Welcome to the 20th year of operation of the Microscopy ListServer a free service to the world wide microscopy community, sponsored jointly by your Friendly Neighborhood SysOp and the Microscopy Society of America.
During 2011, the ListServer delivered 2471 messages to over 3300 subscribers around the world, with minimal hassels (that I know about). For those of you that are statistics junkies this year you generated 275+ Gb of Email traffic and over 8.1 Million Email messages were sent out this year by my tired little server. As usual you don't want to know how much Junk Mail and spam has been filtered out.
The complete Microscopy ListServer Archives for 2011-1993 (~ are on-line at
http://www.microscopy.com.
A couple of IMPORTANT reminders:
If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE your Email address from the listserver. The out-of-office / on-vacation autoreply messages are a real nuisance to posters.
Do not reply to message with the return address of:
MicroscopyListserver-noreply-at-microscopy.com
these are messages forwarded usually from the WWW posting form. They do not go back to the poster but rather into a black hole, which I rarely check. If you see a message that has this "No-Reply" return address please post your reply/comment/answer to:
Microscopy-at-microscopy.com
or if you wish to reply privately, look at the username in the body of the message the originators Email address is usually listed therein.
As always if you have questions about suitability of postings or are having problems, feel free to contact me at (zaluzec-at-microscopy.com)
Cheers,
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 15, 24 -- From microscopylistserver-noreply-at-microscopy.com Sun Jan 1 08:03:02 2012 15, 24 -- Received: from znl.com ([206.69.208.20]) 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q01E323l018091 15, 24 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 08:03:02 -0600 15, 24 -- Received: from localhost (localhost [127.0.0.1]) 15, 24 -- by znl.com (Postfix) with ESMTP id 2DB8946A4D8 15, 24 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 08:03:02 -0600 (CST) 15, 24 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 24 -- Received: from znl.com ([127.0.0.1]) 15, 24 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 24 -- with ESMTP id j68+gLUBo0mF for {microscopy-at-microscopy.com} ; 15, 24 -- Sun, 1 Jan 2012 08:03:00 -0600 (CST) 15, 24 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 24 -- by znl.com (Postfix) with ESMTPA id 92BC946A4C8 15, 24 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 08:03:00 -0600 (CST) 15, 24 -- Message-ID: {4F006793.8070004-at-microscopy.com} 15, 24 -- Date: Sun, 01 Jan 2012 08:02:59 -0600 15, 24 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 24 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 15, 24 -- MIME-Version: 1.0 15, 24 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 24 -- Subject: Administrivia: Happy New Year - 2012 15, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both isha.mutreja-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: How to process SEM images by adobe photoshop to add coloured effects to those images. Thank you in anticipation. Looking forward for some help and guidance.
here is the permalink to MIAWiki page on the topic: http://confocal-manawatu.pbworks.com/w/page/44308564/Color%20SEM%20Images
Hope that will be helpful.
Cheers, Dmitry MIAWiki for Mass Collaboration
On Mon, January 2, 2012 5:57 am, microscopylistserver-noreply-at-microscopy.com wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both } isha.mutreja-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: isha.mutreja-at-gmail.com } Name: isha mutreja } } Organization: Ulster University } } Title-Subject: [Filtered] Biomedical Sciences } } Message: How to process SEM images by adobe photoshop to add coloured } effects to those images. Thank } you in anticipation. Looking forward for some help and guidance. } } Login Host: 2.220.237.78 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Sun Jan 1 } 10:51:40 2012 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } q01Gpe5S004142 } 8, 25 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 10:51:40 -0600 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id 5282B46A6EE } 8, 25 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 10:51:40 -0600 } (CST) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, } port 10024) } 8, 25 -- with ESMTP id 2hp0K5OGNV-K for {microscopy-at-microscopy.com} ; } 8, 25 -- Sun, 1 Jan 2012 10:51:39 -0600 (CST) } 8, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com } [206.69.208.22]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id 336FC46A6E3 } 8, 25 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 10:51:39 -0600 } (CST) } 8, 25 -- Message-ID: {4F008F1A.1090502-at-microscopy.com} } 8, 25 -- Date: Sun, 01 Jan 2012 10:51:38 -0600 } 8, 25 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: isha.mutreja-at-gmail.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: viaWWW:process SEM images by adobe photoshop } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
-- Dr. Dmitry Sokolov Institute of Fundamental Sciences Massey University, Palmerston North New Zealand
==============================Original Headers============================== 8, 27 -- From d.sokolov-at-massey.ac.nz Sun Jan 1 21:10:42 2012 8, 27 -- Received: from mu-mail2.massey.ac.nz (mu-mail2.massey.ac.nz [130.123.129.7]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q023Ae2j027111 8, 27 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 21:10:41 -0600 8, 27 -- Received: from TUR-MM2.massey.ac.nz (tur-mm2.massey.ac.nz [130.123.128.139]) 8, 27 -- by mu-mail2.massey.ac.nz (Postfix) with ESMTP id 1C09C869F8; 8, 27 -- Mon, 2 Jan 2012 16:10:38 +1300 (NZDT) 8, 27 -- Received: from webmail.massey.ac.nz (Not Verified[10.100.189.3]) by TUR-MM2.massey.ac.nz with MailMarshal 8, 27 -- id {B4f01202d0000} ; Mon, 02 Jan 2012 16:10:37 +1300 8, 27 -- Received: from 125.237.119.137 8, 27 -- (SquirrelMail authenticated user dsokolov) 8, 27 -- by webmail.massey.ac.nz with HTTP; 8, 27 -- Mon, 2 Jan 2012 16:10:37 +1300 (NZDT) 8, 27 -- Message-ID: {59701.125.237.119.137.1325473837.squirrel-at-webmail.massey.ac.nz} 8, 27 -- In-Reply-To: {201201011657.q01Gv1NQ012877-at-ns.microscopy.com} 8, 27 -- References: {201201011657.q01Gv1NQ012877-at-ns.microscopy.com} 8, 27 -- Date: Mon, 2 Jan 2012 16:10:37 +1300 (NZDT) 8, 27 -- Subject: Re: [Microscopy] viaWWW:process SEM images by adobe photoshop 8, 27 -- From: "Dmitry Sokolov" {d.sokolov-at-massey.ac.nz} 8, 27 -- To: Microscopy-at-microscopy.com 8, 27 -- Cc: isha.mutreja-at-gmail.com 8, 27 -- User-Agent: SquirrelMail/1.4.8-5.el5_4.10 8, 27 -- MIME-Version: 1.0 8, 27 -- Content-Type: text/plain;charset=iso-8859-1 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-Priority: 3 (Normal) 8, 27 -- Importance: Normal ==============================End of - Headers==============================
A very Happy New Year to you, too! I would like to thank you for the Fantastic job you do with the listserver. It has been a very positive influence in many lives, and careers.
Best Regards, Darrell Miles
microscopylistserver-noreply-at-microscopy.com wrote on 01/01/2012 09:03:45 AM:
--| [image removed] --| --| [Microscopy] Administrivia: Happy New Year - 2012 --| --| microscopylistserver-noreply --| --| to: --| --| Darrell Miles --| --| 01/01/2012 09:04 AM --| --| Please respond to microscopylistserver-noreply --| --| --| --| --| ---------------------------------------------------------------------------- --| The Microscopy ListServer -- CoSponsor: The Microscopy Society of America --| To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver --| On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html --| ---------------------------------------------------------------------------- --| --| Happy New Year Colleagues; --| --| Welcome to the 20th year of operation of the Microscopy ListServer --| a free service to the world wide microscopy community, sponsored --| jointly by your Friendly Neighborhood SysOp and the Microscopy Society --| of America. --| --| During 2011, the ListServer delivered 2471 messages to over 3300 --| subscribers --| around the world, with minimal hassels (that I know about). For --| those of you that are --| statistics junkies this year you generated 275+ Gb of Email traffic and over --| 8.1 Million Email messages were sent out this year by my tired little server. --| As usual you don't want to know how much Junk Mail and spam has been --| filtered out. --| --| --| The complete Microscopy ListServer Archives for 2011-1993 (~ are on-line at --| --| http://www.microscopy.com. --| --| A couple of IMPORTANT reminders: --| --| If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE --| your Email address from the listserver. The out-of-office / on-vacation --| autoreply messages are a real nuisance to posters. --| --| Do not reply to message with the return address of: --| --| MicroscopyListserver-noreply-at-microscopy.com --| --| these are messages forwarded usually from the WWW posting form. They do not --| go back to the poster but rather into a black hole, which I rarely check. --| If you see a message that has this "No-Reply" return address please post your --| reply/comment/answer to: --| --| Microscopy-at-microscopy.com --| --| or if you wish to reply privately, look at the username in the body of --| the message the originators Email address is usually listed therein. --| --| As always if you have questions about suitability of postings or --| are having problems, feel free to contact me at (zaluzec-at-microscopy.com) --| --| Cheers, --| --| Nestor --| Your Friendly Neighborhood SysOp --| --| ==============================Original Headers============================== --| 15, 24 -- From microscopylistserver-noreply-at-microscopy.com Sun Jan --| 1 08:03:02 2012 --| 15, 24 -- Received: from znl.com ([206.69.208.20]) --| 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with --| ESMTP id q01E323l018091 --| 15, 24 -- for |--microscopy-at-microscopy.com--|; Sun, 1 Jan 2012 08:03:02 -0600 --| 15, 24 -- Received: from localhost (localhost [127.0.0.1]) --| 15, 24 -- by znl.com (Postfix) with ESMTP id 2DB8946A4D8 --| 15, 24 -- for |--microscopy-at-microscopy.com--|; Sun, 1 Jan 2012 08: --| 03:02 -0600 (CST) --| 15, 24 -- X-Virus-Scanned: amavisd-new at localhost.localdomain --| 15, 24 -- Received: from znl.com ([127.0.0.1]) --| 15, 24 -- by localhost (server.microscopy.com [127.0.0.1]) --| (amavisd-new, port 10024) --| 15, 24 -- with ESMTP id j68+gLUBo0mF for |--microscopy-at-microscopy.com--|; --| 15, 24 -- Sun, 1 Jan 2012 08:03:00 -0600 (CST) --| 15, 24 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) --| 15, 24 -- by znl.com (Postfix) with ESMTPA id 92BC946A4C8 --| 15, 24 -- for |--microscopy-at-microscopy.com--|; Sun, 1 Jan 2012 08: --| 03:00 -0600 (CST) --| 15, 24 -- Message-ID: |--4F006793.8070004-at-microscopy.com--| --| 15, 24 -- Date: Sun, 01 Jan 2012 08:02:59 -0600 --| 15, 24 -- From: MicroscopyListserver-NoReply |--microscopylistserver- --| noreply-at-microscopy.com--| --| 15, 24 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10. --| 6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 --| 15, 24 -- MIME-Version: 1.0 --| 15, 24 -- To: MicroscopyListServer-Forward |--microscopy-at-microscopy.com--| --| 15, 24 -- Subject: Administrivia: Happy New Year - 2012 --| 15, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed --| 15, 24 -- Content-Transfer-Encoding: 7bit --| ==============================End of - Headers============================== --|
|--br--||--font size=2 face="sans-serif"--|Dear Nestor,|--/font--| |--br--| |--br--||--font size=2 face="sans-serif"--|A very Happy New Year to you, too! I would like to thank you for the Fantastic job you do with the listserver. It has been a very positive influence in many lives, and careers.|--/font--| |--br--| |--br--||--font size=2 face="sans-serif"--|Best Regards,|--/font--| |--br--||--font size=2 face="sans-serif"--|Darrell Miles|--/font--| |--br--| |--br--| |--br--| |--br--||--tt--||--font size=2--|microscopylistserver-noreply-at-microscopy.com wrote on 01/01/2012 09:03:45 AM:|--br--| |--br--| > [image removed] |--/font--||--/tt--| |--br--||--tt--||--font size=2--|> |--br--| > [Microscopy] Administrivia: Happy New Year - 2012|--/font--||--/tt--| |--br--||--tt--||--font size=2--|> |--br--| > microscopylistserver-noreply |--/font--||--/tt--| |--br--||--tt--||--font size=2--|> |--br--| > to:|--/font--||--/tt--| |--br--||--tt--||--font size=2--|> |--br--| > Darrell Miles|--/font--||--/tt--| |--br--||--tt--||--font size=2--|> |--br--| > 01/01/2012 09:04 AM|--/font--||--/tt--| |--br--||--tt--||--font size=2--|> |--br--| > Please respond to microscopylistserver-noreply|--/font--||--/tt--| |--br--||--tt--||--font size=2--|> |--br--| > |--br--| > |--br--| > |--br--| > ----------------------------------------------------------------------------|--br--| > The Microscopy ListServer -- CoSponsor: The Microscopy Society of America|--br--| > To Subscribe/Unsubscribe -- |--/font--||--/tt--||--a href=http://www.microscopy.com/MicroscopyListserver--||--tt--||--font size=2--|http://www.microscopy.com/MicroscopyListserver|--/font--||--/tt--||--/a--||--tt--||--font size=2--||--br--| > On-Line Help |--/font--||--/tt--||--a href=http://www.microscopy.com/MicroscopyListserver/FAQ.html--||--tt--||--font size=2--|http://www.microscopy.com/MicroscopyListserver/FAQ.html|--/font--||--/tt--||--/a--||--tt--||--font size=2--||--br--| > ----------------------------------------------------------------------------|--br--| > |--br--| > Happy New Year Colleagues;|--br--| > |--br--| > Welcome to the 20th year of operation of the Microscopy ListServer|--br--| > a free service to the world wide microscopy community, sponsored|--br--| > jointly by your Friendly Neighborhood SysOp and the Microscopy Society|--br--| > of America.|--br--| > |--br--| > During 2011, the ListServer delivered 2471 messages to over 3300|--br--| > subscribers|--br--| > around the world, with minimal hassels (that I know about). For |--br--| > those of you that are|--br--| > statistics junkies this year you generated 275+ Gb of Email traffic and over|--br--| > 8.1 Million Email messages were sent out this year by my tired little server.|--br--| > As usual you don't want to know how much Junk Mail and spam has been|--br--| > filtered out.|--br--| > |--br--| > |--br--| > The complete Microscopy ListServer Archives for 2011-1993 (~ are on-line at|--br--| > |--br--| > |--/font--||--/tt--||--a href=http://www.microscopy.com/--||--tt--||--font size=2--|http://www.microscopy.com|--/font--||--/tt--||--/a--||--tt--||--font size=2--|.|--br--| > |--br--| > A couple of IMPORTANT reminders:|--br--| > |--br--| > If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE|--br--| > your Email address from the listserver. The out-of-office / on-vacation|--br--| > autoreply messages are a real nuisance to posters.|--br--| > |--br--| > Do not reply to message with the return address of:|--br--| > |--br--| > MicroscopyListserver-noreply-at-microscopy.com|--br--| > |--br--| > these are messages forwarded usually from the WWW posting form. They do not|--br--| > go back to the poster but rather into a black hole, which I rarely check.|--br--| > If you see a message that has this "No-Reply" return address please post your|--br--| > reply/comment/answer to:|--br--| > |--br--| > Microscopy-at-microscopy.com|--br--| > |--br--| > or if you wish to reply privately, look at the username in the body of|--br--| > the message the originators Email address is usually listed therein.|--br--| > |--br--| > As always if you have questions about suitability of postings or|--br--| > are having problems, feel free to contact me at (zaluzec-at-microscopy.com)|--br--| > |--br--| > Cheers,|--br--| > |--br--| > Nestor|--br--| > Your Friendly Neighborhood SysOp|--br--| > |--br--| > ==============================Original Headers==============================|--br--| > 15, 24 -- From microscopylistserver-noreply-at-microscopy.com Sun Jan |--br--| > 1 08:03:02 2012|--br--| > 15, 24 -- Received: from znl.com ([206.69.208.20])|--br--| > 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with |--br--| > ESMTP id q01E323l018091|--br--| > 15, 24 -- for <microscopy-at-microscopy.com>; Sun, 1 Jan 2012 08:03:02 -0600|--br--| > 15, 24 -- Received: from localhost (localhost [127.0.0.1])|--br--| > 15, 24 -- by znl.com (Postfix) with ESMTP id 2DB8946A4D8|--br--| > 15, 24 -- for <microscopy-at-microscopy.com>; Sun, 1 Jan 2012 08:|--br--| > 03:02 -0600 (CST)|--br--| > 15, 24 -- X-Virus-Scanned: amavisd-new at localhost.localdomain|--br--| > 15, 24 -- Received: from znl.com ([127.0.0.1])|--br--| > 15, 24 -- by localhost (server.microscopy.com [127.0.0.1]) |--br--| > (amavisd-new, port 10024)|--br--| > 15, 24 -- with ESMTP id j68+gLUBo0mF for <microscopy-at-microscopy.com>;|--br--| > 15, 24 -- Sun, 1 Jan 2012 08:03:00 -0600 (CST)|--br--| > 15, 24 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22])|--br--| > 15, 24 -- by znl.com (Postfix) with ESMTPA id 92BC946A4C8|--br--| > 15, 24 -- for <microscopy-at-microscopy.com>; Sun, 1 Jan 2012 08:|--br--| > 03:00 -0600 (CST)|--br--| > 15, 24 -- Message-ID: <4F006793.8070004-at-microscopy.com>|--br--| > 15, 24 -- Date: Sun, 01 Jan 2012 08:02:59 -0600|--br--| > 15, 24 -- From: MicroscopyListserver-NoReply <microscopylistserver-|--br--| > noreply-at-microscopy.com>|--br--| > 15, 24 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.|--br--| > 6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4|--br--| > 15, 24 -- MIME-Version: 1.0|--br--| > 15, 24 -- To: MicroscopyListServer-Forward <microscopy-at-microscopy.com>|--br--| > 15, 24 -- Subject: Administrivia: Happy New Year - 2012|--br--| > 15, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed|--br--| > 15, 24 -- Content-Transfer-Encoding: 7bit|--br--| > ==============================End of - Headers==============================|--br--| > |--br--| |--/font--||--/tt--| --=_alternative 005932248525797A_=--
==============================Original Headers============================== 12, 36 -- From milesd-at-us.ibm.com Tue Jan 3 10:16:24 2012 12, 36 -- Received: from e1.ny.us.ibm.com (e1.ny.us.ibm.com [32.97.182.141]) 12, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q03GGLTa030435 12, 36 -- for |--microscopy-at-microscopy.com--|; Tue, 3 Jan 2012 10:16:23 -0600 12, 36 -- Received: from /spool/local 12, 36 -- by e1.ny.us.ibm.com with IBM ESMTP SMTP Gateway: Authorized Use Only! Violators will be prosecuted 12, 36 -- for |--microscopy-at-microscopy.com--| from |--milesd-at-us.ibm.com--|; 12, 36 -- Tue, 3 Jan 2012 11:16:17 -0500 12, 36 -- Received: from d01relay01.pok.ibm.com (9.56.227.233) 12, 36 -- by e1.ny.us.ibm.com (192.168.1.101) with IBM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ban-at-mol.biol.ethz.ch as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ban-at-mol.biol.ethz.ch Name: Nenad Ban
Organization: ETH Zurich
Title-Subject: [Filtered] Assistant professor position at ETH Zurich in electron microscopy
Message: Dear Colleagues, I would like to draw your attention to an assistant professor position in Electron Microscopy / Structural Biology at the ETH Zurich in Switzerland. The text below describes the position that has been advertised in Nature and Science. Details of the position are negotiable depending on the qualifications of the candidate. We encourage excellent candidates to apply, for more information you may contact Prof. Nenad Ban, address provided below.
With best wishes, Nenad Ban
==============================Original Headers============================== 6, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 4 07:28:26 2012 6, 25 -- Received: from znl.com ([206.69.208.20]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q04DSQRG032153 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jan 2012 07:28:26 -0600 6, 25 -- Received: from localhost (localhost [127.0.0.1]) 6, 25 -- by znl.com (Postfix) with ESMTP id 504D046C82B 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jan 2012 07:28:26 -0600 (CST) 6, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 6, 25 -- Received: from znl.com ([127.0.0.1]) 6, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 6, 25 -- with ESMTP id CNczEL+wT9za for {microscopy-at-microscopy.com} ; 6, 25 -- Wed, 4 Jan 2012 07:28:23 -0600 (CST) 6, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 6, 25 -- by znl.com (Postfix) with ESMTPA id BCEDB46C81B 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jan 2012 07:28:23 -0600 (CST) 6, 25 -- Message-ID: {4F0453F6.1030103-at-microscopy.com} 6, 25 -- Date: Wed, 04 Jan 2012 07:28:22 -0600 6, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 6, 25 -- Reply-To: ban-at-mol.biol.ethz.ch 6, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 25 -- MIME-Version: 1.0 6, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 6, 25 -- Subject: viaWWW:Assistant professor position at ETH Zurich in electron microscopy 6, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j.knowles-at-ucl.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: j.knowles-at-ucl.ac.uk Name: Jonathan Knowles
Organization: UCL
Title-Subject: [Filtered] Intergating Prior Stage with BioRad 2100 CLSM
Message: Hi
We have a Prior Stsge and BioRad 2100. I was looking to configure the stage within the LAsersharp software. The manual points to a cfg file, but I am unsure what to change/configure. Can anyone provide some guidance?
I have approximatley 200 ml of 80 mM Ammonium Molybdate solution left by a user in our Lab. The solution was used as a stain for biological specimens. Can anyone familiar with this stain point me to information concerning proper method of disposal.
The MSDS sheet on Ammonium Molybdate indicates care should be taken.
Thanks...
Nestor Your Friendly Neighborhood SysOp.
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 Argonne, Illinois 60439 USA
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America E.P. Wigner Fellow - Oak Ridge National Laboratory Senior Fellow of the Computational Institute - University of Chicago Past President Microscopy Society of America
===========================================
The box said ... "This program requires Win 95/98/NT/2K/XP/Vista or better..." So I bought a Mac !
===========================================
==============================Original Headers============================== 16, 25 -- From zaluzec-at-aaem.amc.anl.gov Wed Jan 4 19:58:54 2012 16, 25 -- Received: from znl.com ([206.69.208.20]) 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q051wsx7007898 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jan 2012 19:58:54 -0600 16, 25 -- Received: from localhost (localhost [127.0.0.1]) 16, 25 -- by znl.com (Postfix) with ESMTP id 84F1F46CF9E 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jan 2012 19:58:54 -0600 (CST) 16, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 16, 25 -- Received: from znl.com ([127.0.0.1]) 16, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 16, 25 -- with ESMTP id YDbUREH8VSii for {microscopy-at-microscopy.com} ; 16, 25 -- Wed, 4 Jan 2012 19:58:53 -0600 (CST) 16, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 16, 25 -- by znl.com (Postfix) with ESMTPA id B1FDD46CF93 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jan 2012 19:58:53 -0600 (CST) 16, 25 -- Message-ID: {4F0503DD.2090907-at-aaem.amc.anl.gov} 16, 25 -- Date: Wed, 04 Jan 2012 19:58:53 -0600 16, 25 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 16, 25 -- Organization: ANL 16, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 16, 25 -- MIME-Version: 1.0 16, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 25 -- Subject: Diposal Advice Needed for Ammonium Molybdate Solution 16, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A postdoctoral position is available at the University of Wisconsin-Madison to work on electron tomography and cryo-electron microscopy of membrane deformation mechanisms mediated by ESCRT proteins. The postdoctoral researcher will work as part of a multidisciplinary team integrated by cell biologists, biophysicists, and virologists to understand structural aspects of ESCRT-mediated membrane budding in multiple organisms/systems, including plants, worms, and mammalian cultured cells.
The project entails imaging of plastic and vitreous sections of plant and animal tissues by electron tomography.
Applicants must have a Ph.D. and proven experience in the field of electron microscopy, electron tomography, and cryo-electron microscopy. Experience with vitreous sectioning and single particle analysis is desirable. Excellent written and oral skills and the ability to work collaboratively with others are required.
For more information of ongoing research in the labs involved in this project, please visit the following websites: http://www.botany.wisc.edu/otegui/welcome.html http://www.bmolchem.wisc.edu/faculty/audhya.html http://www.mcardle.wisc.edu/faculty/bio/ahlquist_p.html
Interested candidates should submit a letter stating experience, and also include a CV and the names and contact information (phone and e-mail) of individuals who can provide a professional letter of references in a single pdf file to Dr Marisa Otegui (otegui-at-wisc.edu).
Marisa Otegui Associate Professor B119 Birge Hall 430 Lincoln Drive Department of Botany Madison, WI 53706 Lab webpage http://www.botany.wisc.edu/otegui/welcome.html
Phone: (608)265-5703 Fax: (608)262-7509
==============================Original Headers============================== 7, 36 -- From marisa_ote-at-yahoo.com Thu Jan 5 07:53:54 2012 7, 36 -- Received: from nm27-vm3.bullet.mail.ne1.yahoo.com (nm27-vm3.bullet.mail.ne1.yahoo.com [98.138.91.157]) 7, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q05DrrJT015674 7, 36 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2012 07:53:54 -0600 7, 36 -- Received: from [98.138.90.57] by nm27.bullet.mail.ne1.yahoo.com with NNFMP; 05 Jan 2012 13:53:52 -0000 7, 36 -- Received: from [98.138.89.198] by tm10.bullet.mail.ne1.yahoo.com with NNFMP; 05 Jan 2012 13:53:52 -0000 7, 36 -- Received: from [127.0.0.1] by omp1056.mail.ne1.yahoo.com with NNFMP; 05 Jan 2012 13:53:52 -0000 7, 36 -- X-Yahoo-Newman-Property: ymail-3 7, 36 -- X-Yahoo-Newman-Id: 503127.52763.bm-at-omp1056.mail.ne1.yahoo.com 7, 36 -- Received: (qmail 95818 invoked by uid 60001); 5 Jan 2012 13:53:51 -0000 7, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1325771631; bh=ixsLQnDWgpT2IbS1+1x097wu/uOmd21j9BKFHVSn4B0=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=NynKQSqkJBYUnQDlYpk+amsRWM4Afmhyf71EwMlwfYYry07ZADbhJbMOo42lnJGFVoRJ1V8X6FD1REi55rxQwPUkMXBKBZBMy3OfVB4La4gkeZdNq526EJ08g6oUe2WE1xiMK6W3JzD07RKmM7bXCbAR9M7CQ2r+YxuyvNFt/II= 7, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 36 -- s=s1024; d=yahoo.com; 7, 36 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 7, 36 -- b=OWqUayCIKN4Fg7FBhNT3Q4QnOwnqvrTHWOzyOcXvnPSoOI6jQf0HbkJV5Ci+PVT7b1j8LcyS//VUpjNL3fbKms6NBltoJaDSw2JTX3D/NSiT0EVUH8kFI1Wgb/ej7FLHu42o1nUS301/S4Jl3JWW/lMDkBT8Xbaj+p0Qniplx8c=; 7, 36 -- X-YMail-OSG: FaxzmWwVM1mLD4HV8c5ozIVcvGQl0mQnquw1c_xqaF389Qv 7, 36 -- xsieKd_j8T0AHDT6q.8MrGjLd7bAl4SBmqEjgCtXlfQyK7pfNETYeGv7377i 7, 36 -- D.owrdBODfNcuPp_518RXc.3qf_ixmr0S2X2gFqhaFygJ9MSbV3d.Jz_59tl 7, 36 -- 8gs2iJwHrXCJl0CCo0p8Ba87aaG94feQ5puo24Itykc1mvJWWoDKOE9w0R3o 7, 36 -- WCc4rzIcD3lQqhvyTPXVXW0yh2GjFHCy.HbLx58VLwDyfkj8Uxs9i5qbo6_X 7, 36 -- PzWLdaRstgdc42prJDyqt8UXvMS9yTATPfbLxKcdccIwx7MYmHwJ50ROaQTu 7, 36 -- 5D0I9WdE.R5.OHlhWDfin0GF1uh.m5MsXhHHft1DciUb7UJl5RAu3yxFfCfX 7, 36 -- rNgVG4LXwnSHbDToAJHKPeZ840gSRRW_3ZL_KZSEfl2QjvhmSudajkg0NENz 7, 36 -- oQMFk 7, 36 -- Received: from [146.151.121.99] by web111413.mail.gq1.yahoo.com via HTTP; Thu, 05 Jan 2012 05:53:51 PST 7, 36 -- X-Mailer: YahooMailWebService/0.8.115.331698 7, 36 -- Message-ID: {1325771631.75496.YahooMailNeo-at-web111413.mail.gq1.yahoo.com} 7, 36 -- Date: Thu, 5 Jan 2012 05:53:51 -0800 (PST) 7, 36 -- From: Marisa Otegui {marisa_ote-at-yahoo.com} 7, 36 -- Reply-To: Marisa Otegui {marisa_ote-at-yahoo.com} 7, 36 -- Subject: postdoctoral position available on cryo-EM and electron tomography 7, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 36 -- MIME-Version: 1.0 7, 36 -- Content-Type: text/plain; charset=iso-8859-1 7, 36 -- Content-Transfer-Encoding: 8bit 7, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q05DrrJT015674 ==============================End of - Headers==============================
As well as Dmitry's www5.pbrc.hawaii.edu/microangela etc links, below is some info copy and pasted from a very similar discussion on colouring B&W TEM images back in January 2010 on the microscopy list-server [to find all these posts, the threads were under the title: 'Image Colorizing']:
I tried out colorizing an SEM image with Photoshop CS4 and the results seemed fine:
Original SEM image of pollen http://en.wikipedia.org/wiki/File:Misc_pollen.jpg
I Colorized the image using the method in my last post, see below [you could use Photoshop CS4 or Elements 8] http://www.well.ox.ac.uk/cytogenetics/pollen/pollen_colorized.jpg It took well under an hour to select and colorize the whole image [and I found it quite therapeutic].
I also applied a Pseudocolor look-up table [LUT] applied using Photoshop CS4 [and Elements 8 has similar tools] http://www.well.ox.ac.uk/cytogenetics/pollen/pollen_pseudo.jpg For details of creating pseudocolor [false colour] with LUTs [e.g. Image, Mode, Color table] with Photoshop CS4 see the likes of http://www.imagingandanalysis.com/pseudo.pdf Adobe's help, and search the internet.
---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------------------ Details of the colouring technique in Photoshop CS4:
Hi Isha,
It is quite easy, if time consuming, to do this Photoshop CS4 or CS5 [CS5 Extended's only £130 with Educational discounts - on a really tight budget the likes Serif PhotoPlus X4 or Adobe Elements 10 will suffice].
There's always different ways to approach something like this in Photoshop CS5 [or earlier versions], but I'd try:
Load the SEM Photo and manually select the region you wish to colourize, using say the 'quick selection tool' - and you can add/reject [shift/alt] bits of selected regions with a lasso tool. Then ensure the image is converted to RGB colour [image, mode]. I'd then go to 'image, adjustments, colour balance' and lightly adjust the colour balance of the selected region and then "you can be brown, you can be blue, you can be violet sky", e.g. move the colour balance slider from cyan to red and the selected object with become progressively more red, with the underlying structural details intact. Then work through the entire image. Select all similar objects [to be the same colour] within the image as one multiple region for colourizing [hold shift as you select]. Make a mistake and use Edit, Undo. You can say slightly adjust contrast and brightness, or curves, or shadow/highlights within those selected regions as well.
You don't really need layers, you could work only on the main image [background] bit by bit - save regularly under new file names as Photoshops Undo [step backwards] is limited if, after a lot of work, you don't like the way the image is turning out.
You could do all this with Photoshop Elements 8 as well [£37 with discounts]. Do all the above Photoshop stuff using the similar Elements selection tools, then [instead of 'colour balance'] go to: Enhance, Adjust Colour, Adjust Hue/Saturation [and make sure the 'Colorize' box is ticked].
It will take a while to manually edit the entire image ['View, Zoom' to aid tracing], but I doubt any image processing software could fully 'automatically' select the regions you want to colorize, particularly with a greyscale SEM/TEM image. Photoshop's 'Quick selection tool' will have a go though [the tools selection effect is adjustable in the upper menu bar].
I doubt the likes of alternative 'Pseudocolour' LUT effects will provide the subtle colouring you require, although they may work adequately on TEM images of sections.
Hope this helps,
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: 01 January 2012 16:57 To: kjmorris-at-well.ox.ac.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both isha.mutreja-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: How to process SEM images by adobe photoshop to add coloured effects to those images. Thank you in anticipation. Looking forward for some help and guidance.
I tried replying in the thread, but the spam filter thought I had an attachment and refused it, so Keith's instructions are not "below".
************************************************************************* Yes, follow Keith's instructions below. Learn to use your selection tools well, deciding, for example, if you want to feather the selection so it looks more natural. I do like to use Selection - Save Selection so I can save each selection as I go (and not lose all that hard work) and go back and play with them later. Selectin the background first is often easiest, and then you can select the inverse. You can use the Brush tool, the paint bucket, or Edit - Fill, but in each case remember to select Color mode instead of Normal (up on the menu bar). It is not automatic; it is time consuming, but it can also be very therapeutic! These days, instead of maintaining my MicroAngela website I make enamel jewelry under a microscope... Same idea; surround an area with wire and then fill in with colors. Yes, I did like coloring books when I was a child!
Aloha, Tina http://www5.pbrc.hawaii.edu/microangela/
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 21 -- From tina-at-pbrc.hawaii.edu Thu Jan 5 12:22:47 2012 5, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q05IMkJC021637 5, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Jan 2012 12:22:47 -0600 5, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id q05IMipL003272 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 21 -- Thu, 5 Jan 2012 08:22:45 -1000 (HST) 5, 21 -- Received: from localhost (tina-at-localhost) 5, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id q05IMiDs003268; 5, 21 -- Thu, 5 Jan 2012 08:22:44 -1000 (HST) 5, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 5, 21 -- Date: Thu, 5 Jan 2012 08:22:44 -1000 (HST) 5, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 21 -- X-X-Sender: tina-at-b1000 5, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 21 -- cc: isha.mutreja-at-gmail.com 5, 21 -- Subject: Process SEM images by Adobe Photoshop 5, 21 -- Message-ID: {Pine.GSO.4.64.1201050817540.2919-at-b1000} 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Dear Listers Thanks in advance for your time. A researcher here (see below) would like to quench her GFP signal. Any thoughts on how best to quench GFP with out disrupting the other antigens?
"Another question I have is if you known how to quench a GFP signal? I've been researching online and asking people and it seems that there isn't a good consensus on how to do it. I've heard that acetone fixation will work but I'm afraid that it might mess up my other antigens. I can't re-clone my virus, so I'm kind of stuck with the GFP...."
Happy New Year Mary
Microscopy Facility Director Neuroscience Research Institute& Molecular, Cellular& Developmental Biology Biology 2 Building, room 5173 The University of California Santa Barbara, CA 93106-5060
==============================Original Headers============================== 5, 18 -- From mary.raven-at-lifesci.ucsb.edu Thu Jan 5 19:09:55 2012 5, 18 -- Received: from mailsvr.lifesci.ucsb.edu (mailsvr.lifesci.ucsb.edu [128.111.90.44]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0619sB1016160 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 Jan 2012 19:09:55 -0600 5, 18 -- MIME-version: 1.0 5, 18 -- Content-transfer-encoding: 7BIT 5, 18 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 5, 18 -- Received: from [128.111.209.214] (zeus.nri.ucsb.edu [128.111.209.214]) 5, 18 -- by mailsvr.lifesci.ucsb.edu 5, 18 -- (Sun Java(tm) System Messaging Server 7.3-11.01 64bit (built Sep 1 2009)) 5, 18 -- with ESMTPSA id {0LXC00DXIR8I7F00-at-mailsvr.lifesci.ucsb.edu} for 5, 18 -- Microscopy-at-Microscopy.Com; Thu, 05 Jan 2012 17:09:54 -0800 (PST) 5, 18 -- Message-id: {4F0649FA.3020806-at-lifesci.ucsb.edu} 5, 18 -- Date: Thu, 05 Jan 2012 17:10:18 -0800 5, 18 -- From: Mary Raven {mary.raven-at-lifesci.ucsb.edu} 5, 18 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:8.0) Gecko/20111105 Thunderbird/8.0 5, 18 -- To: Microscopy-at-Microscopy.Com 5, 18 -- Subject: LM - how to quench a GFP signal ==============================End of - Headers==============================
Recently I've been asked a question about upper magnification range with the SEM. I know upper magnification is going to depend on each SEM, type of filament, skill of the operator, sample type, definitions of what's useful and other factors I haven't even thought of. But, as a general rule of thumb, what's the normal upper magnification with meaningful resolution (Definition: I can clearly see the features I want and would publish the image) for a ordinary hair pin filament SEM?
any thoughts?
Thanks!!!!
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 8, 25 -- From frank_karl-at-ardl.com Fri Jan 6 07:58:43 2012 8, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q06DwgKm014027 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2012 07:58:43 -0600 8, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 8, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 6 Jan 8, 25 -- 2012 08:58:41 -0500 8, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 8, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 8, 25 -- Date: Fri, 6 Jan 2012 08:58:41 -0500 8, 25 -- Subject: Upper magnification 8, 25 -- Thread-Topic: Upper magnification 8, 25 -- Thread-Index: AczMe0wDdrmKsafpShmJ5rlMh9GWEQ== 8, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150770155C70B28-at-exchange2k7.ad.ardl.com} 8, 25 -- Accept-Language: en-US 8, 25 -- Content-Language: en-US 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- acceptlanguage: en-US 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q06DwgKm014027 ==============================End of - Headers==============================
The Company: Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries.
The Opportunity: The company currently has an opening for a Marketing Manager - Life Science and Clinical to be based in Buffalo Grove/Chicago IL. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary.
Primary Responsibilities: This role will be responsible for developing and building unique value propositions that support the go to market strategy of the business. The Marketing Manager will integrate and coordinate all functional activities that are necessary to achieve and maintain sales and profitability within the defined Market Segment.
If you meet and/or exceed the experience criteria, please visit our website link (by clicking on link or by Copy and Paste) for the full job description and to apply directly. We wish everyone the best of luck. Unfortunately only qualified candidates will be considered.
Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries.
The Opportunity:
The company currently has an opening for a Sales Representative - Nanotechnology ideally based in Wetzlar, Germany. All applicants must not be adverse to travel, as this is a position that will require travel within the position territory.
Salary: 40,000 - 50,000 EUR plus bonus of 15,000 EUR
Other: Company Car - Pension Plan - Home Office - Laptop - Mobile Phone - Internet paid by company
Primary Responsibilities:
- Direct sales of products for sample preparation for electron microscopy in territory - Acquisition of new customers - Active support and consulting of the existing customers in industry and research - Presentation of preparation systems during trade shows, exhibitions, workshops and customer demonstrations - Budget responsibility and autonomous implementation of sales strategies
Education and Experience Required:
- University degree in natural science or comparable education - Preferably 3 to 4 years of experience in sales of capital equipment - Preferably job experience in the field of electron microscopy - Ability to travel extensively and work independently - Good command of English and excellent communications and negotiation skills
If you meet and/or exceed the experience criteria, please submit your resume by clicking on the link below or Copy and Paste link into browser. We wish everyone the best of luck. Unfortunately only qualified candidates will be considered.
http://tinyurl.com/Micro-SalesNano-Germany
Christy Edwards Sr. e-Recruitment Consultant Personify 5020 Weston Parkway Suite 315 Cary, North Carolina 27513 www.personifysearch.com Email: ce-at-personifysearch.com
==============================Original Headers============================== 15, 19 -- From ce-at-personifysearch.com Fri Jan 6 10:48:51 2012 15, 19 -- Received: from na3sys010aog102.obsmtp.com (na3sys010aog102.obsmtp.com [74.125.245.72]) 15, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q06GmoPa018958 15, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Jan 2012 10:48:50 -0600 15, 19 -- Received: from mail-wi0-f177.google.com ([209.85.212.177]) (using TLSv1) by na3sys010aob102.postini.com ([74.125.244.12]) with SMTP 15, 19 -- ID DSNKTwcl8ji17W3gMZ6+acKrEsHx1dUkW4Ax-at-postini.com; Fri, 06 Jan 2012 08:48:50 PST 15, 19 -- Received: by mail-wi0-f177.google.com with SMTP id hq15so1712252wib.8 15, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 06 Jan 2012 08:48:49 -0800 (PST) 15, 19 -- Received: by 10.180.81.72 with SMTP id y8mr12699694wix.14.1325868529764; Fri, 15, 19 -- 06 Jan 2012 08:48:49 -0800 (PST) 15, 19 -- From: Christy Edwards {ce-at-personifysearch.com} 15, 19 -- MIME-Version: 1.0 15, 19 -- X-Mailer: Microsoft Office Outlook 12.0 15, 19 -- Thread-Index: AczMkxElH1peicrWQAivXjb7tvW9/g== 15, 19 -- Date: Fri, 6 Jan 2012 11:48:50 -0500 15, 19 -- Message-ID: {a665e51b2dab324a43accec9dcb6a46b-at-mail.gmail.com} 15, 19 -- Subject: Nanotechnology Sales Opportunity in Germany 15, 19 -- To: Microscopy-at-microscopy.com 15, 19 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Having taken about 20,000 biological SEM photos with a tungsten filament SEM, the useful top magnification for images of the surface of cells runs about 20,000x. You can push this to as much as 100,000x for organelles of ideally prepared samples if your instrument can go to 35-40kV and you are working with gold-palladium coated samples. I've imaged viruses budding from the surface of cells at 20-40kX before by SEM and had good photos. With material samples such as metals, where you have less beam penetration and more signal, you will get better signal to noise ratio and better high-end magnification photos, so 100,000x photos are not much of a problem. My microscope maxed out at 180,000x, and I could get grainy photos of my resolution sample at that magnification, but could still resolve 5nm on a tin ball on carbon sample.
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: ISA 1046 ________________________________________ X-from: frank_karl-at-ardl.com [frank_karl-at-ardl.com] Sent: Friday, January 06, 2012 9:08 AM To: Haller, Edward
Recently I've been asked a question about upper magnification range with the SEM. I know upper magnification is going to depend on each SEM, type of filament, skill of the operator, sample type, definitions of what's useful and other factors I haven't even thought of. But, as a general rule of thumb, what's the normal upper magnification with meaningful resolution (Definition: I can clearly see the features I want and would publish the image) for a ordinary hair pin filament SEM?
any thoughts?
Thanks!!!!
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 8, 25 -- From frank_karl-at-ardl.com Fri Jan 6 07:58:43 2012 8, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q06DwgKm014027 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2012 07:58:43 -0600 8, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 8, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 6 Jan 8, 25 -- 2012 08:58:41 -0500 8, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 8, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 8, 25 -- Date: Fri, 6 Jan 2012 08:58:41 -0500 8, 25 -- Subject: Upper magnification 8, 25 -- Thread-Topic: Upper magnification 8, 25 -- Thread-Index: AczMe0wDdrmKsafpShmJ5rlMh9GWEQ== 8, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150770155C70B28-at-exchange2k7.ad.ardl.com} 8, 25 -- Accept-Language: en-US 8, 25 -- Content-Language: en-US 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- acceptlanguage: en-US 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q06DwgKm014027 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 42 -- From ehaller-at-health.usf.edu Fri Jan 6 11:12:58 2012 15, 42 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 15, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q06HCwEj003820 15, 42 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Jan 2012 11:12:58 -0600 15, 42 -- X-ASG-Debug-ID: 1325869977-045fa804554d2f10001-1DjkGe 15, 42 -- Received: from EXCH01.hscnet.hsc.usf.edu ([10.119.4.41]) by hscantispam.health.usf.edu with ESMTP id RLQBvgiDZCTMs958; Fri, 06 Jan 2012 12:12:57 -0500 (EST) 15, 42 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 15, 42 -- Received: from EXCH03.hscnet.hsc.usf.edu ([fe80::94db:1b22:fb18:d0b4]) by 15, 42 -- EXCH01.hscnet.hsc.usf.edu ([10.119.4.41]) with mapi id 14.01.0339.001; Fri, 6 15, 42 -- Jan 2012 12:12:56 -0500 15, 42 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 15, 42 -- X-Barracuda-Apparent-Source-IP: fe80::94db:1b22:fb18:d0b4 15, 42 -- To: "frank_karl-at-ardl.com" {frank_karl-at-ardl.com} , 15, 42 -- "Microscopy-at-microscopy.com" 15, 42 -- {Microscopy-at-microscopy.com} 15, 42 -- Subject: RE: [Microscopy] Upper magnification 15, 42 -- Thread-Topic: [Microscopy] Upper magnification 15, 42 -- X-ASG-Orig-Subj: RE: [Microscopy] Upper magnification 15, 42 -- Thread-Index: AQHMzHye7uF0om+kfEupT4+D7Yc1eJX/kElZ 15, 42 -- Date: Fri, 6 Jan 2012 17:12:56 +0000 15, 42 -- Message-ID: {89AF5EEF4C4B8943A8D091D28859F7C84B157CEB-at-EXCH03.hscnet.hsc.usf.edu} 15, 42 -- References: {201201061408.q06E87C4025430-at-ns.microscopy.com} 15, 42 -- In-Reply-To: {201201061408.q06E87C4025430-at-ns.microscopy.com} 15, 42 -- Accept-Language: en-US 15, 42 -- Content-Language: en-US 15, 42 -- X-MS-Has-Attach: 15, 42 -- X-MS-TNEF-Correlator: 15, 42 -- x-originating-ip: [10.119.4.53] 15, 42 -- Content-Type: text/plain; charset="us-ascii" 15, 42 -- MIME-Version: 1.0 15, 42 -- X-Barracuda-Connect: UNKNOWN[10.119.4.41] 15, 42 -- X-Barracuda-Start-Time: 1325869977 15, 42 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-mod/mark.cgi 15, 42 -- X-Virus-Scanned: by bsmtpd at health.usf.edu 15, 42 -- X-Barracuda-Spam-Score: 0.00 15, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 15, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.85191 15, 42 -- Rule breakdown below 15, 42 -- pts rule name description 15, 42 -- ---- ---------------------- -------------------------------------------------- 15, 42 -- Content-Transfer-Encoding: 8bit 15, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q06HCwEj003820 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nesmicroscopy-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: nesmicroscopy-at-gmail.com Name: NESM
Organization: The New England Society for Microscopy
Title-Subject: [Filtered] SAVE-THE-DATE: NESM February Meeting -at- Saint-Gobain
Message: Greetings Confocal Listserv-ites,
This is your friendly SAVE-THE-DATE reminder for NESM's February Dinner Meeting hosted by Saint-Gobain on February 15, 2012 -at- 5:30PM. The meeting is composed of a Saint-Gobain facility tour, dinner, and two technical talks (see below for talk information). Stay tuned for detailed information about the February Meeting in the coming week. You can also keep up with NESM activities by checking us out of Facebook (https://www.facebook.com/pages/NESM/149251225154665) and Twitter (http://twitter.com/#!/NESMicroscopy).
Technical Talks:
"Crystals as Stacked Layers and the Infinite World of Intergrowths", Charles Bateman, Ph.D., Saint-Gobain, Northboro, MA
"Microscopy tools: visual resolution of insect eyes", Paloma Gonzalez Bellido, Ph.D., Marine Biological Laboratory, Woods Hole, MA
As with the SEM of Ed Haller, our 28 y/o Hitachi S570 gave reasonably good images up to 100KX. I believe this is probably a good expectation for most tungsten-filament based SEMs. Some might give even better, while others (like entry level or variable pressure instruments) may not perform as well.
Of course, trimming the SEM (alignment, stigmation, good vacuum, stable and conductive specimen) will have a tremendous effect on the final image.
Cheers, -- John J. Bozzola, Ph.D., Professor & Director of IMAGE (Retired : -) Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 4, 20 -- From bozzola-at-siu.edu Sat Jan 7 09:30:37 2012 4, 20 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q07FUbvs004751 4, 20 -- for {Microscopy-at-microscopy.com} ; Sat, 7 Jan 2012 09:30:37 -0600 4, 20 -- Received: by vcge1 with SMTP id e1so2107012vcg.0 4, 20 -- for {Microscopy-at-microscopy.com} ; Sat, 07 Jan 2012 07:30:37 -0800 (PST) 4, 20 -- MIME-Version: 1.0 4, 20 -- Received: by 10.220.107.73 with SMTP id a9mr5930442vcp.11.1325950236916; Sat, 4, 20 -- 07 Jan 2012 07:30:36 -0800 (PST) 4, 20 -- Received: by 10.52.38.232 with HTTP; Sat, 7 Jan 2012 07:30:36 -0800 (PST) 4, 20 -- In-Reply-To: {201201061713.q06HDxHV004949-at-ns.microscopy.com} 4, 20 -- References: {201201061713.q06HDxHV004949-at-ns.microscopy.com} 4, 20 -- Date: Sat, 7 Jan 2012 09:30:36 -0600 4, 20 -- Message-ID: {CAKeCJ9wAG=4TUy9eYy__iK5hMch1VQZK24Q-pUyrFb8SBmQe9w-at-mail.gmail.com} 4, 20 -- Subject: Re: [Microscopy] RE: Upper magnification 4, 20 -- From: John Bozzola {bozzola-at-siu.edu} 4, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 4, 20 -- Content-Type: text/plain; charset=UTF-8 4, 20 -- Content-Transfer-Encoding: 8bit 4, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q07FUbvs004751 ==============================End of - Headers==============================
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************************** } Date: Mon, 9 Jan 2012 12:02:29 -0800 } Reply-To: Venu Polineni {vens06-at-gmail.com} } Subject: Ask-A-Microscopist } } realname - Venu Polineni } Email - vens06-at-gmail.com } EDUCATION - Graduate College } SUBJECT_OF_QUESTION - FRET } QUESTION - Is there a specific chemical like saponin to intensify a } FRET signal? Or are there specific agents/detergents/fixatives that } either intensify or bleach a FRET signal. } --
==============================Original Headers============================== 1, 24 -- From oshel1pe-at-cmich.edu Mon Jan 9 14:09:16 2012 1, 24 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q09K9GP4027775 1, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Jan 2012 14:09:16 -0600 1, 24 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 1, 24 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q09K9F8j015150 1, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Jan 2012 15:09:15 -0500 1, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 1, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Mon, 9 Jan 1, 24 -- 2012 15:09:15 -0500 1, 24 -- MIME-Version: 1.0 1, 24 -- Message-ID: {a0624080dcb30f99e1935-at-[141.209.160.249]} 1, 24 -- Date: Mon, 9 Jan 2012 15:09:12 -0500 1, 24 -- To: {Microscopy-at-microscopy.com} 1, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 24 -- Subject: intensifying a FRET signal 1, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 1, 24 -- X-Originating-IP: [141.209.160.249] 1, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) 1, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 1, 24 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 24 -- X-CanItPRO-Stream: default 1, 24 -- X-Canit-Stats-ID: 06Gjw9fVQ - 5a0ef6462510 - 20120109 1, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Rodighiero, S., et al., Fixation, mounting and sealing with nail polish of cell specimens lead to incorrect FRET measurements using acceptor photobleaching. Cellular Physiology and Biochemistry, 2008. 21(5-6): p. 489-498.
John Oreopoulos Research Assistant Spectral Applied Research Richmond Hill, Ontario Canada www.spectral.ca
On 2012-01-09, at 3:15 PM, oshel1pe-at-cmich.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *************************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } **************************************************************************************** } } Date: Mon, 9 Jan 2012 12:02:29 -0800 } } Reply-To: Venu Polineni {vens06-at-gmail.com} } } Subject: Ask-A-Microscopist } } } } realname - Venu Polineni } } Email - vens06-at-gmail.com } } EDUCATION - Graduate College } } SUBJECT_OF_QUESTION - FRET } } QUESTION - Is there a specific chemical like saponin to intensify a } } FRET signal? Or are there specific agents/detergents/fixatives that } } either intensify or bleach a FRET signal. } } } -- } } ==============================Original Headers============================== } 1, 24 -- From oshel1pe-at-cmich.edu Mon Jan 9 14:09:16 2012 } 1, 24 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) } 1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q09K9GP4027775 } 1, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Jan 2012 14:09:16 -0600 } 1, 24 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) } 1, 24 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q09K9F8j015150 } 1, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Jan 2012 15:09:15 -0500 } 1, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local } 1, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Mon, 9 Jan } 1, 24 -- 2012 15:09:15 -0500 } 1, 24 -- MIME-Version: 1.0 } 1, 24 -- Message-ID: {a0624080dcb30f99e1935-at-[141.209.160.249]} } 1, 24 -- Date: Mon, 9 Jan 2012 15:09:12 -0500 } 1, 24 -- To: {Microscopy-at-microscopy.com} } 1, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } 1, 24 -- Subject: intensifying a FRET signal } 1, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 1, 24 -- X-Originating-IP: [141.209.160.249] } 1, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) } 1, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) } 1, 24 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 } 1, 24 -- X-CanItPRO-Stream: default } 1, 24 -- X-Canit-Stats-ID: 06Gjw9fVQ - 5a0ef6462510 - 20120109 } 1, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 } ==============================End of - Headers==============================
==============================Original Headers============================== 14, 28 -- From john.oreopoulos-at-utoronto.ca Mon Jan 9 14:31:12 2012 14, 28 -- Received: from bureau81.ns.utoronto.ca (bureau81.ns.utoronto.ca [128.100.132.181]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q09KVCk0012671 14, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Jan 2012 14:31:12 -0600 14, 28 -- Received: from [192.168.1.218] (smtp.spectral.ca [64.40.242.117]) 14, 28 -- (authenticated bits=0) 14, 28 -- by bureau81.ns.utoronto.ca (8.13.8/8.13.8) with ESMTP id q09KUIr7024863 14, 28 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 14, 28 -- Mon, 9 Jan 2012 15:30:21 -0500 14, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/simple; d=utoronto.ca; s=beta; 14, 28 -- t=1326141022; bh=Cq6z7LPbMONMOxhPZ1mXb0AFoMvwMJpLl46GBFgFQps=; 14, 28 -- h=Content-Type:Mime-Version:Subject:From:In-Reply-To:Date: 14, 28 -- Content-Transfer-Encoding:Message-Id:References:To; 14, 28 -- b=M1ucKS5wmPzQVPiF28bNBYY8kiG+BdzZJLwIH2N2Zx80AQlFRjhkirc9mG/j9cRil 14, 28 -- 3QpYK0B8NrmuYcOYKZbovtPs0I5/NaixivF/V7ewGDRt/jGMmG8P15FSESNFI5wppP 14, 28 -- 8ERDk+h8jIGG4vputLon7L8cssCmCpcyDcRH6SC4= 14, 28 -- Content-Type: text/plain; charset=us-ascii 14, 28 -- Mime-Version: 1.0 (Apple Message framework v1084) 14, 28 -- Subject: Re: [Microscopy] intensifying a FRET signal 14, 28 -- From: John Oreopoulos {john.oreopoulos-at-utoronto.ca} 14, 28 -- In-Reply-To: {201201092015.q09KFLx0002314-at-ns.microscopy.com} 14, 28 -- Date: Mon, 9 Jan 2012 15:30:18 -0500 14, 28 -- Message-Id: {2A831DF1-BBB0-4D5E-8452-955E5377F6A8-at-utoronto.ca} 14, 28 -- References: {201201092015.q09KFLx0002314-at-ns.microscopy.com} 14, 28 -- To: oshel1pe-at-cmich.edu, Microscopy-at-microscopy.com 14, 28 -- X-Mailer: Apple Mail (2.1084) 14, 28 -- Content-Transfer-Encoding: 8bit 14, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q09KVCk0012671 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both yamawaki-at-stanford.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Anti-static medicine cups
Message: We used polypropylene specimen cups (static-free) to prepare the water in the boats for sectioning. They made water static-free. Our serial sections did not move around the boat.
Now these cups do not work. There has been a change in the manufacturing of the cups so they no longer have the ability to make our water static free.
Has anyone else come up with a way to solve this problem?
Thank you, Ruth Yamawaki Stanford University Department of Comparative Medicine 650-723-3457
I have a colleague at Creighton University who is trying to repair a LKB-Pyramitome model 11800 and needs a "toothed" belt. Can anyone help? She doesn't subscribe to this listserver so I'll pass along any information to her. Thanks.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 2, 23 -- From tbargar-at-unmc.edu Wed Jan 11 09:15:22 2012 2, 23 -- Received: from zixvpm01.unmc.edu (mysecuremail.unmc.edu [192.198.54.126] (may be forged)) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0BFFLi5025938 2, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 09:15:21 -0600 2, 23 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) 2, 23 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 5BCE81090492 2, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 09:15:21 -0600 (CST) 2, 23 -- Received: from unmcnotes01.unmc.edu (unknown [10.8.51.5]) 2, 23 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id 27E301090491 2, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 09:15:20 -0600 (CST) 2, 23 -- To: Microscopy-at-microscopy.com 2, 23 -- MIME-Version: 1.0 2, 23 -- Subject: spare parts for LKB-Pyramitome model 11800 2, 23 -- X-KeepSent: 2B38D32F:4F2DEA13-86257982:00534294; 2, 23 -- type=4; name=$KeepSent 2, 23 -- X-Mailer: Lotus Notes Release 8.0.2 August 07, 2008 2, 23 -- Message-ID: {OF2B38D32F.4F2DEA13-ON86257982.00534294-86257982.0053CD5C-at-unmc.edu} 2, 23 -- From: Tom W Bargar {tbargar-at-unmc.edu} 2, 23 -- Date: Wed, 11 Jan 2012 09:15:20 -0600 2, 23 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/11/2012 09:15:20 2, 23 -- AM, 2, 23 -- Serialize complete at 01/11/2012 09:15:20 AM 2, 23 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
I am not familiar with the particular model, but if dimensions of the belt can be measured or deducted from the design of system then good places to start looking for it may be:
In unlikely case that McMaster and SmallParts would not have what you need - try to Google for "timing belt", I am sure that there are plenty of other places to get it.
Cheers :) Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 1/11/2012 10:16 AM, tbargar-at-unmc.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have a colleague at Creighton University who is trying to repair a } LKB-Pyramitome model 11800 and needs a "toothed" belt. Can anyone help? } She doesn't subscribe to this listserver so I'll pass along any } information to her. Thanks. } } Tom Bargar } University of Nebraska Medical Center } Core Electron Microscopy Research Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } ==============================Original Headers============================== } 2, 23 -- From tbargar-at-unmc.edu Wed Jan 11 09:15:22 2012 } 2, 23 -- Received: from zixvpm01.unmc.edu (mysecuremail.unmc.edu [192.198.54.126] (may be forged)) } 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0BFFLi5025938 } 2, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 09:15:21 -0600 } 2, 23 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) } 2, 23 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 5BCE81090492 } 2, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 09:15:21 -0600 (CST) } 2, 23 -- Received: from unmcnotes01.unmc.edu (unknown [10.8.51.5]) } 2, 23 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id 27E301090491 } 2, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 09:15:20 -0600 (CST) } 2, 23 -- To: Microscopy-at-microscopy.com } 2, 23 -- MIME-Version: 1.0 } 2, 23 -- Subject: spare parts for LKB-Pyramitome model 11800 } 2, 23 -- X-KeepSent: 2B38D32F:4F2DEA13-86257982:00534294; } 2, 23 -- type=4; name=$KeepSent } 2, 23 -- X-Mailer: Lotus Notes Release 8.0.2 August 07, 2008 } 2, 23 -- Message-ID: {OF2B38D32F.4F2DEA13-ON86257982.00534294-86257982.0053CD5C-at-unmc.edu} } 2, 23 -- From: Tom W Bargar {tbargar-at-unmc.edu} } 2, 23 -- Date: Wed, 11 Jan 2012 09:15:20 -0600 } 2, 23 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/11/2012 09:15:20 } 2, 23 -- AM, } 2, 23 -- Serialize complete at 01/11/2012 09:15:20 AM } 2, 23 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 43 -- From vray-at-partbeamsystech.com Wed Jan 11 10:51:06 2012 7, 43 -- Received: from nm2-vm3.bullet.mail.ne1.yahoo.com (nm2-vm3.bullet.mail.ne1.yahoo.com [98.138.91.132]) 7, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q0BGp6V1012233 7, 43 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 10:51:06 -0600 7, 43 -- Received: from [98.138.90.48] by nm2.bullet.mail.ne1.yahoo.com with NNFMP; 11 Jan 2012 16:51:05 -0000 7, 43 -- Received: from [98.138.86.157] by tm1.bullet.mail.ne1.yahoo.com with NNFMP; 11 Jan 2012 16:51:05 -0000 7, 43 -- Received: from [127.0.0.1] by omp1015.mail.ne1.yahoo.com with NNFMP; 11 Jan 2012 16:51:05 -0000 7, 43 -- X-Yahoo-Newman-Id: 665484.29560.bm-at-omp1015.mail.ne1.yahoo.com 7, 43 -- Received: (qmail 54525 invoked from network); 11 Jan 2012 16:51:05 -0000 7, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1326300665; bh=c2zwoQrAIHBJZ6Pojcq+4hTsTzBdKcdBNNfQmYWuGcA=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=ziU49Pb0mhNKsBCbKroiPyD+IMBw+3vn9Wp3FyKP6OFGFnbuYtsT+uIvtpTpqmWIwrIKitSpBzm8Ftqd5v7gWAXP9K5NLP3rnJ+ubUEJ5kXQ1rTXTa6YPHuKyxUP4pi6LTU/+YlFNPDanxl8UDF0ebBhLZrCQeYfTAGn4qywFfs= 7, 43 -- X-Yahoo-Newman-Property: ymail-3 7, 43 -- X-YMail-OSG: RTWVLQoVM1mZ04EiyEavV9PLf5FEphbW5s74clx9xib9.7Z 7, 43 -- qky1I9BEhR4qVOZOYgwxWAdOPkmO3yVDPPTGtNWfBV4h2Z_iK_q858T.zpg6 7, 43 -- rc3.Q.ahSdsjR7VeewU38GQWPbLc9Ve7_65MCv.fCZYFXWrMJgs7zHflojEQ 7, 43 -- RQWd2ACElrCNmosGe97RLExH8ojUvnZpVsAmWlCjxNsePVHtHnWSnbTKzH7S 7, 43 -- UfVqJI85g_9pNaUEKN8HGByof.P3RWAKmwe0Hbm1nZR03rHnqfORIy8BWiZc 7, 43 -- wFpL3kX4ioSq5uGoRyiOMRINS.Dpt60EbKjP1SIU36FhCPanoAOGhshoNayI 7, 43 -- pR_GzWKxmOdryZu2Ve5uF0zyW98RDec7PKehjCdGwEigSsPpa3cA_hMJdKge 7, 43 -- sbjRcecHusiblwV2NAedoZw7TSfV1it5T9_CbAgOe.cx7w5t0NlIK9.lk2VM 7, 43 -- JJuw9wow_qXvPArUYAI44Xtf3BsZtWOlTuoObTez2bO0g8VQbdDMGE.NN5Ej 7, 43 -- hgb8ZvVZJ.jHXUeL.q0U3dIeNAdY67o1qMKfDN7wRiFpgXr0loxPx0IpI9tD 7, 43 -- 1cHDzhK3nH2S3cByou39vegn4ENsVuFZ6j4ss9DUE.L7kbdfpytDxUGqqErm 7, 43 -- 885UwkJQJTTPjOrn1d8qVeTbyH1j1abbON.Ep1IeuwXOdi5E99JlJsxy9BkE 7, 43 -- 6.K0Qch10Z11Ttx_nAmt_T7ib2U_WNJglAyIFL5JmqsEM25ESF7Ec_JkxzAP 7, 43 -- qqEkyVoL0PnG.c7HcPSEkydLCFnwRln8zoaJYHaZ8qLPgVojSvZ_15JSycLo 7, 43 -- DMbm31VxW9v_bpw-- 7, 43 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 43 -- Received: from [192.168.2.6] (vray-at-96.233.126.242 with plain) 7, 43 -- by smtp112.biz.mail.mud.yahoo.com with SMTP; 11 Jan 2012 08:51:05 -0800 PST 7, 43 -- Message-ID: {4F0DBDF8.4020404-at-partbeamsystech.com} 7, 43 -- Date: Wed, 11 Jan 2012 11:51:04 -0500 7, 43 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 7, 43 -- Reply-To: vray-at-partbeamsystech.com 7, 43 -- Organization: PBS&T 7, 43 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:8.0) Gecko/20111105 Thunderbird/8.0 7, 43 -- MIME-Version: 1.0 7, 43 -- To: tbargar-at-unmc.edu 7, 43 -- CC: microscopy-at-microscopy.com 7, 43 -- Subject: Re: [Microscopy] spare parts for LKB-Pyramitome model 11800 7, 43 -- References: {201201111516.q0BFGI11027233-at-ns.microscopy.com} 7, 43 -- In-Reply-To: {201201111516.q0BFGI11027233-at-ns.microscopy.com} 7, 43 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 43 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
-- I know that most EM labs have moved away from EM film although quite a few labs in my field still use it. I just got an email from my vendor who gave me an update on Kodak film. I am posting that message in its entirety. I thought that a number of other users on the list might like this information so I hope I am not crossing the line into advertising.
Norm Olson University of California San Diego *********************** Norm-
Good Afternoon Below is an email from my Kodak Product Manager about the Kodak SO-163 EM film you purchase from us. I have had a number of calls pertaining to the film in the last few days since Kodak announced their probability of filing bankruptcy. Please don't hesitate to call or email me if there are any additional questions you may have. I have stock available if you need to place an order. Best Regards, Sheila
KODAK Electron Microscope 4489 and KODAK Electron Image Film SO-163 are manufactured and sold by Carestream Molecular Imaging. You can assure your customers that these products will be readily available in the future as far as we can see.
Some background information. When Eastman Kodak Company spun off the health sciences part of their business which became Carestream Health in 2007, Carestream Health purchased all the film factories and manufacturing equipment as well as retaining all the skilled staff to continue producing the film products. Based on the value of the Kodak name, Carestream has an agreement with Eastman Kodak Company to sell the product under the Kodak name.
The film is made on the same machines by the same talented manufacturing teams by Carestream Health. You can inform customers you have spoken directly with the film source company and the EM film is and will be available for them whenever they need it.
Please do not hesitate to contact me if I can be of assistance on any matter.
Best regards, Eric
Eric Ambrose Product Manager - Media Products Carestream Molecular Imaging
eric.ambrose-at-carestream.com P 585-627-8762 M 585-330-1646
Carestream Health, Inc. 150 Verona Street Rochester, NY 14608
==============================Original Headers============================== 22, 33 -- From nholson-at-ucsd.edu Wed Jan 11 15:39:25 2012 22, 33 -- Received: from iport-c2-out.ucsd.edu (iport-c2-out.ucsd.edu [132.239.0.119]) 22, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0BLdOhL004669 22, 33 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 15:39:24 -0600 22, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 22, 33 -- d=ucsd.edu; i=nholson-at-ucsd.edu; q=dns/txt; 22, 33 -- s=041709-iport; t=1326317965; x=1357853965; 22, 33 -- h=mime-version:message-id:date:to:from:subject; 22, 33 -- bh=ty0v7GKSzT611lI+oR2v1as2KLGrFjmw5Zv5qYggYpA=; 22, 33 -- b=pkmfaPIwcQ4b0JqXWs0dDqdCtvGWnAOVzsA6k/28Lie6QmFTrgHthPG0 22, 33 -- Arfv1UFv0X82/vobYWSrua+NHB/oc5l0lu8du4ZHq9Af36zyxyb26Sksb 22, 33 -- 9b9riCYGNq6hHiN8qoIJgmkSdpyUIiL/wsbIjLyP0qvnuBJKRqRR5ods4 22, 33 -- E=; 22, 33 -- X-IronPort-Anti-Spam-Filtered: true 22, 33 -- X-IronPort-Anti-Spam-Result: ArAHAKEADk+E7/kU/2dsb2JhbABAA50EAZABgQWCMw8+QytVn2eWb4kFBIh4BYMcBIg6khATjQw 22, 33 -- X-IronPort-AV: E=Sophos;i="4.71,494,1320652800"; 22, 33 -- d="scan'208";a="694508023" 22, 33 -- X-Spam-Status: No 22, 33 -- X-Spam-Level: 22, 33 -- Received: from smtp-tpcs.ucsd.edu ([132.239.249.20]) 22, 33 -- by iport-c2-out.ucsd.edu with ESMTP; 11 Jan 2012 13:39:23 -0800 22, 33 -- Received: from [132.239.70.186] (patrick-70-186.ucsd.edu [132.239.70.186]) 22, 33 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 22, 33 -- (No client certificate requested) 22, 33 -- by smtp-tpcs.ucsd.edu (Postfix) with ESMTPSA id D6970806C6 22, 33 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 13:39:20 -0800 (PST) 22, 33 -- Mime-Version: 1.0 22, 33 -- Message-Id: {p06240872cb33b09d114d-at-[132.239.70.186]} 22, 33 -- Date: Wed, 11 Jan 2012 13:39:21 -0800 22, 33 -- To: microscopy-at-microscopy.com 22, 33 -- From: Norm Olson {nholson-at-ucsd.edu} 22, 33 -- Subject: Availability of Kodak EM film 22, 33 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
If the belt can be measured, here are two sources to check: http://www.smallparts.com or http://www.pic-design.com
Woody } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have a colleague at Creighton University who is trying to repair a } LKB-Pyramitome model 11800 and needs a "toothed" belt. Can anyone help? } She doesn't subscribe to this listserver so I'll pass along any } information to her. Thanks. } }
--
==============================Original Headers============================== 5, 35 -- From SRS0=OxudxM=7W=albe24.com=woody-at-eigbox.net Wed Jan 11 16:44:39 2012 5, 35 -- Received: from bosmailout01.eigbox.net (bosmailout01.eigbox.net [66.96.186.1]) 5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0BMidZM022373 5, 35 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 16:44:39 -0600 5, 35 -- Received: from bosmailscan20.eigbox.net ([10.20.15.20]) 5, 35 -- by bosmailout01.eigbox.net with esmtp (Exim) 5, 35 -- id 1Rl6uM-0003vA-G6 5, 35 -- for microscopy-at-microscopy.com; Wed, 11 Jan 2012 17:44:38 -0500 5, 35 -- Received: from bosimpout01.eigbox.net ([10.20.55.1]) 5, 35 -- by bosmailscan20.eigbox.net with esmtp (Exim) 5, 35 -- id 1Rl6uM-0002TE-Dg; Wed, 11 Jan 2012 17:44:38 -0500 5, 35 -- Received: from bosauthsmtp13.eigbox.net ([10.20.18.13]) 5, 35 -- by bosimpout01.eigbox.net with NO UCE 5, 35 -- id LNke1i00C0GvDVm01NkeXk; Wed, 11 Jan 2012 17:44:38 -0500 5, 35 -- X-EN-OrigOutIP: 10.20.18.13 5, 35 -- X-EN-IMPSID: LNke1i00C0GvDVm01NkeXk 5, 35 -- Received: from 110.sub-97-21-63.myvzw.com ([97.21.63.110] helo=[192.168.0.199]) 5, 35 -- by bosauthsmtp13.eigbox.net with esmtpa (Exim) 5, 35 -- id 1Rl6uM-0007xj-7G; Wed, 11 Jan 2012 17:44:38 -0500 5, 35 -- Message-ID: {4F0E3B4E.9020100-at-albe24.com} 5, 35 -- Date: Wed, 11 Jan 2012 17:45:50 -0800 5, 35 -- From: woody {woody-at-albe24.com} 5, 35 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.25) Gecko/20111213 Lightning/1.0b2 Thunderbird/3.1.17 5, 35 -- MIME-Version: 1.0 5, 35 -- To: tbargar-at-unmc.edu, Microscopy-at-microscopy.com 5, 35 -- Subject: Re: [Microscopy] spare parts for LKB-Pyramitome model 11800 5, 35 -- References: {201201111524.q0BFOm5J008433-at-ns.microscopy.com} 5, 35 -- In-Reply-To: {201201111524.q0BFOm5J008433-at-ns.microscopy.com} 5, 35 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 35 -- Content-Transfer-Encoding: 7bit 5, 35 -- X-EN-UserInfo: cb2fc58be0a538172161d6e314253eda:05dddf68a676b03081895b01609fb24b 5, 35 -- X-EN-AuthUser: woody-at-albe24.com 5, 35 -- Sender: woody {woody-at-albe24.com} 5, 35 -- X-EN-OrigIP: 97.21.63.110 5, 35 -- X-EN-OrigHost: 110.sub-97-21-63.myvzw.com ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ranjani.ganji-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mmh-at-umn.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mmh-at-umn.edu Name: Marc Hirschmann
Organization: University of Minnesota
Title-Subject: [Filtered] Job Opportunity: Lab Mgr. for electron microprobe lab
Message: Electron Microprobe Research Scientist, University of Minnesota, Minneapolis The Department of Earth Sciences is seeking applications for an Electron Microprobe Research Scientist. Responsibilities will include operation, supervision and routine maintenance of a 5-spectrometer JEOL 8900 electron microprobe. Experience analyzing geological samples by EMPA is essential. Some experience maintaining a microprobe is desirable, but some amount of on-the-job training is expected and the instrument continues to be on a JEOL service contract. The successful applicant will also be expected to provide user training, including teaching a course in microprobe theory and applications, and to consult/collaborate with University researchers and students on research projects. Additionally, the position requires providing support for a moderate load of industrial users. The position also provides opportunities to conduct independent research using the microprobe as well as other facilities available at UMN. An M.S. or Ph.D. degree in Earth Science or related field is required. This non-tenure track position is available as a Research Fellow (M.S. degree, requisition #172657), or a Postdoctoral Associate (up to 3 years experience post PhD, requisition #172658), or a Research Associate (3 years post PhD experience, requisition #175602). To apply, candidates should go to https://employment.umn.edu and attach a cover letter, resume (CV), statement of research interests, and names/contact information of three references. Application review will begin on March 15, 2012, and will continue until the position is filled. For further information, contact Marc Hirschmann, mmh-at-umn.edu.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mchimento-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?
Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They introduce the NDs before polymerization at room temperature. They said that it forms a “brick” of polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if they could check dispersion of the ND in the polymer with it??
sectioning a polymer is not really a big deal if you have a (cryo)- microtome. However, as always the devil is in the details! First you need to know the TG (Glasstransition Temperature) of the polymer in question. If it´s below RT you should prepare to do some cryo cutting. If not, so much the better. The real problem will be the ND, they will surely damage your knife and will be bunked out of the polymer matirix to some extent. However, you can try using a glass knife or I would suggest to use an used diamond knife, where you don´t mind a few more additional scratches. Just give it a try, it´s not that difficult if you know how to operate a microtome.
Cheers Ingo } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } mchimento-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mchimento-at-uab.edu Name: Melissa Chimento } } Organization: University of Alabama at Birmingham } } Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM? } } Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They } introduce the NDs before polymerization at room temperature. They said that it forms a “brick” of } polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with } NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any } suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in } the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if } they could check dispersion of the ND in the polymer with it?? } } } Thanks! } } } Melissa Chimento } } University of Alabama at Birmingham-HRIF } } Department of Vision Science } } 205-934-1926 } } } Login Host: 138.26.156.111 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 16, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 11 21:12:43 2012 } 16, 25 -- Received: from znl.com ([206.69.208.20]) } 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0C3ChXI003355 } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:43 -0600 } 16, 25 -- Received: from localhost (localhost [127.0.0.1]) } 16, 25 -- by znl.com (Postfix) with ESMTP id 26484472E6C } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:43 -0600 (CST) } 16, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 16, 25 -- Received: from znl.com ([127.0.0.1]) } 16, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 16, 25 -- with ESMTP id qt2UV-lpHnqw for {microscopy-at-microscopy.com} ; } 16, 25 -- Wed, 11 Jan 2012 21:12:42 -0600 (CST) } 16, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 16, 25 -- by znl.com (Postfix) with ESMTPA id 6D46A472E61 } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:42 -0600 (CST) } 16, 25 -- Message-ID: {4F0E4FAA.8090401-at-microscopy.com} } 16, 25 -- Date: Wed, 11 Jan 2012 21:12:42 -0600 } 16, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 16, 25 -- Reply-To: mchimento-at-uab.edu } 16, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 16, 25 -- MIME-Version: 1.0 } 16, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 16, 25 -- Subject: viaWWW:Nano diamonds dispersed in a polymer TEM?SEM? } 16, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 16, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } } -- } This email was Anti Virus checked by Astaro Security Gateway. http://www.astaro.com
-------------------------------------------------- Ingo Lieberwirth Max Planck Institute for Polymer Research Ackermannweg 10 D-55128 Mainz
Apologies if you receive this more than once as it is going to multiple lists.
Over the last ~18 months, I have been setting up a new electron microprobe lab at a 2800-student primarily undergraduate university in West Virginia. Being curious if there might be any other undergraduate schools with a fully-functioning probe lab, I prepared a compilation of North American probe labs. Through this exercise, I think I have found one other undergraduate probe lab.
Wanting to share, I have posted the compilation on our lab website at: http://academics.concord.edu/microanalysis/OtherLabs.html
The compilation includes a Google Maps view as well as tables listing the labs by institution name and by instrument type. Both academic and government/industry labs are included. Also included are links to the lab web sites where I have them.
Perhaps some of you may find this recent compilation to be useful.
I also could use your help in checking the accuracy and completeness of the compilation. Please take a look and suggest additions/changes as needed. I am sure that I have missed a few.
Thanks and best regards,
- Steve Kuehn
--
---------------- Dr. Stephen C. Kuehn Research Assistant Professor Manager, Electron Microprobe Facility & Tephra Lab Science building, Room 106
Concord University 1000 Vermillion St PO Box 1000, Campus Box F20 Athens, WV 24712-1000
Dear Melissa, I agree with Ingo, my first try would cryo-UM depending on the polymer matrix. The only problem is that the diamond particles are going to fall out of the material and leave nice big gouges in the sections. Another possible solution would be a cryo-fracture and hi-res SEM of the material. You may have to thin the brick down to a manageable thickness in order to fracture it. Good luck, Vicky
Victoria M. Bryg Research Associate - NCSER/USRA NASA Glenn Research Center 216-433-9628
-----Original Message----- } From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, January 11, 2012 10:20 PM To: Bryg, Victoria M. (GRC-REC0)[National Center for Space Exploration Research]
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mchimento-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?
Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They introduce the NDs before polymerization at room temperature. They said that it forms a “brick” of polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if they could check dispersion of the ND in the polymer with it??
Recently, we have noticed more reports of using Durcupan ACM resin in the literature, so we ordered a Durcupan ACM resin kit to try out. I noticed this resin is very viscous, much more so than the Embed-812 resin that we typically use. Does anyone have any particular resin infiltration schedules that have worked well for them for cell cultures and plant and animal tissues? I read that Durcupan causes less shrinkage than other resins, but does it have any other advantages over other resins for certain applications?
Thanks, Shannon Bio-Imaging Center Delaware Biotechnology Institute Newark, DE
==============================Original Headers============================== 2, 25 -- From modla-at-dbi.udel.edu Thu Jan 12 08:34:36 2012 2, 25 -- Received: from newmail.dbi.udel.edu (newmail.dbi.udel.edu [128.175.253.7]) 2, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0CEYaD0025039 2, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Jan 2012 08:34:36 -0600 2, 25 -- Received: from localhost (localhost [127.0.0.1]) 2, 25 -- by newmail.dbi.udel.edu (Postfix) with ESMTP id 5F12014DE003 2, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Jan 2012 09:34:36 -0500 (EST) 2, 25 -- X-Virus-Scanned: amavisd-new at newmail.dbi.udel.edu 2, 25 -- Received: from newmail.dbi.udel.edu ([127.0.0.1]) 2, 25 -- by localhost (newmail.dbi.udel.edu [127.0.0.1]) (amavisd-new, port 10024) 2, 25 -- with ESMTP id uviknHcS53tQ for {Microscopy-at-microscopy.com} ; 2, 25 -- Thu, 12 Jan 2012 09:34:35 -0500 (EST) 2, 25 -- Received: from newmail.dbi.udel.edu (newmail.dbi.udel.edu [128.175.253.7]) 2, 25 -- by newmail.dbi.udel.edu (Postfix) with ESMTP id DCFEA14DE002 2, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Jan 2012 09:34:35 -0500 (EST) 2, 25 -- Date: Thu, 12 Jan 2012 09:34:35 -0500 (EST) 2, 25 -- From: Shannon Modla {modla-at-dbi.udel.edu} 2, 25 -- To: Microscopy-at-microscopy.com 2, 25 -- Subject: Durcupan resin 2, 25 -- Message-ID: {fc47a94f-b7f7-482b-990b-e293247947b5-at-newmail} 2, 25 -- Content-Type: text/plain; charset=utf-8 2, 25 -- Content-Transfer-Encoding: 7bit 2, 25 -- MIME-Version: 1.0 2, 25 -- X-Originating-IP: [128.175.253.84] 2, 25 -- X-Mailer: Zimbra 7.1.1_GA_3196 (ZimbraWebClient - SAF3 (Win)/7.1.1_GA_3196) ==============================End of - Headers==============================
To fracture the sample, deep it in liquid nitrogen, until le nitrogen stops to boil. Then, take it out and put in on an amboss, and hit it with a hammer. To avoid the flakes to fly everywhere, you can put some weeping paper on the amboss that you fold to cover the sample before you hit it. You must do all these steps fast, to limit the reheating of the sample. It's not a very academic methode, nor it is very reproductible, but it works. You can help the fracturing at a wanted place by making a notch with a rasor blade. The way it will break depends of the type of the polymer.
You choose in the flakes some flat pieces for the observation and coat them with carbon. If the ND are really nano, you will need low energy and high resolution. If the "nano" is in the 100 nm range or more, BSE detector, higher energy (and maybe ESEM mode) should work.
Hope it helps
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Le 12/01/2012 04:22, microscopylistserver-noreply-at-microscopy.com a écrit : } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } mchimento-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mchimento-at-uab.edu Name: Melissa Chimento } } Organization: University of Alabama at Birmingham } } Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM? } } Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They } introduce the NDs before polymerization at room temperature. They said that it forms a “brick” of } polymer. IÂ’m not sure what to do with the sample. IÂ’ve had people bring in electro spun fibers with } NDs but not a solid chunk. From what IÂ’ve read polymers are extremely difficult to thin section. Any } suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEMÂ’s in } the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. IÂ’m not sure if } they could check dispersion of the ND in the polymer with it?? } } } Thanks! } } } Melissa Chimento } } University of Alabama at Birmingham-HRIF } } Department of Vision Science } } 205-934-1926 } } } Login Host: 138.26.156.111 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 16, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 11 21:12:43 2012 } 16, 25 -- Received: from znl.com ([206.69.208.20]) } 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0C3ChXI003355 } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:43 -0600 } 16, 25 -- Received: from localhost (localhost [127.0.0.1]) } 16, 25 -- by znl.com (Postfix) with ESMTP id 26484472E6C } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:43 -0600 (CST) } 16, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 16, 25 -- Received: from znl.com ([127.0.0.1]) } 16, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 16, 25 -- with ESMTP id qt2UV-lpHnqw for {microscopy-at-microscopy.com} ; } 16, 25 -- Wed, 11 Jan 2012 21:12:42 -0600 (CST) } 16, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 16, 25 -- by znl.com (Postfix) with ESMTPA id 6D46A472E61 } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:42 -0600 (CST) } 16, 25 -- Message-ID: {4F0E4FAA.8090401-at-microscopy.com} } 16, 25 -- Date: Wed, 11 Jan 2012 21:12:42 -0600 } 16, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 16, 25 -- Reply-To: mchimento-at-uab.edu } 16, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 16, 25 -- MIME-Version: 1.0 } 16, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 16, 25 -- Subject: viaWWW:Nano diamonds dispersed in a polymer TEM?SEM? } 16, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 16, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 32 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Jan 12 10:34:38 2012 9, 32 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154]) 9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0CGYb0r015038 9, 32 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2012 10:34:38 -0600 9, 32 -- Received: from ipcms-smtp.u-strasbg.fr (ipcms-smtp.u-strasbg.fr [130.79.210.9]) 9, 32 -- by mailhost.u-strasbg.fr (8.14.3/jtpda-5.5pre1) with ESMTP id q0CGYaWK032732 9, 32 -- ; Thu, 12 Jan 2012 17:34:36 +0100 (CET) (envelope-from jacques.faerber-at-ipcms.u-strasbg.fr) 9, 32 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 32 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTP id DC9444FAD64; 9, 32 -- Thu, 12 Jan 2012 17:38:34 +0100 (CET) 9, 32 -- X-Virus-Scanned: Debian amavisd-new at ipcms.unistra.fr 9, 32 -- Received: from ipcms-smtp.u-strasbg.fr ([127.0.0.1]) 9, 32 -- by localhost (ipcms-smtp.u-strasbg.fr [127.0.0.1]) (amavisd-new, port 10024) 9, 32 -- with ESMTP id TJUu66jYIsvw; Thu, 12 Jan 2012 17:38:33 +0100 (CET) 9, 32 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 9, 32 -- by ipcms-smtp.u-strasbg.fr (Postfix) with ESMTPSA id C3F664FAD4A; 9, 32 -- Thu, 12 Jan 2012 17:38:33 +0100 (CET) 9, 32 -- Message-ID: {4F0F0BA4.2080602-at-ipcms.u-strasbg.fr} 9, 32 -- Date: Thu, 12 Jan 2012 17:34:44 +0100 9, 32 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 9, 32 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.24) Gecko/20111108 Thunderbird/3.1.16 9, 32 -- MIME-Version: 1.0 9, 32 -- To: mchimento-at-uab.edu, microscopy-at-microscopy.com 9, 32 -- Subject: Re: [Microscopy] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM? 9, 32 -- References: {201201120322.q0C3MR5A028580-at-ns.microscopy.com} 9, 32 -- In-Reply-To: {201201120322.q0C3MR5A028580-at-ns.microscopy.com} 9, 32 -- Content-Type: text/plain; charset=windows-1252; format=flowed 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.2.5 (mailhost.u-strasbg.fr [130.79.200.154]); Thu, 12 Jan 2012 17:34:36 +0100 (CET) 9, 32 -- X-Spam-Status: No, score=-101.2 required=5.0 tests=RP_MATCHES_RCVD, 9, 32 -- USER_IN_WHITELIST autolearn=disabled version=3.3.1 9, 32 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr4.u-strasbg.fr ==============================End of - Headers==============================
Perhaps instead of EM you might be better off investigating via scattering, such as SAXS? That way you could look at the bulk sample and see an average particle spacing, if the technique would permit it (depends on density/spacing of NDs in the polymer).
Alternatively, instead of positive evidence (seeing the NDs) is your work flexible enough to allow for the use of negative evidence instead of positive evidence? In other words, if the NDs all get popped out during microtoming you end up with a system full of voids. Investigation of void spacing could indirectly give you info on ND spacing. This would be dependent on how cleanly the NDs come out of the polymer - if they tear or gouge the sample badly, then this would be a failed method.
-John Papalia
********************** John M. Papalia, Ph.D. Materials Scientist & Job seeker **********************
On 1/12/2012 9:35 AM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Melissa, } I agree with Ingo, my first try would cryo-UM depending on the polymer matrix. The only problem is } that the diamond particles are going to fall out of the material and leave nice big gouges in the } sections. Another possible solution would be a cryo-fracture and hi-res SEM of the material. You may } have to thin the brick down to a manageable thickness in order to fracture it. Good luck, } Vicky } } Victoria M. Bryg } Research Associate - NCSER/USRA } NASA Glenn Research Center } 216-433-9628 } } } } -----Original Message----- } } From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Wednesday, January 11, 2012 10:20 PM } To: Bryg, Victoria M. (GRC-REC0)[National Center for Space Exploration Research] } Subject: [Microscopy] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } mchimento-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mchimento-at-uab.edu Name: Melissa Chimento } } Organization: University of Alabama at Birmingham } } Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM? } } Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They } introduce the NDs before polymerization at room temperature. They said that it forms a “brick” of } polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with } NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any } suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in } the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if } they could check dispersion of the ND in the polymer with it?? } } } Thanks! } } } Melissa Chimento } } University of Alabama at Birmingham-HRIF } } Department of Vision Science } } 205-934-1926 } } } Login Host: 138.26.156.111 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 16, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 11 21:12:43 2012 } 16, 25 -- Received: from znl.com ([206.69.208.20]) } 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0C3ChXI003355 } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:43 -0600 } 16, 25 -- Received: from localhost (localhost [127.0.0.1]) } 16, 25 -- by znl.com (Postfix) with ESMTP id 26484472E6C } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:43 -0600 (CST) } 16, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 16, 25 -- Received: from znl.com ([127.0.0.1]) } 16, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 16, 25 -- with ESMTP id qt2UV-lpHnqw for {microscopy-at-microscopy.com} ; } 16, 25 -- Wed, 11 Jan 2012 21:12:42 -0600 (CST) } 16, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 16, 25 -- by znl.com (Postfix) with ESMTPA id 6D46A472E61 } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:42 -0600 (CST) } 16, 25 -- Message-ID: {4F0E4FAA.8090401-at-microscopy.com} } 16, 25 -- Date: Wed, 11 Jan 2012 21:12:42 -0600 } 16, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 16, 25 -- Reply-To: mchimento-at-uab.edu } 16, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) } Gecko/20100722 Eudora/3.0.4 } 16, 25 -- MIME-Version: 1.0 } 16, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 16, 25 -- Subject: [Filtered] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM? } 16, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 16, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 28, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 12 08:28:09 2012 } 28, 28 -- Received: from znl.com ([206.69.208.20]) } 28, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0CES946011236 } 28, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2012 08:28:09 -0600 } 28, 28 -- Received: from localhost (localhost [127.0.0.1]) } 28, 28 -- by znl.com (Postfix) with ESMTP id 8542A4734F9 } 28, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2012 08:28:09 -0600 (CST) } 28, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 28, 28 -- Received: from znl.com ([127.0.0.1]) } 28, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 28, 28 -- with ESMTP id 51+wMW59PGBS for {microscopy-at-microscopy.com} ; } 28, 28 -- Thu, 12 Jan 2012 08:28:05 -0600 (CST) } 28, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 28, 28 -- by znl.com (Postfix) with ESMTPA id B654A4734EE } 28, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2012 08:28:05 -0600 (CST) } 28, 28 -- Message-ID: {4F0EEDF5.3010907-at-microscopy.com} } 28, 28 -- Date: Thu, 12 Jan 2012 08:28:05 -0600 } 28, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 28, 28 -- Reply-To: "Bryg, Victoria M. (GRC-REC0)[National Center for Space Exploration Research]" {victoria.m.bryg-at-nasa.gov} } 28, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 28, 28 -- MIME-Version: 1.0 } 28, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 28, 28 -- Subject: [Filtered] RE: [Microscopy] viaWWW:Nano diamonds dispersed in a polymer } 28, 28 -- TEM?SEM? } 28, 28 -- References: {201201120320.q0C3KJbp023240-at-ns.microscopy.com} } 28, 28 -- In-Reply-To: {201201120320.q0C3KJbp023240-at-ns.microscopy.com} } 28, 28 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 28, 28 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } -- }
==============================Original Headers============================== 6, 22 -- From jpapalia-at-papalia.net Thu Jan 12 10:38:53 2012 6, 22 -- Received: from vms173005pub.verizon.net (vms173005pub.verizon.net [206.46.173.5]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0CGcrH6021648 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2012 10:38:53 -0600 6, 22 -- MIME-version: 1.0 6, 22 -- Content-transfer-encoding: 8BIT 6, 22 -- Content-type: text/plain; charset=windows-1252; format=flowed 6, 22 -- Received: from [192.168.200.104] ([unknown] [108.46.28.25]) 6, 22 -- by vms173005.mailsrvcs.net 6, 22 -- (Sun Java(tm) System Messaging Server 7u2-7.02 32bit (built Apr 16 2009)) 6, 22 -- with ESMTPA id {0LXP001LV28HFR10-at-vms173005.mailsrvcs.net} for 6, 22 -- microscopy-at-microscopy.com; Thu, 12 Jan 2012 10:38:48 -0600 (CST) 6, 22 -- Message-id: {4F0F0C8E.6090706-at-papalia.net} 6, 22 -- Date: Thu, 12 Jan 2012 11:38:38 -0500 6, 22 -- From: John Papalia {jpapalia-at-papalia.net} 6, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:9.0) Gecko/20111222 6, 22 -- Thunderbird/9.0.1 6, 22 -- To: microscopy-at-microscopy.com, mchimento-at-uab.edu 6, 22 -- Subject: Re: [Microscopy] [Filtered] RE: viaWWW:Nano diamonds dispersed in a 6, 22 -- polymer 6, 22 -- References: {201201121435.q0CEZnjC029680-at-ns.microscopy.com} 6, 22 -- In-reply-to: {201201121435.q0CEZnjC029680-at-ns.microscopy.com} ==============================End of - Headers==============================
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, January 11, 2012 8:22 PM To: John Mardinly
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mchimento-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?
Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They introduce the NDs before polymerization at room temperature. They said that it forms a Â"brickÂ" of polymer. IÂ'm not sure what to do with the sample. IÂ've had people bring in electro spun fibers with NDs but not a solid chunk. From what IÂ've read polymers are extremely difficult to thin section. Any suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEMÂ's in the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. IÂ'm not sure if they could check dispersion of the ND in the polymer with it??
What size are the nanodiamonds are expected to be? If they in the 2 to 5 nm range, and the loading fraction is not too high (~10% or lower by volume), microtoming should work just fine (cryo if Tg { RT). At a 70 or 100 nm slice thickness, the vast majority should be well encapsulated with polymer, and not drop out. A diamond microtome knife shouldn't get hurt either, unless the NDs are a bit bigger, i.e., } 10 nm. We do this routinely for some naturally-occurring polymer-nanodiamond samples, and haven't had to change the diamond knife at all.
For } 10 nm NDs, I would go a with the FIB, as John said, either for direct-slice and view in the FIB if you can get enough contrast, or for TEM imaging if contrast is a problem. For the smaller NDs the surface damage and re-dep may make it hard to get the contrast you need for either FIB-based or TEM-based imaging, unless you have a really good FIB and FIB operator.
Good luck.
Rhonda
On 1/11/2012 10:13 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } mchimento-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mchimento-at-uab.edu Name: Melissa Chimento } } Organization: University of Alabama at Birmingham } } Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM? } } Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They } introduce the NDs before polymerization at room temperature. They said that it forms a “brick” of } polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with } NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any } suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in } the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if } they could check dispersion of the ND in the polymer with it?? } } } Thanks! } } } Melissa Chimento } } University of Alabama at Birmingham-HRIF } } Department of Vision Science } } 205-934-1926 } } } Login Host: 138.26.156.111 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 16, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jan 11 21:12:43 2012 } 16, 25 -- Received: from znl.com ([206.69.208.20]) } 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0C3ChXI003355 } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:43 -0600 } 16, 25 -- Received: from localhost (localhost [127.0.0.1]) } 16, 25 -- by znl.com (Postfix) with ESMTP id 26484472E6C } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:43 -0600 (CST) } 16, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 16, 25 -- Received: from znl.com ([127.0.0.1]) } 16, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 16, 25 -- with ESMTP id qt2UV-lpHnqw for {microscopy-at-microscopy.com} ; } 16, 25 -- Wed, 11 Jan 2012 21:12:42 -0600 (CST) } 16, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 16, 25 -- by znl.com (Postfix) with ESMTPA id 6D46A472E61 } 16, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2012 21:12:42 -0600 (CST) } 16, 25 -- Message-ID: {4F0E4FAA.8090401-at-microscopy.com} } 16, 25 -- Date: Wed, 11 Jan 2012 21:12:42 -0600 } 16, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 16, 25 -- Reply-To: mchimento-at-uab.edu } 16, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 16, 25 -- MIME-Version: 1.0 } 16, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 16, 25 -- Subject: [Filtered] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM? } 16, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 16, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } }
As a reality-sanity check, before preparing the polymer/diamond sample, I would suggest trying to image a suspension of the nano-diamonds on a carbon film support. This is just to be certain of the size of the nano-diamonds. Their size will influence how sliceable they may be with a diamond knife. If they are very small, really nano sized, they may stay put in the polymer and make a nice sample. The larger they are the higher the probability of pull out and knife damage. It would also be good to ask what the researchers think the concentration of nano-diamond is in the polymer.
Years ago I had many researchers ask me to image what they thought were 5-10 nm diameter (lab-made) nano-particles, these were often 100-700 nm diameter particles. This does not happen much anymore but it is sometimes good to check before investing hours of your time. Also nano diamonds in polymer may be easier to image with STEM dark field imaging rather than TEM--if you have the option.
They may want to try cryo-FIB of a block of their sample. It might be possible to get that done by JEOL, FEI --not sure if Hitachi has a cryo FIB. IF the manufacturer can show off the results for marketing purposes they will sometimes do exploratory samples for free.
Good luck, Roseann
Roseann Csencsits, PhD Scientist in Charge, Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
On Jan 11, 2012, at 7:21 PM, microscopylistserver-noreply-at-microscopy.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } mchimento-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mchimento-at-uab.edu Name: Melissa Chimento } } Organization: University of Alabama at Birmingham } } Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM? } } Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They } introduce the NDs before polymerization at room temperature. They said that it forms a “brick” of } polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with } NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any } suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in } the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if } they could check dispersion of the ND in the polymer with it?? } } } Thanks! } } } Melissa Chimento } } University of Alabama at Birmingham-HRIF } } Department of Vision Science } } 205-934-1926 } } } 16, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 16, 25 -- MIME-Version: 1.0 } 16, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 16, 25 -- Subject: [Filtered] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM? } 16, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 16, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 21, 27 -- From microscopylistserver-noreply-at-microscopy.com Fri Jan 13 08:25:46 2012 21, 27 -- Received: from znl.com ([206.69.208.20]) 21, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0DEPkBj026249 21, 27 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2012 08:25:46 -0600 21, 27 -- Received: from localhost (localhost [127.0.0.1]) 21, 27 -- by znl.com (Postfix) with ESMTP id 7834A474255 21, 27 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2012 08:25:46 -0600 (CST) 21, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 21, 27 -- Received: from znl.com ([127.0.0.1]) 21, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 21, 27 -- with ESMTP id NzCz3IMAlFTi for {microscopy-at-microscopy.com} ; 21, 27 -- Fri, 13 Jan 2012 08:25:41 -0600 (CST) 21, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 21, 27 -- by znl.com (Postfix) with ESMTPA id 6E94347424A 21, 27 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2012 08:25:41 -0600 (CST) 21, 27 -- Message-ID: {4F103EE5.6010709-at-microscopy.com} 21, 27 -- Date: Fri, 13 Jan 2012 08:25:41 -0600 21, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 21, 27 -- Reply-To: Roseann Csencsits {rcsencsits-at-lbl.gov} 21, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 21, 27 -- MIME-Version: 1.0 21, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 21, 27 -- Subject: [Filtered] Re: Nano diamonds dispersed in a polymer TEM?SEM? 21, 27 -- References: {201201120321.q0C3LRKB025658-at-ns.microscopy.com} 21, 27 -- In-Reply-To: {201201120321.q0C3LRKB025658-at-ns.microscopy.com} 21, 27 -- Content-Type: text/plain; charset=UTF-8; format=flowed 21, 27 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I greatly appreciate all of the feedback I received to this post. Thank you,
Melissa F. Chimento University of Alabama at Birmingham HRIF SHEL Room 912 1825 University Boulevard Birmingham, AL 35294
LAB #: (205) 934-1926 OFFICE #: (205) 996-6469
mchimento-at-uab.edu
-----Original Message----- } From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, January 11, 2012 9:20 PM To: Melissa Foley Chimento
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mchimento-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?
Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They introduce the NDs before polymerization at room temperature. They said that it forms a “brick” of polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if they could check dispersion of the ND in the polymer with it??
Any advice for EBSD of zircaloys? I tried multi-hour colloidal silica (no patterns, 15-30 kV) and then tried a few minutes of colloidal silica+ammonium hydroxide + hydrogen peroxide (still no patterns, 15-30 kV).
Has anyone had good luck? My next resort is our Gatan Ilion ion polisher, but I'm hoping for a simple answer to let me use my already mounted and polished specimens.
Thanks, Chad Parish
==============================Original Headers============================== 5, 27 -- From parishcm-at-ornl.gov Fri Jan 13 10:49:42 2012 5, 27 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.14.62]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0DGngJU014839 5, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Jan 2012 10:49:42 -0600 5, 27 -- X-SG: RELAYLIST 5, 27 -- X-IronPort-AV: E=Sophos;i="4.71,504,1320642000"; 5, 27 -- d="scan'208";a="4195715" 5, 27 -- Received: from ironport-7603-src.ens.ornl.gov (HELO exchhub2.ornl.gov) ([10.1.86.100]) 5, 27 -- by iron2.ornl.gov with ESMTP/TLS/RC4-MD5; 13 Jan 2012 11:49:42 -0500 5, 27 -- Received: from EXCHMBB.ornl.gov ([192.168.3.201]) by exchhub2.ornl.gov 5, 27 -- ([160.91.2.112]) with mapi; Fri, 13 Jan 2012 11:49:42 -0500 5, 27 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 5, 27 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 5, 27 -- Date: Fri, 13 Jan 2012 11:49:41 -0500 5, 27 -- Subject: EBSD: prepping Zircaloy 5, 27 -- Thread-Topic: EBSD: prepping Zircaloy 5, 27 -- Thread-Index: AczSE1PFco+YWTiFSaGREV61LkUWUg== 5, 27 -- Message-ID: {3C47B404D340EE48A469ADAE2ADBF9665AFB88F034-at-EXCHMBB.ornl.gov} 5, 27 -- Accept-Language: en-US 5, 27 -- Content-Language: en-US 5, 27 -- X-MS-Has-Attach: 5, 27 -- X-MS-TNEF-Correlator: 5, 27 -- acceptlanguage: en-US 5, 27 -- Content-Type: text/plain; charset="us-ascii" 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Transfer-Encoding: 8bit 5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0DGngJU014839 ==============================End of - Headers==============================
Dear All, maybe somebody out there can help me with classifying this diatomee: http://www.electronmicroscopy.info/diatomee/index.htm
I found it on a ca 300 year old piece of slag from iron smelting in a valley in the northern part of Bavaria, Germany. The small river I found it in is not too warm during the year, height above MSL is ca. 800 to 400 meter. Google earth link is at http://www.electronmicroscopy.info/diatomee/iron_slug.kmz
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
That a great way to start a work week: A diatom question! Unfortunately I don't have any of my diatom references at work, but I have a question of my own.
I cutting cryo-thin sections of silica loaded silicone rubber with a diamond histology knife (it's all I have). It as a Tg of -127C. I'm working dry at -140C using sugar water to pick-up the thin sections for TEM imaging at 80kv. I don't know the loading of silica in the silicone polymer. The microtome is a RMC microtome with cryo-stage.
It's not going well. I can't seem to get to 100 nm. The color guide, based on the interference of light, suggests I at 280nm. In the few thin spots or tears I can see the particles of silica I'm after but the rest of the section is too thick.
Has anyone practical experience with cryo microtoming of silica loaded silicone? I'm open to ideas
Thanks.... Frank
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 15, 25 -- From frank_karl-at-ardl.com Mon Jan 16 07:57:54 2012 15, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0GDvsO1005668 15, 25 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2012 07:57:54 -0600 15, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 15, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Mon, 16 15, 25 -- Jan 2012 08:57:53 -0500 15, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 15, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 15, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 15, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 15, 25 -- Date: Mon, 16 Jan 2012 08:57:52 -0500 15, 25 -- Subject: microtoming silicone rubber for TEM 15, 25 -- Thread-Topic: microtoming silicone rubber for TEM 15, 25 -- Thread-Index: AczUVta0sWJNX7yfTOGKfPQ4yJ1cNg== 15, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF1507701584541F3-at-exchange2k7.ad.ardl.com} 15, 25 -- Accept-Language: en-US 15, 25 -- Content-Language: en-US 15, 25 -- X-MS-Has-Attach: 15, 25 -- X-MS-TNEF-Correlator: 15, 25 -- acceptlanguage: en-US 15, 25 -- Content-Type: text/plain; charset="iso-8859-1" 15, 25 -- MIME-Version: 1.0 15, 25 -- Content-Transfer-Encoding: 8bit 15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0GDvsO1005668 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fabrice.noel-at-sydney.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Position Vacant - Postdoctoral Research Associate - In Situ Deformation TEM
Message: Dear all,
The Faculty of Engineering at the University of Sydney is currently seeking a Postdoctoral Research Associate. Visit sydney.edu.au/positions and search by the reference number for more information and to apply.
POSTDOCTORAL RESEARCH ASSOCIATE - IN-SITU DEFORMATION TEM SCHOOL OF AEROSPACE, MECHANICAL AND MECHATRONIC ENGINEERING REFERENCE NO. 1995/1111
• Well established faculty • Cutting-edge research • Work with internationally renowned academics
We currently seek a Postdoctoral Research Associate to work on an ARC funded project. This project aims to apply state-of-the-art in-situ deformation transmission electron microscopy techniques to reveal how crystalline defects in nanostructured metals and alloys interact with each other and to link directly the interactions with the mechanical behaviour of the materials. The results will enable structural design of advanced metallic materials with optimum mechanical properties.
This is an opportunity to work closely with Associate Professor Xiaozhou Liao and conduct research at the Centre for Advanced Materials Technology (CAMT) within the school. You will be part of a school which has a high international profile for its quality research over a wide field in materials characterisation and processing, nanotechnology, advanced manufacturing, solid mechanics and biotechnology.
Having completed a PhD in Material Science (or relevant area), you will have extensive experience in transmission electron microscopy (TEM) with a solid background and understanding in the structure and mechanical properties of materials. CLOSING DATE: 14 February 2012 (11:30pm Sydney time) or until a successful individual has been identified.
Stefan, I pulled out my only reference to European diatoms, Heurch's Treatise on Diatomaceae published in 1896. I just have a photographic copy of it so the images aren't that sharp and the line drawings are so-so but I think your diatom best matches Navicula viridula.
hope this helps ..... Frank
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: Monday, January 16, 2012 7:00 AM To: Frank Karl
Dear All, maybe somebody out there can help me with classifying this diatomee: http://www.electronmicroscopy.info/diatomee/index.htm
I found it on a ca 300 year old piece of slag from iron smelting in a valley in the northern part of Bavaria, Germany. The small river I found it in is not too warm during the year, height above MSL is ca. 800 to 400 meter. Google earth link is at http://www.electronmicroscopy.info/diatomee/iron_slug.kmz
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
==============================Original Headers============================== 7, 20 -- From stefan.diller-at-t-online.de Mon Jan 16 05:47:05 2012 7, 20 -- Received: from mailout10.t-online.de (mailout10.t-online.de [194.25.134.21]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0GBl54R018373 7, 20 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2012 05:47:05 -0600 7, 20 -- Received: from fwd06.aul.t-online.de (fwd06.aul.t-online.de ) 7, 20 -- by mailout10.t-online.de with smtp 7, 20 -- id 1Rml1j-0005cT-HB; Mon, 16 Jan 2012 12:47:03 +0100 7, 20 -- Received: from mac-pro.speedport.ip (Ek0GG4ZvYhvW3NlOchBGc4MTb9Ab6bQhsmHpIxjlRPtv5NUEO3okUtR5N2zlxTawqD-at-[93.222.91.66]) by fwd06.t-online.de 7, 20 -- with esmtp id 1Rmkr7-1uBSEa0; Mon, 16 Jan 2012 12:36:05 +0100 7, 20 -- Message-ID: {4F140BA5.7070708-at-t-online.de} 7, 20 -- Date: Mon, 16 Jan 2012 12:36:05 +0100 7, 20 -- From: Stefan Diller {stefan.diller-at-t-online.de} 7, 20 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.6; rv:8.0) Gecko/20111105 Thunderbird/8.0 7, 20 -- MIME-Version: 1.0 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- Subject: Classifying diatomee 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit 7, 20 -- X-ID: Ek0GG4ZvYhvW3NlOchBGc4MTb9Ab6bQhsmHpIxjlRPtv5NUEO3okUtR5N2zlxTawqD 7, 20 -- X-TOI-MSGID: 9bf37b38-4527-422c-b103-4ef4ba16238f ==============================End of - Headers==============================
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 18, 28 -- From frank_karl-at-ardl.com Tue Jan 17 07:39:05 2012 18, 28 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0HDd4l2031796 18, 28 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2012 07:39:05 -0600 18, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 18, 28 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Tue, 17 18, 28 -- Jan 2012 08:39:04 -0500 18, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 18, 28 -- To: "'stefan.diller-at-t-online.de'" {stefan.diller-at-t-online.de} , 18, 28 -- "Microscopy 18, 28 -- Listserver (E-mail)" {microscopy-at-microscopy.com} 18, 28 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 18, 28 -- Return-Receipt-To: {frank_karl-at-ardl.com} 18, 28 -- Date: Tue, 17 Jan 2012 08:39:04 -0500 18, 28 -- Subject: RE: [Microscopy] Classifying diatomee 18, 28 -- Thread-Topic: [Microscopy] Classifying diatomee 18, 28 -- Thread-Index: AczURlMZ+V7XgXuzR+Wx2/cE1so5UAA1cjKg 18, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF1507701584541F5-at-exchange2k7.ad.ardl.com} 18, 28 -- In-Reply-To: {201201161159.q0GBxVem001576-at-ns.microscopy.com} 18, 28 -- Accept-Language: en-US 18, 28 -- Content-Language: en-US 18, 28 -- X-MS-Has-Attach: 18, 28 -- X-MS-TNEF-Correlator: 18, 28 -- acceptlanguage: en-US 18, 28 -- Content-Type: text/plain; charset="iso-8859-1" 18, 28 -- MIME-Version: 1.0 18, 28 -- Content-Transfer-Encoding: 8bit 18, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0HDd4l2031796 ==============================End of - Headers==============================
Dear Listers Looking at a mucosal animal epithelium with SEM, I came across a creature that is strange to me. It's like 30 microns long tube at the shape of L, the short arm has a hole at the end and the long one is twisted like a screw. Maybe it is a short of borrelia? Whoever is familiar with microorganisms, please have a look at
Thanks all for your help concerning my diatomee problem.
The most probable outcome is "Hippodonta capitata", as suggested by Prof. Hans Rudolf Thierstein. He also pointed me to a really impressing source on diatomee: http://westerndiatoms.colorado.edu/taxa/species/hippodonta_capitata
Best wishes, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Maybe this question would be best for Professor Thierstein, but is there a good online guide for classifying diatoms?
I have looked at quite a few diatoms in water samples over the years but have not embarked on trying to classify them. They were simply a significant part of the solids load of the water sample. Many looked like your sample.
However, from a quick search prompted by your question, I see similarities between hippodonta, planothidium, and navicula. The differences appear to be subtle between them. Therefore, I wonder about a good, definitive classification scheme.
Warren Straszheim
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: Tuesday, January 17, 2012 9:38 AM To: wesaia-at-iastate.edu
Thanks all for your help concerning my diatomee problem.
The most probable outcome is "Hippodonta capitata", as suggested by Prof. Hans Rudolf Thierstein. He also pointed me to a really impressing source on diatomee: http://westerndiatoms.colorado.edu/taxa/species/hippodonta_capitata
Best wishes, Stefan
==============================Original Headers============================== 11, 34 -- From wesaia-at-iastate.edu Tue Jan 17 09:55:14 2012 11, 34 -- Received: from mailexch-1.iastate.edu (mailexch-1.iastate.edu [129.186.140.21]) 11, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0HFtEoA021239 11, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2012 09:55:14 -0600 11, 34 -- Received: from ITSEDGE1D.its.iastate.edu (itsedge1d.its.iastate.edu [10.10.82.5]) 11, 34 -- by mailexch-1.iastate.edu (8.14.4/8.12.10) with ESMTP id q0HFtDMS026826 11, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2012 09:55:13 -0600 11, 34 -- Received: from ITSHUB4A.its.iastate.edu (10.10.83.10) by 11, 34 -- ITSEDGE1D.its.iastate.edu (10.10.82.5) with Microsoft SMTP Server (TLS) id 11, 34 -- 14.1.339.1; Tue, 17 Jan 2012 09:55:13 -0600 11, 34 -- Received: from ITSDAG4A.its.iastate.edu ([169.254.4.104]) by 11, 34 -- ITSHUB4A.its.iastate.edu ([10.10.83.10]) with mapi id 14.01.0339.001; Tue, 17 11, 34 -- Jan 2012 09:55:13 -0600 11, 34 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 11, 34 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 34 -- Subject: RE: [Microscopy] Classifying diatomee - solved 11, 34 -- Thread-Topic: [Microscopy] Classifying diatomee - solved 11, 34 -- Thread-Index: AQHM1S37PYFZgNNOXU2Rcbzdj8bIP5YQs+2w 11, 34 -- Date: Tue, 17 Jan 2012 15:55:12 +0000 11, 34 -- Message-ID: {0ED4049522D32A45AD4183B34807322B019155-at-ITSDAG4A.its.iastate.edu} 11, 34 -- References: {201201171537.q0HFbrva004889-at-ns.microscopy.com} 11, 34 -- In-Reply-To: {201201171537.q0HFbrva004889-at-ns.microscopy.com} 11, 34 -- Accept-Language: en-US 11, 34 -- Content-Language: en-US 11, 34 -- X-MS-Has-Attach: 11, 34 -- X-MS-TNEF-Correlator: 11, 34 -- x-originating-ip: [10.64.191.29] 11, 34 -- Content-Type: text/plain; charset="us-ascii" 11, 34 -- MIME-Version: 1.0 11, 34 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2012.1.17.154214 11, 34 -- X-ISUMailhub-test: Gauge=XXIII, Probability=23%, Report=' 11, 34 -- RATWARE_RCVD_BONUS_SPC 2.0, TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1500_1599 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, FROM_NAME_PHRASE 0, RDNS_NXDOMAIN 0, RDNS_SUSP 0, RDNS_SUSP_GENERIC 0, WEBMAIL_SOURCE 0, WEBMAIL_XOIP 0, WEBMAIL_X_IP_HDR 0, __ANY_URI 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOIP 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS ' 11, 34 -- Content-Transfer-Encoding: 8bit 11, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0HFtEoA021239 ==============================End of - Headers==============================
http://books.google.com/ebooks?id=mzYDAAAAQAAJ&dq=diatoms%20of%20united%20states%20subject%3A%22Science%22&lr&as_brr=5&ei=26sVT4eAKIz6M5KvpOoB&source=webstore_bookcard Paleontology by Richard Owen
http://books.google.com/ebooks?id=rZY_AAAAYAAJ&dq=diatoms%20of%20united%20states%20subject%3A%22Science%22&lr&as_brr=5&ei=ickVT-WcJoPUM4e8re0L&source=webstore_bookcard (A Treatise on the Diatomacea)
Cheers,
FCM
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
If one looks long enough, one may actually see something frozen in time that flashes one's mind to a new state. 1. The day that I asked myself how the hydrogen atom's mass could actually be: 1.0008. 2. The day, while looking at a slide of some tissue, I saw a leukocyte that had been caught sneaking in or out of a capillary: http://www.annualreviews.org/doi/abs/10.1146/annurev-pathol-011110-130224
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: Tuesday, January 17, 2012 10:44 AM To: Monson, Frederick
Thanks all for your help concerning my diatomee problem.
The most probable outcome is "Hippodonta capitata", as suggested by Prof. Hans Rudolf Thierstein. He also pointed me to a really impressing source on diatomee: http://westerndiatoms.colorado.edu/taxa/species/hippodonta_capitata
Best wishes, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both frankehrenfeld-at-iatl.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: frankehrenfeld-at-iatl.com Name: Frank Ehrenfeld
Organization: iATL
Title-Subject: [Filtered] Wanted, TEM
Message: Small laboratory in New Jersey is looking to purchase a well maintained used TEM. Ideally weÂ’re looking for a Hitachi Model H-600AB or equivalent. We may also be interested in any ancillary support or sample prep equipment. We can arrange instrument verification, disassembly, packaging, and transportation. If anyone on ListServe knows of any other sources please also contact us at frankehrenfeld-at-iatl.com, ericsnyder-at-iatl.com, or call at 877-428-4285. Thanks.
Hi all, A student wants to use high pressure freezing on dinoflagellates (size approx. 10 microns). Has anyone done this? If yes, did you wick the dinos into cellulose capillary tubes prior to freezing? Or, is there a better method for keeping track of them?
thanks, Beth
==============================Original Headers============================== 3, 20 -- From beth-at-plantbio.uga.edu Wed Jan 18 11:25:30 2012 3, 20 -- Received: from dogwood.plantbio.uga.edu (pbmail.plantbio.uga.edu [128.192.26.3]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0IHPUeA008908 3, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jan 2012 11:25:30 -0600 3, 20 -- X-Footer: cGxhbnRiaW8udWdhLmVkdQ== 3, 20 -- Received: from [128.192.26.69] ([128.192.26.69]) 3, 20 -- (authenticated user beth-at-plantbio.uga.edu) 3, 20 -- by dogwood.plantbio.uga.edu 3, 20 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 3, 20 -- for microscopy-at-microscopy.com; 3, 20 -- Wed, 18 Jan 2012 12:25:20 -0500 3, 20 -- Message-Id: {867FFCC1-4B57-4374-9433-82CCD4971F8E-at-plantbio.uga.edu} 3, 20 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 3, 20 -- To: microscopy microscopy {microscopy-at-microscopy.com} 3, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- Mime-Version: 1.0 (Apple Message framework v936) 3, 20 -- Subject: hpf - dinoflagellates 3, 20 -- Date: Wed, 18 Jan 2012 12:25:20 -0500 3, 20 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Having installed a FEG image analyzer and comparing its results with our tungsten SEM, we are seeing a slight bias with respect to brighter particles measuring smaller (possibly more accurately) with the FEG. The image is backscattered electrons and we use a fixed background threshold for isolating particles from the epoxy. It occurred to me that clipping the edge at the same background value would make brighter particles larger depending on how "fuzzy" the edge is. It would even be possible to predict this "bright versus dark" bias if the SEM's spot size was determined. Some difference should be associated with each SEM's magnification calibration, but I'm accounting for that by first relating calibration to the darkest minerals (quartz which is abundant).
I thought before I re-invent the wheel and get involved with the math and all the other possible variables (e.g., pixel resolution, irregular perimeters, ...) that I'd ask my peers if this has been done before(?) ... leastwise, I haven't yet found a reference ...
TIA & Cheerios from the Avalon ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Michael Shaffer SEM-MLA Research Coordinator Bruneau Centre for Research & Innovation Memorial University St. John's, Newfoundland, Canada Information: http://www.mun.ca/creait/maf/ Scheduling: http://www.mun.ca/creait/maf/SEM-MLA/calendar.php (709) 864-6799 (Ofc) (709) 864-6790 (Lab) cogito ergo ZzoooomM
==============================Original Headers============================== 5, 25 -- From michael-at-shaffer.net Wed Jan 18 13:30:06 2012 5, 25 -- Received: from smtprelay.hostedemail.com (smtprelay0203.hostedemail.com [216.40.44.203]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0IJU69W028041 5, 25 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jan 2012 13:30:06 -0600 5, 25 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254]) 5, 25 -- by smtprelay06.hostedemail.com (Postfix) with SMTP id 7018086623 5, 25 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jan 2012 19:30:06 +0000 (UTC) 5, 25 -- X-Panda: scanned! 5, 25 -- X-Session-Marker: 6D69636861656C40736861666665722E6E6574 5, 25 -- X-Filterd-Recvd-Size: 2205 5, 25 -- Received: from calvin.creait.mun.ca (calvin.creait.mun.ca [134.153.58.105]) 5, 25 -- (Authenticated sender: michael-at-shaffer.net) 5, 25 -- by omf02.hostedemail.com (Postfix) with ESMTP 5, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jan 2012 19:30:06 +0000 (UTC) 5, 25 -- From: michael shaffer {michael-at-shaffer.net} 5, 25 -- Content-Type: text/plain; charset=us-ascii 5, 25 -- Subject: =?windows-1252?Q?SEM=3A_how_much_larger_are_bright_particles_tha?= 5, 25 -- =?windows-1252?Q?n_dark=3F_=85?= 5, 25 -- Date: Wed, 18 Jan 2012 16:00:08 -0330 5, 25 -- Message-Id: {1D70A46B-DBE4-4B2D-B90B-4275B47ADE1A-at-shaffer.net} 5, 25 -- To: MSA Microscopy list {Microscopy-at-microscopy.com} 5, 25 -- Mime-Version: 1.0 (Apple Message framework v1251.1) 5, 25 -- X-Mailer: Apple Mail (2.1251.1) 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0IJU69W028041 ==============================End of - Headers==============================
That doesn't quite make sense to me. I don't see how changing to a higher resolution would lead to what you describe.
In a previous research life, I did a lot of image analysis on minerals in coal. The brighter ones (primarily pyrite) were cut much lower than half max when the threshold was set to detect the darker minerals which comprised the bulk of the mineral matter. Therefore, I expected the pyrite to be measured larger than actual size. The amount of error related to the spot size and the "fuzziness" of the signal. However, the effect was far beyond what I would expect the difference to be between W and field-emission guns.
I would look into other things that might have changed with the changeover.
Is the response of the BSE signal the same, or have they introduced some non-linearity that might have changed how the threshold is set? I would be interested in seeing the relative brightness of the various minerals under similar setups.
Has the software adapted some sort of local thresholding? I know I would have appreciated that back in the day, but we didn't have the computing power to well implement it.
I'd be interested in continuing the conversation off-line if that would be more expedient.
Warren Straszheim former operator of a LeMont Scientific DB-10 image analyzer, circa 1981. ________________________________________ X-from: michael-at-shaffer.net [michael-at-shaffer.net] Sent: Wednesday, January 18, 2012 1:30 PM To: wesaia-at-iastate.edu
Having installed a FEG image analyzer and comparing its results with our tungsten SEM, we are seeing a slight bias with respect to brighter particles measuring smaller (possibly more accurately) with the FEG. The image is backscattered electrons and we use a fixed background threshold for isolating particles from the epoxy. It occurred to me that clipping the edge at the same background value would make brighter particles larger depending on how "fuzzy" the edge is. It would even be possible to predict this "bright versus dark" bias if the SEM's spot size was determined. Some difference should be associated with each SEM's magnification calibration, but I'm accounting for that by first relating calibration to the darkest minerals (quartz which is abundant).
I thought before I re-invent the wheel and get involved with the math and all the other possible variables (e.g., pixel resolution, irregular perimeters, ...) that I'd ask my peers if this has been done before(?) ... leastwise, I haven't yet found a reference ...
TIA & Cheerios from the Avalon ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Michael Shaffer SEM-MLA Research Coordinator Bruneau Centre for Research & Innovation Memorial University St. John's, Newfoundland, Canada Information: http://www.mun.ca/creait/maf/ Scheduling: http://www.mun.ca/creait/maf/SEM-MLA/calendar.php (709) 864-6799 (Ofc) (709) 864-6790 (Lab) cogito ergo ZzoooomM
==============================Original Headers============================== 12, 34 -- From wesaia-at-iastate.edu Wed Jan 18 13:49:22 2012 12, 34 -- Received: from mailexch-1.iastate.edu (mailexch-1.iastate.edu [129.186.140.21]) 12, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0IJnMmd012706 12, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jan 2012 13:49:22 -0600 12, 34 -- Received: from ITSEDGE1D.its.iastate.edu (itsedge1d.its.iastate.edu [10.10.82.5]) 12, 34 -- by mailexch-1.iastate.edu (8.14.4/8.12.10) with ESMTP id q0IJnLrN011554 12, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jan 2012 13:49:21 -0600 12, 34 -- Received: from ITSHUB4A.its.iastate.edu (10.10.83.10) by 12, 34 -- ITSEDGE1D.its.iastate.edu (10.10.82.5) with Microsoft SMTP Server (TLS) id 12, 34 -- 14.1.339.1; Wed, 18 Jan 2012 13:49:21 -0600 12, 34 -- Received: from ITSDAG4A.its.iastate.edu ([169.254.4.104]) by 12, 34 -- ITSHUB4A.its.iastate.edu ([10.10.83.10]) with mapi id 14.01.0339.001; Wed, 18 12, 34 -- Jan 2012 13:49:20 -0600 12, 34 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 12, 34 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 34 -- Subject: RE: [Microscopy] SEM: how much larger are bright particles tha 12, 34 -- Thread-Topic: [Microscopy] SEM: how much larger are bright particles tha 12, 34 -- Thread-Index: AQHM1het6dhErwLJDUe6aDmBoYXXUZYShBHh 12, 34 -- Date: Wed, 18 Jan 2012 19:49:20 +0000 12, 34 -- Message-ID: {0ED4049522D32A45AD4183B34807322B019E43-at-ITSDAG4A.its.iastate.edu} 12, 34 -- References: {201201181930.q0IJUjBD028913-at-ns.microscopy.com} 12, 34 -- In-Reply-To: {201201181930.q0IJUjBD028913-at-ns.microscopy.com} 12, 34 -- Accept-Language: en-US 12, 34 -- Content-Language: en-US 12, 34 -- X-MS-Has-Attach: 12, 34 -- X-MS-TNEF-Correlator: 12, 34 -- x-originating-ip: [129.186.227.19] 12, 34 -- Content-Type: text/plain; charset="us-ascii" 12, 34 -- MIME-Version: 1.0 12, 34 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2012.1.18.193315 12, 34 -- X-ISUMailhub-test: Gauge=XXIII, Probability=23%, Report=' 12, 34 -- RATWARE_RCVD_BONUS_SPC 2.0, TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, FROM_NAME_PHRASE 0, RDNS_NXDOMAIN 0, RDNS_SUSP 0, RDNS_SUSP_GENERIC 0, WEBMAIL_SOURCE 0, WEBMAIL_XOIP 0, WEBMAIL_X_IP_HDR 0, __ANY_URI 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOIP 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS ' 12, 34 -- Content-Transfer-Encoding: 8bit 12, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0IJnMmd012706 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both amy.albin-at-utoledo.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: amy.albin-at-utoledo.edu Name: Amy Albin
Organization: University of Toledo
Title-Subject: [Filtered] LKB 2178 Knifemaker II
Message: I was wondering if anyone knew where to find a manual for a LKB 2178 Knifemaker II? So far, google hasnt been all that helpful. Thanks!
============================================================================================================== Good Morning,
Dear Amy, just to let you know: The whole LKB-product branch has been taken over some 10 years ago by LEICA (cf. http://www.leica-microsystems.com/). So you'll find some results if you search "Google" by the phrase } LEICA 2178 KnifeMaker II { or } LEICA 2178 KnifeMaker II AND Manual {.
Since I have not found the Type you were asking for in my own PC-files (Reichert, LKB, LEICA, ZEISS, etc.) I googled in my own interest and therefore I - exceptionally and hopefully tolerated by the listers - communicate those results I found quickly (and perhaps could be a short way out of your dilemma):
Within the first 20 results there were no results for a {MANUAL available}
but, perhaps you could be supported by EM or Imaging Lab's posessing such a KnifeMaker Type: cf: http://www.memphis.edu/imc/services.htm a Lab which is - unfortunately - some 500 miles away from your location:
Staff communication http://www.memphis.edu/imc/staff.htm : Dr. Omar Skalli Director oskalli-at-memphis.edu Phone: (901) 678-2034 Office: 101 Life Science Bldg
Further information on use of the IMC, including fee schedules, can be obtained by contacting Ms. Lou G. Boykins, Laboratory Coordinator at (901) 678-4233 (phone); (901) 678-4457 (FAX) or lgboykns-at-memphis.edu Ms. Lou Boykins Laboratory Coordinator Phone: (901) 678-2034 Office: 101 Life Science Bldg
Ms. Renada Scott Research Technician II rjscott3-at-memphis.edu Phone: (901) 678-2034 Office: 101 Life Science Bldg Perhaps they can send you a copy of their manual.
At the moment of writing this I do not know about a positive response from any Listserver member.
They are listing: "This instruction manual describes the function and operation of the LKB 2178 KnifeMaker II. LKB 2178 KnifeMaker II Instruction Manual Make: LKB Model: 2178 Reference No: M-050110204 This instruction manual describes the function and operation of the LKB 2178 KnifeMaker II BUT: Disclaimer for all manuals: Manuals listed below are not for copy, sale and/or redistribution. The manuals listed below are used by HiTechTrader.com to repair and maintain equipment. Please contact us (== cf: http://www.hitechtrader.com/contact.cfm?PgID=115 ) if you need any assistance "
Hoping this perhaps was/is of help to you,
best regards, Wolfgang MUSS PhD EM-Lab Univ. Inst. Pathology SALZBURG-AUSTRIA
=======================================
Von: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Gesendet: Donnerstag, 19. Jänner 2012 00:40 An: Muß Wolfgang Betreff: [Microscopy] manual for a LKB 2178 Knifemaker --------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I was wondering if anyone knew where to find a manual for a LKB 2178 Knifemaker II? So far, google hasnt been all that helpful. Thanks!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both amy.albin-at-utoledo.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bplowman-at-pacific.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bplowman-at-pacific.edu Name: Barbara Plowman
Organization: University of the Pacific, Arthur A. Dugoni School of Dentistry
Title-Subject: [Filtered] Uranyl acetate
Message: I have been having a problem with getting uranyl acetate to go into solution. I have been using the same bottle of UA for over 22 years. I have not had any problems when I was using nanopure water, but now that I am using distilled water, I can't seem to make a 3% solution of UAaq. Is there anything I could do to help it go into solution? I am near the end of the bottle. Maybe I should just buy another bottle? Ot try to get the lab to get the nanopure water working again ( I think they need a special filter). Or would adding a little methanol help? I am open to suggestions! Thank you.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both acamacho-at-rd.us.loreal.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Hello dear colleagues, I'm seeking your help because I have two mineral samples with same overall composition (according to my EDS, XRD's are also the same for both) but have different provenance and different performance. I believe the difference is in some trace elements but I need to confirm. I would like to try cathodoluminescence before any exotic technique like neutron activation or PIXE but I don't have a CL detector in house, can you recommend any lab or institution where I could get a couple of samples analyzed? (I apologize for introducing a bit of a commercial here but I haven't been very succesful in finding this info) Thanks in advance for you advice
Good Morning, Let me recommend MicroTrace to you. They are north of Chicago and I believe they can help you out. Here's a link:
http://www.microtracescientific.com/
Good luck........ Frank Karl ARDL
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, January 19, 2012 9:24 PM To: Frank Karl
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both acamacho-at-rd.us.loreal.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Hello dear colleagues, I'm seeking your help because I have two mineral samples with same overall composition (according to my EDS, XRD's are also the same for both) but have different provenance and different performance. I believe the difference is in some trace elements but I need to confirm. I would like to try cathodoluminescence before any exotic technique like neutron activation or PIXE but I don't have a CL detector in house, can you recommend any lab or institution where I could get a couple of samples analyzed? (I apologize for introducing a bit of a commercial here but I haven't been very succesful in finding this info) Thanks in advance for you advice
==============================Original Headers============================== 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Jan 19 20:06:19 2012 8, 25 -- Received: from znl.com ([206.69.208.20]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0K26JJ1004405 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jan 2012 20:06:19 -0600 8, 25 -- Received: from localhost (localhost [127.0.0.1]) 8, 25 -- by znl.com (Postfix) with ESMTP id C078E4791F3 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jan 2012 20:06:19 -0600 (CST) 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 8, 25 -- Received: from znl.com ([127.0.0.1]) 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 8, 25 -- with ESMTP id CGfxcEuf-4rp for {microscopy-at-microscopy.com} ; 8, 25 -- Thu, 19 Jan 2012 20:06:19 -0600 (CST) 8, 25 -- Received: from NestorPwrBk.local (unknown [12.46.250.195]) 8, 25 -- by znl.com (Postfix) with ESMTPA id 335BB4791E8 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jan 2012 20:06:19 -0600 (CST) 8, 25 -- Message-ID: {4F18CC1A.5050007-at-microscopy.com} 8, 25 -- Date: Thu, 19 Jan 2012 21:06:18 -0500 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 8, 25 -- Reply-To: acamacho-at-rd.us.loreal.com 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 8, 25 -- MIME-Version: 1.0 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 8, 25 -- Subject: viaWWW:Cathodoluminescence 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 20, 28 -- From frank_karl-at-ardl.com Fri Jan 20 06:50:53 2012 20, 28 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0KCopVs031582 20, 28 -- for {microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 06:50:51 -0600 20, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 20, 28 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 20 20, 28 -- Jan 2012 07:50:50 -0500 20, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 20, 28 -- To: "'acamacho-at-rd.us.loreal.com'" {acamacho-at-rd.us.loreal.com} , 20, 28 -- "Microscopy 20, 28 -- Listserver (E-mail)" {microscopy-at-microscopy.com} 20, 28 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 20, 28 -- Return-Receipt-To: {frank_karl-at-ardl.com} 20, 28 -- Date: Fri, 20 Jan 2012 07:50:50 -0500 20, 28 -- Subject: RE: [Microscopy] viaWWW:Cathodoluminescence 20, 28 -- Thread-Topic: [Microscopy] viaWWW:Cathodoluminescence 20, 28 -- Thread-Index: AczXGpTwOWUhqebiTeyKROU/R3RO3gAVxcyA 20, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF1507701584541FD-at-exchange2k7.ad.ardl.com} 20, 28 -- In-Reply-To: {201201200223.q0K2NvR6017273-at-ns.microscopy.com} 20, 28 -- Accept-Language: en-US 20, 28 -- Content-Language: en-US 20, 28 -- X-MS-Has-Attach: 20, 28 -- X-MS-TNEF-Correlator: 20, 28 -- acceptlanguage: en-US 20, 28 -- Content-Type: text/plain; charset="iso-8859-1" 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Transfer-Encoding: 8bit 20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0KCopVs031582 ==============================End of - Headers==============================
I had the same problem making a 2% solution about 20 years ago - using old UA. As soon as I started using a new supply the UA went into solution as I thought it should.
If your UA comes in a can it is not a bad idea to store the bottle in the can between uses. I have found that this extends the useable life of the crystals until I empty the bottle.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Message: I have been having a problem with getting uranyl acetate to go into solution. I have been using the same bottle of UA for over 22 years. I have not had any problems when I was using nanopure water, but now that I am using distilled water, I can't seem to make a 3% solution of UAaq. Is there anything I could do to help it go into solution? I am near the end of the bottle. Maybe I should just buy another bottle? Ot try to get the lab to get the nanopure water working again ( I think they need a special filter). Or would adding a little methanol help? I am open to suggestions! Thank you.
Hi, I have no experiencing with aging uranyl acetate. But am curious how time could cause a crystal to become insoluble. Many chemicals on the lab shelf absorb water over time (they often form cakes). This make them difficult to weigh out but I cannot think why it would hinder solubility. Alternatively, could an oxide be forming on the surface of the grains, an oxide might in insoluble (think rust). If the UA powder is not super fine, I guess you could try to grind it with a mortar and pestle to expose fresh surface, although from a safety point of you that seems dubious (UA aerosol -- yumm!). Anyway, I'd be interested to hear if anyone actually knows how time leads to insolubility (of powders -- not mentality, smile).
As ever Tobias
At 9:01 AM -0600 1/20/12, connellyps-at-nhlbi.nih.gov wrote: } } } Barbara, } } I had the same problem making a 2% solution about 20 years ago - } using old UA. As soon as I started using a new supply the UA went } into solution as I thought it should. } } If your UA comes in a can it is not a bad idea to store the bottle } in the can between uses. I have found that this extends the useable } life of the crystals until I empty the bottle. } } Pat } } Patricia Stranen Connelly } Research Assistant } NHLBI Electron Microscopy Core } National Institutes of Health } 14 Service Road West } Bldg. 14E - Rm. 111B MSC 5570 } Bethesda, MD 20892-5570 } Phone 301-496-3491 } FAX 301-402-0170 } connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} } } Opinions and experiences related are those of Pat Connelly and do } not represent the NIH. This message is not confidential and can be } freely shared and reproduced. } } } --------------------------------------------------------------------------- } Email: bplowman-at-pacific.edu Name: Barbara Plowman } Organization: University of the Pacific, Arthur A. Dugoni School of } Dentistry } Title-Subject: [Filtered] Uranyl acetate } } Message: I have been having a problem with getting uranyl acetate to go } into solution. I have been using the same bottle of UA for over 22 } years. I have not had any problems when I was using nanopure water, but } now that I am using distilled water, I can't seem to make a 3% solution } of UAaq. Is there anything I could do to help it go into solution? I am } near the end of the bottle. Maybe I should just buy another bottle? Ot } try to get the lab to get the nanopure water working again ( I think } they need a special filter). Or would adding a little methanol help? I } am open to suggestions! Thank you. } } Login Host: 138.9.63.239 } =============================
-- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Professor /_ / __ /__ \ \ \__ Biology Department / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / ___ / \ \__/ \ ____ Amherst, MA, 01003 www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 5, 21 -- From baskin-at-bio.umass.edu Fri Jan 20 09:27:08 2012 5, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0KFR7Pt006068 5, 21 -- for {microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 09:27:07 -0600 5, 21 -- Received: from [172.30.52.170] (neutopia.bio.umass.edu [128.119.55.8]) 5, 21 -- (authenticated bits=0) 5, 21 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id q0KFR4YK000326 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 21 -- Fri, 20 Jan 2012 10:27:07 -0500 (EST) 5, 21 -- Mime-Version: 1.0 5, 21 -- Message-Id: {p06240506cb3f36157296-at-[172.30.52.170]} 5, 21 -- In-Reply-To: {201201201501.q0KF1jZr021774-at-ns.microscopy.com} 5, 21 -- References: {201201201501.q0KF1jZr021774-at-ns.microscopy.com} 5, 21 -- Date: Fri, 20 Jan 2012 10:27:03 -0500 5, 21 -- To: connellyps-at-nhlbi.nih.gov, bplowman-at-pacific.edu 5, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 5, 21 -- Subject: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks 5, 21 -- Cc: microscopy-at-microscopy.com 5, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Fri, 20 Jan 2012 10:27:07 -0500 (EST) 5, 21 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
Dear Barbara, I think this problem has to do with the pH of the water that is used. Probably the pH of the nanopure water was higher than the distilled water.
Groeten, Hans Janssen.
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: vrijdag 20 januari 2012 16:30 To: Hans Janssen
Hi, I have no experiencing with aging uranyl acetate. But am curious how time could cause a crystal to become insoluble. Many chemicals on the lab shelf absorb water over time (they often form cakes). This make them difficult to weigh out but I cannot think why it would hinder solubility. Alternatively, could an oxide be forming on the surface of the grains, an oxide might in insoluble (think rust). If the UA powder is not super fine, I guess you could try to grind it with a mortar and pestle to expose fresh surface, although from a safety point of you that seems dubious (UA aerosol -- yumm!). Anyway, I'd be interested to hear if anyone actually knows how time leads to insolubility (of powders -- not mentality, smile).
As ever Tobias
At 9:01 AM -0600 1/20/12, connellyps-at-nhlbi.nih.gov wrote: } } } Barbara, } } I had the same problem making a 2% solution about 20 years ago - } using old UA. As soon as I started using a new supply the UA went } into solution as I thought it should. } } If your UA comes in a can it is not a bad idea to store the bottle } in the can between uses. I have found that this extends the useable } life of the crystals until I empty the bottle. } } Pat } } Patricia Stranen Connelly } Research Assistant } NHLBI Electron Microscopy Core } National Institutes of Health } 14 Service Road West } Bldg. 14E - Rm. 111B MSC 5570 } Bethesda, MD 20892-5570 } Phone 301-496-3491 } FAX 301-402-0170 } connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} } } Opinions and experiences related are those of Pat Connelly and do } not represent the NIH. This message is not confidential and can be } freely shared and reproduced. } } } --------------------------------------------------------------------------- } Email: bplowman-at-pacific.edu Name: Barbara Plowman } Organization: University of the Pacific, Arthur A. Dugoni School of } Dentistry } Title-Subject: [Filtered] Uranyl acetate } } Message: I have been having a problem with getting uranyl acetate to go } into solution. I have been using the same bottle of UA for over 22 } years. I have not had any problems when I was using nanopure water, but } now that I am using distilled water, I can't seem to make a 3% solution } of UAaq. Is there anything I could do to help it go into solution? I am } near the end of the bottle. Maybe I should just buy another bottle? Ot } try to get the lab to get the nanopure water working again ( I think } they need a special filter). Or would adding a little methanol help? I } am open to suggestions! Thank you. } } Login Host: 138.9.63.239 } =============================
-- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Professor /_ / __ /__ \ \ \__ Biology Department / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / ___ / \ \__/ \ ____ Amherst, MA, 01003 www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 5, 21 -- From baskin-at-bio.umass.edu Fri Jan 20 09:27:08 2012 5, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0KFR7Pt006068 5, 21 -- for {microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 09:27:07 -0600 5, 21 -- Received: from [172.30.52.170] (neutopia.bio.umass.edu [128.119.55.8]) 5, 21 -- (authenticated bits=0) 5, 21 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id q0KFR4YK000326 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 21 -- Fri, 20 Jan 2012 10:27:07 -0500 (EST) 5, 21 -- Mime-Version: 1.0 5, 21 -- Message-Id: {p06240506cb3f36157296-at-[172.30.52.170]} 5, 21 -- In-Reply-To: {201201201501.q0KF1jZr021774-at-ns.microscopy.com} 5, 21 -- References: {201201201501.q0KF1jZr021774-at-ns.microscopy.com} 5, 21 -- Date: Fri, 20 Jan 2012 10:27:03 -0500 5, 21 -- To: connellyps-at-nhlbi.nih.gov, bplowman-at-pacific.edu 5, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 5, 21 -- Subject: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks 5, 21 -- Cc: microscopy-at-microscopy.com 5, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Fri, 20 Jan 2012 10:27:07 -0500 (EST) 5, 21 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
==============================Original Headers============================== 13, 25 -- From j.janssen-at-nki.nl Fri Jan 20 09:48:40 2012 13, 25 -- Received: from edge.nki.nl (edge-1.nki.nl [194.171.7.92]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0KFmefZ023347 13, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 09:48:40 -0600 13, 25 -- Received: from caht-2.nki.nl (194.171.7.85) by edge-1.nki.nl (194.171.7.92) 13, 25 -- with Microsoft SMTP Server (TLS) id 8.3.192.1; Fri, 20 Jan 2012 16:48:30 13, 25 -- +0100 13, 25 -- Received: from mstr-1.nki.nl ([172.31.4.101]) by caht-2.nki.nl 13, 25 -- ([172.31.4.104]) with mapi; Fri, 20 Jan 2012 16:48:39 +0100 13, 25 -- From: {j.janssen-at-nki.nl} 13, 25 -- To: {Microscopy-at-microscopy.com} 13, 25 -- Date: Fri, 20 Jan 2012 16:48:38 +0100 13, 25 -- Subject: FW: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks 13, 25 -- Thread-Topic: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks 13, 25 -- Thread-Index: AczXiFsFBo/x42P/Sb6+3eT/3N1sdwAAhRGw 13, 25 -- Message-ID: {F7BE8A0314FCF948BD9CFA10E54AEABD324D317782-at-mstr-1.nki.nl} 13, 25 -- Accept-Language: nl-NL, en-US 13, 25 -- Content-Language: en-US 13, 25 -- X-MS-Has-Attach: 13, 25 -- X-MS-TNEF-Correlator: 13, 25 -- acceptlanguage: nl-NL, en-US 13, 25 -- Content-Type: text/plain; charset="us-ascii" 13, 25 -- MIME-Version: 1.0 13, 25 -- Content-Transfer-Encoding: 8bit 13, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0KFmefZ023347 ==============================End of - Headers==============================
I know that uranyl acetate is generally considered as only slowly/poorly soluble in water. I would suspect that acetate salts would decompose more readily than some other salts and so I had always just assumed that old uranyl acetate just slowly oxidised producing a less soluble mixture.
Certainly I had heard that 10+ year old uranyl acetate was less soluble than a fresh supply so I had always refused kind gifts of uranyl acetate if it was old.
I don't know if this helps much.
Malcolm
Malcolm Haswell Imaging Suite Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
________________________________________ X-from: j.janssen-at-nki.nl [j.janssen-at-nki.nl] Sent: 20 January 2012 15:56 To: Malcolm Haswell
Dear Barbara, I think this problem has to do with the pH of the water that is used. Probably the pH of the nanopure water was higher than the distilled water.
Groeten, Hans Janssen.
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: vrijdag 20 januari 2012 16:30 To: Hans Janssen
Hi, I have no experiencing with aging uranyl acetate. But am curious how time could cause a crystal to become insoluble. Many chemicals on the lab shelf absorb water over time (they often form cakes). This make them difficult to weigh out but I cannot think why it would hinder solubility. Alternatively, could an oxide be forming on the surface of the grains, an oxide might in insoluble (think rust). If the UA powder is not super fine, I guess you could try to grind it with a mortar and pestle to expose fresh surface, although from a safety point of you that seems dubious (UA aerosol -- yumm!). Anyway, I'd be interested to hear if anyone actually knows how time leads to insolubility (of powders -- not mentality, smile).
As ever Tobias
At 9:01 AM -0600 1/20/12, connellyps-at-nhlbi.nih.gov wrote: } } } Barbara, } } I had the same problem making a 2% solution about 20 years ago - } using old UA. As soon as I started using a new supply the UA went } into solution as I thought it should. } } If your UA comes in a can it is not a bad idea to store the bottle } in the can between uses. I have found that this extends the useable } life of the crystals until I empty the bottle. } } Pat } } Patricia Stranen Connelly } Research Assistant } NHLBI Electron Microscopy Core } National Institutes of Health } 14 Service Road West } Bldg. 14E - Rm. 111B MSC 5570 } Bethesda, MD 20892-5570 } Phone 301-496-3491 } FAX 301-402-0170 } connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} } } Opinions and experiences related are those of Pat Connelly and do } not represent the NIH. This message is not confidential and can be } freely shared and reproduced. } } } --------------------------------------------------------------------------- } Email: bplowman-at-pacific.edu Name: Barbara Plowman } Organization: University of the Pacific, Arthur A. Dugoni School of } Dentistry } Title-Subject: [Filtered] Uranyl acetate } } Message: I have been having a problem with getting uranyl acetate to go } into solution. I have been using the same bottle of UA for over 22 } years. I have not had any problems when I was using nanopure water, but } now that I am using distilled water, I can't seem to make a 3% solution } of UAaq. Is there anything I could do to help it go into solution? I am } near the end of the bottle. Maybe I should just buy another bottle? Ot } try to get the lab to get the nanopure water working again ( I think } they need a special filter). Or would adding a little methanol help? I } am open to suggestions! Thank you. } } Login Host: 138.9.63.239 } =============================
-- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Professor /_ / __ /__ \ \ \__ Biology Department / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / ___ / \ \__/ \ ____ Amherst, MA, 01003 www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 5, 21 -- From baskin-at-bio.umass.edu Fri Jan 20 09:27:08 2012 5, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0KFR7Pt006068 5, 21 -- for {microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 09:27:07 -0600 5, 21 -- Received: from [172.30.52.170] (neutopia.bio.umass.edu [128.119.55.8]) 5, 21 -- (authenticated bits=0) 5, 21 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id q0KFR4YK000326 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 21 -- Fri, 20 Jan 2012 10:27:07 -0500 (EST) 5, 21 -- Mime-Version: 1.0 5, 21 -- Message-Id: {p06240506cb3f36157296-at-[172.30.52.170]} 5, 21 -- In-Reply-To: {201201201501.q0KF1jZr021774-at-ns.microscopy.com} 5, 21 -- References: {201201201501.q0KF1jZr021774-at-ns.microscopy.com} 5, 21 -- Date: Fri, 20 Jan 2012 10:27:03 -0500 5, 21 -- To: connellyps-at-nhlbi.nih.gov, bplowman-at-pacific.edu 5, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 5, 21 -- Subject: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks 5, 21 -- Cc: microscopy-at-microscopy.com 5, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Fri, 20 Jan 2012 10:27:07 -0500 (EST) 5, 21 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
==============================Original Headers============================== 13, 25 -- From j.janssen-at-nki.nl Fri Jan 20 09:48:40 2012 13, 25 -- Received: from edge.nki.nl (edge-1.nki.nl [194.171.7.92]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0KFmefZ023347 13, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 09:48:40 -0600 13, 25 -- Received: from caht-2.nki.nl (194.171.7.85) by edge-1.nki.nl (194.171.7.92) 13, 25 -- with Microsoft SMTP Server (TLS) id 8.3.192.1; Fri, 20 Jan 2012 16:48:30 13, 25 -- +0100 13, 25 -- Received: from mstr-1.nki.nl ([172.31.4.101]) by caht-2.nki.nl 13, 25 -- ([172.31.4.104]) with mapi; Fri, 20 Jan 2012 16:48:39 +0100 13, 25 -- From: {j.janssen-at-nki.nl} 13, 25 -- To: {Microscopy-at-microscopy.com} 13, 25 -- Date: Fri, 20 Jan 2012 16:48:38 +0100 13, 25 -- Subject: FW: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks 13, 25 -- Thread-Topic: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks 13, 25 -- Thread-Index: AczXiFsFBo/x42P/Sb6+3eT/3N1sdwAAhRGw 13, 25 -- Message-ID: {F7BE8A0314FCF948BD9CFA10E54AEABD324D317782-at-mstr-1.nki.nl} 13, 25 -- Accept-Language: nl-NL, en-US 13, 25 -- Content-Language: en-US 13, 25 -- X-MS-Has-Attach: 13, 25 -- X-MS-TNEF-Correlator: 13, 25 -- acceptlanguage: nl-NL, en-US 13, 25 -- Content-Type: text/plain; charset="us-ascii" 13, 25 -- MIME-Version: 1.0 13, 25 -- Content-Transfer-Encoding: 8bit 13, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0KFmefZ023347 ==============================End of - Headers==============================-- University of Sunderland - life-changing: see our new TV advert at http://www.lifechangingsunderland.com or http://www.sunderland.ac.uk
==============================Original Headers============================== 23, 35 -- From malcolm.haswell-at-sunderland.ac.uk Fri Jan 20 10:17:06 2012 23, 35 -- Received: from max1.sunderland.ac.uk (max1.sunderland.ac.uk [157.228.52.120]) 23, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q0KGH5O6008369 23, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 10:17:06 -0600 23, 35 -- Received: (qmail 26970 invoked from network); 20 Jan 2012 16:17:01 -0000 23, 35 -- Received: from localhost (127.0.0.1) 23, 35 -- by max1.sunderland.ac.uk with SMTP; 20 Jan 2012 16:17:01 -0000 23, 35 -- Received: from max1.sunderland.ac.uk ([127.0.0.1]) 23, 35 -- by localhost (max1.sunderland.ac.uk [127.0.0.1]) (amavisd-new, port 10024) 23, 35 -- with SMTP id 26249-04 for {Microscopy-at-microscopy.com} ; 23, 35 -- Fri, 20 Jan 2012 16:16:54 +0000 (GMT) 23, 35 -- Received: (qmail 26946 invoked by uid 607); 20 Jan 2012 16:16:54 -0000 23, 35 -- Received: from unknown (HELO cas-01.uni.ad.sunderland.ac.uk) (157.228.67.41) 23, 35 -- by max1.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Fri, 20 Jan 2012 16:16:54 +0000 23, 35 -- Received: from MB-01.uni.ad.sunderland.ac.uk ([169.254.1.69]) by 23, 35 -- cas-01.uni.ad.sunderland.ac.uk ([157.228.67.41]) with mapi; Fri, 20 Jan 2012 23, 35 -- 16:16:55 +0000 23, 35 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk} 23, 35 -- To: "Microscopy MSA (Microscopy-at-microscopy.com)" {Microscopy-at-microscopy.com} 23, 35 -- Subject: RE: [Microscopy] FW: Re: viaWWW:Uranyl acetate-aging rocks 23, 35 -- Thread-Topic: [Microscopy] FW: Re: viaWWW:Uranyl acetate-aging rocks 23, 35 -- Thread-Index: AQHM14whMMH/Pav+YUyJk0yhKl85F5YVa4Gu 23, 35 -- Date: Fri, 20 Jan 2012 16:16:55 +0000 23, 35 -- Message-ID: {F258EBB933D8F549902DDFC16829705D32BE0C7F-at-mb-01.uni.ad.sunderland.ac.uk} 23, 35 -- References: {201201201556.q0KFupLJ006783-at-ns.microscopy.com} 23, 35 -- In-Reply-To: {201201201556.q0KFupLJ006783-at-ns.microscopy.com} 23, 35 -- Accept-Language: en-GB, en-US 23, 35 -- Content-Language: en-GB 23, 35 -- X-MS-Has-Attach: 23, 35 -- X-MS-TNEF-Correlator: 23, 35 -- Content-Type: text/plain; charset="us-ascii" 23, 35 -- MIME-Version: 1.0 23, 35 -- X-Virus-Scanned: by iCritical at max1.sunderland.ac.uk 23, 35 -- Content-Transfer-Encoding: 8bit 23, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0KGH5O6008369 ==============================End of - Headers==============================
Our color printer, which I use to print B-W copies of TEM images of carbon black, is sounding like a rusty gate. On the chance we replace it, I'm looking for suggestions: What's going to give me the nicest B+W TEM print? Any suggestions? Money is (and when is it not?) a factor.
If you're shy, contact me directly, or not. All suggestions will be considered,
Thanks...................
Frank
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 17, 25 -- From frank_karl-at-ardl.com Fri Jan 20 10:31:07 2012 17, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0KGV6kI025440 17, 25 -- for {microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 10:31:06 -0600 17, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 17, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 20 17, 25 -- Jan 2012 11:31:06 -0500 17, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 17, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 17, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 17, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 17, 25 -- Date: Fri, 20 Jan 2012 11:31:05 -0500 17, 25 -- Subject: B+W printer 17, 25 -- Thread-Topic: B+W printer 17, 25 -- Thread-Index: AczXkOhEw3ocrFnMTq2Vs3qVUiA2sw== 17, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150770158454200-at-exchange2k7.ad.ardl.com} 17, 25 -- Accept-Language: en-US 17, 25 -- Content-Language: en-US 17, 25 -- X-MS-Has-Attach: 17, 25 -- X-MS-TNEF-Correlator: 17, 25 -- acceptlanguage: en-US 17, 25 -- Content-Type: text/plain; charset="iso-8859-1" 17, 25 -- MIME-Version: 1.0 17, 25 -- Content-Transfer-Encoding: 8bit 17, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0KGV6kI025440 ==============================End of - Headers==============================
I had used the same water source with both samples of UA , old and new. I know that the pH stayed the same most of the year so I do not think that the pH changed from morning to afternoon of the same day. If I remember correctly our pH usually ran in the 6.5 range. I know it was well below a pH of 7.0 any time I tested it.
Reply to Malcolm.
I had trouble getting the old UA to go into a 2% solution over a period of time. It just kept getting worse to the point that I had it mixing for up to two days (under a metal can to exclude the room lights) which had me questioning what was wrong when I knew that others made 4% UA with no difficulty. At that time I "borrowed" a gram from another lab which I knew was going into solution fine according to my EM Tech. friend. I then used my water, glass bottle, etc. the same that I had done before with my old UA. It went into solution within a short period of time so I ordered a new supply of UA from the same supplier of my old bottle and the borrowed UA and have not had a problem since.
I suggest that those who offer me gifts of old UA send the bottles out the next time they have a pick up of radioactive waste. I have tried several old supplies over the years and found none to go into solution readily.
Reply to Tobias.
When I started in EM back in 1971 my supply of UA was of coarse crystals which I was instructed to grind fine. I had a dedicated mortar and pestle for this purpose. I was very glad that when I ordered my first new bottle of UA some years later that it came as a fine, almost powder like grade of crystals. This is how my UA has come since that time.
Since it is now 2012 and I am still alive I guess I did a good job of not inhaling the dust! According to a very old edition of the Merc Index, UA was used as a snuff. Hard to believe with all the cautions on current MSDS sheets.
Pat Connelly
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Well, this has been an interesting discussion about what happens with aging uranyl acetate (UAc) salts.
Although I am certainly not a chemist, I suspect several events (some already mentioned by other contributors) are taking place to cause the salts to become less soluble:
1. Degradation of acetate. Acetates are notoriously unstable and break down with time. If you detect a strong smell of acetic acid (carefully, by wafting the air above the container), then decomposition is taking place.
2. Phytolytic decomposition. Most people keep the UAc away from the light; however, this is not always the case. I've seen solutions sitting on top of counters for long periods of time in clear, glass containers. You should cover the container with aluminum foil and keep it refrigerated (to cut down on light even more).
3. Radiolytic decomposition. UAc (even depleted, U238 Ac) is weakly radioactive, giving off alpha, but also beta particles and gamma rays as part of the decay process. Over time, these will degrade most chemical compounds. In fact, ever wonder what was coating the inside of your UAc staining vessel? That insoluble material is uranyl oxide (JIÃ TEPLÃ & VÃCLAV TULÃK. 1963. Formation of Peroxidic Precipitate in the Radiolysis of Uranyl Nitrate Ketone Solutions. Nature 200: 671-672).
Now, for some "popular" trivia. Anyone ever hear of a Revigator?
That's a water vessel lined with uranium that releases radon into the water! Back in the 1920's they were sold to fitness folks to "restore water's lost element" and invigorate the body……… Frightening! You can occasionally find them on EBay and I am surprised they are even allowed to be sold.
-- John J. Bozzola, Ph.D., (Happily Retired) Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 9, 18 -- From bozzola-at-siu.edu Fri Jan 20 14:38:09 2012 9, 18 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0KKc9Xa002115 9, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 14:38:09 -0600 9, 18 -- Received: by vcbfl11 with SMTP id fl11so788462vcb.0 9, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 12:38:09 -0800 (PST) 9, 18 -- MIME-Version: 1.0 9, 18 -- Received: by 10.52.67.229 with SMTP id q5mr15511306vdt.14.1327091889129; Fri, 9, 18 -- 20 Jan 2012 12:38:09 -0800 (PST) 9, 18 -- Received: by 10.52.188.200 with HTTP; Fri, 20 Jan 2012 12:38:08 -0800 (PST) 9, 18 -- Date: Fri, 20 Jan 2012 14:38:08 -0600 9, 18 -- Message-ID: {CAKeCJ9xPNt=RGJJ5rQY0rZ4=9ZXcVfZwo=eMYF6_fckTsz=cwg-at-mail.gmail.com} 9, 18 -- Subject: Re: Uranyl acetate-aging rocks 9, 18 -- From: John Bozzola {bozzola-at-siu.edu} 9, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 9, 18 -- Content-Type: text/plain; charset=UTF-8 9, 18 -- Content-Transfer-Encoding: 8bit 9, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0KKc9Xa002115 ==============================End of - Headers==============================
It looks like I am becoming illegally blind: I cannot find instructions for authors and/or abstract template on M&M site. Recommended in Call for Papers link does not connect to the right page.
Were there any changes in template or I can use my old abstract as a template?
Thanks,
Vladimir
==============================Original Headers============================== 6, 29 -- From DusevichV-at-umkc.edu Fri Jan 20 15:40:32 2012 6, 29 -- Received: from mxnip01.um.umsystem.edu (mxnip01.um.umsystem.edu [209.106.229.21]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0KLeWh7001842 6, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2012 15:40:32 -0600 6, 29 -- X-IronPort-Anti-Spam-Filtered: true 6, 29 -- X-IronPort-Anti-Spam-Result: Av0EANreGU/PoJ7Q/2dsb2JhbABDrgqBBYFzAQEEHR1PAgEqFBAyJQIEG7dIiQWLQ2MEp2Q 6, 29 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) 6, 29 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Jan 2012 15:40:32 -0600 6, 29 -- Received: from UM-MBX-T01.um.umsystem.edu ([169.254.1.15]) by 6, 29 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi id 14.01.0355.002; 6, 29 -- Fri, 20 Jan 2012 15:40:32 -0600 6, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 6, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 29 -- Subject: M&M 2012 - instructions for authors? 6, 29 -- Thread-Topic: M&M 2012 - instructions for authors? 6, 29 -- Thread-Index: AQHM17wih2AQO5DvEkGcYPfZl8WL1w== 6, 29 -- Date: Fri, 20 Jan 2012 21:40:31 +0000 6, 29 -- Message-ID: {B1F9B7FAC2452540B964F5E9DB582806015501-at-UM-MBX-T01.um.umsystem.edu} 6, 29 -- References: {201112162120.pBGLKP0H003964-at-ns.microscopy.com} 6, 29 -- In-Reply-To: {201112162120.pBGLKP0H003964-at-ns.microscopy.com} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- x-originating-ip: [134.193.157.168] 6, 29 -- Content-Type: text/plain; charset="us-ascii" 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0KLeWh7001842 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bplowman-at-pacific.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bplowman-at-pacific.edu Name: Barbara L. Plowman
Organization: University of the Pacific, Arthur A. Dugoni School of Dentistry
Title-Subject: [Filtered] Uranyl acetate
Message: Thanks for all the information regarding uranyl acetate. I'm purchasing a new bottle of it! Barbara
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-gaugler.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-gaugler.com Name: Dr Gary Gaugler
Title-Subject: [Filtered] Hitachi S-570 docs
Message: A kind person sent me a complete set of docs with schematics for this SEM. Now it seems that I cannot use them, having been trying to move to a later system.
Can someone use these docs? First come, first served. No charge. This is a huge data dump...originals.
Note: I have already sent the O.P. Charles Humphrey's Alcian Blue method (which he notes was published by Nermut in 1982).
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************************** } Date: Mon, 23 Jan 2012 05:54:13 -0800 } From: {AssociationManagement-at-microscopy.org} } Reply-To: Amanda {lever.amanda1-at-gmail.com} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Monday, January 23, } 2012 at 05:53:59 AM. } } realname - Amanda } Email - lever.amanda1-at-gmail.com } ORGANIZATION - Draper Laboratory } EDUCATION - Graduate College } LOCATION - Boston, MA } SUBJECT_OF_QUESTION - Transmission Electron Microscopy } QUESTION - I am currently using a UA negative stain on carbon grids } to visualize and image liposomes. I have been noticing heterogeniety } across the EM grids (some liposomes look nice and maintain the 3D } shape while others appear flat and 2D in shape). Why might this be } happening and are there any techniques to prevent it ? }
==============================Original Headers============================== 2, 24 -- From oshel1pe-at-cmich.edu Mon Jan 23 08:09:44 2012 2, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NE9i54028791 2, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 08:09:44 -0600 2, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 2, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q0NE97Mj015496 2, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 09:09:43 -0500 2, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 2, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Mon, 23 Jan 2, 24 -- 2012 09:09:36 -0500 2, 24 -- MIME-Version: 1.0 2, 24 -- Message-ID: {a06240807cb431a2f538e-at-[141.209.160.249]} 2, 24 -- Date: Mon, 23 Jan 2012 09:09:34 -0500 2, 24 -- To: {Microscopy-at-microscopy.com} 2, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 24 -- Subject: uneven negative staining of liposomes 2, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 2, 24 -- X-Originating-IP: [141.209.160.249] 2, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) 2, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 2, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 24 -- X-CanItPRO-Stream: default 2, 24 -- X-Canit-Stats-ID: 07Gp29H5Q - bf6309fc894d - 20120123 2, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Note: I have referred the O.P. to MSA's surplus equipment page, but anything specific to what he's looking for would help him.
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************************** } realname - Ralph Appy } Email - r.appy-at-cox.net } ORGANIZATION - Cabrillo Marine Aquarium } LOCATION - San Pedro, CA, U.S.A. } SUBJECT_OF_QUESTION - New/Refurbished Microscope } QUESTION - I am a retired environmental scientist and rekindling my } interest in fish parasitology at a local marine aquarium. I would } like to find a used/refurbished phase contrast microscope with a } drawing tube. Not sure if any members might have such a surplus } microscope. Not having much luck on what I want on the internet. I } know this is an unusual request. I am bonafide [...just published } in J Parasitol 97(6):1035-1048] and not indepentently wealthy. } } Ralph
==============================Original Headers============================== 3, 24 -- From oshel1pe-at-cmich.edu Mon Jan 23 08:46:46 2012 3, 24 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NEkkL7031074 3, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 08:46:46 -0600 3, 24 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 3, 24 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q0NEkjUG005311 3, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 09:46:45 -0500 3, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 3, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Mon, 23 Jan 3, 24 -- 2012 09:46:04 -0500 3, 24 -- MIME-Version: 1.0 3, 24 -- Message-ID: {a06240804cb431011f49e-at-[141.209.160.249]} 3, 24 -- Date: Mon, 23 Jan 2012 09:46:01 -0500 3, 24 -- To: {Microscopy-at-microscopy.com} 3, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 24 -- Subject: Used phase contrast microscope wanted 3, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 3, 24 -- X-Originating-IP: [141.209.160.249] 3, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) 3, 24 -- X-Spam-Score: 0.00 () [Hold at 6.00] _L_IAA,_L_LLEXCH,Bayes(0.0001:-0.5) 3, 24 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 3, 24 -- X-CanItPRO-Stream: default 3, 24 -- X-Canit-Stats-ID: 06Gp2KJlW - 51ac8f85fcdf - 20120123 3, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
The Hooke College of Applied Sciences, located in Westmont, IL, is offering its transmission electron microscopy short course March 6-8, 2012. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment. For further details and registration information, please follow the link below:
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
==============================Original Headers============================== 12, 27 -- From eschumacher-at-mccrone.com Mon Jan 23 09:54:13 2012 12, 27 -- Received: from mail.mccrone.com (mail.mccrone.com [12.54.22.114]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NFsDR6016906 12, 27 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 09:54:13 -0600 12, 27 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by 12, 27 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Mon, 23 Jan 2012 09:54:11 12, 27 -- -0600 12, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 12, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 27 -- CC: Christine Gorman {CGorman-at-hookecollege.com} 12, 27 -- Date: Mon, 23 Jan 2012 09:54:10 -0600 12, 27 -- Subject: Short Course Announcement: TEM 12, 27 -- Thread-Topic: Short Course Announcement: TEM 12, 27 -- Thread-Index: AczZ5z8UaEJjCF++TTeqmGp9Qyk00w== 12, 27 -- Message-ID: {874B1DB532886444A60A015EE363493F5E5D8A3F87-at-mccronemsg07.tmg.mccrone.com} 12, 27 -- Accept-Language: en-US 12, 27 -- Content-Language: en-US 12, 27 -- X-MS-Has-Attach: 12, 27 -- X-MS-TNEF-Correlator: 12, 27 -- x-vipre-scanned: 009BFF6C002CB1009C00B9 12, 27 -- x-ninja-pim: Scanned by Ninja 12, 27 -- x-ninja-attachmentfiltering: (no action) 12, 27 -- acceptlanguage: en-US 12, 27 -- Content-Type: text/plain; charset="us-ascii" 12, 27 -- MIME-Version: 1.0 12, 27 -- Content-Transfer-Encoding: 8bit 12, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0NFsDR6016906 ==============================End of - Headers==============================
I have a sample of zinc borate that I've been asked to determine the atomic number ratio of boron to zinc. Has anybody had any luck in detecting boron and if so, how did you go about it? I have IXRF software using an Amray 1830 with a light element detector. Is it a matter of setting up the detector threshold so you can see below carbon or what? Any help would be greatly appreciated!
Don Kierstead Microscopist ARDL, Inc. www.ardl.com (866) 778-ARDL [toll free] (330) 794-6600 [worldwide] (330) 794-6610 [fax]
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 5, 25 -- From donk-at-ardl.com Mon Jan 23 10:41:28 2012 5, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NGfRs9002523 5, 25 -- for {Microscopy-at-Microscopy.com} ; Mon, 23 Jan 2012 10:41:28 -0600 5, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 5, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Mon, 23 5, 25 -- Jan 2012 11:41:27 -0500 5, 25 -- From: Don Kierstead {donk-at-ardl.com} 5, 25 -- To: "'Microscopy-at-Microscopy.com'" {Microscopy-at-Microscopy.com} 5, 25 -- Disposition-Notification-To: Don Kierstead {donk-at-ardl.com} 5, 25 -- Return-Receipt-To: {donk-at-ardl.com} 5, 25 -- Date: Mon, 23 Jan 2012 11:41:26 -0500 5, 25 -- Subject: RE: Detection of Boron by EDX 5, 25 -- Thread-Topic: RE: Detection of Boron by EDX 5, 25 -- Thread-Index: AczZ7dmufI5JWib0QSC9//1W3YRy2w== 5, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150770158449924-at-exchange2k7.ad.ardl.com} 5, 25 -- Accept-Language: en-US 5, 25 -- Content-Language: en-US 5, 25 -- X-MS-Has-Attach: 5, 25 -- X-MS-TNEF-Correlator: 5, 25 -- acceptlanguage: en-US 5, 25 -- Content-Type: text/plain; charset="iso-8859-1" 5, 25 -- MIME-Version: 1.0 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0NGfRs9002523 ==============================End of - Headers==============================
The first thing you do is proceed with great caution. If your system can detect boron, I am sure it will spit out a number. It might even have some resemblance to the truth, but probably not.
The second thing you should probably do is start looking into the difficulty of light element analysis. You have boron and oxygen (light elements) combined with zinc (a rather heavy element). Look into the ZAF factors and see how big the absorption corrections. Ask yourself how well you know the mass absorption coefficients for those combinations. See what you can do about changing conditions (dropping voltage) to reduce the magnitude of the corrections.
The third thing you do would be to make sure your system can see the boron peak. My guess is that you are probably not optimized to detect boron. We had an old Kevex Quantum detector running on one of the early IXRF systems. You might be able to adjust your discriminators to better see the peak. We had to. In the end, we could see most of the boron peak, but not much if any of the background on the low energy side.
The fourth thing would be to get some reference materials. If your system cannot give you right answers on a known compound (and it probably won't), then you can forget about trying to analyze an unknown compound. You may be able to collect your own standards to help the matter, but that is not a job for novices. (If you succeed, you may have progressed beyond the novice stage. There is much more to be said on this topic.)
Another thing is to consider the limitations of EDS in general. I suppose they will not have a flat, polished sample. You'll need to consider the effects of geometry. They affect results and become more significant the lighter the elements. Depending on where you hit a particle (on the side near the detector or the side away), you can probably produce any results you want.
Finally, I would get a large supply of salt so you can hand out your results with many grains. Seriously, I would try to get your client to accept qualitative results such as "The sample shows Zn, B, and O. It could be borate." This will be even more convincing if you can compare spectra of their unknown to a known sample of zinc borate and say, "See, they match". That may be as far as you really want to push it.
Regards, Warren Straszheim
-----Original Message----- X-from: donk-at-ardl.com [mailto:donk-at-ardl.com] Sent: Monday, January 23, 2012 10:42 AM To: wesaia-at-iastate.edu
I have a sample of zinc borate that I've been asked to determine the atomic number ratio of boron to zinc. Has anybody had any luck in detecting boron and if so, how did you go about it? I have IXRF software using an Amray 1830 with a light element detector. Is it a matter of setting up the detector threshold so you can see below carbon or what? Any help would be greatly appreciated!
Don Kierstead Microscopist ARDL, Inc. www.ardl.com (866) 778-ARDL [toll free] (330) 794-6600 [worldwide] (330) 794-6610 [fax]
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 5, 25 -- From donk-at-ardl.com Mon Jan 23 10:41:28 2012 5, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NGfRs9002523 5, 25 -- for {Microscopy-at-Microscopy.com} ; Mon, 23 Jan 2012 10:41:28 -0600 5, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 5, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Mon, 23 5, 25 -- Jan 2012 11:41:27 -0500 5, 25 -- From: Don Kierstead {donk-at-ardl.com} 5, 25 -- To: "'Microscopy-at-Microscopy.com'" {Microscopy-at-Microscopy.com} 5, 25 -- Disposition-Notification-To: Don Kierstead {donk-at-ardl.com} 5, 25 -- Return-Receipt-To: {donk-at-ardl.com} 5, 25 -- Date: Mon, 23 Jan 2012 11:41:26 -0500 5, 25 -- Subject: RE: Detection of Boron by EDX 5, 25 -- Thread-Topic: RE: Detection of Boron by EDX 5, 25 -- Thread-Index: AczZ7dmufI5JWib0QSC9//1W3YRy2w== 5, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150770158449924-at-exchange2k7.ad.ardl.com} 5, 25 -- Accept-Language: en-US 5, 25 -- Content-Language: en-US 5, 25 -- X-MS-Has-Attach: 5, 25 -- X-MS-TNEF-Correlator: 5, 25 -- acceptlanguage: en-US 5, 25 -- Content-Type: text/plain; charset="iso-8859-1" 5, 25 -- MIME-Version: 1.0 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0NGfRs9002523 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 34 -- From wesaia-at-iastate.edu Mon Jan 23 11:11:52 2012 18, 34 -- Received: from mailexch-1.iastate.edu (mailexch-1.iastate.edu [129.186.140.21]) 18, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NHBq20019854 18, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 11:11:52 -0600 18, 34 -- Received: from ITSEDGE1D.its.iastate.edu (itsedge1d.its.iastate.edu [10.10.82.5]) 18, 34 -- by mailexch-1.iastate.edu (8.14.4/8.12.10) with ESMTP id q0NHBd1V011211 18, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 11:11:51 -0600 18, 34 -- Received: from ITSHUB3A.its.iastate.edu (10.10.83.9) by 18, 34 -- ITSEDGE1D.its.iastate.edu (10.10.82.5) with Microsoft SMTP Server (TLS) id 18, 34 -- 14.1.339.1; Mon, 23 Jan 2012 11:11:47 -0600 18, 34 -- Received: from ITSDAG4A.its.iastate.edu ([169.254.4.104]) by 18, 34 -- ITSHUB3A.its.iastate.edu ([10.10.83.9]) with mapi id 14.01.0339.001; Mon, 23 18, 34 -- Jan 2012 11:11:46 -0600 18, 34 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 18, 34 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 18, 34 -- Subject: RE: [Microscopy] RE: Detection of Boron by EDX 18, 34 -- Thread-Topic: [Microscopy] RE: Detection of Boron by EDX 18, 34 -- Thread-Index: AQHM2e3r24qi3lgua0evERtZTdMvwJYaKySQ 18, 34 -- Date: Mon, 23 Jan 2012 17:11:45 +0000 18, 34 -- Message-ID: {0ED4049522D32A45AD4183B34807322B01AB49-at-ITSDAG4A.its.iastate.edu} 18, 34 -- References: {201201231641.q0NGfs1a003393-at-ns.microscopy.com} 18, 34 -- In-Reply-To: {201201231641.q0NGfs1a003393-at-ns.microscopy.com} 18, 34 -- Accept-Language: en-US 18, 34 -- Content-Language: en-US 18, 34 -- X-MS-Has-Attach: 18, 34 -- X-MS-TNEF-Correlator: 18, 34 -- x-originating-ip: [10.64.191.29] 18, 34 -- Content-Type: text/plain; charset="us-ascii" 18, 34 -- MIME-Version: 1.0 18, 34 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2012.1.23.165716 18, 34 -- X-ISUMailhub-test: Gauge=XXIII, Probability=23%, Report=' 18, 34 -- RATWARE_RCVD_BONUS_SPC 2.0, TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, BODY_SIZE_6000_6999 0, BODY_SIZE_7000_LESS 0, FROM_NAME_PHRASE 0, RDNS_NXDOMAIN 0, RDNS_SUSP 0, RDNS_SUSP_GENERIC 0, WEBMAIL_SOURCE 0, WEBMAIL_XOIP 0, WEBMAIL_X_IP_HDR 0, __ANY_URI 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOIP 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS ' 18, 34 -- Content-Transfer-Encoding: 8bit 18, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0NHBq20019854 ==============================End of - Headers==============================
I believe I would first consider surface tension as the culprit. Are all liposomes identically resistant to the passage of the air-water interface?
Hope this at least serves as a last resort,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
If one looks long enough, one may actually see something frozen in time that flashes one's mind to a new state. 1. The day that I asked myself how the hydrogen atom's mass could actually be: 1.0008. 2. The day, while looking at a slide of some tissue, I saw a leukocyte that had been caught sneaking in or out of a capillary: http://www.annualreviews.org/doi/abs/10.1146/annurev-pathol-011110-130224
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Monday, January 23, 2012 9:18 AM To: Monson, Frederick
Note: I have already sent the O.P. Charles Humphrey's Alcian Blue method (which he notes was published by Nermut in 1982).
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************************** } Date: Mon, 23 Jan 2012 05:54:13 -0800 } From: {AssociationManagement-at-microscopy.org} } Reply-To: Amanda {lever.amanda1-at-gmail.com} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Monday, January 23, } 2012 at 05:53:59 AM. } } realname - Amanda } Email - lever.amanda1-at-gmail.com } ORGANIZATION - Draper Laboratory } EDUCATION - Graduate College } LOCATION - Boston, MA } SUBJECT_OF_QUESTION - Transmission Electron Microscopy QUESTION - I am } currently using a UA negative stain on carbon grids to visualize and } image liposomes. I have been noticing heterogeniety across the EM grids } (some liposomes look nice and maintain the 3D shape while others appear } flat and 2D in shape). Why might this be happening and are there any } techniques to prevent it ? }
==============================Original Headers============================== 2, 24 -- From oshel1pe-at-cmich.edu Mon Jan 23 08:09:44 2012 2, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NE9i54028791 2, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 08:09:44 -0600 2, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 2, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q0NE97Mj015496 2, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 09:09:43 -0500 2, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 2, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Mon, 23 Jan 2, 24 -- 2012 09:09:36 -0500 2, 24 -- MIME-Version: 1.0 2, 24 -- Message-ID: {a06240807cb431a2f538e-at-[141.209.160.249]} 2, 24 -- Date: Mon, 23 Jan 2012 09:09:34 -0500 2, 24 -- To: {Microscopy-at-microscopy.com} 2, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 24 -- Subject: uneven negative staining of liposomes 2, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 2, 24 -- X-Originating-IP: [141.209.160.249] 2, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) 2, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 2, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 24 -- X-CanItPRO-Stream: default 2, 24 -- X-Canit-Stats-ID: 07Gp29H5Q - bf6309fc894d - 20120123 2, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 14, 37 -- From FMonson-at-wcupa.edu Mon Jan 23 15:07:05 2012 14, 37 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.117.250]) 14, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NL75vj008120 14, 37 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 15:07:05 -0600 14, 37 -- X-ASG-Debug-ID: 1327352824-0368bc119f18f70001-4CH8be 14, 37 -- Received: from WCU-XCH-04.PASSHE.LCL (wcu-xch-04.wcupa.edu [144.26.0.88]) by MX01.WCUPA.EDU with ESMTP id 3miaBb7X5rfHRStX (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Mon, 23 Jan 2012 16:07:04 -0500 (EST) 14, 37 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 14, 37 -- X-Barracuda-Apparent-Source-IP: 144.26.0.88 14, 37 -- X-ASG-Whitelist: Client 14, 37 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 14, 37 -- WCU-XCH-04.PASSHE.LCL ([fe80::58fd:1d03:b92e:36bd%15]) with mapi id 14, 37 -- 14.01.0270.001; Mon, 23 Jan 2012 16:07:04 -0500 14, 37 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 14, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 37 -- CC: "oshel1pe-at-cmich.edu" {oshel1pe-at-cmich.edu} 14, 37 -- Subject: RE: [Microscopy] uneven negative staining of liposomes 14, 37 -- Thread-Topic: [Microscopy] uneven negative staining of liposomes 14, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] uneven negative staining of liposomes 14, 37 -- Thread-Index: AQHM2dnb19mpIRTsoUSxGEknSQ49JpYacaZw 14, 37 -- Date: Mon, 23 Jan 2012 21:07:03 +0000 14, 37 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C12957A-at-WCU-XCH-03.PASSHE.LCL} 14, 37 -- References: {201201231418.q0NEIJjh024099-at-ns.microscopy.com} 14, 37 -- In-Reply-To: {201201231418.q0NEIJjh024099-at-ns.microscopy.com} 14, 37 -- Accept-Language: en-US 14, 37 -- Content-Language: en-US 14, 37 -- X-MS-Has-Attach: 14, 37 -- X-MS-TNEF-Correlator: 14, 37 -- x-originating-ip: [10.28.57.79] 14, 37 -- Content-Type: text/plain; charset="us-ascii" 14, 37 -- MIME-Version: 1.0 14, 37 -- X-Barracuda-Connect: wcu-xch-04.wcupa.edu[144.26.0.88] 14, 37 -- X-Barracuda-Start-Time: 1327352824 14, 37 -- X-Barracuda-Encrypted: AES128-SHA 14, 37 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 14, 37 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 14, 37 -- Content-Transfer-Encoding: 8bit 14, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0NL75vj008120 ==============================End of - Headers==============================
I've found a couple of papers that list Pt-blue as an alternative to Uranyl Acetate, but I haven't been able to track down the way to make it. Â Does anyone have a working recipe as how to make the Pt-blue? Â Thanks for any help/tips.
I have for a long time been keen to find references to EMs in movies and put them on a website for all to see. Every time I think about it I promptly forget and the cycle repeats not this time!
I wondered is anyone has spotted EMs in movies/tv shows/documentaries? If so what and where? If you can point out the make, model and any inaccuracies all the better!
So far the ones I can remember are:
1. Flash Gordon 1936 (Controls of the ship are a TEM) 2. Independence Day in Area 51 scene 3. The Man in White Suit 4. Predator 2 5. CSI had a Hitachi S3400 once I recall 6. There was a fake one in Spiderman 2 7. Blade Runner
Any more would be greatly appreciated including those not in English!
Regards John
.
==============================Original Headers============================== 9, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 15:59:16 2012 9, 29 -- Received: from mail-ww0-f49.google.com (mail-ww0-f49.google.com [74.125.82.49]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NLxGtL010271 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 15:59:16 -0600 9, 29 -- Received: by wgbds1 with SMTP id ds1so1372983wgb.18 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 13:59:15 -0800 (PST) 9, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 29 -- d=gmail.com; s=gamma; 9, 29 -- h=message-id:date:from:user-agent:mime-version:to:subject 9, 29 -- :content-type:content-transfer-encoding; 9, 29 -- bh=Ba4Sdz+tb/IFyn9mxcOj+s3UNCp0MilqOz/HQ13Ssj8=; 9, 29 -- b=L8Ld1beKC4vs5R3bY4RCUhI0X0RMXmT2MuJuH1/WpJTViudtsP70vIPIVirnCob/sc 9, 29 -- Rbnz27D1oogAIyvhuRWv/ADcZNe/CJg8vsMPlRBcqgTZJnne4dy/xSrZAmTNjqXWAp2f 9, 29 -- UxwFAe6RdM4JSInulGqbxS63niVp0N/bhiVuc= 9, 29 -- Received: by 10.180.95.1 with SMTP id dg1mr8530030wib.21.1327355955629; 9, 29 -- Mon, 23 Jan 2012 13:59:15 -0800 (PST) 9, 29 -- Received: from [127.0.0.1] ([87.114.247.49]) 9, 29 -- by mx.google.com with ESMTPS id n3sm45503712wiz.9.2012.01.23.13.59.14 9, 29 -- (version=TLSv1/SSLv3 cipher=OTHER); 9, 29 -- Mon, 23 Jan 2012 13:59:14 -0800 (PST) 9, 29 -- Message-ID: {4F1DD832.1070806-at-gmail.com} 9, 29 -- Date: Mon, 23 Jan 2012 21:59:14 +0000 9, 29 -- From: John Mitchels {john.mitchels-at-gmail.com} 9, 29 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:9.0) Gecko/20111222 Thunderbird/9.0.1 9, 29 -- MIME-Version: 1.0 9, 29 -- To: Microscopy-at-microscopy.com 9, 29 -- Subject: EMs in the Movies 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Quincy. In a big voice, "Let's put it in the scanning electron microscope." I was told that the consultant was an ETEC customer. I always thought it was great because I knew what Quincy was talking about (even if he didn't).
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: john.mitchels-at-gmail.com [mailto:john.mitchels-at-gmail.com] Sent: Monday, January 23, 2012 5:01 PM To: kenconverse-at-qualityimages.biz
Hi Listers
I have for a long time been keen to find references to EMs in movies and put them on a website for all to see. Every time I think about it I promptly forget and the cycle repeats not this time!
I wondered is anyone has spotted EMs in movies/tv shows/documentaries? If so what and where? If you can point out the make, model and any inaccuracies all the better!
So far the ones I can remember are:
1. Flash Gordon 1936 (Controls of the ship are a TEM) 2. Independence Day in Area 51 scene 3. The Man in White Suit 4. Predator 2 5. CSI had a Hitachi S3400 once I recall 6. There was a fake one in Spiderman 2 7. Blade Runner
Any more would be greatly appreciated including those not in English!
Regards John
.
==============================Original Headers============================== 9, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 15:59:16 2012 9, 29 -- Received: from mail-ww0-f49.google.com (mail-ww0-f49.google.com [74.125.82.49]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NLxGtL010271 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 15:59:16 -0600 9, 29 -- Received: by wgbds1 with SMTP id ds1so1372983wgb.18 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 13:59:15 -0800 (PST) 9, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 29 -- d=gmail.com; s=gamma; 9, 29 -- h=message-id:date:from:user-agent:mime-version:to:subject 9, 29 -- :content-type:content-transfer-encoding; 9, 29 -- bh=Ba4Sdz+tb/IFyn9mxcOj+s3UNCp0MilqOz/HQ13Ssj8=; 9, 29 -- b=L8Ld1beKC4vs5R3bY4RCUhI0X0RMXmT2MuJuH1/WpJTViudtsP70vIPIVirnCob/sc 9, 29 -- Rbnz27D1oogAIyvhuRWv/ADcZNe/CJg8vsMPlRBcqgTZJnne4dy/xSrZAmTNjqXWAp2f 9, 29 -- UxwFAe6RdM4JSInulGqbxS63niVp0N/bhiVuc= 9, 29 -- Received: by 10.180.95.1 with SMTP id dg1mr8530030wib.21.1327355955629; 9, 29 -- Mon, 23 Jan 2012 13:59:15 -0800 (PST) 9, 29 -- Received: from [127.0.0.1] ([87.114.247.49]) 9, 29 -- by mx.google.com with ESMTPS id n3sm45503712wiz.9.2012.01.23.13.59.14 9, 29 -- (version=TLSv1/SSLv3 cipher=OTHER); 9, 29 -- Mon, 23 Jan 2012 13:59:14 -0800 (PST) 9, 29 -- Message-ID: {4F1DD832.1070806-at-gmail.com} 9, 29 -- Date: Mon, 23 Jan 2012 21:59:14 +0000 9, 29 -- From: John Mitchels {john.mitchels-at-gmail.com} 9, 29 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:9.0) Gecko/20111222 Thunderbird/9.0.1 9, 29 -- MIME-Version: 1.0 9, 29 -- To: Microscopy-at-microscopy.com 9, 29 -- Subject: EMs in the Movies 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 28 -- From kenconverse-at-qualityimages.biz Mon Jan 23 16:28:02 2012 19, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NMS2LU027550 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 16:28:02 -0600 19, 28 -- X-Authority-Analysis: v=2.0 cv=adPjbGUt c=1 sm=0 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=tgjCbAcPFfcA:10 a=kj9zAlcOel0A:10 a=pGLkceISAAAA:8 a=Zx37jsudAAAA:8 a=1XWaLZrsAAAA:8 a=R9u4sQZRQ8pZ1kbdfDcA:9 a=G2pNGVlq4K9XA1TZV3MA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=RyjLG6jaqdoA:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=GED_LIiEBe0A:10 a=632__J-Oa152PHfi:21 a=luEdQ3gVfp5U26fh:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 19, 28 -- X-Cloudmark-Score: 0 19, 28 -- X-Originating-IP: 72.224.149.57 19, 28 -- Received: from [72.224.149.57] ([72.224.149.57:3411] helo=Ken) 19, 28 -- by cdptpa-oedge02.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 19, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 19, 28 -- id EC/D4-15128-1FEDD1F4; Mon, 23 Jan 2012 22:28:02 +0000 19, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 19, 28 -- To: {john.mitchels-at-gmail.com} , "MSA Listserver" {microscopy-at-microscopy.com} 19, 28 -- Subject: RE: [Microscopy] EMs in the Movies 19, 28 -- Date: Mon, 23 Jan 2012 17:27:59 -0500 19, 28 -- Message-ID: {15E8ACBCFD234B21A6D666E208E7DD32-at-Ken} 19, 28 -- MIME-Version: 1.0 19, 28 -- Content-Type: text/plain; 19, 28 -- charset="US-ASCII" 19, 28 -- X-Priority: 3 (Normal) 19, 28 -- X-MSMail-Priority: Normal 19, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 19, 28 -- Importance: Normal 19, 28 -- Thread-Index: AczaGnHK2IQtzxdVSz6My2nInidbYwAA0iEg 19, 28 -- In-Reply-To: {201201232200.q0NM0fnG013612-at-ns.microscopy.com} 19, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 19, 28 -- Content-Transfer-Encoding: 8bit 19, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0NMS2LU027550 ==============================End of - Headers==============================
So far thanks to over 60 responses (and a quick trawl of the archives), this is great keep them coming!
We are up to the following:
1. Flash Gordon 2. Independence Day 3. The Man in White Suit 4. Predator 2 5. CSI 6. Spiderman 2 7. Blade Runner 8. NCIS 9. Dexter 10.Contagion 11. Andromeda Strain The Andromeda Strain (1971)and then the remake 2008 tv-miniseries. 12. Quincy 13. X-Files 14.Pres Obama at Intel's Titan 15.Spiderman 1 16.American Pickers 17.The Last Mimzy 18 ALTO MEDIA 'travel' into the matter. 19. special effects for one of the Star Trek 20. Batman 21. Jurassic Park 22. The Bone Collector" analyzes something 23. Avatar 24. The Prisoner (British TV series) 25. The Naked Gun
Thanks for all the responses so far! Once collected I will put together a website and send the link to the list. John
==============================Original Headers============================== 5, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 16:58:38 2012 5, 29 -- Received: from mail-ww0-f49.google.com (mail-ww0-f49.google.com [74.125.82.49]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NMwbVi012478 5, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 16:58:38 -0600 5, 29 -- Received: by wgbds1 with SMTP id ds1so1409132wgb.18 5, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 14:58:37 -0800 (PST) 5, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 29 -- d=gmail.com; s=gamma; 5, 29 -- h=message-id:date:from:user-agent:mime-version:to:subject 5, 29 -- :content-type:content-transfer-encoding; 5, 29 -- bh=aP2U2a3GEXIy3Ac+0sIUQE7PlyU64nc7MJmzVqtaIKI=; 5, 29 -- b=HBBmIAxh9wDubCjU7tXHA3VAcCgvt3zA6GSrp+oXwasUpEBcMC2nb+FHcDzGhwo173 5, 29 -- QmRjUy/jI+ut4ZSU/SNJIHtsxVxzr4W+SRnm9GN8KTLdrYZ6fUcfhVQz2VwBuwDiyX9V 5, 29 -- ka4eL/Eker+5DBKiRgbLPoEqWbS8bSUGB92TY= 5, 29 -- Received: by 10.180.82.41 with SMTP id f9mr16708034wiy.7.1327359517654; 5, 29 -- Mon, 23 Jan 2012 14:58:37 -0800 (PST) 5, 29 -- Received: from [127.0.0.1] ([87.114.247.49]) 5, 29 -- by mx.google.com with ESMTPS id n5sm27077735wiw.7.2012.01.23.14.58.36 5, 29 -- (version=TLSv1/SSLv3 cipher=OTHER); 5, 29 -- Mon, 23 Jan 2012 14:58:36 -0800 (PST) 5, 29 -- Message-ID: {4F1DE61C.5030804-at-gmail.com} 5, 29 -- Date: Mon, 23 Jan 2012 22:58:36 +0000 5, 29 -- From: John Mitchels {john.mitchels-at-gmail.com} 5, 29 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:9.0) Gecko/20111222 Thunderbird/9.0.1 5, 29 -- MIME-Version: 1.0 5, 29 -- To: Microscopy-at-microscopy.com 5, 29 -- Subject: Updated Movie List 5, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We have a Leo 1550 FEG-SEM that isn't running as well as it should be. We're not quite sure what the problem is exactly, but we know what the results are.
A few things are not fully functioning as our beam current is on average in the low 40 micro-amp range. Another issue is the alignment of the beam to the aperatures. When we select different apertures, the beam is supposed to be calibrated to the exact coordinates of the aperture and be deflected through the one selected. This is not happening though, instead we get a beam that moves slightly off from where it was originally, which in our case seems to be at the 30um aperture.
Does anyone out there have any experience to these columns and know of any kind of solution to these problems? I'm also looking for some sort of manual or user guide to the SEM, hopefully in pdf form, if anyone is willing to share.
Thank you for your time,
Andrew Ornelas Metallurgical Lab Technician Intertek APTECH 601 West California Avenue Sunnyvale, CA 94086
This email may contain confidential or privileged information, if you are not the intended recipient, or the person responsible for delivering the message to the intended recipient then please notify us by return email immediately. Should you have received this email in error then you should not copy this for any purpose nor disclose its contents to any other person.
http://www.intertek.com
==============================Original Headers============================== 13, 35 -- From andrew.ornelas-at-intertek.com Mon Jan 23 17:53:43 2012 13, 35 -- Received: from gateout02.mbox.net (gateout02.mbox.net [165.212.64.22]) 13, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NNrhhA030068 13, 35 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 17:53:43 -0600 13, 35 -- Received: from gateout02.mbox.net (gwo2-lo [127.0.0.1]) 13, 35 -- by gateout02.mbox.net (Postfix) with ESMTP id 363E84BC062 13, 35 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 23:53:43 +0000 (GMT) 13, 35 -- X-USANET-Received: from gateout02.mbox.net [127.0.0.1] by gateout02.mbox.net via mtad (C8.MAIN.3.72B) 13, 35 -- with ESMTP id 328qawX2M8880Mo2; Mon, 23 Jan 2012 23:53:38 -0000 13, 35 -- X-USANET-Routed: 6 gwsout-disclaimer Q:watd 13, 35 -- X-USANET-Routed: 10 gwsout-externalarchive C:gwsarchive:625 intertek.com.gw.7754-at-incoming02.seccas.com 13, 35 -- X-USANET-Routed: 3 gwsout-vs Q:bmvirus 13, 35 -- Received: from GW2.EXCHPROD.USA.NET [165.212.116.254] by gateout02.mbox.net via smtad (C8.MAIN.3.72B) 13, 35 -- with ESMTP id XID540qawX2M7134Xo2; Mon, 23 Jan 2012 23:53:38 -0000 13, 35 -- X-USANET-Source: 165.212.116.254 IN andrew.ornelas-at-intertek.com GW2.EXCHPROD.USA.NET 13, 35 -- X-USANET-MsgId: XID540qawX2M7134Xo2 13, 35 -- Received: from VS4.EXCHPROD.USA.NET ([10.116.208.142]) by GW2.EXCHPROD.USA.NET with Microsoft SMTPSVC(6.0.3790.4675); 13, 35 -- Mon, 23 Jan 2012 16:53:37 -0700 13, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 13, 35 -- Content-class: urn:content-classes:message 13, 35 -- MIME-Version: 1.0 13, 35 -- Content-Type: text/plain; 13, 35 -- charset="iso-8859-1" 13, 35 -- Subject: Leo 1550 help 13, 35 -- Date: Mon, 23 Jan 2012 16:53:36 -0700 13, 35 -- Message-ID: {9CB942F41207654AA36C24134BF802A20813FB-at-VS4.EXCHPROD.USA.NET} 13, 35 -- X-MS-Has-Attach: 13, 35 -- X-MS-TNEF-Correlator: 13, 35 -- Thread-Topic: Leo 1550 help 13, 35 -- Thread-Index: AczaKjiYzomdThTSRGOkCIMjBn0LcA== 13, 35 -- From: "Andrew Ornelas Intertek" {andrew.ornelas-at-intertek.com} 13, 35 -- To: {Microscopy-at-microscopy.com} 13, 35 -- X-OriginalArrivalTime: 23 Jan 2012 23:53:37.0995 (UTC) FILETIME=[39CA69B0:01CCDA2A] 13, 35 -- Content-Transfer-Encoding: 8bit 13, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0NNrhhA030068 ==============================End of - Headers==============================
Is "The Man In The White Suit" the earliest scene with an EM in movies that anyone knows of?
Great movie by the way. john
At 02:01 PM 1/23/2012, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 24 -- From donovan-at-uoregon.edu Mon Jan 23 17:57:23 2012 7, 24 -- Received: from smtp.uoregon.edu (cc-mserv2.uoregon.edu [128.223.142.127]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NNvNjd004440 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 17:57:23 -0600 7, 24 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 7, 24 -- (authenticated bits=0) 7, 24 -- by smtp.uoregon.edu (8.14.5/8.14.5) with ESMTP id q0NNvMdI022487 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 15:57:22 -0800 7, 24 -- Message-Id: {201201232357.q0NNvMdI022487-at-smtp.uoregon.edu} 7, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 24 -- Date: Mon, 23 Jan 2012 15:56:55 -0800 7, 24 -- To: microscopy-at-microscopy.com 7, 24 -- From: John Donovan {donovan-at-uoregon.edu} 7, 24 -- Subject: Re: [Microscopy] EMs in the Movies 7, 24 -- In-Reply-To: {201201232201.q0NM1nes016684-at-ns.microscopy.com} 7, 24 -- References: {201201232201.q0NM1nes016684-at-ns.microscopy.com} 7, 24 -- Mime-Version: 1.0 7, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 24 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.6.7361,1.0.211,0.0.0000 7, 24 -- definitions=2012-01-23_05:2012-01-23,2012-01-23,1970-01-01 signatures=0 7, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=48 7, 24 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=-45 7, 24 -- reason=mlx scancount=1 engine=6.0.2-1012030000 definitions=main-1201230279 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both amy.albin-at-utoledo.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: amy.albin-at-utoledo.edu Name: Amy Albin
Organization: University of Toledo
Title-Subject: [Filtered] One more manual...
Message: Everyone was so helpful when I was searching for a manual last time, I thought I might try my luck again! Now I am looking for a MT6000-XL RMC ultramicrotome manual, and Google searching isnt giving me any good leads. I emailed RMC through their website, but havent yet heard a response. Thank you all for your help! -Amy
I agree with everything Warren says, the average EDS/EDX system is not set up for boron analysis out of the box. You need to set it up yourself, and then test with known reference materials.
I believe the best way is with WDS EPMA (probe) analysis. Most probes have special diffracting crystals optimised for the boron x-ray.
cheers, Ron ________________________________________ X-from: wesaia-at-iastate.edu [wesaia-at-iastate.edu] Sent: Tuesday, 24 January 2012 3:20 AM To: Ron Rasch
The first thing you do is proceed with great caution. If your system can detect boron, I am sure it will spit out a number. It might even have some resemblance to the truth, but probably not.
The second thing you should probably do is start looking into the difficulty of light element analysis. You have boron and oxygen (light elements) combined with zinc (a rather heavy element). Look into the ZAF factors and see how big the absorption corrections. Ask yourself how well you know the mass absorption coefficients for those combinations. See what you can do about changing conditions (dropping voltage) to reduce the magnitude of the corrections.
The third thing you do would be to make sure your system can see the boron peak. My guess is that you are probably not optimized to detect boron. We had an old Kevex Quantum detector running on one of the early IXRF systems. You might be able to adjust your discriminators to better see the peak. We had to. In the end, we could see most of the boron peak, but not much if any of the background on the low energy side.
The fourth thing would be to get some reference materials. If your system cannot give you right answers on a known compound (and it probably won't), then you can forget about trying to analyze an unknown compound. You may be able to collect your own standards to help the matter, but that is not a job for novices. (If you succeed, you may have progressed beyond the novice stage. There is much more to be said on this topic.)
Another thing is to consider the limitations of EDS in general. I suppose they will not have a flat, polished sample. You'll need to consider the effects of geometry. They affect results and become more significant the lighter the elements. Depending on where you hit a particle (on the side near the detector or the side away), you can probably produce any results you want.
Finally, I would get a large supply of salt so you can hand out your results with many grains. Seriously, I would try to get your client to accept qualitative results such as "The sample shows Zn, B, and O. It could be borate." This will be even more convincing if you can compare spectra of their unknown to a known sample of zinc borate and say, "See, they match". That may be as far as you really want to push it.
Regards, Warren Straszheim
-----Original Message----- X-from: donk-at-ardl.com [mailto:donk-at-ardl.com] Sent: Monday, January 23, 2012 10:42 AM To: wesaia-at-iastate.edu
"The Andromeda Strain (1971) featured a "Forgflo" TEM (formerly RCA EMU-3). Forgflo Corp. took over the RCA EM business around 1970, just in time to get the Forgflo name on a TEM in that movie. The movie was great, but of course the depiction of the use of the TEM was pretty ridiculous. Curiously, a few years ago I was chatting with a woman on a flight out of Tokyo Narita airport, while we were getting settled in the business cabin. When I mentioned working with electron microscopes, she said that her father, a long time ago, owned a company that for a short while sold EMs. She was amazed when I said "Oh, you mean Forgflo...the microscope in The Andromeda Strain"... :-). I've never personally seen a microscope with the Forgflo logo on it...I wonder if anyone on the listserver has any familiarity with the name...?
Also, "Avatar" has a giant machine in the early part of the movie that I believe is supposed to be an electron microscope...check it out.
Larry
Dr. Lawrence F. Allard, FMSA Distinguished Research Staff Member High Temperature Materials Laboratory Microscopy Group Materials Science and Technology Division Oak Ridge National Laboratory 1 Bethel Valley Road PO Box 2008 Oak Ridge, TN 37831-6064 (note: the last 4 lines are sufficient for mailing or overnight courier service) 865-607-1144 (cell) 865-576-5413 (fax) allardLFjr-at-ornl.gov
-----Original Message----- X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu] Sent: Monday, January 23, 2012 6:58 PM To: Allard Jr, Lawrence Frederick
Is "The Man In The White Suit" the earliest scene with an EM in movies that anyone knows of?
Great movie by the way. john
At 02:01 PM 1/23/2012, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 24 -- From donovan-at-uoregon.edu Mon Jan 23 17:57:23 2012 7, 24 -- Received: from smtp.uoregon.edu (cc-mserv2.uoregon.edu [128.223.142.127]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NNvNjd004440 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 17:57:23 -0600 7, 24 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 7, 24 -- (authenticated bits=0) 7, 24 -- by smtp.uoregon.edu (8.14.5/8.14.5) with ESMTP id q0NNvMdI022487 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 15:57:22 -0800 7, 24 -- Message-Id: {201201232357.q0NNvMdI022487-at-smtp.uoregon.edu} 7, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 24 -- Date: Mon, 23 Jan 2012 15:56:55 -0800 7, 24 -- To: microscopy-at-microscopy.com 7, 24 -- From: John Donovan {donovan-at-uoregon.edu} 7, 24 -- Subject: Re: [Microscopy] EMs in the Movies 7, 24 -- In-Reply-To: {201201232201.q0NM1nes016684-at-ns.microscopy.com} 7, 24 -- References: {201201232201.q0NM1nes016684-at-ns.microscopy.com} 7, 24 -- Mime-Version: 1.0 7, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 24 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.6.7361,1.0.211,0.0.0000 7, 24 -- definitions=2012-01-23_05:2012-01-23,2012-01-23,1970-01-01 signatures=0 7, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=48 7, 24 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=-45 7, 24 -- reason=mlx scancount=1 engine=6.0.2-1012030000 definitions=main-1201230279 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 30 -- From allardlfjr-at-ornl.gov Mon Jan 23 21:47:35 2012 17, 30 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.14.62]) 17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0O3lZFv008660 17, 30 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 21:47:35 -0600 17, 30 -- X-SG: RELAYLIST 17, 30 -- X-IronPort-AV: E=Sophos;i="4.71,560,1320642000"; 17, 30 -- d="scan'208";a="5367555" 17, 30 -- Received: from ironport-7603-src.ens.ornl.gov (HELO exchhub2.ornl.gov) ([10.1.86.100]) 17, 30 -- by iron2.ornl.gov with ESMTP/TLS/RC4-MD5; 23 Jan 2012 22:47:34 -0500 17, 30 -- Received: from EXCHMBB.ornl.gov ([192.168.3.201]) by exchhub2.ornl.gov 17, 30 -- ([160.91.2.112]) with mapi; Mon, 23 Jan 2012 22:47:35 -0500 17, 30 -- From: "Allard Jr, Lawrence Frederick" {allardlfjr-at-ornl.gov} 17, 30 -- To: "'donovan-at-uoregon.edu'" {donovan-at-uoregon.edu} , 17, 30 -- "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 17, 30 -- Date: Mon, 23 Jan 2012 22:47:34 -0500 17, 30 -- Subject: RE: [Microscopy] Re: EMs in the Movies 17, 30 -- Thread-Topic: [Microscopy] Re: EMs in the Movies 17, 30 -- Thread-Index: AczaKtvDsddVkLzBTSmxmtMNnJbLDQAHe0MA 17, 30 -- Message-ID: {8191D79CA31A4C49A73FC6191BAFC517745839E73F-at-EXCHMBB.ornl.gov} 17, 30 -- References: {201201232358.q0NNw7vc007009-at-ns.microscopy.com} 17, 30 -- In-Reply-To: {201201232358.q0NNw7vc007009-at-ns.microscopy.com} 17, 30 -- Accept-Language: en-US 17, 30 -- Content-Language: en-US 17, 30 -- X-MS-Has-Attach: 17, 30 -- X-MS-TNEF-Correlator: 17, 30 -- acceptlanguage: en-US 17, 30 -- Content-Type: text/plain; charset="us-ascii" 17, 30 -- MIME-Version: 1.0 17, 30 -- Content-Transfer-Encoding: 8bit 17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0O3lZFv008660 ==============================End of - Headers==============================
this just to try to figure out whether you would like to get not only references but (if you like) some .pdf on the making and the method used for staining.
Interesting method, but not the least for a substitution of uranyl-acetate (see below)
e.g. Abstract 1993: 27-V-1030 Enhancement of the BSE signal from hydrous SEM samples by use of a platinum blue. Keiichi Tanaka and Kenji Inagaki Seirei Christopher Coll. Nurs. ,Hamamatsu, 433 Japan. The most serious problem for observing hydrous biological samples is deterioration of signal-noise ratio in consequence of lowering of vacuum in specimen chambers. Although metal coating is usually used for increasing SE and BSE yields on ordinary SEM studies, the method cannot be applied for wet samples. Instead of the metal coating method, therefore, we newly devised a staining method with a 'platinum blue'. Platinum blue is a general term of polymeric compounds of deep blue in which platinum [is?] coordinated to amide groups. In this study, we used a platinum blue prepared from the reaction of cis-dichlorodiammineplatinum (II) with thymidine, being expected to have a molecular formula [Pt4 (NHx)x . (CxHxxOx) 4] +x.
{Note added by W. Muss: unfortunately the term [Pt4 (NHx)x . (CxHxxOx) 4] +x is printed so small (poor quality) that identification of Atom-numbers is not possible from this specific reprint, but from the paper out of 2007 - see below - it is ([Pt4(NH3)8(C6H13O5)4]^+5) pH = 3-4. }
This compound did not crystallize and the Pt particles were not recognized on specimen surfaces at the observation of 100.000X. The staining method was as follows. The specimens were dipped in the concentrated solution for about 15-30 min and rinsed in distilled water. After removing excess fluid, they are immediately observed in a SEM for wet samples. This method was very effective for enhancing SE and BSE yields, consequently, we could get enough signals for obtaining specimen images even at the vacuum of a 4.0 Torr.
or: Paper from 2007 "Paper introduces an aqueous solution of Platinum Blue (Pt-Blue) as an alternative to Uranyl actetate (UA) for staining in Transmission EM.....
There is also a paper from 2009..
On 7th of Dec. 2011 I posted the following in TOPICS/ResearchGate (==} { http://www.researchgate.net/topic/Electron_Microscopy/ } ) or - better, since directly -
Header: (TEM): Substitute reagents for Uranyl-Acetate in positive and negative staining of resin ultrathin sections.
{Dear Colleagues, I would like to inform you of an article which recently was published in Journal of Electron Microscopy (Toyko) which I found to be perhaps of interest also to you Electron Microscopists:
Masamichi Nakakoshi, Hideo Nishioka, and Eisaku Katayama New versatile staining reagents for biological transmission electron microscopy that substitute for uranyl acetate}
Just only citing/referencing......not yet working with Platinum Blue, but trying to play with the Lanthanides Samarium- & or Gadolinium-Triacetate-opportunity
Please tell me, whether you will accept sending of pdf's to your mail account or not...
Best regards,
Wolfgang MUSS Member of MSA SALZBURG, Austria
} -----Ursprüngliche Nachricht----- } Von: bryan.todd.hansen-at-gmail.com [mailto:bryan.todd.hansen-at-gmail.com] } Gesendet: Montag, 23. Jänner 2012 22:29 } An: Muß Wolfgang } Betreff: [Microscopy] Platinum Blue Recipe } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All, } } I've found a couple of papers that list Pt-blue as an alternative to } Uranyl } Acetate, but I haven't been able to track down the way to make it. } Â Does } anyone have a working recipe as how to make the Pt-blue? Â Thanks for } any } help/tips. } } -- } Bryan Hansen } EM Technician } Rocky Mountain Laboratory } NIAID/NIH } bryan.todd.hansen-at-gmail.com } hansenbry-at-niaid.nih.gov } 406-363-9202 } } } ==============================Original Headers============================== } 4, 26 -- From bryan.todd.hansen-at-gmail.com Mon Jan 23 15:26:48 2012 } 4, 26 -- Received: from mail-gx0-f169.google.com (mail-gx0- } f169.google.com [209.85.161.169]) } 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id q0NLQmIG025346 } 4, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 23 Jan 2012 15:26:48 } -0600 } 4, 26 -- Received: by ggnk5 with SMTP id k5so1810825ggn.0 } 4, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 23 Jan 2012 } 13:26:48 -0800 (PST) } 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 26 -- d=gmail.com; s=gamma; } 4, 26 -- h=mime-version:date:message- } id:subject:from:to:content-type } 4, 26 -- :content-transfer-encoding; } 4, 26 -- bh=yqrcR2SWNEedsdIRyGKovxki6W8TM/HNAq7c+ISLP5c=; } 4, 26 -- } b=aDers0mrG8rloIEXPJtgY6jEuKuHpjlG4qYGCdJHJBaZBy2Mr3UT8PexTCSLCwoQ0s } 4, 26 -- } l5UER75KTIz5bM6VLGdz/jlHqMoIL9Gp1cko5tFHDzbEWGqljzXOpbhZKMabo8agbMjg } 4, 26 -- TxaGsXHORnSpf1E5k1PASV4S26Svd5Jbr0H2k= } 4, 26 -- MIME-Version: 1.0 } 4, 26 -- Received: by 10.50.195.227 with SMTP id } ih3mr11722639igc.19.1327354008070; } 4, 26 -- Mon, 23 Jan 2012 13:26:48 -0800 (PST) } 4, 26 -- Received: by 10.231.96.79 with HTTP; Mon, 23 Jan 2012 13:26:48 } -0800 (PST) } 4, 26 -- Date: Mon, 23 Jan 2012 14:26:48 -0700 } 4, 26 -- Message-ID: {CALkYmiELSEwCrS_dPGoG4RbBtzP84nk_eRWG- } 49weG+Na02GGQ-at-mail.gmail.com} } 4, 26 -- Subject: Platinum Blue Recipe } 4, 26 -- From: Bryan Hansen {bryan.todd.hansen-at-gmail.com} } 4, 26 -- To: Microscopy List Server {Microscopy-at-Microscopy.Com} } 4, 26 -- Content-Type: text/plain; charset=UTF-8 } 4, 26 -- Content-Transfer-Encoding: 8bit } 4, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id q0NLQmIG025346 } ==============================End of - Headers==============================
==============================Original Headers============================== 22, 40 -- From W.Muss-at-salk.at Tue Jan 24 03:16:37 2012 22, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 22, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0O9GacE029744 22, 40 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 03:16:36 -0600 22, 40 -- Received: from localhost (localhost [127.0.0.1]) 22, 40 -- by hermes.salk.at (Postfix) with ESMTP id 1DE6FC38A3 22, 40 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 10:16:35 +0100 (CET) 22, 40 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 22, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 22, 40 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 22, 40 -- with ESMTP id YZrA3K5k0uz2 for {microscopy-at-microscopy.com} ; 22, 40 -- Tue, 24 Jan 2012 10:16:34 +0100 (CET) 22, 40 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 22, 40 -- by hermes.salk.at (Postfix) with ESMTP id 627E6C389E 22, 40 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 10:16:34 +0100 (CET) 22, 40 -- Received: from n2rz123.lksdom21.lks.local ([192.168.101.123]) 22, 40 -- by N2EX199.lks.local with ESMTP; 24 Jan 2012 10:17:42 +0100 22, 40 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.133]) by n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.4675); 22, 40 -- Tue, 24 Jan 2012 10:16:31 +0100 22, 40 -- Content-class: urn:content-classes:message 22, 40 -- MIME-Version: 1.0 22, 40 -- Content-Type: text/plain; 22, 40 -- charset="iso-8859-1" 22, 40 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 40 -- Subject: Re: [Microscopy] Platinum Blue Recipe 22, 40 -- Date: Tue, 24 Jan 2012 10:16:29 +0100 22, 40 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062F33584-at-N1RZ116.lksdom21.lks.local} 22, 40 -- In-Reply-To: {201201232129.q0NLT7Wl030093-at-ns.microscopy.com} 22, 40 -- X-MS-Has-Attach: 22, 40 -- X-MS-TNEF-Correlator: 22, 40 -- Thread-Topic: Re: [Microscopy] Platinum Blue Recipe 22, 40 -- Thread-Index: AczaFgyuU7lDNLtETNSP57P89h+FPgAXvLQw 22, 40 -- References: {201201232129.q0NLT7Wl030093-at-ns.microscopy.com} 22, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 22, 40 -- To: {microscopy-at-microscopy.com} 22, 40 -- Cc: {tobryan.todd.hansen-at-gmail.com} 22, 40 -- X-OriginalArrivalTime: 24 Jan 2012 09:16:31.0197 (UTC) FILETIME=[DC3010D0:01CCDA78] 22, 40 -- X-Scanned-By: SALK-Content-Filter 22, 40 -- Content-Transfer-Encoding: 8bit 22, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0O9GacE029744 ==============================End of - Headers==============================
I was watching the second episode of the first season of the english show sherlock last night, Sherlock used a Leica dissecting microscope to get some lovely scanning EM images of pollen which were characterized as a pathogenŠ
Simon
Simon C. Watkins PhD Professor, Cell Biology Professor, Immunology Vice Chair Cell Biology Director Center for Biologic Imaging University of Pittsburgh BSTS 225
3500 Terrace St Pittsburgh PA 15261 412-352-2277 Www.cbi.pitt.edu
On 1/23/12 10:51 PM, "allardlfjr-at-ornl.gov" {allardlfjr-at-ornl.gov} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 15, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 24 07:51:01 2012 15, 25 -- Received: from znl.com ([206.69.208.20]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0ODp1dq021802 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 07:51:01 -0600 15, 25 -- Received: from localhost (localhost [127.0.0.1]) 15, 25 -- by znl.com (Postfix) with ESMTP id 0DC1547CBA5 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 07:51:01 -0600 (CST) 15, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 25 -- Received: from znl.com ([127.0.0.1]) 15, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 25 -- with ESMTP id rcTov40h95n5 for {microscopy-at-microscopy.com} ; 15, 25 -- Tue, 24 Jan 2012 07:50:47 -0600 (CST) 15, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 15, 25 -- by znl.com (Postfix) with ESMTPA id B2BDF47CB94 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 07:50:47 -0600 (CST) 15, 25 -- Message-ID: {4F1EB737.2010207-at-microscopy.com} 15, 25 -- Date: Tue, 24 Jan 2012 07:50:47 -0600 15, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 25 -- Reply-To: "Watkins, Simon C" {swatkins-at-pitt.edu} 15, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 15, 25 -- MIME-Version: 1.0 15, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 25 -- Subject: [Microscopy] EMs in the Movies 15, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
The Forgflo EM in The Andromeda Strain was a 4C model. Just as I was finishing my Master's at Cal State Long Beach the Biology dept. bought one. Our service engineer was the one who set up the EM for the movie.
Geoff
On 1/23/2012 10:48 PM, allardlfjr-at-ornl.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } "The Andromeda Strain (1971) featured a "Forgflo" TEM (formerly RCA EMU-3). Forgflo Corp. took over the RCA EM business around 1970, just in time to get the Forgflo name on a TEM in that movie. The movie was great, but of course the depiction of the use of the TEM was pretty ridiculous. Curiously, a few years ago I was chatting with a woman on a flight out of Tokyo Narita airport, while we were getting settled in the business cabin. When I mentioned working with electron microscopes, she said that her father, a long time ago, owned a company that for a short while sold EMs. She was amazed when I said "Oh, you mean Forgflo...the microscope in The Andromeda Strain"... :-). I've never personally seen a microscope with the Forgflo logo on it...I wonder if anyone on the listserver has any familiarity with the name...? } } Also, "Avatar" has a giant machine in the early part of the movie that I believe is supposed to be an electron microscope...check it out. } } Larry } } Dr. Lawrence F. Allard, FMSA } Distinguished Research Staff Member } High Temperature Materials Laboratory } Microscopy Group } Materials Science and Technology Division } Oak Ridge National Laboratory } 1 Bethel Valley Road } PO Box 2008 } Oak Ridge, TN 37831-6064 } (note: the last 4 lines are sufficient for mailing or overnight courier service) } 865-607-1144 (cell) } 865-576-5413 (fax) } allardLFjr-at-ornl.gov } } -----Original Message----- } X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu] } Sent: Monday, January 23, 2012 6:58 PM } To: Allard Jr, Lawrence Frederick } Subject: [Microscopy] Re: EMs in the Movies } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Is "The Man In The White Suit" the earliest scene with an EM in } movies that anyone knows of? } } Great movie by the way. } john } } At 02:01 PM 1/23/2012, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi Listers } } } } I have for a long time been keen to find references to EMs in movies and } } put them on a website for all to see. Every time I think about it I } } promptly forget and the cycle repeats not this time! } } } } I wondered is anyone has spotted EMs in movies/tv shows/documentaries? } } If so what and where? If you can point out the make, model and any } } inaccuracies all the better! } } } } So far the ones I can remember are: } } } } 1. Flash Gordon 1936 (Controls of the ship are a TEM) } } 2. Independence Day in Area 51 scene } } 3. The Man in White Suit } } 4. Predator 2 } } 5. CSI had a Hitachi S3400 once I recall } } 6. There was a fake one in Spiderman 2 } } 7. Blade Runner } } } } Any more would be greatly appreciated including those not in English! } } } } Regards } } John } } } } } } . } } } } ==============================Original Headers============================== } } 9, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 15:59:16 2012 } } 9, 29 -- Received: from mail-ww0-f49.google.com } } (mail-ww0-f49.google.com [74.125.82.49]) } } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id q0NLxGtL010271 } } 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 } } 15:59:16 -0600 } } 9, 29 -- Received: by wgbds1 with SMTP id ds1so1372983wgb.18 } } 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 } } 13:59:15 -0800 (PST) } } 9, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 9, 29 -- d=gmail.com; s=gamma; } } 9, 29 -- h=message-id:date:from:user-agent:mime-version:to:subject } } 9, 29 -- :content-type:content-transfer-encoding; } } 9, 29 -- bh=Ba4Sdz+tb/IFyn9mxcOj+s3UNCp0MilqOz/HQ13Ssj8=; } } 9, 29 } } -- b=L8Ld1beKC4vs5R3bY4RCUhI0X0RMXmT2MuJuH1/WpJTViudtsP70vIPIVirnCob/sc } } 9, 29 } } -- } } Rbnz27D1oogAIyvhuRWv/ADcZNe/CJg8vsMPlRBcqgTZJnne4dy/xSrZAmTNjqXWAp2f } } 9, 29 -- UxwFAe6RdM4JSInulGqbxS63niVp0N/bhiVuc= } } 9, 29 -- Received: by 10.180.95.1 with SMTP id } } dg1mr8530030wib.21.1327355955629; } } 9, 29 -- Mon, 23 Jan 2012 13:59:15 -0800 (PST) } } 9, 29 -- Received: from [127.0.0.1] ([87.114.247.49]) } } 9, 29 -- by mx.google.com with ESMTPS id } } n3sm45503712wiz.9.2012.01.23.13.59.14 } } 9, 29 -- (version=TLSv1/SSLv3 cipher=OTHER); } } 9, 29 -- Mon, 23 Jan 2012 13:59:14 -0800 (PST) } } 9, 29 -- Message-ID: {4F1DD832.1070806-at-gmail.com} } } 9, 29 -- Date: Mon, 23 Jan 2012 21:59:14 +0000 } } 9, 29 -- From: John Mitchels {john.mitchels-at-gmail.com} } } 9, 29 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:9.0) } } Gecko/20111222 Thunderbird/9.0.1 } } 9, 29 -- MIME-Version: 1.0 } } 9, 29 -- To: Microscopy-at-microscopy.com } } 9, 29 -- Subject: EMs in the Movies } } 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 9, 29 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 7, 24 -- From donovan-at-uoregon.edu Mon Jan 23 17:57:23 2012 } 7, 24 -- Received: from smtp.uoregon.edu (cc-mserv2.uoregon.edu [128.223.142.127]) } 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NNvNjd004440 } 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 17:57:23 -0600 } 7, 24 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) } 7, 24 -- (authenticated bits=0) } 7, 24 -- by smtp.uoregon.edu (8.14.5/8.14.5) with ESMTP id q0NNvMdI022487 } 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 15:57:22 -0800 } 7, 24 -- Message-Id: {201201232357.q0NNvMdI022487-at-smtp.uoregon.edu} } 7, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 7, 24 -- Date: Mon, 23 Jan 2012 15:56:55 -0800 } 7, 24 -- To: microscopy-at-microscopy.com } 7, 24 -- From: John Donovan {donovan-at-uoregon.edu} } 7, 24 -- Subject: Re: [Microscopy] EMs in the Movies } 7, 24 -- In-Reply-To: {201201232201.q0NM1nes016684-at-ns.microscopy.com} } 7, 24 -- References: {201201232201.q0NM1nes016684-at-ns.microscopy.com} } 7, 24 -- Mime-Version: 1.0 } 7, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 7, 24 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.6.7361,1.0.211,0.0.0000 } 7, 24 -- definitions=2012-01-23_05:2012-01-23,2012-01-23,1970-01-01 signatures=0 } 7, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=48 } 7, 24 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=-45 } 7, 24 -- reason=mlx scancount=1 engine=6.0.2-1012030000 definitions=main-1201230279 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 17, 30 -- From allardlfjr-at-ornl.gov Mon Jan 23 21:47:35 2012 } 17, 30 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.14.62]) } 17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0O3lZFv008660 } 17, 30 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 21:47:35 -0600 } 17, 30 -- X-SG: RELAYLIST } 17, 30 -- X-IronPort-AV: E=Sophos;i="4.71,560,1320642000"; } 17, 30 -- d="scan'208";a="5367555" } 17, 30 -- Received: from ironport-7603-src.ens.ornl.gov (HELO exchhub2.ornl.gov) ([10.1.86.100]) } 17, 30 -- by iron2.ornl.gov with ESMTP/TLS/RC4-MD5; 23 Jan 2012 22:47:34 -0500 } 17, 30 -- Received: from EXCHMBB.ornl.gov ([192.168.3.201]) by exchhub2.ornl.gov } 17, 30 -- ([160.91.2.112]) with mapi; Mon, 23 Jan 2012 22:47:35 -0500 } 17, 30 -- From: "Allard Jr, Lawrence Frederick" {allardlfjr-at-ornl.gov} } 17, 30 -- To: "'donovan-at-uoregon.edu'" {donovan-at-uoregon.edu} , } 17, 30 -- "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } 17, 30 -- Date: Mon, 23 Jan 2012 22:47:34 -0500 } 17, 30 -- Subject: RE: [Microscopy] Re: EMs in the Movies } 17, 30 -- Thread-Topic: [Microscopy] Re: EMs in the Movies } 17, 30 -- Thread-Index: AczaKtvDsddVkLzBTSmxmtMNnJbLDQAHe0MA } 17, 30 -- Message-ID: {8191D79CA31A4C49A73FC6191BAFC517745839E73F-at-EXCHMBB.ornl.gov} } 17, 30 -- References: {201201232358.q0NNw7vc007009-at-ns.microscopy.com} } 17, 30 -- In-Reply-To: {201201232358.q0NNw7vc007009-at-ns.microscopy.com} } 17, 30 -- Accept-Language: en-US } 17, 30 -- Content-Language: en-US } 17, 30 -- X-MS-Has-Attach: } 17, 30 -- X-MS-TNEF-Correlator: } 17, 30 -- acceptlanguage: en-US } 17, 30 -- Content-Type: text/plain; charset="us-ascii" } 17, 30 -- MIME-Version: 1.0 } 17, 30 -- Content-Transfer-Encoding: 8bit } 17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0O3lZFv008660 } ==============================End of - Headers============================== } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
Don't know how this topic came up, but of course Blade Runner has a scene featuring a behind-the counter SEM with no need for sample prep, used to quickly distinguish a scale of a replicant-snake from a replicant-fish. Another scene features an image-enhancing device that might in retrospect be seen as a form of light-field imaging.
Peter
==============================Original Headers============================== 2, 19 -- From germpore-at-sonic.net Tue Jan 24 18:39:00 2012 2, 19 -- Received: from b.mail.sonic.net (b.mail.sonic.net [64.142.19.5]) 2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0P0cxHT004703 2, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 18:39:00 -0600 2, 19 -- Received: from 209-148-96-171.dial.dynamic.sonic.net (209-148-96-171.dial.dynamic.sonic.net [209.148.96.171]) 2, 19 -- (authenticated bits=0) 2, 19 -- by b.mail.sonic.net (8.13.8.Beta0-Sonic/8.13.7) with ESMTP id q0P0cvUN018770 2, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 16:38:58 -0800 2, 19 -- Message-Id: {BCE76AFA-12D3-4CE1-9507-95903B749A82-at-sonic.net} 2, 19 -- From: Peter Werner {germpore-at-sonic.net} 2, 19 -- To: Microscopy-at-microscopy.com 2, 19 -- In-Reply-To: {201201240355.q0O3t2WA022366-at-ns.microscopy.com} 2, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 19 -- Content-Transfer-Encoding: 7bit 2, 19 -- Mime-Version: 1.0 (Apple Message framework v936) 2, 19 -- Subject: Re: [Microscopy] EMs in the Movies 2, 19 -- Date: Tue, 24 Jan 2012 16:38:57 -0800 2, 19 -- References: {201201240355.q0O3t2WA022366-at-ns.microscopy.com} 2, 19 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
You can watch the BBC on iPlayer if you can get a proxy server that makes it look like you are in the UK. We just watched the second series of Sherlock.
Sent from my iPad. -- John Mansfield PhD Cphys MInstP 2010 Microscopy & Microanalysis Program Chair North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
On Jan 24, 2012, at 9:00 AM, microscopylistserver-noreply-at-microscopy.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was watching the second episode of the first season of the english show } sherlock last night, Sherlock used a Leica dissecting microscope to get } some lovely scanning EM images of pollen which were characterized as a } pathogen� } } Simon } } Simon C. Watkins PhD } Professor, Cell Biology } Professor, Immunology } Vice Chair Cell Biology } Director Center for Biologic Imaging } University of Pittsburgh } BSTS 225 } } 3500 Terrace St } Pittsburgh PA 15261 } 412-352-2277 } Www.cbi.pitt.edu } } } } } } } On 1/23/12 10:51 PM, "allardlfjr-at-ornl.gov" {allardlfjr-at-ornl.gov} wrote: } } } } } } } } } -------------------------------------------------------------------------- } } -- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------------------- } } -- } } } } "The Andromeda Strain (1971) featured a "Forgflo" TEM (formerly RCA } } EMU-3). Forgflo Corp. took over the RCA EM business around 1970, just in } } time to get the Forgflo name on a TEM in that movie. The movie was } } great, but of course the depiction of the use of the TEM was pretty } } ridiculous. Curiously, a few years ago I was chatting with a woman on a } } flight out of Tokyo Narita airport, while we were getting settled in the } } business cabin. When I mentioned working with electron microscopes, she } } said that her father, a long time ago, owned a company that for a short } } while sold EMs. She was amazed when I said "Oh, you mean Forgflo...the } } microscope in The Andromeda Strain"... :-). I've never personally seen a } } microscope with the Forgflo logo on it...I wonder if anyone on the } } listserver has any familiarity with the name...? } } } } Also, "Avatar" has a giant machine in the early part of the movie that I } } believe is supposed to be an electron microscope...check it out. } } } } Larry } } } } Dr. Lawrence F. Allard, FMSA } } Distinguished Research Staff Member } } High Temperature Materials Laboratory } } Microscopy Group } } Materials Science and Technology Division } } Oak Ridge National Laboratory } } 1 Bethel Valley Road } } PO Box 2008 } } Oak Ridge, TN 37831-6064 } } (note: the last 4 lines are sufficient for mailing or overnight courier } } service) } } 865-607-1144 (cell) } } 865-576-5413 (fax) } } allardLFjr-at-ornl.gov } } } } -----Original Message----- } } X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu] } } Sent: Monday, January 23, 2012 6:58 PM } } To: Allard Jr, Lawrence Frederick } } Subject: [Microscopy] Re: EMs in the Movies } } } } } } } } } } -------------------------------------------------------------------------- } } -- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------------------- } } -- } } } } Is "The Man In The White Suit" the earliest scene with an EM in } } movies that anyone knows of? } } } } Great movie by the way. } } john } } } } At 02:01 PM 1/23/2012, you wrote: } } } } } } } } } ------------------------------------------------------------------------- } } } --- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------- } } } --- } } } } } } Hi Listers } } } } } } I have for a long time been keen to find references to EMs in movies and } } } put them on a website for all to see. Every time I think about it I } } } promptly forget and the cycle repeats not this time! } } } } } } I wondered is anyone has spotted EMs in movies/tv shows/documentaries? } } } If so what and where? If you can point out the make, model and any } } } inaccuracies all the better! } } } } } } So far the ones I can remember are: } } } } } } 1. Flash Gordon 1936 (Controls of the ship are a TEM) } } } 2. Independence Day in Area 51 scene } } } 3. The Man in White Suit } } } 4. Predator 2 } } } 5. CSI had a Hitachi S3400 once I recall } } } 6. There was a fake one in Spiderman 2 } } } 7. Blade Runner } } } } } } Any more would be greatly appreciated including those not in English! } } } } } } Regards } } } John } } } } } } } } } . } } } } } } ==============================Original } } } Headers============================== } } } 9, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 15:59:16 2012 } } } 9, 29 -- Received: from mail-ww0-f49.google.com } } } (mail-ww0-f49.google.com [74.125.82.49]) } } } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id q0NLxGtL010271 } } } 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 } } } 15:59:16 -0600 } } } 9, 29 -- Received: by wgbds1 with SMTP id ds1so1372983wgb.18 } } } 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 } } } 13:59:15 -0800 (PST) } } } 9, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } } 9, 29 -- d=gmail.com; s=gamma; } } } 9, 29 -- } } } h=message-id:date:from:user-agent:mime-version:to:subject } } } 9, 29 -- :content-type:content-transfer-encoding; } } } 9, 29 -- bh=Ba4Sdz+tb/IFyn9mxcOj+s3UNCp0MilqOz/HQ13Ssj8=; } } } 9, 29 } } } -- } } } b=L8Ld1beKC4vs5R3bY4RCUhI0X0RMXmT2MuJuH1/WpJTViudtsP70vIPIVirnCob/sc } } } 9, 29 } } } -- } } } Rbnz27D1oogAIyvhuRWv/ADcZNe/CJg8vsMPlRBcqgTZJnne4dy/xSrZAmTNjqXWAp2f } } } 9, 29 -- UxwFAe6RdM4JSInulGqbxS63niVp0N/bhiVuc= } } } 9, 29 -- Received: by 10.180.95.1 with SMTP id } } } dg1mr8530030wib.21.1327355955629; } } } 9, 29 -- Mon, 23 Jan 2012 13:59:15 -0800 (PST) } } } 9, 29 -- Received: from [127.0.0.1] ([87.114.247.49]) } } } 9, 29 -- by mx.google.com with ESMTPS id } } } n3sm45503712wiz.9.2012.01.23.13.59.14 } } } 9, 29 -- (version=TLSv1/SSLv3 cipher=OTHER); } } } 9, 29 -- Mon, 23 Jan 2012 13:59:14 -0800 (PST) } } } 9, 29 -- Message-ID: {4F1DD832.1070806-at-gmail.com} } } } 9, 29 -- Date: Mon, 23 Jan 2012 21:59:14 +0000 } } } 9, 29 -- From: John Mitchels {john.mitchels-at-gmail.com} } } } 9, 29 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:9.0) } } } Gecko/20111222 Thunderbird/9.0.1 } } } 9, 29 -- MIME-Version: 1.0 } } } 9, 29 -- To: Microscopy-at-microscopy.com } } } 9, 29 -- Subject: [Filtered] EMs in the Movies } } } 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } } 9, 29 -- Content-Transfer-Encoding: 7bit } } } ==============================End of - } } } Headers============================== } } } } } } ==============================Original } } Headers============================== } } 7, 24 -- From donovan-at-uoregon.edu Mon Jan 23 17:57:23 2012 } } 7, 24 -- Received: from smtp.uoregon.edu (cc-mserv2.uoregon.edu } } [128.223.142.127]) } } 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } q0NNvNjd004440 } } 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 17:57:23 -0600 } } 7, 24 -- Received: from Probev.uoregon.edu (probev.uoregon.edu } } [128.223.10.46]) } } 7, 24 -- (authenticated bits=0) } } 7, 24 -- by smtp.uoregon.edu (8.14.5/8.14.5) with ESMTP id q0NNvMdI022487 } } 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } } verify=NOT) } } 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 15:57:22 -0800 } } 7, 24 -- Message-Id: {201201232357.q0NNvMdI022487-at-smtp.uoregon.edu} } } 7, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } } 7, 24 -- Date: Mon, 23 Jan 2012 15:56:55 -0800 } } 7, 24 -- To: microscopy-at-microscopy.com } } 7, 24 -- From: John Donovan {donovan-at-uoregon.edu} } } 7, 24 -- Subject: [Filtered] Re: [Microscopy] EMs in the Movies } } 7, 24 -- In-Reply-To: {201201232201.q0NM1nes016684-at-ns.microscopy.com} } } 7, 24 -- References: {201201232201.q0NM1nes016684-at-ns.microscopy.com} } } 7, 24 -- Mime-Version: 1.0 } } 7, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } } 7, 24 -- X-Proofpoint-Virus-Version: vendor=fsecure } } engine=2.50.10432:5.6.7361,1.0.211,0.0.0000 } } 7, 24 -- definitions=2012-01-23_05:2012-01-23,2012-01-23,1970-01-01 } } signatures=0 } } 7, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 } } spamscore=0 ipscore=0 suspectscore=48 } } 7, 24 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=-45 } } 7, 24 -- reason=mlx scancount=1 engine=6.0.2-1012030000 } } definitions=main-1201230279 } } ==============================End of - } } Headers============================== } } } } } } ==============================Original } } Headers============================== } } 17, 30 -- From allardlfjr-at-ornl.gov Mon Jan 23 21:47:35 2012 } } 17, 30 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.14.62]) } } 17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } q0O3lZFv008660 } } 17, 30 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 21:47:35 } } -0600 } } 17, 30 -- X-SG: RELAYLIST } } 17, 30 -- X-IronPort-AV: E=Sophos;i="4.71,560,1320642000"; } } 17, 30 -- d="scan'208";a="5367555" } } 17, 30 -- Received: from ironport-7603-src.ens.ornl.gov (HELO } } exchhub2.ornl.gov) ([10.1.86.100]) } } 17, 30 -- by iron2.ornl.gov with ESMTP/TLS/RC4-MD5; 23 Jan 2012 } } 22:47:34 -0500 } } 17, 30 -- Received: from EXCHMBB.ornl.gov ([192.168.3.201]) by } } exchhub2.ornl.gov } } 17, 30 -- ([160.91.2.112]) with mapi; Mon, 23 Jan 2012 22:47:35 -0500 } } 17, 30 -- From: "Allard Jr, Lawrence Frederick" {allardlfjr-at-ornl.gov} } } 17, 30 -- To: "'donovan-at-uoregon.edu'" {donovan-at-uoregon.edu} , } } 17, 30 -- "'microscopy-at-microscopy.com'" } } {microscopy-at-microscopy.com} } } 17, 30 -- Date: Mon, 23 Jan 2012 22:47:34 -0500 } } 17, 30 -- Subject: [Filtered] RE: [Microscopy] Re: EMs in the Movies } } 17, 30 -- Thread-Topic: [Microscopy] Re: EMs in the Movies } } 17, 30 -- Thread-Index: AczaKtvDsddVkLzBTSmxmtMNnJbLDQAHe0MA } } 17, 30 -- Message-ID: } } {8191D79CA31A4C49A73FC6191BAFC517745839E73F-at-EXCHMBB.ornl.gov} } } 17, 30 -- References: {201201232358.q0NNw7vc007009-at-ns.microscopy.com} } } 17, 30 -- In-Reply-To: {201201232358.q0NNw7vc007009-at-ns.microscopy.com} } } 17, 30 -- Accept-Language: en-US } } 17, 30 -- Content-Language: en-US } } 17, 30 -- X-MS-Has-Attach: } } 17, 30 -- X-MS-TNEF-Correlator: } } 17, 30 -- acceptlanguage: en-US } } 17, 30 -- Content-Type: text/plain; charset="us-ascii" } } 17, 30 -- MIME-Version: 1.0 } } 17, 30 -- Content-Transfer-Encoding: 8bit } } 17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id q0O3lZFv008660 } } ==============================End of - } } Headers============================== } } } } } } ==============================Original Headers============================== } 15, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 24 07:51:01 2012 } 15, 25 -- Received: from znl.com ([206.69.208.20]) } 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0ODp1dq021802 } 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 07:51:01 -0600 } 15, 25 -- Received: from localhost (localhost [127.0.0.1]) } 15, 25 -- by znl.com (Postfix) with ESMTP id 0DC1547CBA5 } 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 07:51:01 -0600 (CST) } 15, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 15, 25 -- Received: from znl.com ([127.0.0.1]) } 15, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 15, 25 -- with ESMTP id rcTov40h95n5 for {microscopy-at-microscopy.com} ; } 15, 25 -- Tue, 24 Jan 2012 07:50:47 -0600 (CST) } 15, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 15, 25 -- by znl.com (Postfix) with ESMTPA id B2BDF47CB94 } 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 07:50:47 -0600 (CST) } 15, 25 -- Message-ID: {4F1EB737.2010207-at-microscopy.com} } 15, 25 -- Date: Tue, 24 Jan 2012 07:50:47 -0600 } 15, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 15, 25 -- Reply-To: "Watkins, Simon C" {swatkins-at-pitt.edu} } 15, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 15, 25 -- MIME-Version: 1.0 } 15, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 25 -- Subject: [Microscopy] EMs in the Movies } 15, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } }
==============================Original Headers============================== 9, 27 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 24 19:01:39 2012 9, 27 -- Received: from znl.com ([206.69.208.20]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0P11dBi021848 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 19:01:39 -0600 9, 27 -- Received: from localhost (localhost [127.0.0.1]) 9, 27 -- by znl.com (Postfix) with ESMTP id 498A747D23A 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 19:01:39 -0600 (CST) 9, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 9, 27 -- Received: from znl.com ([127.0.0.1]) 9, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 9, 27 -- with ESMTP id X0dODsd6aOSj for {microscopy-at-microscopy.com} ; 9, 27 -- Tue, 24 Jan 2012 19:01:36 -0600 (CST) 9, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 9, 27 -- by znl.com (Postfix) with ESMTPA id 0D70747D22F 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 19:01:36 -0600 (CST) 9, 27 -- Message-ID: {4F1F546F.9000408-at-microscopy.com} 9, 27 -- Date: Tue, 24 Jan 2012 19:01:35 -0600 9, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 9, 27 -- Reply-To: John Mansfield {jfmjfm-at-umich.edu} 9, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 9, 27 -- MIME-Version: 1.0 9, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 9, 27 -- Subject: [Filtered] Re: [Microscopy] EMs in the Movies 9, 27 -- References: {201201241400.q0OE0vJn004119-at-ns.microscopy.com} 9, 27 -- In-Reply-To: {201201241400.q0OE0vJn004119-at-ns.microscopy.com} 9, 27 -- Content-Type: text/plain; charset=UTF-8; format=flowed 9, 27 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
A life science professor watched Sherlock movies. As a biological processor, he used Light microscopes a lot. So he saw something to his profession. Like me, anything related To electron microscopes, I would be very interested.
Zhen
Sent from my iPad
On Jan 24, 2012, at 7:13 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was watching the second episode of the first season of the english show } sherlock last night, Sherlock used a Leica dissecting microscope to get } some lovely scanning EM images of pollen which were characterized as a } pathogenÅ } } Simon } } Simon C. Watkins PhD } Professor, Cell Biology } Professor, Immunology } Vice Chair Cell Biology } Director Center for Biologic Imaging } University of Pittsburgh } BSTS 225 } } 3500 Terrace St } Pittsburgh PA 15261 } 412-352-2277 } Www.cbi.pitt.edu } } } } } } } On 1/23/12 10:51 PM, "allardlfjr-at-ornl.gov" {allardlfjr-at-ornl.gov} wrote: } } } } } } } } } -------------------------------------------------------------------------- } } -- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------------------- } } -- } } } } "The Andromeda Strain (1971) featured a "Forgflo" TEM (formerly RCA } } EMU-3). Forgflo Corp. took over the RCA EM business around 1970, just in } } time to get the Forgflo name on a TEM in that movie. The movie was } } great, but of course the depiction of the use of the TEM was pretty } } ridiculous. Curiously, a few years ago I was chatting with a woman on a } } flight out of Tokyo Narita airport, while we were getting settled in the } } business cabin. When I mentioned working with electron microscopes, she } } said that her father, a long time ago, owned a company that for a short } } while sold EMs. She was amazed when I said "Oh, you mean Forgflo...the } } microscope in The Andromeda Strain"... :-). I've never personally seen a } } microscope with the Forgflo logo on it...I wonder if anyone on the } } listserver has any familiarity with the name...? } } } } Also, "Avatar" has a giant machine in the early part of the movie that I } } believe is supposed to be an electron microscope...check it out. } } } } Larry } } } } Dr. Lawrence F. Allard, FMSA } } Distinguished Research Staff Member } } High Temperature Materials Laboratory } } Microscopy Group } } Materials Science and Technology Division } } Oak Ridge National Laboratory } } 1 Bethel Valley Road } } PO Box 2008 } } Oak Ridge, TN 37831-6064 } } (note: the last 4 lines are sufficient for mailing or overnight courier } } service) } } 865-607-1144 (cell) } } 865-576-5413 (fax) } } allardLFjr-at-ornl.gov } } } } -----Original Message----- } } X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu] } } Sent: Monday, January 23, 2012 6:58 PM } } To: Allard Jr, Lawrence Frederick } } Subject: [Microscopy] Re: EMs in the Movies } } } } } } } } } } -------------------------------------------------------------------------- } } -- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------------------- } } -- } } } } Is "The Man In The White Suit" the earliest scene with an EM in } } movies that anyone knows of? } } } } Great movie by the way. } } john } } } } At 02:01 PM 1/23/2012, you wrote: } } } } } } } } } ------------------------------------------------------------------------- } } } --- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------- } } } --- } } } } } } Hi Listers } } } } } } I have for a long time been keen to find references to EMs in movies and } } } put them on a website for all to see. Every time I think about it I } } } promptly forget and the cycle repeats not this time! } } } } } } I wondered is anyone has spotted EMs in movies/tv shows/documentaries? } } } If so what and where? If you can point out the make, model and any } } } inaccuracies all the better! } } } } } } So far the ones I can remember are: } } } } } } 1. Flash Gordon 1936 (Controls of the ship are a TEM) } } } 2. Independence Day in Area 51 scene } } } 3. The Man in White Suit } } } 4. Predator 2 } } } 5. CSI had a Hitachi S3400 once I recall } } } 6. There was a fake one in Spiderman 2 } } } 7. Blade Runner } } } } } } Any more would be greatly appreciated including those not in English! } } } } } } Regards } } } John } } } } } } } } } . } } } } } } ==============================Original } } } Headers============================== } } } 9, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 15:59:16 2012 } } } 9, 29 -- Received: from mail-ww0-f49.google.com } } } (mail-ww0-f49.google.com [74.125.82.49]) } } } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id q0NLxGtL010271 } } } 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 } } } 15:59:16 -0600 } } } 9, 29 -- Received: by wgbds1 with SMTP id ds1so1372983wgb.18 } } } 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 } } } 13:59:15 -0800 (PST) } } } 9, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } } 9, 29 -- d=gmail.com; s=gamma; } } } 9, 29 -- } } } h=message-id:date:from:user-agent:mime-version:to:subject } } } 9, 29 -- :content-type:content-transfer-encoding; } } } 9, 29 -- bh=Ba4Sdz+tb/IFyn9mxcOj+s3UNCp0MilqOz/HQ13Ssj8=; } } } 9, 29 } } } -- } } } b=L8Ld1beKC4vs5R3bY4RCUhI0X0RMXmT2MuJuH1/WpJTViudtsP70vIPIVirnCob/sc } } } 9, 29 } } } -- } } } Rbnz27D1oogAIyvhuRWv/ADcZNe/CJg8vsMPlRBcqgTZJnne4dy/xSrZAmTNjqXWAp2f } } } 9, 29 -- UxwFAe6RdM4JSInulGqbxS63niVp0N/bhiVuc= } } } 9, 29 -- Received: by 10.180.95.1 with SMTP id } } } dg1mr8530030wib.21.1327355955629; } } } 9, 29 -- Mon, 23 Jan 2012 13:59:15 -0800 (PST) } } } 9, 29 -- Received: from [127.0.0.1] ([87.114.247.49]) } } } 9, 29 -- by mx.google.com with ESMTPS id } } } n3sm45503712wiz.9.2012.01.23.13.59.14 } } } 9, 29 -- (version=TLSv1/SSLv3 cipher=OTHER); } } } 9, 29 -- Mon, 23 Jan 2012 13:59:14 -0800 (PST) } } } 9, 29 -- Message-ID: {4F1DD832.1070806-at-gmail.com} } } } 9, 29 -- Date: Mon, 23 Jan 2012 21:59:14 +0000 } } } 9, 29 -- From: John Mitchels {john.mitchels-at-gmail.com} } } } 9, 29 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:9.0) } } } Gecko/20111222 Thunderbird/9.0.1 } } } 9, 29 -- MIME-Version: 1.0 } } } 9, 29 -- To: Microscopy-at-microscopy.com } } } 9, 29 -- Subject: [Filtered] EMs in the Movies } } } 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } } 9, 29 -- Content-Transfer-Encoding: 7bit } } } ==============================End of - } } } Headers============================== } } } } } } ==============================Original } } Headers============================== } } 7, 24 -- From donovan-at-uoregon.edu Mon Jan 23 17:57:23 2012 } } 7, 24 -- Received: from smtp.uoregon.edu (cc-mserv2.uoregon.edu } } [128.223.142.127]) } } 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } q0NNvNjd004440 } } 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 17:57:23 -0600 } } 7, 24 -- Received: from Probev.uoregon.edu (probev.uoregon.edu } } [128.223.10.46]) } } 7, 24 -- (authenticated bits=0) } } 7, 24 -- by smtp.uoregon.edu (8.14.5/8.14.5) with ESMTP id q0NNvMdI022487 } } 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } } verify=NOT) } } 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 15:57:22 -0800 } } 7, 24 -- Message-Id: {201201232357.q0NNvMdI022487-at-smtp.uoregon.edu} } } 7, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } } 7, 24 -- Date: Mon, 23 Jan 2012 15:56:55 -0800 } } 7, 24 -- To: microscopy-at-microscopy.com } } 7, 24 -- From: John Donovan {donovan-at-uoregon.edu} } } 7, 24 -- Subject: [Filtered] Re: [Microscopy] EMs in the Movies } } 7, 24 -- In-Reply-To: {201201232201.q0NM1nes016684-at-ns.microscopy.com} } } 7, 24 -- References: {201201232201.q0NM1nes016684-at-ns.microscopy.com} } } 7, 24 -- Mime-Version: 1.0 } } 7, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } } 7, 24 -- X-Proofpoint-Virus-Version: vendor=fsecure } } engine=2.50.10432:5.6.7361,1.0.211,0.0.0000 } } 7, 24 -- definitions=2012-01-23_05:2012-01-23,2012-01-23,1970-01-01 } } signatures=0 } } 7, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 } } spamscore=0 ipscore=0 suspectscore=48 } } 7, 24 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=-45 } } 7, 24 -- reason=mlx scancount=1 engine=6.0.2-1012030000 } } definitions=main-1201230279 } } ==============================End of - } } Headers============================== } } } } } } ==============================Original } } Headers============================== } } 17, 30 -- From allardlfjr-at-ornl.gov Mon Jan 23 21:47:35 2012 } } 17, 30 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.14.62]) } } 17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } q0O3lZFv008660 } } 17, 30 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 21:47:35 } } -0600 } } 17, 30 -- X-SG: RELAYLIST } } 17, 30 -- X-IronPort-AV: E=Sophos;i="4.71,560,1320642000"; } } 17, 30 -- d="scan'208";a="5367555" } } 17, 30 -- Received: from ironport-7603-src.ens.ornl.gov (HELO } } exchhub2.ornl.gov) ([10.1.86.100]) } } 17, 30 -- by iron2.ornl.gov with ESMTP/TLS/RC4-MD5; 23 Jan 2012 } } 22:47:34 -0500 } } 17, 30 -- Received: from EXCHMBB.ornl.gov ([192.168.3.201]) by } } exchhub2.ornl.gov } } 17, 30 -- ([160.91.2.112]) with mapi; Mon, 23 Jan 2012 22:47:35 -0500 } } 17, 30 -- From: "Allard Jr, Lawrence Frederick" {allardlfjr-at-ornl.gov} } } 17, 30 -- To: "'donovan-at-uoregon.edu'" {donovan-at-uoregon.edu} , } } 17, 30 -- "'microscopy-at-microscopy.com'" } } {microscopy-at-microscopy.com} } } 17, 30 -- Date: Mon, 23 Jan 2012 22:47:34 -0500 } } 17, 30 -- Subject: [Filtered] RE: [Microscopy] Re: EMs in the Movies } } 17, 30 -- Thread-Topic: [Microscopy] Re: EMs in the Movies } } 17, 30 -- Thread-Index: AczaKtvDsddVkLzBTSmxmtMNnJbLDQAHe0MA } } 17, 30 -- Message-ID: } } {8191D79CA31A4C49A73FC6191BAFC517745839E73F-at-EXCHMBB.ornl.gov} } } 17, 30 -- References: {201201232358.q0NNw7vc007009-at-ns.microscopy.com} } } 17, 30 -- In-Reply-To: {201201232358.q0NNw7vc007009-at-ns.microscopy.com} } } 17, 30 -- Accept-Language: en-US } } 17, 30 -- Content-Language: en-US } } 17, 30 -- X-MS-Has-Attach: } } 17, 30 -- X-MS-TNEF-Correlator: } } 17, 30 -- acceptlanguage: en-US } } 17, 30 -- Content-Type: text/plain; charset="us-ascii" } } 17, 30 -- MIME-Version: 1.0 } } 17, 30 -- Content-Transfer-Encoding: 8bit } } 17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id q0O3lZFv008660 } } ==============================End of - } } Headers============================== } } } } } } ==============================Original Headers============================== } 15, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 24 07:51:01 2012 } 15, 25 -- Received: from znl.com ([206.69.208.20]) } 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0ODp1dq021802 } 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 07:51:01 -0600 } 15, 25 -- Received: from localhost (localhost [127.0.0.1]) } 15, 25 -- by znl.com (Postfix) with ESMTP id 0DC1547CBA5 } 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 07:51:01 -0600 (CST) } 15, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 15, 25 -- Received: from znl.com ([127.0.0.1]) } 15, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 15, 25 -- with ESMTP id rcTov40h95n5 for {microscopy-at-microscopy.com} ; } 15, 25 -- Tue, 24 Jan 2012 07:50:47 -0600 (CST) } 15, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 15, 25 -- by znl.com (Postfix) with ESMTPA id B2BDF47CB94 } 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 07:50:47 -0600 (CST) } 15, 25 -- Message-ID: {4F1EB737.2010207-at-microscopy.com} } 15, 25 -- Date: Tue, 24 Jan 2012 07:50:47 -0600 } 15, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 15, 25 -- Reply-To: "Watkins, Simon C" {swatkins-at-pitt.edu} } 15, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 15, 25 -- MIME-Version: 1.0 } 15, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 25 -- Subject: [Microscopy] EMs in the Movies } 15, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 27 -- From microscopylistserver-noreply-at-microscopy.com Tue Jan 24 19:02:00 2012 10, 27 -- Received: from znl.com ([206.69.208.20]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0P1206Z022645 10, 27 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 19:02:00 -0600 10, 27 -- Received: from localhost (localhost [127.0.0.1]) 10, 27 -- by znl.com (Postfix) with ESMTP id 9B14547D247 10, 27 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 19:02:00 -0600 (CST) 10, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 27 -- Received: from znl.com ([127.0.0.1]) 10, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 27 -- with ESMTP id TrsWxsgTO+cP for {microscopy-at-microscopy.com} ; 10, 27 -- Tue, 24 Jan 2012 19:01:59 -0600 (CST) 10, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 10, 27 -- by znl.com (Postfix) with ESMTPA id 9F67347D23C 10, 27 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 19:01:59 -0600 (CST) 10, 27 -- Message-ID: {4F1F5487.3000800-at-microscopy.com} 10, 27 -- Date: Tue, 24 Jan 2012 19:01:59 -0600 10, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 27 -- Reply-To: Zhenquan Liu {Zhenquan.Liu-at-asu.edu} 10, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 27 -- MIME-Version: 1.0 10, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 27 -- Subject: [Filtered] Re: [Microscopy] EMs in the Movies 10, 27 -- References: {201201241353.q0ODrs0X026656-at-ns.microscopy.com} 10, 27 -- In-Reply-To: {201201241353.q0ODrs0X026656-at-ns.microscopy.com} 10, 27 -- Content-Type: text/plain; charset=UTF-8; format=flowed 10, 27 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Several of you mentioned Blade Runner, directed by Ridley Scott.
Back in 1994, we were contacted by an assistant to Mr. Scott for lease of our recently purchased, Hitachi H7100 TEM. They were completing construction of a laboratory set for the film, "Crisis in The Hot Zone," and Mr. Scott wanted a functioning TEM, as was described in the book by Richard Preston. The film was to star Robert Redford and Jodie Foster (who was to operate the TEM).
The production assistant contacted Hitachi to pursue the lease of a TEM for the interior shots, but Hitachi informed them the TEM they wanted was to be delivered to SIU that month. After speaking to the assistant, we agreed to have the TEM delivered to the set in Los Angeles, where HItachi technicians would install and make the scope operational for filming. Afterwards, it would be repacked and shipped to SIU for regular installation. We had already dubbed it, The Jodie Foster Microscope………. sigh.
Several weeks went by and we received a FAX from the assistant indicating that the film was being shelved due to script disagreements and since a similar film, Outbreak, was to be released at about the same time. I saw the awful film and was quite disappointed with the science portrayed in the film. Outbreak was fictional; whereas, Hot Zone is based on an actual incident. Steven King is said to have commented that Hot Zone as the scariest thing he ever read.
Supposedly, a more recent film, "Contagion." with Gwyneth Paltrow and Matt Damon "feels very much like the movie that should have been adapted from the Hot Zone. Has anyone seen "Contagion"? I don't think it did well in the box office. Any TEM's in that film?
Cheers,
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE (Happily Retired :-) Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
} Don't know how this topic came up, but of course Blade Runner has a } scene featuring a behind-the counter SEM with no need for sample prep, } used to quickly distinguish a scale of a replicant-snake from a } replicant-fish. Another scene features an image-enhancing device that } might in retrospect be seen as a form of light-field imaging. } } Peter } } ==============================Original
==============================Original Headers============================== 11, 21 -- From bozzola-at-siu.edu Tue Jan 24 21:33:51 2012 11, 21 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0P3XoX0025971 11, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 21:33:50 -0600 11, 21 -- Received: by vcbfl11 with SMTP id fl11so3831256vcb.0 11, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 19:33:50 -0800 (PST) 11, 21 -- MIME-Version: 1.0 11, 21 -- Received: by 10.52.176.104 with SMTP id ch8mr6827335vdc.99.1327462430341; Tue, 11, 21 -- 24 Jan 2012 19:33:50 -0800 (PST) 11, 21 -- Received: by 10.52.188.200 with HTTP; Tue, 24 Jan 2012 19:33:50 -0800 (PST) 11, 21 -- In-Reply-To: {201201250039.q0P0df3J005817-at-ns.microscopy.com} 11, 21 -- References: {201201250039.q0P0df3J005817-at-ns.microscopy.com} 11, 21 -- Date: Tue, 24 Jan 2012 21:33:50 -0600 11, 21 -- Message-ID: {CAKeCJ9yPdyG=9wk5n-q=0626dZHGHnLhTWqOwOBqa_hFA67dCQ-at-mail.gmail.com} 11, 21 -- Subject: Re: [Microscopy] Re: EMs in the Movies 11, 21 -- From: John Bozzola {bozzola-at-siu.edu} 11, 21 -- To: MSAListserver {Microscopy-at-microscopy.com} 11, 21 -- X-Gm-Message-State: ALoCoQlRkH+W9SR6EXqezt0nhSph3zR+tfO3CGhPerWh+B2xok3GRGcoAkYDVh4IAVefCXtzRP5N 11, 21 -- Content-Type: text/plain; charset=UTF-8 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0P3XoX0025971 ==============================End of - Headers==============================
Contagion is on the list that was sent out. It features and FEI Titan. However, I hear that the company only shipped the outer box for filming. It looks like Gwyneth Paltrow never was able to use it!
If we are looking at microscopes on screen, our FEI G2 20 was featured in a TV advert for the University of Phoenix. We had the film crew here for a day. I estimated about 45 people, all the equipment you ever would need for any sort of scene, and a whole day of filming. The shot on the screen was about 2 seconds long.
Working in Los Angels, we sometimes get good deals on used equipment. For example, we have a pink Zeiss light microscope that featured in the first Jurassic Park movie. Apparently pink objects look white on film, so the machine was painted pink.
The "biggest electron microscope on the East Coast" featured in the first "Spiderman" (and where Peter Parker was bitten by the spider) was a model constructed around a statue in the front atrium of the LA Natural History Museum. Is there anyone on the list from the museum? It would be great to get another "back-stage" look at the collection.
The SEM used by Harrison Ford in Blade Runner was supposedly located in the Los Angeles Chinatown. We guessed it took place in the future because it was raining.
Best wishes on your retirement John.
Paul.
X-from Still Sunny California House Research Institute (we changed our name!) 2100 W 3rd St Los Angeles CA 90057.
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Tue 1/24/2012 7:36 PM To: Webster, Paul
Our "Almost Famous" TEM:
Several of you mentioned Blade Runner, directed by Ridley Scott.
Back in 1994, we were contacted by an assistant to Mr. Scott for lease of our recently purchased, Hitachi H7100 TEM. They were completing construction of a laboratory set for the film, "Crisis in The Hot Zone," and Mr. Scott wanted a functioning TEM, as was described in the book by Richard Preston. The film was to star Robert Redford and Jodie Foster (who was to operate the TEM).
The production assistant contacted Hitachi to pursue the lease of a TEM for the interior shots, but Hitachi informed them the TEM they wanted was to be delivered to SIU that month. After speaking to the assistant, we agreed to have the TEM delivered to the set in Los Angeles, where HItachi technicians would install and make the scope operational for filming. Afterwards, it would be repacked and shipped to SIU for regular installation. We had already dubbed it, The Jodie Foster Microscope���. sigh.
Several weeks went by and we received a FAX from the assistant indicating that the film was being shelved due to script disagreements and since a similar film, Outbreak, was to be released at about the same time. I saw the awful film and was quite disappointed with the science portrayed in the film. Outbreak was fictional; whereas, Hot Zone is based on an actual incident. Steven King is said to have commented that Hot Zone as the scariest thing he ever read.
Supposedly, a more recent film, "Contagion." with Gwyneth Paltrow and Matt Damon "feels very much like the movie that should have been adapted from the Hot Zone. Has anyone seen "Contagion"? I don't think it did well in the box office. Any TEM's in that film?
Cheers,
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE (Happily Retired :-) Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
} Don't know how this topic came up, but of course Blade Runner has a } scene featuring a behind-the counter SEM with no need for sample prep, } used to quickly distinguish a scale of a replicant-snake from a } replicant-fish. Another scene features an image-enhancing device that } might in retrospect be seen as a form of light-field imaging. } } Peter } } ==============================Original
==============================Original Headers============================== 11, 21 -- From bozzola-at-siu.edu Tue Jan 24 21:33:51 2012 11, 21 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0P3XoX0025971 11, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 21:33:50 -0600 11, 21 -- Received: by vcbfl11 with SMTP id fl11so3831256vcb.0 11, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 19:33:50 -0800 (PST) 11, 21 -- MIME-Version: 1.0 11, 21 -- Received: by 10.52.176.104 with SMTP id ch8mr6827335vdc.99.1327462430341; Tue, 11, 21 -- 24 Jan 2012 19:33:50 -0800 (PST) 11, 21 -- Received: by 10.52.188.200 with HTTP; Tue, 24 Jan 2012 19:33:50 -0800 (PST) 11, 21 -- In-Reply-To: {201201250039.q0P0df3J005817-at-ns.microscopy.com} 11, 21 -- References: {201201250039.q0P0df3J005817-at-ns.microscopy.com} 11, 21 -- Date: Tue, 24 Jan 2012 21:33:50 -0600 11, 21 -- Message-ID: {CAKeCJ9yPdyG=9wk5n-q=0626dZHGHnLhTWqOwOBqa_hFA67dCQ-at-mail.gmail.com} 11, 21 -- Subject: Re: [Microscopy] Re: EMs in the Movies 11, 21 -- From: John Bozzola {bozzola-at-siu.edu} 11, 21 -- To: MSAListserver {Microscopy-at-microscopy.com} 11, 21 -- X-Gm-Message-State: ALoCoQlRkH+W9SR6EXqezt0nhSph3zR+tfO3CGhPerWh+B2xok3GRGcoAkYDVh4IAVefCXtzRP5N 11, 21 -- Content-Type: text/plain; charset=UTF-8 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0P3XoX0025971 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 21 -- From PWebster-at-hei.org Wed Jan 25 00:12:42 2012 27, 21 -- Received: from heimail.hei.org (heimail.hei.org [12.175.189.159]) 27, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0P6CgJZ012672 27, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 00:12:42 -0600 27, 21 -- Content-class: urn:content-classes:message 27, 21 -- MIME-Version: 1.0 27, 21 -- Content-Type: text/plain; 27, 21 -- charset="iso-8859-1" 27, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 27, 21 -- Subject: FW: [Microscopy] EMs in the Movies 27, 21 -- Date: Tue, 24 Jan 2012 22:12:41 -0800 27, 21 -- Message-ID: {4B5D9B28C1F49548B4D3C429EE0D32E03902EC-at-hi0sml1.hei.org} 27, 21 -- X-MS-Has-Attach: 27, 21 -- X-MS-TNEF-Correlator: 27, 21 -- Thread-Topic: [Microscopy] EMs in the Movies 27, 21 -- Thread-Index: AczbEobzrSDtQiWCSFa4+yzDhXZj0gAFDShx 27, 21 -- References: {201201250336.q0P3aT3e030781-at-ns.microscopy.com} 27, 21 -- From: "Webster, Paul" {PWebster-at-hei.org} 27, 21 -- To: {Microscopy-at-microscopy.com} 27, 21 -- Content-Transfer-Encoding: 8bit 27, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0P6CgJZ012672 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fmr-at-fmrindia.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: fmr-at-fmrindia.org Name: Dr. Vanaja P. Shetty
Organization: The Foundation for Medical Research
Title-Subject: [Filtered] Disposal of working TEM (Microscope)
Message: We want to dispose of a working Transmission Electron Microscope, Model No. JEM – 100S, CODE No. EMI145041, Serial No. 306
Please send in your inquiry to: Attn. Dr. Vanaja P. Shetty Deputy Director The Foundation for Medical Research 84 – A, R. G. Thadani Marg, Worli Mumbai – 400 018 Email: fmr-at-fmrindia.org Phone: 91 - 22 - 24934989 91 - 22 - 24938601 91 - 22 - 24932876
My favourite example is from the X-Files. Scully visits a lab and watches the electron microscopist use a TEM. She then gives Scully an SEM image of a pollen grain or spore and tells her it must be the alien virus. Needless to say, the electron microscopist is involved in a fatal automobile accident that evening.
Dave
-----Original Message----- X-from: David Patton Sent: 24 January 2012 16:50 To: 'john.mitchels-at-gmail.com'
So far thanks to over 60 responses (and a quick trawl of the archives), this is great keep them coming!
We are up to the following:
1. Flash Gordon 2. Independence Day 3. The Man in White Suit 4. Predator 2 5. CSI 6. Spiderman 2 7. Blade Runner 8. NCIS 9. Dexter 10.Contagion 11. Andromeda Strain The Andromeda Strain (1971)and then the remake 2008 tv-miniseries. 12. Quincy 13. X-Files 14.Pres Obama at Intel's Titan 15.Spiderman 1 16.American Pickers 17.The Last Mimzy 18 ALTO MEDIA 'travel' into the matter. 19. special effects for one of the Star Trek 20. Batman 21. Jurassic Park 22. The Bone Collector" analyzes something 23. Avatar 24. The Prisoner (British TV series) 25. The Naked Gun
Thanks for all the responses so far! Once collected I will put together a website and send the link to the list. John
==============================Original Headers============================== 5, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 16:58:38 2012 5, 29 -- Received: from mail-ww0-f49.google.com (mail-ww0-f49.google.com [74.125.82.49]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0NMwbVi012478 5, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 16:58:38 -0600 5, 29 -- Received: by wgbds1 with SMTP id ds1so1409132wgb.18 5, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 14:58:37 -0800 (PST) 5, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 29 -- d=gmail.com; s=gamma; 5, 29 -- h=message-id:date:from:user-agent:mime-version:to:subject 5, 29 -- :content-type:content-transfer-encoding; 5, 29 -- bh=aP2U2a3GEXIy3Ac+0sIUQE7PlyU64nc7MJmzVqtaIKI=; 5, 29 -- b=HBBmIAxh9wDubCjU7tXHA3VAcCgvt3zA6GSrp+oXwasUpEBcMC2nb+FHcDzGhwo173 5, 29 -- QmRjUy/jI+ut4ZSU/SNJIHtsxVxzr4W+SRnm9GN8KTLdrYZ6fUcfhVQz2VwBuwDiyX9V 5, 29 -- ka4eL/Eker+5DBKiRgbLPoEqWbS8bSUGB92TY= 5, 29 -- Received: by 10.180.82.41 with SMTP id f9mr16708034wiy.7.1327359517654; 5, 29 -- Mon, 23 Jan 2012 14:58:37 -0800 (PST) 5, 29 -- Received: from [127.0.0.1] ([87.114.247.49]) 5, 29 -- by mx.google.com with ESMTPS id n5sm27077735wiw.7.2012.01.23.14.58.36 5, 29 -- (version=TLSv1/SSLv3 cipher=OTHER); 5, 29 -- Mon, 23 Jan 2012 14:58:36 -0800 (PST) 5, 29 -- Message-ID: {4F1DE61C.5030804-at-gmail.com} 5, 29 -- Date: Mon, 23 Jan 2012 22:58:36 +0000 5, 29 -- From: John Mitchels {john.mitchels-at-gmail.com} 5, 29 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:9.0) Gecko/20111222 Thunderbird/9.0.1 5, 29 -- MIME-Version: 1.0 5, 29 -- To: Microscopy-at-microscopy.com 5, 29 -- Subject: Updated Movie List 5, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 18, 47 -- From David.Patton-at-uwe.ac.uk Wed Jan 25 09:04:49 2012 18, 47 -- Received: from TX2EHSOBE003.bigfish.com (tx2ehsobe002.messaging.microsoft.com [65.55.88.12]) 18, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0PF4n3B009534 18, 47 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 09:04:49 -0600 18, 47 -- Received: from mail157-tx2-R.bigfish.com (10.9.14.245) by 18, 47 -- TX2EHSOBE003.bigfish.com (10.9.40.23) with Microsoft SMTP Server id 18, 47 -- 14.1.225.23; Wed, 25 Jan 2012 15:04:48 +0000 18, 47 -- Received: from mail157-tx2 (localhost [127.0.0.1]) by 18, 47 -- mail157-tx2-R.bigfish.com (Postfix) with ESMTP id B4B5E160175; Wed, 25 Jan 18, 47 -- 2012 15:04:48 +0000 (UTC) 18, 47 -- X-SpamScore: -18 18, 47 -- X-BigFish: VPS-18(zz1b0aL7f0R542Mzz1202hz31iz8275bhz2fhc1bhc31hc1ah2a8h668h839h944h) 18, 47 -- X-Forefront-Antispam-Report: CIP:164.11.127.42;KIP:(null);UIP:(null);IPV:NLI;H:egen-hub02.campus.ads.uwe.ac.uk;RD:none;EFVD:NLI 18, 47 -- Received-SPF: pass (mail157-tx2: domain of uwe.ac.uk designates 164.11.127.42 as permitted sender) client-ip=164.11.127.42; envelope-from=David.Patton-at-uwe.ac.uk; helo=egen-hub02.campus.ads.uwe.ac.uk ;ds.uwe.ac.uk ; 18, 47 -- Received: from mail157-tx2 (localhost.localdomain [127.0.0.1]) by mail157-tx2 18, 47 -- (MessageSwitch) id 1327503886531203_2690; Wed, 25 Jan 2012 15:04:46 +0000 18, 47 -- (UTC) 18, 47 -- Received: from TX2EHSMHS012.bigfish.com (unknown [10.9.14.235]) by 18, 47 -- mail157-tx2.bigfish.com (Postfix) with ESMTP id 79E5A200046; Wed, 25 Jan 2012 18, 47 -- 15:04:46 +0000 (UTC) 18, 47 -- Received: from egen-hub02.campus.ads.uwe.ac.uk (164.11.127.42) by 18, 47 -- TX2EHSMHS012.bigfish.com (10.9.99.112) with Microsoft SMTP Server (TLS) id 18, 47 -- 14.1.225.23; Wed, 25 Jan 2012 15:04:45 +0000 18, 47 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([169.254.1.129]) by 18, 47 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.127.42]) with mapi; Wed, 25 Jan 2012 18, 47 -- 15:04:42 +0000 18, 47 -- From: David Patton {David.Patton-at-uwe.ac.uk} 18, 47 -- To: "'john.mitchels-at-gmail.com'" {john.mitchels-at-gmail.com} 18, 47 -- CC: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 18, 47 -- Date: Wed, 25 Jan 2012 15:04:42 +0000 18, 47 -- Subject: RE: [Microscopy] Updated Movie List: X-Files Account 18, 47 -- Thread-Topic: [Microscopy] Updated Movie List: X-Files Account 18, 47 -- Thread-Index: AczaItRVHq4WyzhMRtCuoYu/h/3AcgAlTT5AAC50llA= 18, 47 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF43B940BDFB0-at-EGEN-MBX02.campus.ads.uwe.ac.uk} 18, 47 -- References: {201201232300.q0NN0Zko017352-at-ns.microscopy.com} 18, 47 -- {A169BAD2C2DC6D418270CDC03DF5CDF43B940BDF9A-at-EGEN-MBX02.campus.ads.uwe.ac.uk} 18, 47 -- In-Reply-To: {A169BAD2C2DC6D418270CDC03DF5CDF43B940BDF9A-at-EGEN-MBX02.campus.ads.uwe.ac.uk} 18, 47 -- Accept-Language: en-US, en-GB 18, 47 -- Content-Language: en-US 18, 47 -- X-MS-Has-Attach: 18, 47 -- X-MS-TNEF-Correlator: 18, 47 -- acceptlanguage: en-US, en-GB 18, 47 -- Content-Type: text/plain; charset="us-ascii" 18, 47 -- MIME-Version: 1.0 18, 47 -- X-OriginatorOrg: uwe.ac.uk 18, 47 -- Content-Transfer-Encoding: 8bit 18, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0PF4n3B009534 ==============================End of - Headers==============================
out of my files, just as a supplement to your posts:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
There is also an orange column TEM in the TV series {Dexter} . When he's in his little lab. I too have been bored by TV and movies.
More TEM's in movies I say.... actually how about an old VG HB501. I know the one here has more character and versatility than some leading actors and actresses out there and it works for free. The microscopist on the other hand will need food, drink and board. { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {
best regards and wishes,
Wolfgang MUSS SALZBURG-AUSTRIA
----
} -----Ursprüngliche Nachricht----- } Von: john.mitchels-at-gmail.com [mailto:john.mitchels-at-gmail.com] } Gesendet: Montag, 23. Jänner 2012 23:01 } An: Muß Wolfgang } Betreff: [Microscopy] EMs in the Movies } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } Hi Listers } } I have for a long time been keen to find references to EMs in movies } and put them on a website for all to see. Every time I think about it I } promptly forget and the cycle repeats not this time! } } I wondered if anyone has spotted EMs in movies/tv shows/documentaries? } } If so what and where? If you can point out the make, model and any } inaccuracies all the better! } } So far the ones I can remember are: } } 1. Flash Gordon 1936 (Controls of the ship are a TEM) } 2. Independence Day in Area 51 scene } 3. The Man in White Suit } 4. Predator 2 } 5. CSI had a Hitachi S3400 once I recall } 6. There was a fake one in Spiderman 2 } 7. Blade Runner } } Any more would be greatly appreciated including those not in English! } } Regards } John } } } . } } ==============================Original } Headers============================== } 9, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 15:59:16 2012 } 9, 29 -- Received: from mail-ww0-f49.google.com (mail-ww0- } f49.google.com [74.125.82.49]) } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id q0NLxGtL010271 } 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 15:59:16 } -0600 } 9, 29 -- Received: by wgbds1 with SMTP id ds1so1372983wgb.18 } 9, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2012 } 13:59:15 -0800 (PST) } 9, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 29 -- d=gmail.com; s=gamma; } 9, 29 -- h=message-id:date:from:user-agent:mime- } version:to:subject } 9, 29 -- :content-type:content-transfer-encoding; } 9, 29 -- bh=Ba4Sdz+tb/IFyn9mxcOj+s3UNCp0MilqOz/HQ13Ssj8=; } 9, 29 -- } b=L8Ld1beKC4vs5R3bY4RCUhI0X0RMXmT2MuJuH1/WpJTViudtsP70vIPIVirnCob/sc } 9, 29 -- } Rbnz27D1oogAIyvhuRWv/ADcZNe/CJg8vsMPlRBcqgTZJnne4dy/xSrZAmTNjqXWAp2f } 9, 29 -- UxwFAe6RdM4JSInulGqbxS63niVp0N/bhiVuc= } 9, 29 -- Received: by 10.180.95.1 with SMTP id } dg1mr8530030wib.21.1327355955629; } 9, 29 -- Mon, 23 Jan 2012 13:59:15 -0800 (PST) } 9, 29 -- Received: from [127.0.0.1] ([87.114.247.49]) } 9, 29 -- by mx.google.com with ESMTPS id } n3sm45503712wiz.9.2012.01.23.13.59.14 } 9, 29 -- (version=TLSv1/SSLv3 cipher=OTHER); } 9, 29 -- Mon, 23 Jan 2012 13:59:14 -0800 (PST) } 9, 29 -- Message-ID: {4F1DD832.1070806-at-gmail.com} } 9, 29 -- Date: Mon, 23 Jan 2012 21:59:14 +0000 } 9, 29 -- From: John Mitchels {john.mitchels-at-gmail.com} } 9, 29 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; rv:9.0) } Gecko/20111222 Thunderbird/9.0.1 } 9, 29 -- MIME-Version: 1.0 } 9, 29 -- To: Microscopy-at-microscopy.com } 9, 29 -- Subject: EMs in the Movies } 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 9, 29 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 12, 39 -- From W.Muss-at-salk.at Wed Jan 25 09:46:45 2012 12, 39 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 12, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0PFkjRK027114 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 09:46:45 -0600 12, 39 -- Received: from localhost (localhost [127.0.0.1]) 12, 39 -- by hermes.salk.at (Postfix) with ESMTP id 09649C3897 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 16:46:44 +0100 (CET) 12, 39 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 12, 39 -- Received: from hermes.salk.at ([127.0.0.1]) 12, 39 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 12, 39 -- with ESMTP id wuyRDIuDymef for {microscopy-at-microscopy.com} ; 12, 39 -- Wed, 25 Jan 2012 16:46:43 +0100 (CET) 12, 39 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 12, 39 -- by hermes.salk.at (Postfix) with ESMTP id 96081C384B 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 16:46:43 +0100 (CET) 12, 39 -- Received: from n2rz123.lksdom21.lks.local ([192.168.101.123]) 12, 39 -- by N2EX199.lks.local with ESMTP; 25 Jan 2012 16:47:51 +0100 12, 39 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.133]) by n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.4675); 12, 39 -- Wed, 25 Jan 2012 16:46:39 +0100 12, 39 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 39 -- Content-class: urn:content-classes:message 12, 39 -- MIME-Version: 1.0 12, 39 -- Content-Type: text/plain; 12, 39 -- charset="iso-8859-1" 12, 39 -- Subject: [Microscopy] Re: EMs in the Movies 12, 39 -- Date: Wed, 25 Jan 2012 16:46:14 +0100 12, 39 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062F3359F-at-N1RZ116.lksdom21.lks.local} 12, 39 -- In-Reply-To: {201201232201.q0NM1DDU015005-at-ns.microscopy.com} 12, 39 -- X-MS-Has-Attach: 12, 39 -- X-MS-TNEF-Correlator: 12, 39 -- Thread-Topic: [Microscopy] Re: EMs in the Movies 12, 39 -- Thread-Index: AczaGokvTQONJQfiT96rTSceROzv4wBXcOlQ 12, 39 -- References: {201201232201.q0NM1DDU015005-at-ns.microscopy.com} 12, 39 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 12, 39 -- To: {microscopy-at-microscopy.com} 12, 39 -- X-OriginalArrivalTime: 25 Jan 2012 15:46:39.0491 (UTC) FILETIME=[87080530:01CCDB78] 12, 39 -- X-Scanned-By: SALK-Content-Filter 12, 39 -- Content-Transfer-Encoding: 8bit 12, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0PFkjRK027114 ==============================End of - Headers==============================
What I take from this story is that University of Phoenix wants to portray that they have electron microscopes, yet they have no such thing at any of their many campuses.
Contagion is on the list that was sent out. It features and FEI Titan. However, I hear that the company only shipped the outer box for filming. It looks like Gwyneth Paltrow never was able to use it!
If we are looking at microscopes on screen, our FEI G2 20 was featured in a TV advert for the University of Phoenix. We had the film crew here for a day. I estimated about 45 people, all the equipment you ever would need for any sort of scene, and a whole day of filming. The shot on the screen was about 2 seconds long.
Working in Los Angels, we sometimes get good deals on used equipment. For example, we have a pink Zeiss light microscope that featured in the first Jurassic Park movie. Apparently pink objects look white on film, so the machine was painted pink.
The "biggest electron microscope on the East Coast" featured in the first "Spiderman" (and where Peter Parker was bitten by the spider) was a model constructed around a statue in the front atrium of the LA Natural History Museum. Is there anyone on the list from the museum? It would be great to get another "back-stage" look at the collection.
The SEM used by Harrison Ford in Blade Runner was supposedly located in the Los Angeles Chinatown. We guessed it took place in the future because it was raining.
Best wishes on your retirement John.
Paul.
X-from Still Sunny California House Research Institute (we changed our name!) 2100 W 3rd St Los Angeles CA 90057.
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Tue 1/24/2012 7:36 PM To: Webster, Paul
Our "Almost Famous" TEM:
Several of you mentioned Blade Runner, directed by Ridley Scott.
Back in 1994, we were contacted by an assistant to Mr. Scott for lease of our recently purchased, Hitachi H7100 TEM. They were completing construction of a laboratory set for the film, "Crisis in The Hot Zone," and Mr. Scott wanted a functioning TEM, as was described in the book by Richard Preston. The film was to star Robert Redford and Jodie Foster (who was to operate the TEM).
The production assistant contacted Hitachi to pursue the lease of a TEM for the interior shots, but Hitachi informed them the TEM they wanted was to be delivered to SIU that month. After speaking to the assistant, we agreed to have the TEM delivered to the set in Los Angeles, where HItachi technicians would install and make the scope operational for filming. Afterwards, it would be repacked and shipped to SIU for regular installation. We had already dubbed it, The Jodie Foster MicroscopeÄ?¦Ä?¦Ä?¦. sigh.
Several weeks went by and we received a FAX from the assistant indicating that the film was being shelved due to script disagreements and since a similar film, Outbreak, was to be released at about the same time. I saw the awful film and was quite disappointed with the science portrayed in the film. Outbreak was fictional; whereas, Hot Zone is based on an actual incident. Steven King is said to have commented that Hot Zone as the scariest thing he ever read.
Supposedly, a more recent film, "Contagion." with Gwyneth Paltrow and Matt Damon "feels very much like the movie that should have been adapted from the Hot Zone. Has anyone seen "Contagion"? I don't think it did well in the box office. Any TEM's in that film?
Cheers,
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE (Happily Retired :-) Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÄ€ 62901 Phone: 618-453-3730
} Don't know how this topic came up, but of course Blade Runner has a } scene featuring a behind-the counter SEM with no need for sample prep, } used to quickly distinguish a scale of a replicant-snake from a } replicant-fish. Another scene features an image-enhancing device that } might in retrospect be seen as a form of light-field imaging. } } Peter } } ==============================Original
==============================Original Headers============================== 11, 21 -- From bozzola-at-siu.edu Tue Jan 24 21:33:51 2012 11, 21 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0P3XoX0025971 11, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 21:33:50 -0600 11, 21 -- Received: by vcbfl11 with SMTP id fl11so3831256vcb.0 11, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2012 19:33:50 -0800 (PST) 11, 21 -- MIME-Version: 1.0 11, 21 -- Received: by 10.52.176.104 with SMTP id ch8mr6827335vdc.99.1327462430341; Tue, 11, 21 -- 24 Jan 2012 19:33:50 -0800 (PST) 11, 21 -- Received: by 10.52.188.200 with HTTP; Tue, 24 Jan 2012 19:33:50 -0800 (PST) 11, 21 -- In-Reply-To: {201201250039.q0P0df3J005817-at-ns.microscopy.com} 11, 21 -- References: {201201250039.q0P0df3J005817-at-ns.microscopy.com} 11, 21 -- Date: Tue, 24 Jan 2012 21:33:50 -0600 11, 21 -- Message-ID: {CAKeCJ9yPdyG=9wk5n-q=0626dZHGHnLhTWqOwOBqa_hFA67dCQ-at-mail.gmail.com} 11, 21 -- Subject: Re: [Microscopy] Re: EMs in the Movies 11, 21 -- From: John Bozzola {bozzola-at-siu.edu} 11, 21 -- To: MSAListserver {Microscopy-at-microscopy.com} 11, 21 -- X-Gm-Message-State: ALoCoQlRkH+W9SR6EXqezt0nhSph3zR+tfO3CGhPerWh+B2xok3GRGcoAkYDVh4IAVefCXtzRP5N 11, 21 -- Content-Type: text/plain; charset=UTF-8 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0P3XoX0025971 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 21 -- From PWebster-at-hei.org Wed Jan 25 00:12:42 2012 27, 21 -- Received: from heimail.hei.org (heimail.hei.org [12.175.189.159]) 27, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0P6CgJZ012672 27, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 00:12:42 -0600 27, 21 -- Content-class: urn:content-classes:message 27, 21 -- MIME-Version: 1.0 27, 21 -- Content-Type: text/plain; 27, 21 -- charset="iso-8859-1" 27, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 27, 21 -- Subject: FW: [Microscopy] EMs in the Movies 27, 21 -- Date: Tue, 24 Jan 2012 22:12:41 -0800 27, 21 -- Message-ID: {4B5D9B28C1F49548B4D3C429EE0D32E03902EC-at-hi0sml1.hei.org} 27, 21 -- X-MS-Has-Attach: 27, 21 -- X-MS-TNEF-Correlator: 27, 21 -- Thread-Topic: [Microscopy] EMs in the Movies 27, 21 -- Thread-Index: AczbEobzrSDtQiWCSFa4+yzDhXZj0gAFDShx 27, 21 -- References: {201201250336.q0P3aT3e030781-at-ns.microscopy.com} 27, 21 -- From: "Webster, Paul" {PWebster-at-hei.org} 27, 21 -- To: {Microscopy-at-microscopy.com} 27, 21 -- Content-Transfer-Encoding: 8bit 27, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0P6CgJZ012672 ==============================End of - Headers==============================
==============================Original Headers============================== 38, 28 -- From microtomy-at-gmail.com Wed Jan 25 10:25:51 2012 38, 28 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 38, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0PGPoX0012684 38, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 10:25:50 -0600 38, 28 -- Received: by vcbfl11 with SMTP id fl11so4302680vcb.0 38, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 08:25:50 -0800 (PST) 38, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 38, 28 -- d=gmail.com; s=gamma; 38, 28 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 38, 28 -- :content-type:content-transfer-encoding; 38, 28 -- bh=DJnNwbPn5ru/LwytfYk4MxsEYJ4I9TG8TH9ymfSQBv4=; 38, 28 -- b=U0g5IR1RbkMJX8D2//jvtV3/qFdt6TUYA1iYqRs4GEg8JhS0SDpKlxjawfkKolEs5D 38, 28 -- /i8DtwzRB3PgVkZO9dWlOauU8rqHnYIMZUifPapL2Zyfy5f3y3ypJ1AMO8gVTKaup1v6 38, 28 -- AH/ESBAj4G5xfkuX7f0EFKJcvYIKBcBlzsvhE= 38, 28 -- MIME-Version: 1.0 38, 28 -- Received: by 10.52.92.47 with SMTP id cj15mr10407170vdb.11.1327508750209; Wed, 38, 28 -- 25 Jan 2012 08:25:50 -0800 (PST) 38, 28 -- Received: by 10.220.17.194 with HTTP; Wed, 25 Jan 2012 08:25:50 -0800 (PST) 38, 28 -- In-Reply-To: {201201250613.q0P6Dews014334-at-ns.microscopy.com} 38, 28 -- References: {201201250613.q0P6Dews014334-at-ns.microscopy.com} 38, 28 -- Date: Wed, 25 Jan 2012 10:25:50 -0600 38, 28 -- Message-ID: {CAMWr3Qv=VyzjjiVsHMPP0aT-JqtoiHe3ApDgAbFeQQnzrcJt=A-at-mail.gmail.com} 38, 28 -- Subject: Fwd: [Microscopy] FW: EMs in the Movies 38, 28 -- From: Jay Campbell {microtomy-at-gmail.com} 38, 28 -- To: microscopy {Microscopy-at-microscopy.com} 38, 28 -- Content-Type: text/plain; charset=UTF-8 38, 28 -- Content-Transfer-Encoding: 8bit 38, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0PGPoX0012684 ==============================End of - Headers==============================
} So far thanks to over 60 responses (and a quick trawl of the archives), } this is great keep them coming! } } We are up to the following: } } 1. Flash Gordon } 2. Independence Day } 3. The Man in White Suit } 4. Predator 2 } 5. CSI } 6. Spiderman 2 } 7. Blade Runner } 8. NCIS } 9. Dexter } 10.Contagion } 11. Andromeda Strain The Andromeda Strain (1971)and then the remake 2008 } tv-miniseries. } 12. Quincy } 13. X-Files } 14.Pres Obama at Intel's Titan } 15.Spiderman 1 } 16.American Pickers } 17.The Last Mimzy } 18 ALTO MEDIA 'travel' into the matter. } 19. special effects for one of the Star Trek } 20. Batman } 21. Jurassic Park } 22. The Bone Collector" analyzes something } 23. Avatar } 24. The Prisoner (British TV series) } 25. The Naked Gun } } } Thanks for all the responses so far! Once collected I will put together } a website and send the link to the list. } John
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 26 -- From oshel1pe-at-cmich.edu Wed Jan 25 10:44:22 2012 3, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0PGiMkG029812 3, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 10:44:22 -0600 3, 26 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 3, 26 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q0PGiIaA030305 3, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2012 11:44:22 -0500 3, 26 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 3, 26 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Wed, 25 Jan 3, 26 -- 2012 11:44:21 -0500 3, 26 -- MIME-Version: 1.0 3, 26 -- Message-ID: {a06240807cb45dfb2d50a-at-[141.209.160.249]} 3, 26 -- In-Reply-To: {201201232300.q0NN0cr4017446-at-ns.microscopy.com} 3, 26 -- References: {201201232300.q0NN0cr4017446-at-ns.microscopy.com} 3, 26 -- Date: Wed, 25 Jan 2012 11:44:19 -0500 3, 26 -- To: {Microscopy-at-microscopy.com} 3, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 26 -- Subject: Re: [Microscopy] Updated Movie List 3, 26 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 3, 26 -- X-Originating-IP: [141.209.160.249] 3, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) 3, 26 -- X-Spam-Score: -0.10 () [Hold at 6.00] PORN_RP_NAKED,_L_LLEXCH,RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 3, 26 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 3, 26 -- X-CanItPRO-Stream: default 3, 26 -- X-Canit-Stats-ID: 07GpQIlJj - 7142ebf306db - 20120125 3, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Doctor Who and the Hand of Fear - it's used to examine a stone hand (that comes to life). Not sure of the make, but could be Cambridge Instruments... I'll dig out the DVD.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reganhll-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reganhll-at-gmail.com Name: Johnson
Organization: FMLS
Title-Subject: [Filtered] EM
Message: Dear Listserv members, It would be nice to know what are the advantages of Sodium Cacodylet buffer over other buffer. I looked for literature but most of the books and documents speak about the safety aspects. Greetings, Johnson.
The use of cacodylate as a buffer for EM is more a question of conservationism than anything else. We don't use it because it is the best, we use it because it has been used successfully for more than 50 years and we are too lazy to try something else. Some buffers are better for specific applications, but cacodylate works most of the time for a large panel of samples. Contrary to phosphate buffers, cacodylate does not precipitate in presence of calcium. This is important because it has been shown that calcium stabilizes somes structures during fixation. Tris is not an option because it has an amine and that is what aldehydes react with. Hepes is really a possible alternative, it is a strong buffer and it is compatible with calcium. Personally I go with Hepes and I still have to see a bad fixation due to the buffer (which doesn't mean it is best for all samples of course).
Cheers, Stephane
X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: nizets2-at-yahoo.com Sent: Thursday, January 26, 2012 4:23 AM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reganhll-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reganhll-at-gmail.com Name: Johnson
Organization: FMLS
Title-Subject: [Filtered] EM
Message: Dear Listserv members, It would be nice to know what are the advantages of Sodium Cacodylet buffer over other buffer. I looked for literature but most of the books and documents speak about the safety aspects. Greetings, Johnson.
Hello! I am responsible for a FEI Tecnai G20 which was installed in 2005. As we discussed several times in the list, it is advised to store computer parts for future replacement needs because the computers evolve a lot during the lifetime of a TEM (or was it meant to buy a complete second PC?). I suppose that the interface cards come from FEI themselves, so they will probably be able to provide them for some time. I am not really a genius in computer matters and I would need you opinion about which hardware part of the computer we need to store? Many thanks in advance.
Stephane
==============================Original Headers============================== 2, 34 -- From nizets2-at-yahoo.com Thu Jan 26 04:48:36 2012 2, 34 -- Received: from nm13.bullet.mail.sp2.yahoo.com (nm13.bullet.mail.sp2.yahoo.com [98.139.91.83]) 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q0QAma2A015293 2, 34 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 04:48:36 -0600 2, 34 -- Received: from [98.139.91.66] by nm13.bullet.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 2, 34 -- Received: from [98.139.91.12] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 2, 34 -- Received: from [127.0.0.1] by omp1012.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 2, 34 -- X-Yahoo-Newman-Property: ymail-3 2, 34 -- X-Yahoo-Newman-Id: 763803.14118.bm-at-omp1012.mail.sp2.yahoo.com 2, 34 -- Received: (qmail 74638 invoked by uid 60001); 26 Jan 2012 10:48:35 -0000 2, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1327574915; bh=E0OtdLLP4Dm2TDIa9XkHtZf1P9ODVcUwkECpVoar+t8=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; b=6TmmQstVHFu0bxd6N3D5oZq3ti4hlfMXlJLD5soHX0RNJhtfcWtkMCrkupy1t/VqDYQuAnnrLBGCBbMXZjIgbODi6NZEHGtjXzjayDbnl1KJRhPlnHzs17NIykkdudMqGFnCtlFpe4NsfN0Fn4B91xjbUs9Rl4g3VQUK62Sr5Q8= 2, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 2, 34 -- s=s1024; d=yahoo.com; 2, 34 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; 2, 34 -- b=Ps34eohfubsyB52xQSI5CzduoDc3aKC9+aMQY9DItAkT0Cfst2E0u7jZ0bP2dY7vpssL30uMi4PFcGhXKcfG+M9Qp1ynjXMoWyk9JOWjWdSKgIJvdfz47avV6ff3luGRWO6l7P0k47UPvybnxLT5BvEAlNLE2vJMYjSxsDwPLOY=; 2, 34 -- X-YMail-OSG: rTGjBUoVM1lyS6nm.pneT6c3CTy0cMs58YUPCVfTWVounPn 2, 34 -- GeMSbH9yaTCCKw7KzshMjuXBBI6UvNglDhUfXEz7r7YpKw9aIGIPmlGiwRiM 2, 34 -- _l_419x.VpGjMg0ftOpiIh8VWFBrrJ4jaaMOShTHS4Ix0IPw5KiLxAC1XBBh 2, 34 -- aPlJfqcJNW_TVkXmrUB65WNYGF3WbeY3yMDMyFWLCsG0CHjb4Hg5yBOEYz6X 2, 34 -- mpt4TJrnX2TiI7MKgzKa0q10YEGNb3DfbsBJn8temUQGPQvabbJR5VJDHlNq 2, 34 -- xzkLm1kfi3.A3lvB68LD4U8HlmhHEdXaDZT6N65jjV3DkZNziCnnTnoUgF.v 2, 34 -- JK5mcS3C7LRVq.Iq1IxfANAT45jklKFpsHEgHER1DRYEChnrtVvXOENiebHh 2, 34 -- rd5isc1oEN_hwJt6_c7Dcw5v9oSNWra__4qwBl5CexRrQkEAMVvHjPQbIlJ2 2, 34 -- HK3Y- 2, 34 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Thu, 26 Jan 2012 02:48:35 PST 2, 34 -- X-Mailer: YahooMailWebService/0.8.116.331537 2, 34 -- Message-ID: {1327574915.67749.YahooMailNeo-at-web110806.mail.gq1.yahoo.com} 2, 34 -- Date: Thu, 26 Jan 2012 02:48:35 -0800 (PST) 2, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 2, 34 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 2, 34 -- Subject: which computer parts to store? 2, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 34 -- MIME-Version: 1.0 2, 34 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
Computers are fairly inexpensive (compared to the equipment they run). I would have at least one complete computer standing by in the event of failure.
In my experience, hard drives are the first to go, so back up your hard drives from time to time and have a spare main HD ready to replace the one in the computer.
John Bozzola
On Thu, Jan 26, 2012 at 4:49 AM, {nizets2-at-yahoo.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello! } I am responsible for a FEI Tecnai G20 which was installed in 2005. } As we discussed several times in the list, it is advised to store computer parts for future replacement needs because the computers evolve a lot during the lifetime of a TEM (or was it meant to buy a complete second PC?). } I suppose that the interface cards come from FEI themselves, so they will probably be able to provide them for some time. } I am not really a genius in computer matters and I would need you opinion about which hardware part of the computer we need to store? } Many thanks in advance. } } Stephane } } ==============================Original Headers============================== } 2, 34 -- From nizets2-at-yahoo.com Thu Jan 26 04:48:36 2012 } 2, 34 -- Received: from nm13.bullet.mail.sp2.yahoo.com (nm13.bullet.mail.sp2.yahoo.com [98.139.91.83]) } 2, 34 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q0QAma2A015293 } 2, 34 --     for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 04:48:36 -0600 } 2, 34 -- Received: from [98.139.91.66] by nm13.bullet.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 } 2, 34 -- Received: from [98.139.91.12] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 } 2, 34 -- Received: from [127.0.0.1] by omp1012.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 } 2, 34 -- X-Yahoo-Newman-Property: ymail-3 } 2, 34 -- X-Yahoo-Newman-Id: 763803.14118.bm-at-omp1012.mail.sp2.yahoo.com } 2, 34 -- Received: (qmail 74638 invoked by uid 60001); 26 Jan 2012 10:48:35 -0000 } 2, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1327574915; bh=E0OtdLLP4Dm2TDIa9XkHtZf1P9ODVcUwkECpVoar+t8=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; b=6TmmQstVHFu0bxd6N3D5oZq3ti4hlfMXlJLD5soHX0RNJhtfcWtkMCrkupy1t/VqDYQuAnnrLBGCBbMXZjIgbODi6NZEHGtjXzjayDbnl1KJRhPlnHzs17NIykkdudMqGFnCtlFpe4NsfN0Fn4B91xjbUs9Rl4g3VQUK62Sr5Q8= } 2, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 2, 34 --  s=s1024; d=yahoo.com; } 2, 34 --  h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; } 2, 34 --  b=Ps34eohfubsyB52xQSI5CzduoDc3aKC9+aMQY9DItAkT0Cfst2E0u7jZ0bP2dY7vpssL30uMi4PFcGhXKcfG+M9Qp1ynjXMoWyk9JOWjWdSKgIJvdfz47avV6ff3luGRWO6l7P0k47UPvybnxLT5BvEAlNLE2vJMYjSxsDwPLOY=; } 2, 34 -- X-YMail-OSG: rTGjBUoVM1lyS6nm.pneT6c3CTy0cMs58YUPCVfTWVounPn } 2, 34 --  GeMSbH9yaTCCKw7KzshMjuXBBI6UvNglDhUfXEz7r7YpKw9aIGIPmlGiwRiM } 2, 34 --  _l_419x.VpGjMg0ftOpiIh8VWFBrrJ4jaaMOShTHS4Ix0IPw5KiLxAC1XBBh } 2, 34 --  aPlJfqcJNW_TVkXmrUB65WNYGF3WbeY3yMDMyFWLCsG0CHjb4Hg5yBOEYz6X } 2, 34 --  mpt4TJrnX2TiI7MKgzKa0q10YEGNb3DfbsBJn8temUQGPQvabbJR5VJDHlNq } 2, 34 --  xzkLm1kfi3.A3lvB68LD4U8HlmhHEdXaDZT6N65jjV3DkZNziCnnTnoUgF.v } 2, 34 --  JK5mcS3C7LRVq.Iq1IxfANAT45jklKFpsHEgHER1DRYEChnrtVvXOENiebHh } 2, 34 --  rd5isc1oEN_hwJt6_c7Dcw5v9oSNWra__4qwBl5CexRrQkEAMVvHjPQbIlJ2 } 2, 34 --  HK3Y- } 2, 34 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Thu, 26 Jan 2012 02:48:35 PST } 2, 34 -- X-Mailer: YahooMailWebService/0.8.116.331537 } 2, 34 -- Message-ID: {1327574915.67749.YahooMailNeo-at-web110806.mail.gq1.yahoo.com} } 2, 34 -- Date: Thu, 26 Jan 2012 02:48:35 -0800 (PST) } 2, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 2, 34 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } 2, 34 -- Subject: which computer parts to store? } 2, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 2, 34 -- MIME-Version: 1.0 } 2, 34 -- Content-Type: text/plain; charset=us-ascii } ==============================End of - Headers==============================
-- John J. Bozzola, Ph.D., Retired Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University Carbondale, ILÂ 62901
==============================Original Headers============================== 8, 20 -- From bozzola-at-siu.edu Thu Jan 26 05:39:04 2012 8, 20 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0QBd3uA001328 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 05:39:03 -0600 8, 20 -- Received: by vcbfl11 with SMTP id fl11so350111vcb.0 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 03:39:03 -0800 (PST) 8, 20 -- MIME-Version: 1.0 8, 20 -- Received: by 10.52.173.79 with SMTP id bi15mr752361vdc.31.1327577943459; Thu, 8, 20 -- 26 Jan 2012 03:39:03 -0800 (PST) 8, 20 -- Received: by 10.52.188.200 with HTTP; Thu, 26 Jan 2012 03:39:03 -0800 (PST) 8, 20 -- In-Reply-To: {201201261049.q0QAnMCS016432-at-ns.microscopy.com} 8, 20 -- References: {201201261049.q0QAnMCS016432-at-ns.microscopy.com} 8, 20 -- Date: Thu, 26 Jan 2012 05:39:03 -0600 8, 20 -- Message-ID: {CAKeCJ9w7GvY+iG9938+3T8sJcCbQUnrjJcvRhhYsS+=eBGKncg-at-mail.gmail.com} 8, 20 -- Subject: Re: [Microscopy] which computer parts to store? 8, 20 -- From: John Bozzola {bozzola-at-siu.edu} 8, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 8, 20 -- Content-Type: text/plain; charset=UTF-8 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0QBd3uA001328 ==============================End of - Headers==============================
We have just upgraded hardware computer links and the Windows operating system on a Hitachi S3000N SEM.
The problems will not be same for an FEI except that any interface units used in either the Hitachi or FEI will have been built to a particular PC standard and these change. In terms of maintaining PC hardware therefore it would be worth looking into exactly what FEI boards will link with on a PC interface as well as any limitations dictated by operating systems. It may for instance only be feasible to link FEI boards with an XP system. But the real killer will be the interface which for internal boards was ISA, became EISA and is generally PCI now where even external systems have changed from RS232, various parallel ports, USB (1,2 & 3) and Firewire.
What I'm saying is that you need to know what is the most recent type of PC, operating system and interface that is compatible with the FEI boards and make sure you have a fully functioning system. It's surprising how quickly standards change in PCs and no amount of emulators and patched systems may be able to rescue you when all the old systems have gone.
Incidentally our S3000N now runs on Windows XP which is a vaste improvement on the old Windows NT4.0 system but it's unlikely that we will ever be able to update/upgrade further. Anyway good luck.
Malcolm
Malcolm Haswell Imaging Suite Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
________________________________________ X-from: bozzola-at-siu.edu [bozzola-at-siu.edu] Sent: 26 January 2012 11:47 To: Malcolm Haswell
Computers are fairly inexpensive (compared to the equipment they run). I would have at least one complete computer standing by in the event of failure.
In my experience, hard drives are the first to go, so back up your hard drives from time to time and have a spare main HD ready to replace the one in the computer.
John Bozzola
On Thu, Jan 26, 2012 at 4:49 AM, {nizets2-at-yahoo.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello! } I am responsible for a FEI Tecnai G20 which was installed in 2005. } As we discussed several times in the list, it is advised to store computer parts for future replacement needs because the computers evolve a lot during the lifetime of a TEM (or was it meant to buy a complete second PC?). } I suppose that the interface cards come from FEI themselves, so they will probably be able to provide them for some time. } I am not really a genius in computer matters and I would need you opinion about which hardware part of the computer we need to store? } Many thanks in advance. } } Stephane } } ==============================Original Headers============================== } 2, 34 -- From nizets2-at-yahoo.com Thu Jan 26 04:48:36 2012 } 2, 34 -- Received: from nm13.bullet.mail.sp2.yahoo.com (nm13.bullet.mail.sp2.yahoo.com [98.139.91.83]) } 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q0QAma2A015293 } 2, 34 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 04:48:36 -0600 } 2, 34 -- Received: from [98.139.91.66] by nm13.bullet.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 } 2, 34 -- Received: from [98.139.91.12] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 } 2, 34 -- Received: from [127.0.0.1] by omp1012.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 } 2, 34 -- X-Yahoo-Newman-Property: ymail-3 } 2, 34 -- X-Yahoo-Newman-Id: 763803.14118.bm-at-omp1012.mail.sp2.yahoo.com } 2, 34 -- Received: (qmail 74638 invoked by uid 60001); 26 Jan 2012 10:48:35 -0000 } 2, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1327574915; bh=E0OtdLLP4Dm2TDIa9XkHtZf1P9ODVcUwkECpVoar+t8=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; b=6TmmQstVHFu0bxd6N3D5oZq3ti4hlfMXlJLD5soHX0RNJhtfcWtkMCrkupy1t/VqDYQuAnnrLBGCBbMXZjIgbODi6NZEHGtjXzjayDbnl1KJRhPlnHzs17NIykkdudMqGFnCtlFpe4NsfN0Fn4B91xjbUs9Rl4g3VQUK62Sr5Q8= } 2, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 2, 34 -- s=s1024; d=yahoo.com; } 2, 34 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; } 2, 34 -- b=Ps34eohfubsyB52xQSI5CzduoDc3aKC9+aMQY9DItAkT0Cfst2E0u7jZ0bP2dY7vpssL30uMi4PFcGhXKcfG+M9Qp1ynjXMoWyk9JOWjWdSKgIJvdfz47avV6ff3luGRWO6l7P0k47UPvybnxLT5BvEAlNLE2vJMYjSxsDwPLOY=; } 2, 34 -- X-YMail-OSG: rTGjBUoVM1lyS6nm.pneT6c3CTy0cMs58YUPCVfTWVounPn } 2, 34 -- GeMSbH9yaTCCKw7KzshMjuXBBI6UvNglDhUfXEz7r7YpKw9aIGIPmlGiwRiM } 2, 34 -- _l_419x.VpGjMg0ftOpiIh8VWFBrrJ4jaaMOShTHS4Ix0IPw5KiLxAC1XBBh } 2, 34 -- aPlJfqcJNW_TVkXmrUB65WNYGF3WbeY3yMDMyFWLCsG0CHjb4Hg5yBOEYz6X } 2, 34 -- mpt4TJrnX2TiI7MKgzKa0q10YEGNb3DfbsBJn8temUQGPQvabbJR5VJDHlNq } 2, 34 -- xzkLm1kfi3.A3lvB68LD4U8HlmhHEdXaDZT6N65jjV3DkZNziCnnTnoUgF.v } 2, 34 -- JK5mcS3C7LRVq.Iq1IxfANAT45jklKFpsHEgHER1DRYEChnrtVvXOENiebHh } 2, 34 -- rd5isc1oEN_hwJt6_c7Dcw5v9oSNWra__4qwBl5CexRrQkEAMVvHjPQbIlJ2 } 2, 34 -- HK3Y- } 2, 34 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Thu, 26 Jan 2012 02:48:35 PST } 2, 34 -- X-Mailer: YahooMailWebService/0.8.116.331537 } 2, 34 -- Message-ID: {1327574915.67749.YahooMailNeo-at-web110806.mail.gq1.yahoo.com} } 2, 34 -- Date: Thu, 26 Jan 2012 02:48:35 -0800 (PST) } 2, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 2, 34 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } 2, 34 -- Subject: which computer parts to store? } 2, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 2, 34 -- MIME-Version: 1.0 } 2, 34 -- Content-Type: text/plain; charset=us-ascii } ==============================End of - Headers==============================
-- John J. Bozzola, Ph.D., Retired Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University Carbondale, IL 62901
==============================Original Headers============================== 8, 20 -- From bozzola-at-siu.edu Thu Jan 26 05:39:04 2012 8, 20 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0QBd3uA001328 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 05:39:03 -0600 8, 20 -- Received: by vcbfl11 with SMTP id fl11so350111vcb.0 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 03:39:03 -0800 (PST) 8, 20 -- MIME-Version: 1.0 8, 20 -- Received: by 10.52.173.79 with SMTP id bi15mr752361vdc.31.1327577943459; Thu, 8, 20 -- 26 Jan 2012 03:39:03 -0800 (PST) 8, 20 -- Received: by 10.52.188.200 with HTTP; Thu, 26 Jan 2012 03:39:03 -0800 (PST) 8, 20 -- In-Reply-To: {201201261049.q0QAnMCS016432-at-ns.microscopy.com} 8, 20 -- References: {201201261049.q0QAnMCS016432-at-ns.microscopy.com} 8, 20 -- Date: Thu, 26 Jan 2012 05:39:03 -0600 8, 20 -- Message-ID: {CAKeCJ9w7GvY+iG9938+3T8sJcCbQUnrjJcvRhhYsS+=eBGKncg-at-mail.gmail.com} 8, 20 -- Subject: Re: [Microscopy] which computer parts to store? 8, 20 -- From: John Bozzola {bozzola-at-siu.edu} 8, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 8, 20 -- Content-Type: text/plain; charset=UTF-8 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0QBd3uA001328 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 35 -- From malcolm.haswell-at-sunderland.ac.uk Thu Jan 26 06:38:37 2012 18, 35 -- Received: from max1.sunderland.ac.uk (max1.sunderland.ac.uk [157.228.52.120]) 18, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q0QCcaKO019550 18, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 06:38:36 -0600 18, 35 -- Received: (qmail 14820 invoked from network); 26 Jan 2012 12:38:33 -0000 18, 35 -- Received: from localhost (127.0.0.1) 18, 35 -- by max1.sunderland.ac.uk with SMTP; 26 Jan 2012 12:38:33 -0000 18, 35 -- Received: from max1.sunderland.ac.uk ([127.0.0.1]) 18, 35 -- by localhost (max1.sunderland.ac.uk [127.0.0.1]) (amavisd-new, port 10024) 18, 35 -- with SMTP id 14304-05 for {Microscopy-at-microscopy.com} ; 18, 35 -- Thu, 26 Jan 2012 12:38:29 +0000 (GMT) 18, 35 -- Received: (qmail 14805 invoked by uid 607); 26 Jan 2012 12:38:29 -0000 18, 35 -- Received: from unknown (HELO cas-01.uni.ad.sunderland.ac.uk) (157.228.67.41) 18, 35 -- by max1.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Thu, 26 Jan 2012 12:38:29 +0000 18, 35 -- Received: from MB-01.uni.ad.sunderland.ac.uk ([169.254.1.69]) by 18, 35 -- cas-01.uni.ad.sunderland.ac.uk ([157.228.67.41]) with mapi; Thu, 26 Jan 2012 18, 35 -- 12:38:29 +0000 18, 35 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk} 18, 35 -- To: "Microscopy MSA (Microscopy-at-microscopy.com)" {Microscopy-at-microscopy.com} 18, 35 -- Subject: RE: [Microscopy] Re: which computer parts to store? 18, 35 -- Thread-Topic: [Microscopy] Re: which computer parts to store? 18, 35 -- Thread-Index: AQHM3CReW6SXl7EEL0CyKXEr5HxZlZYekbBE 18, 35 -- Date: Thu, 26 Jan 2012 12:38:29 +0000 18, 35 -- Message-ID: {F258EBB933D8F549902DDFC16829705D32BE385D-at-mb-01.uni.ad.sunderland.ac.uk} 18, 35 -- References: {201201261147.q0QBlN3r017311-at-ns.microscopy.com} 18, 35 -- In-Reply-To: {201201261147.q0QBlN3r017311-at-ns.microscopy.com} 18, 35 -- Accept-Language: en-GB, en-US 18, 35 -- Content-Language: en-GB 18, 35 -- X-MS-Has-Attach: 18, 35 -- X-MS-TNEF-Correlator: 18, 35 -- Content-Type: text/plain; charset="us-ascii" 18, 35 -- MIME-Version: 1.0 18, 35 -- X-Virus-Scanned: by iCritical at max1.sunderland.ac.uk 18, 35 -- Content-Transfer-Encoding: 8bit 18, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0QCcaKO019550 ==============================End of - Headers==============================
In my experience with various instruments (microscopes and otherwise), host computer parts most likely to fail (in rough order of likelihood) are:
1. Hard drives 2. Power supplies 3. Motherboards
As the computer ages, all these parts can be had for next to nothing, if you keep your eye out for them on eBay, second hand shops, and even the dumpster. Having a pile of working hard drives that can replace the working one is a great comfort. Likewise power supplies and motherboards, although I'm lucky enough to have a local repair shop that has been able to repair both in several cases for a very reasonable price. Most often mobos fail either because the CMOS battery dies, or some of the capacitors fail, which is often indicated by a swollen appearance, particularly at the top. These are relatively easy to replace for someone who is handy with a soldering iron. Power supplies are also easily and inexpensively repaired by those who know how.
Having just done this yesterday, the longevity of a computer can be extended by a yearly cleaning of the inside of the computer, esp. fans and heatsinks. I've seen some neglected computers that were *completely* full of dust bunnies and running hotter than a two dollar pistol. Not good for any of the components! I believe regular cleaning is one reason that I'm still running an HP Vectra computer on our EDS system that just had its 12th birthday (knock wood).
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Generally, sodium cacodylate seems to extract less cytoplasm than phosphate buffers. Advantage is more cytoplasm, disadvantage is less contrast because there is more cytoplasm. There may well be (likely are) specific procedures or specimens where cacodylate buffer is better. The arsenic content is likely more important. You might want to have a look at "Biomedical Electron Microscopy" by Maunsbach and Afzelius - they show many EMs comparing different preparatory methods (fixatives, buffers, etc.), embedding resins, and so on. A good book to have around.
Phil
} Message: Dear Listserv members, } It would be nice to know what are the advantages of Sodium Cacodylet } buffer over other buffer. I } looked for literature but most of the books and documents speak } about the safety aspects. } Greetings, } Johnson.
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 27 -- From oshel1pe-at-cmich.edu Thu Jan 26 07:39:14 2012 4, 27 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0QDdDEa021892 4, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 07:39:14 -0600 4, 27 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 4, 27 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q0QDd2I9005572; 4, 27 -- Thu, 26 Jan 2012 08:39:12 -0500 4, 27 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 4, 27 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Thu, 26 Jan 4, 27 -- 2012 08:39:12 -0500 4, 27 -- MIME-Version: 1.0 4, 27 -- Message-ID: {a06240802cb4706d83d67-at-[141.209.160.249]} 4, 27 -- In-Reply-To: {201201260322.q0Q3Mr4I012795-at-ns.microscopy.com} 4, 27 -- References: {201201260322.q0Q3Mr4I012795-at-ns.microscopy.com} 4, 27 -- Date: Thu, 26 Jan 2012 08:39:09 -0500 4, 27 -- To: {Microscopy-at-microscopy.com} 4, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 27 -- Subject: Re: [Microscopy] viaWWW:Sodium Cacodylet buffer 4, 27 -- CC: {reganhll-at-gmail.com} 4, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 27 -- X-Originating-IP: [141.209.160.249] 4, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) 4, 27 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 4, 27 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 27 -- X-CanItPRO-Stream: default 4, 27 -- X-Canit-Stats-ID: 06GqdDcM8 - e93cfd5e6281 - 20120126 4, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
As others already suggested, first priority is to have:
1) Harddrive with "carbon copy" of currently running software - it is very easy to create with DD (Disk Dump) utility; 2) Motherboard; 3) Processor;
But do not assume that interface cards in the PC are made by OEM of the TEM. I am not familiar with Technai specifically, but chances are that most (if not all) cards installed in the control computer are off-the-shelf products. If such adapted card becomes unavailable from its OEM, it will becom impossible (or next to impossible) to get from the FEI. So by all means solicit help from someone who is very familiar with PC hardware and get all the cards sitting on PC bus.
Cheers :) Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 1/26/2012 5:49 AM, nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello! } I am responsible for a FEI Tecnai G20 which was installed in 2005. } As we discussed several times in the list, it is advised to store computer parts for future replacement needs because the computers evolve a lot during the lifetime of a TEM (or was it meant to buy a complete second PC?). } I suppose that the interface cards come from FEI themselves, so they will probably be able to provide them for some time. } I am not really a genius in computer matters and I would need you opinion about which hardware part of the computer we need to store? } Many thanks in advance. } } Stephane } } ==============================Original Headers============================== } 2, 34 -- From nizets2-at-yahoo.com Thu Jan 26 04:48:36 2012 } 2, 34 -- Received: from nm13.bullet.mail.sp2.yahoo.com (nm13.bullet.mail.sp2.yahoo.com [98.139.91.83]) } 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q0QAma2A015293 } 2, 34 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 04:48:36 -0600 } 2, 34 -- Received: from [98.139.91.66] by nm13.bullet.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 } 2, 34 -- Received: from [98.139.91.12] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 } 2, 34 -- Received: from [127.0.0.1] by omp1012.mail.sp2.yahoo.com with NNFMP; 26 Jan 2012 10:48:35 -0000 } 2, 34 -- X-Yahoo-Newman-Property: ymail-3 } 2, 34 -- X-Yahoo-Newman-Id: 763803.14118.bm-at-omp1012.mail.sp2.yahoo.com } 2, 34 -- Received: (qmail 74638 invoked by uid 60001); 26 Jan 2012 10:48:35 -0000 } 2, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1327574915; bh=E0OtdLLP4Dm2TDIa9XkHtZf1P9ODVcUwkECpVoar+t8=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; b=6TmmQstVHFu0bxd6N3D5oZq3ti4hlfMXlJLD5soHX0RNJhtfcWtkMCrkupy1t/VqDYQuAnnrLBGCBbMXZjIgbODi6NZEHGtjXzjayDbnl1KJRhPlnHzs17NIykkdudMqGFnCtlFpe4NsfN0Fn4B91xjbUs9Rl4g3VQUK62Sr5Q8= } 2, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 2, 34 -- s=s1024; d=yahoo.com; } 2, 34 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; } 2, 34 -- b=Ps34eohfubsyB52xQSI5CzduoDc3aKC9+aMQY9DItAkT0Cfst2E0u7jZ0bP2dY7vpssL30uMi4PFcGhXKcfG+M9Qp1ynjXMoWyk9JOWjWdSKgIJvdfz47avV6ff3luGRWO6l7P0k47UPvybnxLT5BvEAlNLE2vJMYjSxsDwPLOY=; } 2, 34 -- X-YMail-OSG: rTGjBUoVM1lyS6nm.pneT6c3CTy0cMs58YUPCVfTWVounPn } 2, 34 -- GeMSbH9yaTCCKw7KzshMjuXBBI6UvNglDhUfXEz7r7YpKw9aIGIPmlGiwRiM } 2, 34 -- _l_419x.VpGjMg0ftOpiIh8VWFBrrJ4jaaMOShTHS4Ix0IPw5KiLxAC1XBBh } 2, 34 -- aPlJfqcJNW_TVkXmrUB65WNYGF3WbeY3yMDMyFWLCsG0CHjb4Hg5yBOEYz6X } 2, 34 -- mpt4TJrnX2TiI7MKgzKa0q10YEGNb3DfbsBJn8temUQGPQvabbJR5VJDHlNq } 2, 34 -- xzkLm1kfi3.A3lvB68LD4U8HlmhHEdXaDZT6N65jjV3DkZNziCnnTnoUgF.v } 2, 34 -- JK5mcS3C7LRVq.Iq1IxfANAT45jklKFpsHEgHER1DRYEChnrtVvXOENiebHh } 2, 34 -- rd5isc1oEN_hwJt6_c7Dcw5v9oSNWra__4qwBl5CexRrQkEAMVvHjPQbIlJ2 } 2, 34 -- HK3Y- } 2, 34 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Thu, 26 Jan 2012 02:48:35 PST } 2, 34 -- X-Mailer: YahooMailWebService/0.8.116.331537 } 2, 34 -- Message-ID: {1327574915.67749.YahooMailNeo-at-web110806.mail.gq1.yahoo.com} } 2, 34 -- Date: Thu, 26 Jan 2012 02:48:35 -0800 (PST) } 2, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 2, 34 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } 2, 34 -- Subject: which computer parts to store? } 2, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 2, 34 -- MIME-Version: 1.0 } 2, 34 -- Content-Type: text/plain; charset=us-ascii } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 41 -- From vray-at-partbeamsystech.com Thu Jan 26 07:39:37 2012 6, 41 -- Received: from nm5-vm0.access.bullet.mail.mud.yahoo.com (nm5-vm0.access.bullet.mail.mud.yahoo.com [66.94.237.155]) 6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q0QDdbsS022502 6, 41 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 07:39:37 -0600 6, 41 -- Received: from [66.94.237.199] by nm5.access.bullet.mail.mud.yahoo.com with NNFMP; 26 Jan 2012 13:39:37 -0000 6, 41 -- Received: from [98.139.244.32] by tm10.access.bullet.mail.mud.yahoo.com with NNFMP; 26 Jan 2012 13:39:37 -0000 6, 41 -- Received: from [127.0.0.1] by smtp108.biz.mail.bf1.yahoo.com with NNFMP; 26 Jan 2012 13:39:36 -0000 6, 41 -- X-Yahoo-Newman-Id: 937657.87026.bm-at-smtp108.biz.mail.bf1.yahoo.com 6, 41 -- X-Yahoo-Newman-Property: ymail-3 6, 41 -- X-YMail-OSG: nuuEBSkVM1kf7p_Kgv.Co6nh6cuPq7ihHs2neIeWoV_y0UA 6, 41 -- 9IyezNm252w7HmdjmFdCc1fjQoac.KWT2ueu_pN_h2hddcbXLvEGer.8_OYT 6, 41 -- sIaShAE1YCfu1I0s4l2qC.ui9fzdFxI1UhmfXvB.naPexMBia093DMkU.uRM 6, 41 -- q._e9DNIYy.sA0VU9oQc.a5Po5vUl0QEuwAUxW0qj1MimFJs6ixwqS2IMCDl 6, 41 -- HGrd40KJ3cWBp6ZJqTYUskZlZueQgsyxLuVz.6hqY6udu2cH6U01QyGSLUbP 6, 41 -- 72eIPodPns0IlXVPJPTQGcyVGUIvyCkLIYbLr33.Q6w_qi0mLaTuiNUJgibR 6, 41 -- 9aiHrs9m6Uz6sDdXxX5v3BdhE.hEIXM2VCg7V06rHNU.CnmtwuOVPBnZf1CM 6, 41 -- jKDi_iJgsNumX5avqlzNmqUPvEWMzc2RrtgmVEQRAhCHcJnkhnB643oX.qC4 6, 41 -- E7a3Hw49DwTDdg0jvrWIxbZQY97suu0DNgl13k7Nmr2v1JnAaiENpmwxoYvW 6, 41 -- EdLS3ImUuX3ml5dmv5y2PHkdYcHcm1Ge.fgWCcLRgxoSB4CFQWhHg0YCtqrJ 6, 41 -- eLJuOLETkHWLwYnhh5I3suQk4mDR4mQiveRRx1cJY5AddecO.Kzih26BpbWQ 6, 41 -- GxiLtrI4WSst3YeHUJgYTwerBD.EzTwJlQN8e6ZDBdhALV7zARVl44UJQxhu 6, 41 -- txxKX9X.JFCETBBVLorqe_yImDfSTrhAAHDIlMFPbsIlu3sMXWQQN3cEHVEg 6, 41 -- QNvYj5Us1ruqmEZtpaZK7aLS2WE1rOf6.Mu7gWDA2uRZWE__QIdWwoGTPdTo 6, 41 -- uDAw8cixFPI1yzIB_F_WXDtosxz_4wdVbXdiMcwdPQaWj5zQVLX0qVL.f7Vd 6, 41 -- LMLrcoSlPZX0- 6, 41 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 41 -- Received: from [192.168.2.4] (vray-at-96.233.126.242 with plain) 6, 41 -- by smtp108.biz.mail.bf1.yahoo.com with SMTP; 26 Jan 2012 05:39:36 -0800 PST 6, 41 -- Message-ID: {4F215799.3090707-at-partbeamsystech.com} 6, 41 -- Date: Thu, 26 Jan 2012 08:39:37 -0500 6, 41 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 6, 41 -- Reply-To: vray-at-partbeamsystech.com 6, 41 -- Organization: PBS&T 6, 41 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:9.0) Gecko/20111222 Thunderbird/9.0.1 6, 41 -- MIME-Version: 1.0 6, 41 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com 6, 41 -- Subject: Re: [Microscopy] which computer parts to store? 6, 41 -- References: {201201261049.q0QAnSwM016637-at-ns.microscopy.com} 6, 41 -- In-Reply-To: {201201261049.q0QAnSwM016637-at-ns.microscopy.com} 6, 41 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Stephane As John Bozzola wisely recommends, have at least one complete computer standing by with a ghost HD. Don't be sure that company will keep any spare cards for a long time. I have no experience with FEI but JEOL was unable to provide a spare graphic card just 7 years after the model (JSM5600LV) appeared. Make sure your spare computer is in a working condition by putting it on from time to time. This frenzy of consumarism wlll have no end and we have to get prepared for the worse.. Best -yorgos
----- Original Message ----- X-from: {nizets2-at-yahoo.com} To: {eikonika-at-otenet.gr} Sent: Thursday, January 26, 2012 12:54 PM
Stephane raised a great question and there have been several great suggestions. We have a lab full of older FEI SEM and TEM microscopes and have discussed this with our field service engineer. Our engineer tells us that FEI learned from the problems they had with the availability of components in early computer-controlled microscopes because so much functionality was on the microscope PC. I think FEI was not unique in encountering this problem. The engineer told us that FEI made a concerted effort to move as much functionality as possible into the microscope and to use multiple fast ethernet connections to communicate with the PC. This makes upgrading the PC easier because fewer custom boards are used. We have an older dual-beam FIB where component availability has been a real issue. We always get scrutiny from our IS folks because we cannot move out of unsupported operating systems...
I would have a frank conversation with FEI's service specialist about where your particular microscope is on this development timeline and what your particular exposure is to part availability. At a minimum, I would always have a spare hard drive with a disk image of a working system configured the way you want sitting in a sealed container in the lab. This has saved our bacon many times...
Best regards, John Minter Eastman Kodak
==============================Original Headers============================== 3, 28 -- From jrminter-at-gmail.com Thu Jan 26 08:38:46 2012 3, 28 -- Received: from mail-ww0-f49.google.com (mail-ww0-f49.google.com [74.125.82.49]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0QEcjMQ008609 3, 28 -- for {Microscopy-at-Microscopy.Com} ; Thu, 26 Jan 2012 08:38:45 -0600 3, 28 -- Received: by wgbds1 with SMTP id ds1so443308wgb.18 3, 28 -- for {Microscopy-at-Microscopy.Com} ; Thu, 26 Jan 2012 06:38:45 -0800 (PST) 3, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 28 -- d=gmail.com; s=gamma; 3, 28 -- h=mime-version:sender:in-reply-to:references:date 3, 28 -- :x-google-sender-auth:message-id:subject:from:to:content-type; 3, 28 -- bh=7i4Gtw1WWPrZrt7Dx+QnGW8z2PEA8edtpHeHyHMf58A=; 3, 28 -- b=rNDEVbVgD5d1M2GUT8em+q12tD/yFqdvH4FF30pY8hcRPaOh6gk8OzQJV43B5kHTPg 3, 28 -- HK5QPOCweNL1NNU6JekZ9YAasIyh2yP/QqRG71nmo9l65NWjAaOJhMhJFwqId0H+TEzl 3, 28 -- xPP9bXEFneOU9/PLHlzBKfMN2NtVLQBoKYnP0= 3, 28 -- MIME-Version: 1.0 3, 28 -- Received: by 10.180.83.72 with SMTP id o8mr4596147wiy.22.1327588725187; Thu, 3, 28 -- 26 Jan 2012 06:38:45 -0800 (PST) 3, 28 -- Sender: jrminter-at-gmail.com 3, 28 -- Received: by 10.216.175.131 with HTTP; Thu, 26 Jan 2012 06:38:44 -0800 (PST) 3, 28 -- In-Reply-To: {201201261238.q0QCch3x019658-at-ns.microscopy.com} 3, 28 -- References: {201201261238.q0QCch3x019658-at-ns.microscopy.com} 3, 28 -- Date: Thu, 26 Jan 2012 09:38:44 -0500 3, 28 -- X-Google-Sender-Auth: uTnuixfkiJbl1KdosSSKmLJfx5Y 3, 28 -- Message-ID: {CABq4i1MevZ7SWhFnQ6bJBcRw_WnhcSdbmsk6ViorQ-m7bhJoZg-at-mail.gmail.com} 3, 28 -- Subject: Re: [Microscopy] which computer parts to store? 3, 28 -- From: John Minter {jrminter-at-rochester.rr.com} 3, 28 -- To: Microscopy-at-Microscopy.Com 3, 28 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Dear All, I am just in my first experience of doing wet cryo ultramicrotome on diamond sectioning of a PE compound containg ca. 1um size particles. First I tried dry sectioning on a glass knife at -120°C but the cuts came down rolled up very small in diameter. I had no idea how to smooth them. Cutting seems impossible below 100 nm. Wet cryo cuts now are coming off in 70% DMSO at -50°C at least somewhat flat but very compressed. Thickness cutting is now 50 nm. Can you give my some basic advice what I can improve (might be a lot ;-) ).
Best wishes, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
This is a 3-day course aimed at providing instruction on how to qualify immunogold label from images collected using transmission electron microscopy.
During the past decade, new ways of quantifying gold labeling within cells have been devised. Their efficiency and validity rely on sound principles of specimen sampling, event counting and inferential statistics. These stereological tools will be introduced in lectures as well as by using practical examples. Participants will be provided with practical exercises to illustrate gold counting methods, which can be taken away for future reference.
This is an intensive, hands-on course that is organized by an expert electron microscopist and immunologist, and taught by the developer of many of these stereological tools, who is also an expert stereologist.
For further details contact: Paul Webster, Ph.D. House Research Institute 2100 West Third Street Los Angeles CA 90057
Or check out http://immunogold.houseresearch.org
==============================Original Headers============================== 3, 18 -- From PWebster-at-hei.org Thu Jan 26 14:09:08 2012 3, 18 -- Received: from heimail.hei.org (heimail.hei.org [12.175.189.159]) 3, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0QK98XT018123 3, 18 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2012 14:09:08 -0600 3, 18 -- Received: from 10.10.42.25 ([10.10.42.25]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 3, 18 -- Thu, 26 Jan 2012 20:09:07 +0000 3, 18 -- User-Agent: Microsoft-Entourage/12.27.0.100910 3, 18 -- Date: Thu, 26 Jan 2012 12:09:06 -0800 3, 18 -- Subject: Quantifying immunogold label on EM thin sections 3, 18 -- From: "Webster, Paul" {pwebster-at-hei.org} 3, 18 -- To: {microscopy-at-microscopy.com} 3, 18 -- Message-ID: {CB46F2E2.188C6%pwebster-at-hei.org} 3, 18 -- Thread-Topic: Quantifying immunogold label on EM thin sections 3, 18 -- Thread-Index: AczcZlsYxf74KJLMQS6n8ekQ8RvqgA== 3, 18 -- Mime-version: 1.0 3, 18 -- Content-type: text/plain; 3, 18 -- charset="US-ASCII" 3, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both numan.hardy1-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: numan.hardy1-at-gmail.com Name: Prof. Numan Hardy
Organization: Curtin University, Australia
Title-Subject: [Filtered] EVO 18 SEM from ZEISS Message: Dear All,
Does any one have an idea about the cheap EVO 18 SEM manufactured by Zeiss. I have limited budget of US $ 80K and interested to buy either a second hand unit or EVO 18. Please let me know if anybody has used this is Australia and from where I can buy it.
Please also comment on the quality of this model as I could not find any details on the web but have seen this system in China.
The Department of Materials at the University of Oxford (UK) seeks a specialist facility technical manager of the highest calibre with the ability to provide strategic leadership for a large, world-class electron microscope facility.
Please follow the link: https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.jobspec?p_id=101946 for more information
Closing date for applications is 24th February.
==============================Original Headers============================== 7, 30 -- From gabriella.chapman-at-materials.ox.ac.uk Fri Jan 27 03:15:17 2012 7, 30 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0R9FH29017562 7, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Jan 2012 03:15:17 -0600 7, 30 -- Received: from smtp3.nexus.ox.ac.uk ([163.1.154.137] helo=exht03.ad.oak.ox.ac.uk) 7, 30 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.75) 7, 30 -- (envelope-from {gabriella.chapman-at-materials.ox.ac.uk} ) 7, 30 -- id 1Rqhts-0005Ch-Ub 7, 30 -- for Microscopy-at-microscopy.com; Fri, 27 Jan 2012 09:15:16 +0000 7, 30 -- Received: from EXMBX06.ad.oak.ox.ac.uk ([169.254.2.23]) by 7, 30 -- exht03.ad.oak.ox.ac.uk ([163.1.154.54]) with mapi; Fri, 27 Jan 2012 09:15:16 7, 30 -- +0000 7, 30 -- From: Gabriella Chapman {gabriella.chapman-at-materials.ox.ac.uk} 7, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 30 -- Date: Fri, 27 Jan 2012 09:15:17 +0000 7, 30 -- Subject: Job opening: E M Facility Technical Manager, University of Oxford, 7, 30 -- UK 7, 30 -- Thread-Topic: Job opening: E M Facility Technical Manager, University of 7, 30 -- Oxford, UK 7, 30 -- Thread-Index: Aczc1C9X2KryS5UxSDGcE4K4jJVhjA== 7, 30 -- Message-ID: {514B8B67DC93AE42BCEA24880C8BC9C525869FA05F-at-EXMBX06.ad.oak.ox.ac.uk} 7, 30 -- Accept-Language: en-US, en-GB 7, 30 -- Content-Language: en-US 7, 30 -- X-MS-Has-Attach: 7, 30 -- X-MS-TNEF-Correlator: 7, 30 -- acceptlanguage: en-US, en-GB 7, 30 -- Content-Type: text/plain; charset="iso-8859-1" 7, 30 -- MIME-Version: 1.0 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0R9FH29017562 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stempies-at-muohio.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Resins for immunocytochemistry
Message: Dear Listserv,
I am looking at immunogold labeling proteins in Arabidopsis chloroplasts for TEM and was wondering if anyone on the Listserv had experience with the differences in immunocytochemistry resins. I'm interested in knowing how well Unicryl, LR White, and LR Gold have performed in other people's experience with immunogold labeling.
I don't know if this was mentioned earlier: My favorite EM in recent movies is in "Invasion" with Nicole Kidman (2007). She plays a doctor trying to figure out a virus, and they use an FEI TEM to look at the virus. The interesting part is that when they move the digital camera in (side-mount), the next scene shows ... color images of red blood cells swimming in a liquid (blood) and being attacked by the virus. I guess you can call that "poetic license". I am certainly not claiming that our cameras (which they use) can do that :-)
Mike Bode, Ph.D. ResAlta Research Technologies Corp.
Does anyone have experience with the Tucsen 9 MP microscope camera? Is the computer interface easy to use? Is it easy to change image resolution back and fourth for focusing and image capture? Is the measuring software adequate? Can you set the white balance manually and have it remain constant? How about overall image and video quality? If you know of any issues with this camera, please let me know on or off list.
==============================Original Headers============================== 1, 22 -- From common-at-msu.edu Sun Jan 29 13:24:37 2012 1, 22 -- Received: from sys55.mail.msu.edu (sys55.mail.msu.edu [35.9.75.235]) 1, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0TJOb3Q021664 1, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 29 Jan 2012 13:24:37 -0600 1, 22 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; d=msu.edu; s=mail; 1, 22 -- h=Content-Transfer-Encoding:Content-Type:Subject:To:MIME-Version:From:Date:Message-ID; bh=CSBLXnXex8HyusLRTF5zdUFy1ZoHGhbLIfaKmGEtFKk=; 1, 22 -- b=BRYfgfbX1WHZKf++Te15oud+I/gv95+ZfPr21EA3ICSqLDYQ5zkSdHVZfRKFwJnKgm/b9z1tHlowI2JLkMxX6qkAiew6J2Ep2RPc2aSCE6a7CbrXvA/7DrGBXhcyDMhDG9L3+iOrKjldkh9uOq3po4KedQajB8uvplOqCed8We4=; 1, 22 -- Received: from 103-255.187-72.tampabay.res.rr.com ([72.187.255.103] helo=[127.0.0.1]) Authenticated ID: common 1, 22 -- by sys55.mail.msu.edu with esmtpsa (Exim 4.75 #3) 1, 22 -- (TLSv1:CAMELLIA256-SHA:256) 1, 22 -- id 1RraMe-00042V-K1 1, 22 -- for Microscopy-at-microscopy.com; Sun, 29 Jan 2012 14:24:36 -0500 1, 22 -- Message-ID: {4F259CEE.7040101-at-msu.edu} 1, 22 -- Date: Sun, 29 Jan 2012 14:24:30 -0500 1, 22 -- From: Ralph Common {common-at-msu.edu} 1, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:9.0) Gecko/20111222 Thunderbird/9.0.1 1, 22 -- MIME-Version: 1.0 1, 22 -- To: Microscopy-at-microscopy.com 1, 22 -- Subject: Tucsen camera 1, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 22 -- Content-Transfer-Encoding: 7bit 1, 22 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************************** } Date: Mon, 30 Jan 2012 10:43:44 -0800 } From: {AssociationManagement-at-microscopy.org} } Reply-To: Jaimie Graham {jaimie_graham-at-cjuhsd.net} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Monday, January 30, } 2012 at 10:43:40 AM. } } realname - Jaimie Graham } Email - jaimie_graham-at-cjuhsd.net } ORGANIZATION - Ontario High School } EDUCATION - 9-12th Grade High School } LOCATION - Ontario High School } SUBJECT_OF_QUESTION - SEM } QUESTION - We have recently received a donated functioning Cambridge } Instrument Stereoscan 360 SEM. I have used an SEM in college, so } being one of the only teachers here on campus with any experience I } have been given the task of figuring out how to put this microscope } together. I was shipped to us in three main pieces and is currently } wrapped in plastic. Most of it put together. Will I be able to } assemble the microscope or do I need to find a company to put it } together? Is it like a computer where it is simple to figure out } what plugs into what or am I in way over my head??? Help! }
==============================Original Headers============================== 1, 24 -- From oshel1pe-at-cmich.edu Mon Jan 30 13:11:28 2012 1, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0UJBSHX031882 1, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jan 2012 13:11:28 -0600 1, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 1, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q0UJBRK7013834 1, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jan 2012 14:11:28 -0500 1, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 1, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Mon, 30 Jan 1, 24 -- 2012 14:11:27 -0500 1, 24 -- MIME-Version: 1.0 1, 24 -- Message-ID: {a06240807cb4c9b9c579a-at-[141.209.160.249]} 1, 24 -- Date: Mon, 30 Jan 2012 14:11:24 -0500 1, 24 -- To: {Microscopy-at-microscopy.com} 1, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 24 -- Subject: Assembling a SEM 1, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 1, 24 -- X-Originating-IP: [141.209.160.249] 1, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) 1, 24 -- X-Spam-Score: -0.60 () [Hold at 6.00] _L_LLEXCH,RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 1, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 24 -- X-CanItPRO-Stream: default 1, 24 -- X-Canit-Stats-ID: 07GrTbsFq - df144c899b89 - 20120130 1, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Re: donated Cambridge Instruments StereoScan 360 SEM: this looks like a good instrument and it should be nice to have in School, but it's not as easy as a computer to fit together. I would recommend getting professionals to put it together and switch it on. You are going to have to get a Safety certificate before you can use it in class, e.g. an SEM like this does produce X-rays.
The three parts are probably: (1) the electron optical column (heavy metal thing with suspension and a vacuum pumping system), (2) the control electronics console, with monitors and lots of cables around the back and (3) a crate with e.g. high voltage cable, mechanical pump(s), UPS battery system, X-ray detector, computer and accessories.
Try to find manuals, circuit diagrams and ideally installation/performance data; next look into a service company - try searching online. In the best case and with luck you might find someone who can fix things and get the SEM working for around $10K (about a weeks work).
Good luck -- Robert Keyse EM Facility Lehigh University
==============================Original Headers============================== 5, 23 -- From rok210-at-lehigh.edu Mon Jan 30 14:33:19 2012 5, 23 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0UKXJmc017691 5, 23 -- for {microscopy-at-microscopy.com} ; Mon, 30 Jan 2012 14:33:19 -0600 5, 23 -- Received: from [127.0.0.1] (Dyn055015.mat.Lehigh.EDU [128.180.55.15]) 5, 23 -- (authenticated bits=0) 5, 23 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id q0UKXHwB011726 5, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 5, 23 -- Mon, 30 Jan 2012 15:33:18 -0500 5, 23 -- Message-ID: {4F26FE8D.4020206-at-lehigh.edu} 5, 23 -- Date: Mon, 30 Jan 2012 15:33:17 -0500 5, 23 -- From: Robert Keyse {rok210-at-lehigh.edu} 5, 23 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:9.0) Gecko/20111222 Thunderbird/9.0.1 5, 23 -- MIME-Version: 1.0 5, 23 -- To: jaimie_graham-at-cjuhsd.net 5, 23 -- CC: microscopy-at-microscopy.com 5, 23 -- Subject: Assembling an SEM 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-Antivirus: avast! (VPS 120130-0, 01/30/2012), Outbound message 5, 23 -- X-Antivirus-Status: Clean 5, 23 -- X-Virus-Scanned: clamav-milter 0.97.3 at rain.cc.lehigh.edu 5, 23 -- X-Virus-Status: Clean ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nesmicroscopy-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: nesmicroscopy-at-gmail.com Name: NESM
Organization: The New England Society for Microscopy
Message: Join NESM for our February Dinner Meeting hosted by Saint-Gobainon February 15, 2012 -at- 5:30PM. The meeting is composed of a Saint-Gobain facility tour, dinner, and two technical talks (see below for talk information). Register now on our website nesmicroscopy.org . You can also keep up with NESM activities by checking us out of Facebook and Twitter.
Technical Talks:
"Crystals as Stacked Layers and the Infinite World of Intergrowths", Charles Bateman, Ph.D., Saint-Gobain, Northboro, MA
"Microscopy tools: visual resolution of insect eyes", Paloma Gonzalez Bellido, Ph.D., Marine Biological Laboratory, Woods Hole, MA
The Hooke College of Applied Sciences, located in Westmont, IL, is offering its scanning electron microscopy short course March 26-30, 2012. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment. For further SEM training details and registration information, please follow the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax) www.hookecollege.com
==============================Original Headers============================== 9, 26 -- From CGorman-at-hookecollege.com Tue Jan 31 11:54:07 2012 9, 26 -- Received: from mail.mccrone.com (mail.mccrone.com [12.54.22.114]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0VHs7fE025979 9, 26 -- for {microscopy-at-microscopy.com} ; Tue, 31 Jan 2012 11:54:07 -0600 9, 26 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by 9, 26 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Tue, 31 Jan 2012 11:54:05 9, 26 -- -0600 9, 26 -- From: Christine Gorman {CGorman-at-hookecollege.com} 9, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 26 -- Date: Tue, 31 Jan 2012 11:54:03 -0600 9, 26 -- Subject: Short Course Announcement: SEM 9, 26 -- Thread-Topic: Short Course Announcement: SEM 9, 26 -- Thread-Index: AczffMnRMWHlMPBXSSarFcypoM3KdQAAuJCQ 9, 26 -- Message-ID: {874B1DB532886444A60A015EE363493F5E5D6BA622-at-mccronemsg07.tmg.mccrone.com} 9, 26 -- Accept-Language: en-US 9, 26 -- Content-Language: en-US 9, 26 -- X-MS-Has-Attach: 9, 26 -- X-MS-TNEF-Correlator: 9, 26 -- x-vipre-scanned: 0634D555002CDB0634D6A2 9, 26 -- x-ninja-pim: Scanned by Ninja 9, 26 -- x-ninja-attachmentfiltering: (no action) 9, 26 -- acceptlanguage: en-US 9, 26 -- Content-Type: text/plain; charset="iso-8859-1" 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q0VHs7fE025979 ==============================End of - Headers==============================
I have a chitin hydrogel that I would like to image in TEM. I did some research and there are reports that standard EM heavy metal stains (osmium, UA, lead citrate) will stain chitin, however, there are also papers that indicate that chitin doesn't pick up these stains. I was wondering if anyone has any experiance staining chitin for TEM.
Ideally, a protocol to stain sections on a grid would be best as I have some un-stained sections (there is iron oxide in the gel which I wanted to visualize without any stain). If this isn't possible then I can process some additional samples.
Thanks very much, Lyle
-- Lyle Gordon Department of Materials Science and Engineering Northwestern University
2220 Campus Drive Evanston, IL 60208
Tel: (847) 491-3584 lgordon-at-u.northwestern.edu
==============================Original Headers============================== 7, 23 -- From lgordon-at-gmail.com Tue Jan 31 14:33:38 2012 7, 23 -- Received: from mail-lpp01m010-f41.google.com (mail-lpp01m010-f41.google.com [209.85.215.41]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q0VKXbnj013643 7, 23 -- for {microscopy-at-microscopy.com} ; Tue, 31 Jan 2012 14:33:38 -0600 7, 23 -- Received: by lamf4 with SMTP id f4so310098lam.0 7, 23 -- for {microscopy-at-microscopy.com} ; Tue, 31 Jan 2012 12:33:37 -0800 (PST) 7, 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 23 -- d=gmail.com; s=gamma; 7, 23 -- h=mime-version:from:date:message-id:subject:to:content-type; 7, 23 -- bh=fAVfgQt8embJHdxesgcDvXPEH/Syq3PdaSq3ZVI3Tog=; 7, 23 -- b=DHSEEyKH90yWDvwCxlJNp3g768nN8qYLe1YuN2MIC5nD+z2LrZ5vOzdcZsBFcfKUEu 7, 23 -- OR5gn2dsEDbfEmflZADE43TZyXqwc+tE3mwwZjl0Z5P/V5i/TPnPAwG957H/HKLcBFEN 7, 23 -- Gu8IHEJN1/W7A4vMnBBufzU7zlZuVw9IfQjTE= 7, 23 -- Received: by 10.112.103.168 with SMTP id fx8mr3454295lbb.14.1328042017233; 7, 23 -- Tue, 31 Jan 2012 12:33:37 -0800 (PST) 7, 23 -- MIME-Version: 1.0 7, 23 -- Received: by 10.112.27.37 with HTTP; Tue, 31 Jan 2012 12:33:17 -0800 (PST) 7, 23 -- From: Lyle Gordon {lgordon-at-gmail.com} 7, 23 -- Date: Tue, 31 Jan 2012 14:33:17 -0600 7, 23 -- Message-ID: {CAFhTNAmT=KfvvLPP42OZkZTXCe07vLuqsz5PkkP_TsrgRZvOFA-at-mail.gmail.com} 7, 23 -- Subject: Staining Chitin for TEM 7, 23 -- To: microscopy-at-microscopy.com 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cmazareanu-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cmazareanu-at-yahoo.com Name: constantin
Title-Subject: [Filtered] Hitachi s-450
Message: Hi all! I looking for spare/defective parts from a Hitachi s-450 SEM, free or at low cost. I am interested mostly for microscope main board or other parts. Thank you for reading my post. Constantin
Hello all, I have following trouble. When starting our Philips CM12 after this night short blackout I've noticed a strange compressed air noise somewhere around the diffusion pump. I cannot find out which valve is responsible for it, because OPCON console cannot be switched to the Vacuum page (pumps and valves scheme) at this time due to a line scan calibration process. Thanking you in advance for any suggestion.
Best regards Oldrich
==============================Original Headers============================== 2, 36 -- From benada-at-biomed.cas.cz Wed Feb 1 02:27:29 2012 2, 36 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 2, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q118RTcU032332 2, 36 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 02:27:29 -0600 2, 36 -- X-ASG-Debug-ID: 1328084846-011d813302a47d20001-4CH8be 2, 36 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id tD7rGRAmenAJ2VFZ (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Wed, 01 Feb 2012 09:27:26 +0100 (CET) 2, 36 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 2, 36 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 2, 36 -- Received: from u117ob.localnet (unknown [147.231.44.252]) 2, 36 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 2, 36 -- (No client certificate requested) 2, 36 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id CC3B72C81AB 2, 36 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 09:27:26 +0100 (CET) 2, 36 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 2, 36 -- Organization: =?utf-8?q?Mikrobiologick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?=, v.v.i. 2, 36 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 2, 36 -- Subject: CM12 trouble 2, 36 -- Date: Wed, 1 Feb 2012 09:27:10 +0100 2, 36 -- X-ASG-Orig-Subj: CM12 trouble 2, 36 -- User-Agent: KMail/1.13.7 (Linux/3.1.0-1-686-pae; KDE/4.6.5; i686; ; ) 2, 36 -- MIME-Version: 1.0 2, 36 -- Content-Type: Text/Plain; 2, 36 -- charset="us-ascii" 2, 36 -- Content-Transfer-Encoding: 7bit 2, 36 -- Message-Id: {201202010927.11087.benada-at-biomed.cas.cz} 2, 36 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 2, 36 -- X-Barracuda-Start-Time: 1328084846 2, 36 -- X-Barracuda-Encrypted: AES256-SHA 2, 36 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 2, 36 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 2, 36 -- X-Barracuda-Spam-Score: 0.00 2, 36 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 tests= 2, 36 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.87278 2, 36 -- Rule breakdown below 2, 36 -- pts rule name description 2, 36 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
I would guess that the problem is coming from the o-rings on the fill and drain caps of the diffusion pump. These hardened with time and the high temperatures that they work under. They take on a triangular cross-section. When the pump cools down, they shrink a fraction, then when re-heated, because they are no longer elastic, they don't seal fully. The o-rings will have quite a short life if you use Viton ones- you can buy black perlast ones for 3x the price of Viton, or Kalrez ones for 4x the price that have better resistance to oil and heat.
Ben
On 01/02/2012 08:34, "benada-at-biomed.cas.cz" {benada-at-biomed.cas.cz} wrote:
} -------------------------------------------------------------------------- } } Hello all, } I have following trouble. When starting our Philips CM12 after this night } short blackout I've noticed a strange compressed air noise somewhere } around } the diffusion pump. I cannot find out which valve is responsible for it, } because } OPCON console cannot be switched to the Vacuum page (pumps and valves } scheme) } at this time due to a line scan calibration process. } Thanking you in advance for any suggestion. } } Best regards Oldrich
==============================Original Headers============================== 10, 30 -- From ben.micklem-at-pharm.ox.ac.uk Wed Feb 1 04:05:51 2012 10, 30 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11A5puR018658 10, 30 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 04:05:51 -0600 10, 30 -- Received: from smtp2.nexus.ox.ac.uk ([163.1.154.136] helo=exht02.ad.oak.ox.ac.uk) 10, 30 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.75) 10, 30 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 10, 30 -- id 1RsX4Y-0003OL-Vm 10, 30 -- for microscopy-at-microscopy.com; Wed, 01 Feb 2012 10:05:50 +0000 10, 30 -- Received: from EXMBX04.ad.oak.ox.ac.uk ([169.254.2.104]) by 10, 30 -- exht02.ad.oak.ox.ac.uk ([163.1.154.53]) with mapi; Wed, 1 Feb 2012 10:05:50 10, 30 -- +0000 10, 30 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 10, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 30 -- Date: Wed, 1 Feb 2012 10:05:50 +0000 10, 30 -- Subject: Re: [Microscopy] CM12 trouble 10, 30 -- Thread-Topic: [Microscopy] CM12 trouble 10, 30 -- Thread-Index: AczgyROpP8nafNyNRv6LFbO0gUWTWA== 10, 30 -- Message-ID: {CB4EBAF8.1C580%ben.micklem-at-pharm.ox.ac.uk} 10, 30 -- In-Reply-To: {201202010834.q118YKVk010652-at-ns.microscopy.com} 10, 30 -- Accept-Language: en-US, en-GB 10, 30 -- Content-Language: en-US 10, 30 -- X-MS-Has-Attach: 10, 30 -- X-MS-TNEF-Correlator: 10, 30 -- user-agent: Microsoft-MacOutlook/14.14.0.111121 10, 30 -- acceptlanguage: en-US, en-GB 10, 30 -- Content-Type: text/plain; charset="us-ascii" 10, 30 -- MIME-Version: 1.0 10, 30 -- Content-Transfer-Encoding: 8bit 10, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q11A5puR018658 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stevebuckingham-at-comcast.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: stevebuckingham-at-comcast.net Name: Steve Buckingham
Organization: Sakti3
Title-Subject: [Filtered] SEM. Extended continuous operation of Hitachi S4500
Message: Hi All, I want to run an extended continuous experiment that images a feature for 24 hours or more. I'm using an Hitachi S4500 cold cathode FESEM and the system trips (beam shut off due to emission drift?) or the it wants a tip flash that stops my experiment. Can anyone tell me if it is possible to run this kind of an experiment on this system and what I need to do to achieve continuous operation of the system over 24 hours or more. Many thanks, Steve
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both marcela.redigolo-at-mail.wvu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
At the present moment, we are reviewing the changes in our electron microscopy facility that were implemented 6 months ago. Also, we are ready to change unsuccessful methods for new ones. For that, I decided to do some benchmarking to check other ideas, models and results in other electron microscopy facilities that could help us improve our ways.
I have a group of topics/questions related to the subject and I would like to discuss them with anyone that would be interested and available. If you can help, please contact me directly through my email (marcela.redigolo at mail.wvu.edu). I appreciate any help.
Thanks for your time!
Kindest regards,
Marcela.
--- Dr. Marcela Redigolo Electron Microscopy Facility WVU Shared Research Facilities West Virginia University
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
realname - Amanda Lever Email - {mailto:lever.amanda1-at-gmail.com} lever.amanda1-at-gmail.com ORGANIZATION - Draper Laboratory EDUCATION - Graduate College LOCATION - Boston, MA SUBJECT_OF_QUESTION - Uranyl Acetate Stain QUESTION - I am staining liposome with 2 % UA on plasma treated Carbon Type-B grids. Is there anything I can add to my UA or grid to reduce aggregation ? Thanks.
==============================Original Headers============================== 4, 24 -- From oshel1pe-at-cmich.edu Wed Feb 1 09:32:24 2012 4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11FWOAv015736 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 09:32:24 -0600 4, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 4, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q11FWLNW032073 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 10:32:24 -0500 4, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 4, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Wed, 1 Feb 4, 24 -- 2012 10:32:22 -0500 4, 24 -- MIME-Version: 1.0 4, 24 -- Message-ID: {a06240804cb4f0b13c22d-at-[141.209.160.249]} 4, 24 -- Date: Wed, 1 Feb 2012 10:32:19 -0500 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 24 -- Subject: How to deaggregate liposomes for TEM? 4, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 24 -- X-Originating-IP: [141.209.160.249] 4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) 4, 24 -- X-Spam-Score: -0.60 () [Hold at 6.00] _L_LLEXCH,RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 4, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 24 -- X-CanItPRO-Stream: default 4, 24 -- X-Canit-Stats-ID: 07GsDwnnK - b6240d7d46e9 - 20120201 4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Dear All, our lab is trying to set up some facilities to prepare bio samples for TEM characterization.However, i have little knowledge on bio sample preparation for TEM. On the list of some equipment purchase, we are getting a centrifuge and a rotator. i would like to ask for advice
1) Is it critical to get a centrifuge with a swing out attachment for the centrifuge tube or a fix inclined angle type would be good enough? 2) I realise that the rotator have different degrees e.g 35, 45 and 55 degree, what is the use of different angle on these rotator?
Cheers, Yee Yan Nanyang Technological University FACTS LAB
==============================Original Headers============================== 4, 35 -- From one_twinklestar-at-yahoo.com.sg Wed Feb 1 11:01:31 2012 4, 35 -- Received: from nm29-vm6.bullet.mail.sg3.yahoo.com (nm29-vm6.bullet.mail.sg3.yahoo.com [106.10.151.165]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q11H1Ts1001662 4, 35 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 11:01:30 -0600 4, 35 -- Received: from [106.10.166.127] by nm29.bullet.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 4, 35 -- Received: from [106.10.151.253] by tm16.bullet.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 4, 35 -- Received: from [127.0.0.1] by omp1002.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 4, 35 -- X-Yahoo-Newman-Property: ymail-3 4, 35 -- X-Yahoo-Newman-Id: 197265.72763.bm-at-omp1002.mail.sg3.yahoo.com 4, 35 -- Received: (qmail 3527 invoked by uid 60001); 1 Feb 2012 17:01:28 -0000 4, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1328115688; bh=pwpaMCQ0Rlv5SdhijeDB4H6RLksIV7mzOl4I0vU4EjI=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=fnM0jRNrvXkl7RVt8QpRAZgsUXD+HXOV8e1efwAFrUfFun9UobZVfrp+30eHiZV4dTWYmVStwvTKTobtkYamprqPKeirVgJNz9zavKCcHu4hmqCo5ddOiXEOC46ONpWrTCURgJT9UD6uiY/pg6w6iExlY96lt27jjm6FU2IKix8= 4, 35 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 35 -- s=s1024; d=yahoo.com.sg; 4, 35 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 4, 35 -- b=GxD8hc8rYEAZ75dmtKPpOsHUhvrxuQadrIpiF8bLy3lmLm+KwVnrKrhtiQweq2MW13XwpIjCDLaEJQ8WtoB69QcNDmBw1UvuZE0mSsleBbQgc60m3p/ZO2wdNg2JlCBu9iRRIJs7bBLmCbPsOGvNp9uK/irbjyhFZsYZv5MPyGs=; 4, 35 -- X-YMail-OSG: T1Ngy.AVM1mv7XBoRwD3QHWhsKEsrVOqvWsbsQhqzndMkJd 4, 35 -- SDjiF12XOPB.fL0TZiAmIRz8IVqTzmBkjo3FsgX4ulMlPt9lbkYTVhoG4R6P 4, 35 -- pwKtfcP620kfgxiXbs141KIyq5EQdLvt09T..Yo1zOFouKcBf3SNax.PBYXQ 4, 35 -- PIgYTW8t_gskBJuvtzgJBF8uuVzN81qyCSnjG3BL3uArpZyk2Wr4.xsn1xt_ 4, 35 -- T9xoorSzXOHHu3srAn1gUZejMg..kMniYVAzThvHztjwALtD6rPWyS0ANT3o 4, 35 -- x8g9qiuSdsx77TK5e_GoWJaFofb2MH.hSaXTVBQhy9_jau649mB.lnXIPslR 4, 35 -- SXzFVv2cghweXEp0Rw_XARYmhoJ_Is5EMbTzyCgrJxyHFzYrSbEpUEY0j2of 4, 35 -- eWYTKELGeHp3AFVDrbQJ8Vgf_d_wjTZ6fni1_FckfWEM55JJR 4, 35 -- Received: from [220.255.188.165] by web77404.mail.sg1.yahoo.com via HTTP; Thu, 02 Feb 2012 01:01:27 SGT 4, 35 -- X-Mailer: YahooMailWebService/0.8.116.331537 4, 35 -- Message-ID: {1328115687.24860.YahooMailNeo-at-web77404.mail.sg1.yahoo.com} 4, 35 -- Date: Thu, 2 Feb 2012 01:01:27 +0800 (SGT) 4, 35 -- From: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 4, 35 -- Reply-To: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 4, 35 -- Subject: Enquires on Purchasing Centrifuge and Rotator 4, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 35 -- MIME-Version: 1.0 4, 35 -- Content-Type: text/plain; charset=utf-8 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q11H1Ts1001662 ==============================End of - Headers==============================
You bring up an interesting question that broadly affects us all, is it possible for a materials lab to successfully cater to biologists?
I see many universities in the US that set up a materials-dedicated EM lab and then invite biologists to use the equipment. While some of the machines on offer are top of the range microscopes, do they adequately serve the needs of the biologist?
Are there biologists on this server who use materials facilities and are successful on getting all their needs addressed? Do all materials labs have ultramicrotomes available, are there cryosectioning, high pressure freezing and freeze substitution machines available? Can 70 nm thin sections be successfully imaged in a TEM operating at 200kVa? Would the people running the facility be willing to turn down the expensive 200kVa machine to 80k?a for biologists to use?
I think the problem goes further than wondering if a centrifuge will be needed for a biologists - of course it will. Similarly with a rotator. A biologist would find this helpful when resin embedding the cell pellets they formed in the centrifuge. But where is the microwave processor for assisting with specimen embedding too?
Personally I think that biological EM will not survive if shared facilities do not provide expert support to biologists, with only limited knowledge of specimen preparation. There is more to what we do than what expensive microscopes provide.
Some facilities are very successful at catering for materials and biological sciences, but others just can't understand why biologists don't come flooding in. The biological scientists are there, but many of them do not know the best way to get results using the EM. These people need to be helped with advice and instruction, and they are also not usually on this listserver.
My advice would be to find a way to get someone on your staff who has expertise in biological EM and use them for the sort of advice you are looking for. At the very least, invite a biologist into your lab to offer advice on a consultative basis.
Best Regards,
Paul Webster House Research Institute 2100 W 3rd St Los Angeles CA 90057 USA
PS Don't forget to register for our Immuno-Gold-Counting Course taking place in April.
-----Original Message----- X-from: one_twinklestar-at-yahoo.com.sg [mailto:one_twinklestar-at-yahoo.com.sg] Sent: Wed 2/1/2012 9:04 AM To: Webster, Paul
Dear All, our lab is trying to set up some facilities to prepare bio samples for TEM characterization.However, i have little knowledge on bio sample preparation for TEM. On the list of some equipment purchase, we are getting a centrifuge and a rotator. i would like to ask for advice
1) Is it critical to get a centrifuge with a swing out attachment for the centrifuge tube or a fix inclined angle type would be good enough? 2) I realise that the rotator have different degrees e.g 35, 45 and 55 degree, what is the use of different angle on these rotator?
Cheers, Yee Yan Nanyang Technological University FACTS LAB
==============================Original Headers============================== 4, 35 -- From one_twinklestar-at-yahoo.com.sg Wed Feb 1 11:01:31 2012 4, 35 -- Received: from nm29-vm6.bullet.mail.sg3.yahoo.com (nm29-vm6.bullet.mail.sg3.yahoo.com [106.10.151.165]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q11H1Ts1001662 4, 35 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 11:01:30 -0600 4, 35 -- Received: from [106.10.166.127] by nm29.bullet.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 4, 35 -- Received: from [106.10.151.253] by tm16.bullet.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 4, 35 -- Received: from [127.0.0.1] by omp1002.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 4, 35 -- X-Yahoo-Newman-Property: ymail-3 4, 35 -- X-Yahoo-Newman-Id: 197265.72763.bm-at-omp1002.mail.sg3.yahoo.com 4, 35 -- Received: (qmail 3527 invoked by uid 60001); 1 Feb 2012 17:01:28 -0000 4, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1328115688; bh=pwpaMCQ0Rlv5SdhijeDB4H6RLksIV7mzOl4I0vU4EjI=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=fnM0jRNrvXkl7RVt8QpRAZgsUXD+HXOV8e1efwAFrUfFun9UobZVfrp+30eHiZV4dTWYmVStwvTKTobtkYamprqPKeirVgJNz9zavKCcHu4hmqCo5ddOiXEOC46ONpWrTCURgJT9UD6uiY/pg6w6iExlY96lt27jjm6FU2IKix8= 4, 35 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 35 -- s=s1024; d=yahoo.com.sg; 4, 35 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 4, 35 -- b=GxD8hc8rYEAZ75dmtKPpOsHUhvrxuQadrIpiF8bLy3lmLm+KwVnrKrhtiQweq2MW13XwpIjCDLaEJQ8WtoB69QcNDmBw1UvuZE0mSsleBbQgc60m3p/ZO2wdNg2JlCBu9iRRIJs7bBLmCbPsOGvNp9uK/irbjyhFZsYZv5MPyGs=; 4, 35 -- X-YMail-OSG: T1Ngy.AVM1mv7XBoRwD3QHWhsKEsrVOqvWsbsQhqzndMkJd 4, 35 -- SDjiF12XOPB.fL0TZiAmIRz8IVqTzmBkjo3FsgX4ulMlPt9lbkYTVhoG4R6P 4, 35 -- pwKtfcP620kfgxiXbs141KIyq5EQdLvt09T..Yo1zOFouKcBf3SNax.PBYXQ 4, 35 -- PIgYTW8t_gskBJuvtzgJBF8uuVzN81qyCSnjG3BL3uArpZyk2Wr4.xsn1xt_ 4, 35 -- T9xoorSzXOHHu3srAn1gUZejMg..kMniYVAzThvHztjwALtD6rPWyS0ANT3o 4, 35 -- x8g9qiuSdsx77TK5e_GoWJaFofb2MH.hSaXTVBQhy9_jau649mB.lnXIPslR 4, 35 -- SXzFVv2cghweXEp0Rw_XARYmhoJ_Is5EMbTzyCgrJxyHFzYrSbEpUEY0j2of 4, 35 -- eWYTKELGeHp3AFVDrbQJ8Vgf_d_wjTZ6fni1_FckfWEM55JJR 4, 35 -- Received: from [220.255.188.165] by web77404.mail.sg1.yahoo.com via HTTP; Thu, 02 Feb 2012 01:01:27 SGT 4, 35 -- X-Mailer: YahooMailWebService/0.8.116.331537 4, 35 -- Message-ID: {1328115687.24860.YahooMailNeo-at-web77404.mail.sg1.yahoo.com} 4, 35 -- Date: Thu, 2 Feb 2012 01:01:27 +0800 (SGT) 4, 35 -- From: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 4, 35 -- Reply-To: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 4, 35 -- Subject: Enquires on Purchasing Centrifuge and Rotator 4, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 35 -- MIME-Version: 1.0 4, 35 -- Content-Type: text/plain; charset=utf-8 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q11H1Ts1001662 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 21 -- From PWebster-at-hei.org Wed Feb 1 11:29:52 2012 24, 21 -- Received: from heimail.hei.org (heimail.hei.org [12.175.189.159]) 24, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11HTpj0018984 24, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 11:29:51 -0600 24, 21 -- Content-class: urn:content-classes:message 24, 21 -- MIME-Version: 1.0 24, 21 -- Content-Type: text/plain; 24, 21 -- charset="iso-8859-1" 24, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 21 -- Subject: RE: [Microscopy] Enquires on Purchasing Centrifuge and Rotator 24, 21 -- Date: Wed, 1 Feb 2012 09:29:50 -0800 24, 21 -- Message-ID: {4B5D9B28C1F49548B4D3C429EE0D32E0390318-at-hi0sml1.hei.org} 24, 21 -- X-MS-Has-Attach: 24, 21 -- X-MS-TNEF-Correlator: 24, 21 -- Thread-Topic: [Microscopy] Enquires on Purchasing Centrifuge and Rotator 24, 21 -- Thread-Index: AczhA45N4hANKL2CTImCANuGi9zwVQAAKWlt 24, 21 -- References: {201202011704.q11H4Qpx006479-at-ns.microscopy.com} 24, 21 -- From: "Webster, Paul" {PWebster-at-hei.org} 24, 21 -- To: {one_twinklestar-at-yahoo.com.sg} , {microscopy-at-microscopy.com} 24, 21 -- Content-Transfer-Encoding: 8bit 24, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q11HTpj0018984 ==============================End of - Headers==============================
since I have googled your facility I now know that the primary target of your Lab-Org will be Materials (http://facts.ntu.edu.sg/about.html ). I would like to congratulate for the interesting facility map which also is displayed at (http://facts.ntu.edu.sg/about_lablayout.html)
Seconding Paul Webster's recent reply (and underline the statements he made) I personally feel that you perhaps have to (then should) add a biological specimen preparation room too... if you would like to attract Biologists to use your facility (nevertheless a compliment for all those machines listed in the map...)
Regarding the secondary questions you were asking, I would like to answer very briefly: Centrifuge(s) (for biological preps preferably equipped with a cooling device) with a "swing out rotor/rotator": would be necessary and IMHO indispensable e.g. for blood centrifugations (esp. for "Buffy Coat"-Preps). All spec.-prep. methods for pelleting cellular biological material (e.g. blood, cell cultures, urinary sediments) will result in a homo- genous pellet/sediment which can be asservated, conserved and finally fixed with respective (chem..)fixative(s) and processed that way into resin. So called "angle-type" centrifuges might serve too, but produce no even, orthogonally layered pellets (especially with regard to the even distribution of "layers" if achievement of such are considered important).
Concerning the different types of "angles": This means that the higher degree of angle the angle velocity and thus the translational forces increase (at the end of the test tube) due to the longer radius of the rotor. So it might well be you'll be able to achieve higher g-forces on your specs with a 55° than with a 35° rotor. Hope this will be of any help with your "decision making"
best regards and greetings to Singapore,
Wolfgang MUSS PhD SALZBURG, AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: one_twinklestar-at-yahoo.com.sg [mailto:one_twinklestar-at-yahoo.com.sg] } Gesendet: Mittwoch, 01. Februar 2012 18:04 } An: Muß Wolfgang } Betreff: [Microscopy] Enquires on Purchasing Centrifuge and Rotator } ----------------------------------------------------------------------- } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Dear All, our lab is trying to set up some facilities to prepare bio } samples for TEM characterization. } However, i have little knowledge on bio sample preparation for TEM. } } On the list of some equipment purchase, we are getting a centrifuge and } a rotator. } i would like to ask for advice } } 1) Is it critical to get a centrifuge with a swing out attachment for } the centrifuge tube or a fix inclined angle type would be good enough? } 2) I realise that the rotator have different degrees e.g 35, 45 and 55 } degree, what is the use of different angle on these rotator? } } Cheers, } Yee Yan } Nanyang Technological University } FACTS LAB } } } ==============================Original } Headers============================== } 4, 35 -- From one_twinklestar-at-yahoo.com.sg Wed Feb 1 11:01:31 2012 } 4, 35 -- Received: from nm29-vm6.bullet.mail.sg3.yahoo.com (nm29- } vm6.bullet.mail.sg3.yahoo.com [106.10.151.165]) } 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } q11H1Ts1001662 } 4, 35 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 11:01:30 - } 0600 } 4, 35 -- Received: from [106.10.166.127] by } nm29.bullet.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 } 4, 35 -- Received: from [106.10.151.253] by } tm16.bullet.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 } 4, 35 -- Received: from [127.0.0.1] by omp1002.mail.sg3.yahoo.com with } NNFMP; 01 Feb 2012 17:01:28 -0000 } 4, 35 -- X-Yahoo-Newman-Property: ymail-3 } 4, 35 -- X-Yahoo-Newman-Id: 197265.72763.bm-at-omp1002.mail.sg3.yahoo.com } 4, 35 -- Received: (qmail 3527 invoked by uid 60001); 1 Feb 2012 } 17:01:28 -0000 } 4, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=yahoo.com.sg; s=s1024; t=1328115688; } bh=pwpaMCQ0Rlv5SdhijeDB4H6RLksIV7mzOl4I0vU4EjI=; h=X-YMail- } OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME- } Version:Content-Type:Content-Transfer-Encoding; } b=fnM0jRNrvXkl7RVt8QpRAZgsUXD+HXOV8e1efwAFrUfFun9UobZVfrp+30eHiZV4dTWYm } VStwvTKTobtkYamprqPKeirVgJNz9zavKCcHu4hmqCo5ddOiXEOC46ONpWrTCURgJT9UD6u } iY/pg6w6iExlY96lt27jjm6FU2IKix8= } 4, 35 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 4, 35 -- s=s1024; d=yahoo.com.sg; } 4, 35 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply- } To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; } 4, 35 -- } b=GxD8hc8rYEAZ75dmtKPpOsHUhvrxuQadrIpiF8bLy3lmLm+KwVnrKrhtiQweq2MW13Xwp } IjCDLaEJQ8WtoB69QcNDmBw1UvuZE0mSsleBbQgc60m3p/ZO2wdNg2JlCBu9iRRIJs7bBLm } CbPsOGvNp9uK/irbjyhFZsYZv5MPyGs=; } 4, 35 -- X-YMail-OSG: T1Ngy.AVM1mv7XBoRwD3QHWhsKEsrVOqvWsbsQhqzndMkJd } 4, 35 -- SDjiF12XOPB.fL0TZiAmIRz8IVqTzmBkjo3FsgX4ulMlPt9lbkYTVhoG4R6P } 4, 35 -- pwKtfcP620kfgxiXbs141KIyq5EQdLvt09T..Yo1zOFouKcBf3SNax.PBYXQ } 4, 35 -- PIgYTW8t_gskBJuvtzgJBF8uuVzN81qyCSnjG3BL3uArpZyk2Wr4.xsn1xt_ } 4, 35 -- T9xoorSzXOHHu3srAn1gUZejMg..kMniYVAzThvHztjwALtD6rPWyS0ANT3o } 4, 35 -- x8g9qiuSdsx77TK5e_GoWJaFofb2MH.hSaXTVBQhy9_jau649mB.lnXIPslR } 4, 35 -- SXzFVv2cghweXEp0Rw_XARYmhoJ_Is5EMbTzyCgrJxyHFzYrSbEpUEY0j2of } 4, 35 -- eWYTKELGeHp3AFVDrbQJ8Vgf_d_wjTZ6fni1_FckfWEM55JJR } 4, 35 -- Received: from [220.255.188.165] by } web77404.mail.sg1.yahoo.com via HTTP; Thu, 02 Feb 2012 01:01:27 SGT } 4, 35 -- X-Mailer: YahooMailWebService/0.8.116.331537 } 4, 35 -- Message-ID: } {1328115687.24860.YahooMailNeo-at-web77404.mail.sg1.yahoo.com} } 4, 35 -- Date: Thu, 2 Feb 2012 01:01:27 +0800 (SGT) } 4, 35 -- From: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} } 4, 35 -- Reply-To: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} } 4, 35 -- Subject: Enquires on Purchasing Centrifuge and Rotator } 4, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 4, 35 -- MIME-Version: 1.0 } 4, 35 -- Content-Type: text/plain; charset=utf-8 } 4, 35 -- Content-Transfer-Encoding: 8bit } 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id q11H1Ts1001662 } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 39 -- From W.Muss-at-salk.at Wed Feb 1 12:16:54 2012 10, 39 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 10, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11IGrUI004127 10, 39 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 12:16:54 -0600 10, 39 -- Received: from localhost (localhost [127.0.0.1]) 10, 39 -- by hermes.salk.at (Postfix) with ESMTP id DF25BC38A1 10, 39 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 19:16:51 +0100 (CET) 10, 39 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 10, 39 -- Received: from hermes.salk.at ([127.0.0.1]) 10, 39 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 10, 39 -- with ESMTP id a+1lFnmraOuH for {microscopy-at-microscopy.com} ; 10, 39 -- Wed, 1 Feb 2012 19:16:51 +0100 (CET) 10, 39 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 10, 39 -- by hermes.salk.at (Postfix) with ESMTP id 83B3CC381E 10, 39 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 19:16:51 +0100 (CET) 10, 39 -- Received: from n1rz122.lksdom21.lks.local ([192.168.101.122]) 10, 39 -- by N2EX199.lks.local with ESMTP; 01 Feb 2012 19:18:04 +0100 10, 39 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.133]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.4675); 10, 39 -- Wed, 1 Feb 2012 19:16:51 +0100 10, 39 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 39 -- Content-class: urn:content-classes:message 10, 39 -- MIME-Version: 1.0 10, 39 -- Content-Type: text/plain; 10, 39 -- charset="iso-8859-1" 10, 39 -- Subject: [Microscopy] Re: Enquires on Purchasing Centrifuge and Rotator 10, 39 -- Date: Wed, 1 Feb 2012 19:16:31 +0100 10, 39 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062F335ED-at-N1RZ116.lksdom21.lks.local} 10, 39 -- In-Reply-To: {201202011704.q11H4M1R006420-at-ns.microscopy.com} 10, 39 -- X-MS-Has-Attach: 10, 39 -- X-MS-TNEF-Correlator: 10, 39 -- Thread-Topic: [Microscopy] Re: Enquires on Purchasing Centrifuge and Rotator 10, 39 -- Thread-Index: AczhA5/uG92mKHgnR9CR7wspJQWfwgABrDIA 10, 39 -- References: {201202011704.q11H4M1R006420-at-ns.microscopy.com} 10, 39 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 10, 39 -- To: {microscopy-at-microscopy.com} 10, 39 -- X-OriginalArrivalTime: 01 Feb 2012 18:16:51.0142 (UTC) FILETIME=[AB491660:01CCE10D] 10, 39 -- X-Scanned-By: SALK-Content-Filter 10, 39 -- Content-Transfer-Encoding: 8bit 10, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q11IGrUI004127 ==============================End of - Headers==============================
Hi Amanda, First I should disclose that I develop products at Dune Sciences, a commercial supplier of functionalized TEM grids for advanced applications. In response to your question restated below, functionalized Carbon grids will improve and simplify the sample preparation of liposomes. The positive charge on the grid disperses the liposomes during deposition and plasma treating is not required as the grids are hydrophilic. You can learn how functionalization chemistry works by going to the following link. http://www.dunesciences.com/grids_CSMART.php
SUBJECT_OF_QUESTION - Uranyl Acetate Stain QUESTION - I am staining liposome with 2 % UA on plasma treated Carbon Type-B grids. Is there anything I can add to my UA or grid to reduce aggregation ? Thanks.
Thanks,
John Miller Dune Sciences, Inc. 541-359-1959 jmiller-at-dunesciences.com
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Wednesday, February 01, 2012 7:41 AM To: jmiller-at-dunesciences.com
**************************************************************************** *********** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************** ************
realname - Amanda Lever Email - {mailto:lever.amanda1-at-gmail.com} lever.amanda1-at-gmail.com ORGANIZATION - Draper Laboratory EDUCATION - Graduate College LOCATION - Boston, MA SUBJECT_OF_QUESTION - Uranyl Acetate Stain QUESTION - I am staining liposome with 2 % UA on plasma treated Carbon Type-B grids. Is there anything I can add to my UA or grid to reduce aggregation ? Thanks.
==============================Original Headers============================== 4, 24 -- From oshel1pe-at-cmich.edu Wed Feb 1 09:32:24 2012 4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11FWOAv015736 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 09:32:24 -0600 4, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 4, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id q11FWLNW032073 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 10:32:24 -0500 4, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 4, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Wed, 1 Feb 4, 24 -- 2012 10:32:22 -0500 4, 24 -- MIME-Version: 1.0 4, 24 -- Message-ID: {a06240804cb4f0b13c22d-at-[141.209.160.249]} 4, 24 -- Date: Wed, 1 Feb 2012 10:32:19 -0500 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 24 -- Subject: How to deaggregate liposomes for TEM? 4, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 24 -- X-Originating-IP: [141.209.160.249] 4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN) 4, 24 -- X-Spam-Score: -0.60 () [Hold at 6.00] _L_LLEXCH,RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 4, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 24 -- X-CanItPRO-Stream: default 4, 24 -- X-Canit-Stats-ID: 07GsDwnnK - b6240d7d46e9 - 20120201 4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 27 -- From jmiller-at-dunesciences.com Wed Feb 1 13:22:24 2012 18, 27 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 18, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11JMORZ022272 18, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 13:22:24 -0600 18, 27 -- Received: by vbip1 with SMTP id p1so1474590vbi.0 18, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 01 Feb 2012 11:22:24 -0800 (PST) 18, 27 -- Received: by 10.52.38.10 with SMTP id c10mr13308880vdk.58.1328124143891; 18, 27 -- Wed, 01 Feb 2012 11:22:23 -0800 (PST) 18, 27 -- Received: from Melange (d181-183.rrp.net. [128.223.181.183]) 18, 27 -- by mx.google.com with ESMTPS id ie6sm23246083vdb.5.2012.02.01.11.22.22 18, 27 -- (version=SSLv3 cipher=OTHER); 18, 27 -- Wed, 01 Feb 2012 11:22:23 -0800 (PST) 18, 27 -- Message-ID: {4f2990ef.066f340a.703e.3221-at-mx.google.com} 18, 27 -- Reply-To: {jmiller-at-dunesciences.com} 18, 27 -- From: "John M. Miller" {jmiller-at-dunesciences.com} 18, 27 -- To: {Microscopy-at-microscopy.com} , {lever.amanda1-at-gmail.com} 18, 27 -- In-Reply-To: {201202011540.q11FeV2V028207-at-ns.microscopy.com} 18, 27 -- Subject: RE: [Microscopy] How to deaggregate liposomes for TEM? 18, 27 -- Date: Wed, 1 Feb 2012 11:22:22 -0800 18, 27 -- Organization: Dune Sciences 18, 27 -- MIME-Version: 1.0 18, 27 -- Content-Type: text/plain; 18, 27 -- charset="us-ascii" 18, 27 -- Content-Transfer-Encoding: 7bit 18, 27 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 18, 27 -- Thread-Index: Aczg99V6kpK25vQoS22D2xxlM7m4rgAHjfyA 18, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.1.7601.17609 ==============================End of - Headers==============================
We have a 120kV FEI Tecnai 12 Twin TEM which was chosen to serve multiple purpose projects. On installation the scope was rated at 2nm. We have stereo-imaged 500nm sections, 70nm sections of epoxy-embedded silica gel impregnated with Cobalt oxide deposits, and nano-particulates on C-coated grids. For a relatively non-analytic, vanilla, 120kV instrument, I believe that is not bad. I routinely view thin sections (60-80nm) of biological tissue post-fixed with osmium and permit the 1k Gatan to add the contrast which is usually quite satisfactory. The vacuum is usually in the middle of 10-8 T which is also more than satisfactory. We did not spec a 'bio' lens configuration, because we wanted to be flexible. We have a lab equipped as can be seen on our web site.
Most of our users are not biologists, but we can certainly do biologic specimens without any problem. The only difference I have imposed on the use of the TEM is that all biological specimens and those nano particles produced by wet chemistry and not presented in light alcohols are used with the LN2 coldfinger to prevent general contamination in the stage area.
Short of the accessories for vitreous quenching, the biggest difference will lie with the biological core facility that cannot be used by materials scientists - no matter what the size/expense.
Finally, having said all of the above, there are choices in instruments, because there is variety of need. One can stretch one's flexibility so far that it excludes the need one was supposed to address in the first place. A core facility with a Titan and a 120kV Bio-Twin would serve both material and biological investigators. Under what conditions would a biologist need wave-length resolution and not, in truth, be working on bio-materials.
As an example, if I had the money and I wanted to purchase a Titan with 10 years of service, I might spend it and have the package delivered to someone who has a discernible need. I, even when I try with diligence, cannot uncover a need/use for myself. So, I would not recommend such an acquisition, even if it were offered, if I could not worry up a need to have it. Then, of course, the ceiling here is not nearly high enough, and even though on bedrock, and lacking subways, there are still trucks and busses, AND, where I now need only two rooms for the TEM, I would need at least three for a Titan, and perhaps for a 200kV with no observation chamber as well.
The Dean of a college has a materials-oriented core facility for which s/he is responsible (services, maintenance, and cost recovery). S/he charges the Deans of other colleges whose Faculty use the facility. The President owns the Colleges and the cores, and spends money on an accounting scheme which issues bills for use to Departments and Individuals who have external funding. There is no mechanism to charge for undergraduate courses, which, thus, do not usually fit into such a scheme. So the core is limited to research use, and many bright students choose business and finance.
Cheers,
Fred Monson
P.S. The day is almost over, and I look forward to the continuing mechanical and optical attention I am paying to one of my antique Leitz 'Research' Microscopes. I could NEVER do that with a Titan, nor even with our Tecnai. How sad.... I, not a Tinker, Tailor, nor a Soldier be, but merely a mechanic - just like me Dad. God bless 'im!
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
If one looks long enough, one may actually see something frozen in time that flashes one's mind to a new state. 1. The day that I asked myself how the hydrogen atom's mass could actually be: 1.0008. 2. The day, while looking at a slide of some tissue, I saw a leukocyte that had been caught sneaking in or out of a capillary: http://www.annualreviews.org/doi/abs/10.1146/annurev-pathol-011110-130224
-----Original Message----- X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org] Sent: Wednesday, February 01, 2012 12:36 PM To: Monson, Frederick
Dear Yee Yan,
You bring up an interesting question that broadly affects us all, is it possible for a materials lab to successfully cater to biologists?
I see many universities in the US that set up a materials-dedicated EM lab and then invite biologists to use the equipment. While some of the machines on offer are top of the range microscopes, do they adequately serve the needs of the biologist?
Are there biologists on this server who use materials facilities and are successful on getting all their needs addressed? Do all materials labs have ultramicrotomes available, are there cryosectioning, high pressure freezing and freeze substitution machines available? Can 70 nm thin sections be successfully imaged in a TEM operating at 200kVa? Would the people running the facility be willing to turn down the expensive 200kVa machine to 80k?a for biologists to use?
I think the problem goes further than wondering if a centrifuge will be needed for a biologists - of course it will. Similarly with a rotator. A biologist would find this helpful when resin embedding the cell pellets they formed in the centrifuge. But where is the microwave processor for assisting with specimen embedding too?
Personally I think that biological EM will not survive if shared facilities do not provide expert support to biologists, with only limited knowledge of specimen preparation. There is more to what we do than what expensive microscopes provide.
Some facilities are very successful at catering for materials and biological sciences, but others just can't understand why biologists don't come flooding in. The biological scientists are there, but many of them do not know the best way to get results using the EM. These people need to be helped with advice and instruction, and they are also not usually on this listserver.
My advice would be to find a way to get someone on your staff who has expertise in biological EM and use them for the sort of advice you are looking for. At the very least, invite a biologist into your lab to offer advice on a consultative basis.
Best Regards,
Paul Webster House Research Institute 2100 W 3rd St Los Angeles CA 90057 USA
PS Don't forget to register for our Immuno-Gold-Counting Course taking place in April.
-----Original Message----- X-from: one_twinklestar-at-yahoo.com.sg [mailto:one_twinklestar-at-yahoo.com.sg] Sent: Wed 2/1/2012 9:04 AM To: Webster, Paul
Dear All, our lab is trying to set up some facilities to prepare bio samples for TEM characterization.However, i have little knowledge on bio sample preparation for TEM. On the list of some equipment purchase, we are getting a centrifuge and a rotator. i would like to ask for advice
1) Is it critical to get a centrifuge with a swing out attachment for the centrifuge tube or a fix inclined angle type would be good enough? 2) I realise that the rotator have different degrees e.g 35, 45 and 55 degree, what is the use of different angle on these rotator?
Cheers, Yee Yan Nanyang Technological University FACTS LAB
==============================Original Headers============================== 4, 35 -- From one_twinklestar-at-yahoo.com.sg Wed Feb 1 11:01:31 2012 4, 35 -- Received: from nm29-vm6.bullet.mail.sg3.yahoo.com (nm29-vm6.bullet.mail.sg3.yahoo.com [106.10.151.165]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q11H1Ts1001662 4, 35 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 11:01:30 -0600 4, 35 -- Received: from [106.10.166.127] by nm29.bullet.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 4, 35 -- Received: from [106.10.151.253] by tm16.bullet.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 4, 35 -- Received: from [127.0.0.1] by omp1002.mail.sg3.yahoo.com with NNFMP; 01 Feb 2012 17:01:28 -0000 4, 35 -- X-Yahoo-Newman-Property: ymail-3 4, 35 -- X-Yahoo-Newman-Id: 197265.72763.bm-at-omp1002.mail.sg3.yahoo.com 4, 35 -- Received: (qmail 3527 invoked by uid 60001); 1 Feb 2012 17:01:28 -0000 4, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1328115688; bh=pwpaMCQ0Rlv5SdhijeDB4H6RLksIV7mzOl4I0vU4EjI=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=fnM0jRNrvXkl7RVt8QpRAZgsUXD+HXOV8e1efwAFrUfFun9UobZVfrp+30eHiZV4dTWYmVStwvTKTobtkYamprqPKeirVgJNz9zavKCcHu4hmqCo5ddOiXEOC46ONpWrTCURgJT9UD6uiY/pg6w6iExlY96lt27jjm6FU2IKix8= 4, 35 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 35 -- s=s1024; d=yahoo.com.sg; 4, 35 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 4, 35 -- b=GxD8hc8rYEAZ75dmtKPpOsHUhvrxuQadrIpiF8bLy3lmLm+KwVnrKrhtiQweq2MW13XwpIjCDLaEJQ8WtoB69QcNDmBw1UvuZE0mSsleBbQgc60m3p/ZO2wdNg2JlCBu9iRRIJs7bBLmCbPsOGvNp9uK/irbjyhFZsYZv5MPyGs=; 4, 35 -- X-YMail-OSG: T1Ngy.AVM1mv7XBoRwD3QHWhsKEsrVOqvWsbsQhqzndMkJd 4, 35 -- SDjiF12XOPB.fL0TZiAmIRz8IVqTzmBkjo3FsgX4ulMlPt9lbkYTVhoG4R6P 4, 35 -- pwKtfcP620kfgxiXbs141KIyq5EQdLvt09T..Yo1zOFouKcBf3SNax.PBYXQ 4, 35 -- PIgYTW8t_gskBJuvtzgJBF8uuVzN81qyCSnjG3BL3uArpZyk2Wr4.xsn1xt_ 4, 35 -- T9xoorSzXOHHu3srAn1gUZejMg..kMniYVAzThvHztjwALtD6rPWyS0ANT3o 4, 35 -- x8g9qiuSdsx77TK5e_GoWJaFofb2MH.hSaXTVBQhy9_jau649mB.lnXIPslR 4, 35 -- SXzFVv2cghweXEp0Rw_XARYmhoJ_Is5EMbTzyCgrJxyHFzYrSbEpUEY0j2of 4, 35 -- eWYTKELGeHp3AFVDrbQJ8Vgf_d_wjTZ6fni1_FckfWEM55JJR 4, 35 -- Received: from [220.255.188.165] by web77404.mail.sg1.yahoo.com via HTTP; Thu, 02 Feb 2012 01:01:27 SGT 4, 35 -- X-Mailer: YahooMailWebService/0.8.116.331537 4, 35 -- Message-ID: {1328115687.24860.YahooMailNeo-at-web77404.mail.sg1.yahoo.com} 4, 35 -- Date: Thu, 2 Feb 2012 01:01:27 +0800 (SGT) 4, 35 -- From: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 4, 35 -- Reply-To: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 4, 35 -- Subject: Enquires on Purchasing Centrifuge and Rotator 4, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 35 -- MIME-Version: 1.0 4, 35 -- Content-Type: text/plain; charset=utf-8 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q11H1Ts1001662 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 21 -- From PWebster-at-hei.org Wed Feb 1 11:29:52 2012 24, 21 -- Received: from heimail.hei.org (heimail.hei.org [12.175.189.159]) 24, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11HTpj0018984 24, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 11:29:51 -0600 24, 21 -- Content-class: urn:content-classes:message 24, 21 -- MIME-Version: 1.0 24, 21 -- Content-Type: text/plain; 24, 21 -- charset="iso-8859-1" 24, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 21 -- Subject: RE: [Microscopy] Enquires on Purchasing Centrifuge and Rotator 24, 21 -- Date: Wed, 1 Feb 2012 09:29:50 -0800 24, 21 -- Message-ID: {4B5D9B28C1F49548B4D3C429EE0D32E0390318-at-hi0sml1.hei.org} 24, 21 -- X-MS-Has-Attach: 24, 21 -- X-MS-TNEF-Correlator: 24, 21 -- Thread-Topic: [Microscopy] Enquires on Purchasing Centrifuge and Rotator 24, 21 -- Thread-Index: AczhA45N4hANKL2CTImCANuGi9zwVQAAKWlt 24, 21 -- References: {201202011704.q11H4Qpx006479-at-ns.microscopy.com} 24, 21 -- From: "Webster, Paul" {PWebster-at-hei.org} 24, 21 -- To: {one_twinklestar-at-yahoo.com.sg} , {microscopy-at-microscopy.com} 24, 21 -- Content-Transfer-Encoding: 8bit 24, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q11HTpj0018984 ==============================End of - Headers==============================
==============================Original Headers============================== 42, 37 -- From FMonson-at-wcupa.edu Wed Feb 1 14:06:40 2012 42, 37 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 42, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11K6eba007317 42, 37 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 14:06:40 -0600 42, 37 -- X-ASG-Debug-ID: 1328126799-0378c357f2e0350001-4CH8be 42, 37 -- Received: from WCU-XCH-08.PASSHE.LCL (wcu-xch-08.wcupa.edu [144.26.0.92]) by MX02.WCUPA.EDU with ESMTP id qdSU9Rl8qN87qEXf (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Wed, 01 Feb 2012 15:06:39 -0500 (EST) 42, 37 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 42, 37 -- X-Barracuda-Apparent-Source-IP: 144.26.0.92 42, 37 -- X-ASG-Whitelist: Client 42, 37 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 42, 37 -- WCU-XCH-08.PASSHE.LCL ([fe80::7048:475f:4ea9:edce%15]) with mapi id 42, 37 -- 14.01.0270.001; Wed, 1 Feb 2012 15:06:39 -0500 42, 37 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 42, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 42, 37 -- CC: "PWebster-at-hei.org" {PWebster-at-hei.org} 42, 37 -- Subject: RE: [Microscopy] RE: Enquires on Purchasing Centrifuge and Rotator 42, 37 -- Thread-Topic: [Microscopy] RE: Enquires on Purchasing Centrifuge and Rotator 42, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: Enquires on Purchasing Centrifuge and Rotator 42, 37 -- Thread-Index: AQHM4QgFDbVyAmYtokqqLbZqga29D5YoX5PA 42, 37 -- Date: Wed, 1 Feb 2012 20:06:38 +0000 42, 37 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C130FFE-at-WCU-XCH-03.PASSHE.LCL} 42, 37 -- References: {201202011736.q11HaNu5031039-at-ns.microscopy.com} 42, 37 -- In-Reply-To: {201202011736.q11HaNu5031039-at-ns.microscopy.com} 42, 37 -- Accept-Language: en-US 42, 37 -- Content-Language: en-US 42, 37 -- X-MS-Has-Attach: 42, 37 -- X-MS-TNEF-Correlator: 42, 37 -- x-originating-ip: [10.28.57.79] 42, 37 -- Content-Type: text/plain; charset="us-ascii" 42, 37 -- MIME-Version: 1.0 42, 37 -- X-Barracuda-Connect: wcu-xch-08.wcupa.edu[144.26.0.92] 42, 37 -- X-Barracuda-Start-Time: 1328126799 42, 37 -- X-Barracuda-Encrypted: AES128-SHA 42, 37 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 42, 37 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 42, 37 -- Content-Transfer-Encoding: 8bit 42, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q11K6eba007317 ==============================End of - Headers==============================
I use LR white almost exclusively for immunolabeling in plant tissue and have very good luck with it. The protocol that I use I got from Kevin Vaughn, so I would suggest that you check out a few of his papers. Some of his earlier work was on immunolabeling various proteins in chloroplasts, so they may be especially relevant to you, though the later papers may have slightly more up-to-date protocols.
If you have any trouble finding his papers or need more detail on any of the steps of the protocol, just let me know.
Andy
_____________________ Andrew J Bowling, PhD Discovery Research Dow AgroSciences 9330 Zionsville Rd Indianapolis, IN 46268 317-337-3878
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, January 27, 2012 7:19 PM To: Bowling, Andrew (AJ)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stempies-at-muohio.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Resins for immunocytochemistry
Message: Dear Listserv,
I am looking at immunogold labeling proteins in Arabidopsis chloroplasts for TEM and was wondering if anyone on the Listserv had experience with the differences in immunocytochemistry resins. I'm interested in knowing how well Unicryl, LR White, and LR Gold have performed in other people's experience with immunogold labeling.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both marcela.redigolo-at-mail.wvu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Thanks!!! [Benchmarking electron microscopy facility]
Message: Dear all:
I just want to thank you for the replies I got. I'm contacting each one individually. Thanks again for the replies so far. Marcela.
----
Email: marcela.redigolo-at-mail.wvu.edu Name: Marcela Redigolo Organization: WVU Title-Subject: [Filtered] Benchmarking Message: Dear all: At the present moment, we are reviewing the changes in our electron microscopy facility that were implemented 6 months ago. Also, we are ready to change unsuccessful methods for new ones. For that, I decided to do some benchmarking to check other ideas, models and results in other electron microscopy facilities that could help us improve our ways. I have a group of topics/questions related to the subject and I would like to discuss them with anyone that would be interested and available. If you can help, please contact me directly through my email (marcela.redigolo at mail.wvu.edu). I appreciate any help. Thanks for your time! Kindest regards, Marcela. --- Dr. Marcela Redigolo Electron Microscopy Facility WVU Shared Research Facilities West Virginia University
Hi Lyle and all, Try the protocol in Lingle, W.L. (1989). Enhanced Staining of the Basidiomycete Panellus stypticus Prepared for TEM by Freeze Substitution. Cryotogamic Botany 1, 236-242. Wilma did a modification of the Thiery stain and this method stains chitin nicely. I couldn't find the journal article on-line but I can snail mail a copy to you if you'd like it. best, Beth
==============================Original Headers============================== 2, 20 -- From beth-at-plantbio.uga.edu Wed Feb 1 15:35:04 2012 2, 20 -- Received: from dogwood.plantbio.uga.edu (pbmail.plantbio.uga.edu [128.192.26.3]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11LZ4C1026946 2, 20 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 15:35:04 -0600 2, 20 -- X-Footer: cGxhbnRiaW8udWdhLmVkdQ== 2, 20 -- Received: from [128.192.26.69] ([128.192.26.69]) 2, 20 -- (authenticated user beth-at-plantbio.uga.edu) 2, 20 -- by dogwood.plantbio.uga.edu 2, 20 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)); 2, 20 -- Wed, 1 Feb 2012 16:35:03 -0500 2, 20 -- Message-Id: {BE4BF3C9-2F9A-41B6-B19A-F2111900188C-at-plantbio.uga.edu} 2, 20 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 2, 20 -- To: microscopy microscopy {microscopy-at-microscopy.com} 2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 20 -- Content-Transfer-Encoding: 7bit 2, 20 -- Mime-Version: 1.0 (Apple Message framework v936) 2, 20 -- Subject: staining chitin 2, 20 -- Date: Wed, 1 Feb 2012 16:35:03 -0500 2, 20 -- Cc: lgordon-at-u.northwestern.edu 2, 20 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Just a thought - the fluorescent lectins are very good at binding specifically to chitin, e.g. fluorescent wheat germ agglutinin. Would a gold-tagged one work in TEM? I don't know if they exist, but it would be very straightforward to do the staining. cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 M 61 2 420 972 028 E rosemary.white-at-csiro.au
On 2/02/12 8:40 AM, "beth-at-plantbio.uga.edu" {beth-at-plantbio.uga.edu} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 31 -- From prvs=371557c51=Rosemary.White-at-csiro.au Wed Feb 1 16:11:27 2012 10, 31 -- Received: from vic-MTAout3.csiro.au (vic-MTAout3.csiro.au [150.229.64.39]) 10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11MBQNo012044 10, 31 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 16:11:27 -0600 10, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 10, 31 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 10, 31 -- s=email; t=1328134287; x=1359670287; 10, 31 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: 10, 31 -- content-transfer-encoding; 10, 31 -- bh=7CfGFrujXlOULVH/DloEj+Sx2rq901bEWq0e6WuOxNc=; 10, 31 -- b=YCgxO69K+AxHpBegsl4gm5Olnpdcs02oqWSLhs+cmyXuIQZ0Gs+B7c1R 10, 31 -- xsjvN/uYK/QBhq6yZTRQ9vysZ+lLxNquA4xRyUi8J89H4nubzrcBpk717 10, 31 -- UGpUMxGH93ww9LQ; 10, 31 -- X-IronPort-AV: E=Sophos;i="4.73,341,1325422800"; 10, 31 -- d="scan'208";a="76920296" 10, 31 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 10, 31 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 02 Feb 2012 09:11:19 +1100 10, 31 -- Received: from [152.83.195.117] (152.83.195.117) by 10, 31 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server 10, 31 -- (TLS) id 8.3.213.0; Thu, 2 Feb 2012 09:11:19 +1100 10, 31 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 10, 31 -- Date: Thu, 2 Feb 2012 09:11:17 +1100 10, 31 -- Subject: Re: [Microscopy] staining chitin 10, 31 -- From: Rosemary White {rosemary.white-at-csiro.au} 10, 31 -- To: {microscopy-at-microscopy.com} 10, 31 -- Message-ID: {CB500303.72AD7%rosemary.white-at-csiro.au} 10, 31 -- Thread-Topic: [Microscopy] staining chitin 10, 31 -- In-Reply-To: {201202012140.q11LewTb002250-at-ns.microscopy.com} 10, 31 -- MIME-Version: 1.0 10, 31 -- Content-Type: text/plain; charset="US-ASCII" 10, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Good idea with the lectins. However, I would not use lectins directly coupled to gold particles. Instead, for EM labeling I would probably apply unbound lectin to the sections and then specific anti-lectin antibodies followed by the gold probe. Then there would be no concern about the gold particles disassociating from the lectin during storage.
Of course, if going the antibody route, why not use anti-chitin antibodies?
Regards
Paul.
Paul Webster, Ph.D. House Research Institute 2100 West Third Street Los Angeles CA 90057 (213) 273 8026
On 2/1/12 2:13 PM, "rosemary.white-at-csiro.au" {rosemary.white-at-csiro.au} wrote:
} microscopy-at-microscopy.com
==============================Original Headers============================== 14, 19 -- From PWebster-at-hei.org Wed Feb 1 16:25:09 2012 14, 19 -- Received: from heimail.hei.org (heimail.hei.org [12.175.189.159]) 14, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q11MP8Cq029148 14, 19 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2012 16:25:09 -0600 14, 19 -- Received: from 10.10.42.25 ([10.10.42.25]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 14, 19 -- Wed, 1 Feb 2012 22:25:08 +0000 14, 19 -- User-Agent: Microsoft-Entourage/12.27.0.100910 14, 19 -- Date: Wed, 01 Feb 2012 14:25:04 -0800 14, 19 -- Subject: Re: [Microscopy] Re: staining chitin 14, 19 -- From: "Webster, Paul" {pwebster-at-hei.org} 14, 19 -- To: {rosemary.white-at-csiro.au} , {microscopy-at-microscopy.com} 14, 19 -- Message-ID: {CB4EFBC0.18AB7%pwebster-at-hei.org} 14, 19 -- Thread-Topic: [Microscopy] Re: staining chitin 14, 19 -- Thread-Index: AczhMFgeFRV9zPMkT6msokN23Qx22Q== 14, 19 -- In-Reply-To: {201202012213.q11MDXjP016844-at-ns.microscopy.com} 14, 19 -- Mime-version: 1.0 14, 19 -- Content-type: text/plain; 14, 19 -- charset="US-ASCII" 14, 19 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both marcela.redigolo-at-mail.wvu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Update - Benchmarking EM Facility
Message: Dear all:
I am receiving valuable information and want to thank you all for your help. Also, many people are asking me to compile the information and post to the list a description of my findings, conclusive or not, that might be helpful for other managers of EM Facilities around. I will try to highlight interesting ideas, similarities and differences. But please, keep in mind, my reference (my base) is our shared facility in WVU. I know there are many facilities out there and in this list that are way bigger than ours. Still I appreciate all contribution. It has been really valuable the messages I'm receiving. If you want to contribute, please answer directly to my email so we won't crowd the listserver here. Thanks again,
Marcela.
---- Dr. Marcela Redigolo Electron Microscopy Facility WVU Shared Research Facilities West Virginia University
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both korinek-at-mcmaster.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: korinek-at-mcmaster.ca Name: Andreas Korinek
Organization: McMaster University
Title-Subject: [Filtered] TEM Lithium Detection By EELs Analysis
Message: I want to detect Lithium in a FIB section at 300 kV. The FIB section is approximately 0.8 MFP thick. I'm using a FEI Titan microscope at 300 kV, equipped with a Tridium GIF. I know that there is a Li signal at 55 eV, I can detect some signal there in my section, the problem is thst I can also see this signal in the protective layer of the section, probably due to phonons. Can anyone give me hints on how to improve the Li signal? How much thinning would be needed to get a good peak in this region?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both leo.erich-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: leo.erich-at-yahoo.com Name: Leo
Title-Subject: [Filtered] EELS quantification error and spectra normalization Message: Dear All
I would like to know :
1) how I can reasonably determine the error bar when I perform spectrum-image (EELS line scan ) measurement across the interface or grain boundary ? 2)how to perform EELS spectra normalization in Digital Micrograph ? I would appreciate very much to have your opinion.
Best,
Leo Login Host: 193.170.20.79 ---------------------------------------------------------------------------
==============================Original Headers============================== 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 2 13:42:44 2012 10, 25 -- Received: from znl.com ([206.69.208.20]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q12JgiV8025947 10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:42:44 -0600 10, 25 -- Received: from localhost (localhost [127.0.0.1]) 10, 25 -- by znl.com (Postfix) with ESMTP id D8936484733 10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:42:44 -0600 (CST) 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 25 -- Received: from znl.com ([127.0.0.1]) 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 25 -- with ESMTP id DQaY7ru8xiyv for {microscopy-at-microscopy.com} ; 10, 25 -- Thu, 2 Feb 2012 13:42:44 -0600 (CST) 10, 25 -- Received: from NestorPwrBk.local (dsl-58-6-4-117.wa.westnet.com.au [58.6.4.117]) 10, 25 -- by znl.com (Postfix) with ESMTPA id F35D9484728 10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:42:43 -0600 (CST) 10, 25 -- Message-ID: {4F2AE731.103-at-microscopy.com} 10, 25 -- Date: Fri, 03 Feb 2012 03:42:41 +0800 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 25 -- Reply-To: leo.erich-at-yahoo.com 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 25 -- MIME-Version: 1.0 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 25 -- Subject: viaWWW:EELS quantification error and spectra normalization 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pscallio-at-dal.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pscallio-at-dal.ca Name: Patricia Scallion
Organization: IRM at Dalhousie University
Title-Subject: [Filtered] SDD - standby or operate mode
Message: We have a new SDD Oxford detector on our S-4700. I was wondering if most owners of these systems keep them in Operate mode, or only cool them down when users are scheduled. Are there any issues that may be encountered? Login Host: 129.173.116.162 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 2 13:43:12 2012 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q12JhCXZ027491 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:43:12 -0600 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 31C1148473F 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:43:12 -0600 (CST) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id VhEdk7vFwuhh for {microscopy-at-microscopy.com} ; 7, 25 -- Thu, 2 Feb 2012 13:43:11 -0600 (CST) 7, 25 -- Received: from NestorPwrBk.local (dsl-58-6-4-117.wa.westnet.com.au [58.6.4.117]) 7, 25 -- by znl.com (Postfix) with ESMTPA id 2E7C6484734 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:43:10 -0600 (CST) 7, 25 -- Message-ID: {4F2AE74D.7000106-at-microscopy.com} 7, 25 -- Date: Fri, 03 Feb 2012 03:43:09 +0800 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: pscallio-at-dal.ca 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:SDD - standby or operate mode 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both aphill48-at-uthsc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: University of Tennessee Health Science Center
Title-Subject: [Filtered] Postdoctoral Position - University of Tennessee Health Science Center
Message: Postdoctoral positions: Several postdoctoral positions are immediately available to study the role of GPCR agonists, lipoxygenases and chemokines in angiogenesis and vascular wall remodeling. Experience with animal models of atherosclerosis, protein-protein interactions, and/or molecular biology is highly desirable. Based on experience competitive salaries are offered. Interested and highly motivated candidates with PhD, MD or MD/PhD degree should send curriculum vitae and three letters of references to: Professor GN Rao, Department of Physiology, University of Tennessee Health Science Center, 894 Union Avenue, Memphis, TN 38163. E-mail: rgadipar-at-uthsc.edu. The University of Tennessee is an EEO/AA Title VI/Title IX/Section 504/ADA/ADEA institution in the provision of its education and employment programs and services. Login Host: 128.169.2.153 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 26 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 2 13:43:41 2012 7, 26 -- Received: from znl.com ([206.69.208.20]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q12Jhftt029364 7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:43:41 -0600 7, 26 -- Received: from localhost (localhost [127.0.0.1]) 7, 26 -- by znl.com (Postfix) with ESMTP id 4744548474E 7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:43:41 -0600 (CST) 7, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 26 -- Received: from znl.com ([127.0.0.1]) 7, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 26 -- with ESMTP id bcUN7yTIh-WI for {microscopy-at-microscopy.com} ; 7, 26 -- Thu, 2 Feb 2012 13:43:41 -0600 (CST) 7, 26 -- Received: from NestorPwrBk.local (dsl-58-6-4-117.wa.westnet.com.au [58.6.4.117]) 7, 26 -- by znl.com (Postfix) with ESMTPA id EFDD7484741 7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:43:39 -0600 (CST) 7, 26 -- Message-ID: {4F2AE769.9010209-at-microscopy.com} 7, 26 -- Date: Fri, 03 Feb 2012 03:43:37 +0800 7, 26 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 26 -- Reply-To: aphill48-at-uthsc.edu 7, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 26 -- MIME-Version: 1.0 7, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 26 -- Subject: viaWWW:Postdoctoral Position - University of Tennessee Health Science 7, 26 -- Center 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
It's Friday and if the prospect of a week-end isn't enough, Wired has come through. No, they're not all photographs. The article is about Science Visualizations and some great images. I'm disappointed there's no fusion preps in PLM but the cucumber skin barbs make up for it. Here's the link.
One can't help wonder what the folks at Wired think of the sudden influx of web traffic for specific article.
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 8, 25 -- From frank_karl-at-ardl.com Fri Feb 3 08:23:07 2012 8, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13EN67u012848 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 08:23:07 -0600 8, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 8, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 3 Feb 8, 25 -- 2012 09:23:05 -0500 8, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 8, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 8, 25 -- Date: Fri, 3 Feb 2012 09:23:04 -0500 8, 25 -- Subject: more wired microscopy 8, 25 -- Thread-Topic: more wired microscopy 8, 25 -- Thread-Index: Aczif1eQt2LTUX68QIiPtRHbYYXMcA== 8, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077015845420C-at-exchange2k7.ad.ardl.com} 8, 25 -- Accept-Language: en-US 8, 25 -- Content-Language: en-US 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- acceptlanguage: en-US 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q13EN67u012848 ==============================End of - Headers==============================
I posed the same question to my Oxford service engineer and he told me that they recommend leaving the detector in operate mode. I haven't been able to detect any adverse effects in detector performance.
Bryan
On Thu, Feb 2, 2012 at 1:53 PM, {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both pscallio-at-dal.ca as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: pscallio-at-dal.ca Name: Patricia Scallion } } Organization: IRM at Dalhousie University } } Title-Subject: [Filtered] SDD - standby or operate mode } } Message: We have a new SDD Oxford detector on our S-4700. I was } wondering if most owners of these systems keep them in Operate mode, or } only cool them down when users are scheduled. } Are there any issues that may be encountered? } Login Host: 129.173.116.162 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 2 13:43:12 2012 } 7, 25 -- Received: from znl.com ([206.69.208.20]) } 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q12JhCXZ027491 } 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:43:12 -0600 } 7, 25 -- Received: from localhost (localhost [127.0.0.1]) } 7, 25 -- by znl.com (Postfix) with ESMTP id 31C1148473F } 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:43:12 -0600 (CST) } 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 7, 25 -- Received: from znl.com ([127.0.0.1]) } 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 7, 25 -- with ESMTP id VhEdk7vFwuhh for {microscopy-at-microscopy.com} ; } 7, 25 -- Thu, 2 Feb 2012 13:43:11 -0600 (CST) } 7, 25 -- Received: from NestorPwrBk.local (dsl-58-6-4-117.wa.westnet.com.au [58.6.4.117]) } 7, 25 -- by znl.com (Postfix) with ESMTPA id 2E7C6484734 } 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 2 Feb 2012 13:43:10 -0600 (CST) } 7, 25 -- Message-ID: {4F2AE74D.7000106-at-microscopy.com} } 7, 25 -- Date: Fri, 03 Feb 2012 03:43:09 +0800 } 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 7, 25 -- Reply-To: pscallio-at-dal.ca } 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 7, 25 -- MIME-Version: 1.0 } 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 7, 25 -- Subject: viaWWW:SDD - standby or operate mode } 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
-- _________________________________________________________________ Bryan R. Bandli
Research Instrumentation Laboratory Manager University of Minnesota, Duluth 229 Heller Hall 1114 Kirby Dr. Duluth, MN 55812 218-726-7362 ==================================================================
==============================Original Headers============================== 9, 35 -- From bbandli-at-d.umn.edu Fri Feb 3 08:30:37 2012 9, 35 -- Received: from mx1.d.umn.edu (mx1.d.umn.edu [131.212.109.15]) 9, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13EUb7d021792 9, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 08:30:37 -0600 9, 35 -- Received: from mxr1.d.umn.edu (mxr1.d.umn.edu [131.212.109.92]) 9, 35 -- by mx1.d.umn.edu (8.13.8/8.13.8) with ESMTP id q13ES5Qr004236 9, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 08:28:05 -0600 (CST) 9, 35 -- Received: from mxv2.d.umn.edu (mxv2.d.umn.edu [131.212.109.136]) 9, 35 -- by mxr1.d.umn.edu (8.13.8/8.13.8) with ESMTP id q13ES5GH004432 9, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 08:28:05 -0600 (CST) 9, 35 -- Received: from mx4-g.d.umn.edu (mx4-g.d.umn.edu [131.212.109.174]) 9, 35 -- by mxv2.d.umn.edu (8.13.8/8.13.8) with ESMTP id q13ES08S022070 9, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 08:28:03 -0600 (CST) 9, 35 -- Received: from mail-iy0-f176.google.com (mail-iy0-f176.google.com [209.85.210.176]) 9, 35 -- by mx4-g.d.umn.edu (8.13.8/8.13.8) with ESMTP id q13EQXRs028768 9, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 08:27:08 -0600 (CST) 9, 35 -- Received: by mail-iy0-f176.google.com with SMTP id z35so6720094iag.21 9, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 03 Feb 2012 06:27:08 -0800 (PST) 9, 35 -- MIME-Version: 1.0 9, 35 -- Received: by 10.50.202.97 with SMTP id kh1mr8731762igc.19.1328279227538; Fri, 9, 35 -- 03 Feb 2012 06:27:07 -0800 (PST) 9, 35 -- Received: by 10.231.105.201 with HTTP; Fri, 3 Feb 2012 06:27:07 -0800 (PST) 9, 35 -- In-Reply-To: {201202021953.q12JrgsD028932-at-ns.microscopy.com} 9, 35 -- References: {201202021953.q12JrgsD028932-at-ns.microscopy.com} 9, 35 -- Date: Fri, 3 Feb 2012 08:27:07 -0600 9, 35 -- Message-ID: {CAP4dfZUN_4DHeDCTDb-sJv+-hwTqLZJC+ye5gRM9+7FY0smcLA-at-mail.gmail.com} 9, 35 -- Subject: Re: [Microscopy] viaWWW:SDD - standby or operate mode 9, 35 -- From: Bryan Bandli {bbandli-at-d.umn.edu} 9, 35 -- To: Microscopy-at-microscopy.com 9, 35 -- Cc: pscallio-at-dal.ca 9, 35 -- Content-Type: text/plain; charset=ISO-8859-1 9, 35 -- X-Virus-Scanned: clamav-milter 0.96.5 at mxv2.d.umn.edu 9, 35 -- X-Virus-Status: Clean 9, 35 -- Content-Transfer-Encoding: 8bit 9, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q13EUb7d021792 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mary.walker-at-csl.com.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mary.walker-at-csl.com.au Name: mary walker
Message: Do any listers have experience with counting virus particles with latex beads? Is it possible to do better than order of magnitude? What is the minimum number of particles per ml that can be counted? What is the lower limit?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dvsridhararao-at-yahoo.co.in as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] TEM specimen preparation for semiconductors
Message: Sir/Madam,
We are in the process of procuring a polishing equipment for preparing plan-view and cross-sectional TEM specimens of semiconductor thin films, especially III-arsenides and III-Nitrides. In this context, we have come across the Buehler's product MPC 2000 (weblink given below):
May I request the users of this equipment/those familiar with this equipment, to kindly give their valuable feed back on the functioning/suitability of this equipment for TEM specimen preparation? Login Host: 59.93.72.197 ---------------------------------------------------------------------------
==============================Original Headers============================== 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 3 09:36:28 2012 10, 25 -- Received: from znl.com ([206.69.208.20]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13FaSJV016864 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 09:36:28 -0600 10, 25 -- Received: from localhost (localhost [127.0.0.1]) 10, 25 -- by znl.com (Postfix) with ESMTP id DE665485151 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 09:36:28 -0600 (CST) 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 25 -- Received: from znl.com ([127.0.0.1]) 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 25 -- with ESMTP id FKVZNn9E4Ccl for {microscopy-at-microscopy.com} ; 10, 25 -- Fri, 3 Feb 2012 09:36:28 -0600 (CST) 10, 25 -- Received: from NestorPwrBk.local (dsl-58-6-4-117.wa.westnet.com.au [58.6.4.117]) 10, 25 -- by znl.com (Postfix) with ESMTPA id 66802485146 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 09:36:27 -0600 (CST) 10, 25 -- Message-ID: {4F2BFEF7.6010504-at-microscopy.com} 10, 25 -- Date: Fri, 03 Feb 2012 23:36:23 +0800 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 25 -- Reply-To: dvsridhararao-at-yahoo.co.in 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 25 -- MIME-Version: 1.0 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 25 -- Subject: viaWWW:TEM specimen preparation for semiconductors 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dieter.bosshardt-at-zmk.unibe.ch as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: School of Dental Medicine, University of Bern, Switzerland
Title-Subject: [Filtered] immunohistochemistry with anti-VEGF antibody
Message: Hi I was wondering if anyone has any experience of immunohistochemical staining for VEGF in the human dental pulp? I am currently trying to stain the vasculature, but am experiencing a lot of background collagen staining too, making differentiation impossible! I am using Polyclonal Goat IgG VEGF antibody, species reactivity human (R&D systems), together with their HRP-AEC kit. On the positive and negative control tissues (placenta) it has worked really well, with the blood vessels outlined clearly by the stain:
My tissue of interest is, however the human pulp, I am using 5μm sections of teeth which have been fixed in formalin for 48hrs, decalcified in 10% formic acid and then processed and paraffin embedded. Despite the VEGF protocol working really well on the control tissues the background staining (mainly collagen) is really high in my tissue sections at both the recommended working dilution (1:20, and a much lower dilution 1:100):
Does anyone have any experience of staining for VEGF in such tissue sections, and/or do they have any practical advice about how to eliminate/reduce dramatically this background staining? Kind regards Dieter Bosshardt
We also have an Oxford SDD and the technologist who installed it recommended that we keep it in standby mode when not being used in the near future.
Translation: if it would be used within 12-24 hr, keep it chilled. Any longer periods, go to standby.
My personal opinion is keeping it chilled all the time is putting an unnecessary "strain" on the Peltier electronics.
-- John J. Bozzola, Ph.D. Professor & Blissfully Retired Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University Carbondale, ILÂ 62901
==============================Original Headers============================== 6, 20 -- From bozzola-at-siu.edu Fri Feb 3 11:49:19 2012 6, 20 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13HnJBP004063 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 11:49:19 -0600 6, 20 -- Received: by vbip1 with SMTP id p1so3308219vbi.0 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 03 Feb 2012 09:49:19 -0800 (PST) 6, 20 -- MIME-Version: 1.0 6, 20 -- Received: by 10.52.72.107 with SMTP id c11mr3996306vdv.48.1328291359255; Fri, 6, 20 -- 03 Feb 2012 09:49:19 -0800 (PST) 6, 20 -- Received: by 10.52.188.200 with HTTP; Fri, 3 Feb 2012 09:49:19 -0800 (PST) 6, 20 -- In-Reply-To: {201202031431.q13EVB1Q023653-at-ns.microscopy.com} 6, 20 -- References: {201202031431.q13EVB1Q023653-at-ns.microscopy.com} 6, 20 -- Date: Fri, 3 Feb 2012 11:49:19 -0600 6, 20 -- Message-ID: {CAKeCJ9yu_mLHAJf=Dip0QxRJn5KnAYFPRt_QXdxDY+ScxCipWw-at-mail.gmail.com} 6, 20 -- Subject: Re: [Microscopy] Re: viaWWW:SDD - standby or operate mode 6, 20 -- From: John Bozzola {bozzola-at-siu.edu} 6, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 6, 20 -- Content-Type: text/plain; charset=UTF-8 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q13HnJBP004063 ==============================End of - Headers==============================
I have spoken with Oxford's applications people and they suggest leaving it in Standby except when needed. It takes no more than 5 minutes to cool down to the operating temperature.
Alan
At 08:31 AM 2/3/2012, bbandli-at-d.umn.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Alan W Nicholls, PhD Associate Director, RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
In recent years we have been getting requests to image functionalized nanoparticles, such as 10 nm gold with proteins or peptides attached. Generally the clients want to know how thick or large the organic "shell" is, or how the protein/peptide is arranged on the surface. Most of our experience has been with either inorganic particles (which we view without staining) or larger organic structures such as viral procapsids or large proteins (e.g., myosin) or protein assemblies, which we negative stain. My questions for this list are as follows:
- have others on the list had good luck imaging these kinds of conjugates at the EM level, and if so, using what techniques? - how much organic material must one have associated with the nanoparticle to see it by negative staining or other methods? For example, how hard is it to detect a 150 kD protein on a 10 nm gold particle? An example would be IgG attached to a 10 nm gold particle. - can you suggest any publications or review articles on the subject? For some reason I'm not having much luck with my Pubmed search strategies. A recent paper by Cao and Mao seems to deal mainly with small gold particles on much larger protein aggregates, but perhaps there are others out there.
Many thanks (in advance) for your suggestions.
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 5, 18 -- From marie.cantino-at-uconn.edu Fri Feb 3 13:07:15 2012 5, 18 -- Received: from mta1.uits.uconn.edu (mailscanner.uconn.edu [137.99.25.234]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13J7FK2006567 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 13:07:15 -0600 5, 18 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9]) 5, 18 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 4D02634B9A 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 14:07:15 -0500 (EST) 5, 18 -- Message-Id: {6803418B-8131-4A98-990F-158505836E6E-at-uconn.edu} 5, 18 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 5, 18 -- To: Microscopy-at-Microscopy.Com 5, 18 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 18 -- Content-Transfer-Encoding: 7bit 5, 18 -- Mime-Version: 1.0 (Apple Message framework v936) 5, 18 -- Subject: TEM of functionalized nanoparticles 5, 18 -- Date: Fri, 3 Feb 2012 14:07:15 -0500 5, 18 -- X-Mailer: Apple Mail (2.936) 5, 18 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 5, 18 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Would like to explore the experiences and words of wisdom on the idea of having a "core analytical facility " on a University campus. Questions I am exploring are: How is this idea presented to administrators as a needed addition to support research activities across many disciplines? Or is it? How are such facilities managed and what are reasonable staffing levels. What drives the tool set? Is it internal existing research or potential outside research partnerships? Finally most important is addressing how to pay for and continually support such a facility?
Thanks for any thoughts or ideas on the subject.
Roy Beavers Southern Methodist University Department of Earth Sciences 3225 Daniel Ave Dallas, TX 75205 Voice: 214-768-2756 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 6, 27 -- From rbeavers-at-mail.smu.edu Fri Feb 3 13:31:32 2012 6, 27 -- Received: from smtap1.systems.smu.edu (smtap1.systems.smu.edu [129.119.65.148]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13JVWjS023869 6, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 13:31:32 -0600 6, 27 -- X-IronPort-Anti-Spam-Filtered: true 6, 27 -- X-IronPort-Anti-Spam-Result: Av0EAOE1LE+Bd0GG/2dsb2JhbABAA68ngQWBdAVxGgEqViYBBBMIoEWXEokFi1YEAQUCAhYGAgYBBwINHw8DgyBtGR8Ygj5jBIgRn28 6, 27 -- Received: from sxht1p3.systems.smu.edu ([129.119.65.134]) 6, 27 -- by smtah1.systems.smu.edu with ESMTP/TLS/AES128-SHA; 03 Feb 2012 13:31:26 -0600 6, 27 -- Received: from SXMB1PG.SYSTEMS.SMU.EDU ([fe80::54f4:609f:2958:17b2]) by 6, 27 -- SXHT1P3.SYSTEMS.SMU.EDU ([2002:8177:4186::8177:4186]) with mapi id 6, 27 -- 14.01.0355.002; Fri, 3 Feb 2012 13:31:25 -0600 6, 27 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 6, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 27 -- Subject: Process of building "Academic Core Facilities" 6, 27 -- Thread-Topic: Process of building "Academic Core Facilities" 6, 27 -- Thread-Index: AcziqFWURTjEs0p3Q9aLU5WNhenJUQ== 6, 27 -- Date: Fri, 3 Feb 2012 19:31:25 +0000 6, 27 -- Message-ID: {BC0C36E74D341741A25CE2ABB122E7A32A374E62-at-SXMB1PG.SYSTEMS.SMU.EDU} 6, 27 -- Accept-Language: en-US 6, 27 -- Content-Language: en-US 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- x-originating-ip: [129.119.70.150] 6, 27 -- Content-Type: text/plain; charset="iso-8859-1" 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q13JVWjS023869 ==============================End of - Headers==============================
I have done a lot of work with Fungal samples whose walls are mostly chitin. The walls routinely stained well with OsO4, Uac, and PbCit - sorry I never did a test to see which predominantly stained the chitin. However, I have used Barium Permanganate specifically for stainging fungal walls for TEM and it does a great job. Original reference is:
Hoch, H. C. 1977. Use of permanganate of increase the electron opacity of fungal walls. Mycologia 69:1209-2113.
On 31 Jan 2012 at 15:34, lgordon-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Dear Listserv, } } I have a chitin hydrogel that I would like to image in TEM. I did some } research and there are reports that standard EM heavy metal stains } (osmium, UA, lead citrate) will stain chitin, however, there are also } papers that indicate that chitin doesn't pick up these stains. I was } wondering if anyone has any experiance staining chitin for TEM. } } Ideally, a protocol to stain sections on a grid would be best as I } have some un-stained sections (there is iron oxide in the gel which I } wanted to visualize without any stain). If this isn't possible then I } can process some additional samples. } } Thanks very much, } Lyle } } -- } Lyle Gordon } Department of Materials Science and Engineering } Northwestern University } } 2220 Campus Drive } Evanston, IL 60208 } } Tel: (847) 491-3584 } lgordon-at-u.northwestern.edu } } ==============================Original } Headers============================== 7, 23 -- From lgordon-at-gmail.com } Tue Jan 31 14:33:38 2012 7, 23 -- Received: from } mail-lpp01m010-f41.google.com (mail-lpp01m010-f41.google.com } [209.85.215.41]) 7, 23 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id q0VKXbnj013643 7, 23 -- for } {microscopy-at-microscopy.com} ; Tue, 31 Jan 2012 14:33:38 -0600 7, 23 -- } Received: by lamf4 with SMTP id f4so310098lam.0 7, 23 -- for } {microscopy-at-microscopy.com} ; Tue, 31 Jan 2012 12:33:37 -0800 (PST) 7, } 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 23 -- } d=gmail.com; s=gamma; 7, 23 -- } h=mime-version:from:date:message-id:subject:to:content-type; 7, 23 -- } bh=fAVfgQt8embJHdxesgcDvXPEH/Syq3PdaSq3ZVI3Tog=; 7, 23 -- } b=DHSEEyKH90yWDvwCxlJNp3g768nN8qYLe1YuN2MIC5nD+z2LrZ5vOzdcZsBFcfKUEu } 7, 23 -- } OR5gn2dsEDbfEmflZADE43TZyXqwc+tE3mwwZjl0Z5P/V5i/TPnPAwG957H/HKLcBFEN } 7, 23 -- Gu8IHEJN1/W7A4vMnBBufzU7zlZuVw9IfQjTE= 7, 23 -- } Received: by 10.112.103.168 with SMTP id } fx8mr3454295lbb.14.1328042017233; 7, 23 -- Tue, 31 Jan 2012 12:33:37 } -0800 (PST) 7, 23 -- MIME-Version: 1.0 7, 23 -- Received: by } 10.112.27.37 with HTTP; Tue, 31 Jan 2012 12:33:17 -0800 (PST) 7, 23 -- } From: Lyle Gordon {lgordon-at-gmail.com} 7, 23 -- Date: Tue, 31 Jan 2012 } 14:33:17 -0600 7, 23 -- Message-ID: } {CAFhTNAmT=KfvvLPP42OZkZTXCe07vLuqsz5PkkP_TsrgRZvOFA-at-mail.gmail.com} } 7, 23 -- Subject: Staining Chitin for TEM 7, 23 -- To: } microscopy-at-microscopy.com 7, 23 -- Content-Type: text/plain; } charset=ISO-8859-1 ==============================End of - } Headers==============================
Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.cami.muohio.edu
==============================Original Headers============================== 9, 24 -- From edelmare-at-muohio.edu Fri Feb 3 13:51:34 2012 9, 24 -- Received: from mualmarp01.mcs.muohio.edu (mualmarp01.mcs.muohio.edu [134.53.6.73]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13JpYXj008594 9, 24 -- for {microscopy-at-Microscopy.com} ; Fri, 3 Feb 2012 13:51:34 -0600 9, 24 -- Received: from mualmafp01.mcs.muohio.edu (mualmafp01.mcs.muohio.edu [134.53.6.9]) 9, 24 -- by mualmarp01.mcs.muohio.edu (8.13.8/8.13.8) with ESMTP id q13JpXxx016140; 9, 24 -- Fri, 3 Feb 2012 14:51:33 -0500 9, 24 -- Received: from [10.34.160.232] ([10.34.160.232]) 9, 24 -- by mualmafp01.mcs.muohio.edu (8.13.8/8.13.8) with ESMTP id q13JpXQu019411; 9, 24 -- Fri, 3 Feb 2012 14:51:33 -0500 9, 24 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 9, 24 -- To: "lgordon-at-gmail.com" {lgordon-at-gmail.com} 9, 24 -- Date: Fri, 03 Feb 2012 14:51:32 -0500 9, 24 -- MIME-Version: 1.0 9, 24 -- Subject: Re: [Microscopy] Staining Chitin for TEM 9, 24 -- CC: microscopy-at-Microscopy.com 9, 24 -- Message-ID: {4F2BF474.29719.57589FF2-at-edelmare.muohio.edu} 9, 24 -- Priority: normal 9, 24 -- In-reply-to: {201201312034.q0VKYVnZ014480-at-ns.microscopy.com} 9, 24 -- References: {201201312034.q0VKYVnZ014480-at-ns.microscopy.com} 9, 24 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 24 -- Content-type: text/plain; charset=US-ASCII 9, 24 -- Content-transfer-encoding: 7BIT 9, 24 -- Content-description: Mail message body ==============================End of - Headers==============================
On behalf of the Microanalysis Society (MAS), I invite you to the 3rd Topical Conference on EBSD, which will be held at Carnegie Mellon University on June 19-21, 2012. We have fantastic new speakers, and we are once again providing a comprehensive tutorial on EBSD that will include live demonstrations. For more information, and to register, please visit our website:
Space is limited, so please register early. We look forward to seeing you in Pittsburgh!
Best regards, Andrew Deal
MAS Director, EBSD 2012 Organizing Committee Chair
Thanks, Andy ******************************************** Andrew Deal, PhD Materials Scientist GE Global Research One Research Circle Building K1, Room 1D-37A Niskayuna, NY 12309 US T +1 518 387 5456 F +1 518 387 6905 ********************************************
==============================Original Headers============================== 3, 19 -- From jfmjfm-at-umich.edu Fri Feb 3 15:42:22 2012 3, 19 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13LgMWZ005162 3, 19 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 15:42:22 -0600 3, 19 -- Received: FROM [192.168.1.81] (dsl-58-6-4-117.wa.westnet.com.au [58.6.4.117]) 3, 19 -- By hackers.mr.itd.umich.edu ID 4F2C54BA.3362E.19414 ; 3, 19 -- Authuser jfmjfm; 3, 19 -- 3 Feb 2012 16:42:19 EST 3, 19 -- Content-Type: text/plain; charset=us-ascii 3, 19 -- Subject: 3rd Topical Conference on EBSD 3, 19 -- Mime-Version: 1.0 (Apple Message framework v1257) 3, 19 -- From: John Mansfield {jfmjfm-at-umich.edu} 3, 19 -- Date: Fri, 3 Feb 2012 16:42:13 -0500 3, 19 -- Reply-To: "Deal, Andrew D (GE Global Research)" {deal-at-research.ge.com} 3, 19 -- Message-Id: {0EE0A305-A075-4BF7-9002-12A1652AEB3B-at-umich.edu} 3, 19 -- To: microscopy-at-microscopy.com 3, 19 -- X-Mailer: Apple Mail (2.1257) 3, 19 -- Content-Transfer-Encoding: 8bit 3, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q13LgMWZ005162 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both deal-at-research.ge.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
On behalf of the Microanalysis Society (MAS), I invite you to the 3rd Topical Conference on EBSD, which will be held at Carnegie Mellon University on June 19-21, 2012. We have fantastic new speakers, and we are once again providing a comprehensive tutorial on EBSD that will include live demonstrations. For more information, and to register, please visit our website: http://www.microbeamanalysis.org/topical-conferences/ebsd-2012/
Space is limited, so please register early. We look forward to seeing you in Pittsburgh!
Best regards, Andrew Deal
MAS Director, EBSD 2012 Organizing Committee Chair
For any of you out there who think they might like to try administration, a position has opened up at Purdue and we (other interested microscopists on campus) are hoping for someone who is a strong advocate of microscopy.
Take a look and pass this on if you know of someone who might fit the bill.
++++++++++++++++++++++++++++++++++++++++++++++
Head and Professor School of Materials Engineering
Purdue University is seeking nominations and applications for the position of Head of the School of Materials Engineering. A dynamic leader is sought to advance the School¹s nationally-ranked program and to enhance its international and national impact through a continuing commitment to excellence in discovery, learning and engagement. The Head provides vision and leadership to the faculty, students, staff, alumni, and other stakeholders of the School, and will have the opportunity to shape and implement the strategic plan for the School and the College of Engineering.
The Head must have a Ph.D. in Materials Science and Engineering or related disciplines, qualify for an appointment at the full professor level with tenure, have a distinguished academic, government or industrial record, and demonstrate strong leadership and collaborative skills. Candidates should have a clear understanding of the current needs and future direction of the materials science and engineering profession, possess a commitment to diversity and collaboration, and be skilled in administration, student relations, mentoring, and alumni development.
The faculty members in the School of Materials Engineering are located in the Neil Armstrong Hall of Engineering and the Birck Nanotechnology Center, which provide outstanding facilities for teaching and research. The School currently has 116 undergraduate students, 89 graduate students and postdocs, and 20 faculty members. The School¹s faculty members have strong connections to research centers in Purdue¹s Discovery Park, as well as with the Colleges of Agriculture, Liberal Arts, Pharmacy, Management, and Science.
The College of Engineering at Purdue consists of over 7300 undergraduate students, nearly 2,900 graduate students, and 359 faculty members. Purdue is one of the nation¹s leading land-grant universities with a full range of academic majors and an enrollment of over 40,000 students on the West Lafayette campus.
An application should include: (1) a three-page personal statement addressing the applicant's vision, administrative philosophy, experience and qualifications; (2) a curriculum vitae; and (3) names and contact information for at least three references. Applications and inquiries will be kept confidential. Applicants will be notified before references are contacted. Electronic submission is preferred and should be uploaded at https://engineering.purdue.edu/Engr/InfoFor/Employment. Screening will commence February 1, 2012, and continue until this position is filled. Nominations and questions regarding the position can be addressed to Chair, Materials Engineering Head Search Committee, Purdue University, Neil Armstrong Hall of Engineering, 701 W. Stadium Avenue, West Lafayette, IN 47907-2045, Phone: 765-494-5012, Email: mse-search-at-ecn.purdue.edu. A background check will be required for employment in this position. Purdue University is an equal opportunity/equal access/affirmative action employer fully committed to achieving a diverse workforce.
Debby
-- Debby Sherman, Interim Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 13, 28 -- From dsherman-at-purdue.edu Sat Feb 4 09:49:39 2012 13, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q14FndM9025492 13, 28 -- for {microscopy-at-microscopy.com} ; Sat, 4 Feb 2012 09:49:39 -0600 13, 28 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 13, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id q14FndnK013157 13, 28 -- for {microscopy-at-microscopy.com} ; Sat, 4 Feb 2012 10:49:39 -0500 13, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.60]) by WPPEXHUB04H.purdue.lcl 13, 28 -- ([::1]) with mapi; Sat, 4 Feb 2012 10:49:38 -0500 13, 28 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 13, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 13, 28 -- Date: Sat, 4 Feb 2012 10:49:36 -0500 13, 28 -- Subject: Materials Science Department Head position 13, 28 -- Thread-Topic: Materials Science Department Head position 13, 28 -- Thread-Index: AczjVJheJQv3nJ9uNUGhi7n40aLUxQ== 13, 28 -- Message-ID: {CB52BDC0.16D75%dsherman-at-purdue.edu} 13, 28 -- Accept-Language: en-US 13, 28 -- Content-Language: en-US 13, 28 -- X-MS-Has-Attach: 13, 28 -- X-MS-TNEF-Correlator: 13, 28 -- user-agent: Microsoft-Entourage/13.12.0.111121 13, 28 -- acceptlanguage: en-US 13, 28 -- Content-Type: text/plain; charset="iso-8859-1" 13, 28 -- MIME-Version: 1.0 13, 28 -- X-PMX-Version: 5.5.9.388399 13, 28 -- X-PerlMx-Virus-Scanned: Yes 13, 28 -- Content-Transfer-Encoding: 8bit 13, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q14FndM9025492 ==============================End of - Headers==============================
Hi all, We have an otherwise perfectly fine Sorvall RC2-B which failed it's recent safety check because the inner plastic cover of the lid has a crack. Of course, no parts can be found for that centrifuge anymore. Thus, if you are trashing your old RC2-B, it would be great if we could get the plastic cover which is just screwed to the lid from the inside.
Thanks in advance for any help,
Anja Seybert Research Scientist Frankfurt am Main, Germany
==============================Original Headers============================== 4, 19 -- From seybert-at-biophysik.org Mon Feb 6 02:47:20 2012 4, 19 -- Received: from atlas.biophys.uni-frankfurt.de (atlas.biophys.uni-frankfurt.de [141.2.213.147]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q168lJpr032346 4, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 02:47:20 -0600 4, 19 -- Received: (qmail 26455 invoked from network); 6 Feb 2012 09:47:19 +0100 4, 19 -- Received: from unknown (HELO www.biophysik-ssl.de) (127.0.0.1) 4, 19 -- by 127.0.0.1 with SMTP; 6 Feb 2012 09:47:19 +0100 4, 19 -- Date: Mon, 6 Feb 2012 09:46:57 +0100 4, 19 -- To: Microscopy-at-microscopy.com 4, 19 -- From: Anja Seybert {seybert-at-biophysik.org} 4, 19 -- Subject: Looking for: Inner lid of Sorvall RC2-B 4, 19 -- Message-ID: {39835fe366c5c89feb7aac2201da472d-at-www.biophysik-ssl.de} 4, 19 -- X-Priority: 3 4, 19 -- X-Mailer: PHPMailer 5.1 (phpmailer.sourceforge.net) 4, 19 -- X-Mailer: FeLaMiMail 4, 19 -- MIME-Version: 1.0 4, 19 -- Content-Type: text/plain; charset="utf-8" 4, 19 -- Content-Transfer-Encoding: 8bit 4, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q168lJpr032346 ==============================End of - Headers==============================
I am afraid I am not a specialist of this technique but I know how it works and I would like to just make some general comments. Others with more experience may want to give a more precise answer.
This method involves statistical analysis, which means the more you sample, the more precise your number. In other words, the precision of your analysis will basically depend on how much time you want to put into your counting (how many grids/fields you actually count). Based on that, I assume that you may be able to count fewer numbers (decrease your lower limit) by counting many grids.
Best regards, Stephane
________________________________ X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: nizets2-at-yahoo.com Sent: Friday, February 3, 2012 4:40 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mary.walker-at-csl.com.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mary.walker-at-csl.com.au Name: mary walker
Message: Do any listers have experience with counting virus particles with latex beads? Is it possible to do better than order of magnitude? What is the minimum number of particles per ml that can be counted? What is the lower limit?
I like these projects which put the techniques to their limits. I think the reason why you have difficulties finding publications on this matter and that the question is not trivial at all. First of all, and to start with a relaxed note, the mixing of kDa und nm in one sentence made me smile because it makes as much sense as asking if 1kg of something fits in one meter. The Dalton is a unit of mass and does not give a good indication of the dimension or the shape of a molecule. That said, I seem to remember from the past answers of our emminent immunoglobulinologist Jan Leunissen that the size of an IgG is several nm, I think more than 5nm. Which means, in your case, that you should not expect more that a few molecules per nanoparticle, except if they form multilayers. As for the techniques, I wonder if a SEM could resolve this structure. Although I have experience with analytical low res SEMs, I don't know the limits of modern FEG SEMs. I would be happy if some listers would care to share their opinion on this matter. It seems that AFM (atomic force microscopy) would be able to resolve this structure, but I wonder if it would be possible in this condition because AFM works on flat substrate and here you have round particles. It would probably be possible to find some answers using cry-microscopy, the same way scientists use it to resolve viral structures at several anstrom resolution. It probably depends on how much time and money your clients are wanting to spend and how critical this information is for them.
Regards, Stephane
----- Original Message ----- X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} To: nizets2-at-yahoo.com Cc: Sent: Friday, February 3, 2012 8:10 PM
In recent years we have been getting requests to image functionalized nanoparticles, such as 10 nm gold with proteins or peptides attached. Generally the clients want to know how thick or large the organic "shell" is, or how the protein/peptide is arranged on the surface. Most of our experience has been with either inorganic particles (which we view without staining) or larger organic structures such as viral procapsids or large proteins (e.g., myosin) or protein assemblies, which we negative stain. My questions for this list are as follows:
- have others on the list had good luck imaging these kinds of conjugates at the EM level, and if so, using what techniques? - how much organic material must one have associated with the nanoparticle to see it by negative staining or other methods? For example, how hard is it to detect a 150 kD protein on a 10 nm gold particle? An example would be IgG attached to a 10 nm gold particle. - can you suggest any publications or review articles on the subject? For some reason I'm not having much luck with my Pubmed search strategies. A recent paper by Cao and Mao seems to deal mainly with small gold particles on much larger protein aggregates, but perhaps there are others out there.
Many thanks (in advance) for your suggestions.
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 5, 18 -- From marie.cantino-at-uconn.edu Fri Feb 3 13:07:15 2012 5, 18 -- Received: from mta1.uits.uconn.edu (mailscanner.uconn.edu [137.99.25.234]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13J7FK2006567 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 13:07:15 -0600 5, 18 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9]) 5, 18 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 4D02634B9A 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 14:07:15 -0500 (EST) 5, 18 -- Message-Id: {6803418B-8131-4A98-990F-158505836E6E-at-uconn.edu} 5, 18 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 5, 18 -- To: Microscopy-at-Microscopy.Com 5, 18 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 18 -- Content-Transfer-Encoding: 7bit 5, 18 -- Mime-Version: 1.0 (Apple Message framework v936) 5, 18 -- Subject: TEM of functionalized nanoparticles 5, 18 -- Date: Fri, 3 Feb 2012 14:07:15 -0500 5, 18 -- X-Mailer: Apple Mail (2.936) 5, 18 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 5, 18 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 15, 42 -- From nizets2-at-yahoo.com Mon Feb 6 04:28:31 2012 15, 42 -- Received: from nm9.bullet.mail.sp2.yahoo.com (nm9.bullet.mail.sp2.yahoo.com [98.139.91.79]) 15, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q16ASUSx003271 15, 42 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 04:28:31 -0600 15, 42 -- Received: from [98.139.91.62] by nm9.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 15, 42 -- Received: from [98.139.91.23] by tm2.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 15, 42 -- Received: from [127.0.0.1] by omp1023.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 15, 42 -- X-Yahoo-Newman-Property: ymail-3 15, 42 -- X-Yahoo-Newman-Id: 481221.57336.bm-at-omp1023.mail.sp2.yahoo.com 15, 42 -- Received: (qmail 64306 invoked by uid 60001); 6 Feb 2012 10:28:30 -0000 15, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1328524109; bh=+xZoCP0S7BlCXixrJBv6fCBgmv9JLm9A9772ftf9y9w=; h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=DhiSJ4sQkcV0vFmz7NomZjwgCjpBj4FAdn7ZD2wksosyYhu8N5W2UqbTQyeXCJK4oVawc69mSzTPOiPLJMDjlVLrxuWj+2HE7+negf7O2gV72PfH1xIgxl0oKW5yjsdKajsyvKAsKy1AyxjksZ4FKsRyicVW6P+XS3bODk3UHBI= 15, 42 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 15, 42 -- s=s1024; d=yahoo.com; 15, 42 -- h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 15, 42 -- b=0z5d16psGKpudCX/OEBR6HSJZodETUvunNzVkE2Bdt/50FwYt985UVUxt+wGQv0dJpC6PSS5C1UVuucgfGoIke8emqTSu5ngf9h5IlUbvg19SDLYgnCq56YYozOelWUhS7ZeiXK6XJoArVPn97DAVKnn36IdAtBG77TEHRcq0XE=; 15, 42 -- X-YMail-OSG: mU9.CMYVM1nU5RWQzCOCo2tCXdcc5rBWDHvkrMrE8dmtNg2 15, 42 -- ghcyqmxIav.mWZe55tGfPx8hB73Ku2Go_g2YcxIYVxYu.xIQcjRB8dOscnIl 15, 42 -- ItGCm03ET3Xk9rdFmB5AyEOTu.5q67LQ3BVRuBgGI4Dyb1JBuFnhh6LX33Ge 15, 42 -- eZCSbcxsrxDfym2SvNF9HjWSjNSQhA3pmSisSwcORyEwAK7pVR6xPyjASZja 15, 42 -- 81UDvA.AJMLbz_lavTzjKSa7tsBX1HKarcZ_GAqFWj8E8vgim8xl_2cb9ajC 15, 42 -- J6x_Et5Oxs8J8PMTbE6fQbAsSz1rSnFYUn_0t2yB5pB4NvXbsBmtxHUE9IAq 15, 42 -- cGxGthxOtfOCAVTV5sIWmT0..AZylbKQ7UAE45AJjl9D4Pl9Kheo._ZrOqI7 15, 42 -- 6JOY6X1VgqTVqTbHUdl2hNXyBKit3PZ5.7H4NFTszDoQ1wMaPo7PGZ7MJj6T 15, 42 -- ox4fhW2_ISZ4ykqYrKGhEE3K0h0_x9_whxj_V4afxT8ssY5stADMbOyv75Hf 15, 42 -- IV65YjtPjKc0RCBkjCk.JYib0q649_W.AcC2G7R7T0R_obP_lnUnSx67zPct 15, 42 -- uAa96Geh729v3Ci.cpDJf6V93rfKrN65tq6cZtHoQ5c95q36LP3UHZ.u.7No 15, 42 -- - 15, 42 -- Received: from [80.122.101.100] by web110815.mail.gq1.yahoo.com via HTTP; Mon, 06 Feb 2012 02:28:29 PST 15, 42 -- X-Mailer: YahooMailWebService/0.8.116.338427 15, 42 -- References: {201202031910.q13JASuh012105-at-ns.microscopy.com} 15, 42 -- Message-ID: {1328524109.50274.YahooMailNeo-at-web110815.mail.gq1.yahoo.com} 15, 42 -- Date: Mon, 6 Feb 2012 02:28:29 -0800 (PST) 15, 42 -- From: Stephane Nizet {nizets2-at-yahoo.com} 15, 42 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 15, 42 -- Subject: Re: [Microscopy] TEM of functionalized nanoparticles 15, 42 -- To: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} 15, 42 -- Cc: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 15, 42 -- In-Reply-To: {201202031910.q13JASuh012105-at-ns.microscopy.com} 15, 42 -- MIME-Version: 1.0 15, 42 -- Content-Type: text/plain; charset=iso-8859-1 15, 42 -- Content-Transfer-Encoding: 8bit 15, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q16ASUSx003271 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Movement of the image when changing the objective lens (i.e. FOCUS) is an indication that the beam is going through the objective lens at an angle, i.e. not on the optic axis. The correction step is usually referred to as a "Rotation Calibration" or Objective lens "Current Centering".
It is difficult to describe the exact steps to correct this without knowing which microscope you have since the knobs will vary from microscope to microscope.
Many microscopes have a direct alignment step labelled "rotation center" which oscillates the objective lens. Start with the image close to focus, engage the "rotation center" function (start the objective lens current oscillation) and adjust the correction knobs to minimize the image movement.
For a non-computerized microscope, there may be a set of knobs labelled "rotation center". In this case, focus the image and set a recognizable feature in the center. Then defocus and watch the feature shift away from the center. Use the "rotation center" knobs to recenter the feature while the image is defocused. Repeat if necessary.
Good luck, Henk
At 2/6/2012 9:11 AM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both ravi.thakkar369-at-gmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com Name: Ravindra Thakkar } } Title-Subject: [Filtered] TEM : Focusing Problem. } } Message: Image is not getting focused. The image is drastically moving } while focusing, it moving highly even at low focus step& at low } magnification. } } So how to resolve this problem. } } Login Host: 14.139.97.76 } --------------------------------------------------------------------------- }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 9, 26 -- From colijn.1-at-osu.edu Mon Feb 6 08:37:46 2012 9, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16Ebjv8009923 9, 26 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 08:37:46 -0600 9, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 9, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 26 -- id {01OBNYUKL30093JRTP-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 9, 26 -- Mon, 06 Feb 2012 09:37:45 -0500 (EST) 9, 26 -- Received: from [164.107.76.202] (hoc3.ceof.ohio-state.edu [164.107.76.202]) 9, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 26 -- with ESMTPA id {01OBNYUJTXD093JGJW-at-ecr6.ohio-state.edu} ; Mon, 9, 26 -- 06 Feb 2012 09:37:44 -0500 (EST) 9, 26 -- Date: Mon, 06 Feb 2012 09:37:43 -0500 9, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 26 -- Subject: Re: [Microscopy] viaWWW:TEM : Focusing Problem. 9, 26 -- In-reply-to: {201202061411.q16EBSfv026644-at-ns.microscopy.com} 9, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 26 -- To: microscopy-at-microscopy.com, ravi.thakkar369-at-gmail.com 9, 26 -- Message-id: {4F2FE5B7.3010903-at-osu.edu} 9, 26 -- MIME-version: 1.0 9, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 26 -- Content-transfer-encoding: 7bit 9, 26 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:9.0) Gecko/20111222 9, 26 -- Thunderbird/9.0.1 9, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 26 -- References: {201202061411.q16EBSfv026644-at-ns.microscopy.com} ==============================End of - Headers==============================
I do understand the difference between mass and length.
The problem is that the clients usually can only give us the molecular weight and (if we're lucky) the number of molecules per particle. If they knew how the proteins/peptides were arranged or the linear dimensions of the coating they wouldn't need to come to me. What I'm trying to find out from the listserver or the literature is whether we can reasonably expect to be able to detect a medium sized protein such as IgG on an electron dense gold particle, given the various artifacts of techniques such as negative staining.
We only have TEM (w/a tungsten emitter) and FESEM in our facility, but I can send them elsewhere for AFM.
Marie
On Feb 6, 2012, at 5:33 AM, nizets2-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Marie, } } I like these projects which put the techniques to their limits. } I think the reason why you have difficulties finding publications on } this matter and that the question is not trivial at all. } First of all, and to start with a relaxed note, the mixing of kDa } und nm in one sentence made me smile because it makes as much sense } as asking if 1kg of something fits in one meter. } The Dalton is a unit of mass and does not give a good indication of } the dimension or the shape of a molecule. } That said, I seem to remember from the past answers of our emminent } immunoglobulinologist Jan Leunissen that the size of an IgG is } several nm, I think more than 5nm. } Which means, in your case, that you should not expect more that a } few molecules per nanoparticle, except if they form multilayers. } As for the techniques, I wonder if a SEM could resolve this } structure. Although I have experience with analytical low res SEMs, } I don't know the limits of modern FEG SEMs. } I would be happy if some listers would care to share their opinion } on this matter. } It seems that AFM (atomic force microscopy) would be able to resolve } this structure, but I wonder if it would be possible in this } condition because AFM works on flat substrate and here you have } round particles. } It would probably be possible to find some answers using cry- } microscopy, the same way scientists use it to resolve viral } structures at several anstrom resolution. It probably depends on how } much time and money your clients are wanting to spend and how } critical this information is for them. } } Regards, } Stephane } } ----- Original Message ----- } X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} } To: nizets2-at-yahoo.com } Cc: } Sent: Friday, February 3, 2012 8:10 PM } Subject: [Microscopy] TEM of functionalized nanoparticles } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } In recent years we have been getting requests to image functionalized } nanoparticles, such as 10 nm gold with proteins or peptides attached. } Generally the clients want to know how thick or large the organic } "shell" is, or how the protein/peptide is arranged on the surface. } Most of our experience has been with either inorganic particles (which } we view without staining) or larger organic structures such as viral } procapsids or large proteins (e.g., myosin) or protein assemblies, } which we negative stain. My questions for this list are as follows: } } - have others on the list had good luck imaging these kinds of } conjugates at the EM level, and if so, using what techniques? } - how much organic material must one have associated with the } nanoparticle to see it by negative staining or other methods? For } example, how hard is it to detect a 150 kD protein on a 10 nm gold } particle? An example would be IgG attached to a 10 nm gold particle. } - can you suggest any publications or review articles on the subject? } For some reason I'm not having much luck with my Pubmed search } strategies. A recent paper by Cao and Mao seems to deal mainly with } small gold particles on much larger protein aggregates, but perhaps } there are others out there. } } Many thanks (in advance) for your suggestions. } } Dr. Marie E. Cantino } Associate Professor of Physiology and Neurobiology } Director, Electron Microscopy Laboratory } University of Connecticut, Unit 3242 } Phone: 860-486-3588 } Fax: 860-486-6369 } } } ==============================Original } Headers============================== } 5, 18 -- From marie.cantino-at-uconn.edu Fri Feb 3 13:07:15 2012 } 5, 18 -- Received: from mta1.uits.uconn.edu (mailscanner.uconn.edu } [137.99.25.234]) } 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id q13J7FK2006567 } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 } 13:07:15 -0600 } 5, 18 -- Received: from d47h9.public.uconn.edu } (d47h9.public.uconn.edu [137.99.47.9]) } 5, 18 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 4D02634B9A } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 } 14:07:15 -0500 (EST) } 5, 18 -- Message-Id: {6803418B-8131-4A98-990F-158505836E6E-at-uconn.edu} } 5, 18 -- From: Marie Cantino {marie.cantino-at-uconn.edu} } 5, 18 -- To: Microscopy-at-Microscopy.Com } 5, 18 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } delsp=yes } 5, 18 -- Content-Transfer-Encoding: 7bit } 5, 18 -- Mime-Version: 1.0 (Apple Message framework v936) } 5, 18 -- Subject: TEM of functionalized nanoparticles } 5, 18 -- Date: Fri, 3 Feb 2012 14:07:15 -0500 } 5, 18 -- X-Mailer: Apple Mail (2.936) } 5, 18 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 } 5, 18 -- X-Virus-Status: Clean } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 15, 42 -- From nizets2-at-yahoo.com Mon Feb 6 04:28:31 2012 } 15, 42 -- Received: from nm9.bullet.mail.sp2.yahoo.com } (nm9.bullet.mail.sp2.yahoo.com [98.139.91.79]) } 15, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id q16ASUSx003271 } 15, 42 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 04:28:31 } -0600 } 15, 42 -- Received: from [98.139.91.62] by } nm9.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 } 15, 42 -- Received: from [98.139.91.23] by } tm2.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 } 15, 42 -- Received: from [127.0.0.1] by omp1023.mail.sp2.yahoo.com } with NNFMP; 06 Feb 2012 10:28:30 -0000 } 15, 42 -- X-Yahoo-Newman-Property: ymail-3 } 15, 42 -- X-Yahoo-Newman-Id: } 481221.57336.bm-at-omp1023.mail.sp2.yahoo.com } 15, 42 -- Received: (qmail 64306 invoked by uid 60001); 6 Feb 2012 } 10:28:30 -0000 } 15, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=yahoo.com; s=s1024; t=1328524109; bh= } +xZoCP0S7BlCXixrJBv6fCBgmv9JLm9A9772ftf9y9w=; h=X-YMail- } OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply- } To:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content- } Transfer-Encoding; } b } = } DhiSJ4sQkcV0vFmz7NomZjwgCjpBj4FAdn7ZD2wksosyYhu8N5W2UqbTQyeXCJK4oVawc69mSzTPOiPLJMDjlVLrxuWj } +2HE7+negf7O2gV72PfH1xIgxl0oKW5yjsdKajsyvKAsKy1AyxjksZ4FKsRyicVW6P } +XS3bODk3UHBI= } 15, 42 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 15, 42 -- s=s1024; d=yahoo.com; } 15, 42 -- h=X-YMail-OSG:Received:X-Mailer:References:Message- } ID:Date:From:Reply-To:Subject:To:Cc:In-Reply-To:MIME-Version:Content- } Type:Content-Transfer-Encoding; } 15, 42 -- b=0z5d16psGKpudCX/OEBR6HSJZodETUvunNzVkE2Bdt/ } 50FwYt985UVUxt } + } wGQv0dJpC6PSS5C1UVuucgfGoIke8emqTSu5ngf9h5IlUbvg19SDLYgnCq56YYozOelWUhS7ZeiXK6XJoArVPn97DAVKnn36IdAtBG77TEHRcq0XE } =; } 15, 42 -- X-YMail-OSG: mU9.CMYVM1nU5RWQzCOCo2tCXdcc5rBWDHvkrMrE8dmtNg2 } 15, 42 -- } ghcyqmxIav.mWZe55tGfPx8hB73Ku2Go_g2YcxIYVxYu.xIQcjRB8dOscnIl } 15, 42 -- ItGCm03ET3Xk9rdFmB5AyEOTu. } 5q67LQ3BVRuBgGI4Dyb1JBuFnhh6LX33Ge } 15, 42 -- } eZCSbcxsrxDfym2SvNF9HjWSjNSQhA3pmSisSwcORyEwAK7pVR6xPyjASZja } 15, 42 -- } 81UDvA.AJMLbz_lavTzjKSa7tsBX1HKarcZ_GAqFWj8E8vgim8xl_2cb9ajC } 15, 42 -- } J6x_Et5Oxs8J8PMTbE6fQbAsSz1rSnFYUn_0t2yB5pB4NvXbsBmtxHUE9IAq } 15, 42 -- } cGxGthxOtfOCAVTV5sIWmT0..AZylbKQ7UAE45AJjl9D4Pl9Kheo._ZrOqI7 } 15, 42 -- } 6JOY6X1VgqTVqTbHUdl2hNXyBKit3PZ5.7H4NFTszDoQ1wMaPo7PGZ7MJj6T } 15, 42 -- } ox4fhW2_ISZ4ykqYrKGhEE3K0h0_x9_whxj_V4afxT8ssY5stADMbOyv75Hf } 15, 42 -- } IV65YjtPjKc0RCBkjCk.JYib0q649_W.AcC2G7R7T0R_obP_lnUnSx67zPct } 15, 42 -- uAa96Geh729v3Ci.cpDJf6V93rfKrN65tq6cZtHoQ5c95q36LP3UHZ.u. } 7No } 15, 42 -- - } 15, 42 -- Received: from [80.122.101.100] by } web110815.mail.gq1.yahoo.com via HTTP; Mon, 06 Feb 2012 02:28:29 PST } 15, 42 -- X-Mailer: YahooMailWebService/0.8.116.338427 } 15, 42 -- References: {201202031910.q13JASuh012105-at-ns.microscopy.com} } 15, 42 -- Message-ID: {1328524109.50274.YahooMailNeo-at-web110815.mail.gq1.yahoo.com } } } 15, 42 -- Date: Mon, 6 Feb 2012 02:28:29 -0800 (PST) } 15, 42 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 15, 42 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } 15, 42 -- Subject: Re: [Microscopy] TEM of functionalized } nanoparticles } 15, 42 -- To: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} } 15, 42 -- Cc: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 15, 42 -- In-Reply-To: {201202031910.q13JASuh012105-at-ns.microscopy.com} } 15, 42 -- MIME-Version: 1.0 } 15, 42 -- Content-Type: text/plain; charset=iso-8859-1 } 15, 42 -- Content-Transfer-Encoding: 8bit } 15, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id q16ASUSx003271 } ==============================End of - } Headers==============================
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 9, 20 -- From marie.cantino-at-uconn.edu Mon Feb 6 08:40:11 2012 9, 20 -- Received: from mta1.uits.uconn.edu (smtp.uconn.edu [137.99.25.234]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16EeB9q015449 9, 20 -- for {microscopy-at-Microscopy.Com} ; Mon, 6 Feb 2012 08:40:11 -0600 9, 20 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9]) 9, 20 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id E24A83499C 9, 20 -- for {microscopy-at-Microscopy.Com} ; Mon, 6 Feb 2012 09:40:10 -0500 (EST) 9, 20 -- Message-Id: {E71EBD9F-CDF7-4B2B-A401-0FFA4339EE6D-at-uconn.edu} 9, 20 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 9, 20 -- To: microscopy-at-Microscopy.Com 9, 20 -- In-Reply-To: {201202061033.q16AXXWw011504-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 9, 20 -- Content-Transfer-Encoding: 7bit 9, 20 -- Mime-Version: 1.0 (Apple Message framework v936) 9, 20 -- Subject: Re: [Microscopy] Re: TEM of functionalized nanoparticles 9, 20 -- Date: Mon, 6 Feb 2012 09:40:10 -0500 9, 20 -- References: {201202061033.q16AXXWw011504-at-ns.microscopy.com} 9, 20 -- X-Mailer: Apple Mail (2.936) 9, 20 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 9, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I am not certain this answers your question but you should be able to detect IgG attached to an electron dense gold particle by negative stain EM. Antibodies are commonly seen attached to virus particles and to colloidal gold particles when negative stain EM is done. Anything you would likely see in your indicated study by AFM can be seen by negative stain EM. All techniques have various artifacts including light-scatter, thin-section EM, AFM, FESEM, and cryo-EM. The key to EM and techniques in general is to learn what is real and what is artifact. It often takes time and work to develop proficiency with many techniques though. Good luck with this , it should be possible.
Charles Humphrey, Ph. D. CDC Atlanta, GA 30333
-----Original Message----- X-from: marie.cantino-at-uconn.edu [mailto:marie.cantino-at-uconn.edu] Sent: Monday, February 06, 2012 9:49 AM To: Humphrey, Charles (CDC/OID/NCEZID)
Hi Stefan,
I do understand the difference between mass and length.
The problem is that the clients usually can only give us the molecular weight and (if we're lucky) the number of molecules per particle. If they knew how the proteins/peptides were arranged or the linear dimensions of the coating they wouldn't need to come to me. What I'm trying to find out from the listserver or the literature is whether we can reasonably expect to be able to detect a medium sized protein such as IgG on an electron dense gold particle, given the various artifacts of techniques such as negative staining.
We only have TEM (w/a tungsten emitter) and FESEM in our facility, but I can send them elsewhere for AFM.
Marie
On Feb 6, 2012, at 5:33 AM, nizets2-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Marie, } } I like these projects which put the techniques to their limits. } I think the reason why you have difficulties finding publications on } this matter and that the question is not trivial at all. } First of all, and to start with a relaxed note, the mixing of kDa und } nm in one sentence made me smile because it makes as much sense as } asking if 1kg of something fits in one meter. } The Dalton is a unit of mass and does not give a good indication of } the dimension or the shape of a molecule. } That said, I seem to remember from the past answers of our emminent } immunoglobulinologist Jan Leunissen that the size of an IgG is several } nm, I think more than 5nm. } Which means, in your case, that you should not expect more that a few } molecules per nanoparticle, except if they form multilayers. } As for the techniques, I wonder if a SEM could resolve this structure. } Although I have experience with analytical low res SEMs, I don't know } the limits of modern FEG SEMs. } I would be happy if some listers would care to share their opinion on } this matter. } It seems that AFM (atomic force microscopy) would be able to resolve } this structure, but I wonder if it would be possible in this condition } because AFM works on flat substrate and here you have round particles. } It would probably be possible to find some answers using cry- } microscopy, the same way scientists use it to resolve viral structures } at several anstrom resolution. It probably depends on how much time } and money your clients are wanting to spend and how critical this } information is for them. } } Regards, } Stephane } } ----- Original Message ----- } X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} } To: nizets2-at-yahoo.com } Cc: } Sent: Friday, February 3, 2012 8:10 PM } Subject: [Microscopy] TEM of functionalized nanoparticles } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } In recent years we have been getting requests to image functionalized } nanoparticles, such as 10 nm gold with proteins or peptides attached. } Generally the clients want to know how thick or large the organic } "shell" is, or how the protein/peptide is arranged on the surface. } Most of our experience has been with either inorganic particles (which } we view without staining) or larger organic structures such as viral } procapsids or large proteins (e.g., myosin) or protein assemblies, } which we negative stain. My questions for this list are as follows: } } - have others on the list had good luck imaging these kinds of } conjugates at the EM level, and if so, using what techniques? } - how much organic material must one have associated with the } nanoparticle to see it by negative staining or other methods? For } example, how hard is it to detect a 150 kD protein on a 10 nm gold } particle? An example would be IgG attached to a 10 nm gold particle. } - can you suggest any publications or review articles on the subject? } For some reason I'm not having much luck with my Pubmed search } strategies. A recent paper by Cao and Mao seems to deal mainly with } small gold particles on much larger protein aggregates, but perhaps } there are others out there. } } Many thanks (in advance) for your suggestions. } } Dr. Marie E. Cantino } Associate Professor of Physiology and Neurobiology Director, Electron } Microscopy Laboratory University of Connecticut, Unit 3242 } Phone: 860-486-3588 } Fax: 860-486-6369 } } } ==============================Original } Headers============================== } 5, 18 -- From marie.cantino-at-uconn.edu Fri Feb 3 13:07:15 2012 5, 18 } -- Received: from mta1.uits.uconn.edu (mailscanner.uconn.edu } [137.99.25.234]) } 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id q13J7FK2006567 } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 } 13:07:15 -0600 } 5, 18 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu } [137.99.47.9]) } 5, 18 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 4D02634B9A } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 } 14:07:15 -0500 (EST) } 5, 18 -- Message-Id: {6803418B-8131-4A98-990F-158505836E6E-at-uconn.edu} } 5, 18 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 5, 18 -- To: } Microscopy-at-Microscopy.Com 5, 18 -- Content-Type: text/plain; } charset=US-ASCII; format=flowed; delsp=yes 5, 18 -- } Content-Transfer-Encoding: 7bit 5, 18 -- Mime-Version: 1.0 (Apple } Message framework v936) 5, 18 -- Subject: TEM of functionalized } nanoparticles 5, 18 -- Date: Fri, 3 Feb 2012 14:07:15 -0500 5, 18 -- } X-Mailer: Apple Mail (2.936) 5, 18 -- X-Virus-Scanned: clamav-milter } 0.96.5 at mta1 5, 18 -- X-Virus-Status: Clean } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 15, 42 -- From nizets2-at-yahoo.com Mon Feb 6 04:28:31 2012 15, 42 -- } Received: from nm9.bullet.mail.sp2.yahoo.com } (nm9.bullet.mail.sp2.yahoo.com [98.139.91.79]) } 15, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id q16ASUSx003271 } 15, 42 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 04:28:31 } -0600 } 15, 42 -- Received: from [98.139.91.62] by } nm9.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 } 15, 42 -- Received: from [98.139.91.23] by } tm2.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 } 15, 42 -- Received: from [127.0.0.1] by omp1023.mail.sp2.yahoo.com } with NNFMP; 06 Feb 2012 10:28:30 -0000 15, 42 -- } X-Yahoo-Newman-Property: ymail-3 15, 42 -- X-Yahoo-Newman-Id: } 481221.57336.bm-at-omp1023.mail.sp2.yahoo.com } 15, 42 -- Received: (qmail 64306 invoked by uid 60001); 6 Feb 2012 } 10:28:30 -0000 } 15, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=yahoo.com; s=s1024; t=1328524109; bh= } +xZoCP0S7BlCXixrJBv6fCBgmv9JLm9A9772ftf9y9w=; h=X-YMail- } OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply- } To:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content- } Transfer-Encoding; } b } = } DhiSJ4sQkcV0vFmz7NomZjwgCjpBj4FAdn7ZD2wksosyYhu8N5W2UqbTQyeXCJK4oVawc6 } 9mSzTPOiPLJMDjlVLrxuWj } +2HE7+negf7O2gV72PfH1xIgxl0oKW5yjsdKajsyvKAsKy1AyxjksZ4FKsRyicVW6P } +XS3bODk3UHBI= } 15, 42 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 15, 42 -- s=s1024; d=yahoo.com; } 15, 42 -- h=X-YMail-OSG:Received:X-Mailer:References:Message- } ID:Date:From:Reply-To:Subject:To:Cc:In-Reply-To:MIME-Version:Content- } Type:Content-Transfer-Encoding; } 15, 42 -- b=0z5d16psGKpudCX/OEBR6HSJZodETUvunNzVkE2Bdt/ } 50FwYt985UVUxt } + } wGQv0dJpC6PSS5C1UVuucgfGoIke8emqTSu5ngf9h5IlUbvg19SDLYgnCq56YYozOelWUh } S7ZeiXK6XJoArVPn97DAVKnn36IdAtBG77TEHRcq0XE } =; } 15, 42 -- X-YMail-OSG: mU9.CMYVM1nU5RWQzCOCo2tCXdcc5rBWDHvkrMrE8dmtNg2 } 15, 42 -- } ghcyqmxIav.mWZe55tGfPx8hB73Ku2Go_g2YcxIYVxYu.xIQcjRB8dOscnIl } 15, 42 -- ItGCm03ET3Xk9rdFmB5AyEOTu. } 5q67LQ3BVRuBgGI4Dyb1JBuFnhh6LX33Ge } 15, 42 -- } eZCSbcxsrxDfym2SvNF9HjWSjNSQhA3pmSisSwcORyEwAK7pVR6xPyjASZja } 15, 42 -- } 81UDvA.AJMLbz_lavTzjKSa7tsBX1HKarcZ_GAqFWj8E8vgim8xl_2cb9ajC } 15, 42 -- } J6x_Et5Oxs8J8PMTbE6fQbAsSz1rSnFYUn_0t2yB5pB4NvXbsBmtxHUE9IAq } 15, 42 -- } cGxGthxOtfOCAVTV5sIWmT0..AZylbKQ7UAE45AJjl9D4Pl9Kheo._ZrOqI7 } 15, 42 -- } 6JOY6X1VgqTVqTbHUdl2hNXyBKit3PZ5.7H4NFTszDoQ1wMaPo7PGZ7MJj6T } 15, 42 -- } ox4fhW2_ISZ4ykqYrKGhEE3K0h0_x9_whxj_V4afxT8ssY5stADMbOyv75Hf } 15, 42 -- } IV65YjtPjKc0RCBkjCk.JYib0q649_W.AcC2G7R7T0R_obP_lnUnSx67zPct } 15, 42 -- uAa96Geh729v3Ci.cpDJf6V93rfKrN65tq6cZtHoQ5c95q36LP3UHZ.u. } 7No } 15, 42 -- - } 15, 42 -- Received: from [80.122.101.100] by } web110815.mail.gq1.yahoo.com via HTTP; Mon, 06 Feb 2012 02:28:29 PST } 15, 42 -- X-Mailer: YahooMailWebService/0.8.116.338427 } 15, 42 -- References: {201202031910.q13JASuh012105-at-ns.microscopy.com} } 15, 42 -- Message-ID: } {1328524109.50274.YahooMailNeo-at-web110815.mail.gq1.yahoo.com } } } 15, 42 -- Date: Mon, 6 Feb 2012 02:28:29 -0800 (PST) 15, 42 -- From: } Stephane Nizet {nizets2-at-yahoo.com} 15, 42 -- Reply-To: Stephane Nizet } {nizets2-at-yahoo.com} 15, 42 -- Subject: Re: [Microscopy] TEM of } functionalized nanoparticles 15, 42 -- To: "marie.cantino-at-uconn.edu" } {marie.cantino-at-uconn.edu} 15, 42 -- Cc: "microscopy-at-microscopy.com" } {microscopy-at-microscopy.com} 15, 42 -- In-Reply-To: } {201202031910.q13JASuh012105-at-ns.microscopy.com} } 15, 42 -- MIME-Version: 1.0 } 15, 42 -- Content-Type: text/plain; charset=iso-8859-1 15, 42 -- } Content-Transfer-Encoding: 8bit 15, 42 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id q16ASUSx003271 } ==============================End of - } Headers==============================
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 9, 20 -- From marie.cantino-at-uconn.edu Mon Feb 6 08:40:11 2012 9, 20 -- Received: from mta1.uits.uconn.edu (smtp.uconn.edu [137.99.25.234]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16EeB9q015449 9, 20 -- for {microscopy-at-Microscopy.Com} ; Mon, 6 Feb 2012 08:40:11 -0600 9, 20 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9]) 9, 20 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id E24A83499C 9, 20 -- for {microscopy-at-Microscopy.Com} ; Mon, 6 Feb 2012 09:40:10 -0500 (EST) 9, 20 -- Message-Id: {E71EBD9F-CDF7-4B2B-A401-0FFA4339EE6D-at-uconn.edu} 9, 20 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 9, 20 -- To: microscopy-at-Microscopy.Com 9, 20 -- In-Reply-To: {201202061033.q16AXXWw011504-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 9, 20 -- Content-Transfer-Encoding: 7bit 9, 20 -- Mime-Version: 1.0 (Apple Message framework v936) 9, 20 -- Subject: Re: [Microscopy] Re: TEM of functionalized nanoparticles 9, 20 -- Date: Mon, 6 Feb 2012 09:40:10 -0500 9, 20 -- References: {201202061033.q16AXXWw011504-at-ns.microscopy.com} 9, 20 -- X-Mailer: Apple Mail (2.936) 9, 20 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 9, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 18, 34 -- From cdh1-at-cdc.gov Mon Feb 6 09:21:14 2012 18, 34 -- Received: from mail108.messagelabs.com (mail108.messagelabs.com [216.82.250.51]) 18, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16FLEVq012114 18, 34 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 09:21:14 -0600 18, 34 -- X-Env-Sender: cdh1-at-cdc.gov 18, 34 -- X-Msg-Ref: server-2.tower-108.messagelabs.com!1328541672!41620464!2 18, 34 -- X-Originating-IP: [158.111.54.24] 18, 34 -- X-StarScan-Version: 6.5.5; banners=-,-,- 18, 34 -- X-VirusChecked: Checked 18, 34 -- Received: (qmail 22741 invoked from network); 6 Feb 2012 15:21:13 -0000 18, 34 -- Received: from unknown (HELO ECASHUB-CLFT1.cdc.gov) (158.111.54.24) 18, 34 -- by server-2.tower-108.messagelabs.com with AES128-SHA encrypted SMTP; 6 Feb 2012 15:21:13 -0000 18, 34 -- Received: from EMBX-CLFT2.cdc.gov ([fe80::4cc3:f3c7:6959:3e75]) by 18, 34 -- ECASHUB-CLFT1.cdc.gov ([158.111.54.24]) with mapi id 14.01.0355.002; Mon, 6 18, 34 -- Feb 2012 10:21:12 -0500 18, 34 -- From: "Humphrey, Charles (CDC/OID/NCEZID)" {cdh1-at-cdc.gov} 18, 34 -- To: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} , 18, 34 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 18, 34 -- Subject: RE: [Microscopy] TEM of functionalized nanoparticles 18, 34 -- Thread-Topic: [Microscopy] TEM of functionalized nanoparticles 18, 34 -- Thread-Index: AQHM5N5xJ8ONW8kATku1XNgLH9uPRZYv9ohw 18, 34 -- Date: Mon, 6 Feb 2012 15:21:11 +0000 18, 34 -- Message-ID: {231981A6C34E2B4589ADAC40FC813A62182E9D91-at-EMBX-CLFT2.cdc.gov} 18, 34 -- References: {201202061448.q16Emm5i007737-at-ns.microscopy.com} 18, 34 -- In-Reply-To: {201202061448.q16Emm5i007737-at-ns.microscopy.com} 18, 34 -- Accept-Language: en-US 18, 34 -- Content-Language: en-US 18, 34 -- X-MS-Has-Attach: 18, 34 -- X-MS-TNEF-Correlator: 18, 34 -- x-originating-ip: [158.111.54.74] 18, 34 -- Content-Type: text/plain; charset="us-ascii" 18, 34 -- MIME-Version: 1.0 18, 34 -- Content-Transfer-Encoding: 8bit 18, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q16FLEVq012114 ==============================End of - Headers==============================
Image moving while focusing means that electron beam is not passing through the optical center of the lens. Either there is a problem with alignment electronics and it is pushed beam aside from its optimal path, or there is a problem with electron-optical elements inside of the column (maybe something got knocked out of alignment, or there is a large particle that got charged and distorted the field, or something else of similar nature).
In any case - look like you need to call a serviceman...
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/6/2012 9:08 AM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both ravi.thakkar369-at-gmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com Name: Ravindra Thakkar } } Title-Subject: [Filtered] TEM : Focusing Problem. } } Message: Image is not getting focused. The image is drastically moving } while focusing, it moving highly even at low focus step& at low } magnification. } } So how to resolve this problem. } } Login Host: 14.139.97.76 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Feb 6 08:08:03 2012 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16E83SK024773 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 08:08:03 -0600 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id 19AF8487506 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 08:08:03 -0600 (CST) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 8, 25 -- with ESMTP id 47U5ZZGt8Bky for {microscopy-at-microscopy.com} ; } 8, 25 -- Mon, 6 Feb 2012 08:07:55 -0600 (CST) } 8, 25 -- Received: from NestorPwrBk.local (dsl-58-6-20-170.wa.westnet.com.au [58.6.20.170]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id 5BFB34874F6 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 08:07:55 -0600 (CST) } 8, 25 -- Message-ID: {4F2FDEB8.7090909-at-microscopy.com} } 8, 25 -- Date: Mon, 06 Feb 2012 22:07:52 +0800 } 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: ravi.thakkar369-at-gmail.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: viaWWW:TEM : Focusing Problem. } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 39 -- From vray-at-partbeamsystech.com Mon Feb 6 09:46:38 2012 4, 39 -- Received: from nm4-vm0.access.bullet.mail.mud.yahoo.com (nm4-vm0.access.bullet.mail.mud.yahoo.com [66.94.237.138]) 4, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q16Fkc8o029432 4, 39 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 09:46:38 -0600 4, 39 -- Received: from [66.94.237.196] by nm4.access.bullet.mail.mud.yahoo.com with NNFMP; 06 Feb 2012 15:46:38 -0000 4, 39 -- Received: from [98.138.207.11] by tm7.access.bullet.mail.mud.yahoo.com with NNFMP; 06 Feb 2012 15:46:37 -0000 4, 39 -- Received: from [127.0.0.1] by smtp104.biz.mail.ne1.yahoo.com with NNFMP; 06 Feb 2012 15:46:37 -0000 4, 39 -- X-Yahoo-Newman-Id: 837894.89936.bm-at-smtp104.biz.mail.ne1.yahoo.com 4, 39 -- X-Yahoo-Newman-Property: ymail-3 4, 39 -- X-YMail-OSG: sRSCRgQVM1lv3Vc9BTDOSB1OfDQ9322eUDYhTOO.UYeV0V1 4, 39 -- 7WhRXbueMTBGCPy.VhtJhaT64W4s7hFES1FXQD9NTjhp.Qo.7cLcKdlw7kWo 4, 39 -- ranKF.fJBBkGtiSee7sIMZE9sPCE0wXq77oFxZ.ttJHaWYl930Vsa9PyKiaS 4, 39 -- AUhUCOCiy7IWihO93VneQL44vniHu1_EMU_WPtL1URBhzTaNonLFT_A9kJ9g 4, 39 -- cjsAIVVCFcm_w2hUmUV3JqMX7JhS83wb7HScKrhulARXFZ8VbWqRR9eiNMsh 4, 39 -- Hx3a.In3Hr6yCG3qnXd9QKtXQqB98u6hNSV3EQq8HP9LH6jsoi60PafeNveG 4, 39 -- p_Hxi_pf9QfYfS10hjEVze_X5_S9vOzrhbQmS7uo_j7ch1DwrHZJViDVq3Bl 4, 39 -- V2Qpw9_9uE.MC3urWaXoPSVL_f7FwYGpTjfzgTU0lLm6QBe24hxpFZ4_PoKX 4, 39 -- IpO1IBn_H3UUtZD8E2Qikdjpmz7sc4ZtGmaVM8oyZ3kElqTVv6Fk4_DCaIcy 4, 39 -- GEA7PZE3diAPezXO81hXRcuCNl_kFb0NAc4b0qVASPM0UYM3brMLQTk.7HMj 4, 39 -- uYeJs9SstwXk8g28Jyb7OgiaI5PDoU73eZhHeb9IYcaUXRcGgg6iTLoii9BO 4, 39 -- w2nu_AWIXm3.genFPFoxkxIcGiw71JKvcgIF6xNeH80UHpTIQFlrD_zqKN1X 4, 39 -- eAyAYqkU7WR5iWgCvzE3a9MKxS5EIk9Ryin2EqQ2zJ.vdFLehayiiRPnap9z 4, 39 -- ChE5.ZTdl6y_a291nTHtQdeCZOT4- 4, 39 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 39 -- Received: from [10.109.165.244] (vray-at-129.2.129.153 with plain) 4, 39 -- by smtp104.biz.mail.ne1.yahoo.com with SMTP; 06 Feb 2012 07:46:37 -0800 PST 4, 39 -- Message-ID: {4F2FF5DD.5010502-at-partbeamsystech.com} 4, 39 -- Date: Mon, 06 Feb 2012 10:46:37 -0500 4, 39 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 4, 39 -- Reply-To: vray-at-partbeamsystech.com 4, 39 -- Organization: PBS&T 4, 39 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:9.0) Gecko/20111222 Thunderbird/9.0.1 4, 39 -- MIME-Version: 1.0 4, 39 -- To: microscopy-at-microscopy.com, ravi.thakkar369-at-gmail.com 4, 39 -- Subject: Re: [Microscopy] viaWWW:TEM : Focusing Problem. 4, 39 -- References: {201202061408.q16E8r97026059-at-ns.microscopy.com} 4, 39 -- In-Reply-To: {201202061408.q16E8r97026059-at-ns.microscopy.com} 4, 39 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
One thing to look for in the electronics readout system (perhaps a computer with memory) is the alignment numbers, giving gun alignment numbers (two sets for X and two sets of Y) and the condenser alignment (likewise) and perhaps Projector alignment, even image shift on some models. Basically you would love to have a set of "known good" numbers for reference. By comparing the last known good alignment numbers with the present numbers you can eliminate the possibility of the previous user being rather too keen to perform 'dark field' experiments.
As previous posters mention it is the condenser lens (CL) TILT alignment that might be in error, another possibility is that the sample is bent horribly and the lens is focusing at a value that is nowhere near it's optimal value (have you checked the eucentric height?). Again having values of lens currents recorded after a good session can be useful and many service people do just that, by checking the performance report from the installation.
Is the Gun tilt (filament image), condenser aperture centering, and spot size adjustments all perfectly normal?
Good luck, Rob Keyse
On 2/6/2012 10:53 AM, vray-at-partbeamsystech.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Image moving while focusing means that electron beam is not passing } through the optical center of the lens. Either there is a problem with } alignment electronics and it is pushed beam aside from its optimal path, } or there is a problem with electron-optical elements inside of the } column (maybe something got knocked out of alignment, or there is a } large particle that got charged and distorted the field, or something } else of similar nature). } } In any case - look like you need to call a serviceman... } } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 - leave a message with call-back number } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 2/6/2012 9:08 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying please copy both ravi.thakkar369-at-gmail.com as well as the } } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: ravi.thakkar369-at-gmail.com Name: Ravindra Thakkar } } } } Title-Subject: [Filtered] TEM : Focusing Problem. } } } } Message: Image is not getting focused. The image is drastically moving } } while focusing, it moving highly even at low focus step& at low } } magnification. } } } } So how to resolve this problem. } } } } Login Host: 14.139.97.76 } } --------------------------------------------------------------------------- } }
-- Robert Keyse EM Facility Whitaker Laboratory 5 East Packer Avenue Bethlehem PA 18015 USA
Tel. +1 610 758 4283 Fax +1 610 758 4244
==============================Original Headers============================== 8, 24 -- From rok210-at-lehigh.edu Mon Feb 6 10:05:31 2012 8, 24 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16G5VSV014365 8, 24 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 10:05:31 -0600 8, 24 -- Received: from [127.0.0.1] (Dyn055015.mat.Lehigh.EDU [128.180.55.15]) 8, 24 -- (authenticated bits=0) 8, 24 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id q16G5U6V025821 8, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 24 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 11:05:30 -0500 8, 24 -- Message-ID: {4F2FFA4A.9010201-at-lehigh.edu} 8, 24 -- Date: Mon, 06 Feb 2012 11:05:30 -0500 8, 24 -- From: Robert Keyse {rok210-at-lehigh.edu} 8, 24 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:10.0) Gecko/20120129 Thunderbird/10.0 8, 24 -- MIME-Version: 1.0 8, 24 -- To: microscopy-at-microscopy.com 8, 24 -- Subject: Re: [Microscopy] Re: viaWWW:TEM : Focusing Problem. 8, 24 -- References: {201202061553.q16FrTtP011127-at-ns.microscopy.com} 8, 24 -- In-Reply-To: {201202061553.q16FrTtP011127-at-ns.microscopy.com} 8, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 24 -- Content-Transfer-Encoding: 7bit 8, 24 -- X-Antivirus: avast! (VPS 120206-0, 02/06/2012), Outbound message 8, 24 -- X-Antivirus-Status: Clean 8, 24 -- X-Virus-Scanned: clamav-milter 0.97.3 at rain.cc.lehigh.edu 8, 24 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Hello Marie, I agree with Charles: "you should be able to see IgG .... by negative staining". However, I have never used negative staining for samples containing colloidal gold particles bigger than 10 nm. In my hand (~5 to ~10 nm gold particles on thin carbon support film), 1% or 2% ammonium molybdate or its mixture with 0.1% trehalose worked better than classic 2% uranyl acetate. Meniscus of uranyl acetate around the gold particle was usually too dense to see IgG attached to it at 80 kV and tungsten cathode.
Best regards Oldrich
Prague Czech Republic
On Monday 06 of February 2012 16:23:06 you wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } Hello Marie, } } I am not certain this answers your question but you should be able to } detect IgG attached to an electron dense gold particle by negative stain } EM. Antibodies are commonly seen attached to virus particles and to } colloidal gold particles when negative stain EM is done. Anything you } would likely see in your indicated study by AFM can be seen by negative } stain EM. All techniques have various artifacts including light-scatter, } thin-section EM, AFM, FESEM, and cryo-EM. The key to EM and techniques in } general is to learn what is real and what is artifact. It often takes } time and work to develop proficiency with many techniques though. Good } luck with this , it should be possible. } } Charles Humphrey, Ph. D. } CDC } Atlanta, GA 30333 } } -----Original Message----- } X-from: marie.cantino-at-uconn.edu [mailto:marie.cantino-at-uconn.edu] } Sent: Monday, February 06, 2012 9:49 AM } To: Humphrey, Charles (CDC/OID/NCEZID) } Subject: [Microscopy] TEM of functionalized nanoparticles } } } } } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } Hi Stefan, } } I do understand the difference between mass and length. } } The problem is that the clients usually can only give us the molecular } weight and (if we're lucky) the number of molecules per particle. If they } knew how the proteins/peptides were arranged or the linear dimensions of } the coating they wouldn't need to come to me. What I'm trying to find out } from the listserver or the literature is whether we can reasonably expect } to be able to detect a medium sized protein such as IgG on an electron } dense gold particle, given the various artifacts of techniques such as } negative staining. } } We only have TEM (w/a tungsten emitter) and FESEM in our facility, but I } can send them elsewhere for AFM. } } Marie } } On Feb 6, 2012, at 5:33 AM, nizets2-at-yahoo.com wrote: } } ---------------------------------------------------------------------- } } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } Hi Marie, } } } } I like these projects which put the techniques to their limits. } } I think the reason why you have difficulties finding publications on } } this matter and that the question is not trivial at all. } } First of all, and to start with a relaxed note, the mixing of kDa und } } nm in one sentence made me smile because it makes as much sense as } } asking if 1kg of something fits in one meter. } } The Dalton is a unit of mass and does not give a good indication of } } the dimension or the shape of a molecule. } } That said, I seem to remember from the past answers of our emminent } } immunoglobulinologist Jan Leunissen that the size of an IgG is several } } nm, I think more than 5nm. } } Which means, in your case, that you should not expect more that a few } } molecules per nanoparticle, except if they form multilayers. } } As for the techniques, I wonder if a SEM could resolve this structure. } } Although I have experience with analytical low res SEMs, I don't know } } the limits of modern FEG SEMs. } } I would be happy if some listers would care to share their opinion on } } this matter. } } It seems that AFM (atomic force microscopy) would be able to resolve } } this structure, but I wonder if it would be possible in this condition } } because AFM works on flat substrate and here you have round particles. } } It would probably be possible to find some answers using cry- } } microscopy, the same way scientists use it to resolve viral structures } } at several anstrom resolution. It probably depends on how much time } } and money your clients are wanting to spend and how critical this } } information is for them. } } } } Regards, } } Stephane } } } } ----- Original Message ----- } } X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} } } To: nizets2-at-yahoo.com } } Cc: } } Sent: Friday, February 3, 2012 8:10 PM } } Subject: [Microscopy] TEM of functionalized nanoparticles } } } } } } } } } } ---------------------------------------------------------------------- } } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } In recent years we have been getting requests to image functionalized } } nanoparticles, such as 10 nm gold with proteins or peptides attached. } } Generally the clients want to know how thick or large the organic } } "shell" is, or how the protein/peptide is arranged on the surface. } } Most of our experience has been with either inorganic particles (which } } we view without staining) or larger organic structures such as viral } } procapsids or large proteins (e.g., myosin) or protein assemblies, } } which we negative stain. My questions for this list are as follows: } } } } - have others on the list had good luck imaging these kinds of } } conjugates at the EM level, and if so, using what techniques? } } - how much organic material must one have associated with the } } nanoparticle to see it by negative staining or other methods? For } } example, how hard is it to detect a 150 kD protein on a 10 nm gold } } particle? An example would be IgG attached to a 10 nm gold particle. } } - can you suggest any publications or review articles on the subject? } } For some reason I'm not having much luck with my Pubmed search } } strategies. A recent paper by Cao and Mao seems to deal mainly with } } small gold particles on much larger protein aggregates, but perhaps } } there are others out there. } } } } Many thanks (in advance) for your suggestions. } } } } Dr. Marie E. Cantino } } Associate Professor of Physiology and Neurobiology Director, Electron } } Microscopy Laboratory University of Connecticut, Unit 3242 } } Phone: 860-486-3588 } } Fax: 860-486-6369 } } } } } } ==============================Original } } Headers============================== } } 5, 18 -- From marie.cantino-at-uconn.edu Fri Feb 3 13:07:15 2012 5, 18 } } -- Received: from mta1.uits.uconn.edu (mailscanner.uconn.edu } } [137.99.25.234]) } } 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id q13J7FK2006567 } } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 } } 13:07:15 -0600 } } 5, 18 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu } } [137.99.47.9]) } } 5, 18 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 4D02634B9A } } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 } } 14:07:15 -0500 (EST) } } 5, 18 -- Message-Id: {6803418B-8131-4A98-990F-158505836E6E-at-uconn.edu} } } 5, 18 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 5, 18 -- To: } } Microscopy-at-Microscopy.Com 5, 18 -- Content-Type: text/plain; } } charset=US-ASCII; format=flowed; delsp=yes 5, 18 -- } } Content-Transfer-Encoding: 7bit 5, 18 -- Mime-Version: 1.0 (Apple } } Message framework v936) 5, 18 -- Subject: TEM of functionalized } } nanoparticles 5, 18 -- Date: Fri, 3 Feb 2012 14:07:15 -0500 5, 18 -- } } X-Mailer: Apple Mail (2.936) 5, 18 -- X-Virus-Scanned: clamav-milter } } 0.96.5 at mta1 5, 18 -- X-Virus-Status: Clean } } ==============================End of - } } Headers============================== } } } } } } } } ==============================Original } } Headers============================== } } 15, 42 -- From nizets2-at-yahoo.com Mon Feb 6 04:28:31 2012 15, 42 -- } } Received: from nm9.bullet.mail.sp2.yahoo.com } } (nm9.bullet.mail.sp2.yahoo.com [98.139.91.79]) } } 15, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } id q16ASUSx003271 } } 15, 42 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 04:28:31 } } -0600 } } 15, 42 -- Received: from [98.139.91.62] by } } nm9.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 } } 15, 42 -- Received: from [98.139.91.23] by } } tm2.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 } } 15, 42 -- Received: from [127.0.0.1] by omp1023.mail.sp2.yahoo.com } } with NNFMP; 06 Feb 2012 10:28:30 -0000 15, 42 -- } } X-Yahoo-Newman-Property: ymail-3 15, 42 -- X-Yahoo-Newman-Id: } } 481221.57336.bm-at-omp1023.mail.sp2.yahoo.com } } 15, 42 -- Received: (qmail 64306 invoked by uid 60001); 6 Feb 2012 } } 10:28:30 -0000 } } 15, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } d=yahoo.com; s=s1024; t=1328524109; bh= } } +xZoCP0S7BlCXixrJBv6fCBgmv9JLm9A9772ftf9y9w=; h=X-YMail- } } OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply- } } To:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content- } } Transfer-Encoding; } } b } } = } } DhiSJ4sQkcV0vFmz7NomZjwgCjpBj4FAdn7ZD2wksosyYhu8N5W2UqbTQyeXCJK4oVawc6 } } 9mSzTPOiPLJMDjlVLrxuWj } } +2HE7+negf7O2gV72PfH1xIgxl0oKW5yjsdKajsyvKAsKy1AyxjksZ4FKsRyicVW6P } } +XS3bODk3UHBI= } } 15, 42 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } } 15, 42 -- s=s1024; d=yahoo.com; } } 15, 42 -- h=X-YMail-OSG:Received:X-Mailer:References:Message- } } ID:Date:From:Reply-To:Subject:To:Cc:In-Reply-To:MIME-Version:Content- } } Type:Content-Transfer-Encoding; } } 15, 42 -- b=0z5d16psGKpudCX/OEBR6HSJZodETUvunNzVkE2Bdt/ } } 50FwYt985UVUxt } } + } } wGQv0dJpC6PSS5C1UVuucgfGoIke8emqTSu5ngf9h5IlUbvg19SDLYgnCq56YYozOelWUh } } S7ZeiXK6XJoArVPn97DAVKnn36IdAtBG77TEHRcq0XE } } =; } } 15, 42 -- X-YMail-OSG: mU9.CMYVM1nU5RWQzCOCo2tCXdcc5rBWDHvkrMrE8dmtNg2 } } 15, 42 -- } } ghcyqmxIav.mWZe55tGfPx8hB73Ku2Go_g2YcxIYVxYu.xIQcjRB8dOscnIl } } 15, 42 -- ItGCm03ET3Xk9rdFmB5AyEOTu. } } 5q67LQ3BVRuBgGI4Dyb1JBuFnhh6LX33Ge } } 15, 42 -- } } eZCSbcxsrxDfym2SvNF9HjWSjNSQhA3pmSisSwcORyEwAK7pVR6xPyjASZja } } 15, 42 -- } } 81UDvA.AJMLbz_lavTzjKSa7tsBX1HKarcZ_GAqFWj8E8vgim8xl_2cb9ajC } } 15, 42 -- } } J6x_Et5Oxs8J8PMTbE6fQbAsSz1rSnFYUn_0t2yB5pB4NvXbsBmtxHUE9IAq } } 15, 42 -- } } cGxGthxOtfOCAVTV5sIWmT0..AZylbKQ7UAE45AJjl9D4Pl9Kheo._ZrOqI7 } } 15, 42 -- } } 6JOY6X1VgqTVqTbHUdl2hNXyBKit3PZ5.7H4NFTszDoQ1wMaPo7PGZ7MJj6T } } 15, 42 -- } } ox4fhW2_ISZ4ykqYrKGhEE3K0h0_x9_whxj_V4afxT8ssY5stADMbOyv75Hf } } 15, 42 -- } } IV65YjtPjKc0RCBkjCk.JYib0q649_W.AcC2G7R7T0R_obP_lnUnSx67zPct } } 15, 42 -- uAa96Geh729v3Ci.cpDJf6V93rfKrN65tq6cZtHoQ5c95q36LP3UHZ.u. } } 7No } } 15, 42 -- - } } 15, 42 -- Received: from [80.122.101.100] by } } web110815.mail.gq1.yahoo.com via HTTP; Mon, 06 Feb 2012 02:28:29 PST } } 15, 42 -- X-Mailer: YahooMailWebService/0.8.116.338427 } } 15, 42 -- References: {201202031910.q13JASuh012105-at-ns.microscopy.com} } } 15, 42 -- Message-ID: } } {1328524109.50274.YahooMailNeo-at-web110815.mail.gq1.yahoo.com } } } } 15, 42 -- Date: Mon, 6 Feb 2012 02:28:29 -0800 (PST) 15, 42 -- From: } } Stephane Nizet {nizets2-at-yahoo.com} 15, 42 -- Reply-To: Stephane Nizet } } {nizets2-at-yahoo.com} 15, 42 -- Subject: Re: [Microscopy] TEM of } } functionalized nanoparticles 15, 42 -- To: "marie.cantino-at-uconn.edu" } } {marie.cantino-at-uconn.edu} 15, 42 -- Cc: "microscopy-at-microscopy.com" } } {microscopy-at-microscopy.com} 15, 42 -- In-Reply-To: } } {201202031910.q13JASuh012105-at-ns.microscopy.com} } } 15, 42 -- MIME-Version: 1.0 } } 15, 42 -- Content-Type: text/plain; charset=iso-8859-1 15, 42 -- } } Content-Transfer-Encoding: 8bit 15, 42 -- X-MIME-Autoconverted: from } } quoted-printable to 8bit by ns.microscopy.com id q16ASUSx003271 } } ==============================End of - } } Headers============================== } } Dr. Marie E. Cantino } Associate Professor of Physiology and Neurobiology Director, Electron } Microscopy Laboratory University of Connecticut, Unit 3242 Phone: } 860-486-3588 } Fax: 860-486-6369 } } } ==============================Original } Headers============================== 9, 20 -- From } marie.cantino-at-uconn.edu Mon Feb 6 08:40:11 2012 9, 20 -- Received: from } mta1.uits.uconn.edu (smtp.uconn.edu [137.99.25.234]) 9, 20 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16EeB9q015449 } 9, 20 -- for {microscopy-at-Microscopy.Com} ; Mon, 6 Feb 2012 08:40:11 -0600 } 9, 20 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu } [137.99.47.9]) 9, 20 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id } E24A83499C 9, 20 -- for {microscopy-at-Microscopy.Com} ; Mon, 6 Feb 2012 } 09:40:10 -0500 (EST) 9, 20 -- Message-Id: } {E71EBD9F-CDF7-4B2B-A401-0FFA4339EE6D-at-uconn.edu} 9, 20 -- From: Marie } Cantino {marie.cantino-at-uconn.edu} 9, 20 -- To: microscopy-at-Microscopy.Com } 9, 20 -- In-Reply-To: {201202061033.q16AXXWw011504-at-ns.microscopy.com} 9, } 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 9, 20 -- Content-Transfer-Encoding: 7bit 9, 20 -- Mime-Version: 1.0 (Apple } Message framework v936) 9, 20 -- Subject: Re: [Microscopy] Re: TEM of } functionalized nanoparticles 9, 20 -- Date: Mon, 6 Feb 2012 09:40:10 -0500 } 9, 20 -- References: {201202061033.q16AXXWw011504-at-ns.microscopy.com} 9, 20 } -- X-Mailer: Apple Mail (2.936) } 9, 20 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 9, 20 -- } X-Virus-Status: Clean ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== 18, 34 -- From cdh1-at-cdc.gov Mon Feb } 6 09:21:14 2012 } 18, 34 -- Received: from mail108.messagelabs.com (mail108.messagelabs.com } [216.82.250.51]) 18, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id q16FLEVq012114 18, 34 -- for {microscopy-at-microscopy.com} ; } Mon, 6 Feb 2012 09:21:14 -0600 18, 34 -- X-Env-Sender: cdh1-at-cdc.gov } 18, 34 -- X-Msg-Ref: } server-2.tower-108.messagelabs.com!1328541672!41620464!2 18, 34 -- } X-Originating-IP: [158.111.54.24] } 18, 34 -- X-StarScan-Version: 6.5.5; banners=-,-,- } 18, 34 -- X-VirusChecked: Checked } 18, 34 -- Received: (qmail 22741 invoked from network); 6 Feb 2012 15:21:13 } -0000 18, 34 -- Received: from unknown (HELO ECASHUB-CLFT1.cdc.gov) } (158.111.54.24) 18, 34 -- by server-2.tower-108.messagelabs.com with } AES128-SHA encrypted SMTP; 6 Feb 2012 15:21:13 -0000 18, 34 -- Received: } from EMBX-CLFT2.cdc.gov ([fe80::4cc3:f3c7:6959:3e75]) by 18, 34 -- } ECASHUB-CLFT1.cdc.gov ([158.111.54.24]) with mapi id 14.01.0355.002; Mon, } 6 18, 34 -- Feb 2012 10:21:12 -0500 } 18, 34 -- From: "Humphrey, Charles (CDC/OID/NCEZID)" {cdh1-at-cdc.gov} } 18, 34 -- To: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} , } 18, 34 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 18, 34 -- Subject: RE: [Microscopy] TEM of functionalized nanoparticles } 18, 34 -- Thread-Topic: [Microscopy] TEM of functionalized nanoparticles } 18, 34 -- Thread-Index: AQHM5N5xJ8ONW8kATku1XNgLH9uPRZYv9ohw } 18, 34 -- Date: Mon, 6 Feb 2012 15:21:11 +0000 } 18, 34 -- Message-ID: } {231981A6C34E2B4589ADAC40FC813A62182E9D91-at-EMBX-CLFT2.cdc.gov} 18, 34 -- } References: {201202061448.q16Emm5i007737-at-ns.microscopy.com} 18, 34 -- } In-Reply-To: {201202061448.q16Emm5i007737-at-ns.microscopy.com} 18, 34 -- } Accept-Language: en-US } 18, 34 -- Content-Language: en-US } 18, 34 -- X-MS-Has-Attach: } 18, 34 -- X-MS-TNEF-Correlator: } 18, 34 -- x-originating-ip: [158.111.54.74] } 18, 34 -- Content-Type: text/plain; charset="us-ascii" } 18, 34 -- MIME-Version: 1.0 } 18, 34 -- Content-Transfer-Encoding: 8bit } 18, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id q16FLEVq012114 ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 40 -- From benada-at-biomed.cas.cz Mon Feb 6 10:33:21 2012 4, 40 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16GXKo3031902 4, 40 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 10:33:20 -0600 4, 40 -- X-ASG-Debug-ID: 1328545996-011d813302b45270001-4CH8be 4, 40 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id C6QeCfZ1y2CIfwpk (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Mon, 06 Feb 2012 17:33:16 +0100 (CET) 4, 40 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 4, 40 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 4, 40 -- Received: from u117ob.localnet (unknown [147.231.44.252]) 4, 40 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 4, 40 -- (No client certificate requested) 4, 40 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 5B7B92C816B 4, 40 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 17:33:16 +0100 (CET) 4, 40 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 4, 40 -- Organization: =?utf-8?q?Mikrobiologick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?=, v.v.i. 4, 40 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 4, 40 -- Subject: Re: [Microscopy] RE: TEM of functionalized nanoparticles 4, 40 -- Date: Mon, 6 Feb 2012 17:33:01 +0100 4, 40 -- X-ASG-Orig-Subj: Re: [Microscopy] RE: TEM of functionalized nanoparticles 4, 40 -- User-Agent: KMail/1.13.7 (Linux/3.1.0-1-686-pae; KDE/4.6.5; i686; ; ) 4, 40 -- References: {201202061523.q16FN6ij015289-at-ns.microscopy.com} 4, 40 -- In-Reply-To: {201202061523.q16FN6ij015289-at-ns.microscopy.com} 4, 40 -- MIME-Version: 1.0 4, 40 -- Message-Id: {201202061733.01611.benada-at-biomed.cas.cz} 4, 40 -- Content-Type: Text/Plain; 4, 40 -- charset="us-ascii" 4, 40 -- Content-Transfer-Encoding: 7bit 4, 40 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 4, 40 -- X-Barracuda-Start-Time: 1328545996 4, 40 -- X-Barracuda-Encrypted: AES256-SHA 4, 40 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 4, 40 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 4, 40 -- X-Barracuda-Spam-Score: 0.82 4, 40 -- X-Barracuda-Spam-Status: No, SCORE=0.82 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 tests=BSF_RULE7568M, URI_HEX 4, 40 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.87786 4, 40 -- Rule breakdown below 4, 40 -- pts rule name description 4, 40 -- ---- ---------------------- -------------------------------------------------- 4, 40 -- 0.32 URI_HEX URI: URI hostname has long hexadecimal sequence 4, 40 -- 0.50 BSF_RULE7568M Custom Rule 7568M ==============================End of - Headers==============================
Hopefully you are not situated in the area of floodings!
Concerning your question(s) below I would like to point you to a MSA- Listserver-thread (2007) with -/+ such background (a compilation of the postings I could send to you relatively fast...but there would be also other interesting questions and answers (which one can find also in recent and a little bit "older" specific literature (from experts) on that matter. So, please, if you would like to get a compilation of the 2007-MSA-LS- thread (instead of searching for them in the archives) please let me know (and "allow" for sending to your mailbox...),
for now, best regards, Wolfgang MUSS
} SALZBURG, AUSTRIA } } } -----Ursprüngliche Nachricht----- } } Von: microscopylistserver-noreply-at-microscopy.com } } [mailto:microscopylistserver-noreply-at-microscopy.com] } } Gesendet: Freitag, 03. Februar 2012 16:40 } } An: Muß Wolfgang } } Betreff: [Microscopy] Counting virus particles } } } --------------------------------------------------------------------- } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } ------- } } This Question/Comment was submitted to the Microscopy Listserver } using the } } WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------- } ------ } } Remember this posting is most likely not from a Subscriber, so when } } replying please copy both mary.walker-at-csl.com.au as well as the } } MIcroscopy Listserver } } --------------------------------------------------------------------- } ------ } } Email: mary.walker-at-csl.com.au } } Name: mary walker } } Organization: csl limited } } Title-Subject: counting virus particles } } Message: } } Do any listers have experience with counting virus particles with } latex } } beads? } } Is it possible to do better than order of magnitude? } } What is the minimum number of particles per ml that can be counted? } } What is the lower limit? } } } } thanks in advance } } } } Login Host: 203.10.36.68 } } --------------------------------------------------------------------- } ------ } } } } ==============================Original } Headers============================== } } 9, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 3 } } 09:35:54 2012 } } 9, 25 -- Received: from znl.com ([206.69.208.20]) } } 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id q13FZs77015955 } } 9, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 09:35:54 - } } 0600 } } 9, 25 -- Received: from localhost (localhost [127.0.0.1]) } } 9, 25 -- by znl.com (Postfix) with ESMTP id 9B984485144 } } 9, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 09:35:54 } } -0600 (CST) } } 9, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } } 9, 25 -- Received: from znl.com ([127.0.0.1]) } } 9, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd- } } new, port 10024) } } 9, 25 -- with ESMTP id GA2KYCBcTT6V for {microscopy-at-microscopy.com} ; } } 9, 25 -- Fri, 3 Feb 2012 09:35:54 -0600 (CST) } } 9, 25 -- Received: from NestorPwrBk.local (dsl-58-6-4- } } 117.wa.westnet.com.au [58.6.4.117]) } } 9, 25 -- by znl.com (Postfix) with ESMTPA id 858CD485134 } } 9, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2012 09:35:53 } } -0600 (CST) } } 9, 25 -- Message-ID: {4F2BFED4.7070106-at-microscopy.com} } } 9, 25 -- Date: Fri, 03 Feb 2012 23:35:48 +0800 } } 9, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver- } } noreply-at-microscopy.com} } } 9, 25 -- Reply-To: mary.walker-at-csl.com.au } } 9, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } } 9, 25 -- MIME-Version: 1.0 } } 9, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 9, 25 -- Subject: viaWWW:counting virus particles } } 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 9, 25 -- Content-Transfer-Encoding: 7bit } } ==============================End of - } } Headers==============================
==============================Original Headers============================== 9, 37 -- From W.Muss-at-salk.at Mon Feb 6 11:34:15 2012 9, 37 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 9, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16HYF9D017852 9, 37 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 11:34:15 -0600 9, 37 -- Received: from localhost (localhost [127.0.0.1]) 9, 37 -- by hermes.salk.at (Postfix) with ESMTP id 0BD9AC3846 9, 37 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 18:33:50 +0100 (CET) 9, 37 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 9, 37 -- Received: from hermes.salk.at ([127.0.0.1]) 9, 37 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 9, 37 -- with ESMTP id jggEIJxY7T9w for {microscopy-at-microscopy.com} ; 9, 37 -- Mon, 6 Feb 2012 18:33:49 +0100 (CET) 9, 37 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 9, 37 -- by hermes.salk.at (Postfix) with ESMTP id A48ADC383D 9, 37 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 18:33:49 +0100 (CET) 9, 37 -- Received: from n1rz122.lksdom21.lks.local ([192.168.101.122]) 9, 37 -- by N2EX199.lks.local with ESMTP; 06 Feb 2012 18:35:27 +0100 9, 37 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.133]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.4675); 9, 37 -- Mon, 6 Feb 2012 18:34:13 +0100 9, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 37 -- Content-class: urn:content-classes:message 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Type: text/plain; 9, 37 -- charset="iso-8859-1" 9, 37 -- Subject: [Microscopy] Re: Counting virus particles 9, 37 -- Date: Mon, 6 Feb 2012 18:34:10 +0100 9, 37 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062F33608-at-N1RZ116.lksdom21.lks.local} 9, 37 -- X-MS-Has-Attach: 9, 37 -- X-MS-TNEF-Correlator: 9, 37 -- Thread-Topic: [Microscopy] Re: Counting virus particles 9, 37 -- Thread-Index: AcziiiIP0uSg8ptmQFuDUqsmWgcYdgCMZVLAAA5lr/A= 9, 37 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 9, 37 -- To: {microscopy-at-microscopy.com} 9, 37 -- X-OriginalArrivalTime: 06 Feb 2012 17:34:13.0781 (UTC) FILETIME=[8B0B8450:01CCE4F5] 9, 37 -- X-Scanned-By: SALK-Content-Filter 9, 37 -- Content-Transfer-Encoding: 8bit 9, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q16HYF9D017852 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both csrayat-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: csrayat-at-gmail.com Name: Dr CS Rayat
Organization: PGIMER, Chandigarh, India
Title-Subject: [Filtered] Service Manual_Zeiss EM-906
Message: Hi Friends !
Please e-mail me a copy (pdf format) of Service Manual of Zeiss EM-906 (Transmission Electron Microscope) or Trouble Shooting Manual for the same. Best regards,
Somewhere in the 90's I believe I read an abstract that showed excellent negative staining of gold particles attached to immunoglobulin. Unfortunately, I do not have access to that literature here so I can not help you with a reference. If I remember correctly in those studies first the Ig component was a adsorbed onto a grid film which was then, after rinsing incubated with the gold particles to study the way those particles bound and negatively stained. I believe these were proceedings from one of the international EM conferences, perhaps someone else has access?
Good luck,
Jan Leunissen
e: http://www.aurion.nl
On 4/02/2012, at 8:07 AM, marie.cantino-at-uconn.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } In recent years we have been getting requests to image functionalized } nanoparticles, such as 10 nm gold with proteins or peptides attached. } Generally the clients want to know how thick or large the organic } "shell" is, or how the protein/peptide is arranged on the surface. } Most of our experience has been with either inorganic particles (which } we view without staining) or larger organic structures such as viral } procapsids or large proteins (e.g., myosin) or protein assemblies, } which we negative stain. My questions for this list are as follows: } } - have others on the list had good luck imaging these kinds of } conjugates at the EM level, and if so, using what techniques? } - how much organic material must one have associated with the } nanoparticle to see it by negative staining or other methods? For } example, how hard is it to detect a 150 kD protein on a 10 nm gold } particle? An example would be IgG attached to a 10 nm gold particle. } - can you suggest any publications or review articles on the subject? } For some reason I'm not having much luck with my Pubmed search } strategies. A recent paper by Cao and Mao seems to deal mainly with } small gold particles on much larger protein aggregates, but perhaps } there are others out there. } } Many thanks (in advance) for your suggestions. } } Dr. Marie E. Cantino } Associate Professor of Physiology and Neurobiology } Director, Electron Microscopy Laboratory } University of Connecticut, Unit 3242 } Phone: 860-486-3588 } Fax: 860-486-6369 } } } ==============================Original Headers============================== } 5, 18 -- From marie.cantino-at-uconn.edu Fri Feb 3 13:07:15 2012 } 5, 18 -- Received: from mta1.uits.uconn.edu (mailscanner.uconn.edu [137.99.25.234]) } 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13J7FK2006567 } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 13:07:15 -0600 } 5, 18 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9]) } 5, 18 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 4D02634B9A } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 14:07:15 -0500 (EST) } 5, 18 -- Message-Id: {6803418B-8131-4A98-990F-158505836E6E-at-uconn.edu} } 5, 18 -- From: Marie Cantino {marie.cantino-at-uconn.edu} } 5, 18 -- To: Microscopy-at-Microscopy.Com } 5, 18 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 5, 18 -- Content-Transfer-Encoding: 7bit } 5, 18 -- Mime-Version: 1.0 (Apple Message framework v936) } 5, 18 -- Subject: TEM of functionalized nanoparticles } 5, 18 -- Date: Fri, 3 Feb 2012 14:07:15 -0500 } 5, 18 -- X-Mailer: Apple Mail (2.936) } 5, 18 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 } 5, 18 -- X-Virus-Status: Clean } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 21 -- From leunissen-at-aurion.nl Mon Feb 6 16:25:04 2012 10, 21 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz [210.54.141.244]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16MP3HZ029504 10, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 16:25:04 -0600 10, 21 -- Received: from jan-desktop.lan ([122.57.251.172]) by fep02.xtra.co.nz 10, 21 -- with ESMTP 10, 21 -- id {20120206222454.YIQZ15970.fep02.xtra.co.nz-at-jan-desktop.lan} ; 10, 21 -- Tue, 7 Feb 2012 11:24:54 +1300 10, 21 -- Subject: Re: [Microscopy] TEM of functionalized nanoparticles 10, 21 -- Mime-Version: 1.0 (Apple Message framework v1084) 10, 21 -- Content-Type: text/plain; charset=us-ascii 10, 21 -- From: Jan Leunissen {leunissen-at-aurion.nl} 10, 21 -- In-Reply-To: {201202031907.q13J7Q5T006851-at-ns.microscopy.com} 10, 21 -- Date: Tue, 7 Feb 2012 11:24:53 +1300 10, 21 -- Cc: Microscopy-at-microscopy.com 10, 21 -- Message-Id: {F6723A6B-C651-4A72-9B28-9AA674B1CC35-at-aurion.nl} 10, 21 -- References: {201202031907.q13J7Q5T006851-at-ns.microscopy.com} 10, 21 -- To: marie.cantino-at-uconn.edu 10, 21 -- X-Mailer: Apple Mail (2.1084) 10, 21 -- Content-Transfer-Encoding: 8bit 10, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q16MP3HZ029504 ==============================End of - Headers==============================
I was also amused by the "driving test" analogy and really smiling at Steve Chapman's response.
I thought the old, Siemens Elmiskop had an great approach to an operational mistake: a red bar would loudly clunk down into place, in essence, screaming at the operator, "You made a BIG mistake, here! Call someone who knows what they are doing." I know someone who nearly fainted when this occurred (in the wee hours of the morning with no one else in the building). I never had the chance to use the instrument, though I really admired the high resolution images from this excellent instrument.
--
John J. Bozzola, Ph.D. Gleefully Retired Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University Carbondale, ILÂ 62901
On Mon, Feb 6, 2012 at 4:56 PM, {protrain-at-emcourses.com} wrote: } Hi All } } I am always amused when people talk about giving users a driving test before allowing them to use a microscope. Not only do I teach people to use electron microscopes I teach people to drive racing vehicles and it is only then that, with a smile, you realise just how much a driving test on a microscope differs dramatically from any other type of "driving test"!  When you drive a car or any other vehicle and make an error you frighten yourself and perhaps worry that you will be hurt, and you do not make the same mistake again.  Contrast this when there is no pain from making an error on a microscope.  The best computing system that I have seen that trained people correctly was MS Dos and MS Word One, here make any error and you were faced with a screen stating "fatal error", the machine would then crash and all would be lost.  You learned pretty quickly or just gave up! } } Yes people should undergo a microscope "driving test".  The test should be designed to produce a result that clearly demonstrates that the operator took into account every pertinent feature of the instrument.  From my experience a number of specimens are required each demanding an understanding of different features within the instrument.  I am often forced to say, when evaluating laboratories and their staff, you may have 1nm instruments but do you realise you have 5nm staff?  Staff testing in my mind is required and through repeated testing and under pressure the staff will develop their skills! } } Think of "Quality" the Japanese way - repeated evaluation and improvement. } } Some ramblings of an electron microscopist clocking up 48 years in the business. } } Steve } } Steve Chapman FRMS } Senior Consultant Protrain } For consultancy and training in electron microscopy world wide } Tel +44 (0)1280 816512  Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 } www.emcourses.com } } -----Original Message----- } From: M. Redigolo [mailto:marcela.redigolo-at-mail.wvu.edu] } Sent: 06 February 2012 17:53 } To: Allan Mitchell; Andrew D. Hollingsworth; Andrew Sullivan; Hands-Portman Ian; j.janssen-at-nki.nl; John Bozzola; Moore Jayma; Naomi McCallum; Patricia Nelson; protrain-at-emcourses.com; Richard E. Edelmann; sallystowe-at-gmail.com; Sherman Debra; Straszheim Warren E [BIOTC]; tommibarhorst-at-gmail.com; vray-at-partbeamsystech.com; Wim Hagen; Wim Van Den Broeck } Subject: New management topic - discussion } } Dear all: } } During the benchmarking this past week, two topics came up that it seems } many want to discuss. } So, I'm putting the topics out there to know what is the common practice in } other facilities. } } a) How to evaluate the efficiency (in terms of results, quality of data, } budget, etc) of management, fees and users? } b) Service contracts - self-service / all-inclusive / OEM service contract / } labor-only OEM service contract / third-party } service contract / per-hour paid service from OEM or third-party / } self-service with OEM support or with third-party support? } } Any inputs on those topics? } } Currently here, we have full service contract (all-inclusive) for both JEOL } instruments. In the case of the SEM, 5 years (3 years to go) } and for the TEM, we pay it yearly. This is one point that I might review and } change if I know that other model can also work fine and } save us money. } } We do collect a copy of every publication resulted from using the facilities }  Users are very good in sending us these copies since we use } them to justify increase of budget. Also, every user is requested to add an } acknowledgement line mentioning the facilities, in every publication. } Besides that, fees are reviewed yearly as I commented in the previous email. } In terms of usage of the instruments, in some cases (as it is for the TEM) } users undergo a "driver's test" before becoming unassisted users. } } OK. I promise I'll stop here and not crowd your inbox with long emails... } } Thanks again! } } Marcela. } } }       } ------ } Dr. Marcela Redigolo } Electron Microscopy Facility } WVU Shared Research Facilities } (304) 680-3007 } marcela.redigolo-at-mail.wvu.edu } } } }
==============================Original Headers============================== 6, 21 -- From bozzola-at-siu.edu Mon Feb 6 17:15:36 2012 6, 21 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16NFaRm014711 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 17:15:36 -0600 6, 21 -- Received: by vcbf13 with SMTP id f13so5147254vcb.0 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 06 Feb 2012 15:15:36 -0800 (PST) 6, 21 -- MIME-Version: 1.0 6, 21 -- Received: by 10.52.67.229 with SMTP id q5mr9587275vdt.14.1328570135043; Mon, 6, 21 -- 06 Feb 2012 15:15:35 -0800 (PST) 6, 21 -- Received: by 10.52.188.200 with HTTP; Mon, 6 Feb 2012 15:15:35 -0800 (PST) 6, 21 -- In-Reply-To: {002d01cce522$80b38840$821a98c0$-at-com} 6, 21 -- References: {4F30137A.00009F.02700-at-CRRHLSGHJLRM1} 6, 21 -- {002d01cce522$80b38840$821a98c0$-at-com} 6, 21 -- Date: Mon, 6 Feb 2012 17:15:35 -0600 6, 21 -- Message-ID: {CAKeCJ9w1dE0s+0-pu3GjsuCaFADcFCHx9+6uVY0iOrrSkRSzRQ-at-mail.gmail.com} 6, 21 -- Subject: Re: New management topic - discussion 6, 21 -- From: John Bozzola {bozzola-at-siu.edu} 6, 21 -- To: MSAListserver {Microscopy-at-microscopy.com} 6, 21 -- Content-Type: text/plain; charset=UTF-8 6, 21 -- Content-Transfer-Encoding: 8bit 6, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q16NFaRm014711 ==============================End of - Headers==============================
The Texas Society for Microscopy invites you to participate in its 2012 meeting taking place on the TCU campus Apr. 12-14. The meeting location is the Brown-Lupton University Union while the host hotel will be the SpringHill Suites by Marriott University Fort Worth. Shuttle service from the hotel to campus will be available. The abstract deadline is March 1. Additional meeting information and forms can be found on our website: http://www.texasmicroscopy.org.
Workshops scheduled for Thursday, April 12th, 1 – 4 PM Care & Feeding of Your Light Microscope and Light Source Sample Preparation Techniques for Materials Science – Choosing the Right Method by Robert Ranner, Product Manager, Leica Applications Specialist Etching, ion beam milling, slope cutting – what’s the difference? Target surface preparation – getting it down to size. Ultrasectioning – why go cryo? Hands-on demonstration: TXP – multi-functional target preparation device which can saw, mill, grind and polish TIC3X – automated ion beam milling system UC7/FC7 – advanced room and cryo temperature ultramicrotome
Invited Speakers Friday, April 13, 2012 Dr. Ray H. Baughman, Alan G. MacDiarmid NanoTech Institute, The University of Texas at Dallas - Biscrolling Nanofiber Sheets and Functional Guests into Multifunctional Yarns for Energy Applications
Dr. Aydogan Ozcan, Bio- and Nano-Photonics Laboratory, UCLA – Photonics-based Telemedicine Technologies Toward Smart Global Health Systems
==============================Original Headers============================== 4, 27 -- From r-holdford-at-ti.com Mon Feb 6 19:05:39 2012 4, 27 -- Received: from arroyo.ext.ti.com (arroyo.ext.ti.com [192.94.94.40]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q1715dGo000611 4, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 19:05:39 -0600 4, 27 -- Received: from dlep26.itg.ti.com ([157.170.170.121]) 4, 27 -- by arroyo.ext.ti.com (8.13.7/8.13.7) with ESMTP id q1715dGH031457 4, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 19:05:39 -0600 4, 27 -- Received: from DFLE70.ent.ti.com (localhost [127.0.0.1]) 4, 27 -- by dlep26.itg.ti.com (8.13.8/8.13.8) with ESMTP id q1715dtg026281 4, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 19:05:39 -0600 (CST) 4, 27 -- Received: from dlelxv22.itg.ti.com (172.17.1.197) by dfle70.ent.ti.com 4, 27 -- (128.247.5.40) with Microsoft SMTP Server id 14.1.323.3; Mon, 6 Feb 2012 4, 27 -- 19:05:38 -0600 4, 27 -- Received: from [128.247.8.175] (dta0142618.am.dhcp.ti.com [128.247.8.175]) by 4, 27 -- dlelxv22.itg.ti.com (8.13.8/8.13.8) with ESMTP id q1715cI3020448 for 4, 27 -- {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 19:05:38 -0600 4, 27 -- Message-ID: {4F3078E2.3050207-at-ti.com} 4, 27 -- Date: Mon, 6 Feb 2012 19:05:38 -0600 4, 27 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 27 -- Organization: SC Packaging MPI Team 4, 27 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:9.0) Gecko/20111222 Thunderbird/9.0.1 4, 27 -- MIME-Version: 1.0 4, 27 -- To: MSA Listserver {Microscopy-at-microscopy.com} 4, 27 -- Subject: Texas Society for Microscopy Spring Meeting Apr. 12-14 at TCU in 4, 27 -- Ft. Worth, TX 4, 27 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 4, 27 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Ah, fond memories. My first real experience in TEM (besides playing with a Philips 75 as an undergrad) was in the lab of Humberto Fernandez-Moran at the University of Chicago. We had Siemens Elmiskop 1 and 1a microscopes. Oh yes...saw that red bar on numerous occasions. The scope had a mechanical valve block and if it was not turned properly the bar sprang up. Made enough noise so those in the next room knew that you did a no-no.
However, I still have glass negatives of some images taken from that instrument. I have never seen better coming from any modern W-filament instrument. Check out the T4 phage (at www.dsimagingllc.com under "Images } animal"), which was taken 45 years ago when I had been using the Siemens Elmiskop I for about 6 months. Before being able to look at real sample, we had to produce a perfect objective stigmation series on a hole sample at very high mag, using the mechanical stigmator system available in those days. I really learned how to stigmate and focus incredibly well with almost no illumination. It has been a skill that I still value extremely highly. Spent a lot of time in the dark working on that microscope along with a young grad student who became my husband. There is a lot to be said for spending time in the dark! One big advantage we had was using what I believe were the first pointed filaments that were made in the lab. But that is another story. For those calculating my age, I was very young at the time to have access to such an instrument.....
Debby Sherman
On 2/6/12 6:16 PM, "John Bozzola, Phd. Director" {bozzola-at-siu.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was also amused by the "driving test" analogy and really smiling at } Steve Chapman's response. } } I thought the old, Siemens Elmiskop had an great approach to an } operational mistake: a red bar would loudly clunk down into place, in } essence, screaming at the operator, "You made a BIG mistake, here! } Call someone who knows what they are doing." I know someone who nearly } fainted when this occurred (in the wee hours of the morning with no } one else in the building). I never had the chance to use the } instrument, though I really admired the high resolution images from } this excellent instrument.
==============================Original Headers============================== 9, 31 -- From dsherman-at-purdue.edu Mon Feb 6 19:46:52 2012 9, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q171kqvY018049 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 19:46:52 -0600 9, 31 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 9, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id q171kpi1012216 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 20:46:52 -0500 9, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.60]) by WPPEXHUB04H.purdue.lcl 9, 31 -- ([::1]) with mapi; Mon, 6 Feb 2012 20:46:51 -0500 9, 31 -- From: "Sherman, Debra" {dsherman-at-purdue.edu} 9, 31 -- To: "John Bozzola, Phd. Director" {bozzola-at-siu.edu} , 9, 31 -- "message to: MSA list" 9, 31 -- {microscopy-at-microscopy.com} 9, 31 -- Date: Mon, 6 Feb 2012 20:46:49 -0500 9, 31 -- Subject: Re: [Microscopy] Re: New management topic - discussion 9, 31 -- Thread-Topic: [Microscopy] Re: New management topic - discussion 9, 31 -- Thread-Index: AczlJWs3oKk+PgfNRzqsvs6hY3ZtTgAFPAfJ 9, 31 -- Message-ID: {CB55ECB9.1599A%dsherman-at-purdue.edu} 9, 31 -- In-Reply-To: {201202062316.q16NGt8u017334-at-ns.microscopy.com} 9, 31 -- Accept-Language: en-US 9, 31 -- Content-Language: en-US 9, 31 -- X-MS-Has-Attach: 9, 31 -- X-MS-TNEF-Correlator: 9, 31 -- user-agent: Microsoft-Entourage/13.11.0.110726 9, 31 -- acceptlanguage: en-US 9, 31 -- Content-Type: text/plain; charset="us-ascii" 9, 31 -- MIME-Version: 1.0 9, 31 -- X-PMX-Version: 5.5.9.388399 9, 31 -- X-PerlMx-Virus-Scanned: Yes 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q171kqvY018049 ==============================End of - Headers==============================
I imagine you would have difficulty using AFM if you needed to count things attached to particles. AFM gives you a 2D projection of the sample, you would miss anything under or "shadowed" by the particle or anything else. AFM doesn't do reentrant profiles (typically). If all you need is to see if something's attached, get some size information (caution about reentrant profiles again), and you have well characterized particles to attach to, AFM might be able to help you.
Jonathan Abbott
On Feb 6, 2012, at 7:48, marie.cantino-at-uconn.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Stefan, } } I do understand the difference between mass and length. } } The problem is that the clients usually can only give us the molecular } weight and (if we're lucky) the number of molecules per particle. If } they knew how the proteins/peptides were arranged or the linear } dimensions of the coating they wouldn't need to come to me. What I'm } trying to find out from the listserver or the literature is whether we } can reasonably expect to be able to detect a medium sized protein such } as IgG on an electron dense gold particle, given the various artifacts } of techniques such as negative staining. } } We only have TEM (w/a tungsten emitter) and FESEM in our facility, but } I can send them elsewhere for AFM. } } Marie } } On Feb 6, 2012, at 5:33 AM, nizets2-at-yahoo.com wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi Marie, } } } } I like these projects which put the techniques to their limits. } } I think the reason why you have difficulties finding publications on } } this matter and that the question is not trivial at all. } } First of all, and to start with a relaxed note, the mixing of kDa } } und nm in one sentence made me smile because it makes as much sense } } as asking if 1kg of something fits in one meter. } } The Dalton is a unit of mass and does not give a good indication of } } the dimension or the shape of a molecule. } } That said, I seem to remember from the past answers of our emminent } } immunoglobulinologist Jan Leunissen that the size of an IgG is } } several nm, I think more than 5nm. } } Which means, in your case, that you should not expect more that a } } few molecules per nanoparticle, except if they form multilayers. } } As for the techniques, I wonder if a SEM could resolve this } } structure. Although I have experience with analytical low res SEMs, } } I don't know the limits of modern FEG SEMs. } } I would be happy if some listers would care to share their opinion } } on this matter. } } It seems that AFM (atomic force microscopy) would be able to resolve } } this structure, but I wonder if it would be possible in this } } condition because AFM works on flat substrate and here you have } } round particles. } } It would probably be possible to find some answers using cry- } } microscopy, the same way scientists use it to resolve viral } } structures at several anstrom resolution. It probably depends on how } } much time and money your clients are wanting to spend and how } } critical this information is for them. } } } } Regards, } } Stephane } } } } ----- Original Message ----- } } X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} } } To: nizets2-at-yahoo.com } } Cc: } } Sent: Friday, February 3, 2012 8:10 PM } } Subject: [Microscopy] TEM of functionalized nanoparticles } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } In recent years we have been getting requests to image functionalized } } nanoparticles, such as 10 nm gold with proteins or peptides attached. } } Generally the clients want to know how thick or large the organic } } "shell" is, or how the protein/peptide is arranged on the surface. } } Most of our experience has been with either inorganic particles (which } } we view without staining) or larger organic structures such as viral } } procapsids or large proteins (e.g., myosin) or protein assemblies, } } which we negative stain. My questions for this list are as follows: } } } } - have others on the list had good luck imaging these kinds of } } conjugates at the EM level, and if so, using what techniques? } } - how much organic material must one have associated with the } } nanoparticle to see it by negative staining or other methods? For } } example, how hard is it to detect a 150 kD protein on a 10 nm gold } } particle? An example would be IgG attached to a 10 nm gold particle. } } - can you suggest any publications or review articles on the subject? } } For some reason I'm not having much luck with my Pubmed search } } strategies. A recent paper by Cao and Mao seems to deal mainly with } } small gold particles on much larger protein aggregates, but perhaps } } there are others out there. } } } } Many thanks (in advance) for your suggestions. } } } } Dr. Marie E. Cantino } } Associate Professor of Physiology and Neurobiology } } Director, Electron Microscopy Laboratory } } University of Connecticut, Unit 3242 } } Phone: 860-486-3588 } } Fax: 860-486-6369 } } } } } } ==============================Original } } Headers============================== } } 5, 18 -- From marie.cantino-at-uconn.edu Fri Feb 3 13:07:15 2012 } } 5, 18 -- Received: from mta1.uits.uconn.edu (mailscanner.uconn.edu } } [137.99.25.234]) } } 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id q13J7FK2006567 } } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 } } 13:07:15 -0600 } } 5, 18 -- Received: from d47h9.public.uconn.edu } } (d47h9.public.uconn.edu [137.99.47.9]) } } 5, 18 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 4D02634B9A } } 5, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Feb 2012 } } 14:07:15 -0500 (EST) } } 5, 18 -- Message-Id: {6803418B-8131-4A98-990F-158505836E6E-at-uconn.edu} } } 5, 18 -- From: Marie Cantino {marie.cantino-at-uconn.edu} } } 5, 18 -- To: Microscopy-at-Microscopy.Com } } 5, 18 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } } delsp=yes } } 5, 18 -- Content-Transfer-Encoding: 7bit } } 5, 18 -- Mime-Version: 1.0 (Apple Message framework v936) } } 5, 18 -- Subject: TEM of functionalized nanoparticles } } 5, 18 -- Date: Fri, 3 Feb 2012 14:07:15 -0500 } } 5, 18 -- X-Mailer: Apple Mail (2.936) } } 5, 18 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 } } 5, 18 -- X-Virus-Status: Clean } } ==============================End of - } } Headers============================== } } } } } } } } ==============================Original } } Headers============================== } } 15, 42 -- From nizets2-at-yahoo.com Mon Feb 6 04:28:31 2012 } } 15, 42 -- Received: from nm9.bullet.mail.sp2.yahoo.com } } (nm9.bullet.mail.sp2.yahoo.com [98.139.91.79]) } } 15, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } id q16ASUSx003271 } } 15, 42 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 04:28:31 } } -0600 } } 15, 42 -- Received: from [98.139.91.62] by } } nm9.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 } } 15, 42 -- Received: from [98.139.91.23] by } } tm2.bullet.mail.sp2.yahoo.com with NNFMP; 06 Feb 2012 10:28:30 -0000 } } 15, 42 -- Received: from [127.0.0.1] by omp1023.mail.sp2.yahoo.com } } with NNFMP; 06 Feb 2012 10:28:30 -0000 } } 15, 42 -- X-Yahoo-Newman-Property: ymail-3 } } 15, 42 -- X-Yahoo-Newman-Id: } } 481221.57336.bm-at-omp1023.mail.sp2.yahoo.com } } 15, 42 -- Received: (qmail 64306 invoked by uid 60001); 6 Feb 2012 } } 10:28:30 -0000 } } 15, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } d=yahoo.com; s=s1024; t=1328524109; bh= } } +xZoCP0S7BlCXixrJBv6fCBgmv9JLm9A9772ftf9y9w=; h=X-YMail- } } OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply- } } To:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content- } } Transfer-Encoding; } } b } } = } } DhiSJ4sQkcV0vFmz7NomZjwgCjpBj4FAdn7ZD2wksosyYhu8N5W2UqbTQyeXCJK4oVawc69mSzTPOiPLJMDjlVLrxuWj } } +2HE7+negf7O2gV72PfH1xIgxl0oKW5yjsdKajsyvKAsKy1AyxjksZ4FKsRyicVW6P } } +XS3bODk3UHBI= } } 15, 42 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } } 15, 42 -- s=s1024; d=yahoo.com; } } 15, 42 -- h=X-YMail-OSG:Received:X-Mailer:References:Message- } } ID:Date:From:Reply-To:Subject:To:Cc:In-Reply-To:MIME-Version:Content- } } Type:Content-Transfer-Encoding; } } 15, 42 -- b=0z5d16psGKpudCX/OEBR6HSJZodETUvunNzVkE2Bdt/ } } 50FwYt985UVUxt } } + } } wGQv0dJpC6PSS5C1UVuucgfGoIke8emqTSu5ngf9h5IlUbvg19SDLYgnCq56YYozOelWUhS7ZeiXK6XJoArVPn97DAVKnn36IdAtBG77TEHRcq0XE } } =; } } 15, 42 -- X-YMail-OSG: mU9.CMYVM1nU5RWQzCOCo2tCXdcc5rBWDHvkrMrE8dmtNg2 } } 15, 42 -- } } ghcyqmxIav.mWZe55tGfPx8hB73Ku2Go_g2YcxIYVxYu.xIQcjRB8dOscnIl } } 15, 42 -- ItGCm03ET3Xk9rdFmB5AyEOTu. } } 5q67LQ3BVRuBgGI4Dyb1JBuFnhh6LX33Ge } } 15, 42 -- } } eZCSbcxsrxDfym2SvNF9HjWSjNSQhA3pmSisSwcORyEwAK7pVR6xPyjASZja } } 15, 42 -- } } 81UDvA.AJMLbz_lavTzjKSa7tsBX1HKarcZ_GAqFWj8E8vgim8xl_2cb9ajC } } 15, 42 -- } } J6x_Et5Oxs8J8PMTbE6fQbAsSz1rSnFYUn_0t2yB5pB4NvXbsBmtxHUE9IAq } } 15, 42 -- } } cGxGthxOtfOCAVTV5sIWmT0..AZylbKQ7UAE45AJjl9D4Pl9Kheo._ZrOqI7 } } 15, 42 -- } } 6JOY6X1VgqTVqTbHUdl2hNXyBKit3PZ5.7H4NFTszDoQ1wMaPo7PGZ7MJj6T } } 15, 42 -- } } ox4fhW2_ISZ4ykqYrKGhEE3K0h0_x9_whxj_V4afxT8ssY5stADMbOyv75Hf } } 15, 42 -- } } IV65YjtPjKc0RCBkjCk.JYib0q649_W.AcC2G7R7T0R_obP_lnUnSx67zPct } } 15, 42 -- uAa96Geh729v3Ci.cpDJf6V93rfKrN65tq6cZtHoQ5c95q36LP3UHZ.u. } } 7No } } 15, 42 -- - } } 15, 42 -- Received: from [80.122.101.100] by } } web110815.mail.gq1.yahoo.com via HTTP; Mon, 06 Feb 2012 02:28:29 PST } } 15, 42 -- X-Mailer: YahooMailWebService/0.8.116.338427 } } 15, 42 -- References: {201202031910.q13JASuh012105-at-ns.microscopy.com} } } 15, 42 -- Message-ID: {1328524109.50274.YahooMailNeo-at-web110815.mail.gq1.yahoo.com } } } } } 15, 42 -- Date: Mon, 6 Feb 2012 02:28:29 -0800 (PST) } } 15, 42 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } 15, 42 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } } 15, 42 -- Subject: Re: [Microscopy] TEM of functionalized } } nanoparticles } } 15, 42 -- To: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} } } 15, 42 -- Cc: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } } 15, 42 -- In-Reply-To: {201202031910.q13JASuh012105-at-ns.microscopy.com} } } 15, 42 -- MIME-Version: 1.0 } } 15, 42 -- Content-Type: text/plain; charset=iso-8859-1 } } 15, 42 -- Content-Transfer-Encoding: 8bit } } 15, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id q16ASUSx003271 } } ==============================End of - } } Headers============================== } } Dr. Marie E. Cantino } Associate Professor of Physiology and Neurobiology } Director, Electron Microscopy Laboratory } University of Connecticut, Unit 3242 } Phone: 860-486-3588 } Fax: 860-486-6369 } } } ==============================Original Headers============================== } 9, 20 -- From marie.cantino-at-uconn.edu Mon Feb 6 08:40:11 2012 } 9, 20 -- Received: from mta1.uits.uconn.edu (smtp.uconn.edu [137.99.25.234]) } 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16EeB9q015449 } 9, 20 -- for {microscopy-at-Microscopy.Com} ; Mon, 6 Feb 2012 08:40:11 -0600 } 9, 20 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9]) } 9, 20 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id E24A83499C } 9, 20 -- for {microscopy-at-Microscopy.Com} ; Mon, 6 Feb 2012 09:40:10 -0500 (EST) } 9, 20 -- Message-Id: {E71EBD9F-CDF7-4B2B-A401-0FFA4339EE6D-at-uconn.edu} } 9, 20 -- From: Marie Cantino {marie.cantino-at-uconn.edu} } 9, 20 -- To: microscopy-at-Microscopy.Com } 9, 20 -- In-Reply-To: {201202061033.q16AXXWw011504-at-ns.microscopy.com} } 9, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 9, 20 -- Content-Transfer-Encoding: 7bit } 9, 20 -- Mime-Version: 1.0 (Apple Message framework v936) } 9, 20 -- Subject: Re: [Microscopy] Re: TEM of functionalized nanoparticles } 9, 20 -- Date: Mon, 6 Feb 2012 09:40:10 -0500 } 9, 20 -- References: {201202061033.q16AXXWw011504-at-ns.microscopy.com} } 9, 20 -- X-Mailer: Apple Mail (2.936) } 9, 20 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 } 9, 20 -- X-Virus-Status: Clean } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 34 -- From forzaabbott-at-gmail.com Mon Feb 6 23:43:07 2012 5, 34 -- Received: from mail-pw0-f41.google.com (mail-pw0-f41.google.com [209.85.160.41]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q175h68C005803 5, 34 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 23:43:07 -0600 5, 34 -- Received: by pbaa12 with SMTP id a12so6215469pba.0 5, 34 -- for {microscopy-at-microscopy.com} ; Mon, 06 Feb 2012 21:43:06 -0800 (PST) 5, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 34 -- d=gmail.com; s=gamma; 5, 34 -- h=references:in-reply-to:mime-version:content-transfer-encoding 5, 34 -- :content-type:message-id:cc:x-mailer:from:subject:date:to; 5, 34 -- bh=DWWBMl9bmdwYv5Iq62vUcy6Ja+jDgRyyEecWMUiyCg8=; 5, 34 -- b=YnHQzMcR26N/KX0ZaVSmFt3TfyKquN9Oap6UorX8VT/OJlvui6FOROCvSVIilW1sGU 5, 34 -- dZLnkDpvXWNYg2kFTFwaC4AhToCzu2QCzgilIhd7HqjUUYYE1e6rqjBNHOVT30o5vvG7 5, 34 -- bJjFxcVCZGjKkqbm9YfEN3RSri1Oi3Z8+fLj8= 5, 34 -- Received: by 10.68.233.74 with SMTP id tu10mr30125209pbc.98.1328593385528; 5, 34 -- Mon, 06 Feb 2012 21:43:05 -0800 (PST) 5, 34 -- Received: from [192.168.1.102] ([76.8.203.252]) 5, 34 -- by mx.google.com with ESMTPS id x4sm44015268pbx.16.2012.02.06.21.43.01 5, 34 -- (version=TLSv1/SSLv3 cipher=OTHER); 5, 34 -- Mon, 06 Feb 2012 21:43:03 -0800 (PST) 5, 34 -- References: {201202061448.q16Emvak008158-at-ns.microscopy.com} 5, 34 -- In-Reply-To: {201202061448.q16Emvak008158-at-ns.microscopy.com} 5, 34 -- Mime-Version: 1.0 (1.0) 5, 34 -- Content-Type: text/plain; 5, 34 -- charset=us-ascii 5, 34 -- Message-Id: {399EB67D-26F4-43F3-A988-391BDED01321-at-gmail.com} 5, 34 -- Cc: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 34 -- X-Mailer: iPad Mail (9A405) 5, 34 -- From: Jonathan Abbott {forzaabbott-at-gmail.com} 5, 34 -- Subject: Re: [Microscopy] TEM of functionalized nanoparticles 5, 34 -- Date: Mon, 6 Feb 2012 22:43:00 -0700 5, 34 -- To: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu} 5, 34 -- Content-Transfer-Encoding: 8bit 5, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q175h68C005803 ==============================End of - Headers==============================
I had a session on a Philips CM20 today. I was working at relatively high mag and I wanted to shift the image instead of using the mechanical stage shifts. For the life of me, I could not figure out how to do it. I’m not that familiar with the instrument, but I’m sure that it can be done. Can anyone tell me the controls for it?
-Scott
Scott D. Walck, Ph.D. 5234 Sandalwood PL Oceanside, CA 92056
S.Walck-at-cox.net SWalck65-at-gmail.com
(760) 685-2815 (Cell) (760) 758-4384 (Home)
==============================Original Headers============================== 9, 36 -- From s.walck-at-cox.net Tue Feb 7 02:10:41 2012 9, 36 -- Received: from eastrmfepo201.cox.net (eastrmfepo201.cox.net [68.230.241.216]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q178AfkY024588 9, 36 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2012 02:10:41 -0600 9, 36 -- Received: from eastrmimpo109.cox.net ([68.230.241.222]) 9, 36 -- by eastrmfepo201.cox.net 9, 36 -- (InterMail vM.8.01.04.00 201-2260-137-20101110) with ESMTP 9, 36 -- id {20120207081031.HKN4752.eastrmfepo201.cox.net-at-eastrmimpo109.cox.net} 9, 36 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2012 03:10:31 -0500 9, 36 -- Received: from WalckPC ([72.197.11.236]) 9, 36 -- by eastrmimpo109.cox.net with bizsmtp 9, 36 -- id WwAV1i00G55aDzc02wAW7w; Tue, 07 Feb 2012 03:10:30 -0500 9, 36 -- X-CT-Class: Clean 9, 36 -- X-CT-Score: 0.00 9, 36 -- X-CT-RefID: str=0001.0A020201.4F30DC76.011C,ss=1,re=0.000,fgs=0 9, 36 -- X-CT-Spam: 0 9, 36 -- X-Authority-Analysis: v=1.1 cv=EYzcOfuzocFB4Uh5a8S+SAtfBMCIXs4UTLohtyR2Crs= 9, 36 -- c=1 sm=1 a=G8Uczd0VNMoA:10 a=8nJEP1OIZ-IA:10 a=s2s4rfcuBpqIM77q7Z6Q4A==:17 9, 36 -- a=kviXuzpPAAAA:8 a=pGLkceISAAAA:8 a=Ju9S2heQeK40OAsmjt4A:9 a=wPNLvfGTeEIA:10 9, 36 -- a=Q0FMIQXHCckA:10 a=bn5IZYmGmUcA:10 a=qvqOS3AttqwA:10 a=UfgbimgSl04A:10 9, 36 -- a=4vB-4DCPJfMA:10 a=MSl-tDqOz04A:10 a=s2s4rfcuBpqIM77q7Z6Q4A==:117 9, 36 -- X-CM-Score: 0.00 9, 36 -- Authentication-Results: cox.net; auth=pass (LOGIN) smtp.auth=S.Walck-at-cox.net 9, 36 -- From: "Scott Walck" {S.Walck-at-cox.net} 9, 36 -- To: {microscopy-at-microscopy.com} 9, 36 -- Subject: image shift on CM20 9, 36 -- Date: Tue, 7 Feb 2012 00:10:28 -0800 9, 36 -- Message-ID: {012401cce56f$f49619b0$ddc24d10$-at-cox.net} 9, 36 -- MIME-Version: 1.0 9, 36 -- Content-Type: text/plain; 9, 36 -- charset="iso-8859-1" 9, 36 -- X-Mailer: Microsoft Outlook 14.0 9, 36 -- Thread-Index: Aczlb7oHH/IOEJF7QsewWmDtysOk0g== 9, 36 -- Content-Language: en-us 9, 36 -- Content-Transfer-Encoding: 8bit 9, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q178AfkY024588 ==============================End of - Headers==============================
let me briefly comment on one sentence in Jonathan's comment from yesterday.
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
next microscopy conferences: http://www.emc2012.org.uk/ EMC 2012 in Manchester, UK http://www.mc2013.de/ MC2013 in Regensburg, Germany
} } } } I imagine you would have difficulty using AFM if you needed to count things } attached to particles. } AFM gives you a 2D projection of the sample,
no, this is not the case. AFM will give you an image representing the surface / surface relief of the sample, but not a 2D projection (in the sense as the TEM does). You will not get any information of the internal parts of the sample.
} you would miss anything under or "shadowed" by the particle or anything else. AFM } doesn't do reentrant profiles (typically).
} If all you need is to see if something's attached, get some size information } (caution about reentrant profiles again), and you have well characterized } particles to attach to, AFM might be able to help you.
here, I fully agree. Kind regards, Reinhard
==============================Original Headers============================== 11, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Tue Feb 7 03:10:45 2012 11, 25 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q179AeBI009871 11, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Feb 2012 03:10:44 -0600 11, 25 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 11, 25 -- by localhost (Postfix) with SMTP id 90EFB3A9D 11, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Feb 2012 10:10:38 +0100 (CET) 11, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 11, 25 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id 6804F1EB2 11, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Feb 2012 10:10:38 +0100 (CET) 11, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 11, 25 -- with Novell_GroupWise; Tue, 07 Feb 2012 10:10:39 +0100 11, 25 -- Message-Id: {4F30F89D02000054000291B4-at-gwsmtp1.uni-regensburg.de} 11, 25 -- X-Mailer: Novell GroupWise Internet Agent 12.0.0 11, 25 -- Date: Tue, 07 Feb 2012 10:10:37 +0100 11, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 11, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 11, 25 -- Subject: TEM / AFM and nanoparticles 11, 25 -- References: {201202070543.q175hNB3006115-at-ns.microscopy.com} 11, 25 -- In-Reply-To: {201202070543.q175hNB3006115-at-ns.microscopy.com} 11, 25 -- Mime-Version: 1.0 11, 25 -- Content-Type: text/plain; charset=US-ASCII 11, 25 -- Content-Disposition: inline 11, 25 -- Content-Transfer-Encoding: 8bit 11, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q179AeBI009871 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both amit.welcomes.u-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: amit.welcomes.u-at-gmail.com Name: Amit
Organization: Indian institute of science
Title-Subject: [Filtered] Diagonal bands in STEM mode
Message: we recently installed JEOL JEM-2100F microscope. rest is working fine but in STEM mode there are Diagonal Bands of alternate brightness coming(all bands or strips are of same thickness, brightness and contrast). on increasing scan time band's thickness first increases then at even higher scan times it decreases. Engineers are on it but cant pin point the fault. i was wondering if anyone encountered it before or knows why this is happening
"stripes" in images are often the result of interference. It often helps to determine the frequency to eliminate sources (for example if the frequency is 50 or 60 Hz, it usually comes from a power source). Have you tried to determine the frequency?
Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, February 07, 2012 3:40 AM To: mike.bode-at-resaltatech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both amit.welcomes.u-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: amit.welcomes.u-at-gmail.com Name: Amit
Organization: Indian institute of science
Title-Subject: [Filtered] Diagonal bands in STEM mode
Message: we recently installed JEOL JEM-2100F microscope. rest is working fine but in STEM mode there are Diagonal Bands of alternate brightness coming(all bands or strips are of same thickness, brightness and contrast). on increasing scan time band's thickness first increases then at even higher scan times it decreases. Engineers are on it but cant pin point the fault. i was wondering if anyone encountered it before or knows why this is happening
Regarding AFM of gold nanoparticles with proteins attached, I have some experience with this.
As was mentioned, AFM (high resolution at least) works best with flat samples. You can certainly images round particles with no difficulty, but you will not normally get many details. What you will see is a “blob”, most likely. Now, if your particles are nice (i.e. close to monodisperse), and your protein size is significant compared to your gold particle size, what you can do, is compare the “unfunctionalised” size (height of the blob) to the functionalised size, and use this difference as a measure of how much, if any, protein got attached. AFM has very good resolution in height, getting images good enough to make these measurements would be simple. The limiting factor here will most likely be, what is the significance of your height measurements, given the range of height of the “bare” particles. Also, how many AFM images you can bear to measure, to get a significant sample.
By the way, no, we don’t directly compare kDa and nanometres, but I understood exactly why the poster said this...ask a biochemist how big their protein is, and they will always reply in terms of Daltons. We can, however make a *guess* at the size based on the height, and I can tell you that your 150kDa particle WILL make a significant difference to the 10 nm AuNP´s diameter, since I have done this with much smaller proteins.
On the other hand, there is no doubt that if the negative staining works well, and you can image both the the AuNPs and proteins, it will be a nicer result, and probably less time consuming.
Good Luck!
Pete.
==============================Original Headers============================== 11, 20 -- From petereaton-at-hotmail.com Tue Feb 7 11:30:56 2012 11, 20 -- Received: from snt0-omc3-s23.snt0.hotmail.com (snt0-omc3-s23.snt0.hotmail.com [65.55.90.162]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q17HUuKI005821 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2012 11:30:56 -0600 11, 20 -- Received: from SNT139-W62 ([65.55.90.136]) by snt0-omc3-s23.snt0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 11, 20 -- Tue, 7 Feb 2012 09:30:56 -0800 11, 20 -- Message-ID: {SNT139-W62F773C3410166EDB29535C8750-at-phx.gbl} 11, 20 -- X-Originating-IP: [193.136.24.67] 11, 20 -- From: Peter Eaton {petereaton-at-hotmail.com} 11, 20 -- To: {microscopy-at-microscopy.com} 11, 20 -- Subject: TEM/AFM and nanoparticles 11, 20 -- Date: Tue, 7 Feb 2012 17:30:55 +0000 11, 20 -- Importance: Normal 11, 20 -- In-Reply-To: {SNT139-W67102750CFDD569369865C8750-at-phx.gbl} 11, 20 -- References: {SNT139-W67102750CFDD569369865C8750-at-phx.gbl} 11, 20 -- Content-Type: text/plain; charset="Windows-1252" 11, 20 -- MIME-Version: 1.0 11, 20 -- X-OriginalArrivalTime: 07 Feb 2012 17:30:56.0268 (UTC) FILETIME=[3FBB2CC0:01CCE5BE] 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q17HUuKI005821 ==============================End of - Headers==============================
Thank you, Wim. I believe this is what I want. I didn't use the HR-TEM mode, I used the TEM mode. Wim Hagen's direct email message to me included a readout image of the HR-TEM page and it shows the Image Shift as well as some other HR-TEM functions. His answer was to use the HR-TEM mode, choose Parameter page and the appropriate control pages come up.
Chad Parish, John Minter, and Reinhard Rachel suggested using the Alignment-Image Shift. I did try that and all it did when I touched the multi-knobs was to beep at me. John said that this function is for keeping the different mags aligned.
Thank you all for your help. Next time I'm on the microscope, I'll try it.
-Scott
Scott D. Walck, Ph.D. 5234 Sandalwood PL Oceanside, CA 92056
S.Walck-at-cox.net SWalck65-at-gmail.com
(760) 685-2815 (Cell) (760) 758-4384 (Home)
-----Original Message----- X-from: Wim Hagen [mailto:wim.hagen-at-me.com] Sent: Tuesday, February 07, 2012 12:28 AM To: S.Walck-at-cox.net
Another thing you could try is high-angle Pt/C shadowing.
I don't know if it's easier to find an AFM or a working high vacuum evaporator these days, though!
Andy Bowling
==============================Original Headers============================== 6, 38 -- From AJBowling-at-dow.com Tue Feb 7 14:57:10 2012 6, 38 -- Received: from mail85.messagelabs.com (mail85.messagelabs.com [216.82.241.211]) 6, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q17Kv9Gr011308 6, 38 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2012 14:57:09 -0600 6, 38 -- X-Env-Sender: AJBowling-at-dow.com 6, 38 -- X-Msg-Ref: server-9.tower-85.messagelabs.com!1328648227!8686331!2 6, 38 -- X-Originating-IP: [204.136.184.21] 6, 38 -- X-StarScan-Version: 6.5.5; banners=-,-,- 6, 38 -- X-VirusChecked: Checked 6, 38 -- Received: (qmail 20908 invoked from network); 7 Feb 2012 20:57:09 -0000 6, 38 -- Received: from mail3.dow.com (HELO USFRPMDOWS001.dow.com) (204.136.184.21) 6, 38 -- by server-9.tower-85.messagelabs.com with RC4-SHA encrypted SMTP; 7 Feb 2012 20:57:09 -0000 6, 38 -- Received: from USMDLMDOWC004.dow.com ([163.198.211.232]) by USFRPMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.4675); 6, 38 -- Tue, 7 Feb 2012 14:57:00 -0600 6, 38 -- Received: from mail.bsnconnect.com ([165.216.20.17]) by USMDLMDOWC004.dow.com with Microsoft SMTPSVC(6.0.3790.4675); 6, 38 -- Tue, 7 Feb 2012 15:56:48 -0500 6, 38 -- Received: from 046-CH1MPN1-043.046d.mgd.msft.net ([169.254.3.118]) by 6, 38 -- 046-CH1MMR1-005.046d.mgd.msft.net ([165.216.20.17]) with mapi id 6, 38 -- 14.01.0355.003; Tue, 7 Feb 2012 14:56:37 -0600 6, 38 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com} 6, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 38 -- Subject: RE: [Microscopy] TEM/AFM and nanoparticles 6, 38 -- Thread-Topic: [Microscopy] TEM/AFM and nanoparticles 6, 38 -- Thread-Index: AQHM5b83UA0Qoocma0GK3QPWMKiAkZYx5zMA 6, 38 -- Date: Tue, 7 Feb 2012 20:56:38 +0000 6, 38 -- Message-ID: {62E8C73DFC76AB49A7317AE84720EB6405DD3D-at-046-CH1MPN1-043.046d.mgd.msft.net} 6, 38 -- References: {201202071737.q17HbGTU018713-at-ns.microscopy.com} 6, 38 -- In-Reply-To: {201202071737.q17HbGTU018713-at-ns.microscopy.com} 6, 38 -- Accept-Language: en-US 6, 38 -- Content-Language: en-US 6, 38 -- X-MS-Has-Attach: 6, 38 -- X-MS-TNEF-Correlator: 6, 38 -- x-originating-ip: [140.170.173.81] 6, 38 -- Content-Type: text/plain; charset="us-ascii" 6, 38 -- MIME-Version: 1.0 6, 38 -- X-OriginalArrivalTime: 07 Feb 2012 20:56:49.0135 (UTC) FILETIME=[029D17F0:01CCE5DB] 6, 38 -- Content-Transfer-Encoding: 8bit 6, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q17Kv9Gr011308 ==============================End of - Headers==============================
I already gave my opinion to Marie about this offline but since there were several answers in the list going in this direction I would like to share some thoughts with the list.
Negative staining may work. This means: you may see your proteins, you may get a result. You may even get a nice picture. But hey, are we artists or are we scientists? Our job is not to get nice pictures, it is to get meaningful pictures.
Marie wants to know how the antibodies (or other proteins) are organized around gold particles in a matrix, most probably water or aqueous solution. I am not sure the best way to know that is by drying the sample with a heavy stain. Do you really think it may not interfere with the binding forces between the antibody and the gold particle? I wouldn't bet a cent on it!
Regards, Stephane
----- Original Message ----- X-from: "petereaton-at-hotmail.com" {petereaton-at-hotmail.com} To: nizets2-at-yahoo.com Cc: Sent: Tuesday, February 7, 2012 6:33 PM
On the other hand, there is no doubt that if the negative staining works well, and you can image both the the AuNPs and proteins, it will be a nicer result, and probably less time consuming.
Good Luck!
Pete.
==============================Original Headers============================== 11, 20 -- From petereaton-at-hotmail.com Tue Feb 7 11:30:56 2012 11, 20 -- Received: from snt0-omc3-s23.snt0.hotmail.com (snt0-omc3-s23.snt0.hotmail.com [65.55.90.162]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q17HUuKI005821 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2012 11:30:56 -0600 11, 20 -- Received: from SNT139-W62 ([65.55.90.136]) by snt0-omc3-s23.snt0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 11, 20 -- Tue, 7 Feb 2012 09:30:56 -0800 11, 20 -- Message-ID: {SNT139-W62F773C3410166EDB29535C8750-at-phx.gbl} 11, 20 -- X-Originating-IP: [193.136.24.67] 11, 20 -- From: Peter Eaton {petereaton-at-hotmail.com} 11, 20 -- To: {microscopy-at-microscopy.com} 11, 20 -- Subject: TEM/AFM and nanoparticles 11, 20 -- Date: Tue, 7 Feb 2012 17:30:55 +0000 11, 20 -- Importance: Normal 11, 20 -- In-Reply-To: {SNT139-W67102750CFDD569369865C8750-at-phx.gbl} 11, 20 -- References: {SNT139-W67102750CFDD569369865C8750-at-phx.gbl} 11, 20 -- Content-Type: text/plain; charset="Windows-1252" 11, 20 -- MIME-Version: 1.0 11, 20 -- X-OriginalArrivalTime: 07 Feb 2012 17:30:56.0268 (UTC) FILETIME=[3FBB2CC0:01CCE5BE] 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q17HUuKI005821 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 40 -- From nizets2-at-yahoo.com Wed Feb 8 04:18:05 2012 18, 40 -- Received: from nm14-vm1.bullet.mail.ne1.yahoo.com (nm14-vm1.bullet.mail.ne1.yahoo.com [98.138.91.38]) 18, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q18AI4PX009989 18, 40 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 04:18:05 -0600 18, 40 -- Received: from [98.138.90.57] by nm14.bullet.mail.ne1.yahoo.com with NNFMP; 08 Feb 2012 10:18:04 -0000 18, 40 -- Received: from [98.138.89.244] by tm10.bullet.mail.ne1.yahoo.com with NNFMP; 08 Feb 2012 10:18:04 -0000 18, 40 -- Received: from [127.0.0.1] by omp1058.mail.ne1.yahoo.com with NNFMP; 08 Feb 2012 10:18:04 -0000 18, 40 -- X-Yahoo-Newman-Property: ymail-3 18, 40 -- X-Yahoo-Newman-Id: 260981.33175.bm-at-omp1058.mail.ne1.yahoo.com 18, 40 -- Received: (qmail 26517 invoked by uid 60001); 8 Feb 2012 10:18:03 -0000 18, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1328696283; bh=QW5iWPJB73GM0z4BtsjljDztevyNVfLi10zWfPeUaP8=; h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=272dij64hq95Irdxe5SnyPzlEeA0DL3qmjAkF7eDBdnXzsgTxB6AS2wvkxhDTZZsCfXvwvSm+b3zUWrUaI3Uc/RqdOL6jLZvQ2whFCKlUHs6jzxqhSuTDcQtZd87aRz/KUE6HdTERgVeNFdNj+8PnfHaa/8tgzyotHFIhlzDKA0= 18, 40 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 18, 40 -- s=s1024; d=yahoo.com; 18, 40 -- h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 18, 40 -- b=VxePvQkISi/zk6guO9qgkreoAqbzSI7krucoaKTtvAO8yHh6HabShAI3uXrnnP8QL/CNJjGoxpi1eHnHZWnp1pn2LQmlm2fm8xMfiPIii5sjanHSro5VOhZpXUN2ENKKXyOy0KCbPnhyaAA3DK3G7xz7uIGKc7TpdkblpTAi7iA=; 18, 40 -- X-YMail-OSG: VtkSjxIVM1kBkv5jxIMnCLYIZx_P0OFq9AEkWu5o1d_hOjm 18, 40 -- mT2sYb55.QMlZOz2.VsMiQcVdqAFj0c6zu3PpwKZzpj2TkIzi8iUqXxzmUQV 18, 40 -- a.7v8V0NHG3bhOyB1m4R2e7.Mg6ANbl3RBQFeFCm0Nj9XRmxVIvq6eY1AeSv 18, 40 -- kA83vt3ifIQUC5fC7oqSNGANnn5rMA01mAI5FkgJGYoOnuf.5V4QoykLFZXr 18, 40 -- T6Qx31VWI9aeEeYF9dZp8KqyiyK3aCUa7jrB4YfqyGIChZjBPZSdabDZ.wHd 18, 40 -- IfRJj9K6h5Mv.ryd8JonvM6nlKjE_dD3Z._9fcXrVcFxafyd7uk2RcdneDe7 18, 40 -- B8Db9L3dZRzmqWy32IB6wGRvuRoecPuA69YDeyMwX9CZYtJIKBn5_hkguxNv 18, 40 -- wqp6q80X6JP6KDqMct7KA3nstX.gAOzhM.1dsYncJjrQl3S4bhbFDW4g6NO7 18, 40 -- GiuhtocEVdmHrnyamQfsfAu0QjXXIcqEcsxjYo0El8WZ6DBtuIZFt_Zje3fv 18, 40 -- kTgVyZPqxiZdaRYPTs4t.f4u1P5VhYPWXm0bqQQ_qxTRp2C7TfYmY5OyvEI2 18, 40 -- YJyqawBAsRg-- 18, 40 -- Received: from [80.122.101.100] by web110812.mail.gq1.yahoo.com via HTTP; Wed, 08 Feb 2012 02:18:03 PST 18, 40 -- X-Mailer: YahooMailWebService/0.8.116.338427 18, 40 -- References: {201202071733.q17HXcnr011326-at-ns.microscopy.com} 18, 40 -- Message-ID: {1328696283.16185.YahooMailNeo-at-web110812.mail.gq1.yahoo.com} 18, 40 -- Date: Wed, 8 Feb 2012 02:18:03 -0800 (PST) 18, 40 -- From: Stephane Nizet {nizets2-at-yahoo.com} 18, 40 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 18, 40 -- Subject: Re: [Microscopy] TEM/AFM and nanoparticles 18, 40 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 18, 40 -- In-Reply-To: {201202071733.q17HXcnr011326-at-ns.microscopy.com} 18, 40 -- MIME-Version: 1.0 18, 40 -- Content-Type: text/plain; charset=iso-8859-1 18, 40 -- Content-Transfer-Encoding: 8bit 18, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q18AI4PX009989 ==============================End of - Headers==============================
This conundrum you indicate is like life. One picks his or her poison according to the needs, goals, facilities available, time, and economic/collaborative capabilities. Sometimes an association is all the information that is needed to answer a question. All of the methods available today for visualizing proteins have flaws. Negative stain EM is a good first start with other techniques such as cryo-EM and AFM later based on what was learned by "crude" negative stain EM, if indeed one is attempting to study how proteins are organized around a matrix.
Best regards,
Charles Humphrey, Ph.D. CDC Atlanta, GA 30333
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Wednesday, February 08, 2012 5:28 AM To: Humphrey, Charles (CDC/OID/NCEZID)
I already gave my opinion to Marie about this offline but since there were several answers in the list going in this direction I would like to share some thoughts with the list.
Negative staining may work. This means: you may see your proteins, you may get a result. You may even get a nice picture. But hey, are we artists or are we scientists? Our job is not to get nice pictures, it is to get meaningful pictures.
Marie wants to know how the antibodies (or other proteins) are organized around gold particles in a matrix, most probably water or aqueous solution. I am not sure the best way to know that is by drying the sample with a heavy stain. Do you really think it may not interfere with the binding forces between the antibody and the gold particle? I wouldn't bet a cent on it!
Regards, Stephane
----- Original Message ----- X-from: "petereaton-at-hotmail.com" {petereaton-at-hotmail.com} To: nizets2-at-yahoo.com Cc: Sent: Tuesday, February 7, 2012 6:33 PM
On the other hand, there is no doubt that if the negative staining works well, and you can image both the the AuNPs and proteins, it will be a nicer result, and probably less time consuming.
Good Luck!
Pete.
==============================Original Headers============================== 11, 20 -- From petereaton-at-hotmail.com Tue Feb 7 11:30:56 2012 11, 20 -- Received: from snt0-omc3-s23.snt0.hotmail.com (snt0-omc3-s23.snt0.hotmail.com [65.55.90.162]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q17HUuKI005821 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2012 11:30:56 -0600 11, 20 -- Received: from SNT139-W62 ([65.55.90.136]) by snt0-omc3-s23.snt0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 11, 20 -- Tue, 7 Feb 2012 09:30:56 -0800 11, 20 -- Message-ID: {SNT139-W62F773C3410166EDB29535C8750-at-phx.gbl} 11, 20 -- X-Originating-IP: [193.136.24.67] 11, 20 -- From: Peter Eaton {petereaton-at-hotmail.com} 11, 20 -- To: {microscopy-at-microscopy.com} 11, 20 -- Subject: TEM/AFM and nanoparticles 11, 20 -- Date: Tue, 7 Feb 2012 17:30:55 +0000 11, 20 -- Importance: Normal 11, 20 -- In-Reply-To: {SNT139-W67102750CFDD569369865C8750-at-phx.gbl} 11, 20 -- References: {SNT139-W67102750CFDD569369865C8750-at-phx.gbl} 11, 20 -- Content-Type: text/plain; charset="Windows-1252" 11, 20 -- MIME-Version: 1.0 11, 20 -- X-OriginalArrivalTime: 07 Feb 2012 17:30:56.0268 (UTC) FILETIME=[3FBB2CC0:01CCE5BE] 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q17HUuKI005821 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 40 -- From nizets2-at-yahoo.com Wed Feb 8 04:18:05 2012 18, 40 -- Received: from nm14-vm1.bullet.mail.ne1.yahoo.com (nm14-vm1.bullet.mail.ne1.yahoo.com [98.138.91.38]) 18, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q18AI4PX009989 18, 40 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 04:18:05 -0600 18, 40 -- Received: from [98.138.90.57] by nm14.bullet.mail.ne1.yahoo.com with NNFMP; 08 Feb 2012 10:18:04 -0000 18, 40 -- Received: from [98.138.89.244] by tm10.bullet.mail.ne1.yahoo.com with NNFMP; 08 Feb 2012 10:18:04 -0000 18, 40 -- Received: from [127.0.0.1] by omp1058.mail.ne1.yahoo.com with NNFMP; 08 Feb 2012 10:18:04 -0000 18, 40 -- X-Yahoo-Newman-Property: ymail-3 18, 40 -- X-Yahoo-Newman-Id: 260981.33175.bm-at-omp1058.mail.ne1.yahoo.com 18, 40 -- Received: (qmail 26517 invoked by uid 60001); 8 Feb 2012 10:18:03 -0000 18, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1328696283; bh=QW5iWPJB73GM0z4BtsjljDztevyNVfLi10zWfPeUaP8=; h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=272dij64hq95Irdxe5SnyPzlEeA0DL3qmjAkF7eDBdnXzsgTxB6AS2wvkxhDTZZsCfXvwvSm+b3zUWrUaI3Uc/RqdOL6jLZvQ2whFCKlUHs6jzxqhSuTDcQtZd87aRz/KUE6HdTERgVeNFdNj+8PnfHaa/8tgzyotHFIhlzDKA0= 18, 40 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 18, 40 -- s=s1024; d=yahoo.com; 18, 40 -- h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 18, 40 -- b=VxePvQkISi/zk6guO9qgkreoAqbzSI7krucoaKTtvAO8yHh6HabShAI3uXrnnP8QL/CNJjGoxpi1eHnHZWnp1pn2LQmlm2fm8xMfiPIii5sjanHSro5VOhZpXUN2ENKKXyOy0KCbPnhyaAA3DK3G7xz7uIGKc7TpdkblpTAi7iA=; 18, 40 -- X-YMail-OSG: VtkSjxIVM1kBkv5jxIMnCLYIZx_P0OFq9AEkWu5o1d_hOjm 18, 40 -- mT2sYb55.QMlZOz2.VsMiQcVdqAFj0c6zu3PpwKZzpj2TkIzi8iUqXxzmUQV 18, 40 -- a.7v8V0NHG3bhOyB1m4R2e7.Mg6ANbl3RBQFeFCm0Nj9XRmxVIvq6eY1AeSv 18, 40 -- kA83vt3ifIQUC5fC7oqSNGANnn5rMA01mAI5FkgJGYoOnuf.5V4QoykLFZXr 18, 40 -- T6Qx31VWI9aeEeYF9dZp8KqyiyK3aCUa7jrB4YfqyGIChZjBPZSdabDZ.wHd 18, 40 -- IfRJj9K6h5Mv.ryd8JonvM6nlKjE_dD3Z._9fcXrVcFxafyd7uk2RcdneDe7 18, 40 -- B8Db9L3dZRzmqWy32IB6wGRvuRoecPuA69YDeyMwX9CZYtJIKBn5_hkguxNv 18, 40 -- wqp6q80X6JP6KDqMct7KA3nstX.gAOzhM.1dsYncJjrQl3S4bhbFDW4g6NO7 18, 40 -- GiuhtocEVdmHrnyamQfsfAu0QjXXIcqEcsxjYo0El8WZ6DBtuIZFt_Zje3fv 18, 40 -- kTgVyZPqxiZdaRYPTs4t.f4u1P5VhYPWXm0bqQQ_qxTRp2C7TfYmY5OyvEI2 18, 40 -- YJyqawBAsRg-- 18, 40 -- Received: from [80.122.101.100] by web110812.mail.gq1.yahoo.com via HTTP; Wed, 08 Feb 2012 02:18:03 PST 18, 40 -- X-Mailer: YahooMailWebService/0.8.116.338427 18, 40 -- References: {201202071733.q17HXcnr011326-at-ns.microscopy.com} 18, 40 -- Message-ID: {1328696283.16185.YahooMailNeo-at-web110812.mail.gq1.yahoo.com} 18, 40 -- Date: Wed, 8 Feb 2012 02:18:03 -0800 (PST) 18, 40 -- From: Stephane Nizet {nizets2-at-yahoo.com} 18, 40 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 18, 40 -- Subject: Re: [Microscopy] TEM/AFM and nanoparticles 18, 40 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 18, 40 -- In-Reply-To: {201202071733.q17HXcnr011326-at-ns.microscopy.com} 18, 40 -- MIME-Version: 1.0 18, 40 -- Content-Type: text/plain; charset=iso-8859-1 18, 40 -- Content-Transfer-Encoding: 8bit 18, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q18AI4PX009989 ==============================End of - Headers==============================
==============================Original Headers============================== 29, 35 -- From cdh1-at-cdc.gov Wed Feb 8 08:33:48 2012 29, 35 -- Received: from mail126.messagelabs.com (mail126.messagelabs.com [216.82.250.99]) 29, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q18EXlji031559 29, 35 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 08:33:48 -0600 29, 35 -- X-Env-Sender: cdh1-at-cdc.gov 29, 35 -- X-Msg-Ref: server-4.tower-126.messagelabs.com!1328711624!45680006!3 29, 35 -- X-Originating-IP: [158.111.54.26] 29, 35 -- X-StarScan-Version: 6.5.5; banners=-,-,- 29, 35 -- X-VirusChecked: Checked 29, 35 -- Received: (qmail 17894 invoked from network); 8 Feb 2012 14:33:47 -0000 29, 35 -- Received: from unknown (HELO ECASHUB-CLFT2.cdc.gov) (158.111.54.26) 29, 35 -- by server-4.tower-126.messagelabs.com with AES128-SHA encrypted SMTP; 8 Feb 2012 14:33:47 -0000 29, 35 -- Received: from EMBX-CLFT2.cdc.gov ([fe80::4cc3:f3c7:6959:3e75]) by 29, 35 -- ECASHUB-CLFT2.cdc.gov ([158.111.54.26]) with mapi id 14.01.0355.002; Wed, 8 29, 35 -- Feb 2012 09:33:45 -0500 29, 35 -- From: "Humphrey, Charles (CDC/OID/NCEZID)" {cdh1-at-cdc.gov} 29, 35 -- To: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} , 29, 35 -- "microscopy-at-microscopy.com" 29, 35 -- {microscopy-at-microscopy.com} 29, 35 -- Subject: RE: [Microscopy] Re: TEM/AFM and nanoparticles 29, 35 -- Thread-Topic: [Microscopy] Re: TEM/AFM and nanoparticles 29, 35 -- Thread-Index: AQHM5kxd2pF3f1OztUGHX+obwwWT/pYzCojA 29, 35 -- Date: Wed, 8 Feb 2012 14:33:45 +0000 29, 35 -- Message-ID: {231981A6C34E2B4589ADAC40FC813A62182EA56E-at-EMBX-CLFT2.cdc.gov} 29, 35 -- References: {201202081028.q18ASCLh023268-at-ns.microscopy.com} 29, 35 -- In-Reply-To: {201202081028.q18ASCLh023268-at-ns.microscopy.com} 29, 35 -- Accept-Language: en-US 29, 35 -- Content-Language: en-US 29, 35 -- X-MS-Has-Attach: 29, 35 -- X-MS-TNEF-Correlator: 29, 35 -- x-originating-ip: [158.111.190.193] 29, 35 -- Content-Type: text/plain; charset="iso-8859-1" 29, 35 -- MIME-Version: 1.0 29, 35 -- Content-Transfer-Encoding: 8bit 29, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q18EXlji031559 ==============================End of - Headers==============================
We have taken almost all the shielding from our Tecnai G20 down in order to make some repairs and now that I am ready to mount them again, I ask myself: Should I ask FEI to come and control the X-radiation? We work mostly at 100-120kV but sometimes we use 200kV so X-rays should be considered, but is there a risk of leak after unmounting/remounting the shielding? Thanks in advance for sharing your thoughts.
Regards, Stephane
==============================Original Headers============================== 3, 36 -- From nizets2-at-yahoo.com Wed Feb 8 09:57:43 2012 3, 36 -- Received: from nm21-vm0.bullet.mail.sp2.yahoo.com (nm21-vm0.bullet.mail.sp2.yahoo.com [98.139.91.220]) 3, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id q18Fvg8P030661 3, 36 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 09:57:42 -0600 3, 36 -- Received: from [98.139.91.70] by nm21.bullet.mail.sp2.yahoo.com with NNFMP; 08 Feb 2012 15:57:42 -0000 3, 36 -- Received: from [98.139.91.25] by tm10.bullet.mail.sp2.yahoo.com with NNFMP; 08 Feb 2012 15:57:42 -0000 3, 36 -- Received: from [127.0.0.1] by omp1025.mail.sp2.yahoo.com with NNFMP; 08 Feb 2012 15:57:42 -0000 3, 36 -- X-Yahoo-Newman-Property: ymail-3 3, 36 -- X-Yahoo-Newman-Id: 280678.77931.bm-at-omp1025.mail.sp2.yahoo.com 3, 36 -- Received: (qmail 78942 invoked by uid 60001); 8 Feb 2012 15:57:41 -0000 3, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1328716661; bh=tfCyCgoH0RjEoOw1UEGIuRwczlvn/Ji/6cQHSstYcyc=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=4Q0TKXlxm5kMjnb1KKSNG7albxqh9Sy6j9apAtK6vHkC2Ogfl4AhRV8Z6dzQqWCB8FhoymQ2ftGXftl/AGGDa6tZimzoSyr2ONtFHf9ZIhZlQUdxi10blQwqnXy2zF9i/9ctzTZYMFsjFvQc7PahxqqWyG8K6uD9WzgYR4X/eOw= 3, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 36 -- s=s1024; d=yahoo.com; 3, 36 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 3, 36 -- b=Y7CgWsqA/Xljm1J3uz8FVPRbfSL03uRw20VxLE+tp+j72OU0zliCYzKONYCrzrdnrvGXEcVPW/K+cKDyzkdTzMnJHryuEBX8hAn315PpJRsTPO7Ym+w/E2f5Vzmto5h0VTCMXYx7n+WuMVMUcqGKT/oh5CTn/EFMH5qwRer4Kl8=; 3, 36 -- X-YMail-OSG: 3qcCUU4VM1mZnZxhi.U2A4QUnX53.N2hwPzT4PH295LDngE 3, 36 -- l5tPfrhjmkN2W_3bUg6ZEerXXQRjEP.lqSu__XEILHqOCiKQuio9LSibVdSW 3, 36 -- aPwtsa_zdezt_Swuzk.hQwd4SqTTjW6q.1U1FsfDgAKRRQFeI8vLgR7C.uXj 3, 36 -- 1vI.wHKdwySdQety.g.N7Fv2E3t6yR9TrbyfL_B_OlICqopkcQMjmnpbTom_ 3, 36 -- w7rV.e_kUkgVD6QfX9dxp25Is5EXhwCeQsKZBaOvuaj7u0k3mt5qZ_PbNVfr 3, 36 -- nQkjQb1Iv9al9OmaqcANZyGPTki9Xmz8mtJhO2tpjrahLq58zvBqUgCII0bA 3, 36 -- SDv6CFO4GqVXCe_4l.WGk0tjp0Vdr_Qy7ZZ9cIJjnzpvWg3TlGVdkD81Z84R 3, 36 -- NIEu_SW86ncY2aZmt.935M.i4UL00f_c9vA7sgnSFYhijBsxcniYbMOA3BVF 3, 36 -- BLs4- 3, 36 -- Received: from [80.122.101.100] by web110807.mail.gq1.yahoo.com via HTTP; Wed, 08 Feb 2012 07:57:41 PST 3, 36 -- X-Mailer: YahooMailWebService/0.8.116.338427 3, 36 -- Message-ID: {1328716661.56373.YahooMailNeo-at-web110807.mail.gq1.yahoo.com} 3, 36 -- Date: Wed, 8 Feb 2012 07:57:41 -0800 (PST) 3, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 36 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 36 -- Subject: X ray control after dismounting a TEM 3, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 36 -- MIME-Version: 1.0 3, 36 -- Content-Type: text/plain; charset=iso-8859-1 3, 36 -- Content-Transfer-Encoding: 8bit 3, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q18Fvg8P030661 ==============================End of - Headers==============================
Thanks to everyone who has responded to my question. Since there seemed to be quite a bit of interest, I will summarize what I've discovered so far (I hope those who responded off-line will not mind being paraphrased, and my apologies if I accidentally omitted your suggestions. There were a lot of emails.)
Several people suggested publications, and I did additional searches on my own (though not exhaustive). Google Scholar proved to be more useful than Pubmed, perhaps because many of these papers are in chemistry journals.
Here are some papers that include EM methods (noted in parentheses), in no particular order:
Transmission Electron Microscopy as a Tool to Image Bioinorganic Nanohybrids: The Case of Phage-Gold Nanocomposites MICROSCOPY RESEARCH AND TECHNIQUE 74:627–635 (2011) (negative staining, mainly of larger protein assemblies)
Apoferritin-Templated Synthesis of Encoded Metallic Phosphate Nanoparticle Tags. Anal. Chem. 2007, 79, 5614-5619. (TEM unstained and negative stained).
Direct Imaging of Soft-Hard Interfaces Enabled by Graphene. Nano Letters 2009 Vol 9 No 9 3365-3369 (very cool images using high resolution TEM and substrate, unstained)
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles. SCIENCE VOL 298 29 NOVEMBER 2002 1759-1762 (negative stained)
Electron Density Imaging of Protein Films on Gold-Particle Surfaces With Transmission Electron Microscopy. Ludger Ju¨rgens,* Alfons Nichtl, and Ulf Werner. Cytometry 37:87–92 (1999) (TEM, unstained and ruthenium stained prior to conjugation)
Controlled Encapsidation of Gold Nanoparticles by a Viral Protein Shell. LiNa Loo, Richard H. Guenther, Veronica R. Basnayake, Steven A. Lommel, and Stefan Franzen*. J. AM. CHEM. SOC. 2006, 128, 4502-4503 (negative stain TEM and possibly cryo, but results not shown)
A fluorescent, paramagnetic and PEGylated gold/silica nanoparticle for MRI, CT and fluorescence imaging Matti M. van Schoonevelda, David P. Cormodeb, Rolf Koole, J. Timon van Wijngaardena, Claudia Calcagno, Torjus Skajaa, Jan Hilhorst, Dannis C. ’t Hartc, Zahi A. Fayadb, Willem J. M. Mulder* and Andries Meijerinka. Contrast Media Mol. Imaging 2010, 5 231–236 2010. (negative and positive stain)
DNA-Origami-Directed Self-Assembly of Discrete Silver-Nanoparticle Architectures. Suchetan Pal, Zhengtao Deng, Baoquan Ding, Hao Yan,* and Yan Liu. Angew. Chem. 2010, 122, 2760 –2764 (negative stain w/ uranyl formate and unstained with STEM; not typical gold nanoparticles, but pretty interesting structures!)
New Frontiers in Gold Labeling. James F. Hainfeld and Richard D. Powell The Journal of Histochemistry & Cytochemistry 48(4): 471–480, 2000 (STEM images of nanogold labelled Fab)
Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells Ai Leen Koh , Catherine M. Shachaf, Sailaja Elchuri, Garry P. Nolan, Robert Sinclair. Ultramicroscopy 109 (2008) 111–121 (negative stain TEM, SEM, BSE, Quantomix SEM and EDS)
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles Benoit Dubertret, Paris Skourides, David J. Norris, Vincent Noireaux, Ali H. Brivanlou, Albert Libchaber. SCIENCE VOL 298 29 NOVEMBER2002 (negative stain TEM)
Methods suggested:
-Negative staining, Uranyl acetate (0.5-2%) or ammonium molybdate (1-2% w/trahalose), preferably with FEG or LaB6. Use unfunctionalized particles as a control. Use of trehalose or an embedding medium such as methyl cellulose was also suggested (see Methods in Molecular Biology 369 Electron Microscopy: Mehods and Protocols, 2nd edition, Chapter 7 by Robin Harris). In general, drying artifacts such as flattening are likely to be observed with negative staining, but use of these embedments will reduce this.
-Use of graphene support films see paper above in Nano Letters.
-dynamic light scattering
-cryo TEM or cryo FESEM were suggested
-Pt/C shadowing was suggested
-AFM. The dimension of the coating can be estimated by comparing the height of the coated and uncoated particles.
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 25, 19 -- From marie.cantino-at-uconn.edu Wed Feb 8 13:19:25 2012 25, 19 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 25, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q18JJPWI025409 25, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 8 Feb 2012 13:19:25 -0600 25, 19 -- Received: from d47h9.public.uconn.edu (d47h9.public.uconn.edu [137.99.47.9]) 25, 19 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 179052CD64 25, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 8 Feb 2012 14:19:25 -0500 (EST) 25, 19 -- Message-Id: {5CF3C858-B762-4FED-8355-42C658D2E04F-at-uconn.edu} 25, 19 -- From: Marie Cantino {marie.cantino-at-uconn.edu} 25, 19 -- To: Microscopy-at-Microscopy.Com 25, 19 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes 25, 19 -- Mime-Version: 1.0 (Apple Message framework v936) 25, 19 -- Subject: RE: [Microscopy] TEM of functionalized nanoparticles 25, 19 -- Date: Wed, 8 Feb 2012 14:19:24 -0500 25, 19 -- X-Mailer: Apple Mail (2.936) 25, 19 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 25, 19 -- X-Virus-Status: Clean 25, 19 -- Content-Transfer-Encoding: 8bit 25, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q18JJPWI025409 ==============================End of - Headers==============================
Our client wants us to image proteins using SEM. The protein is dissolved in water and currently stored at -20degrees C. What do you suggest? This is what I'm thinking:
Float a formvar-coated grid on drop of solution or place a drop of solution on a poly-L-lysine cover slip.
Fix grid or cover slip.
Rinse.
Osmicate grid.
Rinse and dry.
Sputter coat.
Thank you very much,
Jaci
Jaclynn Lett Senior Research Technician Research Center for Auditory and Visual Studies Washington University School Of Medicine Saint Louis, Missouri lettj-at-ent.wustl.edu
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
In my quest for an affordable printer to make B+W images of carbon black particles, I floated this question to the microscopy list server: "What's going to give me the nicest B+W TEM print?" After all, the images included in a report may be all the client knows about you.
I got a few interesting answers and in the process learned a bit about printers. But first, here's what I'm currently using: an HP2550 with copier paper. By the way the noise as stopped and the printer continues to run fine.
Here's what was suggested to me: HP C5180, HP2300, HP8750, Epson 800, Sony Medical Printers.
Most people felt a color printer gave better gray scale images than a simple B+W printer but I was cautioned that a single black ink cartridge would be unable to create the entire tonal shading that good B+W prints need. Two different black ink cartridges could accomplish this.
Everyone had their own favorite printer and of course visiting vendor websites at 72 lines per inch of monitor resolution makes everyone's images look good. The best advice I got was to print images on as many printers as possible and then decide.
As a personal note, I'm not going to shop for printers right now, but I will try a ream of a better paper. Since several people print both text and images on our printer, running photo quality paper isn't an answer we can afford.
Increasing the resolution of our TEM camera would also be a big help,......
Thanks again for everyone's advice.
Frank
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 19, 25 -- From frank_karl-at-ardl.com Wed Feb 8 14:27:31 2012 19, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q18KRSrI027930 19, 25 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 14:27:29 -0600 19, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 19, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Wed, 8 Feb 19, 25 -- 2012 15:27:27 -0500 19, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 19, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 19, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 19, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 19, 25 -- Date: Wed, 8 Feb 2012 15:27:26 -0500 19, 25 -- Subject: B+W printer response 19, 25 -- Thread-Topic: B+W printer response 19, 25 -- Thread-Index: AczmoBJ2F6qmDtz2Sf6paqUT+T+eNg== 19, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF1507710F8BE1FDD-at-exchange2k7.ad.ardl.com} 19, 25 -- Accept-Language: en-US 19, 25 -- Content-Language: en-US 19, 25 -- X-MS-Has-Attach: 19, 25 -- X-MS-TNEF-Correlator: 19, 25 -- acceptlanguage: en-US 19, 25 -- Content-Type: text/plain; charset="iso-8859-1" 19, 25 -- MIME-Version: 1.0 19, 25 -- Content-Transfer-Encoding: 8bit 19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id q18KRSrI027930 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both slakmon-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I was hoping to get people's feedback and opinion on the different diamond knives on the market. Your opinion on new, resharpened would be helpful as well. What methods that you found best suited in cleaning your knives without damaging your knives. Please feel free to respond on the group or offline directly to my email address.
HI Philip- I have tried knives from different sources but have always come back to Diatome. New or resharpened, they are great. The HIsto knife is wonderful for 0.5-2 um sections. I haven't used glass in years. The ultras are great from end-to-end. You also get great customer support from both the US and Swiss offices. Lee Cohen-Gould, M.S., C.E.M.T. Chair, MSA Certification Board
Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
On Feb 8, 2012, at 4:46 PM, microscopylistserver-noreply-at-microscopy.com wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both slakmon-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I was hoping to get people's feedback and opinion on the different diamond knives on the market. Your opinion on new, resharpened would be helpful as well. What methods that you found best suited in cleaning your knives without damaging your knives. Please feel free to respond on the group or offline directly to my email address.
________________________________________ } From: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Friday, February 03, 2012 10:47 AM To: Monson, Frederick
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mary.walker-at-csl.com.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mary.walker-at-csl.com.au Name: mary walker
Message: Do any listers have experience with counting virus particles with latex beads? Is it possible to do better than order of magnitude? What is the minimum number of particles per ml that can be counted? What is the lower limit?
I have done my fair share of virus particle counts over too many decades and have some opinions about the process. Latex beads may be used in counting but I would not recommend them as a standard except as a rough size standard. The manufacturer of the beads would agree as well I think. It is a supreme leap of faith to believe that beads adsorb and remain adsorbed to formvar-carbon films or any other films in the same manner as viruses. I also expect different viruses passively adsorb differently as well. If you are using airfuge techniques the adsorption issue may be somewhat improved but one still has to stain and assume beads remain adsorbed to the grids exactly as the viruses. I know there are many publications that have used latex beads as counting standards but my understanding of viruses causes me to be skeptical of including latex beads for counting. One can count the viruses directly using the grid squares as a constant and then extrapolate, or even better with digital images a digital image grid of precise size can be overlaid and is helpful.
It is also risky to consider reliability beyond 10 -fold. My experience is that there is considerable variability within the 10-fold limits. I suspect this is true with most counting methods; EM or otherwise. The major advantage with EM is that one can actually see aggregates when they occur (another issue with virus counts if other counting methods are used).
If one assumes on a 300 mesh grid with a grid hole of 61 microns, 20 particles within that grid square would represent approximately 107 particles per ml. Therefore, considering the fields one would have to view and identify particles, then probably 106 particles per ml is about the limit one would wish to search for counting. This is what I would consider as a lower practical limit. One could improve on the lower limit by looking at many-many grid squares but that would require considerable searching, patience, and time.
I hope this is helpful; if you have additional questions I may be reached by email or my address below.
Happy counting,
Charles Humphrey Mailstop G32 US Centers for Disease Control and Prevention (CDC) Atlanta, GA 30333
-----Original Message----- } From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, February 03, 2012 10:49 AM To: Humphrey, Charles (CDC/OID/NCEZID)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mary.walker-at-csl.com.au {mailto:mary.walker-at-csl.com.au} as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mary.walker-at-csl.com.au {mailto:mary.walker-at-csl.com.au} Name: mary walker
Message: Do any listers have experience with counting virus particles with latex beads? Is it possible to do better than order of magnitude? What is the minimum number of particles per ml that can be counted? What is the lower limit?
==============================Original Headers============================== 9, 25 -- From microscopylistserver-noreply-at-microscopy.com {mailto:microscopylistserver-noreply-at-microscopy.com} Fri Feb 3 09:35:54 2012 9, 25 -- Received: from znl.com ([206.69.208.20]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q13FZs77015955 9, 25 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 3 Feb 2012 09:35:54 -0600 9, 25 -- Received: from localhost (localhost [127.0.0.1]) 9, 25 -- by znl.com (Postfix) with ESMTP id 9B984485144 9, 25 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 3 Feb 2012 09:35:54 -0600 (CST) 9, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 9, 25 -- Received: from znl.com ([127.0.0.1]) 9, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 9, 25 -- with ESMTP id GA2KYCBcTT6V for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; 9, 25 -- Fri, 3 Feb 2012 09:35:54 -0600 (CST) 9, 25 -- Received: from NestorPwrBk.local (dsl-58-6-4-117.wa.westnet.com.au [58.6.4.117]) 9, 25 -- by znl.com (Postfix) with ESMTPA id 858CD485134 9, 25 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 3 Feb 2012 09:35:53 -0600 (CST) 9, 25 -- Message-ID: {4F2BFED4.7070106-at-microscopy.com {mailto:4F2BFED4.7070106-at-microscopy.com} } 9, 25 -- Date: Fri, 03 Feb 2012 23:35:48 +0800 9, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com {mailto:microscopylistserver-noreply-at-microscopy.com} } 9, 25 -- Reply-To: mary.walker-at-csl.com.au {mailto:mary.walker-at-csl.com.au} 9, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 9, 25 -- MIME-Version: 1.0 9, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } 9, 25 --
Hi Ravi,
It could be that your sample was not at the 'standard focus' height. Set your objective lens to the 'standard focus' value and then move your sample height to get to focus.
I hope this helps.
Ke-Bin
microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both ravi.thakkar369-at-gmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com Name: Ravindra Thakkar } } Title-Subject: [Filtered] TEM : Focusing Problem. } } Message: Image is not getting focused. The image is drastically moving } while focusing, it moving highly even at low focus step & at low } magnification. } } So how to resolve this problem. } } Login Host: 14.139.97.76 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Feb 6 08:08:03 2012 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q16E83SK024773 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 08:08:03 -0600 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id 19AF8487506 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 08:08:03 -0600 (CST) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 8, 25 -- with ESMTP id 47U5ZZGt8Bky for {microscopy-at-microscopy.com} ; } 8, 25 -- Mon, 6 Feb 2012 08:07:55 -0600 (CST) } 8, 25 -- Received: from NestorPwrBk.local (dsl-58-6-20-170.wa.westnet.com.au [58.6.20.170]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id 5BFB34874F6 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2012 08:07:55 -0600 (CST) } 8, 25 -- Message-ID: {4F2FDEB8.7090909-at-microscopy.com} } 8, 25 -- Date: Mon, 06 Feb 2012 22:07:52 +0800 } 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: ravi.thakkar369-at-gmail.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: [Filtered] viaWWW:TEM : Focusing Problem. } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 27 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 8 16:59:48 2012 7, 27 -- Received: from znl.com ([206.69.208.20]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q18Mxm2u006711 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 16:59:48 -0600 7, 27 -- Received: from localhost (localhost [127.0.0.1]) 7, 27 -- by znl.com (Postfix) with ESMTP id 3F924489135 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 16:59:48 -0600 (CST) 7, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 27 -- Received: from znl.com ([127.0.0.1]) 7, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 27 -- with ESMTP id aDgd8840+hf9 for {microscopy-at-microscopy.com} ; 7, 27 -- Wed, 8 Feb 2012 16:59:46 -0600 (CST) 7, 27 -- Received: from NestorPwrBk.local (dsl-58-6-20-170.wa.westnet.com.au [58.6.20.170]) 7, 27 -- by znl.com (Postfix) with ESMTPA id A90EC48912A 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 16:59:45 -0600 (CST) 7, 27 -- Message-ID: {4F32FE5F.40407-at-microscopy.com} 7, 27 -- Date: Thu, 09 Feb 2012 06:59:43 +0800 7, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 27 -- Reply-To: Ke-Bin Low {kebinlow-at-uic.edu} 7, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 27 -- MIME-Version: 1.0 7, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 27 -- Subject: [Filtered] Re: [Microscopy] viaWWW:TEM : Focusing Problem 7, 27 -- References: {201202061416.q16EGNnJ001781-at-ns.microscopy.com} 7, 27 -- In-Reply-To: {201202061416.q16EGNnJ001781-at-ns.microscopy.com} 7, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, I am looking for someone old enough to be fit in Visual Basic 6 ;-) to help me with an existing VB6 program to remote my Philips 525 SEM. Knowledge of German language would be perfect... If interested, please contact me offline.
Best wishes, Stefan --
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
My experience with the Histo knife is similar. Its an incredible knife!
Dr. Gary B. Carr Pacific Endodontic Research Foundation San Diego, CA 92121 ----- Original Message ----- } From: {microscopylistserver-noreply-at-microscopy.com} To: {gary-at-perfendo.com} Sent: Wednesday, February 08, 2012 3:08 PM
I would request that you respond on-line. I would be interested in others' views.
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood-at-partners.org
-----Original Message----- } From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, February 08, 2012 4:45 PM To: Sherwood, Margaret
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both slakmon-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I was hoping to get people's feedback and opinion on the different diamond knives on the market. Your opinion on new, resharpened would be helpful as well. What methods that you found best suited in cleaning your knives without damaging your knives. Please feel free to respond on the group or offline directly to my email address.
==============================Original Headers============================== 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 8 15:39:16 2012 10, 25 -- Received: from znl.com ([206.69.208.20]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q18LdGu8014370 10, 25 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 15:39:16 -0600 10, 25 -- Received: from localhost (localhost [127.0.0.1]) 10, 25 -- by znl.com (Postfix) with ESMTP id 14952488FC2 10, 25 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 15:39:16 -0600 (CST) 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 25 -- Received: from znl.com ([127.0.0.1]) 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 25 -- with ESMTP id wM9-bsUVG8EG for {microscopy-at-microscopy.com} ; 10, 25 -- Wed, 8 Feb 2012 15:39:12 -0600 (CST) 10, 25 -- Received: from NestorPwrBk.local (dsl-58-6-20-170.wa.westnet.com.au [58.6.20.170]) 10, 25 -- by znl.com (Postfix) with ESMTPA id 5AFE8488FB6 10, 25 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2012 15:39:12 -0600 (CST) 10, 25 -- Message-ID: {4F32EB81.2090503-at-microscopy.com} 10, 25 -- Date: Thu, 09 Feb 2012 05:39:13 +0800 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 25 -- Reply-To: slakmon-at-yahoo.com 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 25 -- MIME-Version: 1.0 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 25 -- Subject: [Filtered] viaWWW:Diamond knives & cleaning methods 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.
==============================Original Headers============================== 23, 27 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 9 02:21:22 2012 23, 27 -- Received: from znl.com ([206.69.208.20]) 23, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id q198LMRw002195 23, 27 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2012 02:21:22 -0600 23, 27 -- Received: from localhost (localhost [127.0.0.1]) 23, 27 -- by znl.com (Postfix) with ESMTP id 74D6D48963D 23, 27 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2012 02:21:22 -0600 (CST) 23, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 23, 27 -- Received: from znl.com ([127.0.0.1]) 23, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 23, 27 -- with ESMTP id L2HD5U1tMvk2 for {microscopy-at-microscopy.com} ; 23, 27 -- Thu, 9 Feb 2012 02:21:21 -0600 (CST) 23, 27 -- Received: from NestorPwrBk.local (dsl-58-6-20-170.wa.westnet.com.au [58.6.20.170]) 23, 27 -- by znl.com (Postfix) with ESMTPA id 9D97A489632 23, 27 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2012 02:21:19 -0600 (CST) 23, 27 -- Message-ID: {4F3381FD.4010609-at-microscopy.com} 23, 27 -- Date: Thu, 09 Feb 2012 16:21:17 +0800 23, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 23, 27 -- Reply-To: "Sherwood, Margaret" {MSHERWOOD-at-PARTNERS.ORG} 23, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 23, 27 -- MIME-Version: 1.0 23, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 23, 27 -- Subject: [Filtered] RE: [Microscopy] viaWWW:Diamond knives cleaning methods 23, 27 -- References: {201202082144.q18Lisfc024197-at-ns.microscopy.com} 23, 27 -- In-Reply-To: {201202082144.q18Lisfc024197-at-ns.microscopy.com} 23, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 23, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I'd have thought the osmium would be an unnecessary step since you're coating the specimen before viewing. To fix the protein I'd be inclined to use glutaraldehyde vapour - we've fixed problematic complexes before by blotting the grid then holding it in the air space in a bottle of the fixative to ten to thirty seconds. After a quick rinse you ought to be able to dry it and put straight in the coater. Cheers Ian
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: 08 February 2012 19:54 To: Hands-Portman, Ian
Hello,
Our client wants us to image proteins using SEM. The protein is dissolved in water and currently stored at -20degrees C. What do you suggest? This is what I'm thinking:
Float a formvar-coated grid on drop of solution or place a drop of solution on a poly-L-lysine cover slip.
Fix grid or cover slip.
Rinse.
Osmicate grid.
Rinse and dry.
Sputter coat.
Thank you very much,
Jaci
Jaclynn Lett Senior Research Technician Research Center for Auditory and Visual Studies Washington University School Of Medicine Saint Louis, Missouri lettj-at-ent.wustl.edu