to use for 14, 28 -- Mime-Version: 1.0 14, 28 -- Content-Type: text/plain; charset="us-ascii" 14, 28 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 14, 28 -- X-AntiAbuse: Primary Hostname - barcelona.directrouter.com 14, 28 -- X-AntiAbuse: Original Domain - microscopy.com 14, 28 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 14, 28 -- X-AntiAbuse: Sender Address Domain - the-mip.com 14, 28 -- X-Get-Message-Sender-Via: barcelona.directrouter.com: authenticated_id: bfoster-at-the-mip.com 14, 28 -- X-Source: 14, 28 -- X-Source-Args: 14, 28 -- X-Source-Dir: ==============================End of - Headers==============================
From reasonable-meds10-at-charter.com Mon Jun 30 16:52:22 2014 Return-Path: {reasonable-meds10-at-charter.com} Received: from 68-186-61-252.dhcp.mdfd.or.charter.com (68-186-61-252.dhcp.mdfd.or.charter.com [68.186.61.252]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5ULqJms000700 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 30 Jun 2014 16:52:19 -0500 Message-Id: {201406302152.s5ULqJms000700-at-ns.microscopy.com} X-Vipre-Scanned: 0069CE860077CA0069CFD3-TDI
Barry; Lucky you! At Intel, barriers to protect electron microscopes from sprinklers were not permitted, because if the microscope was on fire, the sprinklers had to put it out. The building was considered more important than the microscope!
John Mardinly, ASU
-----Original Message----- X-from: barrylamb-at-meadtest.com [mailto:barrylamb-at-meadtest.com] Sent: Friday, June 27, 2014 6:18 AM To: John Mardinly
Hi Rosemary,
We had Perspex canopies made up for our SEMs, Auger system and SIMS machines to protect from direct sprinkler spray.
Regards, Barry
Barry Lamb Director Mead Testing Ltd Registered address: 25 Mead Park,River Way, Harlow, Essex, CM20 2SE, UK Company no. 4572600 barrylamb-at-meadtest.com www.meadtest.com tel: + 44 (0) 1279 635864 fax: +44 (0) 1279 635874
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: 27 June 2014 13:21 To: barrylamb-at-meadtest.com
Hi Rosemary
Out of interest I have dealt with water cascading over microscopes, yes it has happened twice in my 49 electron microscopy years. As a service technician the main problem is not so much the water, but the debris that the water beings down onto the electrical circuits. Both of the instruments in question were running on vacuum only and managed to keep going overnight until security found the water running through the particular laboratories. One was a tap exploding, shooting a fountain of water into the EM room, the other the result of putting out a fire three levels above.
A third situation occurred when a water pipe came off one instrument in a basement EM unit, flooding the complete area to about 6 inches. Everyone of the instruments kept going, but as I arrived a technician with rubber boots and a long wooden pole was taking care of switching the most endangered instruments off. However this was in the 1980s when most EM Units were underground; the one I mentioned was also used as a Tornado shelter!
EM Units and water, great fun!
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Sent: 26 June 2014 11:09 To: protrain-at-emcourses.com
Dear Roger and Listers,
I have had the joy of a sprinkler system waterfall originally just outside my TEM lab in the hallway a few years back. I say originally for the water shortly flooded through the my space and several other labs and offices. Hence the several inches of water was not over but under our scope. Thankfully the scope was not damaged but the mess took several days to clean up.
I have lived long enough that I DID experience a TEM full of water. It was a Phillips 200 (1966 version) in the Biology Department at the University of Pennsylvania. When I went on the scope and looked in the binoculars it looked very strange. Water was in and on the outside of the column. The water was not near the filament but I immediately turned it off!!! One does not think to see if the column is flooded on a regular basis. This was before Phillips became FEI but I do not remember the year, maybe 1978? Two very talented service engineers spent about a week, if I remember correctly, taking the column apart, drying each part out and putting it back together. Several years later I found water on the outside of the column in the crack where two lenses joined together but I caught it at the beginning and shut down the water supply immediately. An O-ring had broken where the hose was clamped into the lens, an easy fix. This old scope outlived several of its service engineers and was turned off for the last time in 2005.
Oh! I had also found that when my beam would pulse with a power instability, it was not the fault of the filament or a bad vacuum tube or a transistor but the SPARK PLUG. The very small gap would have a fleck of carbon blocking it. After I ran a piece of paper through to open the gap all would be fine for the next eight years or so.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Pat
Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491
================== On 6/28/14 9:07 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} X-from: roger.ristau-at-uconn.edu () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } -------------------------------------------------------------------------- } - } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both roger.ristau-at-uconn.edu as well as the Microscopy } Listserver } -------------------------------------------------------------------------- } - } } Email: roger.ristau-at-uconn.edu } Name: Roger Ristau } } Organization: University of Connecticut } } Title-Subject: [Filtered] Re: Sprinklers in instrument rooms } } Message: Here is a situation that I bet not many have encountered: We had } a TEM which developed a } leak in lens cooling with the result that the INSIDE of the TEM filled } with water. (I was not here } but was told the camera/viewing chamber looked like "a fish bowl.") } } My predecessor, Larry McCurdy, courageously attacked the mess and } restored the TEM to service! It } soldiered on for many years. } } Point of story: Flooding the OUTSIDE of the TEM may actually be more } harmful (electrical } shorts,etc.) than flooding the INSIDE! } } Cheers } Roger } } Institute of Materials Science } University of Connecticut
==============================Original Headers============================== 11, 34 -- From connellyps-at-nhlbi.nih.gov Mon Jun 30 18:33:11 2014 11, 34 -- Received: from nihxway5out.hub.nih.gov (nihxway5out.hub.nih.gov [128.231.90.113]) 11, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s5UNX9lJ011763 11, 34 -- for {microscopy-at-microscopy.com} ; Mon, 30 Jun 2014 18:33:10 -0500 11, 34 -- X-IronPortListener: Outbound_SMTP 11, 34 -- X-IronPort-Anti-Spam-Filtered: true 11, 34 -- X-IronPort-Anti-Spam-Result: AgsFAAfzsVOcKEe0/2dsb2JhbABXA4MNUlrFD1OBERZ1hAMBAgQSKD8SAQgNCh8vEx0KBA4FIoggDaEzplYXjiURAQI+EAILC4QyBZAjijuHL4Q7iBODQmyBCzk 11, 34 -- X-IPAS-Result: AgsFAAfzsVOcKEe0/2dsb2JhbABXA4MNUlrFD1OBERZ1hAMBAgQSKD8SAQgNCh8vEx0KBA4FIoggDaEzplYXjiURAQI+EAILC4QyBZAjijuHL4Q7iBODQmyBCzk 11, 34 -- Received: from unknown (HELO CASHTV17.nih.gov) ([156.40.71.180]) 11, 34 -- by nihxway5out.hub.nih.gov with ESMTP/TLS/AES128-SHA; 30 Jun 2014 19:33:09 -0400 11, 34 -- Received: from MLBXV14.nih.gov ([169.254.4.101]) by CASHTV17.nih.gov 11, 34 -- ([156.40.71.180]) with mapi id 14.03.0174.001; Mon, 30 Jun 2014 19:33:09 11, 34 -- -0400 11, 34 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 11, 34 -- To: MSA {microscopy-at-microscopy.com} 11, 34 -- CC: "roger.ristau-at-uconn.edu" {roger.ristau-at-uconn.edu} 11, 34 -- Subject: Re: [Microscopy] viaWWW:Sprinklers in instrument rooms and Beam 11, 34 -- fluctuation 11, 34 -- Thread-Topic: [Microscopy] viaWWW:Sprinklers in instrument rooms and Beam 11, 34 -- fluctuation 11, 34 -- Thread-Index: AQHPlLul+M/1ts6tzEW2uIW5X3inHQ== 11, 34 -- Date: Mon, 30 Jun 2014 23:33:07 +0000 11, 34 -- Message-ID: {CFD75DCF.4016%connellyps-at-mail.nih.gov} 11, 34 -- Accept-Language: en-US 11, 34 -- Content-Language: en-US 11, 34 -- X-MS-Has-Attach: 11, 34 -- X-MS-TNEF-Correlator: 11, 34 -- user-agent: Microsoft-MacOutlook/14.4.3.140616 11, 34 -- x-originating-ip: [156.40.71.191] 11, 34 -- Content-Type: text/plain; charset="us-ascii" 11, 34 -- Content-ID: {630CD68266C5A440A26668F1781EAD83-at-mail.nih.gov} 11, 34 -- MIME-Version: 1.0 11, 34 -- Content-Transfer-Encoding: 8bit 11, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s5UNX9lJ011763 ==============================End of - Headers==============================
From drugs_reasonable14-at-rr.com Mon Jun 30 20:36:43 2014 Return-Path: {drugs_reasonable14-at-rr.com} Received: from Randy-PC.nyc.rr.com (cpe-74-72-138-2.nyc.res.rr.com [74.72.138.2] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s611afro013037 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 30 Jun 2014 20:36:41 -0500 Message-Id: {201407010136.s611afro013037-at-ns.microscopy.com}
I wonder if anybody has explored the "Rocking" mode available in the ASID (scanning) panel on a JEM-2100 LaB6 or similar instrument. I can get nice channeling patterns using the backscatter detector; also some corresponding Kikuchi diffraction with the STEM detector. The software allows control of both working distance and camera length. But there is essentially no documentation on this beyond "Channeling pattern observation mode". It is not entirely clear to me what the actual ray diagram is here, or how it differs from normal STEM mode. Should the probe be rocking about a fixed point? The signal looks like a channeling pattern superimposed on an image. I'd especially like to do electron channeling contrast imaging.
Please let me know if you have any tips. Thanks. ------------------------------------------ Phil Ahrenkiel, Associate Professor Nanoscience and Nanoengineering South Dakota School of Mines and Technology 501 E. Saint Joseph St. Rapid City, SD 57701 U.S.A. Office: EP 221 Phone: 605-394-5238, Cell: 720-988-6627 Email: Phil.Ahrenkiel-at-sdsmt.edu
==============================Original Headers============================== 5, 37 -- From Phil.Ahrenkiel-at-sdsmt.edu Tue Jul 1 18:01:54 2014 5, 37 -- Received: from silver.sdsmt.edu (silver.sdsmt.edu [151.159.1.1]) 5, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s61N1r2K004330 5, 37 -- for {Microscopy-at-Microscopy.Com} ; Tue, 1 Jul 2014 18:01:53 -0500 5, 37 -- Received: from SDSMT-ex13.sdsmt.edu (sdsmt-ex13.sdsmt.edu [151.159.3.13]) 5, 37 -- by silver.sdsmt.edu (8.14.9/8.14.7) with ESMTP id s61N1rJ7022715 5, 37 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=OK) 5, 37 -- for {Microscopy-at-Microscopy.Com} ; Tue, 1 Jul 2014 17:01:53 -0600 5, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sdsmt.edu; 5, 37 -- s=sdsmtdkim; t=1404255713; 5, 37 -- bh=myCfBXNzyNlvhnU0IIVQxycswHpH624TrSFfjeYdlsY=; 5, 37 -- h=From:To:Subject:Date; 5, 37 -- b=C5gsvqid2gDMKSNM7+W6UqbpUzSN/ElNhPits8/rgMJ6k7swTz61lFaXxBdqcM9T3 5, 37 -- r9Fcw+j7AQeQMwEMeBj15T4i9919fMhr8M9t8+8NcqBOIVRRhMJ3fnS8ot/a9/W8Tk 5, 37 -- CfWcCIM9/9BuCl5xHaYKWRNgPRnj5arF9j/sD1zk= 5, 37 -- Received: from SDSMT-ex13.sdsmt.edu (2607:f558:1000:f::6d) by 5, 37 -- SDSMT-ex13.sdsmt.edu (2607:f558:1000:f::6d) with Microsoft SMTP Server (TLS) 5, 37 -- id 15.0.847.32; Tue, 1 Jul 2014 17:01:52 -0600 5, 37 -- Received: from SDSMT-ex13.sdsmt.edu ([fe80::1492:416b:26a0:40e8]) by 5, 37 -- SDSMT-ex13.sdsmt.edu ([fe80::1492:416b:26a0:40e8%15]) with mapi id 5, 37 -- 15.00.0847.030; Tue, 1 Jul 2014 17:01:52 -0600 5, 37 -- From: "Ahrenkiel, Phil" {Phil.Ahrenkiel-at-sdsmt.edu} 5, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 5, 37 -- Subject: rocking on a JEM-2100 5, 37 -- Thread-Topic: rocking on a JEM-2100 5, 37 -- Thread-Index: Ac+VgHD4jJPp1IMbQfKPHCR26AjS9Q== 5, 37 -- Date: Tue, 1 Jul 2014 23:01:51 +0000 5, 37 -- Message-ID: {26f701a3f34a4cc58a79cf540259dc0b-at-SDSMT-ex13.sdsmt.edu} 5, 37 -- Accept-Language: en-US 5, 37 -- Content-Language: en-US 5, 37 -- X-MS-Has-Attach: 5, 37 -- X-MS-TNEF-Correlator: 5, 37 -- x-originating-ip: [151.159.10.162] 5, 37 -- Content-Type: text/plain; charset="us-ascii" 5, 37 -- MIME-Version: 1.0 5, 37 -- Content-Transfer-Encoding: 8bit 5, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s61N1r2K004330 ==============================End of - Headers==============================
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Email: js51-at-princeton.edu Name: John Schreiber
Organization: Princeton Univ
Title-Subject: [Filtered] LAB6 filament
Message: Hello Rashmi,
You can check that the support of the LAB6 crystal is intact. Look at where the material that holds the crystal in place is intact and does not have cracks. The cracks will cause beam instabilities.
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Jul 1 20:18:18 2014 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s621II49026146 11, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:18:18 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 7A7F87245E7 11, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:18:18 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id U8MmvWlckaa9 for {microscopy-at-microscopy.com} ; 11, 28 -- Tue, 1 Jul 2014 20:18:11 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 0B8027245DA 11, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:18:11 -0500 (CDT) 11, 28 -- Message-ID: {53B35DD2.60001-at-microscopy.com} 11, 28 -- Date: Tue, 01 Jul 2014 20:18:10 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:LAB6 filament 11, 28 -- References: {201406301609.s5UG9Y96002781-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201406301609.s5UG9Y96002781-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201406301609.s5UG9Y96002781-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Optimum operating kV for thin section biological samples
Message: What is the optimum operating kV for ultrathin (60-80nm)sections of biological tissue cells prepared from LR White, the Lowicryls, Embed 812 on a scope with digital imaging capabilities?
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==============================Original Headers============================== 10, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Jul 1 20:19:52 2014 10, 28 -- Received: from znl.com ([206.69.208.20]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s621JpA0027024 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:19:51 -0500 10, 28 -- Received: from localhost (localhost [127.0.0.1]) 10, 28 -- by znl.com (Postfix) with ESMTP id A551B724602 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:19:51 -0500 (CDT) 10, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 28 -- Received: from znl.com ([127.0.0.1]) 10, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id elyY8jNDdUBq for {microscopy-at-microscopy.com} ; 10, 28 -- Tue, 1 Jul 2014 20:19:47 -0500 (CDT) 10, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 10, 28 -- by znl.com (Postfix) with ESMTPA id 1F0F37245F5 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:19:47 -0500 (CDT) 10, 28 -- Message-ID: {53B35E33.5000905-at-microscopy.com} 10, 28 -- Date: Tue, 01 Jul 2014 20:19:47 -0500 10, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 10, 28 -- MIME-Version: 1.0 10, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 28 -- Subject: viaWWW:Optimum operating kV for thin section biological samples 10, 28 -- References: {201406301948.s5UJm301017009-at-ns.microscopy.com} 10, 28 -- In-Reply-To: {201406301948.s5UJm301017009-at-ns.microscopy.com} 10, 28 -- X-Forwarded-Message-Id: {201406301948.s5UJm301017009-at-ns.microscopy.com} 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: Crothall would like to host a microscopy certification class for a group of their technicians. Please get in touch with me so we can talk about putting together such a class if you are interested. Thanks so much.
Jodie
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Jul 1 20:20:53 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s621Knse028863 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:20:51 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 110FD724611 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:20:49 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id h2o0vOk25H+A for {microscopy-at-microscopy.com} ; 13, 28 -- Tue, 1 Jul 2014 20:20:44 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 64A93724604 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:20:44 -0500 (CDT) 13, 28 -- Message-ID: {53B35E6C.90701-at-microscopy.com} 13, 28 -- Date: Tue, 01 Jul 2014 20:20:44 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Microscope Training 13, 28 -- References: {201407011337.s61Dbt9V022929-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201407011337.s61Dbt9V022929-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201407011337.s61Dbt9V022929-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: thomas.beck-at-novelis.com Name: TJ Beck
Organization: Novelis
Title-Subject: [Filtered] Detector info for DTSA II
Message: Has anyone had any luck obtaining detector info from Oxford to fill out the detector preferences in DTSA II?
Thanks,
TJ
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Jul 1 20:21:34 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s621LW5l029278 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:21:33 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 1EA79724620 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:21:32 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id crl7POondT14 for {microscopy-at-microscopy.com} ; 14, 28 -- Tue, 1 Jul 2014 20:21:27 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 58B37724613 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 1 Jul 2014 20:21:27 -0500 (CDT) 14, 28 -- Message-ID: {53B35E97.3010903-at-microscopy.com} 14, 28 -- Date: Tue, 01 Jul 2014 20:21:27 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Detector info for DTSA II 14, 28 -- References: {201407011915.s61JFQQl000569-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201407011915.s61JFQQl000569-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201407011915.s61JFQQl000569-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: peter.miller-at-monash.edu Name: Peter Miller
Organization: Monash Centre for Electron Microscopy, Monash University
Title-Subject: [Filtered] Re: Two fixed-term scanning electron microscopist positions
Message: The Monash Centre for Electron Microscopy is seeking outstanding candidates for two replacement Scanning Electron Microscopists. Both positions are located at the Clayton campus.
The Monash Centre for Electron Microscopy conducts research and provides advanced instrumentation, training and expertise in electron microscopy. The Centre undertakes and facilitates research projects across a range of disciplines in the physical sciences and engineering and serves researchers from Monash University, other universities, government research agencies and industry.
The Centre has a world-class suite of instrumentation housed in a dedicated, high-stability building designed to optimise instrument performance. You will provide expertise and training in scanning electron microscopy to support the research activities of researchers using the Centre.
The successful applicants will have advanced understanding and skills in the acquisition and interpretation of SEM data and its application to the solution of research problems in the physical sciences. These positions are maternity leave replacements and will be for a minimum duration of 6 months.
Appointments will be made at the level appropriate to your experience and qualifications. Please address the appropriate selection criteria according to your skills and qualifications.
These roles are full-time positions; however, flexible working arrangements may be negotiated.
For details see http://www.monash.edu.au/jobs/, ref. Electron Microscopist Job 525148
Enquiries: Professor Joanne Etheridge, Director, Monash Centre for Electron Microscopy, +61 3 9905 1836, joanne.etheridge-at-monash.au
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Dear Dr White, my lab is also recently looking into building a new lab to house the Aberration Corrected TEM. We are also in the discussion whether we can do away the fire sprinkler. One proposal (which is not yet finalized) is to have a two-step solution. First is to have a pre-action system (plus sprinkler), second is to have a FM-200 waterless fire suppression systems. So if fire started,the FM-200 will be activated, after sometime if it didn't put out the fire, sprinkler will comes in (correct me if i am wrong in the procedure). However, in Smy country, we have various regulatories on this fire suppression system which we need to deal with before we consider viable. Another problem is the cost. So i am not sure if Australia allows.
Best Regards, Yee Yan FACTS Lab Nanyang Technological University
On Sunday, 29 June 2014, 16:21, "Rosemary.White-at-csiro.au" {Rosemary.White-at-csiro.au} wrote:
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FYI a summary of responses. Thanks much to all, very useful.
1. Alternatives are available, but expensive. These include: - firewalls around each room lacking sprinklers, and firedoor entryway - rather expensive. The walls and door have to be able to resist fire for 1 hour. - gas systems - also expensive, and in Australia these are apparently only allowed in non-occupied rooms (I am told, not going to fight it, save fight for other stuff...).
So
2. Accept the inevitability of sprinklers, and insist on the following: - sprinkler head(s) as far from instrument as possible, esp. from main power supplies - sprinkler heads enclosed in wire cages to prevent accidental knocking when moving large items in the room
3. Realise that floods from pipes/sinks/rain events are much more likely than sprinkler activation, so: - install drip pans above the sprinklers, either below the ceiling or within it, to deflect potential floods from above away from the instrument - raise important components at least several cm above floor level - have a system that only fills the sprinkler pipes when the sprinkler(s) are activated to avoid slow leaks (drips)
4. Also realise that sprinkler pipes may be sources of conductive interference, so: - ask that pipes are empty unless sprinkler(s) activated - insert a rubber or other non-metallic connection between the main sprinkler supply system and the sprinklers in instrument rooms
and also, check your insurance policy!
Like others, the only floods I've experienced were from other sources - in a previous institution on one torrential weekend several staff came in to check for leaks and ended up doing a bucket brigade from the EM unit in the basement. All fine, except for the piles of paperwork around desks...
And Roger Ristau's email reminded me that about 15 years ago the in lens cooling system in our JEOL 6400 developed a leak and flooded the lens, detected by water dripping down into the chamber - messy! The old girl is still chugging along, though not for much longer - the old electronic boards are cracking and won't survive the move, so turning off for good in October. :(
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry (for 1 more day) GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
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I'd like to plastic embed samples of minced meat for LM and TEM analysis. My fear is that the mince will dissipate when put in glutaraldehyde for fixation. Do you have any experience or suggestion of how to keep the mince structure intact during the fixation, dehydration and infiltration steps?
Best regards, Sofia
............................. Dr Sofia Kihlman Řiseth Research Scientist
CSIRO Food and Nutrition Flagship E sofia.oiseth-at-csiro.au T +61 3 9731 3541 671 Sneydes road, Werribee, VIC 3030, Australia www.csiro.au
From drugs-affordable10-at-covenantescrow.com Thu Jul 3 03:00:59 2014 Return-Path: {drugs-affordable10-at-covenantescrow.com} Received: from smtp1.covenantescrow.com ([92.39.102.160]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6380uZc011969 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 3 Jul 2014 03:00:57 -0500 Message-Id: {201407030800.s6380uZc011969-at-ns.microscopy.com}
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA ERRC
Title-Subject: [Filtered] denatured EtOH
Message: Is denatured ethanol sufficient for dehydrating samples for SEM & TEM? Or is absolute required? There is quite a price difference, as well as ordering hurdles.
thanks
Joe
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==============================Original Headers============================== 21, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jul 3 06:46:06 2014 21, 28 -- Received: from znl.com ([206.69.208.20]) 21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s63Bk5UB018380 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2014 06:46:05 -0500 21, 28 -- Received: from localhost (localhost [127.0.0.1]) 21, 28 -- by znl.com (Postfix) with ESMTP id DC3AD725792 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2014 06:46:05 -0500 (CDT) 21, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 21, 28 -- Received: from znl.com ([127.0.0.1]) 21, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 21, 28 -- with ESMTP id y+oBy5paOmXj for {microscopy-at-microscopy.com} ; 21, 28 -- Thu, 3 Jul 2014 06:46:01 -0500 (CDT) 21, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 21, 28 -- by znl.com (Postfix) with ESMTPA id 09761725780 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2014 06:46:01 -0500 (CDT) 21, 28 -- Message-ID: {53B54278.4020804-at-microscopy.com} 21, 28 -- Date: Thu, 03 Jul 2014 06:46:00 -0500 21, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 21, 28 -- MIME-Version: 1.0 21, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 28 -- Subject: viaWWW:denatured EtOH 21, 28 -- References: {201407021821.s62ILdlB002129-at-ns.microscopy.com} 21, 28 -- In-Reply-To: {201407021821.s62ILdlB002129-at-ns.microscopy.com} 21, 28 -- X-Forwarded-Message-Id: {201407021821.s62ILdlB002129-at-ns.microscopy.com} 21, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 21, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: pfraundorf-at-umsl.edu Name: Phil Fraundorf
Organization: University of Missouri St. Louis
Title-Subject: [Filtered] TEM: javascript electron optics?
Message: Perhaps for the first time, HTML with javascript FFT's is fast enough on many platforms to do some interesting stuff in real time. In particular, we've been looking at pedagogical uses for JS/HTML5 in strong phase-object wave-propagation & Fourier-window darkfield analysis.
This link* illustrates ways these might be used e.g. to provide microscopy students with on-line exercises in focus-adjustment and data-acquisition, as well as in study of contrast-transfer and image periodicities.
Unlike plugins for ImageJ, routines in JS/HTML5 are not likely to be much use for analyzing your own data. Local programs are generally needed for that. However they do hold promise for giving folks visceral experience with key microscopy challenges. In this context, how might freely-available web resources like this be re-arranged so as to be useful to folks learning to set up, and analyze, images in your lab?
P. Fraundorf UM-StL Physics & Astronomy/CNS
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Joe, We run a large number of both TEM and SEM experiments and have always used pint sized (open just before 100% step) Ethyl Alcohol 200 proof absolute andydrous ACS/USP grade form Pharmco-AAPER #64-17-5. We use the opened bottles to make ethanol parts and general lab use. It is crucial that the ethanol contain no water. In the old days I used to molecular sieve the ethanol, but it is no longer necessary. Good luck; Michael Delannoy
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, July 03, 2014 8:20 AM To: mdelann1-at-jhmi.edu
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA ERRC
Title-Subject: [Filtered] denatured EtOH
Message: Is denatured ethanol sufficient for dehydrating samples for SEM & TEM? Or is absolute required? There is quite a price difference, as well as ordering hurdles.
thanks
Joe
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
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==============================Original Headers============================== 21, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jul 3 06:46:06 2014 21, 28 -- Received: from znl.com ([206.69.208.20]) 21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s63Bk5UB018380 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2014 06:46:05 -0500 21, 28 -- Received: from localhost (localhost [127.0.0.1]) 21, 28 -- by znl.com (Postfix) with ESMTP id DC3AD725792 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2014 06:46:05 -0500 (CDT) 21, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 21, 28 -- Received: from znl.com ([127.0.0.1]) 21, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 21, 28 -- with ESMTP id y+oBy5paOmXj for {microscopy-at-microscopy.com} ; 21, 28 -- Thu, 3 Jul 2014 06:46:01 -0500 (CDT) 21, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 21, 28 -- by znl.com (Postfix) with ESMTPA id 09761725780 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2014 06:46:01 -0500 (CDT) 21, 28 -- Message-ID: {53B54278.4020804-at-microscopy.com} 21, 28 -- Date: Thu, 03 Jul 2014 06:46:00 -0500 21, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 21, 28 -- MIME-Version: 1.0 21, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 28 -- Subject: viaWWW:denatured EtOH 21, 28 -- References: {201407021821.s62ILdlB002129-at-ns.microscopy.com} 21, 28 -- In-Reply-To: {201407021821.s62ILdlB002129-at-ns.microscopy.com} 21, 28 -- X-Forwarded-Message-Id: {201407021821.s62ILdlB002129-at-ns.microscopy.com} 21, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 21, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Joe: You should always dehydrate using pure ethanol, starting at 25 or 50%, then continue with these changes: 65%, 80%, 95%, 3x 100% then if you are using an epoxy resin transition into that. Absolute ethanol is necessary for the 100% changes. Karen Bentley University of Rochester Medical Center
________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, July 03, 2014 8:01 AM To: Bentley, Karen
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA ERRC
Title-Subject: [Filtered] denatured EtOH
Message: Is denatured ethanol sufficient for dehydrating samples for SEM & TEM? Or is absolute required? There is quite a price difference, as well as ordering hurdles.
thanks
Joe
Login Host: 199.133.43.77 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
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==============================Original Headers============================== 21, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jul 3 06:46:06 2014 21, 28 -- Received: from znl.com ([206.69.208.20]) 21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s63Bk5UB018380 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2014 06:46:05 -0500 21, 28 -- Received: from localhost (localhost [127.0.0.1]) 21, 28 -- by znl.com (Postfix) with ESMTP id DC3AD725792 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2014 06:46:05 -0500 (CDT) 21, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 21, 28 -- Received: from znl.com ([127.0.0.1]) 21, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 21, 28 -- with ESMTP id y+oBy5paOmXj for {microscopy-at-microscopy.com} ; 21, 28 -- Thu, 3 Jul 2014 06:46:01 -0500 (CDT) 21, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 21, 28 -- by znl.com (Postfix) with ESMTPA id 09761725780 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2014 06:46:01 -0500 (CDT) 21, 28 -- Message-ID: {53B54278.4020804-at-microscopy.com} 21, 28 -- Date: Thu, 03 Jul 2014 06:46:00 -0500 21, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 21, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 21, 28 -- MIME-Version: 1.0 21, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 28 -- Subject: viaWWW:denatured EtOH 21, 28 -- References: {201407021821.s62ILdlB002129-at-ns.microscopy.com} 21, 28 -- In-Reply-To: {201407021821.s62ILdlB002129-at-ns.microscopy.com} 21, 28 -- X-Forwarded-Message-Id: {201407021821.s62ILdlB002129-at-ns.microscopy.com} 21, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 21, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From reasonable-medications10-at-lapme.com Fri Jul 4 06:11:35 2014 Return-Path: {reasonable-medications10-at-lapme.com} Received: from mail3.lapme.com ([5.140.162.12]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s64BBVRq011423 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 4 Jul 2014 06:11:32 -0500 Message-Id: {201407041111.s64BBVRq011423-at-ns.microscopy.com}
from: tomw-at-uidaho.edu ()
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I have a user who collected TEM/EDS data on a 4pi RevolutionEDX system. We need to (if possible) open and evaluate the spectrums. No access to the RevEDX software itself....obviously, and the 4pi website wasn't much help. Having no luck reading the files with software we have on hand. I've tried DTSA-II with no luck??
Any suggestions or advice would be greatly appreciated.
Thanks!
Tom
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From reasonable-meds1-at-spbmts.ru Sun Jul 6 07:47:31 2014 Return-Path: {reasonable-meds1-at-spbmts.ru} Received: from host-52-158-66-217.spbmts.ru (host-52-158-66-217.spbmts.ru [217.66.158.52]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s66ClRf8008470 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 6 Jul 2014 07:47:28 -0500 Message-Id: {201407061247.s66ClRf8008470-at-ns.microscopy.com}
All,
Come to beautiful Tasmania for AMAS XIII, the 13th biennial Australian Microbeam Analysis Symposium, which will be held in Hobart on 11-13 February 2015, with Pre-Meeting Workshops 9-10 Feb, 2015.
The AMAS symposia provide a forum to discuss and share ideas on advances, trends and challenges in microanalysis and imaging with national and international leaders in the field, with an emphasis on practical solutions and applications.
Continuing the successful format of previous editions, AMAS XIII will be hosted at University venues and exclusively have oral presentations in a single session over three days, thus providing an intimate environment for in-depth discussions.
We invite you to present your research on technological developments and applications in a wide range of microscopy and microanalysis techniques.
A range of exciting invited speakers and workshops have already been confirmed.
For more information, see our website
http://microscopy.org.au/amas/amas13/
or talk to me or one of the other Aussies at M&M/IUMAS6 in Hartford.
Many thanks,
Karsten
AMAS XIII Co-Chair
Dr Karsten Goemann Research Fellow, Electron Microscopy & X-Ray Microanalysis Central Science Laboratory, University of Tasmania Mail: Private Bag 74, Hobart TAS 7001, Australia Location: Rooms 254-256, Chemistry Building, Dobson Road, Sandy Bay TAS 7005, Australia T: +61 (0)3 6226 2146 | F: +61 (0)3 6226 2494 | M: +61 (0)407 101 990 www.utas.edu.au/research/central-science-laboratory CRICOS 00586B
==============================Original Headers============================== 14, 32 -- From Karsten.Goemann-at-utas.edu.au Mon Jul 7 01:36:07 2014 14, 32 -- Received: from mx2.utas.edu.au (ironport.its.utas.edu.au [131.217.6.11]) 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s676a6CY011471 14, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Jul 2014 01:36:06 -0500 14, 32 -- X-IronPort-AV: E=Sophos;i="5.01,616,1399989600"; 14, 32 -- d="scan'208";a="113987945" 14, 32 -- Received: from unknown (HELO mmp-sby1.its.utas.edu.au) ([10.10.3.71]) 14, 32 -- by ironport-outgoing.utas.edu.au with ESMTP; 07 Jul 2014 16:36:04 +1000 14, 32 -- MIME-version: 1.0 14, 32 -- Content-transfer-encoding: 7BIT 14, 32 -- Content-type: text/plain; CHARSET=US-ASCII 14, 32 -- Received: from ITSSBYHC3.utas.ad.internal ([10.10.3.51]) 14, 32 -- by mmp-sby1.its.utas.edu.au 14, 32 -- (Sun Java(tm) System Messaging Server 7u3-17.01 64bit (built Jun 8 2010)) 14, 32 -- with ESMTP id {0N8B00H0PX04OUE0-at-mmp-sby1.its.utas.edu.au} for 14, 32 -- Microscopy-at-Microscopy.com; Mon, 07 Jul 2014 16:36:04 +1000 (EST) 14, 32 -- Received: from MBXSBYN3.utas.ad.internal ([fe80::e9fe:2d6a:f1b4:f1f2]) 14, 32 -- by ITSSBYHC3.utas.ad.internal ([::1]) with mapi id 14.03.0169.001; Mon, 14, 32 -- 07 Jul 2014 16:36:04 +1000 14, 32 -- From: Karsten Goemann {Karsten.Goemann-at-utas.edu.au} 14, 32 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 14, 32 -- Subject: AMAS XIII First Circular 14, 32 -- Thread-topic: AMAS XIII First Circular 14, 32 -- Thread-index: AQHPma25VRIVEhyCFUKVv76zV2148A== 14, 32 -- Date: Mon, 07 Jul 2014 06:36:03 +0000 14, 32 -- Message-id: {7D5D1D1F-F0AC-45E4-82E4-6DCD71C45409-at-utas.edu.au} 14, 32 -- Accept-Language: en-AU, en-US 14, 32 -- Content-language: en-US 14, 32 -- X-MS-Has-Attach: 14, 32 -- X-MS-TNEF-Correlator: 14, 32 -- X-Originating-IP: [10.10.3.55] 14, 32 -- Content-id: {B4747B3C6AE33E469EEDA5430EDC26F7-at-postoffice.utas.edu.au} ==============================End of - Headers==============================
From cheapest_drugstore7-at-in-addr.arpa Mon Jul 7 07:13:45 2014 Return-Path: {cheapest_drugstore7-at-in-addr.arpa} Received: from user-22.81.118.217.in-addr.arpa (user-22.81.118.217.in-addr.arpa [217.118.81.22] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s67CDe0k013662 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 7 Jul 2014 07:13:41 -0500 Message-Id: {201407071213.s67CDe0k013662-at-ns.microscopy.com}
Getting the most from your AFM requires effective utilization of image processing software. This free, live-streaming seminar covers how to level images,
display images, and analyze images with AFM image processing software. The demonstration will utilize Gwyddion's open source software and be led by Paul West, PhD.
To RSVP for this 30 minute AFM Image Processing Seminar, visit AFMWorkshop's Ustream Channel: http://www.ustream.tv/channel/Atomic-Force-Microscopes
Thank you, Pamela Stone AFMWorkshop, Inc. 1434 E. 33rd Street Signal Hill, CA 90755 www.afmworkshop.com
==============================Original Headers============================== 7, 20 -- From pamela-at-afmworkshop.com Tue Jul 8 11:48:11 2014 7, 20 -- Received: from p3plwbeout23-05.prod.phx3.secureserver.net (p3plsmtp23-05-2.prod.phx3.secureserver.net [68.178.252.170]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s68GmBjQ006403 7, 20 -- for {microscopy-at-microscopy.com} ; Tue, 8 Jul 2014 11:48:11 -0500 7, 20 -- Received: from localhost ([68.178.252.243]) 7, 20 -- by p3plwbeout23-05.prod.phx3.secureserver.net with bizsmtp 7, 20 -- id PsoA1o0025Fr2Gc01soAmf; Tue, 08 Jul 2014 09:48:10 -0700 7, 20 -- X-SID: PsoA1o0025Fr2Gc01 7, 20 -- Received: (qmail 19352 invoked by uid 99); 8 Jul 2014 16:48:10 -0000 7, 20 -- Content-Type: text/plain; charset="utf-8" 7, 20 -- X-Originating-IP: 66.214.45.45 7, 20 -- User-Agent: Workspace Webmail 5.7.1 7, 20 -- Message-Id: {20140708094809.636791cbb6cfeca4bdfcf4f7edb54753.c65b050af3.wbe-at-email23.secureserver.net} 7, 20 -- From: {pamela-at-afmworkshop.com} 7, 20 -- To: Microscopy-at-microscopy.com 7, 20 -- Subject: AFM Image Processing Software Seminar 7, 20 -- Date: Tue, 08 Jul 2014 09:48:09 -0700 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s68GmBjQ006403 ==============================End of - Headers==============================
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Hi, Everyone-
We have two Optronics cameras on light microscopes, a MacroFire and a MagnaFire. One has a Sony chip and one a Kodak chip, and they have different software, and we like both, especially Picture Frame (MacroFire). But the MacroFire driver does not work past Windows XP service pack 3, and the MagnaFire does not work past Win XP service pack 1. Their respective computers are dying, and although we keep putting them on newer old computers, it is a losing battle. Has anyone come up with a hack to keep the older Optronics cameras going? I can sort of make them work with a generic twain driver, but we really like the capabiities of Picture Frame for mixing channels, etc. I heard a rumor they would work on Win 7 32 bit, but that didn't pan out. Any other solutions?
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Tue Jul 8 18:34:55 2014 4, 20 -- Received: from sf-v240.pbrc.hawaii.edu (sf-v240.pbrc.hawaii.edu [128.171.22.18]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s68NYtmR018430 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Jul 2014 18:34:55 -0500 4, 20 -- Received: from sf-v240.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id s68NYrGN001942 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Jul 2014 13:34:53 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by sf-v240.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id s68NYrJT001939 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Jul 2014 13:34:53 -1000 (HST) 4, 20 -- X-Authentication-Warning: sf-v240.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Tue, 8 Jul 2014 13:34:53 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-sf-v240 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: Optronics camera drivers 4, 20 -- Message-ID: {Pine.GSO.4.64.1407081326071.19275-at-sf-v240} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
From drugs-cheapest11-at-mts.ru Wed Jul 9 02:12:18 2014 Return-Path: {drugs-cheapest11-at-mts.ru} Received: from 172.gprs.mts.ru (172.gprs.mts.ru [213.87.136.172] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s697CF9O011059 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 9 Jul 2014 02:12:16 -0500 Message-Id: {201407090712.s697CF9O011059-at-ns.microscopy.com}
Hi Tina,
Have you tried Windows XP mode in Windows 7? Odds are that the Windows 7 version you have access to allows for this possibility. Windows XP mode was designed to allow for the use of legacy software in Windows 7. I am not sure how well it handles legacy hardware.
Some install info from Microsoft: http://windows.microsoft.com/en-us/windows7/install-and-use-windows-xp-mode-in-windows-7
best of luck
Rafael Buono Department of Botany University of Wisconsin - Madison
We're interested in purchasing an ion beam cross section polishing system to complement our other SEM and TEM sample preparation equipment. If you have experience with the systems from Gatan, Hitachi, JEOL, or Leica, could you please contact me offline to discuss the performance, reliability, ease of use, operating costs, and any other useful information which will help guide our purchase. Vendor responses are welcome as well.
Thank you for your help, Chris Senior Research Associate Nanoscale Characterization and Fabrication Laboratory Institute for Critical Technology and Applied Science Virginia Tech (540) 200-9511
==============================Original Headers============================== 3, 26 -- From microwink-at-gmail.com Wed Jul 9 12:23:52 2014 3, 26 -- Received: from mail-yh0-f49.google.com (mail-yh0-f49.google.com [209.85.213.49]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s69HNqsk025884 3, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Jul 2014 12:23:52 -0500 3, 26 -- Received: by mail-yh0-f49.google.com with SMTP id f73so2991341yha.8 3, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 09 Jul 2014 10:23:51 -0700 (PDT) 3, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=20120113; 3, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 26 -- bh=1ZRxl61GFxlYbUYj0ztZ4dHEe6z73Yn/aaP+YV110rc=; 3, 26 -- b=fVhIXfGQ3/vThoE6YTQDDqFca2yQcCrWD5CBmezpNdZvMUXiGGVehrZxNs4Ti0SI22 3, 26 -- XNAHmqRQkkXQcnP+0chgED7dKYp45PKiJWPJuNATBiubA/EWARdGjDodoczRyprgCJy7 3, 26 -- HDffiNhHxopZ6TL5ztxqp0L2Hpptu3nj6k5SGW49NuhkyW8/faPRTsJfk5KZNsDmQUli 3, 26 -- 0dW+Fs5gxWYikIANQdGqjObPxzfQrLaznMC7QpqFsMtx/Twzgw0NERLqWi+RHvAFWxr9 3, 26 -- 6JHRAJdbWRtBDyuJD5hB07yJh5UCkv+NVynvMGSGU+65W4QT8zH3C7qRGCGfqVzP85oh 3, 26 -- zSow== 3, 26 -- MIME-Version: 1.0 3, 26 -- X-Received: by 10.58.208.170 with SMTP id mf10mr40724094vec.22.1404926631625; 3, 26 -- Wed, 09 Jul 2014 10:23:51 -0700 (PDT) 3, 26 -- Received: by 10.220.133.129 with HTTP; Wed, 9 Jul 2014 10:23:51 -0700 (PDT) 3, 26 -- Date: Wed, 9 Jul 2014 13:23:51 -0400 3, 26 -- Message-ID: {CAA8T2POyOFdgyg1iyWyv+4Mog_=8QvQ4Tq0GiHzGBOQYxteeNg-at-mail.gmail.com} 3, 26 -- Subject: Experiences with ion beam cross section polishing systems 3, 26 -- From: Christopher Winkler {microwink-at-gmail.com} 3, 26 -- To: Microscopy-at-microscopy.com 3, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
The diaphragm pump in our plasma cleaner (Gatan Model 950)is failing, we are in need to find a replacement. This is a Pfeiffer MVP 020-3 DC membrane pump, anyone has suggested vendors in US we can purchase from?
Thank you in advance. Xinran Liu
________________________________________
Xinran Nick Liu, M.D. & Ph.D. Director of CCMI Electron Microscopy Core Facility Research Scientist in Cell Biology Yale University School of Medicine 333 Cedar Street, SHM I-E16A New Haven, CT 06520 Office: 203.785.4050 Lab: 203.785.5390 http://medicine.yale.edu/ccmi/em
From discounted_meds11-at-e-shoya.net Thu Jul 10 00:02:12 2014 Return-Path: {discounted_meds11-at-e-shoya.net} Received: from mail1.e-shoya.net (dsl218-207-Baku-AZ.connect.az [91.191.207.218] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6A52A4t017300 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 10 Jul 2014 00:02:11 -0500 Message-Id: {201407100502.s6A52A4t017300-at-ns.microscopy.com}
X-from: rashmi_mehata-at-yahoo.com ()
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] Three way valve and filament
Message: Dear All
Our 'FEI' HRTEM F-30 is down since over a month. There is a problem in 3 way valve in Zephyr Chiller. We would like hear from supplier who can supply us on urgent basis.
FEI TEM T-20 is down as the filament is blown off. Can we get the supplier in India who can supply the tungsten filament on urgent basis.
Regards Rashmi
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both susan.vanhorn-at-stonybrook.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: susan.vanhorn-at-stonybrook.edu Name: Susan C. Van Horn
Organization: Central Microscopy Imaging Center (C-MIC) Stony Brook University
Title-Subject: [Filtered] FEI service response time
Message: Hi - I was wondering what the response time has been for FEI TEM users for service calls - average time for your service reps to show up from the time you make a service call - thanks!! Sue
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Keep in mind that mere appearance of the representative does not yet mean a solution to your problem. While Average Response Time (ART) is a good indicator of responsiveness of the service organization, feedback on Mean Time To Repair (MTTR) could be even more valuable in evaluating its effectiveness.
When reviewing ART and MTTR from users and comparing there's feedback to your situation, take a note of either response comes from a customer under full "parts and labor" service contract or is it from Time and Materials (T&M) service call. Regardless of the particular service organization, wuite often there seem to be a significant difference in availability and quality of service available to full-contract and T&M customers.
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 7/10/2014 1:35 PM, zaluzec-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: susan.vanhorn-at-stonybrook.edu () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both susan.vanhorn-at-stonybrook.edu as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: susan.vanhorn-at-stonybrook.edu } Name: Susan C. Van Horn } } Organization: Central Microscopy Imaging Center (C-MIC) Stony Brook } University } } Title-Subject: [Filtered] FEI service response time } } Message: Hi - I was wondering what the response time has been for FEI } TEM users for service calls - average time for your service reps to show } up from the time you make a service call - thanks!! } Sue } } Login Host: 129.49.7.150 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- }
In addition to the option of purchasing electron sources from either Kimball Physics or Denka, you may consider refurbishing the old source by replacing filament and the tip. You can contact York Probe Sources in UK for tip rebuilding service.
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 7/10/2014 2:05 PM, wim.hagen-at-me.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Rashmi, } } Sometimes a good hit with a hammer works on the three-way valve. } It can also be opened and cleaned. } } Filaments can be ordered directly online at kymbalphysics.com. } } Best, } } Wim } } } On Jul 10, 2014, at 19:49, zaluzec-at-microscopy.com wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } X-from: rashmi_mehata-at-yahoo.com () } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying } } please copy both rashmi_mehata-at-yahoo.com as well as the Microscopy } } Listserver } } --------------------------------------------------------------------------- } } } } Email: rashmi_mehata-at-yahoo.com } } Name: Rashmi } } } } Organization: DBT } } } } Title-Subject: [Filtered] Three way valve and filament } } } } Message: } } Dear All } } } } Our 'FEI' HRTEM F-30 is down since over a month. There is a problem in 3 } } way valve in Zephyr Chiller. We would like hear from supplier who can } } supply us on urgent basis. } } } } FEI TEM T-20 is down as the filament is blown off. Can we get the } } supplier in India who can supply the tungsten filament on urgent basis. } } } } Regards } } Rashmi } } } } Login Host: 14.139.126.167 } } Listserver Email Form V - 20120416 } } --------------------------------------------------------------------------- } } } } } } -- } } =============================================== } } } } Do not reply to this messages it is from } } the Microscopy Listserver NO-REPLY forwarding } } system. You should send a new message to } } } } Microscopy-at-Microscopy.com } } } } ============================================= } } } } ==============================Original Headers============================== } } 15, 40 -- From nestor.zaluzec-at-gmail.com Thu Jul 10 12:33:54 2014 } } 15, 40 -- Received: from mail-pd0-f178.google.com (mail-pd0-f178.google.com [209.85.192.178]) } } 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6AHXsvK029181 } } 15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jul 2014 12:33:54 -0500 } } 15, 40 -- Received: by mail-pd0-f178.google.com with SMTP id r10so11055200pdi.23 } } 15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jul 2014 10:33:53 -0700 (PDT) } } 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 15, 40 -- d=gmail.com; s=20120113; } } 15, 40 -- h=sender:from:message-id:date:reply-to:user-agent:mime-version:to } } 15, 40 -- :subject:references:in-reply-to:content-type } } 15, 40 -- :content-transfer-encoding; } } 15, 40 -- bh=WWeVvnfOEUK1rIQcmBdRDi8tD+F9v4/u0TLRG7WGNdY=; } } 15, 40 -- b=mZ4uf0V1gj75yq3MJIF61mY6OB8z11oqlJHTwEGp5AuZJhSYyPl/lj+6j8U8VXXs0A } } 15, 40 -- oArfs65mdoYpvvn2nWT8MbgRJ2GWl8ZMMYy7wQt9D2Y9osj4ZqeFcm4HhooaJ0bqyYl4 } } 15, 40 -- a2qctUPYrkcP/P56EjTkVubAoWF2qCSph3jUrIDoaK+7XtT26+Bbp6IlOiI+iXfMdqqH } } 15, 40 -- v4nIzRioS4lrDlmHfiVRphoxDa0BkFMBJaMm9JZtzXXLTwAVRy9vjE2DpW+SijLjyXVZ } } 15, 40 -- a+R2X0C+tpHg5O6pPSGVR4GZRbuaDc5vaWnNzibEZnG0HBwui+J7NR/QMobHQKdzWilK } } 15, 40 -- ioEw== } } 15, 40 -- X-Received: by 10.70.131.5 with SMTP id oi5mr18532143pdb.69.1405013633345; } } 15, 40 -- Thu, 10 Jul 2014 10:33:53 -0700 (PDT) } } 15, 40 -- Received: from Nestors-MacBook-Pro-2014.local ([61.16.155.25]) } } 15, 40 -- by mx.google.com with ESMTPSA id z2sm8973725pdp.91.2014.07.10.10.33.51 } } 15, 40 -- for {microscopy-at-microscopy.com} } } 15, 40 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); } } 15, 40 -- Thu, 10 Jul 2014 10:33:52 -0700 (PDT) } } 15, 40 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} } } 15, 40 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} } } 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } } 15, 40 -- Message-ID: {53BECE7D.3090506-at-microscopy.com} } } 15, 40 -- Date: Thu, 10 Jul 2014 23:03:49 +0530 } } 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } } 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 } } 15, 40 -- MIME-Version: 1.0 } } 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 15, 40 -- Subject: viaWWW:Three way valve and filament for FEI instruments } } 15, 40 -- References: {201407091028.s69AScPB013409-at-ns.microscopy.com} } } 15, 40 -- In-Reply-To: {201407091028.s69AScPB013409-at-ns.microscopy.com} } } 15, 40 -- X-Forwarded-Message-Id: {201407091028.s69AScPB013409-at-ns.microscopy.com} } } 15, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 15, 40 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 6, 28 -- From wim.hagen-at-me.com Thu Jul 10 13:04:41 2014 } 6, 28 -- Received: from nk11p04mm-asmtp001.mac.com (nk11p04mm-asmtpout001.mac.com [17.158.236.236]) } 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6AI4f6u002921 } 6, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Jul 2014 13:04:41 -0500 } 6, 28 -- MIME-version: 1.0 } 6, 28 -- Content-transfer-encoding: 7BIT } 6, 28 -- Content-type: text/plain; CHARSET=US-ASCII } 6, 28 -- Received: from [192.168.2.125] } 6, 28 -- (dslb-084-057-197-141.pools.arcor-ip.net [84.57.197.141]) } 6, 28 -- by nk11p04mm-asmtp001.mac.com } 6, 28 -- (Oracle Communications Messaging Server 7u4-27.10(7.0.4.27.9) 64bit (built Jun } 6, 28 -- 6 2014)) with ESMTPSA id {0N8I0024GCVO8G10-at-nk11p04mm-asmtp001.mac.com} for } 6, 28 -- Microscopy-at-microscopy.com; Thu, 10 Jul 2014 18:04:40 +0000 (GMT) } 6, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure } 6, 28 -- engine=2.50.10432:5.12.52,1.0.14,0.0.0000 } 6, 28 -- definitions=2014-07-10_07:2014-07-10,2014-07-10,1970-01-01 signatures=0 } 6, 28 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 } 6, 28 -- suspectscore=15 phishscore=0 adultscore=0 bulkscore=0 classifier=spam adjust=0 } 6, 28 -- reason=mlx scancount=1 engine=7.0.1-1402240000 definitions=main-1407100202 } 6, 28 -- Subject: Re: [Microscopy] viaWWW:Three way valve and filament for FEI } 6, 28 -- instruments } 6, 28 -- References: {201407101749.s6AHnaJb025505-at-ns.microscopy.com} } 6, 28 -- From: Wim Hagen {wim.hagen-at-me.com} } 6, 28 -- X-Mailer: iPod touch Mail (11D201) } 6, 28 -- In-reply-to: {201407101749.s6AHnaJb025505-at-ns.microscopy.com} } 6, 28 -- Message-id: {DA1E98A3-DD6E-4E52-9329-F17D9154C2E4-at-me.com} } 6, 28 -- Date: Thu, 10 Jul 2014 20:04:32 +0200 } 6, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } ==============================End of - Headers============================== }
The Microscopy and Microanalysis Meeting (Aug. 3-7 2014 in Hartford, CT) is coming closer and I would like to make you aware that there are still openings in our Sunday Short Course on 3D electron microscopy (description below).It is not too late to register for it.
This course is ideal for people who need a comprehensive introduction or a refresher in 3D reconstruction methods and is suitable for biologists and material scientists. If you have already registered for the meeting you still can add the course registration as "extra item only".
Description:
X12 3D Electron Microscopy of Macromolecular Assemblies Teresa Ruiz, Michael Radermacher, Edward Morris 8:30 AM to 5:00 PM on Sunday, August 3.
Sample preparation: deep stain, vitreous ice Imaging conditions, low-dose imaging, tilt-pair data collection Alignment techniques and multivariate statistical analysis 3D reconstruction methods X-ray structure docking Techniques described have applications in both biological and material science
This short course will provide a comprehensive description of the methods used for 3D structure determination from electron micrographs of macromolecular complexes or weakly scattering specimens available in multiple copies.Specimen- preparation techniques for single particles (deep stain, vitreous ice) will be presented,followed by selection of optimal imaging conditions, including low-dose imaging. Next,a detailed explanation of image-processing techniques, with special emphasis on the random-conical reconstruction technique, will be presented. Finally, structure interpretation and docking of X-ray structures to 3D EM densities will be demonstrated.The techniques described could be applied to both biological and materials science specimens.
-- Michael Radermacher, Ph.D., Prof., FMSA University of Vermont Dept. Molecular Physiology and Biophysics HSRF 120 / 149 Beaumont Ave Burlington, VT 05405 USA
==============================Original Headers============================== 10, 19 -- From mraderma-at-uvm.edu Thu Jul 10 16:52:39 2014 10, 19 -- Received: from smtp2.uvm.edu (smtp2.uvm.edu [132.198.101.169]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6ALqd79030814 10, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Jul 2014 16:52:39 -0500 10, 19 -- Received: from webmail.uvm.edu (webmail3.uvm.edu [132.198.100.47]) 10, 19 -- by smtp2.uvm.edu (8.14.5/8.14.5) with ESMTP id s6ALqcEd022559 10, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Jul 2014 17:52:38 -0400 10, 19 -- Received: from egress.sbb.uvm.edu (egress.sbb.uvm.edu [132.198.90.91]) by 10, 19 -- webmail.uvm.edu (Horde Framework) with HTTP; Thu, 10 Jul 2014 17:52:38 -0400 10, 19 -- Date: Thu, 10 Jul 2014 17:52:38 -0400 10, 19 -- Message-ID: {20140710175238.Horde.gVOCe9iygh5TvwsmiWc2DrA-at-webmail.uvm.edu} 10, 19 -- From: Michael Radermacher {mraderma-at-uvm.edu} 10, 19 -- To: Microscopy-at-microscopy.com 10, 19 -- Subject: Sunday short course on 3D electron Microscopy at M&M 2014 10, 19 -- User-Agent: Internet Messaging Program (IMP) H4 (5.0.21) 10, 19 -- X-Authenticated-Sender: mraderma 10, 19 -- Content-Type: text/plain; charset=utf-8; format=flowed; DelSp=Yes 10, 19 -- MIME-Version: 1.0 10, 19 -- Content-Disposition: inline ==============================End of - Headers==============================
From medications-best4-at-windjammercable.net Thu Jul 10 22:16:56 2014 Return-Path: {medications-best4-at-windjammercable.net} Received: from mail4.windjammercable.net ([211.141.217.197]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6B3GoLO021511 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 10 Jul 2014 22:16:52 -0500 Message-Id: {201407110316.s6B3GoLO021511-at-ns.microscopy.com}
X-from: derrick.horne-at-botany.ubc.ca ()
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Title-Subject: [Filtered] Radioisotopes in the TEM (Ga-67)
Message: We've received a request for imaging Ga67-containing particles in the TEM. Our facility has very little experience with radioisotopes.
Ga67 is a gamma emitter, with a 1/2 life of 3.6 days. The Ga is supposedly bound within the matrix of the particles, but I do not have the details of the kind of material in which the Ga is embedded. The dose on a single grid works out to be around 2 nCi. We'd most likely have to image 1-2 grids.
What pitfalls and precautions should be considered when dealing with this kind of sample, if we choose to handle it? My main concerns are Ga migration, cross-contamination, and personnel safety.
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Email: mlibbee-at-lbl.gov Name: Marissa
Organization: NCEM, LBNL
Title-Subject: [Filtered] annealing nanoparticles on c-film grids
Message: Has anyone annealed nanoparticles on TEM c-film grids? If so, can you tell me the temp and time at which the carbon started to break down? And the specific grid you used for the experiment?
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I was wondering if any of you could help me out. We have recently installed a new SDD on our Jeol 1200 mk2 TEM it has a ASID with a SAP10 polepice and it is mounted on the high angle 70 degree port. We have a standard tungsten source in this one. The problem we have is we are getting a lot less stored counts with the new detector compared to the old SiLi detector, and we are picking up more noise and stray. We have rulled out hardward, software, and site but are now looking at geometry.
I was wondering if anyone out there who has the same TEM (with/without ASID) with EDS in the same port could let me know make, model, toa, crystal tilt and flange angle or any similar experiences it would be very useful. if you have a rough idea of counts at a given setup that would also be really helpful.
Thanks in advance John
==============================Original Headers============================== 4, 26 -- From john.mitchels-at-gmail.com Fri Jul 11 06:37:49 2014 4, 26 -- Received: from mail-vc0-f173.google.com (mail-vc0-f173.google.com [209.85.220.173]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6BBbmd7001855 4, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jul 2014 06:37:49 -0500 4, 26 -- Received: by mail-vc0-f173.google.com with SMTP id lf12so1740969vcb.18 4, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jul 2014 04:37:48 -0700 (PDT) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=20120113; 4, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 26 -- bh=mdWT+cYmzDjPhfCNzKvqHtCORhi0ves/t6f3JRB+wBI=; 4, 26 -- b=Sr3g/PEGrMFRA9eXFf7zjqyFL3mnV0xJa4v9SCbwz7DMqSn6IBjgoe5ZsCnL2sK3Cx 4, 26 -- kMaW4hjJbF4y6dyIsLs/wTC1UVF+Tnu6KNrb15M9F0v58jv36DpqFQqM3uLOtCd4J+9d 4, 26 -- Ps9DOEUbqWvQElSFNLHZUltaLWNVsxoVFMHZtvXbeMk6yA7XTaGzpYPhagEw9jASOc84 4, 26 -- XnfSnRJNBDIvcTB/5aoipFZz0RxT/Oe0tCeKhD4Oa0+z5UoB1S4hsxwQVav9RNbWbsbn 4, 26 -- OARQs8JVHaVU8GQ42i8AK1ZWN5757LCS6tmAc249EydEcY0zoQDsK1buQvm/JYih2bZj 4, 26 -- dZHA== 4, 26 -- MIME-Version: 1.0 4, 26 -- X-Received: by 10.52.182.163 with SMTP id ef3mr42689726vdc.22.1405078668509; 4, 26 -- Fri, 11 Jul 2014 04:37:48 -0700 (PDT) 4, 26 -- Received: by 10.220.6.136 with HTTP; Fri, 11 Jul 2014 04:37:48 -0700 (PDT) 4, 26 -- Date: Fri, 11 Jul 2014 12:37:48 +0100 4, 26 -- Message-ID: {CAHX7yTSxebXCMZrQ1QG7MxdFQMKQw5VdWWk2=qCT1dpUxevHdg-at-mail.gmail.com} 4, 26 -- Subject: JEOL 1200EXII TEM and EDX Question 4, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
From bimyekno-at-net.upcbroadband.cz Fri Jul 11 08:07:46 2014 Return-Path: {bimyekno-at-net.upcbroadband.cz} Received: from ip-94-112-182-15.net.upcbroadband.cz (ip-94-112-182-15.net.upcbroadband.cz [94.112.182.15]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6BD7iP0022091 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 11 Jul 2014 08:07:45 -0500 Message-ID: {486D3800.3637209-at-net.upcbroadband.cz}
Dear Marissa,
unfortunately I can't help with this your request anyway.
But in case you are also on the platform {ResearchGate} http://www.researchgate.net/
you should enter the Link/Address (= search phrase): https://www.researchgate.net/search.Search.html?query=Annealing%20nanoparticles&type=question an you'll get a lot of threads (Questions and answers) dealing with {annealing...} , some of them also of {nanoparticles}
Best wishes, good luck and have a nice and recreative weekend,
Wolfgang
Wolfgang MUSS PhD Member of MSA EM-Lab, Pathology SALK-LKH & PMU SALZBURG AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] } Gesendet: Freitag, 11. Juli 2014 13:20 } An: Muß Wolfgang } Betreff: [Microscopy] Annealing nanoparticles on c-film grids } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } X-from: mlibbee-at-lbl.gov () } This Question/Comment was submitted to the Microscopy Listserver using } the } WWW based Form at http://www.microscopy.com/MicroscopyListserver } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both mlibbee-at-lbl.gov as well as the Microscopy } Listserver } ----------------------------------------------------------------------- } ---- } Email: mlibbee-at-lbl.gov Name: Marissa } Organization: NCEM, LBNL } Title-Subject: annealing nanoparticles on c-film } grids } Message: } } Has anyone annealed nanoparticles on TEM c-film grids? If so, can you } tell me the temp and time at which the carbon started to break } down? } And the specific grid you used for the experiment? } } Login Host: 131.243.3.211 } Listserver Email Form V - 20120416 } ----------------------------------------------------------------------- } ---- } -- } =============================================== } Do not reply to this messages it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } Microscopy-at-Microscopy.com } ============================================= } ==============================Original } Headers============================== } 12, 40 -- From nestor.zaluzec-at-gmail.com Fri Jul 11 06:15:41 2014 } 12, 40 -- Received: from mail-pd0-f173.google.com (mail-pd0- } f173.google.com [209.85.192.173]) } 12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s6BBFfDE029375 } 12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2014 06:15:41 } -0500 } 12, 40 -- Received: by mail-pd0-f173.google.com with SMTP id } r10so1238677pdi.4 } 12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2014 } 04:15:40 -0700 (PDT) } 12, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 40 -- d=gmail.com; s=20120113; } 12, 40 -- h=sender:from:message-id:date:reply-to:user- } agent:mime-version:to } 12, 40 -- :subject:references:in-reply-to:content-type } 12, 40 -- :content-transfer-encoding; } 12, 40 -- bh=tvkIRojlkt04mPs6yZ0U8HcTwMSq42u3VE9ZjhYFEKI=; } 12, 40 -- } b=SAqfjMNEPDwVOWAVGeb0zhseewx49yZqoLEkFonjLK2l5//3F2l8M99UuZEtfVVoMp } 12, 40 -- } JaFFxFn7/NiAPZrbNMNyWYZYI3M8l3c0HDfhEG9YD/tGJMe2TyUl2vWhMch9wqOqBIk5 } 12, 40 -- } WlEMh0BexwTIY6UiBEXxANPc6cnwMZgnNGdD44TKTBGjYJUGkU2y3ipyqpjEBL86Lf+u } 12, 40 -- } dOllRgpEupvqxcqLb4J72Kh6+7hyonudMJ1CBPZWXZXmagCY9Ug3ByT9Dfs7MOqyW9rI } 12, 40 -- } k8QC14sBHdVlpUfjUGfN9UL1MH9R58D+8kYRlKlobp74/tjrLb88l5Ew3kqoenTjE0qk } 12, 40 -- r+HQ== } 12, 40 -- X-Received: by 10.66.155.227 with SMTP id } vz3mr52703005pab.26.1405077340829; } 12, 40 -- Fri, 11 Jul 2014 04:15:40 -0700 (PDT) } 12, 40 -- Received: from Nestors-MacBook-Pro-2014.local } ([61.16.155.25]) } 12, 40 -- by mx.google.com with ESMTPSA id } kq10sm1985940pbc.90.2014.07.11.04.15.38 } 12, 40 -- for {microscopy-at-microscopy.com} } 12, 40 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA } bits=128/128); } 12, 40 -- Fri, 11 Jul 2014 04:15:39 -0700 (PDT) } 12, 40 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} } 12, 40 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} } 12, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 12, 40 -- Message-ID: {53BFC75A.6030501-at-microscopy.com} } 12, 40 -- Date: Fri, 11 Jul 2014 16:45:38 +0530 } 12, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 12, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; } rv:24.0) Gecko/20100101 Thunderbird/24.6.0 } 12, 40 -- MIME-Version: 1.0 } 12, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 40 -- Subject: viaWWW:annealing nanoparticles on c-film grids } 12, 40 -- References: {201407102145.s6ALjvJt030759-at-ns.microscopy.com} } 12, 40 -- In-Reply-To: {201407102145.s6ALjvJt030759-at-ns.microscopy.com} } 12, 40 -- X-Forwarded-Message-Id: } {201407102145.s6ALjvJt030759-at-ns.microscopy.com} } 12, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 40 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 11, 39 -- From W.Muss-at-salk.at Fri Jul 11 11:05:53 2014 11, 39 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 11, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6BG5qGL024520 11, 39 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2014 11:05:53 -0500 11, 39 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 11, 39 -- by hermes.salk.at (Postfix) with ESMTP id B4EB5646B70 11, 39 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2014 18:05:51 +0200 (CEST) 11, 39 -- X-Scanned-By: SALK-Content-Filter 11, 39 -- Received: from hermes.salk.at ([127.0.0.1]) 11, 39 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 11, 39 -- with ESMTP id 7KNXDCIExijT for {microscopy-at-microscopy.com} ; 11, 39 -- Fri, 11 Jul 2014 18:05:51 +0200 (CEST) 11, 39 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 11, 39 -- by hermes.salk.at (Postfix) with ESMTP id 771CD646B2D 11, 39 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2014 18:05:51 +0200 (CEST) 11, 39 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 11, 39 -- by N1EX198.lks.local with ESMTP; 11 Jul 2014 18:15:33 +0200 11, 39 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 11, 39 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 11, 39 -- 14.02.0387.000; Fri, 11 Jul 2014 18:05:51 +0200 11, 39 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 11, 39 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 11, 39 -- CC: "'mlibbee-at-lbl.gov'" {mlibbee-at-lbl.gov} 11, 39 -- Subject: Re: [Microscopy] Annealing nanoparticles on c-film grids 11, 39 -- Thread-Topic: [Microscopy] Annealing nanoparticles on c-film grids 11, 39 -- Thread-Index: AQHPnSH7rEH9W9oyDEuHjOz1ems3vA== 11, 39 -- Date: Fri, 11 Jul 2014 16:05:50 +0000 11, 39 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D3D7B5B-at-N2DT131.lksdom21.lks.local} 11, 39 -- References: {201407111119.s6BBJvMt004674-at-ns.microscopy.com} 11, 39 -- In-Reply-To: {201407111119.s6BBJvMt004674-at-ns.microscopy.com} 11, 39 -- Accept-Language: de-DE, en-US 11, 39 -- Content-Language: de-DE 11, 39 -- X-MS-Has-Attach: 11, 39 -- X-MS-TNEF-Correlator: 11, 39 -- x-originating-ip: [192.168.42.2] 11, 39 -- Content-Type: text/plain; charset="iso-8859-1" 11, 39 -- MIME-Version: 1.0 11, 39 -- Content-Transfer-Encoding: 8bit 11, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6BG5qGL024520 ==============================End of - Headers==============================
Our first annual student poster and speaker competition will be held at the Frederick Seitz Materials Research Laboratory this coming November at our third annual Biological Structures workshop.
This is a conference covering biological imaging, or a combination of biological and materials imaging.
November 12-13th Winners will receive $100 check and their image and names in local papers and on our web site.
In order to give Researchers time to allow their students to prepare, we already have all the rules, regulations and registration up and ready for participants at our online site.
Your registration is also ready for the workshop, and includes the ability to help contribute to the contest prize ( email Lou Ann at lamiller-at-illinois.edu for details and if such things are yet filled) or help be a pizza sponsor for the second day ( able to do at registration).
Those vendors offering to help with the prize will be limited to $100 ( state & University regulations), will be able to name the prize after your company, and will be included in judging and images that go to the local papers and online.
We are still formulating our tutorial speaker program, that will be posted as soon as we have it. Vendors, there is an opportunity to give talks, they must be tutorial in nature. If time permits 45 min, but be prepared for a 30 min slot. A third day may be set up where all can come for free if the numbers of presentations permit.
Our facilities has a PC, slots for USB, laptop connection( Mac & PC), laser pointers and lapel microphone. We have poster size regulations in the poster competition rules, speaker rules are also online.
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230
==============================Original Headers============================== 21, 37 -- From lamiller-at-illinois.edu Fri Jul 11 11:07:49 2014 21, 37 -- Received: from pps04.cites.illinois.edu (pps04.cites.illinois.edu [192.17.82.101]) 21, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6BG7n7t026172 21, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jul 2014 11:07:49 -0500 21, 37 -- Received: from citesht2.cites.illinois.edu (citesht2.cites.illinois.edu [128.174.34.207]) 21, 37 -- by pps04.cites.illinois.edu (8.14.5/8.14.5) with ESMTP id s6BG6sBh013192 21, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 21, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jul 2014 11:07:48 -0500 21, 37 -- Received: from CITESMBX2.ad.uillinois.edu ([169.254.2.77]) by 21, 37 -- CITESHT2.ad.uillinois.edu ([128.174.34.207]) with mapi id 14.03.0181.006; 21, 37 -- Fri, 11 Jul 2014 11:07:35 -0500 21, 37 -- From: "Miller, Lou A" {lamiller-at-illinois.edu} 21, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 21, 37 -- Subject: MRL UIUC: Opening Up Biological Microscopy Student Poster and 21, 37 -- Speaker Competitions and a note for Vendors 21, 37 -- Thread-Topic: MRL UIUC: Opening Up Biological Microscopy Student Poster and 21, 37 -- Speaker Competitions and a note for Vendors 21, 37 -- Thread-Index: AQHPnSI6P/0GjJWrw0W3NwFrA1HMWg== 21, 37 -- Date: Fri, 11 Jul 2014 16:07:35 +0000 21, 37 -- Message-ID: {D1764F8C-27FD-484C-B1FC-6703AF8AB525-at-illinois.edu} 21, 37 -- Accept-Language: en-US 21, 37 -- Content-Language: en-US 21, 37 -- X-MS-Has-Attach: 21, 37 -- X-MS-TNEF-Correlator: 21, 37 -- x-originating-ip: [128.174.34.199] 21, 37 -- Content-Type: text/plain; charset="us-ascii" 21, 37 -- Content-ID: {D759849F5BA06A45B21AF44611326BB4-at-mx.uillinois.edu} 21, 37 -- MIME-Version: 1.0 21, 37 -- X-Spam-Score: 0 21, 37 -- X-Spam-Details: rule=cautious_plus_nq_notspam policy=cautious_plus_nq score=0 spamscore=0 21, 37 -- suspectscore=0 phishscore=0 adultscore=0 bulkscore=1 classifier=spam 21, 37 -- adjust=0 reason=mlx scancount=1 engine=7.0.1-1402240000 21, 37 -- definitions=main-1407110194 21, 37 -- X-Spam-OrigSender: lamiller-at-illinois.edu 21, 37 -- X-Spam-Bar: 21, 37 -- Content-Transfer-Encoding: 8bit 21, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6BG7n7t026172 ==============================End of - Headers==============================
From drugs-cheapest1-at-marveldirectory.com Fri Jul 11 13:27:13 2014 Return-Path: {drugs-cheapest1-at-marveldirectory.com} Received: from mail4.marveldirectory.com (95.59.124.192.megaline.telecom.kz [95.59.124.192] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6BIR8FI031968 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 11 Jul 2014 13:27:09 -0500 Message-Id: {201407111827.s6BIR8FI031968-at-ns.microscopy.com} X-AntiVirus: Checked by Dr.Web [version: 8.0.0.11152, engine: 8.0.1.11280, virus records: 3407154, updated: 6.12.2012]
Dear Marissa,
Carbon films are stable in the TEM vacuum up to 1000°C and even far beyond. But the choice of the film supporting grid is critical.
I used with success gold 200 mesh grids with thin carbon films during my PhD work on the melting temperature of gold nanocrystals (Buffat, Phys.Rev. A13 (1976) 2287).
A priori molybdenum grids should had allowed high temperatures with good mechanical stability. But it rapidly appeared that the C-film reacts with Mo to form Mo carbide and cracks (see for instance Leroy et al, J.Appl.Phys 99 (2006) 063704 about Mo-C reactivity). Copper grids were excluded because of the risk of alloying with gold. Eventually gold grids were the best compromise though the largest Au crystals did melt only a few degrees before the supporting grid itself.
The vacuum in the electron diffraction camera (a TEM without magnifiying section) was 2 10-6 mbar. A slow heating cycle took about 15 minutes and I didn't observed any significant damage to the film when the sample was transferred to a TEM for crystal size observation.
More experiments were performed with heating in a TEM (Philips EM430) though with less temperature measurement accuracy, but still no significant film damage due to heating.
However it appeared that the carbon film may destroys under strong electron beam during HRTEM observation. At that time we still used photographic plan films and we noticed a clear correlation between the film sputtering speed and the time between loading films in the camera and HRTEM observation. This shows that despite the presence of a differential pressure aperture between the plate camera and the TEM column itself, water (or methane?) molecules were able to fly straight toward the sample and enhance sputtering (the mean free path of molecules is about meter(s) in this vacuum range). Film stability under strong electron beams depends more on the vacuum quality and residual atmosphere composition than temperature.
From medications-reasonable7-at-thrane.com Sat Jul 12 18:18:13 2014 Return-Path: {medications-reasonable7-at-thrane.com} Received: from mail2.thrane.com ([31.135.56.0]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6CNIA9l015157 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 12 Jul 2014 18:18:11 -0500 Message-Id: {201407122318.s6CNIA9l015157-at-ns.microscopy.com}
Dear all,
I am studying cellulose aerogel made from Nanocellulose Fibres (10-30nm diameter and several micron in length).
MATERIAL: The material show highly porosity (up to 90% air) and a very intricate structure with meso and nano pores.
SAMPLE: Samples are produced via supercritical CO2 drying after filtration and solvent exchange. The resulting sample is a ""paper-like"" fluffy material, 3 cm in diameter and a thickness of 300 micron.
SEM: To study the microstructure I tried to embed a small piece of aerogel in araldite. The cross-section surface of the sample was exposed with a diamond microtome and sputtered with 20nm of gold.
In the obtained images is difficult to distinguish between the araldite matrix and the sample (a small change in contrast at low magnification). Worst is that on the surface I don't see any micro-structure. Only a light grey smooth surface.
QUESTION: - Is it possible in your opinion to obtain a nice cross section image of my aerogel? - I am thinking to change the embedding material with a more stiff epoxy. It could help? - Do you have any others ideas?
Many thanks in advance, Ferruccio
PS: This is my first message. Hope I did everything correct.
==============================Original Headers============================== 11, 41 -- From Ferruccio.Bolla-at-empa.ch Mon Jul 14 01:18:38 2014 11, 41 -- Received: from barracuda4.eawag.ch (barracuda4.eawag.ch [152.88.7.197]) 11, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6E6Ic3m013174 11, 41 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 01:18:38 -0500 11, 41 -- X-ASG-Debug-ID: 1405318687-0814c273851bb440001-JFxeax 11, 41 -- Received: from ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) by barracuda4.eawag.ch with ESMTP id m4myH1y77uhCEkyB for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 08:18:37 +0200 (CEST) 11, 41 -- X-Barracuda-Envelope-From: Ferruccio.Bolla-at-empa.ch 11, 41 -- X-Barracuda-Apparent-Source-IP: 152.88.45.32 11, 41 -- Received: from EE-MBX2.ee.emp-eaw.ch ([169.254.2.218]) by 11, 41 -- ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) with mapi id 14.03.0181.006; Mon, 14 11, 41 -- Jul 2014 08:18:06 +0200 11, 41 -- From: "Bolla, Ferruccio" {Ferruccio.Bolla-at-empa.ch} 11, 41 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 11, 41 -- Subject: SEM - Cellulose sample preparation - embedding 11, 41 -- Thread-Topic: SEM - Cellulose sample preparation - embedding 11, 41 -- X-ASG-Orig-Subj: SEM - Cellulose sample preparation - embedding 11, 41 -- Thread-Index: Ac+fKyau6wTbxpSIStmaw429JY0agA== 11, 41 -- Date: Mon, 14 Jul 2014 06:18:06 +0000 11, 41 -- Message-ID: {6E81EA4991422D448926980E794FEB5EE2315A-at-ee-mbx2.ee.emp-eaw.ch} 11, 41 -- Accept-Language: de-CH, en-US 11, 41 -- Content-Language: en-US 11, 41 -- X-MS-Has-Attach: 11, 41 -- X-MS-TNEF-Correlator: 11, 41 -- x-originating-ip: [152.88.94.96] 11, 41 -- Content-Type: text/plain; charset="us-ascii" 11, 41 -- MIME-Version: 1.0 11, 41 -- X-Barracuda-Connect: UNKNOWN[152.88.45.32] 11, 41 -- X-Barracuda-Start-Time: 1405318717 11, 41 -- X-Barracuda-URL: http://152.88.7.197:8000/cgi-mod/mark.cgi 11, 41 -- X-Virus-Scanned: by bsmtpd at eawag.ch 11, 41 -- X-Barracuda-BRTS-Status: 1 11, 41 -- X-Barracuda-Spam-Score: 2.80 11, 41 -- X-Barracuda-Spam-Status: No, SCORE=2.80 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=WEIRD_QUOTING, WEIRD_QUOTING_2 11, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.7498 11, 41 -- Rule breakdown below 11, 41 -- pts rule name description 11, 41 -- ---- ---------------------- -------------------------------------------------- 11, 41 -- 0.00 WEIRD_QUOTING BODY: Weird repeated double-quotation marks 11, 41 -- 2.80 WEIRD_QUOTING_2 Weird repeated double-quotation marks 11, 41 -- Content-Transfer-Encoding: 8bit 11, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6E6Ic3m013174 ==============================End of - Headers==============================
Hi, Can you explain why you want to embed your sample? Particularly if you can coat with something finer than gold (platinum, carbon) and if you have access to some low voltage/charge minimizing features of the recent generation of SEMs, it should work. Just a thought.
Tobias Baskin
On 7/14/14, 2:19 AM, Ferruccio.Bolla-at-empa.ch wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } I am studying cellulose aerogel made from Nanocellulose Fibres (10-30nm diameter and several micron in length). } } MATERIAL: } The material show highly porosity (up to 90% air) and a very intricate structure with meso and nano pores. } } SAMPLE: } Samples are produced via supercritical CO2 drying after filtration and solvent exchange. The resulting sample is a ""paper-like"" fluffy material, 3 cm in diameter and a thickness of 300 micron. } } SEM: } To study the microstructure I tried to embed a small piece of aerogel in araldite. The cross-section surface of the sample was exposed with a diamond microtome and sputtered with 20nm of gold. } } In the obtained images is difficult to distinguish between the araldite matrix and the sample (a small change in contrast at low magnification). Worst is that on the surface I don't see any micro-structure. Only a light grey smooth surface. } } QUESTION: } - Is it possible in your opinion to obtain a nice cross section image of my aerogel? } - I am thinking to change the embedding material with a more stiff epoxy. It could help? } - Do you have any others ideas? } } Many thanks in advance, } Ferruccio } } } PS: This is my first message. Hope I did everything correct. } } } ==============================Original Headers============================== } 11, 41 -- From Ferruccio.Bolla-at-empa.ch Mon Jul 14 01:18:38 2014 } 11, 41 -- Received: from barracuda4.eawag.ch (barracuda4.eawag.ch [152.88.7.197]) } 11, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6E6Ic3m013174 } 11, 41 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 01:18:38 -0500 } 11, 41 -- X-ASG-Debug-ID: 1405318687-0814c273851bb440001-JFxeax } 11, 41 -- Received: from ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) by barracuda4.eawag.ch with ESMTP id m4myH1y77uhCEkyB for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 08:18:37 +0200 (CEST) } 11, 41 -- X-Barracuda-Envelope-From: Ferruccio.Bolla-at-empa.ch } 11, 41 -- X-Barracuda-Apparent-Source-IP: 152.88.45.32 } 11, 41 -- Received: from EE-MBX2.ee.emp-eaw.ch ([169.254.2.218]) by } 11, 41 -- ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) with mapi id 14.03.0181.006; Mon, 14 } 11, 41 -- Jul 2014 08:18:06 +0200 } 11, 41 -- From: "Bolla, Ferruccio" {Ferruccio.Bolla-at-empa.ch} } 11, 41 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 11, 41 -- Subject: SEM - Cellulose sample preparation - embedding } 11, 41 -- Thread-Topic: SEM - Cellulose sample preparation - embedding } 11, 41 -- X-ASG-Orig-Subj: SEM - Cellulose sample preparation - embedding } 11, 41 -- Thread-Index: Ac+fKyau6wTbxpSIStmaw429JY0agA== } 11, 41 -- Date: Mon, 14 Jul 2014 06:18:06 +0000 } 11, 41 -- Message-ID: {6E81EA4991422D448926980E794FEB5EE2315A-at-ee-mbx2.ee.emp-eaw.ch} } 11, 41 -- Accept-Language: de-CH, en-US } 11, 41 -- Content-Language: en-US } 11, 41 -- X-MS-Has-Attach: } 11, 41 -- X-MS-TNEF-Correlator: } 11, 41 -- x-originating-ip: [152.88.94.96] } 11, 41 -- Content-Type: text/plain; charset="us-ascii" } 11, 41 -- MIME-Version: 1.0 } 11, 41 -- X-Barracuda-Connect: UNKNOWN[152.88.45.32] } 11, 41 -- X-Barracuda-Start-Time: 1405318717 } 11, 41 -- X-Barracuda-URL: http://152.88.7.197:8000/cgi-mod/mark.cgi } 11, 41 -- X-Virus-Scanned: by bsmtpd at eawag.ch } 11, 41 -- X-Barracuda-BRTS-Status: 1 } 11, 41 -- X-Barracuda-Spam-Score: 2.80 } 11, 41 -- X-Barracuda-Spam-Status: No, SCORE=2.80 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=WEIRD_QUOTING, WEIRD_QUOTING_2 } 11, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.7498 } 11, 41 -- Rule breakdown below } 11, 41 -- pts rule name description } 11, 41 -- ---- ---------------------- -------------------------------------------------- } 11, 41 -- 0.00 WEIRD_QUOTING BODY: Weird repeated double-quotation marks } 11, 41 -- 2.80 WEIRD_QUOTING_2 Weird repeated double-quotation marks } 11, 41 -- Content-Transfer-Encoding: 8bit } 11, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6E6Ic3m013174 } ==============================End of - Headers============================== }
-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 USA / /___ / \ \___/ \_____ 413 - 545 - 1533 www.bio.umass.edu/biology/baskin
Why embed the aerogel? I've done aerogel in the SEM, but never with embedding. Coat with something other than plain gold - Au/Pd, Pt, or something - and use low kV. "Low kV" depends on what SEM you have, but no more than 5 kV. If you can go down to 1 or 1.5 kV, that would be better. Possibly lower. If you can find the kV at which the charges on the sample balance, you may not need coating at all. Also, you can submerge the aerogel in liquid nitrogen and carefully snap it to expose the interior. I'm assuming the fibers have glass transition above the LN2 temperature, but I'm sure it does.
If you have to embed the nanocellulose fibers in Araldite, you can stain the Araldite with RuO4: place the sectioned blocks in a sealed dish in a fume hood with a crystal or two of RuO4, and let the vapors stain the resin. The nanocellulose should remain unstained. Or: sputter coat the aerogel with gold or any heavy metal, but use shorter coating runs, and turn over the block of aerogel between coats, so the metal gets equal access to the aerogel from all sides. Then embed. The fibers will be the bits that are surronded by the heavy metal, which will show up in the SEM. Mind, this last is only good for sections of the fibers, the surface will be obscured.
Phil
} Dear all, } } I am studying cellulose aerogel made from Nanocellulose Fibres (10-30nm diameter and several micron in length). } } MATERIAL: } The material show highly porosity (up to 90% air) and a very intricate structure with meso and nano pores. } } SAMPLE: } Samples are produced via supercritical CO2 drying after filtration and solvent exchange. The resulting sample is a ""paper-like"" fluffy material, 3 cm in diameter and a thickness of 300 micron. } } SEM: } To study the microstructure I tried to embed a small piece of aerogel in araldite. The cross-section surface of the sample was exposed with a diamond microtome and sputtered with 20nm of gold. } } In the obtained images is difficult to distinguish between the araldite matrix and the sample (a small change in contrast at low magnification). Worst is that on the surface I don't see any micro-structure. Only a light grey smooth surface. } } QUESTION: } - Is it possible in your opinion to obtain a nice cross section image of my aerogel? } - I am thinking to change the embedding material with a more stiff epoxy. It could help? } - Do you have any others ideas? } } Many thanks in advance, } Ferruccio } PS: This is my first message. Hope I did everything correct.
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 29 -- From oshel1pe-at-cmich.edu Mon Jul 14 08:23:21 2014 7, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6EDNLaD024816 7, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 08:23:21 -0500 7, 29 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 7, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s6EDNKZ1026689 7, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 09:23:20 -0400 7, 29 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 7, 29 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 7, 29 -- 14.3.181.6; Mon, 14 Jul 2014 09:23:20 -0400 7, 29 -- Message-ID: {53C3D9C8.5050303-at-cmich.edu} 7, 29 -- Date: Mon, 14 Jul 2014 09:23:20 -0400 7, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 29 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 29 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 29 -- MIME-Version: 1.0 7, 29 -- To: micro {Microscopy-at-microscopy.com} 7, 29 -- Subject: Re: [Microscopy] SEM - Cellulose sample preparation - embedding 7, 29 -- References: {201407140633.s6E6XI6k030464-at-ns.microscopy.com} 7, 29 -- In-Reply-To: {201407140633.s6E6XI6k030464-at-ns.microscopy.com} 7, 29 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 7, 29 -- Content-Transfer-Encoding: 7bit 7, 29 -- X-Originating-IP: [141.209.160.122] 7, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 29 -- X-Spam-Score: -0.50 () [Hold at 6.00] WEIRD_QUOTING,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 7, 29 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 7, 29 -- X-CanItPRO-Stream: default 7, 29 -- X-Canit-Stats-ID: 02MqdnkKU - 289788c5d5e4 - 20140714 7, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Hi Ferruccio If you don't manage to manually open the aerogel successfully, you can try to embed it paraffine and get some thick sections, which then you treat with a warm (} 70C) solven compatible with your staff to get rid of the wax and then coat and view in SEM? best yorgos
----- Original Message ----- X-from: "Bolla, Ferruccio" {Ferruccio.Bolla-at-empa.ch} To: "yorgos nikas" {eikonika-at-otenet.gr} Sent: Monday, July 14, 2014 12:46 PM
Hi Ferruccio, it might be the same problem like cutting polystyrene (styropor). I succeeded only with a piece of polystyrene submerged in liquid nitrogen and then using a NEW razorblade cooled at LN2 temp to cut the polystyrene. The cells and walls looked relatively unharmed in the SEM afterwards. See some images of it here: http://www.electronmicroscopy.info/shop_materials.htm image number 27 to 31...
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 14.07.14 08:24, schrieb Ferruccio.Bolla-at-empa.ch: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } I am studying cellulose aerogel made from Nanocellulose Fibres (10-30nm diameter and several micron in length). } } MATERIAL: } The material show highly porosity (up to 90% air) and a very intricate structure with meso and nano pores. } } SAMPLE: } Samples are produced via supercritical CO2 drying after filtration and solvent exchange. The resulting sample is a ""paper-like"" fluffy material, 3 cm in diameter and a thickness of 300 micron. } } SEM: } To study the microstructure I tried to embed a small piece of aerogel in araldite. The cross-section surface of the sample was exposed with a diamond microtome and sputtered with 20nm of gold. } } In the obtained images is difficult to distinguish between the araldite matrix and the sample (a small change in contrast at low magnification). Worst is that on the surface I don't see any micro-structure. Only a light grey smooth surface. } } QUESTION: } - Is it possible in your opinion to obtain a nice cross section image of my aerogel? } - I am thinking to change the embedding material with a more stiff epoxy. It could help? } - Do you have any others ideas? } } Many thanks in advance, } Ferruccio } } } PS: This is my first message. Hope I did everything correct. } } } ==============================Original Headers============================== } 11, 41 -- From Ferruccio.Bolla-at-empa.ch Mon Jul 14 01:18:38 2014 } 11, 41 -- Received: from barracuda4.eawag.ch (barracuda4.eawag.ch [152.88.7.197]) } 11, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6E6Ic3m013174 } 11, 41 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 01:18:38 -0500 } 11, 41 -- X-ASG-Debug-ID: 1405318687-0814c273851bb440001-JFxeax } 11, 41 -- Received: from ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) by barracuda4.eawag.ch with ESMTP id m4myH1y77uhCEkyB for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 08:18:37 +0200 (CEST) } 11, 41 -- X-Barracuda-Envelope-From: Ferruccio.Bolla-at-empa.ch } 11, 41 -- X-Barracuda-Apparent-Source-IP: 152.88.45.32 } 11, 41 -- Received: from EE-MBX2.ee.emp-eaw.ch ([169.254.2.218]) by } 11, 41 -- ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) with mapi id 14.03.0181.006; Mon, 14 } 11, 41 -- Jul 2014 08:18:06 +0200 } 11, 41 -- From: "Bolla, Ferruccio" {Ferruccio.Bolla-at-empa.ch} } 11, 41 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 11, 41 -- Subject: SEM - Cellulose sample preparation - embedding } 11, 41 -- Thread-Topic: SEM - Cellulose sample preparation - embedding } 11, 41 -- X-ASG-Orig-Subj: SEM - Cellulose sample preparation - embedding } 11, 41 -- Thread-Index: Ac+fKyau6wTbxpSIStmaw429JY0agA== } 11, 41 -- Date: Mon, 14 Jul 2014 06:18:06 +0000 } 11, 41 -- Message-ID: {6E81EA4991422D448926980E794FEB5EE2315A-at-ee-mbx2.ee.emp-eaw.ch} } 11, 41 -- Accept-Language: de-CH, en-US } 11, 41 -- Content-Language: en-US } 11, 41 -- X-MS-Has-Attach: } 11, 41 -- X-MS-TNEF-Correlator: } 11, 41 -- x-originating-ip: [152.88.94.96] } 11, 41 -- Content-Type: text/plain; charset="us-ascii" } 11, 41 -- MIME-Version: 1.0 } 11, 41 -- X-Barracuda-Connect: UNKNOWN[152.88.45.32] } 11, 41 -- X-Barracuda-Start-Time: 1405318717 } 11, 41 -- X-Barracuda-URL: http://152.88.7.197:8000/cgi-mod/mark.cgi } 11, 41 -- X-Virus-Scanned: by bsmtpd at eawag.ch } 11, 41 -- X-Barracuda-BRTS-Status: 1 } 11, 41 -- X-Barracuda-Spam-Score: 2.80 } 11, 41 -- X-Barracuda-Spam-Status: No, SCORE=2.80 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=WEIRD_QUOTING, WEIRD_QUOTING_2 } 11, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.7498 } 11, 41 -- Rule breakdown below } 11, 41 -- pts rule name description } 11, 41 -- ---- ---------------------- -------------------------------------------------- } 11, 41 -- 0.00 WEIRD_QUOTING BODY: Weird repeated double-quotation marks } 11, 41 -- 2.80 WEIRD_QUOTING_2 Weird repeated double-quotation marks } 11, 41 -- Content-Transfer-Encoding: 8bit } 11, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6E6Ic3m013174 } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 23 -- From stefan.diller-at-t-online.de Mon Jul 14 09:32:20 2014 7, 23 -- Received: from mailout11.t-online.de (mailout11.t-online.de [194.25.134.85]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6EEWJpc031675 7, 23 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 09:32:19 -0500 7, 23 -- Received: from fwd34.aul.t-online.de (fwd34.aul.t-online.de [172.20.26.145]) 7, 23 -- by mailout11.t-online.de (Postfix) with SMTP id 9EED75C7923; 7, 23 -- Mon, 14 Jul 2014 16:32:18 +0200 (CEST) 7, 23 -- Received: from mac-pro.local (V+vFv0ZAohFZi67itc6NHoXmsUXiidB3Yvk47vN1xH0i7PDkh62wA5aRY9UN-8LgHa-at-[91.58.170.232]) by fwd34.t-online.de 7, 23 -- with (TLSv1:ECDHE-RSA-AES256-SHA encrypted) 7, 23 -- esmtp id 1X6hIW-2zauZs0; Mon, 14 Jul 2014 16:32:08 +0200 7, 23 -- Message-ID: {53C3E9E8.50403-at-t-online.de} 7, 23 -- Date: Mon, 14 Jul 2014 16:32:08 +0200 7, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 7, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 7, 23 -- MIME-Version: 1.0 7, 23 -- To: Ferruccio.Bolla-at-empa.ch, microscopy-at-microscopy.com 7, 23 -- Subject: Re: [Microscopy] SEM - Cellulose sample preparation - embedding 7, 23 -- References: {201407140624.s6E6ObO5018657-at-ns.microscopy.com} 7, 23 -- In-Reply-To: {201407140624.s6E6ObO5018657-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- X-ID: V+vFv0ZAohFZi67itc6NHoXmsUXiidB3Yvk47vN1xH0i7PDkh62wA5aRY9UN-8LgHa 7, 23 -- X-TOI-MSGID: adf32f96-571a-4435-adb6-72f9c1b8879f ==============================End of - Headers==============================
Dear All, recently, i am trying to help a user to section her sample embedded in Epo-Fix epoxy. As i am not very experience with ultramicrotome, I decide to section the Epo-Fix epoxy without any sample in it with a glass knife as a start. Unfortunately , i am unable to section it to a thickness of about 100nm. What i observed is that the glass knife is only able to section at alternating interval. I learn that this can be due to either the epoxy is quite soft of the glass knife is not sharp enough (or the block face might still be too big?). The student told me that that glass transition of the epoxy is above room temperature so there shouldn't be a need to adopt cryo-ultramicrotomy approach. I also attempt to trim the block face to a small size e.g to about 1mm (or slightly less) but still the same alternate sectioning observation is made. I have tried sectioning with biological sample embedded in aradite before and there is no problem achieving thickness of 100nm or less.
My glass knife cutting angle is at 6 degree.
May i ask if i have missed out anything? Is Epo-fix suitable to be sectioned with a glass knife to achieve a thickness of 100nm? On my second question, does the Tg of the sample determines if one should use a room-temperature ultramicrotome or a cryo-ultramicrotome?
Cheers, Yee Yan, Tay FACTS Lab Nanyang Technological University
==============================Original Headers============================== 7, 34 -- From rongchigram79-at-yahoo.com.sg Mon Jul 14 10:50:10 2014 7, 34 -- Received: from nm25-vm5.bullet.mail.sg3.yahoo.com (nm25-vm5.bullet.mail.sg3.yahoo.com [106.10.151.100]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6EFo8wA019577 7, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 10:50:09 -0500 7, 34 -- Received: from [106.10.166.115] by nm25.bullet.mail.sg3.yahoo.com with NNFMP; 14 Jul 2014 15:50:08 -0000 7, 34 -- Received: from [106.10.150.24] by tm4.bullet.mail.sg3.yahoo.com with NNFMP; 14 Jul 2014 15:50:08 -0000 7, 34 -- Received: from [127.0.0.1] by omp1025.mail.sg3.yahoo.com with NNFMP; 14 Jul 2014 15:50:08 -0000 7, 34 -- X-Yahoo-Newman-Property: ymail-3 7, 34 -- X-Yahoo-Newman-Id: 122254.43108.bm-at-omp1025.mail.sg3.yahoo.com 7, 34 -- Received: (qmail 14667 invoked by uid 60001); 14 Jul 2014 15:50:08 -0000 7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1405353008; bh=g3COM9sUeNA3at/5HiMHgtmYaexuRdFVSyKntOWTBJs=; h=Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=r3wLGOTt5CIg2ptmSZBF1XnA1q4TEd90lpwfgU1IFHa402cPCxmOE4mkkoFoXvhHqAMemrEDtYoxQVjrDl3JXG8gHpdtXxrkcbO4luQ4qBje1+x8b14dEh9diLKUy81BmZ3344qHzRLALVkI1bEa2zHe50rO6QPkab54MWuUWyE= 7, 34 -- X-YMail-OSG: GgdEbuUVM1ly9bGlDonM9KyRC7.H_IWyMUAedj2UJ4t0Kpj 7, 34 -- IEgWHXNvGa5pRFvZbEWTHgv0r.gCioH10CKIBg7fVDNH5naBPvgf9SaAUAvU 7, 34 -- tjF47nItfTlbdu4UKRne3VVQnauWIF4MmABumSMod.MM5unV8bJ99o9FZYif 7, 34 -- cfIpCgsE9gRjEWNfxbIJVlu_wx7SiT4D..pZvZflFp9yTy420GYnFioDLuYV 7, 34 -- o25e0fw7Vrw3J01e0C_cSPOhX1BjL_cHq1FVkOqtDlE8lsQ_s69xfcycf.fr 7, 34 -- cfMipV.ghsYpG1DsjViuauaJHExbXRW1BusyYu1LlvnENpzVdE5jEdknACzy 7, 34 -- YIuqgAjnstO3e749jA0t2qHTbDqQcejWN8LHAgQt7NXT6CJkiGMSyW1Mlpjy 7, 34 -- W43Rex7nNleaG0wEYMwquCgEfj9cca0c2OaIJIw7My5nawWOhez6dynPsID2 7, 34 -- yAOlbJBCpOcnelE1.YTy.CMv71OIREc1LoguK5pNtRbSCmHbu1bh4i2YqmfI 7, 34 -- w1C3ONg-- 7, 34 -- Received: from [121.6.130.175] by web190102.mail.sg3.yahoo.com via HTTP; Mon, 14 Jul 2014 23:50:07 SGT 7, 34 -- X-Rocket-MIMEInfo: 002.001,RGVhciBBbGwsIHJlY2VudGx5LCBpIGFtIHRyeWluZyB0byBoZWxwIGEgdXNlciB0byBzZWN0aW9uIGhlciBzYW1wbGUgZW1iZWRkZWQgaW4gRXBvLUZpeCBlcG94eS4gQXMgaSBhbSBub3QgdmVyeSBleHBlcmllbmNlIHdpdGggdWx0cmFtaWNyb3RvbWUsIEkgZGVjaWRlIHRvIHNlY3Rpb24gdGhlIEVwby1GaXggZXBveHkgd2l0aG91dCBhbnkgc2FtcGxlIGluIGl0IHdpdGggYSBnbGFzcyBrbmlmZSBhcyBhIHN0YXJ0LiBVbmZvcnR1bmF0ZWx5ICwgaSBhbSB1bmFibGUgdG8gc2VjdGlvbiBpdCB0byBhIHRoaWMBMAEBAQE- 7, 34 -- X-Mailer: YahooMailWebService/0.8.194.680 7, 34 -- Message-ID: {1405353007.91095.YahooMailNeo-at-web190102.mail.sg3.yahoo.com} 7, 34 -- Date: Mon, 14 Jul 2014 23:50:07 +0800 7, 34 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 7, 34 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 7, 34 -- Subject: Experience with Epo-Fix Epoxy as Embedding Medium for Ultramicrotome 7, 34 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 7, 34 -- MIME-Version: 1.0 7, 34 -- Content-Type: text/plain; charset=utf-8 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6EFo8wA019577 ==============================End of - Headers==============================
IMHO we need also to know which ultramicrotome (thermal or mechanical feed) you are using for this approach. Epo-Fix is known to me as a specimen mountant(mounting medium) in Materials Sciences (cf. e.g. http://www.struers.com/resources/elements/12/255648/Cold%20Mounting%20table_Epoxies.pdf ) and unfortunately I haven't experience with sectioning Epo-Fix-Epoxy embedded specimens. *) It might be this resin too soft or too hard. It might be also the type of glass strips you use and how the quality of your knives really is. Trials to overcome the problem with your glass knives (including the existing angle to be 35-45-55 degr. comparable with the diamond knives 35/45/55 degr.**)) could be: i) sectioning at a (narrower) cutting angle of say 4.5 - 5.5 degree (instead of using 6 degr.), ii) lowering level of water in the knife boat iii) playing around with section speed (ensuring that the section phase is set sufficiently long enough, i. e. start cutting at least 1 mm above block edge, end of cutting phase as short as possible below /after having sectioned through the block at its lower edge). iv) trying to harden the block further (oven over night, 65-80 degr.C.) v) last but not least - only if available - use of a diamond knife.
With the usual resins for TEM (e.g. Epon 812, Embed812, Glycidether 100 substitute (for Epon 812), LX-112 etc in former times (also using glass knives) - when mixed thoroughly according to the recommandations of LUFT (1961) and others I had no problems cutting also bigger specs at least up to 4x4 mm (for my thesis I had a really soft resin mixture for cutting whole rat hypothalami - 1day PN to 320 days of age - including the ventricle).
Would like to ask you honestly to keep us informed about your problem and eventually the solution you found (or even personal Re's you receive on this unfortunate but interesting matter), best of luck, regards Wolfgang
Wolfgang MUSS PhD EM-Lab, Pathology SALK-LKH & PMU Salzburg SALZBURG, Austria [if you need the pdf and can't download, let me know and I will send it to you -at- (rongchigram79-at-yahoo.com.sg)]
*) From: EPOFIX AND VACUUM: AN EASY METHOD TO MAKE CASTS OF HARD SUBSTRATES Jan Kresten Nielsen and Jesper Maiboe [Nielsen, Jan Kresten and Maiboe, Jesper 2000. Epofix and Vacuum: An Easy Method to Make Casts of Hard Substrates. Palaeontologia Electronica, vol. 3, issue 1, art. 2: 10pp., 1.3MB. http://palaeo-electronica.org/2000_1/epofix/issue1_00.htm Copyright: Palaeontological Association, 15 April 2000 Submission: 20 August 1999, Acceptance: 24 February 2000] see: Palaeontologia Electronica-http://www-odp.tamu.edu/paleo "The sectioning properties of Epofix mixture are good. A comparison with other embedding media is beyond the scope of this paper. The reader is referred to Bromage (1985), who provided a systematic evaluating procedure for casts and moulds." (Bromage, T. G. 1985. Systematic inquiry in tests of negative/positive replica combinations for SEM. Journal of Microscopy, 137:209-216)
**) cf. http://cime.epfl.ch/files/content/sites/cime2/files/shared/Files/Teaching/MSE_603_2013_autumn/Chapter%2012%20Sample%20prep%202013.pdf (pdf of ppt: TEM Samples preparation_D.Laub_2013 ) scroll down to foil 75: Resin: Knive angle Compression Epofix 45° 11% 35° 6% the further (following foils) 76ff perhaps also are interesting....Foil 80: Section thickness 40 - 50nm; Sectioning speed 0.2mm/sec
Searching GOOGLE for { EPOfix AND cutting quality sectioning for TEM or Transmission electron microscopy } reveals 241 results.
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Von: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] Gesendet: Montag, 14. Juli 2014 17:54 An: Muß Wolfgang Betreff: [Microscopy] Experience with Epo-Fix Epoxy as Embedding Medium for Ultramicrotome --------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear All, recently, i am trying to help a user to section her sample embedded in Epo-Fix epoxy. As i am not very experienced with ultramicrotome, I decided to section the Epo-Fix epoxy without any sample in it with a glass knife as a start.
Unfortunately, i am unable to section it to a thickness of about 100nm. What i observed is that the glass knife is only able to section at alternating interval. I learn that this can be due to either the epoxy is quite soft of [or] the glass knife is not sharp enough (or the block face might still be too big?).
The student told me that that glass transition of the epoxy is above room temperature so there shouldn't be a need to adopt cryo-ultramicrotomy approach. I also attempt to trim the block face to a small size e. g to about 1mm (or slightly less) but still the same alternate sectioning observation is made. I have tried sectioning with biological sample embedded in araldite before and there is no problem achieving thickness of 100nm or less.
My glass knife cutting angle is at 6 degree.
May i ask if i have missed out anything? Is Epo-fix suitable to be sectioned with a glass knife to achieve a thickness of 100nm? On my second question, does the Tg of the sample determine if one should use a room-temperature ultramicrotome or a cryo-ultramicrotome?
Cheers, Yee Yan, Tay FACTS Lab Nanyang Technological University
==============================Original Headers============================== 7, 34 -- From rongchigram79-at-yahoo.com.sg Mon Jul 14 10:50:10 2014 7, 34 -- Received: from nm25-vm5.bullet.mail.sg3.yahoo.com (nm25-vm5.bullet.mail.sg3.yahoo.com [106.10.151.100]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6EFo8wA019577 7, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 10:50:09 -0500 7, 34 -- Received: from [106.10.166.115] by nm25.bullet.mail.sg3.yahoo.com with NNFMP; 14 Jul 2014 15:50:08 -0000 7, 34 -- Received: from [106.10.150.24] by tm4.bullet.mail.sg3.yahoo.com with NNFMP; 14 Jul 2014 15:50:08 -0000 7, 34 -- Received: from [127.0.0.1] by omp1025.mail.sg3.yahoo.com with NNFMP; 14 Jul 2014 15:50:08 -0000 7, 34 -- X-Yahoo-Newman-Property: ymail-3 7, 34 -- X-Yahoo-Newman-Id: 122254.43108.bm-at-omp1025.mail.sg3.yahoo.com 7, 34 -- Received: (qmail 14667 invoked by uid 60001); 14 Jul 2014 15:50:08 -0000 7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1405353008; bh=g3COM9sUeNA3at/5HiMHgtmYaexuRdFVSyKntOWTBJs=; h=Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=r3wLGOTt5CIg2ptmSZBF1XnA1q4TEd90lpwfgU1IFHa402cPCxmOE4mkkoFoXvhHqAMemrEDtYoxQVjrDl3JXG8gHpdtXxrkcbO4luQ4qBje1+x8b14dEh9diLKUy81BmZ3344qHzRLALVkI1bEa2zHe50rO6QPkab54MWuUWyE= 7, 34 -- X-YMail-OSG: GgdEbuUVM1ly9bGlDonM9KyRC7.H_IWyMUAedj2UJ4t0Kpj 7, 34 -- IEgWHXNvGa5pRFvZbEWTHgv0r.gCioH10CKIBg7fVDNH5naBPvgf9SaAUAvU 7, 34 -- tjF47nItfTlbdu4UKRne3VVQnauWIF4MmABumSMod.MM5unV8bJ99o9FZYif 7, 34 -- cfIpCgsE9gRjEWNfxbIJVlu_wx7SiT4D..pZvZflFp9yTy420GYnFioDLuYV 7, 34 -- o25e0fw7Vrw3J01e0C_cSPOhX1BjL_cHq1FVkOqtDlE8lsQ_s69xfcycf.fr 7, 34 -- cfMipV.ghsYpG1DsjViuauaJHExbXRW1BusyYu1LlvnENpzVdE5jEdknACzy 7, 34 -- YIuqgAjnstO3e749jA0t2qHTbDqQcejWN8LHAgQt7NXT6CJkiGMSyW1Mlpjy 7, 34 -- W43Rex7nNleaG0wEYMwquCgEfj9cca0c2OaIJIw7My5nawWOhez6dynPsID2 7, 34 -- yAOlbJBCpOcnelE1.YTy.CMv71OIREc1LoguK5pNtRbSCmHbu1bh4i2YqmfI 7, 34 -- w1C3ONg-- 7, 34 -- Received: from [121.6.130.175] by web190102.mail.sg3.yahoo.com via HTTP; Mon, 14 Jul 2014 23:50:07 SGT 7, 34 -- X-Rocket-MIMEInfo: 002.001,RGVhciBBbGwsIHJlY2VudGx5LCBpIGFtIHRyeWluZyB0byBoZWxwIGEgdXNlciB0byBzZWN0aW9uIGhlciBzYW1wbGUgZW1iZWRkZWQgaW4gRXBvLUZpeCBlcG94eS4gQXMgaSBhbSBub3QgdmVyeSBleHBlcmllbmNlIHdpdGggdWx0cmFtaWNyb3RvbWUsIEkgZGVjaWRlIHRvIHNlY3Rpb24gdGhlIEVwby1GaXggZXBveHkgd2l0aG91dCBhbnkgc2FtcGxlIGluIGl0IHdpdGggYSBnbGFzcyBrbmlmZSBhcyBhIHN0YXJ0LiBVbmZvcnR1bmF0ZWx5ICwgaSBhbSB1bmFibGUgdG8gc2VjdGlvbiBpdCB0byBhIHRoaWMBMAEBAQE- 7, 34 -- X-Mailer: YahooMailWebService/0.8.194.680 7, 34 -- Message-ID: {1405353007.91095.YahooMailNeo-at-web190102.mail.sg3.yahoo.com} 7, 34 -- Date: Mon, 14 Jul 2014 23:50:07 +0800 7, 34 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 7, 34 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 7, 34 -- Subject: Experience with Epo-Fix Epoxy as Embedding Medium for Ultramicrotome 7, 34 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 7, 34 -- MIME-Version: 1.0 7, 34 -- Content-Type: text/plain; charset=utf-8 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6EFo8wA019577 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 40 -- From W.Muss-at-salk.at Mon Jul 14 12:03:49 2014 23, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 23, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6EH3nvF007393 23, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 12:03:49 -0500 23, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 23, 40 -- by hermes.salk.at (Postfix) with ESMTP id 80A61646B53 23, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 19:03:47 +0200 (CEST) 23, 40 -- X-Scanned-By: SALK-Content-Filter 23, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 23, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 23, 40 -- with ESMTP id Frpt3kl4zlmv for {microscopy-at-microscopy.com} ; 23, 40 -- Mon, 14 Jul 2014 19:03:47 +0200 (CEST) 23, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 23, 40 -- by hermes.salk.at (Postfix) with ESMTP id 3FC18646B46 23, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 19:03:47 +0200 (CEST) 23, 40 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 23, 40 -- by N1EX198.lks.local with ESMTP; 14 Jul 2014 19:13:30 +0200 23, 40 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 23, 40 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 23, 40 -- 14.02.0387.000; Mon, 14 Jul 2014 19:03:46 +0200 23, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 23, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 23, 40 -- Subject: [Microscopy] Re: Experience with Epo-Fix Epoxy as Embedding Medium 23, 40 -- for Ultramicrotome 23, 40 -- Thread-Topic: [Microscopy] Re: Experience with Epo-Fix Epoxy as Embedding 23, 40 -- Medium for Ultramicrotome 23, 40 -- Thread-Index: AQHPn32/4OL1fs821EC+N77JZLwbJg== 23, 40 -- Date: Mon, 14 Jul 2014 17:03:46 +0000 23, 40 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D3DBDB6-at-N2DT131.lksdom21.lks.local} 23, 40 -- References: {201407141554.s6EFs1mT023934-at-ns.microscopy.com} 23, 40 -- In-Reply-To: {201407141554.s6EFs1mT023934-at-ns.microscopy.com} 23, 40 -- Accept-Language: de-DE, en-US 23, 40 -- Content-Language: de-DE 23, 40 -- X-MS-Has-Attach: 23, 40 -- X-MS-TNEF-Correlator: 23, 40 -- x-originating-ip: [192.168.42.2] 23, 40 -- Content-Type: text/plain; charset="iso-8859-1" 23, 40 -- MIME-Version: 1.0 23, 40 -- Content-Transfer-Encoding: 8bit 23, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6EH3nvF007393 ==============================End of - Headers==============================
I have done quite a bit of imaging of SiO2 aerogel, (not nanocellulose) but some of our imaging approaches may work for you.
To image it in an SEM, I use low voltage, uncoated. Â Because of the low density of aerogel, the charge balance point is surprisingly high. Â Our aerogels are especially low density aerogels (about 20 mg/cm3, which for SiO2 is about 99.3% vacuum) and the charge point on most of our aerogels comes in at around 2.1 to 2.2 keV. Â You can get some very nice images this way. It helps to use a field emitter because of the very fine structure of the fibers, and the fact that you want to keep the current low as well as the voltage.
For sectioning aerogel, we use motorized glass needle cutting:  Westphal, A. J., Snead, C., Butterworth, A. L., Graham, G. A., Bradley, J. P., Bajt, S., et al. (2004). Aerogel keystones: Extraction of complete hypervelocity impact events from aerogel collectors. Meteoritics and Planetary Science, 39(8), 1375–1386.
This may be a lot of work to set up, but it works very, very well. Â One thing you can do is cut a 100 micron thick section, flip it on its side, and cut another section a few tens of microns thick. Â Then you can affix that to a substrate and image it uncoated with high voltage in an SEM. Â It works because the density is so low the electron beam just goes clean through. Â (Poor man's TEM.)
Finally, I have gotten images in a TEM, but since I'm not usually studying the aerogel itself (but rather minerals trapped in it) I've never developed a good protocol here. Â However, it is my experience that epoxy embedding always alters the structure of the aerogel, so you probably will be better off going with some of the other suggestions if the above doesn't work for you.
I concur with Stephan that it is possible to get a good cut with razorblades (though don't drink coffee first -- it will make your hand shake!) Â And Phil's approach seems sharp too.
Hope this helps,
Zack
} } } Ferruccio.Bolla-at-empa.ch: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear all, } } } } I am studying cellulose aerogel made from Nanocellulose Fibres (10-30nm diameter and several micron in length). } } } } MATERIAL: } } The material show highly porosity (up to 90% air) and a very intricate structure with meso and nano pores. } } } } SAMPLE: } } Samples are produced via supercritical CO2 drying after filtration and solvent exchange. The resulting sample is a ""paper-like"" fluffy material, 3 cm in diameter and a thickness of 300 micron. } } } } SEM: } } To study the microstructure I tried to embed a small piece of aerogel in araldite. The cross-section surface of the sample was exposed with a diamond microtome and sputtered with 20nm of gold. } } } } In the obtained images is difficult to distinguish between the araldite matrix and the sample (a small change in contrast at low magnification). Worst is that on the surface I don't see any micro-structure. Only a light grey smooth surface. } } } } QUESTION: } } - Is it possible in your opinion to obtain a nice cross section image of my aerogel? } } - I am thinking to change the embedding material with a more stiff epoxy. It could help? } } - Do you have any others ideas? } } } } Many thanks in advance, } } Ferruccio } } } } } } PS: This is my first message. Hope I did everything correct. } }
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Dear List In our Jeol JSEM 5600LV the filament current does not increase and after the scroll bar reaches the middle a message appears "filament burn out". Yet the filament is not burned and we can see an instant glow when the high tension is turned on. The last days the "filament burn out" message was coming on with increasing frequency and we had to turn on again and again the high tension to continue working. The Wehlnet cap and the connections of the high tension cable on the top of the gun look fine, so far I can see Any suggestion will be greatly appreciated Best regards
We recently observed some polymer aerogels in un test run for the purchase of a new SEM, and with recent SEMs it is possible to do nice things at very low voltage (300 V) in deceleration mode (the sample is positively biased to slow down the incident electrons, and the SE and BE are re-accelerated up to the detector), without any coating. It works well, with imaging up to more than 100 kx, but need some skill to find the right conditions.
For the cross-section, why not try the ion beam cross-polisher. You could obtain some test run asking one of the manufacturer (Gatan, Jeol, Hitachi, Leica Technor-Linda, etc). No embeding, possibily no metalisation.
Hope it helps !
Jacques
Le 14/07/2014 08:31, Ferruccio.Bolla-at-empa.ch a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } I am studying cellulose aerogel made from Nanocellulose Fibres (10-30nm diameter and several micron in length). } } MATERIAL: } The material show highly porosity (up to 90% air) and a very intricate structure with meso and nano pores. } } SAMPLE: } Samples are produced via supercritical CO2 drying after filtration and solvent exchange. The resulting sample is a ""paper-like"" fluffy material, 3 cm in diameter and a thickness of 300 micron. } } SEM: } To study the microstructure I tried to embed a small piece of aerogel in araldite. The cross-section surface of the sample was exposed with a diamond microtome and sputtered with 20nm of gold. } } In the obtained images is difficult to distinguish between the araldite matrix and the sample (a small change in contrast at low magnification). Worst is that on the surface I don't see any micro-structure. Only a light grey smooth surface. } } QUESTION: } - Is it possible in your opinion to obtain a nice cross section image of my aerogel? } - I am thinking to change the embedding material with a more stiff epoxy. It could help? } - Do you have any others ideas? } } Many thanks in advance, } Ferruccio } } } PS: This is my first message. Hope I did everything correct. } } } ==============================Original Headers============================== } 11, 41 -- From Ferruccio.Bolla-at-empa.ch Mon Jul 14 01:18:38 2014 } 11, 41 -- Received: from barracuda4.eawag.ch (barracuda4.eawag.ch [152.88.7.197]) } 11, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6E6Ic3m013174 } 11, 41 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 01:18:38 -0500 } 11, 41 -- X-ASG-Debug-ID: 1405318687-0814c273851bb440001-JFxeax } 11, 41 -- Received: from ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) by barracuda4.eawag.ch with ESMTP id m4myH1y77uhCEkyB for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 08:18:37 +0200 (CEST) } 11, 41 -- X-Barracuda-Envelope-From: Ferruccio.Bolla-at-empa.ch } 11, 41 -- X-Barracuda-Apparent-Source-IP: 152.88.45.32 } 11, 41 -- Received: from EE-MBX2.ee.emp-eaw.ch ([169.254.2.218]) by } 11, 41 -- ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) with mapi id 14.03.0181.006; Mon, 14 } 11, 41 -- Jul 2014 08:18:06 +0200 } 11, 41 -- From: "Bolla, Ferruccio" {Ferruccio.Bolla-at-empa.ch} } 11, 41 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 11, 41 -- Subject: SEM - Cellulose sample preparation - embedding } 11, 41 -- Thread-Topic: SEM - Cellulose sample preparation - embedding } 11, 41 -- X-ASG-Orig-Subj: SEM - Cellulose sample preparation - embedding } 11, 41 -- Thread-Index: Ac+fKyau6wTbxpSIStmaw429JY0agA== } 11, 41 -- Date: Mon, 14 Jul 2014 06:18:06 +0000 } 11, 41 -- Message-ID: {6E81EA4991422D448926980E794FEB5EE2315A-at-ee-mbx2.ee.emp-eaw.ch} } 11, 41 -- Accept-Language: de-CH, en-US } 11, 41 -- Content-Language: en-US } 11, 41 -- X-MS-Has-Attach: } 11, 41 -- X-MS-TNEF-Correlator: } 11, 41 -- x-originating-ip: [152.88.94.96] } 11, 41 -- Content-Type: text/plain; charset="us-ascii" } 11, 41 -- MIME-Version: 1.0 } 11, 41 -- X-Barracuda-Connect: UNKNOWN[152.88.45.32] } 11, 41 -- X-Barracuda-Start-Time: 1405318717 } 11, 41 -- X-Barracuda-URL: http://152.88.7.197:8000/cgi-mod/mark.cgi } 11, 41 -- X-Virus-Scanned: by bsmtpd at eawag.ch } 11, 41 -- X-Barracuda-BRTS-Status: 1 } 11, 41 -- X-Barracuda-Spam-Score: 2.80 } 11, 41 -- X-Barracuda-Spam-Status: No, SCORE=2.80 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=WEIRD_QUOTING, WEIRD_QUOTING_2 } 11, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.7498 } 11, 41 -- Rule breakdown below } 11, 41 -- pts rule name description } 11, 41 -- ---- ---------------------- -------------------------------------------------- } 11, 41 -- 0.00 WEIRD_QUOTING BODY: Weird repeated double-quotation marks } 11, 41 -- 2.80 WEIRD_QUOTING_2 Weird repeated double-quotation marks } 11, 41 -- Content-Transfer-Encoding: 8bit } 11, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6E6Ic3m013174 } ==============================End of - Headers==============================
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Dr Muss, sorry to add in the information. i am using Leica UCT. I use a mechanical feed of 100nm. At 300nm, it seems not a problem.
Cheers, Yee Yan
On Tuesday, 15 July 2014, 1:16, "W.Muss-at-salk.at" {W.Muss-at-salk.at} wrote:
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Dear Yee Yan Tay,
IMHO we need also to know which ultramicrotome (thermal or mechanical feed) you are using for this approach. Epo-Fix is known to me as a specimen mountant(mounting medium) in Materials Sciences (cf. e.g. http://www.struers.com/resources/elements/12/255648/Cold%20Mounting%20table_Epoxies.pdf ) and unfortunately I haven't experience with sectioning Epo-Fix-Epoxy embedded specimens. *) It might be this resin too soft or too hard. It might be also the type of glass strips you use and how the quality of your knives really is. Trials to overcome the problem with your glass knives (including the existing angle to be 35-45-55 degr. comparable with the diamond knives 35/45/55 degr.**)) could be: i) sectioning at a (narrower) cutting angle of say 4.5 - 5.5 degree (instead of using 6 degr.), ii) lowering level of water in the knife boat iii) playing around with section speed (ensuring that the section phase is set sufficiently long enough, i. e. start cutting at least 1 mm above block edge, end of cutting phase as short as possible below /after having sectioned through the block at its lower edge). iv) trying to harden the block further (oven over night, 65-80 degr.C.) v) last but not least - only if available - use of a diamond knife.
With the usual resins for TEM (e.g. Epon 812, Embed812, Glycidether 100 substitute (for Epon 812), LX-112 etc in former times (also using glass knives) - when mixed thoroughly according to the recommandations of LUFT (1961) and others I had no problems cutting also bigger specs at least up to 4x4 mm (for my thesis I had a really soft resin mixture for cutting whole rat hypothalami - 1day PN to 320 days of age - including the ventricle).
Would like to ask you honestly to keep us informed about your problem and eventually the solution you found (or even personal Re's you receive on this unfortunate but interesting matter), best of luck, regards Wolfgang
Wolfgang MUSS PhD EM-Lab, Pathology SALK-LKH & PMU Salzburg SALZBURG, Austria [if you need the pdf and can't download, let me know and I will send it to you -at- (rongchigram79-at-yahoo.com.sg)]
*) From: EPOFIX AND VACUUM: AN EASY METHOD TO MAKE CASTS OF HARD SUBSTRATES Jan Kresten Nielsen and Jesper Maiboe [Nielsen, Jan Kresten and Maiboe, Jesper 2000. Epofix and Vacuum: An Easy Method to Make Casts of Hard Substrates. Palaeontologia Electronica, vol. 3, issue 1, art. 2: 10pp., 1.3MB. http://palaeo-electronica.org/2000_1/epofix/issue1_00.htm Copyright: Palaeontological Association, 15 April 2000 Submission: 20 August 1999, Acceptance: 24 February 2000] see: Palaeontologia Electronica-http://www-odp.tamu.edu/paleo "The sectioning properties of Epofix mixture are good. A comparison with other embedding media is beyond the scope of this paper. The reader is referred to Bromage (1985), who provided a systematic evaluating procedure for casts and moulds." (Bromage, T. G. 1985. Systematic inquiry in tests of negative/positive replica combinations for SEM. Journal of Microscopy, 137:209-216)
**) cf. http://cime.epfl.ch/files/content/sites/cime2/files/shared/Files/Teaching/MSE_603_2013_autumn/Chapter%2012%20Sample%20prep%202013.pdf (pdf of ppt: TEM Samples preparation_D.Laub_2013 ) scroll down to foil 75: Resin: Knive angle Compression Epofix 45° 11% 35° 6% the further (following foils) 76ff perhaps also are interesting....Foil 80: Section thickness 40 - 50nm; Sectioning speed 0.2mm/sec
Searching GOOGLE for { EPOfix AND cutting quality sectioning for TEM or Transmission electron microscopy } reveals 241 results.
========================
Von: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] Gesendet: Montag, 14. Juli 2014 17:54 An: Muß Wolfgang Betreff: [Microscopy] Experience with Epo-Fix Epoxy as Embedding Medium for Ultramicrotome --------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear All, recently, i am trying to help a user to section her sample embedded in Epo-Fix epoxy. As i am not very experienced with ultramicrotome, I decided to section the Epo-Fix epoxy without any sample in it with a glass knife as a start.
Unfortunately, i am unable to section it to a thickness of about 100nm. What i observed is that the glass knife is only able to section at alternating interval. I learn that this can be due to either the epoxy is quite soft of [or] the glass knife is not sharp enough (or the block face might still be too big?).
The student told me that that glass transition of the epoxy is above room temperature so there shouldn't be a need to adopt cryo-ultramicrotomy approach. I also attempt to trim the block face to a small size e. g to about 1mm (or slightly less) but still the same alternate sectioning observation is made. I have tried sectioning with biological sample embedded in araldite before and there is no problem achieving thickness of 100nm or less.
My glass knife cutting angle is at 6 degree.
May i ask if i have missed out anything? Is Epo-fix suitable to be sectioned with a glass knife to achieve a thickness of 100nm? On my second question, does the Tg of the sample determine if one should use a room-temperature ultramicrotome or a cryo-ultramicrotome?
Cheers, Yee Yan, Tay FACTS Lab Nanyang Technological University
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==============================Original Headers============================== 23, 40 -- From W.Muss-at-salk.at Mon Jul 14 12:03:49 2014 23, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 23, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6EH3nvF007393 23, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 12:03:49 -0500 23, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 23, 40 -- by hermes.salk.at (Postfix) with ESMTP id 80A61646B53 23, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 19:03:47 +0200 (CEST) 23, 40 -- X-Scanned-By: SALK-Content-Filter 23, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 23, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 23, 40 -- with ESMTP id Frpt3kl4zlmv for {microscopy-at-microscopy.com} ; 23, 40 -- Mon, 14 Jul 2014 19:03:47 +0200 (CEST) 23, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 23, 40 -- by hermes.salk.at (Postfix) with ESMTP id 3FC18646B46 23, 40 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 19:03:47 +0200 (CEST) 23, 40 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 23, 40 -- by N1EX198.lks.local with ESMTP; 14 Jul 2014 19:13:30 +0200 23, 40 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 23, 40 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 23, 40 -- 14.02.0387.000; Mon, 14 Jul 2014 19:03:46 +0200 23, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 23, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 23, 40 -- Subject: [Microscopy] Re: Experience with Epo-Fix Epoxy as Embedding Medium 23, 40 -- for Ultramicrotome 23, 40 -- Thread-Topic: [Microscopy] Re: Experience with Epo-Fix Epoxy as Embedding 23, 40 -- Medium for Ultramicrotome 23, 40 -- Thread-Index: AQHPn32/4OL1fs821EC+N77JZLwbJg== 23, 40 -- Date: Mon, 14 Jul 2014 17:03:46 +0000 23, 40 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D3DBDB6-at-N2DT131.lksdom21.lks.local} 23, 40 -- References: {201407141554.s6EFs1mT023934-at-ns.microscopy.com} 23, 40 -- In-Reply-To: {201407141554.s6EFs1mT023934-at-ns.microscopy.com} 23, 40 -- Accept-Language: de-DE, en-US 23, 40 -- Content-Language: de-DE 23, 40 -- X-MS-Has-Attach: 23, 40 -- X-MS-TNEF-Correlator: 23, 40 -- x-originating-ip: [192.168.42.2] 23, 40 -- Content-Type: text/plain; charset="iso-8859-1" 23, 40 -- MIME-Version: 1.0 23, 40 -- Content-Transfer-Encoding: 8bit 23, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6EH3nvF007393 ==============================End of - Headers==============================
If you have old equipment to dump, consider offering it up on this forum or others. When I posted that I was taking apart an EM lab because the director had passed away, I got 30 emails from this group! People were willing to take whole instruments or any number of parts. There are many who are trying to keep old equipment going, some who can breathe new life into parts, and now several hacker spaces where kids can be exposed to all kinds of technology and can learn how to make stuff work.
If you requsted parts from me, I hope I sent you a personal email letting you know if you "won" something or not. If not, please let me apologize; I got swamped.
I found homes for a sputter coater, critical point dryer, ultramicrotome, and plasma asher, all mostly working and fairly easily repaired (I hope). Even parts from the most desperately old piece of equipment, an old Denton vacuum evaporator, will have a new life. And the turbo pump and controller, and cards and valves and gauges and manuals from the SEM have been sent to several people. I had multiple requests for each item!
So don't forget there are a lot of people out there who could benefit from pieces and parts. Nerds and hackers keep it going. And now I've managed to train/corrupt a sweet young undergrad who helped me strip everything, and she thinks it's some of the most fun she's ever had. Plus we have pictures of her dropping a turbo with which to torment her four brothers who only know how to work on mundane cars.
Reuse, recycle, and spread the knowledge and talent.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
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From reasonable-medications6-at-littleonline.com Tue Jul 15 23:19:48 2014 Return-Path: {reasonable-medications6-at-littleonline.com} Received: from smtp3.littleonline.com (239.37.58.37-pppoe-dynamic.uvttk.ru [37.58.37.239] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6G4Jk4Q025680 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 15 Jul 2014 23:19:46 -0500 Message-Id: {201407160419.s6G4Jk4Q025680-at-ns.microscopy.com}
Sorry, I made an error : to decelerate the incident beam, the sample is negatively biased, and not positively as I wrote. Shure, you have all automatically corrected my miswriting ! ;-)
Regards
Jacques
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Hi
We recently observed some polymer aerogels in un test run for the purchase of a new SEM, and with recent SEMs it is possible to do nice things at very low voltage (300 V) in deceleration mode (the sample is positively biased to slow down the incident electrons, and the SE and BE are re-accelerated up to the detector), without any coating. It works well, with imaging up to more than 100 kx, but need some skill to find the right conditions.
For the cross-section, why not try the ion beam cross-polisher. You could obtain some test run asking one of the manufacturer (Gatan, Jeol, Hitachi, Leica Technor-Linda, etc). No embeding, possibily no metalisation.
Hope it helps !
Jacques
Le 14/07/2014 08:31, Ferruccio.Bolla-at-empa.ch a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } I am studying cellulose aerogel made from Nanocellulose Fibres (10-30nm diameter and several micron in length). } } MATERIAL: } The material show highly porosity (up to 90% air) and a very intricate structure with meso and nano pores. } } SAMPLE: } Samples are produced via supercritical CO2 drying after filtration and solvent exchange. The resulting sample is a ""paper-like"" fluffy material, 3 cm in diameter and a thickness of 300 micron. } } SEM: } To study the microstructure I tried to embed a small piece of aerogel in araldite. The cross-section surface of the sample was exposed with a diamond microtome and sputtered with 20nm of gold. } } In the obtained images is difficult to distinguish between the araldite matrix and the sample (a small change in contrast at low magnification). Worst is that on the surface I don't see any micro-structure. Only a light grey smooth surface. } } QUESTION: } - Is it possible in your opinion to obtain a nice cross section image of my aerogel? } - I am thinking to change the embedding material with a more stiff epoxy. It could help? } - Do you have any others ideas? } } Many thanks in advance, } Ferruccio } } } PS: This is my first message. Hope I did everything correct. } } } ==============================Original Headers============================== } 11, 41 -- From Ferruccio.Bolla-at-empa.ch Mon Jul 14 01:18:38 2014 } 11, 41 -- Received: from barracuda4.eawag.ch (barracuda4.eawag.ch [152.88.7.197]) } 11, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6E6Ic3m013174 } 11, 41 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 01:18:38 -0500 } 11, 41 -- X-ASG-Debug-ID: 1405318687-0814c273851bb440001-JFxeax } 11, 41 -- Received: from ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) by barracuda4.eawag.ch with ESMTP id m4myH1y77uhCEkyB for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2014 08:18:37 +0200 (CEST) } 11, 41 -- X-Barracuda-Envelope-From: Ferruccio.Bolla-at-empa.ch } 11, 41 -- X-Barracuda-Apparent-Source-IP: 152.88.45.32 } 11, 41 -- Received: from EE-MBX2.ee.emp-eaw.ch ([169.254.2.218]) by } 11, 41 -- ee-hub2.ee.emp-eaw.ch ([152.88.45.32]) with mapi id 14.03.0181.006; Mon, 14 } 11, 41 -- Jul 2014 08:18:06 +0200 } 11, 41 -- From: "Bolla, Ferruccio" {Ferruccio.Bolla-at-empa.ch} } 11, 41 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 11, 41 -- Subject: SEM - Cellulose sample preparation - embedding } 11, 41 -- Thread-Topic: SEM - Cellulose sample preparation - embedding } 11, 41 -- X-ASG-Orig-Subj: SEM - Cellulose sample preparation - embedding } 11, 41 -- Thread-Index: Ac+fKyau6wTbxpSIStmaw429JY0agA== } 11, 41 -- Date: Mon, 14 Jul 2014 06:18:06 +0000 } 11, 41 -- Message-ID: {6E81EA4991422D448926980E794FEB5EE2315A-at-ee-mbx2.ee.emp-eaw.ch} } 11, 41 -- Accept-Language: de-CH, en-US } 11, 41 -- Content-Language: en-US } 11, 41 -- X-MS-Has-Attach: } 11, 41 -- X-MS-TNEF-Correlator: } 11, 41 -- x-originating-ip: [152.88.94.96] } 11, 41 -- Content-Type: text/plain; charset="us-ascii" } 11, 41 -- MIME-Version: 1.0 } 11, 41 -- X-Barracuda-Connect: UNKNOWN[152.88.45.32] } 11, 41 -- X-Barracuda-Start-Time: 1405318717 } 11, 41 -- X-Barracuda-URL: http://152.88.7.197:8000/cgi-mod/mark.cgi } 11, 41 -- X-Virus-Scanned: by bsmtpd at eawag.ch } 11, 41 -- X-Barracuda-BRTS-Status: 1 } 11, 41 -- X-Barracuda-Spam-Score: 2.80 } 11, 41 -- X-Barracuda-Spam-Status: No, SCORE=2.80 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=WEIRD_QUOTING, WEIRD_QUOTING_2 } 11, 41 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.7498 } 11, 41 -- Rule breakdown below } 11, 41 -- pts rule name description } 11, 41 -- ---- ---------------------- -------------------------------------------------- } 11, 41 -- 0.00 WEIRD_QUOTING BODY: Weird repeated double-quotation marks } 11, 41 -- 2.80 WEIRD_QUOTING_2 Weird repeated double-quotation marks } 11, 41 -- Content-Transfer-Encoding: 8bit } 11, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6E6Ic3m013174 } ==============================End of - Headers==============================
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J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
==============================Original Headers============================== 11, 26 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Jul 15 04:13:01 2014 11, 26 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6F9D0H6012217 11, 26 -- for {microscopy-at-microscopy.com} ; Tue, 15 Jul 2014 04:13:00 -0500 11, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 11, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id 193372423AC 11, 26 -- for {microscopy-at-microscopy.com} ; Tue, 15 Jul 2014 11:11:21 +0200 (CEST) 11, 26 -- X-Virus-Scanned: Debian amavisd-new at ipcms-smtp.u-strasbg.fr 11, 26 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) 11, 26 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) 11, 26 -- with ESMTP id gDH8sG9PTr7w for {microscopy-at-microscopy.com} ; 11, 26 -- Tue, 15 Jul 2014 11:11:17 +0200 (CEST) 11, 26 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 11, 26 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id EE2AF2423A2 11, 26 -- for {microscopy-at-microscopy.com} ; Tue, 15 Jul 2014 11:11:16 +0200 (CEST) 11, 26 -- Message-ID: {53C4F097.40804-at-ipcms.u-strasbg.fr} 11, 26 -- Date: Tue, 15 Jul 2014 11:12:55 +0200 11, 26 -- From: jacques faerber {jacques.faerber-at-ipcms.u-strasbg.fr} 11, 26 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 11, 26 -- MIME-Version: 1.0 11, 26 -- To: microscopy-at-microscopy.com 11, 26 -- Subject: Re: [Microscopy] SEM - Cellulose sample preparation - embedding 11, 26 -- References: {201407140631.s6E6VOGg027492-at-ns.microscopy.com} 11, 26 -- In-Reply-To: {201407140631.s6E6VOGg027492-at-ns.microscopy.com} 11, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 26 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
thank you for the information. IF there is NO mechanical problem with your Leica UCT (stepper motor, feeding) then I am pretty convinced you should be able to cut at least down to 50nm (if not 30nm). Have you considered also the room climate as a source of failure(i.e. humidity, [cold]draught)? Perhaps some shielding around the block-face - section area could be of benefit. There exists a description of errors and trouble checking for cutting from the late H. Sitte (who was one of the main inventors of the classical Reichert ultramicrotome series OMU-2,OMU-3, followed by Ultracut, Ultracut E and then UCT etc.). I shall send this pdf to you, so you perhaps can find out other sources of failure in cutting serial sections with equal thickness (specified therein esp. point 4.: "irregular section thickness").
Hope there will be any other considerations from the list.... Best wishes and good luck, sincerely, Wolfgang
} -----Ursprüngliche Nachricht----- } Von: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] } Gesendet: Dienstag, 15. Juli 2014 18:54 } An: Muß Wolfgang } Betreff: [Microscopy] Re: Re: Experience with Epo-Fix Epoxy as Embedding } Medium } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dr Muss, sorry to add in the information. i am using Leica UCT. I use a } mechanical feed of 100nm. At 300nm, it seems not a problem. } Cheers, } Yee Yan } On Tuesday, 15 July 2014, 1:16, "W.Muss-at-salk.at" {W.Muss-at-salk.at} wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } Dear Yee Yan Tay, } IMHO we need also to know which ultramicrotome (thermal or mechanical feed) } you are using for this approach. } Epo-Fix is known to me as a specimen mountant(mounting medium) in Materials } Sciences } (cf. e.g. } http://www.struers.com/resources/elements/12/255648/Cold%20Mounting%20table_Ep } oxies.pdf ) } and unfortunately I haven't experience with sectioning Epo-Fix-Epoxy embedded } specimens. *) } It might be this resin too soft or too hard. It might be also the type of } glass strips you use and how the quality of your knives really is. } Trials to overcome the problem with your glass knives (including the existing } angle to be 35-45-55 degr. comparable with the diamond knives 35/45/55 } degr.**)) could be: } i) sectioning at a (narrower) cutting angle of say 4.5 - 5.5 degree (instead } of using 6 degr.), } ii) lowering level of water in the knife boat } iii) playing around with section speed (ensuring that the section phase is set } sufficiently long enough, i. e. start cutting at least 1 mm above block edge, } end of cutting phase as short as possible below /after having sectioned } through the block at its lower edge). } iv) trying to harden the block further (oven over night, 65-80 degr.C.) } v) last but not least - only if available - use of a diamond knife. } } With the usual resins for TEM (e.g. Epon 812, Embed812, Glycidether 100 } substitute (for Epon 812), LX-112 etc in former times (also using glass } knives) - when mixed thoroughly according to the recommandations of LUFT } (1961) and others I had no problems cutting also bigger specs at least up to } 4x4 mm (for my thesis I had a really soft resin mixture for cutting whole rat } hypothalami - 1day PN to 320 days of age - including the ventricle). } } Would like to ask you honestly to keep us informed about your problem and } eventually the solution you found (or even personal Re's you receive on this } unfortunate but interesting matter), } best of luck, } regards } Wolfgang } } } Wolfgang MUSS PhD } EM-Lab, Pathology } SALK-LKH & PMU Salzburg } SALZBURG, Austria } [if you need the pdf and can't download, let me know and I will send it to you } -at- (rongchigram79-at-yahoo.com.sg)] } } } *) From: EPOFIX AND VACUUM: AN EASY METHOD TO MAKE CASTS OF HARD SUBSTRATES } Jan Kresten Nielsen and Jesper Maiboe } [Nielsen, Jan Kresten and Maiboe, Jesper 2000. Epofix and Vacuum: An Easy } Method to Make Casts of Hard Substrates. } Palaeontologia Electronica, vol. 3, issue 1, art. 2: 10pp., 1.3MB. } http://palaeo-electronica.org/2000_1/epofix/issue1_00.htm } Copyright: Palaeontological Association, 15 April 2000 } Submission: 20 August 1999, Acceptance: 24 February 2000] } see: Palaeontologia Electronica-http://www-odp.tamu.edu/paleo } "The sectioning properties of Epofix mixture are good. A comparison with other } embedding media is beyond the scope of this paper. The reader is referred to } Bromage (1985), who provided a systematic evaluating procedure for casts and } moulds." } (Bromage, T. G. 1985. Systematic inquiry in tests of negative/positive replica } combinations for SEM. Journal of Microscopy, 137:209-216) } } **) cf. } http://cime.epfl.ch/files/content/sites/cime2/files/shared/Files/Teaching/MSE_ } 603_2013_autumn/Chapter%2012%20Sample%20prep%202013.pdf } (pdf of ppt: TEM Samples preparation_D.Laub_2013 ) scroll down to foil 75: } Resin: Knive angle Compression } Epofix 45° 11% } 35° 6% } the further (following foils) 76ff perhaps also are interesting....Foil 80: } Section thickness 40 - 50nm; Sectioning speed 0.2mm/sec } } Searching GOOGLE for { EPOfix AND cutting quality sectioning for TEM or } Transmission electron microscopy } reveals 241 results. } } ======================== } } Von: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] } Gesendet: Montag, 14. Juli 2014 17:54 } An: Muß Wolfgang } Betreff: [Microscopy] Experience with Epo-Fix Epoxy as Embedding Medium for } Ultramicrotome } --------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, recently, i am trying to help a user to section her sample embedded } in Epo-Fix epoxy. } As i am not very experienced with ultramicrotome, I decided to section the } Epo-Fix epoxy without any sample in it with a glass knife as a start. } } Unfortunately, i am unable to section it to a thickness of about 100nm. } What i observed is that the glass knife is only able to section at alternating } interval. } I learn that this can be due to either the epoxy is quite soft of [or] the } glass knife is not sharp enough (or the block face might still be too big?). } } The student told me that that glass transition of the epoxy is above room } temperature so there shouldn't be a need to adopt cryo-ultramicrotomy } approach. I also attempt to trim the block face to a small size e. g to about } 1mm (or slightly less) but still the same alternate sectioning observation is } made. I have tried sectioning with biological sample embedded in araldite } before and there is no problem achieving thickness of 100nm or less. } } My glass knife cutting angle is at 6 degree. } } } May i ask if i have missed out anything? } Is Epo-fix suitable to be sectioned with a glass knife to achieve a thickness } of 100nm? } On my second question, does the Tg of the sample determine if one should use a } room-temperature ultramicrotome or a cryo-ultramicrotome? } } Cheers, } Yee Yan, Tay } FACTS Lab } Nanyang Technological University } } } } ==============================Original Headers============================== } 7, 34 -- From rongchigram79-at-yahoo.com.sg Mon Jul 14 10:50:10 2014 } 7, 34 -- Received: from nm25-vm5.bullet.mail.sg3.yahoo.com (nm25- } vm5.bullet.mail.sg3.yahoo.com [106.10.151.100]) } 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s6EFo8wA019577 } 7, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 10:50:09 -0500 } 7, 34 -- Received: from [106.10.166.115] by nm25.bullet.mail.sg3.yahoo.com } with NNFMP; 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==============================Original Headers============================== 8, 40 -- From W.Muss-at-salk.at Wed Jul 16 02:38:36 2014 8, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6G7cYMw024959 8, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 02:38:35 -0500 8, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 8, 40 -- by hermes.salk.at (Postfix) with ESMTP id 23DE0646B9B 8, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 09:38:33 +0200 (CEST) 8, 40 -- X-Scanned-By: SALK-Content-Filter 8, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 8, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 8, 40 -- with ESMTP id q2c00JNDmLVN for {microscopy-at-microscopy.com} ; 8, 40 -- Wed, 16 Jul 2014 09:38:32 +0200 (CEST) 8, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 8, 40 -- by hermes.salk.at (Postfix) with ESMTP id D091E646B97 8, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 09:38:32 +0200 (CEST) 8, 40 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 8, 40 -- by N1EX198.lks.local with ESMTP; 16 Jul 2014 09:48:15 +0200 8, 40 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 8, 40 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 8, 40 -- 14.02.0387.000; Wed, 16 Jul 2014 09:38:32 +0200 8, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 8, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 8, 40 -- Subject: [Microscopy] Re: Re: Experience with Epo-Fix Epoxy as Embedding 8, 40 -- Medium 8, 40 -- Thread-Topic: [Microscopy] Re: Re: Experience with Epo-Fix Epoxy as 8, 40 -- Embedding Medium 8, 40 -- Thread-Index: AQHPoE1pHBHZAfkha0+5zFwcc/DVLZuiTRBg 8, 40 -- Date: Wed, 16 Jul 2014 07:38:31 +0000 8, 40 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D3E0EF4-at-N2DT131.lksdom21.lks.local} 8, 40 -- References: {201407151654.s6FGs7Ht010403-at-ns.microscopy.com} 8, 40 -- In-Reply-To: {201407151654.s6FGs7Ht010403-at-ns.microscopy.com} 8, 40 -- Accept-Language: de-DE, en-US 8, 40 -- Content-Language: de-DE 8, 40 -- X-MS-Has-Attach: 8, 40 -- X-MS-TNEF-Correlator: 8, 40 -- x-originating-ip: [192.168.42.2] 8, 40 -- Content-Type: text/plain; charset="iso-8859-1" 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6G7cYMw024959 ==============================End of - Headers==============================
Dear all, in my reply below I wrote " "late H. Sitte". I apologize for this my "semantic" fault, since I meant "retired Prof. H. Sitte"
John Minter sent me an e-mail regarding this untrue fact and so I cann tell:
Prof. Helmuth Sitte has celebrated his 85th birthday on 6th of May 2103. His company still is protocolled (perhaps also his living town and address) at Dr. Hellmuth Sitte Reitherspitzstraße 166 A-6100 Seefeld Austria
I confused here the death of his brother I think as well as the closure of the famous courses on Preparation techniques in (T)EM. So, I guess this is really a big mistake I have to correct via MSA-Listserver
Regards Wolfgang
Von: W.Muss-at-salk.at [mailto:W.Muss-at-salk.at] Gesendet: Mittwoch, 16. Juli 2014 09:44 An: Muß Wolfgang Betreff: CTRL_[Microscopy] Re to MSA-Listserver: Re: Experience with Epo-Fix Epoxy as Embedding
--------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Yee Yan,
thank you for the information. IF there is NO mechanical problem with your Leica UCT (stepper motor, feeding) then I am pretty convinced you should be able to cut at least down to 50nm (if not 30nm). Have you considered also the room climate as a source of failure (i.e. humidity, [cold]draught)? Perhaps some shielding around the block-face - section area could be of benefit. There exists a description of errors and trouble checking for cutting from the late H. Sitte (who was one of the main inventors of the classical Reichert ultramicrotome series OMU-2, OMU-3, followed by Ultracut, Ultracut E and then UCT etc.). I shall send this pdf to you, so you perhaps can find out other sources of failure in cutting serial sections with equal thickness (specified therein esp. point 4.: "irregular section thickness").
Hope there will be any other considerations from the list.... Best wishes and good luck, sincerely, Wolfgang
} -----Ursprüngliche Nachricht----- } Von: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] } Gesendet: Dienstag, 15. Juli 2014 18:54 } An: Muß Wolfgang } Betreff: [Microscopy] Re: Re: Experience with Epo-Fix Epoxy as Embedding } Medium } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dr Muss, sorry to add in the information. i am using Leica UCT. I use a } mechanical feed of 100nm. At 300nm, it seems not a problem. } Cheers, } Yee Yan } On Tuesday, 15 July 2014, 1:16, "W.Muss-at-salk.at" {W.Muss-at-salk.at} wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } Dear Yee Yan Tay, } IMHO we need also to know which ultramicrotome (thermal or mechanical feed) } you are using for this approach. } Epo-Fix is known to me as a specimen mountant(mounting medium) in Materials } Sciences } (cf. e.g. } http://www.struers.com/resources/elements/12/255648/Cold%20Mounting%20table_Ep } oxies.pdf ) } and unfortunately I haven't experience with sectioning Epo-Fix-Epoxy embedded } specimens. *) } It might be this resin too soft or too hard. It might be also the type of } glass strips you use and how the quality of your knives really is. } Trials to overcome the problem with your glass knives (including the existing } angle to be 35-45-55 degr. comparable with the diamond knives 35/45/55 } degr.**)) could be: } i) sectioning at a (narrower) cutting angle of say 4.5 - 5.5 degree (instead } of using 6 degr.), } ii) lowering level of water in the knife boat } iii) playing around with section speed (ensuring that the section phase is set } sufficiently long enough, i. e. start cutting at least 1 mm above block edge, } end of cutting phase as short as possible below /after having sectioned } through the block at its lower edge). } iv) trying to harden the block further (oven over night, 65-80 degr.C.) } v) last but not least - only if available - use of a diamond knife. } } With the usual resins for TEM (e.g. Epon 812, Embed812, Glycidether 100 } substitute (for Epon 812), LX-112 etc in former times (also using glass } knives) - when mixed thoroughly according to the recommandations of LUFT } (1961) and others I had no problems cutting also bigger specs at least up to } 4x4 mm (for my thesis I had a really soft resin mixture for cutting whole rat } hypothalami - 1day PN to 320 days of age - including the ventricle). } } Would like to ask you honestly to keep us informed about your problem and } eventually the solution you found (or even personal Re's you receive on this } unfortunate but interesting matter), } best of luck, } regards } Wolfgang } } } Wolfgang MUSS PhD } EM-Lab, Pathology } SALK-LKH & PMU Salzburg } SALZBURG, Austria } [if you need the pdf and can't download, let me know and I will send it to you } -at- (rongchigram79-at-yahoo.com.sg)] } } } *) From: EPOFIX AND VACUUM: AN EASY METHOD TO MAKE CASTS OF HARD SUBSTRATES } Jan Kresten Nielsen and Jesper Maiboe } [Nielsen, Jan Kresten and Maiboe, Jesper 2000. Epofix and Vacuum: An Easy } Method to Make Casts of Hard Substrates. } Palaeontologia Electronica, vol. 3, issue 1, art. 2: 10pp., 1.3MB. } http://palaeo-electronica.org/2000_1/epofix/issue1_00.htm } Copyright: Palaeontological Association, 15 April 2000 } Submission: 20 August 1999, Acceptance: 24 February 2000] } see: Palaeontologia Electronica-http://www-odp.tamu.edu/paleo } "The sectioning properties of Epofix mixture are good. A comparison with other } embedding media is beyond the scope of this paper. The reader is referred to } Bromage (1985), who provided a systematic evaluating procedure for casts and } moulds." } (Bromage, T. G. 1985. Systematic inquiry in tests of negative/positive replica } combinations for SEM. Journal of Microscopy, 137:209-216) } } **) cf. } http://cime.epfl.ch/files/content/sites/cime2/files/shared/Files/Teaching/MSE_ } 603_2013_autumn/Chapter%2012%20Sample%20prep%202013.pdf } (pdf of ppt: TEM Samples preparation_D.Laub_2013 ) scroll down to foil 75: } Resin: Knive angle Compression } Epofix 45° 11% } 35° 6% } the further (following foils) 76ff perhaps also are interesting....Foil 80: } Section thickness 40 - 50nm; Sectioning speed 0.2mm/sec } } Searching GOOGLE for { EPOfix AND cutting quality sectioning for TEM or } Transmission electron microscopy } reveals 241 results. } } ======================== } } Von: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] } Gesendet: Montag, 14. Juli 2014 17:54 } An: Muß Wolfgang } Betreff: [Microscopy] Experience with Epo-Fix Epoxy as Embedding Medium for } Ultramicrotome } --------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, recently, i am trying to help a user to section her sample embedded } in Epo-Fix epoxy. } As i am not very experienced with ultramicrotome, I decided to section the } Epo-Fix epoxy without any sample in it with a glass knife as a start. } } Unfortunately, i am unable to section it to a thickness of about 100nm. } What i observed is that the glass knife is only able to section at alternating } interval. } I learn that this can be due to either the epoxy is quite soft of [or] the } glass knife is not sharp enough (or the block face might still be too big?). } } The student told me that that glass transition of the epoxy is above room } temperature so there shouldn't be a need to adopt cryo-ultramicrotomy } approach. I also attempt to trim the block face to a small size e. g to about } 1mm (or slightly less) but still the same alternate sectioning observation is } made. I have tried sectioning with biological sample embedded in araldite } before and there is no problem achieving thickness of 100nm or less. } } My glass knife cutting angle is at 6 degree. } } } May i ask if i have missed out anything? } Is Epo-fix suitable to be sectioned with a glass knife to achieve a thickness } of 100nm? } On my second question, does the Tg of the sample determine if one should use a } room-temperature ultramicrotome or a cryo-ultramicrotome? } } Cheers, } Yee Yan, Tay } FACTS Lab } Nanyang Technological University } } } } ==============================Original Headers============================== } 7, 34 -- From rongchigram79-at-yahoo.com.sg Mon Jul 14 10:50:10 2014 } 7, 34 -- Received: from nm25-vm5.bullet.mail.sg3.yahoo.com (nm25- } vm5.bullet.mail.sg3.yahoo.com [106.10.151.100]) } 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s6EFo8wA019577 } 7, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 10:50:09 -0500 } 7, 34 -- Received: from [106.10.166.115] by nm25.bullet.mail.sg3.yahoo.com } with NNFMP; 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==============================Original Headers============================== 8, 40 -- From W.Muss-at-salk.at Wed Jul 16 02:38:36 2014 8, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6G7cYMw024959 8, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 02:38:35 -0500 8, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 8, 40 -- by hermes.salk.at (Postfix) with ESMTP id 23DE0646B9B 8, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 09:38:33 +0200 (CEST) 8, 40 -- X-Scanned-By: SALK-Content-Filter 8, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 8, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 8, 40 -- with ESMTP id q2c00JNDmLVN for {microscopy-at-microscopy.com} ; 8, 40 -- Wed, 16 Jul 2014 09:38:32 +0200 (CEST) 8, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 8, 40 -- by hermes.salk.at (Postfix) with ESMTP id D091E646B97 8, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 09:38:32 +0200 (CEST) 8, 40 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 8, 40 -- by N1EX198.lks.local with ESMTP; 16 Jul 2014 09:48:15 +0200 8, 40 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 8, 40 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 8, 40 -- 14.02.0387.000; Wed, 16 Jul 2014 09:38:32 +0200 8, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 8, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 8, 40 -- Subject: [Microscopy] Re: Re: Experience with Epo-Fix Epoxy as Embedding 8, 40 -- Medium 8, 40 -- Thread-Topic: [Microscopy] Re: Re: Experience with Epo-Fix Epoxy as 8, 40 -- Embedding Medium 8, 40 -- Thread-Index: AQHPoE1pHBHZAfkha0+5zFwcc/DVLZuiTRBg 8, 40 -- Date: Wed, 16 Jul 2014 07:38:31 +0000 8, 40 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D3E0EF4-at-N2DT131.lksdom21.lks.local} 8, 40 -- References: {201407151654.s6FGs7Ht010403-at-ns.microscopy.com} 8, 40 -- In-Reply-To: {201407151654.s6FGs7Ht010403-at-ns.microscopy.com} 8, 40 -- Accept-Language: de-DE, en-US 8, 40 -- Content-Language: de-DE 8, 40 -- X-MS-Has-Attach: 8, 40 -- X-MS-TNEF-Correlator: 8, 40 -- x-originating-ip: [192.168.42.2] 8, 40 -- Content-Type: text/plain; charset="iso-8859-1" 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6G7cYMw024959 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 40 -- From W.Muss-at-salk.at Wed Jul 16 06:21:11 2014 17, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 17, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6GBLAI4016001 17, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 06:21:10 -0500 17, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 17, 40 -- by hermes.salk.at (Postfix) with ESMTP id 38081646B4D 17, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 13:21:09 +0200 (CEST) 17, 40 -- X-Scanned-By: SALK-Content-Filter 17, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 17, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 17, 40 -- with ESMTP id ACQJBACnWnuO for {microscopy-at-microscopy.com} ; 17, 40 -- Wed, 16 Jul 2014 13:21:09 +0200 (CEST) 17, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 17, 40 -- by hermes.salk.at (Postfix) with ESMTP id 062ED646B49 17, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 13:21:09 +0200 (CEST) 17, 40 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 17, 40 -- by N1EX198.lks.local with ESMTP; 16 Jul 2014 13:30:52 +0200 17, 40 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 17, 40 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 17, 40 -- 14.02.0387.000; Wed, 16 Jul 2014 13:21:08 +0200 17, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 17, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 17, 40 -- Subject: [Microscopy] Re:CORRECTION of my recent reply_ Re: Experience with 17, 40 -- Epo-Fix Epoxy as Embedding 17, 40 -- Thread-Topic: [Microscopy] Re:CORRECTION of my recent reply_ Re: Experience 17, 40 -- with Epo-Fix Epoxy as Embedding 17, 40 -- Thread-Index: AQHPoMm9wOMdSA6nyU6/j6Rq32OIfpuijZOQ 17, 40 -- Date: Wed, 16 Jul 2014 11:21:08 +0000 17, 40 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D3E1C61-at-N2DT131.lksdom21.lks.local} 17, 40 -- References: {201407160744.s6G7i5sa030559-at-ns.microscopy.com} 17, 40 -- In-Reply-To: {201407160744.s6G7i5sa030559-at-ns.microscopy.com} 17, 40 -- Accept-Language: de-DE, en-US 17, 40 -- Content-Language: de-DE 17, 40 -- X-MS-Has-Attach: 17, 40 -- X-MS-TNEF-Correlator: 17, 40 -- x-originating-ip: [192.168.42.2] 17, 40 -- Content-Type: text/plain; charset="iso-8859-1" 17, 40 -- MIME-Version: 1.0 17, 40 -- Content-Transfer-Encoding: 8bit 17, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6GBLAI4016001 ==============================End of - Headers==============================
From express_meds8-at-hitv.ru Wed Jul 16 06:46:50 2014 Return-Path: {express_meds8-at-hitv.ru} Received: from 217-66-22-8.hitv.ru (217-66-22-8.hitv.ru [217.66.22.8] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6GBkjgh030298 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 16 Jul 2014 06:46:46 -0500 Message-ID: {8033BD10.2188278-at-hitv.ru}
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Email: iskandar-at-gfe.rwth-aachen.de Name: Mohamad Riza Iskandar
Organization: Central Facility for Electron Microscopy RWTH Aachen University
Title-Subject: [Filtered] Running Digital Micrograph on Mac OSX
Message: Dear all,
I'm trying to run Digital Micrograph on my Mac (10.9.4) but unfortunately I get problem with the installation process. I've followed the direction as written in one article in Microscopy today (March 2012) but I always get problem when installing the Digital Micrograph. The installation process always stop with an error message of "Removing device DMVIDEO, cleanup FAILURE". I did install everything I knew as prerequisite as mentioned in that article, but still no luck.
I would appreciate if someone can help me to solve this problem. Any suggestions via private message are also welcome.
Thank you in advanced,
Best regards,
Riza Iskandar
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Jul 16 07:04:13 2014 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6GC4DDB003155 18, 28 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 07:04:13 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id CA20F72E07A 18, 28 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 07:04:13 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id y1AJWBuEgTcG for {microscopy-at-microscopy.com} ; 18, 28 -- Wed, 16 Jul 2014 07:04:08 -0500 (CDT) 18, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id 7A0FF72E068 18, 28 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 07:04:08 -0500 (CDT) 18, 28 -- Message-ID: {53C66A38.5050002-at-microscopy.com} 18, 28 -- Date: Wed, 16 Jul 2014 07:04:08 -0500 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: viaWWW:Running Digital Micrograph on Mac OSX 18, 28 -- References: {201407160824.s6G8OrjO013429-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201407160824.s6G8OrjO013429-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201407160824.s6G8OrjO013429-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Prof Muss, thank you very much for your kindness in providing all the valuable advices and experiences! Let me read them slowly and try on my machine. I also have a Leica UC7 to try on. My country is hot and humid but the lab is kept at 40-50% humidity. I am not sure if it will contribute to the degrading of the Epo-fix.
Thank you once again!!
Cheers, Yee Yan
On Wednesday, July 16, 2014 3:59 PM, "W.Muss-at-salk.at" {W.Muss-at-salk.at} wrote:
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Dear Yee Yan,
thank you for the information. IF there is NO mechanical problem with your Leica UCT (stepper motor, feeding) then I am pretty convinced you should be able to cut at least down to 50nm (if not 30nm). Have you considered also the room climate as a source of failure(i.e. humidity, [cold]draught)? Perhaps some shielding around the block-face - section area could be of benefit. There exists a description of errors and trouble checking for cutting from the late H. Sitte (who was one of the main inventors of the classical Reichert ultramicrotome series OMU-2,OMU-3, followed by Ultracut, Ultracut E and then UCT etc.). I shall send this pdf to you, so you perhaps can find out other sources of failure in cutting serial sections with equal thickness (specified therein esp. point 4.: "irregular section thickness").
Hope there will be any other considerations from the list.... Best wishes and good luck, sincerely, Wolfgang
} -----Ursprüngliche Nachricht----- } Von: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] } Gesendet: Dienstag, 15. Juli 2014 18:54 } An: Muß Wolfgang } Betreff: [Microscopy] Re: Re: Experience with Epo-Fix Epoxy as Embedding } Medium } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dr Muss, sorry to add in the information. i am using Leica UCT. I use a } mechanical feed of 100nm. At 300nm, it seems not a problem. } Cheers, } Yee Yan } On Tuesday, 15 July 2014, 1:16, "W.Muss-at-salk.at" {W.Muss-at-salk.at} wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } Dear Yee Yan Tay, } IMHO we need also to know which ultramicrotome (thermal or mechanical feed) } you are using for this approach. } Epo-Fix is known to me as a specimen mountant(mounting medium) in Materials } Sciences } (cf. e.g. } http://www.struers.com/resources/elements/12/255648/Cold%20Mounting%20table_Ep } oxies.pdf ) } and unfortunately I haven't experience with sectioning Epo-Fix-Epoxy embedded } specimens. *) } It might be this resin too soft or too hard. It might be also the type of } glass strips you use and how the quality of your knives really is. } Trials to overcome the problem with your glass knives (including the existing } angle to be 35-45-55 degr. comparable with the diamond knives 35/45/55 } degr.**)) could be: } i) sectioning at a (narrower) cutting angle of say 4.5 - 5.5 degree (instead } of using 6 degr.), } ii) lowering level of water in the knife boat } iii) playing around with section speed (ensuring that the section phase is set } sufficiently long enough, i. e. start cutting at least 1 mm above block edge, } end of cutting phase as short as possible below /after having sectioned } through the block at its lower edge). } iv) trying to harden the block further (oven over night, 65-80 degr.C.) } v) last but not least - only if available - use of a diamond knife. } } With the usual resins for TEM (e.g. Epon 812, Embed812, Glycidether 100 } substitute (for Epon 812), LX-112 etc in former times (also using glass } knives) - when mixed thoroughly according to the recommandations of LUFT } (1961) and others I had no problems cutting also bigger specs at least up to } 4x4 mm (for my thesis I had a really soft resin mixture for cutting whole rat } hypothalami - 1day PN to 320 days of age - including the ventricle). } } Would like to ask you honestly to keep us informed about your problem and } eventually the solution you found (or even personal Re's you receive on this } unfortunate but interesting matter), } best of luck, } regards } Wolfgang } } } Wolfgang MUSS PhD } EM-Lab, Pathology } SALK-LKH & PMU Salzburg } SALZBURG, Austria } [if you need the pdf and can't download, let me know and I will send it to you } -at- (rongchigram79-at-yahoo.com.sg)] } } } *) From: EPOFIX AND VACUUM: AN EASY METHOD TO MAKE CASTS OF HARD SUBSTRATES } Jan Kresten Nielsen and Jesper Maiboe } [Nielsen, Jan Kresten and Maiboe, Jesper 2000. Epofix and Vacuum: An Easy } Method to Make Casts of Hard Substrates. } Palaeontologia Electronica, vol. 3, issue 1, art. 2: 10pp., 1.3MB. } http://palaeo-electronica.org/2000_1/epofix/issue1_00.htm } Copyright: Palaeontological Association, 15 April 2000 } Submission: 20 August 1999, Acceptance: 24 February 2000] } see: Palaeontologia Electronica-http://www-odp.tamu.edu/paleo } "The sectioning properties of Epofix mixture are good. A comparison with other } embedding media is beyond the scope of this paper. The reader is referred to } Bromage (1985), who provided a systematic evaluating procedure for casts and } moulds." } (Bromage, T. G. 1985. Systematic inquiry in tests of negative/positive replica } combinations for SEM. Journal of Microscopy, 137:209-216) } } **) cf. } http://cime.epfl.ch/files/content/sites/cime2/files/shared/Files/Teaching/MSE_ } 603_2013_autumn/Chapter%2012%20Sample%20prep%202013.pdf } (pdf of ppt: TEM Samples preparation_D.Laub_2013 ) scroll down to foil 75: } Resin: Knive angle Compression } Epofix 45° 11% } 35° 6% } the further (following foils) 76ff perhaps also are interesting....Foil 80: } Section thickness 40 - 50nm; Sectioning speed 0.2mm/sec } } Searching GOOGLE for { EPOfix AND cutting quality sectioning for TEM or } Transmission electron microscopy } reveals 241 results. } } ======================== } } Von: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] } Gesendet: Montag, 14. Juli 2014 17:54 } An: Muß Wolfgang } Betreff: [Microscopy] Experience with Epo-Fix Epoxy as Embedding Medium for } Ultramicrotome } --------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, recently, i am trying to help a user to section her sample embedded } in Epo-Fix epoxy. } As i am not very experienced with ultramicrotome, I decided to section the } Epo-Fix epoxy without any sample in it with a glass knife as a start. } } Unfortunately, i am unable to section it to a thickness of about 100nm. } What i observed is that the glass knife is only able to section at alternating } interval. } I learn that this can be due to either the epoxy is quite soft of [or] the } glass knife is not sharp enough (or the block face might still be too big?). } } The student told me that that glass transition of the epoxy is above room } temperature so there shouldn't be a need to adopt cryo-ultramicrotomy } approach. I also attempt to trim the block face to a small size e. g to about } 1mm (or slightly less) but still the same alternate sectioning observation is } made. I have tried sectioning with biological sample embedded in araldite } before and there is no problem achieving thickness of 100nm or less. } } My glass knife cutting angle is at 6 degree. } } } May i ask if i have missed out anything? } Is Epo-fix suitable to be sectioned with a glass knife to achieve a thickness } of 100nm? } On my second question, does the Tg of the sample determine if one should use a } room-temperature ultramicrotome or a cryo-ultramicrotome? } } Cheers, } Yee Yan, Tay } FACTS Lab } Nanyang Technological University } } } } ==============================Original Headers============================== } 7, 34 -- From rongchigram79-at-yahoo.com.sg Mon Jul 14 10:50:10 2014 } 7, 34 -- Received: from nm25-vm5.bullet.mail.sg3.yahoo.com (nm25- } vm5.bullet.mail.sg3.yahoo.com [106.10.151.100]) } 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s6EFo8wA019577 } 7, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Jul 2014 10:50:09 -0500 } 7, 34 -- Received: from [106.10.166.115] by nm25.bullet.mail.sg3.yahoo.com } with NNFMP; 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charset=iso-8859-1 } 36, 53 -- Content-Transfer-Encoding: 8bit } 36, 53 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s6FGig1M005955 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 40 -- From W.Muss-at-salk.at Wed Jul 16 02:38:36 2014 8, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6G7cYMw024959 8, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 02:38:35 -0500 8, 40 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 8, 40 -- by hermes.salk.at (Postfix) with ESMTP id 23DE0646B9B 8, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 09:38:33 +0200 (CEST) 8, 40 -- X-Scanned-By: SALK-Content-Filter 8, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 8, 40 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 8, 40 -- with ESMTP id q2c00JNDmLVN for {microscopy-at-microscopy.com} ; 8, 40 -- Wed, 16 Jul 2014 09:38:32 +0200 (CEST) 8, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 8, 40 -- by hermes.salk.at (Postfix) with ESMTP id D091E646B97 8, 40 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2014 09:38:32 +0200 (CEST) 8, 40 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 8, 40 -- by N1EX198.lks.local with ESMTP; 16 Jul 2014 09:48:15 +0200 8, 40 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 8, 40 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 8, 40 -- 14.02.0387.000; Wed, 16 Jul 2014 09:38:32 +0200 8, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 8, 40 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 8, 40 -- Subject: [Microscopy] Re: Re: Experience with Epo-Fix Epoxy as Embedding 8, 40 -- Medium 8, 40 -- Thread-Topic: [Microscopy] Re: Re: Experience with Epo-Fix Epoxy as 8, 40 -- Embedding Medium 8, 40 -- Thread-Index: AQHPoE1pHBHZAfkha0+5zFwcc/DVLZuiTRBg 8, 40 -- Date: Wed, 16 Jul 2014 07:38:31 +0000 8, 40 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D3E0EF4-at-N2DT131.lksdom21.lks.local} 8, 40 -- References: {201407151654.s6FGs7Ht010403-at-ns.microscopy.com} 8, 40 -- In-Reply-To: {201407151654.s6FGs7Ht010403-at-ns.microscopy.com} 8, 40 -- Accept-Language: de-DE, en-US 8, 40 -- Content-Language: de-DE 8, 40 -- X-MS-Has-Attach: 8, 40 -- X-MS-TNEF-Correlator: 8, 40 -- x-originating-ip: [192.168.42.2] 8, 40 -- Content-Type: text/plain; charset="iso-8859-1" 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6G7cYMw024959 ==============================End of - Headers==============================
From pharmacy-express1-at-acbrecovery.com Wed Jul 16 22:49:24 2014 Return-Path: {pharmacy-express1-at-acbrecovery.com} Received: from smtp3.acbrecovery.com ([222.218.124.212]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6H3nLZQ023224 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 16 Jul 2014 22:49:22 -0500 Message-Id: {201407170349.s6H3nLZQ023224-at-ns.microscopy.com}
We are having internal discussions about the structure and support for our microscopy facility and I would like to take an informal poll of others who run departmental or campus microscopy facilities.
1. Does your institution support in part or fully, a facility scientist(s) to help run the facility day to day 2. Do you receive some form of compensation as the person in charge of the facility in terms of salary or reduced teaching or other? 3. Does your institution help support repairs or service contracts for instruments?
Will compile results and post if desired. Any extra information appreciated. Thanks- Dave
Dr. David Knecht Professor of Molecular and Cell Biology Core Microscopy Facility Director University of Connecticut Storrs, CT 06269 860-486-2200
We have many fume hoods in the department but no perchloric hood. I'm looking for an electro polisher recipe for making TEM foils of nickel-chrome-aluminum based superalloys that doesn't require perchloric acid.
I appreciate any help you can lend.
Cheers Owen
-- Owen P Mills Michigan Technological University 1400 Townsend Dr Houghton, MI 49931 906-369-1875
The Advanced Imaging Center (AIC) at HHMI Janelia Research Campus is jointly sponsored by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation. Our mission is to make cutting-edge, pre-commercial microscopes developed at Janelia available to visiting scientists, maximizing the impact of the latest developments in optical instruments and emerging microscopy technologies.
We encourage applications from scientists who are addressing significant scientific questions that require measurements of cellular/molecular behavior at spatial and/or temporal resolutions that would only be possible for them through access to the AIC.
Under the Program, visiting scientists will spend 1-2 weeks (or longer if there is justified scientific reason) at Janelia to conduct experiments on the scope with the support of the AIC team.
The currently available microscopes, which provide advanced capabilities that are not yet widely available, are:
Our website, http://www.janelia.org/aic provides more details about these unique instruments.
Our first Call-for-Proposals begins today, July 15, 2014, and closes on August 15, 2014. Proposals will be evaluated based on scientific merit through a two-tier review process by AIC/Janelia scientists and by a review panel of scientists from HHMI/Janelia, GBMF, and extramural imaging experts. Additional information and instructions on how apply are available here: http://www.janelia.org/aic
Scientists whose proposals are selected will travel to Janelia to use the facility. The Janelia Visitor Program will cover the cost of lodging for the visiting scientist, experiments, technical support from our applications scientists, and scope time.
Email aic-at-janelia.hhmi.org or me with direct inquiries.
Please help us by spreading the word to your colleagues. Here is the link to the HHMI press release. http://tinyurl.com/ncbwwbp
Regards,
Teng-Leong Chew, PhD Director, Advanced Imaging Center Howard Hughes Medical Institute at Janelia Ashburn, VA 20147 (571) 209-4676 chewt-at-janelia.hhmi.org
==============================Original Headers============================== 17, 30 -- From prvs=2681f9ffc=chewt-at-janelia.hhmi.org Thu Jul 17 12:13:07 2014 17, 30 -- Received: from MAIL1.HHMI.ORG (mail1.hhmi.org [206.241.3.57]) 17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6HHD5Xb022285 17, 30 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jul 2014 12:13:06 -0500 17, 30 -- X-IronPort-AV: E=Sophos;i="5.01,679,1400040000"; 17, 30 -- d="scan'208";a="30283282" 17, 30 -- Received: from unknown (HELO JFCA2.hhmi.org) ([172.19.15.142]) 17, 30 -- by MAIL1.HHMI.ORG with ESMTP; 17 Jul 2014 13:13:05 -0400 17, 30 -- Received: from JFMB1.hhmi.org ([fe80::4933:8df9:ca67:70fc]) by JFCA2.hhmi.org 17, 30 -- ([fe80::5ce8:42db:7560:474b%17]) with mapi id 14.02.0328.009; Thu, 17 Jul 17, 30 -- 2014 13:13:05 -0400 17, 30 -- From: "Chew, Teng-Leong" {chewt-at-janelia.hhmi.org} 17, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 17, 30 -- Subject: LM - Call-for-Proposals: Advanced Imaging Center at HHMI Janelia 17, 30 -- Thread-Topic: LM - Call-for-Proposals: Advanced Imaging Center at HHMI 17, 30 -- Janelia 17, 30 -- Thread-Index: AQHPoeJfJNu1TbJDi0iJq0avx+BIJw== 17, 30 -- Date: Thu, 17 Jul 2014 17:13:04 +0000 17, 30 -- Message-ID: {674FF93F85D23B4D8EC866E532550C16647B872A-at-JFMB1.hhmi.org} 17, 30 -- Accept-Language: en-US 17, 30 -- Content-Language: en-US 17, 30 -- X-MS-Has-Attach: 17, 30 -- X-MS-TNEF-Correlator: 17, 30 -- user-agent: Microsoft-MacOutlook/14.4.3.140616 17, 30 -- x-originating-ip: [10.40.2.12] 17, 30 -- Content-Type: text/plain; charset="us-ascii" 17, 30 -- Content-ID: {054C1F5C08E7924693416B8F1D222859-at-hhmi.org} 17, 30 -- MIME-Version: 1.0 17, 30 -- Content-Transfer-Encoding: 8bit 17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6HHD5Xb022285 ==============================End of - Headers==============================
From drugs-canadian10-at-hinet.net Thu Jul 17 13:52:58 2014 Return-Path: {drugs-canadian10-at-hinet.net} Received: from 114-25-5-216.dynamic.hinet.net (114-25-5-216.dynamic.hinet.net [114.25.5.216]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6HIquRK027762 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 17 Jul 2014 13:52:57 -0500 Message-ID: {69627D6D.9473484-at-hinet.net}
X-from: gary-at-gaugler.com ()
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Email: gary-at-gaugler.com Name: Dr Gary Gaugler
Organization: Microtechnics, Inc.
Title-Subject: [Filtered] SFEG SEMs
Message: Hello Listers:
I am looking for a used SFEG and know some makes and models but could use some help to round out the search list. First, did LEO make a SFEG before the Zeiss Supra40 and if so, what model is it? Second, are there any small/medium chamber size SFEG SEMs like the Supra40 chamber from other makers? The largest chamber system I had was the Zeiss Supra55. Nice but not that big of chamber needed. I'm doing pin stubs and metallurgical coupons. High resolution, EDS and mag up to 300KX with dual SE detectors and BSD are about it. I think that Hitachi only does CFE but JEOL does CFE and TFE. I'd like to get a system up and running by the end of this year or early next year depending on what I can find with PC control and is affordable. All inputs are appreciated.
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jul 17 21:44:38 2014 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6I2ich8005072 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jul 2014 21:44:38 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 54FE272F2B9 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jul 2014 21:44:38 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id UXWvN5HRyc-5 for {microscopy-at-microscopy.com} ; 11, 28 -- Thu, 17 Jul 2014 21:44:32 -0500 (CDT) 11, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 40B3C72F2AC 11, 28 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jul 2014 21:44:32 -0500 (CDT) 11, 28 -- Message-ID: {53C88A10.70405-at-microscopy.com} 11, 28 -- Date: Thu, 17 Jul 2014 21:44:32 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:SFEG SEMs 11, 28 -- References: {201407172132.s6HLWVFu029818-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201407172132.s6HLWVFu029818-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201407172132.s6HLWVFu029818-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From affordable-drugstore10-at-jeffco.k12.co.us Thu Jul 17 23:00:48 2014 Return-Path: {affordable-drugstore10-at-jeffco.k12.co.us} Received: from smtp1.jeffco.k12.co.us ([46.36.142.182]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6I40jTU025577 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 17 Jul 2014 23:00:46 -0500 Message-Id: {201407180400.s6I40jTU025577-at-ns.microscopy.com}
Dear listers,
I'd like to have your opinion about the use or non-use of sodium borohydride in the procedure for preparing cells or tissue for immunology. The fixative that has been used is 4% paraformaldehyde and 0.1% glutaraldehyde in phosphate buffer which give us better membranes rather than not using any glutaraldehyde.
I know that the sodium borohydride changes the unbound aldehyde to an alcohol but how much aldehyde can be left in the cells after all the washes?
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced. Pat Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491 connellyps-at-mail.nih.gov http://www.nhlbi.nih.gov/research/intramural/researchers/core/electron-microscopy-core/index.html
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I am looking to purchase a new dimpling grinder, I've only used the Gatan model but am curious about the VCR and Fischione products. If you have any comments on the tools or comparison between manufacturers could you please contact me off list?
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Email: vwporscherxr-at-gmail.com Name: Rod Rowland
Organization: MATSYS, Inc.
Title-Subject: [Filtered] Imaging metal powders
Message: After a couple of years of effort restoring our vintage Jeol JSM6100, we are finally up and running.
Looking for suggestions and/or recommendations on methods to mount and image elemental metal powders (1 to 20 micron range particle size). We are interested in imaging for geometry, particle size, and distribution. Currently we are using carbon adhesive tape (6mm X 20mm), either packing the powder on to the circular tape dot or dabbing the dot onto a powder sample resting on the lab bench. Does anyone have another more consistent and reliable technique that might help to assure more constant powder volumes and spreading about the tape dot?
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Name: M. A. Kirk / M. Li
Organization: Argonne National Laboratory / Nuclear Engineering Division
Title-Subject: PostDoc Position at the ANL IVEM/Tandem Facility
A postdoctoral position within the Nuclear Engineering Divison of Argonne National Laboratory is now open to work with Drs. M. A. Kirk and Meimei Li. The role of this position is to:
* Assist users of the IVEM-Tandem Facility in operation of the Hitachi H-9000 transmission electron microscope and in defect microscopy techniques for in situ ion irradiation experiments.
* Perform independent research in area of ion irradiation of materials, especially utilizing discretionary time on the IVEM-Tandem Facility.
*Considerable knowledge and expertise in Transmission Electron Microscopy (TEM) of materials is expected. Some knowledge in radiation effects in materials is desirable.
Detailed information about the ANL IVEM/Tandem facility can be found at the following URL
http://www.msd.anl.gov/groups/emc/ivem.php
----------------------------------------------------------------------- Interested candidates should visit the following URL for further information and to submit an application for the position.
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I recently got back from MMC 2014 in the UK (microscopy convention) and I was truly surprised that all the SEM manufactures still do not allow users to calibrate the scale bar on the new SEMS. The responses were all the same "our product IS calibrated in the factory, there is no need to mess with it" or "if you want to calibrate then why don't you run a standard in parallel?". When I push the reps most confess that the calibration is done at best on a ~2000l/mm grating which on our SEM does not equal good high mag measurements 400kX+. I am also amazed that most companies also do not correct for slow scan drift in the pixel so seeing as there is never a vertical scale bar in the images (well not ours) how can you believe the measurements?
I am not sure if it just me or my experience with older SEMs but I am sure there are many fields where a user might want to have independent calibration? I know this has been much discussed in the past but with digital recording we seem to have lost respect for calibration (perhaps this is just my jaded view?)
In our center we use a third party imaging system which is calibrated by ourselves against standards of our own making and verified with commercial standards and we get very good accuracy and reproducibility (we think). We account for all changes in beam and vacuum state. It certainly agrees with the TEM, XRD and other techniques we might use. The same can not be said for trusting the machine scalebars.We have very old machines. I am right in not trusting new machines as much as the old ones?
I would be interested to hear what peoples experiences are with this mag calibration issue especially in high mag. Has anyone found a way of doing this within a modern SEM? What standards do you use? Do you think the standards are good enough?
I also wanted to see if anyone would be interested in a little global test. We make LED devices in house so measurement for us is critical. I would like to propose that I send out to anyone interested a small piece of a bulk wafer (the same one!) with a pin array on it with a set size, shape, spacing. The aim would be to take pictures at set mags eg 10, 100, 250, 500kx and then make measurements based on your own lab method. You could then return the data to me along with the make and model of the SEM and your method of imaging/calibration. I would then collate and circulate the anonymised data to the list. This is not about the age of the SEM, the max mag, the type of source or even the lab budget I am just really interested in seeing what the real variation is out there.
If you have an interest in this and perhaps would like to participate in my little test (I would welcome instrument manufacturers to take part). If you want to reply to me on or off list any responses, opinions will be kept anonymous if requested.
Thanks for reading John
==============================Original Headers============================== 8, 26 -- From john.mitchels-at-gmail.com Sat Jul 19 15:41:08 2014 8, 26 -- Received: from mail-vc0-f177.google.com (mail-vc0-f177.google.com [209.85.220.177]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6JKf8rD019858 8, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Jul 2014 15:41:08 -0500 8, 26 -- Received: by mail-vc0-f177.google.com with SMTP id hy4so9490759vcb.22 8, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Jul 2014 13:41:07 -0700 (PDT) 8, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 26 -- d=gmail.com; s=20120113; 8, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 8, 26 -- bh=9YHpR0DPRB8J6Dd+kpAXKdd9NhBdW5O39JzQvvy5INE=; 8, 26 -- b=xagmZ+qTmq7Ipn+7M01D/ZsQbQ+VaykucBlaIzBhgQtoqa8qhWLYgs7bRAiO/jFYA9 8, 26 -- S4yCYn1HQRsZLxF2azG9X8zCgT9irJmCUfH0QDolcC1lL6KGmpzgLODBt347vwuTesIM 8, 26 -- b4iBS+RnyWRvrkkPXApt6XZbhKiugAnVK5t7UDHT/F7vbidQ9IQfIVBMxISoQtHBd6TB 8, 26 -- i0TwMhk/TnMPXyjjk2bELntS4592QJzkRoLZn6V8yjqwKEG0Q3+20qJkEYBgIaH/pKks 8, 26 -- wcDRQuYddInBtkfM2RwwuFUqroLgHW+t5Wd2c9kheEFXDckU+MwFn6GzBZfJ2CVTypsW 8, 26 -- mj6A== 8, 26 -- MIME-Version: 1.0 8, 26 -- X-Received: by 10.52.27.208 with SMTP id v16mr14322544vdg.21.1405802467583; 8, 26 -- Sat, 19 Jul 2014 13:41:07 -0700 (PDT) 8, 26 -- Received: by 10.220.6.136 with HTTP; Sat, 19 Jul 2014 13:41:07 -0700 (PDT) 8, 26 -- Date: Sat, 19 Jul 2014 21:41:07 +0100 8, 26 -- Message-ID: {CAHX7yTRHGeXTBBkouWb=Mk-Xr4DFLqMdGk3hFJzcb+iwCrapCA-at-mail.gmail.com} 8, 26 -- Subject: Mag calibration variation with SEMs, proposed: a little test 8, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 8, 26 -- To: Microscopy-at-microscopy.com 8, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
From medications_best9-at-ftninc.com Sun Jul 20 16:50:17 2014 Return-Path: {medications_best9-at-ftninc.com} Received: from mail1.ftninc.com ([92.47.129.55]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6KLoDTV014843 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 20 Jul 2014 16:50:14 -0500 Message-Id: {201407202150.s6KLoDTV014843-at-ns.microscopy.com}
John:
My mentor at HP, Nancy Phillips always had us check the Mag Cal on our many SEMs every quarter. We used an MRS 5 at that time, NIST traceable. If we found a measurement of the MRS 5 that deviated more than 5% we let FEI know and they (Jeff Cohen) would come change the Calibration for us! It was nice as we had an FEI service Engineer, Jeff, onsite. We really never saw deviations above 5% ever, a testament to Jeff's ability.
Now that I am at Oregon State, I have implemented a similar policy. I purchased an MRS 6, at $5k they are not cheap, and then I check all our SEMs at a random selection of Magnifications, voltages, detectors, ... We then update an excel spreadsheet that is shared between my coworker and I on a google drive. I have not seen deviation above 5%. Again, testament to our FEI guys: Bob Johnson and Frank. Just today I checked our Nova NanoSEM at 5kx, 10kx using the ETD and 100kx with the TLD in immersion. Deviations of the MRS 6 pitch, were well below 5%.
I would certainly participate in an inter lab comparison with your LED. I did one of these with the ANSI TAG group on gold nanoparticles a few years back and it was fun and educational.
Cheers,
Pete Eschbach Oregon State University Electron Microscope Facility Director 541 737 5645
-----Original Message----- X-from: john.mitchels-at-gmail.com [mailto:john.mitchels-at-gmail.com] Sent: Saturday, July 19, 2014 2:13 PM To: Eschbach, Peter
Dear listers
I recently got back from MMC 2014 in the UK (microscopy convention) and I was truly surprised that all the SEM manufactures still do not allow users to calibrate the scale bar on the new SEMS. The responses were all the same "our product IS calibrated in the factory, there is no need to mess with it" or "if you want to calibrate then why don't you run a standard in parallel?". When I push the reps most confess that the calibration is done at best on a ~2000l/mm grating which on our SEM does not equal good high mag measurements 400kX+. I am also amazed that most companies also do not correct for slow scan drift in the pixel so seeing as there is never a vertical scale bar in the images (well not ours) how can you believe the measurements?
I am not sure if it just me or my experience with older SEMs but I am sure there are many fields where a user might want to have independent calibration? I know this has been much discussed in the past but with digital recording we seem to have lost respect for calibration (perhaps this is just my jaded view?)
In our center we use a third party imaging system which is calibrated by ourselves against standards of our own making and verified with commercial standards and we get very good accuracy and reproducibility (we think). We account for all changes in beam and vacuum state. It certainly agrees with the TEM, XRD and other techniques we might use. The same can not be said for trusting the machine scalebars.We have very old machines. I am right in not trusting new machines as much as the old ones?
I would be interested to hear what peoples experiences are with this mag calibration issue especially in high mag. Has anyone found a way of doing this within a modern SEM? What standards do you use? Do you think the standards are good enough?
I also wanted to see if anyone would be interested in a little global test. We make LED devices in house so measurement for us is critical. I would like to propose that I send out to anyone interested a small piece of a bulk wafer (the same one!) with a pin array on it with a set size, shape, spacing. The aim would be to take pictures at set mags eg 10, 100, 250, 500kx and then make measurements based on your own lab method. You could then return the data to me along with the make and model of the SEM and your method of imaging/calibration. I would then collate and circulate the anonymised data to the list. This is not about the age of the SEM, the max mag, the type of source or even the lab budget I am just really interested in seeing what the real variation is out there.
If you have an interest in this and perhaps would like to participate in my little test (I would welcome instrument manufacturers to take part). If you want to reply to me on or off list any responses, opinions will be kept anonymous if requested.
Thanks for reading John
==============================Original Headers============================== 8, 26 -- From john.mitchels-at-gmail.com Sat Jul 19 15:41:08 2014 8, 26 -- Received: from mail-vc0-f177.google.com (mail-vc0-f177.google.com [209.85.220.177]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6JKf8rD019858 8, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Jul 2014 15:41:08 -0500 8, 26 -- Received: by mail-vc0-f177.google.com with SMTP id hy4so9490759vcb.22 8, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Jul 2014 13:41:07 -0700 (PDT) 8, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 26 -- d=gmail.com; s=20120113; 8, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 8, 26 -- bh=9YHpR0DPRB8J6Dd+kpAXKdd9NhBdW5O39JzQvvy5INE=; 8, 26 -- b=xagmZ+qTmq7Ipn+7M01D/ZsQbQ+VaykucBlaIzBhgQtoqa8qhWLYgs7bRAiO/jFYA9 8, 26 -- S4yCYn1HQRsZLxF2azG9X8zCgT9irJmCUfH0QDolcC1lL6KGmpzgLODBt347vwuTesIM 8, 26 -- b4iBS+RnyWRvrkkPXApt6XZbhKiugAnVK5t7UDHT/F7vbidQ9IQfIVBMxISoQtHBd6TB 8, 26 -- i0TwMhk/TnMPXyjjk2bELntS4592QJzkRoLZn6V8yjqwKEG0Q3+20qJkEYBgIaH/pKks 8, 26 -- wcDRQuYddInBtkfM2RwwuFUqroLgHW+t5Wd2c9kheEFXDckU+MwFn6GzBZfJ2CVTypsW 8, 26 -- mj6A== 8, 26 -- MIME-Version: 1.0 8, 26 -- X-Received: by 10.52.27.208 with SMTP id v16mr14322544vdg.21.1405802467583; 8, 26 -- Sat, 19 Jul 2014 13:41:07 -0700 (PDT) 8, 26 -- Received: by 10.220.6.136 with HTTP; Sat, 19 Jul 2014 13:41:07 -0700 (PDT) 8, 26 -- Date: Sat, 19 Jul 2014 21:41:07 +0100 8, 26 -- Message-ID: {CAHX7yTRHGeXTBBkouWb=Mk-Xr4DFLqMdGk3hFJzcb+iwCrapCA-at-mail.gmail.com} 8, 26 -- Subject: Mag calibration variation with SEMs, proposed: a little test 8, 26 -- From: John Mitchels {john.mitchels-at-gmail.com} 8, 26 -- To: Microscopy-at-microscopy.com 8, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 40 -- From Peter.Eschbach-at-oregonstate.edu Mon Jul 21 13:55:45 2014 20, 40 -- Received: from smtp1.nws.oregonstate.edu (smtp1.nws.oregonstate.edu [128.193.15.47]) 20, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6LItjaR005004 20, 40 -- for {Microscopy-at-Microscopy.com} ; Mon, 21 Jul 2014 13:55:45 -0500 20, 40 -- Received: from localhost (localhost [127.0.0.1]) 20, 40 -- by smtp1.nws.oregonstate.edu (Postfix) with ESMTP id 90CFE342202; 20, 40 -- Mon, 21 Jul 2014 11:55:44 -0700 (PDT) 20, 40 -- X-Virus-Scanned: Debian amavisd-new at relay1.nws.oregonstate.edu 20, 40 -- Received: from smtp1.nws.oregonstate.edu ([127.0.0.1]) 20, 40 -- by localhost (smtp1.nws.oregonstate.edu [127.0.0.1]) (amavisd-new, port 10024) 20, 40 -- with ESMTP id rp_zdxPCMvww; Mon, 21 Jul 2014 11:55:44 -0700 (PDT) 20, 40 -- Received: from EX1.oregonstate.edu (ex1.oregonstate.edu [128.193.7.76]) 20, 40 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 20, 40 -- (No client certificate requested) 20, 40 -- by smtp1.nws.oregonstate.edu (Postfix) with ESMTPS id 786693421FC; 20, 40 -- Mon, 21 Jul 2014 11:55:44 -0700 (PDT) 20, 40 -- Received: from EX3.oregonstate.edu ([169.254.3.194]) by EX1.oregonstate.edu 20, 40 -- ([169.254.1.103]) with mapi id 14.03.0158.001; Mon, 21 Jul 2014 11:55:44 20, 40 -- -0700 20, 40 -- From: "Eschbach, Peter" {Peter.Eschbach-at-oregonstate.edu} 20, 40 -- To: "john.mitchels-at-gmail.com" {john.mitchels-at-gmail.com} 20, 40 -- CC: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 20, 40 -- Subject: RE: [Microscopy] Mag calibration variation with SEMs, proposed: a 20, 40 -- little test 20, 40 -- Thread-Topic: [Microscopy] Mag calibration variation with SEMs, proposed: a 20, 40 -- little test 20, 40 -- Thread-Index: AQHPo5Y1FeL+suGwMUq6EDiJ2qBwM5uq4HDw 20, 40 -- Date: Mon, 21 Jul 2014 18:55:43 +0000 20, 40 -- Message-ID: {D237C7ECC2E1574C9D6661AADAB3BD50088774E1-at-EX3.oregonstate.edu} 20, 40 -- References: {201407192112.s6JLCr0G006536-at-ns.microscopy.com} 20, 40 -- In-Reply-To: {201407192112.s6JLCr0G006536-at-ns.microscopy.com} 20, 40 -- Accept-Language: en-US 20, 40 -- Content-Language: en-US 20, 40 -- X-MS-Has-Attach: 20, 40 -- X-MS-TNEF-Correlator: 20, 40 -- x-originating-ip: [128.193.7.75] 20, 40 -- Content-Type: text/plain; charset="us-ascii" 20, 40 -- MIME-Version: 1.0 20, 40 -- Content-Transfer-Encoding: 8bit 20, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6LItjaR005004 ==============================End of - Headers==============================
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Message: Hi All: I am looking for recommendations of particle counting programs that function reliably and require minimal interaction to obtain sizing information on particles in TEM images. If you have an estimate on the program cost that would also be helpful. Programs I have used in the past, seem to be fussy and difficult to use, especially when used on particles with limited contrast. Thanks in advance, Sandra
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jul 21 19:05:06 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6M056sA028159 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 21 Jul 2014 19:05:06 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id B847C731B9A 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 21 Jul 2014 19:05:06 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id QsRi8ywoFIeC for {microscopy-at-microscopy.com} ; 12, 28 -- Mon, 21 Jul 2014 19:04:57 -0500 (CDT) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id 31E25731B8C 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 21 Jul 2014 19:04:57 -0500 (CDT) 12, 28 -- Message-ID: {53CDAAA8.8010804-at-microscopy.com} 12, 28 -- Date: Mon, 21 Jul 2014 19:04:56 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:TEM-Particle counting software 12, 28 -- References: {201407211549.s6LFnWD4002146-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201407211549.s6LFnWD4002146-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201407211549.s6LFnWD4002146-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello John Happy to participate to your test. My impression from my Jeol JSM5600LV is that a variation of at least 5% in magnification scale may occur within two measurements on the same sample with the same microscope settings, provided that the microscope was shut down and restarted between these two measurements. Also, I have an external scan generation device (DISS5 from "pointelectronic") that is also calibrated and can be tested for accuracy. With best wishes yorgos
----- Original Message ----- X-from: {john.mitchels-at-gmail.com} To: {eikonika-at-otenet.gr} Sent: Saturday, July 19, 2014 11:56 PM
On 22.07.2014 02:34, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: sk-at-tem-analysis.com () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both sk-at-tem-analysis.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: sk-at-tem-analysis.com } Name: Sandra Keller } } Organization: TA } } Title-Subject: [Filtered] TEM-Particle counting software } } Message: Hi All: } I am looking for recommendations of particle counting programs that function reliably and require } minimal interaction to obtain sizing information on particles in TEM images. If you have an } estimate on the program cost that would also be helpful. Programs I have used in the past, seem to } be fussy and difficult to use, especially when used on particles with limited contrast. } Thanks in advance, } Sandra } } Login Host: 71.11.236.56 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } Hi Sandra, Look up the page http://www.jmicrovision.com/. Maybe this piece of soft would fulfill your expectations. All the best
-- Jean-Michel Jaquet, PhD Geomicrobiology Group Section of Earth and Environmental Sciences University of Geneva 13 rue des Maraîchers CH-1211 Geneva 3 Switzerland http://cms.unige.ch/sciences/terre/research/Groups/limnogeology/limno.php
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Message: Sorry, I should entitled my post: TEM-particle measurement software rather than TEM-Particle counting software. Again, I am looking for recommendations of particle measurement programs that function reliably and require minimal interaction to obtain sizing information on particles in TEM images. If you have an estimate on the program cost that would also be helpful. Programs that I have used in the past, seem to be fussy and difficult to use, especially when used on particles with limited contrast. Thanks in advance, Sandra
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==============================Original Headers============================== 10, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Jul 22 08:17:23 2014 10, 28 -- Received: from znl.com ([206.69.208.20]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6MDHNKf031645 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Jul 2014 08:17:23 -0500 10, 28 -- Received: from localhost (localhost [127.0.0.1]) 10, 28 -- by znl.com (Postfix) with ESMTP id 65B80732225 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Jul 2014 08:17:23 -0500 (CDT) 10, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 28 -- Received: from znl.com ([127.0.0.1]) 10, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id dKNe1990oZuA for {microscopy-at-microscopy.com} ; 10, 28 -- Tue, 22 Jul 2014 08:17:16 -0500 (CDT) 10, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 10, 28 -- by znl.com (Postfix) with ESMTPA id D8ABC732213 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 22 Jul 2014 08:17:16 -0500 (CDT) 10, 28 -- Message-ID: {53CE645C.5010708-at-microscopy.com} 10, 28 -- Date: Tue, 22 Jul 2014 08:17:16 -0500 10, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 10, 28 -- MIME-Version: 1.0 10, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 28 -- Subject: viaWWW:TEM-Particle measurement software-corrected 10, 28 -- References: {201407220203.s6M23lKu016395-at-ns.microscopy.com} 10, 28 -- In-Reply-To: {201407220203.s6M23lKu016395-at-ns.microscopy.com} 10, 28 -- X-Forwarded-Message-Id: {201407220203.s6M23lKu016395-at-ns.microscopy.com} 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Yorgos, That can (will) definitely happen if you are not, in some way, normalizing your hysteresis. Depending upon the microscope, there are different ways to normalize. Some involve reversing the lens currents for a bit, while others simply turn off the lens currents. Either way, your lenses can be considered "normalized" if you use what ever routine is appropriate for your scope twice in a row and the image doesn't need refocusing at 1000X.
As an experiment, focus at a long working distance and collect an image of something that you can measure. Run your focus control all the way up (shortest working distance), then back down to focus and collect another image. I think you'll find the actual mags may be the same but, with most instruments, the readouts will be quite different because in the second image the instrument thinks the WD is much longer, so the WD multiplier is less than in the first image.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr] Sent: Monday, July 21, 2014 10:27 PM To: kenconverse-at-qualityimages.biz
Hello John Happy to participate to your test. My impression from my Jeol JSM5600LV is that a variation of at least 5% in magnification scale may occur within two measurements on the same sample with the same microscope settings, provided that the microscope was shut down and restarted between these two measurements. Also, I have an external scan generation device (DISS5 from "pointelectronic") that is also calibrated and can be tested for accuracy. With best wishes yorgos
----- Original Message ----- X-from: {john.mitchels-at-gmail.com} To: {eikonika-at-otenet.gr} Sent: Saturday, July 19, 2014 11:56 PM
We recently had the Gatan HAADF detector off of our JEOL 2100F. I measured inner and outer diameters and found that the angles in the manual at the specified camera length were not correct. So, in addition to going through the exercise of measuring the collection angles at different camera lengths for my GIF, I carefully measured the camera lengths at the HAADF position and calculated the inner and outer collection angles for the HAADF detector.
I work with a lot of different materials, from polymers and light element ceramics to heavy element alloys. My question is there a method to optimize the cameral length, i.e. collection angles for a particular material system when you know the elements? Should I base it on the EELS characteristic angles for the elements? If I do, use the K shells? If I want to maximize the contrast, should I try to exclude the lower Z characteristic angle from the detector?
Hi Scott, I did this task using NIST's Elastic32 program. This calculates the differential electron scattering cross section for elements; combined with a quick integration over the correct angles, relative counts can be determined. Regards, Larry Scipioni
-----Original Message----- X-from: s.walck-at-comcast.net [mailto:s.walck-at-comcast.net] Sent: Thursday, July 24, 2014 9:07 AM To: LES-at-ZSGENETICS.COM
We recently had the Gatan HAADF detector off of our JEOL 2100F. I measured inner and outer diameters and found that the angles in the manual at the specified camera length were not correct. So, in addition to going through the exercise of measuring the collection angles at different camera lengths for my GIF, I carefully measured the camera lengths at the HAADF position and calculated the inner and outer collection angles for the HAADF detector.
I work with a lot of different materials, from polymers and light element ceramics to heavy element alloys. My question is there a method to optimize the cameral length, i.e. collection angles for a particular material system when you know the elements? Should I base it on the EELS characteristic angles for the elements? If I do, use the K shells? If I want to maximize the contrast, should I try to exclude the lower Z characteristic angle from the detector?
From xihleni-at-mail2moon.com Fri Jul 25 04:15:38 2014 Return-Path: {xihleni-at-mail2moon.com} Received: from mail3.mail2moon.com ([79.105.175.33]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6P9FZPn006565 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 25 Jul 2014 04:15:36 -0500 Message-ID: {E7093BAF.0720895-at-mail2moon.com}
X-from: tina.williams-at-ars.usda.gov ()
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Email: tina.williams-at-ars.usda.gov Name: Tina Williams
Organization: USDA
Title-Subject: [Filtered] 1.CryoJane tape transfer 2.Recommendations for confocal system
Message: Hi, Two questions:
1. Does anyone know of CryoJane Tape transfer users in the San Francisco Bay Area or nearby, who would be willing to demonstrate their unit? We are wondering if it will work with our current cryostat CM3000, and plant tissues?
2. Does anyone have recommendations for models/components on a confocal system for imaging plant tissues? We are considering purchasing a confocal system.
Thank you, Tina
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Message: Sorry, I should entitled my post: TEM-particle measurement software rather than TEM-Particle counting software. Again, I am looking for recommendations of particle measurement programs that function reliably and require minimal interaction to obtain sizing information on particles in TEM images. If you have an estimate on the program cost that would also be helpful. Programs that I have used in the past, seem to be fussy and difficult to use, especially when used on particles with limited contrast. Thanks in advance, Sandra
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Title-Subject: [Filtered] Vacuum Leak in JEOL 2100 TEM Holder
Message: I am having a vacuum leak with a TEM specimen holder but cannot pinpoint it. The holder behaves OK in a vacuum pumping station, reaching ~7 x 10^-6 Torr in about 30 minutes. After this, I transfer to the TEM, where it pumps down at the first stage of pumping in about 5 minutes. However, after this, when I do the rotation of the holder, just before the full insertion, the vacuum in the column becomes way too high. In particular, the vacuum ion gage, located in the lower left panel of the microscope, which reads the vacuum at the pole piece, goes out of scale. The vacuum readout for the first stage of pumping remains in a good range though. Where the vacuum leak may be? Is there a way to pinpoint which of the two o-rings is suspect (I've exchanged them and greased them but the leak is still there).
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA-ERRC
Title-Subject: [Filtered] agarose pellet stain
Message: I'm suspending small pectin beads in agarose for ease of handling through the dehydration/embedding steps.
When I embed in LR white the 'pellet' becomes transparent.
Is there anything I could add to the agarose to give it optical density without adding artifacts in the thin section? I presume the ethanol dehydration steps will dissolve out most dyes i could add...
thanks
Joe
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X-from: christinaheard83-at-gmail.com ()
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Email: christinaheard83-at-gmail.com Name: C. Heard
Organization: Pathlogic
Title-Subject: [Filtered] Grainy TEM images
Message: I am a new EM tech at a pathology lab specific to renal tissues. My images on the TEM have been grainy and the resolution is suffering. I have adjusted my cutting angles, slowed the ultra microtome speed to 1mm/s, and I post stain with UA and LC for 5 minutes each. If anyone out there has an idea of a solution to the grainy images, it would be greatly appreciated.
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==============================Original Headers============================== 15, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jul 28 07:35:30 2014 15, 28 -- Received: from znl.com ([206.69.208.20]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6SCZTbM022714 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 07:35:30 -0500 15, 28 -- Received: from localhost (localhost [127.0.0.1]) 15, 28 -- by znl.com (Postfix) with ESMTP id CD7C47371EB 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 07:35:29 -0500 (CDT) 15, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 28 -- Received: from znl.com ([127.0.0.1]) 15, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 28 -- with ESMTP id 1G336Sqg8oL8 for {microscopy-at-microscopy.com} ; 15, 28 -- Mon, 28 Jul 2014 07:35:23 -0500 (CDT) 15, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 15, 28 -- by znl.com (Postfix) with ESMTPA id 621047371D9 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 07:35:23 -0500 (CDT) 15, 28 -- Message-ID: {53D6438B.40201-at-microscopy.com} 15, 28 -- Date: Mon, 28 Jul 2014 07:35:23 -0500 15, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 15, 28 -- MIME-Version: 1.0 15, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 28 -- Subject: viaWWW:Grainy TEM images 15, 28 -- References: {201407280535.s6S5ZUX5018366-at-ns.microscopy.com} 15, 28 -- In-Reply-To: {201407280535.s6S5ZUX5018366-at-ns.microscopy.com} 15, 28 -- X-Forwarded-Message-Id: {201407280535.s6S5ZUX5018366-at-ns.microscopy.com} 15, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have a researcher who would like access to an SEM that can do an automated scan of particles and automated EDS of the particles. Anyone out there close to Omaha, Nebraska? This researcher is a chemist working on aerosol particles. All replies appreciated.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Mon Jul 28 09:08:03 2014 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6SE83Lc010613 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 09:08:03 -0500 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id 6A9945A9EBB 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 09:03:58 -0500 (CDT) 7, 37 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 7EF8C5A9EBA 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 09:03:57 -0500 (CDT) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 37 -- 14.03.0181.006; Mon, 28 Jul 2014 09:08:00 -0500 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Automated SEM and Particle analysis 7, 37 -- Thread-Topic: Automated SEM and Particle analysis 7, 37 -- Thread-Index: Ac+qbJZ5vhsUP/ypTf26tuSQt3EkUw== 7, 37 -- Date: Mon, 28 Jul 2014 14:07:59 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0746AB7B9-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: adb18f61-3ab9-4942-b936-d0258e3da7c8 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: ff53635f-1f70-4ea1-b767-de139521ea53 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6SE83Lc010613 ==============================End of - Headers==============================
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Best primary fixative for post-embedding immunogold or FluoroNanogold(TM)?
Message: Hello All, What is the optimum buffer to use for a PFA/very light GTA fixative for immunoTEM on thin sections? Is sodium cacodylate contraindicated wrt. antigen recognition?
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jul 28 10:25:52 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6SFPpWp031192 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 10:25:51 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id D82877373B3 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 10:25:51 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id 0dcmSCzpBs9W for {microscopy-at-microscopy.com} ; 12, 28 -- Mon, 28 Jul 2014 10:25:47 -0500 (CDT) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id F114E7373A6 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 10:25:46 -0500 (CDT) 12, 28 -- Message-ID: {53D66B7A.60303-at-microscopy.com} 12, 28 -- Date: Mon, 28 Jul 2014 10:25:46 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Best primary fixative for post-embedding immunogold or FluoroNanogold(TM)? 12, 28 -- References: {201407281521.s6SFLCdn031087-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201407281521.s6SFLCdn031087-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201407281521.s6SFLCdn031087-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
} Best primary fixative for post-embedding } immunogold or FluoroNanogold(TM)?
the best primary fixative is definitely high-pressure freezing, freeze-substitution (omitting or reducing OsO4), and then any suitable follow-up technique. Following HPF/FS, Epon is not contra-indicated to post-embedding immunogold detection (even not low amounts of OsO4!), although quite many people believe it is. LR-White or Gold or Lowicryls might be better - to some degree, for a certain number and kind of specimen - , but this is not always the case.
} What is the optimum buffer to use for a PFA/very light GTA fixative for } immunoTEM on thin sections? I would go for HEPES or MES or anything similar, these buffers are superior to most others, in most cases, for most applications. Do not forget that it is not only the buffer which matters; the other ingredients need to be optimized, in concentration and in composition, for your tissue under investigation. PBS or TBS or whatsoever is not always the best - in fact, it might be among the worst.
} Is sodium cacodylate contraindicated wrt. antigen recognition? Why still using caco if anything better is around? it is not only the disposal of caco, which may cause problems. kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: http://www.imc2014.com/ 18th IMC 2014 in Prague, CZ http://www.mc2015.de DGE - Microscopy Conference 2015, Sept 6-11, Göttingen
==============================Original Headers============================== 7, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Mon Jul 28 11:32:32 2014 7, 25 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6SGWVwR018790 7, 25 -- for {Microscopy-at-Microscopy.com} ; Mon, 28 Jul 2014 11:32:32 -0500 7, 25 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 7, 25 -- by localhost (Postfix) with SMTP id EFA6037ED3 7, 25 -- for {Microscopy-at-Microscopy.com} ; Mon, 28 Jul 2014 18:32:26 +0200 (CEST) 7, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 7, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 25 -- (Client CN "gwsmtp.uni-regensburg.de", Issuer "Uni Regensburg CA - G01" (not verified)) 7, 25 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTPS id C04F95F7 7, 25 -- for {Microscopy-at-Microscopy.com} ; Mon, 28 Jul 2014 18:32:26 +0200 (CEST) 7, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 7, 25 -- with Novell_GroupWise; Mon, 28 Jul 2014 18:32:28 +0200 7, 25 -- Message-Id: {53D69739020000540004C515-at-gwsmtp1.uni-regensburg.de} 7, 25 -- X-Mailer: Novell GroupWise Internet Agent 12.0.3 7, 25 -- Date: Mon, 28 Jul 2014 18:32:25 +0200 7, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 7, 25 -- To: {Microscopy-at-Microscopy.com} 7, 25 -- Cc: {vakimler-at-med.wayne.edu} 7, 25 -- Subject: primary fixative immunoTEM 7, 25 -- Mime-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=UTF-8 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- Content-Disposition: inline ==============================End of - Headers==============================
Vickie, For chemically stabilizing material, I prefer 2% Pf 0.2% Ga in PHEM buffer. This can be followed with light tannic acid then UA before embedding. This should be done cold and kept cold throughout either LR White or HM20 infiltration. If possible HPF live or lightly fixed material then AFS with a Ga UA or a Ga UA OsO4 combo (0.2 0.2 0.1%). Then infiltrate at -50C with HM20 (a freeze substitution machine helps) Good luck, Michael Delannoy
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Monday, July 28, 2014 11:48 AM To: mdelann1-at-jhmi.edu
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Best primary fixative for post-embedding immunogold or FluoroNanogold(TM)?
Message: Hello All, What is the optimum buffer to use for a PFA/very light GTA fixative for immunoTEM on thin sections? Is sodium cacodylate contraindicated wrt. antigen recognition?
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Jul 28 10:25:52 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6SFPpWp031192 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 10:25:51 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id D82877373B3 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 10:25:51 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id 0dcmSCzpBs9W for {microscopy-at-microscopy.com} ; 12, 28 -- Mon, 28 Jul 2014 10:25:47 -0500 (CDT) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id F114E7373A6 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 10:25:46 -0500 (CDT) 12, 28 -- Message-ID: {53D66B7A.60303-at-microscopy.com} 12, 28 -- Date: Mon, 28 Jul 2014 10:25:46 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Best primary fixative for post-embedding immunogold or FluoroNanogold(TM)? 12, 28 -- References: {201407281521.s6SFLCdn031087-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201407281521.s6SFLCdn031087-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201407281521.s6SFLCdn031087-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have an older BSD attached to the bottom of the lens on an older XL30CP SEM donated to us from a crime lab where EDS was done for ballistics. Pulling the BSD off, one half of the detector has a dried fim on the Si diode, I tried to remove using 200proof ethanol even sonicating for 1-2 minutes without any luck.
I then put the BSD in the SEM and did EDS on the surface at 15kv and got the following composition: Al 30% F 39% O 17% C 5.5%
The rest of the comp was Si and Copper from the holder
Any ideas on cleaning ?
I have no details on how the BSD was used other than it was attached, under vac when we picked up the microscope
Thank you
Fred Hayes AMCaT Manager UC Davis Chem Eng Mat Sci
==============================Original Headers============================== 8, 26 -- From fahayes-at-ucdavis.edu Mon Jul 28 16:04:02 2014 8, 26 -- Received: from msa1.ucdavis.edu (msa1.ucdavis.edu [128.120.32.181]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6SL41nB030722 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 16:04:01 -0500 8, 26 -- Received: from CHMSK011201 (chms-3010a.engr.ucdavis.edu [169.237.64.45]) 8, 26 -- (authenticated bits=0) 8, 26 -- by msa1.ucdavis.edu (8.14.4/8.14.5/it-oel6-mimedefang-smtp-1.9) with ESMTP id s6SL3xLF021550 8, 26 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 14:03:59 -0700 8, 26 -- From: "Fred Hayes" {fahayes-at-ucdavis.edu} 8, 26 -- To: {Microscopy-at-microscopy.com} 8, 26 -- References: 8, 26 -- In-Reply-To: 8, 26 -- Subject: FW: removing film on XL30CP SEM BSD 8, 26 -- Date: Mon, 28 Jul 2014 14:03:59 -0700 8, 26 -- Message-ID: {002a01cfaaa7$73e372e0$5baa58a0$-at-ucdavis.edu} 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Content-Transfer-Encoding: 7bit 8, 26 -- X-Mailer: Microsoft Outlook 15.0 8, 26 -- Thread-Index: Ac+qocZ1b3+uZjpUROmByk072eYKiQABZODw 8, 26 -- Content-Language: en-us 8, 26 -- X-Virus-Scanned: clamav-milter 0.98.1 at msa1 8, 26 -- X-Virus-Status: Clean 8, 26 -- X-Scanned-By: MIMEDefang 2.74 ==============================End of - Headers==============================
Fred, If you think there is an adhesive involved, try hexane (gasoline) to soften it.
Not at all sure why someone would put a film directly on the diode.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: fahayes-at-ucdavis.edu [mailto:fahayes-at-ucdavis.edu] Sent: Monday, July 28, 2014 5:11 PM To: kenconverse-at-qualityimages.biz
I have an older BSD attached to the bottom of the lens on an older XL30CP SEM donated to us from a crime lab where EDS was done for ballistics. Pulling the BSD off, one half of the detector has a dried fim on the Si diode, I tried to remove using 200proof ethanol even sonicating for 1-2 minutes without any luck.
I then put the BSD in the SEM and did EDS on the surface at 15kv and got the following composition: Al 30% F 39% O 17% C 5.5%
The rest of the comp was Si and Copper from the holder
Any ideas on cleaning ?
I have no details on how the BSD was used other than it was attached, under vac when we picked up the microscope
Thank you
Fred Hayes AMCaT Manager UC Davis Chem Eng Mat Sci
==============================Original Headers============================== 8, 26 -- From fahayes-at-ucdavis.edu Mon Jul 28 16:04:02 2014 8, 26 -- Received: from msa1.ucdavis.edu (msa1.ucdavis.edu [128.120.32.181]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6SL41nB030722 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 16:04:01 -0500 8, 26 -- Received: from CHMSK011201 (chms-3010a.engr.ucdavis.edu [169.237.64.45]) 8, 26 -- (authenticated bits=0) 8, 26 -- by msa1.ucdavis.edu (8.14.4/8.14.5/it-oel6-mimedefang-smtp-1.9) with ESMTP id s6SL3xLF021550 8, 26 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jul 2014 14:03:59 -0700 8, 26 -- From: "Fred Hayes" {fahayes-at-ucdavis.edu} 8, 26 -- To: {Microscopy-at-microscopy.com} 8, 26 -- References: 8, 26 -- In-Reply-To: 8, 26 -- Subject: FW: removing film on XL30CP SEM BSD 8, 26 -- Date: Mon, 28 Jul 2014 14:03:59 -0700 8, 26 -- Message-ID: {002a01cfaaa7$73e372e0$5baa58a0$-at-ucdavis.edu} 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Content-Transfer-Encoding: 7bit 8, 26 -- X-Mailer: Microsoft Outlook 15.0 8, 26 -- Thread-Index: Ac+qocZ1b3+uZjpUROmByk072eYKiQABZODw 8, 26 -- Content-Language: en-us 8, 26 -- X-Virus-Scanned: clamav-milter 0.98.1 at msa1 8, 26 -- X-Virus-Status: Clean 8, 26 -- X-Scanned-By: MIMEDefang 2.74 ==============================End of - Headers==============================
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==============================Original Headers============================== 21, 27 -- From kenconverse-at-qualityimages.biz Tue Jul 29 07:04:30 2014 21, 27 -- Received: from cdptpa-oedge-vip.email.rr.com (cdptpa-outbound-snat.email.rr.com [107.14.166.230]) 21, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6TC4TMj026819 21, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jul 2014 07:04:30 -0500 21, 27 -- Received: from [108.183.173.180] ([108.183.173.180:1632] helo=Ken) 21, 27 -- by cdptpa-oedge03 (envelope-from {kenconverse-at-qualityimages.biz} ) 21, 27 -- (ecelerity 3.5.0.35861 r(Momo-dev:tip)) with ESMTP 21, 27 -- id 83/B0-27317-DCD87D35; Tue, 29 Jul 2014 12:04:29 +0000 21, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 27 -- To: {fahayes-at-ucdavis.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 21, 27 -- Subject: RE: [Microscopy] FW: removing film on XL30CP SEM BSD 21, 27 -- Date: Tue, 29 Jul 2014 08:06:15 -0400 21, 27 -- Message-ID: {5D4D9CDF78944B58AA4F99DFB8CABC47-at-Ken} 21, 27 -- MIME-Version: 1.0 21, 27 -- Content-Type: text/plain; 21, 27 -- charset="us-ascii" 21, 27 -- X-Priority: 3 (Normal) 21, 27 -- X-MSMail-Priority: Normal 21, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 21, 27 -- In-Reply-To: {201407282111.s6SLBDKn001400-at-ns.microscopy.com} 21, 27 -- Importance: Normal 21, 27 -- Thread-Index: Ac+qqJSE8SeqU+3VRBuLUbD+HEYbzwAfITQg 21, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 21, 27 -- X-RR-Connecting-IP: 107.14.168.142:25 21, 27 -- X-Cloudmark-Score: 0 21, 27 -- Content-Transfer-Encoding: 8bit 21, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6TC4TMj026819 ==============================End of - Headers==============================
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Email: caromill-at-iupui.edu Name: Caroline A Miller
Organization: MSA
Title-Subject: [Filtered] M & M meeting 2014 Cryo FIG
Message: This is to let everyone know I have a few more spots available for lunch. The meeting will be held on, Tuesday, August 5th at 12:15, HM-Conference Room 7. This meeting will be to elect officers. Lunch provided. Please let me know if you will be attending! It is important to see how much interest there is in continuing this Focused interest group.
Thanks, Caroline Miller, Interim Leader Elect
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Proper sample preparation is perhaps the most important element in successful AFM imaging. Techniques for sample preparation in life sciences, material sciences, and other applications will be covered. This livestreaming seminar will be led by Peter Eaton Ph.D (Univ.Porto - Requimte) and Paul West Ph.D (AFMWorkshop, Inc). Attendees are welcome to ask questions and participate live via 'chat'.
To Register: http://www.ustream.tv/channel/Atomic-Force-Microscopes
Thank you, Pamela K. Stone AFMWorkshop, Inc. 1434 E. 33rd Street Signal Hill, CA 90755 www.afmworkshop.com
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From meds-discounted5-at-rr.com Wed Jul 30 12:01:51 2014 Return-Path: {meds-discounted5-at-rr.com} Received: from cpe-108-185-189-226.socal.res.rr.com (cpe-108-185-189-226.socal.res.rr.com [108.185.189.226]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6UH1ncG014105 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 30 Jul 2014 12:01:49 -0500 Message-Id: {201407301701.s6UH1ncG014105-at-ns.microscopy.com}
Would like to hear comments about the Leica ACE600 sputter coater from any users. Interested in quality of coating for HR-FESEM using conductive metal coatings, time it takes to reach proper vacuum, what vacuum do you achieve before starting the coating process, other pertinent comments. Offline comments are fine.
Thanks for your time.
Stephen McCartney Senior Research Associate ICTAS/NCFL 1991 Kraft Dr. Va Tech Blacksburg, VA 24061 USA
540-231-9765-office 540-553-6954-cell
http://www.ncfl.ictas.vt.edu/
==============================Original Headers============================== 12, 50 -- From stmccart-at-vt.edu Thu Jul 31 16:00:40 2014 12, 50 -- Received: from omr2.cc.vt.edu (omr2.cc.vt.edu [198.82.141.100]) 12, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s6VL0esZ002695 12, 50 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Jul 2014 16:00:40 -0500 12, 50 -- Received: from mr3.cc.vt.edu (mr3.cc.vt.edu [198.82.141.68] (may be forged)) 12, 50 -- by omr2.cc.vt.edu (8.14.4/8.14.4) with ESMTP id s6VL0eiX000939 12, 50 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Jul 2014 17:00:40 -0400 12, 50 -- Received: from mail-qa0-f49.google.com (mail-qa0-f49.google.com [209.85.216.49]) 12, 50 -- by mr3.cc.vt.edu (8.14.4/8.14.4) with ESMTP id s6VL0YQI012018 12, 50 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Jul 2014 17:00:39 -0400 12, 50 -- Received: by mail-qa0-f49.google.com with SMTP id dc16so2950778qab.22 12, 50 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Jul 2014 14:00:34 -0700 (PDT) 12, 50 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 50 -- d=1e100.net; s=20130820; 12, 50 -- h=x-gm-message-state:from:to:subject:date:message-id:mime-version 12, 50 -- :content-type:content-transfer-encoding:thread-index 12, 50 -- :content-language; 12, 50 -- bh=3IoJllSwsAyXXIRifl9e1Vdbnvvl763wfM4UV8p6UrM=; 12, 50 -- b=MtuCK2sQJavECW67fs9UdI9sqsJ6oSTwSKyw+RV7X6TGNCoVpt27J3p3dsKX9Ozdm2 12, 50 -- MQQNBW4qF60RcmQGM77vly8fsQSCDC1y5L3WfT4FoAKpNJXBMjOQndlhd0MEaKsRYrhY 12, 50 -- DD9WP4Ljz2Lo8e1iej5i6607QGA9q/NPdwokn68dmEYiaELMNQheZuozXo7kQBLYn4Ls 12, 50 -- DF4fdVHyXuKdtJ03WWFMxtoQDqtL87LKqoz3Z0spL86a8uaOIOy+2whPQomDYMzqI5Gi 12, 50 -- Me8lAGTU8Lhf348I10r+E8LodxOY7a3nTnccgTXZj3hEaeIIrCuBX5F6JoDpV8KSr/Pl 12, 50 -- RB5Q== 12, 50 -- X-Gm-Message-State: ALoCoQl4olVe7AE4+k4Km90UngvoRxQtXaz5Rjxyho/Bz4/JK1W5rn+X4aDEHEMUGjqOgcPYe25le8tc0O+H9kumvOWwuW93dkJ+zMU9ne+2mJAhG7Hs7azZXWD8TZabkzkpYJhaCVNtVzBoz3khCoXkJXruFiQvUA== 12, 50 -- X-Received: by 10.140.108.68 with SMTP id i62mr1294420qgf.56.1406840434572; 12, 50 -- Thu, 31 Jul 2014 14:00:34 -0700 (PDT) 12, 50 -- X-Received: by 10.140.108.68 with SMTP id i62mr1294410qgf.56.1406840434497; 12, 50 -- Thu, 31 Jul 2014 14:00:34 -0700 (PDT) 12, 50 -- Received: from d2zvmm91 ([2001:468:c80:2134:7827:4cf5:595d:ea74]) 12, 50 -- by mx.google.com with ESMTPSA id p20sm11384431qay.15.2014.07.31.14.00.33 12, 50 -- for {Microscopy-at-microscopy.com} 12, 50 -- (version=TLSv1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 12, 50 -- Thu, 31 Jul 2014 14:00:34 -0700 (PDT) 12, 50 -- From: "Stephen McCartney" {stmccart-at-vt.edu} 12, 50 -- To: {Microscopy-at-microscopy.com} 12, 50 -- Subject: Leica ACE600 sputter coater 12, 50 -- Date: Thu, 31 Jul 2014 17:00:33 -0400 12, 50 -- Message-ID: {024401cfad02$78419ee0$68c4dca0$-at-vt.edu} 12, 50 -- MIME-Version: 1.0 12, 50 -- Content-Type: text/plain; 12, 50 -- charset="iso-8859-1" 12, 50 -- X-Mailer: Microsoft Outlook 14.0 12, 50 -- Thread-Index: Ac+tAhkYgJIh/ZnfRfqw60nnOEsUCA== 12, 50 -- Content-Language: en-us 12, 50 -- X-Spam-Status: No, score=-0.7 required=5.0 tests=RCVD_IN_DNSWL_LOW 12, 50 -- autolearn=disabled version=3.3.1 12, 50 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr3.cc.vt.edu 12, 50 -- Content-Transfer-Encoding: 8bit 12, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s6VL0esZ002695 ==============================End of - Headers==============================
Any hints as to how to handle relocation into a new building, in a smallish area between the four main lift (elevator) shafts, and next to the heaviest traffic corridor from the main loading dock. Building will house 600+ scientists, etc. which gives an idea of the traffic. The architects, and presumably our corporate overlords, appear to have locked into the building design, and say the microscopes just have to go in this place.
My preferred option is to insist on external specialist consultants to design something that will elminate or at least minimise any vibration and electromagnetic interference. Any other suggestions?
thanks, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
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Message: We would like to stain proteoglycans in tissue with cupromeronic blue for EM studies. Does anyone know which companies are still selling cupromeronic blue?
There are many other stains used for staining proteoglycans such as Alican Blue, Toluidine Blue, Ruthenium Red, safranin O etc? Which one works best? Any recommendation would be appreciated.
Thank you
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I'm dealing with a forthcoming move myself, although not like this. Assuming you have to move, and can't stay where you are, then first, have the EM companies whose 'scope you have come in and do site surveys. When the site fails for both vibration and EMF, then insist on getting the antivibration and EMF shielding before the move.
Might even be cheaper to build a ground-level addition to the building with a proper, isolated foundation. Since this is an "addition" and not a change to the building design, that might be approved. Especially after the survey failures.
Phil
} Dear all, } } Any hints as to how to handle relocation into a new building, in a } smallish area between the four main lift (elevator) shafts, and next to } the heaviest traffic corridor from the main loading dock. Building will } house 600+ scientists, etc. which gives an idea of the traffic. The } architects, and presumably our corporate overlords, appear to have locked } into the building design, and say the microscopes just have to go in this } place. } } My preferred option is to insist on external specialist consultants to } design something that will elminate or at least minimise any vibration and } electromagnetic interference. Any other suggestions? } } thanks, } Rosemary } } Dr Rosemary White } CSIRO Plant Industry } GPO Box 1600 } Canberra, ACT 2601 } Australia } } T 61 2 6246 5475 } F 61 2 6246 5334 } E rosemary.white-at-csiro.au -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Fri Aug 1 07:10:23 2014 5, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s71CAN8F003463 5, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Aug 2014 07:10:23 -0500 5, 32 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s71CAL3Z031923 5, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Aug 2014 08:10:21 -0400 5, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 5, 32 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.181.6; Fri, 1 Aug 2014 08:10:21 -0400 5, 32 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 5, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.181.6; Fri, 1 Aug 2014 08:10:20 -0400 5, 32 -- Message-ID: {53DB83AD.1000109-at-cmich.edu} 5, 32 -- Date: Fri, 1 Aug 2014 08:10:21 -0400 5, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: micro {Microscopy-at-microscopy.com} 5, 32 -- Subject: Re: [Microscopy] Coping with problem relocation... 5, 32 -- References: {201408010646.s716kje9010947-at-ns.microscopy.com} 5, 32 -- In-Reply-To: {201408010646.s716kje9010947-at-ns.microscopy.com} 5, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit 5, 32 -- X-Originating-IP: [141.209.160.122] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -0.40 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 02MxoalqR - c9b43edbf820 - 20140801 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Dear Anza, most former dealing companies (like SIGMA-Aldrich or also [http://www.labome.com/product/US-Biological/C8210.html] =} "Whoops! Sorry about that. This product no longer appears to be in our catalog.") ) stopped cancelled this product in their catalogues,
[Labome: http://www.labome.com/bin/ea.pl?link=pd1619103 US Biological P.O. Box 261 Swampscott, Massachusetts 01907 service-at-usbio.net www.usbio.net 800-520-3011 headquarters: USA
Order No.: C8210 product name: Cupromeronic Blue xAA Size: 100 mg List Price: 125 USD ]
but I found CUPROLINIC BLUE (and know this dye has been used also for PG-staining in T-EM), which perhaps may serve the matter too: http://www.buyersguidechem.com/Asimil.php?suchname=cupromeronic%20blue http://www.buyersguidechem.com/AliefAus.php?pnumm=231952483398 :
Suppliers for Cuprolinic blue: 1 listed:
{ {Chemos GmbH Germany } } go to: http://www.buyersguidechem.com/AfirmAus.php?fnumm=04279127&pnumm=231952483398 Address Chemos GmbH Werner-von-Siemens-Str. 3 D-93128 Regenstauf, Germany Phone +49-9402-9336 0 Fax +49-9402-933 613 send an inquiry Homepage www.chemos-group.com
Another possibility perhaps: http://www.alfa-chemistry.com/cas_81207-55-8.htm Phone: 1-201-478-8534 Fax: 1-516-927-0118 Email: info-at-alfa-chemistry.com For product inquiries, please use our online system or send an email toinquiry-at-alfa-chemistry.com For order information, please contactsales-at-alfa-chemistry.com For other customer services, please contact customer-at-alfa-chemistry.com
Further information perhaps via GOOGELING: {Cupromeronic blue} or {Cuprolinic blue}
Best regards and wishes, Wolfgang
Wolfgang MUSS SALZBURG, AUSTRIA
Von: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] Gesendet: Freitag, 01. August 2014 13:56 An: Muß Wolfgang Betreff: [Microscopy] Cupromeronic Blue [staining in EM for PG's, any source?]
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We would like to stain proteoglycans in tissue with cupromeronic blue for EM studies. Does anyone know which companies are still selling cupromeronic blue?
There are many other stains used for staining proteoglycans such as Alican Blue, Toluidine Blue, Ruthenium Red, safranin O etc? Which one works best? Any recommendation would be appreciated.
Thank you
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Rosemary; Putting microscopes near an elevator would be a disaster. The room may pass EMI specs, but the large ferromagnetic mass alters the earth's magnetic field for quite a distance, so when the cars go up and down, the alignment and focus of the microscope will change and the image may move.
John Mardinly, ASU
-----Original Message----- X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Sent: Thursday, July 31, 2014 11:44 PM To: John Mardinly
Dear all,
Any hints as to how to handle relocation into a new building, in a smallish area between the four main lift (elevator) shafts, and next to the heaviest traffic corridor from the main loading dock. Building will house 600+ scientists, etc. which gives an idea of the traffic. The architects, and presumably our corporate overlords, appear to have locked into the building design, and say the microscopes just have to go in this place.
My preferred option is to insist on external specialist consultants to design something that will elminate or at least minimise any vibration and electromagnetic interference. Any other suggestions?
thanks, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
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==============================Original Headers============================== 17, 38 -- From John.Mardinly-at-asu.edu Fri Aug 1 16:27:28 2014 17, 38 -- Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) 17, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s71LRRss018814 17, 38 -- for {Microscopy-at-Microscopy.com} ; Fri, 1 Aug 2014 16:27:27 -0500 17, 38 -- X-ASG-Debug-ID: 1406928445-0549c43b79131d30001-FOsErg 17, 38 -- Received: from exhubt01.asurite.ad.asu.edu (exhubt01.asurite.ad.asu.edu [129.219.103.40]) by bcnetw3.asu.edu with ESMTP id IAJ9A742asMbwiqQ (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Fri, 01 Aug 2014 14:27:25 -0700 (MST) 17, 38 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 17, 38 -- X-Barracuda-Apparent-Source-IP: 129.219.103.40 17, 38 -- X-ASG-Whitelist: Client 17, 38 -- Received: from EXMBT03.asurite.ad.asu.edu ([169.254.3.52]) by 17, 38 -- exhubt01.asurite.ad.asu.edu ([129.219.103.40]) with mapi id 14.03.0174.001; 17, 38 -- Fri, 1 Aug 2014 14:27:25 -0700 17, 38 -- From: John Mardinly {John.Mardinly-at-asu.edu} 17, 38 -- To: "Rosemary.White-at-csiro.au" {Rosemary.White-at-csiro.au} , 17, 38 -- "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 17, 38 -- Subject: RE: [Microscopy] Coping with problem relocation... 17, 38 -- Thread-Topic: [Microscopy] Coping with problem relocation... 17, 38 -- X-ASG-Orig-Subj: RE: [Microscopy] Coping with problem relocation... 17, 38 -- Thread-Index: AQHPrVQGXLeEe2AwLku0Dr7P38OYIZu8QtGQ 17, 38 -- Date: Fri, 1 Aug 2014 21:27:24 +0000 17, 38 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE94015F7ECB2-at-exmbt03.asurite.ad.asu.edu} 17, 38 -- References: {201408010644.s716iJEU006836-at-ns.microscopy.com} 17, 38 -- In-Reply-To: {201408010644.s716iJEU006836-at-ns.microscopy.com} 17, 38 -- Accept-Language: en-US 17, 38 -- Content-Language: en-US 17, 38 -- X-MS-Has-Attach: 17, 38 -- X-MS-TNEF-Correlator: 17, 38 -- x-originating-ip: [129.219.103.4] 17, 38 -- Content-Type: text/plain; charset="us-ascii" 17, 38 -- MIME-Version: 1.0 17, 38 -- X-Barracuda-Connect: exhubt01.asurite.ad.asu.edu[129.219.103.40] 17, 38 -- X-Barracuda-Start-Time: 1406928445 17, 38 -- X-Barracuda-Encrypted: AES128-SHA 17, 38 -- X-Barracuda-URL: http://149.169.2.73:8000/cgi-mod/mark.cgi 17, 38 -- X-Virus-Scanned: by bsmtpd at asu.edu 17, 38 -- X-Barracuda-BRTS-Status: 1 17, 38 -- Content-Transfer-Encoding: 8bit 17, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s71LRRss018814 ==============================End of - Headers==============================
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Damage of organelles in whole mounts for TEM
Message: Hello All, We have isolated organelles and mounted them onto Formvar-carbon grids to prepare them for surface immunogold staining. Upon observation, we note that the organelles are quite damaged. We used a 4% PFA + 0.05% GTA fix in PBS, supplemented with 25mM HEPES to resuspend them and fixed them overnight. After immunostaining, we negatively stained them with 5% aq uranyl acetate. We do note some immunostaining, but our major concern is the quality and integrity of the organelle. Thanks, Vickie
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Email: nickmorrill-at-byu.edu Name: Nick Morrill
Organization: Brigham Young University/Precision Membranes
Title-Subject: [Filtered] Sample preparation using track-etched membranes
Message: Hello,
I recently discovered that track-etched membranes are used in some cases to prepare samples before viewing in the SEM. We've developed a similar technology in our materials science research group at BYU and I'd like to better understand what types of applications currently use the track-etched membranes and how well they work for this purpose. We are trying to determine what commercial applications might exist for our technology and wondering if some of the differences between our membranes and the track-etched membranes are of any interest. If you have experience using them, could you please let me know the following:
Related to sample preparation for SEM imaging:
- For the preparation of which samples/sample types do you currently use track-etched membranes? What pore sizes/diameters are used?
- How well do the track-etched membranes work for what you are trying to accomplish? If there are any pain-points, what are they? What would make them better?
Thanks.
Nick Morrill nickmorrill-at-byu.edu
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Hi Vickie, just a comment on this: a few thoughts come into my mind. Isolation of organelles (which one?) from any kind of tissue (which one?) is always tricky and likely to be pretty harmful. They are taken out of their native environment (usually, they are protected inside a cell, inside a tissue), and during isolation, you disrupt the tisssue and the cells, inevitably - first damage - and then you centrifuge the organelles, repeatedly - second damage (most of the microorganisms we have looked at, suffer from centrifugation; some heavily, some a lot, some less). Most of the organelles (mito, chloropl.) are also damaged, and you fix the isolated, i.e. damaged organelles, don't you? second, 4%FA+0.05%GA is not the most rigorous fixative; I understand, for immuno, you want to use 'mild' fixation conditions, but accordingly, the organelles are not rigorously fixed; best would be cryofixation + cryoTEM, or 2% GA (not good for immuno) plus TEM at RT, although this involves air-drying!. -- Next, I understand that you use PBS, because this is the buffer in which your antibodies are in best condition (they are made in blood). But, your organelles do not like PBS: this is by no means a physiological buffer for a condition "inside the cytoplasm of the cell". Neither the pH, nor the ionic strength, nor the ionic composition does fit. To improve this, you would have to go into a deeper analysis (Literature?) of the composition of the cells of your tissue; tricky!! -- Finally, 5% Uac is neither necessary (far too high, IMO; 1 or 2% is sufficient), and by the way, why staining at all? the pH of UAc is not favorable (4.5) for organelles, and you will see the organelles without any staining, and the contrast between the gold and the organelles might even be better without UAc staining. Skip this. - and at the end, after application to the (plastic or carbon-coated) grid, you air-dry the organelles, after several steps which are not 'optimal' - again, this will result in 'less-than-optimal' structure preservation. A few steps can be improved - but do not expect too much. good luck, and kind regards! Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: http://www.imc2014.com/ 18th IMC 2014 in Prague, CZ http://www.mc2015.de DGE - Microscopy Conference 2015, Sept 6-11, Göttingen
==============================Original Headers============================== 3, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Sat Aug 2 03:06:13 2014 3, 25 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7286AsI009970 3, 25 -- for {Microscopy-at-microscopy.com} ; Sat, 2 Aug 2014 03:06:13 -0500 3, 25 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 3, 25 -- by localhost (Postfix) with SMTP id BC2F2451F9 3, 25 -- for {Microscopy-at-microscopy.com} ; Sat, 2 Aug 2014 10:06:09 +0200 (CEST) 3, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 3, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 3, 25 -- (Client CN "gwsmtp.uni-regensburg.de", Issuer "Uni Regensburg CA - G01" (not verified)) 3, 25 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTPS id 8834C44612 3, 25 -- for {Microscopy-at-microscopy.com} ; Sat, 2 Aug 2014 10:06:09 +0200 (CEST) 3, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 3, 25 -- with Novell_GroupWise; Sat, 02 Aug 2014 10:06:07 +0200 3, 25 -- Message-Id: {53DCB80D020000540004CADD-at-gwsmtp1.uni-regensburg.de} 3, 25 -- X-Mailer: Novell GroupWise Internet Agent 14.0.1 3, 25 -- Date: Sat, 02 Aug 2014 10:06:05 +0200 3, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 3, 25 -- To: {vakimler-at-med.wayne.edu} , 3, 25 -- "microscopy listserver" {Microscopy-at-microscopy.com} 3, 25 -- Subject: damage of organelles in whole mounts-TEM 3, 25 -- Mime-Version: 1.0 3, 25 -- Content-Type: text/plain; charset=UTF-8 3, 25 -- Content-Transfer-Encoding: 8bit 3, 25 -- Content-Disposition: inline ==============================End of - Headers==============================
From vemeoro-at-spdop.ru Sat Aug 2 13:40:28 2014 Return-Path: {vemeoro-at-spdop.ru} Received: from ppp95-165-54-194.pppoe.spdop.ru (ppp95-165-54-194.pppoe.spdop.ru [95.165.54.194]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s72IeQbM004667 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 2 Aug 2014 13:40:27 -0500 Message-ID: {1CF929CE.6310461-at-spdop.ru}
Thanks for all the useful feedback!!
I could also mention that the substation serving the building, plus the main switchroom and distributor room are all within 10 metres of the microscopes in the current building plan! The main electrical (and everything else) risers for this 4 (or 5, not sure, haven't seen final plans) storey building are 2 and 4 metres away. The elevators are closer at 2 and 3 metres away.
Fun and games!
thanks, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
From discounted_pharmacy3-at-internet-tools.com Sun Aug 3 03:48:11 2014 Return-Path: {discounted_pharmacy3-at-internet-tools.com} Received: from smtp4.internet-tools.com ([77.123.65.214]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s738m8h9030375 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 3 Aug 2014 03:48:09 -0500 Message-Id: {201408030848.s738m8h9030375-at-ns.microscopy.com}
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Immunogold and Grids
Message: Hello Experts, What is the BEST Formvar-carbon grid to use for immunogold and/or Fluoronanogold? It appears that nickel, nickel-asbestos and gold seem to be the winners. It is necessary to glow discharge the grids? Please write at your earliest convenience. I am labeling thin-sections (Lowicryl and LR White hard/medium) as well as isolated organelles. Best, Vickie
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In principle Nickel, Gold, Rhodium and even Copper grids (with caution) are all suitable, as long as the films on the grids are well made without holes and the film and grid are not damaged or bent during the incubation and transfer. For that reason: consider using a loop wide enough to hold a grid instead of using sharp-edged tweezers. Transfer of incubation solutions can be minimised by blotting.
Some considerations: Ni-grids are more solid than the other ones, which can be helpful when going through many steps in an incubation setup. Nickel has a disadvantage, in that it influences the electron beam and may require repeated adjustment of the astigmatism. They can sometimes magnetically stick to tweezers, which can be annoying. Gold grids are fine, especially for labelling that does not use silver enhancement, but they are soft and bend easily. If silver enhancement is used and if there is a hole in the film, you may get enhancement of your grid and debris over the sections. Many use regular copper grids. Even though copper may become oxidised over time and by chemicals in solutions, if your films are decent, those grids can be fine as well.
Good luck!
Jan
Jan Leunissen i: www.aurion.nl
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From kydihe-at-mtu-net.ru Tue Aug 5 02:46:09 2014 Return-Path: {kydihe-at-mtu-net.ru} Received: from ppp91-78-180-208.pppoe.mtu-net.ru (ppp91-78-180-208.pppoe.mtu-net.ru [91.78.180.208]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s757k7FQ006316 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 5 Aug 2014 02:46:08 -0500 Message-ID: {F1269CC4.3324744-at-mtu-net.ru}
X-from: kristian.molhave-at-nanotech.dtu.dk ()
X-from: joseph.heintz-at-covance.com ()
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Email: joseph.heintz-at-covance.com Name: Joe Heintz
Organization: Covance
Title-Subject: [Filtered] Leica UC-T Issue?
Message:
Hello All, Currently I am using a Leica UC-T and lately I have been trying to obtain thin sections from an epoxy block, but on the return stroke the sample face has been picking up water from the trough. After a section is made, typically with a bit of water on it, the section is lifted from the trough during the return stroke and while a new section is made the previous section is on top of the new section. I've tried a few things to remedy the issue; I have changed the knife angle a degree each way, cleaned the knife edge and backside, dried both the block and the back of the knife and then lowered the water level but to no avail. I also work with a Leica UC-6 (using the same diamond knife, in the same room, on the same day) and not had the difficulties I am having now with the UC-T. An observation, while working with the UC-6, and UC-7 at another facility, during the return stroke the block face can easily be seen since the arm retracts, what appears to be, a millimeter or two but on the UC-T the clearance of the specimen block and the knife blades appears to be nonexistent. Could this clearance be the cause of the block face to grab the water from the trough? Any way to increase the clearance length? Any suggestions would be appreciated. If any one has a UC-T manual already in PDF form and are willing to send it my way it would also be appreciated. Thanks for your time.
-Joe
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Aug 8 14:58:24 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s78JwLdY017744 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 8 Aug 2014 14:58:21 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 7119773E98F 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 8 Aug 2014 14:58:21 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id MiHsY8s3ubBn for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 8 Aug 2014 14:58:15 -0500 (CDT) 14, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 5A4DD73E982 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 8 Aug 2014 14:58:15 -0500 (CDT) 14, 28 -- Message-ID: {53E52BD7.4040606-at-microscopy.com} 14, 28 -- Date: Fri, 08 Aug 2014 14:58:15 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Leica UC-T Issue? 14, 28 -- References: {201408081634.s78GYqHK015209-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201408081634.s78GYqHK015209-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201408081634.s78GYqHK015209-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Besides carbon and oxygen what elements would you expect to find in a fingerprint, dander, or hair? When doing failure analysis I would like to know if there are elements that I can look for and combine with other information that would point to poor handling or cleanliness vs some other cause.
Jonathan Abbott
==============================Original Headers============================== 15, 23 -- From jabbott-at-moxtek.com Fri Aug 8 15:44:59 2014 15, 23 -- Received: from hub031-co-7.exch031.serverdata.net (hub031-co-7.exch031.serverdata.net [199.193.204.141]) 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s78Kiv0J020137 15, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Aug 2014 15:44:58 -0500 15, 23 -- Received: from MBX031-W1-CO-8.exch031.domain.local ([10.224.113.89]) by 15, 23 -- HUB031-CO-7.exch031.domain.local ([10.224.113.58]) with mapi id 15, 23 -- 14.03.0174.001; Fri, 8 Aug 2014 13:43:55 -0700 15, 23 -- From: Jonathan Abbott {jabbott-at-moxtek.com} 15, 23 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 15, 23 -- Subject: EDS elements associated with people 15, 23 -- Thread-Topic: EDS elements associated with people 15, 23 -- Thread-Index: Ac+zSXglCQr4iaADRkqFniSllsrd1w== 15, 23 -- Date: Fri, 8 Aug 2014 20:43:54 +0000 15, 23 -- Message-ID: {23ACF4C758E77B4293F16E8F1E61F983445B199F-at-mbx031-w1-co-8.exch031.domain.local} 15, 23 -- Accept-Language: en-US 15, 23 -- Content-Language: en-US 15, 23 -- X-MS-Has-Attach: 15, 23 -- X-MS-TNEF-Correlator: 15, 23 -- x-originating-ip: [107.0.83.138] 15, 23 -- Content-Type: text/plain; charset="us-ascii" 15, 23 -- MIME-Version: 1.0 15, 23 -- Content-Transfer-Encoding: 8bit 15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s78Kiv0J020137 ==============================End of - Headers==============================
From eoeuoae-at-merope.org Fri Aug 8 16:03:01 2014 Return-Path: {eoeuoae-at-merope.org} Received: from smtp2.merope.org (localhost [113.181.44.146] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s78L2rYE021033 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 8 Aug 2014 16:02:58 -0500 Message-ID: {0310D40F.1101961-at-merope.org}
Besides C and O, Cl, Na, and K are common. N and P are not uncommon but generally at low levels.
Warren Straszheim
-----Original Message----- X-from: jabbott-at-moxtek.com [mailto:jabbott-at-moxtek.com] Sent: Friday, August 08, 2014 4:22 PM To: Straszheim, Warren E [BIOTC]
Besides carbon and oxygen what elements would you expect to find in a fingerprint, dander, or hair? When doing failure analysis I would like to know if there are elements that I can look for and combine with other information that would point to poor handling or cleanliness vs some other cause.
Jonathan Abbott
==============================Original Headers============================== 15, 23 -- From jabbott-at-moxtek.com Fri Aug 8 15:44:59 2014 15, 23 -- Received: from hub031-co-7.exch031.serverdata.net (hub031-co-7.exch031.serverdata.net [199.193.204.141]) 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s78Kiv0J020137 15, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Aug 2014 15:44:58 -0500 15, 23 -- Received: from MBX031-W1-CO-8.exch031.domain.local ([10.224.113.89]) by 15, 23 -- HUB031-CO-7.exch031.domain.local ([10.224.113.58]) with mapi id 15, 23 -- 14.03.0174.001; Fri, 8 Aug 2014 13:43:55 -0700 15, 23 -- From: Jonathan Abbott {jabbott-at-moxtek.com} 15, 23 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 15, 23 -- Subject: EDS elements associated with people 15, 23 -- Thread-Topic: EDS elements associated with people 15, 23 -- Thread-Index: Ac+zSXglCQr4iaADRkqFniSllsrd1w== 15, 23 -- Date: Fri, 8 Aug 2014 20:43:54 +0000 15, 23 -- Message-ID: {23ACF4C758E77B4293F16E8F1E61F983445B199F-at-mbx031-w1-co-8.exch031.domain.local} 15, 23 -- Accept-Language: en-US 15, 23 -- Content-Language: en-US 15, 23 -- X-MS-Has-Attach: 15, 23 -- X-MS-TNEF-Correlator: 15, 23 -- x-originating-ip: [107.0.83.138] 15, 23 -- Content-Type: text/plain; charset="us-ascii" 15, 23 -- MIME-Version: 1.0 15, 23 -- Content-Transfer-Encoding: 8bit 15, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s78Kiv0J020137 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 32 -- From wesaia-at-iastate.edu Sat Aug 9 10:33:22 2014 23, 32 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2lp0240.outbound.protection.outlook.com [207.46.163.240]) 23, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s79FXL1N015197 23, 32 -- for {Microscopy-at-microscopy.com} ; Sat, 9 Aug 2014 10:33:21 -0500 23, 32 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 23, 32 -- BLUPR04MB548.namprd04.prod.outlook.com (10.141.29.152) with Microsoft SMTP 23, 32 -- Server (TLS) id 15.0.1005.10; Sat, 9 Aug 2014 15:33:17 +0000 23, 32 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.36]) by 23, 32 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.36]) with mapi id 23, 32 -- 15.00.1005.008; Sat, 9 Aug 2014 15:33:17 +0000 23, 32 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 23, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 23, 32 -- Subject: RE: [Microscopy] EDS elements associated with people 23, 32 -- Thread-Topic: [Microscopy] EDS elements associated with people 23, 32 -- Thread-Index: AQHPs+FAHqa4FG3LJkqeOPseumQcu5vIZglA 23, 32 -- Date: Sat, 9 Aug 2014 15:33:17 +0000 23, 32 -- Message-ID: {9767f26efad5442393c2eb78dc96cc13-at-BLUPR04MB546.namprd04.prod.outlook.com} 23, 32 -- References: {201408082122.s78LMKX8022150-at-ns.microscopy.com} 23, 32 -- In-Reply-To: {201408082122.s78LMKX8022150-at-ns.microscopy.com} 23, 32 -- Accept-Language: en-US 23, 32 -- Content-Language: en-US 23, 32 -- X-MS-Has-Attach: 23, 32 -- X-MS-TNEF-Correlator: 23, 32 -- x-originating-ip: [97.64.202.142] 23, 32 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID:;UriScan:; 23, 32 -- x-forefront-prvs: 02981BE340 23, 32 -- x-forefront-antispam-report: SFV:NSPM;SFS:(6009001)(13464003)(377454003)(199002)(189002)(99286002)(106116001)(31966008)(19580395003)(19580405001)(83322001)(77982001)(2351001)(512954002)(33646002)(107046002)(79102001)(105586002)(107886001)(77096002)(106356001)(46102001)(81542001)(54356999)(110136001)(81342001)(50986999)(74662001)(74502001)(74316001)(89122001)(76176999)(21056001)(99396002)(76482001)(85852003)(15975445006)(83072002)(15202345003)(92566001)(4396001)(88552001)(75432001)(64706001)(2656002)(87936001)(86362001)(575784001)(101416001)(85306004)(95666004)(20776003)(66066001)(80022001)(76576001)(2501001)(24736002)(108616003);DIR:OUT;SFP:;SCL:1;SRVR:BLUPR04MB548;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;MX:1;LANG:en; 23, 32 -- Content-Type: text/plain; charset="us-ascii" 23, 32 -- MIME-Version: 1.0 23, 32 -- X-OriginatorOrg: iastate.edu 23, 32 -- Content-Transfer-Encoding: 8bit 23, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s79FXL1N015197 ==============================End of - Headers==============================
Other hobbyists and I are trying to gather up schematics, users manuals, etc for our SEMs (ex: my Super IIIA). I'm particularly interested in ISI SEMs. The ISI schematics we've gathered so far can be found here: http://siliconpr0n.org/wiki/doku.php?id=microscope:sem:isi:schem
A general material list can be found here: http://siliconpr0n.org/wiki/doku.php?id=microscope:sem:start
Please let me know if you have any material you can contribute to the repository.
John
==============================Original Headers============================== 4, 34 -- From johndmcmaster-at-gmail.com Sat Aug 9 11:34:33 2014 4, 34 -- Received: from mail-lb0-f178.google.com (mail-lb0-f178.google.com [209.85.217.178]) 4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s79GYVGR002675 4, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 9 Aug 2014 11:34:31 -0500 4, 34 -- Received: by mail-lb0-f178.google.com with SMTP id c11so4675851lbj.37 4, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 09 Aug 2014 09:34:30 -0700 (PDT) 4, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 34 -- d=gmail.com; s=20120113; 4, 34 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 34 -- :content-type:content-transfer-encoding; 4, 34 -- bh=186VUrLdziMl6NiAGzNCgk4UdfgKPtU/rxVOqj4IN24=; 4, 34 -- b=yUxwdlW+M2XwlHNeVzayFlEbhg1NQ3y0WvtS0s5UfACS7+OPqq2r2G3flsmiCGjtKB 4, 34 -- 6UousQOWi/GkLIID5PP253dnzjWWU7SU5t/2+4RoKTpPYN1bhfIY2hRA549207JX63O1 4, 34 -- CVRUCfCj5UzczQn+acpqwIwm9iKYp8ymvJkzYrN4du3tAPk8+bn6kndsfCFsk1Gb3qqh 4, 34 -- y8o0DAeLXHurgopDkJHbO8y44rWjQHV9YfJ0908+d1h/EtyNh08ko/InNqdHHaHCGYp3 4, 34 -- g8bUnWAVW2VlaT3YhpBFUEUdt5/GuN/ekeJu/U+gQ+rDHOrH6tQTXybkZlE3wrpnifWt 4, 34 -- jDSw== 4, 34 -- X-Received: by 10.152.43.65 with SMTP id u1mr27980641lal.5.1407602070261; 4, 34 -- Sat, 09 Aug 2014 09:34:30 -0700 (PDT) 4, 34 -- Received: from [46.246.37.63] (anon-37-63.vpn.ipredator.se. [46.246.37.63]) 4, 34 -- by mx.google.com with ESMTPSA id pz3sm9727740lbb.1.2014.08.09.09.34.27 4, 34 -- for {Microscopy-at-microscopy.com} 4, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 4, 34 -- Sat, 09 Aug 2014 09:34:28 -0700 (PDT) 4, 34 -- Message-ID: {53E64D92.10506-at-gmail.com} 4, 34 -- Date: Sat, 09 Aug 2014 09:34:26 -0700 4, 34 -- From: John McMaster {johndmcmaster-at-gmail.com} 4, 34 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 4, 34 -- MIME-Version: 1.0 4, 34 -- To: Microscopy-at-microscopy.com 4, 34 -- Subject: ISI SEM info 4, 34 -- X-Enigmail-Version: 1.6 4, 34 -- Content-Type: text/plain; charset=ISO-8859-1 4, 34 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From ziyogei-at-virginm.net Sun Aug 10 04:22:24 2014 Return-Path: {ziyogei-at-virginm.net} Received: from cpc7-blac7-2-0-cust223.pres.cable.virginm.net (cpc7-blac7-2-0-cust223.pres.cable.virginm.net [80.195.143.224]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7A9MN42012703 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 10 Aug 2014 04:22:24 -0500 Message-ID: {3EE81F2D.6414416-at-virginm.net}
Listers,
So, this isn't a microscopy question per se, but I always get what I need from you all, so I'm going to ask it here.
Does anyone know where (or even if) it is possible to buy substrates -- single and polycrystal -- of complex oxides? I'm thinking in terms of pyrochlores like Ti2Y2O7 and other middle-of-the-periodic table crystals Ti-Zr-ox, Zr-Hf-ox, etc. Carbides and nitrides (Cr23C6, TiC, TaC, TiN, TaN, etc.) would also be interesting.
Vendor responses welcome.
Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
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Message: On JEOL 1200EX, after entering 'asterisk mode' with CTRL+A, there are some commands that I am interested to know the function of:
- typing P or Q followed by a hexadecimal digit (0 to 9, A to F) displays a three byte value on the screen which differs for each input. This is also the case when one of 00, 60 or 80 is typed in. It appears to be modifiable by overtyping.
- typing one of CL, DA, EX, SC, ST or VA followed by ENTER causes an error but also emits a beep rather than the usual silence. I suspect that this indicates an incomplete command, as I know VAC to be the command for setting the vacuum system type. But I am curious to know if the others have any function.
Does anyone know what the purpose of these may be? They do not exist in the operating manual.
Sincerely, Tobias
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From ruoyze-at-mtu-net.ru Mon Aug 11 10:31:53 2014 Return-Path: {ruoyze-at-mtu-net.ru} Received: from ppp91-79-176-129.pppoe.mtu-net.ru (ppp91-79-176-129.pppoe.mtu-net.ru [91.79.176.129]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7BFVpKr023108 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 11 Aug 2014 10:31:52 -0500 Message-ID: {2B56D330.2254014-at-mtu-net.ru}
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Email: acruz04-at-cibnor.mx Name: Ariel Cruz
Organization: CIBNOR
Title-Subject: [Filtered] Capacitation SEM
"7° CURSO BÁSICO DE MICROSCOPÍA ELECTRÓNICA DE BARRIDO y avances en Microanálisis por sonda de rayos X" La Paz, Baja California Sur, México
We are invite you to take part in the basic course of electron microscopy to be conducted in the period from 25 to 29 August 2014, for more information check the page of CIBNOR,http://www.cibnor.mx/, in the events area, thank you and I thank you for your attention, greetings.
Ariel Cross Head of the laboratory of electron microscopy CIBNOR
Conducted in Spanish
Paulina Meza Navarro Departamento de Eventos CIBNOR, S.C. 52+ (612) 12 384-84 Ext. 3130
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Email: Tracy.Lawrence-at-inspection.gc.ca Name: Tracy Lawrence
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Title-Subject: [Filtered] Sticky specimen control knob
Message: The specimen control knob that moves the specimen in the y-plane has gradually become unresponsive and now will not move the specimen to the right at all. I have a Hitachi H-7100 TEM. Can anyone give me some ideas how to fix it? Thank you Tracy
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Email: djm Name: Dean J. Miller
Organization: ANL EMCenter - NanoScience and Technology Division
Title-Subject: [Filtered] Position Open - Facility Manager ANL EMCenter
Message: The Electron Microscopy Center at Argonne National Laboratory is seeking candidates for the position of Facility Manager. The Facility Manager will be responsible for:
- Managing day-to-day operations of the Electron Microscopy Center facilities for materials science research.
- Maintaining and ensuring effective performance of the capabilities operated within the EMC including TEM, SEM, FIB-SEM, ancillary equipment, and computers and software.
- Maintaining operational records for laboratory procedures, safety, user program and other requirements.
- Assisting in training users and supporting research as an analyst.
Considerable knowledge and experience with maintenance and operation of electron microscopes and related equipment, and materials science sample preparation for electron microscopy is expected. Familiarity with the operational and maintenance requirements of an experimental/scientific facility and specialized systems relative to materials science electron microscopy is desired.
Interested candidates should visit the following URL for further information and to submit an application for the position:
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On behalf of SCUR ( http://www.scur.org/content/e1163/e1164/index_ger.html ) I take the opportunity to inform you in time:
SAVE the DATE:
1st Announcement:
"42nd Annual Meeting of the Society for Cutaneous Ultrastructure Research, SCUR, MANNHEIM/Germany, 12th-14th of March 2015" ( http://www.scur.org/content/e1319//SCUR_Flyer_MANNHEIM_12_14th_March_2015.pdf )
General Topic: "Looking into and beyond the skin - wounds, stem cells and differentiation"
Location: Universitätsmedizin Mannheim Conference Centre "Alte Brauerei" Medical Faculty Mannheim, Heidelberg University Local Organizers: S.W. Schneider, I. Hausser-Siller, D. Breitkreutz, V. Huck
You will find an update, including Call for Abstracts, Registration and Accomodation forms, very soon in that place.
The SCUR (www.scur.org) looks forward to your participation and welcomes your questions / feedback / requests regarding the Meeting
sending an e-mail either to the Local Host Organisation, att. of Dr. Volker HUCK, E-Mail: volker.huck-at-medma.uni-heidelberg.de
or:
The Secretary of the SCUR Dr. Wolfgang MUSS (PhD) Member of MSA SALZBURG, AUSTRIA e-mail: w.muss-at-salk.at
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It is a long time since I operated a Hitachi 7000 series but your problem could be traced to what is a generic TEM problem?
The "O" ring on the specimen rod becomes dry over time, and, as this is the bearing on which the stage movement runs, the stage in that direction will become erratic. The control being used here is the right hand stage drive. If this is your problem, running a finger lightly coated in a good quality vacuum grease round the "O" ring will ease its movement. Once free the specimen rod will move into the microscope more quickly on specimen exchange, so take extra care!
If the problem is on the left hand stage drive take a close look at where the vertical shaft enters the pivot joint; does this move easily?
I hope this helps but if you need more please come back to me direct.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
X-from: Tracy.Lawrence-at-inspection.gc.ca ()
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Email: Tracy.Lawrence-at-inspection.gc.ca Name: Tracy Lawrence
Organization: Canadian Food Inspection Agency
Title-Subject: [Filtered] Sticky specimen control knob
Message: The specimen control knob that moves the specimen in the y-plane has gradually become unresponsive and now will not move the specimen to the right at all. I have a Hitachi H-7100 TEM. Can anyone give me some ideas how to fix it? Thank you Tracy
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==============================Original Headers============================== 23, 24 -- From protrain-at-emcourses.com Tue Aug 12 04:10:56 2014 23, 24 -- Received: from mail.esentra.net (mail.esentra.net [94.228.36.208]) 23, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7C9At5p015632 23, 24 -- for {microscopy-at-microscopy.com} ; Tue, 12 Aug 2014 04:10:55 -0500 23, 24 -- Received: from mail.clientmail.net (Not Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway 23, 24 -- id {B53e9da150001} ; Tue, 12 Aug 2014 10:10:45 +0100 23, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Tue, 12 Aug 2014 10:10:44 +0100 23, 24 -- Reply-To: {protrain-at-emcourses.com} 23, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} 23, 24 -- To: {Tracy.Lawrence-at-inspection.gc.ca} 23, 24 -- Cc: "'Microscopical Soc of America'" {microscopy-at-microscopy.com} 23, 24 -- References: {201408120301.s7C31fJl022181-at-ns.microscopy.com} 23, 24 -- In-Reply-To: {201408120301.s7C31fJl022181-at-ns.microscopy.com} 23, 24 -- Subject: RE: [Microscopy] viaWWW:Sticky specimen control knob 23, 24 -- Date: Tue, 12 Aug 2014 10:10:52 +0100 23, 24 -- Organization: Protrain 23, 24 -- Message-ID: {001601cfb60d$5186c2d0$f4944870$-at-com} 23, 24 -- MIME-Version: 1.0 23, 24 -- Content-Type: text/plain; 23, 24 -- charset="us-ascii" 23, 24 -- Content-Transfer-Encoding: 7bit 23, 24 -- X-Mailer: Microsoft Office Outlook 12.0 23, 24 -- Thread-Index: Ac+12bn+DLIFhAG8Rou5L/h2IHfTxwAMcdFQ 23, 24 -- Content-Language: en-gb ==============================End of - Headers==============================
It is a long time since I operated a Hitachi 7000 series but your problem could be traced to what is a generic TEM problem?
The "O" ring on the specimen rod becomes dry over time, and, as this is the bearing on which the stage movement runs, the stage in that direction will become erratic. The control being used here is the right hand stage drive. If this is your problem, running a finger lightly coated in a good quality vacuum grease round the "O" ring will ease its movement. Once free the specimen rod will move into the microscope more quickly on specimen exchange, so take extra care!
If the problem is on the left hand stage drive take a close look at where the vertical shaft enters the pivot joint; does this move easily?
I hope this helps but if you need more please come back to me direct.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
X-from: Tracy.Lawrence-at-inspection.gc.ca ()
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Email: Tracy.Lawrence-at-inspection.gc.ca Name: Tracy Lawrence
Organization: Canadian Food Inspection Agency
Title-Subject: [Filtered] Sticky specimen control knob
Message: The specimen control knob that moves the specimen in the y-plane has gradually become unresponsive and now will not move the specimen to the right at all. I have a Hitachi H-7100 TEM. Can anyone give me some ideas how to fix it? Thank you Tracy
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Aug 11 21:57:59 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7C2vxss020060 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 Aug 2014 21:57:59 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id F3391741150 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 Aug 2014 21:57:58 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id y9A+EHS3TqZ8 for {microscopy-at-microscopy.com} ; 12, 28 -- Mon, 11 Aug 2014 21:57:52 -0500 (CDT) 12, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id 2A8CE741143 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 Aug 2014 21:57:52 -0500 (CDT) 12, 28 -- Message-ID: {53E982B0.1070000-at-microscopy.com} 12, 28 -- Date: Mon, 11 Aug 2014 21:57:52 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Sticky specimen control knob 12, 28 -- References: {201408120028.s7C0ScHe018699-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201408120028.s7C0ScHe018699-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201408120028.s7C0ScHe018699-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 23, 24 -- From protrain-at-emcourses.com Tue Aug 12 08:12:32 2014 23, 24 -- Received: from mail.esentra.net (mail.esentra.net [94.228.36.208]) 23, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7C9At5p015632 23, 24 -- for {microscopy-at-microscopy.com} ; Tue, 12 Aug 2014 04:10:55 -0500 23, 24 -- Received: from mail.clientmail.net (Not Verified[172.16.2.209]) by mail.esentra.net with Esentra Mail Gateway 23, 24 -- id {B53e9da150001} ; Tue, 12 Aug 2014 10:10:45 +0100 23, 24 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Tue, 12 Aug 2014 10:10:44 +0100 23, 24 -- Reply-To: {protrain-at-emcourses.com} 23, 24 -- From: "Steve Chapman" {protrain-at-emcourses.com} 23, 24 -- To: {Tracy.Lawrence-at-inspection.gc.ca} 23, 24 -- Cc: "'Microscopical Soc of America'" {microscopy-at-microscopy.com} 23, 24 -- References: {201408120301.s7C31fJl022181-at-ns.microscopy.com} 23, 24 -- In-Reply-To: {201408120301.s7C31fJl022181-at-ns.microscopy.com} 23, 24 -- Subject: RE: [Microscopy] viaWWW:Sticky specimen control knob 23, 24 -- Date: Tue, 12 Aug 2014 10:10:52 +0100 23, 24 -- Organization: Protrain 23, 24 -- Message-ID: {001601cfb60d$5186c2d0$f4944870$-at-com} 23, 24 -- MIME-Version: 1.0 23, 24 -- Content-Type: text/plain; 23, 24 -- charset="us-ascii" 23, 24 -- Content-Transfer-Encoding: 7bit 23, 24 -- X-Mailer: Microsoft Office Outlook 12.0 23, 24 -- Thread-Index: Ac+12bn+DLIFhAG8Rou5L/h2IHfTxwAMcdFQ 23, 24 -- Content-Language: en-gb ==============================End of - Headers==============================
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SUBJECT: Artifacts in AFM Images, Livestream Seminar
The livestreaming AFM seminar series continues tomorrow with a discussion on Artifacts in AFM Images.
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==============================Original Headers============================== 10, 20 -- From pamela-at-afmworkshop.com Tue Aug 12 11:36:21 2014 10, 20 -- Received: from p3plwbeout23-05.prod.phx3.secureserver.net (p3plsmtp23-05-2.prod.phx3.secureserver.net [68.178.252.170]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7CGaKpi026250 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 12 Aug 2014 11:36:21 -0500 10, 20 -- Received: from localhost ([68.178.252.245]) 10, 20 -- by p3plwbeout23-05.prod.phx3.secureserver.net with bizsmtp 10, 20 -- id dscK1o0035JSNx801scKzp; Tue, 12 Aug 2014 09:36:19 -0700 10, 20 -- X-SID: dscK1o0035JSNx801 10, 20 -- Received: (qmail 2852 invoked by uid 99); 12 Aug 2014 16:36:19 -0000 10, 20 -- Content-Type: text/plain; charset="utf-8" 10, 20 -- X-Originating-IP: 66.214.45.45 10, 20 -- User-Agent: Workspace Webmail 5.7.2 10, 20 -- Message-Id: {20140812093618.636791cbb6cfeca4bdfcf4f7edb54753.bc42bf9d2b.wbe-at-email23.secureserver.net} 10, 20 -- From: "Pamela Stone" {pamela-at-afmworkshop.com} 10, 20 -- To: Microscopy-at-microscopy.com 10, 20 -- Subject: AFM: Tomorrow's Webinar on Artifacts in Images 10, 20 -- Date: Tue, 12 Aug 2014 09:36:18 -0700 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Transfer-Encoding: 8bit 10, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7CGaKpi026250 ==============================End of - Headers==============================
From qsyhuey-at-txun.net Tue Aug 12 17:43:41 2014 Return-Path: {qsyhuey-at-txun.net} Received: from mail4.txun.net (188.244.209.65.retail.sakhttk.ru [188.244.209.65] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7CMhcKU018590 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 12 Aug 2014 17:43:39 -0500 Message-ID: {5FC1EA1C.8133541-at-txun.net}
On Aug 9, 2014, at 12:32 AM, jabbott-at-moxtek.com wrote:
} Besides carbon and oxygen what elements would you expect to find in } a fingerprint, dander, or hair? When doing failure analysis I would } like to know if there are elements that I can look for and combine } with other information that would point to poor handling or } cleanliness vs some other cause.
Dear Jonathan, My high school chem teacher had an acronym for that: C HOPKINS CaFe. I'm not sure what the best of these--highest concentration in finger print and lowest in the environment--would be. I'd guess P or S. Yours, Bill
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Hair and (probably nails) will be unusually rich in sulfur compared to other tissues including skin. Hair proteins, keratins and keratin-associated proteins (or KAPs) contain a disproportionately high level of cysteine amino acid which has sulfur.
As I understand it (getting into less certain territory, but something you could follow up) hair also absorbs elevated levels of copper and possibly other heavy metals. Copper is the main one.
Other than that it would just be the same crew as for all biologically based material that Bill mentioned below.
Also, some of these samples, especially skin and finger prints, probably have high levels of lipids that (given enough finger prints) will be something you don't want inside a high vacuum system I would imagine.
Kind regards Duane
-----Original Message----- X-from: wtivol-at-sbcglobal.net [mailto:wtivol-at-sbcglobal.net] Sent: Wednesday, 13 August 2014 2:31 p.m. To: Harland, Duane
On Aug 9, 2014, at 12:32 AM, jabbott-at-moxtek.com wrote:
} Besides carbon and oxygen what elements would you expect to find in a } fingerprint, dander, or hair? When doing failure analysis I would like } to know if there are elements that I can look for and combine with } other information that would point to poor handling or cleanliness vs } some other cause.
Dear Jonathan, My high school chem teacher had an acronym for that: C HOPKINS CaFe. I'm not sure what the best of these--highest concentration in finger print and lowest in the environment--would be. I'd guess P or S. Yours, Bill
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both allan.mitchell-at-otago.ac.nz as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell
Organization: Microscopy Otago, Dunedin, New Zealand
Title-Subject: [Filtered] Electron microscopes on television and film
Message: Hi All
I am giving a talk to a general audience in two weeks and, as part of a light-hearted general introduction to electron microscopy, I was hoping to include a little about the appearance of electron microscopes on television and film.
There was some discussion on the listserver about this in early 2012 and I recall that it grew into quite a long list of movies and TV series.
I thought I had saved the list onto my computer but cannot now find it. I was wondering if anyone out there recalls saving the list and if, could send it to me.
What would be great also if anyone out there has any images (screen shots or similar) of scenes from movies or TV series with people at the electron microscope. If so, would it be possible to send me a copy of the image, or a link to it.
Many thanks
Have a great day.
Allan
PS, the talk is titled Continuity through Change, from an EM perspective. Not sure how I got myself into that one.
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254 EM Centre http://ocem.otago.ac.nz/
Microscopy Otago is hosting the 27th New Zealand Conference on Microscopy, 2 - 4 February 2015.
Please visit http://microscopy2015.otago.ac.nz/ for more details.
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==============================Original Headers============================== 28, 29 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 13 07:17:13 2014 28, 29 -- Received: from znl.com ([206.69.208.20]) 28, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7DCHDrZ001618 28, 29 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2014 07:17:13 -0500 28, 29 -- Received: from localhost (localhost [127.0.0.1]) 28, 29 -- by znl.com (Postfix) with ESMTP id 17B4A7420A7 28, 29 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2014 07:17:13 -0500 (CDT) 28, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 28, 29 -- Received: from znl.com ([127.0.0.1]) 28, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 28, 29 -- with ESMTP id SLqXdRU7KchS for {microscopy-at-microscopy.com} ; 28, 29 -- Wed, 13 Aug 2014 07:16:29 -0500 (CDT) 28, 29 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 28, 29 -- by znl.com (Postfix) with ESMTPA id 0FE8A742094 28, 29 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2014 07:16:29 -0500 (CDT) 28, 29 -- Message-ID: {53EB571D.7050207-at-microscopy.com} 28, 29 -- Date: Wed, 13 Aug 2014 07:16:29 -0500 28, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 28, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 28, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 28, 29 -- MIME-Version: 1.0 28, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} , 28, 29 -- MicroscopyListServer-Forward {microscopy-at-microscopy.com} 28, 29 -- Subject: viaWWW:Electron microscopes on television and film 28, 29 -- References: {201408130437.s7D4bjlk008810-at-ns.microscopy.com} 28, 29 -- In-Reply-To: {201408130437.s7D4bjlk008810-at-ns.microscopy.com} 28, 29 -- X-Forwarded-Message-Id: {201408130437.s7D4bjlk008810-at-ns.microscopy.com} 28, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 28, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Go to the Microscopy Listserver archives and do a simple search.
http://www.microscopy.com
Left hand Menu select SEARCH engine
Select YEAR = All of 2012
Select MESSAGE BODY enter "Movie"
Submit
Cheers,
Nestor
} Email: allan.mitchell-at-otago.ac.nz } } Name: Allan Mitchell } } Organization: Microscopy Otago, Dunedin, New Zealand } } Title-Subject: [Filtered] Electron microscopes on television and film } } Message: Hi All } } I am giving a talk to a general audience in two weeks and, as part of a light-hearted general introduction to electron microscopy, I was hoping to include a little about the appearance of electron microscopes on television and film. } } There was some discussion on the listserver about this in early 2012 and I recall that it grew into quite a long list of movies and TV series. } } I thought I had saved the list onto my computer but cannot now find it. I was wondering if anyone out there recalls saving the list and if, could send it to me. } } What would be great also if anyone out there has any images (screen shots or similar) of scenes from movies or TV series with people at the electron microscope. If so, would it be possible to send me a copy of the image, or a link to it. } } Many thanks } } Have a great day. } } Allan } } PS, the talk is titled Continuity through Change, from an EM perspective. Not sure how I got myself into that one. } } } } } Allan Mitchell } Microscopy Otago - Electron Microscopy } c/- Department of Anatomy } Otago School of Medical Sciences } P.O. Box 913 } Dunedin } New Zealand } } Phone (03) 479 5642 or 479 7301 } Fax (03) 479 5086 or 479 7254 } EM Centre } http://ocem.otago.ac.nz/
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
Titles of MSA-Listserver-Messages (1,2,3 = number of all messages -including original question- in my files) } TEM in "Avatar" ? { (5) (started Wedn 27.01.2010 20:53) } EMs in the Movies { (20) (started Mo,23.01.2012 23:01) } Updated Movie List { (1) (= [Microscopy] EMs in the Movies ) (Di 24.01.2012 00:01), which I copied and pasted here for your convenience:
====================================================== "So far thanks to over 60 responses (and a quick trawl of the archives), this is great keep them coming! We are up to the following:
1. Flash Gordon 2. Independence Day 3. The Man in White Suit 4. Predator 2 5. CSI 6. Spiderman 2 7. Blade Runner 8. NCIS 9. Dexter 10. Contagion 11. Andromeda Strain The Andromeda Strain (1971)and then the remake 2008 tv-miniseries. 12. Quincy 13. X-Files 14. Pres Obama at Intel's Titan 15. Spiderman 1 16. American Pickers 17. The Last Mimzy 18 ALTO MEDIA 'travel' into the matter. 19. special effects for one of the Star Trek 20. Batman 21. Jurassic Park 22. The Bone Collector" analyzes something 23. Avatar 24. The Prisoner (British TV series) 25. The Naked Gun
Thanks for all the responses so far! Once collected I will put together a website and send the link to the list. John (from john.mitchels-at-gmail.com ) I see a kind of duplicity in names here (:-)) ======================================== NB: this list has been completed with other titles in the message thread: " EMs in the Movies"(see above)
So you might try to contact {John Mitchels} , perhaps he has in file (over 60 responses never were displayed or sent via the MSA-Listserver).
If you would like and give permission to send to you the original Messages from MSA-Listserver as .msg -forwarding to your email-Address, please just reply to me and advise or request. This is an action of 3 min. only.
very best regards and good luck....for the lecture/presentation, as well as because of your statement: "not sure how I got myself into that one"...
Wolfgang (retiring most likely in December 2014 with no successor in the EM-Lab, which presumably will be shut down afterwards)
Wolfgang MUSS Member of MSA SALZBURG, AUSTRIA
============================================================================================================== Von: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Gesendet: Mittwoch, 13. August 2014 14:26 An: Muß Wolfgang Betreff: [Microscopy] Electron microscopes on television and film and: Microscopy Otago is hosting the 27th New Zealand Conference on Microscopy, 2 - 4 February 2015.
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Hi All
I am giving a talk to a general audience in two weeks and, as part of a light-hearted general introduction to electron microscopy, I was hoping to include a little about the appearance of electron microscopes on television and film.
There was some discussion on the listserver about this in early 2012 and I recall that it grew into quite a long list of movies and TV series.
I thought I had saved the list onto my computer but cannot now find it. I was wondering if anyone out there recalls saving the list and if, could send it to me.
What would be great also if anyone out there has any images (screen shots or similar) of scenes from movies or TV series with people at the electron microscope. If so, would it be possible to send me a copy of the image, or a link to it.
Many thanks
Have a great day.
Allan
PS, the talk is titled Continuity through Change, from an EM perspective. Not sure how I got myself into that one.
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254 EM Centre http://ocem.otago.ac.nz/ Microscopy Otago is hosting the 27th New Zealand Conference on Microscopy, 2 - 4 February 2015. Please visit http://microscopy2015.otago.ac.nz/ for more details.
Login Host: 139.80.40.139 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
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==============================Original Headers============================== 28, 29 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 13 07:17:13 2014 28, 29 -- Received: from znl.com ([206.69.208.20]) 28, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7DCHDrZ001618 28, 29 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2014 07:17:13 -0500 28, 29 -- Received: from localhost (localhost [127.0.0.1]) 28, 29 -- by znl.com (Postfix) with ESMTP id 17B4A7420A7 28, 29 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2014 07:17:13 -0500 (CDT) 28, 29 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 28, 29 -- Received: from znl.com ([127.0.0.1]) 28, 29 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 28, 29 -- with ESMTP id SLqXdRU7KchS for {microscopy-at-microscopy.com} ; 28, 29 -- Wed, 13 Aug 2014 07:16:29 -0500 (CDT) 28, 29 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 28, 29 -- by znl.com (Postfix) with ESMTPA id 0FE8A742094 28, 29 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2014 07:16:29 -0500 (CDT) 28, 29 -- Message-ID: {53EB571D.7050207-at-microscopy.com} 28, 29 -- Date: Wed, 13 Aug 2014 07:16:29 -0500 28, 29 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 28, 29 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 28, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 28, 29 -- MIME-Version: 1.0 28, 29 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} , 28, 29 -- MicroscopyListServer-Forward {microscopy-at-microscopy.com} 28, 29 -- Subject: viaWWW:Electron microscopes on television and film 28, 29 -- References: {201408130437.s7D4bjlk008810-at-ns.microscopy.com} 28, 29 -- In-Reply-To: {201408130437.s7D4bjlk008810-at-ns.microscopy.com} 28, 29 -- X-Forwarded-Message-Id: {201408130437.s7D4bjlk008810-at-ns.microscopy.com} 28, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 28, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 38, 43 -- From W.Muss-at-salk.at Wed Aug 13 07:48:27 2014 38, 43 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 38, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7DCmQnw026710 38, 43 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2014 07:48:27 -0500 38, 43 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 38, 43 -- by hermes.salk.at (Postfix) with ESMTP id 9B6DF646B41 38, 43 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2014 14:48:25 +0200 (CEST) 38, 43 -- X-Scanned-By: SALK-Content-Filter 38, 43 -- Received: from hermes.salk.at ([127.0.0.1]) 38, 43 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 38, 43 -- with ESMTP id uBh5yaV0qAh0 for {microscopy-at-microscopy.com} ; 38, 43 -- Wed, 13 Aug 2014 14:48:25 +0200 (CEST) 38, 43 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 38, 43 -- by hermes.salk.at (Postfix) with ESMTP id 74831646B53 38, 43 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2014 14:48:25 +0200 (CEST) 38, 43 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 38, 43 -- by N1EX198.lks.local with ESMTP; 13 Aug 2014 14:58:14 +0200 38, 43 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 38, 43 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 38, 43 -- 14.02.0387.000; Wed, 13 Aug 2014 14:48:25 +0200 38, 43 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 38, 43 -- To: "microscopy-at-microscopy.com LISTSERVER MSA (microscopy-at-microscopy.com)" 38, 43 -- {microscopy-at-microscopy.com} 38, 43 -- Subject: [Microscopy] Re: Electron microscopes on television and film and: 38, 43 -- Microscopy Otago is hosting the 27th New Zealand Conference on Microscopy, 2 38, 43 -- - 4 February 2015. 38, 43 -- Thread-Topic: [Microscopy] Re: Electron microscopes on television and film 38, 43 -- and: Microscopy Otago is hosting the 27th New Zealand Conference on 38, 43 -- Microscopy, 2 - 4 February 2015. 38, 43 -- Thread-Index: AQHPtvTenEn+pDXWKEaM+eWxiG+4YA== 38, 43 -- Date: Wed, 13 Aug 2014 12:48:24 +0000 38, 43 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D54D293-at-N2DT131.lksdom21.lks.local} 38, 43 -- References: {201408131225.s7DCPrwV006070-at-ns.microscopy.com} 38, 43 -- In-Reply-To: {201408131225.s7DCPrwV006070-at-ns.microscopy.com} 38, 43 -- Accept-Language: de-DE, en-US 38, 43 -- Content-Language: de-DE 38, 43 -- X-MS-Has-Attach: 38, 43 -- X-MS-TNEF-Correlator: 38, 43 -- x-originating-ip: [192.168.42.2] 38, 43 -- Content-Type: text/plain; charset="iso-8859-1" 38, 43 -- MIME-Version: 1.0 38, 43 -- Content-Transfer-Encoding: 8bit 38, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7DCmQnw026710 ==============================End of - Headers==============================
Hi everyone, We are using a JEOL 2100F TEM. I have 3 questions! 1. What is "Angle" correction? (it is shown on "Alignment" options and grouped along "Tilt" and "shift", when "Angle is wrong my tilt wobbler will wobble in oval trajectory rather than on single line) 2. How badly can it affect resolution of STEM? (in other words, how important it is) 3. Shall I align it in Tilt or Shift mode? as when i align it in one of modes, it gets disoriented in other mode. Thank you Amit
==============================Original Headers============================== 1, 26 -- From amit.welcomes.u-at-gmail.com Thu Aug 14 02:11:44 2014 1, 26 -- Received: from mail-we0-f176.google.com (mail-we0-f176.google.com [74.125.82.176]) 1, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7E7BiKv011955 1, 26 -- for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2014 02:11:44 -0500 1, 26 -- Received: by mail-we0-f176.google.com with SMTP id q58so671667wes.35 1, 26 -- for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2014 00:11:43 -0700 (PDT) 1, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 26 -- d=gmail.com; s=20120113; 1, 26 -- h=mime-version:from:date:message-id:subject:to:content-type; 1, 26 -- bh=ga+YMWEvBGHWtRXd0AduEqGQh50VSEmY86gU58pZL80=; 1, 26 -- b=nIRRKwGJtW7LSV9pAhnMY5n9caJuzj08ji2fdcFXXUCv6XzCysr3Ge5N8Wk/M8BBpx 1, 26 -- BtHiZGsiNuRYx5TfanihxdjqzEpo7+990QIXz2osM2YLmKpSMThr6tKlEiPsGWAn8nzq 1, 26 -- 8pjSNPM1rIWTLPX3n8rqOvIX2meQuh4DQXPpfleS3x+9LfAPo+lUt7hwJM8s6kGsyLEI 1, 26 -- t/K52FaHLOSR8vFf+K9j/S/q0Xs8WPOo3SNwEMUMfpKRDa+ebsrrVusSOBtwPz2YIAhp 1, 26 -- 4YZ5R6+Qhl5PX16mlW6LnXmRchxxwrbfa8kwooC0/9ccjweO/FpYQr81Ywy2aGK0lojj 1, 26 -- NW/w== 1, 26 -- X-Received: by 10.194.63.37 with SMTP id d5mr8719022wjs.92.1408000303436; Thu, 1, 26 -- 14 Aug 2014 00:11:43 -0700 (PDT) 1, 26 -- MIME-Version: 1.0 1, 26 -- Received: by 10.194.78.204 with HTTP; Thu, 14 Aug 2014 00:11:23 -0700 (PDT) 1, 26 -- From: Amit Gupta {amit.welcomes.u-at-gmail.com} 1, 26 -- Date: Thu, 14 Aug 2014 12:41:23 +0530 1, 26 -- Message-ID: {CA+bx8_yKwKCzc=rfpUPq+ZzCF0=V95nKXwmCXeb--Hjehb1R+w-at-mail.gmail.com} 1, 26 -- Subject: "Angle" correction in Jeol TEM 1, 26 -- To: microscopy {microscopy-at-microscopy.com} 1, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
1) The tilt and shift alignments are making sure that you get pure tilt when you tilt and no beam shift and pure shift when you shift and no tilt. The reason that you have to do this is because they use the same sets of deflection coils to do both. For the 2100F, when correcting tilt in TEM image mode with the beam at crossover, you do X only with X-tilt wobbler and then Y only with Y-tilt wobbler. When you use the each deflector, it will move the two beams across the screen and you can bring them back to the center using the corresponding X or Y beam shift control. Of course, you stop when they are on top of each other. You do the Shift correction in diffraction mode with the condenser fully counter clockwise and the diffraction focused to a caustic spot and brought to the center of the screen with PLA control. Again, shift X wobbler with X only and shift Y wobbler with Y only. If you are doing the Tilt correction, X or Y, and the two spots are offset and go past each other, i.e. you can't get them to coincide, then you have to make the Angle correction. Adjust the two spots so that they are perpendicular to the direction of travel during the adjustment. (This should be the closest approach to each other.) Then switch to Angle with the X or Y wobbler still on and correct this offset, again, only using the same X or Y deflector knob. Once you get that set, it just doesn't change over months. The shift corrections on my instrument never seem to wonder very much at all and they line up. I don't know whether the Angle should be used in this mode or not. I've never had to do it.
2) Before aligning the STEM, you have to align the TEM. I assume that not having the TEM properly aligned would be bad for STEM. You are using beam deflection coils in STEM and you have to have the tilt and shift compensated.
3) As I said above, I've only seen it occur in Tilt correction and not the Shift correction procedure on my system. I will send you my alignment procedure in a separate email from work. If they are split when doing the shift correction, you might consider asking your JEOL service engineer about it. We've had the PM done several times since I've taken over the instrument and it is always perfect.
I hope this helps.
-Scott
----- Original Message -----
X-from: "amit welcomes u" {amit.welcomes.u-at-gmail.com} To: "Walck, Scott" {S.Walck-at-comcast.net} Sent: Thursday, August 14, 2014 3:36:45 AM
Hi everyone, We are using a JEOL 2100F TEM. I have 3 questions! 1. What is "Angle" correction? (it is shown on "Alignment" options and grouped along "Tilt" and "shift", when "Angle is wrong my tilt wobbler will wobble in oval trajectory rather than on single line) 2. How badly can it affect resolution of STEM? (in other words, how important it is) 3. Shall I align it in Tilt or Shift mode? as when i align it in one of modes, it gets disoriented in other mode. Thank you Amit  ==============================Original Headers============================== 1, 26 -- From amit.welcomes.u-at-gmail.com Thu Aug 14 02:11:44 2014 1, 26 -- Received: from mail-we0-f176.google.com (mail-we0-f176.google.com [74.125.82.176]) 1, 26 --         by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7E7BiKv011955 1, 26 --         for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2014 02:11:44 -0500 1, 26 -- Received: by mail-we0-f176.google.com with SMTP id q58so671667wes.35 1, 26 --     for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2014 00:11:43 -0700 (PDT) 1, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 26 --     d=gmail.com; s=20120113; 1, 26 --     h=mime-version:from:date:message-id:subject:to:content-type; 1, 26 --     bh=ga+YMWEvBGHWtRXd0AduEqGQh50VSEmY86gU58pZL80=; 1, 26 --     b=nIRRKwGJtW7LSV9pAhnMY5n9caJuzj08ji2fdcFXXUCv6XzCysr3Ge5N8Wk/M8BBpx 1, 26 --      BtHiZGsiNuRYx5TfanihxdjqzEpo7+990QIXz2osM2YLmKpSMThr6tKlEiPsGWAn8nzq 1, 26 --      8pjSNPM1rIWTLPX3n8rqOvIX2meQuh4DQXPpfleS3x+9LfAPo+lUt7hwJM8s6kGsyLEI 1, 26 --      t/K52FaHLOSR8vFf+K9j/S/q0Xs8WPOo3SNwEMUMfpKRDa+ebsrrVusSOBtwPz2YIAhp 1, 26 --      4YZ5R6+Qhl5PX16mlW6LnXmRchxxwrbfa8kwooC0/9ccjweO/FpYQr81Ywy2aGK0lojj 1, 26 --      NW/w== 1, 26 -- X-Received: by 10.194.63.37 with SMTP id d5mr8719022wjs.92.1408000303436; Thu, 1, 26 --  14 Aug 2014 00:11:43 -0700 (PDT) 1, 26 -- MIME-Version: 1.0 1, 26 -- Received: by 10.194.78.204 with HTTP; Thu, 14 Aug 2014 00:11:23 -0700 (PDT) 1, 26 -- From: Amit Gupta {amit.welcomes.u-at-gmail.com} 1, 26 -- Date: Thu, 14 Aug 2014 12:41:23 +0530 1, 26 -- Message-ID: {CA+bx8_yKwKCzc=rfpUPq+ZzCF0=V95nKXwmCXeb--Hjehb1R+w-at-mail.gmail.com} 1, 26 -- Subject: "Angle" correction in Jeol TEM 1, 26 -- To: microscopy {microscopy-at-microscopy.com} 1, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 37 -- From s.walck-at-comcast.net Thu Aug 14 18:57:23 2014 15, 37 -- Received: from qmta08.westchester.pa.mail.comcast.net (qmta08.westchester.pa.mail.comcast.net [76.96.62.80]) 15, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7ENvMas018288 15, 37 -- for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2014 18:57:22 -0500 15, 37 -- Received: from omta07.westchester.pa.mail.comcast.net ([76.96.62.59]) 15, 37 -- by qmta08.westchester.pa.mail.comcast.net with comcast 15, 37 -- id enLf1o0061GhbT858nxNY1; Thu, 14 Aug 2014 23:57:22 +0000 15, 37 -- Received: from resmail-ch2-280v.sys.comcast.net ([162.150.49.59]) 15, 37 -- by omta07.westchester.pa.mail.comcast.net with comcast 15, 37 -- id enxM1o0091GdifW3TnxMez; Thu, 14 Aug 2014 23:57:22 +0000 15, 37 -- Date: Thu, 14 Aug 2014 23:57:21 +0000 (UTC) 15, 37 -- From: "S.Walck" {s.walck-at-comcast.net} 15, 37 -- To: amit welcomes u {amit.welcomes.u-at-gmail.com} , 15, 37 -- MicroscopyListserver {microscopy-at-microscopy.com} 15, 37 -- Message-ID: {2051946646.600707.1408060641548.JavaMail.root-at-comcast.net} 15, 37 -- In-Reply-To: {201408140736.s7E7ajiw027805-at-ns.microscopy.com} 15, 37 -- References: {201408140736.s7E7ajiw027805-at-ns.microscopy.com} 15, 37 -- Subject: Re: [Microscopy] "Angle" correction in Jeol TEM 15, 37 -- MIME-Version: 1.0 15, 37 -- Content-Type: text/plain; charset=utf-8 15, 37 -- X-Originating-IP: [2601:a:80:eb:7081:c704:5d7:90c] 15, 37 -- X-Mailer: Zimbra 8.0.3_GA_5664 (ZimbraWebClient - IE7 (Win)/8.0.3_GA_5664) 15, 37 -- Thread-Topic: "Angle" correction in Jeol TEM 15, 37 -- Thread-Index: WOqyriC3HfH3bLkgGmNEvj/j7MQ3kA== 15, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 15, 37 -- s=q20140121; t=1408060642; 15, 37 -- bh=O+EGpk2EygM+HHkEHSJ0wwUeNmbqDeivsPTm3CNloZo=; 15, 37 -- h=Received:Received:Date:From:To:Message-ID:Subject:MIME-Version: 15, 37 -- Content-Type; 15, 37 -- b=asgdtFXuNWr5+P6RHxQfsBCDSjeqT8YoPZe6sJ/az5VdGE6AwaodykErmgLdg4XMJ 15, 37 -- ec1gkV2wL7GqHNI828Sn1pOalIhQmoVYBMckcCc1eqoFPEfJ6Qa4OFvx/6WHaunyDH 15, 37 -- l9aDCu6ekjac195AZbGTatJABB4sjw8vwaq8meg8Yh7feH3S5cz7vSWO0cKXxGkMEf 15, 37 -- Tvo0CcbaIrwI1pezjrShVbxv5E8EDfJ6mZqhqvGa+1pn0X8a5hlKhHqE8lUPp/xPAf 15, 37 -- rvTSwU0KX4AhZTZ5mGpPWU8W8DkfKtET8cpfjj6BI7rZ/2bKWEH8RFqXoDe//Ne1vc 15, 37 -- DOz9xiuX0kDrg== 15, 37 -- Content-Transfer-Encoding: 8bit 15, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7ENvMas018288 ==============================End of - Headers==============================
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On Aug 13, 2014, at 5:51 AM, microscopylistserver- noreply-at-microscopy.com wrote:
} } Email: allan.mitchell-at-otago.ac.nz } Name: Allan Mitchell } } Organization: Microscopy Otago, Dunedin, New Zealand } } Title-Subject: [Filtered] Electron microscopes on television and film } } Message: Hi All } } I am giving a talk to a general audience in two weeks and, as part } of a light-hearted general } introduction to electron microscopy, I was hoping to include a } little about the appearance of } electron microscopes on television and film. } } There was some discussion on the listserver about this in early 2012 } and I recall that it grew into } quite a long list of movies and TV series. } } I thought I had saved the list onto my computer but cannot now find } it. I was wondering if anyone } out there recalls saving the list and if, could send it to me. } } What would be great also if anyone out there has any images (screen } shots or similar) of scenes from } movies or TV series with people at the electron microscope. If so, } would it be possible to send me } a copy of the image, or a link to it. } } Many thanks } } Have a great day. } } Allan } } PS, the talk is titled Continuity through Change, from an EM } perspective. Not sure how I got myself } into that one. } } } } } Allan Mitchell } Microscopy Otago - Electron Microscopy } c/- Department of Anatomy } Otago School of Medical Sciences } P.O. Box 913 } Dunedin } New Zealand } } Phone (03) 479 5642 or 479 7301 } Fax (03) 479 5086 or 479 7254 } EM Centre http://ocem.otago.ac.nz/ } } Microscopy Otago is hosting the 27th New Zealand Conference on } Microscopy, 2 - 4 February 2015. } } Please visit http://microscopy2015.otago.ac.nz/ for more details. } } Dear Alan, Blade Runner has not only electron microscopy, but one of David Scharf's images, which is alleged to be a snake scale. Yours, Bill
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From psueycu-at-ertelecom.ru Sun Aug 17 03:13:15 2014 Return-Path: {psueycu-at-ertelecom.ru} Received: from dynamicip-40-78-95-43.pppoe.chelny.ertelecom.ru (dynamicip-40-78-95-43.pppoe.chelny.ertelecom.ru [95.78.40.43] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7H8DDBh009872 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 17 Aug 2014 03:13:14 -0500 Message-ID: {669CF51F.3077867-at-ertelecom.ru}
X-from: lyle.gordon-at-pnnl.gov ()
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Email: lyle.gordon-at-pnnl.gov Name: Lyle Gordon
Organization: Pacific Northwest National Lab
Title-Subject: [Filtered] Looking for an old/slightly broken TEM cryo holder
Message: Dear Microscopists,
I wanted to thank all the listserv members for their assistance with my previous inquiries. I've just started a post-doc working on in situ TEM at low temperatures I'm looking for an old or slightly broken LN2 cryo-cooling holder (any manufacturer) compatible. I would prefer a holder compatible with Philips/FEI TEMs but I might be able to work with other types. Maybe you have something in your lab from an old CM200 gathering dust or another holder that is broken or no longer used. I would greatly appreciate any donations of old junk and we might be able to purchase equipment if you have something you are looking to sell that is no longer used.
Please reply off list.
Thanks very much, Lyle
-- Lyle Gordon Pacific Northwest National Lab http://lylegordon.ca
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From bfylrynve-at-psqonline.org Tue Aug 19 14:40:08 2014 Return-Path: {bfylrynve-at-psqonline.org} Received: from smtp2.psqonline.org ([195.14.119.156]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7JJe5cU015802 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 19 Aug 2014 14:40:06 -0500 Message-ID: {3168E30B.9535009-at-psqonline.org}
X-from: swallace-at-ambientgroup.com ()
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Email: swallace-at-ambientgroup.com Name: Sean Wallace
Organization: Ambient Group, Inc
Title-Subject: [Filtered] Old Noran Detector on Hitachi 600AB -Hardware Issue
Message: Hello, we have an issue with our detector (Model 405B-3SST) and we've recently unmounted it from the TEM. Upon examination, the "nose" of the detector is wrapped in a material that looks like a thin foil. There is evidence of loss of this material also, as evidenced by flakes of "foil" sitting on the column as if the nose was perhaps making contact with internal parts and scraping off material. I have photos I can forward to anyone interested.
First question: What is this foil material and is it necessary? It looks like some may have flaked off and was the cause of beam blockage. I've been told that a silver epoxy has been used on some model of TEM detectors to adhere the Det “collimator” snout onto the Det tube end and this is my initial speculation
Second: would the lack of this foil material cause the detector to not operate?
I'm hoping the loss of our counts was due to simple physical blockage by the stray material. Of course the alignment issue now comes into play as these things should not be touching anything.
any comment would be appreciated.
Sincerely, SW
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 20 08:48:59 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7KDmxI8014312 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 08:48:59 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 3C961746BB8 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 08:48:59 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id ygTKZE5LsXgP for {microscopy-at-microscopy.com} ; 16, 28 -- Wed, 20 Aug 2014 08:48:54 -0500 (CDT) 16, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id E2989746BAF 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 08:48:54 -0500 (CDT) 16, 28 -- Message-ID: {53F4A746.6010000-at-microscopy.com} 16, 28 -- Date: Wed, 20 Aug 2014 08:48:54 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:Old Noran Detector on Hitachi 600AB -Hardware Issue 16, 28 -- References: {201408192158.s7JLwP0m025144-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201408192158.s7JLwP0m025144-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201408192158.s7JLwP0m025144-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 16, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: nicholas.ritchie-at-nist.gov Name: Nicholas Ritchie
Message: Recently I've had a spat of failures of various different silicon drift detectors and I'm wondering if my experience is typical. The detectors have been failing catastrophically with the symptom of high fast discriminator counts leading to 100% dead time (probably low energy noise). One day they work at spec, the next day they have failed with no obvious cause.
I'm wondering about other people's experiences? BOTH GOOD AND BAD. How long are your silicon drift detectors lasting? How do they fail?
Please respond to me directly at nicholas.ritchie-at-nist.gov as I don't want to slander any vendors. I'll collect the information and make it available in manner that doesn't identify vendors but will still provide value to the community.
EDS Vendor: (like Noran/Bruker/EDAX)
Module manufacturer: (like KETEK, Amptek, PN Detectors, Seiko, or unknown)
Lifetime date: Failed after ?? months / Still operating after ?? months.
Failure mode: (what were the symptoms? gradual/catastrophic?)
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==============================Original Headers============================== 20, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 20 20:39:15 2014 20, 28 -- Received: from znl.com ([206.69.208.20]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7L1dF3p009440 20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:39:15 -0500 20, 28 -- Received: from localhost (localhost [127.0.0.1]) 20, 28 -- by znl.com (Postfix) with ESMTP id 2E52C74707D 20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:39:15 -0500 (CDT) 20, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 20, 28 -- Received: from znl.com ([127.0.0.1]) 20, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 20, 28 -- with ESMTP id YVx5ljGPD7IQ for {microscopy-at-microscopy.com} ; 20, 28 -- Wed, 20 Aug 2014 20:39:10 -0500 (CDT) 20, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 20, 28 -- by znl.com (Postfix) with ESMTPA id 4BF1A747070 20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:39:10 -0500 (CDT) 20, 28 -- Message-ID: {53F54DBD.3040104-at-microscopy.com} 20, 28 -- Date: Wed, 20 Aug 2014 20:39:09 -0500 20, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 20, 28 -- MIME-Version: 1.0 20, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 28 -- Subject: viaWWW:SDD XEDS detectors going wonky 20, 28 -- References: {201408201432.s7KEWvBp001834-at-ns.microscopy.com} 20, 28 -- In-Reply-To: {201408201432.s7KEWvBp001834-at-ns.microscopy.com} 20, 28 -- X-Forwarded-Message-Id: {201408201432.s7KEWvBp001834-at-ns.microscopy.com} 20, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 20, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] SEM Hitachi SU80xx: flash current too low after power outage?
Message: Hi, i am hoping to get some help/info's from C-FEG user's (... best would be Hitachi S-4800 or SU80xx users): i have been recently on a vacation for 2,5 weeks; when returning to my workplace i was informed that there has been a power outage three days before my return. For this reason the instrument was completly powerless for this time period.
After resetting the power braker and doing the runup procedure according to the manual the IGP's were back to "perfect" vaccuum within a day (#1 close to end of display range, which means "0.0"x10^-08Pa; #3 only slightly worse.
I deceided to do the flashing manually and discovered the following: instead of "before vacation" flash current rising to ~35µA it has been going for the last three days only to 13µA; the timespan and the amplitude of the measured vacuum IGP1 during the flashing did not change: it goes up to ~6.5x10^-08Pa and decays within in a few seconds to less than 1x10^-08Pa
Imaging seems to be working "normal" ... but isn't this change strange?
best regards G.Glaßer
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 20 20:40:18 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7L1eIEM010161 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:40:18 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 7DCF774708D 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:40:18 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id zPh4K8W1c96d for {microscopy-at-microscopy.com} ; 16, 28 -- Wed, 20 Aug 2014 20:39:52 -0500 (CDT) 16, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id DF9DF747080 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:39:52 -0500 (CDT) 16, 28 -- Message-ID: {53F54DE8.1030608-at-microscopy.com} 16, 28 -- Date: Wed, 20 Aug 2014 20:39:52 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:SEM Hitachi SU80xx: flash current too low after power outage? 16, 28 -- References: {201408201443.s7KEhQiF001996-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201408201443.s7KEhQiF001996-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201408201443.s7KEhQiF001996-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: sarah-at-biobus.org Name: Sarah Weisberg
Organization: BioBus
Title-Subject: [Filtered] SEM prep for tardigrades (water bears)
Message: Hi,
I run a project called the BioBus, which is a mobile microscopy facility that we use primarily for teaching at K-12 schools. We have been lent the Hitachi 3030 tabletop SEM, and are trying to prepare tardigrades (water bears) for imaging. I have been having trouble finding a good protocol.
Has anyone ever prepared tardigrades for SEM imaging? If so, could you please send me a protocol?
Thanks, Sarah Weisberg BioBus Program Director
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 20 20:40:49 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7L1emdd010373 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:40:48 -0500 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id BEA2474709C 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:40:48 -0500 (CDT) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id EsUvk+XujF2o for {microscopy-at-microscopy.com} ; 19, 28 -- Wed, 20 Aug 2014 20:40:31 -0500 (CDT) 19, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id A434374708E 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:40:31 -0500 (CDT) 19, 28 -- Message-ID: {53F54E0F.9030604-at-microscopy.com} 19, 28 -- Date: Wed, 20 Aug 2014 20:40:31 -0500 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:SEM prep for tardigrades (water bears) 19, 28 -- References: {201408201506.s7KF65QJ002337-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201408201506.s7KF65QJ002337-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201408201506.s7KF65QJ002337-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: vitha-at-tamu.edu Name: Stan Vitha
Organization: Texas A&M University
Title-Subject: [Filtered] User manual for AO-925 microtome knife sharpener?
Message: Hallo, I just took possession of an old American Optical knife sharpener. the machine is complete, including the plates and abrasives, but is missing the user manual. if anybody has a manual for this or similar model, would you mind sending a PDF or paper copy my way?
Online search was only partially successful 9a half of a manual was found).
Thank you in advance!
Best regards,
Stan Vitha Microscopy and Imaging Center Texas A&M University
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==============================Original Headers============================== 20, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 20 20:41:23 2014 20, 28 -- Received: from znl.com ([206.69.208.20]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7L1fM59010598 20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:41:22 -0500 20, 28 -- Received: from localhost (localhost [127.0.0.1]) 20, 28 -- by znl.com (Postfix) with ESMTP id 9B6557470AA 20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:41:22 -0500 (CDT) 20, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 20, 28 -- Received: from znl.com ([127.0.0.1]) 20, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 20, 28 -- with ESMTP id W-rccQRkX62R for {microscopy-at-microscopy.com} ; 20, 28 -- Wed, 20 Aug 2014 20:41:17 -0500 (CDT) 20, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 20, 28 -- by znl.com (Postfix) with ESMTPA id A309D74709D 20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:41:17 -0500 (CDT) 20, 28 -- Message-ID: {53F54E3D.9050503-at-microscopy.com} 20, 28 -- Date: Wed, 20 Aug 2014 20:41:17 -0500 20, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 20, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 20, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 20, 28 -- MIME-Version: 1.0 20, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20, 28 -- Subject: viaWWW:User manual for AO-925 microtome knife sharpener? 20, 28 -- References: {201408202117.s7KLHNK4008332-at-ns.microscopy.com} 20, 28 -- In-Reply-To: {201408202117.s7KLHNK4008332-at-ns.microscopy.com} 20, 28 -- X-Forwarded-Message-Id: {201408202117.s7KLHNK4008332-at-ns.microscopy.com} 20, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 20, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: Tracy.Lawrence-at-inspection.gc.ca Name: Tracy Lawrence
Organization: Canadian Food Inspection Agency
Title-Subject: [Filtered] Wanted: Chiller for Hitachi H-7100 TEM
Message: Hello All I am looking for a used water-cooled chiller for our TEM. Must be 208V 60Hz and with a cooling capacity of 23,000 BTU/hr. Please contact me outside the listserv if you have one that might be suitable. Thank you
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==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 20 20:42:14 2014 11, 28 -- Received: from znl.com ([206.69.208.20]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7L1gCC2012833 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:42:12 -0500 11, 28 -- Received: from localhost (localhost [127.0.0.1]) 11, 28 -- by znl.com (Postfix) with ESMTP id 205C37470B9 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:42:12 -0500 (CDT) 11, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 28 -- Received: from znl.com ([127.0.0.1]) 11, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 28 -- with ESMTP id Z4IU5mHXhM+k for {microscopy-at-microscopy.com} ; 11, 28 -- Wed, 20 Aug 2014 20:42:07 -0500 (CDT) 11, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 11, 28 -- by znl.com (Postfix) with ESMTPA id 584247470AC 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:42:07 -0500 (CDT) 11, 28 -- Message-ID: {53F54E6F.7070104-at-microscopy.com} 11, 28 -- Date: Wed, 20 Aug 2014 20:42:07 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 11, 28 -- MIME-Version: 1.0 11, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:Wanted: Chiller for Hitachi H-7100 TEM 11, 28 -- References: {201408202203.s7KM3Qjx009140-at-ns.microscopy.com} 11, 28 -- In-Reply-To: {201408202203.s7KM3Qjx009140-at-ns.microscopy.com} 11, 28 -- X-Forwarded-Message-Id: {201408202203.s7KM3Qjx009140-at-ns.microscopy.com} 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From talureu-at-leemathews.com Thu Aug 21 00:38:46 2014 Return-Path: {talureu-at-leemathews.com} Received: from mail3.leemathews.com ([217.76.74.152]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7L5cgPs004326 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 21 Aug 2014 00:38:44 -0500 Message-ID: {26A6E7EE.1935996-at-leemathews.com}
} -----Ursprüngliche Nachricht----- } Von: Muß Wolfgang } Gesendet: Donnerstag, 21. August 2014 10:37 } An: microscopy-at-microscopy.com LISTSERVER MSA } (microscopy-at-microscopy.com) } Betreff: [Microscopy] Re: User manual for AO-925 microtome knife } sharpener? } } Dear Stan, } I only can subserve with an {AO 935 manual} ..this is approx. 8MB..if } you want to receive it [I don't want to fill your e-mail account } without your permission/prior fiat, please just mail to me. } } best, } Wolfgang } } Von: microscopylistserver-noreply-at-microscopy.com } [mailto:microscopylistserver-noreply-at-microscopy.com] } Gesendet: Donnerstag, 21. August 2014 03:53 } An: Muß Wolfgang } Betreff: [Microscopy] User manual for AO-925 microtome knife sharpener? } } ----------------------------------------------------------------------- } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } X-from: vitha-at-tamu.edu () } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both vitha-at-tamu.edu as well as the Microscopy } Listserver } ----------------------------------------------------------------------- } ---- } Email: vitha-at-tamu.edu } Name: Stan Vitha } Organization: Texas A&M University } Title-Subject: User manual for AO-925 microtome knife } sharpener? } Message: } } Hallo, } I just took possession of an old American Optical knife sharpener. the } machine is complete, } including the plates and abrasives, but is missing the user manual. if } anybody has a manual for } this or similar model, would you mind sending a PDF or paper copy my } way? } } Online search was only partially successful 9a half of a manual was } found). } } Thank you in advance! } } Best regards, } } Stan Vitha } Microscopy and Imaging Center } Texas A&M University } Login Host: 128.194.151.93 } Listserver Email Form V - 20120416 } ----------------------------------------------------------------------- } ---- } ---===[|]===--- } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } Microscopy-at-Microscopy.com } ============================================ } ==============================Original } Headers============================== } 20, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 20 } 20:41:23 2014 } 20, 28 -- Received: from znl.com ([206.69.208.20]) } 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s7L1fM59010598 } 20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:41:22 - } 0500 } 20, 28 -- Received: from localhost (localhost [127.0.0.1]) } 20, 28 -- by znl.com (Postfix) with ESMTP id 9B6557470AA } 20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:41:22 - } 0500 (CDT) } 20, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 20, 28 -- Received: from znl.com ([127.0.0.1]) } 20, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd- } new, port 10024) } 20, 28 -- with ESMTP id W-rccQRkX62R for {microscopy-at-microscopy.com} ; } 20, 28 -- Wed, 20 Aug 2014 20:41:17 -0500 (CDT) } 20, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 20, 28 -- by znl.com (Postfix) with ESMTPA id A309D74709D } 20, 28 -- for {microscopy-at-microscopy.com} ; Wed, 20 Aug 2014 20:41:17 - } 0500 (CDT) } 20, 28 -- Message-ID: {53F54E3D.9050503-at-microscopy.com} } 20, 28 -- Date: Wed, 20 Aug 2014 20:41:17 -0500 } 20, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver- } noreply-at-microscopy.com} } 20, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 20, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; } rv:24.0) Gecko/20100101 Thunderbird/24.6.0 } 20, 28 -- MIME-Version: 1.0 } 20, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 20, 28 -- Subject: viaWWW:User manual for AO-925 microtome knife } sharpener? } 20, 28 -- References: {201408202117.s7KLHNK4008332-at-ns.microscopy.com} } 20, 28 -- In-Reply-To: {201408202117.s7KLHNK4008332-at-ns.microscopy.com} } 20, 28 -- X-Forwarded-Message-Id: } {201408202117.s7KLHNK4008332-at-ns.microscopy.com} } 20, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 20, 28 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 38 -- From W.Muss-at-salk.at Thu Aug 21 03:39:01 2014 4, 38 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 4, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7L8d1mr007336 4, 38 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2014 03:39:01 -0500 4, 38 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 4, 38 -- by hermes.salk.at (Postfix) with ESMTP id 9B320646B5B 4, 38 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2014 10:39:00 +0200 (CEST) 4, 38 -- X-Scanned-By: SALK-Content-Filter 4, 38 -- Received: from hermes.salk.at ([127.0.0.1]) 4, 38 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 4, 38 -- with ESMTP id OfwD_T7CGfI7 for {microscopy-at-microscopy.com} ; 4, 38 -- Thu, 21 Aug 2014 10:39:00 +0200 (CEST) 4, 38 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 4, 38 -- by hermes.salk.at (Postfix) with ESMTP id 7625C646B6D 4, 38 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2014 10:39:00 +0200 (CEST) 4, 38 -- Received: from n1dt134.lksdom21.lks.local ([192.168.121.134]) 4, 38 -- by N1EX198.lks.local with ESMTP; 21 Aug 2014 10:48:50 +0200 4, 38 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 4, 38 -- N1DT134.lksdom21.lks.local ([fe80::e8c4:8c7e:530e:3b7e%18]) with mapi id 4, 38 -- 14.02.0387.000; Thu, 21 Aug 2014 10:39:00 +0200 4, 38 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 4, 38 -- To: "'microscopy-at-microscopy.com LISTSERVER MSA (microscopy-at-microscopy.com)'" 4, 38 -- {microscopy-at-microscopy.com} 4, 38 -- Subject: [Microscopy] Re: User manual for AO-925 microtome knife sharpener? 4, 38 -- Thread-Topic: [Microscopy] Re: User manual for AO-925 microtome knife 4, 38 -- sharpener? 4, 38 -- Thread-Index: AQHPvRshSNtM/C6Mj06flE1i+TUcZZvavGyA 4, 38 -- Date: Thu, 21 Aug 2014 08:38:59 +0000 4, 38 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D58EC78-at-N2DT131.lksdom21.lks.local} 4, 38 -- Accept-Language: de-DE, en-US 4, 38 -- Content-Language: de-DE 4, 38 -- X-MS-Has-Attach: 4, 38 -- X-MS-TNEF-Correlator: 4, 38 -- x-originating-ip: [192.168.42.2] 4, 38 -- Content-Type: text/plain; charset="iso-8859-1" 4, 38 -- MIME-Version: 1.0 4, 38 -- Content-Transfer-Encoding: 8bit 4, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7L8d1mr007336 ==============================End of - Headers==============================
From meds-popular15-at-perfumania.com Thu Aug 21 05:07:08 2014 Return-Path: {meds-popular15-at-perfumania.com} Received: from smtp4.perfumania.com (pool.luga.net.ua [178.214.179.86] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7LA72bE027715 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 21 Aug 2014 05:07:04 -0500 Message-Id: {201408211007.s7LA72bE027715-at-ns.microscopy.com}
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Message: Can some one give me some perspective on the value (pros and cons) of SPIP in comparison to other SPM software available? The others I am familiar with are GWYDDION, WSXM, and Nanoscope (Bruker analysis package). It would also be nice to get the groups perspective on these software packages in comparison to Image J. Please note: I have seen the information on AFMHELP.COM. IÂ’m looking for a more in-depth perspective as we are considering purchasing SPIP.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Aug 21 07:59:47 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7LCxlcr030918 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2014 07:59:47 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id 4304574763C 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2014 07:59:47 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id M2dpaU6EGkES for {microscopy-at-microscopy.com} ; 12, 28 -- Thu, 21 Aug 2014 07:59:37 -0500 (CDT) 12, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id C6430747628 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2014 07:59:37 -0500 (CDT) 12, 28 -- Message-ID: {53F5ED39.9020008-at-microscopy.com} 12, 28 -- Date: Thu, 21 Aug 2014 07:59:37 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:SPM Image Analysis Software 12, 28 -- References: {201408210851.s7L8pwZ6014412-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201408210851.s7L8pwZ6014412-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201408210851.s7L8pwZ6014412-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 12, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hooke College of Applied Sciences, located in Westmont, IL, is offering a Polarizing Light Microscopy short course September 15-19, 2014. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.
For further PLM training details and registration information, please follow the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax)
==============================Original Headers============================== 7, 42 -- From CGorman-at-hookecollege.com Thu Aug 21 11:21:56 2014 7, 42 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 7, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7LGLuVe020827 7, 42 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2014 11:21:56 -0500 7, 42 -- X-ASG-Debug-ID: 1408638115-07bbd4604848550001-4CH8be 7, 42 -- Received: from TMGEX2.tmg.mccrone.com ([192.168.101.54]) by spam.mccrone.com with ESMTP id wTUbNq8jyGp50I23 for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2014 11:21:55 -0500 (CDT) 7, 42 -- X-Barracuda-Envelope-From: CGorman-at-hookecollege.com 7, 42 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by 7, 42 -- TMGEX2.tmg.mccrone.com (192.168.101.74) with Microsoft SMTP Server (TLS) id 7, 42 -- 15.0.913.22; Thu, 21 Aug 2014 11:21:54 -0500 7, 42 -- Received: from TMGEX1.tmg.mccrone.com ([fe80::cc0d:92d8:b3c7:8537]) by 7, 42 -- TMGEX1.tmg.mccrone.com ([fe80::d8ce:618:435a:28fe%16]) with mapi id 7, 42 -- 15.00.0913.011; Thu, 21 Aug 2014 11:21:42 -0500 7, 42 -- From: Christine Gorman {CGorman-at-hookecollege.com} 7, 42 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 7, 42 -- Subject: Polarized Light Microscopy Short Course 7, 42 -- Thread-Topic: Polarized Light Microscopy Short Course 7, 42 -- X-ASG-Orig-Subj: Polarized Light Microscopy Short Course 7, 42 -- Thread-Index: Ac+9W88WMJ92XqBOSgW7oEbYMbqDjg== 7, 42 -- Date: Thu, 21 Aug 2014 16:21:41 +0000 7, 42 -- Message-ID: {208b683a4c7049f6b798adc145aee00b-at-TMGEX1.tmg.mccrone.com} 7, 42 -- Accept-Language: en-US 7, 42 -- Content-Language: en-US 7, 42 -- X-MS-Has-Attach: 7, 42 -- X-MS-TNEF-Correlator: 7, 42 -- x-vipre-scanned: 1063856A007E9E106386B7 7, 42 -- x-originating-ip: [192.168.101.154] 7, 42 -- Content-Type: text/plain; charset="us-ascii" 7, 42 -- MIME-Version: 1.0 7, 42 -- X-Barracuda-Connect: UNKNOWN[192.168.101.54] 7, 42 -- X-Barracuda-Start-Time: 1408638115 7, 42 -- X-Barracuda-URL: http://spam.mccrone.com:80/cgi-mod/mark.cgi 7, 42 -- X-Virus-Scanned: by bsmtpd at mccrone.com 7, 42 -- X-Barracuda-BRTS-Status: 1 7, 42 -- X-Barracuda-Spam-Score: 0.00 7, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 7, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.8692 7, 42 -- Rule breakdown below 7, 42 -- pts rule name description 7, 42 -- ---- ---------------------- -------------------------------------------------- 7, 42 -- Content-Transfer-Encoding: 8bit 7, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7LGLuVe020827 ==============================End of - Headers==============================
Assistant Professor of Biochemistry University of Wisconsin-Madison: Imaging Molecules
Breakthroughs in the imaging of molecules at increased spatial or temporal resolution have linked classical biochemistry with studies at the cellular and organismal level. The Biochemistry Department at the University of Wisconsin-Madison (www.biochem.wisc.edu) seeks to hire an Assistant Professor (tenure track) in this area. The successful candidate will utilize state-of-art imaging methods to advance important areas in biochemistry, structural biology, cell biology or physiology. Approaches include but are not limited to: cryo-EM or correlative light-EM; electron tomography; super-resolution, single molecule or light sheet microscopy; atomic force microscopy; imaging with novel fluorescent or optical approaches. We seek individuals who employ these methods to address key issues concerning biological molecules, machines, and structures. The successful candidate will be expected to develop a vigorous, extramurally-funded research program, and to participate in teaching in the Department. Attractive recruitment packages include start-up funding, salary support, state-of-art core facilities, exceptional laboratory space, access to vibrant graduate training programs, and a collegial and collaborative environment.
Applicants should submit PDF files of curriculum vitae with summary of research accomplishments, description of future research program and teaching interests, and letters from three references to jobs-at-biochem.wisc.edu by October 15, 2014. Please note: Unless confidentiality is requested in writing, information regarding applicants must be released upon request. Finalists cannot be guaranteed confidentiality. UW-Madison is an equal opportunity/affirmative action employer. Department
-- Kevin W. Eliceiri Director, Laboratory for Optical and Computational Instrumentation (LOCI) Departments Cell and Molecular Biology and Biomedical Engineering Affiliate Principal Investigator, Morgridge Institute for Research (MIR) Room 271 Animal Sciences, 1675 Observatory Drive, Madison, WI 53706 Phone: 608-263-6288
==============================Original Headers============================== 7, 39 -- From eliceiri-at-wisc.edu Fri Aug 22 07:01:15 2014 7, 39 -- Received: from smtpauth2.wiscmail.wisc.edu (wmauth2.doit.wisc.edu [144.92.197.222]) 7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7MC1FZS020411 7, 39 -- for {Microscopy-at-microscopy.com} ; Fri, 22 Aug 2014 07:01:15 -0500 7, 39 -- MIME-version: 1.0 7, 39 -- Content-disposition: inline 7, 39 -- Content-type: text/plain; charset=iso-8859-1 7, 39 -- Received: from avs-daemon.smtpauth2.wiscmail.wisc.edu by 7, 39 -- smtpauth2.wiscmail.wisc.edu 7, 39 -- (Oracle Communications Messaging Server 7.0.5.30.0 64bit (built Oct 22 2013)) 7, 39 -- id {0NAP00500IERLH00-at-smtpauth2.wiscmail.wisc.edu} for 7, 39 -- Microscopy-at-microscopy.com; Fri, 22 Aug 2014 07:01:15 -0500 (CDT) 7, 39 -- X-Spam-PmxInfo: Server=avs-2, Version=6.0.3.2322014, 7, 39 -- Antispam-Engine: 2.7.2.2107409, Antispam-Data: 2014.8.22.114819, 7, 39 -- SenderIP=0.0.0.0 7, 39 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=0.0.0.0 7, 39 -- Received: from wiscmail.wisc.edu (unknown [144.92.8.221]) 7, 39 -- by smtpauth2.wiscmail.wisc.edu 7, 39 -- (Oracle Communications Messaging Server 7.0.5.30.0 64bit (built Oct 22 2013)) 7, 39 -- with ESMTPA id {0NAP001WDIQ2Z000-at-smtpauth2.wiscmail.wisc.edu} for 7, 39 -- Microscopy-at-microscopy.com; Fri, 22 Aug 2014 07:01:14 -0500 (CDT) 7, 39 -- Received: from [144.92.8.221] (Forwarded-For: 144.92.8.221) 7, 39 -- by wmweb1.doit.wisc.edu (mshttpd); Fri, 22 Aug 2014 07:01:14 -0500 7, 39 -- From: Kevin W Eliceiri {eliceiri-at-wisc.edu} 7, 39 -- To: Microscopy-at-microscopy.com 7, 39 -- Reply-to: eliceiri-at-wisc.edu 7, 39 -- Message-id: {74e096e7b3586.53f6eaba-at-wiscmail.wisc.edu} 7, 39 -- Date: Fri, 22 Aug 2014 07:01:14 -0500 7, 39 -- X-Mailer: Oracle Communications Messenger Express 7u4-25.01(7.0.4.25.0) 64bit 7, 39 -- (built Feb 29 2012) 7, 39 -- Content-language: en 7, 39 -- Subject: Position Announcement: Assistant Professor Imaging of Molecules: 7, 39 -- University of Wisconsin 7, 39 -- X-Accept-Language: en 7, 39 -- Priority: normal 7, 39 -- In-reply-to: {7790ad01b7740.53f730dc-at-wiscmail.wisc.edu} 7, 39 -- References: {7790ad01b7740.53f730dc-at-wiscmail.wisc.edu} 7, 39 -- Content-Transfer-Encoding: 8bit 7, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7MC1FZS020411 ==============================End of - Headers==============================
From nifqugva-at-sm-usa.org Fri Aug 22 07:05:50 2014 Return-Path: {nifqugva-at-sm-usa.org} Received: from smtp3.sm-usa.org ([37.29.40.190]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7MC5jJP025178 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 22 Aug 2014 07:05:47 -0500 Message-ID: {815818ED.9627102-at-sm-usa.org}
X-from: jhyun-at-gatan.com ()
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] EELS and EFTEM Training, October 2014
Message: EELS & EFTEM Analysis Training School. October 14-17, 2014. Gatan, Pleasanton, California, USA.
Want to refresh or advance your EELS and EFTEM knowledge? Join us for an intensive 4-day training school that incorporates lectures, computer laboratories, and microscope practicals to provide participants with comprehensive, hands-on training on key EELS and EFTEM topics and technology.
Online registration is now open at www.gatan.com/resources/training/EELSSchoolOct2014.php. School fee: $1,750.00 US Dollars. Space is limited.
Overview: Transmission electron microscopy (TEM) reveals details of natural and man-made structures at the micrometer, nanometer, and even sub-nanometer scale. Energy-filtered TEM (EFTEM) and electron energy-loss spectroscopy (EELS) are the ideal analytical partners to the high spatial resolution provided by TEM in both the conventional and scanned (STEM) imaging modes.
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Aug 23 07:36:33 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7NCaXeO023816 13, 28 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2014 07:36:33 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 2C6C3748BF1 13, 28 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2014 07:36:33 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id 4px+tZAwvalW for {microscopy-at-microscopy.com} ; 13, 28 -- Sat, 23 Aug 2014 07:36:24 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 58B59748BDF 13, 28 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2014 07:36:23 -0500 (CDT) 13, 28 -- Message-ID: {53F88AC7.8030102-at-microscopy.com} 13, 28 -- Date: Sat, 23 Aug 2014 07:36:23 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:EELS and EFTEM Training, October 2014 13, 28 -- References: {201408221908.s7MJ8dgR014466-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201408221908.s7MJ8dgR014466-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201408221908.s7MJ8dgR014466-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Chloroform Flattening of Sections
Message: When one chloroform-flattens ultrathin sections for the TEM, is there artifactual stretching and resultant alterations in the dimensions of structures, which are critical in morphometric work?
Does chloroform have a greater effect on LR White/Lowicryl versus the Embed and other harder non-immuno resins?
Finally, on a serendipitous note, would chloroform have the ability, if it does indeed stretch apart structures on a nano level, open up antigenic determinants for the often difficult immunogold work?
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From wikove-at-stv.ru Sun Aug 24 01:20:58 2014 Return-Path: {wikove-at-stv.ru} Received: from host-109-168-229-168.stv.ru (host-109-168-229-168.stv.ru [109.168.229.168] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7O6KuCa023704 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 24 Aug 2014 01:20:57 -0500 X-AntiVirus: Checked by Dr.Web [version: 7.0.4.09220, engine: 7.0.100.09170, virus records: 3630317, updated: 1.02.2013] Message-ID: {96C55A70.5094209-at-stv.ru}
I am sure overstretching is a risk but so is not stretching. To get a feel for the effects of compression on thin sections, look at the article by Studer and Gnägi (2000) Minimal compression of ultrathin sections with use of an oscillating diamond knife. Journal of Microscopy, Vol. 197(1):94-100. This article is about using a vibrating diamond knife to avoid compression but I remember it having good comparison images. Diatome's web page has an interesting discussion at http://www.diatomeknives.com/knives/pdf/ultrasonic_flyer_usa_1108.pdf. They say the compression of various resins are 10-20% for epoxy resins, 12-24% for Lowicryl K4M, 10-17% for Spurr's and8-13% for LR White. I don't use chloroform anymore. I am a big fan of the less toxic heat pens. I feel 120 V electrical-line models are much better than battery-operated ones.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Saturday, August 23, 2014 10:48 PM To: Phillips, Thomas E.
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Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Chloroform Flattening of Sections
Message: When one chloroform-flattens ultrathin sections for the TEM, is there artifactual stretching and resultant alterations in the dimensions of structures, which are critical in morphometric work?
Does chloroform have a greater effect on LR White/Lowicryl versus the Embed and other harder non-immuno resins?
Finally, on a serendipitous note, would chloroform have the ability, if it does indeed stretch apart structures on a nano level, open up antigenic determinants for the often difficult immunogold work?
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==============================Original Headers============================== 22, 29 -- From PhillipsT-at-missouri.edu Sun Aug 24 07:14:43 2014 22, 29 -- Received: from mst-rip6-missouri-out.um.umsystem.edu (mst-rip6-missouri-out.um.umsystem.edu [198.209.50.149]) 22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7OCEhKl029704 22, 29 -- for {microscopy-at-microscopy.com} ; Sun, 24 Aug 2014 07:14:43 -0500 22, 29 -- X-IronPort-Anti-Spam-Filtered: true 22, 29 -- X-IronPort-Anti-Spam-Result: AhQFAKvW+VPPoJ7I/2dsb2JhbABWA4MNIDNXBMw2CodNAYEIFneEAwEBAQICTjcCAgIBGQMBAQELAgoBEAcWGwEUCQgCBAoJCBUEiCENvTeESxcEjSYSgSgGEQEfIRILCwEBgiUPRCSBHQWGEYRVgm6DUoQphCCEMpM0gWWBeWwBAYEGBxcigQcBAQE 22, 29 -- X-IPAS-Result: AhQFAKvW+VPPoJ7I/2dsb2JhbABWA4MNIDNXBMw2CodNAYEIFneEAwEBAQICTjcCAgIBGQMBAQELAgoBEAcWGwEUCQgCBAoJCBUEiCENvTeESxcEjSYSgSgGEQEfIRILCwEBgiUPRCSBHQWGEYRVgm6DUoQphCCEMpM0gWWBeWwBAYEGBxcigQcBAQE 22, 29 -- Received: from um-tcas3.um.umsystem.edu ([207.160.158.200]) 22, 29 -- by mst-rip6-exch-relay.um.umsystem.edu with ESMTP; 24 Aug 2014 07:14:43 -0500 22, 29 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.77]) by 22, 29 -- UM-TCAS3.um.umsystem.edu ([207.160.158.200]) with mapi id 14.03.0181.006; 22, 29 -- Sun, 24 Aug 2014 07:14:42 -0500 22, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 22, 29 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 22, 29 -- Subject: [Microscopy] viaWWW:Chloroform Flattening of Sections 22, 29 -- Thread-Topic: [Microscopy] viaWWW:Chloroform Flattening of Sections 22, 29 -- Thread-Index: AQHPv04/ttSveQrdjE+HLflaxy62j5vfpymggAAB2cCAAAIPUA== 22, 29 -- Date: Sun, 24 Aug 2014 12:14:40 +0000 22, 29 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DDBEFE091-at-UM-MBX-T02.um.umsystem.edu} 22, 29 -- References: {201408240348.s7O3mIgj004413-at-ns.microscopy.com} 22, 29 -- Accept-Language: en-US 22, 29 -- Content-Language: en-US 22, 29 -- X-MS-Has-Attach: 22, 29 -- X-MS-TNEF-Correlator: 22, 29 -- x-originating-ip: [207.160.158.193] 22, 29 -- Content-Type: text/plain; charset="iso-8859-1" 22, 29 -- MIME-Version: 1.0 22, 29 -- Content-Transfer-Encoding: 8bit 22, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7OCEhKl029704 ==============================End of - Headers==============================
From cheapest-meds3-at-cfhnyc.org Sun Aug 24 11:21:27 2014 Return-Path: {cheapest-meds3-at-cfhnyc.org} Received: from smtp1.cfhnyc.org ([91.236.221.166]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7OGLODW023552 for {microscopylistserverarchive2-at-microscopy.com} ; Sun, 24 Aug 2014 11:21:25 -0500 Message-Id: {201408241621.s7OGLODW023552-at-ns.microscopy.com}
X-from: Kirsty.MacLellan-Gibson-at-nibsc.org ()
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Title-Subject: [Filtered] Position for confocal microscopist
Message: Dear Listers,
I have an opening for an experienced confocal microscopist, preferably with experience of at least one of the following techniques multiphoton microscopy, OPO lasers, 2nd and 3rd harmonic reduction imaging, live cell imaging FCS and or and FLIM. A functional knowledge of TEM is also desirable. Below is a summary of the role.
Purpose of Role: The post holder will be responsible for the confocal microscope and other light microscopes within the imaging group and for the training and development of the light microscopy side of the group. The post holder will report to the imaging group manager. Key Responsibilities: • Contribution of personal technical expertise and involvement in a least one of the key resource provisions of the section. • Data acquisition, processing and interpretation on collaborative research projects. • Sample preparation including: fluorescent, histochemical and immunological staining; live cell labelling; cell and or microbiological culture techniques and standard laboratory procedures. • Training of end users and the creation and update of SOP’s, COSHH and risk assessments’. • Developing and managing internal collaborations. • Maintenance of equipment and liaison with service engineers and company representatives. • Keeping up to date with advances in the field and actively participating in courses and conferences. • The option to pursue individual research interests.
Applications can be made via New Scientist, or the civil service jobs website (position 1420701) where you will be asked to fill in a massive form (please do not let that put you off applying).
The National Institute for Biological Standards and Control (NIBSC), a Centre of the Medicines and Healthcare Products Regulatory Agency (MHRA), is a world leader in assuring the quality of biological medicines through product testing, developing reference materials and applied research. Due to the nature of its role we bring together a wide range of scientists covering R&D, production and testing of reference materials and biological medicines and have numerous state of the art machines and techniques on site. NIBSC is located to the north of London and is surrounded by the beautiful Hertfordshire countryside, there are nearby transport links to London and Cambridge. Further information on NIBSC can be found at http://www.nibsc.org/ or by contacting myself.
All the best,
Kirsty
Kirsty MacLellan-Gibson
Head of Biological Imaging
National Institute for Biological Standards and Control (NIBSC) Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG UK Telephone: 01707 641503
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==============================Original Headers============================== 23, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Aug 26 07:18:09 2014 23, 28 -- Received: from znl.com ([206.69.208.20]) 23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7QCI9XB026748 23, 28 -- for {microscopy-at-microscopy.com} ; Tue, 26 Aug 2014 07:18:09 -0500 23, 28 -- Received: from localhost (localhost [127.0.0.1]) 23, 28 -- by znl.com (Postfix) with ESMTP id 10CA974B141 23, 28 -- for {microscopy-at-microscopy.com} ; Tue, 26 Aug 2014 07:18:09 -0500 (CDT) 23, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 23, 28 -- Received: from znl.com ([127.0.0.1]) 23, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 23, 28 -- with ESMTP id IiakZXmwk1BX for {microscopy-at-microscopy.com} ; 23, 28 -- Tue, 26 Aug 2014 07:18:03 -0500 (CDT) 23, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 23, 28 -- by znl.com (Postfix) with ESMTPA id B159274B12F 23, 28 -- for {microscopy-at-microscopy.com} ; Tue, 26 Aug 2014 07:18:03 -0500 (CDT) 23, 28 -- Message-ID: {53FC7AFB.3030906-at-microscopy.com} 23, 28 -- Date: Tue, 26 Aug 2014 07:18:03 -0500 23, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 23, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 23, 28 -- MIME-Version: 1.0 23, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 23, 28 -- Subject: viaWWW:Position for confocal microscopist 23, 28 -- References: {201408261107.s7QB7G0Y025594-at-ns.microscopy.com} 23, 28 -- In-Reply-To: {201408261107.s7QB7G0Y025594-at-ns.microscopy.com} 23, 28 -- X-Forwarded-Message-Id: {201408261107.s7QB7G0Y025594-at-ns.microscopy.com} 23, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 23, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Dear All, anybody out who can give me a detailed protocol how to grow / fix / postfix leucocytes / T-cells on coverslips to be imaged in the SEM? Best would be to have them OsO4-stained also at some point... Drying would be through ethanol to critical point drying.
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
What I used for FE-SEM of lymphocytes: Cultured cells on glass coverslips in their normal growth medium. Make *sure* they're a monolayer, nonconfluent is preferred. It's best to sputter-coat the coverslips first, then sterilize with alcohol or UV. The cells like a gold or gold/palladium substrate, and they're on a conductive surface, which makes life easier.
Fix 1-2 hrs at room temperature in 1.25% glutaraldehyde in appropriate buffer. The buffer weaseling is because the choice can depend on what you want to do with the cells. Sorenson's phosphate works, so does Na-cacodylate, HEPES, etc. I used 0.1M, 0.15M might work for yours. Add 1% monomeric tannic acid to the fix. This helps prevent holes in the cell membranes.
OsO4 wasn't needed, but if you want to use it, then 1% OsO4 + 1% monomeric tannic acid for 1-2 hours at room temp. Overnight in the 'frig would probably be OK, but I'd test it first.
Dehydrate through ethanol - I started at 30%, then 50-70-80-90-95-3x100%, 5 minutes each. CPD: 5 soaks at 3 minutes each in liquid CO2, then do the run. 5 minute soaks can also be used.
Coat with your favorite high-resolution coater.
Phil
} Dear All, } anybody out who can give me a detailed protocol how to grow / fix / postfix leucocytes / T-cells on coverslips to be imaged in the } SEM? } Best would be to have them OsO4-stained also at some point... } Drying would be through ethanol to critical point drying. } } Thanks, } Stefan }
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 9, 32 -- From oshel1pe-at-cmich.edu Tue Aug 26 11:33:59 2014 9, 32 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7QGXxrX008892 9, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Aug 2014 11:33:59 -0500 9, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 9, 32 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s7QGXvfS007515 9, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Aug 2014 12:33:57 -0400 9, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f8c::8dd1:f8c) by 9, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 9, 32 -- (TLS) id 14.3.181.6; Tue, 26 Aug 2014 12:33:58 -0400 9, 32 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 9, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 9, 32 -- 14.3.181.6; Tue, 26 Aug 2014 12:33:57 -0400 9, 32 -- Message-ID: {53FCB6F5.3090803-at-cmich.edu} 9, 32 -- Date: Tue, 26 Aug 2014 12:33:57 -0400 9, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 9, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 9, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 9, 32 -- MIME-Version: 1.0 9, 32 -- To: micro {Microscopy-at-microscopy.com} 9, 32 -- Subject: Re: [Microscopy] Fix leucocytes on coverslips 9, 32 -- References: {201408261602.s7QG2181005027-at-ns.microscopy.com} 9, 32 -- In-Reply-To: {201408261602.s7QG2181005027-at-ns.microscopy.com} 9, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 9, 32 -- Content-Transfer-Encoding: 7bit 9, 32 -- X-Originating-IP: [141.209.160.122] 9, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 9, 32 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 9, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 9, 32 -- X-CanItPRO-Stream: default 9, 32 -- X-Canit-Stats-ID: 05MHsxV3i - d279b6667ade - 20140826 9, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biology Center
The New York Structural Biology Center (NYSBC) seeks an experienced electron microscopist to join the staff of its Cryo-Electron Microscope Facility (http://cryoem.nysbc.org). The NYSBC is a shared center that supports state-of-the-art research in cryo-EM, NMR, and X-ray crystallography. Cryo-EM facilities include four transmission electron microscopes and a dual-beam scanning electron microscope, which support projects involving electron tomography, single particle analysis and electron crystallography of both stained and frozen-hydrated samples. Projects focus on 3D reconstruction of biological assemblies ranging from the atomic structure of membrane proteins, to the subunit organization in macromolecular complexes and the cellular anatomy of developing organisms, to 3D reconstructions of entire cells and their distributions within their native tissue using the dual-beam microscope. To assist in these developments, NYSBC seeks an individual with postdoctoral experience in biological electron microscopy and image reconstruction. This individual will carry out experiments in support of collaborative projects with affiliated investigators and will also have opportunities to pursue independent research projects. The individual should be capable of multitasking, should enjoy working with other people, should have a good working knowledge of electron microscopes and a strong research background. Good communication skills are essential.
To apply, please send a c.v. and the names of three references to rice-at-nysbc.org
-- William J. Rice, Ph.D. Senior Scientist, EM Facility Manager New York Structural Biology Center 89 Convent Avenue, NY, NY 10027
==============================Original Headers============================== 6, 21 -- From rice-at-nysbc.org Tue Aug 26 15:32:52 2014 6, 21 -- Received: from nysbc.org (mail1.nysbc.org [207.239.59.4]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7QKWqvI032527 6, 21 -- for {Microscopy-at-Microscopy.Com} ; Tue, 26 Aug 2014 15:32:52 -0500 6, 21 -- X-Footer: bnlzYmMub3Jn 6, 21 -- Received: from cem11.nysbc.org ([192.168.5.111]) 6, 21 -- (authenticated user rice-at-nysbc.org) 6, 21 -- by nysbc.org (Kerio Connect 8.2.4) 6, 21 -- for Microscopy-at-Microscopy.Com; 6, 21 -- Tue, 26 Aug 2014 16:32:50 -0400 6, 21 -- Message-ID: {53FCEEF2.2060005-at-nysbc.org} 6, 21 -- Date: Tue, 26 Aug 2014 16:32:50 -0400 6, 21 -- From: William Rice {rice-at-nysbc.org} 6, 21 -- Reply-To: rice-at-nysbc.org 6, 21 -- Organization: New York Structural Biology Center 6, 21 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 6, 21 -- MIME-Version: 1.0 6, 21 -- To: Microscopy-at-Microscopy.Com 6, 21 -- Subject: Research Scientist Position at the New York Structural Biology Center 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From waksobge-at-topgunner.com Wed Aug 27 00:48:29 2014 Return-Path: {waksobge-at-topgunner.com} Received: from smtp3.topgunner.com (95.58.41.85.megaline.telecom.kz [95.58.41.85] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7R5mR3M025452 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 27 Aug 2014 00:48:28 -0500 Message-ID: {E3843BD4.4239005-at-topgunner.com}
Let me ask a question about "stopping power." I have some unstained, thin sections of PVC mixed with nitrile rubber which I examined at 80KV in our TEM.
I would expect the chlorine containing PVC to be more opaque or have greater contrast than the nitrile, but the c triple bond N has me a little concerned about my judgment.
Does anyone have any experience with pvc/nitril morphology they would like to share?
Thanks! Frank ARDL
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 12, 28 -- From frank_karl-at-ardl.com Wed Aug 27 13:41:56 2014 12, 28 -- Received: from cal1-mh676b.smtproutes.com (cal1-mh676b.smtproutes.com [208.70.89.135]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7RIfrCl004872 12, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Aug 2014 13:41:54 -0500 12, 28 -- X-Katharion-ID: 1409164899.25543.cal1-mh676 12, 28 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by 12, 28 -- cal1-mh676.smtproutes.com [(192.69.16.48)] with ESMTP via TCP 12, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 27 Aug 2014 18:41:39 +0000 12, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 12, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Wed, 27 12, 28 -- Aug 2014 14:41:29 -0400 12, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 12, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 12, 28 -- {microscopy-at-microscopy.com} 12, 28 -- Date: Wed, 27 Aug 2014 14:41:28 -0400 12, 28 -- Subject: polymer phase morphology 12, 28 -- Thread-Topic: polymer phase morphology 12, 28 -- Thread-Index: Ac/CJoNrGsHc4AmaT+eOHNAtoOHaYA== 12, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C22B44EB5-at-exchange2k7.ad.ardl.com} 12, 28 -- Accept-Language: en-US 12, 28 -- Content-Language: en-US 12, 28 -- X-MS-Has-Attach: 12, 28 -- X-MS-TNEF-Correlator: 12, 28 -- acceptlanguage: en-US 12, 28 -- Content-Type: text/plain; charset="us-ascii" 12, 28 -- MIME-Version: 1.0 12, 28 -- Content-Transfer-Encoding: 8bit 12, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7RIfrCl004872 ==============================End of - Headers==============================
From drugstore_cheapest9-at-sv3.net.ua Wed Aug 27 21:10:08 2014 Return-Path: {drugstore_cheapest9-at-sv3.net.ua} Received: from 91-241-148-57-dyn.sv3.net.ua ([93.171.209.131]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7S2A6Gt029982 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 27 Aug 2014 21:10:06 -0500 Message-Id: {201408280210.s7S2A6Gt029982-at-ns.microscopy.com}
X-from: robernal-at-u.northwestern.edu ()
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Title-Subject: [Filtered] Z-axis orientation in JEOL 2100
Message: Hello All,
What is the positive z-axis in the stage of the JEOL 2100? Is it pointing up towards the electron gun, or down to the phosphor screen?
When I put a sample in my custom made TEM holder, it gets focused in the -90um z coordinate, which is too far; I want to know if I need to thin my sample support in order to bring the sample down (towards the phosphor screen), or add a spacer to bring it up (towards the electron gun).
Thanks.
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 27 23:43:23 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7S4hNTK030888 14, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Aug 2014 23:43:23 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id A7A1974C3FB 14, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Aug 2014 23:43:23 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id a+76jBGgRDH8 for {microscopy-at-microscopy.com} ; 14, 28 -- Wed, 27 Aug 2014 23:43:19 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id B183574C3ED 14, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Aug 2014 23:43:19 -0500 (CDT) 14, 28 -- Message-ID: {53FEB367.4070409-at-microscopy.com} 14, 28 -- Date: Wed, 27 Aug 2014 23:43:19 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Z-axis orientation in JEOL 2100 14, 28 -- References: {201408272049.s7RKn4F2026240-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201408272049.s7RKn4F2026240-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201408272049.s7RKn4F2026240-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
we are the proud owners of a Zeiss axioimager D1 coupled to a digital camera whose make I won't cite. This system is 10 years old and the camera is not working as well as we wish. The microscope cannot be the culprit because it is made in Germany ;-). The user needs to work several more years to pay the rest of his loan and cannot afford to be replaced. So I think it is time to change the camera (scientists are pragmatic). We don't need the super-high resolution last-model camera, it is mainly used to take pictures of agar plates for documentation (bright field). I wondered if we couldn't just couple a low-budget digital camera from the usual store around the corner (80-100 Euros). Did someone already try that? As a biologist I have nearly no knowledge in mechanics and I am not very experienced in photography neither (except microphotography, which doesn't help here). I wonder if it is possible to couple a usual digital camera (not a microscope camera) to a Zeiss microscope of this type? The camera should have a USB port of course and maybe there would be some ring on the market to couple both together in perfect hamony (a little bit like ebony and ivory). Please be kind enough to write in standard units, inches and gallons give me headaches. Pixels and megabits are OK ;-). Many thanks in advance.
Stephane
==============================Original Headers============================== 4, 34 -- From nizets2-at-yahoo.com Thu Aug 28 01:34:19 2014 4, 34 -- Received: from nm47.bullet.mail.ne1.yahoo.com (nm47.bullet.mail.ne1.yahoo.com [98.138.120.54]) 4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7S6YIWI018305 4, 34 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 01:34:19 -0500 4, 34 -- Received: from [127.0.0.1] by nm47.bullet.mail.ne1.yahoo.com with NNFMP; 28 Aug 2014 06:34:18 -0000 4, 34 -- Received: from [98.138.226.179] by nm47.bullet.mail.ne1.yahoo.com with NNFMP; 28 Aug 2014 06:31:18 -0000 4, 34 -- Received: from [66.196.81.173] by tm14.bullet.mail.ne1.yahoo.com with NNFMP; 28 Aug 2014 06:31:18 -0000 4, 34 -- Received: from [98.139.212.197] by tm19.bullet.mail.bf1.yahoo.com with NNFMP; 28 Aug 2014 06:31:17 -0000 4, 34 -- Received: from [127.0.0.1] by omp1006.mail.bf1.yahoo.com with NNFMP; 28 Aug 2014 06:31:17 -0000 4, 34 -- X-Yahoo-Newman-Property: ymail-4 4, 34 -- X-Yahoo-Newman-Id: 878458.57046.bm-at-omp1006.mail.bf1.yahoo.com 4, 34 -- Received: (qmail 12266 invoked by uid 60001); 28 Aug 2014 06:31:17 -0000 4, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1409207477; bh=TH3D5fy/C0kYUL0QKNwE9lQVvSVr/TRIJJnQuAHUOHc=; h=Message-ID:Date:From:Subject:To:MIME-Version:Content-Type; b=m9mDLedze6I85/BcbxB40bXDZb2RH8JSy87QgfkZjsvDV6ThJIRkd7MKsbpnXS0KZFU84CmzD3vT+Jdk+U01qEdk6yqc2uVLdpE5Tz02ycQKsSC5JEIciOZGC5jcg1ed0xZ6oWtsMogwVliux557jIaU2PUm9RcduWbdjxigEJI= 4, 34 -- X-YMail-OSG: K2JrkO4VM1kJlDsq_3n8E4rHeW84Vtca6u4IufzTGLiSvxf 4, 34 -- XdP.l3FYQ8GalfJO.JAGkyUUwMHVMgIsJujxloNv80mpRG7LHTCKTZD.0DtF 4, 34 -- YUKwwvZIIYSs31ly13h4jXme.XKfSqYwh6w2FtqnjaFQzPP7w88kDexB_zlW 4, 34 -- TtEO8.DnQM4IlDvdjc68Zlh2RYr9Iikiv22VM2X_5JjMRvry_6b5pLm_uVJi 4, 34 -- gqqbr_f_7LXSz9MTGU9fSpznElsAEqQQVxRL_KSbcoH0ZPjl0cnQmcJ6bS35 4, 34 -- xGFizgRP7AZgw2beZAjypIA9TO20xdK1Nj_bAP7bKVak9m8fqs8z1xcYU3HR 4, 34 -- TK2F1COeoVm9ZywKEc01cB7Q9adsloVcdEw1ZNOLHBLCfAuUkSOr7MyQo89c 4, 34 -- B4wed4PHVTn3dZrQ6L8WUR8AFMYh3bQXflE9OM3FTgATlW7t_0DljbVft4pC 4, 34 -- NJKjjvlgCf2nxytqPTYo8CBO3a3qk82Oet1G8V5YyPn1.nlXJlZHQmftjY7c 4, 34 -- NTyERV93DXYfMIeJwjCSj1oc.qOkpYj6fyLukvvfCyGy1wcZKOjIdlfjIhdY 4, 34 -- - 4, 34 -- Received: from [93.83.199.146] by web141702.mail.bf1.yahoo.com via HTTP; Wed, 27 Aug 2014 23:31:17 PDT 4, 34 -- X-Rocket-MIMEInfo: 002.001,RGVhciBjb2xsZWFndWVzLA0KDQp3ZSBhcmUgdGhlIHByb3VkIG93bmVycyBvZiBhIFplaXNzIGF4aW9pbWFnZXIgRDEgY291cGxlZCB0byBhIGRpZ2l0YWwgY2FtZXJhIHdob3NlIG1ha2UgSSB3b24ndCBjaXRlLg0KVGhpcyBzeXN0ZW0gaXMgMTAgeWVhcnMgb2xkIGFuZCB0aGUgY2FtZXJhIGlzIG5vdCB3b3JraW5nIGFzIHdlbGwgYXMgd2Ugd2lzaC4gVGhlIG1pY3Jvc2NvcGUgY2Fubm90IGJlIHRoZSBjdWxwcml0IGJlY2F1c2UgaXQgaXMgbWFkZSBpbiBHZXJtYW55IDstKS4gVGhlIHVzZXIgbmVlZHMgdG8BMAEBAQE- 4, 34 -- X-Mailer: YahooMailClassic/713 YahooMailWebService/0.8.203.696 4, 34 -- Message-ID: {1409207477.32063.YahooMailBasic-at-web141702.mail.bf1.yahoo.com} 4, 34 -- Date: Wed, 27 Aug 2014 23:31:17 -0700 4, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 4, 34 -- Subject: digital camera + Zeiss Axioimager D1 4, 34 -- To: microscopy-at-microscopy.com 4, 34 -- MIME-Version: 1.0 4, 34 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
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Email: mobradovic-at-vinca.rs Name: Marko Obradovic
Organization: INN "VINCA", Belgrade, Serbia
Title-Subject: [Filtered] Retrofiting TEM with a digital camera
Message: Hello,
My name is Marko and I am a PHD student from Vinca Institute, Serbia. This is my first message here, so I apologize in advance if I do something incorrectly. We have in our laboratory an EM400 Philips TEM, produced in 1980, which up until recently was used for material science characterization. It is currently being repaired and since we have some funds available I thought it could be useful to retrofit it with a digital camera, to make it easier to use. So, my question is the following: which bottom-mounted CCD camera would be the best Price-performance option for us, since the effects that we are looking fore are in 5 to 20 nm range? And also, are there any good used cameras for sale because our funds are somewhat limited?
Thank You all in advance,
Marko Obradovic Research Assistant, INN "VINCA", 11000 Belgrade, Serbia
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Aug 28 08:04:52 2014 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7SD4qRj009727 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:04:52 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id EFA1374C7BA 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:04:51 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id uneAW49DCfVw for {microscopy-at-microscopy.com} ; 18, 28 -- Thu, 28 Aug 2014 08:04:46 -0500 (CDT) 18, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id C2F8674C7AD 18, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:04:46 -0500 (CDT) 18, 28 -- Message-ID: {53FF28EE.8000902-at-microscopy.com} 18, 28 -- Date: Thu, 28 Aug 2014 08:04:46 -0500 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: viaWWW:Retrofiting TEM with a digital camera 18, 28 -- References: {201408280838.s7S8c2nf005991-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201408280838.s7S8c2nf005991-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201408280838.s7S8c2nf005991-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: kun.li-at-kaust.edu.sa Name: Kun Li
Organization: Imaging and Characterization Core Lab, King Abdullah University of Science and Technology
Title-Subject: [Filtered] Electron microscopy specialist (bio field)
Message: King Abdullah University of Science and Technology is an international graduate-level, merit-based research university located on the shores of the Red Sea in Saudi Arabia. Our state-of-the-art campus, globally renowned faculty and brand new facilities come together to provide the ideal setting for significant, high-impact research. KAUST is dedicated to inspiring a new age of scientific achievement in the Kingdom that will also benefit the region and the world.
Key responsibilities include: • Perform standardized protocols for histology • Handle research specimens based on sound knowledge of histological/immunochemical processes. • Bio TEM sample preparation and basic TEM imaging • Maintain and run EM preparation lab • Engage in KAUST research projects that require advanced electron microscopy techniques and provide value-added service
Requirement • Good knowledge of cell biology and cell structure • General understanding and expertise in cell/tissue fixation, processing, embedding, sectioning and staining. • Able to operate common sample preparation tools independently and able to use basic light microscopes. • Hands-on experience on EM sample preparation, conventional TEM sample preparation • Hands-on experience on Ultramicrotome • Working experience with cell culture • Good communication and inter-personal skills • A master’s degree in life science, or biochemistry; Ph. D degree holder can also be considered for higher position. • Minimum 2 years of relevant working experience • Fresh graduates with direct matching background and applicants with less working experience can be considered.
In addition to world class facilities and living conditions, you will be remunerated with a highly competitive package and the opportunity to work within an ambitious and growing University.
If interested, please contact kun.li-at-kaust.edu.sa
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Aug 28 08:06:34 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7SD6Yw2010784 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:06:34 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id 72C9B74C7CC 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:06:34 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id nZd5xmkLvOr5 for {microscopy-at-microscopy.com} ; 17, 28 -- Thu, 28 Aug 2014 08:06:29 -0500 (CDT) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 092F074C7BF 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:06:29 -0500 (CDT) 17, 28 -- Message-ID: {53FF2955.9000406-at-microscopy.com} 17, 28 -- Date: Thu, 28 Aug 2014 08:06:29 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: rviaWWW: Position Open Electron microscopy specialist (bio field) 17, 28 -- References: {201408281126.s7SBQVob008189-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201408281126.s7SBQVob008189-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201408281126.s7SBQVob008189-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 17, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: kun.li-at-kaust.edu.sa Name: Kun Li
Organization: King Abdullah University of Science and Technology
Title-Subject: [Filtered] Opening for TEM scientist
Message: King Abdullah University of Science and Technology is an international graduate-level, merit-based research university located on the shores of the Red Sea in Saudi Arabia. Our state-of-the-art campus, globally renowned faculty and brand new facilities come together to provide the ideal setting for significant, high-impact research. KAUST is dedicated to inspiring a new age of scientific achievement in the Kingdom that will also benefit the region and the world.
Key responsibilities include:
• New TEM technique/methodology/ analysis protocol development to meet the characterization challenges in a multi-user environment. • Advanced TEM applications (Cs corrected TEM/STEM, monochromized TEM/STEM, simulation) to solve challenging scientific problems • Actively engage in KAUST research projects that have high demand in TEM expertise • Conduct advanced TEM user training and EM lectures in faculty courses if needed. • Coach junior staffs in the group to improve their understanding and skills in TEM • Provide EM technique consultation to users to help them better plan and design their experiment • Work closely with service engineers to ensure a high equipment uptime
Requirement: • Strong theoretical background in electron microscopy • Good understanding of electron optics • Good knowledge of crystallography • Strong expertise in HR TEM/STEM imaging, electron diffraction, EDS and EELS; expertise in electron tomography and/or holography is a plus. • Experienced in microscope alignment • Strong knowledge of TEM/STEM simulation and familiar with some of the most commonly used simulation software, such as JEMS and/or MacTempas. • Good communication and inter-personal skills • A team player • A Ph. D degree in materials science, physics, chemistry, or closely related field; master degree holders with very good matching experience can also be considered.
In addition to world class facilities and living conditions, you will be remunerated with a highly competitive package and the opportunity to work within an ambitious and growing University.
If interested, please send your contact kun.li-at-kaust.edu.sa
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Aug 28 08:09:30 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7SD9UOl013821 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:09:30 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id 9707874C7E0 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:09:30 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id GadWZYNzDVzQ for {microscopy-at-microscopy.com} ; 17, 28 -- Thu, 28 Aug 2014 08:09:02 -0500 (CDT) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 3DE7F74C7D1 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:09:02 -0500 (CDT) 17, 28 -- Message-ID: {53FF29EE.8000101-at-microscopy.com} 17, 28 -- Date: Thu, 28 Aug 2014 08:09:02 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW: Position Open #2 TEM scientist 17, 28 -- References: {201408281205.s7SC5vlK008515-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201408281205.s7SC5vlK008515-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201408281205.s7SC5vlK008515-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 17, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: kun.li-at-kaust.edu.sa Name: Kun Li
Organization: King Abdullah University of Science and Technology
Title-Subject: [Filtered] Opening for TEM service engineer
Message: King Abdullah University of Science and Technology is an international graduate-level, merit-based research university located on the shores of the Red Sea in Saudi Arabia. Our state-of-the-art campus, globally renowned faculty and brand new facilities come together to provide the ideal setting for significant, high-impact research. KAUST is dedicated to inspiring a new age of scientific achievement in the Kingdom that will also benefit the region and the world.
Key responsibilities include: • Responsible for the overall maintenance of all the transmission electron microscopes in the Imaging and Characterization Core Lab to ensure high equipment uptime. • Conduct preventive maintenance of all the seven TEMs per predefined PM schedule. • Conduct trouble-shooting and corrective maintenance of all the TEMs. • Work with FEI factory service support team to speed up the trouble shooting and problem solving process. • Manage all the TEM spare parts and consumables. • Responsible for the management and maintenance of the TEM Sample Preparation Lab.
Requirement: • Good knowledge of working principle and maintenance of TEM • Able to perform basic TEM trouble shooting independently, such as compustage repairing and Lab6/W/FEG tip replacement. Knowledge of Cs corrector and monochromator service will be a plus. • Working knowledge of EDS and GIF will be advantageous. • A dedicated and results-oriented team player. • Minimum a bachelor’s degree in engineering, preferably in electrical/electronics-related field. • Minimum 7 years of relevant working experience for bachelor degree holders and 5 years of relevant working experience for master degree holders.
In addition to world class facilities and living conditions, you will be remunerated with a highly competitive package and the opportunity to work within an ambitious and growing University.
If interested , please contact kun.li-at-kaust.edu.sa
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==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Aug 28 08:10:14 2014 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7SDADH6015387 19, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:10:13 -0500 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 8E33674C7EE 19, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:10:13 -0500 (CDT) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id z9YYwin8RrAW for {microscopy-at-microscopy.com} ; 19, 28 -- Thu, 28 Aug 2014 08:10:10 -0500 (CDT) 19, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id D09DA74C7E1 19, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 08:10:10 -0500 (CDT) 19, 28 -- Message-ID: {53FF2A32.6030500-at-microscopy.com} 19, 28 -- Date: Thu, 28 Aug 2014 08:10:10 -0500 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: viaWWW:Position Open #3 TEM service engineer 19, 28 -- References: {201408281221.s7SCLWpp008862-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201408281221.s7SCLWpp008862-at-ns.microscopy.com} 19, 28 -- X-Forwarded-Message-Id: {201408281221.s7SCLWpp008862-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 19, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From wnibedu-at-gtmc.net Thu Aug 28 08:30:36 2014 Return-Path: {wnibedu-at-gtmc.net} Received: from 74-51-130-152.gtmc.net (74-51-130-152.gtmc.net [74.51.130.152]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7SDUaGK014439 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 28 Aug 2014 08:30:36 -0500 Message-ID: {810CCCA6.5462032-at-gtmc.net}
Rodrigo-
As far as I can tell, the arrows on the Z buttons correspond to the actual directions of motion. So Z} 0 indicates that the sample is above the eucentric plane.
You can find the answer by checking the objective lens current needed to bring the sample into focus. Press STD FOCUS. Then turn the Focus knob until the sample is in focus. If you turned Focus clockwise, the OL is overfocused (current larger than the standard value), so the sample is below the eucentric height. This indicates that Z will have to be increased (sample raised) to bring the sample to the eucentric height.
Look at it this way: 1/p=1/f-1/q. We're not changing the image distance q. Start off with the sample in focus: If the focal length f is increased (OL current decreased), the object distance p has to be increased to bring the object into focus. Since the sample is above the OL, this corresponds to raising the sample (increasing Z).
You can check how the OL current changes when turning the Focus knob by looking at the Status/Lens Voltage dialog. But the convention seems to be that turning clockwise increases the OL current.
-Phil
P.S. I'd be interested to know more about your custom holder.
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, August 27, 2014 11:11 PM To: Ahrenkiel, Phil
X-from: robernal-at-u.northwestern.edu ()
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Title-Subject: [Filtered] Z-axis orientation in JEOL 2100
Message: Hello All,
What is the positive z-axis in the stage of the JEOL 2100? Is it pointing up towards the electron gun, or down to the phosphor screen?
When I put a sample in my custom made TEM holder, it gets focused in the -90um z coordinate, which is too far; I want to know if I need to thin my sample support in order to bring the sample down (towards the phosphor screen), or add a spacer to bring it up (towards the electron gun).
Thanks.
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 27 23:43:23 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7S4hNTK030888 14, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Aug 2014 23:43:23 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id A7A1974C3FB 14, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Aug 2014 23:43:23 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id a+76jBGgRDH8 for {microscopy-at-microscopy.com} ; 14, 28 -- Wed, 27 Aug 2014 23:43:19 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id B183574C3ED 14, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Aug 2014 23:43:19 -0500 (CDT) 14, 28 -- Message-ID: {53FEB367.4070409-at-microscopy.com} 14, 28 -- Date: Wed, 27 Aug 2014 23:43:19 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Z-axis orientation in JEOL 2100 14, 28 -- References: {201408272049.s7RKn4F2026240-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201408272049.s7RKn4F2026240-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201408272049.s7RKn4F2026240-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 27, 37 -- From Phil.Ahrenkiel-at-sdsmt.edu Thu Aug 28 11:12:30 2014 27, 37 -- Received: from silver.sdsmt.edu (silver.sdsmt.edu [151.159.1.1]) 27, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7SGCTWI024736 27, 37 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Aug 2014 11:12:30 -0500 27, 37 -- Received: from SDSMT-ex13.sdsmt.edu (sdsmt-ex13.sdsmt.edu [151.159.3.13]) 27, 37 -- by silver.sdsmt.edu (8.14.9/8.14.7) with ESMTP id s7SGCTuE031534 27, 37 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=OK) 27, 37 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Aug 2014 10:12:29 -0600 27, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sdsmt.edu; 27, 37 -- s=sdsmtdkim; t=1409242349; 27, 37 -- bh=aSY9ioOKbGxEbZyJyPmu6lwzCYBUVB+fiwHKYfTZftw=; 27, 37 -- h=From:To:Subject:Date; 27, 37 -- b=VEeXY4TjQD3c7zXPPex5hZpD2OEFUBoHgJUiiOIOCoH6W2LuWdK1Vrhdg1nHGBsUH 27, 37 -- gXwGKFSqZiQ209hLXUIkZjTC+6djjF5F5UQgkY2rNsMheYkdN7E7ZREPWgbUnU+xZf 27, 37 -- QMe0SYGbZmUHzmZNBnBttpdDI5NG9Ij3ErSIqFPI= 27, 37 -- Received: from SDSMT-ex13.sdsmt.edu (2607:f558:1000:f::6d) by 27, 37 -- SDSMT-ex13.sdsmt.edu (2607:f558:1000:f::6d) with Microsoft SMTP Server (TLS) 27, 37 -- id 15.0.913.22; Thu, 28 Aug 2014 10:12:28 -0600 27, 37 -- Received: from SDSMT-ex13.sdsmt.edu ([fe80::1492:416b:26a0:40e8]) by 27, 37 -- SDSMT-ex13.sdsmt.edu ([fe80::1492:416b:26a0:40e8%15]) with mapi id 27, 37 -- 15.00.0913.011; Thu, 28 Aug 2014 10:12:28 -0600 27, 37 -- From: "Ahrenkiel, Phil" {Phil.Ahrenkiel-at-sdsmt.edu} 27, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 27, 37 -- Subject: RE: [Microscopy] viaWWW:Z-axis orientation in JEOL 2100 27, 37 -- Thread-Topic: [Microscopy] viaWWW:Z-axis orientation in JEOL 2100 27, 37 -- Thread-Index: AQHPwn6HHgQREyP5RkuJXmgUyopepJvmI4Jg 27, 37 -- Date: Thu, 28 Aug 2014 16:12:28 +0000 27, 37 -- Message-ID: {d7297b20ec3643949b64ac98cca88fa3-at-SDSMT-ex13.sdsmt.edu} 27, 37 -- Accept-Language: en-US 27, 37 -- Content-Language: en-US 27, 37 -- X-MS-Has-Attach: 27, 37 -- X-MS-TNEF-Correlator: 27, 37 -- x-originating-ip: [2607:f558:1000:14:8354:c249:d1a5:933f] 27, 37 -- Content-Type: text/plain; charset="us-ascii" 27, 37 -- MIME-Version: 1.0 27, 37 -- Content-Transfer-Encoding: 8bit 27, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7SGCTWI024736 ==============================End of - Headers==============================
They all make good cameras, with somewhat different emphases on application. As well as the quality of the camera, you also need to consider support, both with regard to service and applications.
Can I suggest you contact:
MB Science Service Hodrusha Hamre 485 SK-96663 Hodrusha SLOVAKIA
A long time ago, I used to work with Manfred - he knows what he is talking about. They are a Gatan agent but might be able to help with used cameras and should be able to provide support.
Best regards,
Larry Stoter (Retired - https://www.linkedin.com/pub/larry-stoter/53/439/963)
On 28 Aug 2014, at 14:37, microscopylistserver-noreply-at-microscopy.com wrote:
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==============================Original Headers============================== 14, 36 -- From larry.stoter-at-gmail.com Thu Aug 28 11:13:10 2014 14, 36 -- Received: from mail-wi0-f172.google.com (mail-wi0-f172.google.com [209.85.212.172]) 14, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7SGDAVP025399 14, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 11:13:10 -0500 14, 36 -- Received: by mail-wi0-f172.google.com with SMTP id n3so7789024wiv.11 14, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 09:13:09 -0700 (PDT) 14, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 36 -- d=gmail.com; s=20120113; 14, 36 -- h=content-type:mime-version:subject:from:in-reply-to:date 14, 36 -- :content-transfer-encoding:message-id:references:to; 14, 36 -- bh=K+EKxs8WxmFjpvSbvJj4+07o/EhZWypEeKX2T1s2BK0=; 14, 36 -- b=zFHmBL4tWumHo5LVH47hUUhEUNknHd4HqERYD/XQFYxs4lrsYd+5HVaThEAKor9u+C 14, 36 -- YYg1GkfpMG5iYCq65/K2hAkTCEf9G5K4nqQdKv/VYjOXXfkTyHdqnJzmIQatQ1mQ+xz5 14, 36 -- raeu/2ThxrKMnTWfx5BmZvgn2VILfK8cPqNEm3ie3fOncJs1W7AZi6D8lJi0oVgU9Fww 14, 36 -- t/7enol+Ibw2hCa+jdADy5yh8I9NGg8yWAXe8OpJP4qibdmY9j/kL19ayq4LdGMhVTBg 14, 36 -- Q8ggEtJkSpWF8vWvxJp2Uil8zU/TNFCL5G6785iYmI/F8JPIqL9dXrIGuNBpuqK0WGxJ 14, 36 -- alEA== 14, 36 -- X-Received: by 10.194.6.101 with SMTP id z5mr6598313wjz.79.1409242389597; 14, 36 -- Thu, 28 Aug 2014 09:13:09 -0700 (PDT) 14, 36 -- Received: from [10.175.1.101] (118.13.189.80.dyn.plus.net. [80.189.13.118]) 14, 36 -- by mx.google.com with ESMTPSA id w20sm37102353wie.7.2014.08.28.09.13.08 14, 36 -- for {multiple recipients} 14, 36 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 14, 36 -- Thu, 28 Aug 2014 09:13:09 -0700 (PDT) 14, 36 -- Content-Type: text/plain; charset=us-ascii 14, 36 -- Mime-Version: 1.0 (Mac OS X Mail 7.3 \(1878.6\)) 14, 36 -- Subject: Re: [Microscopy] viaWWW:Retrofiting TEM with a digital camera 14, 36 -- From: Larry Stoter {larry.stoter-at-gmail.com} 14, 36 -- In-Reply-To: {201408281337.s7SDb6Ch031955-at-ns.microscopy.com} 14, 36 -- Date: Thu, 28 Aug 2014 17:13:07 +0100 14, 36 -- Message-Id: {6522DE22-888F-4F3B-8CF0-CC03FB309B44-at-gmail.com} 14, 36 -- References: {201408281337.s7SDb6Ch031955-at-ns.microscopy.com} 14, 36 -- To: MSA Microscopy List {Microscopy-at-microscopy.com} , mobradovic-at-vinca.rs 14, 36 -- X-Mailer: Apple Mail (2.1878.6) 14, 36 -- Content-Transfer-Encoding: 8bit 14, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s7SGDAVP025399 ==============================End of - Headers==============================
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Email: freth001-at-umn.edu Name: Chris Frethem
Organization: University of Minnesota Characterization Facility
Title-Subject: [Filtered] Humidity / frosting problems in cryoEM lab
Message: Our labs are contending with high humidity problems during cryoultramicrotomy, cryoTEM (& Viotrobot sample preps), cryoSEM, etc. Midwestern summer weather is a challenge. Colder weather much less so. Air supply is whole-building; i.e. common source & exhaust, with some local zone control via variable air volume valves. Facilities Management has lately been able to keep temperatures in 68-73F range; relative humidity mostly in 48%-60% range. Facilities Management requested that I seek ideas from other facilities to improve situation. I suspect we'd have to isolate the facility from rest of building; probably very expensive. Adding dehumidifiers would add heat & noise (vibrations) to rooms but seem pointless if all building air is shared. Likewise we must preserve good air quality, i.e sufficient turnover. Understood that proper sample techniques and instruments and instrument use are key factors; just looking for other ideas from those similarly challenged. Thank you.
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Aug 28 18:14:04 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7SNE4lu005987 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 18:14:04 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 9024874CCC0 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 18:14:04 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id jqiCCZycNIpl for {microscopy-at-microscopy.com} ; 13, 28 -- Thu, 28 Aug 2014 18:14:00 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 97AE974CCB3 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2014 18:14:00 -0500 (CDT) 13, 28 -- Message-ID: {53FFB7B8.90307-at-microscopy.com} 13, 28 -- Date: Thu, 28 Aug 2014 18:14:00 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Humidity / frosting problems in cryoEM lab 13, 28 -- References: {201408281738.s7SHcjIi000980-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201408281738.s7SHcjIi000980-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201408281738.s7SHcjIi000980-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I just read your message and thought about how my glass of ice water last night had so much water on the outside. What if you put several beakers of ice on the other side of the room or maybe in your sink, would enough water be removed from the air by condensation to help?
Years ago I had problems with summer humidity causing too many holes in my collodion films for the grids that I was attempting to coat. I found I had to make them beside a running area heater. Hot and dry! It worked fine for me then I needed the ice water. I do question it for cryoEM however.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Pat
Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491 connellyps-at-mail.nih.gov
} Our labs are contending with high humidity problems during cryoultramicrotomy, cryoTEM (& } Viotrobot sample preps), cryoSEM, etc. Midwestern summer weather is a challenge. Colder weather much } less so. } Air supply is whole-building; i.e. common source & exhaust, with some local zone control via } variable air volume valves. Facilities Management has lately been able to keep temperatures in } 68-73F range; relative humidity mostly in 48%-60% range.
I was once facing a similar problem, aggravated by the face that the health and safety inspector had demanded a humidifier (!) for the building.
The high rate of air exchange (8 times per hour, as far as I remember) in the lab precluded the use of mobile dehumidifier units and any other provisional solutions. In the end, a large, fixed dehumidifier was installed on the corridor in the incoming air supply of three rooms - cryopreparation, Polara, and another microscope. Yes, this unit IS noisy (hence on the corridor) and using a lot of energy, but it is worth it!
Please let me know if you need any further details like manufacturer.
Best regards,
Guenter
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
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Dear Chris, Our solution to temperature and humidity control for an aberration corrected STEM (though not cryo) is a dedicated, semiconductor industry type A/C unit. They can be small enough for just a room - though they do need their own closed cycle duct work. Temperature is very precise and maximum allowable humidity and air flow rate are set independently. Totally quiet and stable in the room, as they are mounted remotely. Does not need venting to the outside. It is a more expensive solution, perhaps, but not compared to the price of the microscope or your lost productivity. I can pass along more details if you are interested. Regards, Larry Scipioni
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Email: freth001-at-umn.edu Name: Chris Frethem
Organization: University of Minnesota Characterization Facility
Title-Subject: [Filtered] Humidity / frosting problems in cryoEM lab
Message: Our labs are contending with high humidity problems during cryoultramicrotomy, cryoTEM (& Viotrobot sample preps), cryoSEM, etc. Midwestern summer weather is a challenge. Colder weather much less so. Air supply is whole-building; i.e. common source & exhaust, with some local zone control via variable air volume valves. Facilities Management has lately been able to keep temperatures in 68-73F range; relative humidity mostly in 48%-60% range. Facilities Management requested that I seek ideas from other facilities to improve situation. I suspect we'd have to isolate the facility from rest of building; probably very expensive. Adding dehumidifiers would add heat & noise (vibrations) to rooms but seem pointless if all building air is shared. Likewise we must preserve good air quality, i.e sufficient turnover. Understood that proper sample techniques and instruments and instrument use are key factors; just looking for other ideas from those similarly challenged. Thank you.
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Email: brian.choi-at-parkafm.com Name: Brian Choi
Organization: Park Systems
Title-Subject: [Filtered] Tissue sample preparation for SEM
Message: Dear whom has the know-how,
I tried to acquire the surface morphology of large intestin with SEM. In the SEM image I obtaiend, the surface of large intestin tissue is fully covered with some stiky muscus so that I can barely see the specific surface pattern of intestin.
Is there any treatment method to get rid of the muscus on the surface?
I will be very pleased if you inform me any protocol for this.
Thank you very much
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Aug 29 07:56:07 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7TCu6LK026434 17, 28 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2014 07:56:06 -0500 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id E6ACF74D330 17, 28 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2014 07:56:06 -0500 (CDT) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id fpStP7GMDVzq for {microscopy-at-microscopy.com} ; 17, 28 -- Fri, 29 Aug 2014 07:56:02 -0500 (CDT) 17, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 223DD74D323 17, 28 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2014 07:56:02 -0500 (CDT) 17, 28 -- Message-ID: {54007862.20504-at-microscopy.com} 17, 28 -- Date: Fri, 29 Aug 2014 07:56:02 -0500 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Tissue sample preparation for SEM 17, 28 -- References: {201408290715.s7T7FabL016379-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201408290715.s7T7FabL016379-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201408290715.s7T7FabL016379-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
What systems are currently being made for ion-beam coating for high-resolution FE-SEM. I know of the Gatan coater, and South Bay Technologies (and the osmium coater from SPI). What else is there? Thanks. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 1, 30 -- From oshel1pe-at-cmich.edu Fri Aug 29 12:50:38 2014 1, 30 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 1, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7THob5x002759 1, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Aug 2014 12:50:38 -0500 1, 30 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 1, 30 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s7THoZU2018286 1, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Aug 2014 13:50:36 -0400 1, 30 -- Received: from cas1.central.cmich.local (2002:8dd1:f8c::8dd1:f8c) by 1, 30 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 1, 30 -- (TLS) id 14.3.181.6; Fri, 29 Aug 2014 13:50:37 -0400 1, 30 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 1, 30 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 1, 30 -- 14.3.181.6; Fri, 29 Aug 2014 13:50:36 -0400 1, 30 -- Message-ID: {5400BD6C.60508-at-cmich.edu} 1, 30 -- Date: Fri, 29 Aug 2014 13:50:36 -0400 1, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 1, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 1, 30 -- MIME-Version: 1.0 1, 30 -- To: micro {Microscopy-at-microscopy.com} 1, 30 -- Subject: ion-beam high-resolution coaters for FE-SEM 1, 30 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 1, 30 -- Content-Transfer-Encoding: 7bit 1, 30 -- X-Originating-IP: [141.209.160.122] 1, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 30 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 1, 30 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 1, 30 -- X-CanItPRO-Stream: default 1, 30 -- X-Canit-Stats-ID: 05MIFOAre - 823860565142 - 20140829 1, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Dear Listers, in our SEM the condenser polar pieces have a couple of O-rings each around their body. Given that above and below the whole condenser section O-rings are present around the column bore, I wonder if those O-rings around the bodies of condenser polar pieces may affect the "general" vacuum level, or if they are aimed to something else, for instance keeping cleaner a particular area of the polar piece external surface. Any idea? Thanks
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universitŕ Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
==============================Original Headers============================== 5, 20 -- From dcristofori-at-unive.it Sat Aug 30 02:29:58 2014 5, 20 -- Received: from orion.unive.it (orion.unive.it [157.138.1.25]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7U7TwEi006306 5, 20 -- for {Microscopy-at-microscopy.com} ; Sat, 30 Aug 2014 02:29:58 -0500 5, 20 -- Received: by orion.unive.it (Postfix, from userid 498) 5, 20 -- id 0E20642021; Sat, 30 Aug 2014 09:31:00 +0200 (CEST) 5, 20 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 5, 20 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 5, 20 -- (No client certificate requested) 5, 20 -- by orion.unive.it (Postfix) with ESMTPSA id EF5554201C 5, 20 -- for {Microscopy-at-microscopy.com} ; Sat, 30 Aug 2014 09:30:59 +0200 (CEST) 5, 20 -- Message-ID: {54017EF6.6000009-at-unive.it} 5, 20 -- Date: Sat, 30 Aug 2014 09:36:22 +0200 5, 20 -- From: Davide Cristofori {dcristofori-at-unive.it} 5, 20 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 5, 20 -- MIME-Version: 1.0 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- Subject: SEM: O-rings on condenser polar pieces 5, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 5, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From uzenebo-at-kubangsm.ru Sat Aug 30 03:47:03 2014 Return-Path: {uzenebo-at-kubangsm.ru} Received: from 95x153x168x232.kubangsm.ru ([91.205.131.126]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s7U8l2h5025982 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 30 Aug 2014 03:47:03 -0500 Message-ID: {CF548FCB.7739586-at-kubangsm.ru}
Dear Davide,
Your SEM may be a JEOL. This couple of O-rings was designed for vacuum only. When the current flow inside the lens coil this pole piece is strongly set in position by the resultant magnetic field. This position is fixed by the mechanical design of the top of the pole piece and the associated screws. When contamination occurs here it comes from inside (gun pipe for example) and from the pumping system the more often. It may be better to use a good grease for those O-rings and a small amount of it to avoid contamination from O-rings.
-- Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique ŕ balayage et microanalyse 2 rue de la Houssiničre BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
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Dear Listers, in our SEM the condenser polar pieces have a couple of O-rings each around their body. Given that above and below the whole condenser section O-rings are present around the column bore, I wonder if those O-rings around the bodies of condenser polar pieces may affect the "general" vacuum level, or if they are aimed to something else, for instance keeping cleaner a particular area of the polar piece external surface. Any idea? Thanks
a big THANK YOU for those who answered my question about cheap solutions to couple a camera to a Zeiss LM. A got several interesting Emails, one specially from James, who told me about his experience with a Touptek EM-510C camera. I searched the web for infos about this camera and found lots of positive opinions, in particular in a german amateur forum. It seems to suit my needs and I found the camera in Ebay and Amazon for an incredible $179 (around 130 Euros). It is definitely not a high-end camera, don't misunderstand me but I am not looking for one neither. It has a C-mount and a USB port and the software seems to be appreciated (free to download on the company website). I think it would be a mistake not to try it when you think an ELISA kit alone costs around 500 Euros. I have no financial interest in this product, I just thought it would be an interesting information for other people.
a big NOT-THANK YOU for the registered forum users who already sent me and who will send me an automatic out-of-office reply. This is not funny. Seriously.
Have a nice and productive camera/week/life (optimally all 3).
Stephane
==============================Original Headers============================== 5, 34 -- From nizets2-at-yahoo.com Mon Sep 1 03:01:08 2014 5, 34 -- Received: from nm32.bullet.mail.ne1.yahoo.com (nm32.bullet.mail.ne1.yahoo.com [98.138.229.25]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s818189O027338 5, 34 -- for {microscopy-at-microscopy.com} ; Mon, 1 Sep 2014 03:01:08 -0500 5, 34 -- Received: from [127.0.0.1] by nm32.bullet.mail.ne1.yahoo.com with NNFMP; 01 Sep 2014 08:01:08 -0000 5, 34 -- Received: from [98.138.101.132] by nm32.bullet.mail.ne1.yahoo.com with NNFMP; 01 Sep 2014 07:58:26 -0000 5, 34 -- Received: from [66.196.81.174] by tm20.bullet.mail.ne1.yahoo.com with NNFMP; 01 Sep 2014 07:58:26 -0000 5, 34 -- Received: from [98.139.212.244] by tm20.bullet.mail.bf1.yahoo.com with NNFMP; 01 Sep 2014 07:58:26 -0000 5, 34 -- Received: from [127.0.0.1] by omp1053.mail.bf1.yahoo.com with NNFMP; 01 Sep 2014 07:58:26 -0000 5, 34 -- X-Yahoo-Newman-Property: ymail-4 5, 34 -- X-Yahoo-Newman-Id: 219323.86257.bm-at-omp1053.mail.bf1.yahoo.com 5, 34 -- Received: (qmail 88155 invoked by uid 60001); 1 Sep 2014 07:58:26 -0000 5, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1409558306; bh=eMFY0bchD/oQYMjh59v66FBC/ADqEb6zvQAMXFoFDNM=; h=Message-ID:Date:From:Subject:To:MIME-Version:Content-Type; b=IdCsS5FQ3XJ2zJFUbAUmaTqPHQTKPeWQ/7r7B3bdfTCaDZOr4DlMaD0mKDX4X+YmiM/ZPgENOV8IgeUV3v8Z4lGl7v+MoIGUIoeVwFeyR7ZzD2XHOFgeapKtdmATYlh+GSLMIZGs/SDsPq2LAChKHjc92i7axfF6FNYVQxCzsxU= 5, 34 -- X-YMail-OSG: trFfXhYVM1n25qNg64SFwcENlJLzAwgM..wMzCIoSfU5eGj 5, 34 -- f1aay4.UE1HUyboDZcPrSKPDby4qoiGIZT7PEIwey_M8xzewTdboF0qANtQP 5, 34 -- ojgbjgXe_EelnX9VOx61NAcFX.xNsd3no86f22OdUXjsxzFzAJLoFKcpU04Q 5, 34 -- rQ9_WNI3bjXefFuSG5L_4Jtflca2.GCI9_osH_kIx2ekjmmOJlmqwNBiiyVF 5, 34 -- aw2gncq13D_bto.eEj2q7VkV7crzv4azpj6mlnCwH5oPrsxnYulECmUPvcWs 5, 34 -- ZNqlntVWrpe02sIR_.58msSzdl8Dpr_3XFYCeYjVPXxKHP_CGhCZWit.cuoK 5, 34 -- 0IXN2Ng87Nr3.oqKTpzd8iDyr_OQc.eE1AmJGG845D4vVX5BUc7HbEyueyHQ 5, 34 -- hGRPSUwHauPSkVfblPVZn4h2s.Pi2daGCIKGbTVidSxdYBDl_X4c4s9jz.HH 5, 34 -- xR4orgkuP6s32_TemV2CJA0GxBMWILuDGePRbz9Sk.ht5zDA1_KdgX76zQ81 5, 34 -- uqqPp4qvOO3UCMJub6XOkgoem2jcI41Yy77dA4zGCupeCpGlC1FNkoVo20HV 5, 34 -- IqA-- 5, 34 -- Received: from [93.83.199.146] by web141701.mail.bf1.yahoo.com via HTTP; Mon, 01 Sep 2014 00:58:26 PDT 5, 34 -- X-Rocket-MIMEInfo: 002.001,RGVhciBsaXN0ZXJzLA0KDQphIGJpZyBUSEFOSyBZT1UgZm9yIHRob3NlIHdobyBhbnN3ZXJlZCBteSBxdWVzdGlvbiBhYm91dCBjaGVhcCBzb2x1dGlvbnMgdG8gY291cGxlIGEgY2FtZXJhIHRvIGEgWmVpc3MgTE0uDQpBIGdvdCBzZXZlcmFsIGludGVyZXN0aW5nIEVtYWlscywgb25lIHNwZWNpYWxseSBmcm9tIEphbWVzLCB3aG8gdG9sZCBtZSBhYm91dCBoaXMgZXhwZXJpZW5jZSB3aXRoIGEgVG91cHRlayBFTS01MTBDIGNhbWVyYS4NCkkgc2VhcmNoZWQgdGhlIHdlYiBmb3IgaW5mb3MgYWJvdXQgdGhpcyABMAEBAQE- 5, 34 -- X-Mailer: YahooMailClassic/716 YahooMailWebService/0.8.203.696 5, 34 -- Message-ID: {1409558306.23816.YahooMailBasic-at-web141701.mail.bf1.yahoo.com} 5, 34 -- Date: Mon, 1 Sep 2014 00:58:26 -0700 5, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 34 -- Subject: LM cheap camera - thank yous and not-thank yous 5, 34 -- To: microscopy-at-microscopy.com 5, 34 -- MIME-Version: 1.0 5, 34 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
From dyvvawe-at-unitymediagroup.de Mon Sep 1 03:40:59 2014 Return-Path: {dyvvawe-at-unitymediagroup.de} Received: from ip-109-90-82-125.hsi11.unitymediagroup.de (ip-109-90-82-125.hsi11.unitymediagroup.de [109.90.82.125]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s818ew2Q014399 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 1 Sep 2014 03:40:58 -0500 Message-ID: {604C4126.3958274-at-unitymediagroup.de}
Dear All,
has anybody experience with the substitute of Kodak D-19 developer for Kodak microscopy film 4489? I bought a package from Plano (Ted Pella) for 1 gallon. But it is not clear to me if it is for preparing a stock solution or the working solution and is the handling exactly the same as for the old Kodak D-19? I really appreciate any comment.
Best regards, Anne Dr. Anne Heller AG Elektronenmikroskopie Institut für Botanik (210) Universität Hohenheim Postfach, 70593 Stuttgart Tel. 0711-45922180 Fax. 0711-45923355
The U.S. Department of Agriculture, Agricultural Research Service has a Full Time Permanent Biologist position GS 9/11 available for a transmission electron microscopy specialist with the Electron & Confocal Microscopy Unit in Beltsville, MD. The job announcement number is: CK-14-1181279-TW. See the web page for full details. https://www.usajobs.gov/GetJob/ViewDetails/378945700. Position is open Sept. 2 through Sept. 15, 2014.
The purpose of this position is to provide support in a core microscopy facility providing expertise in transmission electron microscopy (TEM). The position involves preparation of various organisms, tissues, and materials from plants, animals and microbes for observation in a TEM, operating a TEM to produce high quality images, and instruct and supervise scientists to use the TEM. The position is located in the Electron and Confocal Microscopy Unit within the Soybean Genomics and Improvement Laboratory at the Beltsville Agricultural Research Center whose general mission is to provide collaborative research studies using electron, confocal, and light microscopy techniques to study plants, animals and microbes through the development of high quality and high resolution images for publication of research. Go to: www.usajobs.gov
Gary R. Bauchan Ph. D Director, Electron & Confocal Microscopy Unit USDA-ARS 10300 Baltimore Ave. Bldg. 012, 5th St., BARC-West Beltsville, MD 20705 301-504-6649 gary.bauchan-at-ars.usda.gov
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==============================Original Headers============================== 9, 45 -- From Gary.Bauchan-at-ARS.USDA.GOV Tue Sep 2 06:46:52 2014 9, 45 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1blp0186.outbound.protection.outlook.com [207.46.163.186]) 9, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s82BkqTU010536 9, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Sep 2014 06:46:52 -0500 9, 45 -- Received: from CO2PR06CA012.namprd06.prod.outlook.com (10.141.242.12) by 9, 45 -- CO2PR06MB667.namprd06.prod.outlook.com (10.141.227.23) with Microsoft SMTP 9, 45 -- Server (TLS) id 15.0.1015.19; Tue, 2 Sep 2014 11:46:51 +0000 9, 45 -- Received: from BY2FFO11FD057.protection.gbl (2a01:111:f400:7c0c::116) by 9, 45 -- CO2PR06CA012.outlook.office365.com (2a01:111:e400:142a::12) with Microsoft 9, 45 -- SMTP Server (TLS) id 15.0.1019.16 via Frontend Transport; Tue, 2 Sep 2014 9, 45 -- 11:46:51 +0000 9, 45 -- Received: from mail.usda.gov (199.135.140.13) by 9, 45 -- BY2FFO11FD057.mail.protection.outlook.com (10.1.15.185) with Microsoft SMTP 9, 45 -- Server (TLS) id 15.0.1010.11 via Frontend Transport; Tue, 2 Sep 2014 11:46:50 9, 45 -- +0000 9, 45 -- Received: from 001FSN2MPN1-028.001f.mgd2.msft.net ([169.254.8.205]) by 9, 45 -- 001FSN2MMR1-003.001f.mgd2.msft.net ([199.135.140.13]) with mapi id 9, 45 -- 14.03.0195.002; Tue, 2 Sep 2014 11:46:46 +0000 9, 45 -- From: "Bauchan, Gary" {Gary.Bauchan-at-ARS.USDA.GOV} 9, 45 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 45 -- Subject: TEM Expert Job Opening: USDA-ARS, Beltsville, MD 9, 45 -- Thread-Topic: TEM Expert Job Opening: USDA-ARS, Beltsville, MD 9, 45 -- Thread-Index: Ac/Go4PJ2VaWEcygRreVz5Nxpb7EDw== 9, 45 -- Date: Tue, 2 Sep 2014 11:46:46 +0000 9, 45 -- Message-ID: {39184AD737E8B649BD79EB255A7B697F400FA7E9-at-001FSN2MPN1-028.001f.mgd2.msft.net} 9, 45 -- Accept-Language: en-US 9, 45 -- Content-Language: en-US 9, 45 -- X-MS-Has-Attach: 9, 45 -- X-MS-TNEF-Correlator: 9, 45 -- x-originating-ip: [199.133.25.212] 9, 45 -- Content-Type: text/plain; charset="us-ascii" 9, 45 -- MIME-Version: 1.0 9, 45 -- X-EOPAttributedMessage: 0 9, 45 -- X-Forefront-Antispam-Report: 9, 45 -- CIP:199.135.140.13;CTRY:US;IPV:NLI;EFV:NLI;SFV:NSPM;SFS:(6009001)(438002)(199003)(189002)(22756005)(66066001)(85852003)(97756001)(22746005)(77096002)(33656002)(92726001)(55846006)(95666004)(84676001)(90102001)(2351001)(20776003)(15974865002)(47776003)(2501002)(21056001)(79102001)(81342001)(85306004)(92566001)(83072002)(50466002)(46406003)(68736004)(46102001)(44976005)(229853001)(83322001)(31966008)(74662001)(74502001)(86362001)(81542001)(15975445006)(19580395003)(107886001)(6806004)(99396002)(76482001)(50986999)(4396001)(107046002)(97736001)(110136001)(87936001)(86146001)(80022001)(77982001)(2656002)(81156004)(106466001)(64706001)(54356999)(19580405001)(104016003)(23726002)(69596002)(79686002);DIR:OUT;SFP:;SCL:1;SRVR:CO2PR06MB667;H:mail.usda.gov;FPR:;MLV:sfv;PTR:InfoDomainNonexistent;A:1;MX:1;LANG:en; 9, 45 -- X-Microsoft-Antispam: BCL:0;PCL:0;RULEID:;UriScan:; 9, 45 -- X-Forefront-PRVS: 0322B4EDE1 9, 45 -- Received-SPF: Pass (protection.outlook.com: domain of ARS.USDA.GOV designates 9, 45 -- 199.135.140.13 as permitted sender) receiver=protection.outlook.com; 9, 45 -- client-ip=199.135.140.13; helo=mail.usda.gov; 9, 45 -- Authentication-Results: spf=pass (sender IP is 199.135.140.13) 9, 45 -- smtp.mailfrom=Gary.Bauchan-at-ARS.USDA.GOV; 9, 45 -- X-OriginatorOrg: ars.usda.gov 9, 45 -- Content-Transfer-Encoding: 8bit 9, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s82BkqTU010536 ==============================End of - Headers==============================
We use the replacement D-19 here. It seems to be easier to over-develop with the Photographer's Formulary version than with the Kodak D-19. But, it needs to be tested. Curiously, none of the EM supply companies I've spoken with have bothered to do this, they all say "it's a straight replacement, use it like you did Kodak D-19." Which is not unreasonable, given that the PF version is the same chemicals in the same proportions as the Koday version, but still ...
The replacement D-19 is compounded by Photographer's Formulary http://stores.photoformulary.com/formulary-substitute-d-19/
This is the same mixture as Kodak D-19, but packaged as separate components, which have to be mixed in a specific order when making the stock D-19 solution - directions are in the box.
Phil
} Dear All, } } has anybody experience with the substitute of Kodak D-19 developer for } Kodak microscopy film 4489? I bought a package from Plano (Ted Pella) } for 1 gallon. But it is not clear to me if it is for preparing a stock } solution or the working solution and is the handling exactly the same } as for the old Kodak D-19? I really appreciate any comment. } } Best regards, } Anne } Dr. Anne Heller } AG Elektronenmikroskopie } Institut für Botanik (210) } Universität Hohenheim } Postfach, 70593 Stuttgart } Tel. 0711-45922180 } Fax. 0711-45923355
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 32 -- From oshel1pe-at-cmich.edu Tue Sep 2 07:20:21 2014 7, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s82CKLke030339 7, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Sep 2014 07:20:21 -0500 7, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 7, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s82CKI8o009490; 7, 32 -- Tue, 2 Sep 2014 08:20:20 -0400 7, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f8c::8dd1:f8c) by 7, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 7, 32 -- (TLS) id 14.3.181.6; Tue, 2 Sep 2014 08:20:18 -0400 7, 32 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 7, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 7, 32 -- 14.3.181.6; Tue, 2 Sep 2014 08:20:18 -0400 7, 32 -- Message-ID: {5405B601.4050901-at-cmich.edu} 7, 32 -- Date: Tue, 2 Sep 2014 08:20:17 -0400 7, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 32 -- MIME-Version: 1.0 7, 32 -- To: {Anne.Heller-at-uni-hohenheim.de} , micro {Microscopy-at-microscopy.com} 7, 32 -- Subject: Re: [Microscopy] Kodak D-19 developer 7, 32 -- References: {201409020957.s829vXlu032499-at-ns.microscopy.com} 7, 32 -- In-Reply-To: {201409020957.s829vXlu032499-at-ns.microscopy.com} 7, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-Originating-IP: [141.209.160.122] 7, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 32 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 7, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 7, 32 -- X-CanItPRO-Stream: default 7, 32 -- X-Canit-Stats-ID: 02MKckkIG - b60966682e94 - 20140902 7, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Hooke College of Applied Sciences, located in Westmont, IL, is offering a Transmission Electron Microscopy short course October 7-9, 2014. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.
For further TEM training details and registration information, please follow the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax)
==============================Original Headers============================== 9, 42 -- From CGorman-at-hookecollege.com Tue Sep 2 15:12:15 2014 9, 42 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 9, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s82KCFcs030107 9, 42 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2014 15:12:15 -0500 9, 42 -- X-ASG-Debug-ID: 1409688732-07bbd46048da050001-4CH8be 9, 42 -- Received: from TMGEX1.tmg.mccrone.com ([192.168.101.73]) by spam.mccrone.com with ESMTP id IBipjWFe1OIVKo7U for {microscopy-at-microscopy.com} ; Tue, 02 Sep 2014 15:12:12 -0500 (CDT) 9, 42 -- X-Barracuda-Envelope-From: CGorman-at-hookecollege.com 9, 42 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by 9, 42 -- TMGEX1.tmg.mccrone.com (192.168.101.73) with Microsoft SMTP Server (TLS) id 9, 42 -- 15.0.913.22; Tue, 2 Sep 2014 15:12:11 -0500 9, 42 -- Received: from TMGEX1.tmg.mccrone.com ([fe80::cc0d:92d8:b3c7:8537]) by 9, 42 -- TMGEX1.tmg.mccrone.com ([fe80::d8ce:618:435a:28fe%16]) with mapi id 9, 42 -- 15.00.0913.011; Tue, 2 Sep 2014 15:12:11 -0500 9, 42 -- From: Christine Gorman {CGorman-at-hookecollege.com} 9, 42 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 42 -- Subject: Transmission Electron Microscopy Short Course 9, 42 -- Thread-Topic: Transmission Electron Microscopy Short Course 9, 42 -- X-ASG-Orig-Subj: Transmission Electron Microscopy Short Course 9, 42 -- Thread-Index: Ac/G6i2E1zJZ8sHcQt+ykXkXg2wbCw== 9, 42 -- Date: Tue, 2 Sep 2014 20:12:10 +0000 9, 42 -- Message-ID: {51e9c82f961442f18ca3766db1f42232-at-TMGEX1.tmg.mccrone.com} 9, 42 -- Accept-Language: en-US 9, 42 -- Content-Language: en-US 9, 42 -- X-MS-Has-Attach: 9, 42 -- X-MS-TNEF-Correlator: 9, 42 -- x-vipre-scanned: 002E2184007FFC002E22D1 9, 42 -- x-originating-ip: [192.168.101.154] 9, 42 -- Content-Type: text/plain; charset="us-ascii" 9, 42 -- MIME-Version: 1.0 9, 42 -- X-Barracuda-Connect: UNKNOWN[192.168.101.73] 9, 42 -- X-Barracuda-Start-Time: 1409688732 9, 42 -- X-Barracuda-URL: http://spam.mccrone.com:80/cgi-mod/mark.cgi 9, 42 -- X-Virus-Scanned: by bsmtpd at mccrone.com 9, 42 -- X-Barracuda-BRTS-Status: 1 9, 42 -- X-Barracuda-Spam-Score: 0.00 9, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 9, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.9106 9, 42 -- Rule breakdown below 9, 42 -- pts rule name description 9, 42 -- ---- ---------------------- -------------------------------------------------- 9, 42 -- Content-Transfer-Encoding: 8bit 9, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s82KCFcs030107 ==============================End of - Headers==============================
For those who are intersted (and have some time for that stuff), here is the recepee of the D19b :
Grammes for 1l final solution. To be diluated at 30-40 °C in distilled water to make a concentrated mother solution at 1/4 or 1/3. This mother solution will be kept in an air and light free bottle at { 18°C and diutated as wanted for use.
Génol (Métol, Rhodol, Elon) 2.2 Sulfite de Sodium 72 Hydroquinone 8.8 Carbonate de Sodium 48 Bromure de potassium 4
Sorry, it's with french names, but it should be easy to find the translations.
Hoping it can help someone !
Jacques
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Le 02/09/2014 11:55, Anne.Heller-at-uni-hohenheim.de a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear All, } } has anybody experience with the substitute of Kodak D-19 developer for } Kodak microscopy film 4489? I bought a package from Plano (Ted Pella) } for 1 gallon. But it is not clear to me if it is for preparing a stock } solution or the working solution and is the handling exactly the same } as for the old Kodak D-19? I really appreciate any comment. } } Best regards, } Anne } Dr. Anne Heller } AG Elektronenmikroskopie } Institut fĂĽr Botanik (210) } Universität Hohenheim } Postfach, 70593 Stuttgart } Tel. 0711-45922180 } Fax. 0711-45923355 } } } ==============================Original Headers============================== } 5, 30 -- From Anne.Heller-at-uni-hohenheim.de Tue Sep 2 04:41:08 2014 } 5, 30 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s829f8Y2013151 } 5, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Sep 2014 04:41:08 -0500 } 5, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/simple; d=uni-hohenheim.de; } 5, 30 -- h=content-transfer-encoding:content-disposition:mime-version } 5, 30 -- :content-type:content-type:user-agent:subject:subject:from:from } 5, 30 -- :message-id:date:date; s=main; t=1409650866; x=1411465267; bh=6J } 5, 30 -- 9jvBRbP/tLokWJ3D24Qe18np65RdvmHWzbFIbePxY=; b=UjSlFTJNNq9Rw0n2Bg } 5, 30 -- k/00vQlEC4qA4X4DulQJTqSqvLXgCeKHtjsEepO66MJUAb1d/foRIvUfsl3pQLQU } 5, 30 -- eAd0QDhpvi8M4FvvIbCPFsl51MEj9SyvEpMGxB5ijjnAdV80Vw+On9FiAJ8iNVIr } 5, 30 -- smNd7atWCc6xrwCZl9GbffSGI= } 5, 30 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de } 5, 30 -- Received: from webmail.rz.uni-hohenheim.de (mailbox3.rz.uni-hohenheim.de [144.41.4.59]) } 5, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP } 5, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Sep 2014 11:41:06 +0200 (CEST) } 5, 30 -- Received: from jatropha.botanik.uni-hohenheim.de } 5, 30 -- (jatropha.botanik.uni-hohenheim.de [144.41.33.113]) by } 5, 30 -- webmail.uni-hohenheim.de (Horde Framework) with HTTP; Tue, 02 Sep 2014 } 5, 30 -- 11:41:06 +0200 } 5, 30 -- Date: Tue, 02 Sep 2014 11:41:06 +0200 } 5, 30 -- Message-ID: {20140902114106.Horde.QiRVwelYjmGw0YYw1N4gQw7-at-webmail.uni-hohenheim.de} } 5, 30 -- From: "Dr. Anne Heller" {Anne.Heller-at-uni-hohenheim.de} } 5, 30 -- To: Microscopy-at-microscopy.com } 5, 30 -- Subject: Kodak D-19 developer } 5, 30 -- User-Agent: Internet Messaging Program (IMP) H5 (6.1.4) } 5, 30 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes } 5, 30 -- MIME-Version: 1.0 } 5, 30 -- Content-Disposition: inline } 5, 30 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Hello All, I have just sent a copy of original Kodak formula for D-19 to Anne. It was copied from over 40 years old Kodak leaflet delivered with autoradiography plates. If anyone is interested in I can provide a link to PDF file with it. We have been using it over 30 years with 1+2 dilution (standard contrast) or 1+1 dilution (high contrast) for Kodak 4489 or Agfa Scientia plan films exposed at 80 kV.
It slightly differs from Jacques formula for D-19b.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
From ueyqquje-at-kanzlei-schmoelz.at Wed Sep 3 12:02:16 2014 Return-Path: {ueyqquje-at-kanzlei-schmoelz.at} Received: from mail.kanzlei-schmoelz.at (mail.kanzlei-schmoelz.at [91.112.125.218] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s83H2F9s021645 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 3 Sep 2014 12:02:16 -0500 Message-ID: {1459F2F4.9337952-at-kanzlei-schmoelz.at}
Hello Phill, hello All, The link is:
{http://www2.biomed.cas.cz/~benada/D-19.pdf}
My best regards
Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Wed, 3 Sep 2014 09:22:32 -0400, Philip Oshel {oshel1pe-at-cmich.edu} wrote : } Oldrich, } } I would appreciate a link to this pdf, please. } Thanks. } } Phil } } On 09/03/2014 05:57 , benada-at-biomed.cas.cz wrote: } } } } Hello All, } } I have just sent a copy of original Kodak formula for D-19 to Anne. } } It was copied from over 40 years old Kodak leaflet delivered with } } autoradiography plates. If anyone is interested in I can provide a } } link to PDF file with it. We have been using it over 30 years with } } 1+2 dilution (standard contrast) or 1+1 dilution (high contrast) for } } Kodak 4489 or Agfa Scientia plan films exposed at 80 kV. } } } } It slightly differs from Jacques formula for D-19b. } } } } Best regards Oldrich } } } } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } (989) 774-3576
I asked me from time to time why the "b" on the name D19-b. What about the "D19-a" and here we have the answer.
But the differences between both are smale. They have the same genol/hydroquinone ratio, so the same developping caracteristic on grey levels and contrast rendering. As the D19 has less accelerator, more preserving agent and less "anti-voile" (what's the name ine english ?), it gives probably a less agressive developpement and is more tolerant on developping time variations.
In comparison the D76, which is for fine grain and low contraste, has 2g genol, 5g hydroquinone, 100 g sodium sulfite and no sodium carbonate.
Probably the evolution to the "b" version was made to gain some contrast and/or to need a shorter developping time.
Best regards
Jacques
Le 03/09/2014 19:47, benada-at-biomed.cas.cz a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Phill, hello All, } The link is: } } {http://www2.biomed.cas.cz/~benada/D-19.pdf} } } My best regards } } Oldrich }
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Hello Listservers, Is there anyone out there that still burns objective aperture strips for their TEMs? I plan to try this with a Denton DV 502A evaporator and an old Hitachi 7600 strip (not thin foil). My questions are: At what vacuum do you burn the aperture? How long do you burn the aperture and at what current?
The best way to heat up your aperture strip, is to mount it so that the aperture area rests between two pieces of platinum. Why this method, well using a platinum boat does not usually provide an even level of heat along the aperture area.
Increase the current slowly and the aperture itself will start to glow. Continue until the aperture is an orange-red, stop, and watch the dark contamination around the apertures gradually disappear. Many people will suggest white heat, but in my experience this has a high possibility of damaging the aperture shape after one or two cleaning sessions. Orange-red is enough to obtain a clean aperture, and this level of heat will work for a number of cleaning procedures before any noticeable distortion of the apertures themselves.
The current drawn will vary on the contact between the aperture and the platinum supports. Slowly increasing the current will ensure you do not supply too much current and damage the aperture strip.
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu] Sent: 04 September 2014 14:49 To: protrain-at-emcourses.com
Michael,
The higher the vacuum the better, but at least 10^-6. I just go to bright red hot or orangish then let it cool.
Phil
} Hello Listservers, } Is there anyone out there that still burns objective aperture strips for } their TEMs? I plan to try this with a Denton DV 502A evaporator and an old } Hitachi 7600 strip (not thin foil). } My questions are: At what vacuum do you burn the aperture? How long do you } burn the aperture and at what current? } } Thank you, } Michael Delannoy } -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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Hooke College of Applied Sciences, located in Westmont, IL, is offering a Transmission Electron Microscopy short course October 7-9, 2014. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.
For further TEM training details and registration information, please follow the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax)
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Would like to hear from academic science departments on the subject of instrument maintenance.
How are different classes of instruments handled? i.e. computers, optical microscopes, small lab tools like PH meters and ovens, and major lab tools SEM, Spectrometers
Do you have a dedicated person to perform maintenance or is it done by lab personnel?
Do you use contract support or internal university resources?
Is maintenance a line item in your operating budget? How is that calculated?
From drugstore-popular14-at-zt.ua Thu Sep 4 14:31:14 2014 Return-Path: {drugstore-popular14-at-zt.ua} Received: from stat.fastnet.zt.ua ([193.150.6.37]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s84JVAvM026542 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 4 Sep 2014 14:31:12 -0500 Message-Id: {201409041931.s84JVAvM026542-at-ns.microscopy.com}
X-from: k.jack-at-uq.edu.au ()
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both k.jack-at-uq.edu.au as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: k.jack-at-uq.edu.au Name: Kevin Jack
Organization: The University of Queensland, Australia
Title-Subject: [Filtered] Dual Beam SEM/FIB Engineer at the Centre for Microscopy and Microanalysis, The University of Queensland
Message: Please see the following details for a newly advertised position at the Centre for Microscopy and Microanalysis, The University of Queensland, Australia.
Full details of the position and the application process can be found at http://jobs.uq.edu.au/caw/en/job/496351/dual-beam-sem-engineer
The Centre for Microscopy and Microanalysis (CMM) has a world standard laboratory equipped with state-of-the-art instrumentation and techniques. The Centre has nineteen electron microscopes, two XRD units, one XPS unit and provides leading edge capability in micro structural analysis to the staff and students across a broad range of disciplines at the University of Queensland. In addition, the CMM provides training to clients in relevant techniques and in solving characterization problems in a wide range of industrial, environmental and biological processes.
The role:
This role is to manage and operate a dual-beam scanning electron microscope which will be located in the Centre for Microscopy and Microanalysis (CMM). This role involves a number of aspects including the training of new users in the proper and effective usage of the instrumentation, in the preparation of sample specimens for transmission electron microscopy; the production of high quality 3-dimensional (tomographic) images the measurement of analytical data and crystallographic data from freshly milled samples. Moreover, aiding clients in experimental design and in the interpretation of high quality data will be a highly significant aspect this management position.
The person:
Applicants should possess qualifications and/or experience in dual beam scanning electron microscopy and transmission electron microscopy and have the demonstrated ability to manage the technical operations and maintenance of the FIB/SEM and be committed to understanding and servicing the requirements of its user community. The applicant will be an enthusiastic and capable individual with excellent oral and written communication skills and be willing to take responsibility for the facility and to facilitate the research programs of the users.
Full details of the position and the application process can be found at http://jobs.uq.edu.au/caw/en/job/496351/dual-beam-sem-engineer
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Two quick thoughts: Just a reminder that MME offers customized maintenance courses for microscopy.
Also, I had a intriguing meeting with Judith LaCoste recently, at M&M, and we discussed the whole process of aligning and calibrating microscopes. It is worth having a discussion with her. You can reach her at jlacoste-at-miacellavie.com.
CAVEAT: MME offers customized on-site courses on a for-fee basis.
Best regards, Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through January 2015. Call us today for a free training evaluation.
At 09:07 AM 9/5/2014, rbeavers-at-mail.smu.edu wrote:
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We run a 5 day "Monitoring & Maintaining the Electron Microscope" Course which has been extremely successful around the world including the USA. By the word "microscopy" I assume you are talking about light microscopes?
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: bfoster-at-the-mip.com [mailto:bfoster-at-the-mip.com] Sent: 05 September 2014 18:13 To: protrain-at-emcourses.com
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From pfujupae-at-ertelecom.ru Sat Sep 6 08:23:25 2014 Return-Path: {pfujupae-at-ertelecom.ru} Received: from 5x166x30x173.dynamic.yar.ertelecom.ru (5x166x30x173.dynamic.yar.ertelecom.ru [5.166.30.173] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s86DNONN000845 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 6 Sep 2014 08:23:25 -0500 Message-ID: {D4437396.8506850-at-ertelecom.ru}
Dear Listers,
At UMD NISP Lab we are trying to restore and return back to operation diffusion-pumped Pelco PAC-1 Advanced Coater 9500, I am wondering if someone may have a copy of the manual that (s)he would be willing to share.
PDF is preferred, but I would pay costs of copying and mailing if that is the only way.
Thank you very much beforehand, -- Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
I was trying to resurrect 20 y.o. W - filament LEO 1455 VP-SEM and stumbled into apparent software problem: during the start-up system would progress to "Initializing EO DLL" and stop at 25% of the progress bar. There are no error messages, dongle is installed on parallel port, and fiber optic communication between PC and the tool seem to be operational.
Wondering if there are users of this instrument who have seen similar problem and may have any ideas. Any kind of manuals, either user-level or service/maintenance would also be appreciated, as after the repair installing filament and aligning the column would be the next adventure...
Thank you very much beforehand, -- Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
From tujyma-at-weekendcruiser.com Mon Sep 8 05:48:52 2014 Return-Path: {tujyma-at-weekendcruiser.com} Received: from mail1.weekendcruiser.com ([197.0.47.62]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s88Amowk028105 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 8 Sep 2014 05:48:51 -0500 Message-ID: {0F3A528F.1567477-at-weekendcruiser.com}
Hello Listservers,
Our EDAX Genesis system needs an update to windows 7.
The current offer from the supplier includes an unexpecedly expensive PC (} 3000 Euros). I'm wondering if there are alternatives to this scenario.
Is there anyone out with experience on this topic?
Best regards
Bettina
________________________________________________________________  Dipl. Ing. (FH) Bettina Wolpensinger  Abteilung Photovoltaik Photovoltaics Department  Elektronenmikroskopie Electron Microscopy Institut fĂĽr Solarenergieforschung GmbH Am Ohrberg 1 D-31860 Emmerthal Phone: +49 (0) 5151 999 313 (BĂĽro) oder 218 (Mikroskop) Fax: +49 (0) 5151 999 400 eMail: b.wolpensinger-at-isfh.de Internet: www.isfh.de Handelsregister: Amtsgericht Hannover HRB 100547 GeschäftsfĂĽhrer: Prof. Dr. Rolf Brendel Vorsitzender des Aufsichtsrats: Ministerialrat Dr. Hans Schroeder ________________________________________________________________   Â
==============================Original Headers============================== 9, 38 -- From wolpensinger-at-isfh.de Mon Sep 8 07:55:21 2014 9, 38 -- Received: from mo4-p05-ob.smtp.rzone.de (mo4-p05-ob.smtp.rzone.de [81.169.146.181]) 9, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s88CtKjX030258 9, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Sep 2014 07:55:20 -0500 9, 38 -- X-RZG-AUTH: :JX8CfWCtb/w60+AJo5e+PaCH7pGjy/wwSnyVwzvDM2lMSw7xXhO9S3iHxAWfMlk6EoE= 9, 38 -- X-RZG-CLASS-ID: mo05 9, 38 -- Received: from hermes.intern.isfh.de (static-090-153-016-010-teleos.ewe-ip-backbone.de [90.153.16.10]) 9, 38 -- by smtp.strato.de (RZmta 35.8 AUTH) 9, 38 -- with ESMTPA id y017d8q88CtJOAq 9, 38 -- for {Microscopy-at-microscopy.com} ; 9, 38 -- Mon, 8 Sep 2014 14:55:19 +0200 (CEST) 9, 38 -- Received: from localhost (hermes.intern.isfh.de [127.0.0.1]) 9, 38 -- by localhost (Postfix) with ESMTP id F339F57EA35; 9, 38 -- Mon, 8 Sep 2014 14:55:18 +0200 (CEST) 9, 38 -- X-Virus-Scanned: amavisd-new 9, 38 -- X-Spam-Flag: NO 9, 38 -- X-Spam-Score: -4.551 9, 38 -- X-Spam-Level: 9, 38 -- X-Spam-Status: No, score=-4.551 required=6 tests=[ALL_TRUSTED=-1, 9, 38 -- BAYES_00=-1.9, MIME_QP_LONG_LINE=0.001, RP_MATCHES_RCVD=-1.652] 9, 38 -- autolearn=ham 9, 38 -- Received: from hermes.intern.isfh.de ([127.0.0.1]) 9, 38 -- by localhost (hermes.intern.isfh.de [127.0.0.1]) (amavisd-new, port 10033) 9, 38 -- with ESMTP id gAFikg--UI23; Mon, 8 Sep 2014 14:55:15 +0200 (CEST) 9, 38 -- Received: from hermes.intern.isfh.de (hermes.intern.isfh.de [127.0.0.1]) 9, 38 -- by hermes.intern.isfh.de (Postfix) with ESMTP id B5CD157E209; 9, 38 -- Mon, 8 Sep 2014 14:55:15 +0200 (CEST) 9, 38 -- Subject: EDAX Software Update - high cost - Germany 9, 38 -- From: =?utf-8?Q?Bettina_Wolpensinger?= {wolpensinger-at-isfh.de} 9, 38 -- To: =?utf-8?Q?Microscopy=40microscopy=2Ecom?= {Microscopy-at-microscopy.com} 9, 38 -- Date: Mon, 8 Sep 2014 14:55:15 +0200 9, 38 -- Mime-Version: 1.0 9, 38 -- Content-Type: text/plain; charset=utf-8 9, 38 -- X-Priority: 3 (Normal) 9, 38 -- X-Mailer: Zarafa 7.1.10-44973 9, 38 -- Message-Id: {zarafa.540da733.4643.1930694e51ef0a32-at-hermes.intern.isfh.de} 9, 38 -- Content-Transfer-Encoding: 8bit 9, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s88CtKjX030258 ==============================End of - Headers==============================
From pharmacy_reasonable9-at-telecolumbus.net Mon Sep 8 11:17:18 2014 Return-Path: {pharmacy_reasonable9-at-telecolumbus.net} Received: from cable-82-119-4-6.cust.telecolumbus.net (cable-82-119-4-6.cust.telecolumbus.net [82.119.4.6]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s88GHD5T021693 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 8 Sep 2014 11:17:16 -0500 Message-Id: {201409081617.s88GHD5T021693-at-ns.microscopy.com}
X-from: rnichols-at-bcm.edu ()
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Email: rnichols-at-bcm.edu Name: ralph nichols
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Giudelines for TEM Digital Camera Use
Message: Hello Listers,
I hope all had a great summer. I'm about to get a digital camera for my TEM (YES!). This is a CORE facility. Some of our researchers have shied away from using it because of the film imaging. I anticipate more of them will be wanting to use the TEM. My questions for those whom manage TEM with digital cameras with multiple users: what kind of guidelines that I need to use in managing the camera such as should I restrict the kind of flash drives to down load the images and any other suggestions? Please email me directly to rnichols-at-bcm.edu.
Thanks
Ralph Nichols
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Following a recent upgrade of our cryo-TEM instruments, we are exploring the possibility of selling some of the auxiliary equipment previously serving the Tecnai F20 TEM (FEI company). All items detailed are performing (beyond) specifications and are in perfect working conditions.
The auxiliary equipment list includes:
· 1. One GIF 2002 Gatan’s 2nd generation post-column energy filter, equipped with cryo-sensitive 2K x 2K MSC CCD camera, controllers and a retractable TV-rate camera. The system has always been under service contract.
· 2. Two DH626 Single Tilt Liquid Nitrogen Cryo Transfer Holders, 60ş and 70ş, with transfer stations, Gatan Inc
· 3. One CT3500TR Single Tilt Rotation Liquid Nitrogen Cryo Transfer Holder, Transfer Station and Controller, Gatan Inc.
· 4. Two Advanced-Tomography Holders Model 2020, Fischione Instruments, Inc.
· 5. One: CompuStage Rotation Holder, Room Temperature, FEI company
AFMWorkshop's free livestreaming AFM Seminar Series continues tomorrow with a discussion led by Paul West, Ph.D., on AFM Probe-Cantilever Selection.
AFM operators must choose from a wide array of probe types when scanning samples. This seminar will cover the probe-cantilever types that work best for various types of samples and modes.
To Register, visit AFMWorkshop's UStream Channel: http://www.ustream.tv/channel/Atomic-Force-Microscopes. All participants are encouraged to ask questions live.
Thank you, Pamela Stone AFMWorkshop, Inc.
Pamela K. Stone AFMWorkshop, Inc. 1434 E. 33rd Street Signal Hill, CA 90755 (888)671-5539 (O) (562)274-2788 (M) www.afmworkshop.com
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Dear Listers,
Just a reminder about tomorrow's webinar:A Better Choice than Photoshop for Color Brightfield Microscopy
Dear All,
thank you for all who responded to my question. In the meantime I tested the substitute for Kodak D-19 from Ted Pello for 1 gallon. It gives 1 gallon stock solution. For Kodak plates 4489 4 min in 1+2 dilution the result is fine. It is a really a substitute for Kodak D-19.
Best regards, Anne Dr. Anne Heller AG Elektronenmikroskopie Institut für Botanik (210) Universität Hohenheim Postfach, 70593 Stuttgart Tel. 0711-45922180 Fax. 0711-45923355
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] EELS & EFTEM Analysis Training School
Message: EELS & EFTEM Analysis Training School Gatan, Inc. Pleasanton, California, USA October 14-17, 2014
Want to refresh or advance your EELS and EFTEM knowledge? Join us for an intensive 4-day training school that incorporates lectures, computer laboratories, and microscope practicals to provide participants with comprehensive, hands-on training on key EELS and EFTEM topics and technology.
Online registration is now open: http://ftp.gatan.com/training/formOct2014.php. School fee: $1,750.00 US Dollars. Space is limited.
Overview: Transmission electron microscopy (TEM) reveals details of natural and man-made structures at the micrometer, nanometer, and even sub-nanometer scale. Energy-filtered TEM (EFTEM) and electron energy-loss spectroscopy (EELS) are the ideal analytical partners to the high spatial resolution provided by TEM in both the conventional and scanned (STEM) imaging modes.
This course reviews the basic theory and practice of EELS imaging and analysis in the TEM, but its main emphasis is on practical techniques, optimum deployment of Gatan hardware and software systems, and advanced EELS and EFTEM applications. Some prior experience with EELS, EFTEM, and Gatan systems is recommended, as is a good familiarity with TEM/STEM instrumentation and techniques. By the end of the course, participants can expect to know how best to optimize the performance of their Gatan EELS hardware as well as their EELS and EFTEM experimental setups in order to capture and extract the maximum amount of information from their TEM samples.
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both twh-at-cen.dtu.dk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: twh-at-cen.dtu.dk Name: Thomas
Organization: DTU Cen
Title-Subject: [Filtered] Optical bench software
Message: Hi.
We just started up a course in electron microscopy (both TEM/STEM/SEM etc). For illustrating the effects of condenser lenses and apertures on the final probe in the SEM, it would be nice to have some optical bench software. Does anyone know of such software? Preferably freeware, but it doesn't have to be.
Thanks.
Thomas
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Message: NIBSC with JEOL (UK) Ltd. LEICA and Gatan are holding a dedicated cryo-workshop based on technologies installed at the NIBSC Imaging laboratory, Potters Bar, London, UK. The course will be held over 5 days commencing Monday 27th October through to Friday 31st October 2014. Delegate numbers will be strictly limited to ensure maximum hands-on training. The course will embrace cryo-preparation techniques using Cryo-FEGSEM, Cryo-TEM with GIF quantum and many associated preparation techniques: High pressure freezing (HMP010), Freeze fracture (Leica/BAF060), Cryo-sectioning, Freeze substitution, Vitreous thin films (plunge freezing), Tomography and High resolution Cryo-FEG SEM. This year new automated acquisition solutions will also be taught. The ethos of the course is very much “hands-on” with technical support lectures from scientists in these fields and application support from JEOL, LEICA and invited specialists from University of Lausanne and Max Plank Institute.
The course cost is ÂŁ550.00 including conference dinner and lunches but excluding accommodation.
Applications are invited, please include a brief description of your current interests to Gill Cathro Email: Gill.Cathro-at-nibsc.org; Tel: +44 (0) 1707 641561; Fax: +44 (0) 1707 641578
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From saetyqeu-at-ramnet.su Wed Sep 10 15:18:43 2014 Return-Path: {saetyqeu-at-ramnet.su} Received: from nat-10-90-124-0.ramnet.su (nat-10-90-124-0.ramnet.su [178.22.55.124] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8AKIf9n009006 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 10 Sep 2014 15:18:42 -0500 Message-ID: {3CC54FB7.2497180-at-ramnet.su}
Dear Colleagues,
WE are in the process of searching for alternative systems to replace our aging Toshiba UPS 1600XPs, we use them to backup our transmission electron microscopes. Can anyone suggest a comparable system that works for you?
Thank you very much in advance.
Xinran
________________________________________
Xinran Nick Liu, M.D. & Ph.D. Director of CCMI Electron Microscopy Core Facility Research Scientist in Cell Biology Yale University School of Medicine 333 Cedar Street, SHM I-E16A New Haven, CT 06520 Office: 203.785.4050 Lab: 203.785.5390 http://medicine.yale.edu/ccmi/em
==============================Original Headers============================== 10, 35 -- From xinran.liu-at-yale.edu Thu Sep 11 10:21:38 2014 10, 35 -- Received: from vm-emlprdomg-03.its.yale.edu (vm-emlprdomg-03.its.yale.edu [130.132.50.161]) 10, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8BFLccP026700 10, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Sep 2014 10:21:38 -0500 10, 35 -- Received: from x10-caht4.yu.yale.edu (x10-caht4.its.yale.edu [130.132.61.68]) 10, 35 -- by vm-emlprdomg-03.its.yale.edu (8.14.5/8.14.5) with ESMTP id s8BFLbo1020174 10, 35 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 10, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Sep 2014 11:21:37 -0400 10, 35 -- Received: from X10-MBX13.yu.yale.edu ([169.254.13.249]) by 10, 35 -- x10-caht4.yu.yale.edu ([130.132.61.68]) with mapi id 14.03.0174.001; Thu, 11 10, 35 -- Sep 2014 11:21:37 -0400 10, 35 -- From: "Liu, Xinran" {xinran.liu-at-yale.edu} 10, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 35 -- Subject: Power backup systems for TEM 10, 35 -- Thread-Topic: Power backup systems for TEM 10, 35 -- Thread-Index: AQHPzdQTteGcCmKiRESYaHBc2AACDQ== 10, 35 -- Date: Thu, 11 Sep 2014 15:21:36 +0000 10, 35 -- Message-ID: {D037363F.216B6%xinran.liu-at-yale.edu} 10, 35 -- Accept-Language: en-US 10, 35 -- Content-Language: en-US 10, 35 -- X-MS-Has-Attach: 10, 35 -- X-MS-TNEF-Correlator: 10, 35 -- user-agent: Microsoft-MacOutlook/14.4.3.140616 10, 35 -- x-originating-ip: [130.132.61.93] 10, 35 -- Content-Type: text/plain; charset="us-ascii" 10, 35 -- Content-ID: {E968EF25C5BCD34D907CB27865EEAD63-at-connect.yale.edu} 10, 35 -- MIME-Version: 1.0 10, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.12.52,1.0.28,0.0.0000 10, 35 -- definitions=2014-09-11_05:2014-09-11,2014-09-11,1970-01-01 signatures=0 10, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 10, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 10, 35 -- engine=7.0.1-1402240000 definitions=main-1409110129 10, 35 -- X-Yale-Not-Spam: For more info see: http://www.yale.edu/email/spam/content.html 10, 35 -- Content-Transfer-Encoding: 8bit 10, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s8BFLccP026700 ==============================End of - Headers==============================
From affordable_drugstore4-at-arcotoys.com Thu Sep 11 12:02:50 2014 Return-Path: {affordable_drugstore4-at-arcotoys.com} Received: from smtp3.arcotoys.com (221.216.87.109.triolan.net [109.87.216.221] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8BH2m2V014983 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 11 Sep 2014 12:02:49 -0500 Message-Id: {201409111702.s8BH2m2V014983-at-ns.microscopy.com}
Dear Xinran, At a past job, I tested and subsequently had installed UPS/power conditioning solutions from PowerVar. I found them to have good prices, small units, and a range of battery capacity options (we were looking protection against momentary power loss or for just a few minutes of run time for SEM's and FIB's, to allow for gracious shut down.) The units worked well and did in fact come into action more than once. Good luck!
-----Original Message----- X-from: xinran.liu-at-yale.edu [mailto:xinran.liu-at-yale.edu] Sent: Thursday, September 11, 2014 11:37 AM To: LES-at-ZSGENETICS.COM
Dear Colleagues,
WE are in the process of searching for alternative systems to replace our aging Toshiba UPS 1600XPs, we use them to backup our transmission electron microscopes. Can anyone suggest a comparable system that works for you?
Thank you very much in advance.
Xinran
________________________________________
Xinran Nick Liu, M.D. & Ph.D. Director of CCMI Electron Microscopy Core Facility Research Scientist in Cell Biology Yale University School of Medicine 333 Cedar Street, SHM I-E16A New Haven, CT 06520 Office: 203.785.4050 Lab: 203.785.5390 http://medicine.yale.edu/ccmi/em
==============================Original Headers============================== 10, 35 -- From xinran.liu-at-yale.edu Thu Sep 11 10:21:38 2014 10, 35 -- Received: from vm-emlprdomg-03.its.yale.edu (vm-emlprdomg-03.its.yale.edu [130.132.50.161]) 10, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8BFLccP026700 10, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Sep 2014 10:21:38 -0500 10, 35 -- Received: from x10-caht4.yu.yale.edu (x10-caht4.its.yale.edu [130.132.61.68]) 10, 35 -- by vm-emlprdomg-03.its.yale.edu (8.14.5/8.14.5) with ESMTP id s8BFLbo1020174 10, 35 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 10, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Sep 2014 11:21:37 -0400 10, 35 -- Received: from X10-MBX13.yu.yale.edu ([169.254.13.249]) by 10, 35 -- x10-caht4.yu.yale.edu ([130.132.61.68]) with mapi id 14.03.0174.001; Thu, 11 10, 35 -- Sep 2014 11:21:37 -0400 10, 35 -- From: "Liu, Xinran" {xinran.liu-at-yale.edu} 10, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 35 -- Subject: Power backup systems for TEM 10, 35 -- Thread-Topic: Power backup systems for TEM 10, 35 -- Thread-Index: AQHPzdQTteGcCmKiRESYaHBc2AACDQ== 10, 35 -- Date: Thu, 11 Sep 2014 15:21:36 +0000 10, 35 -- Message-ID: {D037363F.216B6%xinran.liu-at-yale.edu} 10, 35 -- Accept-Language: en-US 10, 35 -- Content-Language: en-US 10, 35 -- X-MS-Has-Attach: 10, 35 -- X-MS-TNEF-Correlator: 10, 35 -- user-agent: Microsoft-MacOutlook/14.4.3.140616 10, 35 -- x-originating-ip: [130.132.61.93] 10, 35 -- Content-Type: text/plain; charset="us-ascii" 10, 35 -- Content-ID: {E968EF25C5BCD34D907CB27865EEAD63-at-connect.yale.edu} 10, 35 -- MIME-Version: 1.0 10, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.12.52,1.0.28,0.0.0000 10, 35 -- definitions=2014-09-11_05:2014-09-11,2014-09-11,1970-01-01 signatures=0 10, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 10, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 10, 35 -- engine=7.0.1-1402240000 definitions=main-1409110129 10, 35 -- X-Yale-Not-Spam: For more info see: http://www.yale.edu/email/spam/content.html 10, 35 -- Content-Transfer-Encoding: 8bit 10, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s8BFLccP026700 ==============================End of - Headers==============================
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From wgesafou-at-rr.com Fri Sep 12 02:01:28 2014 Return-Path: {wgesafou-at-rr.com} Received: from cpe-75-83-26-72.socal.res.rr.com (cpe-75-83-26-72.socal.res.rr.com [75.83.26.72]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8C71KXa020913 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 12 Sep 2014 02:01:24 -0500 Message-ID: {84D3D3CE.6402468-at-rr.com}
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Email: anita.garg-1-at-nasa.gov Name: Anita Garg
Organization: NASA GRC/UT
Title-Subject: [Filtered] Service charge for TEM work
Message: Dear Listserver I would like to know per hour charge to (1) use an outside lab/university to do the TEM, (2) get the TEM done by another company/university. What are the time constraints and wait time? Thanks in advance.
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The University of Illinois Materials Research Lab is sponsoring its 3rd Annual Biostructures Conference.
New this year are student presentation and poster competitions.
One does not have to compete to have an informative time—two days of invited experts will be presenting engaging sessions:
Dr. Bill Wilson (Materials Research Lab): Devices to make proteins on the nano scale Dr. David Hall (Albert Einstein College of Medicine): High tech solutions for Biological samples/C. elegans. Dr. Sivaguru Mayandi (Institute for Genomic Biology): Confocal and the 3D shape of pollen. Dr. Adam Stern ( UI Veterinary Medicine): The art of histology. Linda Arseneau (UI Division of Research Safety): Biological safety Dr. James Nardi (UI Entomology): Preparing insects for microscopy Dr. Scott MacLaren (Materials Research Lab): Biological AFM Lou Ann Miller (Materials Research Lab): Biological TEM Dr. Stephan Lezmi (UI Veterinary Medicine): Immunohistochemistry JPK (a company that will demo instruments and speak): Biological AFM Mark Reitsma (Oxford-Asylum, a company that will demo instruments and speak): Biological AFM
The 2-day workshop includes all presentations, facility tours, student competitions, a vendor fair and plenty of tasty food during both days!
If you are interested in competing, please see the rules online. You can enter the completion when you register for the workshop online.
Workshop information, student presentation and poster competition rules, directions to MRL and registration are online at: www.mrl.illinois.edu/biostructures2014
Participant Registration: $60 ($20 of the fee will be refunded to students selected to present.)
Exhibitor Registration: $300
Hope we see some of you here! Lou Ann Miller
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230
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I have a question regarding to the disposal procedure for Osmium Tetroxide. I am new to TEM and have little knowledge of the staining agent OsO4. According the procedure I found online, I added twice amount of corn oil in the OsO4 bottle to neutralize it. However, after several hours, I observed the solution separated with the oil on the top while a black layer on the bottom. Should I stir the solution then? Since this is my first time dealing with the waste, I don't know what should I observe to know the neutralization process is done. Could someone explain more details of the procedure? I really appreciate your help!
Chya Yan
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Email: randy.ecidan-at-gmail.com Name: Randy
Title-Subject: [Filtered] SEM for Red Blood Cells
Message: Hi there,
I'm trying to image red blood cells in SEM. Are there good protocols that are simple for novice sample preparers? We are looking especially for things that work using easy to get reagents (no exotic, expensive, or highly toxic chemicals). We're having a lot of trouble, any suggestions will be greatly appreciated!
Thanks!
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From mohmedi-at-coltel.ru Sat Sep 13 10:13:23 2014 Return-Path: {mohmedi-at-coltel.ru} Received: from 178-78-43-77.broadband.coltel.ru ([178.78.43.77]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8DFDMoH016659 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 13 Sep 2014 10:13:22 -0500 Message-ID: {039469B5.6153211-at-coltel.ru}
Hi Michael, just burn your aperture plate by holding it in the blue part of a gas flame by means of forceps. Shape of the hole does'nt matter much. Success Peter
==============================Original Headers============================== 3, 25 -- From p.elbers90-at-upcmail.nl Sun Sep 14 15:59:51 2014 3, 25 -- Received: from fep16.mx.upcmail.net (fep16.mx.upcmail.net [62.179.121.36]) 3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8EKxnjL018913 3, 25 -- for {microscopy-at-microscopy.com} ; Sun, 14 Sep 2014 15:59:50 -0500 3, 25 -- Received: from edge02.upcmail.net ([192.168.13.237]) 3, 25 -- by viefep16-int.chello.at 3, 25 -- (InterMail vM.8.01.05.05 201-2260-151-110-20120111) with ESMTP 3, 25 -- id {20140914205948.ZYHS709.viefep16-int.chello.at-at-edge02.upcmail.net} 3, 25 -- for {microscopy-at-microscopy.com} ; Sun, 14 Sep 2014 22:59:48 +0200 3, 25 -- Received: from [192.168.1.10] ([89.98.195.194]) 3, 25 -- by edge02.upcmail.net with edge 3, 25 -- id r8zX1o00R4C7iPM018zXcB; Sun, 14 Sep 2014 22:59:31 +0200 3, 25 -- X-SourceIP: 89.98.195.194 3, 25 -- User-Agent: Microsoft-Entourage/12.36.0.130206 3, 25 -- Date: Sun, 14 Sep 2014 23:01:03 +0200 3, 25 -- Subject: TEM cleaning apertures 3, 25 -- From: Peter Elbers {p.elbers90-at-upcmail.nl} 3, 25 -- To: {Microscopy-at-microscopy.com} 3, 25 -- Message-ID: {D03BCEAF.45B8%p.elbers90-at-upcmail.nl} 3, 25 -- Thread-Topic: TEM cleaning apertures 3, 25 -- Thread-Index: Ac/QXv5cSu0feuQdd0GVgauwfJlEew== 3, 25 -- Mime-version: 1.0 3, 25 -- Content-type: text/plain; 3, 25 -- charset="US-ASCII" 3, 25 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
If the aperture is Molybdenum, then as alternative to burning you can soak it overnight in warm (30C to 40C, candle warmer works perfectly here) solution of 80% Micro-90 in 20% DI water, followed by 20min ultrasonic cleaning next morning in the solution of 5% Micro-90 95% DI water, followed by dipping in 99.9% IPA "low residue analytical grade for chromatography," followed by blow-dry by CDA, or DN2, or clean-room-approved duster. This procedure also works for anodes and beam-limiting apertures of SEM, if they are made of Mo.
Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
On 9/14/2014 5:01 PM, p.elbers90-at-upcmail.nl wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Michael, just burn your aperture plate by holding it in the blue part of } a gas flame by means of forceps. Shape of the hole does'nt matter much. } Success Peter } } } } ==============================Original Headers============================== } 3, 25 -- From p.elbers90-at-upcmail.nl Sun Sep 14 15:59:51 2014 } 3, 25 -- Received: from fep16.mx.upcmail.net (fep16.mx.upcmail.net [62.179.121.36]) } 3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8EKxnjL018913 } 3, 25 -- for {microscopy-at-microscopy.com} ; Sun, 14 Sep 2014 15:59:50 -0500 } 3, 25 -- Received: from edge02.upcmail.net ([192.168.13.237]) } 3, 25 -- by viefep16-int.chello.at } 3, 25 -- (InterMail vM.8.01.05.05 201-2260-151-110-20120111) with ESMTP } 3, 25 -- id {20140914205948.ZYHS709.viefep16-int.chello.at-at-edge02.upcmail.net} } 3, 25 -- for {microscopy-at-microscopy.com} ; Sun, 14 Sep 2014 22:59:48 +0200 } 3, 25 -- Received: from [192.168.1.10] ([89.98.195.194]) } 3, 25 -- by edge02.upcmail.net with edge } 3, 25 -- id r8zX1o00R4C7iPM018zXcB; Sun, 14 Sep 2014 22:59:31 +0200 } 3, 25 -- X-SourceIP: 89.98.195.194 } 3, 25 -- User-Agent: Microsoft-Entourage/12.36.0.130206 } 3, 25 -- Date: Sun, 14 Sep 2014 23:01:03 +0200 } 3, 25 -- Subject: TEM cleaning apertures } 3, 25 -- From: Peter Elbers {p.elbers90-at-upcmail.nl} } 3, 25 -- To: {Microscopy-at-microscopy.com} } 3, 25 -- Message-ID: {D03BCEAF.45B8%p.elbers90-at-upcmail.nl} } 3, 25 -- Thread-Topic: TEM cleaning apertures } 3, 25 -- Thread-Index: Ac/QXv5cSu0feuQdd0GVgauwfJlEew== } 3, 25 -- Mime-version: 1.0 } 3, 25 -- Content-type: text/plain; } 3, 25 -- charset="US-ASCII" } 3, 25 -- Content-transfer-encoding: 7bit } ==============================End of - Headers============================== }
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Email: hadeelzh-at-yahoo.com Name: hadeel
Title-Subject: [Filtered] Asphalt under sem
Message: hi ,can i study Asphalt under the scanning electron microscope in high vacuum mode and how they are being studied
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Sep 15 08:01:42 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8FD1ggP005597 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Sep 2014 08:01:42 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id 26A2C758E54 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Sep 2014 08:01:42 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id FqEb8a9-Jse9 for {microscopy-at-microscopy.com} ; 12, 28 -- Mon, 15 Sep 2014 08:01:33 -0500 (CDT) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id B0553758E41 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Sep 2014 08:01:33 -0500 (CDT) 12, 28 -- Message-ID: {5416E32D.4020901-at-microscopy.com} 12, 28 -- Date: Mon, 15 Sep 2014 08:01:33 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Asphalt under sem 12, 28 -- References: {201409150734.s8F7YS5h031938-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201409150734.s8F7YS5h031938-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201409150734.s8F7YS5h031938-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
It seems a little strange that you would ask the microscopy community how asphalt is being studied. I assume that you would have a question that you would like to answer rather than asking about others questions and techniques.
Thin layers of asphalt on aggregate might be studied at high vacuum. You would need to take normal precautions for dealing with insulating samples, i.e., low voltage and/or a metal coating.
You might also be prepared for some contamination in the chamber. You might volatilize some organic components that would condense within your chamber leading to a need for more frequent cleaning.
We tried looking at a polished section of asphalt in a high vacuum SEM years ago. We had problems getting to operational vacuum. Between volatile components and porosity, the sample would not pump down in the allowed time. We ended up buying an SEM with variable pressure capability. We did not have to pump to such a high level so we could reach operational vacuum quicker. VP mode meant we did not have to contend with charging. Also, we were in a vacuum mode where the residual gas helped sweep away the components that came off the sample. We did not have to worry about them depositing in the chamber.
I recommend that you locate a VP-SEM if you plan to do much asphalt or concrete work.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Monday, September 15, 2014 8:03 AM To: Straszheim, Warren E [BIOTC]
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Title-Subject: [Filtered] Asphalt under sem
Message: hi ,can i study Asphalt under the scanning electron microscope in high vacuum mode and how they are being studied
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From meds-discounted12-at-csb-bearing.com Mon Sep 15 12:40:42 2014 Return-Path: {meds-discounted12-at-csb-bearing.com} Received: from mail4.csb-bearing.com (localhost [113.165.22.106] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8FHecX4015950 for {microscopylistserverarchive2-at-microscopy.com} ; Mon, 15 Sep 2014 12:40:40 -0500 Message-Id: {201409151740.s8FHecX4015950-at-ns.microscopy.com}
X-from: 13qw9-at-queensu.ca ()
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Email: 13qw9-at-queensu.ca Name: Qiang Wang
Organization: Queen's University
Title-Subject: [Filtered] how to choose between different Wehnelt Apertures?
Message: Hi there, I am changing filament for Phillip CM20 TEM, and just found we have several different Wehnelt Apertures with various hole sizes in stock. So, what's the difference for different sizes of the apertures? How to choose one?
Cheers,
Jason
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Sep 15 20:41:55 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8G1ftEd023851 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Sep 2014 20:41:55 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 2999A759470 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Sep 2014 20:41:55 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id SylmNtwdrknB for {microscopy-at-microscopy.com} ; 16, 28 -- Mon, 15 Sep 2014 20:41:50 -0500 (CDT) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id C27E3759463 16, 28 -- for {microscopy-at-microscopy.com} ; Mon, 15 Sep 2014 20:41:50 -0500 (CDT) 16, 28 -- Message-ID: {5417955E.5070808-at-microscopy.com} 16, 28 -- Date: Mon, 15 Sep 2014 20:41:50 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW: how to choose between different Wehnelt Apertures? 16, 28 -- References: {201409152348.s8FNm9Vf021685-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201409152348.s8FNm9Vf021685-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201409152348.s8FNm9Vf021685-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello Jason, For Philips CM series following Wehnelt diaphragm are recommended.
Standard Tungsten Filament: 0.5 mm LaB6 Filament: 0.3 mm
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
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==============================Original Headers============================== 7, 39 -- From benada-at-biomed.cas.cz Tue Sep 16 01:40:20 2014 7, 39 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8G6eJiG012987 7, 39 -- for {microscopy-at-microscopy.com} ; Tue, 16 Sep 2014 01:40:19 -0500 7, 39 -- X-ASG-Debug-ID: 1410849618-05011e57af28a550001-4CH8be 7, 39 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id 1a4NIDmg0N01WVni (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Tue, 16 Sep 2014 08:40:18 +0200 (CEST) 7, 39 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 7, 39 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 7, 39 -- Received: from u117fs (c111mj.mbu.cas.cz [147.231.44.13]) 7, 39 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 7, 39 -- (No client certificate requested) 7, 39 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 6D6162C80E1 7, 39 -- for {microscopy-at-microscopy.com} ; Tue, 16 Sep 2014 08:40:18 +0200 (CEST) 7, 39 -- Date: Tue, 16 Sep 2014 08:40:16 +0200 7, 39 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 7, 39 -- To: {microscopy-at-microscopy.com} 7, 39 -- Subject: Fw: [Microscopy] viaWWW: how to choose between different Wehnelt 7, 39 -- Apertures? 7, 39 -- Message-ID: {20140916084016.5469e2b9-at-u117fs} 7, 39 -- X-ASG-Orig-Subj: Fw: [Microscopy] viaWWW: how to choose between different Wehnelt 7, 39 -- Apertures? 7, 39 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 7, 39 -- =?UTF-8?B?xIxS?= 7, 39 -- X-Mailer: Claws Mail 3.10.1 (GTK+ 2.24.10; i486-pc-linux-gnu) 7, 39 -- MIME-Version: 1.0 7, 39 -- Content-Type: text/plain; charset=US-ASCII 7, 39 -- Content-Transfer-Encoding: 7bit 7, 39 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 7, 39 -- X-Barracuda-Start-Time: 1410849618 7, 39 -- X-Barracuda-Encrypted: AES256-SHA 7, 39 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 7, 39 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 7, 39 -- X-Barracuda-BRTS-Status: 1 7, 39 -- X-Barracuda-Spam-Score: 0.00 7, 39 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 7, 39 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.9538 7, 39 -- Rule breakdown below 7, 39 -- pts rule name description 7, 39 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
McCrone Associates, Inc., a Chicago-area analytical and research laboratory, is looking to expand our staff by seeking several highly motivated and curious research scientists to solve materials and contamination problems using modern microscopy and microanalysis for our clients in a variety of industries. These positions offer a significant amount of autonomy and require the skills to handle multiple projects at any given time.
If you are an experienced microanalyst and looking for an interesting career in materials analysis, in a modern and innovative laboratory, please browse our current job opportunities at www.mccrone.com/careers.
You may apply directly through the website or by submitting a resume and cover letter to Gina Schuster, Director of Human Resources at gschuster-at-mccrone.com.
Elaine F. Schumacher | Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive . Westmont, IL 60559 P. 630.887.7100 | F. 630.887.7417 | www.mccrone.com
==============================Original Headers============================== 7, 42 -- From eschumacher-at-mccrone.com Tue Sep 16 08:00:34 2014 7, 42 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 7, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8GD0YGd019889 7, 42 -- for {microscopy-at-microscopy.com} ; Tue, 16 Sep 2014 08:00:34 -0500 7, 42 -- X-ASG-Debug-ID: 1410872433-07bbd4604817f2f0001-4CH8be 7, 42 -- Received: from TMGEX1.tmg.mccrone.com ([192.168.101.73]) by spam.mccrone.com with ESMTP id hfpYUGh7xZTNc0rF for {microscopy-at-microscopy.com} ; Tue, 16 Sep 2014 08:00:34 -0500 (CDT) 7, 42 -- X-Barracuda-Envelope-From: eschumacher-at-mccrone.com 7, 42 -- Received: from TMGEX2.tmg.mccrone.com (192.168.101.74) by 7, 42 -- TMGEX1.tmg.mccrone.com (192.168.101.73) with Microsoft SMTP Server (TLS) id 7, 42 -- 15.0.913.22; Tue, 16 Sep 2014 08:00:33 -0500 7, 42 -- Received: from TMGEX2.tmg.mccrone.com ([fe80::d433:2857:50d8:909e]) by 7, 42 -- TMGEX2.tmg.mccrone.com ([fe80::2c91:47d:9b63:8ba3%16]) with mapi id 7, 42 -- 15.00.0913.011; Tue, 16 Sep 2014 08:00:33 -0500 7, 42 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 7, 42 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 7, 42 -- Subject: Job Opportunities - Materials Microanalysis 7, 42 -- Thread-Topic: Job Opportunities - Materials Microanalysis 7, 42 -- X-ASG-Orig-Subj: Job Opportunities - Materials Microanalysis 7, 42 -- Thread-Index: Ac/RrX+MMvLLBHtJSxW2Bldi8uTKdA== 7, 42 -- Date: Tue, 16 Sep 2014 13:00:33 +0000 7, 42 -- Message-ID: {bd66ca698dc647a0b9dffaa067be4922-at-TMGEX2.tmg.mccrone.com} 7, 42 -- Accept-Language: en-US 7, 42 -- Content-Language: en-US 7, 42 -- X-MS-Has-Attach: 7, 42 -- X-MS-TNEF-Correlator: 7, 42 -- x-vipre-scanned: 055E14D300818C055E1620 7, 42 -- x-originating-ip: [192.168.101.84] 7, 42 -- Content-Type: text/plain; charset="iso-8859-1" 7, 42 -- MIME-Version: 1.0 7, 42 -- X-Barracuda-Connect: UNKNOWN[192.168.101.73] 7, 42 -- X-Barracuda-Start-Time: 1410872434 7, 42 -- X-Barracuda-URL: http://spam.mccrone.com:80/cgi-mod/mark.cgi 7, 42 -- X-Virus-Scanned: by bsmtpd at mccrone.com 7, 42 -- X-Barracuda-BRTS-Status: 1 7, 42 -- X-Barracuda-Spam-Score: 0.00 7, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 7, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.9546 7, 42 -- Rule breakdown below 7, 42 -- pts rule name description 7, 42 -- ---- ---------------------- -------------------------------------------------- 7, 42 -- Content-Transfer-Encoding: 8bit 7, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s8GD0YGd019889 ==============================End of - Headers==============================
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Email: susan.vanhorn-at-stonybrook.edu Name: Susan C. Van Horn
Organization: Stony Brook Univ
Title-Subject: [Filtered] tissue fixed in glutaraldehyde
Message: Has anyone experienced difficulty in sectioning (thick or thin) tissue that has been fixed in glutaraldehyde only and let sit in fix for days, weeks or even months??? embedding or sectioning problems?? thanks Sue
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Sep 16 20:11:32 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8H1BVSP004406 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 16 Sep 2014 20:11:31 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id C95B6759F35 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 16 Sep 2014 20:11:31 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id 2JBV-rXCDoky for {microscopy-at-microscopy.com} ; 13, 28 -- Tue, 16 Sep 2014 20:11:27 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 9F6EC759F28 13, 28 -- for {microscopy-at-microscopy.com} ; Tue, 16 Sep 2014 20:11:27 -0500 (CDT) 13, 28 -- Message-ID: {5418DFBF.3020309-at-microscopy.com} 13, 28 -- Date: Tue, 16 Sep 2014 20:11:27 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:tissue fixed in glutaraldehyde 13, 28 -- References: {201409162036.s8GKaRGa014468-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201409162036.s8GKaRGa014468-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201409162036.s8GKaRGa014468-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From botgibya-at-smart.az Wed Sep 17 03:25:11 2014 Return-Path: {botgibya-at-smart.az} Received: from host-95-86-129-50.smart.az (host-95-86-129-50.smart.az [95.86.129.50] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8H8PAAn027194 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 17 Sep 2014 03:25:10 -0500 Message-ID: {8B8E6554.0586893-at-smart.az}
Listers, Thanks everyone who responded to my request, I appreciate the help.
Gary
Gary M. Easton Scanners Corporation 90 Aileron Court, Ste. 6 Westminster, Maryland USA 21157 410.857.7633 (Office) 443-524-9631(Fax) 717.634.2226 (Mobile)
==============================Original Headers============================== 8, 19 -- From garyeaston-at-scannerscorp.com Wed Sep 17 09:16:57 2014 8, 19 -- Received: from sender1.zohomail.com (sender1.zohomail.com [74.201.84.155]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8HEGubP002489 8, 19 -- for {microscopy-at-microscopy.com} ; Wed, 17 Sep 2014 09:16:56 -0500 8, 19 -- Received: from [192.168.1.101] (69.244.87.213 [69.244.87.213]) by mx.zohomail.com 8, 19 -- with SMTPS id 1410963413346602.7090222026454; Wed, 17 Sep 2014 07:16:53 -0700 (PDT) 8, 19 -- From: "Gary Easton" {garyeaston-at-scannerscorp.com} 8, 19 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 19 -- Subject: Water Chiller for JEOL 840A 8, 19 -- Date: Wed, 17 Sep 2014 14:16:26 +0000 8, 19 -- Message-Id: {em0646862d-763a-4089-8b07-07ee118067f7-at-garypc} 8, 19 -- Reply-To: "Gary Easton" {garyeaston-at-scannerscorp.com} 8, 19 -- User-Agent: eM_Client/6.0.20648.0 8, 19 -- Mime-Version: 1.0 8, 19 -- Content-Type: text/plain; format=flowed; charset=utf-8 8, 19 -- X-ZohoMailClient: External 8, 19 -- X-Zoho-Virus-Status: 2 8, 19 -- Content-Transfer-Encoding: 8bit 8, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s8HEGubP002489 ==============================End of - Headers==============================
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realname - Emilie Colton Email - e.colton-at-utoronto.ca ORGANIZATION - University of Toronto EDUCATION - Graduate College LOCATION - Mississauga, Ontario, Canada SUBJECT_OF_QUESTION - Emerging Market Opportunities and Existing Limitations of Super Resolution Light Microscopy QUESTION - Hello,
I am a graduate student at the University of Toronto, and I am conducting research on the emerging market opportunities and the existing limitations surrounding super resolution light microscopy. As per our instructions for this project, we are required to inquire expert's opinions and insight on the matter. We would greatly appreciate any information/input so as to develop a detailed understanding of the subject.
Thank you in advance, Emilie Colton
==============================Original Headers============================== 5, 31 -- From oshel1pe-at-cmich.edu Wed Sep 17 15:23:21 2014 5, 31 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8HKNL0S028191 5, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Sep 2014 15:23:21 -0500 5, 31 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 5, 31 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s8HKNL0S025903 5, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Sep 2014 16:23:22 -0400 5, 31 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 5, 31 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 31 -- (TLS) id 14.3.181.6; Wed, 17 Sep 2014 16:23:20 -0400 5, 31 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 5, 31 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 5, 31 -- 14.3.181.6; Wed, 17 Sep 2014 16:23:19 -0400 5, 31 -- Message-ID: {5419EDB7.3090107-at-cmich.edu} 5, 31 -- Date: Wed, 17 Sep 2014 16:23:19 -0400 5, 31 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 5, 31 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 31 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 31 -- MIME-Version: 1.0 5, 31 -- To: micro {Microscopy-at-microscopy.com} 5, 31 -- Subject: Ask-A-Microscopist: Emerging Market Opportunities and Existing Limitations 5, 31 -- of Super Resolution Light Microscopy 5, 31 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 31 -- Content-Transfer-Encoding: 7bit 5, 31 -- X-Originating-IP: [141.209.160.122] 5, 31 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 31 -- X-Spam-Score: 0.20 () [Hold at 6.00] RDNS_NONE,_L_IAA,SPF(softfail:0),Bayes(0.0001:-0.5) 5, 31 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 31 -- X-CanItPRO-Stream: default 5, 31 -- X-Canit-Stats-ID: 05MQknl1g - 211e20755c78 - 20140917 5, 31 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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Email: kkusser-at-vgtifl.org Name: Kim
Organization: VGTI-FL
Title-Subject: [Filtered] Hypoxia setup on an inverted microscope
Message: Hi all,
We have a researcher who would like to do long term imaging of cardiomyocyt= es under hypoxic conditions.
Our microscope is currently setup with a CO2 chamber. (DV Elite on a Olympu= s inverted stand).
My idea is that we could add nitrogen (via a gas cylinder)... throw a flow = meter on it ( similar to the one we have for the CO2). ....run the N2 line = to the CO2 enclosure. Then, we would have to get some type of O2 sensor to = dial in the correct mix of N2 and CO2 for the target %O2.
We would like to do this as cheaply as possible, and I was wondering if any= one out there has any experience they would like to impart as to the feasib= ility of my semi-formed idea.
Thanks
Kim
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From niepuli-at-jardine-motors.com Thu Sep 18 08:36:57 2014 Return-Path: {niepuli-at-jardine-motors.com} Received: from mail3.jardine-motors.com ([95.86.89.62]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8IDasrb018097 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 18 Sep 2014 08:36:56 -0500 Message-ID: {5E040084.3389793-at-jardine-motors.com}
Dear Emilie,
I would be happy to discuss this project with you. Please contact me off-line at the phone number/email below.
Also, as an invited contributor, I wrote an overview article for BioPhotonics several years ago. Here is the link: http://microscopyeducation.com/images/Superresolution_BioPh_01_2011.pdf
Best regards, Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through Jan 2015. Call us today for a free training evaluation.
At 03:48 PM 9/17/2014, oshel1pe-at-cmich.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 13, 30 -- From bfoster-at-the-mip.com Thu Sep 18 11:06:26 2014 13, 30 -- Received: from barcelona.directrouter.com (barcelona.directrouter.com [72.249.107.2]) 13, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8IG6PbB022189 13, 30 -- for {Microscopy-at-Microscopy.com} ; Thu, 18 Sep 2014 11:06:26 -0500 13, 30 -- Message-Id: {201409181606.s8IG6PbB022189-at-ns.microscopy.com} 13, 30 -- Received: from 99-168-104-53.lightspeed.rcsntx.sbcglobal.net ([99.168.104.53]:59530 helo=Bs-PC.the-mip.com) 13, 30 -- by barcelona.directrouter.com with esmtpsa (TLSv1:DHE-RSA-AES256-SHA:256) 13, 30 -- (Exim 4.82) 13, 30 -- (envelope-from {bfoster-at-the-mip.com} ) 13, 30 -- id 1XUeDw-0001xP-M6; Thu, 18 Sep 2014 11:06:24 -0500 13, 30 -- Disposition-Notification-To: {bfoster-at-the-mip.com} 13, 30 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 30 -- Date: Thu, 18 Sep 2014 11:06:19 -0500 13, 30 -- To: oshel1pe-at-cmich.edu, e.colton-at-utoronto.ca, Microscopy-at-Microscopy.com 13, 30 -- From: Barbara Foster {bfoster-at-the-mip.com} 13, 30 -- Subject: Re: [Microscopy] Ask-A-Microscopist: Emerging Market 13, 30 -- Opportunities and Existing Limitations 13, 30 -- In-Reply-To: {201409172033.s8HKXNUw007932-at-ns.microscopy.com} 13, 30 -- References: {201409172033.s8HKXNUw007932-at-ns.microscopy.com} 13, 30 -- Mime-Version: 1.0 13, 30 -- Content-Type: text/plain; charset="us-ascii" 13, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 13, 30 -- X-AntiAbuse: Primary Hostname - barcelona.directrouter.com 13, 30 -- X-AntiAbuse: Original Domain - microscopy.com 13, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 13, 30 -- X-AntiAbuse: Sender Address Domain - the-mip.com 13, 30 -- X-Get-Message-Sender-Via: barcelona.directrouter.com: authenticated_id: bfoster-at-the-mip.com 13, 30 -- X-Source: 13, 30 -- X-Source-Args: 13, 30 -- X-Source-Dir: ==============================End of - Headers==============================
The high tension cable in our Jeol 5600 LV is not working and would like to replace it. Anybody having positive experience with a company that can do this with low cost, in the USA or in Europe? Thanks! yorgos
Servicing microscope for many years in Europe, I would talk to organisations that service hospital equipment like x-ray machines. In those days it was possible for these people to remake the cable if I provided the connections.
Good luck
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr] Sent: 19 September 2014 12:43 To: protrain-at-emcourses.com
Hello Steve What about the high vacuum seal on the cap of the microscope. Does this present a problem for the replacement of the cable? With many thanks yorgos
----- Original Message ----- X-from: {protrain-at-emcourses.com} To: {eikonika-at-otenet.gr} Sent: Friday, September 19, 2014 3:02 PM
X-from: robernal-at-u.northwestern.edu ()
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Title-Subject: [Filtered] Grounding Electronics inside TEM
Message: I have a holder for electrical contacting which I am using to run an experiment in-situ TEM. Parts of the circuit do go into the tip of the holder.
The circuit itself works pretty well in the TEM holder, whenever it is not inserted in the microscope. However, when the holder is inserted, noise becomes really high. I have discovered that I can connect the ground of my circuit to the microscope column (a screw or exposed metal in the goniometer), and it reduces the noise back to the usual level. However, this action activates the stage touch alarm, which locks the stage in place preventing any movement.
Does anybody have any details how the grounding in the TEM is done? Is the microscope exposed metal connected to earth ground? Is there any point that one may use in the TEM for grounding without affecting the stage?. Where does the TEM contacts the body of the holder electrically?
Thanks!!!
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Sep 19 09:34:49 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8JEYnmZ020279 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 19 Sep 2014 09:34:49 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 7773775C0AF 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 19 Sep 2014 09:34:49 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id xw6h-P4nXtuy for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 19 Sep 2014 09:34:44 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 5153275C09D 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 19 Sep 2014 09:34:44 -0500 (CDT) 14, 28 -- Message-ID: {541C3F04.1090705-at-microscopy.com} 14, 28 -- Date: Fri, 19 Sep 2014 09:34:44 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Grounding Electronics inside TEM 14, 28 -- References: {201409190049.s8J0nh8B018952-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201409190049.s8J0nh8B018952-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201409190049.s8J0nh8B018952-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From pharmacy-express1-at-seconduse.com Fri Sep 19 10:40:24 2014 Return-Path: {pharmacy-express1-at-seconduse.com} Received: from smtp2.seconduse.com ([93.170.157.114]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8JFeM32007950 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 19 Sep 2014 10:40:22 -0500 Message-Id: {201409191540.s8JFeM32007950-at-ns.microscopy.com}
I know the topic of service providers for electron microscopes comes up fai= rly regularly, but what about for optical microscopes? Does anyone have exp= erience or informed opinions about working with a third-party distributor o= r service provider vs the manufacturer's service center?
We have a couple of Zeiss AxioImagers and an AxioZoom, and most of the time= they just work. Once in a while a motorized component goes out. We have be= en working with a distributor for service, but I wonder if there are benefi= ts to outweigh the higher cost of working with the Zeiss service center.
None of us here have a lot of experience working on optical microscopes oth= er than sitting in front on one to look at stuff, and the service guy isn't= real strong on training. Are there some recommended guides for alignments = or imaging conditions to use? We are looking at materials (thin films of me= tals or polymers, silicon, particles and defects).
Thanks to everyone on this list who makes it such a valuable resource.
Jonathan Abbott
==============================Original Headers============================== 11, 23 -- From jabbott-at-moxtek.com Fri Sep 19 11:43:57 2014 11, 23 -- Received: from hub031-co-2.exch031.serverdata.net (hub031-co-2.exch031.serverdata.net [199.193.204.136]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8JGhv6Z028166 11, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Sep 2014 11:43:57 -0500 11, 23 -- Received: from MBX031-W1-CO-8.exch031.domain.local ([10.224.113.89]) by 11, 23 -- HUB031-CO-2.exch031.domain.local ([10.224.113.43]) with mapi id 11, 23 -- 14.03.0174.001; Fri, 19 Sep 2014 09:43:55 -0700 11, 23 -- From: Jonathan Abbott {jabbott-at-moxtek.com} 11, 23 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 23 -- Subject: Optical microscope service 11, 23 -- Thread-Topic: Optical microscope service 11, 23 -- Thread-Index: Ac/UKOZtjOXa/7f8R6acz59pbRYQDQ== 11, 23 -- Date: Fri, 19 Sep 2014 16:43:54 +0000 11, 23 -- Message-ID: {23ACF4C758E77B4293F16E8F1E61F983445E13F4-at-mbx031-w1-co-8.exch031.domain.local} 11, 23 -- Accept-Language: en-US 11, 23 -- Content-Language: en-US 11, 23 -- X-MS-Has-Attach: 11, 23 -- X-MS-TNEF-Correlator: 11, 23 -- x-originating-ip: [107.0.83.138] 11, 23 -- Content-Type: text/plain; charset="us-ascii" 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s8JGhv6Z028166 ==============================End of - Headers==============================
From myzedqa-at-comcastbusiness.net Fri Sep 19 13:39:54 2014 Return-Path: {myzedqa-at-comcastbusiness.net} Received: from 50-249-232-57-static.hfc.comcastbusiness.net ([168.93.85.42]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8JIdsVI017213 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 19 Sep 2014 13:39:54 -0500 Message-ID: {AABA346D.2220273-at-comcastbusiness.net}
The deadline for the student competition is approaching!
Win FAME.... GLORY..... $100 Cash Prize!
At our 3rd annual biological conference, we will be having 2 student competitions for those working with biological substances, or a mix of biological and materials.
One competition is for oral presentation, one for poster presentations. You do not have to give a talk to enter a poster, but if you talk, we would like you to also present a poster.
How to enter:
1. Ask your advisor what credit card ( or account if in Illinois university system) to register on ( $60 for whole meeting, $20 refund if you present a talk) -- Price includes 2 days of food.
2. Write a 1/2 page abstract and gather any images you wish to submit.
3. Go to this web site, and look at the blue box menu to the right, Choose payment type and begin the registration, there will be a button for uploading files.
==============================Original Headers============================== 36, 37 -- From lamiller-at-illinois.edu Tue Sep 23 08:05:54 2014 36, 37 -- Received: from pps04.cites.illinois.edu (pps04.cites.illinois.edu [192.17.82.101]) 36, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8ND5sN0023794 36, 37 -- for {Microscopy-at-Microscopy.com} ; Tue, 23 Sep 2014 08:05:54 -0500 36, 37 -- Received: from citesht3.cites.illinois.edu (citesht3.cites.illinois.edu [128.174.34.208]) 36, 37 -- by pps04.cites.illinois.edu (8.14.5/8.14.5) with ESMTP id s8ND5gXt011398 36, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 36, 37 -- for {Microscopy-at-Microscopy.com} ; Tue, 23 Sep 2014 08:05:54 -0500 36, 37 -- Received: from CHIMBX2.ad.uillinois.edu ([169.254.7.167]) by 36, 37 -- CITESHT3.ad.uillinois.edu ([128.174.34.208]) with mapi id 14.03.0195.001; 36, 37 -- Tue, 23 Sep 2014 08:05:39 -0500 36, 37 -- From: "Miller, Lou A" {lamiller-at-illinois.edu} 36, 37 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 36, 37 -- Subject: Microscopy & analysis competition! Win Fame, Glory, Cash! at the 36, 37 -- University of Illinois. 36, 37 -- Thread-Topic: Microscopy & analysis competition! Win Fame, Glory, Cash! at 36, 37 -- the University of Illinois. 36, 37 -- Thread-Index: AQHP1y8Sfy8t+1WrYEeQCvfsuHnzmw== 36, 37 -- Date: Tue, 23 Sep 2014 13:05:38 +0000 36, 37 -- Message-ID: {A241F645-D03E-4678-B374-422D082FC97B-at-illinois.edu} 36, 37 -- Accept-Language: en-US 36, 37 -- Content-Language: en-US 36, 37 -- X-MS-Has-Attach: 36, 37 -- X-MS-TNEF-Correlator: 36, 37 -- x-originating-ip: [128.174.34.199] 36, 37 -- Content-Type: text/plain; charset="us-ascii" 36, 37 -- Content-ID: {AEEF634AADD52E4BA635812C783AE286-at-mx.uillinois.edu} 36, 37 -- MIME-Version: 1.0 36, 37 -- X-Spam-Score: 0 36, 37 -- X-Spam-Details: rule=cautious_plus_nq_notspam policy=cautious_plus_nq score=0 spamscore=0 36, 37 -- suspectscore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 36, 37 -- adjust=0 reason=mlx scancount=1 engine=7.0.1-1402240000 36, 37 -- definitions=main-1409230123 36, 37 -- X-Spam-OrigSender: lamiller-at-illinois.edu 36, 37 -- X-Spam-Bar: 36, 37 -- Content-Transfer-Encoding: 8bit 36, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s8ND5sN0023794 ==============================End of - Headers==============================
From kriuyyi-at-isaaceld.k12.az.us Tue Sep 23 13:17:34 2014 Return-Path: {kriuyyi-at-isaaceld.k12.az.us} Received: from mail4.isaaceld.k12.az.us ([94.72.63.18]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8NIHX4n027593 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 23 Sep 2014 13:17:34 -0500 Message-ID: {03F803F6.5889825-at-isaaceld.k12.az.us}
When I was at Intel in the SF Bay Area, we used two private optical shops with great satisfaction. They were:
Optotek 800-924-6023 http://www.optotek.net/
and
Serco 800-483-0508
John Mardinly, ASU
-----Original Message----- X-from: jabbott-at-moxtek.com [mailto:jabbott-at-moxtek.com] Sent: Friday, September 19, 2014 9:53 AM To: John Mardinly
I know the topic of service providers for electron microscopes comes up fai= rly regularly, but what about for optical microscopes? Does anyone have exp= erience or informed opinions about working with a third-party distributor o= r service provider vs the manufacturer's service center?
We have a couple of Zeiss AxioImagers and an AxioZoom, and most of the time= they just work. Once in a while a motorized component goes out. We have be= en working with a distributor for service, but I wonder if there are benefi= ts to outweigh the higher cost of working with the Zeiss service center.
None of us here have a lot of experience working on optical microscopes oth= er than sitting in front on one to look at stuff, and the service guy isn't= real strong on training. Are there some recommended guides for alignments = or imaging conditions to use? We are looking at materials (thin films of me= tals or polymers, silicon, particles and defects).
Thanks to everyone on this list who makes it such a valuable resource.
Jonathan Abbott
==============================Original Headers============================== 11, 23 -- From jabbott-at-moxtek.com Fri Sep 19 11:43:57 2014 11, 23 -- Received: from hub031-co-2.exch031.serverdata.net (hub031-co-2.exch031.serverdata.net [199.193.204.136]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8JGhv6Z028166 11, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Sep 2014 11:43:57 -0500 11, 23 -- Received: from MBX031-W1-CO-8.exch031.domain.local ([10.224.113.89]) by 11, 23 -- HUB031-CO-2.exch031.domain.local ([10.224.113.43]) with mapi id 11, 23 -- 14.03.0174.001; Fri, 19 Sep 2014 09:43:55 -0700 11, 23 -- From: Jonathan Abbott {jabbott-at-moxtek.com} 11, 23 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 23 -- Subject: Optical microscope service 11, 23 -- Thread-Topic: Optical microscope service 11, 23 -- Thread-Index: Ac/UKOZtjOXa/7f8R6acz59pbRYQDQ== 11, 23 -- Date: Fri, 19 Sep 2014 16:43:54 +0000 11, 23 -- Message-ID: {23ACF4C758E77B4293F16E8F1E61F983445E13F4-at-mbx031-w1-co-8.exch031.domain.local} 11, 23 -- Accept-Language: en-US 11, 23 -- Content-Language: en-US 11, 23 -- X-MS-Has-Attach: 11, 23 -- X-MS-TNEF-Correlator: 11, 23 -- x-originating-ip: [107.0.83.138] 11, 23 -- Content-Type: text/plain; charset="us-ascii" 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s8JGhv6Z028166 ==============================End of - Headers==============================
From medications-best2-at-triolan.net Tue Sep 23 15:08:12 2014 Return-Path: {medications-best2-at-triolan.net} Received: from 50.16.57.37.triolan.net (50.16.57.37.triolan.net [37.57.16.50] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8NK8A2L024932 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 23 Sep 2014 15:08:11 -0500 Message-Id: {201409232008.s8NK8A2L024932-at-ns.microscopy.com}
Hello,
for our small lab we are looking for a used sputter coater to coat SEM samples (Au, Pt or similar). In particular, we would like to coat older decapsulated IC (integrated circuit) models to get better results with voltage contrast imaging. Since we're on a very small budget, I was wondering if anyone on this list has an old sputter coater that is no longer in use and could be sold to us. Necessary repairs would be no problem as long as we can still get spare parts.
A few months ago our lab acquired an old JEOL IC-848A SEM. We found that the CRT is no longer working (the yoke coils are shorted out). The CRT is a Toshiba, Model #E2755B7, with a Totoku yoke, Model #EYS-03-0600.
We would like to replace this CRT. Online searches have shown one or two places which have them, but those that do are without a yoke. Does anyone know where to find a complete replacement CRT (or yoke)? Or, if that's not possible, what a compatible replacement would be? Any help would be appreciated.
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Email: mclymer-at-datacolor.com Name: Mark Clymer
Organization: Datacolor Inc.
Title-Subject: [Filtered] [LM] Webinar recording: "A Better Choice than Photoshop..."
Message: The webinar recording of "A Better Choice than Photoshop for Color Brightfield Microscope Images" is now available at http://tinyurl.com/puyj339. In this webinar, Jerry Sedgewick (Imaging and Analysis, LLC) demonstrates the common uses of Photoshop to adjust color in scientific images, and then demonstrates better, faster results using Datacolor ChromaCal.
CAVEAT: Datacolor is a manufacturer of color calibration solutions for microscopy.
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Email: rms-at-angstrom.us Name: Bob Sommerville
Organization: Angstrom Scientific Inc.
Title-Subject: [Filtered] Sales Representative Position Available, Mid Atlantic
Message: Position: Technical Sales Representative - Mid Atlantic US Territory: Dependent on the candidate but will include MD, DE, DC, VA and Eastern PA. Products: Sales and support of the following products/companies: Hitachi: Bench-top Scanning Electron Microscopes (SEM) DENSsolutions Heating and biasing holders for TEM Kleindiek: Nano-manipulators & Stages AppFive: Electron Diffraction (TEM) Jordan Valley: X-ray Diffraction (XRD) XEI: Plasma Cleaners Deben Microscope Accessories Microtrac Particle size measurement MelBuild TEM sample holders
Position Description: This is an exciting position selling and supporting Hitachi bench-top scanning electron microscopes which expand on the capabilities of optical microscopes. In addition, Angstrom Scientific Inc. represents a number of leading companies in the electron microscopy and related markets and sells select used scanning electron microscopes. The ideal candidate will preferably have a demonstrated track record of success ( 2-3 years) selling technical products into the academic, research and industrial markets. Optical microscopy or SEM/TEM and/or particle measurement related experience is a definite plus. Academic background should be at min an associateÂ’s degree in science (BS or masters preferred) The successful candidate will live in the above listed states. Angstrom Scientific Inc is a growing distributor of products targeted to the nanotechnology, research and semiconductor markets with exciting new and novel technology Angstrom Scientific Inc offers competitive salary and benefits, commission, car allowance and expenses. We are an equal opportunity employer. Interested candidates should submit their resume together with salary history to: Bob Sommerville, General Manager, Angstrom Scientific Inc. e-mail: rms-at-angstrom.us
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both heckman-at-bgsu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: heckman-at-bgsu.edu Name: Carol Heckman
Organization: Bowling Green State University
Title-Subject: [Filtered] Michigan Microscopy and Microanalysis Society meeting
Message: MMMS announces the 2104 meeting will be held Thursday November 6th
Place: McGregor Memorial Conference Center, Wayne State University Confirmed Speakers: C. Barry Carter, former President of Microscopy Society of America, on changes and challenges in the field of TEM
Michael Reedy, Duke University, on X-ray diffraction, electron microscopy and electron tomography
Further information is found at:
http://www.michmicroscopy.org
NOTE: Abstract deadline is October 21st Registration deadline is October 31st.
Carol Heckman, Secretary of MMMS
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jcderojas-at-ucdavis.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jcderojas-at-ucdavis.edu Name: Julius De Rojas
Our lab has recently acquired an old JEOL IC-848A that was out of commission for two years. We've finally got it up and started, but we found that we have a bad CRT. The yoke coils seems to be shorted out.
The CRT is a Toshiba, Model #E2755B7, with yoke made Totoku, Model #EYS-03-0600. Does anyone know where a replacement yoke can be purchased? Or, if not, what an equivalent replacement CRT would be?
Any assistance would be much appreciated.
Julius De Rojas
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=========================================== Dr. Nestor J. Zaluzec 797 Bonnie Brae Ct. Bolingbrook, Illinois 60440, USA Tel:1-630-739-1160 Alternate: 1-530-NES-TORZ (1-530-637-8679) has Voice Mail Email: Zaluzec-at-Microscopy.Com
From ruequsiu-at-garciastents.com Wed Sep 24 22:07:09 2014 Return-Path: {ruequsiu-at-garciastents.com} Received: from smtp4.garciastents.com ([145.255.0.54]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8P377kf009135 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 24 Sep 2014 22:07:08 -0500 X-AntiVirus: Checked by Dr.Web [version: 7.0.4.09220, engine: 7.0.100.09170, virus records: 5463507, updated: 25.09.2014] Message-ID: {54A3B4E6.6775802-at-garciastents.com}
I am not that familiar with Jeol specifically, but done fair share of upgrades and repairs.... does this monitor display regular RS-170 video signal (analog TV standard?), or does it monitor a proprietary feed?
If monitor works with standard TV signal then any LCD TV monitor could be plugged in instead; if signal is not standard then decent video input card (like Epiphan VGADVI for example, or something slightly more sophisticated in tough cases) could be used to digitize the signal and display it on PC monitor.
Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
On 9/24/2014 4:11 PM, jcderojas-at-ucdavis.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello All, } } A few months ago our lab acquired an old JEOL IC-848A SEM. We found } that the CRT is no longer working (the yoke coils are shorted out). } The CRT is a Toshiba, Model #E2755B7, with a Totoku yoke, Model } #EYS-03-0600. } } We would like to replace this CRT. Online searches have shown one or } two places which have them, but those that do are without a yoke. Does } anyone know where to find a complete replacement CRT (or yoke)? Or, if } that's not possible, what a compatible replacement would be? Any help } would be appreciated. } } Thanks you in advance, } } Julius De Rojas } } ==============================Original Headers============================== } 5, 28 -- From jcderojas-at-ucdavis.edu Wed Sep 24 15:09:25 2014 } 5, 28 -- Received: from mail-lb0-f182.google.com (mail-lb0-f182.google.com [209.85.217.182]) } 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8OK9OZA005492 } 5, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Sep 2014 15:09:25 -0500 } 5, 28 -- Received: by mail-lb0-f182.google.com with SMTP id u10so11540620lbd.27 } 5, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Sep 2014 13:09:24 -0700 (PDT) } 5, 28 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 28 -- d=1e100.net; s=20130820; } 5, 28 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to } 5, 28 -- :content-type; } 5, 28 -- bh=/TyrTuc5M9wJaN6dS6gRvqMwzJ3l8wd732PLBfSsn+0=; } 5, 28 -- b=hj2ciJIB38CvLYMHSIvLbyBXQ8xXDoCnrUhCg6+drUdJ+FnhB6+57nSdb7K7m5UpRB } 5, 28 -- XZva2Mr+yxYAJbjpl3mnLcoZddLlSrrHMwkDWD3GgZLrjFS4TxhBzMXOtGV89MA+OjNy } 5, 28 -- apvB1WK48382iVtLJbHOIXeqw5v/wpZcFNiDthXPJoByRg5voRGjHhSPwslQjICls3Qx } 5, 28 -- PRrC6OD/fQv6q+mU0rTkExR2qRoo2roZmrZHeZa7/+A74J0KZDgz5jHF/U2K+cG/bBae } 5, 28 -- t3AkLPdvS8Fxlr5wqRK05xUzfAee78GXurdA0RWwnQUfOAgGFqh+a0BSVOikqc/0+GKK } 5, 28 -- RLQQ== } 5, 28 -- X-Gm-Message-State: ALoCoQm+Ota0QtT2BSROIKf8M7TBsBUs88jzVEafB/vg/+9DD2uTxxzLup/d8mA3pbwt5sl3ybHW } 5, 28 -- MIME-Version: 1.0 } 5, 28 -- X-Received: by 10.112.128.135 with SMTP id no7mr7779557lbb.74.1411589363791; } 5, 28 -- Wed, 24 Sep 2014 13:09:23 -0700 (PDT) } 5, 28 -- Received: by 10.112.201.66 with HTTP; Wed, 24 Sep 2014 13:09:23 -0700 (PDT) } 5, 28 -- Date: Wed, 24 Sep 2014 13:09:23 -0700 } 5, 28 -- Message-ID: {CAFzinyxLYTbUwsNU9ke2x6AVwx3T7jH4M4p-Y8wJdUSNZwLbQw-at-mail.gmail.com} } 5, 28 -- Subject: SEM JEOL IC-848A CRT (or yoke) } 5, 28 -- From: Julius De Rojas {jcderojas-at-ucdavis.edu} } 5, 28 -- To: Microscopy-at-microscopy.com } 5, 28 -- Content-Type: text/plain; charset=UTF-8 } ==============================End of - Headers============================== }
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realname - Megha Dubey Email - meghandubey-at-gmail.com EDUCATION - Graduate College QUESTION - I am a PhD student, with thesis work completed, however with an uncertainty of getting a PhD degree. I did my work in high temperature alloy oxidation using different microscopic tools to obtain information from macroscopic to atomic scale. I would like to develop my career in field of microscopy. Are there any specialized course in USA which will allow me to get a degree in field of microscopy? Thank you for the help!
Regards, Megha
==============================Original Headers============================== 3, 30 -- From oshel1pe-at-cmich.edu Thu Sep 25 14:23:42 2014 3, 30 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8PJNgq6008018 3, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Sep 2014 14:23:42 -0500 3, 30 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 3, 30 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s8PJNXLP022363 3, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Sep 2014 15:23:45 -0400 3, 30 -- Received: from cas1.central.cmich.local (2002:8dd1:f8c::8dd1:f8c) by 3, 30 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 3, 30 -- (TLS) id 14.3.195.1; Thu, 25 Sep 2014 15:23:27 -0400 3, 30 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 3, 30 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 3, 30 -- 14.3.195.1; Thu, 25 Sep 2014 15:23:26 -0400 3, 30 -- Message-ID: {54246BAD.5000601-at-cmich.edu} 3, 30 -- Date: Thu, 25 Sep 2014 15:23:25 -0400 3, 30 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 3, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 30 -- MIME-Version: 1.0 3, 30 -- To: micro {Microscopy-at-microscopy.com} 3, 30 -- Subject: Ask-a-Microscopist: PhD level specialized materials courses? 3, 30 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 30 -- Content-Transfer-Encoding: 7bit 3, 30 -- X-Originating-IP: [141.209.160.122] 3, 30 -- X-Bayes-Prob: 0.0002 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 30 -- X-Spam-Score: -1.20 () [Hold at 6.00] RDNS_NONE,_L_IAA,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0002:-0.5) 3, 30 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 3, 30 -- X-CanItPRO-Stream: default 3, 30 -- X-Canit-Stats-ID: 05MTvnJI5 - f2993e86e4b8 - 20140925 3, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Northern California Society for Microscopy Fall Meeting
Message: The Northern California Society for Microscopy will hold its fall meeting on Thursday October 9 at JH Technologies in Fremont, CA.
Please join us for dinner and an excellent group of speakers. Additional information is on our website: www.ncsmicroscopy.org We hope to see you Oct 9!
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From cufqenvy-at-interaccess.com Fri Sep 26 06:46:17 2014 Return-Path: {cufqenvy-at-interaccess.com} Received: from smtp4.interaccess.com ([91.217.2.226]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8QBkGKD025228 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 26 Sep 2014 06:46:17 -0500 Message-ID: {F641B2A2.9735558-at-interaccess.com}
X-from: nxa157-at-case.edu () To: Zaluzec-at-MICROSCOPY.COM
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Case Western Reserve University
Title-Subject: [Filtered] MSNO 2014 Fall meeting
Message: On behalf of Microscopy Society of Northeastern Ohio (MSNO), I'd like to invite you to join MSNO Fall meeting. This meeting is on Tuesday, October 14 at 4:00-8:30 pm at Goodyear Auditorium, Goodyear Polymer Center, University of Akron.
Dr. Simon C. Watkins (University of Pittsburgh) will present "Novel Probes and microscopies to dissect molecular processing in cystic fibrosis".
Dr. Nita Sahai ( University of Akron) will present
The Potential Role of Minerals in the Origins of Life
You can register on line at http://www.msneo.org/meetings.html
Hope to see you in the Fall meeting.
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I'd like to announce the next meeting of the Mountain States Society of Electron Microscopists and Colorado Microbeam Analysis Society to be held Thursday October 9 from 11A - 4P. This meeting breaks from our traditional dinner meeting and allows for more talks and networking time and has something for everyone.
In addition to the meeting there is an automated mineralogy by SEM/EDS workshop held by Tescan at the Colorado School of Mines from 9A-11A. Please contact Colleen Leary (cleary-at-tescan-usa.com or 724-772-7433) for more details.
October 9, 2014 Table Mountain Inn, Golden CO
9:00-11:00A Automated Mineralogy Workshop by Jack Mershon, Tescan. Held at Colorado School of Mines. Contact Colleen Leary (cleary-at-tescan-usa.com or 724-772-7433) for details
11:00-11:15 Check In and Registration Table Mountain Inn
11:15-12:15 Lunch Buffet Chicken Tortilla Soup, Chimayó Chips & Salsa, Dijon Mustard Potato Salad OR Tangy Fresh Made Coleslaw, Assorted Wraps – Roasted Turkey, Ham and Roast Beef – Served with Grilled Portobello Mushrooms, Hummus & Assorted Olives, Freshly Baked Brownies & Cookies
12:30-1:15P Deborah Hall, Rush University Medical Center “Understanding the Effects of Wear Particles: Lessons Learned from Postmortem Retrievals”
1:15-1:45P Brian Gorman, Colorado School of Mines “Determining the Properties of Oxides using Static and Dynamic Atom Probe Tomography”
1:45-2:00P Vendor Talks, Gatan, EDAX, JEOL
2:00-2:30 Coffee Break
2:30-3:15P Ed Vicenzi, Museum Conservation Institute, Smithsonian Institution ”Examination of a 19th Century Daguerreotype Photograph using High Resolution Scanning Transmission Electron Microscopy for 2D and 3D Nanoscale Imaging and Analysis”
3:15-3:45P Jack Mershon, Tescan “Hyperspectral Imaging of Geological Thin Sections”
3:45-4:00P Adam Stokes, Colorado School of Mines “Atom Probe Tomography Study on Cu(In,Ga)Se2 Grain Boundaries and Ordered Defect Phase Transition”
4:00P Adjourn
$25/professional $5/student
Please register online by October 3rd so we have an accurate number for catering purposes. For online registration you can pay with a credit card or choose to send a check payable to MSSEM/CoMAS, c/o John Chandler, 2309 Cheyenne St, Golden CO 80401.
Registration link http://microbeamanalysis.org/topical-conferences/colorado-mas-june-2014/registration-cmas-june-2014-meeting
Announcement link http://comas.geoloweb.ch/events.php
Thank you to our sponsors to date: Tescan, EDAX, Gatan, Jeol, Brukr, Cameca, and Zeiss
From dzexifto-at-iinet.net.au Tue Sep 30 08:45:57 2014 Return-Path: {dzexifto-at-iinet.net.au} Received: from 106-69-53-148.dyn.iinet.net.au (106-69-53-148.dyn.iinet.net.au [106.69.53.148]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s8UDjtRp029751 for {microscopylistserverarchive2-at-microscopy.com} ; Tue, 30 Sep 2014 08:45:56 -0500 Message-ID: {E985F273.5924784-at-iinet.net.au}
Dear all
The Southern California Society for Microscopy and Microanalysis (SCSMM) announce their Fall Meeting.
The meeting will be held on Thursday, October 23rd from 5:30 pm at the Argyros Auditorium of Arnold and Mabel Beckman Center at the City of Hope, Duarte, California. A buffet dinner will be served.
Our fall meeting will be dedicated to the Correlative Light and Electron Microscopy. The featured speakers will be Thomas J. Deerinck (NCMIR and UCSD), and Grayson Chadwick (Caltech).
Please see full program on our website: http://www.scsmm.org/
In order to register, Please RSVP by email at micromark-at-juno.com. Respond no later than 5:00 p.m. Friday, October 17th.
The cost is $25 for professionals and $10 for students. Membership dues can be paid at the door on October 23rd.
Nonmembers are welcome to attend.
Please pass along to those you feel would be interested. You can also find us on the facebook
Look forward to seeing you at the meeting!
==============================Original Headers============================== 12, 30 -- From sergey-at-seas.ucla.edu Wed Oct 1 12:48:53 2014 12, 30 -- Received: from smtp-a.seas.ucla.edu (smtp-a.seas.ucla.edu [164.67.100.45]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s91Hmqdi032510 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 12:48:52 -0500 12, 30 -- Received: from localhost (localhost [127.0.0.1]) 12, 30 -- by smtp-a.seas.ucla.edu (8.14.3/8.14.3/MTA-TX) with ESMTP id s91HmENo008251 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 10:48:51 -0700 12, 30 -- X-Virus-Scanned: amavisd-new at seas.ucla.edu 12, 30 -- Received: from smtp-a.seas.ucla.edu ([127.0.0.1]) 12, 30 -- by localhost (smtp-a.seas.ucla.edu [127.0.0.1]) (amavisd-new, port 10024) 12, 30 -- with LMTP id yZGLAOQO50hj for {microscopy-at-microscopy.com} ; 12, 30 -- Wed, 1 Oct 2014 10:48:47 -0700 (PDT) 12, 30 -- Received: from webmail.seas.ucla.edu (mail01.seas.ucla.edu [164.67.100.157]) 12, 30 -- by smtp-a.seas.ucla.edu (8.14.3/8.14.3/MTA-RX) with ESMTP id s91Hmie5008350 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 10:48:44 -0700 12, 30 -- Received: from 128.97.83.27 12, 30 -- (SquirrelMail authenticated user sergey) 12, 30 -- by webmail.seas.ucla.edu with HTTP; 12, 30 -- Wed, 1 Oct 2014 10:48:44 -0700 (PDT) 12, 30 -- Message-ID: {50268.128.97.83.27.1412185724.squirrel-at-webmail.seas.ucla.edu} 12, 30 -- Date: Wed, 1 Oct 2014 10:48:44 -0700 (PDT) 12, 30 -- Subject: SCSMM fall meeting 12, 30 -- From: sergey-at-seas.ucla.edu 12, 30 -- To: microscopy-at-microscopy.com 12, 30 -- User-Agent: SquirrelMail/1.4.8-5.el5.centos.13 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Type: text/plain;charset=iso-8859-1 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-Priority: 3 (Normal) 12, 30 -- Importance: Normal ==============================End of - Headers==============================
I have a student completing our EM program who has asked for some advice.
She has experience doing technical writing and will soon have a certificate in biological EM.
She asked if there were any career possibilities that could combine EM knowledge and technical writing skills.
What do I tell her?
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 17, 38 -- From prvs=13444c0b4d=jkrupp-at-deltacollege.edu Wed Oct 1 14:51:59 2014 17, 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.237]) 17, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id s91JpwSB021360 17, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 14:51:58 -0500 17, 38 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 17, 38 -- by localhost (Email Security Appliance) with SMTP id F15A69D27A4_42C5B5BB 17, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 19:51:55 +0000 (GMT) 17, 38 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) 17, 38 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 8B2BE9D26C4_42C5B55F 17, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 19:51:49 +0000 (GMT) 17, 38 -- Received: from localhost (localhost [127.0.0.1]) 17, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 884D1F7FABD4 17, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 12:51:49 -0700 (PDT) 17, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 17, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 17, 38 -- with ESMTP id Y7zdegNcXq3J for {Microscopy-at-microscopy.com} ; 17, 38 -- Wed, 1 Oct 2014 12:51:45 -0700 (PDT) 17, 38 -- Received: from localhost (localhost [127.0.0.1]) 17, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id EEF76FB285F5 17, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 12:51:44 -0700 (PDT) 17, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 17, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 17, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 17, 38 -- with ESMTP id 0sFWUunlMmJV for {Microscopy-at-microscopy.com} ; 17, 38 -- Wed, 1 Oct 2014 12:51:44 -0700 (PDT) 17, 38 -- Received: from [10.146.0.13] (unknown [10.146.0.13]) 17, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id D32BCFB285F1 17, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 12:51:44 -0700 (PDT) 17, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 17, 38 -- Content-Type: text/plain; charset=us-ascii 17, 38 -- Subject: Technical writing 17, 38 -- Date: Wed, 1 Oct 2014 12:51:44 -0700 17, 38 -- Message-Id: {5D17345F-CB43-4284-8707-98474CDF1A3E-at-deltacollege.edu} 17, 38 -- To: Microscopy-at-microscopy.com 17, 38 -- Mime-Version: 1.0 (Apple Message framework v1085) 17, 38 -- X-Mailer: Apple Mail (2.1085) 17, 38 -- Content-Transfer-Encoding: 8bit 17, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s91JpwSB021360 ==============================End of - Headers==============================
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Email: caromill-at-iupui.edu Name: Caroline A Miller
Organization: MSORV
Title-Subject: [Filtered] Fall Meeting
Message: Microscopy Society of the Ohio River Valley Announces its 2014 Fall Meeting on October 23rd from 3-7:30 PM
This meeting will be held at Miami University, Oxford, OH, Center for Advanced Microscopy and Imaging, Room 9 Upham Hall
Featuring Student Competition for Best Microscopy Presentation
Students present your work and have a chance to win a MSORV Travel Award to the 2015 M&M meeting in Portland, OR for up to $1000.
Submission Deadline: Oct 6th, Send a 1 page abstract to Dave Tomlin, dtomlin-at-azimuth-corp.com
For more information check out the MSORV website.
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] error message
Message: Hi!!
The FEI Quanta 200 showing the message "filament blown off" on the contrary they are able to see the filament emission but the filament emission signal turns from green to red. The error occurs after some time when the HT and filament was switched on. It is almost 3-4 four months now they are getting such erratic problems. Not able to trace out the reason for this. Is it suitable to analyzed the samples in such conditions?
Regards Rashmi
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both takenomm-at-uw.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: takenomm-at-uw.edu Name: Marc Takeno
Organization: University of Washington
Title-Subject: [Filtered] [TEM] Experience (good/bad) with side-mounted Olympus MegaviewG2 and Barlow CMOS100
Message: Hello,
We are considering purchasing a side-mounted TEM camera system for our JEOL.
The candidates are:
Olympus Megaview G2 Barlow CMOS 100
If you have experiences good/bad with the above mentioned cameras, including software and ease-of-use, please let me know! Thank you.
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==============================Original Headers============================== 15, 41 -- From nestor.zaluzec-at-gmail.com Wed Oct 1 16:46:28 2014 15, 41 -- Received: from mail-wg0-f46.google.com (mail-wg0-f46.google.com [74.125.82.46]) 15, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s91LkRFH019382 15, 41 -- for {microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 16:46:27 -0500 15, 41 -- Received: by mail-wg0-f46.google.com with SMTP id k14so1770144wgh.29 15, 41 -- for {microscopy-at-microscopy.com} ; Wed, 01 Oct 2014 14:46:27 -0700 (PDT) 15, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 41 -- d=gmail.com; s=20120113; 15, 41 -- h=sender:from:message-id:date:reply-to:user-agent:mime-version:to 15, 41 -- :subject:references:in-reply-to:content-type 15, 41 -- :content-transfer-encoding; 15, 41 -- bh=245fqzyqgzH1xZj9X88NvQwG1WInKU2fD0wNMLWkwFw=; 15, 41 -- b=cuwGgLFKzP10NYXUbwWAqCpTlQR/nff1ygjEieWxjFOOMjLIHmEO6+Cfyav3UeRUKz 15, 41 -- oV8Ebq+ANW9ZZHxDWhzt/IGMuP5kzyCl3lUNbgfhLua8kGRIGxB5Jr0nvxMoz+uEmNoU 15, 41 -- Fjg0biG47iNdDncQGb1rqdFkZ/a83PVMAqqxRaZOd626X6S5kjyOO+BvddPkdSA6lb3s 15, 41 -- OrAWGCS30ioPPWErqvoMMQNTho571VofN3gZ2K0SIXVrmoJhZIhMKVCmpJjsQNcfsMBN 15, 41 -- v4zoTPhXV5L2YXaMbhn6d8Nwo+88nbWTGwfbGfu4+k4V1KRRe/paYGrV4IGlFW4bGnlc 15, 41 -- Oe+w== 15, 41 -- X-Received: by 10.180.95.163 with SMTP id dl3mr18295612wib.79.1412199987058; 15, 41 -- Wed, 01 Oct 2014 14:46:27 -0700 (PDT) 15, 41 -- Received: from nestorsmbp2014.home (host31-51-214-242.range31-51.btcentralplus.com. [31.51.214.242]) 15, 41 -- by mx.google.com with ESMTPSA id d6sm3176916wiz.0.2014.10.01.14.46.26 15, 41 -- for {microscopy-at-microscopy.com} 15, 41 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 15, 41 -- Wed, 01 Oct 2014 14:46:26 -0700 (PDT) 15, 41 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 15, 41 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 15, 41 -- X-Google-Original-From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 41 -- Message-ID: {542C7635.9040302-at-microscopy.com} 15, 41 -- Date: Wed, 01 Oct 2014 22:46:29 +0100 15, 41 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 41 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 15, 41 -- MIME-Version: 1.0 15, 41 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 41 -- Subject: viaWWW: [TEM] Experience (good/bad) with side-mounted Olympus MegaviewG2 15, 41 -- and Barlow CMOS100 15, 41 -- References: {201410011802.s91I2Egd014137-at-ns.microscopy.com} 15, 41 -- In-Reply-To: {201410011802.s91I2Egd014137-at-ns.microscopy.com} 15, 41 -- X-Forwarded-Message-Id: {201410011802.s91I2Egd014137-at-ns.microscopy.com} 15, 41 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From gaiihti-at-rr.com Wed Oct 1 17:29:10 2014 Return-Path: {gaiihti-at-rr.com} Received: from cpe-108-183-197-190.twcny.res.rr.com (cpe-108-183-197-190.twcny.res.rr.com [108.183.197.190]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s91MT924003699 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 1 Oct 2014 17:29:09 -0500 Message-ID: {F51993F0.6480386-at-rr.com}
Hi, Jon
Encourage her to investigate becoming a technical applications specialist. I started out my early days in this area and, over the intervening decades have found writing to be an invaluable skill to both my career development and my employers. I still write avidly (visit The Library at www.MicroscopyEducation.com) and have found that my writing always takes me into new and interesting applications and areas of technology.
Good hunting! Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com
*A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277
MME is currently scheduling courses for now and through Jan 2015. Call us today for a free training evaluation.
At 03:09 PM 10/1/2014, jkrupp-at-deltacollege.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 28 -- From bfoster-at-the-mip.com Wed Oct 1 18:37:28 2014 9, 28 -- Received: from barcelona.directrouter.com (barcelona.directrouter.com [72.249.107.2]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s91NbStV004353 9, 28 -- for {Microscopy-at-Microscopy.com} ; Wed, 1 Oct 2014 18:37:28 -0500 9, 28 -- Message-Id: {201410012337.s91NbStV004353-at-ns.microscopy.com} 9, 28 -- Received: from 99-168-104-53.lightspeed.rcsntx.sbcglobal.net ([99.168.104.53]:60947 helo=Bs-PC.the-mip.com) 9, 28 -- by barcelona.directrouter.com with esmtpsa (TLSv1:DHE-RSA-AES256-SHA:256) 9, 28 -- (Exim 4.82) 9, 28 -- (envelope-from {bfoster-at-the-mip.com} ) 9, 28 -- id 1XZTSY-0003p4-PW; Wed, 01 Oct 2014 18:37:27 -0500 9, 28 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 28 -- Date: Wed, 01 Oct 2014 18:37:24 -0500 9, 28 -- To: jkrupp-at-deltacollege.edu, Microscopy-at-Microscopy.com 9, 28 -- From: Barbara Foster {bfoster-at-the-mip.com} 9, 28 -- Subject: Re: [Microscopy] Technical writing 9, 28 -- In-Reply-To: {201410012000.s91K0sig001042-at-ns.microscopy.com} 9, 28 -- References: {201410012000.s91K0sig001042-at-ns.microscopy.com} 9, 28 -- Mime-Version: 1.0 9, 28 -- Content-Type: text/plain; charset="us-ascii" 9, 28 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 9, 28 -- X-AntiAbuse: Primary Hostname - barcelona.directrouter.com 9, 28 -- X-AntiAbuse: Original Domain - microscopy.com 9, 28 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 9, 28 -- X-AntiAbuse: Sender Address Domain - the-mip.com 9, 28 -- X-Get-Message-Sender-Via: barcelona.directrouter.com: authenticated_id: bfoster-at-the-mip.com 9, 28 -- X-Source: 9, 28 -- X-Source-Args: 9, 28 -- X-Source-Dir: ==============================End of - Headers==============================
From express-meds13-at-oneonta.k12.al.us Wed Oct 1 20:17:52 2014 Return-Path: {express-meds13-at-oneonta.k12.al.us} Received: from smtp4.oneonta.k12.al.us ([192.162.112.138]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s921HdoJ025302 for {microscopylistserverarchive2-at-microscopy.com} ; Wed, 1 Oct 2014 20:17:41 -0500 Message-Id: {201410020117.s921HdoJ025302-at-ns.microscopy.com}
X-from: hwitkiewicz-at-ucsd.edu ()
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Title-Subject: [Filtered] Re: [TEM] Experience (good/bad) with side-mounted Olympus MegaviewG2 and Barlow CMOS100
Message: Hi Marc,
My experience with side-mounted Olympus Megaview III camera was GOOD. Please see http://f1000research.com/search?q=witkiewicz ('Viewing' section in any of the three articles listed there).
Regards, Halina
Email: takenomm-at-uw.edu Name: Marc Takeno
Organization: University of Washington
Title-Subject: [Filtered] [TEM] Experience (good/bad) with side-mounted Olympus MegaviewG2 and Barlow CMOS100
Message: Hello,
We are considering purchasing a side-mounted TEM camera system for our JEOL.
The candidates are:
Olympus Megaview G2 Barlow CMOS 100
If you have experiences good/bad with the above mentioned cameras, including software and ease-of-use, please let me know! Thank you.
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What kind of conditions are needed to deposit a film of thickness of about 10 nm by evaporation of a tungsten wire in high vacuum by resistive heating? What level of vacuum is necessary to avoid oxidation, what kind of power supply, W wire thickness etc. parameters are needed? What would be the approximate rate of deposition?
Krassimir Bozhilov.
==============================Original Headers============================== 2, 33 -- From krassimir.bozhilov-at-ucr.edu Thu Oct 2 11:30:02 2014 2, 33 -- Received: from mx1.ucr.edu (mx1.ucr.edu [138.23.248.2]) 2, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s92GU1AE028567 2, 33 -- for {microscopy-at-microscopy.com} ; Thu, 2 Oct 2014 11:30:01 -0500 2, 33 -- X-IronPort-Anti-Spam-Filtered: true 2, 33 -- X-IronPort-Anti-Spam-Result: AhICAEt9LVSKF/hAnGdsb2JhbABghD7TPRYBEQEBAQEBCAsJCRQjCYQKOlEBPkInBIhRBZgOnwKGWY9YhAOBHQWPU4MglzqMXII0gQIBAQE 2, 33 -- X-IronPort-AV: E=Sophos;i="5.04,639,1406617200"; 2, 33 -- d="scan'208";a="266850349" 2, 33 -- Received: from exch-edge-2.ucr.edu ([138.23.248.64]) 2, 33 -- by smtp1.ucr.edu with ESMTP/TLS/AES128-SHA; 02 Oct 2014 09:29:25 -0700 2, 33 -- Received: from EXCH-HT-2.exch.ucr.edu (138.23.248.67) by exch-edge-2.ucr.edu 2, 33 -- (138.23.248.64) with Microsoft SMTP Server (TLS) id 14.3.195.1; Thu, 2 Oct 2, 33 -- 2014 09:29:22 -0700 2, 33 -- Received: from EXCH-MBOX-6.exch.ucr.edu ([169.254.6.209]) by 2, 33 -- EXCH-HT-2.exch.ucr.edu ([138.23.248.67]) with mapi id 14.03.0195.001; Thu, 2 2, 33 -- Oct 2014 09:29:25 -0700 2, 33 -- From: Krassimir Bozhilov {krassimir.bozhilov-at-ucr.edu} 2, 33 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 33 -- Subject: Tungsten evaporation 2, 33 -- Thread-Topic: Tungsten evaporation 2, 33 -- Thread-Index: AQHP3l4GZDZq/F2C9E6XnR/X87k/iw== 2, 33 -- Date: Thu, 2 Oct 2014 16:29:23 +0000 2, 33 -- Message-ID: {F7B25DF4-7E48-4668-B295-A2C3B573D54F-at-ucr.edu} 2, 33 -- Accept-Language: en-US 2, 33 -- Content-Language: en-US 2, 33 -- X-MS-Has-Attach: 2, 33 -- X-MS-TNEF-Correlator: 2, 33 -- x-originating-ip: [138.23.63.83] 2, 33 -- Content-Type: text/plain; charset="us-ascii" 2, 33 -- Content-ID: {8EEC6701DB5FA245A48F518EB28294FD-at-exch.ucr.edu} 2, 33 -- MIME-Version: 1.0 2, 33 -- Content-Transfer-Encoding: 8bit 2, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s92GU1AE028567 ==============================End of - Headers==============================
I would guess that there are a number of reasons why you have this problem, others may help here? However, provided you have stable emission and the focus is also stable will have no problems with your work.
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
X-from: rashmi_mehata-at-yahoo.com ()
Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: DBT
Title-Subject: [Filtered] error message
Message: Hi!!
The FEI Quanta 200 showing the message "filament blown off" on the contrary they are able to see the filament emission but the filament emission signal turns from green to red. The error occurs after some time when the HT and filament was switched on. It is almost 3-4 four months now they are getting such erratic problems. Not able to trace out the reason for this. Is it suitable to analyzed the samples in such conditions?
Regards Rashmi
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==============================Original Headers============================== 18, 40 -- From nestor.zaluzec-at-gmail.com Wed Oct 1 16:43:23 2014 18, 40 -- Received: from mail-wi0-f171.google.com (mail-wi0-f171.google.com [209.85.212.171]) 18, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s91LhM8f011681 18, 40 -- for {microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 16:43:23 -0500 18, 40 -- Received: by mail-wi0-f171.google.com with SMTP id ho1so2069222wib.10 18, 40 -- for {microscopy-at-microscopy.com} ; Wed, 01 Oct 2014 14:43:22 -0700 (PDT) 18, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 18, 40 -- d=gmail.com; s=20120113; 18, 40 -- h=sender:from:message-id:date:reply-to:user-agent:mime-version:to 18, 40 -- :subject:references:in-reply-to:content-type 18, 40 -- :content-transfer-encoding; 18, 40 -- bh=mqqnvflYN2flex7xcq4bKqsoRtxJlwbI0N2xtsMXmOI=; 18, 40 -- b=0G1b6hqEbS8KNEiKK0E4Hx0mdyPgOtod2Z8xyWFOjtDIDvMMfEBwUbvoXD/rGZOGzj 18, 40 -- IWxJNU3GajOzNtlbYmiBHxMjdvSpa8QtAHN8Ze5cvDQJqzbBJgXCRzeN6KreYZe1P4HP 18, 40 -- dXwm6yWE2pZHZ8hJVuGbv3P6AlsIaxwGwxnRMeGTC85n00YSfNSATvC4qRFAtdyGFh/Z 18, 40 -- Xn25x/2YeRJ/kgK08c/NfXgfxocerhIqbtWenJmQ6J7MrUojv5ZQfwBwaP8QXMYA6C35 18, 40 -- QeC8EVbiM7n8T940/gqoV+XyfU3TEZqwpoFaeg7DOhdmcfncnx0VNxfIr6XABv3SCyZs 18, 40 -- 4Bfw== 18, 40 -- X-Received: by 10.180.101.70 with SMTP id fe6mr5345232wib.37.1412199802228; 18, 40 -- Wed, 01 Oct 2014 14:43:22 -0700 (PDT) 18, 40 -- Received: from nestorsmbp2014.home (host31-51-214-242.range31-51.btcentralplus.com. [31.51.214.242]) 18, 40 -- by mx.google.com with ESMTPSA id xm4sm3158851wib.9.2014.10.01.14.43.21 18, 40 -- for {microscopy-at-microscopy.com} 18, 40 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 18, 40 -- Wed, 01 Oct 2014 14:43:21 -0700 (PDT) 18, 40 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 18, 40 -- X-from: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 18, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 40 -- Message-ID: {542C757C.1000709-at-microscopy.com} 18, 40 -- Date: Wed, 01 Oct 2014 22:43:24 +0100 18, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 18, 40 -- MIME-Version: 1.0 18, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 40 -- Subject: viaWWW: Filament error message 18, 40 -- References: {201410011629.s91GToDg031286-at-ns.microscopy.com} 18, 40 -- In-Reply-To: {201410011629.s91GToDg031286-at-ns.microscopy.com} 18, 40 -- X-Forwarded-Message-Id: {201410011629.s91GToDg031286-at-ns.microscopy.com} 18, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 23, 22 -- From protrain-at-emcourses.com Thu Oct 2 21:25:17 2014 23, 22 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 23, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s932PGSu026907 23, 22 -- for {microscopy-at-microscopy.com} ; Thu, 2 Oct 2014 21:25:16 -0500 23, 22 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 23, 22 -- id {B542e08fb0000} ; Fri, 03 Oct 2014 03:24:59 +0100 23, 22 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Fri, 3 Oct 2014 03:24:58 +0100 23, 22 -- Reply-To: {protrain-at-emcourses.com} 23, 22 -- From: "Steve Chapman" {protrain-at-emcourses.com} 23, 22 -- To: {rashmi_mehata-at-yahoo.com} 23, 22 -- Cc: "'Microscopical Soc of America'" {microscopy-at-microscopy.com} 23, 22 -- Subject: FW: [Microscopy] viaWWW: Filament error message 23, 22 -- Date: Fri, 3 Oct 2014 03:25:13 +0100 23, 22 -- Organization: Protrain 23, 22 -- Message-ID: {000c01cfdeb1$43773fe0$ca65bfa0$-at-com} 23, 22 -- MIME-Version: 1.0 23, 22 -- Content-Type: text/plain; 23, 22 -- charset="us-ascii" 23, 22 -- Content-Transfer-Encoding: 7bit 23, 22 -- X-Mailer: Microsoft Office Outlook 12.0 23, 22 -- Thread-Index: Ac/dwNaMEwvSuRd+RVqCKjG2RwNFpwAa6a9gACEhF/A= 23, 22 -- Content-Language: en-gb ==============================End of - Headers==============================
From kjooiiio-at-memoryplay.com Fri Oct 3 04:22:19 2014 Return-Path: {kjooiiio-at-memoryplay.com} Received: from mail1.memoryplay.com ([217.26.10.226]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s939MIO6023085 for {microscopylistserverarchive2-at-microscopy.com} ; Fri, 3 Oct 2014 04:22:19 -0500 Message-ID: {BBAB8827.8945693-at-memoryplay.com}
I did not have a change to dig into Quanta 200, but based on typical architecture of SEM detection of blown filament should be made by the high voltage power supply (HVPS) - usually a pricey item to repair.
Can't be sure of exact multifunction, but if parameters of filament current, extractor/anode currents, and the image are stable, then I'd run it until the HVPS quits completely.
Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/2/2014 10:26 PM, protrain-at-emcourses.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } } I would guess that there are a number of reasons why you have this problem, } others may help here? However, provided you have stable emission and the } focus is also stable will have no problems with your work. } } Steve } } Steve Chapman FRMS } Protrain for Consultancy and Courses in Electron Microscopy } Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 } www.emcourses.com } } } X-from: rashmi_mehata-at-yahoo.com () } } Email: rashmi_mehata-at-yahoo.com } Name: Rashmi } } Organization: DBT } } Title-Subject: [Filtered] error message } } Message: Hi!! } } The FEI Quanta 200 showing the message "filament blown off" on the contrary } they are able to see the filament emission but the filament emission signal } turns from green to red. } The error occurs after some time when the HT and filament was switched on. } It is almost 3-4 four months now they are getting such erratic problems. } Not able to trace out the reason for this. } Is it suitable to analyzed the samples in such conditions? } } Regards } Rashmi } } } } Login Host: 59.163.216.167 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } -- } =============================================== } } Do not reply to this messages it is from the Microscopy Listserver NO-REPLY } forwarding system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================= } } ==============================Original Headers============================== } 18, 40 -- From nestor.zaluzec-at-gmail.com Wed Oct 1 16:43:23 2014 18, 40 -- } Received: from mail-wi0-f171.google.com (mail-wi0-f171.google.com } [209.85.212.171]) } 18, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } s91LhM8f011681 } 18, 40 -- for {microscopy-at-microscopy.com} ; Wed, 1 Oct 2014 16:43:23 } -0500 } 18, 40 -- Received: by mail-wi0-f171.google.com with SMTP id } ho1so2069222wib.10 } 18, 40 -- for {microscopy-at-microscopy.com} ; Wed, 01 Oct 2014 14:43:22 } -0700 (PDT) } 18, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 40 -- d=gmail.com; s=20120113; } 18, 40 -- } h=sender:from:message-id:date:reply-to:user-agent:mime-version:to } 18, 40 -- :subject:references:in-reply-to:content-type } 18, 40 -- :content-transfer-encoding; } 18, 40 -- bh=mqqnvflYN2flex7xcq4bKqsoRtxJlwbI0N2xtsMXmOI=; } 18, 40 -- } b=0G1b6hqEbS8KNEiKK0E4Hx0mdyPgOtod2Z8xyWFOjtDIDvMMfEBwUbvoXD/rGZOGzj } 18, 40 -- } IWxJNU3GajOzNtlbYmiBHxMjdvSpa8QtAHN8Ze5cvDQJqzbBJgXCRzeN6KreYZe1P4HP } 18, 40 -- } dXwm6yWE2pZHZ8hJVuGbv3P6AlsIaxwGwxnRMeGTC85n00YSfNSATvC4qRFAtdyGFh/Z } 18, 40 -- } Xn25x/2YeRJ/kgK08c/NfXgfxocerhIqbtWenJmQ6J7MrUojv5ZQfwBwaP8QXMYA6C35 } 18, 40 -- } QeC8EVbiM7n8T940/gqoV+XyfU3TEZqwpoFaeg7DOhdmcfncnx0VNxfIr6XABv3SCyZs } 18, 40 -- 4Bfw== } 18, 40 -- X-Received: by 10.180.101.70 with SMTP id } fe6mr5345232wib.37.1412199802228; } 18, 40 -- Wed, 01 Oct 2014 14:43:22 -0700 (PDT) } 18, 40 -- Received: from nestorsmbp2014.home } (host31-51-214-242.range31-51.btcentralplus.com. [31.51.214.242]) } 18, 40 -- by mx.google.com with ESMTPSA id } xm4sm3158851wib.9.2014.10.01.14.43.21 } 18, 40 -- for {microscopy-at-microscopy.com} } 18, 40 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); } 18, 40 -- Wed, 01 Oct 2014 14:43:21 -0700 (PDT) } 18, 40 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com} 18, 40 -- } X-from: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com} 18, 40 -- } X-Google-Original-From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 18, 40 -- Message-ID: {542C757C.1000709-at-microscopy.com} 18, 40 -- Date: Wed, } 01 Oct 2014 22:43:24 +0100 18, 40 -- Reply-To: } microscopylistserver-noreply-at-microscopy.com } 18, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) } Gecko/20100101 Thunderbird/24.6.0 18, 40 -- MIME-Version: 1.0 18, 40 -- To: } MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 40 -- Subject: } viaWWW: Filament error message 18, 40 -- References: } {201410011629.s91GToDg031286-at-ns.microscopy.com} } 18, 40 -- In-Reply-To: {201410011629.s91GToDg031286-at-ns.microscopy.com} } 18, 40 -- X-Forwarded-Message-Id: } {201410011629.s91GToDg031286-at-ns.microscopy.com} } 18, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 40 } -- Content-Transfer-Encoding: 7bit ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 23, 22 -- From protrain-at-emcourses.com Thu Oct 2 21:25:17 2014 } 23, 22 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) } 23, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s932PGSu026907 } 23, 22 -- for {microscopy-at-microscopy.com} ; Thu, 2 Oct 2014 21:25:16 -0500 } 23, 22 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway } 23, 22 -- id {B542e08fb0000} ; Fri, 03 Oct 2014 03:24:59 +0100 } 23, 22 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Fri, 3 Oct 2014 03:24:58 +0100 } 23, 22 -- Reply-To: {protrain-at-emcourses.com} } 23, 22 -- From: "Steve Chapman" {protrain-at-emcourses.com} } 23, 22 -- To: {rashmi_mehata-at-yahoo.com} } 23, 22 -- Cc: "'Microscopical Soc of America'" {microscopy-at-microscopy.com} } 23, 22 -- Subject: FW: [Microscopy] viaWWW: Filament error message } 23, 22 -- Date: Fri, 3 Oct 2014 03:25:13 +0100 } 23, 22 -- Organization: Protrain } 23, 22 -- Message-ID: {000c01cfdeb1$43773fe0$ca65bfa0$-at-com} } 23, 22 -- MIME-Version: 1.0 } 23, 22 -- Content-Type: text/plain; } 23, 22 -- charset="us-ascii" } 23, 22 -- Content-Transfer-Encoding: 7bit } 23, 22 -- X-Mailer: Microsoft Office Outlook 12.0 } 23, 22 -- Thread-Index: Ac/dwNaMEwvSuRd+RVqCKjG2RwNFpwAa6a9gACEhF/A= } 23, 22 -- Content-Language: en-gb } ==============================End of - Headers============================== }
It appears our HT tank on our CM12 needs replacing. Has anyone ever had to replace or self service by opening up the tank and checking/replacing bad capacitors? Any suggestions specific for the CM12 would be appreciated
Fred Hayes Manager, AMCaT Labs Univ of CA Davis Dept Chem Engr and Mat Sci 3001 Ghausi Hall 1 Shields Ave Davis, CA 95616-5270 http://chms.engineering.ucdavis.edu/research/amcat/
==============================Original Headers============================== 4, 24 -- From fahayes-at-ucdavis.edu Fri Oct 3 13:41:27 2014 4, 24 -- Received: from msa2.ucdavis.edu (msa2.ucdavis.edu [128.120.32.182]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s93IfRcl018576 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Oct 2014 13:41:27 -0500 4, 24 -- Received: from CHMSK011201 (chms-3010a.engr.ucdavis.edu [169.237.64.45]) 4, 24 -- (authenticated bits=0) 4, 24 -- by msa2.ucdavis.edu (8.14.4/8.14.5/it-oel6-mimedefang-smtp-1.9) with ESMTP id s93IfOFI014809 4, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Oct 2014 11:41:25 -0700 4, 24 -- From: "Fred Hayes" {fahayes-at-ucdavis.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: CM12 TEM HT tank troubleshooting 4, 24 -- Date: Fri, 3 Oct 2014 11:41:24 -0700 4, 24 -- Message-ID: {000001cfdf39$a32eb1b0$e98c1510$-at-ucdavis.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="us-ascii" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Mailer: Microsoft Outlook 15.0 4, 24 -- Thread-Index: Ac/fOVMKrgonfiOyRySuahd52ooQNw== 4, 24 -- Content-Language: en-us 4, 24 -- X-Virus-Scanned: clamav-milter 0.98.1 at msa2 4, 24 -- X-Virus-Status: Clean 4, 24 -- X-Scanned-By: MIMEDefang 2.74 ==============================End of - Headers==============================
Electropolishing refractory metals, particularly tungsten, has a reputation as non-trivial. The literature consensus is a weak NaOH solution, but consensus degenerates after that.
Does anyone have advice for techniques and/or hardware? My interest is both bulk SEM electropolishing and 3 mm twin-jet for TEM.
Vendor responses welcome.
Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 9, 39 -- From parishcm-at-ornl.gov Fri Oct 3 17:57:11 2014 9, 39 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.14]) 9, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s93MvAes027952 9, 39 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Oct 2014 17:57:10 -0500 9, 39 -- X-SG: RELAYLIST 9, 39 -- X-IronPort-AV: E=Sophos;i="5.04,650,1406606400"; 9, 39 -- d="scan'208";a="86888541" 9, 39 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 9, 39 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 03 Oct 2014 18:57:10 -0400 9, 39 -- Received: from EXCHOS32.ornl.gov (exchos32.ornl.gov [128.219.12.152]) 9, 39 -- (using TLSv1 with cipher AES256-SHA (256/256 bits)) 9, 39 -- (No client certificate requested) 9, 39 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 12766E1244 9, 39 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Oct 2014 18:57:10 -0400 (EDT) 9, 39 -- Received: from EXCHCS33.ornl.gov (128.219.12.154) by EXCHOS32.ornl.gov 9, 39 -- (128.219.12.152) with Microsoft SMTP Server (TLS) id 15.0.847.32; Fri, 3 Oct 9, 39 -- 2014 18:57:03 -0400 9, 39 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS33.ornl.gov 9, 39 -- (128.219.12.147) with Microsoft SMTP Server (TLS) id 15.0.847.32; Fri, 3 Oct 9, 39 -- 2014 18:57:02 -0400 9, 39 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by 9, 39 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id 9, 39 -- 15.00.0847.030; Fri, 3 Oct 2014 18:57:02 -0400 9, 39 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 9, 39 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 9, 39 -- Subject: Electropolishing of tungsten 9, 39 -- Thread-Topic: Electropolishing of tungsten 9, 39 -- Thread-Index: Ac/fXVQawUDqFrcDSwqn+EoIQ45Wmg== 9, 39 -- Date: Fri, 3 Oct 2014 22:57:01 +0000 9, 39 -- Message-ID: {7ad6d01b4356449b9441f4c6aa2b81e1-at-EXCHCS32.ornl.gov} 9, 39 -- Accept-Language: en-US 9, 39 -- Content-Language: en-US 9, 39 -- X-MS-Has-Attach: 9, 39 -- X-MS-TNEF-Correlator: 9, 39 -- x-originating-ip: [128.219.12.132] 9, 39 -- Content-Type: text/plain; charset="us-ascii" 9, 39 -- MIME-Version: 1.0 9, 39 -- Content-Transfer-Encoding: 8bit 9, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s93MvAes027952 ==============================End of - Headers==============================
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Email: Richard.Gursky-at-stjude.org Name: Richard Gursky
Organization: St. Jude ChildrenÂ’s Research Hospital
Title-Subject: [Filtered] User Friendly Laboratory Information Management System (LIMS)
Message: User Friendly Laboratory Information Management System.
We are looking to replace our curret Laboratory Information Management System (LIMS) for a more User Friendly LIMS (read as takes less time for Sample Logging & Cost Center Charging). Would any of you recommend the system that you work with? Could someone give me any information on the LIMS that you use and could you put me in touch with your IT guys so we could get some information from them?
Thanks Much!
Richard Gursky Cellular Imaging Gp. St. Jude ChildrenÂ’s Research Hospital 262 Danny Thomas Place, MS 330 Memphis, TN 38105 901-595-2912 or 901-595-6497
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Email: j.knowles-at-ucl.ac.uk Name: Jonathan
Organization: UCL
Title-Subject: [Filtered] TEM camera software
Message: Hi
We have a Hamamatsu camera and were running the AMT imaging software for image capture. Both pretty old verions on Xp!
The PC has died and we needed to reinstall the software, but AMT are quoting a hefty price to come in and install it.
Would anyone by any chance know where i could get a copy at a reasonable price or free?
thanks
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Question: What is the approximate response time of the phosphor screen (viewing green screen), which I believe is commonly coated with Zinc Sulphide? I need to know the rise and decay time of ZnS fluorescence. Is there a way for me to characterize this response time?
Also, can this response time be varied with different coatings or by varying the intensity of the beam?
Thank you in advance,
Raj.
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From reykarpu-at-jajagroup.com Sat Oct 4 09:45:45 2014 Return-Path: {reykarpu-at-jajagroup.com} Received: from mail2.jajagroup.com ([91.205.131.3]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s94EjinJ026482 for {microscopylistserverarchive2-at-microscopy.com} ; Sat, 4 Oct 2014 09:45:44 -0500 Message-ID: {F21C2DD9.3552688-at-jajagroup.com}
Greetings Microscopists!
Recently I have been informed that Leica will no longer be carrying the 200 micron membrane carriers for their EM-Pact(1 or 2) High Pressure Freezer. I am wondering if anyone has some laying around that they would be willing to sell or give away because they are no longer using? Or maybe they have something better that they use as a replacement.
Has anyone else encountered this problem, and do they have a solution?
Cheers!
Rhonda Trimble Ross Electron Microscopy Specialist Stowers Institute 1000 E 50th St Kansas City, MO 64110 rra-at-stowers.org
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Email: meb187-at-pitt.edu Name: Margaret Bisher
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Title-Subject: [Filtered] Forma Scientific Incubator, Model 3546
Message: We have inherited this incubator and I was wondering if anyone might have the manual.
Thanks for checking.
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Email: Brian.whelan-at-theremigroup.com Name: Brian Whelan
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Title-Subject: [Filtered] FEI Tecnai 12BT
Message: Would any 3rd party be interested in providing a full service quote for FEI Tecnai 12BT? The quote would include parts,PM and labor. This unit is located in NY. Thank you in advanced
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Email: emurray-at-asgireland.com Name: Emma Murray
Organization: University of Ulster
Title-Subject: [Filtered] Research Fellow in Characterisation of Metal Oxides for Electronic Applications
Message: University of Ulster
Nanotechnology and Integrated BioEngineering Centre Research Fellow in Characterisation of Metal Oxides for Electronic Applications
Ref: 1437106 Salary: Ł39,684 Base: Jordanstown
Closing date: 31 October 2014
The postholder will work on a collaborative project between the Nanotechnology and Integrated BioEngineering Centre (NIBEC) and AVX Ltd.
Candidates must have a strong track record in the characterisation of solid state materials using TEM, XRD and other advanced analytical methods. A knowledge and understanding of the role of defects and dopants in controlling the electrical properties of materials is essential.
This project will involve experimental research on the increased reliability and performance of multi-layer ceramic capacitors and will advance knowledge in relation to the fundamental physico-chemical and electronic properties of MLCC materials and devices. (Fixed-term until 31 March 2018)
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From gaiweji-at-tmodns.net Thu Oct 9 01:29:04 2014 Return-Path: {gaiweji-at-tmodns.net} Received: from me25636d0.tmodns.net ([172.56.6.78]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s996StOY018427 for {microscopylistserverarchive2-at-microscopy.com} ; Thu, 9 Oct 2014 01:28:57 -0500 Message-ID: {7F0C7BCD.2938393-at-tmodns.net}
Just curious. How essential is it to prepare CO2 free water for mixing and using lead citrate stain?
I have always done it, but wonder if anyone has tried skipping it.
How about alternate ways to prepare it. Boiling is the standard, but anyone boil using microwave or I have heard sonicating might work too.
Sitting here with a scalding flask of water, wondering if I can cheat the EM gods.
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 16, 38 -- From prvs=13532e5fac=jkrupp-at-deltacollege.edu Fri Oct 10 14:45:21 2014 16, 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.237]) 16, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id s9AJjKjK020464 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 14:45:20 -0500 16, 38 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 16, 38 -- by localhost (Email Security Appliance) with SMTP id 5CADDA29291_438374DB 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 19:45:17 +0000 (GMT) 16, 38 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) 16, 38 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 5724A9B04E9_438374DF 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 19:45:17 +0000 (GMT) 16, 38 -- Received: from localhost (localhost [127.0.0.1]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5306DF80DF41 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 12:45:17 -0700 (PDT) 16, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 16, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 16, 38 -- with ESMTP id DilPWkf6EYZx for {Microscopy-at-microscopy.com} ; 16, 38 -- Fri, 10 Oct 2014 12:45:12 -0700 (PDT) 16, 38 -- Received: from localhost (localhost [127.0.0.1]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 00DF1FB285F1 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 12:45:11 -0700 (PDT) 16, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 16, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 16, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 16, 38 -- with ESMTP id X2thFYA8pwa2 for {Microscopy-at-microscopy.com} ; 16, 38 -- Fri, 10 Oct 2014 12:45:11 -0700 (PDT) 16, 38 -- Received: from [10.146.0.11] (unknown [10.146.0.11]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id D8C70F80DF41 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 12:45:11 -0700 (PDT) 16, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 16, 38 -- Content-Type: text/plain; charset=us-ascii 16, 38 -- Subject: CO2 free water 16, 38 -- Date: Fri, 10 Oct 2014 12:45:11 -0700 16, 38 -- Message-Id: {B80D321B-694C-4D59-8CBB-A507807107BF-at-deltacollege.edu} 16, 38 -- To: Microscopy-at-microscopy.com 16, 38 -- Mime-Version: 1.0 (Apple Message framework v1085) 16, 38 -- X-Mailer: Apple Mail (2.1085) 16, 38 -- Content-Transfer-Encoding: 8bit 16, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9AJjKjK020464 ==============================End of - Headers==============================
Jon - What is the downside of doing this just to be sure? There aren't many steps in science easier than "boil water". I have used a microwave but to ensure long enough boiling, it makes it a little messier since the water spills over so a hot plate is probably worth the effort. I am sure sonication works but that is more trouble than needed since you don't have to worry about evaporative loss or flammability when you boil water (unless your lab is adjacent to a fracking well). I have used sonication in the steps involving getting lead nitrate or lead citrate to go into solution but only since I am so compulsive. Could you cheat the gods and skip this step? Sure, 99 times out of a 100. But the downstream problems aren't worth the risk. If you made a batch and got staining artefacts, how would you know if you screwed up the staining step or the error was in not originally boiling the water. I am as frugal and lazy as the next guy but this is as cheap and easy as it gets in science. Find something else to eliminate in your life. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Friday, October 10, 2014 2:47 PM To: Phillips, Thomas E.
Just curious. How essential is it to prepare CO2 free water for mixing and using lead citrate stain?
I have always done it, but wonder if anyone has tried skipping it.
How about alternate ways to prepare it. Boiling is the standard, but anyone boil using microwave or I have heard sonicating might work too.
Sitting here with a scalding flask of water, wondering if I can cheat the EM gods.
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 16, 38 -- From prvs=13532e5fac=jkrupp-at-deltacollege.edu Fri Oct 10 14:45:21 2014 16, 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.237]) 16, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id s9AJjKjK020464 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 14:45:20 -0500 16, 38 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 16, 38 -- by localhost (Email Security Appliance) with SMTP id 5CADDA29291_438374DB 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 19:45:17 +0000 (GMT) 16, 38 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) 16, 38 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 5724A9B04E9_438374DF 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 19:45:17 +0000 (GMT) 16, 38 -- Received: from localhost (localhost [127.0.0.1]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5306DF80DF41 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 12:45:17 -0700 (PDT) 16, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 16, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 16, 38 -- with ESMTP id DilPWkf6EYZx for {Microscopy-at-microscopy.com} ; 16, 38 -- Fri, 10 Oct 2014 12:45:12 -0700 (PDT) 16, 38 -- Received: from localhost (localhost [127.0.0.1]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 00DF1FB285F1 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 12:45:11 -0700 (PDT) 16, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 16, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 16, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 16, 38 -- with ESMTP id X2thFYA8pwa2 for {Microscopy-at-microscopy.com} ; 16, 38 -- Fri, 10 Oct 2014 12:45:11 -0700 (PDT) 16, 38 -- Received: from [10.146.0.11] (unknown [10.146.0.11]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id D8C70F80DF41 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 12:45:11 -0700 (PDT) 16, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 16, 38 -- Content-Type: text/plain; charset=us-ascii 16, 38 -- Subject: CO2 free water 16, 38 -- Date: Fri, 10 Oct 2014 12:45:11 -0700 16, 38 -- Message-Id: {B80D321B-694C-4D59-8CBB-A507807107BF-at-deltacollege.edu} 16, 38 -- To: Microscopy-at-microscopy.com 16, 38 -- Mime-Version: 1.0 (Apple Message framework v1085) 16, 38 -- X-Mailer: Apple Mail (2.1085) 16, 38 -- Content-Transfer-Encoding: 8bit 16, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9AJjKjK020464 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 31 -- From PhillipsT-at-missouri.edu Fri Oct 10 16:10:51 2014 26, 31 -- Received: from um-tip1-missouri-out.um.umsystem.edu (um-tip1-missouri-out.um.umsystem.edu [198.209.49.135]) 26, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9ALAoEo009296 26, 31 -- for {microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 16:10:50 -0500 26, 31 -- X-IronPort-Anti-Spam-Filtered: true 26, 31 -- X-IronPort-Anti-Spam-Result: Ah4FAHtKOFTPoJ7S/2dsb2JhbABdAw6DAFNYBMsZCodNAoEFFgF7hAMBAQEEOhQ3AgICAQgRAwEBAQsCEgkHFhsBFAkIAgQBCQkIEQICBIgdDblRhhIBAQEBAQEBAQEBAQEBAQEBAQEBAQEXBI4rgTMQAgEeBhsSBQYLgiVTJIEeBYUVgRiLTINeZIg+PJApg36DN0BsAYFHgQIBAQE 26, 31 -- X-IPAS-Result: Ah4FAHtKOFTPoJ7S/2dsb2JhbABdAw6DAFNYBMsZCodNAoEFFgF7hAMBAQEEOhQ3AgICAQgRAwEBAQsCEgkHFhsBFAkIAgQBCQkIEQICBIgdDblRhhIBAQEBAQEBAQEBAQEBAQEBAQEBAQEXBI4rgTMQAgEeBhsSBQYLgiVTJIEeBYUVgRiLTINeZIg+PJApg36DN0BsAYFHgQIBAQE 26, 31 -- Received: from um-ncas5.um.umsystem.edu ([207.160.158.210]) 26, 31 -- by um-tip1-exch-relay.um.umsystem.edu with ESMTP; 10 Oct 2014 16:10:49 -0500 26, 31 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.90]) by 26, 31 -- UM-NCAS5.um.umsystem.edu ([207.160.158.210]) with mapi id 14.03.0210.002; 26, 31 -- Fri, 10 Oct 2014 16:10:49 -0500 26, 31 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 26, 31 -- To: "jkrupp-at-deltacollege.edu" {jkrupp-at-deltacollege.edu} , 26, 31 -- "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 26, 31 -- Subject: RE: [Microscopy] CO2 free water 26, 31 -- Thread-Topic: [Microscopy] CO2 free water 26, 31 -- Thread-Index: AQHP5ML+cmYAnrx3KEqyhTDeU9/trJwp0fEA 26, 31 -- Date: Fri, 10 Oct 2014 21:10:49 +0000 26, 31 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DDBF9FBDD-at-UM-MBX-T02.um.umsystem.edu} 26, 31 -- References: {201410101947.s9AJlFmx021362-at-ns.microscopy.com} 26, 31 -- In-Reply-To: {201410101947.s9AJlFmx021362-at-ns.microscopy.com} 26, 31 -- Accept-Language: en-US 26, 31 -- Content-Language: en-US 26, 31 -- X-MS-Has-Attach: 26, 31 -- X-MS-TNEF-Correlator: 26, 31 -- x-originating-ip: [128.206.81.115] 26, 31 -- Content-Type: text/plain; charset="us-ascii" 26, 31 -- MIME-Version: 1.0 26, 31 -- Content-Transfer-Encoding: 8bit 26, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9ALAoEo009296 ==============================End of - Headers==============================
I got an old Hitachi s-450 SEM with EDX from a friend when he upgraded to a Jeol 6300F.
It has some scan problems which I dont think will be much of an issue, the problem is I do not have any good filaments. The last good one he had blew not long ago and I am a bit reluctant to blow $500 on a box of filaments if it turns out to be a bigger problem than I think it is. Anyone know where I might find a seller that sells in individual quantities.
I have a couple bases to rebuild but I would like to find a couple more, I have a capacitive discharge spot welder to rebuild my own once I get some tungsten wire.
Thanks
-Jerry
==============================Original Headers============================== 5, 34 -- From jerry.biehler-at-gmail.com Fri Oct 10 22:34:27 2014 5, 34 -- Received: from mail-pa0-f49.google.com (mail-pa0-f49.google.com [209.85.220.49]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9B3YQQe001620 5, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 22:34:26 -0500 5, 34 -- Received: by mail-pa0-f49.google.com with SMTP id hz1so2749064pad.8 5, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 20:34:25 -0700 (PDT) 5, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 34 -- d=gmail.com; s=20120113; 5, 34 -- h=from:content-type:content-transfer-encoding:subject:message-id:date 5, 34 -- :to:mime-version; 5, 34 -- bh=GIcjsRLcNgcdfIkZxV8pYuTLdteMUhvrrzU/zBXIi4Q=; 5, 34 -- b=mE7i6oihyzeZwZ2Kw4Ui4cTZSm04Ty6PLERZgQGNO4MY3quyvzKLgru101zMDBO+Rc 5, 34 -- YRk+1YaTlFb4uT+r/dLQ9XnF/hx5dMe8HQqVeHn3CCgZdHsw5sByBbqsY3d7RdjU77+H 5, 34 -- Ys6lrM1oLmw3jCRizy5p9EFgI0HsRa+1C8GyYwfPqXErYNGt9OmDH6zamsEIEKHtQkM/ 5, 34 -- /azLcg10nhYyGwJkPLAjiKP+ohp8iZtD2IHNIapNTQN2OhxnthFNXBXq0qyMxgfr5+P1 5, 34 -- D/SgZuGkUd1uVERpqDLyuhDywfFH8D5IbGRYZxdud1xnK028a2acyRJXvTDDSQecyo3E 5, 34 -- 5gRw== 5, 34 -- X-Received: by 10.68.68.193 with SMTP id y1mr136191pbt.136.1412998465549; 5, 34 -- Fri, 10 Oct 2014 20:34:25 -0700 (PDT) 5, 34 -- Received: from [192.168.1.126] (static-50-53-104-123.bvtn.or.frontiernet.net. [50.53.104.123]) 5, 34 -- by mx.google.com with ESMTPSA id zn2sm4796021pbb.41.2014.10.10.20.34.24 5, 34 -- for {Microscopy-at-microscopy.com} 5, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 5, 34 -- Fri, 10 Oct 2014 20:34:24 -0700 (PDT) 5, 34 -- From: Jerry Biehler {jerry.biehler-at-gmail.com} 5, 34 -- Content-Type: text/plain; charset=us-ascii 5, 34 -- Subject: Hitachi Filaments 5, 34 -- Message-Id: {57719E1B-7594-40D8-98D9-7217D6D121F7-at-gmail.com} 5, 34 -- Date: Fri, 10 Oct 2014 20:34:23 -0700 5, 34 -- To: Microscopy-at-microscopy.com 5, 34 -- Mime-Version: 1.0 (Mac OS X Mail 8.0 \(1990.1\)) 5, 34 -- X-Mailer: Apple Mail (2.1990.1) 5, 34 -- Content-Transfer-Encoding: 8bit 5, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9B3YQQe001620 ==============================End of - Headers==============================
Dear Microscopy Managers and Directors Please share with your light and fluorescence microscopy core users
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January 12-16, 2015 University of California, Santa Barbara
Offered by the Neuroscience Research Institute (NRI) and Department of Molecular, Cellular, and Developmental Biology (MCDB). contact Mary Raven m_raven-at-lifesci.ucsb.edu for scholarship information and requests
See for workshop information: http://microscopy.nri.ucsb.edu/education/imaging-live-workshop/advanced-microscopy-live
==============================Original Headers============================== 5, 31 -- From mary.raven-at-lifesci.ucsb.edu Mon Oct 13 22:59:31 2014 5, 31 -- Received: from fed1rmfepo203.cox.net (fed1rmfepo203.cox.net [68.230.241.148]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9E3xUl9015332 5, 31 -- for {microscopy-at-microscopy.com} ; Mon, 13 Oct 2014 22:59:30 -0500 5, 31 -- Received: from fed1rmimpo210 ([68.230.241.161]) by fed1rmfepo203.cox.net 5, 31 -- (InterMail vM.8.01.05.15 201-2260-151-145-20131218) with ESMTP 5, 31 -- id {20141014035930.QPKB28942.fed1rmfepo203.cox.net-at-fed1rmimpo210} 5, 31 -- for {microscopy-at-microscopy.com} ; Mon, 13 Oct 2014 23:59:30 -0400 5, 31 -- Received: from Marys-iMac.local ([24.254.85.157]) 5, 31 -- by fed1rmimpo210 with cox 5, 31 -- id 2rzV1p00H3PgeUg01rzVzu; Mon, 13 Oct 2014 23:59:30 -0400 5, 31 -- X-CT-Class: Clean 5, 31 -- X-CT-Score: 0.00 5, 31 -- X-CT-RefID: str=0001.0A020203.543C9FA2.001B,ss=1,re=0.000,fgs=0 5, 31 -- X-CT-Spam: 0 5, 31 -- X-Authority-Analysis: v=2.0 cv=DLtRFVxb c=1 sm=1 5, 31 -- a=EaecXw6o90JchT8X97nwIQ==:17 a=NyMI_EmnuGIA:10 a=N659UExz7-8A:10 5, 31 -- a=zWc4U4XLAAAA:8 a=nicflVnj-7QI82GFZZkA:9 a=pILNOxqGKmIA:10 a=MH1D1_eGzVYA:10 5, 31 -- a=L5dUPsYGvU-ImAqD:21 a=2oCDPAWOwX9vCH1C:21 a=EaecXw6o90JchT8X97nwIQ==:117 5, 31 -- X-CM-Score: 0.00 5, 31 -- Authentication-Results: cox.net; none 5, 31 -- Message-ID: {543C9FA1.3020105-at-lifesci.ucsb.edu} 5, 31 -- Date: Mon, 13 Oct 2014 20:59:29 -0700 5, 31 -- From: Mary Raven {mary.raven-at-lifesci.ucsb.edu} 5, 31 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 5, 31 -- MIME-Version: 1.0 5, 31 -- To: Microscopy-at-microscopy.com 5, 31 -- Subject: LM - Scholarships are available - Microscopy Workshop Jan 12-16 workshop 5, 31 -- in Santa Barbara, CA 5, 31 -- Content-Type: text/plain; charset=windows-1252; format=flowed 5, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Agreeing with Philip, but with two additionals. I have had a couple students mix it up without CO2 Freewater only to have them then come and ask me "So is this Lead percipitate?" Yup, seems silly but in these cases they wind up with very rapid percipiation, which I assume was lead carbonate. (If they waited long enough and were careful they could "stain" with the lead free solution and avoid the percipitate . . . but since the lead seems wind up on the bottom and sides of the containers why?)
You can also make CO2 free water with an autoclave. But since most autoclaves either reek or are heated with steam from decades old plumbing lines and reek, I 've never trusted the results to be clean enough to try staining with.
Sounds like an M&M poster to me!
I just overfill the flask so it won't run dry, and I have plenty for the Pb-Cit and the NaOH wash water. Set it to boil, putter around cleaning optics, dishes, dusting, putting away glassware, sorting old samples and doing the 50,000 other little lab tasks until its throughly boiled.
On 10 Oct 2014 at 15:13, jkrupp-at-deltacollege.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Just curious. How essential is it to prepare CO2 free water for mixing } and using lead citrate stain? } } I have always done it, but wonder if anyone has tried skipping it. } } How about alternate ways to prepare it. Boiling is the standard, but } anyone boil using microwave or I have heard sonicating might work too. } } Sitting here with a scalding flask of water, wondering if I can cheat } the EM gods. } } Jon } } Jonathan Krupp } Applied Science, Business & Technology } San Joaquin Delta College } 5151 Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } Find us on Facebook -at- } Electron Microscopy at SJ Delta College } } } } } } } } } } } ==============================Original } Headers============================== 16, 38 -- From } prvs=13532e5fac=jkrupp-at-deltacollege.edu Fri Oct 10 14:45:21 2014 16, } 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu } [207.62.178.237]) 16, 38 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with SMTP id s9AJjKjK020464 16, 38 -- for } {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 14:45:20 -0500 16, 38 -- } Received: from mailin.deltacollege.edu (localhost.localdomain } [127.0.0.1]) 16, 38 -- by localhost (Email Security Appliance) with
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==============================Original Headers============================== 14, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Oct 14 08:21:06 2014 14, 28 -- Received: from znl.com ([206.69.208.20]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9EDL6lr029839 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 14 Oct 2014 08:21:06 -0500 14, 28 -- Received: from localhost (localhost [127.0.0.1]) 14, 28 -- by znl.com (Postfix) with ESMTP id 49F6476E8D6 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 14 Oct 2014 08:21:06 -0500 (CDT) 14, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 28 -- Received: from znl.com ([127.0.0.1]) 14, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 28 -- with ESMTP id p4UEWF1miy3Z for {microscopy-at-microscopy.com} ; 14, 28 -- Tue, 14 Oct 2014 08:21:00 -0500 (CDT) 14, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 14, 28 -- by znl.com (Postfix) with ESMTPA id 5DE8776E8CB 14, 28 -- for {microscopy-at-microscopy.com} ; Tue, 14 Oct 2014 08:21:00 -0500 (CDT) 14, 28 -- Message-ID: {543D233C.8040009-at-microscopy.com} 14, 28 -- Date: Tue, 14 Oct 2014 08:21:00 -0500 14, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 14, 28 -- MIME-Version: 1.0 14, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 28 -- Subject: viaWWW:Ion Beam sputter coater 14, 28 -- References: {201410131516.s9DFGZhA028005-at-ns.microscopy.com} 14, 28 -- In-Reply-To: {201410131516.s9DFGZhA028005-at-ns.microscopy.com} 14, 28 -- X-Forwarded-Message-Id: {201410131516.s9DFGZhA028005-at-ns.microscopy.com} 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
And would add: http://www.ou.edu/research/electron/bmz5364/sato.pdf that lasted without mishap for more than the estimated year at room temp, in the dark in 20ml brown bottles with a PTFE lined screw cap. BUT, in spite of the chemistry, I still boiled the water.
Cheers to all who are also weighed down by the vagaries of toxic lead that causes huge frustrated sighs of toxic gas.
Fred
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmonson-at-wcupa.edu
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Friday, October 10, 2014 5:27 PM To: Monson, Frederick
Jon - What is the downside of doing this just to be sure? There aren't many steps in science easier than "boil water". I have used a microwave but to ensure long enough boiling, it makes it a little messier since the water spills over so a hot plate is probably worth the effort. I am sure sonication works but that is more trouble than needed since you don't have to worry about evaporative loss or flammability when you boil water (unless your lab is adjacent to a fracking well). I have used sonication in the steps involving getting lead nitrate or lead citrate to go into solution but only since I am so compulsive. Could you cheat the gods and skip this step? Sure, 99 times out of a 100. But the downstream problems aren't worth the risk. If you made a batch and got staining artefacts, how would you know if you screwed up the staining step or the error was in not originally boiling the water. I am as frugal and lazy as the next guy but this is as cheap and easy as it gets in sc!
ience. Find something else to eliminate in your life. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Friday, October 10, 2014 2:47 PM To: Phillips, Thomas E.
Just curious. How essential is it to prepare CO2 free water for mixing and using lead citrate stain?
I have always done it, but wonder if anyone has tried skipping it.
How about alternate ways to prepare it. Boiling is the standard, but anyone boil using microwave or I have heard sonicating might work too.
Sitting here with a scalding flask of water, wondering if I can cheat the EM gods.
Jon
Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 16, 38 -- From prvs=13532e5fac=jkrupp-at-deltacollege.edu Fri Oct 10 14:45:21 2014 16, 38 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.237]) 16, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id s9AJjKjK020464 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 14:45:20 -0500 16, 38 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 16, 38 -- by localhost (Email Security Appliance) with SMTP id 5CADDA29291_438374DB 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 19:45:17 +0000 (GMT) 16, 38 -- Received: from zmail.deltacollege.edu (mercury.deltacollege.edu [207.62.178.178]) 16, 38 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 5724A9B04E9_438374DF 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 19:45:17 +0000 (GMT) 16, 38 -- Received: from localhost (localhost [127.0.0.1]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5306DF80DF41 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 12:45:17 -0700 (PDT) 16, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 16, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 16, 38 -- with ESMTP id DilPWkf6EYZx for {Microscopy-at-microscopy.com} ; 16, 38 -- Fri, 10 Oct 2014 12:45:12 -0700 (PDT) 16, 38 -- Received: from localhost (localhost [127.0.0.1]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 00DF1FB285F1 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 12:45:11 -0700 (PDT) 16, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 16, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 16, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 16, 38 -- with ESMTP id X2thFYA8pwa2 for {Microscopy-at-microscopy.com} ; 16, 38 -- Fri, 10 Oct 2014 12:45:11 -0700 (PDT) 16, 38 -- Received: from [10.146.0.11] (unknown [10.146.0.11]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id D8C70F80DF41 16, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 12:45:11 -0700 (PDT) 16, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 16, 38 -- Content-Type: text/plain; charset=us-ascii 16, 38 -- Subject: CO2 free water 16, 38 -- Date: Fri, 10 Oct 2014 12:45:11 -0700 16, 38 -- Message-Id: {B80D321B-694C-4D59-8CBB-A507807107BF-at-deltacollege.edu} 16, 38 -- To: Microscopy-at-microscopy.com 16, 38 -- Mime-Version: 1.0 (Apple Message framework v1085) 16, 38 -- X-Mailer: Apple Mail (2.1085) 16, 38 -- Content-Transfer-Encoding: 8bit 16, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9AJjKjK020464 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 31 -- From PhillipsT-at-missouri.edu Fri Oct 10 16:10:51 2014 26, 31 -- Received: from um-tip1-missouri-out.um.umsystem.edu (um-tip1-missouri-out.um.umsystem.edu [198.209.49.135]) 26, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9ALAoEo009296 26, 31 -- for {microscopy-at-microscopy.com} ; Fri, 10 Oct 2014 16:10:50 -0500 26, 31 -- X-IronPort-Anti-Spam-Filtered: true 26, 31 -- X-IronPort-Anti-Spam-Result: Ah4FAHtKOFTPoJ7S/2dsb2JhbABdAw6DAFNYBMsZCodNAoEFFgF7hAMBAQEEOhQ3AgICAQgRAwEBAQsCEgkHFhsBFAkIAgQBCQkIEQICBIgdDblRhhIBAQEBAQEBAQEBAQEBAQEBAQEBAQEXBI4rgTMQAgEeBhsSBQYLgiVTJIEeBYUVgRiLTINeZIg+PJApg36DN0BsAYFHgQIBAQE 26, 31 -- X-IPAS-Result: Ah4FAHtKOFTPoJ7S/2dsb2JhbABdAw6DAFNYBMsZCodNAoEFFgF7hAMBAQEEOhQ3AgICAQgRAwEBAQsCEgkHFhsBFAkIAgQBCQkIEQICBIgdDblRhhIBAQEBAQEBAQEBAQEBAQEBAQEBAQEXBI4rgTMQAgEeBhsSBQYLgiVTJIEeBYUVgRiLTINeZIg+PJApg36DN0BsAYFHgQIBAQE 26, 31 -- Received: from um-ncas5.um.umsystem.edu ([207.160.158.210]) 26, 31 -- by um-tip1-exch-relay.um.umsystem.edu with ESMTP; 10 Oct 2014 16:10:49 -0500 26, 31 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.90]) by 26, 31 -- UM-NCAS5.um.umsystem.edu ([207.160.158.210]) with mapi id 14.03.0210.002; 26, 31 -- Fri, 10 Oct 2014 16:10:49 -0500 26, 31 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 26, 31 -- To: "jkrupp-at-deltacollege.edu" {jkrupp-at-deltacollege.edu} , 26, 31 -- "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 26, 31 -- Subject: RE: [Microscopy] CO2 free water 26, 31 -- Thread-Topic: [Microscopy] CO2 free water 26, 31 -- Thread-Index: AQHP5ML+cmYAnrx3KEqyhTDeU9/trJwp0fEA 26, 31 -- Date: Fri, 10 Oct 2014 21:10:49 +0000 26, 31 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DDBF9FBDD-at-UM-MBX-T02.um.umsystem.edu} 26, 31 -- References: {201410101947.s9AJlFmx021362-at-ns.microscopy.com} 26, 31 -- In-Reply-To: {201410101947.s9AJlFmx021362-at-ns.microscopy.com} 26, 31 -- Accept-Language: en-US 26, 31 -- Content-Language: en-US 26, 31 -- X-MS-Has-Attach: 26, 31 -- X-MS-TNEF-Correlator: 26, 31 -- x-originating-ip: [128.206.81.115] 26, 31 -- Content-Type: text/plain; charset="us-ascii" 26, 31 -- MIME-Version: 1.0 26, 31 -- Content-Transfer-Encoding: 8bit 26, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9ALAoEo009296 ==============================End of - Headers==============================
==============================Original Headers============================== 38, 40 -- From FMonson-at-wcupa.edu Tue Oct 14 11:17:52 2014 38, 40 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.63.2]) 38, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9EGHqcH023804 38, 40 -- for {microscopy-at-microscopy.com} ; Tue, 14 Oct 2014 11:17:52 -0500 38, 40 -- X-ASG-Debug-ID: 1413303471-062396045501b80001-4CH8be 38, 40 -- Received: from WCUXCHP06.PASSHE.LCL ([144.26.2.142]) by MX01.WCUPA.EDU with ESMTP id 9NtYgQt9zZH4fINL (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO); Tue, 14 Oct 2014 12:17:51 -0400 (EDT) 38, 40 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 38, 40 -- X-Barracuda-Apparent-Source-IP: 144.26.2.142 38, 40 -- X-ASG-Whitelist: Client 38, 40 -- Received: from WCUXCHP08.PASSHE.LCL (144.26.2.144) by WCUXCHP06.PASSHE.LCL 38, 40 -- (144.26.2.142) with Microsoft SMTP Server (TLS) id 15.0.847.32; Tue, 14 Oct 38, 40 -- 2014 12:17:51 -0400 38, 40 -- Received: from WCUXCHP08.PASSHE.LCL ([144.26.2.144]) by WCUXCHP08.PASSHE.LCL 38, 40 -- ([144.26.2.144]) with mapi id 15.00.0847.030; Tue, 14 Oct 2014 12:17:51 -0400 38, 40 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 38, 40 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 38, 40 -- CC: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} 38, 40 -- Subject: RE: [Microscopy] RE: CO2 free water 38, 40 -- Thread-Topic: [Microscopy] RE: CO2 free water 38, 40 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: CO2 free water 38, 40 -- Thread-Index: AQHP5ND0Cz9Wyfe6cUaTb/19fv8UiJwvyIvw 38, 40 -- Date: Tue, 14 Oct 2014 16:17:50 +0000 38, 40 -- Message-ID: {c363f1c91c5a49b694ad7547697debe9-at-WCUXCHP08.PASSHE.LCL} 38, 40 -- References: {201410102127.s9ALRAeZ020186-at-ns.microscopy.com} 38, 40 -- In-Reply-To: {201410102127.s9ALRAeZ020186-at-ns.microscopy.com} 38, 40 -- Accept-Language: en-US 38, 40 -- Content-Language: en-US 38, 40 -- X-MS-Has-Attach: 38, 40 -- X-MS-TNEF-Correlator: 38, 40 -- x-originating-ip: [144.26.2.59] 38, 40 -- Content-Type: text/plain; charset="us-ascii" 38, 40 -- MIME-Version: 1.0 38, 40 -- X-Barracuda-Connect: UNKNOWN[144.26.2.142] 38, 40 -- X-Barracuda-Start-Time: 1413303471 38, 40 -- X-Barracuda-Encrypted: AES256-SHA 38, 40 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 38, 40 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 38, 40 -- X-Barracuda-BRTS-Status: 1 38, 40 -- Content-Transfer-Encoding: 8bit 38, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9EGHqcH023804 ==============================End of - Headers==============================
Dear Listserver, We have a strange phenomenon occurring where RBC are appearing both light and dark in our TEM sections. This occurs with or without staining, In both light and electron microscopy preps. The cells have been plasmodium infected, and processed as follows: 2.5% Glutaraldehyde fixed at least overnight (against our wishes as I recommend a Paraform. Glutaraldehyde mixture for any infected material) 1% Osmicated followed by graded ethanol dehydrations and Epon embedding. Sections are 80 nm thick and normally stained with UA followed by Pb. Any suggestions or references would be highly appreciated.
Sincerely, Michael Delannoy
==============================Original Headers============================== 4, 20 -- From prvs=3578667bd=mdelann1-at-jhmi.edu Tue Oct 14 14:20:36 2014 4, 20 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.200]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9EJKa9n014044 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Oct 2014 14:20:36 -0500 4, 20 -- X-IronPort-AV: E=Sophos;i="5.04,718,1406606400"; 4, 20 -- d="scan'208";a="430467585" 4, 20 -- Received: from bsno8925.ebalto.jhmi.edu (HELO BSNO8925) ([10.16.66.55]) 4, 20 -- by ipex1.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 14 Oct 2014 15:20:36 -0400 4, 20 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 4, 20 -- To: {Microscopy-at-microscopy.com} 4, 20 -- Subject: light vs dark RBC 4, 20 -- Date: Tue, 14 Oct 2014 15:20:33 -0400 4, 20 -- Message-ID: {001a01cfe7e3$ecb0dcc0$c6129640$-at-jhmi.edu} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: text/plain; 4, 20 -- charset="us-ascii" 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- X-Mailer: Microsoft Outlook 14.0 4, 20 -- Thread-Index: Ac/n4rSLKjrneVk5RK6HOAHfU3+UQA== 4, 20 -- Content-Language: en-us ==============================End of - Headers==============================
Good morning (all), Michael, perhaps only talking at large.... this phenomenon perhaps (quite) "normal" due to physiological condition of the RBC'S in a state before, during and after infection with plasmodium(?) "Dark" and "light" morphology of RBC's has been shown by micrographs (description ??) in/by:
Hannes Wickert, Georg Krohne Trends in Parasitology, Volume 23, Issue 10, October 2007, Pages 502-509 The complex morphology of Maurer's clefts: from discovery to three-dimensional reconstructions see: http://www.sciencedirect.com/science/article/pii/S1471492207002218
see: Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome Aitken et al. Malaria Journal 2014, 13:230 Page 5 of 10, Fig.4 http://www.malariajournal.com/content/13/1/230 http://www.malariajournal.com/content/pdf/1475-2875-13-230.pdf
This out of my collected files, it might be there are other reports on ultrastructural morphology on severe search for this phenomenon. Best wishes, good luck, Wolfgang
(Wolfgang MUSS, SALZBURG, AUSTRIA)
} -----Ursprüngliche Nachricht----- } Von: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu] } Gesendet: Dienstag, 14. Oktober 2014 21:25 } An: Muß Wolfgang } Betreff: [Microscopy] Light vs dark RBC [in TEM, plasmodium infected] } ----------------------------------------------------------------------- } --- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Dear Listserver, } } We have a strange phenomenon occurring where RBC are appearing both } light and dark in our TEM sections. } This occurs with or without staining, In both light and electron } microscopy preps. } The cells have been plasmodium infected, and processed as follows: } 2.5% Glutaraldehyde fixed at least overnight (against our wishes as I } recommend a Paraform. Glutaraldehyde mixture for any infected material) } 1% Osmicated followed by graded ethanol dehydrations and Epon } embedding. } Sections are 80 nm thick and normally stained with UA followed by Pb. } Any suggestions or references would be highly appreciated. } } Sincerely, } Michael Delannoy } } } } ==============================Original } Headers============================== } 4, 20 -- From prvs=3578667bd=mdelann1-at-jhmi.edu Tue Oct 14 14:20:36 2014 } 4, 20 -- Received: from smtpauth.johnshopkins.edu } (smtpauth.johnshopkins.edu [162.129.8.200]) } 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id s9EJKa9n014044 } 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Oct 2014 14:20:36 } -0500 } 4, 20 -- X-IronPort-AV: E=Sophos;i="5.04,718,1406606400"; } 4, 20 -- d="scan'208";a="430467585" } 4, 20 -- Received: from bsno8925.ebalto.jhmi.edu (HELO BSNO8925) } ([10.16.66.55]) } 4, 20 -- by ipex1.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 14 Oct } 2014 15:20:36 -0400 } 4, 20 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} } 4, 20 -- To: {Microscopy-at-microscopy.com} } 4, 20 -- Subject: light vs dark RBC } 4, 20 -- Date: Tue, 14 Oct 2014 15:20:33 -0400 } 4, 20 -- Message-ID: {001a01cfe7e3$ecb0dcc0$c6129640$-at-jhmi.edu} } 4, 20 -- MIME-Version: 1.0 } 4, 20 -- Content-Type: text/plain; } 4, 20 -- charset="us-ascii" } 4, 20 -- Content-Transfer-Encoding: 7bit } 4, 20 -- X-Mailer: Microsoft Outlook 14.0 } 4, 20 -- Thread-Index: Ac/n4rSLKjrneVk5RK6HOAHfU3+UQA== } 4, 20 -- Content-Language: en-us } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 41 -- From W.Muss-at-salk.at Wed Oct 15 02:44:23 2014 10, 41 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 10, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9F7iMLj009203 10, 41 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 02:44:22 -0500 10, 41 -- Received: from n1ex214.lks.local (localhost [127.0.0.1]) 10, 41 -- by hermes.salk.at (Postfix) with ESMTP id 83DD3646B90 10, 41 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 09:44:20 +0200 (CEST) 10, 41 -- X-Scanned-By: SALK-Content-Filter 10, 41 -- Received: from hermes.salk.at ([127.0.0.1]) 10, 41 -- by n1ex214.lks.local (n1ex214.lks.local [127.0.0.1]) (amavisd-new, port 10024) 10, 41 -- with ESMTP id dGw10M0aJCdy for {microscopy-at-microscopy.com} ; 10, 41 -- Wed, 15 Oct 2014 09:44:20 +0200 (CEST) 10, 41 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 10, 41 -- by hermes.salk.at (Postfix) with ESMTP id 45073646B8A 10, 41 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 09:44:20 +0200 (CEST) 10, 41 -- Received: from n2dt135.lksdom21.lks.local ([192.168.121.135]) 10, 41 -- by N1EX198.lks.local with ESMTP; 15 Oct 2014 09:54:21 +0200 10, 41 -- Received: from N2DT131.lksdom21.lks.local ([fe80::3826:7715:4ab4:bf3b]) by 10, 41 -- N2DT135.lksdom21.lks.local ([fe80::f805:4bfb:c0ba:912d%19]) with mapi id 10, 41 -- 14.02.0387.000; Wed, 15 Oct 2014 09:44:20 +0200 10, 41 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 10, 41 -- To: "'microscopy-at-microscopy.com LISTSERVER MSA (microscopy-at-microscopy.com)'" 10, 41 -- {microscopy-at-microscopy.com} 10, 41 -- CC: "'mdelann1-at-jhmi.edu'" {mdelann1-at-jhmi.edu} 10, 41 -- Subject: [Microscopy] Re: Light vs dark RBC [plasmodium infected, in TEM] 10, 41 -- Thread-Topic: [Microscopy] Re: Light vs dark RBC [plasmodium infected, in 10, 41 -- TEM] 10, 41 -- Thread-Index: AQHP5+SCtnvFTC+kmEe5VG608pMi+JwwwhMQ 10, 41 -- Date: Wed, 15 Oct 2014 07:44:20 +0000 10, 41 -- Message-ID: {3D22E6EE65F39E448DCE4162815376712D711BD5-at-N2DT131.lksdom21.lks.local} 10, 41 -- References: {201410141924.s9EJOWwO018347-at-ns.microscopy.com} 10, 41 -- In-Reply-To: {201410141924.s9EJOWwO018347-at-ns.microscopy.com} 10, 41 -- Accept-Language: de-DE, en-US 10, 41 -- Content-Language: de-DE 10, 41 -- X-MS-Has-Attach: 10, 41 -- X-MS-TNEF-Correlator: 10, 41 -- x-originating-ip: [192.168.42.2] 10, 41 -- Content-Type: text/plain; charset="iso-8859-1" 10, 41 -- MIME-Version: 1.0 10, 41 -- Content-Transfer-Encoding: 8bit 10, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9F7iMLj009203 ==============================End of - Headers==============================
Postdoctoral Image Analyst in Light Sheet Microscopy Department of Biochemistry, South Parks Road, Oxford Grade 8: ÂŁ38,511 - ÂŁ45,954 p.a.
We are seeking to appoint a Postdoctoral Image Analyst in Light Sheet Microscopy to develop analysis and visualisation tools for data produced by the two light sheet microscopes present within Micron. This position will play a key role in many scientific programmes in collaboration with laboratories associated with Micron Oxford and Nanoscopy Oxford.
Applicants should possess a PhD in biological, physical, engineering sciences, computer sciences or maths and should have extensive experience with computer programming, preferably in Matlab, Python, R and/or Java. Experience in the analysis of microscopy data, together with previous experience in software development, preferably related to image analysis, are essential, as are excellent communication and interpersonal skills. An understanding of both physics and biology, as well as experience in dealing with terabyte scale data sets are also desirable.
This full-time post is funded by the Wellcome Trust for a fixed-term of up to 1 year in the first instance and is based at the Department of Biochemistry, South Parks Road, Oxford.
The actual starting salary offered will be based on qualifications and relevant skills acquired and will also be determined by the funding available. Less experienced candidates may be considered for appointment at Grade 7 (ÂŁ30,434 - ÂŁ37,394 p.a.).
For further general information, phone 01865 613204, quoting reference number 115368.
The closing date for applications is 12.00 midday on Monday 10 November 2014, with interviews for shortlisted candidates on 4/5 December 2014. Contact Person : Margaret Dixon or Rita Emberton Vacancy ID : 115368 Contact Phone : 01865 613204 Closing Date : 10-Nov-2014 Contact Email : jobs-at-bioch.ox.ac.uk
Thanks,
Ian -- Ian Dobbie Micron Bioimaging Unit Manager, Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU Tel: 01865 613323 Email: ian.dobbie-at-bioch.ox.ac.uk
==============================Original Headers============================== 15, 26 -- From ian.dobbie-at-bioch.ox.ac.uk Wed Oct 15 05:14:17 2014 15, 26 -- Received: from relay12.mail.ox.ac.uk (relay12.mail.ox.ac.uk [129.67.1.163]) 15, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9FAEGSV030280 15, 26 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 05:14:17 -0500 15, 26 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205]) 15, 26 -- by relay12.mail.ox.ac.uk with esmtp (Exim 4.80) 15, 26 -- (envelope-from {ian.dobbie-at-bioch.ox.ac.uk} ) 15, 26 -- id 1XeLay-0000v2-dA 15, 26 -- for microscopy-at-microscopy.com; Wed, 15 Oct 2014 11:14:16 +0100 15, 26 -- Received: from bioch2088.bioch.ox.ac.uk ([129.67.72.88] helo=Ians-MacBook-Air-2.local) 15, 26 -- by smtp2.mail.ox.ac.uk with esmtpsa (TLSv1:AES128-SHA:128) 15, 26 -- (Exim 4.69) 15, 26 -- (envelope-from {ian.dobbie-at-bioch.ox.ac.uk} ) 15, 26 -- id 1XeLax-0008LI-8h 15, 26 -- for microscopy-at-microscopy.com; Wed, 15 Oct 2014 11:14:15 +0100 15, 26 -- From: Ian Dobbie {ian.dobbie-at-bioch.ox.ac.uk} 15, 26 -- To: microscopy-at-microscopy.com 15, 26 -- Subject: Postdoctoral Image Analyst in Light Sheet Microscopy 15, 26 -- Date: Wed, 15 Oct 2014 11:14:15 +0100 15, 26 -- Message-ID: {m2y4shbpxk.fsf-at-bioch.ox.ac.uk} 15, 26 -- User-Agent: Gnus/5.13 (Gnus v5.13) Emacs/24.3 (darwin) 15, 26 -- MIME-Version: 1.0 15, 26 -- Content-Type: text/plain; charset=utf-8 15, 26 -- X-Oxford-Username: bioc0882 15, 26 -- Content-Transfer-Encoding: 8bit 15, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9FAEGSV030280 ==============================End of - Headers==============================
Hello Everyone, Size does matter, at least for fonts. I'm running Gatan TEM camera model 780 duel View on my TEM. It's a great improvement from the previous camera, but I would like to increase the font size of the global session information. I can click on each field and change the font size, but doing each of 7 field for each image is too time consuming.
I'm hope one of the Gatan 780 user can advise me on how to globally increase font size.
Thanks!
Frank
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==============================Original Headers============================== 10, 28 -- From frank_karl-at-ardl.com Wed Oct 15 08:57:56 2014 10, 28 -- Received: from cal1-mh678b.smtproutes.com (cal1-mh678b.smtproutes.com [208.70.89.145]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9FDvuft020430 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 08:57:56 -0500 10, 28 -- X-Katharion-ID: 1413381460.76447.cal1-mh678 10, 28 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by 10, 28 -- cal1-mh678.smtproutes.com [(192.69.16.50)] with ESMTP via TCP 10, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 15 Oct 2014 13:57:40 +0000 10, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 10, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Wed, 15 10, 28 -- Oct 2014 09:57:38 -0400 10, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 10, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 10, 28 -- {microscopy-at-microscopy.com} 10, 28 -- Date: Wed, 15 Oct 2014 09:57:38 -0400 10, 28 -- Subject: image font size 10, 28 -- Thread-Topic: image font size 10, 28 -- Thread-Index: Ac/of/sN89y7ec1VRTarFnuYrcnflQ== 10, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C3EC12636-at-exchange2k7.ad.ardl.com} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9FDvuft020430 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Application scientist position
Message: Job Description: CRYO SAMPLE AND SEM SPECIALIST, Koch Institute for Integrative Cancer Research-Nanotechnology Materials Core Facility, to perform cryo sample preparation and EM imaging within the core facility. Will help build out a state-of-the-art EM/cryo-EM suite that has a strong focus on cryo sample preparation, SEM and TEM imaging, AFM imaging, and chemical/material characterization. Responsibilities will include hands-on operation and maintenance of the facilityÂ’s high pressure freezer, freeze fracture, freeze-substitution, cryo microtome, cryo-plunger, and SEM; training facility users; and working closely with researchers at the Koch Institute and greater MIT to develop cryo preservation techniques for a wide variety of novel samples. Will bring creativity and firsthand knowledge to the facility, be comfortable with literature searches and outreach to other experts in the field to troubleshoot new samples as they are developed, and ultimately be integrated into all activities of the EM suite and acquire new skills as the facility brings additional instrumentation online.
Job Requirements: REQUIRED: a bachelorÂ’s degree (masterÂ’s or Ph.D. preferred); at least six years of relevant experience; expertise in cryo sample preparation and SEM imaging; aptitude for learning new skills and teaching others; excellent communication and interpersonal skills; complete mastery of the use of written and spoken English for technical discussion and user training; and ability to interact with a diverse population in a flexible, team-oriented environment. Seek a methodical, detail-oriented individual who is eager to learn and apply new skills. Experience with biological samples is highly desirable. Experience in freeze fracture, TEM, and AFM preferred. http://jobs.mit.edu Job #11043
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Oct 15 16:52:08 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9FLq7Rk030826 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 16:52:07 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id BDD7F76F908 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 16:52:07 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id HgYeyUQSaWl4 for {microscopy-at-microscopy.com} ; 13, 28 -- Wed, 15 Oct 2014 16:52:00 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id E523476F8FB 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 16:52:00 -0500 (CDT) 13, 28 -- Message-ID: {543EEC80.30004-at-microscopy.com} 13, 28 -- Date: Wed, 15 Oct 2014 16:52:00 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Application scientist position CRYO SAMPLE AND SEM SPECIALIST 13, 28 -- References: {201410152035.s9FKZYNI029511-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201410152035.s9FKZYNI029511-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201410152035.s9FKZYNI029511-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 13, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: mckimmye-at-gmail.com Name: Emily McKimmy
Organization: TRW
Title-Subject: [Filtered] ElectroScan 2020
Message: We are decommissioning an ElectroScan 2020 SEM. It has no CPU, no vacuum unit and many boards have been taken. Does anyone have any need for any other parts from it? You pay for shipping and you can have what you need. We are located in Michigan. I have unused 20 um, 30 um, and 50 um apertures. I have a good LaB6 filament. Unrelated but also free I have Tungsten wire baskets, unopened packages of 10. Ted Pella cat# 72-1, 73-1, and 74-1.
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Oct 15 16:53:17 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9FLrHha031047 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 16:53:17 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id A85B776F917 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 16:53:17 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id hqOZycwWXUih for {microscopy-at-microscopy.com} ; 13, 28 -- Wed, 15 Oct 2014 16:53:12 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id AFBA776F90A 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 16:53:12 -0500 (CDT) 13, 28 -- Message-ID: {543EECC8.7070502-at-microscopy.com} 13, 28 -- Date: Wed, 15 Oct 2014 16:53:12 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:ElectroScan 2020 13, 28 -- References: {201410151815.s9FIFbVc001582-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201410151815.s9FIFbVc001582-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201410151815.s9FIFbVc001582-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You may wish to check out some of the DM scripting websites such as Dave Mitchell's site (http://www.dmscripting.com) or the Univ of Graz site (http://portal.tugraz.at/portal/page/portal/felmi/DM-Script). Dave has a script for adding an adjustable scale bar that you may be able to modify for your purpose. (http://www.dmscripting.com/add_adjustable_scale_bar.html) He als has a "scale bar control" which may be useful. (http://www.dmscripting.com/scalebarcontrol.html).
Good luck and let us know if you get a working script!
Cheers, Henk
On 10/15/2014 9:59 AM, frank_karl-at-ardl.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Everyone, } Size does matter, at least for fonts. I'm running Gatan TEM camera model 780 duel View on my TEM. It's a great improvement from the previous camera, but I would like to increase the font size of the global session information. I can click on each field and change the font size, but doing each of 7 field for each image is too time consuming. } } I'm hope one of the Gatan 780 user can advise me on how to globally increase font size. } } Thanks! } } Frank } } } } } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } } ==============================Original Headers============================== } 10, 28 -- From frank_karl-at-ardl.com Wed Oct 15 08:57:56 2014 } 10, 28 -- Received: from cal1-mh678b.smtproutes.com (cal1-mh678b.smtproutes.com [208.70.89.145]) } 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9FDvuft020430 } 10, 28 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2014 08:57:56 -0500 } 10, 28 -- X-Katharion-ID: 1413381460.76447.cal1-mh678 } 10, 28 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by } 10, 28 -- cal1-mh678.smtproutes.com [(192.69.16.50)] with ESMTP via TCP } 10, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 15 Oct 2014 13:57:40 +0000 } 10, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by } 10, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Wed, 15 } 10, 28 -- Oct 2014 09:57:38 -0400 } 10, 28 -- From: Frank Karl {frank_karl-at-ardl.com} } 10, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" } 10, 28 -- {microscopy-at-microscopy.com} } 10, 28 -- Date: Wed, 15 Oct 2014 09:57:38 -0400 } 10, 28 -- Subject: image font size } 10, 28 -- Thread-Topic: image font size } 10, 28 -- Thread-Index: Ac/of/sN89y7ec1VRTarFnuYrcnflQ== } 10, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C3EC12636-at-exchange2k7.ad.ardl.com} } 10, 28 -- Accept-Language: en-US } 10, 28 -- Content-Language: en-US } 10, 28 -- X-MS-Has-Attach: } 10, 28 -- X-MS-TNEF-Correlator: } 10, 28 -- acceptlanguage: en-US } 10, 28 -- Content-Type: text/plain; charset="us-ascii" } 10, 28 -- MIME-Version: 1.0 } 10, 28 -- Content-Transfer-Encoding: 8bit } 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9FDvuft020430 } ==============================End of - Headers==============================
--
Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
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} Below is the result of your form, submitted on Thursday, October 16, 2014 at 12:13:47 AM. } } realname - Zhongyuan Zuo } Email - zhongyuanzuo-at-hotmail.com } ORGANIZATION - Baylor College of Medicine } EDUCATION - Graduate College } LOCATION - Houston } SUBJECT_OF_QUESTION - Ultramicrotome } QUESTION - Hi, } I just join the lab in Baylor as an EM specialist. I am having difficulties with the concentric region finding for Drosophila. I need both Retina and Lamina region from the very centered region in order to get a good section. Any suggestions on alignment of the UC 7 microtome? Also, I have in consistency of the thickness in both inter and intera sections. Please help me. Thank you!
==============================Original Headers============================== 3, 32 -- From oshel1pe-at-cmich.edu Thu Oct 16 07:34:37 2014 3, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9GCYbkk004277 3, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Oct 2014 07:34:37 -0500 3, 32 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 3, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s9GCYa4g008533 3, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Oct 2014 08:34:36 -0400 3, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 3, 32 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 3, 32 -- (TLS) id 14.3.195.1; Thu, 16 Oct 2014 08:34:36 -0400 3, 32 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 3, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 3, 32 -- 14.3.195.1; Thu, 16 Oct 2014 08:34:35 -0400 3, 32 -- Message-ID: {543FBB56.5020409-at-cmich.edu} 3, 32 -- Date: Thu, 16 Oct 2014 08:34:30 -0400 3, 32 -- From: Ask a Microscopist {oshel1pe-at-cmich.edu} 3, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 32 -- MIME-Version: 1.0 3, 32 -- To: micro {Microscopy-at-microscopy.com} 3, 32 -- Subject: Ask-A-Microscopist: Problems thin-sectioning Drosophila 3, 32 -- References: {53842396.30132.1413432834329.JavaMail.EWHSERVER1324$-at-10.10.133.40} 3, 32 -- In-Reply-To: {53842396.30132.1413432834329.JavaMail.EWHSERVER1324$-at-10.10.133.40} 3, 32 -- Content-Type: text/plain; charset="UTF-8"; format=flowed 3, 32 -- Content-Transfer-Encoding: 7bit 3, 32 -- X-Originating-IP: [141.209.160.122] 3, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 32 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 3, 32 -- X-CanItPRO-Stream: default 3, 32 -- X-Canit-Stats-ID: 02N3MyAwd - 96140cf0a919 - 20141016 3, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
We are disposing some equipment that is used with NanoScope II PC's:
CRT VGA monitor for image display
CRT Monochrome monitor for Control display
Interface boards for monochrome monitors
miscellaneous boards for controllers
We are disposing some general purpose video equipment:
-CRT monitors that display NTSC composite video (typically from an optical microscope).
All of this equipment is available for free pickup at our lab in Indianapolis. We need to move it out to make room for more valuable equipment.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: 317-895-5630 Toll free: 800-374-8557 (in USA & Canada) Fax: 317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 15, 36 -- From donc-at-asmicro.com Thu Oct 16 12:40:48 2014 15, 36 -- Received: from resqmta-po-07v.sys.comcast.net (resqmta-po-07v.sys.comcast.net [96.114.154.166]) 15, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9GHemoA001852 15, 36 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2014 12:40:48 -0500 15, 36 -- Received: from resomta-po-06v.sys.comcast.net ([96.114.154.230]) 15, 36 -- by resqmta-po-07v.sys.comcast.net with comcast 15, 36 -- id 3tgM1p0034yXVJQ01tgoE7; Thu, 16 Oct 2014 17:40:48 +0000 15, 36 -- Received: from asm20 ([67.173.27.114]) 15, 36 -- by resomta-po-06v.sys.comcast.net with comcast 15, 36 -- id 3tgn1p0042Tj2Lr01tgnES; Thu, 16 Oct 2014 17:40:47 +0000 15, 36 -- Message-ID: {9CE2A34655B24105B08F4E31C3FD8CF0-at-asm20} 15, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 15, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com} 15, 36 -- Subject: Free NanoScope II and general purpose video equipment 15, 36 -- Date: Thu, 16 Oct 2014 13:40:44 -0400 15, 36 -- MIME-Version: 1.0 15, 36 -- Content-Type: text/plain; 15, 36 -- format=flowed; 15, 36 -- charset="iso-8859-1"; 15, 36 -- reply-type=original 15, 36 -- Content-Transfer-Encoding: 7bit 15, 36 -- X-Priority: 3 15, 36 -- X-MSMail-Priority: Normal 15, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 15, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 15, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 15, 36 -- s=q20140121; t=1413481248; 15, 36 -- bh=ECHMNe+/SGTKl3lSNuPkPMg5us1C3/lQeY+KYiFvUkk=; 15, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version: 15, 36 -- Content-Type; 15, 36 -- b=KQXesSQN/0e7zpN2YIrb5Fx3IKH45DKDFbOIZI5zm7loVZAiw7k2a6Oh6DDXzj+bu 15, 36 -- XpZeXZgIyXNLmk2azLr4gLCQ8Qe79xUhXHRdAPjsTpweJOIE8IMb+WYkGcCAorll0r 15, 36 -- 1/8xVKsLxF1+wotwz9OhdKr/TyisXoB2nsX2xsZcQ6G1GX5femcK8J4Rzmf6WdD3yh 15, 36 -- pZBPJ9jn6yJpSWtN7z9DHtWaRTaLhUl5Yz/Oq8/eOXRohLWoli+SZLVpJup7labGXI 15, 36 -- B3HRclgP+ytWjDMSSgcyi4Q8xtDCNDWb46YRJ2Sk79A/aLv2J+Z7ScpLu5fdvuKs0f 15, 36 -- LJQ4kBuwoEljg== ==============================End of - Headers==============================
We don't have the NanoScope II controller, but we do have STM and AFM equipment that is compatible with NanoScope II. We also sell used or refurbished later model NanoScope controllers, complete systems, etc. regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: 317-895-5630 Toll free: 800-374-8557 (in USA & Canada) Fax: 317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: Justin A. Kraft To: donc-at-asmicro.com Sent: Thursday, October 16, 2014 1:56 PM Subject: [a] Re: [Microscopy] Free NanoScope II and general purpose video equipment
Is the nanoscope available?
} On Oct 16, 2014, at 8:51 PM, donc-at-asmicro.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } } } We are disposing some equipment that is used with NanoScope II PC's: } } CRT VGA monitor for image display } } CRT Monochrome monitor for Control display } } Interface boards for monochrome monitors } } miscellaneous boards for controllers } } We are disposing some general purpose video equipment: } } -CRT monitors that display NTSC composite video (typically from an optical } microscope). } } All of this equipment is available for free pickup at our lab in } Indianapolis. We need to move it out to make room for more valuable } equipment. } } } } regards, } Don } ============================================= } Don Chernoff, Ph.D., President } Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; } Indianapolis IN 46226 USA } E-Mail: donc-at-asmicro.com www.asmicro.com } Voice: 317-895-5630 Toll free: 800-374-8557 (in USA & Canada) } Fax: 317-895-5652 } [business activities since 1990: analytical services in AFM, AFM probes, } consulting, training, calibration and test specimens, } calibration and measurement software, used NanoScope equipment.] } ============================================= } } } ==============================Original Headers============================== } 15, 36 -- From donc-at-asmicro.com Thu Oct 16 12:40:48 2014 } 15, 36 -- Received: from resqmta-po-07v.sys.comcast.net (resqmta-po-07v.sys.comcast.net [96.114.154.166]) } 15, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9GHemoA001852 } 15, 36 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2014 12:40:48 -0500 } 15, 36 -- Received: from resomta-po-06v.sys.comcast.net ([96.114.154.230]) } 15, 36 -- by resqmta-po-07v.sys.comcast.net with comcast } 15, 36 -- id 3tgM1p0034yXVJQ01tgoE7; Thu, 16 Oct 2014 17:40:48 +0000 } 15, 36 -- Received: from asm20 ([67.173.27.114]) } 15, 36 -- by resomta-po-06v.sys.comcast.net with comcast } 15, 36 -- id 3tgn1p0042Tj2Lr01tgnES; Thu, 16 Oct 2014 17:40:47 +0000 } 15, 36 -- Message-ID: {9CE2A34655B24105B08F4E31C3FD8CF0-at-asm20} } 15, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} } 15, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com} } 15, 36 -- Subject: Free NanoScope II and general purpose video equipment } 15, 36 -- Date: Thu, 16 Oct 2014 13:40:44 -0400 } 15, 36 -- MIME-Version: 1.0 } 15, 36 -- Content-Type: text/plain; } 15, 36 -- format=flowed; } 15, 36 -- charset="iso-8859-1"; } 15, 36 -- reply-type=original } 15, 36 -- Content-Transfer-Encoding: 7bit } 15, 36 -- X-Priority: 3 } 15, 36 -- X-MSMail-Priority: Normal } 15, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 } 15, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 } 15, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; } 15, 36 -- s=q20140121; t=1413481248; } 15, 36 -- bh=ECHMNe+/SGTKl3lSNuPkPMg5us1C3/lQeY+KYiFvUkk=; } 15, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version: } 15, 36 -- Content-Type; } 15, 36 -- b=KQXesSQN/0e7zpN2YIrb5Fx3IKH45DKDFbOIZI5zm7loVZAiw7k2a6Oh6DDXzj+bu } 15, 36 -- XpZeXZgIyXNLmk2azLr4gLCQ8Qe79xUhXHRdAPjsTpweJOIE8IMb+WYkGcCAorll0r } 15, 36 -- 1/8xVKsLxF1+wotwz9OhdKr/TyisXoB2nsX2xsZcQ6G1GX5femcK8J4Rzmf6WdD3yh } 15, 36 -- pZBPJ9jn6yJpSWtN7z9DHtWaRTaLhUl5Yz/Oq8/eOXRohLWoli+SZLVpJup7labGXI } 15, 36 -- B3HRclgP+ytWjDMSSgcyi4Q8xtDCNDWb46YRJ2Sk79A/aLv2J+Z7ScpLu5fdvuKs0f } 15, 36 -- LJQ4kBuwoEljg== } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 38 -- From donc-at-asmicro.com Thu Oct 16 13:50:51 2014 8, 38 -- Received: from resqmta-po-11v.sys.comcast.net (resqmta-po-11v.sys.comcast.net [96.114.154.170]) 8, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9GIooHH022633 8, 38 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2014 13:50:50 -0500 8, 38 -- Received: from resomta-po-12v.sys.comcast.net ([96.114.154.236]) 8, 38 -- by resqmta-po-11v.sys.comcast.net with comcast 8, 38 -- id 3upy1p00656HXL001uqqXe; Thu, 16 Oct 2014 18:50:50 +0000 8, 38 -- Received: from asm20 ([67.173.27.114]) 8, 38 -- by resomta-po-12v.sys.comcast.net with comcast 8, 38 -- id 3uqp1p00V2Tj2Lr01uqqmL; Thu, 16 Oct 2014 18:50:50 +0000 8, 38 -- Message-ID: {CECC105514BC48DAA31A770492758A5A-at-asm20} 8, 38 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 8, 38 -- To: "Justin A. Kraft" {kraftpiano-at-gmail.com} 8, 38 -- Cc: "Microscopy List" {microscopy-at-microscopy.com} 8, 38 -- References: {201410161751.s9GHpAfk008727-at-ns.microscopy.com} {85D93E27-17AA-46A7-98E7-8BCCBDBAD0E7-at-gmail.com} 8, 38 -- Subject: Re: [a] Re: [Microscopy] Free NanoScope II and general purpose video equipment 8, 38 -- Date: Thu, 16 Oct 2014 14:51:01 -0400 8, 38 -- MIME-Version: 1.0 8, 38 -- Content-Type: text/plain; 8, 38 -- format=flowed; 8, 38 -- charset="iso-8859-1"; 8, 38 -- reply-type=original 8, 38 -- Content-Transfer-Encoding: 7bit 8, 38 -- X-Priority: 3 8, 38 -- X-MSMail-Priority: Normal 8, 38 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 8, 38 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 8, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 8, 38 -- s=q20140121; t=1413485450; 8, 38 -- bh=CDslqUPYzG0jBSK+NcbRe3LljL58pakHQnw1/EHeP8E=; 8, 38 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version: 8, 38 -- Content-Type; 8, 38 -- b=YpgbThejUdddNouV1VV+9XOaWl5HBWfZ3MLFqFcE6p3H/6aZsAbVLPh5V/pmeOmj8 8, 38 -- eMicxFoW00/acNCvBiFs2S896eiojGLpYWYUcLrmiULyXpKRabS1emT3VPGNLT3vZD 8, 38 -- Lf7m0VLIy8Ag8J+CZSteS5sAQEz7670CN+HEmS8yr0vH/T3yYQ26EhquB1dyo6A58s 8, 38 -- Pc+rTmoQypKLbXI5aRmGzkfTwZzxqp2GGmYhKxEHzbOshSs9f3mgHmyIjuCRcX8heV 8, 38 -- f6JJobDxlFHGBSDPp3MVuX8rfVgNBVZgFTZcE+di9EOoSY5m8CHNg+CjMsQVfxoGEY 8, 38 -- A0W2gcbmReFwA== ==============================End of - Headers==============================
I have not done any of this recently, and is therefore not fresh in my mind, but I remember that good fixation, embedding and therefore sectioning of arthropod eyes can be challenging. Samples with highly variable internal density always seem to be tricky. The exoskeleton can form an excellent barrier to ingress of chemicals (at least in the much larger spiders that I dealt with). I suspect the key will be in the sample preparation rather than the settings on the microtome.
For sample prep, partial dissection, punching little holes etc. all helps. I have talked to people (admittedly dealing with even larger more armoured arthropods) that use DMSO in some of their solutions.
In order to get the best help from people on this forum, you may have to describe the problem you are having in more detail and keep in mind that not everyone, including I, will understand what "the concentric region" is exactly.
Kind regards Duane
AgResearch Limited | Lincoln Research Centre | New Zealand Dr Duane P Harland, Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Friday, 17 October 2014 1:58 a.m. To: Harland, Duane
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} Below is the result of your form, submitted on Thursday, October 16, 2014 at 12:13:47 AM. } } realname - Zhongyuan Zuo } Email - zhongyuanzuo-at-hotmail.com } ORGANIZATION - Baylor College of Medicine EDUCATION - Graduate College } LOCATION - Houston SUBJECT_OF_QUESTION - Ultramicrotome QUESTION - Hi, } I just join the lab in Baylor as an EM specialist. I am having difficulties with the concentric region finding for Drosophila. I need both Retina and Lamina region from the very centered region in order to get a good section. Any suggestions on alignment of the UC 7 microtome? Also, I have in consistency of the thickness in both inter and intera sections. Please help me. Thank you!
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Digital counter for filament usage
Message: Hi All, Is there any time counter for filament on/off available with FEI or third party?
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Message: If you are a EELS or EFTEM user, you may be interested in attending this 30 minute webinar. It will be given at 8:00-8:30 am PDT USA and also at 4:00-4:30 am PDT USA on Monday, October 27, 2014.
STEM EELS spectrum imaging can reveal composition and chemical changes at the nano-scale and even the atomic scale in many cases. To reveal this information, the researcher needs to optimize not only the spectrometer, but also the sample and the STEM configuration. In this webinar, we will discuss how to optimize the configuration of the STEM and EELS system for high-speed spectrum imaging analysis. We will review the requirements of EELS camera and spectrometer settings needed to push the limits of spectrum image performance.
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I assume your FEI scope has a computer interface - I keep track of filament use with a Macro Express macro hooked to a command icon (also through macro express) for our JEOL 5600. When I click the icon, the macro starts/stops the microscope high tension and writes a timestamp to a text file, calculating elapsed session time and total beam time when the HT is turned off. Fairly simple, really. I can send you details if you're interested.
Macro Express isn't free, but well worth the trivial amount it costs. It automates all kinds of things I do routinely on scope computers and elsewhere. See http://www.macros.com/
No commercial interest, just a happy customer.
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
For Sale: Parachute. Only used once, never opened, small stain.
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==============================Original Headers============================== 17, 20 -- From jehrman-at-mta.ca Fri Oct 17 08:15:24 2014 17, 20 -- Received: from smtpy.mta.ca (smtpy.mta.ca [138.73.1.205]) 17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9HDFNw4018317 17, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Oct 2014 08:15:24 -0500 17, 20 -- Received: from host-22-169.mta.ca ([138.73.22.169]:54563) 17, 20 -- by smtpy.mta.ca with esmtpsa (TLSv1:DHE-RSA-AES128-SHA:128) 17, 20 -- (Exim 4.84) 17, 20 -- (envelope-from {jehrman-at-mta.ca} ) 17, 20 -- id 1Xf7NK-0004De-4Q; Fri, 17 Oct 2014 10:15:22 -0300 17, 20 -- Message-ID: {5441168C.8030806-at-mta.ca} 17, 20 -- Date: Fri, 17 Oct 2014 10:15:56 -0300 17, 20 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 17, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 17, 20 -- MIME-Version: 1.0 17, 20 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} , ravi.thakkar369-at-gmail.com 17, 20 -- Subject: Re: [Microscopy] viaWWW:Digital counter for filament usage 17, 20 -- References: {201410171307.s9HD7gos011553-at-ns.microscopy.com} 17, 20 -- In-Reply-To: {201410171307.s9HD7gos011553-at-ns.microscopy.com} 17, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
When the HT is turned on, the 12 volt filament supply is powered up. If you take a 12 volt timer and tie it to the power supply, it will give you the HT ON time. Since we turn the HT off between users, it served us well as a usage timer.
Henk
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Hendrik O. Colijn
*C*enter for *E*lectron *M*icroscopy & *A*nalysi*S*
Why do you ask? Do you have a W gun or a field emission gun?
If you are seeking to track filament lifetime, that can probably be done rather easily if you can find the right connection to a circuit showing when the high voltage is on. I don't know why you would want to do that with a field emission gun.
If you are seeking to track hours for billing I can understand that and there are multiple ways to do it. - You might be able to tie into a circuit that show voltage when the high voltage is on. There are hour meters available through McMaster Carr and other outlets than run when a voltage is applied to them. - We have considered having a program run in front of the user interface so that users need to log in to clear it and access the UI. That could give us a lot more information. - We have also heard of someone putting a box inline with the monitor power. The monitor can only be energized when someone logs into another monitor computer.
Warren ________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com {microscopylistserver-noreply-at-microscopy.com} Sent: Friday, October 17, 2014 8:08 AM To: Straszheim, Warren E [BIOTC]
X-from: ravi.thakkar369-at-gmail.com ()
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Digital counter for filament usage
Message: Hi All, Is there any time counter for filament on/off available with FEI or third party?
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Online registration and abstract submission are now open for AMAS XIII, the 13th biennial Australian Microbeam Analysis Symposium, which will be held in Hobart, Tasmania, on 11-13 February 2015, with Pre-Meeting Workshops 9-10 Feb, 2015.
The AMAS symposia provide a forum to discuss and share ideas on advances, trends and challenges in microanalysis and imaging with national and international leaders in the field, with an emphasis on practical solutions and applications.
Please see our website
http://www.microscopy.org.au/amas/amas13/
for more information such as second circular, invited speakers, workshop program, registration, accommodation, and student travel bursary information.
Please feel free to forward this email to your colleagues and anyone that you think might be interested in attending.
Many thanks,
Karsten
AMAS XIII Co-Chair
Dr Karsten Goemann Research Fellow, Electron Microscopy & X-Ray Microanalysis Central Science Laboratory, University of Tasmania Mail: Private Bag 74, Hobart TAS 7001, Australia Location: Rooms 254-256, Chemistry Building, Dobson Road, Sandy Bay TAS 7005, Australia T: +61 (0)3 6226 2146 | F: +61 (0)3 6226 2494 | M: +61 (0)407 101 990 www.utas.edu.au/research/central-science-laboratory CRICOS 00586B
==============================Original Headers============================== 13, 37 -- From Karsten.Goemann-at-utas.edu.au Sun Oct 19 17:09:25 2014 13, 37 -- Received: from mx1.utas.edu.au (ironport2.its.utas.edu.au [131.217.6.12]) 13, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9JM9Nrs024268 13, 37 -- for {Microscopy-at-microscopy.com} ; Sun, 19 Oct 2014 17:09:24 -0500 13, 37 -- X-IronPort-AV: E=Sophos;i="5.04,749,1406556000"; 13, 37 -- d="scan'208";a="126893717" 13, 37 -- Received: from unknown (HELO mmp-sby1.its.utas.edu.au) ([10.10.3.71]) 13, 37 -- by ironport2-outgoing.utas.edu.au with ESMTP; 20 Oct 2014 09:09:21 +1100 13, 37 -- MIME-version: 1.0 13, 37 -- Content-transfer-encoding: 7BIT 13, 37 -- Content-type: text/plain; CHARSET=US-ASCII 13, 37 -- Received: from MBX2.utas.ad.internal ([10.10.5.239]) 13, 37 -- by mmp-sby1.its.utas.edu.au 13, 37 -- (Sun Java(tm) System Messaging Server 7u3-17.01 64bit (built Jun 8 2010)) 13, 37 -- with ESMTP id {0NDP00ILXPJLG8B0-at-mmp-sby1.its.utas.edu.au} for 13, 37 -- Microscopy-at-Microscopy.com; Mon, 20 Oct 2014 09:09:21 +1100 (EST) 13, 37 -- Received: from MBX11.utas.ad.internal (10.10.5.230) 13, 37 -- by MBX2.utas.ad.internal (10.10.5.239) with Microsoft SMTP Server (TLS) 13, 37 -- id 15.0.995.29; Mon, 20 Oct 2014 09:09:20 +1100 13, 37 -- Received: from MBX11.utas.ad.internal ([fe80::bd85:84c8:fac:5c74]) 13, 37 -- by MBX11.utas.ad.internal ([fe80::bd85:84c8:fac:5c74%17]) 13, 37 -- with mapi id 15.00.0995.028; Mon, 20 Oct 2014 09:09:20 +1100 13, 37 -- From: Karsten Goemann {Karsten.Goemann-at-utas.edu.au} 13, 37 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 13, 37 -- Cc: Leonid Danyushevsky {L.Dan-at-utas.edu.au} , 13, 37 -- Jay Thompson {Jay.Thompson-at-utas.edu.au} 13, 37 -- Subject: AMAS XIII registration now open 13, 37 -- Thread-topic: AMAS XIII registration now open 13, 37 -- Thread-index: AQHP6+lVN8tLeq3v4E6zQU1EtgbC9g== 13, 37 -- Date: Sun, 19 Oct 2014 22:09:20 +0000 13, 37 -- Message-id: {DCBF8EDE-44B3-4393-816A-3A9E711F8356-at-utas.edu.au} 13, 37 -- Accept-Language: en-AU, en-US 13, 37 -- Content-language: en-US 13, 37 -- X-MS-Has-Attach: 13, 37 -- X-MS-TNEF-Correlator: 13, 37 -- X-Originating-IP: [131.217.50.107] 13, 37 -- Content-id: {03E77DD48FDB6342A5DB6097FB0BB6FA-at-postoffice.utas.edu.au} ==============================End of - Headers==============================
Recently one of our users asked about how to prepare synthetic scaffolds with cells attached. The material make the scaffolds dissolves in ethanol. So is there any way to avoid using ethanol but using something else (e.g. Acetone) and proceed with critical point drying? Or we might have to do freeze drying?
Any advice will be appreciated!
Xuanhao Sun, Ph.D.
Bioscience Electron Microscopy Laboratory University of Connecticut BPB G06, Unit 3242 Storrs, CT 06269-3242
Recently one of our users asked about how to prepare synthetic scaffolds with cells attached. The material used to make the scaffolds dissolves in ethanol. So is there any way to avoid using ethanol but using something else (e.g. Acetone) and proceed with critical point drying? Or we might have to do freeze drying?
Any advice will be appreciated!
Xuanhao Sun, Ph.D. Bioscience Electron Microscopy Laboratory University of Connecticut BPB G06, Unit 3242 Storrs, CT 06269-3242 Office Tel: 860-486-6368 Lab Tel: 860-486-2914 Fax: 860-486-6369 website: emlab.uconn.edu =======================
Dear Xuanhao,
Yes, you may critical point dry in Acetone but before you do I'd ask the investigators if they have tried acetone on the scaffolds for it may dissolve them too. Another question would be have they fixed the material with glutaraldehyde followed by osmium tetroxide before putting it into ethanol? If it becomes stable it could eliminate your problem.
The second point is that the vapors from the CPD need to be vented into a chemical hood so that you are not exposed to the acetone which one should not breathe. Run off a copy of the MSDS sheet for safety recommendations.
The third point is that you will need "dry acetone". Do not use acetone from a big old jug! I have successfully used Mallinckrodt 2440 Analytical reagent grade. It has a water content of 0.2%. It is available in pint bottles that are good for they do not set around for a long time before being used up like the gallons do. I had made the dilutions from it and of course used it in the CPD chamber at 100%. I believe that several of the EM suppliers also supply acetone that is "dry".
I have heard of using amyl acetate in the CPD but know that the old Sorval CPD instructions said that the O-rings needed to be changed to a different material for the standard ones would not hold up. Amyl acetate is also nasty and must be vented to a hood. I do not recall what was used for the dehydration before putting the samples into amyl acetate for I had not used it.
HMDS is sometimes used in place of CPD but ethanol is used for dehydration up to 100% so that is not a good substitute.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Pat
Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491 connellyps-at-mail.nih.gov
I had a question for a local histopathologist that I decided to extend to the List:
I have a client who is looking at deep-sea, meso- and surface soft coral polyps, Gorgonians. She has contributed thousands of samples to (redacteD) Museum, and they are having a strong disagreement about how they should be stored. The old-timers say to store in 70% ethanol. I have more recently heard arguments for 85% ethanol, but I can't document that. And she is determined to store them in 95% ethanol, which sme museums seem to be adopting. Certainly the protein and genomics look a lot better in meterial stored in the 95% ethanol. Her mentor recently looked at polyps stored in 95% in one of those desktop SEMs, and he says he has less shriveling, but we don't really have a good experimental set. While we wait to design experiments to test this, I thought I would ask your opinion. What is the current word on long-term storage for histopathology/museum specimens?
Meanwhile, she also had to collect some specimens into different concentrations of rum, and now I want to test that, too, since it will also have some sugars to keep the osmolarity up...!
Thoughts?
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
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We're about to move into a temporary facility, and before doing so had the SEM vendor check the site to see if the vibration and electromagnetic interference was within spec. The site has excess vibration from 0.1-3 Hz, although it's not much over spec, but it was well out of spec for EMI. The vendor recommended installing an active field cancelling system.
So, the architects got their own electromagnetic inspection and lo and behold, they said the site was OK, so we're not getting a field cancelling system. We're definitely having an inspection before re-installation of the instrument, but I'm a bit stymied as to what we'll do if it's very much out of spec.
Has this happened to anyone and how did you handle it?
thanks, Rosemary
p.s. this email was rejected at first when in html format, not sure why.
Dr Rosemary White CSIRO Black Mountain GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
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I expect to be in the same boat in a couple of years. Architects don't like to listen to people who will be using their building. I suggest pointing out to the money people that the EM vendors know their instrument requirements and how to measure for them better than anyone else. From experience. If the building doesn't meet their criteria, then all performance warranties are void. If the instruments can't work properly because of the building, then they are just expensive paperweights. Fixing the problem after the fact is *much* more expensive than doing the job right in the first place. You might also arrange a meeting between the vendor people and the architect people to hash out the conflict. Both should want things to work right.
Phil
} Dear all, } } We're about to move into a temporary facility, and before doing so had the } SEM vendor check the site to see if the vibration and electromagnetic } interference was within spec. The site has excess vibration from 0.1-3 Hz, } although it's not much over spec, but it was well out of spec for EMI. The } vendor recommended installing an active field cancelling system. } } So, the architects got their own electromagnetic inspection and lo and } behold, they said the site was OK, so we're not getting a field cancelling } system. We're definitely having an inspection before re-installation of } the instrument, but I'm a bit stymied as to what we'll do if it's very } much out of spec. } } Has this happened to anyone and how did you handle it? } } thanks, } Rosemary } } p.s. this email was rejected at first when in html format, not sure why. } } Dr Rosemary White } CSIRO Black Mountain } GPO Box 1600 } Canberra, ACT 2601 } Australia } } T 61 2 6246 5475 } F 61 2 6246 5334 } E rosemary.white-at-csiro.au -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 32 -- From oshel1pe-at-cmich.edu Wed Oct 22 06:18:22 2014 4, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MBIMkN005991 4, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 06:18:22 -0500 4, 32 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 4, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s9MBIJeA022326 4, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 07:18:20 -0400 4, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 4, 32 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 4, 32 -- (TLS) id 14.3.195.1; Wed, 22 Oct 2014 07:18:19 -0400 4, 32 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 4, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 32 -- 14.3.195.1; Wed, 22 Oct 2014 07:18:19 -0400 4, 32 -- Message-ID: {5447927B.6080306-at-cmich.edu} 4, 32 -- Date: Wed, 22 Oct 2014 07:18:19 -0400 4, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 32 -- MIME-Version: 1.0 4, 32 -- To: micro {Microscopy-at-microscopy.com} 4, 32 -- Subject: Re: [Microscopy] site inspections - overriding vendor recommendations 4, 32 -- References: {201410220134.s9M1YjUJ020300-at-ns.microscopy.com} 4, 32 -- In-Reply-To: {201410220134.s9M1YjUJ020300-at-ns.microscopy.com} 4, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 32 -- Content-Transfer-Encoding: 7bit 4, 32 -- X-Originating-IP: [141.209.160.122] 4, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 32 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 4, 32 -- X-CanItPRO-Stream: default 4, 32 -- X-Canit-Stats-ID: 02N6bijMp - 2144e16c09c1 - 20141022 4, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Hello, The electromagnetic interferences can change in magnitude! You have to carefully examine from which source the fields are produced. If they are, for instance, generated in an electric cable passing close to you SEM, and taking into account that the field strength depends on the current loaded on that cable, then you can easily imagine that if that cable serves in your building to power the air conditioning system, then you will observe very heavy and changing field strength over time. You will understand that the field might at certain times of the day be low, but at other times of the day produce unacceptable interferences. Something like this could explain why two professionals measured different field strength in your area. We, encountering a similar situation some years ago, let a professional inspection of the electrical system in our building be done and found several violations of good practice of how to install power cables in a building. Correcting these reduced the electromagnetic interferences in the electron microscopy laboratories significantly - and we really have suffered very strong interferences! While the electron microscopy vendor did an excellent job in detecting the presence of the fields, and the vendor, as well as some other companies, offered some nice solutions (active field cancellation systems, passive shielding), none of them had a solution for in detail locating the source of the problem and a solution to just remove that cause of the problem. As certified electricians are usually also not educated to conclude from a present field to the source of the problem, we have got the electrical system check in the building be done by a real professional on electromagnetic interferences, and the costs for his work and the corrections in the cabling (needed to be done some 20 meters away from the laboratory!) reduced the final total costs for the solution remarkably.
I that time have been in contact with many (internationally operating) shielding solution companies, from various continents, but in fact only one did care for the mayor cause of the problem and first solved that one before offering a final (then much smaller) shielding solution. Although I am just a perfectly happy customer, I guess that I am not supposed to publish company names here on the microscopy-list, so contact me for further information if needed.
Wish you all the best, with best greetings from the EM-Labs of CIC biomaGUNE, Marco Möller
---------------------------------------------------------------- Marco Möller Platform Manager - Electron Microscopy
CIC biomaGUNE Parque Tecnológico de San Sebastián Paseo Miramón, 182. Ed. Empresarial C 20009 San Sebastián (Guipúzcoa) SPAIN
Tel: +34 943 00 53 25 mmoller-at-cicbiomagune.es
-----Original Message----- X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au] Sent: Wednesday, October 22, 2014 3:33 AM To: Marco Moller
Dear all,
We're about to move into a temporary facility, and before doing so had the SEM vendor check the site to see if the vibration and electromagnetic interference was within spec. The site has excess vibration from 0.1-3 Hz, although it's not much over spec, but it was well out of spec for EMI. The vendor recommended installing an active field cancelling system.
So, the architects got their own electromagnetic inspection and lo and behold, they said the site was OK, so we're not getting a field cancelling system. We're definitely having an inspection before re-installation of the instrument, but I'm a bit stymied as to what we'll do if it's very much out of spec.
Has this happened to anyone and how did you handle it?
thanks, Rosemary
p.s. this email was rejected at first when in html format, not sure why.
Dr Rosemary White CSIRO Black Mountain GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
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==============================Original Headers============================== 29, 36 -- From mmoller-at-cicbiomagune.es Wed Oct 22 08:00:27 2014 29, 36 -- Received: from mail.cicbiomagune.es (mail.cicbiomagune.es [150.241.252.35]) 29, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MD0Q3G028693 29, 36 -- for {microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 08:00:26 -0500 29, 36 -- X-AuditID: 0a0d0165-f797b6d000001031-10-5447aa694f31 29, 36 -- Received: from CICMGE007.cicbiomagune.int (cicmge008.cicbiomagune.int [10.13.1.108]) 29, 36 -- by mail.cicbiomagune.es (Symantec Messaging Gateway) with SMTP id 9A.4D.04145.96AA7445; Wed, 22 Oct 2014 15:00:25 +0200 (CEST) 29, 36 -- Content-Class: urn:content-classes:message 29, 36 -- MIME-Version: 1.0 29, 36 -- Content-Type: text/plain; 29, 36 -- charset="iso-8859-1" 29, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4913 29, 36 -- Subject: RE: site inspections - overriding vendor recommendations 29, 36 -- Date: Wed, 22 Oct 2014 15:00:24 +0200 29, 36 -- Message-ID: {0076FB6F2203944CAAA265CF412935E8D025C7-at-CICMGE007.cicbiomagune.int} 29, 36 -- In-Reply-To: {201410220132.s9M1WuEe017751-at-ns.microscopy.com} 29, 36 -- X-MS-Has-Attach: 29, 36 -- X-MS-TNEF-Correlator: 29, 36 -- Thread-Topic: site inspections - overriding vendor recommendations 29, 36 -- thread-index: Ac/tmEHBmk3LMwxdT2qr2rTbh37WMgAWfjLA 29, 36 -- References: {201410220132.s9M1WuEe017751-at-ns.microscopy.com} 29, 36 -- From: "Marco Moller" {mmoller-at-cicbiomagune.es} 29, 36 -- To: {microscopy-at-microscopy.com} 29, 36 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFnrBLMWRmVeSWpSXmKPExsXCxcuYo5u5yj3EYMkPTYs/hxvYLU482cHu 29, 36 -- wORx68BTVo+P3a+ZApiiuGxSUnMyy1KL9O0SuDJ6dl9gK7juVLH5RCd7A+Mf8y5GTg4JAROJ 29, 36 -- 9btnsEPYYhIX7q1n62Lk4hASuMoosfTPY0aQBLOAlsSOCfvAbF4BQYmTM5+wQMQNJO4f6mCF 29, 36 -- sLUlli18zdzFyAE0yELi+o8EkLCwgKPE/VmdYPNZBFQlpl+5DTXGX+Lh/8dgYzgFbCQur/7J 29, 36 -- AnGDoMSi2XuYYe75t+shG4gtIuAkcevRPmYI20ji15UXTCC2kIC1xIMHs8FsNgE9iWvTr7OA 29, 36 -- nCAiIC/xtSFxAqPwLCQPzELywCwkD8xC8sACRpZVjNJJRZnpGSW5iZk5esmZyUmZ+bmJ6aV5 29, 36 -- qXrAONjECAn/1B2M+yfqHmIU4GBU4uGdweEeIsSaWFZcmXuIUYKDWUmEV6keKMSbklhZlVqU 29, 36 -- H19UmpNafIhRmoNFSZz3C5tmiJBAemJJanZqakFqEUyWiYNTqoFRrSv8046tM5hK2bh/Vl8U 29, 36 -- MrjgVSMeOCMkODEl98BX9YMTLzYdCueXTmaZek7oZNvv1kmKxkvzOk/Ga3f1x7HMnm31u8L1 29, 36 -- dinPt/MhljV39IXmXdjYH+LkXlL8w1PkwyTtby5KrY83/PQW3W4bq/Tp0Mo4X98Wy82HcnMD 29, 36 -- JboPyPn8mCwd1KjEUpyRaKjFXFScCAC+svCEewIAAA== 29, 36 -- Content-Transfer-Encoding: 8bit 29, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9MD0Q3G028693 ==============================End of - Headers==============================
For long-term storage ... more than one preservative. Some samples in 95% for future protein/genomics work, some in 70% EtOH (maybe 80%) + 5% glycerin for external morphology.
To be really exciting, I'd also process some samples for light or confocal microscopy/TEM/SEM - fix/dehydrate/embed, and store as embedded samples. But long-term storage in alchohols is likely to lead to extracting lipids and the morphology will Go Away. Oh! Is there any possibility that the museum can store the samples below 25 deg C? 68 F. If yes, get them through 95% EtOH, then into 100% t-butanol, let that freeze (25 C), and store them in that. Be better than in EtOH of any %. I assume t-BuOH is good for genomics, etc., but that should be checked, and the morphology should be better. Decent for SEM, anyway.
Tissues in fixative are fine after a couple of years (even for TEM), but ... archival? Do you or the museum have some old samples that can be sectioned and examined?
Phil
} I had a question for a local histopathologist that I decided to extend to } the List: } } I have a client who is looking at deep-sea, meso- and surface soft coral } polyps, Gorgonians. She has contributed thousands of samples to (redacteD) } Museum, and they are having a strong disagreement about how they should be } stored. The old-timers say to store in 70% ethanol. I have more recently } heard arguments for 85% ethanol, but I can't document that. And she is } determined to store them in 95% ethanol, which sme museums seem to be } adopting. Certainly the protein and genomics look a lot better in meterial } stored in the 95% ethanol. Her mentor recently looked at polyps stored in } 95% in one of those desktop SEMs, and he says he has less shriveling, but } we don't really have a good experimental set. While we wait to design } experiments to test this, I thought I would ask your opinion. What is the } current word on long-term storage for histopathology/museum specimens? } } Meanwhile, she also had to collect some specimens into different } concentrations of rum, and now I want to test that, too, since it will } also have some sugars to keep the osmolarity up...! } } Thoughts? } } Aloha, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 35 -- From oshel1pe-at-cmich.edu Wed Oct 22 09:44:19 2014 6, 35 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MEiIBj019842 6, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 09:44:18 -0500 6, 35 -- Received: from CAS3.central.cmich.local ([141.209.15.90]) 6, 35 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s9MEiCGg005057 6, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 10:44:14 -0400 6, 35 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 6, 35 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 6, 35 -- (TLS) id 14.3.195.1; Wed, 22 Oct 2014 10:44:17 -0400 6, 35 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 6, 35 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 6, 35 -- (TLS) id 14.3.195.1; Wed, 22 Oct 2014 10:44:16 -0400 6, 35 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 6, 35 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 6, 35 -- 14.3.195.1; Wed, 22 Oct 2014 10:44:16 -0400 6, 35 -- Message-ID: {5447C2C0.9020504-at-cmich.edu} 6, 35 -- Date: Wed, 22 Oct 2014 10:44:16 -0400 6, 35 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 35 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 35 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 35 -- MIME-Version: 1.0 6, 35 -- To: micro {Microscopy-at-microscopy.com} 6, 35 -- Subject: Re: [Microscopy] Long-term ethanol storage 6, 35 -- References: {201410212128.s9LLSmYq025446-at-ns.microscopy.com} 6, 35 -- In-Reply-To: {201410212128.s9LLSmYq025446-at-ns.microscopy.com} 6, 35 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 35 -- Content-Transfer-Encoding: 7bit 6, 35 -- X-Originating-IP: [141.209.160.122] 6, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 35 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 6, 35 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 6, 35 -- X-CanItPRO-Stream: default 6, 35 -- X-Canit-Stats-ID: 05N6eIeUf - 3b10bcefc2af - 20141022 6, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Dear All, I would like to hear from you advices if one will prefably choose a TMP unit over a diffusion pump for the camera chamber. So far, i learn that TMP will take a shorter time to pump down the chamber. May i ask if there are many other advantages of using TMP over the diffusion pump?
Will it also reduce carbon contamination on the sample even though the sample should be in sample column area which is generally pumped down by an SIP.
If the turbo is backed by a scroll pump (dry): less hydrocarbon contamination, it is also better for any (cooled) cameras, you will need less heating cycles to keep the camera clean. In my experience any dry pump system will require less maintenance both on service and user level.
Best,
Wim Hagen EMBL Heidelberg
} On Oct 22, 2014, at 18:42, rongchigram79-at-yahoo.com.sg wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, I would like to hear from you advices if one will prefably choose a TMP unit over a diffusion pump for the camera chamber. So far, i learn that TMP will take a shorter time to pump down the chamber. May i ask if there are many other advantages of using TMP over the diffusion pump? } } Will it also reduce carbon contamination on the sample even though the sample should be in sample column area which is generally pumped down by an SIP. } } Best Regards, } Yee Yan, Tay } FACTS Lab } Nanyang Technological University, Singapore } } ==============================Original Headers============================== } 3, 30 -- From rongchigram79-at-yahoo.com.sg Wed Oct 22 11:30:43 2014 } 3, 30 -- Received: from nm34-vm9.bullet.mail.sg3.yahoo.com (nm34-vm9.bullet.mail.sg3.yahoo.com [106.10.151.248]) } 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MGUf7Y008820 } 3, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 11:30:42 -0500 } 3, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1413995440; bh=5tlDQoN3Z58fFg6c7Rv75VnrB1xwxky6xMchEqpCwjs=; h=Date:From:Reply-To:To:Subject:From:Subject; b=aX1JCIHgVEECD6BtjZeuZJVj0K20sa45+OGvSD8EfPFqZhjY5flPGIUFjorrETPldjP2JFGPZnuBvbR4WTnCEZLsKWd2MOgiTXR6VQWcvkWlQwkHoaSCcKU6IsVizsSNfTjtPcp52oyyLYG1mEKibPf+hdQAFb7i2CRgoixP9anVIxiDB/bIe3+KMU0htpCoBczp6ouduF65FMfCHJ+z+fEVjDWbLQ0yzX3hMkNuyXB+AnexHJB+ggaBV/UcM+rq3J6s9drndZL9S2IpsdHo+s6XrB7B9cjWr/KIzcQ2+XUHJKTU9WmlodEIQugqV0OkZ9l9j2FF80yess6jDj2Rog== } 3, 30 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s2048; d=yahoo.com.sg; } 3, 30 -- b=e+jM7fUgcKY6y9g++uV2qdMSTgsYnCyLTDgeI4q6B9Uljx1gAYqsa+3TxeCGV7/DPNN+yWz1xK6oak+vBBZTB6u9La7nyNc+D0jPOq8H/QcneQhNHCwzHTiVR3vXmKWpP+2E6+0kW7z5fcfVya5MwfImxdCmBDIeKFKKYjKBjGi8BCDSjIphWL7wlO6Ruo3JkXR144b3kq8PHPioAxuaTsn94uiZX/OovlrN6ipGrlKt0suE4gvS4iP2fbeWQUg8U1JJDQ66Lhp82YqbKwHX4NK56J+hSwaWw9bZGfqjua9biUqWQW118tia1WTmUDgalpfuWk389KzzAQgO8Ya9Pg==; } 3, 30 -- Received: from [106.10.166.126] by nm34.bullet.mail.sg3.yahoo.com with NNFMP; 22 Oct 2014 16:30:40 -0000 } 3, 30 -- Received: from [106.10.151.254] by tm15.bullet.mail.sg3.yahoo.com with NNFMP; 22 Oct 2014 16:30:40 -0000 } 3, 30 -- Received: from [127.0.0.1] by omp1003.mail.sg3.yahoo.com with NNFMP; 22 Oct 2014 16:30:40 -0000 } 3, 30 -- X-Yahoo-Newman-Property: ymail-3 } 3, 30 -- X-Yahoo-Newman-Id: 604926.43057.bm-at-omp1003.mail.sg3.yahoo.com } 3, 30 -- X-YMail-OSG: uhz1Qd0VM1nVqESgXsqchFlV7FDr4UxSGR8mHLZy4SJi8r7CAdLWvsUwf378XjX } 3, 30 -- qYDSUJ04h94mgZfApTCoA3RkFXFLQ5W2WKrH1XoRhGzOFx9UG_zszyL1uGv4Py.wKVU_1k3ZRL_. } 3, 30 -- BZSNb6YX3v7FvRq6IcpLLHQXJPAgi9cZTwvvPi2aWVHzXUMwXwbx3QOdizacmrX2WtlMQOvnxy5M } 3, 30 -- 9qitKNvMV7qfz5fzsETwDewMREkjTvCaGU27LbuE3DKAaUfOM6Nhtxwbad7gi8UoxyIu1.Mlfzrq } 3, 30 -- ed9S3szyq_XIGkad0.nYAdLhXIr6OW4MojlIJoHR9hGhfI3VW6Dnzbqdaaup_FKtVuaZ.UWgCMHP } 3, 30 -- kHxs6RoVqcEAv1mrUDUU17Rx.I_28OkFdgL463xCFY6qplCYGHHtu2s7p05IPYORfRnQx.7SvluB } 3, 30 -- eZ2yEAc5T9vX6EmBboBNeW3TBKJPhwOcZ1rCZWMbvl51zpWBLMZLYdw-- } 3, 30 -- Received: by 106.10.196.93; Wed, 22 Oct 2014 16:30:40 +0000 } 3, 30 -- Date: Wed, 22 Oct 2014 16:30:39 +0000 (UTC) } 3, 30 -- From: YY YY {rongchigram79-at-yahoo.com.sg} } 3, 30 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} } 3, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 3, 30 -- Message-ID: {368559147.402507.1413995439738.JavaMail.yahoo-at-jws10947.mail.sg3.yahoo.com} } 3, 30 -- Subject: Choosing a TMP Unit over a Diffusion Pump for the Camera Chamber in } 3, 30 -- TEM } 3, 30 -- MIME-Version: 1.0 } 3, 30 -- Content-Type: text/plain; charset=UTF-8 } 3, 30 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 29 -- From wim.hagen-at-me.com Wed Oct 22 13:27:38 2014 6, 29 -- Received: from nk11p04mm-asmtp001.mac.com (nk11p04mm-asmtp001.mac.com [17.158.236.236]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MIRcwE031347 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 13:27:38 -0500 6, 29 -- Received: from [192.168.2.112] 6, 29 -- (dslb-088-064-183-072.088.064.pools.vodafone-ip.de [88.64.183.72]) 6, 29 -- by nk11p04mm-asmtp001.mac.com 6, 29 -- (Oracle Communications Messaging Server 7u4-27.10(7.0.4.27.9) 64bit (built Jun 6, 29 -- 6 2014)) with ESMTPSA id {0NDU009SYZ9OU580-at-nk11p04mm-asmtp001.mac.com} for 6, 29 -- Microscopy-at-microscopy.com; Wed, 22 Oct 2014 18:27:28 +0000 (GMT) 6, 29 -- X-Proofpoint-Virus-Version: vendor=fsecure 6, 29 -- engine=2.50.10432:5.12.52,1.0.28,0.0.0000 6, 29 -- definitions=2014-10-22_07:2014-10-22,2014-10-22,1970-01-01 signatures=0 6, 29 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 6, 29 -- suspectscore=15 phishscore=0 adultscore=0 bulkscore=0 classifier=spam adjust=0 6, 29 -- reason=mlx scancount=1 engine=7.0.1-1408290000 definitions=main-1410220168 6, 29 -- Subject: Re: [Microscopy] Choosing a TMP Unit over a Diffusion Pump for the 6, 29 -- Camera Chamber in 6, 29 -- References: {201410221642.s9MGgaBi023234-at-ns.microscopy.com} 6, 29 -- From: Wim Hagen {wim.hagen-at-me.com} 6, 29 -- Content-type: text/plain; charset=us-ascii 6, 29 -- X-Mailer: iPod touch Mail (11D257) 6, 29 -- In-reply-to: {201410221642.s9MGgaBi023234-at-ns.microscopy.com} 6, 29 -- Message-id: {91A5C6CB-FD5C-42AA-BABD-A5F179004B64-at-me.com} 6, 29 -- Date: Wed, 22 Oct 2014 20:27:22 +0200 6, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 29 -- MIME-version: 1.0 (1.0) 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9MIRcwE031347 ==============================End of - Headers==============================
I am actually getting ready to replace the diff pump on my antique Hitachi S-450 SEM with a turbo.
Turbos have many advantages, no back streaming from the pump, you still may have back streaming from the roughing pump, though. Most modern turbo pumps are actually turbo-drag pumps, they have a molecular drag stage after the turbo stage which allows much higher backing pressures than a traditional turbo pump or a diffusion pump, this means you can get away with a cheaper rouging pump like a diaphragm pump or a scroll dry pump. Using these pumps mean there will be zero oil back streaming into the chamber.
Also turbo pumps spool up much faster than a diff pump warms up. The little pump I am putting on my SEM spins up in about 1 minute, the diff pump takes about 20 minutes to warm up. There is also probably no need for cooling on the turbo pump unless you are cycling the chamber a lot. Oh, and the working fluid in the diff pump is incredibly expensive for a SEM, most use Santovac 5 which is something like $250 for 100ml.
Now for the bad…. They are kind of delicate critters, a friend at Portland State University has a Leybold maglev pump grenade on him the other day. Ripped it right off the mount. Don’t know why it happened, must have been a failure in the maglev section which caused it to crash. Turbo pumps are much less tolerant of accidents than a diff pump, you let air into it and bad things happen with a turbo, with a diff pump you just get a mess.
There is also vibration, even the maglev pumps have some vibration. This is usually remedied with weighted isolation bellows.
-Jerry
} On Oct 22, 2014, at 9:45 AM, rongchigram79-at-yahoo.com.sg wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, I would like to hear from you advices if one will prefably choose a TMP unit over a diffusion pump for the camera chamber. So far, i learn that TMP will take a shorter time to pump down the chamber. May i ask if there are many other advantages of using TMP over the diffusion pump? } } Will it also reduce carbon contamination on the sample even though the sample should be in sample column area which is generally pumped down by an SIP. } } Best Regards, } Yee Yan, Tay } FACTS Lab } Nanyang Technological University, Singapore } } ==============================Original Headers============================== } 3, 30 -- From rongchigram79-at-yahoo.com.sg Wed Oct 22 11:30:43 2014 } 3, 30 -- Received: from nm34-vm9.bullet.mail.sg3.yahoo.com (nm34-vm9.bullet.mail.sg3.yahoo.com [106.10.151.248]) } 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MGUf7Y008820 } 3, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 11:30:42 -0500 } 3, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1413995440; bh=5tlDQoN3Z58fFg6c7Rv75VnrB1xwxky6xMchEqpCwjs=; h=Date:From:Reply-To:To:Subject:From:Subject; b=aX1JCIHgVEECD6BtjZeuZJVj0K20sa45+OGvSD8EfPFqZhjY5flPGIUFjorrETPldjP2JFGPZnuBvbR4WTnCEZLsKWd2MOgiTXR6VQWcvkWlQwkHoaSCcKU6IsVizsSNfTjtPcp52oyyLYG1mEKibPf+hdQAFb7i2CRgoixP9anVIxiDB/bIe3+KMU0htpCoBczp6ouduF65FMfCHJ+z+fEVjDWbLQ0yzX3hMkNuyXB+AnexHJB+ggaBV/UcM+rq3J6s9drndZL9S2IpsdHo+s6XrB7B9cjWr/KIzcQ2+XUHJKTU9WmlodEIQugqV0OkZ9l9j2FF80yess6jDj2Rog== } 3, 30 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s2048; d=yahoo.com.sg; } 3, 30 -- b=e+jM7fUgcKY6y9g++uV2qdMSTgsYnCyLTDgeI4q6B9Uljx1gAYqsa+3TxeCGV7/DPNN+yWz1xK6oak+vBBZTB6u9La7nyNc+D0jPOq8H/QcneQhNHCwzHTiVR3vXmKWpP+2E6+0kW7z5fcfVya5MwfImxdCmBDIeKFKKYjKBjGi8BCDSjIphWL7wlO6Ruo3JkXR144b3kq8PHPioAxuaTsn94uiZX/OovlrN6ipGrlKt0suE4gvS4iP2fbeWQUg8U1JJDQ66Lhp82YqbKwHX4NK56J+hSwaWw9bZGfqjua9biUqWQW118tia1WTmUDgalpfuWk389KzzAQgO8Ya9Pg==; } 3, 30 -- Received: from [106.10.166.126] by nm34.bullet.mail.sg3.yahoo.com with NNFMP; 22 Oct 2014 16:30:40 -0000 } 3, 30 -- Received: from [106.10.151.254] by tm15.bullet.mail.sg3.yahoo.com with NNFMP; 22 Oct 2014 16:30:40 -0000 } 3, 30 -- Received: from [127.0.0.1] by omp1003.mail.sg3.yahoo.com with NNFMP; 22 Oct 2014 16:30:40 -0000 } 3, 30 -- X-Yahoo-Newman-Property: ymail-3 } 3, 30 -- X-Yahoo-Newman-Id: 604926.43057.bm-at-omp1003.mail.sg3.yahoo.com } 3, 30 -- X-YMail-OSG: uhz1Qd0VM1nVqESgXsqchFlV7FDr4UxSGR8mHLZy4SJi8r7CAdLWvsUwf378XjX } 3, 30 -- qYDSUJ04h94mgZfApTCoA3RkFXFLQ5W2WKrH1XoRhGzOFx9UG_zszyL1uGv4Py.wKVU_1k3ZRL_. } 3, 30 -- BZSNb6YX3v7FvRq6IcpLLHQXJPAgi9cZTwvvPi2aWVHzXUMwXwbx3QOdizacmrX2WtlMQOvnxy5M } 3, 30 -- 9qitKNvMV7qfz5fzsETwDewMREkjTvCaGU27LbuE3DKAaUfOM6Nhtxwbad7gi8UoxyIu1.Mlfzrq } 3, 30 -- ed9S3szyq_XIGkad0.nYAdLhXIr6OW4MojlIJoHR9hGhfI3VW6Dnzbqdaaup_FKtVuaZ.UWgCMHP } 3, 30 -- kHxs6RoVqcEAv1mrUDUU17Rx.I_28OkFdgL463xCFY6qplCYGHHtu2s7p05IPYORfRnQx.7SvluB } 3, 30 -- eZ2yEAc5T9vX6EmBboBNeW3TBKJPhwOcZ1rCZWMbvl51zpWBLMZLYdw-- } 3, 30 -- Received: by 106.10.196.93; Wed, 22 Oct 2014 16:30:40 +0000 } 3, 30 -- Date: Wed, 22 Oct 2014 16:30:39 +0000 (UTC) } 3, 30 -- From: YY YY {rongchigram79-at-yahoo.com.sg} } 3, 30 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} } 3, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 3, 30 -- Message-ID: {368559147.402507.1413995439738.JavaMail.yahoo-at-jws10947.mail.sg3.yahoo.com} } 3, 30 -- Subject: Choosing a TMP Unit over a Diffusion Pump for the Camera Chamber in } 3, 30 -- TEM } 3, 30 -- MIME-Version: 1.0 } 3, 30 -- Content-Type: text/plain; charset=UTF-8 } 3, 30 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 36 -- From jerry.biehler-at-gmail.com Wed Oct 22 14:20:07 2014 9, 36 -- Received: from mail-pa0-f45.google.com (mail-pa0-f45.google.com [209.85.220.45]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MJK68s019279 9, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 14:20:06 -0500 9, 36 -- Received: by mail-pa0-f45.google.com with SMTP id lj1so4284939pab.18 9, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 12:20:06 -0700 (PDT) 9, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 36 -- d=gmail.com; s=20120113; 9, 36 -- h=content-type:mime-version:subject:from:in-reply-to:date 9, 36 -- :content-transfer-encoding:message-id:references:to; 9, 36 -- bh=wMoxtkv5par6E9Oja67jXe2/VRdNdyjUKoAjtxcPqqE=; 9, 36 -- b=MA8pAXGd56C6vKguqXQJy4lUbw7DCaYQjpkSIHbPwdk7CW4GSAYQ27ia1bAl74zWQF 9, 36 -- ZdsjFcLKxVeMY8bGqZ19GsGe1Q8MS/RjnPOGalRuq5TPgJxpxqATCtnEPFeaNPLSm2bt 9, 36 -- FyboO4jYhL+jZTJmh72lb3lRf4390krHiOJtc0FwcOgMQ4Jik+k+70IiNAtyGejsiFcx 9, 36 -- ZFo/TJtK+fyRn7r3eTCXQzxRhzNcV/kva+cFPaCtDJtAz52dlBeXODe7XW/jcD7mtmU6 9, 36 -- znbu1WEAVjdBCVdrtQMrZKwbJApLwx8PB+KuhXoUyxmZBGoEhL6AVKByPms/PcZYoB04 9, 36 -- hgeA== 9, 36 -- X-Received: by 10.70.123.138 with SMTP id ma10mr166743pdb.64.1414005606197; 9, 36 -- Wed, 22 Oct 2014 12:20:06 -0700 (PDT) 9, 36 -- Received: from [10.0.38.131] (50-196-14-2-static.hfc.comcastbusiness.net. [50.196.14.2]) 9, 36 -- by mx.google.com with ESMTPSA id zn2sm14826969pbb.41.2014.10.22.12.20.05 9, 36 -- for {Microscopy-at-microscopy.com} 9, 36 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 9, 36 -- Wed, 22 Oct 2014 12:20:05 -0700 (PDT) 9, 36 -- Content-Type: text/plain; charset=utf-8 9, 36 -- Mime-Version: 1.0 (Mac OS X Mail 8.0 \(1990.1\)) 9, 36 -- Subject: Re: [Microscopy] Choosing a TMP Unit over a Diffusion Pump for the Camera Chamber in 9, 36 -- From: Jerry Biehler {jerry.biehler-at-gmail.com} 9, 36 -- In-Reply-To: {201410221645.s9MGjc3W028398-at-ns.microscopy.com} 9, 36 -- Date: Wed, 22 Oct 2014 12:20:04 -0700 9, 36 -- Message-Id: {06D32880-7364-46A1-9224-B46E9FDDF9A9-at-gmail.com} 9, 36 -- References: {201410221645.s9MGjc3W028398-at-ns.microscopy.com} 9, 36 -- To: Microscopy-at-microscopy.com 9, 36 -- X-Mailer: Apple Mail (2.1990.1) 9, 36 -- Content-Transfer-Encoding: 8bit 9, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9MJK68s019279 ==============================End of - Headers==============================
I don't know if you left out some details, but if it were me, I would not accept just a statement from the architects. I would ask who they had do the site survey, what are their qualifications, where and when were the readings taken, and most of all, give me a copy of the report. I should also point out that I would have watched the vendor do their site survey, and required a copy of their report, also. I have learned not to flat out accept this type of information, and to try to be a little bit scientific in reviewing the information, especially if some would be selling me something, or avoiding expense while providing something for me.
I wish you success! Darrell
Rosemary.White-at-csiro.au wrote on 10/21/2014 09:18:58 PM:
} From: Rosemary.White-at-csiro.au } To: Darrell Miles/Fishkill/IBM-at-IBMUS } Date: 10/21/2014 09:19 PM } Subject: [Microscopy] site inspections - overriding vendor recommendations } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } We're about to move into a temporary facility, and before doing so had the } SEM vendor check the site to see if the vibration and electromagnetic } interference was within spec. The site has excess vibration from 0.1-3 Hz, } although it's not much over spec, but it was well out of spec for EMI. The } vendor recommended installing an active field cancelling system. } } So, the architects got their own electromagnetic inspection and lo and } behold, they said the site was OK, so we're not getting a field cancelling } system. We're definitely having an inspection before re-installation of } the instrument, but I'm a bit stymied as to what we'll do if it's very } much out of spec. } } Has this happened to anyone and how did you handle it? } } thanks, } Rosemary } } p.s. this email was rejected at first when in html format, not sure why. } } Dr Rosemary White } CSIRO Black Mountain } GPO Box 1600 } Canberra, ACT 2601 } Australia } } T 61 2 6246 5475 } F 61 2 6246 5334 } E rosemary.white-at-csiro.au } } } } ==============================Original Headers============================== } 10, 45 -- From prvs=365b606e5=Rosemary.White-at-csiro.au Tue Oct 21 20:17:59 2014 } 10, 45 -- Received: from act-MTAout2.csiro.au (act-MTAout2.csiro.au } [150.229.7.38]) } 10, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id s9M1HvvC002188 } 10, 45 -- for {microscopy-at-microscopy.com} ; Tue, 21 Oct 2014 20:17:58 -0500 } 10, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 10, 45 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; } 10, 45 -- t=1413940678; x=1445476678; } 10, 45 -- h=from:to:subject:date:message-id:content-id: } 10, 45 -- content-transfer-encoding:mime-version; } 10, 45 -- bh=KjMLyyW83W1Bfb1SJRATUgKGmQsPspFZHT2npuqDUcU=; } 10, 45 -- b=mq+FwGd69D/FLxPbjAg92fd1iMG1M+lXaKUxCK+2S7B6aIsxhRTMvC5u } 10, 45 -- H3XfdFJjp40IiMdOTPw49R6Vy5B8sDP762wk8E5/kTyxX0BpFdK5GUXUO } 10, 45 -- 6vwR57fuivXFvU+; } 10, 45 -- X-SBRS: 4.5 } 10, 45 -- X-IronPort-Anti-Spam-Filtered: true } 10, 45 -- X-IronPort-Anti-Spam-Result: Ag0WAFwFR1SYU/ } cWkWdsb2JhbABcgmyBTwTSe4JxFgEDDgEBAQEUEhQuhAk6UQE } +QicEE4hAAaFYpGKPdREBbYQ1BZ1dmiFsgQYEBRcEHoEDAQEB } 10, 45 -- X-IPAS-Result: Ag0WAFwFR1SYU/ } cWkWdsb2JhbABcgmyBTwTSe4JxFgEDDgEBAQEUEhQuhAk6UQE } +QicEE4hAAaFYpGKPdREBbYQ1BZ1dmiFsgQYEBRcEHoEDAQEB } 10, 45 -- X-IronPort-AV: E=Sophos;i="5.04,766,1406556000"; } 10, 45 -- d="scan'208";a="12897562" } 10, 45 -- Received: from exhtca02-cdc.nexus.csiro.au ([152.83.247.22]) } 10, 45 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES128-SHA; } 22 Oct 2014 12:17:53 +1100 } 10, 45 -- Received: from EXHTCA05-CDC.nexus.csiro.au (2405:b000:601: } 13::247:25) by } 10, 45 -- ExHTCA02-CDC.nexus.csiro.au (2405:b000:601:13::247:22) } with Microsoft SMTP } 10, 45 -- Server (TLS) id 14.3.195.1; Wed, 22 Oct 2014 12:17:53 +1100 } 10, 45 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80:: } 9de3:d7af:4ed1:b7b6]) by } 10, 45 -- exhtca05-cdc.nexus.csiro.au ([fe80::fde4:64ec:c03d:871% } 13]) with mapi id } 10, 45 -- 14.03.0195.001; Wed, 22 Oct 2014 12:17:53 +1100 } 10, 45 -- From: {Rosemary.White-at-csiro.au} } 10, 45 -- To: {microscopy-at-microscopy.com} } 10, 45 -- Subject: site inspections - overriding vendor recommendations } 10, 45 -- Thread-Topic: site inspections - overriding vendor recommendations } 10, 45 -- Thread-Index: AQHP7ZYAWFzZ//eo4EybckG7DX/t9w== } 10, 45 -- Date: Wed, 22 Oct 2014 01:17:52 +0000 } 10, 45 -- Message-ID: {D06D50D2.174A4E%rosemary.white-at-csiro.au} } 10, 45 -- Accept-Language: en-NZ, en-US, en-AU } 10, 45 -- Content-Language: en-US } 10, 45 -- X-MS-Has-Attach: } 10, 45 -- X-MS-TNEF-Correlator: } 10, 45 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 } 10, 45 -- x-originating-ip: [152.83.195.117] } 10, 45 -- Content-Type: text/plain; charset="us-ascii" } 10, 45 -- Content-ID: {B58534FDC2B08340916D8D341D106B1C-at-csiro.au} } 10, 45 -- MIME-Version: 1.0 } 10, 45 -- Content-Transfer-Encoding: 8bit } 10, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id s9M1HvvC002188 } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 42 -- From milesd-at-us.ibm.com Wed Oct 22 14:36:39 2014 8, 42 -- Received: from e7.ny.us.ibm.com (e7.ny.us.ibm.com [32.97.182.137]) 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MJadGW006475 8, 42 -- for {microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 14:36:39 -0500 8, 42 -- Received: from /spool/local 8, 42 -- by e7.ny.us.ibm.com with IBM ESMTP SMTP Gateway: Authorized Use Only! Violators will be prosecuted 8, 42 -- for {microscopy-at-microscopy.com} from {milesd-at-us.ibm.com} ; 8, 42 -- Wed, 22 Oct 2014 15:36:36 -0400 8, 42 -- Received: from d01dlp03.pok.ibm.com (9.56.250.168) 8, 42 -- by e7.ny.us.ibm.com (192.168.1.107) with IBM ESMTP SMTP Gateway: Authorized Use Only! Violators will be prosecuted; 8, 42 -- Wed, 22 Oct 2014 15:36:36 -0400 8, 42 -- Received: from b01cxnp22035.gho.pok.ibm.com (b01cxnp22035.gho.pok.ibm.com [9.57.198.25]) 8, 42 -- by d01dlp03.pok.ibm.com (Postfix) with ESMTP id A1A99C9003E 8, 42 -- for {Microscopy-at-Microscopy.Com} ; Wed, 22 Oct 2014 15:25:18 -0400 (EDT) 8, 42 -- Received: from d01av02.pok.ibm.com (d01av02.pok.ibm.com [9.56.224.216]) 8, 42 -- by b01cxnp22035.gho.pok.ibm.com (8.14.9/8.14.9/NCO v10.0) with ESMTP id s9MJaYNf3015060 8, 42 -- for {Microscopy-at-Microscopy.Com} ; Wed, 22 Oct 2014 19:36:34 GMT 8, 42 -- Received: from d01av02.pok.ibm.com (localhost [127.0.0.1]) 8, 42 -- by d01av02.pok.ibm.com (8.14.4/8.14.4/NCO v10.0 AVout) with ESMTP id s9MJaY1M017654 8, 42 -- for {Microscopy-at-Microscopy.Com} ; Wed, 22 Oct 2014 15:36:34 -0400 8, 42 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.63.8.33]) 8, 42 -- by d01av02.pok.ibm.com (8.14.4/8.14.4/NCO v10.0 AVin) with ESMTP id s9MJaY92017639; 8, 42 -- Wed, 22 Oct 2014 15:36:34 -0400 8, 42 -- In-Reply-To: {201410220118.s9M1IwuT002958-at-ns.microscopy.com} 8, 42 -- References: {201410220118.s9M1IwuT002958-at-ns.microscopy.com} 8, 42 -- To: Rosemary.White-at-csiro.au 8, 42 -- Cc: Microscopy-at-microscopy.com 8, 42 -- MIME-Version: 1.0 8, 42 -- Subject: Re: [Microscopy] site inspections - overriding vendor recommendations 8, 42 -- X-KeepSent: 6D4F5B06:B7F37D23-85257D79:006A1A5A; 8, 42 -- type=4; name=$KeepSent 8, 42 -- X-Mailer: IBM Notes Release 9.0.1SHF211 December 19, 2013 8, 42 -- Message-ID: {OF6D4F5B06.B7F37D23-ON85257D79.006A1A5A-85257D79.006BAF12-at-us.ibm.com} 8, 42 -- From: Darrell Miles {milesd-at-us.ibm.com} 8, 42 -- Date: Wed, 22 Oct 2014 15:36:11 -0400 8, 42 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 9.0.1FP1|April 03, 2014) at 8, 42 -- 10/22/2014 15:36:36, 8, 42 -- Serialize complete at 10/22/2014 15:36:36 8, 42 -- Content-Type: text/plain; charset="US-ASCII" 8, 42 -- X-TM-AS-MML: disable 8, 42 -- X-Content-Scanned: Fidelis XPS MAILER 8, 42 -- x-cbid: 14102219-0025-0000-0000-000000D35648 ==============================End of - Headers==============================
The vendor will not have to show any resolution if their measurement shows high fields, they will ask you to sign a waiver and any performance specs are out the door.
Try to get measurements over time, mornings, afternoons, evenings and also weekends:
I worked in a big city struggling with fields due to work on power lines two blocks down the road (TEM system in basement on corner of building), Friday afternoon it was gone forever.
A big EM vendor was about to spent a lot of money for a field cancelation/shielding solution in their factory when the problem was found to be wrongly connected room lighting on the other side of the building. This was found by the timing, 7am till 6pm during weekdays gave fields and this timing was traced to some room which was automatically powered down for safety reasons.
So it could be that there are fields only once and a while, differing in strength, try to figure that out and then possibly trace down where it comes from by talking to building maintenance as they have a good feel for what happens when in the building. For the measurements it's best you get an independent expert, it will be worth the money. Alternatively ask your EM vendor to measure a few times during a full week. I think they should do that anyway, put in some black box that measures everything for a week and then have it analyzed by a professional.
Best,
Wim Hagen EMBL Heidelberg
} On Oct 22, 2014, at 3:32, Rosemary.White-at-csiro.au wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } We're about to move into a temporary facility, and before doing so had the } SEM vendor check the site to see if the vibration and electromagnetic } interference was within spec. The site has excess vibration from 0.1-3 Hz, } although it's not much over spec, but it was well out of spec for EMI. The } vendor recommended installing an active field cancelling system. } } So, the architects got their own electromagnetic inspection and lo and } behold, they said the site was OK, so we're not getting a field cancelling } system. We're definitely having an inspection before re-installation of } the instrument, but I'm a bit stymied as to what we'll do if it's very } much out of spec. } } Has this happened to anyone and how did you handle it? } } thanks, } Rosemary } } p.s. this email was rejected at first when in html format, not sure why. } } Dr Rosemary White } CSIRO Black Mountain } GPO Box 1600 } Canberra, ACT 2601 } Australia } } T 61 2 6246 5475 } F 61 2 6246 5334 } E rosemary.white-at-csiro.au } } } } ==============================Original Headers============================== } 10, 45 -- From prvs=365b606e5=Rosemary.White-at-csiro.au Tue Oct 21 20:17:59 2014 } 10, 45 -- Received: from act-MTAout2.csiro.au (act-MTAout2.csiro.au [150.229.7.38]) } 10, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9M1HvvC002188 } 10, 45 -- for {microscopy-at-microscopy.com} ; Tue, 21 Oct 2014 20:17:58 -0500 } 10, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 10, 45 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; } 10, 45 -- t=1413940678; x=1445476678; } 10, 45 -- h=from:to:subject:date:message-id:content-id: } 10, 45 -- content-transfer-encoding:mime-version; } 10, 45 -- bh=KjMLyyW83W1Bfb1SJRATUgKGmQsPspFZHT2npuqDUcU=; } 10, 45 -- b=mq+FwGd69D/FLxPbjAg92fd1iMG1M+lXaKUxCK+2S7B6aIsxhRTMvC5u } 10, 45 -- H3XfdFJjp40IiMdOTPw49R6Vy5B8sDP762wk8E5/kTyxX0BpFdK5GUXUO } 10, 45 -- 6vwR57fuivXFvU+; } 10, 45 -- X-SBRS: 4.5 } 10, 45 -- X-IronPort-Anti-Spam-Filtered: true } 10, 45 -- X-IronPort-Anti-Spam-Result: Ag0WAFwFR1SYU/cWkWdsb2JhbABcgmyBTwTSe4JxFgEDDgEBAQEUEhQuhAk6UQE+QicEE4hAAaFYpGKPdREBbYQ1BZ1dmiFsgQYEBRcEHoEDAQEB } 10, 45 -- X-IPAS-Result: Ag0WAFwFR1SYU/cWkWdsb2JhbABcgmyBTwTSe4JxFgEDDgEBAQEUEhQuhAk6UQE+QicEE4hAAaFYpGKPdREBbYQ1BZ1dmiFsgQYEBRcEHoEDAQEB } 10, 45 -- X-IronPort-AV: E=Sophos;i="5.04,766,1406556000"; } 10, 45 -- d="scan'208";a="12897562" } 10, 45 -- Received: from exhtca02-cdc.nexus.csiro.au ([152.83.247.22]) } 10, 45 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES128-SHA; 22 Oct 2014 12:17:53 +1100 } 10, 45 -- Received: from EXHTCA05-CDC.nexus.csiro.au (2405:b000:601:13::247:25) by } 10, 45 -- ExHTCA02-CDC.nexus.csiro.au (2405:b000:601:13::247:22) with Microsoft SMTP } 10, 45 -- Server (TLS) id 14.3.195.1; Wed, 22 Oct 2014 12:17:53 +1100 } 10, 45 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80::9de3:d7af:4ed1:b7b6]) by } 10, 45 -- exhtca05-cdc.nexus.csiro.au ([fe80::fde4:64ec:c03d:871%13]) with mapi id } 10, 45 -- 14.03.0195.001; Wed, 22 Oct 2014 12:17:53 +1100 } 10, 45 -- From: {Rosemary.White-at-csiro.au} } 10, 45 -- To: {microscopy-at-microscopy.com} } 10, 45 -- Subject: site inspections - overriding vendor recommendations } 10, 45 -- Thread-Topic: site inspections - overriding vendor recommendations } 10, 45 -- Thread-Index: AQHP7ZYAWFzZ//eo4EybckG7DX/t9w== } 10, 45 -- Date: Wed, 22 Oct 2014 01:17:52 +0000 } 10, 45 -- Message-ID: {D06D50D2.174A4E%rosemary.white-at-csiro.au} } 10, 45 -- Accept-Language: en-NZ, en-US, en-AU } 10, 45 -- Content-Language: en-US } 10, 45 -- X-MS-Has-Attach: } 10, 45 -- X-MS-TNEF-Correlator: } 10, 45 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 } 10, 45 -- x-originating-ip: [152.83.195.117] } 10, 45 -- Content-Type: text/plain; charset="us-ascii" } 10, 45 -- Content-ID: {B58534FDC2B08340916D8D341D106B1C-at-csiro.au} } 10, 45 -- MIME-Version: 1.0 } 10, 45 -- Content-Transfer-Encoding: 8bit } 10, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9M1HvvC002188 } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 28 -- From wim.hagen-at-me.com Wed Oct 22 15:09:34 2014 11, 28 -- Received: from nk11p04mm-asmtp001.mac.com (nk11p04mm-asmtp001.mac.com [17.158.236.236]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MK9XiV026752 11, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 15:09:33 -0500 11, 28 -- Received: from [192.168.2.112] 11, 28 -- (dslb-088-064-183-072.088.064.pools.vodafone-ip.de [88.64.183.72]) 11, 28 -- by nk11p04mm-asmtp001.mac.com 11, 28 -- (Oracle Communications Messaging Server 7u4-27.10(7.0.4.27.9) 64bit (built Jun 11, 28 -- 6 2014)) with ESMTPSA id {0NDV000EY3ZC6I10-at-nk11p04mm-asmtp001.mac.com} for 11, 28 -- Microscopy-at-microscopy.com; Wed, 22 Oct 2014 20:09:17 +0000 (GMT) 11, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure 11, 28 -- engine=2.50.10432:5.12.52,1.0.28,0.0.0000 11, 28 -- definitions=2014-10-22_07:2014-10-22,2014-10-22,1970-01-01 signatures=0 11, 28 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 11, 28 -- suspectscore=25 phishscore=0 adultscore=0 bulkscore=0 classifier=spam adjust=0 11, 28 -- reason=mlx scancount=1 engine=7.0.1-1408290000 definitions=main-1410220185 11, 28 -- Subject: Re: [Microscopy] site inspections - overriding vendor recommendations 11, 28 -- References: {201410220132.s9M1WhTS017382-at-ns.microscopy.com} 11, 28 -- From: Wim Hagen {wim.hagen-at-me.com} 11, 28 -- Content-type: text/plain; charset=us-ascii 11, 28 -- X-Mailer: iPod touch Mail (11D257) 11, 28 -- In-reply-to: {201410220132.s9M1WhTS017382-at-ns.microscopy.com} 11, 28 -- Message-id: {6967A1A4-D149-4F9E-B762-8763202EDAFF-at-me.com} 11, 28 -- Date: Wed, 22 Oct 2014 22:09:09 +0200 11, 28 -- To: Microscopy-at-microscopy.com 11, 28 -- MIME-version: 1.0 (1.0) 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9MK9XiV026752 ==============================End of - Headers==============================
I wouldn't put too much credence in the vendor's survey. The maintenance engineers have no special training or expertise in analyzing or mitigating these environmental problems. In our case, for TEM, it turns out the EMI from the power supply is well above the threshold that was imposed. I see no evidence that this affects our performance.
I don't recall having to sign a waiver, but I may have forgotten. ------------------------------------------ Phil Ahrenkiel
-----Original Message----- X-from: wim.hagen-at-me.com [mailto:wim.hagen-at-me.com] Sent: Wednesday, October 22, 2014 2:14 PM To: Ahrenkiel, Phil
Dear Rosemary.
I confirm the many replies so far:
The vendor will not have to show any resolution if their measurement shows high fields, they will ask you to sign a waiver and any performance specs are out the door.
Try to get measurements over time, mornings, afternoons, evenings and also weekends:
I worked in a big city struggling with fields due to work on power lines two blocks down the road (TEM system in basement on corner of building), Friday afternoon it was gone forever.
A big EM vendor was about to spent a lot of money for a field cancelation/shielding solution in their factory when the problem was found to be wrongly connected room lighting on the other side of the building. This was found by the timing, 7am till 6pm during weekdays gave fields and this timing was traced to some room which was automatically powered down for safety reasons.
So it could be that there are fields only once and a while, differing in strength, try to figure that out and then possibly trace down where it comes from by talking to building maintenance as they have a good feel for what happens when in the building. For the measurements it's best you get an independent expert, it will be worth the money. Alternatively ask your EM vendor to measure a few times during a full week. I think they should do that anyway, put in some black box that measures everything for a week and then have it analyzed by a professional.
Best,
Wim Hagen EMBL Heidelberg
} On Oct 22, 2014, at 3:32, Rosemary.White-at-csiro.au wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } We're about to move into a temporary facility, and before doing so had the } SEM vendor check the site to see if the vibration and electromagnetic } interference was within spec. The site has excess vibration from 0.1-3 Hz, } although it's not much over spec, but it was well out of spec for EMI. The } vendor recommended installing an active field cancelling system. } } So, the architects got their own electromagnetic inspection and lo and } behold, they said the site was OK, so we're not getting a field cancelling } system. We're definitely having an inspection before re-installation of } the instrument, but I'm a bit stymied as to what we'll do if it's very } much out of spec. } } Has this happened to anyone and how did you handle it? } } thanks, } Rosemary } } p.s. this email was rejected at first when in html format, not sure why. } } Dr Rosemary White } CSIRO Black Mountain } GPO Box 1600 } Canberra, ACT 2601 } Australia } } T 61 2 6246 5475 } F 61 2 6246 5334 } E rosemary.white-at-csiro.au } } } } ==============================Original Headers============================== } 10, 45 -- From prvs=365b606e5=Rosemary.White-at-csiro.au Tue Oct 21 20:17:59 2014 } 10, 45 -- Received: from act-MTAout2.csiro.au (act-MTAout2.csiro.au [150.229.7.38]) } 10, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9M1HvvC002188 } 10, 45 -- for {microscopy-at-microscopy.com} ; Tue, 21 Oct 2014 20:17:58 -0500 } 10, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 10, 45 -- d=csiro.au; i=-at-csiro.au; q=dns/txt; s=email; } 10, 45 -- t=1413940678; x=1445476678; } 10, 45 -- h=from:to:subject:date:message-id:content-id: } 10, 45 -- content-transfer-encoding:mime-version; } 10, 45 -- bh=KjMLyyW83W1Bfb1SJRATUgKGmQsPspFZHT2npuqDUcU=; } 10, 45 -- b=mq+FwGd69D/FLxPbjAg92fd1iMG1M+lXaKUxCK+2S7B6aIsxhRTMvC5u } 10, 45 -- H3XfdFJjp40IiMdOTPw49R6Vy5B8sDP762wk8E5/kTyxX0BpFdK5GUXUO } 10, 45 -- 6vwR57fuivXFvU+; } 10, 45 -- X-SBRS: 4.5 } 10, 45 -- X-IronPort-Anti-Spam-Filtered: true } 10, 45 -- X-IronPort-Anti-Spam-Result: Ag0WAFwFR1SYU/cWkWdsb2JhbABcgmyBTwTSe4JxFgEDDgEBAQEUEhQuhAk6UQE+QicEE4hAAaFYpGKPdREBbYQ1BZ1dmiFsgQYEBRcEHoEDAQEB } 10, 45 -- X-IPAS-Result: Ag0WAFwFR1SYU/cWkWdsb2JhbABcgmyBTwTSe4JxFgEDDgEBAQEUEhQuhAk6UQE+QicEE4hAAaFYpGKPdREBbYQ1BZ1dmiFsgQYEBRcEHoEDAQEB } 10, 45 -- X-IronPort-AV: E=Sophos;i="5.04,766,1406556000"; } 10, 45 -- d="scan'208";a="12897562" } 10, 45 -- Received: from exhtca02-cdc.nexus.csiro.au ([152.83.247.22]) } 10, 45 -- by act-ironport-int.csiro.au with ESMTP/TLS/AES128-SHA; 22 Oct 2014 12:17:53 +1100 } 10, 45 -- Received: from EXHTCA05-CDC.nexus.csiro.au (2405:b000:601:13::247:25) by } 10, 45 -- ExHTCA02-CDC.nexus.csiro.au (2405:b000:601:13::247:22) with Microsoft SMTP } 10, 45 -- Server (TLS) id 14.3.195.1; Wed, 22 Oct 2014 12:17:53 +1100 } 10, 45 -- Received: from EXMBX04-CDC.nexus.csiro.au ([fe80::9de3:d7af:4ed1:b7b6]) by } 10, 45 -- exhtca05-cdc.nexus.csiro.au ([fe80::fde4:64ec:c03d:871%13]) with mapi id } 10, 45 -- 14.03.0195.001; Wed, 22 Oct 2014 12:17:53 +1100 } 10, 45 -- From: {Rosemary.White-at-csiro.au} } 10, 45 -- To: {microscopy-at-microscopy.com} } 10, 45 -- Subject: site inspections - overriding vendor recommendations } 10, 45 -- Thread-Topic: site inspections - overriding vendor recommendations } 10, 45 -- Thread-Index: AQHP7ZYAWFzZ//eo4EybckG7DX/t9w== } 10, 45 -- Date: Wed, 22 Oct 2014 01:17:52 +0000 } 10, 45 -- Message-ID: {D06D50D2.174A4E%rosemary.white-at-csiro.au} } 10, 45 -- Accept-Language: en-NZ, en-US, en-AU } 10, 45 -- Content-Language: en-US } 10, 45 -- X-MS-Has-Attach: } 10, 45 -- X-MS-TNEF-Correlator: } 10, 45 -- user-agent: Microsoft-MacOutlook/14.10.0.110310 } 10, 45 -- x-originating-ip: [152.83.195.117] } 10, 45 -- Content-Type: text/plain; charset="us-ascii" } 10, 45 -- Content-ID: {B58534FDC2B08340916D8D341D106B1C-at-csiro.au} } 10, 45 -- MIME-Version: 1.0 } 10, 45 -- Content-Transfer-Encoding: 8bit } 10, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9M1HvvC002188 } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 28 -- From wim.hagen-at-me.com Wed Oct 22 15:09:34 2014 11, 28 -- Received: from nk11p04mm-asmtp001.mac.com (nk11p04mm-asmtp001.mac.com [17.158.236.236]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MK9XiV026752 11, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Oct 2014 15:09:33 -0500 11, 28 -- Received: from [192.168.2.112] 11, 28 -- (dslb-088-064-183-072.088.064.pools.vodafone-ip.de [88.64.183.72]) 11, 28 -- by nk11p04mm-asmtp001.mac.com 11, 28 -- (Oracle Communications Messaging Server 7u4-27.10(7.0.4.27.9) 64bit (built Jun 11, 28 -- 6 2014)) with ESMTPSA id {0NDV000EY3ZC6I10-at-nk11p04mm-asmtp001.mac.com} for 11, 28 -- Microscopy-at-microscopy.com; Wed, 22 Oct 2014 20:09:17 +0000 (GMT) 11, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure 11, 28 -- engine=2.50.10432:5.12.52,1.0.28,0.0.0000 11, 28 -- definitions=2014-10-22_07:2014-10-22,2014-10-22,1970-01-01 signatures=0 11, 28 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 11, 28 -- suspectscore=25 phishscore=0 adultscore=0 bulkscore=0 classifier=spam adjust=0 11, 28 -- reason=mlx scancount=1 engine=7.0.1-1408290000 definitions=main-1410220185 11, 28 -- Subject: Re: [Microscopy] site inspections - overriding vendor recommendations 11, 28 -- References: {201410220132.s9M1WhTS017382-at-ns.microscopy.com} 11, 28 -- From: Wim Hagen {wim.hagen-at-me.com} 11, 28 -- Content-type: text/plain; charset=us-ascii 11, 28 -- X-Mailer: iPod touch Mail (11D257) 11, 28 -- In-reply-to: {201410220132.s9M1WhTS017382-at-ns.microscopy.com} 11, 28 -- Message-id: {6967A1A4-D149-4F9E-B762-8763202EDAFF-at-me.com} 11, 28 -- Date: Wed, 22 Oct 2014 22:09:09 +0200 11, 28 -- To: Microscopy-at-microscopy.com 11, 28 -- MIME-version: 1.0 (1.0) 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9MK9XiV026752 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 42 -- From Phil.Ahrenkiel-at-sdsmt.edu Wed Oct 22 17:43:02 2014 19, 42 -- Received: from silver.sdsmt.edu (silver.sdsmt.edu [151.159.1.1]) 19, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9MMh2Ek020208 19, 42 -- for {Microscopy-at-Microscopy.Com} ; Wed, 22 Oct 2014 17:43:02 -0500 19, 42 -- Received: from SDSMT-ex13.sdsmt.edu (sdsmt-ex13.sdsmt.edu [151.159.3.13]) 19, 42 -- by silver.sdsmt.edu (8.14.9/8.14.7) with ESMTP id s9MMh1Eg012553 19, 42 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=OK) 19, 42 -- for {Microscopy-at-Microscopy.Com} ; Wed, 22 Oct 2014 16:43:01 -0600 19, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sdsmt.edu; 19, 42 -- s=sdsmtdkim; t=1414017781; 19, 42 -- bh=aHrYtoXlKEoObeYNdwaZffgqNUKC7RcDfSvwRjJTjwo=; 19, 42 -- h=From:To:Subject:Date:References:In-Reply-To; 19, 42 -- b=Z0rGRDvafEf0HQC+hRMohdOt0TqxEglIjhsr4+d2BRKFyYnZti7lhmpc5PhwoQUne 19, 42 -- YciImjy/sRD3uvbB/3BJrZyWibbgkI704E/Il+pBcmVlNAYuPkXgT7cMAWGY4qkbLM 19, 42 -- QNQny3ajMB7aG4BAbelZclytyXKZJ4D/ss+YkJTQ= 19, 42 -- Received: from SDSMT-ex13.sdsmt.edu (2607:f558:1000:f::6d) by 19, 42 -- SDSMT-ex13.sdsmt.edu (2607:f558:1000:f::6d) with Microsoft SMTP Server (TLS) 19, 42 -- id 15.0.913.22; Wed, 22 Oct 2014 16:43:01 -0600 19, 42 -- Received: from SDSMT-ex13.sdsmt.edu ([fe80::1492:416b:26a0:40e8]) by 19, 42 -- SDSMT-ex13.sdsmt.edu ([fe80::1492:416b:26a0:40e8%15]) with mapi id 19, 42 -- 15.00.0913.011; Wed, 22 Oct 2014 16:43:01 -0600 19, 42 -- From: "Ahrenkiel, Phil" {Phil.Ahrenkiel-at-sdsmt.edu} 19, 42 -- To: "'Microscopy-at-Microscopy.Com' (Microscopy-at-Microscopy.Com)" 19, 42 -- {Microscopy-at-Microscopy.Com} 19, 42 -- Subject: RE: [Microscopy] Re: site inspections - overriding vendor 19, 42 -- recommendations 19, 42 -- Thread-Topic: [Microscopy] Re: site inspections - overriding vendor 19, 42 -- recommendations 19, 42 -- Thread-Index: AQHP7jTFpC2fnfVy1UGjJ4mkCvWaJpw8rSKw 19, 42 -- Date: Wed, 22 Oct 2014 22:43:00 +0000 19, 42 -- Message-ID: {3e71b8ac35584fa08719bf1834b6f7db-at-SDSMT-ex13.sdsmt.edu} 19, 42 -- References: {201410222014.s9MKE8Zv000300-at-ns.microscopy.com} 19, 42 -- In-Reply-To: {201410222014.s9MKE8Zv000300-at-ns.microscopy.com} 19, 42 -- Accept-Language: en-US 19, 42 -- Content-Language: en-US 19, 42 -- X-MS-Has-Attach: 19, 42 -- X-MS-TNEF-Correlator: 19, 42 -- x-originating-ip: [2607:f558:1000:14:8354:c249:d1a5:933f] 19, 42 -- Content-Type: text/plain; charset="us-ascii" 19, 42 -- MIME-Version: 1.0 19, 42 -- Content-Transfer-Encoding: 8bit 19, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9MMh2Ek020208 ==============================End of - Headers==============================
There are many advantages to using a turbo pump, but the main reason I do not like them is the maintenance question. If you have a lab hundreds of miles from your nearest maintenance technician, you are better off with a diffusion pump. The great thing about a DP is that anyone with a mild degree of skill can fix it when it stops working! I hate areas of a machine that you cannot fix with over the phone help, when a turbo stops it stops, and you have to wait for an exchange unit.
A tricky decision, good luck!
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
-----Original Message----- X-from: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] Sent: 22 October 2014 17:32 To: protrain-at-emcourses.com
X-from: igor.levin-at-nist.gov ()
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Email: igor.levin-at-nist.gov Name: Igor Levin
Organization: NIST
Title-Subject: [Filtered] postdoctoral researcher in customization of TEM
Message: We are seeking a collaborative researcher, possibly at a senior postdoctoral level, to develop high precision, atomic-scale structural measurement techniques for the characterization of complex structures. Experience with electron microscopy and knowledge of the FEI TIA and Gatan Digital Micrograph scripting interfaces is required, as well as some experience with customization of electron-beam equipment. Some experience with computational image processing through Python, Matlab, C, etc. would also be of benefit. For further information, contact Igor Levin; igor.levin-at-nist.gov
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Email: michael_standing-at-byu.edu Name: Michael Standing
Organization: Brigham Young University
Title-Subject: [Filtered] Textbook on biological EM specimen preparation
Message: Hello everyone,
We are in the process of developing a biological EM sample preparation class. We haven't had this class available for a few years and the previous instructor didn't use a formal text. We now are looking for a textbook that will cover most of the basic preparation strategies for both SEM and TEM biological sample preparations. Any and all recommendations for texts that are currently in print would be very helpful for us.
Thanks in advance,
Mike ============================== Michael D. Standing BYU Microscopy Lab 108 MB Provo, UT 84602
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Oct 23 19:03:18 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9O03I8q027170 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 23 Oct 2014 19:03:18 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 65C38775585 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 23 Oct 2014 19:03:18 -0500 (CDT) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id wMRzb3im1pWB for {microscopy-at-microscopy.com} ; 13, 28 -- Thu, 23 Oct 2014 19:03:13 -0500 (CDT) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id 7A395775578 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 23 Oct 2014 19:03:13 -0500 (CDT) 13, 28 -- Message-ID: {54499741.4020900-at-microscopy.com} 13, 28 -- Date: Thu, 23 Oct 2014 19:03:13 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Textbook on biological EM specimen preparation 13, 28 -- References: {201410221404.s9ME4Qdu017400-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201410221404.s9ME4Qdu017400-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201410221404.s9ME4Qdu017400-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: kjo29-at-rockets.utoledo.edu Name: Jo
Organization: University of Toledo
Title-Subject: [Filtered] High Pressure Freezing conditions for TEM
Message: Hello I am trying to find the best condition for High pressure freezing and Freeze substitution methods that will keep the samples in the most native as possible. More specifically, im wondering about the filler/cryoprotectant usage for HPF, and ethanol or methanol for dehydration during Freeze substitution.
thank you
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Email: whiteto-at-missouri.edu Name: Tommi White
Organization: University of Missouri Electron Microscopy Core Facility
Title-Subject: [Filtered] Fall Meeting - Central States Microscopy & Microanalysis Society
Message: Central States Microscopy & Microanalysis Society will be having their Fall Meeting entitled "Looking Back to Move Forward" on November 5, 2014 at Missouri Science and Technology in Rolla, Missouri. Please see the following website for more details: http://www.emc.missouri.edu/csmms/ Looking forward to seeing you there, please let me know if you have any questions.
Tommi A. White, Ph.D. Assistant Professor of Biochemistry Associate Director, Electron Microscopy Core Facility University of Missouri W125 Veterinary Medicine Building 1600 East Rollins Street Columbia, MO 65211 573-882-8304 WhiteTo-at-missouri.edu www.emc.missouri.edu
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There is a nice and cheap book describing all TEM sample preparation in a synthetic way and with the support of an interactive web site:
/Sample Preparation Handbook for Transmission Electron Microscopy/ from Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub (vol1 /Methodology/, vol 2 /Techniques/).
The guide on the Web site can be run free, without the book, to choose a method. The book brings more details on the procedures.
Have a look to book: http://temsamprep.in2p3.fr/livre.php?lang=eng web site: http://temsamprep.in2p3.fr/livre.php?lang=eng basic guide: http://temsamprep.in2p3.fr/guides_methodologiques.php?lang=eng
Enjoy
Philippe Buffat
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I'm hoping for good replies myself. We haven't found any recent EM texts that cover biological prep for class use. We're still using John Bozzola's book for our TEM course, hoping that some day he'll get tired of retirement and write a new edition (you still read this list, John?). The only other texts I know of are Glauert & Lewis and Dystra and Reuss, but they're no newer.
There is a crying need for modern microscopy class texts, especially for life sciences. The new EM books are excellent references, but not great for class texts.
Phil
} Email: michael_standing-at-byu.edu } Name: Michael Standing } } Organization: Brigham Young University } } Title-Subject: [Filtered] Textbook on biological EM specimen preparation } } Message: Hello everyone, } } We are in the process of developing a biological EM sample preparation } class. We haven't had this class available for a few years and the } previous instructor didn't use a formal text. We now are looking for a } textbook that will cover most of the basic preparation strategies for both } SEM and TEM biological sample preparations. Any and all recommendations } for texts that are currently in print would be very helpful for us. } } Thanks in advance, } } Mike } ============================== } Michael D. Standing } BYU Microscopy Lab } 108 MB } Provo, UT 84602 } } Phone: 801-422-4011 } e-mail: michael_standing-at-byu.edu } ============================== -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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Message: We are looking for someone to take one or all of our three transmission electron microscopes. We have two cm10 units and one cm12. One cm10 is not 100% complete and was used for parts. The cm12 was up and running a few weeks ago before shutting it down. We are in the midst of moving our lab off campus and do not have need for the scopes anymore.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Oct 24 07:10:25 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9OCAPIh010408 12, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2014 07:10:25 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id 3075F775B5D 12, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2014 07:10:15 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id xseup0So9d4b for {microscopy-at-microscopy.com} ; 12, 28 -- Fri, 24 Oct 2014 07:09:39 -0500 (CDT) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id AC54E775B4A 12, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2014 07:09:39 -0500 (CDT) 12, 28 -- Message-ID: {544A4183.4000006-at-microscopy.com} 12, 28 -- Date: Fri, 24 Oct 2014 07:09:39 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:CM10 and CM12 available 12, 28 -- References: {201410240441.s9O4fPBk010841-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201410240441.s9O4fPBk010841-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201410240441.s9O4fPBk010841-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
As we all are aware, negative stains work by the fact that they consist of heavier elements that scatter electrons better than the sample. They surround the sample particles and pool in low areas or openings but penetrate the sample themselves minimally in a well prepared sample. However, some samples do much better than others in providing higher contrast between the sample and background than others.
It is possible to get positive staining with a negative stain but this is undesirable as it results in dark particles that tend to show little detail or contrast against the background,.
In my experience, those particles that are of tighter construction such as highly crystalline particles tend to take up less stain and thus appear sharper and better defined than those that are more porous or have less well defined edges in well-prepared negative stain preparations.
Do any of you know of any references that discuss negative staining and the anticipated effect on crystalline verses non-crystalline particles?
Debby
Debra Sherman, Founder & CSO DS imaging LLC Purdue Technology Center 1281 Win Hentschel Blvd West Lafayette, IN 47906 E-mail: debby.sherman-at-dsimagingllc.com www.dsimagingllc.com Mobile: 765-418-8540
==============================Original Headers============================== 9, 31 -- From dsherman-at-purdue.edu Mon Oct 27 07:35:56 2014 9, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9RCZuL9013147 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2014 07:35:56 -0500 9, 31 -- Received: from WPVEXCHUB01.purdue.lcl (wpvexchub01.itap.purdue.edu [172.30.136.61]) 9, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id s9RCZu6K024233 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2014 08:35:56 -0400 9, 31 -- Received: from WPVEXCMBX02.purdue.lcl ([172.30.136.65]) by 9, 31 -- WPVEXCHUB01.purdue.lcl ([172.30.136.61]) with mapi id 14.03.0174.001; Mon, 27 9, 31 -- Oct 2014 08:35:55 -0400 9, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 9, 31 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 9, 31 -- Subject: action of negative stain 9, 31 -- Thread-Topic: action of negative stain 9, 31 -- Thread-Index: AQHP8eKNyBTfJWwjr0uFoAo7eM7opw== 9, 31 -- Date: Mon, 27 Oct 2014 12:35:55 +0000 9, 31 -- Message-ID: {D073B460.2446A%debby.sherman-at-dsimagingllc.com} 9, 31 -- Accept-Language: en-US 9, 31 -- Content-Language: en-US 9, 31 -- X-MS-Has-Attach: 9, 31 -- X-MS-TNEF-Correlator: 9, 31 -- user-agent: Microsoft-MacOutlook/14.4.4.140807 9, 31 -- x-originating-ip: [204.52.32.196] 9, 31 -- Content-Type: text/plain; charset="us-ascii" 9, 31 -- Content-ID: {92442A48E621794FB93734A0BFBDA6EC-at-exchange.purdue.edu} 9, 31 -- MIME-Version: 1.0 9, 31 -- X-PMX-Version: 6.0.2.2308539 9, 31 -- X-PerlMx-URL-Scanned: Yes 9, 31 -- X-PerlMx-Virus-Scanned: Yes 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9RCZuL9013147 ==============================End of - Headers==============================
Hi, I am looking for a LEO 14xx SEM vacuum PCB and stage PCB boards for our SEM. Could you please let me know if you happen to have one of these boards for sale or if you know someone may have? Zeiss has the parts but won't sale unless to be installed by them. Your help is greatly appreciated.
Thank you. Ping
==============================Original Headers============================== 3, 19 -- From pli-at-dal.ca Mon Oct 27 10:58:20 2014 3, 19 -- Received: from kil-sm-1.ucis.dal.ca (KIL-SM-1.UCIS.Dal.Ca [129.173.1.130]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9RFwJoB002794 3, 19 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2014 10:58:19 -0500 3, 19 -- Received: from Ping1055 (PLi-9.Biology.Dal.Ca [129.173.33.7]) 3, 19 -- by kil-sm-1.ucis.dal.ca (8.14.2+Sun/8.14.2) with ESMTP id s9RFvcXm003508 3, 19 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2014 12:57:39 -0300 (ADT) 3, 19 -- From: "Ping Li" {pli-at-dal.ca} 3, 19 -- To: {microscopy-at-microscopy.com} 3, 19 -- Subject: Re: Looking for a LEO 14xx SEM vacuum PCB and stage PCB 3, 19 -- Date: Mon, 27 Oct 2014 12:57:41 -0300 3, 19 -- Message-ID: {014001cff1fe$bd500940$37f01bc0$-at-dal.ca} 3, 19 -- MIME-Version: 1.0 3, 19 -- Content-Type: text/plain; 3, 19 -- charset="us-ascii" 3, 19 -- Content-Transfer-Encoding: 7bit 3, 19 -- X-Mailer: Microsoft Outlook 15.0 3, 19 -- Thread-Index: Ac/x/onEE4uZlcUnT0GQ/KZ8GkYgUQ== 3, 19 -- Content-Language: en-us ==============================End of - Headers==============================
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Email: pedro.dacosta-at-kaust.edu.sa Name: Pedro Costa
Organization: KAUST
Title-Subject: [Filtered] KAUST EM Symposium
Message: The King Abdullah University of Science and Technology (KAUST) is organizing its first international symposium dedicated to electron microscopy, scheduled to take place from 9 to 11 December 2014. The event further celebrates the first five years of EM research activities at KAUST as well as progress in the establishment of the EM field in the Middle East.
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Oct 27 18:13:08 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9RND8s0000656 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2014 18:13:08 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id B3387778156 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2014 18:13:08 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id NPeL9bXCewhS for {microscopy-at-microscopy.com} ; 12, 28 -- Mon, 27 Oct 2014 18:12:57 -0500 (CDT) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id 9442D778149 12, 28 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2014 18:12:57 -0500 (CDT) 12, 28 -- Message-ID: {544ED179.5070101-at-microscopy.com} 12, 28 -- Date: Mon, 27 Oct 2014 18:12:57 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:KAUST EM Symposium 12, 28 -- References: {201410271930.s9RJUcdf028197-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201410271930.s9RJUcdf028197-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201410271930.s9RJUcdf028197-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
It's no secret I'm a big, no a huge fan of the Wired website. They have a selection of winning images from the Nikon small world contest. As a Microscopist I'm fascinated both by the nature of the subject and the actual images as art.
John Delly use to tell me photomicrographs should be both beautiful and convey information to be great. These meet those criteria.
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 9, 28 -- From frank_karl-at-ardl.com Wed Oct 29 07:06:33 2014 9, 28 -- Received: from cal1-mh747b.smtproutes.com (cal1-mh747b.smtproutes.com [208.70.89.158]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9TC6XpA002920 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 07:06:33 -0500 9, 28 -- X-Katharion-ID: 1414584369.21667.cal1-mh747 9, 28 -- Received: from exchange2k7.ad.ardl.com ([64.19.31.82]) by 9, 28 -- cal1-mh747.smtproutes.com [(192.69.16.69)] with ESMTP via TCP 9, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 29 Oct 2014 12:06:09 +0000 9, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83]) by 9, 28 -- exchange2k7.ad.ardl.com ([fe80::5833:9255:1958:bb83%12]) with mapi; Wed, 29 9, 28 -- Oct 2014 08:06:08 -0400 9, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 9, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 9, 28 -- {microscopy-at-microscopy.com} 9, 28 -- Date: Wed, 29 Oct 2014 08:06:08 -0400 9, 28 -- Subject: photomicrographs as art 9, 28 -- Thread-Topic: photomicrographs as art 9, 28 -- Thread-Index: Ac/zcLf0vRh+p+H7RO2dI7LdcRKtWg== 9, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150772C4F9F956C-at-exchange2k7.ad.ardl.com} 9, 28 -- Accept-Language: en-US 9, 28 -- Content-Language: en-US 9, 28 -- X-MS-Has-Attach: 9, 28 -- X-MS-TNEF-Correlator: 9, 28 -- acceptlanguage: en-US 9, 28 -- Content-Type: text/plain; charset="us-ascii" 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9TC6XpA002920 ==============================End of - Headers==============================
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Email: guosheng.liu-at-usask.ca Name: Guosheng Liu
Organization: University of Saskatchewan
Title-Subject: [Filtered] Help for rotary microtome repair
Message: Hello Listers,
We inherited 4 ancient (1960's) rotary microtomes (LEITZ 1212 and SARTORIOUS) but none of them works properly. After checkup, I found that all the problems were related to specimen feeding sledge(reset)--either stuck due to long-time idle, or stuck due to the damaged thread (?). When sledge moved to the limit, the user might have put extra force on the wheel hence damaged the threads. My specific questions are: 1) is there any way to loosen the stuck sledge (apply special oil, or organic solvent)? 2) are any special tools required to disassemble or assemble the parts? I found the most screws were too tight to unscrew. Any other considerations when putting things back (like calibrations, alignment)? Thanks in advance.
Guosheng
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Oct 29 08:46:26 2014 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9TDkQoG023601 18, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 08:46:26 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id D7200779580 18, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 08:46:26 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id hRPtLLUM766z for {microscopy-at-microscopy.com} ; 18, 28 -- Wed, 29 Oct 2014 08:46:17 -0500 (CDT) 18, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id 6950D77956E 18, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 08:46:17 -0500 (CDT) 18, 28 -- Message-ID: {5450EFA9.6050801-at-microscopy.com} 18, 28 -- Date: Wed, 29 Oct 2014 08:46:17 -0500 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: viaWWW:Help for rotary microtome repair 18, 28 -- References: {201410281956.s9SJuV3C008188-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201410281956.s9SJuV3C008188-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201410281956.s9SJuV3C008188-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Use light penetrating oil to free the stuck parts - let in soak in for 30 minutes to overnight. And clean like mad. Most of the problem is going to be old dirt and old, stiff lubricants. No special tools are needed, just the usual screwdrivers, wrenches, maybe a pliers, and something to throw in frustration.
If the feed threads are damaged, these can usually be fixed with a thread-chasing tool or a thread tap, if one can found with the right thread pitch.
Phil
} Email: guosheng.liu-at-usask.ca } Name: Guosheng Liu } } Organization: University of Saskatchewan } } Title-Subject: [Filtered] Help for rotary microtome repair } } Message: Hello Listers, } } We inherited 4 ancient (1960's) rotary microtomes (LEITZ 1212 and SARTORIOUS) but none of them works } properly. After checkup, I found that all the problems were related to specimen feeding } sledge(reset)--either stuck due to long-time idle, or stuck due to the damaged thread (?). When } sledge moved to the limit, the user might have put extra force on the wheel hence damaged the threads. } My specific questions are: 1) is there any way to loosen the stuck sledge (apply special oil, or } organic solvent)? 2) are any special tools required to disassemble or assemble the parts? I found } the most screws were too tight to unscrew. Any other considerations when putting things back (like } calibrations, alignment)? } Thanks in advance. } } Guosheng -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 32 -- From oshel1pe-at-cmich.edu Wed Oct 29 09:09:36 2014 5, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9TE9abf011056 5, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 09:09:36 -0500 5, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 5, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id s9TE98BN011452; 5, 32 -- Wed, 29 Oct 2014 10:09:35 -0400 5, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f8c::8dd1:f8c) by 5, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 5, 32 -- (TLS) id 14.3.195.1; Wed, 29 Oct 2014 10:09:25 -0400 5, 32 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 5, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 5, 32 -- 14.3.195.1; Wed, 29 Oct 2014 10:09:25 -0400 5, 32 -- Message-ID: {5450F515.80702-at-cmich.edu} 5, 32 -- Date: Wed, 29 Oct 2014 10:09:25 -0400 5, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 5, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 32 -- MIME-Version: 1.0 5, 32 -- To: micro {Microscopy-at-microscopy.com} , {guosheng.liu-at-usask.ca} 5, 32 -- Subject: Re: [Microscopy] viaWWW:Help for rotary microtome repair 5, 32 -- References: {201410291359.s9TDxRKC008470-at-ns.microscopy.com} 5, 32 -- In-Reply-To: {201410291359.s9TDxRKC008470-at-ns.microscopy.com} 5, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit 5, 32 -- X-Originating-IP: [141.209.160.122] 5, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 32 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 5, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 5, 32 -- X-CanItPRO-Stream: default 5, 32 -- X-Canit-Stats-ID: 02N929zgy - b98a0ddce329 - 20141029 5, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Best known to me commercially-available penetrant for loosing threads and stuck gears on old instruments is "Kroil" from Kano Labs, but in the pinch automotive break fluid and/or kerosene in combination with WD-40 would also do wonders. Wet generously and leave soaking overnight.
Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
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does anybody know the name and function of the nodule-like structures on the bottom side of this sweet potato leaf: http://www.electronmicroscopy.info/sweet_potato_leaf.jpg (3MB) See detail in red circle. There might also be some hyphen present...
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: SciTech-at-wwu.edu Name: Erin Macri
Organization: Western Washington University
Title-Subject: [Filtered] CPD-Liquid CO2 Consumption w/ E3000
Message: We have a Polaron E3000 series CPD and have been having ongoing trouble with liquid CO2 delivery to the chamber. Specifically, we have a great degree of variability in the number (1-8) of CPD cycles we get from a full 50 lb cylinder with siphon tube. We do very little CPD work (2-3 users/year processing 50-100 samples) so I have had a difficult time determining the number of cycles to expect from a cylinder. I chill the chamber to 10 C (with a recirculating chiller unit) before starting and always do a test fill to check for leaks and liquid delivery before putting samples in the chamber. We also weigh the cylinders to track usage and find that we still have 20 lb or more left in the cylinder when it stops delivering liquid. I am aware that a full bottle contains only ~42-43 lb of liquid and that the % liquid in the bottle decreases with increased room temperature. Our lab is in the low to mid 70Â’s year-round. Just today my back-up cylinder stopped delivering liquid after just one CPD cycle and my client lost 12 samples. We feel there may be a supplier issue. We do tank exchanges so have a different cylinder each delivery.
My questions are:
How many cycles (initial fill and two flushes) do other users of this style CPD typically get from a 50 lb siphon bottle? How much is left in the bottle once it no longer delivers liquid C02? Has anyone experienced bad cylinders?
Thank you for your input!
Erin Macri Instrument Center Services Coordinator Scientific Technical Services Western Washington University Bellingham, WA 98225 www.wwu.edu/scitech/
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Oct 29 17:55:26 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9TMtQN7020869 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 17:55:26 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id A3316779A71 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 17:55:26 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id wK4nPkzSswuV for {microscopy-at-microscopy.com} ; 16, 28 -- Wed, 29 Oct 2014 17:55:18 -0500 (CDT) 16, 28 -- Received: from anlvpn001.nst.anl.gov (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 5B413779A64 16, 28 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 17:55:18 -0500 (CDT) 16, 28 -- Message-ID: {54517056.1040305-at-microscopy.com} 16, 28 -- Date: Wed, 29 Oct 2014 17:55:18 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW: 16, 28 -- References: {201410291751.s9THpRIA015446-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201410291751.s9THpRIA015446-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201410291751.s9THpRIA015446-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 16, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We've developed an unusual issue with our venerable (i.e., old) Noran Vantage EDS system. We are seeing an increase in background signal and a loss of peak intensity. The effect is especially pronounced in the low energy (mostly {2kV) portion of the spectrum where the background can be as much as 10 times its normal level. I can send you a file documenting the problem if you contact me directly.
We have also found that turning on the chamber scope and its IR illuminator seems to help temporarily restore functionality. First, the IR floods the detector with photons and the background control software complains and says it shuts off the bias to the detector. Once we turn off the IR and the control software recovers, we have maybe tens of seconds of fairly normal operation before the background problem gradually returns.
Is anyone familiar with this problem before? I've been using EDS since the late 1970s and have not seen this issue before.
Do you have any idea what to do about it - short of sending the detector in for evaluation?
Where may we find the bias value and/or setting within the Vantage software?
Thanks in advance.
Warren Straszheim (wearing my hat as Ames Lab's EPMA operator)
==============================Original Headers============================== 8, 31 -- From wesaia-at-iastate.edu Thu Oct 30 10:07:04 2014 8, 31 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2on0090.outbound.protection.outlook.com [207.46.100.90]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9UF73cB006015 8, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Oct 2014 10:07:04 -0500 8, 31 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by 8, 31 -- BLUPR04MB548.namprd04.prod.outlook.com (10.141.29.152) with Microsoft SMTP 8, 31 -- Server (TLS) id 15.1.6.9; Thu, 30 Oct 2014 15:07:01 +0000 8, 31 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.240]) by 8, 31 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.137]) with mapi id 8, 31 -- 15.01.0006.000; Thu, 30 Oct 2014 15:07:00 +0000 8, 31 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 8, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 31 -- Subject: Noran EDS problems 8, 31 -- Thread-Topic: Noran EDS problems 8, 31 -- Thread-Index: Ac/0UH1Mb4npZcgiSgCGc+EkSY+ULw== 8, 31 -- Date: Thu, 30 Oct 2014 15:07:00 +0000 8, 31 -- Message-ID: {b341888b9f714e1b84c86bbf15141b40-at-BLUPR04MB546.namprd04.prod.outlook.com} 8, 31 -- Accept-Language: en-US 8, 31 -- Content-Language: en-US 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- x-ms-exchange-transport-fromentityheader: Hosted 8, 31 -- x-originating-ip: [147.155.84.39] 8, 31 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID:;SRVR:BLUPR04MB548; 8, 31 -- x-forefront-prvs: 038002787A 8, 31 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(199003)(51874003)(189002)(2501002)(33646002)(40100003)(77096002)(89122001)(76482002)(76576001)(88552001)(64706001)(21056001)(95666004)(101416001)(106356001)(108616004)(107886001)(105586002)(97736003)(85306004)(99286002)(110136001)(229853001)(2351001)(4396001)(122556002)(107046002)(2656002)(90282001)(20776003)(87936001)(92566001)(86362001)(66066001)(120916001)(85852003)(80022003)(54356999)(46102003)(74316001)(50986999)(31966008)(75432002)(24736002);DIR:OUT;SFP:1101;SCL:1;SRVR:BLUPR04MB548;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 8, 31 -- Content-Type: text/plain; charset="us-ascii" 8, 31 -- MIME-Version: 1.0 8, 31 -- X-OriginatorOrg: iastate.edu 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9UF73cB006015 ==============================End of - Headers==============================
With the old liquid nitrogen cooled detectors a number of problems could cause a drop off in low energy peak heights.
1) A degradation of the detector vacuum. 2) Icing up of the detector face due to holes in the window, also contributing to the above. 3) A dirty window.
Others may know better, but my first step would be to disconnect the detector from the rest of the system, and allow it to warn up. This would remove any ice from the detector face. When cooling the detector back down, and then running the system up again, if it takes a long time that would indicate the vacuum problem. Most service technicians will have a device that would allow a vacuum pump to interface with the detector.
Hope this helps?
Steve
Steve Chapman FRMS Protrain for Consultancy and Courses in Electron Microscopy Mobile +44 (0) 7711 606967 Fax +44 (0)1280 814007 www.emcourses.com
X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: 30 October 2014 15:09 To: protrain-at-emcourses.com
X-from: joseph.n.madary.civ-at-mail.mil ()
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Email: joseph.n.madary.civ-at-mail.mil Name: NIck Madary
Organization: joint pathology center
Title-Subject: [Filtered] photo printer sugestions for TEM work?
Message: Hi all, We are using a Gatan Orius CCD attached to a JEOL 1400. We do mostly biologicals and need the occasional high res print. We have not been able to get the best prints from what we have now and would like to purchase something that just gives us a nice photo print without any frills. No need for scanning, faxing, etc.
Does anyone have a newer printer they use, black and white they can recommend? Online is almost too much info to decipher and I am tired of looking. I will now ask the experts! Thanks so much!
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Operation manual for sputter coater and CPD.
Message: Hi, I would be great if you can send me user manual for 1. Sputter coater - Denton Vacuum Desk II 2. Critical Point Dryer - Samdri 790 B Tousimis
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Email: antoniom-at-ictan.csic.es Name: Antonio D. Molina-García
Organization: Instituto de Ciencia y Tecnología de Alimentos y Nutrición-ICTAN (CSIC), Madrid, Spain
Title-Subject: [Filtered] Electronic boards for a Zeiss DSM 960 SEM
Message: Dear all,
We need some electronic boards for a Zeiss DSM 960 SEM. The equipment is old and we cannot find any spares in Spain. We are looking for a “Video Mixer TV Generator 348332-9031 board”, a “Video Switch 348332-9111 board” and a “BAS-interf 348332-9038 board”. The instrument is in a public research centre in Madrid. We would be very grateful if someone can give as some clue of how to find them.”
Best regards,
Antonio D. Molina-García Instituto de Ciencia y Tecnología de Alimentos y Nutrición-ICTAN (CSIC) José Antonio Nováis, 10 Ciudad Universitaria 28040 Madrid SPAIN
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Oct 30 20:40:16 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9V1eGfd032242 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 30 Oct 2014 20:40:16 -0500 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id 57E7477A885 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 30 Oct 2014 20:40:16 -0500 (CDT) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id h-JXDEbhJZep for {microscopy-at-microscopy.com} ; 16, 28 -- Thu, 30 Oct 2014 20:40:12 -0500 (CDT) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id D4D1F77A87A 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 30 Oct 2014 20:40:12 -0500 (CDT) 16, 28 -- Message-ID: {5452E87C.2020709-at-microscopy.com} 16, 28 -- Date: Thu, 30 Oct 2014 20:40:12 -0500 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:Electronic boards for a Zeiss DSM 960 SEM 16, 28 -- References: {201410302353.s9UNr9bT025140-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201410302353.s9UNr9bT025140-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201410302353.s9UNr9bT025140-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hello Warren, We had similar problem in our old EDAX DX4 system on Philips CM12/STEM more then 10 years ago. I've got an advice through this listserver from Matthew Ervin, which solve the problem. I fish out the original message from Nestor's archive for you: -------------------------------------------
Dear colleagues,
We have a position for an application engineer in electron microscopy in Frankfurt.
Details are below. For further information please contact me: achilleas.frangakis-at-biophysik.org
Best wishes,
Achilleas
The electron microscopy group at the Goethe University Frankfurt is seeking an application engineer in electron microscopy.
The job responsibilities include:
• Management and operation of the electron microscopes
• Recording of data for transmission and scanning electron microscopy.
• Training new users and providing support for the existing ones in using the EMs and the different software packages available.
• Trouble-shooting together with the FEI service engineers and Gatan support staff to assure minimal downtimes and optimal performance of the systems.
The position will be one of the staff positions in the electron microscopy group.
The successful applicant should have a solid background in the operation of latest generation electron microscopes and have a good understanding of high resolution imaging demands.
The applicant should be an enthusiastic and capable individual with excellent communication skills and committed to understanding and serving the user community. --
Dr. Anja Seybert Buchmann Institute for Molecular Life Sciences (BMLS) Goethe University Frankfurt Electron Microscopy Frangakis Gruppe, EG 603, 1OG 1.658 Max-von-Laue-Str. 15 60438 Frankfurt am Main Germany
Good news to hear that there are still some other Vantages working somewere ! Our one is still working too, and I'm not very in hurry to switch to a newer system, in spite of the real necessity for a better detector.
I agree with Steve and Oldrich in their diagostic. We had 2 years ago a similar problem, but with a very high deadtime (up to 80-90%) and a high zero peak (PHA status/diagnostic) and no increase of the background itself. And the same effect of the IR light, which reset for a short time to better working conditons.
I warm up the detector, pump it down and bake it fresh (with the help from someone of the list, which lend me the pumping coupling device). Since it works well again. It has a noticable lost in resolution at low energy but a correct sensitivity and no noise and deadtime any more.
The re-pumping and baking themself are not difficult if your are familar with HV and UHV technology and if you have a turbo pump avaible. One must just find the connecting device to open/close the pumping port and pump down.
If your detector is 8-10 or more years old, and you warm it only up, you may have the same problem again in one or two years. This was our case. The windows is always a little bit porous and some air and water wapor goes in the insulation vacuum with time. After 10 years the zeolites are saturated. The warming up will remove the ice, but the zeolites will saturate again very fast. A new pumping down with the baking of the zeolites and all the internal surfaces of the cryostat (much much square meters, with the super insulation film layers) is the best solution.
Hope it helps
Jacques
Le 30/10/2014 16:21, wesaia-at-iastate.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We've developed an unusual issue with our venerable (i.e., old) Noran Vantage EDS system. We are seeing an increase in background signal and a loss of peak intensity. The effect is especially pronounced in the low energy (mostly {2kV) portion of the spectrum where the background can be as much as 10 times its normal level. I can send you a file documenting the problem if you contact me directly. } } We have also found that turning on the chamber scope and its IR illuminator seems to help temporarily restore functionality. First, the IR floods the detector with photons and the background control software complains and says it shuts off the bias to the detector. Once we turn off the IR and the control software recovers, we have maybe tens of seconds of fairly normal operation before the background problem gradually returns. } } Is anyone familiar with this problem before? I've been using EDS since the late 1970s and have not seen this issue before. } } Do you have any idea what to do about it - short of sending the detector in for evaluation? } } Where may we find the bias value and/or setting within the Vantage software? } } Thanks in advance. } } Warren Straszheim (wearing my hat as Ames Lab's EPMA operator) } } } ==============================Original Headers============================== } 8, 31 -- From wesaia-at-iastate.edu Thu Oct 30 10:07:04 2014 } 8, 31 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2on0090.outbound.protection.outlook.com [207.46.100.90]) } 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9UF73cB006015 } 8, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Oct 2014 10:07:04 -0500 } 8, 31 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com (10.141.29.141) by } 8, 31 -- BLUPR04MB548.namprd04.prod.outlook.com (10.141.29.152) with Microsoft SMTP } 8, 31 -- Server (TLS) id 15.1.6.9; Thu, 30 Oct 2014 15:07:01 +0000 } 8, 31 -- Received: from BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.240]) by } 8, 31 -- BLUPR04MB546.namprd04.prod.outlook.com ([169.254.2.137]) with mapi id } 8, 31 -- 15.01.0006.000; Thu, 30 Oct 2014 15:07:00 +0000 } 8, 31 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} } 8, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 8, 31 -- Subject: Noran EDS problems } 8, 31 -- Thread-Topic: Noran EDS problems } 8, 31 -- Thread-Index: Ac/0UH1Mb4npZcgiSgCGc+EkSY+ULw== } 8, 31 -- Date: Thu, 30 Oct 2014 15:07:00 +0000 } 8, 31 -- Message-ID: {b341888b9f714e1b84c86bbf15141b40-at-BLUPR04MB546.namprd04.prod.outlook.com} } 8, 31 -- Accept-Language: en-US } 8, 31 -- Content-Language: en-US } 8, 31 -- X-MS-Has-Attach: } 8, 31 -- X-MS-TNEF-Correlator: } 8, 31 -- x-ms-exchange-transport-fromentityheader: Hosted } 8, 31 -- x-originating-ip: [147.155.84.39] } 8, 31 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID:;SRVR:BLUPR04MB548; } 8, 31 -- x-forefront-prvs: 038002787A } 8, 31 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(199003)(51874003)(189002)(2501002)(33646002)(40100003)(77096002)(89122001)(76482002)(76576001)(88552001)(64706001)(21056001)(95666004)(101416001)(106356001)(108616004)(107886001)(105586002)(97736003)(85306004)(99286002)(110136001)(229853001)(2351001)(4396001)(122556002)(107046002)(2656002)(90282001)(20776003)(87936001)(92566001)(86362001)(66066001)(120916001)(85852003)(80022003)(54356999)(46102003)(74316001)(50986999)(31966008)(75432002)(24736002);DIR:OUT;SFP:1101;SCL:1;SRVR:BLUPR04MB548;H:BLUPR04MB546.namprd04.prod.outlook.com;FPR:;MLV:sfv;PTR:InfoNoRecords;A:1;MX:1;LANG:en; } 8, 31 -- Content-Type: text/plain; charset="us-ascii" } 8, 31 -- MIME-Version: 1.0 } 8, 31 -- X-OriginatorOrg: iastate.edu } 8, 31 -- Content-Transfer-Encoding: 8bit } 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id s9UF73cB006015 } ==============================End of - Headers==============================
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
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Email: a.helvoort-at-ntnu.no Name: Ton van Helvoort
Organization: Norwegian University of Science and Technology (NTNU)
Title-Subject: [Filtered] Open PhD position FIB and TEM of semiconductor nanowire devices
Message: A PhD research fellowship is offered within the project “Low Cost, Ultra-High Efficiency Graphene/Nanowire Solar Cells”. The task of the announced PhD is to characterize the electronic properties, structure and composition of the nanowire-devices by focused ion beam (FIB) and advance transmission electron microscopy (TEM). More details regarding the position and the application procedure can be found on: http://www.ntnu.edu/geminicentre/tem/vacancies
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==============================Original Headers============================== 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Oct 31 17:32:30 2014 12, 28 -- Received: from znl.com ([206.69.208.20]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9VMWT9I030848 12, 28 -- for {microscopy-at-microscopy.com} ; Fri, 31 Oct 2014 17:32:30 -0500 12, 28 -- Received: from localhost (localhost [127.0.0.1]) 12, 28 -- by znl.com (Postfix) with ESMTP id CDC7177B26C 12, 28 -- for {microscopy-at-microscopy.com} ; Fri, 31 Oct 2014 17:32:29 -0500 (CDT) 12, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 28 -- Received: from znl.com ([127.0.0.1]) 12, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id YO2rU1pjBYqF for {microscopy-at-microscopy.com} ; 12, 28 -- Fri, 31 Oct 2014 17:32:22 -0500 (CDT) 12, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 12, 28 -- by znl.com (Postfix) with ESMTPA id B8FCA77B25F 12, 28 -- for {microscopy-at-microscopy.com} ; Fri, 31 Oct 2014 17:32:22 -0500 (CDT) 12, 28 -- Message-ID: {54540DF6.2050700-at-microscopy.com} 12, 28 -- Date: Fri, 31 Oct 2014 17:32:22 -0500 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 12, 28 -- MIME-Version: 1.0 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 28 -- Subject: viaWWW:Open PhD position FIB and TEM of semiconductor nanowire devices 12, 28 -- References: {201410311458.s9VEwA97023642-at-ns.microscopy.com} 12, 28 -- In-Reply-To: {201410311458.s9VEwA97023642-at-ns.microscopy.com} 12, 28 -- X-Forwarded-Message-Id: {201410311458.s9VEwA97023642-at-ns.microscopy.com} 12, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 12, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Those could be glandular trichomes. I could not find an example picture in the same species after a quick internet search, but here is a paper with some of them in Ipomoea cairica :
Paiva Elder Antônio Sousa, Martins Luiza Coutinho (2011) Calycinal trichomes in Ipomoea cairica (Convolvulaceae): ontogenesis, structure and functional aspects. Australian Journal of Botany 59, 91–98.
http://dx.doi.org/10.1071/BT10194
Cheers,
Rafael Buono
On Wed, Oct 29, 2014 at 11:11 AM, {stefan.diller-at-t-online.de} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } does anybody know the name and function of the nodule-like structures on the bottom side of this sweet potato leaf: } http://www.electronmicroscopy.info/sweet_potato_leaf.jpg (3MB) } See detail in red circle. } There might also be some hyphen present... } } } Thanks, } Stefan } } } -- } } } ----------------------------------------------------- } Stefan Diller - Scientific Photography } Arndtstrasse 22 } D - 97072 Wuerzburg Germany } ++49-931-7848700 Phone } ++49-931-7848701 Fax } ++49-175-7177051 Mobile } } Websites: } www.nanoflight.info } www.stefan-diller.com } www.electronmicroscopy.info } www.elektronenmikroskopie.info } www.zwillingsprojekt.de } www.assisi.de } Anfahrt: http://Mail.map24.com/Stefan.Diller } ----------------------------------------------------- } } ==============================Original Headers============================== } 9, 21 -- From stefan.diller-at-t-online.de Wed Oct 29 11:00:38 2014 } 9, 21 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18]) } 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id s9TG0b77019061 } 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 11:00:38 -0500 } 9, 21 -- Received: from fwd06.aul.t-online.de (fwd06.aul.t-online.de [172.20.26.150]) } 9, 21 -- by mailout04.t-online.de (Postfix) with SMTP id 4B76E2A43AF } 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2014 17:00:36 +0100 (CET) } 9, 21 -- Received: from mac-pro.speedport.ip (JT5gv-ZeQhKT610BDBm-1RRuOKpdKnnw1cqrAqBGeRRC83sUNDAxVHPWkuLrXR5QBN-at-[91.58.175.180]) by fwd06.t-online.de } 9, 21 -- with (TLSv1:ECDHE-RSA-AES256-SHA encrypted) } 9, 21 -- esmtp id 1XjVfo-03TrOK0; Wed, 29 Oct 2014 17:00:36 +0100 } 9, 21 -- Message-ID: {54510F23.6040804-at-t-online.de} } 9, 21 -- Date: Wed, 29 Oct 2014 17:00:35 +0100 } 9, 21 -- From: Stefan Diller {stefan.diller-at-t-online.de} } 9, 21 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 } 9, 21 -- MIME-Version: 1.0 } 9, 21 -- To: microscopy-at-microscopy.com } 9, 21 -- Subject: nodule-like structure on sweet potato leaf } 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 9, 21 -- Content-Transfer-Encoding: 7bit } 9, 21 -- X-ID: JT5gv-ZeQhKT610BDBm-1RRuOKpdKnnw1cqrAqBGeRRC83sUNDAxVHPWkuLrXR5QBN } 9, 21 -- X-TOI-MSGID: c9fc6968-b5f5-433d-a86c-cfbe27ad186d } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 31 -- From rafaelbuono-at-gmail.com Sun Nov 2 13:20:36 2014 8, 31 -- Received: from mail-yh0-f44.google.com (mail-yh0-f44.google.com [209.85.213.44]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA2JKapM004031 8, 31 -- for {Microscopy-at-microscopy.com} ; Sun, 2 Nov 2014 13:20:36 -0600 8, 31 -- Received: by mail-yh0-f44.google.com with SMTP id i57so559684yha.31 8, 31 -- for {Microscopy-at-microscopy.com} ; Sun, 02 Nov 2014 11:20:36 -0800 (PST) 8, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 31 -- d=gmail.com; s=20120113; 8, 31 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 8, 31 -- :content-type:content-transfer-encoding; 8, 31 -- bh=00dL7o0PcdA9nyjs8eL1G5jmQLzpmUF9RgE0NL3bzjE=; 8, 31 -- b=UCNJEQVkT9O+axkbMfo89PpOX78ct8mIohlWEm2jxDdk3STYg11mSMNXVFi+kXcWPy 8, 31 -- 7W/oSeQTPMQHIxHtxX4/yB26BtINVwJzc78X6gXTMEdrzOPr0aLTzcTfxk1gGJJ3KMnQ 8, 31 -- Dj8SJpUZcSziXEudSVxW1yVBo8N3+auEbdoioqBEhv4ZVh3JX37ru/2rQjJplKNAJuED 8, 31 -- uUYFSbi5sgAP4w7F/6mLwIu4jDwDYRjeJ+rMl8ndCfl2yh/UReq9urYlkHlJG0Av+v9I 8, 31 -- 4oHdjTdSbuUmAvpg2iCYiEbyaj77Zf932sHjvSIhRS5d76oqfI49qSJKFh+VVrityStC 8, 31 -- 8Q7A== 8, 31 -- MIME-Version: 1.0 8, 31 -- X-Received: by 10.236.190.5 with SMTP id d5mr24427013yhn.120.1414956036208; 8, 31 -- Sun, 02 Nov 2014 11:20:36 -0800 (PST) 8, 31 -- Received: by 10.170.211.66 with HTTP; Sun, 2 Nov 2014 11:20:36 -0800 (PST) 8, 31 -- In-Reply-To: {201410291611.s9TGB1C1002162-at-ns.microscopy.com} 8, 31 -- References: {201410291611.s9TGB1C1002162-at-ns.microscopy.com} 8, 31 -- Date: Sun, 2 Nov 2014 13:20:36 -0600 8, 31 -- Message-ID: {CANHQk2Y80fd0hu0hgBfO=AcPLsn0T+gvfSLzLf9P40PqQVoP=g-at-mail.gmail.com} 8, 31 -- Subject: Re: [Microscopy] nodule-like structure on sweet potato leaf 8, 31 -- From: rafael buono {rafaelbuono-at-gmail.com} 8, 31 -- To: Microscopy-at-microscopy.com 8, 31 -- Content-Type: text/plain; charset=UTF-8 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sA2JKapM004031 ==============================End of - Headers==============================
We picked up this random leaf from the lawn and saw these "budding" structures on the leaf surface when doing cryoSEM on it. Does anyone have some idea what are these structures called on a leaf? Sorry, we don't know what kind of leaf it is when we pick it up.
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Email: michelle.gignac-at-duke.edu Name: Michelle
Organization: Duke University
Title-Subject: [Filtered] Embedding Qustion
Message: Hello All.
I have a PI that is interested in immuno-labeling cells grown in 6 well plates. He would like to keep the cells in the plates during dehydration and embedding to keep the cell-cell interactions intact. The antigen he is interested in requires post embedding labeling. My question is what resin can I use that will not interact with the plastic plates? Normally I would use the Polybed 812 resin, but will this work with immuno-labeling?
Thanks for your help. Michelle
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==============================Original Headers============================== 17, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Nov 3 21:31:41 2014 17, 28 -- Received: from znl.com ([206.69.208.20]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA43Vfdk032366 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Nov 2014 21:31:41 -0600 17, 28 -- Received: from localhost (localhost [127.0.0.1]) 17, 28 -- by znl.com (Postfix) with ESMTP id E483A77D855 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Nov 2014 21:31:41 -0600 (CST) 17, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 17, 28 -- Received: from znl.com ([127.0.0.1]) 17, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id pO9X5GFCfMNP for {microscopy-at-microscopy.com} ; 17, 28 -- Mon, 3 Nov 2014 21:31:34 -0600 (CST) 17, 28 -- Received: from anlvpn001.nst.anl.gov (mac22.zaluzec.com [206.69.208.22]) 17, 28 -- by znl.com (Postfix) with ESMTPA id 6F52077D847 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Nov 2014 21:31:34 -0600 (CST) 17, 28 -- Message-ID: {54584896.6080105-at-microscopy.com} 17, 28 -- Date: Mon, 03 Nov 2014 21:31:34 -0600 17, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 17, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 17, 28 -- MIME-Version: 1.0 17, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 28 -- Subject: viaWWW:Embedding Qustion 17, 28 -- References: {201411032054.sA3KsxsU028485-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201411032054.sA3KsxsU028485-at-ns.microscopy.com} 17, 28 -- X-Forwarded-Message-Id: {201411032054.sA3KsxsU028485-at-ns.microscopy.com} 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
It would be helpful if you can provide more information on the protein your PI is trying to label and why he thinks the antigen requires post embedding labeling. With all due respect to the users of our core, my experience is most of them are not knowledgeable enough for immunoelectron microscopy so although I listen to their opinion, I do have to make my own decision on how to pursue to labeling. The important thing is to ask a lot questions and to thoroughly understand the system/project. Feel free to contact me off line if you want to discuss further. Thanks.
Hong hyi-at-emory.edu
On 11/3/14 10:35 PM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
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==============================Original Headers============================== 14, 40 -- From hyi-at-emory.edu Mon Nov 3 23:22:15 2014 14, 40 -- Received: from ws-mr1.cc.emory.edu (ws-mr1.cc.emory.edu [170.140.50.231]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA45MFm6023146 14, 40 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2014 23:22:15 -0600 14, 40 -- Received: from e14edge1n.Emory.Edu (e14edge1n.eu.emory.edu [170.140.52.18] (may be forged)) 14, 40 -- by ws-mr1.cc.emory.edu (8.13.8/8.13.8) with ESMTP id sA41KQgI012898 14, 40 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2014 20:20:26 -0500 14, 40 -- Received: from E14CH2N.Enterprise.emory.net (10.240.8.114) by 14, 40 -- e14edge1n.Emory.Edu (170.140.52.18) with Microsoft SMTP Server (TLS) id 14, 40 -- 14.3.210.2; Tue, 4 Nov 2014 00:22:13 -0500 14, 40 -- Received: from E14MBX14N.Enterprise.emory.net ([fe80::e464:5551:d968:fbcc]) by 14, 40 -- e14ch2n.Enterprise.emory.net ([::1]) with mapi id 14.03.0174.001; Tue, 4 Nov 14, 40 -- 2014 00:22:12 -0500 14, 40 -- From: "Yi, Hong" {hyi-at-emory.edu} 14, 40 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 14, 40 -- Subject: Re: [Microscopy] viaWWW:Embedding Qustion 14, 40 -- Thread-Topic: [Microscopy] viaWWW:Embedding Qustion 14, 40 -- Thread-Index: AQHP9+BfXJ+wTO1wdE60ud2p6JRgLJxP7uoA 14, 40 -- Date: Tue, 4 Nov 2014 05:22:12 +0000 14, 40 -- Message-ID: {D07DC4A6.1230C%hyi-at-emory.edu} 14, 40 -- References: {201411040335.sA43ZMb4003138-at-ns.microscopy.com} 14, 40 -- In-Reply-To: {201411040335.sA43ZMb4003138-at-ns.microscopy.com} 14, 40 -- Accept-Language: en-US 14, 40 -- Content-Language: en-US 14, 40 -- X-MS-Has-Attach: 14, 40 -- X-MS-TNEF-Correlator: 14, 40 -- user-agent: Microsoft-MacOutlook/14.4.5.141003 14, 40 -- x-originating-ip: [170.140.30.131] 14, 40 -- Content-Type: text/plain; charset="us-ascii" 14, 40 -- Content-ID: {21AD317F3B594E4E99E8552201476217-at-Enterprise.emory.net} 14, 40 -- MIME-Version: 1.0 14, 40 -- X-Emory-MailScanner-Information: Please contact the ISP for more information 14, 40 -- X-Emory-MailScanner-ID: sA41KQgI012898 14, 40 -- X-Emory-MailScanner: Found to be clean 14, 40 -- X-Emory-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 14, 40 -- score=-0.594, required 8, autolearn=disabled, RP_MATCHES_RCVD -0.59) 14, 40 -- X-Emory-MailScanner-From: hyi-at-emory.edu 14, 40 -- X-Spam-Status: No 14, 40 -- Content-Transfer-Encoding: 8bit 14, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sA45MFm6023146 ==============================End of - Headers==============================
these are multicellular trichomes that are quite common for plants.
Best regards, Anne Heller
----- Nachricht von xuanhao.sun-at-uconn.edu --------- Datum: Mon, 3 Nov 2014 16:04:47 -0600 Von: xuanhao.sun-at-uconn.edu Antwort an: xuanhao.sun-at-uconn.edu Betreff: [Microscopy] Help to identify a structure on leaf surface An: Anne.Heller-at-uni-hohenheim.de
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } We picked up this random leaf from the lawn and saw these "budding" } structures on the leaf surface when doing cryoSEM on it. Does anyone } have some idea what are these structures called on a leaf? Sorry, we } don't know what kind of leaf it is when we pick it up. } } http://emlab.uconn.edu/wp-content/uploads/sites/370/2014/11/Leaf-Surface.jpg } } Xuanhao Sun, Ph.D. } } Bioscience Electron Microscopy Laboratory } University of Connecticut } BPB G06, Unit 3242 } Storrs, CT 06269-3242 } } Office Tel: 860-486-6368 } Lab Tel: 860-486-2914 } Fax: 860-486-6369 } website: emlab.uconn.edu } } } } } ==============================Original Headers============================== } 7, 24 -- From xuanhao.sun-at-uconn.edu Mon Nov 3 15:45:56 2014 } 7, 24 -- Received: from exchange.uconn.edu } (cashubc.grove.ad.uconn.edu [137.99.30.168]) } 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id sA3LjtMa030407 } 7, 24 -- for {Microscopy-at-Microscopy.com} ; Mon, 3 Nov 2014 15:45:56 -0600 } 7, 24 -- Received: from MAILB.grove.ad.uconn.edu ([169.254.3.150]) by } 7, 24 -- CASHUBC.grove.ad.uconn.edu ([137.99.30.168]) with mapi id } 14.03.0210.002; } 7, 24 -- Mon, 3 Nov 2014 16:45:55 -0500 } 7, 24 -- From: "Sun, Xuanhao" {xuanhao.sun-at-uconn.edu} } 7, 24 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} } 7, 24 -- Subject: Help to identify a structure on leaf surface } 7, 24 -- Thread-Topic: Help to identify a structure on leaf surface } 7, 24 -- Thread-Index: AQHP96+LB2nBw+L5eUy9LhDp+/OeJg== } 7, 24 -- Date: Mon, 3 Nov 2014 21:45:54 +0000 } 7, 24 -- Message-ID: {EA9DDA1F-C2B8-4925-8641-E918DBA4D1A4-at-uconn.edu} } 7, 24 -- Accept-Language: en-US } 7, 24 -- Content-Language: en-US } 7, 24 -- X-MS-Has-Attach: } 7, 24 -- X-MS-TNEF-Correlator: } 7, 24 -- x-originating-ip: [137.99.47.47] } 7, 24 -- Content-Type: text/plain; charset="us-ascii" } 7, 24 -- Content-ID: {349E32447A6399479C1856ADC5A86545-at-ad.uconn.edu} } 7, 24 -- MIME-Version: 1.0 } 7, 24 -- Content-Transfer-Encoding: 8bit } 7, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id sA3LjtMa030407 } ==============================End of - Headers==============================
----- Ende der Nachricht von xuanhao.sun-at-uconn.edu -----
Dr. Anne Heller AG Elektronenmikroskopie Institut für Botanik (210) Universität Hohenheim Postfach, 70593 Stuttgart Tel. 0711-45922180 Fax. 0711-45923355
==============================Original Headers============================== 12, 32 -- From Anne.Heller-at-uni-hohenheim.de Tue Nov 4 01:31:25 2014 12, 32 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA47VOUE015194 12, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Nov 2014 01:31:24 -0600 12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/simple; d=uni-hohenheim.de; 12, 32 -- h=content-transfer-encoding:content-disposition:mime-version 12, 32 -- :content-type:content-type:user-agent:in-reply-to:references 12, 32 -- :subject:subject:from:from:message-id:date:date; s=main; t= 12, 32 -- 1415086281; x=1416900682; bh=SXlDmlMqjIPO05pwVCO7lSpnIf6RAcE1+I2 12, 32 -- H7GCM9tY=; b=BCwHDIznLp28nNZfZ+mnooH5YwM9Hvbg7n0sNk+LzbK49oOUSSE 12, 32 -- +yhP99LR89L96H0v07XaR2nhsTceacpTLPcpSmbCjD8iYLV+hEjZSkr9T3dWmrFN 12, 32 -- 9wyGM6e6+wYMHut2sqfoShFkxemxWyPZ+R/GOzL9eg/55jtK/zPqzId4= 12, 32 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 12, 32 -- Received: from webmail.rz.uni-hohenheim.de (mailbox3.rz.uni-hohenheim.de [144.41.4.59]) 12, 32 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP; 12, 32 -- Tue, 4 Nov 2014 08:31:20 +0100 (CET) 12, 32 -- Received: from jatropha.botanik.uni-hohenheim.de 12, 32 -- (jatropha.botanik.uni-hohenheim.de [144.41.33.113]) by 12, 32 -- webmail.uni-hohenheim.de (Horde Framework) with HTTP; Tue, 04 Nov 2014 12, 32 -- 08:31:20 +0100 12, 32 -- Date: Tue, 04 Nov 2014 08:31:20 +0100 12, 32 -- Message-ID: {20141104083120.Horde.vkrKHhw3VaPqhHeA6vVEOA1-at-webmail.uni-hohenheim.de} 12, 32 -- From: "Dr. Anne Heller" {Anne.Heller-at-uni-hohenheim.de} 12, 32 -- To: Microscopy-at-microscopy.com, xuanhao.sun-at-uconn.edu 12, 32 -- Subject: Re: [Microscopy] Help to identify a structure on leaf surface 12, 32 -- References: {201411032204.sA3M4lww017856-at-ns.microscopy.com} 12, 32 -- In-Reply-To: {201411032204.sA3M4lww017856-at-ns.microscopy.com} 12, 32 -- User-Agent: Internet Messaging Program (IMP) H5 (6.1.4) 12, 32 -- Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes 12, 32 -- MIME-Version: 1.0 12, 32 -- Content-Disposition: inline 12, 32 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
It's been a long time since I've done bio SEM prep, our lab mostly does materials samples. But I've been getting more and more hydrated samples, hydrogels, bacteria, etc. So far we've been air-drying, I do have access to a freeze drier but haven't tried it yet. What is considered state-of the art now for these kinds of preps? Critical point? Cryo-SEM? Fixation?
Thank you, Leslie Thompson IBM Research-Almaden San Jose, CA
==============================Original Headers============================== 3, 26 -- From 2lesliethompson-at-gmail.com Tue Nov 4 17:58:49 2014 3, 26 -- Received: from mail-la0-f45.google.com (mail-la0-f45.google.com [209.85.215.45]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA4Nwnlw027717 3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Nov 2014 17:58:49 -0600 3, 26 -- Received: by mail-la0-f45.google.com with SMTP id pn19so1852413lab.32 3, 26 -- for {microscopy-at-microscopy.com} ; Tue, 04 Nov 2014 15:58:48 -0800 (PST) 3, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=20120113; 3, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 26 -- bh=tqFYVBUr117TrfB106ZAehB3JKcJ6U6EYxClk7HItCQ=; 3, 26 -- b=fiU3aGPDqS6nN5c6Z6w7LMBwmRpywrJFxH6IJWqADbf9fnrGqDpraQkdHBQQWmmEyQ 3, 26 -- tECFevtE0xwpfwmwBCjvXNnoazds4PAOEAde95rhODlG5vhaShtyyPHc2kPwqVi/46BU 3, 26 -- viQ0QEwgQHHz2jaXL4NUBc+t1dzoV+u6+PGQSGGdicuECXmYc8/7bsLkiQ8ba7yIp5bi 3, 26 -- u/lVQdgcUPRdM5PHSe89Czig+ecDH27fORjrP7Wc6+c8teigl3jBtQMgw4m/tyTGlTbi 3, 26 -- 4+aVeOnF1ZDvV7l/VklhJreGF1G8dX82v0YkYBpGK96cHbZzhj4mgOHNcvRn95XVciP6 3, 26 -- aldw== 3, 26 -- MIME-Version: 1.0 3, 26 -- X-Received: by 10.112.239.12 with SMTP id vo12mr63659233lbc.81.1415145528505; 3, 26 -- Tue, 04 Nov 2014 15:58:48 -0800 (PST) 3, 26 -- Received: by 10.25.216.135 with HTTP; Tue, 4 Nov 2014 15:58:48 -0800 (PST) 3, 26 -- Date: Tue, 4 Nov 2014 15:58:48 -0800 3, 26 -- Message-ID: {CAFDDb=PVAOdmCu=Mr+9Ubm65J4kU-5cPZ5sXOUi9wn2ni4jx_Q-at-mail.gmail.com} 3, 26 -- Subject: SEM sample prep for hydrogels? 3, 26 -- From: Leslie Thompson {2lesliethompson-at-gmail.com} 3, 26 -- To: microscopy-at-microscopy.com 3, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Air drying is the worst way to prepare hydrogels. The best way to do SEM of these is cryoSEM: high-pressure freezing or plunge freezing into slush nitrogen (not liquid nitrogen). Plunging into a LN2 cooled cryogen is next best. Then image frozen - just vacuum sublimate the top micrometer or so of the ice. Don't let the frozen gel get above -90 C.
Freeze-drying can work IFF it's done correctly - meaning stopping at about -60 C and about -40 C to remove the structural water (aka "water of collapse" for obvious reasons).
If you can't do either of those, CPD can work, but it will still alter the gel structures.
One problem with CPD is the dehydration steps - those alter the gel structure also. IFF your gels are of a nature that is not affected by EtOH or acetone *and* not by t-butyl alcohol, you might try freeze drying from t-butyl. Much simplier, but I don't know if anyone has actually tried this.
But. Given these are hydrogels and the water is an important part of the structure, cryoSEM is really the best and perhaps only way to image them and get something close to reality.
Phil
} Hello- } } It's been a long time since I've done bio SEM prep, our lab mostly does } materials samples. But I've been getting more and more hydrated samples, } hydrogels, bacteria, etc. So far we've been air-drying, I do have access } to a freeze drier but haven't tried it yet. What is considered state-of } the art now for these kinds of preps? Critical point? Cryo-SEM? Fixation? } } Thank you, } Leslie Thompson } IBM Research-Almaden } San Jose, CA -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 8, 32 -- From oshel1pe-at-cmich.edu Wed Nov 5 06:47:47 2014 8, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA5Cll8e016441 8, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Nov 2014 06:47:47 -0600 8, 32 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 8, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id sA5Clk5T011249; 8, 32 -- Wed, 5 Nov 2014 07:47:46 -0500 8, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f8c::8dd1:f8c) by 8, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 8, 32 -- (TLS) id 14.3.195.1; Wed, 5 Nov 2014 07:47:46 -0500 8, 32 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 8, 32 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 8, 32 -- 14.3.195.1; Wed, 5 Nov 2014 07:47:45 -0500 8, 32 -- Message-ID: {545A1C6B.1080408-at-cmich.edu} 8, 32 -- Date: Wed, 5 Nov 2014 07:47:39 -0500 8, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 8, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 8, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 8, 32 -- MIME-Version: 1.0 8, 32 -- To: {2lesliethompson-at-gmail.com} , micro {Microscopy-at-microscopy.com} 8, 32 -- Subject: Re: [Microscopy] SEM sample prep for hydrogels? 8, 32 -- References: {201411050015.sA50FMAU012566-at-ns.microscopy.com} 8, 32 -- In-Reply-To: {201411050015.sA50FMAU012566-at-ns.microscopy.com} 8, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 8, 32 -- Content-Transfer-Encoding: 7bit 8, 32 -- X-Originating-IP: [141.209.160.122] 8, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 8, 32 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 8, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 8, 32 -- X-CanItPRO-Stream: default 8, 32 -- X-Canit-Stats-ID: 02NbMLKoK - b9f43dffbf07 - 20141105 8, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mmcheath-at-syr.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mmcheath-at-syr.edu Name: Michael Cheatham
Organization: Syracuse University
Title-Subject: [Filtered] Troubleshootong a Gresham-Avalon-Spirit system
Message: My aplogies for using the web form. I am a subscriber to the Microscopy Listserv list but I've never had luck posting directly.
I have just installed a new (to me) Gresham EDS detector (model 107-70, serial # 50798) onto the fifth WDS position (most right hand side position) of a JEOL 8600. I acquired this detector a few years ago from the Univ of Missouri Rolla campus. It was on a JEOL733 in the fourth position. I used the same mounting plate to install it on my 8600. The Gresham is connected to a PGT Avalon Model 8K box. I am using Spirit software version 1.7.5b.
In short, my problem is that I get no counts in the Spirit software.
The computer is a Dell with Windows XP. This system 9Dell/Spirit/Avalon/Gresham) was working in Rolla. Since the computer came from the U of Missouri system I had to wipe it and re-install XP and lost everything.
Might anyone on this list have a Gresham-Avalon-Spirit system? I need a few answers to help with the complete software installation set-up.
I have a picture of the detector serial # plate and the interface to the 8600 column here: http://earthsciences.syr.edu/Other/Gresham-EDS-SU.html
Can anyone help with the black knob at the base of the detector just at the interface plate. It has six positions 0,1,2,3,4,5 abd I believe these select a mechanical aperture that rotates in front of the end of the detector inside the vacuum chamber.
Mike
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Email: rebecca.jackson-at-utsouthwestern.edu Name: Rebecca Jackson
Organization: UT Southwestern
Title-Subject: [Filtered] Digital Water Bath
Message: Morning all! We are looking at purchasing a water bath from Thermo Scientific. We always had an analog one but we were thinking of purchasing a digital one with an internal thermometer. Anyone have one? How long do the internal thermometers last? Any comments...?
Thanks!
-Bec.
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==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Nov 6 23:02:28 2014 13, 28 -- Received: from znl.com ([206.69.208.20]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA752Sg4028259 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 6 Nov 2014 23:02:28 -0600 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by znl.com (Postfix) with ESMTP id 0E46A77FAA0 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 6 Nov 2014 23:02:28 -0600 (CST) 13, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 28 -- Received: from znl.com ([127.0.0.1]) 13, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with ESMTP id n3B+fAKVih0j for {microscopy-at-microscopy.com} ; 13, 28 -- Thu, 6 Nov 2014 23:02:20 -0600 (CST) 13, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 13, 28 -- by znl.com (Postfix) with ESMTPA id EB41877FA93 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 6 Nov 2014 23:02:20 -0600 (CST) 13, 28 -- Message-ID: {545C525C.5060002-at-microscopy.com} 13, 28 -- Date: Thu, 06 Nov 2014 23:02:20 -0600 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:Digital Water Bath 13, 28 -- References: {201411061603.sA6G3DF3024958-at-ns.microscopy.com} 13, 28 -- In-Reply-To: {201411061603.sA6G3DF3024958-at-ns.microscopy.com} 13, 28 -- X-Forwarded-Message-Id: {201411061603.sA6G3DF3024958-at-ns.microscopy.com} 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: DAVID.SMITH-at-asu.edu Name: David Smith
Organization: Arizona State University
Title-Subject: [Filtered] Cluster hires in aberration-corrected electron microscopy
Message: Department of Physics, Arizona State University, Tempe, AZ
The Department of Physics at Arizona State University (ASU) seeks to fill up to three faculty positions in the topical area of aberration-corrected electron microscopy. Two positions will be at the assistant/associate professor level and the third appointment is an open rank position. Rank and tenure status will be commensurate with the candidateÂ’s experience and publication record. Applicants must have a Ph.D. or equivalent in physics or a closely related field at the time of appointment, and demonstrated research experience in the field of aberration-corrected electron microscopy. An outstanding record of accomplishments with clear potential to establish a vigorous research program that will attract external funding, show a strong commitment to excellence in teaching and demonstrated experience in a multidisciplinary collaborative environment are highly desirable. The appointments are expected to begin in August 2015.
We seek applicants who will help to leverage recent substantial investments in facilities and instrumentation by the University for promoting cutting-edge electron microscopy. ASU is home to the John M. Cowley Center for High Resolution Electron Microscopy and has a well-established international reputation in transmission electron microscopy. The university has recently established the Southwestern Center for Aberration Corrected Electron Microscopy, which features three aberration-corrected instruments, also allowing monochromated and in situ microscopy as well as electron holography. The successful applicants are expected to develop strong experimental and/or theoretical programs that will advance the scientific capabilities of these new instruments.
Complete applications will include a cover letter, curriculum vitae, statements of plans for research (3 to 5 pages) and teaching (1 page), and contact information for three references (postal and email addresses, and phone number). Materials must be submitted on-line at http://physics.asu.edu/employment.php. Only electronic applications will be considered.
Review of applications will commence December 1, 2014. Further review of completed files will be made every two weeks until the search is closed. A background check will be required for employment.
Arizona State University is a VEVRAA Federal Contractor and an Equal Opportunity/Affirmative Action Employer. All qualified applicants will be considered without regard to race, color, sex, religion, national origin, disability, protected veteran status, or any other basis protected by law. https://www.asu.edu/aad/manuals/acd/acd401.html https://www.asu.edu/titleIX/
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==============================Original Headers============================== 23, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Nov 6 23:20:10 2014 23, 28 -- Received: from znl.com ([206.69.208.20]) 23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA75KAtE014301 23, 28 -- for {microscopy-at-microscopy.com} ; Thu, 6 Nov 2014 23:20:10 -0600 23, 28 -- Received: from localhost (localhost [127.0.0.1]) 23, 28 -- by znl.com (Postfix) with ESMTP id CE7C977FAEE 23, 28 -- for {microscopy-at-microscopy.com} ; Thu, 6 Nov 2014 23:20:10 -0600 (CST) 23, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 23, 28 -- Received: from znl.com ([127.0.0.1]) 23, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 23, 28 -- with ESMTP id Sn5lk72LrhSD for {microscopy-at-microscopy.com} ; 23, 28 -- Thu, 6 Nov 2014 23:20:01 -0600 (CST) 23, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 23, 28 -- by znl.com (Postfix) with ESMTPA id 199A977FAE1 23, 28 -- for {microscopy-at-microscopy.com} ; Thu, 6 Nov 2014 23:20:01 -0600 (CST) 23, 28 -- Message-ID: {545C5680.3040104-at-microscopy.com} 23, 28 -- Date: Thu, 06 Nov 2014 23:20:00 -0600 23, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 23, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 23, 28 -- MIME-Version: 1.0 23, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 23, 28 -- Subject: viaWWW:Cluster hires in aberration-corrected electron microscopy 23, 28 -- References: {201411070517.sA75HS7D010552-at-ns.microscopy.com} 23, 28 -- In-Reply-To: {201411070517.sA75HS7D010552-at-ns.microscopy.com} 23, 28 -- X-Forwarded-Message-Id: {201411070517.sA75HS7D010552-at-ns.microscopy.com} 23, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed 23, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
South Dakota School of Mines and Technology has posted a job listing at the link below for a faculty position in the Nanoscience and Nanoengineering Ph.D. Program. Applicants should have research interest in advanced, optical bio-imaging methods:
I have a Hitachi S-450 SEM that came with a Kevex EDS unit. The old model 7000 frame that came with it is dead and a friend gave me a "not quite as old” Kevex Sigma Gold MCA to control it. I was able to get service schematics and software for it from Thermo Fisher but I have not been able to scrounge up any manuals other than the basic operation manuals found on the web and on the CD with the software.
Connecting the Sigma to the sensor head does not looks to be a big deal, I just need to supply -24v for the 2003 preamp in place of the -15 used on the 2008 preamp. But where I am getting stuck is on the System Controller module, the schematics I was sent do not include this version of the module. The one on this rack has the two type E thermocouple inputs. What I don’t know what to do with is the DB-15 for “BEAM” and the MAP/SCAN connector. I have no idea how to set any of this up.
My unit also has the DBC card for connecting to the 4855 scope control box and would like to find any setup information on that unit as well, I still need to find one of those boxes, there is one on eBay for an almost reasonable price.
-Jerry
==============================Original Headers============================== 4, 34 -- From jerry.biehler-at-gmail.com Fri Nov 7 14:50:34 2014 4, 34 -- Received: from mail-pd0-f175.google.com (mail-pd0-f175.google.com [209.85.192.175]) 4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA7KoYhZ027803 4, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Nov 2014 14:50:34 -0600 4, 34 -- Received: by mail-pd0-f175.google.com with SMTP id y13so3977675pdi.6 4, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 07 Nov 2014 12:50:34 -0800 (PST) 4, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 34 -- d=gmail.com; s=20120113; 4, 34 -- h=from:content-type:content-transfer-encoding:subject:message-id:date 4, 34 -- :to:mime-version; 4, 34 -- bh=E9Q7gYyKYeyWdP+kf/FIZLMYkG344jeLchfCL4SEiEA=; 4, 34 -- b=TrsIHOxXqlLSU+wyjSon2K+A8BThJsdEitogasWrR+e24DlELWSHk9DPOnrNKIctAI 4, 34 -- MCT8janBn+4ace8wf5P7Y+VTyLqpPyLQVkwcqiQUj4XSFpHf6YII/MDhp12ZXC8acbe2 4, 34 -- kyh/HPORNoqNPUkls8Hdd1cPlSQ9iZJZtZhTkf+Q+sYXK50YePOQ3s48s5yaC2xbmuu1 4, 34 -- k9lxe5hvFXQrti1N38hOVLfZil4931lRQFzX80NEXzdn59+GG3o8ZW4dQdLKOtREN5wQ 4, 34 -- 7/ixV/DXfj/+eAlhkMpIGXuHSA0kzKwmrGwkBO4eZYcHapcXdjos59WF5zsnxPkUu0LM 4, 34 -- 3aYg== 4, 34 -- X-Received: by 10.66.194.39 with SMTP id ht7mr14659247pac.91.1415393433975; 4, 34 -- Fri, 07 Nov 2014 12:50:33 -0800 (PST) 4, 34 -- Received: from [10.0.38.174] (50-196-14-2-static.hfc.comcastbusiness.net. [50.196.14.2]) 4, 34 -- by mx.google.com with ESMTPSA id ql6sm2305959pbb.39.2014.11.07.12.50.32 4, 34 -- for {Microscopy-at-microscopy.com} 4, 34 -- (version=TLSv1 cipher=ECDHE-RSA-RC4-SHA bits=128/128); 4, 34 -- Fri, 07 Nov 2014 12:50:33 -0800 (PST) 4, 34 -- From: Jerry Biehler {jerry.biehler-at-gmail.com} 4, 34 -- Content-Type: text/plain; charset=utf-8 4, 34 -- Subject: Installing a Kevex Sigma and 4855 4, 34 -- Message-Id: {5EC9A0A6-EAB5-4B1E-B714-B7DDEBAFFFAE-at-gmail.com} 4, 34 -- Date: Fri, 7 Nov 2014 12:50:31 -0800 4, 34 -- To: Microscopy-at-microscopy.com 4, 34 -- Mime-Version: 1.0 (Mac OS X Mail 8.0 \(1990.1\)) 4, 34 -- X-Mailer: Apple Mail (2.1990.1) 4, 34 -- Content-Transfer-Encoding: 8bit 4, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sA7KoYhZ027803 ==============================End of - Headers==============================
My colleague and I are interested in compiling a list of all the aberration corrected electron microscopes--SEM and TEM--in the US (only in the US purely due to laziness and not patriotism). We co-teach a course on electron microscopy and want to show how rapidly these advanced instruments have propagated into universities and industry. The early picture of AC-EM is easy to draw--there are just a few data points to plot on our graphic--but the picture today is too much work to compile for a two man team, yet we think it will drive the point home to our students on how important this advancement in electron optics is to the community.
Has anyone compiled such a list in the last year or two? If so, would you be willing to share with us? Full credit will be given to you in our lectures. Assuming such a list does not exist or is not recent, please help us create one by providing the following information:
1) The name of your university or company if you have an aberration corrected electron microscope 2) The number of aberration corrected microscopes in your facility (please note if you have one of the very few AC-SEMs in the US; otherwise S/TEM will be assumed) 3) Your city and state
The results will be overlaid on a map of the US, and we'll freely share with the community.
Thank you for any help you can provide in compiling this list, Chris Senior Research Associate Nanoscale Characterization and Fabrication Laboratory Institute for Critical Technology and Applied Science Virginia Tech (540) 200-9511
==============================Original Headers============================== 6, 26 -- From microwink-at-gmail.com Fri Nov 7 17:45:27 2014 6, 26 -- Received: from mail-yk0-f175.google.com (mail-yk0-f175.google.com [209.85.160.175]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA7NjRLf005015 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Nov 2014 17:45:27 -0600 6, 26 -- Received: by mail-yk0-f175.google.com with SMTP id 9so726699ykp.34 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 07 Nov 2014 15:45:26 -0800 (PST) 6, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=20120113; 6, 26 -- h=mime-version:date:message-id:subject:from:to:content-type; 6, 26 -- bh=mnNk+7m3XSpGNRBZPDemXlgqRqPTZOMpeqQkOrulCoQ=; 6, 26 -- b=G+rzhAiLc8lT2sIfMoTE8f5sM8B+Li6uJLNdediNiqFgKJMOEbdmf7ODT3WG83Lzyq 6, 26 -- 2qz8qpH1KDa8sA/v2NgRNhC3E6iGCDzcfYqCBbwKfEIqP2UA6byI+2y0U5n8IVo2fzwJ 6, 26 -- wPahSo2yw8eivvJ9ILJkf2VK7VqPV06xRcvbnAd2WGbtDJoNdVbNvO6q2A0iWd80V2iR 6, 26 -- DWSOxrojtrcPwYiRJJMi+cdM4LDGBfHxWh9L2+157MbplnwpOkAO20jthnlaYbltP0vG 6, 26 -- CeCJi5itIu/VSDToUpAF7zZBLkFNqUpZWWDqyD717NC1MBSB9ae0Zih4uY9Mh2r7qVXR 6, 26 -- MDzg== 6, 26 -- MIME-Version: 1.0 6, 26 -- X-Received: by 10.170.196.72 with SMTP id n69mr16222082yke.41.1415403926696; 6, 26 -- Fri, 07 Nov 2014 15:45:26 -0800 (PST) 6, 26 -- Received: by 10.170.180.69 with HTTP; Fri, 7 Nov 2014 15:45:26 -0800 (PST) 6, 26 -- Date: Fri, 7 Nov 2014 18:45:26 -0500 6, 26 -- Message-ID: {CAA8T2PNXGM1Oz+uFQeX=ZeTZfRdFAxfygabj=DCMNoW3wS8M0Q-at-mail.gmail.com} 6, 26 -- Subject: List of aberration corrected electron microscopes in the US? 6, 26 -- From: Christopher Winkler {microwink-at-gmail.com} 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Looking for a vendor for 3 mm diameter TEM grids with a reference pattern for identifying particular areas on the grid both microscopically and macroscopically.
Each individual hole must be between 5 to 10 microns, similar to 2000 mesh grid.
Krassimir Bozhilov.
==============================Original Headers============================== 3, 32 -- From krassimir.bozhilov-at-ucr.edu Fri Nov 7 18:20:46 2014 3, 32 -- Received: from mx2.ucr.edu (mx2.ucr.edu [138.23.62.3]) 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA80KkRt030614 3, 32 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2014 18:20:46 -0600 3, 32 -- X-IronPort-Anti-Spam-Filtered: true 3, 32 -- X-IronPort-Anti-Spam-Result: Au8BAAhhXVSKF/hAnGdsb2JhbABbhB0ijWOuMZgxFgEBAQEBEQEBAQEBCAsJCRQjC4QJOlEBPkInBIhUBaoGnyYBhgwBAQEelEWBHgWQBIMypTmCNIEDAQEB 3, 32 -- X-IronPort-AV: E=Sophos;i="5.07,336,1413270000"; 3, 32 -- d="scan'208";a="274302999" 3, 32 -- Received: from exch-edge-2.ucr.edu ([138.23.248.64]) 3, 32 -- by smtp2.ucr.edu with ESMTP/TLS/AES128-SHA; 07 Nov 2014 16:20:45 -0800 3, 32 -- Received: from EXCH-NODE02.exch.ucr.edu (138.23.248.43) by exch-edge-2.ucr.edu 3, 32 -- (138.23.248.64) with Microsoft SMTP Server (TLS) id 14.3.195.1; Fri, 7 Nov 3, 32 -- 2014 16:20:34 -0800 3, 32 -- Received: from EXCH-MBOX-6.exch.ucr.edu ([169.254.6.209]) by exch-node02 3, 32 -- ([138.23.248.43]) with mapi id 14.03.0195.001; Fri, 7 Nov 2014 16:20:43 -0800 3, 32 -- From: Krassimir Bozhilov {krassimir.bozhilov-at-ucr.edu} 3, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 32 -- Subject: TEM reference grids 3, 32 -- Thread-Topic: TEM reference grids 3, 32 -- Thread-Index: AQHP+unVmH6jrlGmqEqpIwuVqyQlcg== 3, 32 -- Date: Sat, 8 Nov 2014 00:20:43 +0000 3, 32 -- Message-ID: {FD5A6765-E877-4C33-8276-42FF607EAF9B-at-ucr.edu} 3, 32 -- Accept-Language: en-US 3, 32 -- Content-Language: en-US 3, 32 -- X-MS-Has-Attach: 3, 32 -- X-MS-TNEF-Correlator: 3, 32 -- x-originating-ip: [138.23.63.83] 3, 32 -- Content-Type: text/plain; charset="us-ascii" 3, 32 -- Content-ID: {3F633A999A59974A970319D6099891DC-at-exch.ucr.edu} 3, 32 -- MIME-Version: 1.0 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sA80KkRt030614 ==============================End of - Headers==============================
I am not sure what is your particular TEM application, but if you can at all use widely available 3mm TEM grids with SiN windows, then they are fairly easy to mark or pattern on 5um to 10um scale with FIB...
Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
On 11/7/2014 7:22 PM, krassimir.bozhilov-at-ucr.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Looking for a vendor for 3 mm diameter TEM grids with a reference pattern } for identifying particular areas on the grid both microscopically and macroscopically. } } Each individual hole must be between 5 to 10 microns, similar to 2000 mesh grid. } } Krassimir Bozhilov. } } ==============================Original Headers============================== } 3, 32 -- From krassimir.bozhilov-at-ucr.edu Fri Nov 7 18:20:46 2014 } 3, 32 -- Received: from mx2.ucr.edu (mx2.ucr.edu [138.23.62.3]) } 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA80KkRt030614 } 3, 32 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2014 18:20:46 -0600 } 3, 32 -- X-IronPort-Anti-Spam-Filtered: true } 3, 32 -- X-IronPort-Anti-Spam-Result: Au8BAAhhXVSKF/hAnGdsb2JhbABbhB0ijWOuMZgxFgEBAQEBEQEBAQEBCAsJCRQjC4QJOlEBPkInBIhUBaoGnyYBhgwBAQEelEWBHgWQBIMypTmCNIEDAQEB } 3, 32 -- X-IronPort-AV: E=Sophos;i="5.07,336,1413270000"; } 3, 32 -- d="scan'208";a="274302999" } 3, 32 -- Received: from exch-edge-2.ucr.edu ([138.23.248.64]) } 3, 32 -- by smtp2.ucr.edu with ESMTP/TLS/AES128-SHA; 07 Nov 2014 16:20:45 -0800 } 3, 32 -- Received: from EXCH-NODE02.exch.ucr.edu (138.23.248.43) by exch-edge-2.ucr.edu } 3, 32 -- (138.23.248.64) with Microsoft SMTP Server (TLS) id 14.3.195.1; Fri, 7 Nov } 3, 32 -- 2014 16:20:34 -0800 } 3, 32 -- Received: from EXCH-MBOX-6.exch.ucr.edu ([169.254.6.209]) by exch-node02 } 3, 32 -- ([138.23.248.43]) with mapi id 14.03.0195.001; Fri, 7 Nov 2014 16:20:43 -0800 } 3, 32 -- From: Krassimir Bozhilov {krassimir.bozhilov-at-ucr.edu} } 3, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 3, 32 -- Subject: TEM reference grids } 3, 32 -- Thread-Topic: TEM reference grids } 3, 32 -- Thread-Index: AQHP+unVmH6jrlGmqEqpIwuVqyQlcg== } 3, 32 -- Date: Sat, 8 Nov 2014 00:20:43 +0000 } 3, 32 -- Message-ID: {FD5A6765-E877-4C33-8276-42FF607EAF9B-at-ucr.edu} } 3, 32 -- Accept-Language: en-US } 3, 32 -- Content-Language: en-US } 3, 32 -- X-MS-Has-Attach: } 3, 32 -- X-MS-TNEF-Correlator: } 3, 32 -- x-originating-ip: [138.23.63.83] } 3, 32 -- Content-Type: text/plain; charset="us-ascii" } 3, 32 -- Content-ID: {3F633A999A59974A970319D6099891DC-at-exch.ucr.edu} } 3, 32 -- MIME-Version: 1.0 } 3, 32 -- Content-Transfer-Encoding: 8bit } 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sA80KkRt030614 } ==============================End of - Headers============================== }
I am not sure what is your particular TEM application, but if you can at all use widely available 3mm TEM grids with SiN windows, then they are fairly easy to mark or pattern on 5um to 10um scale with FIB...
Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
On 11/7/2014 7:22 PM, krassimir.bozhilov-at-ucr.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Looking for a vendor for 3 mm diameter TEM grids with a reference pattern } for identifying particular areas on the grid both microscopically and macroscopically. } } Each individual hole must be between 5 to 10 microns, similar to 2000 mesh grid. } } Krassimir Bozhilov. } } ==============================Original Headers============================== } 3, 32 -- From krassimir.bozhilov-at-ucr.edu Fri Nov 7 18:20:46 2014 } 3, 32 -- Received: from mx2.ucr.edu (mx2.ucr.edu [138.23.62.3]) } 3, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sA80KkRt030614 } 3, 32 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2014 18:20:46 -0600 } 3, 32 -- X-IronPort-Anti-Spam-Filtered: true } 3, 32 -- X-IronPort-Anti-Spam-Result: Au8BAAhhXVSKF/hAnGdsb2JhbABbhB0ijWOuMZgxFgEBAQEBEQEBAQEBCAsJCRQjC4QJOlEBPkInBIhUBaoGnyYBhgwBAQEelEWBHgWQBIMypTmCNIEDAQEB } 3, 32 -- X-IronPort-AV: E=Sophos;i="5.07,336,1413270000"; } 3, 32 -- d="scan'208";a="274302999" } 3, 32 -- Received: from exch-edge-2.ucr.edu ([138.23.248.64]) } 3, 32 -- by smtp2.ucr.edu with ESMTP/TLS/AES128-SHA; 07 Nov 2014 16:20:45 -0800 } 3, 32 -- Received: from EXCH-NODE02.exch.ucr.edu (138.23.248.43) by exch-edge-2.ucr.edu } 3, 32 -- (138.23.248.64) with Microsoft SMTP Server (TLS) id 14.3.195.1; Fri, 7 Nov } 3, 32 -- 2014 16:20:34 -0800 } 3, 32 -- Received: from EXCH-MBOX-6.exch.ucr.edu ([169.254.6.209]) by exch-node02 } 3, 32 -- ([138.23.248.43]) with mapi id 14.03.0195.001; Fri, 7 Nov 2014 16:20:43 -0800 } 3, 32 -- From: Krassimir Bozhilov {krassimir.bozhilov-at-ucr.edu} } 3, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 3, 32 -- Subject: TEM reference grids } 3, 32 -- Thread-Topic: TEM reference grids } 3, 32 -- Thread-Index: AQHP+unVmH6jrlGmqEqpIwuVqyQlcg== } 3, 32 -- Date: Sat, 8 Nov 2014 00:20:43 +0000 } 3, 32 -- Message-ID: {FD5A6765-E877-4C33-8276-42FF607EAF9B-at-ucr.edu} } 3, 32 -- Accept-Language: en-US } 3, 32 -- Content-Language: en-US } 3, 32 -- X-MS-Has-Attach: } 3, 32 -- X-MS-TNEF-Correlator: } 3, 32 -- x-originating-ip: [138.23.63.83] } 3, 32 -- Content-Type: text/plain; charset="us-ascii" } 3, 32 -- Content-ID: {3F633A999A59974A970319D6099891DC-at-exch.ucr.edu} } 3, 32 -- MIME-Version: 1.0 } 3, 32 -- Content-Transfer-Encoding: 8bit } 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sA80KkRt030614 } ==============================End of - Headers============================== }
The Midwest Microscopy and Microanalysis Society will hold its final meeting of 2014 on Friday, November 21st, at Baxter Healthcare in Deerfield, IL. The topic is Multidimensional Microscopy - Correlative Approaches. We welcome MAS Tour Speaker Dr. Deborah Hall of Rush University Medical Center, and a host of other exciting speakers. A preliminary announcement can be found on the Meetings page of our website, and a final program will be posted soon:
http://www.midwestmicroscopy.org/
Please join us for an informative program and a chance to meet with local colleagues and vendors. We look forward to seeing you there!
Elaine Schumacher M3S Program Coordinator
Elaine F. Schumacher | Senior Research Scientist McCrone Associates, Inc. . 850 Pasquinelli Drive . Westmont, IL 60559 P. 630.887.7100 | F. 630.887.7417 | www.mccrone.com
==============================Original Headers============================== 9, 45 -- From eschumacher-at-mccrone.com Mon Nov 10 07:59:49 2014 9, 45 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 9, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sAADxn9b019264 9, 45 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 07:59:49 -0600 9, 45 -- X-ASG-Debug-ID: 1415627986-07bbd460483a7b80001-4CH8be 9, 45 -- Received: from TMGEX1.tmg.mccrone.com ([192.168.101.73]) by spam.mccrone.com with ESMTP id uxgU5pY0cK49MoFV for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 07:59:46 -0600 (CST) 9, 45 -- X-Barracuda-Envelope-From: eschumacher-at-mccrone.com 9, 45 -- Received: from TMGEX2.tmg.mccrone.com (192.168.101.74) by 9, 45 -- TMGEX1.tmg.mccrone.com (192.168.101.73) with Microsoft SMTP Server (TLS) id 9, 45 -- 15.0.913.22; Mon, 10 Nov 2014 07:59:46 -0600 9, 45 -- Received: from TMGEX2.tmg.mccrone.com ([fe80::d433:2857:50d8:909e]) by 9, 45 -- TMGEX2.tmg.mccrone.com ([fe80::2c91:47d:9b63:8ba3%16]) with mapi id 9, 45 -- 15.00.0913.011; Mon, 10 Nov 2014 07:59:46 -0600 9, 45 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 9, 45 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 45 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society 9, 45 -- Fall Meeting 9, 45 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis 9, 45 -- Society Fall Meeting 9, 45 -- X-ASG-Orig-Subj: Meeting Announcement: Midwest Microscopy and Microanalysis Society 9, 45 -- Fall Meeting 9, 45 -- Thread-Index: Ac/87pVmyMtwBIf5S2+U40k777YtEQ== 9, 45 -- Date: Mon, 10 Nov 2014 13:59:45 +0000 9, 45 -- Message-ID: {227bcded6b804da8bc1c1ef4c49adb2a-at-TMGEX2.tmg.mccrone.com} 9, 45 -- Accept-Language: en-US 9, 45 -- Content-Language: en-US 9, 45 -- X-MS-Has-Attach: 9, 45 -- X-MS-TNEF-Correlator: 9, 45 -- x-vipre-scanned: 003856A8008726003857F5 9, 45 -- x-originating-ip: [192.168.101.84] 9, 45 -- Content-Type: text/plain; charset="iso-8859-1" 9, 45 -- MIME-Version: 1.0 9, 45 -- X-Barracuda-Connect: UNKNOWN[192.168.101.73] 9, 45 -- X-Barracuda-Start-Time: 1415627986 9, 45 -- X-Barracuda-URL: http://spam.mccrone.com:80/cgi-mod/mark.cgi 9, 45 -- X-Virus-Scanned: by bsmtpd at mccrone.com 9, 45 -- X-Barracuda-BRTS-Status: 1 9, 45 -- X-Barracuda-Spam-Score: 0.00 9, 45 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 9, 45 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.11432 9, 45 -- Rule breakdown below 9, 45 -- pts rule name description 9, 45 -- ---- ---------------------- -------------------------------------------------- 9, 45 -- Content-Transfer-Encoding: 8bit 9, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sAADxn9b019264 ==============================End of - Headers==============================
Hi all, I have a antique gold wedding band(circa 1901) with an engraving on the inside that is quite well worn, some parts barely readable under an optical microscope. I mounted it in the SEM, with somewhat better results, but am not able to decipher the entire engraving. Is there any way I could enhance the engraving to make it more legible? I tried wiping it colloidal graphite and then immediately wiping it out lightly to see if the dag would settle in the depressions, but no luck. Thanks.
Gary
Gary M. Easton Scanners Corporation 90 Aileron Court, Ste. 6 Westminster, Maryland USA 21157 410.857.7633 (Office) 443-524-9631(Fax) 717.634.2226 (Mobile)
==============================Original Headers============================== 9, 17 -- From garyeaston-at-scannerscorp.com Mon Nov 10 10:47:12 2014 9, 17 -- Received: from sender1.zohomail.com (sender1.zohomail.com [74.201.84.155]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sAAGlB6n013417 9, 17 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 10:47:12 -0600 9, 17 -- Received: from [192.168.1.101] (69.244.87.213 [69.244.87.213]) by mx.zohomail.com 9, 17 -- with SMTPS id 1415638030410367.1594604270375; Mon, 10 Nov 2014 08:47:10 -0800 (PST) 9, 17 -- From: "Gary Easton" {garyeaston-at-scannerscorp.com} 9, 17 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 17 -- Subject: Gold Ring engraving 9, 17 -- Date: Mon, 10 Nov 2014 16:47:04 +0000 9, 17 -- Message-Id: {emf379f528-4c30-45bb-9ab2-ef701e92e7b8-at-garypc} 9, 17 -- Reply-To: "Gary Easton" {garyeaston-at-scannerscorp.com} 9, 17 -- User-Agent: eM_Client/6.0.21040.0 9, 17 -- Mime-Version: 1.0 9, 17 -- Content-Type: text/plain; format=flowed; charset=utf-8 9, 17 -- Content-Transfer-Encoding: 8bit 9, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sAAGlB6n013417 ==============================End of - Headers==============================
I do not know the answer, but metallography discussion board would seem like a good place to look for engraving-in-metal enhancement techniques and to post a question:
http://www.metallography.com/experts/forum.html
Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
On 11/10/2014 11:48 AM, garyeaston-at-scannerscorp.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } I have a antique gold wedding band(circa 1901) with an engraving on the } inside that is quite well worn, some parts barely readable under an } optical microscope. I mounted it in the SEM, with somewhat better } results, but am not able to decipher the entire engraving. Is there any } way I could enhance the engraving to make it more legible? I tried } wiping it colloidal graphite and then immediately wiping it out lightly } to see if the dag would settle in the depressions, but no luck. Thanks. } } Gary } } } Gary M. Easton } Scanners Corporation } 90 Aileron Court, Ste. 6 } Westminster, Maryland USA 21157 } 410.857.7633 (Office) } 443-524-9631(Fax) } 717.634.2226 (Mobile) } } } } } } } ==============================Original Headers============================== } 9, 17 -- From garyeaston-at-scannerscorp.com Mon Nov 10 10:47:12 2014 } 9, 17 -- Received: from sender1.zohomail.com (sender1.zohomail.com [74.201.84.155]) } 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sAAGlB6n013417 } 9, 17 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 10:47:12 -0600 } 9, 17 -- Received: from [192.168.1.101] (69.244.87.213 [69.244.87.213]) by mx.zohomail.com } 9, 17 -- with SMTPS id 1415638030410367.1594604270375; Mon, 10 Nov 2014 08:47:10 -0800 (PST) } 9, 17 -- From: "Gary Easton" {garyeaston-at-scannerscorp.com} } 9, 17 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 9, 17 -- Subject: Gold Ring engraving } 9, 17 -- Date: Mon, 10 Nov 2014 16:47:04 +0000 } 9, 17 -- Message-Id: {emf379f528-4c30-45bb-9ab2-ef701e92e7b8-at-garypc} } 9, 17 -- Reply-To: "Gary Easton" {garyeaston-at-scannerscorp.com} } 9, 17 -- User-Agent: eM_Client/6.0.21040.0 } 9, 17 -- Mime-Version: 1.0 } 9, 17 -- Content-Type: text/plain; format=flowed; charset=utf-8 } 9, 17 -- Content-Transfer-Encoding: 8bit } 9, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sAAGlB6n013417 } ==============================End of - Headers============================== }
I'd try grazing-incidence light. Have the illumination parallel to the metal surface. This will give the best chance of seeing surface topography. In the SEM, have you tried Y-modulation scanning with BSE in the topography mode?
Phil
} Hi all, } I have a antique gold wedding band(circa 1901) with an engraving on the } inside that is quite well worn, some parts barely readable under an } optical microscope. I mounted it in the SEM, with somewhat better } results, but am not able to decipher the entire engraving. Is there any } way I could enhance the engraving to make it more legible? I tried } wiping it colloidal graphite and then immediately wiping it out lightly } to see if the dag would settle in the depressions, but no luck. Thanks. } } Gary } } } Gary M. Easton } Scanners Corporation } 90 Aileron Court, Ste. 6 } Westminster, Maryland USA 21157 } 410.857.7633 (Office) } 443-524-9631(Fax) } 717.634.2226 (Mobile) -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 35 -- From oshel1pe-at-cmich.edu Mon Nov 10 13:31:00 2014 4, 35 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sAAJUxRf026260 4, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 13:31:00 -0600 4, 35 -- Received: from cas2.central.cmich.local (async2.cmich.edu [141.209.15.141] (may be forged)) 4, 35 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id sAAJUYGa021785 4, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 14:30:59 -0500 4, 35 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 4, 35 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 4, 35 -- (TLS) id 14.3.195.1; Mon, 10 Nov 2014 14:30:57 -0500 4, 35 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 4, 35 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 4, 35 -- (TLS) id 14.3.195.1; Mon, 10 Nov 2014 14:30:56 -0500 4, 35 -- Received: from bio-br024c-mac01.local (141.209.160.122) by 4, 35 -- cas1.central.cmich.local (141.209.15.40) with Microsoft SMTP Server (TLS) id 4, 35 -- 14.3.195.1; Mon, 10 Nov 2014 14:30:56 -0500 4, 35 -- Message-ID: {54611270.8050304-at-cmich.edu} 4, 35 -- Date: Mon, 10 Nov 2014 14:30:56 -0500 4, 35 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 35 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 35 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 35 -- MIME-Version: 1.0 4, 35 -- To: micro {Microscopy-at-microscopy.com} 4, 35 -- Subject: Re: [Microscopy] Gold Ring engraving 4, 35 -- References: {201411101700.sAAH0uIC030825-at-ns.microscopy.com} 4, 35 -- In-Reply-To: {201411101700.sAAH0uIC030825-at-ns.microscopy.com} 4, 35 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 35 -- Content-Transfer-Encoding: 7bit 4, 35 -- X-Originating-IP: [141.209.160.122] 4, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 35 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 4, 35 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 4, 35 -- X-CanItPRO-Stream: default 4, 35 -- X-Canit-Stats-ID: 02NdTuXp4 - 17db44c8fa89 - 20141110 4, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Gary, Have you tried the side shot BSE using the SE detector? Just a thought.
One other thought: The engraving should disturb the crystal structure of the gold, so it might be visible from an overhead BSE without etching the gold, as long as the general surface hasn't been smeared. Might be worth a try. Ken
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: garyeaston-at-scannerscorp.com [mailto:garyeaston-at-scannerscorp.com] Sent: Monday, November 10, 2014 11:50 AM To: kenconverse-at-qualityimages.biz
Hi all, I have a antique gold wedding band(circa 1901) with an engraving on the inside that is quite well worn, some parts barely readable under an optical microscope. I mounted it in the SEM, with somewhat better results, but am not able to decipher the entire engraving. Is there any way I could enhance the engraving to make it more legible? I tried wiping it colloidal graphite and then immediately wiping it out lightly to see if the dag would settle in the depressions, but no luck. Thanks.
Gary
Gary M. Easton Scanners Corporation 90 Aileron Court, Ste. 6 Westminster, Maryland USA 21157 410.857.7633 (Office) 443-524-9631(Fax) 717.634.2226 (Mobile)
==============================Original Headers============================== 9, 17 -- From garyeaston-at-scannerscorp.com Mon Nov 10 10:47:12 2014 9, 17 -- Received: from sender1.zohomail.com (sender1.zohomail.com [74.201.84.155]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sAAGlB6n013417 9, 17 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 10:47:12 -0600 9, 17 -- Received: from [192.168.1.101] (69.244.87.213 [69.244.87.213]) by mx.zohomail.com 9, 17 -- with SMTPS id 1415638030410367.1594604270375; Mon, 10 Nov 2014 08:47:10 -0800 (PST) 9, 17 -- From: "Gary Easton" {garyeaston-at-scannerscorp.com} 9, 17 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 17 -- Subject: Gold Ring engraving 9, 17 -- Date: Mon, 10 Nov 2014 16:47:04 +0000 9, 17 -- Message-Id: {emf379f528-4c30-45bb-9ab2-ef701e92e7b8-at-garypc} 9, 17 -- Reply-To: "Gary Easton" {garyeaston-at-scannerscorp.com} 9, 17 -- User-Agent: eM_Client/6.0.21040.0 9, 17 -- Mime-Version: 1.0 9, 17 -- Content-Type: text/plain; format=flowed; charset=utf-8 9, 17 -- Content-Transfer-Encoding: 8bit 9, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sAAGlB6n013417 ==============================End of - Headers==============================
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==============================Original Headers============================== 21, 28 -- From kenconverse-at-qualityimages.biz Mon Nov 10 13:50:28 2014 21, 28 -- Received: from cdptpa-oedge-vip.email.rr.com (cdptpa-outbound-snat.email.rr.com [107.14.166.231]) 21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sAAJoSZP013740 21, 28 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 13:50:28 -0600 21, 28 -- Received: from [108.183.173.180] ([108.183.173.180:1326] helo=Ken) 21, 28 -- by cdptpa-oedge03 (envelope-from {kenconverse-at-qualityimages.biz} ) 21, 28 -- (ecelerity 3.5.0.35861 r(Momo-dev:tip)) with ESMTP 21, 28 -- id 63/C2-22064-30711645; Mon, 10 Nov 2014 19:50:28 +0000 21, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 28 -- To: {garyeaston-at-scannerscorp.com} , 21, 28 -- "MSA Listserver" {microscopy-at-microscopy.com} 21, 28 -- Subject: RE: [Microscopy] Gold Ring engraving 21, 28 -- Date: Mon, 10 Nov 2014 14:51:06 -0500 21, 28 -- Message-ID: {F59C6A6802294C51AB9AA94691756FC3-at-Ken} 21, 28 -- MIME-Version: 1.0 21, 28 -- Content-Type: text/plain; 21, 28 -- charset="us-ascii" 21, 28 -- X-Priority: 3 (Normal) 21, 28 -- X-MSMail-Priority: Normal 21, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 21, 28 -- In-Reply-To: {201411101650.sAAGoSsC016535-at-ns.microscopy.com} 21, 28 -- Importance: Normal 21, 28 -- Thread-Index: Ac/9BnynMeUivg2nSiek1W+sCZWP2wAGGGDg 21, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 21, 28 -- X-RR-Connecting-IP: 107.14.168.142:25 21, 28 -- X-Cloudmark-Score: 0 21, 28 -- Content-Transfer-Encoding: 8bit 21, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sAAJoSZP013740 ==============================End of - Headers==============================
} We have a bad vibration problem in our building. Our old vibration pad has given up the ghost and is no longer made.
} Would like a recommendation for a anti vibration pad that can be up to four inches thick to place under the microtome to damp the vibrations, which are low frequency building vibrations…Many thanks..tom Reese, NIH
==============================Original Headers============================== 4, 38 -- From treese-at-mbl.edu Mon Nov 10 14:11:56 2014 4, 38 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu [128.128.172.17]) 4, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sAAKBusM001357 4, 38 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 14:11:56 -0600 4, 38 -- Received: from mailfrontier.mbl.edu (127.0.0.1) id hc4e0o0171sk for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 15:11:56 -0500 (envelope-from {treese-at-mbl.edu} ) 4, 38 -- Received: from mail.mbl.edu ([128.128.172.47]) 4, 38 -- by mailfrontier.mbl.edu (SonicWALL 7.4.6.1945) 4, 38 -- with ESMTP id 201411102011550008444; Mon, 10 Nov 2014 15:11:55 -0500 4, 38 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 4, 38 -- by mail.mbl.edu (Postfix) with ESMTP id E68DB1730012; 4, 38 -- Mon, 10 Nov 2014 15:11:55 -0500 (EST) 4, 38 -- Received: from mail.mbl.edu ([127.0.0.1]) 4, 38 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10032) 4, 38 -- with ESMTP id IIc_p8OMhKlY; Mon, 10 Nov 2014 15:11:46 -0500 (EST) 4, 38 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 4, 38 -- by mail.mbl.edu (Postfix) with ESMTP id 11E2B1731276; 4, 38 -- Mon, 10 Nov 2014 15:11:46 -0500 (EST) 4, 38 -- X-Virus-Scanned: amavisd-new at mbl.edu 4, 38 -- Received: from mail.mbl.edu ([127.0.0.1]) 4, 38 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10026) 4, 38 -- with ESMTP id 1WMcpUoTVnUF; Mon, 10 Nov 2014 15:11:45 -0500 (EST) 4, 38 -- Received: from [10.177.196.96] (unknown [156.40.255.21]) 4, 38 -- by mail.mbl.edu (Postfix) with ESMTPSA id 8E06F1730012; 4, 38 -- Mon, 10 Nov 2014 15:11:45 -0500 (EST) 4, 38 -- Content-Type: text/plain; charset=windows-1252 4, 38 -- Mime-Version: 1.0 (Mac OS X Mail 6.6 \(1510\)) 4, 38 -- Subject: vibration table for Reichert 4, 38 -- From: TREESE {treese-at-mbl.edu} 4, 38 -- In-Reply-To: {201411101655.sAAGtWkm022580-at-ns.microscopy.com} 4, 38 -- Date: Mon, 10 Nov 2014 15:11:45 -0500 4, 38 -- Message-Id: {4DBDECAA-F3A9-4F54-9F82-E6BE52A6781D-at-mbl.edu} 4, 38 -- References: {201411101655.sAAGtWkm022580-at-ns.microscopy.com} 4, 38 -- To: microscopy-at-microscopy.com 4, 38 -- X-Mailer: Apple Mail (2.1510) 4, 38 -- X-Mlf-Version: 7.4.6.1945 4, 38 -- X-Mlf-UniqueId: o201411102011550008444 4, 38 -- Content-Transfer-Encoding: 8bit 4, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sAAKBusM001357 ==============================End of - Headers==============================
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Email: Manderson-at-advancedphotonix.com Name: Mark Anderson
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Title-Subject: [Filtered] Tunable Light Source
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==============================Original Headers============================== 18, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Nov 10 17:17:53 2014 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sAANHrOK027705 18, 28 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 17:17:53 -0600 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id 9CAD7782843 18, 28 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 17:17:53 -0600 (CST) 18, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id bqK+qSXm7BP1 for {microscopy-at-microscopy.com} ; 18, 28 -- Mon, 10 Nov 2014 17:17:46 -0600 (CST) 18, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id 9785E782834 18, 28 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 17:17:46 -0600 (CST) 18, 28 -- Message-ID: {5461479A.7010407-at-microscopy.com} 18, 28 -- Date: Mon, 10 Nov 2014 17:17:46 -0600 18, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 18, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 18, 28 -- MIME-Version: 1.0 18, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 28 -- Subject: viaWWW:Tunable LED Light Source 18, 28 -- References: {201411102120.sAALKaDd022456-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201411102120.sAALKaDd022456-at-ns.microscopy.com} 18, 28 -- X-Forwarded-Message-Id: {201411102120.sAALKaDd022456-at-ns.microscopy.com} 18, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Reese, I've had some good success with Newport's "VIBe" series which are easily configurable tables and they aren't as costly as the air based tables. https://www.newport.com/VIBe-Mechanical-Vibration-Isolator-Platforms/987419/ 1033/info.aspx
For a really inexpensive solution - you can try the Meade or Celestron telescope vibration isolators - pack of 3 for about $50. One of my customers used these under their Keyence digital scope and now they can get good images at 2000X - they have a cooling tower with big fan motors right outside on the roof near their lab.
Mike Toalson Western Analytical Solutions, LLC o • 208.899.4425 m • 208.340.3895 w • www.materialanalyzers.com e • mike-at-materialanalyzers.com
-----Original Message----- X-from: treese-at-mbl.edu [mailto:treese-at-mbl.edu] Sent: Monday, November 10, 2014 1:23 PM To: mike-at-materialanalyzers.com
} We have a bad vibration problem in our building. Our old vibration pad has given up the ghost and is no longer made.
} Would like a recommendation for a anti vibration pad that can be up to four inches thick to place under the microtome to damp the vibrations, which are low frequency building vibrations…Many thanks..tom Reese, NIH
==============================Original Headers============================== 4, 38 -- From treese-at-mbl.edu Mon Nov 10 14:11:56 2014 4, 38 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu [128.128.172.17]) 4, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sAAKBusM001357 4, 38 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 14:11:56 -0600 4, 38 -- Received: from mailfrontier.mbl.edu (127.0.0.1) id hc4e0o0171sk for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 15:11:56 -0500 (envelope-from {treese-at-mbl.edu} ) 4, 38 -- Received: from mail.mbl.edu ([128.128.172.47]) 4, 38 -- by mailfrontier.mbl.edu (SonicWALL 7.4.6.1945) 4, 38 -- with ESMTP id 201411102011550008444; Mon, 10 Nov 2014 15:11:55 -0500 4, 38 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 4, 38 -- by mail.mbl.edu (Postfix) with ESMTP id E68DB1730012; 4, 38 -- Mon, 10 Nov 2014 15:11:55 -0500 (EST) 4, 38 -- Received: from mail.mbl.edu ([127.0.0.1]) 4, 38 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10032) 4, 38 -- with ESMTP id IIc_p8OMhKlY; Mon, 10 Nov 2014 15:11:46 -0500 (EST) 4, 38 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 4, 38 -- by mail.mbl.edu (Postfix) with ESMTP id 11E2B1731276; 4, 38 -- Mon, 10 Nov 2014 15:11:46 -0500 (EST) 4, 38 -- X-Virus-Scanned: amavisd-new at mbl.edu 4, 38 -- Received: from mail.mbl.edu ([127.0.0.1]) 4, 38 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10026) 4, 38 -- with ESMTP id 1WMcpUoTVnUF; Mon, 10 Nov 2014 15:11:45 -0500 (EST) 4, 38 -- Received: from [10.177.196.96] (unknown [156.40.255.21]) 4, 38 -- by mail.mbl.edu (Postfix) with ESMTPSA id 8E06F1730012; 4, 38 -- Mon, 10 Nov 2014 15:11:45 -0500 (EST) 4, 38 -- Content-Type: text/plain; charset=windows-1252 4, 38 -- Mime-Version: 1.0 (Mac OS X Mail 6.6 \(1510\)) 4, 38 -- Subject: vibration table for Reichert 4, 38 -- From: TREESE {treese-at-mbl.edu} 4, 38 -- In-Reply-To: {201411101655.sAAGtWkm022580-at-ns.microscopy.com} 4, 38 -- Date: Mon, 10 Nov 2014 15:11:45 -0500 4, 38 -- Message-Id: {4DBDECAA-F3A9-4F54-9F82-E6BE52A6781D-at-mbl.edu} 4, 38 -- References: {201411101655.sAAGtWkm022580-at-ns.microscopy.com} 4, 38 -- To: microscopy-at-microscopy.com 4, 38 -- X-Mailer: Apple Mail (2.1510) 4, 38 -- X-Mlf-Version: 7.4.6.1945 4, 38 -- X-Mlf-UniqueId: o201411102011550008444 4, 38 -- Content-Transfer-Encoding: 8bit 4, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sAAKBusM001357 ==============================End of - Headers==============================
For another low-cost vibration-isolation solution, consider contacting your local monument making vendor and orderring 12" thick granite plate of suitable dimensions. To isolate granite place from the table vibrations use tennis balls from your local sports supply store. Quantity of tennis ball you will need depends on the mass of granite plate and you instrument - try to have them compressed anywhere from 10% to 25% of the tennis ball diameter.
Best of luck!
Valery Ray - also with NISP, UMDCP Nanocenter ============================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-Mail: vray-at-partbeamsystech.com UMD E-Mail: vray-at-umd.edu Web: www.partbeamsystech.com Web: www.freudlabs.com
On 11/10/2014 11:33 PM, mike-at-materialanalyzers.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Reese, } I've had some good success with Newport's "VIBe" series which are easily } configurable tables and they aren't as costly as the air based tables. } https://www.newport.com/VIBe-Mechanical-Vibration-Isolator-Platforms/987419/ } 1033/info.aspx } } For a really inexpensive solution - you can try the Meade or Celestron } telescope vibration isolators - pack of 3 for about $50. One of my } customers used these under their Keyence digital scope and now they can get } good images at 2000X - they have a cooling tower with big fan motors right } outside on the roof near their lab. } } Mike Toalson } Western Analytical Solutions, LLC } o • 208.899.4425 m • 208.340.3895 } w • www.materialanalyzers.com e • mike-at-materialanalyzers.com } } Representing: } HITACHI table-top SEM/EDS, LEICA Microsystems EM Sample Prep } (www.marinereef.com) } APPLIED RIGAKU TECHNOLOGIES XRF Elemental Analyzers } (www.rigakuedxrf.com) } RIGAKU RAMAN TECHNOLOGIES Raman Molecular Spectrometers } (www.rigakuraman.com) } } } -----Original Message----- } X-from: treese-at-mbl.edu [mailto:treese-at-mbl.edu] } Sent: Monday, November 10, 2014 1:23 PM } To: mike-at-materialanalyzers.com } Subject: [Microscopy] vibration table for Reichert } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } We have a bad vibration problem in our building. Our old vibration pad } has given up the ghost and is no longer made. } } } Would like a recommendation for a anti vibration pad that can be up to } four inches thick to place under the microtome to damp the vibrations, which } are low frequency building vibrations…Many thanks..tom Reese, NIH } } } } ==============================Original Headers============================== } 4, 38 -- From treese-at-mbl.edu Mon Nov 10 14:11:56 2014 } 4, 38 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu } [128.128.172.17]) } 4, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } sAAKBusM001357 } 4, 38 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 14:11:56 } -0600 } 4, 38 -- Received: from mailfrontier.mbl.edu (127.0.0.1) id hc4e0o0171sk for } {microscopy-at-microscopy.com} ; Mon, 10 Nov 2014 15:11:56 -0500 (envelope-from } {treese-at-mbl.edu} ) } 4, 38 -- Received: from mail.mbl.edu ([128.128.172.47]) } 4, 38 -- by mailfrontier.mbl.edu (SonicWALL 7.4.6.1945) } 4, 38 -- with ESMTP id 201411102011550008444; 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Email: andel-at-cperi.certh.gr Name: Andreas Delimitis
Organization: CPERI/CERTH
Title-Subject: [Filtered] SEM Wehnelt cup corrosion
Message: Dear listers,
I am contacting you regarding the JEOL 6400 SEM and, particularly, its Wehnelt unit.
On the three last times we exchanged the filament, we noticed that the Wehnelt cup at its outer surface hole (where the edge of the W filament is located inside) appeared to be like corroded or melt. The effect was more pronounced each time we removed the Wehnelt to exchange filament. Furthermore, every new filament was placed into the centre of the cup hole and the gun operates just below saturation conditions.
Any advice why this is happening and about possible ways to prevent it will be highly appreciated.
Thanks in advance, Andreas.
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Nov 11 06:55:26 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sABCtPEL001359 16, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2014 06:55:25 -0600 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id B9878782EDF 16, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2014 06:55:25 -0600 (CST) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id 1awuffsBNgWO for {microscopy-at-microscopy.com} ; 16, 28 -- Tue, 11 Nov 2014 06:55:03 -0600 (CST) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 2E4E7782ECB 16, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2014 06:55:03 -0600 (CST) 16, 28 -- Message-ID: {54620726.8070905-at-microscopy.com} 16, 28 -- Date: Tue, 11 Nov 2014 06:55:02 -0600 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:SEM Wehnelt cup corrosion 16, 28 -- References: {201411110930.sAB9UbYH031157-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201411110930.sAB9UbYH031157-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201411110930.sAB9UbYH031157-at-ns.microscopy.com} 16, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Andreas, it seems you have flash-overs from the high voltage. Check your vacuum in the gun section. Do you have a stable imaging or does the image shift occasionally?
Best regards, Stefan
Am 11.11.2014 14:01, schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: andel-at-cperi.certh.gr () } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both andel-at-cperi.certh.gr as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: andel-at-cperi.certh.gr } Name: Andreas Delimitis } } Organization: CPERI/CERTH } } Title-Subject: [Filtered] SEM Wehnelt cup corrosion } } Message: Dear listers, } } I am contacting you regarding the JEOL 6400 SEM and, particularly, its Wehnelt unit. } } On the three last times we exchanged the filament, we noticed that the Wehnelt cup at its outer } surface hole (where the edge of the W filament is located inside) appeared to be like corroded or } melt. The effect was more pronounced each time we removed the Wehnelt to exchange filament. } Furthermore, every new filament was placed into the centre of the cup hole and the gun operates just } below saturation conditions. } } Any advice why this is happening and about possible ways to prevent it will be highly appreciated. } } Thanks in advance, } Andreas. } } } Login Host: 160.40.21.73 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
At the University of Pennsylvania I used a table constructed by using cinderblocks for the support legs and a huge cement slab that I think is commercially available as flooring in new buildings. This slab was approximately 4 inches thick but maybe 6 feet long. Cement held the whole thing together. A word of caution and that is to make sure that the table does not touch any walls or the small vibrations that flow down the walls will be transferred to your microtome.
Valery's suggestion has the potential of being nicer to look at than the cement that I used and I have heard of using tennis balls previously but have not tried them myself.
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Pat
Patricia Stranen Connelly Research Assistant NHLBI EM Core Facility National Institutes of Health Building 14E Room 111B Bethesda, MD 20892-5570 301-496-3491 connellyps-at-mail.nih.gov
On 11/11/14 2:03 AM, "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} wrote:
} } For another low-cost vibration-isolation solution, consider contacting } your local monument making vendor and orderring 12" thick granite plate } of suitable dimensions. To isolate granite place from the table } vibrations use tennis balls from your local sports supply store. } Quantity of tennis ball you will need depends on the mass of granite } plate and you instrument - try to have them compressed anywhere from 10% } to 25% of the tennis ball diameter. } } Best of luck! } } Valery Ray - also with NISP, UMDCP Nanocenter } ============================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 - leave a message with call-back number } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-Mail: vray-at-partbeamsystech.com } UMD E-Mail: vray-at-umd.edu } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 11/10/2014 11:33 PM, mike-at-materialanalyzers.com wrote: } } } } ------------------------------------------------------------------------- } } --- } } } } Reese, } } I've had some good success with Newport's "VIBe" series which are easily } } configurable tables and they aren't as costly as the air based tables. } } } } https://www.newport.com/VIBe-Mechanical-Vibration-Isolator-Platforms/9874 } } 19/ } } 1033/info.aspx } } } } For a really inexpensive solution - you can try the Meade or Celestron } } telescope vibration isolators - pack of 3 for about $50. One of my } } customers used these under their Keyence digital scope and now they can } } get } } good images at 2000X - they have a cooling tower with big fan motors } } right } } outside on the roof near their lab. } } } } Mike Toalson } } Western Analytical Solutions, LLC } } o € 208.899.4425 m € 208.340.3895 } } w € www.materialanalyzers.com e € mike-at-materialanalyzers.com } } } } Representing: } } HITACHI table-top SEM/EDS, LEICA Microsystems EM Sample Prep } } (www.marinereef.com) } } APPLIED RIGAKU TECHNOLOGIES XRF Elemental Analyzers } } (www.rigakuedxrf.com) } } RIGAKU RAMAN TECHNOLOGIES Raman Molecular Spectrometers } } (www.rigakuraman.com) } } } } } } -----Original Message----- } } X-from: treese-at-mbl.edu [mailto:treese-at-mbl.edu] } } Sent: Monday, November 10, 2014 1:23 PM } } To: mike-at-materialanalyzers.com } } Subject: [Microscopy] vibration table for Reichert } } } } } } } } } We have a bad vibration problem in our building. Our old vibration pad } } has given up the ghost and is no longer made. } } } } } Would like a recommendation for a anti vibration pad that can be up to } } four inches thick to place under the microtome to damp the vibrations, } } which } } are low frequency building vibrationsŠMany thanks..tom Reese, NIH
==============================Original Headers============================== 11, 33 -- From connellyps-at-nhlbi.nih.gov Tue Nov 11 09:02:11 2014 11, 33 -- Received: from nihtlsxway6.hub.nih.gov (nihtlsxway6.hub.nih.gov [128.231.90.89]) 11, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sABF2A0v011008 11, 33 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2014 09:02:10 -0600 11, 33 -- X-IronPort-Anti-Spam-Filtered: true 11, 33 -- X-IronPort-Anti-Spam-Result: AnQDADokYlScKEeH/2dsb2JhbABZA4MPVF3MJIZ4VQKBFxYBAQEBAQN6ghInBiwBCAQJAQEBAw4CCBkODxICGQEBAQQSDCswDAICAgEIDQkBAScHGwQTFAkIAgQKBAUbB4gfDa03oFMBAQEBAQEBAQEBAQEBAQEBAQEBAQEXBJA/AQEcDRYQBwYLhDoFhjqLdwOLcYE0hm+FFIUfhAqDemwBgQ45gQMBAQE 11, 33 -- X-IPAS-Result: AnQDADokYlScKEeH/2dsb2JhbABZA4MPVF3MJIZ4VQKBFxYBAQEBAQN6ghInBiwBCAQJAQEBAw4CCBkODxICGQEBAQQSDCswDAICAgEIDQkBAScHGwQTFAkIAgQKBAUbB4gfDa03oFMBAQEBAQEBAQEBAQEBAQEBAQEBAQEXBJA/AQEcDRYQBwYLhDoFhjqLdwOLcYE0hm+FFIUfhAqDemwBgQ45gQMBAQE 11, 33 -- Received: from unknown (HELO msgb05.nih.gov) ([156.40.71.135]) 11, 33 -- by nihxway6out.hub.nih.gov with ESMTP/TLS/AES128-SHA; 11 Nov 2014 10:01:56 -0500 11, 33 -- Received: from MSGB10.nih.gov ([169.254.10.92]) by msgb05.nih.gov 11, 33 -- ([169.254.5.128]) with mapi id 14.03.0181.006; Tue, 11 Nov 2014 10:01:56 11, 33 -- -0500 11, 33 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 11, 33 -- To: MSA {microscopy-at-microscopy.com} 11, 33 -- CC: "treese-at-mbl.edu" {treese-at-mbl.edu} 11, 33 -- Subject: Re: [Microscopy] vibration table for Reichert 11, 33 -- Thread-Topic: [Microscopy] vibration table for Reichert 11, 33 -- Thread-Index: AQHP/X2l6zo94bO6mEOwVgmsVdZFgJxbhd+A 11, 33 -- Date: Tue, 11 Nov 2014 15:01:55 +0000 11, 33 -- Message-ID: {D0878C27.6591%connellyps-at-mail.nih.gov} 11, 33 -- References: {201411110703.sAB73mS4026951-at-ns.microscopy.com} 11, 33 -- In-Reply-To: {201411110703.sAB73mS4026951-at-ns.microscopy.com} 11, 33 -- Accept-Language: en-US 11, 33 -- Content-Language: en-US 11, 33 -- X-MS-Has-Attach: 11, 33 -- X-MS-TNEF-Correlator: 11, 33 -- user-agent: Microsoft-MacOutlook/14.4.5.141003 11, 33 -- x-originating-ip: [156.40.71.188] 11, 33 -- Content-Type: text/plain; charset="Windows-1252" 11, 33 -- Content-ID: {9DAA13D17A4A4A4699C2B021D8AD33CC-at-mail.nih.gov} 11, 33 -- MIME-Version: 1.0 11, 33 -- Content-Transfer-Encoding: 8bit 11, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id sABF2A0v011008 ==============================End of - Headers==============================
Andreas, If what you're seeing looks like a peeling surface, then I think that you are not cleaning all of the plated tungsten off of the interior of the wehnelt. When it peels, it changes the electrostatic field and causes imaging problems. Even if it doesn't peel, there is often gas trapped beneath it that will eventually release in small pulses, making your beam jump just a little each time. The trick is to inspect the wehnelt very carefully after cleaning (with a light microscope) and reclean if necessary.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, November 11, 2014 7:59 AM To: kenconverse-at-qualityimages.biz
X-from: andel-at-cperi.certh.gr ()
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andel-at-cperi.certh.gr as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: andel-at-cperi.certh.gr Name: Andreas Delimitis
Organization: CPERI/CERTH
Title-Subject: [Filtered] SEM Wehnelt cup corrosion
Message: Dear listers,
I am contacting you regarding the JEOL 6400 SEM and, particularly, its Wehnelt unit.
On the three last times we exchanged the filament, we noticed that the Wehnelt cup at its outer surface hole (where the edge of the W filament is located inside) appeared to be like corroded or melt. The effect was more pronounced each time we removed the Wehnelt to exchange filament. Furthermore, every new filament was placed into the centre of the cup hole and the gun operates just below saturation conditions.
Any advice why this is happening and about possible ways to prevent it will be highly appreciated.
Thanks in advance, Andreas.
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==============================Original Headers============================== 16, 28 -- From microscopylistserver-noreply-at-microscopy.com Tue Nov 11 06:55:26 2014 16, 28 -- Received: from znl.com ([206.69.208.20]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id sABCtPEL001359 16, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2014 06:55:25 -0600 16, 28 -- Received: from localhost (localhost [127.0.0.1]) 16, 28 -- by znl.com (Postfix) with ESMTP id B9878782EDF 16, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2014 06:55:25 -0600 (CST) 16, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 28 -- Received: from znl.com ([127.0.0.1]) 16, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 28 -- with ESMTP id 1awuffsBNgWO for {microscopy-at-microscopy.com} ; 16, 28 -- Tue, 11 Nov 2014 06:55:03 -0600 (CST) 16, 28 -- Received: from Nestor-Mac-Pro--ZNL.local (mac22.zaluzec.com [206.69.208.22]) 16, 28 -- by znl.com (Postfix) with ESMTPA id 2E4E7782ECB 16, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2014 06:55:03 -0600 (CST) 16, 28 -- Message-ID: {54620726.8070905-at-microscopy.com} 16, 28 -- Date: Tue, 11 Nov 2014 06:55:02 -0600 16, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 16, 28 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:24.0) Gecko/20100101 Thunderbird/24.6.0 16, 28 -- MIME-Version: 1.0 16, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 28 -- Subject: viaWWW:SEM Wehnelt cup corrosion 16, 28 -- References: {201411110930.sAB9UbYH031157-at-ns.microscopy.com} 16, 28 -- In-Reply-To: {201411110930.sAB9UbYH031157-at-ns.microscopy.com} 16, 28 -- X-Forwarded-Message-Id: {201411110930.sAB9UbYH031157-at-ns.microscopy.com} 16, 28 -- Content-Ty