Welcome to the 19th year of operation of the Microscopy ListServer a free service to the world wide microscopy community, sponsored jointly by your Friendly Neighborhood SysOp and the Microscopy Society of America.
It was productive year for all of us. During 2010, the ListServer delivered 1926 messages to over 3000 subscribers around the world, with minimal hassels (that I know about). For those of you that are statistics junkies this year you generated 460+ Gb of Email traffic and over 5.8 Million Email messages were sent out this year by my tired little server. You don't want to know how much Junk Mail and spam has been filtered out.
The complete Microscopy ListServer Archives for 2010-1993 (~ are on-line at
http://www.microscopy.com.
A couple of final reminders:
If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE your Email address from the listserver. The out-of-office / on-vacation autoreply messages are a real nuisance to posters.
As always if you have questions about suitability of postings or are having problems, feel free to contact me at (zaluzec-at-microscopy.com)
Cheers,
Nestor Your Friendly Neighborhood SysOp
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-----Original Message----- X-from: Confocal Microscopy List [mailto:CONFOCALMICROSCOPY-at-LISTS.UMN.EDU] On Behalf Of Teng-Leong Chew Sent: Saturday, January 01, 2011 3:16 AM To: CONFOCALMICROSCOPY-at-LISTS.UMN.EDU
Thank you, Nestor, and a Happy New Year 2011 to you, too.
My I suggest that all participants at this list server silently give Nestor a big hand for keeping it alive and a valuable resource to everybody (while keeping the spam to a minimum)?
Thanks, Nestor.
Mike --- Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov] Sent: Saturday, January 01, 2011 8:15 AM To: mike.bode-at-resaltatech.com
Happy New Year Colleagues;
Welcome to the 19th year of operation of the Microscopy ListServer a free service to the world wide microscopy community, sponsored jointly by your Friendly Neighborhood SysOp and the Microscopy Society of America.
It was productive year for all of us. During 2010, the ListServer delivered 1926 messages to over 3000 subscribers around the world, with minimal hassels (that I know about). For those of you that are statistics junkies this year you generated 460+ Gb of Email traffic and over 5.8 Million Email messages were sent out this year by my tired little server. You don't want to know how much Junk Mail and spam has been filtered out.
The complete Microscopy ListServer Archives for 2010-1993 (~ are on-line at
http://www.microscopy.com.
A couple of final reminders:
If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE your Email address from the listserver. The out-of-office / on-vacation autoreply messages are a real nuisance to posters.
As always if you have questions about suitability of postings or are having problems, feel free to contact me at (zaluzec-at-microscopy.com)
Cheers,
Nestor Your Friendly Neighborhood SysOp
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I was just working with a MetroCal (I think that's how it's spelled) calibration wafer in an SEM, and I noticed that there is quite a bit of dust on it. Is there a safe and effective way to clean a printed silicon wafer which won't do any damage to it?
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 4, 30 -- From kraftpiano-at-gmail.com Mon Jan 3 14:34:42 2011 4, 30 -- Received: from mail-bw0-f41.google.com (mail-bw0-f41.google.com [209.85.214.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p03KYgBl011394 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 3 Jan 2011 14:34:42 -0600 4, 30 -- Received: by bwz16 with SMTP id 16so16667740bwz.0 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 03 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=domainkey-signature:mime-version:received:received:date:message-id 4, 30 -- :subject:from:to:content-type; 4, 30 -- bh=qHwya0PLKzzf5arg1ByIRcBmNZgGAxILE37Z1eUGd7w=; 4, 30 -- b=ADHwB7MjWoVvu458S9KJNAJWWfwT9aGeIxTuwjwPXaSchhjeiyTlcswrVqOQ+dm3NT 4, 30 -- EsIaLLkiRHtgxc0vnSK2GQo4Z9U7vVlX4zwOfY5qvYXSD/meUYt0CuLdoTn0o8NWuRJi 4, 30 -- hvXzm/kvRE918r4zbu5auzokV1PYoUjMPS3UU= 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 30 -- b=FQnXYcMpueHSMi4XvU2Nlrxdxci+SkGLwREUbM/xnC75MnKqhuMfSBMIbNVeZD5taM 4, 30 -- BM5ZCqxz5DJ1qCWDHWrmxs+fBnDw//SCoTXtIQmCzolpcq3tWcI8Ewe8Yvgh0RQMI4nm 4, 30 -- biBNEKqkU69U/9r+rrXK/wf1q4fVKhRZeyZu4= 4, 30 -- MIME-Version: 1.0 4, 30 -- Received: by 10.204.57.204 with SMTP id d12mr16124775bkh.69.1294086881598; 4, 30 -- Mon, 03 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- Received: by 10.204.60.203 with HTTP; Mon, 3 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- Date: Mon, 3 Jan 2011 15:34:41 -0500 4, 30 -- Message-ID: {AANLkTik+W=37hfEX7EozPoA+VmZioTm2yurMO9XuD-bf-at-mail.gmail.com} 4, 30 -- Subject: Cleaning silicon wafers 4, 30 -- From: Justin Kraft {kraftpiano-at-gmail.com} 4, 30 -- To: microscopy-at-microscopy.com 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Justin, I looked on Metrocal's website and didn't find anything on silicon. Is it perhaps from Geller? Anyway, if you can find the actual manufacturer, there should be cleaning instructions available online.
If not, items on silicon wafers are actually pretty hardy if you don't physically scratch them. A duster, for starters, may take care of the dust. Is it mounted on a stub? Do you know what it's mounted with? Organic solvents (alcohols, acetone) shouldn't hurt the silicon or metallization (usually Al, sometimes Au) followed by a duster. The mounting material is more likely to present a problem than the wafer itself.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 03, 2011 3:38 PM To: kenconverse-at-qualityimages.biz
Greetings everyone, and happy new year!
I was just working with a MetroCal (I think that's how it's spelled) calibration wafer in an SEM, and I noticed that there is quite a bit of dust on it. Is there a safe and effective way to clean a printed silicon wafer which won't do any damage to it?
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pitrone-at-mpi-cbg.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pitrone-at-mpi-cbg.de Name: Peter Pitrone
Organization: Max Planck Institute for Molecular Cell Biology and Genetics
Title-Subject: [Filtered] REMINDER: Microscopist/Imaging-specialist position/s open immediately: MPI-CBG Light Microscopy Facility in Dresden, Germany
Message: Dear friends of microscopy and imaging,
We are very actively searching for 2 microscopists / imaging specialists to join our light microscopy facility team with immediate effect.
The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) in Dresden is seeking motivated Microscopists/Imaging Specialists, to work in its Light Microscopy Facility (LMF).
The LMF is a large core facility enabling different aspects of MPI-CBG researcherís imaging projects including: design of imaging experiments, choice of and training on suitable imaging systems, and image processing, visualisation and analysis. The LMF is part of a network of core imaging facilities on the Dresden Biopolis campus (see https://ifn.mpi-cbg.de.) Light microscopy is one of the key methods of the MPI-CBG. The LMF provides more than 20 advanced imaging systems including laser scanning confocal microscopy, two-photon microscopy, wide-field microscopy, TIRF, SPIM and PALM/STORM. A super resolution structured illumination (SIM) system will be installed in 2011. The LMF team supports approximately 270 users from over 35 countries. More than 40 000 hours of instrument time is booked annually. The working language of the MPI-CBG is English.
Requirements: We are seeking 1-2 candidates who are proactive and service-oriented team players with excellent interpersonal and organizational skills and who enjoy helping people. They should have a first degree (B.Sc.) and preferably also a doctorate degree (Ph.D.) in biology or physics/optics, be fluent in English and be ready to help scientists with both trivial and advanced aspects of light microscopy imaging projects. Experience in basic as well as advanced light microscopy is required and some experience with image analysis would be a strong plus. The successful candidate(s) should be ready to serve in a dynamic international multi-user environment whereby four basic areas of service must be covered: a) scientific support of imaging projects, b) implementation of new imaging technologies, c) maintenance and service of current imaging systems and d) training and teaching light microscopy. Ideally, the candidate(s) should have experience working in an imaging facility.
The positions are available immediately. The initial contract is for 2 years. Compensation will depend on the qualifications and experience of the candidate. The Max Planck Society is committed to employ more disabled persons. The application of disabled persons is strongly encouraged. The Max Planck Society is committed to increase the number of female scientists. Women are particularly encouraged to apply.
For informal inquiries, please contact the leader of the LMF, Dr. Jan Peychl, email: peychl-at-mpi-cbg.de
If you wish to apply for this position, please send your CV, letter of application/motivation and the contact information of three referees to:
Max Planck Institute of Molecular Cell Biology and Genetics Code: 2010-LMF-4100 Pfotenhauerstr. 108 01307 Dresden Germany or by email: 2010-LMF-4100-at-mpi-cbg.de
The University of Wisconsin Eau Claire is seeking applicants for an Analytical Scientist position in the Materials Science Center, an interdisciplinary analytical facility, specializing in materials characterization. This is a full-time professional academic staff position beginning July 1st 2011. Potential applicants may obtain a complete position description and application requirements at http://www.uwec.edu/Matsci/position.html. Women, minorities, individuals with disabilities and veterans are encouraged to apply. Criminal background checks are required prior to employment. Review of completed applications will commence January 14th, 2011 and continue until the position is filled.
The scientist will help manage instrumentation associated with the Materials Science Center, an interdisciplinary analytical facility that incorporates an array of state-of-the-art instrumentation specializing in materials characterization and surface science. Instrumentation within the Center includes a 200 keV Transmission Electron Microscope, Scanning Electron Microscopes with Energy Dispersive X-ray Microanalyzer, a High Resolution Inductively Coupled Plasma - Mass Spectrometer, X-ray Photoelectron Spectrometer, Scanning Tunneling Microscope, 2 X-ray Fluorescence Spectrometers, X-ray Diffractometer, Atomic Force Microscopes, Scanning Auger Nanoprobe and a Micro-FTIR. The scientist will assist with the development of the Electron Microscopy facility, and will be involved in all aspects of technique development, instrument control and maintenance, and other aspects of running a professional laboratory. The scientist will be responsible for instrument maintenance, repair, sample preparation and analysis in other high vacuum applications, such as XRD, XRF, XPS and STM utilized by faculty associated with the Materials Science Center. Precise responsibilities will vary according to research priorities within the Materials Science Center. The scientist is encouraged to participate in collaborative research with faculty, undergraduates and local industry, as well as other scholarly activities. The scientist may be asked to teach some instrument specific courses.
----------------------------------------------------------------------------------- Dr. Doug Dunham Director, Materials Science Center University of Wisconsin-Eau Claire Phillips Hall, Suite 177 105 Garfield Avenue Eau Claire, WI 54702-4004 tel: 715-836-5312 fax: 715-836-3556
==============================Original Headers============================== 9, 32 -- From prvs=498578EF40=DUNHAMDJ-at-uwec.edu Tue Jan 4 11:24:04 2011 9, 32 -- Received: from EX2010-EDGE1.uwec.edu (ex2010-edge1.uwec.edu [137.28.1.209]) 9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p04HO2HI003413 9, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Jan 2011 11:24:03 -0600 9, 32 -- Received: from EX2010-CASHUB1.uwec.edu (137.28.3.34) by EX2010-EDGE1.uwec.edu 9, 32 -- (137.28.1.209) with Microsoft SMTP Server (TLS) id 14.1.270.1; Tue, 4 Jan 9, 32 -- 2011 11:23:49 -0600 9, 32 -- Received: from EX2010-MBX1.uwec.edu ([fe80::d420:b791:b600:11aa]) by 9, 32 -- EX2010-CASHUB1.uwec.edu ([fe80::1ce5:96ef:4fef:7a90%23]) with mapi id 9, 32 -- 14.01.0270.001; Tue, 4 Jan 2011 11:24:01 -0600 9, 32 -- From: "Dunham, Douglas J." {DUNHAMDJ-at-uwec.edu} 9, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 32 -- Subject: Available Position: Analytical Scientist 9, 32 -- Thread-Topic: Available Position: Analytical Scientist 9, 32 -- Thread-Index: AQHLrDQsYRYWsHXo6UGuZpYGK9XJEA== 9, 32 -- Date: Tue, 4 Jan 2011 17:24:00 +0000 9, 32 -- Message-ID: {9802B08AEF25764489DDDF11CB2C9B640CAF270A-at-EX2010-MBX1.uwec.edu} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- x-cr-hashedpuzzle: BcJ+ BhKY C/Yj DGCY ESYW EUdS Elms Fou1 HW3F Hcrn HkL7 9, 32 -- H+1X IDKZ IVZs I1yC 9, 32 -- JBvp;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{98245CD3-83A9-4BC8-9FF8-6E3E71B3275B};ZAB1AG4AaABhAG0AZABqAEAAdQB3AGUAYwAuAGUAZAB1AA==;Tue, 9, 32 -- 04 Jan 2011 17:23:58 9, 32 -- GMT;QQB2AGEAaQBsAGEAYgBsAGUAIABQAG8AcwBpAHQAaQBvAG4AOgAgACAAQQBuAGEAbAB5AHQAaQBjAGEAbAAgAFMAYwBpAGUAbgB0AGkAcwB0AA== 9, 32 -- x-cr-puzzleid: {98245CD3-83A9-4BC8-9FF8-6E3E71B3275B} 9, 32 -- x-originating-ip: [137.28.27.128] 9, 32 -- Content-Type: text/plain; charset="iso-8859-1" 9, 32 -- MIME-Version: 1.0 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p04HO2HI003413 ==============================End of - Headers==============================
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Email: aheath-at-ufl.edu Name: Ann Heatherington
Organization: Dept. of Geological Sciences, University of Florida
Title-Subject: [Filtered] SEM-- Quantomix Wet SEM capsules?
Message: Hello-- Does anyone have any experience with the Wet SEM capsules by Quantomix?
We have a Zeiss EVO MA 10 SEM and every once in a while have someone interested in looking at a wet sample (gels, sediments, etc.) This looked like a possible economical way of gaining wet sample analysis capability, and I would like to hear opinions from someone who has actually used it. The vendor will not supply a customer list.
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Email: kintzjd-at-rocketmail.com Name: Joe Kintz
Organization: Stress Engineering Services, Inc.
Title-Subject: [Filtered] Repair or replace EDS detector?
Message: Our EDAX-brand Sapphire Si(Li) EDS detector probably has a crack or hole in its super ultra thin window and will probably have to be sent to EDAX for repair. Is this a good time to consider upgrading to a silicon drift detector? Using liquid nitrogen is not a problem for us but, if we can improve EDS performance at a reasonable price compared to repairing our existing Si(Li) detector, this would seem to make sense. We're also using EDAX's EBSD system, so we'd like to continue using their Genesis EDS software.
If this would be a good time to upgrade, what is the concensus about the silicon drift detectors (SDD)? Are they better across the board than the Si(Li) detectors, or would we be giving up some aspect of performance? Who makes the best SDD's that will work with EDAX EDS systems?
I would agree with Ken on the use of a compressed gas as a first attempt at cleaning your wafer, but I would actually avoid those tetrafluoroethane dusters. It's been my experience that they can leave a film on the surface of the wafer. If you have a spare piece of polished silicon wafer hanging around you could test the duster on it to see if it leaves a film. I prefer using high purity dry nitrogen from a gas cylinder, but I have also used the compressed CO2 dusters you can find at office supply stores with good results.
Since it looks like this wafer is etched silicon, you should be safe using a solvent if there is anything stuck on it, but I would stress that you should contact the manufacturer (Metroboost*) to make sure that they don't have some kind of a coating on it or something that would be damaged by a particular solvent. A good general choice to start would actually be deionized water, since the etch process to make the wafer probably employed that anyway. The procedure that we used during my semiconductor courses was to rinse the chip or wafer with DI water and then blow-dry it with dry nitrogen. It helps if you hold the wafer near vertical with one end resting on a lint-free paper towel and then blow the residual solvent towards the towel starting from the top of the wafer and working your way down. This generally leaves a clean, lint free, surface.
Hope that helps!
Mike Anderson
*Comments made in this correspondence represent the opinion of the author and should not be construed as endorsement or recommendation by NIST. Likewise, any mention of commercial products does not imply recommendation or endorsement by NIST.
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: Monday, January 03, 2011 5:51 PM To: Anderson, Michael
Justin, I looked on Metrocal's website and didn't find anything on silicon. Is it perhaps from Geller? Anyway, if you can find the actual manufacturer, there should be cleaning instructions available online.
If not, items on silicon wafers are actually pretty hardy if you don't physically scratch them. A duster, for starters, may take care of the dust. Is it mounted on a stub? Do you know what it's mounted with? Organic solvents (alcohols, acetone) shouldn't hurt the silicon or metallization (usually Al, sometimes Au) followed by a duster. The mounting material is more likely to present a problem than the wafer itself.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 03, 2011 3:38 PM To: kenconverse-at-qualityimages.biz
Greetings everyone, and happy new year!
I was just working with a MetroCal (I think that's how it's spelled) calibration wafer in an SEM, and I noticed that there is quite a bit of dust on it. Is there a safe and effective way to clean a printed silicon wafer which won't do any damage to it?
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
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==============================Original Headers============================== 18, 28 -- From kenconverse-at-qualityimages.biz Mon Jan 3 16:41:56 2011 18, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p03Mfuvf031739 18, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Jan 2011 16:41:56 -0600 18, 28 -- X-Authority-Analysis: v=1.1 cv=uESSSoDEku2quKX/oFXS2Smn5+55LTFcWFr5T5T8nFs= c=1 sm=0 a=EPkJnWdmquUA:10 a=kj9zAlcOel0A:10 a=o/BqynnMLxMJiB4/byRFHg==:17 a=pGLkceISAAAA:8 a=Zx37jsudAAAA:8 a=1XWaLZrsAAAA:8 a=ncwpZeBwXMnrfOMQ3bQA:9 a=US01wcXEiGvzWnChvZQA:7 a=1H2jpXTeLfj6DHcIsEfKllJOVbMA:4 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=fM1NKZWFy18A:10 a=RyjLG6jaqdoA:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=o/BqynnMLxMJiB4/byRFHg==:117 18, 28 -- X-Cloudmark-Score: 0 18, 28 -- X-Originating-IP: 72.227.97.248 18, 28 -- Received: from [72.227.97.248] ([72.227.97.248:4582] helo=Ken) 18, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 18, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 18, 28 -- id 83/2A-07087-4B0522D4; Mon, 03 Jan 2011 22:41:56 +0000 18, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 18, 28 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" {microscopy-at-microscopy.com} 18, 28 -- Subject: RE: [Microscopy] Cleaning silicon wafers 18, 28 -- Date: Mon, 3 Jan 2011 17:41:48 -0500 18, 28 -- Message-ID: {9F9035F8BFEB4E3FB7E478F4EC892D5C-at-Ken} 18, 28 -- MIME-Version: 1.0 18, 28 -- Content-Type: text/plain; 18, 28 -- charset="us-ascii" 18, 28 -- X-Priority: 3 (Normal) 18, 28 -- X-MSMail-Priority: Normal 18, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 18, 28 -- In-Reply-To: {201101032038.p03Kc1CM015029-at-ns.microscopy.com} 18, 28 -- Importance: Normal 18, 28 -- Thread-Index: Acurhhz1BXcIliXoQuu6YgNFf4AoAAAECPwg 18, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 18, 28 -- Content-Transfer-Encoding: 8bit 18, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p03Mfuvf031739 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 28 -- From michael.anderson-at-nist.gov Wed Jan 5 09:17:09 2011 30, 28 -- Received: from smtp.nist.gov (rimp1.nist.gov [129.6.16.226]) 30, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05FH8nR024260 30, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 09:17:09 -0600 30, 28 -- Received: from WSXGHUB2.xchange.nist.gov (wsxghub2.nist.gov [129.6.18.19]) 30, 28 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id p05FH4c9030458 30, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 10:17:04 -0500 30, 28 -- Received: from MBCLUSTER.xchange.nist.gov ([fe80::d479:3188:aec0:cb66]) by 30, 28 -- WSXGHUB2.xchange.nist.gov ([129.6.18.19]) with mapi; Wed, 5 Jan 2011 10:16:53 30, 28 -- -0500 30, 28 -- From: "Anderson, Michael" {michael.anderson-at-nist.gov} 30, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 30, 28 -- Date: Wed, 5 Jan 2011 10:16:59 -0500 30, 28 -- Subject: RE: [Microscopy] RE: Cleaning silicon wafers 30, 28 -- Thread-Topic: [Microscopy] RE: Cleaning silicon wafers 30, 28 -- Thread-Index: AcurmKiv9OavxWSyRr27x/LZnO5vWABTZMMg 30, 28 -- Message-ID: {7E80450DE7474E4881E405C742AC0DEA11DA4A75D2-at-MBCLUSTER.xchange.nist.gov} 30, 28 -- References: {201101032250.p03Mok37014851-at-ns.microscopy.com} 30, 28 -- In-Reply-To: {201101032250.p03Mok37014851-at-ns.microscopy.com} 30, 28 -- Accept-Language: en-US 30, 28 -- Content-Language: en-US 30, 28 -- X-MS-Has-Attach: 30, 28 -- X-MS-TNEF-Correlator: 30, 28 -- acceptlanguage: en-US 30, 28 -- Content-Type: text/plain; charset="us-ascii" 30, 28 -- MIME-Version: 1.0 30, 28 -- X-NIST-MailScanner: Found to be clean 30, 28 -- X-NIST-MailScanner-From: michael.anderson-at-nist.gov ==============================End of - Headers==============================
--- On Wed, 1/5/11, michael.anderson-at-nist.gov {michael.anderson-at-nist.gov} wrote:
} From: michael.anderson-at-nist.gov {michael.anderson-at-nist.gov} } Subject: [Microscopy] Cleaning silicon wafers } To: mcintoshgreece-at-yahoo.com } Date: Wednesday, January 5, 2011, 10:23 AM } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin, } } I would agree with Ken on the use of a compressed gas as a } first attempt at cleaning your wafer, but I would actually } avoid those tetrafluoroethane dusters. It's been my } experience that they can leave a film on the surface of the } wafer. If you have a spare piece of polished silicon } wafer hanging around you could test the duster on it to see } if it leaves a film. I prefer using high purity dry } nitrogen from a gas cylinder, but I have also used the } compressed CO2 dusters you can find at office supply stores } with good results. } } Since it looks like this wafer is etched silicon, you } should be safe using a solvent if there is anything stuck on } it, but I would stress that you should contact the } manufacturer (Metroboost*) to make sure that they don't have } some kind of a coating on it or something that would be } damaged by a particular solvent. A good general choice to } start would actually be deionized water, since the etch } process to make the wafer probably employed that } anyway. The procedure that we used during my } semiconductor courses was to rinse the chip or wafer with DI } water and then blow-dry it with dry nitrogen. It helps } if you hold the wafer near vertical with one end resting on } a lint-free paper towel and then blow the residual solvent } towards the towel starting from the top of the wafer and } working your way down. This generally leaves a clean, } lint free, surface. } } Hope that helps! } } Mike Anderson
} Justin, } I looked on Metrocal's website and didn't find anything on } silicon. Is it } perhaps from Geller? Anyway, if you can find the } actual manufacturer, there } should be cleaning instructions available online. } } If not, items on silicon wafers are actually pretty hardy } if you don't } physically scratch them. A duster, for starters, may } take care of the dust. } Is it mounted on a stub? Do you know what it's } mounted with? Organic } solvents (alcohols, acetone) shouldn't hurt the silicon or } metallization } (usually Al, sometimes Au) followed by a duster. The } mounting material is } more likely to present a problem than the wafer itself. } } Ken Converse } owner } } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz }
} Greetings everyone, and happy new year! } } I was just working with a MetroCal (I think that's how it's } spelled) } calibration wafer in an SEM, and I noticed that there is } quite a bit } of dust on it. Is there a safe and effective way to } clean a printed } silicon wafer which won't do any damage to it? } } --Justin A. Kraft }
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Check this product as well: http://www.vatran.com/dryice.html
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==============================Original Headers============================== 9, 21 -- From SHISHKOV-at-HELIX.MGH.HARVARD.EDU Wed Jan 5 14:03:24 2011 9, 21 -- Received: from phsmgmx12.partners.org (phsmgmx12.partners.org [155.52.251.71]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05K3OBC005841 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 14:03:24 -0600 9, 21 -- X-IronPort-Anti-Spam-Filtered: true 9, 21 -- X-IronPort-Anti-Spam-Result: Aq8RAEVdJE2Et4ImgWdsb2JhbACWB4YRiAMVAQEWIiS5MoYDAoMMgj4EhGiGIoMfBg 9, 21 -- Received: from phsxcon5.partners.org ([132.183.130.38]) 9, 21 -- by phsmgmx12-out.partners.org with ESMTP; 05 Jan 2011 15:03:24 -0500 9, 21 -- Received: from [132.183.44.148] ([132.183.44.148]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.4675); 9, 21 -- Wed, 5 Jan 2011 15:03:24 -0500 9, 21 -- Message-ID: {4D24CE8B.2050900-at-helix.mgh.harvard.edu} 9, 21 -- Date: Wed, 05 Jan 2011 15:03:23 -0500 9, 21 -- From: Milen Shishkov {shishkov-at-HELIX.MGH.HARVARD.EDU} 9, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.8) Gecko/20100802 Lightning/1.0b2 Thunderbird/3.1.2 9, 21 -- MIME-Version: 1.0 9, 21 -- To: microscopy-at-microscopy.com 9, 21 -- Subject: RE: Cleaning silicon wafers 9, 21 -- X-OriginalArrivalTime: 05 Jan 2011 20:03:24.0119 (UTC) FILETIME=[9BDDF670:01CBAD13] 9, 21 -- Content-Type: text/plain; charset="iso-8859-1"; format="flowed" 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p05K3OBC005841 ==============================End of - Headers==============================
We have a CPD from tousimis (815 AutoSamDri, Series A)
Pricey, but automatic and based on PhysChem. As tousimis makes big ones for complete mems wafers, plain silicon chips (i.e. 5 x 5mm) are not a problem. Biggest problem is, once retrieved in open lab, how does one protect the chip from instant contamination. My followup would be plasma cleaning to clear off any carbon deposited between CPD and final use.
If all were done in clean room or gloved environment, then CPD - despite cost - may be the way to go both before and after processing the wafer). I would strongly recommend enthusiastic consideration.
Cheers,
Fred Monson
P.S. I'm a biologist first, so I am poking my head into an other's place. Thus, I realize the value of advice may appear depreciated by background. Howsoever, please consider the fact that since we messy organic microscopic investigators spend our lives looking at artifact and gathering together to grant each other various levels of group reality for each and every microscopic finding, we are also those who strive most eagerly for the purity of mere 3D problems. To save an ultrastructural projection on the surface of a cell, there is NOTHING like perfect CPD.
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: shishkov-at-HELIX.MGH.HARVARD.EDU [mailto:shishkov-at-HELIX.MGH.HARVARD.EDU] Sent: Wednesday, January 05, 2011 3:11 PM To: Monson, Frederick
Check this product as well: http://www.vatran.com/dryice.html
-- Milen Shishkov, Ph.D. Wellman Center for Photomedicine Massachusetts General Hospital Harvard Medical School
Address: MGH BAR 712 50 Blossom St. Boston, MA 02114 Phone: (617) 726 1589 Fax: (617) 643 9208
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==============================Original Headers============================== 9, 21 -- From SHISHKOV-at-HELIX.MGH.HARVARD.EDU Wed Jan 5 14:03:24 2011 9, 21 -- Received: from phsmgmx12.partners.org (phsmgmx12.partners.org [155.52.251.71]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05K3OBC005841 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 14:03:24 -0600 9, 21 -- X-IronPort-Anti-Spam-Filtered: true 9, 21 -- X-IronPort-Anti-Spam-Result: Aq8RAEVdJE2Et4ImgWdsb2JhbACWB4YRiAMVAQEWIiS5MoYDAoMMgj4EhGiGIoMfBg 9, 21 -- Received: from phsxcon5.partners.org ([132.183.130.38]) 9, 21 -- by phsmgmx12-out.partners.org with ESMTP; 05 Jan 2011 15:03:24 -0500 9, 21 -- Received: from [132.183.44.148] ([132.183.44.148]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.4675); 9, 21 -- Wed, 5 Jan 2011 15:03:24 -0500 9, 21 -- Message-ID: {4D24CE8B.2050900-at-helix.mgh.harvard.edu} 9, 21 -- Date: Wed, 05 Jan 2011 15:03:23 -0500 9, 21 -- From: Milen Shishkov {shishkov-at-HELIX.MGH.HARVARD.EDU} 9, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.8) Gecko/20100802 Lightning/1.0b2 Thunderbird/3.1.2 9, 21 -- MIME-Version: 1.0 9, 21 -- To: microscopy-at-microscopy.com 9, 21 -- Subject: RE: Cleaning silicon wafers 9, 21 -- X-OriginalArrivalTime: 05 Jan 2011 20:03:24.0119 (UTC) FILETIME=[9BDDF670:01CBAD13] 9, 21 -- Content-Type: text/plain; charset="iso-8859-1"; format="flowed" 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p05K3OBC005841 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 29 -- From FMonson-at-wcupa.edu Wed Jan 5 16:51:39 2011 27, 29 -- Received: from WCU-EX-ET1.PASSHE.LCL (wcu-ex-et1-auth.wcupa.edu [144.26.0.82]) 27, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05Mpd4W028986 27, 29 -- for {microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 16:51:39 -0600 27, 29 -- Received: from WCU-XCH-04.PASSHE.LCL (10.32.4.191) by WCU-EX-ET1.PASSHE.LCL 27, 29 -- (10.32.0.62) with Microsoft SMTP Server (TLS) id 8.2.213.0; Wed, 5 Jan 2011 27, 29 -- 17:51:38 -0500 27, 29 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 27, 29 -- WCU-XCH-04.PASSHE.LCL ([fe80::58fd:1d03:b92e:36bd%16]) with mapi id 27, 29 -- 14.01.0255.000; Wed, 5 Jan 2011 17:51:37 -0500 27, 29 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 27, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 27, 29 -- CC: "shishkov-at-HELIX.MGH.HARVARD.EDU" {shishkov-at-HELIX.MGH.HARVARD.EDU} 27, 29 -- Subject: RE: [Microscopy] RE: Cleaning silicon wafers 27, 29 -- Thread-Topic: [Microscopy] RE: Cleaning silicon wafers 27, 29 -- Thread-Index: AQHLrRSbqF/vmwR8D0iqF4FGHovSUJPC97/g 27, 29 -- Date: Wed, 5 Jan 2011 22:51:37 +0000 27, 29 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30CAC2A-at-WCU-XCH-03.PASSHE.LCL} 27, 29 -- References: {201101052010.p05KAVoI019910-at-ns.microscopy.com} 27, 29 -- In-Reply-To: {201101052010.p05KAVoI019910-at-ns.microscopy.com} 27, 29 -- Accept-Language: en-US 27, 29 -- Content-Language: en-US 27, 29 -- X-MS-Has-Attach: 27, 29 -- X-MS-TNEF-Correlator: 27, 29 -- x-originating-ip: [10.28.57.79] 27, 29 -- Content-Type: text/plain; charset="us-ascii" 27, 29 -- MIME-Version: 1.0 27, 29 -- Content-Transfer-Encoding: 8bit 27, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p05Mpd4W028986 ==============================End of - Headers==============================
I'll just echo what Ken Converse and Mike Anderson have to say and add my 2 cents' worth. I work with Si wafers (patterned and unpatterned) everyday. I also have an SEM standard similar to the one you are using. I usually clean mine (after rapping the knuckles of the person who got it dirty) first with dry N2. If you have some stubborn dust particles, a short sonication in warm DI or distilled water should do it. One of those little brushes that photographers used to use to get dust off negatives would be good also, since they neutralize the static charges that make for some tenacious particles. I have deionizers all over the place, so I use those. After all the effort you put into cleaning the standard, store it in as dust-free an atmosphere and container as you have available. I store mine in the metal box it came in, in a dry nitrogen cabinet.
If some ham-handed user has stuck their finger to it, that's more difficult. CO2 snow is a wizard for removing organic contaminants such as finger oils but not everybody has access to a unit. In this case (if the stub attachment will stand it) I would try reagent-grade (or fab-grade) acetone, using it in the manner Mike suggests. I would follow this with some reagent- or fab-grade methanol as sometimes acetone can leave a residue.
On 1/5/11 9:17 AM, michael.anderson-at-nist.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin, } } I would agree with Ken on the use of a compressed gas as a first attempt at cleaning your wafer, but I would actually avoid those tetrafluoroethane dusters. It's been my experience that they can leave a film on the surface of the wafer. If you have a spare piece of polished silicon wafer hanging around you could test the duster on it to see if it leaves a film. I prefer using high purity dry nitrogen from a gas cylinder, but I have also used the compressed CO2 dusters you can find at office supply stores with good results. } } Since it looks like this wafer is etched silicon, you should be safe using a solvent if there is anything stuck on it, but I would stress that you should contact the manufacturer (Metroboost*) to make sure that they don't have some kind of a coating on it or something that would be damaged by a particular solvent. A good general choice to start would actually be deionized water, since the etch process to make the wafer probably employed that anyway. The procedure that we used during my semiconductor courses was to rinse the chip or wafer with DI water and then blow-dry it with dry nitrogen. It helps if you hold the wafer near vertical with one end resting on a lint-free paper towel and then blow the residual solvent towards the towel starting from the top of the wafer and working your way down. This generally leaves a clean, lint free, surface. } } Hope that helps! } } Mike Anderson } } *Comments made in this correspondence represent the opinion of the author and should not be construed as endorsement or recommendation by NIST. Likewise, any mention of commercial products does not imply recommendation or endorsement by NIST. } } } -----Original Message----- } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } Sent: Monday, January 03, 2011 5:51 PM } To: Anderson, Michael } Subject: [Microscopy] RE: Cleaning silicon wafers } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin, } I looked on Metrocal's website and didn't find anything on silicon. Is it } perhaps from Geller? Anyway, if you can find the actual manufacturer, there } should be cleaning instructions available online. } } If not, items on silicon wafers are actually pretty hardy if you don't } physically scratch them. A duster, for starters, may take care of the dust. } Is it mounted on a stub? Do you know what it's mounted with? Organic } solvents (alcohols, acetone) shouldn't hurt the silicon or metallization } (usually Al, sometimes Au) followed by a duster. The mounting material is } more likely to present a problem than the wafer itself. } } Ken Converse } owner } } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } Sent: Monday, January 03, 2011 3:38 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] Cleaning silicon wafers } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Greetings everyone, and happy new year! } } I was just working with a MetroCal (I think that's how it's spelled) } calibration wafer in an SEM, and I noticed that there is quite a bit } of dust on it. Is there a safe and effective way to clean a printed } silicon wafer which won't do any damage to it? } } --Justin A. Kraft }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 214-567-0360 SC Packaging FA Texas Instruments, Inc. 13536 N. Central Expressway MS 940 Dallas, TX 75243 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 5, 24 -- From r-holdford-at-ti.com Wed Jan 5 17:49:40 2011 5, 24 -- Received: from arroyo.ext.ti.com (arroyo.ext.ti.com [192.94.94.40]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05Nne0a013376 5, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 17:49:40 -0600 5, 24 -- Received: from dlep35.itg.ti.com ([157.170.170.118]) 5, 24 -- by arroyo.ext.ti.com (8.13.7/8.13.7) with ESMTP id p05NncwQ026078 5, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 24 -- Wed, 5 Jan 2011 17:49:38 -0600 5, 24 -- Received: from [156.117.194.82] (localhost [127.0.0.1]) 5, 24 -- by dlep35.itg.ti.com (8.13.7/8.13.7) with ESMTP id p05NncTA024701; 5, 24 -- Wed, 5 Jan 2011 17:49:38 -0600 (CST) 5, 24 -- Message-ID: {4D250392.4060002-at-ti.com} 5, 24 -- Date: Wed, 05 Jan 2011 17:49:38 -0600 5, 24 -- From: Becky Holdford {r-holdford-at-ti.com} 5, 24 -- Organization: SC Packaging MPI Team 5, 24 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 5, 24 -- MIME-Version: 1.0 5, 24 -- To: Justin Kraft {kraftpiano-at-gmail.com} , 5, 24 -- MSA Listserver {Microscopy-at-microscopy.com} 5, 24 -- Subject: Re: [Microscopy] Cleaning silicon wafers 5, 24 -- References: {201101051517.p05FHXqN024496-at-ns.microscopy.com} 5, 24 -- In-Reply-To: {201101051517.p05FHXqN024496-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
No special unit is needed for CO2 snow, just a tank of Clean! CO2. Either remove any hose, or attach the tank hose to something weighty - any block of metal with a threaded hole into which the hose can be screwed, or the like. Something to hold the hose firmly so it doesn't whip around. Turn on the tank, and the expanding CO2 makes snow. (I used to do this into a pillow case for sending samples from the Arctic.) This worked with both regular and siphon CO2 tanks. Now, I use the exhaust outlet (not "Vent") of our Polaron bomb CPD if I need snow. (Because of the weightiness of the CPD.) I suggest food grade CO2, but an absolutely dry grade may be needed for your application.
Phil
} I'll just echo what Ken Converse and Mike Anderson have to say } and add my 2 cents' worth. I work with Si wafers (patterned and } unpatterned) everyday. I also have an SEM standard similar to } the one you are using. I usually clean mine (after rapping the } knuckles of the person who got it dirty) first with dry N2. If } you have some stubborn dust particles, a short sonication in warm } DI or distilled water should do it. One of those little brushes } that photographers used to use to get dust off negatives would be } good also, since they neutralize the static charges that make for } some tenacious particles. I have deionizers all over the place, } so I use those. After all the effort you put into cleaning the } standard, store it in as dust-free an atmosphere and container as } you have available. I store mine in the metal box it came in, in } a dry nitrogen cabinet. } } If some ham-handed user has stuck their finger to it, that's more } difficult. CO2 snow is a wizard for removing organic } contaminants such as finger oils but not everybody has access to } a unit. In this case (if the stub attachment will stand it) I } would try reagent-grade (or fab-grade) acetone, using it in the } manner Mike suggests. I would follow this with some reagent- or } fab-grade methanol as sometimes acetone can leave a residue. } } On 1/5/11 9:17 AM, michael.anderson-at-nist.gov wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Justin, } } } } I would agree with Ken on the use of a compressed gas as a first } } attempt at cleaning your wafer, but I would actually avoid those } } tetrafluoroethane dusters. It's been my experience that they can } } leave a film on the surface of the wafer. If you have a spare } } piece of polished silicon wafer hanging around you could test the } } duster on it to see if it leaves a film. I prefer using high } } purity dry nitrogen from a gas cylinder, but I have also used the } } compressed CO2 dusters you can find at office supply stores with } } good results. } } } } Since it looks like this wafer is etched silicon, you should be } } safe using a solvent if there is anything stuck on it, but I would } } stress that you should contact the manufacturer (Metroboost*) to } } make sure that they don't have some kind of a coating on it or } } something that would be damaged by a particular solvent. A good } } general choice to start would actually be deionized water, since } } the etch process to make the wafer probably employed that anyway. } } The procedure that we used during my semiconductor courses was to } } rinse the chip or wafer with DI water and then blow-dry it with dry } } nitrogen. It helps if you hold the wafer near vertical with one } } end resting on a lint-free paper towel and then blow the residual } } solvent towards the towel starting from the top of the wafer and } } working your way down. This generally leaves a clean, lint free, } } surface. } } } } Hope that helps! } } } } Mike Anderson } } } } *Comments made in this correspondence represent the opinion of the } } author and should not be construed as endorsement or recommendation } } by NIST. Likewise, any mention of commercial products does not } } imply recommendation or endorsement by NIST. } } } } } } -----Original Message----- } } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } } Sent: Monday, January 03, 2011 5:51 PM } } To: Anderson, Michael } } Subject: [Microscopy] RE: Cleaning silicon wafers } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Justin, } } I looked on Metrocal's website and didn't find anything on silicon. Is it } } perhaps from Geller? Anyway, if you can find the actual manufacturer, there } } should be cleaning instructions available online. } } } } If not, items on silicon wafers are actually pretty hardy if you don't } } physically scratch them. A duster, for starters, may take care of the dust. } } Is it mounted on a stub? Do you know what it's mounted with? Organic } } solvents (alcohols, acetone) shouldn't hurt the silicon or metallization } } (usually Al, sometimes Au) followed by a duster. The mounting material is } } more likely to present a problem than the wafer itself. } } } } Ken Converse } } owner } } } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes } } Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } Fax 207-647-2688 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } -----Original Message----- } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } } Sent: Monday, January 03, 2011 3:38 PM } } To: kenconverse-at-qualityimages.biz } } Subject: [Microscopy] Cleaning silicon wafers
} } Greetings everyone, and happy new year! } } } } I was just working with a MetroCal (I think that's how it's spelled) } } calibration wafer in an SEM, and I noticed that there is quite a bit } } of dust on it. Is there a safe and effective way to clean a printed } } silicon wafer which won't do any damage to it? } } } } --Justin A. Kraft } } } } -- } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Becky Holdford (r-holdford-at-ti.com) } 214-567-0360 } SC Packaging FA } Texas Instruments, Inc. } 13536 N. Central Expressway MS 940 } Dallas, TX 75243 } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 25 -- From oshel1pe-at-cmich.edu Thu Jan 6 07:09:46 2011 5, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p06D9j38028551 5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Jan 2011 07:09:46 -0600 5, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 25 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p06D9Zuu031961 5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Jan 2011 08:09:45 -0500 5, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 5, 25 -- Thu, 6 Jan 2011 08:09:43 -0500 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {a06240801c94b6e1e6a5c-at-[141.209.160.249]} 5, 25 -- In-Reply-To: {201101052352.p05NqhEg018637-at-ns.microscopy.com} 5, 25 -- References: {201101052352.p05NqhEg018637-at-ns.microscopy.com} 5, 25 -- Date: Thu, 6 Jan 2011 08:09:39 -0500 5, 25 -- To: Microscopy-at-microscopy.com 5, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 25 -- Subject: [Microscopy] Re: Cleaning silicon wafers 5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 25 -- X-OriginalArrivalTime: 06 Jan 2011 13:09:43.0431 (UTC) FILETIME=[FBFF4970:01CBADA2] 5, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 25 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 5, 25 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 25 -- X-CanItPRO-Stream: default 5, 25 -- X-Canit-Stats-ID: 02DQd9J1n - 93d678b9fa6a - 20110106 5, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Phil: thanks bunches for this cool (pun intended) tip!
On 1/6/11 7:09 AM, oshel1pe-at-cmich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } No special unit is needed for CO2 snow, just a tank of Clean! CO2. } Either remove any hose, or attach the tank hose to something weighty } - any block of metal with a threaded hole into which the hose can be } screwed, or the like. Something to hold the hose firmly so it doesn't } whip around. Turn on the tank, and the expanding CO2 makes snow. (I } used to do this into a pillow case for sending samples from the } Arctic.) This worked with both regular and siphon CO2 tanks. } Now, I use the exhaust outlet (not "Vent") of our Polaron bomb CPD if } I need snow. (Because of the weightiness of the CPD.) } I suggest food grade CO2, but an absolutely dry grade may be needed } for your application. } } Phil } } } I'll just echo what Ken Converse and Mike Anderson have to say } } and add my 2 cents' worth. I work with Si wafers (patterned and } } unpatterned) everyday. I also have an SEM standard similar to } } the one you are using. I usually clean mine (after rapping the } } knuckles of the person who got it dirty) first with dry N2. If } } you have some stubborn dust particles, a short sonication in warm } } DI or distilled water should do it. One of those little brushes } } that photographers used to use to get dust off negatives would be } } good also, since they neutralize the static charges that make for } } some tenacious particles. I have deionizers all over the place, } } so I use those. After all the effort you put into cleaning the } } standard, store it in as dust-free an atmosphere and container as } } you have available. I store mine in the metal box it came in, in } } a dry nitrogen cabinet. } } } } If some ham-handed user has stuck their finger to it, that's more } } difficult. CO2 snow is a wizard for removing organic } } contaminants such as finger oils but not everybody has access to } } a unit. In this case (if the stub attachment will stand it) I } } would try reagent-grade (or fab-grade) acetone, using it in the } } manner Mike suggests. I would follow this with some reagent- or } } fab-grade methanol as sometimes acetone can leave a residue. } } } } On 1/5/11 9:17 AM, michael.anderson-at-nist.gov wrote: } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Justin, } } } } } } I would agree with Ken on the use of a compressed gas as a first } } } attempt at cleaning your wafer, but I would actually avoid those } } } tetrafluoroethane dusters. It's been my experience that they can } } } leave a film on the surface of the wafer. If you have a spare } } } piece of polished silicon wafer hanging around you could test the } } } duster on it to see if it leaves a film. I prefer using high } } } purity dry nitrogen from a gas cylinder, but I have also used the } } } compressed CO2 dusters you can find at office supply stores with } } } good results. } } } } } } Since it looks like this wafer is etched silicon, you should be } } } safe using a solvent if there is anything stuck on it, but I would } } } stress that you should contact the manufacturer (Metroboost*) to } } } make sure that they don't have some kind of a coating on it or } } } something that would be damaged by a particular solvent. A good } } } general choice to start would actually be deionized water, since } } } the etch process to make the wafer probably employed that anyway. } } } The procedure that we used during my semiconductor courses was to } } } rinse the chip or wafer with DI water and then blow-dry it with dry } } } nitrogen. It helps if you hold the wafer near vertical with one } } } end resting on a lint-free paper towel and then blow the residual } } } solvent towards the towel starting from the top of the wafer and } } } working your way down. This generally leaves a clean, lint free, } } } surface. } } } } } } Hope that helps! } } } } } } Mike Anderson } } } } } } *Comments made in this correspondence represent the opinion of the } } } author and should not be construed as endorsement or recommendation } } } by NIST. Likewise, any mention of commercial products does not } } } imply recommendation or endorsement by NIST. } } } } } } } } } -----Original Message----- } } } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } } } Sent: Monday, January 03, 2011 5:51 PM } } } To: Anderson, Michael } } } Subject: [Microscopy] RE: Cleaning silicon wafers } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Justin, } } } I looked on Metrocal's website and didn't find anything on silicon. Is it } } } perhaps from Geller? Anyway, if you can find the actual manufacturer, there } } } should be cleaning instructions available online. } } } } } } If not, items on silicon wafers are actually pretty hardy if you don't } } } physically scratch them. A duster, for starters, may take care of the dust. } } } Is it mounted on a stub? Do you know what it's mounted with? Organic } } } solvents (alcohols, acetone) shouldn't hurt the silicon or metallization } } } (usually Al, sometimes Au) followed by a duster. The mounting material is } } } more likely to present a problem than the wafer itself. } } } } } } Ken Converse } } } owner } } } } } } } } } QUALITY IMAGES } } } Servicing Scanning Electron Microscopes } } } Since 1981 } } } 474 So. Bridgton Rd. } } } Bridgton, ME 04009 } } } 207-647-4348 } } } Fax 207-647-2688 } } } kenconverse-at-qualityimages.biz } } } qualityimages.biz } } } } } } } } } -----Original Message----- } } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } } } Sent: Monday, January 03, 2011 3:38 PM } } } To: kenconverse-at-qualityimages.biz } } } Subject: [Microscopy] Cleaning silicon wafers } } } Greetings everyone, and happy new year! } } } } } } I was just working with a MetroCal (I think that's how it's spelled) } } } calibration wafer in an SEM, and I noticed that there is quite a bit } } } of dust on it. Is there a safe and effective way to clean a printed } } } silicon wafer which won't do any damage to it? } } } } } } --Justin A. Kraft } } } } } -- } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } Becky Holdford (r-holdford-at-ti.com) } } 214-567-0360 } } SC Packaging FA } } Texas Instruments, Inc. } } 13536 N. Central Expressway MS 940 } } Dallas, TX 75243 } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 214-567-0360 SC Packaging FA Texas Instruments, Inc. 13536 N. Central Expressway MS 940 Dallas, TX 75243 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 4, 23 -- From r-holdford-at-ti.com Thu Jan 6 14:10:41 2011 4, 23 -- Received: from arroyo.ext.ti.com (arroyo.ext.ti.com [192.94.94.40]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p06KAfSB026291 4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Jan 2011 14:10:41 -0600 4, 23 -- Received: from dlep36.itg.ti.com ([157.170.170.91]) 4, 23 -- by arroyo.ext.ti.com (8.13.7/8.13.7) with ESMTP id p06KAcrD012870 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 23 -- Thu, 6 Jan 2011 14:10:38 -0600 4, 23 -- Received: from [156.117.194.82] (localhost [127.0.0.1]) 4, 23 -- by dlep36.itg.ti.com (8.13.8/8.13.8) with ESMTP id p06KAbqF025335; 4, 23 -- Thu, 6 Jan 2011 14:10:38 -0600 (CST) 4, 23 -- Message-ID: {4D2621BC.3050105-at-ti.com} 4, 23 -- Date: Thu, 06 Jan 2011 14:10:36 -0600 4, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 23 -- Organization: SC Packaging MPI Team 4, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 4, 23 -- MIME-Version: 1.0 4, 23 -- To: oshel1pe-at-cmich.edu, MSA Listserver {Microscopy-at-microscopy.com} 4, 23 -- Subject: Re: [Microscopy] Re: Cleaning silicon wafers 4, 23 -- References: {201101061309.p06D9uge028766-at-ns.microscopy.com} 4, 23 -- In-Reply-To: {201101061309.p06D9uge028766-at-ns.microscopy.com} 4, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The EM Lab at Duke Hospital has gone digital so we have a couple of darkroom goodies to give away.
Of course, you must pay for the shipping and handling but think of all the money you'll save!
The items are:
Ilford 2150 RC print processor and 3 boxes which contain one jug of Ilford developer and one jug of Ilford fixer each. This baby is in fantastic condition and I even cleaned it so it is all sparkly and ready to move into your darkroom. You don't even have to kick the tires, plus we even have the original owners manual.
A lot of film holders for Zeiss, JEOL and maybe Hitachi. Some of these holders are for the more vintage TEM models.
If you have any questions, please e-mail Andy Kloiber at andykloiber-at-yahoo.com.
I will be on vacation next week so Andy can help you.
First come, first served and all that jazz.
More items to come as we slowly clean the darkroom out.
Enjoy your weekend,
Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#M251 Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 13, 33 -- From patpxs-at-gmail.com Thu Jan 6 16:23:45 2011 13, 33 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 13, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p06MNiSQ013108 13, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Jan 2011 16:23:44 -0600 13, 33 -- Received: by iwn40 with SMTP id 40so18633574iwn.0 13, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 06 Jan 2011 14:23:44 -0800 (PST) 13, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 33 -- d=gmail.com; s=gamma; 13, 33 -- h=domainkey-signature:mime-version:received:received:date:message-id 13, 33 -- :subject:from:to:content-type:content-transfer-encoding; 13, 33 -- bh=pGgL4fYyFzEgk2LR7a1gZ2znefAyFyPa1kOui8qX9lQ=; 13, 33 -- b=qDyRQgj/wc899NsbteFPLf4wJbAlP2doW+FxiT5BPczxmRN48fTyrwcKSpyv2KNdK2 13, 33 -- W0XL8/SxOXqg8PgDFoVhxEBZ9TZsrn5b/aU/Izej2QeczhqsXeIJRl823yyBn06xojtw 13, 33 -- 5962nh/qN+cvNuAMS2kaJ4Qs4X5KkZsE5Yu40= 13, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 13, 33 -- d=gmail.com; s=gamma; 13, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 13, 33 -- :content-transfer-encoding; 13, 33 -- b=pC1N3kaDuT2LMo+hHbbl3vEr5LThYBX+02QLUXjDzqIsYkRQynBLiSPy1+kir+jJkP 13, 33 -- V67TxsL67s37+lThvRA/RomJoGvIf0gxK3Vo5Y8kv+vC6O7G9S1uf1mAo5m9ZdXcfdkq 13, 33 -- LQ7QpABMh94nA194wRQ1L6GVHI6Qw/kGBo/nc= 13, 33 -- MIME-Version: 1.0 13, 33 -- Received: by 10.42.171.134 with SMTP id j6mr152340icz.46.1294352624724; Thu, 13, 33 -- 06 Jan 2011 14:23:44 -0800 (PST) 13, 33 -- Received: by 10.42.218.130 with HTTP; Thu, 6 Jan 2011 14:23:44 -0800 (PST) 13, 33 -- Date: Thu, 6 Jan 2011 17:23:44 -0500 13, 33 -- Message-ID: {AANLkTinirXxELfYanarr2X9UMHEGvmJiJiUuhWOFbfBp-at-mail.gmail.com} 13, 33 -- Subject: Darkroom Equipment Free to Good Home 13, 33 -- From: Paula Sicurello {patpxs-at-gmail.com} 13, 33 -- To: Microscopy-at-microscopy.com 13, 33 -- Content-Type: text/plain; charset=ISO-8859-1 13, 33 -- Content-Transfer-Encoding: 8bit 13, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p06MNiSQ013108 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both r.sims-at-auckland.ac.nz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] JEOL 840 anode cap position
Message: Happy New Year
It's been a while since I pulled the anode cap for cleaning, and I can neither find the part in the manual where it says to use the upper or lower position for what accelerating voltage nor remember which height I used.
Can someone remind me which position should be used for 15KV operation?
cheers Ritchie
ps I can't seem to post in the normal way and our computer guy is on holiday. Something to do with hidden attachments, I think
For our 840A with tungsten emitter: use the upper position for 6kV accelerating voltage and *less*
use the lower position for over 6kV
Arcing occurs/can occur if the upper position is used with kV} 6kV.
Phil
} Email: r.sims-at-auckland.ac.nz } Name: Ritchie Sims } } Organization: The University of Auckland } } Title-Subject: [Filtered] JEOL 840 anode cap position } } Message: Happy New Year } } It's been a while since I pulled the anode cap for cleaning, and I } can neither find the part in the manual where it says to use the } upper or lower position for what accelerating voltage nor remember } which height I used. } } Can someone remind me which position should be used for 15KV operation? } } cheers } Ritchie } } ps I can't seem to post in the normal way and our computer guy is on } holiday. Something to do with hidden attachments, I think
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 26 -- From oshel1pe-at-cmich.edu Fri Jan 7 07:04:45 2011 7, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p07D4j6c001007 7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 07:04:45 -0600 7, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 26 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p07D4XtK019741; 7, 26 -- Fri, 7 Jan 2011 08:04:36 -0500 7, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 7, 26 -- Fri, 7 Jan 2011 08:02:29 -0500 7, 26 -- Mime-Version: 1.0 7, 26 -- Message-Id: {a06240800c94cbed85778-at-[141.209.160.249]} 7, 26 -- In-Reply-To: {201101070036.p070aigO004223-at-ns.microscopy.com} 7, 26 -- References: {201101070036.p070aigO004223-at-ns.microscopy.com} 7, 26 -- Date: Fri, 7 Jan 2011 08:02:26 -0500 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 26 -- Subject: Re: [Microscopy] viaWWW: JEOL 840 anode cap position 7, 26 -- Cc: r.sims-at-auckland.ac.nz 7, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 26 -- X-OriginalArrivalTime: 07 Jan 2011 13:02:29.0738 (UTC) FILETIME=[23E8BCA0:01CBAE6B] 7, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 26 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 7, 26 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 7, 26 -- X-CanItPRO-Stream: default 7, 26 -- X-Canit-Stats-ID: 02DQB4APT - be6aa4a98493 - 20110107 7, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I do not know the exact position of the anode but the "generic" conversion for a SEM is 1mm for every 2kV anode to cathode distance!
Hope this helps?
On another note I too have had then hidden attachment rejection on several occasion recently, could there be a problem?
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz] Sent: 07 January 2011 00:34 To: protrain-at-emcourses.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both r.sims-at-auckland.ac.nz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] JEOL 840 anode cap position
Message: Happy New Year
It's been a while since I pulled the anode cap for cleaning, and I can neither find the part in the manual where it says to use the upper or lower position for what accelerating voltage nor remember which height I used.
Can someone remind me which position should be used for 15KV operation?
cheers Ritchie
ps I can't seem to post in the normal way and our computer guy is on holiday. Something to do with hidden attachments, I think
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both r.sims-at-auckland.ac.nz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Out-of-Office auto replies
Message: Wow!
I got 21 out-of-office auto-replies to my JEOL 840 anode cap question, and no reply (yet) with information.
I guess I'll get another 21 auto-replies to this one, too.
May I quote the Listserver FAQs?
"2.) DO NOT set your Email Program to automatically reply to all messages, or to request a return receipt. If either of these are done, you run the risk of creating an Email loop within the system. This may result in a message bouncing through the system for several days, filling up everyone's mail box! "
I have found a brand new, still in the box with it's bubblewrap and packing peanuts, 11.5 inch inner diameter, 0.25 inch side wall Denton bell jar. I am giving it away, you pay the shipping.
Any takers?
Contact Andy Kloiber at andykloiber-at-yahoo.com
Have a greatr weekend!
Paula
Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#M251 Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
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I recently read this article about Ernst Ruska and thought of sharing it with the list, many of you may have already read this but it's truly a fascinating read.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
==============================Original Headers============================== 12, 35 -- From NEERAJG-at-clemson.edu Fri Jan 7 14:16:30 2011 12, 35 -- Received: from mailclient1.clemson.edu (mailclient1.clemson.edu [130.127.237.180]) 12, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p07KGUVo012098 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 14:16:30 -0600 12, 35 -- Received: from mailclient1.clemson.edu (localhost.clemson.edu [127.0.0.1]) 12, 35 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p07KGTlS026918 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 12, 35 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p07KGSJc026913 12, 35 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 12, 35 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Fri, 12, 35 -- 7 Jan 2011 15:16:28 -0500 12, 35 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 12, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 35 -- Date: Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Subject: Re: Ernst Ruska 12, 35 -- Thread-Topic: Re: Ernst Ruska 12, 35 -- Thread-Index: Acuup8SIOJpgV3B5RIecFUESkna64A== 12, 35 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F99385626E6-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 12, 35 -- Accept-Language: en-US 12, 35 -- Content-Language: en-US 12, 35 -- X-MS-Has-Attach: 12, 35 -- X-MS-TNEF-Correlator: 12, 35 -- acceptlanguage: en-US 12, 35 -- Content-Type: text/plain; charset="iso-8859-1" 12, 35 -- MIME-Version: 1.0 12, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 12, 35 -- definitions=2011-01-07_10:2011-01-07,2011-01-07,1970-01-01 signatures=0 12, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=2 12, 35 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 12, 35 -- engine=5.0.0-1012030000 definitions=main-1101070075 12, 35 -- Content-Transfer-Encoding: 8bit 12, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p07KGUVo012098 ==============================End of - Headers==============================
Two positions are available in electron microscopy at the Department of Materials, University of Oxford, UK:
Departmental Lecturer in Aberration Corrected Electron Microscopy Grade 8 / Salary Ł36,715 to Ł43,840 pa / Post Ref: DJ10/29
Senior Support Scientist in Electron Microscopy Grade 8 / Salary Ł36,715 to Ł43,840 pa / Post Ref: DJ10/30
See http://www.materials.ox.ac.uk/vacancies.html for further details.
Dr Peter Nellist {peter.nellist-at-materials.ox.ac.uk} University Lecturer in Materials Tutorial Fellow at Corpus Christi College Department of Materials University of Oxford Parks Road OXFORD OX1 3PH UK
Phone: +44 (0)1865-273656 Fax: +44 (0)1865-283329
Sent on behalf of Pete Nellist.
Mr. Ron Doole Department of Materials Senior Instrumentation Engineer. University of Oxford. Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
==============================Original Headers============================== 2, 28 -- From ron.doole-at-materials.ox.ac.uk Mon Jan 10 07:00:06 2011 2, 28 -- Received: from relay4.mail.ox.ac.uk (relay4.mail.ox.ac.uk [129.67.1.163]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0AD06Tm001381 2, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 10 Jan 2011 07:00:06 -0600 2, 28 -- Received: from smtp3.nexus.ox.ac.uk ([163.1.154.137] helo=exht03.ad.oak.ox.ac.uk) 2, 28 -- by relay4.mail.ox.ac.uk with esmtp (Exim 4.71) 2, 28 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} ) 2, 28 -- id 1PcHLy-0005Iq-Dy 2, 28 -- for Microscopy-at-Microscopy.Com; Mon, 10 Jan 2011 13:00:06 +0000 2, 28 -- Received: from EXMBX02.ad.oak.ox.ac.uk ([169.254.2.237]) by 2, 28 -- exht03.ad.oak.ox.ac.uk ([163.1.154.54]) with mapi; Mon, 10 Jan 2011 13:00:06 2, 28 -- +0000 2, 28 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk} 2, 28 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 2, 28 -- Date: Mon, 10 Jan 2011 13:00:05 +0000 2, 28 -- Subject: 2 TEM positions available at Oxford - UK 2, 28 -- Thread-Topic: 2 TEM positions available at Oxford - UK 2, 28 -- Thread-Index: AQHLsMZNsbXUBfxSZkGcITIyiqX9Xw== 2, 28 -- Message-ID: {FB563F1F5A8DDB49A34CB648BD23ACF932D1E38844-at-EXMBX02.ad.oak.ox.ac.uk} 2, 28 -- Accept-Language: en-US, en-GB 2, 28 -- Content-Language: en-GB 2, 28 -- X-MS-Has-Attach: 2, 28 -- X-MS-TNEF-Correlator: 2, 28 -- acceptlanguage: en-US, en-GB 2, 28 -- Content-Type: text/plain; charset="iso-8859-1" 2, 28 -- MIME-Version: 1.0 2, 28 -- Content-Transfer-Encoding: 8bit 2, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0AD06Tm001381 ==============================End of - Headers==============================
} Date: Mon, 10 Jan 2011 16:08:32 -0800 (PST) } From: Richard Kurtz {rkurtz-at-commack.k12.ny.us} } To: oshel1pe-at-cmich.edu } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Monday, January 10, } 2011 at 04:08:30 PM. } } realname - Richard Kurtz } Email - rkurtz-at-commack.k12.ny.us } ORGANIZATION - Commack High School } EDUCATION - 9-12th Grade High School } LOCATION - Commack, NY, USA } SUBJECT_OF_QUESTION - image of a insect foot pad } QUESTION - To Whom it may concern, } } I am a high school science teacher at Commack High School on Long Island, NY } I have a pair of students investigating the walking ability of the } Indian walking stick. The have the ability to walk on a host of } materials upside down and vertically. } } They and I wanted to find a place that would be able to look at } their footpads microscopically. } We are very interested in their surface, what structures are on } their feet. We want to know } were we may be able to find a place that would be able and willing } to help us with this. } } I have no idea how this could happen but just asking. } } Any advice or suggestions that you could provide would be great } } Thanks so much } -- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 1, 23 -- From oshel1pe-at-cmich.edu Tue Jan 11 10:56:39 2011 1, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0BGud3d028995 1, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 10:56:39 -0600 1, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 1, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0BGucD6001278 1, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 11:56:39 -0500 1, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 1, 23 -- Tue, 11 Jan 2011 11:56:38 -0500 1, 23 -- Mime-Version: 1.0 1, 23 -- Message-Id: {a06240804c9523bd6d418-at-[141.209.160.249]} 1, 23 -- Date: Tue, 11 Jan 2011 11:56:34 -0500 1, 23 -- To: Microscopy-at-microscopy.com 1, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 23 -- Subject: Fwd: Ask-A-Microscopist - SEM access needed, mid-Long Island NY 1, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 1, 23 -- X-OriginalArrivalTime: 11 Jan 2011 16:56:38.0766 (UTC) FILETIME=[836F48E0:01CBB1B0] 1, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 23 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 1, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 23 -- X-CanItPRO-Stream: default 1, 23 -- X-Canit-Stats-ID: 02DSgUCAP - d6b3e6a7655b - 20110111 1, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
This is something we could do as part of a Bugscope session, and I invite you to apply.
Thank you
Scott, with Bugscope
-- Microscopy Suite | Imaging Technology Group | Beckman Institute for Advanced Science and Technology | University of Illinois at Urbana-Champaign | 405 North Mathews Avenue, Urbana IL 61801 | 217 265 5071 | 217 244 6219 (fax) sjrobin-at-illinois.edu | http://itg.beckman.illinois.edu | http://bugscope.beckman.illinois.edu
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Tuesday, January 11, 2011 11:09 AM To: Robinson, Scott J.
} Date: Mon, 10 Jan 2011 16:08:32 -0800 (PST) } From: Richard Kurtz {rkurtz-at-commack.k12.ny.us} } To: oshel1pe-at-cmich.edu } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Monday, January 10, } 2011 at 04:08:30 PM. } } realname - Richard Kurtz } Email - } ORGANIZATION - Commack High School } EDUCATION - 9-12th Grade High School } LOCATION - Commack, NY, USA } SUBJECT_OF_QUESTION - image of a insect foot pad QUESTION - To Whom it } may concern, } } I am a high school science teacher at Commack High School on Long } Island, NY I have a pair of students investigating the walking ability } of the Indian walking stick. The have the ability to walk on a host of
} materials upside down and vertically. } } They and I wanted to find a place that would be able to look at their } footpads microscopically. } We are very interested in their surface, what structures are on their } feet. We want to know were we may be able to find a place that would } be able and willing to help us with this. } } I have no idea how this could happen but just asking. } } Any advice or suggestions that you could provide would be great } } Thanks so much } -- ************************************************************************ *************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ************************************************************************ ****************
==============================Original Headers============================== 1, 23 -- From oshel1pe-at-cmich.edu Tue Jan 11 10:56:39 2011 1, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0BGud3d028995 1, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 10:56:39 -0600 1, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 1, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0BGucD6001278 1, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 11:56:39 -0500 1, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 1, 23 -- Tue, 11 Jan 2011 11:56:38 -0500 1, 23 -- Mime-Version: 1.0 1, 23 -- Message-Id: {a06240804c9523bd6d418-at-[141.209.160.249]} 1, 23 -- Date: Tue, 11 Jan 2011 11:56:34 -0500 1, 23 -- To: Microscopy-at-microscopy.com 1, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 23 -- Subject: Fwd: Ask-A-Microscopist - SEM access needed, mid-Long Island NY 1, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 1, 23 -- X-OriginalArrivalTime: 11 Jan 2011 16:56:38.0766 (UTC) FILETIME=[836F48E0:01CBB1B0] 1, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 23 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 1, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 23 -- X-CanItPRO-Stream: default 1, 23 -- X-Canit-Stats-ID: 02DSgUCAP - d6b3e6a7655b - 20110111 1, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 23 -- From sjrobin-at-illinois.edu Tue Jan 11 11:15:35 2011 16, 23 -- Received: from BI-ADMEX.beckman.uiuc.edu (bi-admcls4.beckman.uiuc.edu [130.126.126.13]) 16, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0BHFZDD012798 16, 23 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 11:15:35 -0600 16, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 23 -- Content-class: urn:content-classes:message 16, 23 -- MIME-Version: 1.0 16, 23 -- Content-Type: text/plain; 16, 23 -- charset="us-ascii" 16, 23 -- Subject: RE: [Microscopy] Fwd: Ask-A-Microscopist - SEM access needed, mid-Long Island NY 16, 23 -- Date: Tue, 11 Jan 2011 11:13:16 -0600 16, 23 -- Message-ID: {87D63F6D93DB14418272BF5859FFF2C9139BA093-at-BI-ADMEX.beckman-admin.uiuc.edu} 16, 23 -- In-Reply-To: {201101111708.p0BH8ws9011457-at-ns.microscopy.com} 16, 23 -- X-MS-Has-Attach: 16, 23 -- X-MS-TNEF-Correlator: 16, 23 -- Thread-Topic: [Microscopy] Fwd: Ask-A-Microscopist - SEM access needed, mid-Long Island NY 16, 23 -- Thread-Index: AcuxskB6rr2OY6E+S/atT0dJ/Z4wLQAAEgVQ 16, 23 -- References: {201101111708.p0BH8ws9011457-at-ns.microscopy.com} 16, 23 -- From: "Robinson, Scott J." {sjrobin-at-illinois.edu} 16, 23 -- To: {oshel1pe-at-cmich.edu} , {microscopy-at-microscopy.com} 16, 23 -- Cc: {rkurtz-at-commack.k12.ny.us} , "bugscope" {bugscope-at-beckman.illinois.edu} 16, 23 -- Content-Transfer-Encoding: 8bit 16, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0BHFZDD012798 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lgiannuzzi-at-comcast.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] FIB/SEM Specialist: open position at Weatherford
Message: FIB-SEM Specialist Weatherford Laboratories Houston, TX Description: Weatherford Laboratories is seeking qualified candidates to fill the position of FIB-SEM Specialist at its Houston, Texas facility. The FIB-SEM Specialist is responsible for operating and maintaining a dual-column Focused Ion Beam (FIB) microscope / Scanning Electron Microscope (SEM) analytical instrument primarily to provide characterization of rock material from unconventional reservoirs. The FIB microscopy work includes in-situ high resolution imaging, micro-chemical analysis, and site-specific 3-D feature analysis, utilizing energy dispersive spectrometry (EDS) / secondary and backscatter electron characterization techniques. The FIB Microscopist will utilize their skills with dual-column FIB /electron microscopy to evaluate rock properties; perform data /image processing, including 3-D reconstruction and visualization of FIB imaging/analytical data; analyze and interpret comprehensive and multiple datasets; and communicate results to clients by oral and written reports. Qualifications: A bachelorís degree in a relevant scientific area is required, advanced degree preferred. Experience with operation of a dual-column FIB-SEM microscope is required. Image processing and geologic background a strong plus. Salary/Benefits: Commensurate with degree and experience. Company provides competitive benefit packages. Salary Range: Commensurate with degree and experience. Contact Information: Name: LouAnn Reid Company: Weatherford Laboratories Address: 5200 N. Sam Houston Pkwy W., Suite 500, Houston, TX 77086 Email: louann.reid-at-weatherfordlabs.com Website: www.weatherford.com to apply for position Open Search: 12/9/10 Close Search: Until filled
We are in the process of revising our recharge rates for our core microscopy facility. Although we do not solicit imaging projects from external customers, we do get some...usually from former students or companies associated with university faculty.
Our major instrumentation was purchased with the help of federal grants. The granting agencies do not have a problem with our using them for external projects provided that all internal clients funded by that agency, especially those whose projects were used for justification, have as much access as desired. However, they stipulate that any charges be equivalent to those of commercial providers.
Thus I need to talk to for-profit companies, who provide SEM and TEM, regarding current rates so that we can comply with this mandate. I would greatly appreciate your contacting me if you will be willing to help me in this task.
Thanks, Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 10, 28 -- From dsherman-at-purdue.edu Tue Jan 11 20:12:30 2011 10, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0C2CTKo025083 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 20:12:29 -0600 10, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 10, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0C2CTgD028619 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 21:12:29 -0500 10, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.164]) by WPPEXHUB01F.purdue.lcl 10, 28 -- ([::1]) with mapi; Tue, 11 Jan 2011 21:12:28 -0500 10, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 10, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 10, 28 -- Date: Tue, 11 Jan 2011 21:12:27 -0500 10, 28 -- Subject: Commercial microscopy rates 10, 28 -- Thread-Topic: Commercial microscopy rates 10, 28 -- Thread-Index: Acux/iiHU9Xf5zZHtEiQOde8ZXwsEA== 10, 28 -- Message-ID: {C952783B.AFE1%dsherman-at-purdue.edu} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- X-PMX-Version: 5.5.9.388399 10, 28 -- X-PerlMx-Virus-Scanned: Yes 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0C2CTKo025083 ==============================End of - Headers==============================
Hi all, We have someone who wants images of somatic cells (mainly leukocytes) in milk using light microscopy. I am looking for a staining method and would appreciate any hints to accomplish this. This is a bit out of my area with my being primarily an electron microscopist.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 6, 28 -- From dsherman-at-purdue.edu Tue Jan 11 20:15:54 2011 6, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0C2Fs9b032153 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 20:15:54 -0600 6, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 6, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0C2FsDk029105 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 21:15:54 -0500 6, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.164]) by WPPEXHUB02F.purdue.lcl 6, 28 -- ([::1]) with mapi; Tue, 11 Jan 2011 21:15:53 -0500 6, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 6, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 28 -- Date: Tue, 11 Jan 2011 21:15:51 -0500 6, 28 -- Subject: Staining somatic cells in milk 6, 28 -- Thread-Topic: Staining somatic cells in milk 6, 28 -- Thread-Index: Acux/qIfNhwwnZONaES65KSOZh3WKg== 6, 28 -- Message-ID: {C9527907.AFE6%dsherman-at-purdue.edu} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- X-PMX-Version: 5.5.9.388399 6, 28 -- X-PerlMx-Virus-Scanned: Yes 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0C2Fs9b032153 ==============================End of - Headers==============================
The main question is: can you isolate the cells, stain them and put them back in the milk?
If not, here are 2 possibilities:
The easiest one I can see is to incubate the sample with a DNA dye such as DAPI or Hoechst. These dyes are not expensive and are used by all microscopists doing fluorescence. Just mix the milk with a little bit of dye and observe...that's all! The only drawback is that you need a fluorescence microscope, but nowadays they are everywhere! I hope that the milk doesn't have an intrinsic autofluorescence in the UV range!
Alternatively tetrazolium salts can be metabolized by live cells into formazan, which are dyes. Some formazan dyes are soluble, others are not. Just choose an insoluble, like MTT. In this case you will only stain live cells, not the dead one. Tetrazolium salts are not expensive.
You won't be able to make the difference with germinal cells, though :-D I would avoid to use dyes which stain the membranes, since they may concentrate in the lipid droplets of the milk.
Regards, Stephane
----- Original Message ---- X-from: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu} To: nizets2-at-yahoo.com Sent: Wed, January 12, 2011 3:18:44 AM
Hi all, We have someone who wants images of somatic cells (mainly leukocytes) in milk using light microscopy. I am looking for a staining method and would appreciate any hints to accomplish this. This is a bit out of my area with my being primarily an electron microscopist.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 6, 28 -- From dsherman-at-purdue.edu Tue Jan 11 20:15:54 2011 6, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0C2Fs9b032153 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 20:15:54 -0600 6, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 6, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0C2FsDk029105 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 21:15:54 -0500 6, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.164]) by WPPEXHUB02F.purdue.lcl 6, 28 -- ([::1]) with mapi; Tue, 11 Jan 2011 21:15:53 -0500 6, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 6, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 28 -- Date: Tue, 11 Jan 2011 21:15:51 -0500 6, 28 -- Subject: Staining somatic cells in milk 6, 28 -- Thread-Topic: Staining somatic cells in milk 6, 28 -- Thread-Index: Acux/qIfNhwwnZONaES65KSOZh3WKg== 6, 28 -- Message-ID: {C9527907.AFE6%dsherman-at-purdue.edu} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- X-PMX-Version: 5.5.9.388399 6, 28 -- X-PerlMx-Virus-Scanned: Yes 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0C2Fs9b032153 ==============================End of - Headers==============================
==============================Original Headers============================== 25, 37 -- From nizets2-at-yahoo.com Wed Jan 12 05:50:11 2011 25, 37 -- Received: from nm26-vm0.bullet.mail.sp2.yahoo.com (nm26-vm0.bullet.mail.sp2.yahoo.com [98.139.91.230]) 25, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0CBoBCs008689 25, 37 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 05:50:11 -0600 25, 37 -- Received: from [98.139.91.66] by nm26.bullet.mail.sp2.yahoo.com with NNFMP; 12 Jan 2011 11:50:10 -0000 25, 37 -- Received: from [98.139.91.52] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 12 Jan 2011 11:50:10 -0000 25, 37 -- Received: from [127.0.0.1] by omp1052.mail.sp2.yahoo.com with NNFMP; 12 Jan 2011 11:50:10 -0000 25, 37 -- X-Yahoo-Newman-Property: ymail-3 25, 37 -- X-Yahoo-Newman-Id: 890075.12821.bm-at-omp1052.mail.sp2.yahoo.com 25, 37 -- Received: (qmail 70090 invoked by uid 60001); 12 Jan 2011 11:50:10 -0000 25, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1294833010; bh=dKrxBNFW4ytXLYCP+nF97/lTpfbjIbWO/CNk0eS6YLI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=q2x0SSV6hKeozJ/R2FDsG3Be7Bc1cL/sVY/Q2jF+WrWZyl1mT/+Es349sXBn5Ej5NBlXIoGkwQO9jy1f/8w2K9SEpLT2O8kiRweASJ9QpAWZyii7gF13zKLgsw7Vg9dHMMMLHTuY2YSvDVkLX/inAA8QzvQaT9WNEWFVScRBz0A= 25, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 25, 37 -- s=s1024; d=yahoo.com; 25, 37 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 25, 37 -- b=WTSHqBV9WqG6TdCWmU2Ukyvco5EtgGayejwD2oBbwtIAJwvw1Ur9SJO4JU1QMOVf7oTnvdANnGd9KopFMxzn6/0XLBmqU6ympJbPZrW8jc9A61gtLqro6+/0GY/gUEbfMchZ6lCQzUsdsoLtSnLfwK+/X6i/e51LwGTD3E6oOnA=; 25, 37 -- Message-ID: {218554.51777.qm-at-web110816.mail.gq1.yahoo.com} 25, 37 -- X-YMail-OSG: cMbsF_AVM1mSgmmzGAxvCz_JzlmJMeO2iKQLzx_I.kMfHoO 25, 37 -- MxtJ0BHEctYiA1dkyctHUsh.4mslgFpgRH5hDVrjWVC51aJidnclUQXItlgk 25, 37 -- mRWuBEErd5.7JQSZ8RjqYbiC5rFmEY7kXxJCEBQQ_LorYUopqnKzbfcLgjqv 25, 37 -- sTmqSi7Sveo6Rk7ixzVAGjjUz37IAjC3c6jihVk33zcUWc6VMd_pAi9hbQcW 25, 37 -- q3dkOAmBM9iC7VwgUud5W9S.Mwc_3P4vdz9R1dZeVOV4k8Q7SxAzZ01myUTG 25, 37 -- EMkG7XqdZgnQFvtXCZEyeYjD443V0tac8.8BF4qZpFFdoKvbHmqXQzfMr_7s 25, 37 -- UDR_jLSbGKPZ6N3HgtEoPauU1G2My2DfhxJEhye4VpNfY14m2CjXHlYkL2IV 25, 37 -- 6orYLno7cZQFrLWo- 25, 37 -- Received: from [80.122.101.100] by web110816.mail.gq1.yahoo.com via HTTP; Wed, 12 Jan 2011 03:50:09 PST 25, 37 -- X-Mailer: YahooMailRC/553 YahooMailWebService/0.8.107.285259 25, 37 -- References: {201101120218.p0C2Ii52009748-at-ns.microscopy.com} 25, 37 -- Date: Wed, 12 Jan 2011 03:50:09 -0800 (PST) 25, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 25, 37 -- Subject: Re: [Microscopy] Staining somatic cells in milk 25, 37 -- To: dsherman-at-purdue.edu 25, 37 -- Cc: microscopy-at-microscopy.com 25, 37 -- In-Reply-To: {201101120218.p0C2Ii52009748-at-ns.microscopy.com} 25, 37 -- MIME-Version: 1.0 25, 37 -- Content-Type: text/plain; charset=iso-8859-1 25, 37 -- Content-Transfer-Encoding: 8bit 25, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CBoBCs008689 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mseabul-at-aerotek.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Job Opening: Applications Scientist TEM/STEM of catalysts
Message: Hi! My name is Melanie and I work for Aerotek Scientific, a technical staffing firm in the Chicagoland area. I currently have an opening for a microscopy position in the area. Some details on this position are as follows:
-individual must be familiar with working with an STEM/TEM electron microscope, in support of the materials and catalyst development of the company -Individual will collaborate with and assist senior electron microscopists in the development of new capabilities for a new probe aberration corrected STEM/TEM microscope -Will be designing and conducting experiments and techniques using this microscope -operating the aberration corrected microscope for characterization of nanomaterials and catalysts -develope advanced applications for STEM, EELS, EDS tomography, and in-situ for beam sensitive nanomaterials
Best candidate will have Ph.D. in Materials Science, chemistry, physics or metallurgy with specialization in electron microscopy, experience with at least one of EDS, EELS, tomography or in-situ techniques and 0-5 years in a related field. Strong understanding of instrumentation and other characterization techniques such as X-ray diffraction are a plus.
Please let me know if you or anyone that you know may be interested and qualified for this position, I am actively seeking great candidates! Thank you!
Melanie Seabul Aerotek Scientific 1790 Nations Drive, Suite 116 Gurnee, IL 60031
Direct Line: 847-782-5447 E-mail: mseabul-at-aerotek.com
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Message: Hi! My name is Melanie and I work for Aerotek Scientific, a technical staffing firm in the Chicagoland area. I currently have a job opportunity for a Metallurgy/Corrosion scientist. Some details on this position are as follows:
-individual must have 10 years of on the job experience as a metallurgist and corrosion scientist -strong analytical chemistry skills -must have strong understanding of the degradation of metal at its surface through interaction with chemicals -working on project basis, researching and studying structures and chem. Properties of metals -experience working/running/analyzing the following tests: materials analysis, failure investigation, materials conservation, organic chemistry, analytical chemistry, petrographic evaluation -will be involved in running these tests: nondestructive evaluation, water penetration testing, strain and fracture monitoring, vibration monitoring, load testing, blast monitoring, frequency identification
The best candidate will have a masters degree, or higher, in a related scientific field with 10 years of experience. Please let me know if you or anyone that you know may be interested and qualified for this position as I am actively seeking great candidates! Thank you!
Melanie Seabul Aerotek Scientific 1790 Nations Drive, Suite 116 Gurnee, IL 60031
Direct Line: 847-782-5447 E-mail: mseabul-at-aerotek.com
As the article describes, the early version of the instrument could only reach modest resolutions and we have to remember that the thermal advancement Ultramicrotome by Porter and Bloom was reported in 1953. We know now what the combination of TEM and ultramicrotome did for biology and other fields, and gradually many crucial discoveries were made using the TEM which eventually could attain atomic resolutions. This is somewhat similar to discovery of LASER, many of the key people who worked on the LASER during its conception were awarded the Nobel prize later (Luckily the TEM didn't go through the lawsuits and patent battles that the LASER did). This may be true of our times too, we have yet to fully grasp the potential of recent advances in super-resolution optical imaging.
Best,
Neeraj.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
-----Original Message----- X-from: Carol Heckman [mailto:heckman-at-bgsu.edu] Sent: Sunday, January 09, 2011 12:00 PM To: Neeraj Gohad
Happy New Year Everyone!
I recently read this article about Ernst Ruska and thought of sharing it with the list, many of you may have already read this but it's truly a fascinating read.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
==============================Original Headers============================== 12, 35 -- From NEERAJG-at-clemson.edu Fri Jan 7 14:16:30 2011 12, 35 -- Received: from mailclient1.clemson.edu (mailclient1.clemson.edu [130.127.237.180]) 12, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p07KGUVo012098 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 14:16:30 -0600 12, 35 -- Received: from mailclient1.clemson.edu (localhost.clemson.edu [127.0.0.1]) 12, 35 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p07KGTlS026918 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 12, 35 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p07KGSJc026913 12, 35 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 12, 35 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Fri, 12, 35 -- 7 Jan 2011 15:16:28 -0500 12, 35 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 12, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 35 -- Date: Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Subject: Re: Ernst Ruska 12, 35 -- Thread-Topic: Re: Ernst Ruska 12, 35 -- Thread-Index: Acuup8SIOJpgV3B5RIecFUESkna64A== 12, 35 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F99385626E6-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 12, 35 -- Accept-Language: en-US 12, 35 -- Content-Language: en-US 12, 35 -- X-MS-Has-Attach: 12, 35 -- X-MS-TNEF-Correlator: 12, 35 -- acceptlanguage: en-US 12, 35 -- Content-Type: text/plain; charset="iso-8859-1" 12, 35 -- MIME-Version: 1.0 12, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 12, 35 -- definitions=2011-01-07_10:2011-01-07,2011-01-07,1970-01-01 signatures=0 12, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=2 12, 35 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 12, 35 -- engine=5.0.0-1012030000 definitions=main-1101070075 12, 35 -- Content-Transfer-Encoding: 8bit 12, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p07KGUVo012098 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 38 -- From NEERAJG-at-clemson.edu Wed Jan 12 13:01:16 2011 28, 38 -- Received: from mailclient2.clemson.edu (mailclient2.clemson.edu [130.127.237.181]) 28, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJ1F57006586 28, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 13:01:15 -0600 28, 38 -- Received: from mailclient2.clemson.edu (localhost.clemson.edu [127.0.0.1]) 28, 38 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0CJ1FoX021328 28, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:01:15 -0500 28, 38 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 28, 38 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0CJ1Ebd021322 28, 38 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 28, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:01:14 -0500 28, 38 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 28, 38 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Wed, 28, 38 -- 12 Jan 2011 14:01:14 -0500 28, 38 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 28, 38 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 28, 38 -- Date: Wed, 12 Jan 2011 14:01:12 -0500 28, 38 -- Subject: RE: [Microscopy] Re: Ernst Ruska 28, 38 -- Thread-Topic: [Microscopy] Re: Ernst Ruska 28, 38 -- Thread-Index: AcuuqRxxdQXGzIelSOiA0dPGxl0gdwBdYirHAJlPKZA= 28, 38 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938562BAC-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 28, 38 -- References: {201101072026.p07KQ4qq026821-at-ns.microscopy.com} 28, 38 -- {E76D862B01CC7845BF120D9591635FA0156D50-at-MAIL4.bgsu.edu} 28, 38 -- In-Reply-To: {E76D862B01CC7845BF120D9591635FA0156D50-at-MAIL4.bgsu.edu} 28, 38 -- Accept-Language: en-US 28, 38 -- Content-Language: en-US 28, 38 -- X-MS-Has-Attach: 28, 38 -- X-MS-TNEF-Correlator: 28, 38 -- acceptlanguage: en-US 28, 38 -- Content-Type: text/plain; charset="us-ascii" 28, 38 -- MIME-Version: 1.0 28, 38 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 28, 38 -- definitions=2011-01-12_05:2011-01-12,2011-01-12,1970-01-01 signatures=0 28, 38 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=8 28, 38 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 28, 38 -- engine=5.0.0-1012030000 definitions=main-1101120104 28, 38 -- Content-Transfer-Encoding: 8bit 28, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CJ1F57006586 ==============================End of - Headers==============================
Sorry, Neera - the Porter-Blum microtome was mechanical advance. Simple, reliable, unbreakable.
Caroline
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 3, 18 -- From schooley-at-mcn.org Wed Jan 12 13:22:17 2011 3, 18 -- Received: from smtp1.mcn.org (smtp1.mcn.org [216.150.240.85]) 3, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJMGbL022762 3, 18 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 13:22:17 -0600 3, 18 -- Received: from [66.81.41.72] 3, 18 -- by smtp1.mcn.org with esmtpa (Exim 4.63) 3, 18 -- (envelope-from {schooley-at-mcn.org} ) 3, 18 -- id 1Pd6Gq-0007FY-56; Wed, 12 Jan 2011 11:22:13 -0800 3, 18 -- Mime-Version: 1.0 3, 18 -- Message-Id: {a06200706c953adc1f12a-at-[66.81.41.72]} 3, 18 -- In-Reply-To: {201101121910.p0CJAjKW017068-at-ns.microscopy.com} 3, 18 -- References: {201101121910.p0CJAjKW017068-at-ns.microscopy.com} 3, 18 -- Date: Wed, 12 Jan 2011 11:21:19 -0800 3, 18 -- To: NEERAJG-at-clemson.edu 3, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 3, 18 -- Subject: Re: [Microscopy] Ernst Ruska 3, 18 -- Cc: {microscopy-at-microscopy.com} 3, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
To All Microscopists Researching High Resolution Biological Structure:
I call your attention to a new Special Issue of the Elsevier journal, MICRON, which has just been published (Volume 42, number 2: February, 2011). This originated from a realization that several different types of modern microscopes now can readily achieve atomic and sub-Ă…ngstrom levels of resolution with many inorganic specimens; since the resolution with biological specimens still only rarely obtains better than 3Ă… (0.3nm), this deficient resolution must not be caused by the instrument, but rather is due to inadequacies in specimen preparation and in structural preservation during data collection. These two important subjects are what the Special Issue deals with, in the context of a wide variety of biospecimens and use of several different kinds of microscopes (TEM, STEM, SEM, and AFM).
The 11 new review articles within this Special Issue concern the many practical problems for trying to produce better specimen preparation and preservation. Each is written by expert practicing microscopists. Many examples of research results and technical advances are illustrated by selected micrographs, diagrams, and charts, and are documented by extensive literature citations. Practical interest is expected from research students, technicians, and postdocs, as well as from experienced microscopists. We hope that these new reviews will be both informative and stimulating for all biological microscopists trying to obtain structural data with even higher resolution levels than are currently possible.
A complete table of contents follows this message. This Special Issue is included with all regular subscriptions to MICRON, and now is available to those at many institutions via the Science Direct website (http://www.sciencedirect.com).
Sincerely, and Happy New Year (2011),
BILL MASSOVER
William H. Massover Guest Editor, MICRON wmassover-at-anl.gov
Special Issue
MICRON Volume 42(2) February 2011: Contents
"Biological Specimen Preparation and Preservation for High Resolution Microscopies"
97 Introduction to Special Issue of Micron: “Biological specimen preparation and preservation for high resolution microscopies”
W.H. Massover
100 Obtaining high-resolution images of biological macromolecules by using a cryo-electron microscope with a liquid-helium cooled stage
K. Mitsuoka
107 Electron cryomicroscopy of membrane proteins: Specimen preparation for two-dimensional crystals and single particles
I. Schmidt-Krey and J.L. Rubinstein
117 Negative staining and cryo-negative staining of macromolecules and viruses for TEM
S. De Carlo and J. Robin Harris
132 Specimen preparation for electron diffraction of thin crystals
H. Wang and K.H. Downing
141 New and unconventional approaches for advancing resolution in biological transmission electron microscopy by improving macromolecular specimen preparation and preservation
W.H. Massover
152 Cryo-electron tomography on vitrified sections: A critical analysis of benefits and limitations for structural cell biology
C. Bouchet-Marquis and A. Hoenger
163 Preparation and high-resolution microscopy of gold cluster labeled nucleic acid conjugates and nanodevices
R.D. Powell and J.F. Hainfeld
175 Low voltage high-resolution SEM (LVHRSEM) for biological structural and molecular analysis
H. Schatten
186 Assessing the structure and function of single biomolecules with scanning transmission electron and atomic force microscopes
S.A. MĂĽller, D.J. MĂĽller and A. Engel
196 Preparation of DNA and nucleoprotein samples for AFM imaging
Y.L. Lyubchenko
==============================Original Headers============================== 41, 33 -- From wmassover-at-anl.gov Wed Jan 12 13:24:24 2011 41, 33 -- Received: from mailhost.anl.gov (mailhost.anl.gov [130.202.113.50]) 41, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJOO1n026491 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:24 -0600 41, 33 -- Received: from mailhost.anl.gov (mailhost.anl.gov [130.202.113.50]) 41, 33 -- by localhost.anl.gov (Postfix) with ESMTP id 483D854 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:24 -0600 (CST) 41, 33 -- Received: from zimbra.anl.gov (zimbra.anl.gov [130.202.101.12]) 41, 33 -- by mailhost.anl.gov (Postfix) with ESMTP id 2B5AD53 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:24 -0600 (CST) 41, 33 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 41, 33 -- by zimbra.anl.gov (Postfix) with ESMTP id 1887212437 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:24 -0600 (CST) 41, 33 -- X-Virus-Scanned: amavisd-new at zimbra.anl.gov 41, 33 -- Received: from zimbra.anl.gov ([127.0.0.1]) 41, 33 -- by localhost (zimbra.anl.gov [127.0.0.1]) (amavisd-new, port 10024) 41, 33 -- with ESMTP id vaJ92nLBKz-2 for {Microscopy-at-Microscopy.Com} ; 41, 33 -- Wed, 12 Jan 2011 13:24:24 -0600 (CST) 41, 33 -- Received: from zimbra.anl.gov (zimbra.anl.gov [130.202.101.12]) 41, 33 -- by zimbra.anl.gov (Postfix) with ESMTP id F330712436 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:23 -0600 (CST) 41, 33 -- Date: Wed, 12 Jan 2011 13:24:23 -0600 (CST) 41, 33 -- From: William Massover {wmassover-at-anl.gov} 41, 33 -- To: Microscopy-at-Microscopy.Com 41, 33 -- Message-ID: {2122403145.55417.1294860263970.JavaMail.root-at-zimbra.anl.gov} 41, 33 -- In-Reply-To: {1740785534.55378.1294859911603.JavaMail.root-at-zimbra.anl.gov} 41, 33 -- Subject: A New Resource for Trying to Improve Resolution with Biospecimens 41, 33 -- in TEM, STEM, SEM, and AFM 41, 33 -- MIME-Version: 1.0 41, 33 -- Content-Type: text/plain; charset=utf-8 41, 33 -- X-Originating-IP: [130.202.101.12] 41, 33 -- Content-Transfer-Encoding: 8bit 41, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CJOO1n026491 ==============================End of - Headers==============================
I did a few years looking at milk in-situ in mammary gland including lactating and involuting where we saw lots of somatic cells.
Hoechst or DAPI will work fine, there is not a significant problem with autofluorescence even in the UV range, and you can differentiate the types of cells a little by the staining pattern.
If you do not want to go the fluorescence root you could use plain old heamotoxylin and eosin. This has an added advantage, leucocytes are highly eosinophillic and are characterised very well with standard H&E. You will also get good ID on other cell types.
If you do not want spin down your cells you could try a standard blood smear prep on a coated slide and fix them on with formalin or paraformaldehyde. Alternatively you could add low melting point agar or agarose and set them as a gel. This will allow processing through paraffin if you need (although you lose your water and milk lipids of course.
Thanks
Ray
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, 13 January 2011 1:00 a.m. To: Ray Gilbert
Hi Debby!
The main question is: can you isolate the cells, stain them and put them back in the milk?
If not, here are 2 possibilities:
The easiest one I can see is to incubate the sample with a DNA dye such as DAPI or Hoechst. These dyes are not expensive and are used by all microscopists doing fluorescence. Just mix the milk with a little bit of dye and observe...that's all! The only drawback is that you need a fluorescence microscope, but nowadays they are everywhere! I hope that the milk doesn't have an intrinsic autofluorescence in the UV range!
Alternatively tetrazolium salts can be metabolized by live cells into formazan, which are dyes. Some formazan dyes are soluble, others are not. Just choose an insoluble, like MTT. In this case you will only stain live cells, not the dead one. Tetrazolium salts are not expensive.
You won't be able to make the difference with germinal cells, though :-D I would avoid to use dyes which stain the membranes, since they may concentrate in the lipid droplets of the milk.
Regards, Stephane
----- Original Message ---- X-from: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu} To: nizets2-at-yahoo.com Sent: Wed, January 12, 2011 3:18:44 AM
Hi all, We have someone who wants images of somatic cells (mainly leukocytes) in milk using light microscopy. I am looking for a staining method and would appreciate any hints to accomplish this. This is a bit out of my area with my being primarily an electron microscopist.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 6, 28 -- From dsherman-at-purdue.edu Tue Jan 11 20:15:54 2011 6, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0C2Fs9b032153 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 20:15:54 -0600 6, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 6, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0C2FsDk029105 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 21:15:54 -0500 6, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.164]) by WPPEXHUB02F.purdue.lcl 6, 28 -- ([::1]) with mapi; Tue, 11 Jan 2011 21:15:53 -0500 6, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 6, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 28 -- Date: Tue, 11 Jan 2011 21:15:51 -0500 6, 28 -- Subject: Staining somatic cells in milk 6, 28 -- Thread-Topic: Staining somatic cells in milk 6, 28 -- Thread-Index: Acux/qIfNhwwnZONaES65KSOZh3WKg== 6, 28 -- Message-ID: {C9527907.AFE6%dsherman-at-purdue.edu} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- X-PMX-Version: 5.5.9.388399 6, 28 -- X-PerlMx-Virus-Scanned: Yes 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0C2Fs9b032153 ==============================End of - Headers==============================
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My mistake, I meant mechanical, Porter-Blum MTI was indeed mechanical, but you get the point. What is the name of Ruska's book that you mentioned.
Best,
Neeraj.
-----Original Message----- X-from: schooley-at-mcn.org [mailto:schooley-at-mcn.org] Sent: Wednesday, January 12, 2011 2:27 PM To: Neeraj Gohad
Sorry, Neera - the Porter-Blum microtome was mechanical advance. Simple, reliable, unbreakable.
Caroline
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==============================Original Headers============================== 3, 18 -- From schooley-at-mcn.org Wed Jan 12 13:22:17 2011 3, 18 -- Received: from smtp1.mcn.org (smtp1.mcn.org [216.150.240.85]) 3, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJMGbL022762 3, 18 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 13:22:17 -0600 3, 18 -- Received: from [66.81.41.72] 3, 18 -- by smtp1.mcn.org with esmtpa (Exim 4.63) 3, 18 -- (envelope-from {schooley-at-mcn.org} ) 3, 18 -- id 1Pd6Gq-0007FY-56; Wed, 12 Jan 2011 11:22:13 -0800 3, 18 -- Mime-Version: 1.0 3, 18 -- Message-Id: {a06200706c953adc1f12a-at-[66.81.41.72]} 3, 18 -- In-Reply-To: {201101121910.p0CJAjKW017068-at-ns.microscopy.com} 3, 18 -- References: {201101121910.p0CJAjKW017068-at-ns.microscopy.com} 3, 18 -- Date: Wed, 12 Jan 2011 11:21:19 -0800 3, 18 -- To: NEERAJG-at-clemson.edu 3, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 3, 18 -- Subject: Re: [Microscopy] Ernst Ruska 3, 18 -- Cc: {microscopy-at-microscopy.com} 3, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 12, 37 -- From NEERAJG-at-clemson.edu Wed Jan 12 13:52:50 2011 12, 37 -- Received: from mailclient1.clemson.edu (mailclient1.clemson.edu [130.127.237.180]) 12, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJqoGn032605 12, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 13:52:50 -0600 12, 37 -- Received: from mailclient1.clemson.edu (localhost.clemson.edu [127.0.0.1]) 12, 37 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p0CJqo9Z016883 12, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:52:50 -0500 12, 37 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 12, 37 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p0CJqnwV016874 12, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 12, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:52:49 -0500 12, 37 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 12, 37 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Wed, 12, 37 -- 12 Jan 2011 14:52:49 -0500 12, 37 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 12, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 37 -- Date: Wed, 12 Jan 2011 14:52:47 -0500 12, 37 -- Subject: RE: [Microscopy] Re: Ernst Ruska 12, 37 -- Thread-Topic: [Microscopy] Re: Ernst Ruska 12, 37 -- Thread-Index: Acuyjrj9EOFUj3n9TsK7feja0s9AqQAA1l3w 12, 37 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938562C03-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 12, 37 -- References: {201101121927.p0CJR8Vl002739-at-ns.microscopy.com} 12, 37 -- In-Reply-To: {201101121927.p0CJR8Vl002739-at-ns.microscopy.com} 12, 37 -- Accept-Language: en-US 12, 37 -- Content-Language: en-US 12, 37 -- X-MS-Has-Attach: 12, 37 -- X-MS-TNEF-Correlator: 12, 37 -- acceptlanguage: en-US 12, 37 -- Content-Type: text/plain; charset="us-ascii" 12, 37 -- MIME-Version: 1.0 12, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 12, 37 -- definitions=2011-01-12_05:2011-01-12,2011-01-12,1970-01-01 signatures=0 12, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=8 12, 37 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 12, 37 -- engine=5.0.0-1012030000 definitions=main-1101120108 12, 37 -- Content-Transfer-Encoding: 8bit 12, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CJqoGn032605 ==============================End of - Headers==============================
The Electron Microscopy Core (EMC) at the University of Missouri Columbia, MO is proud to host an intensive short course:
Understanding and Optimizing FESEM and EDS Performance (Basic to Advanced)
Course Dates: Tuesday March 29 – Friday April 1, 2011 Cost: $1200 includes all course material (handouts and CD) and hands-on instrument instruction along with a reception and banquet Enrollment is limited to 12 participants!
Course Instructor: Steve Chapman - Protrain Steve Chapman formed Protrain in 1982 and is the senior consultant in electron microscopy. Steve first became involved with electron microscopes in 1964 at the University of Birmingham, England, then as an EM service engineer in the UK, and afterwards moved into sales and marketing where he worked with a number of electron microscope and accessory manufacturers in the demonstration and application fields. Running courses in many parts of the world, north and south of the equator, he has written six books including those on the operation and maintenance of scanning and transmission electron microscopes. His publications include papers on a range of electron microscopy subjects from instrument design through a wide range of applications and more recently on Quality in Electron Microscopy.
In a round-table illustrated discussion, you will be taken through the theory and practice of the operation of the scanning electron microscope, from basic through to advanced levels. In addition, the course will cover specimen preparation techniques and coating procedures. The EMC houses two field emission SEM’s, a Hitachi cold-field SEM (S-4700) and a FEI thermal FE SEM (Quanta 600 ESEM) with a Thermo System Six EDS. Practical periods will offer you the opportunity to evaluate instrument performance over a wide range of accelerating voltages and make appropriate modifications to the set-up in order to optimize the instrument’s performance. In this way, gun and specimen geometry may be better understood and you will be able to raise your skill levels to bring better performance from an instrument than the manufacturer claims. Other topics to be covered are low vacuum operation and imaging along with qualitative EDS analysis.
All students will operate the instruments themselves and develop their own talents. Under the eagle eye and guidance of Steve, participants should come away from this course with the understanding and abilities necessary to obtain the highest quality imaging and chemical analysis from their SEM/EDS systems.
Additional Support by: FEI, Hitachi High Technologies America, and Thermo Scientific.
For more Information or to register go to: http://www.emc.missouri.edu/ or contact me at rosslm-at-missouri.edu or at (573) 882-4777
Lou Ross -- Sr. Research Specialist University of Missouri Electron Microscopy Core Facility W136 Veterinary Medicine Building Columbia, MO 65211 573.882.4777, fax 573.884.2227 RossLM-at-missouri.edu http://www.emc.missouri.edu/
==============================Original Headers============================== 11, 31 -- From RossLM-at-missouri.edu Wed Jan 12 14:41:42 2011 11, 31 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CKfg6n019880 11, 31 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:41:42 -0600 11, 31 -- X-IronPort-Anti-Spam-Filtered: true 11, 31 -- X-IronPort-Anti-Spam-Result: AvsEAECgLU3RauUo/2dsb2JhbACkP3O2AIYYgw6CPgSEaIlO 11, 31 -- Received: from um-tsmtpout1.um.umsystem.edu ([209.106.229.40]) 11, 31 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 12 Jan 2011 14:41:38 -0600 11, 31 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 11, 31 -- Wed, 12 Jan 2011 14:41:38 -0600 11, 31 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 11, 31 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Wed, 12 Jan 2011 11, 31 -- 14:41:37 -0600 11, 31 -- From: "Ross Jr, Louis M." {RossLM-at-missouri.edu} 11, 31 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 31 -- Date: Wed, 12 Jan 2011 14:41:36 -0600 11, 31 -- Subject: 4-Day SEM Training Course - Univ of Missouri 3/29/11 11, 31 -- Thread-Topic: 4-Day SEM Training Course - Univ of Missouri 3/29/11 11, 31 -- Thread-Index: AcuymRrTj+6+mUpTJUalkakpihLZgw== 11, 31 -- Message-ID: {C9536E20.18C00%rosslm-at-missouri.edu} 11, 31 -- Accept-Language: en-US 11, 31 -- Content-Language: en-US 11, 31 -- X-MS-Has-Attach: 11, 31 -- X-MS-TNEF-Correlator: 11, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 11, 31 -- acceptlanguage: en-US 11, 31 -- Content-Type: text/plain; charset="Windows-1252" 11, 31 -- MIME-Version: 1.0 11, 31 -- X-OriginalArrivalTime: 12 Jan 2011 20:41:38.0272 (UTC) FILETIME=[1C2E2E00:01CBB299] 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CKfg6n019880 ==============================End of - Headers==============================
To follow up on what Caroline said, thermal advance was first brought out by LKB, and I believe was developed by Sjostrand.
Regards the Nobel Prize. Story I heard but could never get confirmed was that the failure to give Ruska the prize before the 1980's started as political, proceeded to oversight, and in the end became an embarrassment.
Ernst Ruska remained in Germany during WWII, and worked with the German war effort. Please remember that one of the major uses of the microscope in Germany and the US was in the development of the atomic bomb. Because of this, in the time leading up to, during and immediately after the war giving the prize to someone from Germany was not very acceptable. This was the story to me in 1969, when I first started doing EM. Ruska was, of course still alive, but as told, would not be a candidate for the prize.
Over the ensuing years Ruska sort of got forgotten. Then came the development of Scanning Tunneling EM. In 1986, when the Academy was considering the award of the Prize in Physics to Binnig and Rohrer they realized, much to many people's chagrin, that Ruska had never been recognized. Whatever his personal feelings may have been, his speech was gracious, and only said:
"Here, I only want to emphasize my impression that the scanning tunnel electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously been accepted much faster by scientific colleagues than electron microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech, December 10, 1986)
Sadly, the failure to act in a more timely fashion meant that others such as Hans Busch and Max Knoll (the only names that jump to mind just now) did not also receive the recognition they deserved.
Politics in the award of the Prize in all fields has been a long tradition. It still carries on. Witness the award of the Prize in Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to Henry Kissinger in 1973, without recognition of Nixon, who, for all the wrong things he did, directed the negotiations which lead to the end of the Viet Nam war and ultimately deserved to share in this recognition. And that is a heck of a statement for someone considered to be politically to the left of centre.
Paul Hazelton
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982
==============================Original Headers============================== 11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Jan 12 17:34:36 2011 11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CNYaRn008589 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 17:34:36 -0600 11, 20 -- Received: from [192.168.100.100] (wnpgmb014ww-ad06-64-92.dynamic.mts.net [207.161.64.92]) 11, 20 -- (user=hazeltn mech=LOGIN bits=0) 11, 20 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0CNYZt9015594; 11, 20 -- Wed, 12 Jan 2011 17:34:35 -0600 (CST) 11, 20 -- Message-ID: {4D2E3A92.9060205-at-umanitoba.ca} 11, 20 -- Date: Wed, 12 Jan 2011 17:34:42 -0600 11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 11, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 11, 20 -- MIME-Version: 1.0 11, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Ernst Ruska 11, 20 -- References: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 20 -- Content-Transfer-Encoding: 7bit 11, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
The title of the book is The early development of electron lenses and electron microscopy. The English translation was by Mulvey, and it was published in 1980.
My copy was borrowed 25 years ago, and I have not seen it since. It is long out of print.
paul hazelton
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982
==============================Original Headers============================== 4, 21 -- From paul_hazelton-at-umanitoba.ca Wed Jan 12 17:55:09 2011 4, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CNt95v024544 4, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 17:55:09 -0600 4, 21 -- Received: from [192.168.100.100] (wnpgmb014ww-ad06-64-92.dynamic.mts.net [207.161.64.92]) 4, 21 -- (user=hazeltn mech=LOGIN bits=0) 4, 21 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0CNt1f1024704; 4, 21 -- Wed, 12 Jan 2011 17:55:02 -0600 (CST) 4, 21 -- Message-ID: {4D2E3F5C.8060704-at-umanitoba.ca} 4, 21 -- Date: Wed, 12 Jan 2011 17:55:08 -0600 4, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 4, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 4, 21 -- MIME-Version: 1.0 4, 21 -- To: NEERAJG-at-clemson.edu 4, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 4, 21 -- Subject: Re: [Microscopy] Ernst Ruska 4, 21 -- References: {201101121953.p0CJrw53002740-at-ns.microscopy.com} 4, 21 -- In-Reply-To: {201101121953.p0CJrw53002740-at-ns.microscopy.com} 4, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 21 -- Content-Transfer-Encoding: 7bit 4, 21 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both brad-at-nanounity.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: brad-at-nanounity.com Name: Brad Rangell
Organization: NCSM
Title-Subject: [Filtered] NCSM kick-off meeting
Message: We are happy to announce the long anticipated reinstatement of the Northern California Chapter of the Society for Microscopy. Our first meeting will be an informal reception for you to get re-acquainted with your microscopy colleagues. During this get-together we want to begin to formalize membership and to obtain ideas for upcoming events for 2011. We anticipate the meeting to be a mix of networking and socializing, exchange of information and input from the membership on objectives and direction for this chapter. With this in mind here is the initial outline of the meeting. ...
Time: 6:00 to 8:00 PM
Location: International Building, SRI International, 333 Ravenswood Ave., Menlo Park, CA 94025; directions to follow.
Social hours: 6:00 to 7:00 PM hors de' oeuvres and beverages provided
Business items: 7:00 ń 8:00 Informal Discussion of the following topics:
ď Officer Elections ń we would like to ask members to provide suggestions of individuals to become elected officers. These can be submitted during the course of the evening. ď Discussion of By Laws for the society ď Discussion of future meetings and events in 2011 including subject matter and locations
Please send RSVP and questions to: Steve Samuelsson steven.samuelsson-at-sri.com or Brad Rangell brad-at-nanounity.com.
Please forward this notice to friends and colleagues who may have interest in joining NCSM and attending this kick-off meeting.
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Paul,
Thanks for sharing the story as well as the title of the book. The story is really interesting and sheds some light on why the prize was awarded so late. As we are all aware, any human endeavor is fraught with politics and I am sure the Nobel committees aren't immune to it. On the subject of Nobel piece prizes, a pretty major one they missed (according to the Nobel foundation itself) was Mahatma Gandhi (if any ones interested http://nobelprize.org/nobel_prizes/peace/articles/gandhi/ ).
Best Regards,
Neeraj.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: Wednesday, January 12, 2011 6:40 PM To: Neeraj Gohad
Neeraj et al
To follow up on what Caroline said, thermal advance was first brought out by LKB, and I believe was developed by Sjostrand.
Regards the Nobel Prize. Story I heard but could never get confirmed was that the failure to give Ruska the prize before the 1980's started as political, proceeded to oversight, and in the end became an embarrassment.
Ernst Ruska remained in Germany during WWII, and worked with the German war effort. Please remember that one of the major uses of the microscope in Germany and the US was in the development of the atomic bomb. Because of this, in the time leading up to, during and immediately after the war giving the prize to someone from Germany was not very acceptable. This was the story to me in 1969, when I first started doing EM. Ruska was, of course still alive, but as told, would not be a candidate for the prize.
Over the ensuing years Ruska sort of got forgotten. Then came the development of Scanning Tunneling EM. In 1986, when the Academy was considering the award of the Prize in Physics to Binnig and Rohrer they realized, much to many people's chagrin, that Ruska had never been recognized. Whatever his personal feelings may have been, his speech was gracious, and only said:
"Here, I only want to emphasize my impression that the scanning tunnel electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously been accepted much faster by scientific colleagues than electron microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech, December 10, 1986)
Sadly, the failure to act in a more timely fashion meant that others such as Hans Busch and Max Knoll (the only names that jump to mind just now) did not also receive the recognition they deserved.
Politics in the award of the Prize in all fields has been a long tradition. It still carries on. Witness the award of the Prize in Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to Henry Kissinger in 1973, without recognition of Nixon, who, for all the wrong things he did, directed the negotiations which lead to the end of the Viet Nam war and ultimately deserved to share in this recognition. And that is a heck of a statement for someone considered to be politically to the left of centre.
Paul Hazelton
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982
==============================Original Headers============================== 11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Jan 12 17:34:36 2011 11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CNYaRn008589 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 17:34:36 -0600 11, 20 -- Received: from [192.168.100.100] (wnpgmb014ww-ad06-64-92.dynamic.mts.net [207.161.64.92]) 11, 20 -- (user=hazeltn mech=LOGIN bits=0) 11, 20 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0CNYZt9015594; 11, 20 -- Wed, 12 Jan 2011 17:34:35 -0600 (CST) 11, 20 -- Message-ID: {4D2E3A92.9060205-at-umanitoba.ca} 11, 20 -- Date: Wed, 12 Jan 2011 17:34:42 -0600 11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 11, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 11, 20 -- MIME-Version: 1.0 11, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Ernst Ruska 11, 20 -- References: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 20 -- Content-Transfer-Encoding: 7bit 11, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
==============================Original Headers============================== 27, 37 -- From NEERAJG-at-clemson.edu Wed Jan 12 20:29:55 2011 27, 37 -- Received: from mailclient2.clemson.edu (mailclient2.clemson.edu [130.127.237.181]) 27, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0D2TtRS026507 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 20:29:55 -0600 27, 37 -- Received: from mailclient2.clemson.edu (localhost.clemson.edu [127.0.0.1]) 27, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0D2Ttmu009634 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 21:29:55 -0500 27, 37 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 27, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0D2Ts5P009631 27, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 21:29:54 -0500 27, 37 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 27, 37 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Wed, 27, 37 -- 12 Jan 2011 21:29:54 -0500 27, 37 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 27, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 27, 37 -- Date: Wed, 12 Jan 2011 21:29:52 -0500 27, 37 -- Subject: RE: [Microscopy] Re: Ernst Ruska 27, 37 -- Thread-Topic: [Microscopy] Re: Ernst Ruska 27, 37 -- Thread-Index: AcuysgUL2KFjAoUKSV6R1Ju/qF+IfAAFfX7Q 27, 37 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938562D32-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 27, 37 -- References: {201101122339.p0CNdoha016514-at-ns.microscopy.com} 27, 37 -- In-Reply-To: {201101122339.p0CNdoha016514-at-ns.microscopy.com} 27, 37 -- Accept-Language: en-US 27, 37 -- Content-Language: en-US 27, 37 -- X-MS-Has-Attach: 27, 37 -- X-MS-TNEF-Correlator: 27, 37 -- acceptlanguage: en-US 27, 37 -- Content-Type: text/plain; charset="us-ascii" 27, 37 -- MIME-Version: 1.0 27, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 27, 37 -- definitions=2011-01-13_01:2011-01-12,2011-01-13,1970-01-01 signatures=0 27, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=8 27, 37 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 27, 37 -- engine=5.0.0-1012030000 definitions=main-1101120181 27, 37 -- Content-Transfer-Encoding: 8bit 27, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0D2TtRS026507 ==============================End of - Headers==============================
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Paul,
Thanks for sharing the story as well as the title of the book. The story is really interesting and sheds some light on why the prize was awarded so late. As we are all aware, any human endeavor is fraught with politics and I am sure the Nobel committees aren't immune to it. On the subject of Nobel piece prizes, a pretty major one they missed (according to the Nobel foundation itself) was Mahatma Gandhi (if any ones interested http://nobelprize.org/nobel_prizes/peace/articles/gandhi/ ).
Best Regards,
Neeraj.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: Wednesday, January 12, 2011 6:40 PM To: Neeraj Gohad
Neeraj et al
To follow up on what Caroline said, thermal advance was first brought out by LKB, and I believe was developed by Sjostrand.
Regards the Nobel Prize. Story I heard but could never get confirmed was that the failure to give Ruska the prize before the 1980's started as political, proceeded to oversight, and in the end became an embarrassment.
Ernst Ruska remained in Germany during WWII, and worked with the German war effort. Please remember that one of the major uses of the microscope in Germany and the US was in the development of the atomic bomb. Because of this, in the time leading up to, during and immediately after the war giving the prize to someone from Germany was not very acceptable. This was the story to me in 1969, when I first started doing EM. Ruska was, of course still alive, but as told, would not be a candidate for the prize.
Over the ensuing years Ruska sort of got forgotten. Then came the development of Scanning Tunneling EM. In 1986, when the Academy was considering the award of the Prize in Physics to Binnig and Rohrer they realized, much to many people's chagrin, that Ruska had never been recognized. Whatever his personal feelings may have been, his speech was gracious, and only said:
"Here, I only want to emphasize my impression that the scanning tunnel electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously been accepted much faster by scientific colleagues than electron microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech, December 10, 1986)
Sadly, the failure to act in a more timely fashion meant that others such as Hans Busch and Max Knoll (the only names that jump to mind just now) did not also receive the recognition they deserved.
Politics in the award of the Prize in all fields has been a long tradition. It still carries on. Witness the award of the Prize in Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to Henry Kissinger in 1973, without recognition of Nixon, who, for all the wrong things he did, directed the negotiations which lead to the end of the Viet Nam war and ultimately deserved to share in this recognition. And that is a heck of a statement for someone considered to be politically to the left of centre.
Paul Hazelton
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982
==============================Original Headers============================== 11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Jan 12 17:34:36 2011 11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CNYaRn008589 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 17:34:36 -0600 11, 20 -- Received: from [192.168.100.100] (wnpgmb014ww-ad06-64-92.dynamic.mts.net [207.161.64.92]) 11, 20 -- (user=hazeltn mech=LOGIN bits=0) 11, 20 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0CNYZt9015594; 11, 20 -- Wed, 12 Jan 2011 17:34:35 -0600 (CST) 11, 20 -- Message-ID: {4D2E3A92.9060205-at-umanitoba.ca} 11, 20 -- Date: Wed, 12 Jan 2011 17:34:42 -0600 11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 11, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 11, 20 -- MIME-Version: 1.0 11, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Ernst Ruska 11, 20 -- References: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 20 -- Content-Transfer-Encoding: 7bit 11, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
==============================Original Headers============================== 27, 37 -- From NEERAJG-at-clemson.edu Wed Jan 12 20:29:55 2011 27, 37 -- Received: from mailclient2.clemson.edu (mailclient2.clemson.edu [130.127.237.181]) 27, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0D2TtRS026507 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 20:29:55 -0600 27, 37 -- Received: from mailclient2.clemson.edu (localhost.clemson.edu [127.0.0.1]) 27, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0D2Ttmu009634 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 21:29:55 -0500 27, 37 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 27, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0D2Ts5P009631 27, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 21:29:54 -0500 27, 37 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 27, 37 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Wed, 27, 37 -- 12 Jan 2011 21:29:54 -0500 27, 37 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 27, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 27, 37 -- Date: Wed, 12 Jan 2011 21:29:52 -0500 27, 37 -- Subject: RE: [Microscopy] Re: Ernst Ruska 27, 37 -- Thread-Topic: [Microscopy] Re: Ernst Ruska 27, 37 -- Thread-Index: AcuysgUL2KFjAoUKSV6R1Ju/qF+IfAAFfX7Q 27, 37 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938562D32-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 27, 37 -- References: {201101122339.p0CNdoha016514-at-ns.microscopy.com} 27, 37 -- In-Reply-To: {201101122339.p0CNdoha016514-at-ns.microscopy.com} 27, 37 -- Accept-Language: en-US 27, 37 -- Content-Language: en-US 27, 37 -- X-MS-Has-Attach: 27, 37 -- X-MS-TNEF-Correlator: 27, 37 -- acceptlanguage: en-US 27, 37 -- Content-Type: text/plain; charset="us-ascii" 27, 37 -- MIME-Version: 1.0 27, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 27, 37 -- definitions=2011-01-13_01:2011-01-12,2011-01-13,1970-01-01 signatures=0 27, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=8 27, 37 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 27, 37 -- engine=5.0.0-1012030000 definitions=main-1101120181 27, 37 -- Content-Transfer-Encoding: 8bit 27, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0D2TtRS026507 ==============================End of - Headers==============================
==============================Original Headers============================== 34, 28 -- From cross-at-tru.ca Wed Jan 12 22:20:08 2011 34, 28 -- Received: from mailgate.tru.ca (mailgate.tru.ca [192.146.156.111]) 34, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0D4K7r9011431 34, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 22:20:07 -0600 34, 28 -- Received: from mailgate.tru.ca (localhost.localdomain [127.0.0.1]) 34, 28 -- by mailgate (Postfix) with SMTP id 2E1E93246BF 34, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 20:20:05 -0800 (PST) 34, 28 -- Received: from Groupwise4.tru.ca (groupwise4.tru.ca [192.146.156.118]) 34, 28 -- by mailgate.tru.ca (Postfix) with ESMTP id 96EBB3246FE 34, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 20:20:04 -0800 (PST) 34, 28 -- Received: from TRUDOM4-MTA by Groupwise4.tru.ca 34, 28 -- with Novell_GroupWise; Wed, 12 Jan 2011 20:20:04 -0800 34, 28 -- Message-Id: {4D2E0CD9.BD8E.00AA.0-at-tru.ca} 34, 28 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 34, 28 -- Date: Wed, 12 Jan 2011 20:19:59 -0800 34, 28 -- From: "Cynthia Ross Friedman" {cross-at-tru.ca} 34, 28 -- To: {microscopy-at-microscopy.com} 34, 28 -- Subject: Re: [Microscopy] Ernst Ruska 34, 28 -- References: {201101130239.p0D2dCX7008495-at-ns.microscopy.com} 34, 28 -- In-Reply-To: {201101130239.p0D2dCX7008495-at-ns.microscopy.com} 34, 28 -- Mime-Version: 1.0 34, 28 -- Content-Type: text/plain; charset=US-ASCII 34, 28 -- Content-Disposition: inline 34, 28 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2011.1.13.40914 34, 28 -- X-PerlMx-Spam: Gauge=XII, Probability=12%, Report=' 34, 28 -- __CP_URI_IN_BODY! 0.5, TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, DATE_TZ_NA 0, FROM_NAME_PHRASE 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P1_5 0, __C230066_P5 0, __CD 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_BADTHINGS 0, __FRAUD_CONTACT_NUM 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0' 34, 28 -- Content-Transfer-Encoding: 8bit 34, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0D4K7r9011431 ==============================End of - Headers==============================
I've collected some light brown low density materials (of size about 1cm3) from cultural heritage object that mixed with clay. I've done FTIR and identified the material to be cellulose/wood. It was dispersed in water and then mount on microscopic glass slide. The particles are irregular in shape and measured 45-75 microns. I highly suspected they were termite frass. Is there any reference guide for identification of termite frass? They are coming from subtropical region in South East asia, that is Hong Kong. Thank you.
Regards, Wing Fai
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==============================Original Headers============================== 4, 25 -- From wflai-at-lcsd.gov.hk Thu Jan 13 03:15:39 2011 4, 25 -- Received: from sms01.lcsd.gov.hk (sms01.lcsd.gov.hk [203.31.32.70]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0D9FcuW001467 4, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 03:15:39 -0600 4, 25 -- Received: from sms01.lcsd.gov.hk (unknown [127.0.0.1]) 4, 25 -- by sms01.lcsd.gov.hk (Symantec Mail Security) with ESMTP id B352985801D 4, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:15:36 +0800 (HKT) 4, 25 -- X-AuditID: c0a80333-ad750bb000000a3a-19-4d2ec2b89451 4, 25 -- Received: from mail1.lcsd.gov.hk (unknown [10.47.1.65]) 4, 25 -- by sms01.lcsd.gov.hk (Symantec Mail Security) with ESMTP id 977607B8001 4, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:15:36 +0800 (HKT) 4, 25 -- To: Microscopy-at-microscopy.com 4, 25 -- MIME-Version: 1.0 4, 25 -- Subject: LM need help to identify termite frass 4, 25 -- X-KeepSent: DDDD9E4E:C0D7718D-48257817:00326434; 4, 25 -- type=4; name=$KeepSent 4, 25 -- X-Mailer: Lotus Notes Release 8.5.1 FP2 March 18, 2010 4, 25 -- Message-ID: {OFDDDD9E4E.C0D7718D-ON48257817.00326434-48257817.0032DDB1-at-lcsd.gov.hk} 4, 25 -- From: wflai-at-lcsd.gov.hk 4, 25 -- Date: Thu, 13 Jan 2011 17:15:31 +0800 4, 25 -- X-MIMETrack: Serialize by Router on notes_svr_2/SERVERS/LCSD/HKSARG(Release 8.5.1FP5|September 4, 25 -- 29, 2010) at 13.01.2011 05:15:36 PM, 4, 25 -- Serialize complete at 13.01.2011 05:15:36 PM 4, 25 -- Content-Type: text/plain; charset="US-ASCII" 4, 25 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
} Date: Thu, 13 Jan 2011 11:32:22 -0800 (PST) } From: Ronald Schwartz {ronmschwartz-at-yahoo.com} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Thursday, January 13, } 2011 at 11:32:20 AM. } } realname - Ronald Schwartz } Email - ronmschwartz-at-yahoo.com } ORGANIZATION - Providence, RI museum, US Fish&Wildlife Nature Center } EDUCATION - 6-8th Grade Middle School } LOCATION - Wakefield, RI } SUBJECT_OF_QUESTION - increasing microscope activities } QUESTION - I am a volunteer at the museum and the nature center. I } have been trying to initiate and expand microscope activities. I } volunteer my own equipment and my time. I would like to expand this } activity both where I volunteer and in the schools, but the } equipment is limited. Are there places that donate microscopes or } other equipment like cameras that link between the microscope and a } computer? The schools, esp. the middle schools, have little or no } equipment for either science class and/or extra-curricular activity. } When there are old scopes, teachers also don't seem to be trained in } using them.
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==============================Original Headers============================== 2, 23 -- From oshel1pe-at-cmich.edu Thu Jan 13 13:46:26 2011 2, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0DJkQkd002966 2, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 13:46:26 -0600 2, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 2, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0DJkJHK004452 2, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 14:46:26 -0500 2, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 2, 23 -- Thu, 13 Jan 2011 14:46:23 -0500 2, 23 -- Mime-Version: 1.0 2, 23 -- Message-Id: {a06240803c95506cb3a73-at-[141.209.160.249]} 2, 23 -- Date: Thu, 13 Jan 2011 14:46:18 -0500 2, 23 -- To: Microscopy-at-microscopy.com 2, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 23 -- Subject: Ask-A-Microscopist - donor 'scopes for schools? 2, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 2, 23 -- X-OriginalArrivalTime: 13 Jan 2011 19:46:23.0644 (UTC) FILETIME=[8EEBD5C0:01CBB35A] 2, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 23 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 2, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 23 -- X-CanItPRO-Stream: default 2, 23 -- X-Canit-Stats-ID: 02DT7Kpgw - 80b0de5056a9 - 20110113 2, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Anyone know of a source for these? I'm teaching an microscopy course and I'd like to show this classic demonstration. I got some cheap crystals from Carolina Biological but they aren't very transparent.
Gary Radice Department of Biology University of Richmond Richmond VA 23173 804-289-8107
==============================Original Headers============================== 4, 23 -- From gradice-at-richmond.edu Thu Jan 13 14:07:12 2011 4, 23 -- Received: from nylon.richmond.edu (nylon.richmond.edu [141.166.30.20]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0DK7BvU021921 4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 14:07:12 -0600 4, 23 -- Received: from polyester.richmond.edu (polyester.richmond.edu [141.166.24.28]) 4, 23 -- by nylon.richmond.edu (8.13.8/8.13.8) with ESMTP id p0DK7BSL010714 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 15:07:11 -0500 4, 23 -- Received: from ffa186132.richmond.edu (ffa186132.richmond.edu [141.166.186.132]) 4, 23 -- by polyester.richmond.edu (8.13.8/8.13.8) with ESMTP id p0DK79fl014015 4, 23 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 15:07:10 -0500 4, 23 -- Message-Id: {2F16F0CF-B866-446B-9628-314505BAC684-at-richmond.edu} 4, 23 -- From: gradice {gradice-at-richmond.edu} 4, 23 -- To: Microscopy-at-microscopy.com 4, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- Mime-Version: 1.0 (Apple Message framework v936) 4, 23 -- Subject: Source of calcite crystals for demonstrating birefringence? 4, 23 -- Date: Thu, 13 Jan 2011 15:07:09 -0500 4, 23 -- X-Mailer: Apple Mail (2.936) 4, 23 -- X-Spam-Detail: 0 4, 23 -- X-Scanned-By: MIMEDefang 2.67 on 141.166.24.28 ==============================End of - Headers==============================
A geology department will usually have transparent calcite on hand, but your institution does not appear to have that program. Transparent calcite is often referred to as "Iceland Spar." A quick search should turn up several potential suppliers. Wards, for example, has specimens in four different size/quality categories with prices that vary accordingly.
Regards,
- Steve Kuehn
gradice-at-richmond.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Anyone know of a source for these? I'm teaching an microscopy course } and I'd like to show this classic demonstration. I got some cheap } crystals from Carolina Biological but they aren't very transparent. } } } Gary Radice } Department of Biology } University of Richmond } Richmond VA 23173 } 804-289-8107
--
---------------- Dr. Stephen C. Kuehn Research Scientist and Lecturer Manager, Electron Microprobe Facility Division of Natural Sciences Science, room 106
Concord University, Campus Box F20 1000 Vermillion St PO Box 1000 Athens, WV 24712-1000
Bart Cannon {cannonmp-at-comcast.net} just asked me to pass along that he has available plenty of inexpensive, colorless "iceland spar" suitable for demonstration purposes.
Regards,
- Steve
} } Gary, } } A geology department will usually have transparent calcite on hand, but } your institution does not appear to have that program. Transparent } calcite is often referred to as "Iceland Spar." A quick search should } turn up several potential suppliers. Wards, for example, has specimens } in four different size/quality categories with prices that vary } accordingly. } } Regards, } } - Steve Kuehn
--
---------------- Dr. Stephen C. Kuehn Research Scientist and Lecturer Manager, Electron Microprobe Facility Division of Natural Sciences Science, room 106
Concord University, Campus Box F20 1000 Vermillion St PO Box 1000 Athens, WV 24712-1000
So I did a little digging on who reported thermal advance in ultramicrotomes first (not which commercial instruments came first) the original paper by Keith Porter and J. Blum published in 1953, A study in Microtomy for electron Microscopy, The Anatomical Record Volume 117, Issue 4, pages 685-709, December 1953, in it Porter and Blum describe two new mechanisms, one is a thermal expansion and the other is mechanical advancement (I have a PDF of this paper if anyone is interested). Here is an excerpt from the Summary of this paper
'Two new microtomes capable of cutting serial sections as thin as 25-50 mu are described. All moving parts in these instruments are supported in unlubricated pivots and the specimen is taken past the cutting edge only on the cutting stroke. These two features more than any others seem responsible for the successful performance of these microtomes. In one instrument, the prototype, the block is advanced to the knife by thermal expansion. In the other, the advancement is controlled mechanically.'
Interestingly enough, Fritiof Sjostrand's paper, A new microtome for ultrathin sectioning for high resolution microscopy, 1953 Experientia 9:114-121 (note the paper also published in 1953) says 'The microtome reported on in this paper represents a further development of the microtome designed by Porter' (http://www.springerlink.com/content/y3267k5456j11117/).
Also found an interesting book, Picture Control: The Electron Microscope and the Transformation of Biology in America by Nicolas Rasmussen, has some interesting history on this matter (see preview, http://books.google.com/books?id=rwC5QiqLS44C&pg=PA127&lpg=PA127&dq=Sjostrand+a+new+ultramicrotome&source=bl&ots=HCTXlObRJ-&sig=G46YfqxY8lrFtBptZDgmxIDbxuA&hl=en&ei=cHAvTfLRBsKSgQfMo-xa&sa=X&oi=book_result&ct=result&resnum=3&ved=0CCwQ6AEwAg#v=onepage&q&f=false
Pretty Interesting!
Best,
Neeraj.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
X-from: Paul Hazelton [mailto:hazeltn-at-cc.umanitoba.ca] Sent: Wednesday, January 12, 2011 4:09 PM To: Neeraj Gohad
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Ron -
Bravo for your efforts! Are you aware that 1) MSA has regional societies, & 2) the New England Society has kits for middle school microscopy that they circulate to people like you? Their program chair is Mary McCann {mccanns-at-tiac.net} , (617)484-7865. If you have difficulty reaching her, let me know.
I hope that you'll visit the Project MICRO web page (URL below). There's a lot of advice about suitable equipment. Donated scopes can be more of a liability than an asset; they are too complex for middle school teachers & they usually need parts & service.
We're here to help; please ask questions. I'm a retiree, which means that I have the time to respond to your Email!
Caroline -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America 45301 Caspar Point Road, Box 117 Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 6, 18 -- From schooley-at-mcn.org Thu Jan 13 17:49:32 2011 6, 18 -- Received: from smtp2.mcn.org (smtp2.mcn.org [216.150.240.86]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0DNnW28025218 6, 18 -- for {microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:49:32 -0600 6, 18 -- Received: from [66.81.50.60] 6, 18 -- by smtp2.mcn.org with esmtpa (Exim 4.63) 6, 18 -- (envelope-from {schooley-at-mcn.org} ) 6, 18 -- id 1PdWuz-0001Du-4D; Thu, 13 Jan 2011 15:49:26 -0800 6, 18 -- Mime-Version: 1.0 6, 18 -- Message-Id: {a06200702c9553af52ab6-at-[66.81.43.189]} 6, 18 -- In-Reply-To: {201101131956.p0DJuH7j015484-at-ns.microscopy.com} 6, 18 -- References: {201101131956.p0DJuH7j015484-at-ns.microscopy.com} 6, 18 -- Date: Thu, 13 Jan 2011 15:48:58 -0800 6, 18 -- To: oshel1pe-at-cmich.edu 6, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 6, 18 -- Subject: Re: [Microscopy] Ask-A-Microscopist - donor 'scopes for schools? 6, 18 -- Cc: microscopy-at-microscopy.com 6, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
We're in the process of writing a proposal for a new SEM, and will likely include a cathodoluminescence detector. As I don't know a whole lot about these, I'd like some input on performance, brands, models, prices, company service (especially service) and the like. Use will be for materials and especially mineralogy research and teaching in our microscopy program. So the unit needs to be suitable for research, but robust and user-friendly enough for teaching.
This is not for a NSF or such grant, so cost is also important (low cost, whatever that is for a good cathodoluminescence detector). But I don't have a good handle on prices yet - other than that there are some hideously expensive units that we can't consider. Vendor replies welcome.
BUT! **Off-line** please, not to the list.
Although if someone wants to start a discuss on the technique and uses of cathodoluminescence, I'd be interested in reading it ...
Thanks.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 23 -- From oshel1pe-at-cmich.edu Fri Jan 14 07:58:33 2011 7, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0EDwXFd013018 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 07:58:33 -0600 7, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0EDwJFD009286 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 08:58:33 -0500 7, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 7, 23 -- Fri, 14 Jan 2011 08:57:31 -0500 7, 23 -- Mime-Version: 1.0 7, 23 -- Message-Id: {a06240804c95604cdfa23-at-[141.209.160.249]} 7, 23 -- Date: Fri, 14 Jan 2011 08:57:28 -0500 7, 23 -- To: Microscopy-at-microscopy.com 7, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 23 -- Subject: cathodoluminescence detectors 7, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 23 -- X-OriginalArrivalTime: 14 Jan 2011 13:57:32.0005 (UTC) FILETIME=[FD1AF150:01CBB3F2] 7, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 23 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,RDNS_NONE,Bayes(0.0001:-0.5) 7, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 7, 23 -- X-CanItPRO-Stream: default 7, 23 -- X-Canit-Stats-ID: 02DTpWxSy - c2f15745d688 - 20110114 7, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
First! Please reply directly to me, **not** the list.
We are hoping to get a new silicon drift detector (SDD) EDS system for our SEM. Either as a retro-fit or for a new SEM. I've been looking at the models available, but I'd like to get some user input - reliability, user-friendliness, company service, is the software open-source or proprietary, and how much does the company charge for software upgrades? EDS software integration with SEM software/hardware, energy resolution, light element abilities - all the usual things, but as actual user experiences.
Is the system tied to a particular computer hardware design? (Our current system uses an old proprietary circuit board that uses an obsolete slot/bus, so it can't be upgraded or moved to a new computer, the whole thing has to be replaced.)
This will be for both research - materials, mineralogy, biology, and whatever else comes up - and teaching.
Vendor replies welcome. To me, please, not the list.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 23 -- From oshel1pe-at-cmich.edu Fri Jan 14 08:08:02 2011 7, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0EE82HY025267 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 08:08:02 -0600 7, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0EE7eXR010183 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 09:08:02 -0500 7, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 7, 23 -- Fri, 14 Jan 2011 09:06:44 -0500 7, 23 -- Mime-Version: 1.0 7, 23 -- Message-Id: {a06240803c95604c5f849-at-[141.209.160.249]} 7, 23 -- Date: Fri, 14 Jan 2011 09:06:41 -0500 7, 23 -- To: Microscopy-at-microscopy.com 7, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 23 -- Subject: SDD detectors 7, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 23 -- X-OriginalArrivalTime: 14 Jan 2011 14:06:44.0801 (UTC) FILETIME=[4698EB10:01CBB3F4] 7, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 23 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,RDNS_NONE,Bayes(0.0001:-0.5) 7, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 7, 23 -- X-CanItPRO-Stream: default 7, 23 -- X-Canit-Stats-ID: 02DTq82WV - d70aee24b0f7 - 20110114 7, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
} Date: Fri, 14 Jan 2011 07:20:51 -0800 (PST) } From: Norah Silva {norah-at-norahsilva.com} } Subject: Ask-A-Microscopist - Photomicroscopy training in Florida? } } Below is the result of your form, submitted on Friday, January 14, } 2011 at 07:20:43 AM. } } realname - Norah Silva } Email - norah-at-norahsilva.com } EDUCATION - Undergraduate College } LOCATION - Boca Raton, FL 33432 } SUBJECT_OF_QUESTION - Capturing images } QUESTION - Hello, } I am a photographer and am going back to school for my BS and am } very interested in the field of microscopy as well as recomendations } of where to learn more about capture systems. Are there devices } compatible with Hasselblad or Canon digital capture systems? } What is the typical line of study to get in to this field? } } Thank you in advance for your advice, } Norah Silva } -- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 1, 23 -- From oshel1pe-at-cmich.edu Fri Jan 14 12:01:41 2011 1, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0EI1fG4005497 1, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 12:01:41 -0600 1, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 1, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0EI1cwl001804 1, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 13:01:41 -0500 1, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 1, 23 -- Fri, 14 Jan 2011 13:00:55 -0500 1, 23 -- Mime-Version: 1.0 1, 23 -- Message-Id: {a06240818c9563f9bc26e-at-[141.209.160.249]} 1, 23 -- Date: Fri, 14 Jan 2011 13:00:51 -0500 1, 23 -- To: Microscopy-at-microscopy.com 1, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 23 -- Subject: Fwd: Ask-A-Microscopist - Photomicroscopy training in Florida? 1, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 1, 23 -- X-OriginalArrivalTime: 14 Jan 2011 18:00:55.0574 (UTC) FILETIME=[FD82BB60:01CBB414] 1, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 23 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 1, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 23 -- X-CanItPRO-Stream: default 1, 23 -- X-Canit-Stats-ID: 02DTu1FGE - d8187a12bc04 - 20110114 1, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
A post-doctoral position is available in the LeBeau group at North Carolina State University. The successful candidate will be responsible for the development of scanning transmission electron microscopy techniques in conjunction with studying interfaces, nanoparticles/nanocomposites, and device structures. The candidate will focus on quantitative electron microscopy, determination of heterogeneous interface structures, and exploring chemistry with spectroscopic techniques. Applicants should have demonstrated expertise in problem solving in materials science using a wide range of transmission electron microscopy techniques.
NCSU will soon acquire an aberration corrected microscope for ultra-high resolution STEM imaging and chemical analysis. Other equipment includes a JEOL 2010 STEM/TEM, several conventional TEMs, and a FIB. The position is available starting Jan 1, 2011 and requires a Ph. D. in materials science or a related field. Experience with aberration corrected STEM is desired. Duration is between 1-3 years and salary is commensurate with qualifications.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vish.bhakthavatsalam-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Microscopist with a phD in chemistry
Message: A minimum Ph.D. in Physical Sciences, Analytical Chemistry, Material Science or related discipline ď Experience A minimum of 2-3 years of Hands ńon instrument experience in SEM-EDS, EBSD, TEM are required in the industry. Experience with materials used in the mining/metal industry is a bonus; A background in mineralogy and hands on experience in characterization techniques thereof (SEM-EDS, EBSD, TEM, AFM, sputter coating, sample preparation using ultra microtome) is required. Experience in surface science processes and techniques (e.g. AFM, STM) will be considered especially advantageous.
ď Mandatory skills Good communication and presentation skills must be evident; peer review publications in international journals and presentations at significant analytical conferences is a plus. Erase this text and type your question here
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Email: erwrigh-at-emory.edu Name: Elizabeth Wright
Organization: Emory University
Title-Subject: [Filtered] Postdoctoral Position Available
Message: A postdoctoral fellow position is available immediately with the Spearman and Wright laboratories at Emory University to study HIV assembly and trafficking using fluorescence microscopy and cryo-EM methods.
Motivated candidates with strong interests in HIV biology and cryo-electron microscopy of complex molecular assemblies are encouraged to apply. Research experience in the following area(s) is desired: 1) retrovirology, 2) cryo-EM, and/or 3) fluorescence microscopy.
Our laboratories are within the Division of Pediatric Infectious Diseases and are fully equipped for all aspects of molecular virology, molecular biology and protein biochemistry. The Spearman lab has a Deltavision deconvolution microscopy station and a spinning disk confocal system both equipped for live cell imaging, FRAP, photoactivation, and now for cryo-imaging. The Wright lab has complete access to a JEOL 2200FS 200 kV FEG TEM with an in-column energy filter, Zernike phase plates, and high-resolution 4kx4k CCD camera; a JEOL 1400 120 kV TEM with 2kx2k CCD camera; and an FEI Vitrobot and a manual grid plunge freezer. Emory is a vibrant, multidisciplinary campus with strong ties to Georgia Tech and other universities within Georgia. Access to facilities in NMR, Mass Spectrometry, and X-ray crystallography are available.
Applicants should include a cover letter describing research experience and interests, curriculum vitae, and names and contact information for three references.
Dr. Elizabeth R. Wright Emory University Department of Pediatrics 2015 Uppergate Drive, NE Room: 548 Atlanta, GA 30322 erwrigh-at-emory.edu Website: http://electronmicroscopy.emory.edu
Applications will be reviewed as they are received and until the position is filled.
We have an old CI-46 water jacketed CO2 incubator that was donated years ago. Someone wants to brush the dust off and try using this because they want to avoid contaminating our main incubators with something they want to grow.
Our problem though is that we no longer have contact with the group that donated the incubator I think, and they didn't include a manual. Does anyone have a manual laying around or basic instructions for use? None of the ports on the backside are labeled anymore, and I don't know if American Scientific Products got absorbed by another company I could possibly call or did they just dissolve or do some other action causing it to be a problem tracking them down. I'm a youngling.
On the backside I just see three ports, one is the power, one is something electrical, and the other looks like a water siphon port. I'm assuming the nut on the front when you open the door might be the fill for the water jacket. We don't see where the CO2 is attached anywhere and their is a rather larger hole through the back of the chamber and we don't see anything that should fill that hole given to us along with the incubator. I guess they were afraid the cells might be scared of the dark?
Help in either getting us an old manual or contact for who might still be servicing these machines would be helpful. Not quite microscopy but assuming a few people are still around biology labs or maybe a colleague they know. Thanks and enjoy the weekend.
Off topic for this, thanks for those of you on the Ernst Ruska thread. Some interesting informative posts about history are always a good read.
-Jason Saredy University of North Florida
==============================Original Headers============================== 7, 27 -- From sarj0007-at-unf.edu Sat Jan 15 13:02:12 2011 7, 27 -- Received: from relay4.unf.edu (relay4.unf.edu [139.62.192.127]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0FJ2Cm7006325 7, 27 -- for {microscopy-at-microscopy.com} ; Sat, 15 Jan 2011 13:02:12 -0600 7, 27 -- X-IronPort-Anti-Spam-Filtered: true 7, 27 -- X-IronPort-Anti-Spam-Result: AvsEAH59MU2LPsCK/2dsb2JhbACkaXO1aIZqhVAEhHA 7, 27 -- Received: from callisto1.unfcsd.unf.edu ([139.62.192.138]) 7, 27 -- by relay1.unf.edu with ESMTP; 15 Jan 2011 14:02:11 -0500 7, 27 -- Received: from jupiter.unfcsd.unf.edu ([169.254.1.119]) by 7, 27 -- callisto1.unfcsd.unf.edu ([139.62.192.138]) with mapi; Sat, 15 Jan 2011 7, 27 -- 14:02:11 -0500 7, 27 -- From: "Saredy, Jason" {sarj0007-at-unf.edu} 7, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 27 -- Date: Sat, 15 Jan 2011 14:02:10 -0500 7, 27 -- Subject: ci-46 incubator 7, 27 -- Thread-Topic: ci-46 incubator 7, 27 -- Thread-Index: AQHLtOa22xn9Zcp58UKhBJKltFcEiw== 7, 27 -- Message-ID: {68A0416A3B94A542B9164AE7A32DE1DE2F30B71F22-at-JUPITER.unfcsd.unf.edu} 7, 27 -- Accept-Language: en-US 7, 27 -- Content-Language: en-US 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- acceptlanguage: en-US 7, 27 -- Content-Type: text/plain; charset="iso-8859-1" 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0FJ2Cm7006325 ==============================End of - Headers==============================
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Email: Michael.Cammer-at-med.nyu.edu Name: Michael Cammer
Message: We have the Nikon TIRF system and have three laser lines going into the TIRF arm via a single fiber. When we project through the 100X objective through the sample onto the wall we see that the lines go through the sample at different angles. (You can see a picture of the projection at approx 45 degrees at http://www.flickr.com/photos/mcammer/5359189090/ .) It is also noticeable in the TIRF images that the field depth is different for each wavelength. Is this unavoidable due to the different wavelengths or is it possible to align the optics better so these spots would be more coincident?
That's a beautiful photograph of the unavoidable relationship between the angle of incidence and the wavelength of light used to illuminate the sample. It stems from the fact that the relative index of refraction of the glass-water interface is slightly different for for each wavelength. To ensure that the penetration depth is nearly equal for all laser lines, you would have to offset each laser beam to a different specific radial position on the back focal plane of the objective - and these relative spacings would change as well if you wanted a different penetration depth. You can work out what these spacings need to be by examining the basic equation for the TIRF penetration depth (you have to assume you know the wavelength dependent index of refraction of the sample though which ranges from 1.33 to 1.38) and you'll find that they are on the order of a few tens of micrometers depending on the specific penetration depth you desire (see http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfintro.html). There's a nice little ImageJ plugin that can be used:
http://rsbweb.nih.gov/ij/plugins/tirf/index.html
John Oreopoulos Research Assistant Spectral Applied Research 9078 Leslie Street, Unit 11 Richmond Hill, Ontario Canada
On 2011-01-16, at 10:37 AM, Michael.Cammer-at-med.nyu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both Michael.Cammer-at-med.nyu.edu as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: Michael.Cammer-at-med.nyu.edu } Name: Michael Cammer } } Organization: Skirball Langone NYU Med Center } } Title-Subject: [Filtered] TIRF laser alignment question } } Message: We have the Nikon TIRF system and have three laser lines } going into the TIRF arm via a single fiber. When we project through } the 100X objective through the sample onto the wall we see that the } lines go through the sample at different angles. (You can see a } picture of the projection at approx 45 degrees at } http://www.flickr.com/photos/mcammer/5359189090/ .) It is also } noticeable in the TIRF images that the field depth is different for } each wavelength. Is this unavoidable due to the different } wavelengths or is it possible to align the optics better so these } spots would be more coincident? } } Thank you. } } Sincerely, } } Michael Cammer } } Login Host: 96.246.240.120 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 22 -- From zaluzec-at-microscopy.com Sun Jan 16 09:30:29 2011 } 9, 22 -- Received: from znl.com ([206.69.208.20]) } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0GFUTMI000864 } 9, 22 -- for {microscopy-at-microscopy.com} ; Sun, 16 Jan 2011 09:30:29 -0600 } 9, 22 -- Received: from localhost (localhost [127.0.0.1]) } 9, 22 -- by znl.com (Postfix) with ESMTP id 9DE9B313DDB } 9, 22 -- for {microscopy-at-microscopy.com} ; Sun, 16 Jan 2011 09:30:29 -0600 (CST) } 9, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 9, 22 -- Received: from znl.com ([127.0.0.1]) } 9, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 9, 22 -- with ESMTP id UiKkrOX5x+kx for {microscopy-at-microscopy.com} ; } 9, 22 -- Sun, 16 Jan 2011 09:30:29 -0600 (CST) } 9, 22 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 22 -- by znl.com (Postfix) with ESMTPA id CF970313DCB } 9, 22 -- for {microscopy-at-microscopy.com} ; Sun, 16 Jan 2011 09:30:28 -0600 (CST) } 9, 22 -- Mime-Version: 1.0 } 9, 22 -- Message-Id: {p06240801c958bf8365ef-at-[206.69.208.22]} } 9, 22 -- Date: Sun, 16 Jan 2011 09:30:26 -0600 } 9, 22 -- To: microscopy-at-microscopy.com } 9, 22 -- From: Michael.Cammer-at-med.nyu.edu (by way of MicroscopyListserver) } 9, 22 -- Subject: viaWWW: TIRF laser alignment question } 9, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 28 -- From john.oreopoulos-at-utoronto.ca Sun Jan 16 10:30:17 2011 9, 28 -- Received: from bureau61.ns.utoronto.ca (bureau61.ns.utoronto.ca [128.100.132.151]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0GGUHLW018522 9, 28 -- for {Microscopy-at-microscopy.com} ; Sun, 16 Jan 2011 10:30:17 -0600 9, 28 -- Received: from [192.168.2.13] (bas3-toronto06-2925097395.dsl.bell.ca [174.89.113.179] (may be forged)) 9, 28 -- (authenticated bits=0) 9, 28 -- by bureau61.ns.utoronto.ca (8.13.8/8.13.8) with ESMTP id p0GGUDK7017003 9, 28 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 9, 28 -- Sun, 16 Jan 2011 11:30:15 -0500 9, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/simple; d=utoronto.ca; s=beta; 9, 28 -- t=1295195416; bh=LxPipE27XvQW9QQHRyw/C8uclP9Psf8CCabI44i6C7c=; 9, 28 -- h=Content-Type:Mime-Version:Subject:From:In-Reply-To:Date: 9, 28 -- Content-Transfer-Encoding:Message-Id:References:To; 9, 28 -- b=BIQ1TDiX05nv3YUpUiAQxh6KNAaUASTi5En1S0UvG31FM1WdIMcdQGbmepza/cppb 9, 28 -- AfuYv8I/F5YXbZ3ec5JwrDrbTZQphrWjpC64BhIAL3AQbG4XsdKDRcUYmXd3woLwpc 9, 28 -- 5OG5OeED1Nx9W84vIwEYWEZNYRS5aL9Bp5MXm+EA= 9, 28 -- Content-Type: text/plain; charset=us-ascii 9, 28 -- Mime-Version: 1.0 (Apple Message framework v1082) 9, 28 -- Subject: Re: [Microscopy] viaWWW: TIRF laser alignment question 9, 28 -- From: John Oreopoulos {john.oreopoulos-at-utoronto.ca} 9, 28 -- In-Reply-To: {201101161537.p0GFbWHh008327-at-ns.microscopy.com} 9, 28 -- Date: Sun, 16 Jan 2011 11:30:13 -0500 9, 28 -- Message-Id: {BA43080F-F9E9-4657-BBD0-84AF19314BF3-at-utoronto.ca} 9, 28 -- References: {201101161537.p0GFbWHh008327-at-ns.microscopy.com} 9, 28 -- To: Michael.Cammer-at-med.nyu.edu, Microscopy-at-microscopy.com 9, 28 -- X-Mailer: Apple Mail (2.1082) 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0GGUHLW018522 ==============================End of - Headers==============================
Thanks for the reply. Reading it and the referenced websites jogged my memory.
A few years ago we were having problems with the first commercial Olympus TIRF system because we could not get consistent evanescent waves with the one angle adjustment with the laser lines we had from 405 to 568 nm that were delivered via a single fiber (it was worse when we later added a 633 or 638 nm laser). I suggested we pump each laser in through a separate path that could be angled independently. We didn't build it, but I think Olympus now sells a TIRF system that does this.
Another issue is that when I first heard about TIRF maybe 15 years ago, it was introduced as a ring illumination at the outer edge of the back aperture, not as a single point or crescent at the periphery on only one side. A ring, or at least a series of points around the periphery, seems like a better way to provide a uniform field due to aberrations from coherent light in the imperfect optics. Any thought on this?
Sincerely,
Michael
_________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
________________________________________ X-from: John Oreopoulos [john.oreopoulos-at-utoronto.ca] Sent: Sunday, January 16, 2011 11:30 AM To: Cammer, Michael; Microscopy-at-microscopy.com
Dear Listers,
I am posting this on the request of Bill Caufman from Intersil Company in Palm Bay, Florida. Bill needs help with reviving an EDX system with "Voyager" electronics made by Noran and needs technical assistance.
If someone can provide technical help or even on-site assistance for this kind of EDX system in Palm Bay Florida - please respond directly to Bill at
BCAUFFMA-at-intersil.com
Thank you very much beforehand, -- Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com
==============================Original Headers============================== 5, 32 -- From vray-at-partbeamsystech.com Mon Jan 17 21:19:19 2011 5, 32 -- Received: from nm16-vm0.bullet.mail.ac4.yahoo.com (nm16-vm0.bullet.mail.ac4.yahoo.com [98.139.52.238]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0I3JIfD015751 5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 17 Jan 2011 21:19:18 -0600 5, 32 -- Received: from [98.139.52.193] by nm16.bullet.mail.ac4.yahoo.com with NNFMP; 18 Jan 2011 03:19:18 -0000 5, 32 -- Received: from [98.139.52.142] by tm6.bullet.mail.ac4.yahoo.com with NNFMP; 18 Jan 2011 03:19:18 -0000 5, 32 -- Received: from [127.0.0.1] by omp1025.mail.ac4.yahoo.com with NNFMP; 18 Jan 2011 03:19:18 -0000 5, 32 -- X-Yahoo-Newman-Id: 235624.18271.bm-at-omp1025.mail.ac4.yahoo.com 5, 32 -- Received: (qmail 36405 invoked from network); 18 Jan 2011 03:19:17 -0000 5, 32 -- Received: from [172.18.10.172] (vray-at-211.72.229.241 with plain) 5, 32 -- by smtp112.biz.mail.mud.yahoo.com with SMTP; 17 Jan 2011 19:19:17 -0800 PST 5, 32 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 32 -- X-YMail-OSG: s0fHlvoVM1kvkA6GtZygbox2jxTjpzTZ8sm26XeoHvo.N20 5, 32 -- f.o8hb2_9BtxQ7DZAlmwTO1pi3lghMbrDwtRIfxRPNt3wsakSFTjReZWEXjX 5, 32 -- 09OgtrP6XtrsrFvekc5fTVsRsZEjZk5h4q2.N8ML0.ZjT2V7.bPrgEqlKrbt 5, 32 -- XLUYWd_J42r__31VEfYPZggeZwCxyj4bcABcKFDBgLf8laDY.8fuiAwMk1fs 5, 32 -- oAt_oq.DiafbxbG2mf8E5DeBdXSTCvUoD0s5vFEgbnG3J5KgkL72nhksQMO_ 5, 32 -- rsKWr1t01B41gMd3WEfOPMHoHRgPeu4mpTaklH0pe_bZ79Y84jMAG6gdCo8R 5, 32 -- XnJRKfaw6pS61eM.sM1ANFuZrIPkY_Bk3sAL5HK14Rpjn 5, 32 -- X-Yahoo-Newman-Property: ymail-3 5, 32 -- Message-ID: {4D3506B4.4060802-at-partbeamsystech.com} 5, 32 -- Date: Mon, 17 Jan 2011 22:19:16 -0500 5, 32 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 5, 32 -- Reply-To: vray-at-partbeamsystech.com 5, 32 -- Organization: PBS&T 5, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Lightning/1.0b2 Thunderbird/3.1.7 5, 32 -- MIME-Version: 1.0 5, 32 -- To: microscopy-at-microscopy.com 5, 32 -- CC: "Cauffman, Bill \(bcauffma\)" {BCAUFFMA-at-intersil.com} 5, 32 -- Subject: Help with Noran/Voyager EDX in Florida 5, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On Sun, 16 Jan 2011 19:19:44 +0100, {Michael.Cammer-at-med.nyu.edu} wrote:
} Another issue is that when I first heard about TIRF maybe 15 years ago, } it was introduced as a ring illumination at the outer edge of the back } aperture, not as a single point or crescent at the periphery on only one } side. A ring, or at least a series of points around the periphery, } seems like a better way to provide a uniform field due to aberrations } from coherent light in the imperfect optics. Any thought on this?
Yes, you are right. See e.g. this paper about this very topic: Fiolka, R., Belyaev, Y., Ewers, H., & Stemmer, A. (2008). Even illumination in total internal reflection fluorescence microscopy using laser light. Microscopy research and technique, 71(1), 45-50. doi: 10.1002/jemt.20527.
Cheers Janne
==============================Original Headers============================== 7, 19 -- From Janne.Hyoetylae-at-stud.unibas.ch Tue Jan 18 07:38:23 2011 7, 19 -- Received: from smtp2.unibas.ch (smtp2.unibas.ch [131.152.227.82]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0IDcMsH020835 7, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Jan 2011 07:38:23 -0600 7, 19 -- X-IronPort-AV: E=Sophos;i="4.60,339,1291590000"; 7, 19 -- d="scan'208";a="117112964" 7, 19 -- Received: from bioz3-mihp55.mih.unibas.ch (HELO moria) ([131.152.19.124]) 7, 19 -- by smtp2priv.unibas.ch with ESMTP; 18 Jan 2011 14:38:13 +0100 7, 19 -- Content-Type: text/plain; charset=utf-8; format=flowed; delsp=yes 7, 19 -- To: Michael.Cammer-at-med.nyu.edu, Microscopy-at-microscopy.com 7, 19 -- Subject: Re: TIRF laser alignment question 7, 19 -- References: {201101161819.p0GIJilA016381-at-ns.microscopy.com} 7, 19 -- Date: Tue, 18 Jan 2011 14:38:12 +0100 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- From: =?utf-8?Q?Janne_Hy=C3=B6tyl=C3=A4?= {Janne.Hyoetylae-at-stud.unibas.ch} 7, 19 -- Message-ID: {op.vpiatyrsxxlwia-at-moria} 7, 19 -- In-Reply-To: {201101161819.p0GIJilA016381-at-ns.microscopy.com} 7, 19 -- User-Agent: Opera Mail/11.00 (Linux) ==============================End of - Headers==============================
The Deben stage controls on the SEM are getting a little stiff, and the maintenance manual recommends Santovac 5 for lubrication. Too bad, I don't have any around, and I don't relish the thought of forking over several hundred dollars for an entire bottle just to use a miniscule bit. Has anybody out there recently serviced a DP and have an "empty" Santovac container that I could squeeze a few drops out of? I'll spring for shipping, plus offer sacrifices to the gods of EM on your behalf for good vibes and round electrons.
Thanks in advance,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
If you don't get any from others in the Great White North, let me know, I can send you some. If we can get it past NTSA and Customs and ...
Phil Hm. "GWN" is somewhat more than the land of the Maple Leaf right now.
} Greetings listers, } } The Deben stage controls on the SEM are getting a little stiff, and the } maintenance manual recommends Santovac 5 for lubrication. Too bad, I } don't have any around, and I don't relish the thought of forking over } several hundred dollars for an entire bottle just to use a miniscule } bit. Has anybody out there recently serviced a DP and have an "empty" } Santovac container that I could squeeze a few drops out of? I'll spring } for shipping, plus offer sacrifices to the gods of EM on your behalf for } good vibes and round electrons. } } Thanks in advance, } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB E4L 1G7 } CANADA } } phone: 506-364-2519 } fax: 506-364-2505 } email: jehrman-at-mta.ca } www: http://www.mta.ca/dmf } } Svent-Gyorgyi's Axiom: } Discovery consists of seeing what everybody } has seen and thinking what nobody thought.
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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In 1963, ten years after the papers were publlished, I joined the Lab of Dan Moore, at Rockefeller. That lab, which had been Porter's until he moved to Harvard, was located in the basement of Smith Hall, and actually extended under 68th street. At any rate, I learned microtomy on one of the mechanical advance microtomes that were built in the machine shop --I still have it--, and there was also a thermal advance version. The thermal advancement was provided by an incandescent light bulb suspended over the cutting arm. You advanced the block by flashing the light. As you might expect, the area around the microtome was forbidden to others while it was in use. Any air currents would disturb the whole process.
Joel
On Thu, Jan 13, 2011 at 5:26 PM, {NEERAJG-at-clemson.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } So I did a little digging on who reported thermal advance in ultramicrotomes first (not which commercial instruments came first) the original paper by Keith Porter and J. Blum published in 1953, A study in Microtomy for electron Microscopy, The Anatomical Record Volume 117, Issue 4, pages 685-709, December 1953, in it Porter and Blum describe two new mechanisms, one is a thermal expansion and the other is mechanical advancement (I have a PDF of this paper if anyone is interested). Here is an excerpt from the Summary of this paper } } 'Two new microtomes capable of cutting serial sections as thin as 25-50 mu are described. All moving parts in these instruments are supported in unlubricated pivots and the specimen is taken past the cutting edge only on the cutting stroke. These two features more than any others seem responsible for the successful performance of these microtomes. In one instrument, the prototype, the block is advanced to the knife by thermal expansion. In the other, the advancement is controlled mechanically.' } } Interestingly enough, Fritiof Sjostrand's paper, A new microtome for ultrathin sectioning for high resolution microscopy, 1953 Experientia 9:114-121 (note the paper also published in 1953) says 'The microtome reported on in this paper represents a further development of the microtome designed by Porter' (http://www.springerlink.com/content/y3267k5456j11117/). } } Also found an interesting book, Picture Control: The Electron Microscope and the Transformation of Biology in America by Nicolas Rasmussen, has some interesting history on this matter (see preview, http://books.google.com/books?id=rwC5QiqLS44C&pg=PA127&lpg=PA127&dq=Sjostrand+a+new+ultramicrotome&source=bl&ots=HCTXlObRJ-&sig=G46YfqxY8lrFtBptZDgmxIDbxuA&hl=en&ei=cHAvTfLRBsKSgQfMo-xa&sa=X&oi=book_result&ct=result&resnum=3&ved=0CCwQ6AEwAg#v=onepage&q&f=false } } } Pretty Interesting! } } Best, } } Neeraj. } } } Neeraj V. Gohad, Ph.D. } Postdoctoral Fellow } Okeanos Research Group } Department of Biological Sciences } 132 Long Hall } Clemson University } Clemson,SC-29634 } Phone: 864-656-3597 } Fax: 864-656-0435 } } Website: http://www.clemson.edu/okeanos } } } } } } } } } } } } X-from: Paul Hazelton [mailto:hazeltn-at-cc.umanitoba.ca] } Sent: Wednesday, January 12, 2011 4:09 PM } To: Neeraj Gohad } Subject: Re: [Microscopy] Ernst Ruska } } Neeraj et al } } To follow up on what Caroline said, thermal advance was first brought out by LKB, and I believe was developed by Sjostrand. } } Regards the Nobel Prize. Story I heard but could never get confirmed was that the failure to give Ruska the prize before the 1980's started as political, proceeded to oversight, and in the end became an embarrassment. } } Ernst Ruska remained in Germany during WWII, and worked with the German war effort. Please remember that one of the major uses of the microscope in Germany and the US was in the development of the atomic bomb. Because of this, in the time leading up to, during and immediately after the war giving the prize to someone from Germany was not very acceptable. This was the story to me in 1969, when I first started doing EM. Ruska was, of course still alive, but as told, would not be a candidate for the prize. } } Over the ensuing years Ruska sort of got forgotten. Then came the development of Scanning Tunneling EM. In 1986, when the Academy was considering the award of the Prize in Physics to Binnig and Rohrer they realized, much to many people's chagrin, that Ruska had never been recognized. Whatever his personal feelings may have been, his speech was gracious, and only said: } } "Here, I only want to emphasize my impression that the scanning tunnel electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously been accepted much faster by scientific colleagues than electron microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech, December 10, 1986) } } Sadly, the failure to act in a more timely fashion meant that others such as Hans Busch and Max Knoll (the only names that jump to mind just now) did not also receive the recognition they deserved. } } Politics in the award of the Prize in all fields has been a long tradition. It still carries on. Witness the award of the Prize in Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to Henry Kissinger in 1973, without recognition of Nixon, who, for all the wrong things he did, directed the negotiations which lead to the end of the Viet Nam war and ultimately deserved to share in this recognition. And that is a heck of a statement for someone considered to be politically to the left of centre. } } Paul Hazelton } } -- } } Paul R. Hazelton, PhD } Gastroenteric Diseases Study Group } University of Manitoba } Department of Medical Microbiology } 511 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 3J7 } e-mail: paul_hazelton-at-umanitoba.ca } Phone: 204-789-3313 (w) } 204-489-6924 (h) } Cell: 204-781-6982 } Fax: 204-789-3926 } } } ==============================Original Headers============================== } 35, 38 -- From NEERAJG-at-clemson.edu Thu Jan 13 16:18:28 2011 } 35, 38 -- Received: from mailclient3.clemson.edu (mailclient3.clemson.edu [130.127.237.182]) } 35, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0DMIShU007551 } 35, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 16:18:28 -0600 } 35, 38 -- Received: from mailclient3.clemson.edu (localhost.clemson.edu [127.0.0.1]) } 35, 38 -- by mailclient3.clemson.edu (8.13.8/8.13.8) with ESMTP id p0DMIR13007594 } 35, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:18:27 -0500 } 35, 38 -- Received: from frontex-2.CAMPUS.CU.CLEMSON.EDU (frontex-2.clemson.edu [130.127.235.60]) } 35, 38 -- by mailclient3.clemson.edu (8.13.8/8.13.8) with ESMTP id p0DMIQut007585 } 35, 38 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) } 35, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:18:26 -0500 } 35, 38 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by } 35, 38 -- frontex-2.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb3c::827f:eb3c]) with mapi; Thu, } 35, 38 -- 13 Jan 2011 17:18:26 -0500 } 35, 38 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} } 35, 38 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 35, 38 -- Date: Thu, 13 Jan 2011 17:18:26 -0500 } 35, 38 -- Subject: RE: [Microscopy] Ernst Ruska } 35, 38 -- Thread-Topic: [Microscopy] Ernst Ruska } 35, 38 -- Thread-Index: AcuynOdoI32UWIhFRl6VmRIQcrDJMAAzMIHw } 35, 38 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938625FE3-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} } 35, 38 -- References: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} } 35, 38 -- {4D2E1851.4040202-at-cc.umanitoba.ca} } 35, 38 -- In-Reply-To: {4D2E1851.4040202-at-cc.umanitoba.ca} } 35, 38 -- Accept-Language: en-US } 35, 38 -- Content-Language: en-US } 35, 38 -- X-MS-Has-Attach: } 35, 38 -- X-MS-TNEF-Correlator: } 35, 38 -- acceptlanguage: en-US } 35, 38 -- Content-Type: text/plain; charset="iso-8859-1" } 35, 38 -- MIME-Version: 1.0 } 35, 38 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 } 35, 38 -- definitions=2011-01-13_09:2011-01-13,2011-01-13,1970-01-01 signatures=0 } 35, 38 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=2 } 35, 38 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx } 35, 38 -- engine=5.0.0-1012030000 definitions=main-1101130148 } 35, 38 -- Content-Transfer-Encoding: 8bit } 35, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0DMIShU007551 } ==============================End of - Headers============================== }
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 7, 35 -- From joelsheffield-at-gmail.com Tue Jan 18 16:20:08 2011 7, 35 -- Received: from mail-wy0-f169.google.com (mail-wy0-f169.google.com [74.125.82.169]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0IMK7qv031430 7, 35 -- for {microscopy-at-microscopy.com} ; Tue, 18 Jan 2011 16:20:08 -0600 7, 35 -- Received: by wyj26 with SMTP id 26so162455wyj.0 7, 35 -- for {microscopy-at-microscopy.com} ; Tue, 18 Jan 2011 14:20:07 -0800 (PST) 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=domainkey-signature:mime-version:in-reply-to:references:date 7, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 7, 35 -- bh=4Ux6VAoDeCHPyVoM9Y72oMaxs+yPFsDcnJZCxedVFx8=; 7, 35 -- b=q0Djo0tfKkXTsB1HyRqTSAkxttUaLtYeElyAP1334TWduBfvweNRRnAoEXAc1bWwPQ 7, 35 -- oyurd1mBd7pXVjbFtWF3Wn131uh7pwDJzRONynxBM7pAx9NKv2UgcRE5mFZ+4xrzFC5i 7, 35 -- wy7mWRKeuK1PCS2ntLRUi9ry7/HxcvKYJQ1No= 7, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 7, 35 -- :content-type:content-transfer-encoding; 7, 35 -- b=HlXhCjMYmz5BLqOAq5cXYutZVGco4ITzK83i2yKBFW7eKNhJivkmLkdwcY71FtwQZC 7, 35 -- D60fyp89pBLFaIJyCy4Di48Gl14illOKP3uCBvCQXrAjektUFEmQG9VGdPWOPnKJukbR 7, 35 -- GV/LXZ3SzbXaEsBK40RwEzLLOCcpxSK9vBOlI= 7, 35 -- MIME-Version: 1.0 7, 35 -- Received: by 10.227.182.68 with SMTP id cb4mr6280208wbb.218.1295389206945; 7, 35 -- Tue, 18 Jan 2011 14:20:06 -0800 (PST) 7, 35 -- Received: by 10.227.143.213 with HTTP; Tue, 18 Jan 2011 14:20:06 -0800 (PST) 7, 35 -- In-Reply-To: {201101132226.p0DMQEek018977-at-ns.microscopy.com} 7, 35 -- References: {201101132226.p0DMQEek018977-at-ns.microscopy.com} 7, 35 -- Date: Tue, 18 Jan 2011 17:20:06 -0500 7, 35 -- Message-ID: {AANLkTinRDV6AB=4g=G4YztWiMVtXapMc=fzVYz82QPFa-at-mail.gmail.com} 7, 35 -- Subject: Re: [Microscopy] RE: Ernst Ruska 7, 35 -- From: Joel Sheffield {joelsheffield-at-gmail.com} 7, 35 -- To: NEERAJG-at-clemson.edu, microscopy-at-microscopy.com 7, 35 -- Content-Type: text/plain; charset=ISO-8859-1 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0IMK7qv031430 ==============================End of - Headers==============================
Thanks to all who replied with offers for some Santovac 5. I possibly have a very local source, plus some other possibilities, so others who were thinking of helping out are off the hook. Promised sacrifices to the EM gods have already been offered. Just waiting now for the smoke to clear...
As usual, the Listserv is an invaluable go-to source for all sorts of goofy problems, esp. encountered in the wilds of the Great White North.
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Directions: That useless little piece of paper, containing incomprehensible language, usually written in Spanish and Japanese, that one refers to, only after botching the construction of the over-priced and (also usually) useless item that one found so necessary to own, only that very morning.
==============================Original Headers============================== 10, 19 -- From jehrman-at-mta.ca Wed Jan 19 07:17:06 2011 10, 19 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.114]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JDH5SO015384 10, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 07:17:06 -0600 10, 19 -- Received: from host-22-194.mta.ca ([138.73.22.194]:56716) 10, 19 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) 10, 19 -- (Exim 4.71) 10, 19 -- (envelope-from {jehrman-at-mta.ca} ) 10, 19 -- id 1PfXuJ-00014B-BJ 10, 19 -- for Microscopy-at-microscopy.com; Wed, 19 Jan 2011 09:17:03 -0400 10, 19 -- Message-ID: {4D36E44D.8010700-at-mta.ca} 10, 19 -- Date: Wed, 19 Jan 2011 09:17:01 -0400 10, 19 -- From: James Ehrman {jehrman-at-mta.ca} 10, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 10, 19 -- MIME-Version: 1.0 10, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 10, 19 -- Subject: Thanks! Re: Santovac 5? I need a few drops... 10, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I’ve been thinking about EDS line scans and I’m attempting to maximize the data I collect.
I run at 20 KV on iron samples so my electron interaction range is between 1.5 to 2.3um (Berthe or K-O range calculations). According to data provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um at 700x. So right away I see if each image pixel represents a data point, I’m sampling about 2 pixel volumes per data point. Looking for a sudden and sharp change in element concentration seem difficult, but wait there’s more. Let us introduce another plot complication.
My customers don’t want to scan the entire image, tooo much data…
So I scan a smaller section of image described above and instruct the computer to take 512 data points along a line that might only be 400 (visual estimate from screen) image pixels long.
So… Should I reduce the image resolution so that each image pixel will be one data point and scan the entire image? Would it be better to lower the magnification so the image pixels are larger (say 1.2um ). I could go to 100x and 512 horizontal image pixel so each image pixel is 2.3um?
I’m looking for slight changes in element concentration at grain boundaries and precipitates and of course I want to produce the most meaningful data possible. I’m running at conditions that suggest my electron interactive volume is larger than my spatial resolution as to produce “continuous” data.
Or am I just over thinking the process?
Any suggestion or comments would be welcome
Frank Lincoln Electric Company -- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 11, 17 -- From frank_karl-at-lincolnelectric.com Wed Jan 19 07:37:23 2011 11, 17 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JDbNoO031503 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 07:37:23 -0600 11, 17 -- Subject: EDS line scans and time on my hands 11, 17 -- To: Microscopy-at-microscopy.com 11, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 11, 17 -- Message-ID: {OF4A811DE5.D47D1408-ON8525781D.004A569D-8525781D.004AD372-at-lincolnelectric.com} 11, 17 -- From: Frank_Karl-at-lincolnelectric.com 11, 17 -- Date: Wed, 19 Jan 2011 08:38:03 -0500 11, 17 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 11, 17 -- 01/19/2011 08:37:18 AM 11, 17 -- MIME-Version: 1.0 11, 17 -- Content-Type: text/plain; 11, 17 -- charset="UTF-8" 11, 17 -- Content-Transfer-Encoding: 8bit 11, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p0JDbNoO031503 ==============================End of - Headers==============================
Join the LinkedIn Group at http://www.linkedin.com/groupRegistration?gid=3733425
And check out The Cell: An Image Library at www.cellimagelibrary.org.
David
David Orloff Manager, Image Library The American Society for Cell Biology 8120 Woodmont Avenue, Suite 750 Bethesda, MD 20814-2762 T: 301-347-9300/Direct: 301-347-9305 F: 301-347-9310 E-mail: dorloff-at-ascb.org Web site: www.ascb.org The Cell: An Image LibraryT: www.cellimagelibrary.org
==============================Original Headers============================== 9, 25 -- From DOrloff-at-ascb.org Wed Jan 19 08:25:59 2011 9, 25 -- Received: from smtp.ascb.org (coalitionforlifesciences.org [173.13.233.102] (may be forged)) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JEPxGk016078 9, 25 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 08:25:59 -0600 9, 25 -- Received: from ASCB2.ascb.local ([::1]) by ASCB2.ascb.local ([::1]) with mapi; 9, 25 -- Wed, 19 Jan 2011 09:25:57 -0500 9, 25 -- From: David Orloff {DOrloff-at-ascb.org} 9, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 25 -- CC: David Orloff {DOrloff-at-ascb.org} 9, 25 -- Date: Wed, 19 Jan 2011 09:25:57 -0500 9, 25 -- Subject: Cell Microscopy 9, 25 -- Thread-Topic: Cell Microscopy 9, 25 -- Thread-Index: Acu35EQpVdvSPTRcRiqphmn0f0HxFQAAGyHw 9, 25 -- Message-ID: {8D9C2A7731877D45B1691E78451EC3BE4C6BE08D-at-ASCB2.ascb.local} 9, 25 -- References: {8D9C2A7731877D45B1691E78451EC3BE4C6BE08C-at-ASCB2.ascb.local} 9, 25 -- In-Reply-To: {8D9C2A7731877D45B1691E78451EC3BE4C6BE08C-at-ASCB2.ascb.local} 9, 25 -- Accept-Language: en-US 9, 25 -- Content-Language: en-US 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- acceptlanguage: en-US 9, 25 -- Content-Type: text/plain; charset="iso-8859-1" 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JEPxGk016078 ==============================End of - Headers==============================
For those who may find themselves in my predicament, Bart has small quantities of Santovac 5 available:
Jim,
I have Santovac 5 in small glass vials. 30 drops for $10.
You might try just brushing out the lead screws with acetone and a paper towel guard to collect the spray. That will disperse any residual coagulated Santovac 5.
Though I've been on this list for almost its entire life, I can no longer post to the group since I can't get through the spam filter.
You might consider posting this e-mail to the group.
Bart Cannon Cannon Microprobe 1041 NE 100th Seattle, WA 98125 206 522 9233
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Directions: That useless little piece of paper, containing incomprehensible language, usually written in Spanish and Japanese, that one refers to, only after botching the construction of the over-priced and (also usually) useless item that one found so necessary to own, only that very morning.
==============================Original Headers============================== 13, 19 -- From jehrman-at-mta.ca Wed Jan 19 08:33:21 2011 13, 19 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.114]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JEXK0o028862 13, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 08:33:21 -0600 13, 19 -- Received: from host-22-194.mta.ca ([138.73.22.194]:56984) 13, 19 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) 13, 19 -- (Exim 4.71) 13, 19 -- (envelope-from {jehrman-at-mta.ca} ) 13, 19 -- id 1PfZ68-0002je-LA 13, 19 -- for Microscopy-at-microscopy.com; Wed, 19 Jan 2011 10:33:20 -0400 13, 19 -- Message-ID: {4D36F62E.3040106-at-mta.ca} 13, 19 -- Date: Wed, 19 Jan 2011 10:33:18 -0400 13, 19 -- From: James Ehrman {jehrman-at-mta.ca} 13, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 13, 19 -- MIME-Version: 1.0 13, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 13, 19 -- Subject: source of small amounts of Santovac 5 13, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are trying to revive a Fuji Pitctrography 3000. We have the SCSI to USB adapters but the pc we have still does not see that the printer is there. We are using a pc with the Vista opreating system (don't start with the comments about that!) and the Fuji uses Photoshop plug-ins.
Any suggestions as to what to try next to get this printer up and runing?
Thanks in advance.
Paula
-- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#M251 Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 7, 33 -- From patpxs-at-gmail.com Wed Jan 19 11:07:36 2011 7, 33 -- Received: from mail-px0-f169.google.com (mail-px0-f169.google.com [209.85.212.169]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JH7aga021466 7, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 11:07:36 -0600 7, 33 -- Received: by pxi12 with SMTP id 12so261253pxi.0 7, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 09:07:35 -0800 (PST) 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 33 -- d=gmail.com; s=gamma; 7, 33 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 7, 33 -- :content-type:content-transfer-encoding; 7, 33 -- bh=3/OjsPuFR29OGrQhvHgsM7IY/iVkuv9HWZjelQ5PVMk=; 7, 33 -- b=vkM9a1Iq07A3dwNe+ZhdWk7lbyi6iAKGVzzLBSEHijXr1goqQgi9KT6CYVxTFDNTTv 7, 33 -- G8JZahYAx5ATcMxhAx+FfSsr56GKlOn8coV5kk27iYDTrTmYMd4Yq4EhsPHmxcXlavr/ 7, 33 -- 59evQkwZmybdjYc4Q1dIJ4rj9vZ8O8aCCn294= 7, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 33 -- d=gmail.com; s=gamma; 7, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 7, 33 -- :content-transfer-encoding; 7, 33 -- b=b5ddZv8nZUF3LbBw7t5zTX7jiOZTmMOVg9PPNsolpkXN2N0GXBwFpL2N257Slrrpps 7, 33 -- lKYyKW/Q7fOowq295rBP0kPs/JNB+iprMg+9Ynk2QYWPxu0CXwEtDYudo6piSTV7NqVl 7, 33 -- QrW7oTdPSnOj6wvwG+6NTfLkpiKp07SMxJNYA= 7, 33 -- MIME-Version: 1.0 7, 33 -- Received: by 10.42.172.130 with SMTP id n2mr1164409icz.133.1295456855411; Wed, 7, 33 -- 19 Jan 2011 09:07:35 -0800 (PST) 7, 33 -- Received: by 10.42.219.68 with HTTP; Wed, 19 Jan 2011 09:07:35 -0800 (PST) 7, 33 -- Date: Wed, 19 Jan 2011 12:07:35 -0500 7, 33 -- Message-ID: {AANLkTikeTzxtk6s-pi+X4r7+xQ6zU=rQf37J3iR2X8rt-at-mail.gmail.com} 7, 33 -- Subject: Fuji Pictrography 3000 7, 33 -- From: Paula Sicurello {patpxs-at-gmail.com} 7, 33 -- To: Microscopy-at-microscopy.com 7, 33 -- Content-Type: text/plain; charset=ISO-8859-1 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JH7aga021466 ==============================End of - Headers==============================
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Title-Subject: [Filtered] How do I remove front panels on Hitachi TM-1000
Message: Hello All, I need to remove the front panels on the Hitachi TM-1000 Tabletop SEM. Has anyone done this? If so, please respond. This is not easy! I want to have the turbo molecular pump serviced. Thanks! Winnie Westbrook, M.Ed. Electron Microscopy Research Lab Virginia State University Petersburg, VA 23806 804-524-5659
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Email: anthonyribaudo3-at-gmail.com Name: Anthony Ribaudo
Organization: TRI Princeton
Title-Subject: [Filtered] SEM imaging of wax
Message: Can anyone suggest guidelines for imaging a paraffin wax coating on a metal or polymeric substrate via FESEM. The plan is to detect the wax layer that is thought to be several hundred nanometers thick? Are there any waxes that would be more stable to image under the electron beam than paraffin wax?
A postdoc/research associate position supported by a newly renewed 5-year NIH grant is available immediately in the Department of Biochemistry, NYU School of Medicine. The research of the position focuses on structural studies, using cryo-electron microscopy and electron tomography, of uro-epithelial apical membrane and its receptor function for uropathogenic E. coli (UPEC) in urinary tract infection (UTI) (see lab webpage: http://kong.med.nyu.edu). Experience in electron microscopy and image processing is desirable. Interested candidates should send CV and reference information to xiangpeng.kong-at-med.nyu.edu.
------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. =================================
==============================Original Headers============================== 5, 32 -- From Fengxia.Liang-at-med.nyu.edu Wed Jan 19 14:33:35 2011 5, 32 -- Received: from mail4.nyumc.org (mail4.nyumc.org [216.165.126.170]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JKXZf5014480 5, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 14:33:35 -0600 5, 32 -- X-IronPort-Anti-Spam-Filtered: true 5, 32 -- X-IronPort-Anti-Spam-Result: ApsEAEvZNk0KkyXk/2dsb2JhbAClO7cpiDWFUASEbw 5, 32 -- X-IronPort-AV: E=Sophos;i="4.60,345,1291611600"; 5, 32 -- d="scan'208";a="112262987" 5, 32 -- Received: from msgwsdcpht62.nyumc.org ([10.147.37.228]) 5, 32 -- by mail4.nyumc.org with SMTP; 19 Jan 2011 15:33:34 -0500 5, 32 -- Received: from MSGWSDCPMB05.nyumc.org ([10.147.54.52]) by 5, 32 -- MSGWSDCPHT62.nyumc.org ([10.147.37.228]) with mapi; Wed, 19 Jan 2011 15:33:35 5, 32 -- -0500 5, 32 -- From: "Liang, Fengxia" {Fengxia.Liang-at-med.nyu.edu} 5, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 32 -- Date: Wed, 19 Jan 2011 15:33:33 -0500 5, 32 -- Subject: Postdoc/research associate position available at NYU School 5, 32 -- ofMedicine 5, 32 -- Thread-Topic: Postdoc/research associate position available at NYU School 5, 32 -- ofMedicine 5, 32 -- Thread-Index: Acu4GCPT+mxRv30pMEaqNBbF070AyQ== 5, 32 -- Message-ID: {C95CB4CD.65DE%Fengxia.Liang-at-med.nyu.edu} 5, 32 -- Accept-Language: en-US 5, 32 -- Content-Language: en-US 5, 32 -- X-MS-Has-Attach: 5, 32 -- X-MS-TNEF-Correlator: 5, 32 -- user-agent: Microsoft-Entourage/13.4.0.100208 5, 32 -- acceptlanguage: en-US 5, 32 -- Content-Type: text/plain; charset="us-ascii" 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JKXZf5014480 ==============================End of - Headers==============================
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Email: z.zhou-at-lboro.ac.uk Name: Zhou
Organization: Dept of Materials, Loughborough University, UK
Title-Subject: [Filtered] TEM diffraction 3nm precipitates
Message: Greetings listers,
Iím studying crystal structure of some carbides/nitrides precipitates in steel. The precipitates are 2-5nm size in a C replica TEM specimen, V-, Cr-, and Nb-rich by STEM/EDX.
How can I get electron diffraction or crystal structure information of these fine precipitates?
I tried on a TecnaiF20 TEM using nanoprobe spot 7, focused on a small particle, then diffraction, most of the time I got amorphous rings of C support, no obvious diffraction discs. STEM mode did indicate some C contamination on the session. Assuming minimal C contamination, is it possible that this method can give me some sort of convergence beam electron diffraction pattern, which may help me determine the crystal structure of the precipitates?
Iím also open to other methods.
Your advice is highly appreciated.
Zhou
Dr Zhaoxia Zhou Dept of Materials Loughborough University Loughborough, UK LE11 3TU
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Email: sbarlow-at-sciences.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] color Laser printers
Message: Hello All
Our general lab printer, a monochrome HP 2100, is on its last legs and needs to be replaced, preferably with a color, duplex capable printer.
We are looking at the Ricoh C430dn or the HP CP4525dn. We use the lab printer for copy images, documentation of images. student notebooks, etc.
I have had lots of experience with the HP printers, but none with the Ricoh. Can anyone give me insights into their own experiences with these two printers or the companies? Any other recommendations for a laser printer?
We will be imaging a fairly large number of norovirus samples using negative staining. This is just for sample screening to document the presence or absence of the virus.
The samples could be sent in fresh but I thought it might be better to fix them prior to shipping so that they are not a health hazard. That might also allow us to store them unfrozen for an extended period of time (months) with reduced chance of deterioration or contamination.
Does anyone have a validated protocol that they are willing to share? I used to know someone at the CDC who did TEM but no longer have that contact.
Thanks, Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 7, 28 -- From dsherman-at-purdue.edu Wed Jan 19 15:36:50 2011 7, 28 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JLanJM026947 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 15:36:50 -0600 7, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 7, 28 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0JLan3m008331 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:36:49 -0500 7, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.110]) by WPPEXHUB01F.purdue.lcl 7, 28 -- ([::1]) with mapi; Wed, 19 Jan 2011 16:36:48 -0500 7, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 7, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 28 -- Date: Wed, 19 Jan 2011 16:36:46 -0500 7, 28 -- Subject: Protocol for Norovirus TEM 7, 28 -- Thread-Topic: Protocol for Norovirus TEM 7, 28 -- Thread-Index: Acu4IPihFFWUNBgITEScuEiQwrSisg== 7, 28 -- Message-ID: {C95CC39E.B10D%dsherman-at-purdue.edu} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 7, 28 -- acceptlanguage: en-US 7, 28 -- Content-Type: text/plain; charset="us-ascii" 7, 28 -- MIME-Version: 1.0 7, 28 -- X-PMX-Version: 5.5.9.388399 7, 28 -- X-PerlMx-Virus-Scanned: Yes 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JLanJM026947 ==============================End of - Headers==============================
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Email: abel.orellana-at-sydney.edu.au Name: Abel Orellana
Organization: University of Sydney
Title-Subject: [Filtered] University of Sydney - Career Opportunity
Message: GENERAL OFFICE ADMINISTRATOR THE AUSTRALIAN CENTRE FOR MICROSCOPY AND MICROANALYSIS (ACMM) REFERENCE NO. 2615 /0810
ď Be part of the largest Microscopy Facility of its type in Australia ď Opportunity to work on newly emerging microscopy techniques ď Remuneration package: $80,157
The University of Sydney is Australia's premier University with an outstanding global reputation for academic and research excellence, and employs over 6,800 permanent staff supporting over 46,000 students.
The Australian Centre for Microscopy and Microanalysis (ACMM) aims to provide leadership in the development of innovation and ingenuity in Australian science and engineering. The Centreís vision is to be a world-leading facility for modern microscopy and microanalysis, offering premier instruments, services and training to researchers from across Sydney and around Australia, and providing strong leadership in the national and international characterisation communities.
We are currently seeking an experienced Administrator to provide effective front desk and administrative support to the main office of the ACMM. Ideally suited to someone with an interest in science, this is an excellent opportunity to assist in the co-ordination of research administration actives within research services.
You will be responsible for the professional maintenance of the ACMM front desk, while being first point-of contact for all queries. As such it is imperative that you have excellent communication and problem solving skills. The position will see you co-ordinate and improve the ACMM administration procedures including but not limited to, arranging new user meetings; liaising with users and visitors to the Centre in terms of access; coordinating inductions for new staff and students and providing support to the maintenance of the Centreís website. Your demonstrated ability to adapt to change and willingness to undertake new tasks as the need arises will be essential.
We are looking for a committed individual who will thrive in a busy and changing environment. To succeed, you will have previous experience working in a busy office environment, existing high level IT skills including creation of spreadsheets, experience drafting and editing reports along with experience producing high level meeting minutes. You must also have strong development and project management skills and the ability to communicate with a diverse range of individuals. Previous experience in a Tertiary environment will be highly regarded.
The position is continuing subject to the completion of a satisfactory probation period for new appointees. Membership of a University approved superannuation scheme is a condition of employment for new appointees.
Remuneration package: a competitive remuneration package is available of $80,157 (consisting of a base salary $67,734, leave loading and up to 17% employerís contribution to superannuation).
All applications must be submitted via The University of Sydney careers website. Visit sydney.edu.au/positions and search by the reference number for more information and to apply.
CLOSING DATE: 31 January 2011
The University is an Equal Opportunity employer committed to equity, diversity and social inclusion. Applications from equity target groups and women are encouraged.
The University reserves the right not to proceed with any appointment.
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Email: abel.orellana-at-sydney.edu.au Name: Abel Orellana
Organization: University of Sydney
Title-Subject: [Filtered] University of Sydney - Career Opportunity
Message: SCANNING ELECTRON MICROSCOPY SPECIALIST THE AUSTRALIAN CENTRE FOR MICROSCOPY AND MICROANALYSIS (ACMM) REFERENCE NO. 4273 /1210
ď Be part of the largest Microscopy Facility of its type in Australia ď Opportunity to work on newly emerging microscopy techniques ď Remuneration package: $80,157
The University of Sydney is Australia's premier University with an outstanding global reputation for academic and research excellence, and employs over 6,800 permanent staff supporting over 46,000 students.
The Australian Centre for Microscopy and Microanalysis (ACMM) aims to provide leadership in the development of innovation and ingenuity in Australian science and engineering. The Centreís vision is to be a world-leading facility for modern microscopy and microanalysis, offering premier instruments, services and training to researchers from across Sydney and around Australia, and providing strong leadership in the national and international characterisation communities.
As the Scanning Electron Microscopy Specialist you will provide instruction, training and support to users of the Centre's analytical scanning electron microscope facilities including primarily the needs of users in specimen preparation, microscopy, microanalysis, image/data analysis and interpretation. You will have demonstrated expertise and skills in specimen preparation techniques relevant to scanning electron microscopy (SEM) and associated techniques and skills in one or more of the following: focussed ion beam milling (FIB) techniques; environmental SEM (ESEM), cryo-SEM imaging; In-Situ SEM instrumentation; Electron Backscattered Diffraction (EBSD); quantitative image analysis; X-ray microanalysis, X-ray mapping; Mineral phase mapping
To succeed, you will possess an Honours degree in science or engineering whereas a doctoral degree with a high SEM component will be highly advantageous. You will be a self motivated individual with exceptional scientific communication skills and a demonstrated ability to relate to students, users, academic and researchers at all levels. Experience in running and working in a multi-user laboratory and experience with the instruction of SEM techniques is desirable.
The position is full-time, fixed term for three years subject to the completion of a satisfactory probation period for new appointees with the possibility of further offer of employment. Membership of a University approved superannuation scheme is a condition of employment for new appointees. Visa sponsorship and relocation assistance may be offered to suitable overseas applicants. A wide range of employment benefits are also available, including Living Away From Home Allowance (LAFHA).
Remuneration package: a competitive remuneration package is available of $80,157 (consisting of a base salary $67,734, leave loading and up to 17% employerís contribution to superannuation).
All applications must be submitted via The University of Sydney careers website. Visit sydney.edu.au/positions and search by the reference number for more information and to apply.
CLOSING DATE: 31 January 2011
The University is an Equal Opportunity employer committed to equity, diversity and social inclusion. Applications from equity target groups and women are encouraged.
The University reserves the right not to proceed with any appointment.
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Email: a.o.orellana Name: Abel Orellana
Organization: University of Sydney
Title-Subject: [Filtered] University of Sydney - Career Opportunity
Message: MULTIMEDIA COMMUNICATIONS OFFICER AUSTRALIAN MICROSCOPY & MICROSCOPY RESEARCH FACILITY (AMMRF) REFERENCE NO. 4239 /1210
ď Collaborate with AMMRF partners ď Opportunity to work with a variety of Media Communications ď Remuneration package: $80,157 ď The University of Sydney is Australia's premier University with an outstanding global reputation for academic and research excellence, and employs over 6,800 permanent staff supporting over 46,000 students.
The ACMM provides leadership in the development and application of advanced microscopy and microanalysis techniques that support innovation in Australian science and engineering. The centre incorporates a substantial research portfolio, is a node of the ARC Centre if Excellence for Design in Light Metals and is the headquarters for the AMMRF, The AMMRF is Australiaís national research facility for the characterisation of materials through macro, meso, nano and atomic length scales by means of advanced microscopy and microanalysis. The facility is a joint venture of eight universities, which forms a grid of microscopy facilities with strategic links to six other laboratories throughout Australia.
As the Multimedia Communications Officer you will collaborate with AMMRF partners as well as teams within the University of Sydney, to facilitate and produce high-quality communications and promotional outcomes. This will include the creation, design and production of relevant materials utilising a variety of media such as written documents, presentations and the AMMRF and ACMM websites. You will also assist in the organisation and management of major events and brand implementation for the AMMRF and the ACMM.
To be successful you must have previous experience in the production of high quality print and digital communications as well as the ability to draft, edit and analyse print for reports, publications and presentations. Your experience and knowledge working with web authoring, web design and graphic design software as well your exceptional working knowledge of Adobe Creative Suite Premium and Microsoft Office is essential, as is the ability to build and maintain effective working relationships. Previous experience working in a scientific organisation will be highly regarded.
The position is for 2.5 years, fixed term subject to the completion of a satisfactory probation period for new appointees with the possibility of further offer of employment. Membership of a University approved superannuation scheme is a condition of employment for new appointees.
Remuneration package: a competitive remuneration package is available of $80,157 (consisting of a base salary $67,734 leave loading and up to 17% employerís contribution to superannuation).
All applications must be submitted via The University of Sydney careers website. Visit sydney.edu.au/positions and search by the reference number for more information and to apply.
CLOSING DATE: 31 January 2011
The University is an Equal Opportunity employer committed to equity, diversity and social inclusion. Applications from equity target groups and women are encouraged.
Appointment is on merit; as women are under-represented at this employment level suitably qualified women are encouraged to apply.
The University reserves the right not to proceed with any appointment.
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Email: rfoley-at-uab.edu Name: Robin Foley
Organization: UAB
Title-Subject: [Filtered] Environmental SEM for Biological Work
Message: Is there heavy use of ESEM for biological SEM? What is commonly done on biological ESEM samples? We're looking at purchasing an environmental SEM and the sales rep. tells us we will need a Peltier stage to cool the sample to look at wet samples. Unfortunately, the maximum sample size for the Peltier stage is 3 mm across. This seems tiny to me for an SEM. Are there many biological ESEM applications that don't require the sample to be wet?
Thanks,
Robin Foley (who spends most of her SEM time looking at metals, ceramics and polymers!)
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Email: David.Llewellyn-at-anu.edu.au Name: David Llewellyn
Message: Am after some information re the Tenupol-2 and associated Polypower Power Supply best of all would be off-line with somebody who has one of these, thanks, David.
*Post-Doctoral Research Associate Position in Aberration-Corrected STEM*
* *
A post-doctoral research associate position is available in the Nanoscale Physics Group at the University of Illinois at Chicago to work on the application of atomic-resolution Z-contrast and annular bright-field imaging, as well as electron energy-loss spectroscopy (EELS). The successful candidate will work with our new JEOL ARM200CF, an aberration-corrected cold-field emission STEM with sub-Ĺ and sub-eV resolution, equipped with several in-situ holders.
Candidates should have a Ph.D. in Physics, Materials Science or related disciplines. The position requires extensive experience in transmission electron microscopy and electron energy-loss spectroscopy. Experience working with aberration-corrected scanning transmission electron microscopy is also preferred.
This postdoctoral position is available immediately, will be renewable on an annual basis, and is anticipated for at least two years. The position is open until it is filled.
Interested candidates can apply by email by sending a cover letter, CV with a complete list of publications, and contact information of 3 professional references to:
Professor Robert F Klie
Department of Physics
University of Illinois at Chicago
845 W Taylor Street, M/C 273
Chicago, IL 60607
email: rfklie-at-uic.edu
-- Robert F. Klie, PhD Assistant Professor University of Illinois at Chicago Department of Physics Chicago, IL 60607 Tel: 312-996-6064 Fax: 312-996-9016
Editor Journal of Undergraduate Research Website {http://jur.phy.uic.edu}
President Midwest Microscopy and Microanalysis Society (M^3 S) Website {http://midwestmicroscopy.org}
==============================Original Headers============================== 15, 18 -- From rfklie-at-uic.edu Wed Jan 19 17:18:47 2011 15, 18 -- Received: from mail-4.cc.uic.edu (mail-4.cc.uic.edu [128.248.155.184]) 15, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JNIlo0025624 15, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 17:18:47 -0600 15, 18 -- Received: from [131.193.192.91] (klielaptop.phy.uic.edu [131.193.192.91]) 15, 18 -- (authenticated bits=0) 15, 18 -- by mail-4.cc.uic.edu (8.14.4/8.14.4) with ESMTP id p0JNIlFP021499 15, 18 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 15, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 17:18:47 -0600 15, 18 -- Message-ID: {4D377152.4010000-at-uic.edu} 15, 18 -- Date: Wed, 19 Jan 2011 17:18:42 -0600 15, 18 -- From: Robert Klie {rfklie-at-uic.edu} 15, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 15, 18 -- MIME-Version: 1.0 15, 18 -- To: Microscopy-at-microscopy.com 15, 18 -- Subject: STEM post-doc position at UIC 15, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
It is certainly clear that there are many interesting stories --some urban legends, and some insights into the minds of those that founded the discipline of thin section electron microscopy. During the mid '70's, I taught an EM course at Rutgers, Camden. In the basement, along with an RCA EMU-2 (!) was a thermal advance microtome that I was told was developed by Sjostrand. This thing consisted of a disk-like plate, about 4-5 inches in diameter, with an eccentrically mounted arm that stuck out of the plate. The arm had a chuck for a tissue sample, and a heating coil. The plate itself was mounted in a frame and lubricated with oil --the plate acted like a large oil bearing. The plate was linked to a motor on the wall by a long V belt (to minimize vibration), and the entire plate rotated, bringing the block past the knife on each rotation. The sections that resulted tended to be arc shaped. We got it working, but it was a terrifying thing to see. I wonder if anyone in this group remembers this type of machine --assuming that my description is comprehensible.
The other microtome that I used, and really admire, was the Huxley microtome, originally sold by Cambridge, and ultimately marketed by LKB. Although it used a mechanical advance, all of the movements of the cutting arm were made by bending sheets of spring steel, so that there were no surfaces that moved against each other. Moreover, the cutting stroke was controlled by an oil-filled damper, to minimize chatter. --a remarkable and stable machine.
Now, the urban legend. I had heard that a group (unnamed) was having a vigorous discussion about the best way to strop a steel knife so as to get the best edge for cutting thin sections. How many strokes, in which direction, which side of the blade, etc. During the discussion, a bottle of Coke was knocked off the table and broke into many pieces. The story goes that one, or another, of these people took up one of the shards, and suggested that it would make a good knife edge. Verification anyone?
Joel
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
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This urban legend differs slightly according to the origin of the narrator:
An american talks about a bottle of coke. A french talks about a bottle of ricard A belgian talks about a flask of beer An italian talks about a bottle of wine A german talks about a bottle of schnaps A russian talks about a bottle of vodka. A japanese talks about a bottle of sake
Actually it would be interesting to test all of them to see which one cuts better. Now you just need convincing words for your grant....
Stephane
----- Original Message ---- X-from: "joelsheffield-at-gmail.com" {joelsheffield-at-gmail.com} To: nizets2-at-yahoo.com Sent: Thu, January 20, 2011 6:51:55 AM
It is certainly clear that there are many interesting stories --some urban legends, and some insights into the minds of those that founded the discipline of thin section electron microscopy. During the mid '70's, I taught an EM course at Rutgers, Camden. In the basement, along with an RCA EMU-2 (!) was a thermal advance microtome that I was told was developed by Sjostrand. This thing consisted of a disk-like plate, about 4-5 inches in diameter, with an eccentrically mounted arm that stuck out of the plate. The arm had a chuck for a tissue sample, and a heating coil. The plate itself was mounted in a frame and lubricated with oil --the plate acted like a large oil bearing. The plate was linked to a motor on the wall by a long V belt (to minimize vibration), and the entire plate rotated, bringing the block past the knife on each rotation. The sections that resulted tended to be arc shaped. We got it working, but it was a terrifying thing to see. I wonder if anyone in this group remembers this type of machine --assuming that my description is comprehensible.
The other microtome that I used, and really admire, was the Huxley microtome, originally sold by Cambridge, and ultimately marketed by LKB. Although it used a mechanical advance, all of the movements of the cutting arm were made by bending sheets of spring steel, so that there were no surfaces that moved against each other. Moreover, the cutting stroke was controlled by an oil-filled damper, to minimize chatter. --a remarkable and stable machine.
Now, the urban legend. I had heard that a group (unnamed) was having a vigorous discussion about the best way to strop a steel knife so as to get the best edge for cutting thin sections. How many strokes, in which direction, which side of the blade, etc. During the discussion, a bottle of Coke was knocked off the table and broke into many pieces. The story goes that one, or another, of these people took up one of the shards, and suggested that it would make a good knife edge. Verification anyone?
Joel
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
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Von: Muß Wolfgang Gesendet: Donnerstag, 20. Jänner 2011 11:46 An: 'joelsheffield-at-gmail.com' Cc: 'microscopy-at-microscopy.com' Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska"
Joel wrote: } Now, the urban legend. I had heard that a group (unnamed) was having a vigorous discussion about the best way to strop a steel knife so as to get the best edge for cutting thin sections. How many strokes, in which direction, which side of the blade, etc. During the discussion, a bottle of Coke was knocked off the table and broke into many pieces. The story goes that one, or another, of these people took up one of the shards, and suggested that it would make a good knife edge. Verification anyone? {
Joel
Good morning, Dear Joel, dear all
I can't tell or verify the above mentioned "Coke-shard-story" but - indeed - I can add information as to the "knocked off table"-part....
I had always heared (personal informations from elder REICHERT-freaks and service people, from lectures/lecturers in the 70ies and 80ies as well as personally from H. Sitte himself) that the group around H. SITTE at REICHERT (now LEICA) in the late 1950ies/60ies was/were doing such experiments with {glass plates} .... which were thrown down to the floor... and one of them (it was Sitte himself, as he told me) picked up the shards evaluating the edges of "the most promising one" for sectioning purposes (who of that group had the "invention" to use glass as a} crude { knife (element) I unfortunately don't know.
By the way, digging a little bit on this in Google, I found one hint on a document in a German data bank at ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.
{Ein einfaches Ultramikrotom mit thermischem Vorschub} veröffentlicht ("A simple ultramicrotome with thermal advance" published) in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367
Note: {Naturwissenschaften} is the former title of Biomedical and Life Sciences (SPRINGER) Another source: http://www.freepatentsonline.com/3828641.html: United States Patent US3828641:
==} Apparatus for adjusting the elevation of a specimen in microtomes, particularly ultramicrotomes Inventor: Hellmuth Sitte, HOMBURG (not Humburg [as written in the patent document!]/Saar Germany) Assignee: C.Reichert Optische Werke AG, Vienna, Austria) Filed: Nov. 10,1972
Also see http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false (From the book Michael J. Dykstra, Laura E. Reuss - 2003 (see page "History: in the text displayed the Reichert OMU-1 (before 1964, perhaps see document above in {Naturwissenschaften} 1955) from Sitte described as having thermal advance, Sitte was never happy with, since 1964: OMU-2 with thermal advance....
This just for your pleasure.... (and I sure that searching for Sitte Hellmuth or Sitte and microtome will find some results more on that interesting and exciting "history" of ultramicrotomy advances.....
Best wishes and regards, have a good and successful rest of the week and a beautiful weekend,
Wolfgang MUSS (Ph.D.) EM-Lab Gen.Hosp. SALK-LKH SALZBURG AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } Gesendet: Donnerstag, 20. Jänner 2011 06:51 } An: Muß Wolfgang } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } It is certainly clear that there are many interesting stories --some } urban legends, and some insights into the minds of those that founded } the discipline of thin section electron microscopy. } During the mid '70's, I taught an EM course at Rutgers, Camden. } In the basement, along with an RCA EMU-2 (!) was a thermal advance } microtome that I was told was developed by Sjostrand. } This thing consisted of a disk-like plate, about 4-5 inches in } diameter, with an eccentrically mounted arm that stuck out of the } plate. The arm had a chuck for a tissue sample, and a heating coil. } The plate itself was mounted in a frame and lubricated with oil --the } plate acted like a large oil bearing. The plate was linked to a motor } on the wall by a long V belt (to minimize vibration), and the entire } plate rotated, bringing the block past the knife on each rotation. The } sections that resulted tended to be arc shaped. We got it working, but } it was a terrifying thing to see. I wonder if anyone in this group } remembers this type of machine -- assuming that my description is } comprehensible. } } The other microtome that I used, and really admire, was the Huxley } microtome, originally sold by Cambridge, and ultimately marketed by } LKB. Although it used a mechanical advance, all of the movements of } the cutting arm were made by bending sheets of spring steel, so that } there were no surfaces that moved against each other. Moreover, the } cutting stroke was controlled by an oil-filled damper, to minimize } chatter. -- a remarkable and stable machine. } } Now, the urban legend. } I had heard that a group (unnamed) was having a vigorous discussion } about the best way to strop a steel knife so as to get the best edge } for cutting thin sections. How many strokes, in which direction, which } side of the blade, etc. During the discussion, a bottle of Coke was } knocked off the table and broke into many pieces. } The story goes that one, or another, of these people took up one of the } shards, and suggested that it would make a good knife edge. } Verification anyone? } } Joel } } -- } Joel B. Sheffield, Ph.D. } Biology Department, Temple University } 1900 North 12th Street } Philadelphia, PA 19122 } jbs-at-temple.edu } (215) 204 8839, fax (215) 204 0486 } http://astro.temple.edu/~jbs } } ==============================Original } Headers============================== } 5, 30 -- From joelsheffield-at-gmail.com Wed Jan 19 23:47:26 2011 } 5, 30 -- Received: from mail-ww0-f49.google.com (mail-ww0- } f49.google.com [74.125.82.49]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p0K5lQgB018304 } 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 23:47:26 } -0600 } 5, 30 -- Received: by wwb17 with SMTP id 17so256663wwb.18 } 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 } 21:47:25 -0800 (PST) } 5, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 30 -- d=gmail.com; s=gamma; } 5, 30 -- h=domainkey-signature:mime-version:date:message- } id:subject:from:to } 5, 30 -- :content-type; } 5, 30 -- bh=kPUiuV1UxIO0H1pRVf1DSBLH7XQoy5aO2bN7+G+szxM=; } 5, 30 -- } b=UXJCg0QqUwPbZUaTOkLx3i+Tq22ZLycH3163aMjr/YaOTUlNXA+V3Uah88ZvAbPim3 } 5, 30 -- } F7lWxUBBph/7Gqa/wiZDTEikXy4U+eCZ5a2FDRoLqH2T23rZ0bmrhTcekQ2FpBKKwEtC } 5, 30 -- cMlEC0ztRmc2ZtJPpd93Zra4IQSLR9zEbBxYE= } 5, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 5, 30 -- d=gmail.com; s=gamma; } 5, 30 -- h=mime-version:date:message- } id:subject:from:to:content-type; } 5, 30 -- } b=rwKrTeNXOA45KaZiFgYiUR8hEDH/WD4BYLEe+mTGsnjAUl+zABKfV824sTmyEd7EwU } 5, 30 -- } FeXrP78Ws/O0V/Tvzil7+UlEWx3u0y+u9UG3QRXhbJtGDNRwFUpTop9td+FSdKQ8ZDmD } 5, 30 -- a/L+pGrqTp0BPxBRSJFCfG/idbLcFzFb4b6fg= } 5, 30 -- MIME-Version: 1.0 } 5, 30 -- Received: by 10.227.155.66 with SMTP id } r2mr1805845wbw.73.1295502444180; Wed, } 5, 30 -- 19 Jan 2011 21:47:24 -0800 (PST) } 5, 30 -- Received: by 10.227.143.213 with HTTP; Wed, 19 Jan 2011 } 21:47:24 -0800 (PST) } 5, 30 -- Date: Thu, 20 Jan 2011 00:47:24 -0500 } 5, 30 -- Message-ID: } {AANLkTikoNq0MQhwanuX3FQwAJFQB7tscWZmu_MivkGTi-at-mail.gmail.com} } 5, 30 -- Subject: Microtome History - was "Ernst Ruska" } 5, 30 -- From: Joel Sheffield {joelsheffield-at-gmail.com} } 5, 30 -- To: microscopy-at-microscopy.com } 5, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - } Headers==============================
==============================Original Headers============================== 26, 35 -- From W.Muss-at-salk.at Thu Jan 20 04:47:01 2011 26, 35 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 26, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KAl0Sn024668 26, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 04:47:00 -0600 26, 35 -- Received: from localhost (localhost [127.0.0.1]) 26, 35 -- by hermes.salk.at (Postfix) with ESMTP id 7BC3AC386B 26, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:46:59 +0100 (CET) 26, 35 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 26, 35 -- Received: from hermes.salk.at ([127.0.0.1]) 26, 35 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 26, 35 -- with ESMTP id u1-3Xessr7tn for {microscopy-at-microscopy.com} ; 26, 35 -- Thu, 20 Jan 2011 11:46:59 +0100 (CET) 26, 35 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 26, 35 -- by hermes.salk.at (Postfix) with ESMTP id 0E565C3844 26, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:46:59 +0100 (CET) 26, 35 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.4675); 26, 35 -- Thu, 20 Jan 2011 11:46:58 +0100 26, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 35 -- Content-class: urn:content-classes:message 26, 35 -- MIME-Version: 1.0 26, 35 -- Content-Type: text/plain; 26, 35 -- charset="iso-8859-1" 26, 35 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" 26, 35 -- Date: Thu, 20 Jan 2011 11:46:58 +0100 26, 35 -- Message-ID: {06B4ED29F824524E98E8AA5BB640706293966C-at-N1RZ116.lksdom21.lks.local} 26, 35 -- X-MS-Has-Attach: 26, 35 -- X-MS-TNEF-Correlator: 26, 35 -- Thread-Topic: [Microscopy] Re: Microtome History - was "Ernst Ruska" 26, 35 -- Thread-Index: Acu4Zgs06778XDVBTg2CGrAzFhN94wAH0u6QAAJ7SGA= 26, 35 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 26, 35 -- To: {microscopy-at-microscopy.com} 26, 35 -- X-OriginalArrivalTime: 20 Jan 2011 10:46:58.0664 (UTC) FILETIME=[5CC80E80:01CBB88F] 26, 35 -- X-Scanned-By: SALK-Content-Filter 26, 35 -- Content-Transfer-Encoding: 8bit 26, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KAl0Sn024668 ==============================End of - Headers==============================
Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter.
You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe.
Does anyone know if this means the sample can be any size one likes?
Dave
-----Original Message----- X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu] Sent: 19 January 2011 22:53 To: David Patton
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Email: rfoley-at-uab.edu Name: Robin Foley
Organization: UAB
Title-Subject: [Filtered] Environmental SEM for Biological Work
Message: Is there heavy use of ESEM for biological SEM? What is commonly done on biological ESEM samples? We're looking at purchasing an environmental SEM and the sales rep. tells us we will need a Peltier stage to cool the sample to look at wet samples. Unfortunately, the maximum sample size for the Peltier stage is 3 mm across. This seems tiny to me for an SEM. Are there many biological ESEM applications that don't require the sample to be wet?
Thanks,
Robin Foley (who spends most of her SEM time looking at metals, ceramics and polymers!)
I was taught decades ago that early ultrathin sections were made in the following manner:
A sheet of plate glass was dropped on the floor. The shard with the best edge was selected and attached to the blade of a small window fan. The resin-embedded specimen was attached to the tip of a soldering iron. once everything was clamped and aligned, the fan was turned on, the soldering iron was plugged in, and the section were collected in a large pan of water held beneath the fan. (As the soldering iron heated, the thermal expansion advanced the specimen into the glass blade.) Sections of optimal thickness were selected from those floating in the pan on the basis of the color refracted.
I love the story. Does anyone know whether this even approaches a true story?
Thanks,
Don
P.S. Anyone still alive who has first-hand knowledge of this story would be pretty old by now, but I thought that I would give this a shot.
--
Donald L. Lovett e-mail: lovett-at-tcnj.edu Professor and Chairperson phone: 609-771-2876 Department of Biology fax: 609-637-5118 The College of New Jersey P.O. Box 7718 Ewing, NJ 08628-0718
==============================Original Headers============================== 10, 28 -- From lovett-at-tcnj.edu Thu Jan 20 08:19:04 2011 10, 28 -- Received: from mailgate-too.TCNJ.EDU (mailgate-too.TCNJ.EDU [159.91.13.67]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KEJ4dB030331 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:19:04 -0600 10, 28 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209]) 10, 28 -- by mailgate-too.TCNJ.EDU (Postfix) with ESMTP id 58885FF57E 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:13:11 -0500 (EST) 10, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id CAF513373AB 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU 10, 28 -- Received: from zcs.tcnj.edu ([127.0.0.1]) 10, 28 -- by localhost (zcs.tcnj.edu [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id fQjpUdFveSTN for {Microscopy-at-microscopy.com} ; 10, 28 -- Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Received: from [159.91.213.72] (unknown [159.91.213.72]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id AF779337393 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Message-ID: {4D384456.6040204-at-tcnj.edu} 10, 28 -- Date: Thu, 20 Jan 2011 09:19:02 -0500 10, 28 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu} 10, 28 -- Organization: Department of Biology, The College of New Jersey 10, 28 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 10, 28 -- MIME-Version: 1.0 10, 28 -- To: Microscopy-at-microscopy.com 10, 28 -- Subject: Early microtomy--urban legend? 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Anthony, If you don't have a cold stage on the FESEM, I would suggest finding some faithful old tungsten SEM that can stand the abuse of wax evaporating under the beam. That will also go a long way towards staying in the good graces of whoever is in charge of the FESEM.
If you have a cold stage, then others on the list can probably give you some help on that score.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message-----
X-from: anthonyribaudo3-at-gmail.com [mailto:anthonyribaudo3-at-gmail.com] Sent: Wednesday, January 19, 2011 3:36 PM To: kenconverse-at-qualityimages.biz
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Email: anthonyribaudo3-at-gmail.com Name: Anthony Ribaudo
Organization: TRI Princeton
Title-Subject: [Filtered] SEM imaging of wax
Message: Can anyone suggest guidelines for imaging a paraffin wax coating on a metal or polymeric substrate via FESEM. The plan is to detect the wax layer that is thought to be several hundred nanometers thick? Are there any waxes that would be more stable to image under the electron beam than paraffin wax?
I have heard all the microtome and glass knives stories also but have no evidence to their veracity. But I once was talking to Sanford Palay - an early pioneer in EM - and he told me how they used a rubber hammer to tap the outside of the TEM column to try and nudge the aperture into place. I can't imagine how tedious that must have been. Of course, the reward the pioneers all got from primitive microtomy and TEMs was that they got to discover amazing new things of a bigger magnitude than most of the incremental advances we all get to contribute to these days. But I think I would rather have done science then rather than now when high cost forces specialization and a greater proportion of time is devoted to finding funding rather than organelles. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu] Sent: Thursday, January 20, 2011 8:20 AM To: Phillips, Thomas E.
Fellow microscopists:
I was taught decades ago that early ultrathin sections were made in the following manner:
A sheet of plate glass was dropped on the floor. The shard with the best edge was selected and attached to the blade of a small window fan. The resin-embedded specimen was attached to the tip of a soldering iron. once everything was clamped and aligned, the fan was turned on, the soldering iron was plugged in, and the section were collected in a large pan of water held beneath the fan. (As the soldering iron heated, the thermal expansion advanced the specimen into the glass blade.) Sections of optimal thickness were selected from those floating in the pan on the basis of the color refracted.
I love the story. Does anyone know whether this even approaches a true story?
Thanks,
Don
P.S. Anyone still alive who has first-hand knowledge of this story would be pretty old by now, but I thought that I would give this a shot.
--
Donald L. Lovett e-mail: lovett-at-tcnj.edu Professor and Chairperson phone: 609-771-2876 Department of Biology fax: 609-637-5118 The College of New Jersey P.O. Box 7718 Ewing, NJ 08628-0718
==============================Original Headers============================== 10, 28 -- From lovett-at-tcnj.edu Thu Jan 20 08:19:04 2011 10, 28 -- Received: from mailgate-too.TCNJ.EDU (mailgate-too.TCNJ.EDU [159.91.13.67]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KEJ4dB030331 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:19:04 -0600 10, 28 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209]) 10, 28 -- by mailgate-too.TCNJ.EDU (Postfix) with ESMTP id 58885FF57E 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:13:11 -0500 (EST) 10, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id CAF513373AB 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU 10, 28 -- Received: from zcs.tcnj.edu ([127.0.0.1]) 10, 28 -- by localhost (zcs.tcnj.edu [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id fQjpUdFveSTN for {Microscopy-at-microscopy.com} ; 10, 28 -- Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Received: from [159.91.213.72] (unknown [159.91.213.72]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id AF779337393 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Message-ID: {4D384456.6040204-at-tcnj.edu} 10, 28 -- Date: Thu, 20 Jan 2011 09:19:02 -0500 10, 28 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu} 10, 28 -- Organization: Department of Biology, The College of New Jersey 10, 28 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 10, 28 -- MIME-Version: 1.0 10, 28 -- To: Microscopy-at-microscopy.com 10, 28 -- Subject: Early microtomy--urban legend? 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 32 -- From PhillipsT-at-missouri.edu Thu Jan 20 08:54:00 2011 19, 32 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KErxrG030207 19, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:54:00 -0600 19, 32 -- X-IronPort-Anti-Spam-Filtered: true 19, 32 -- X-IronPort-Anti-Spam-Result: AvsEAN7aN03RauUo/2dsb2JhbACkUXO1cYhEAoMQgj4EhG+BN4Qsg3Y 19, 32 -- Received: from um-tsmtpout1.um.umsystem.edu ([209.106.229.40]) 19, 32 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Jan 2011 08:53:59 -0600 19, 32 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 19, 32 -- Thu, 20 Jan 2011 08:53:59 -0600 19, 32 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 19, 32 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Thu, 20 Jan 2011 19, 32 -- 08:53:58 -0600 19, 32 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 19, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 32 -- Date: Thu, 20 Jan 2011 08:53:54 -0600 19, 32 -- Subject: RE: [Microscopy] Early microtomy--urban legend? 19, 32 -- Thread-Topic: [Microscopy] Early microtomy--urban legend? 19, 32 -- Thread-Index: Acu4rTCfGg2oH1rLRqikoaKr1g1gsgAA+QUQ 19, 32 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF1C4596EF1A-at-UM-EMAIL05.um.umsystem.edu} 19, 32 -- References: {201101201420.p0KEKJfH031321-at-ns.microscopy.com} 19, 32 -- In-Reply-To: {201101201420.p0KEKJfH031321-at-ns.microscopy.com} 19, 32 -- Accept-Language: en-US 19, 32 -- Content-Language: en-US 19, 32 -- X-MS-Has-Attach: 19, 32 -- X-MS-TNEF-Correlator: 19, 32 -- acceptlanguage: en-US 19, 32 -- Content-Type: text/plain; charset="us-ascii" 19, 32 -- MIME-Version: 1.0 19, 32 -- X-OriginalArrivalTime: 20 Jan 2011 14:53:59.0017 (UTC) FILETIME=[DE667590:01CBB8B1] 19, 32 -- Content-Transfer-Encoding: 8bit 19, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KErxrG030207 ==============================End of - Headers==============================
The University of Florida, College of Medicine, Department of Biochemistry and Molecular Biology seeks an enthusiastic Ph.D. level structural biologist, with experience in the area of cryo-electron microscopy and/or X-ray crystallography and image reconstruction to work on the structure of ssDNA virus proteins and intact capsids. The position will require knowledge of molecular biology, biochemistry, and structural biology approaches. Training can be provided, as needed, but a basic knowledge of the technical aspects of these approaches is required. In addition, projects in the lab often require collaborative effort, thus an ability/desire to work in a group setting is preferable. Salary will be negotiable and commensurate with experience. Interested applicants should send their Curriculum Vitae plus the names of three individuals who can provide a letter of reference to Dr. Mavis Agbandje-McKenna, Department of Biochemistry and Molecular Biology, 1600 SW Archer Road, PO Box 100245, Gainesville, FL 32610-0245 or email to mckenna-at-ufl.edu . The University of Florida is an Equal Opportunity Employee.
Thanks,
Byung-Ho Kang, Ph.D. Assistant Professor, Microbiology and Cell Science Director, Electron Microscopy and Bioimaging Lab, Interdisciplinary Center for Biotechnology Research University of Florida Gainesville, FL 32611 Tel: 352-846-0952 Fax: 352-392-5922
==============================Original Headers============================== 6, 26 -- From bkang-at-ufl.edu Thu Jan 20 08:54:46 2011 6, 26 -- Received: from smtp.ufl.edu (smtp03.osg.ufl.edu [128.227.74.70]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KEsj1f031129 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:54:45 -0600 6, 26 -- Received: from [10.249.16.125] ([10.249.16.125]) 6, 26 -- (authenticated bits=0) 6, 26 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id p0KEshlY017413 6, 26 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:54:44 -0500 6, 26 -- From: Byung-Ho Kang {bkang-at-ufl.edu} 6, 26 -- Content-Type: text/plain; charset=us-ascii 6, 26 -- Subject: Postdoctoral Research Associate Position available at the University of Florida 6, 26 -- Date: Thu, 20 Jan 2011 09:54:43 -0500 6, 26 -- Message-Id: {AF086E19-9824-4374-ACB7-4992258BA3D1-at-ufl.edu} 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Mime-Version: 1.0 (Apple Message framework v1082) 6, 26 -- X-Mailer: Apple Mail (2.1082) 6, 26 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 6, 26 -- definitions=2011-01-20_04:2011-01-20,2011-01-20,1970-01-01 signatures=0 6, 26 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=1 6, 26 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 6, 26 -- engine=6.0.2-1012030000 definitions=main-1101200053 6, 26 -- X-Spam-Level: * 6, 26 -- X-UFL-Spam-Level: * 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KEsj1f031129 ==============================End of - Headers==============================
Just a question disguised in an answer: wouldn't it be the right application to make a replica?
Stephane
----- Original Message ---- X-from: "anthonyribaudo3-at-gmail.com" {anthonyribaudo3-at-gmail.com} To: nizets2-at-yahoo.com Sent: Wed, January 19, 2011 9:39:49 PM
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Email: anthonyribaudo3-at-gmail.com Name: Anthony Ribaudo
Organization: TRI Princeton
Title-Subject: [Filtered] SEM imaging of wax
Message: Can anyone suggest guidelines for imaging a paraffin wax coating on a metal or polymeric substrate via FESEM. The plan is to detect the wax layer that is thought to be several hundred nanometers thick? Are there any waxes that would be more stable to image under the electron beam than paraffin wax?
Forgot how much fun these discussions of the "old days" can be. At Washington University there was an old Seimens microscope. At the time I was training as a tech, and using the microscope was not an option. However, the man who trained me, Charles Kuhn, told me that one of the microscopes he had used, and I believe he was referring to the old Seimens at WU, had to be aligned using a rubber mallet. Never did it personally, but was regaled with stories by one who did.
paul -- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 3, 21 -- From paul_hazelton-at-umanitoba.ca Thu Jan 20 09:36:45 2011 3, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFaitU013986 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:36:44 -0600 3, 21 -- Received: from [140.193.25.116] (basic116.medmb.umanitoba.ca [140.193.25.116]) 3, 21 -- (user=hazeltn mech=CRAM-MD5 bits=0) 3, 21 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0KFahNr022457 3, 21 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO); 3, 21 -- Thu, 20 Jan 2011 09:36:43 -0600 (CST) 3, 21 -- Message-ID: {4D38568A.2050009-at-umanitoba.ca} 3, 21 -- Date: Thu, 20 Jan 2011 09:36:42 -0600 3, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 3, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 3, 21 -- MIME-Version: 1.0 3, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 21 -- Subject: Re: [Microscopy] RE: Early microtomy--urban legend? 3, 21 -- References: {201101201456.p0KEu0Bl002900-at-ns.microscopy.com} 3, 21 -- In-Reply-To: {201101201456.p0KEu0Bl002900-at-ns.microscopy.com} 3, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 21 -- Content-Transfer-Encoding: 7bit 3, 21 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
I know at least 2 old microscopists who made glass knifes by breaking window glass - one of them here at CMU. He went to construction sites to get the broken windows.
I'm running down the reference and (I hope) an image, but one of the early tries at ultramicrotomy was sticking razor blades on a centrifuge rotor, then mount the sections on the tub, close the lid and turn on the centrifuge. The sections where then picked up from inside the tub, having been flung willy-nilly around the inside. (The image is used in the microtomy lecture to convince the students thin sectioning could be a lot worse than they think it is.)
Phil
} Von: Muţ Wolfgang } Gesendet: Donnerstag, 20. J”nner 2011 11:46 } An: 'joelsheffield-at-gmail.com' } Cc: 'microscopy-at-microscopy.com' } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } Joel wrote: } } Now, the urban legend.Ý } I had heard that a group (unnamed) was having a } vigorous discussion about the best way to strop } a steel knife so as to get the best edge for } cutting thin sections.Ý How many strokes, in } which direction, which side of the blade, etc.Ý } During the discussion, a bottle of Coke was } knocked off the table and broke into many } pieces.Ý } The story goes that one, or another, of these } people took up one of the shards, and suggested } that it would make a good knife edge. } Verification anyone? { } } Joel } } } Good morning, } Dear Joel, dear all } } I can't tell or verify the above mentioned } "Coke-shard-story" but - indeed - I can add } information as to the "knocked off } table"-part.... } } I had always heared (personal informations from } elder REICHERT-freaks and service people, from } lectures/lecturers in the 70ies and 80ies as } well as personally from H. Sitte himself) that } the group around H. SITTE at REICHERT (now } LEICA) in the late 1950ies/60ies was/were doing } such experiments with {glass plates} .... which } were thrown down to the floor... and one of them } (it was Sitte himself, as he told me) picked up } the shards evaluating the edges of "the most } promising one" for sectioning purposes (who of } that group had the "invention" to use glass as } a} crude { knife (element) I unfortunately don't } know. } } By the way, digging a little bit on this in } Google, I found one hint on a document in a } German data bank at } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver–ffentlicht } ("A simple ultramicrotome with thermal advance" published) } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } Note:Ý {Naturwissenschaften} is the former title } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } Another source:Ý http://www.freepatentsonline.com/3828641.html: } United States Patent US3828641: } } ==} Apparatus for adjusting the elevation of a } specimen in microtomes, particularly } ultramicrotomes } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } [as written in the patent document!]/Saar } Germany) } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } Filed:Ý Nov. 10,1972 } } Also see } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } (From the book Michael J. Dykstra, Laura E. } Reuss - 2003Ý (see page "History: in the text } displayed } the Reichert OMU-1 (before 1964, perhaps see } document above in {Naturwissenschaften} 1955) } from Sitte described as having thermal advance, } Sitte was never happy with, since 1964: OMU-2 } with thermal advance.... } } This just for your pleasure.... } (and I sure that searching for Sitte Hellmuth or } Sitte and microtome will find some results more } on that interesting and exciting "history" of } ultramicrotomy advances..... } } Best wishes and regards, } have a good and successful rest of the week and a beautiful weekend, } } Wolfgang MUSS (Ph.D.) } EM-Lab Gen.Hosp. SALK-LKH } SALZBURG } AUSTRIA } } } } } } } } } -----Urspr¸ngliche Nachricht----- } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } Gesendet: Donnerstag, 20. J”nner 2011 06:51 } } An: Muţ Wolfgang } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } ----------------------------------------------------------------------- } } ----- } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } America } } ToÝ Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } ----- } } } } It is certainly clear that there are many interesting stories --some } } urban legends, and some insights into the minds of those that founded } } the discipline of thin section electron microscopy. } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } microtome that I was told was developed by Sjostrand. } } This thing consisted of a disk-like plate, about 4-5 inches in } } diameter, with an eccentrically mounted arm that stuck out of the } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } The plate itself was mounted in a frame and lubricated with oil --the } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } on the wall by a long V belt (to minimize vibration), and the entire } } plate rotated, bringing the block past the knife on each rotation.Ý The } } sections that resulted tended to be arc shaped.Ý We got it working, but } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } remembers this type of machine -- assuming that my description is } } comprehensible. } } } } The other microtome that I used, and really admire, was the Huxley } } microtome, originally sold by Cambridge, and ultimately marketed by } } LKB.Ý Although it used a mechanical advance, all of the movements of } } the cutting arm were made by bending sheets of spring steel, so that } } there were no surfaces that moved against each other.Ý Moreover, the } } cutting stroke was controlled by an oil-filled damper, to minimize } } chatter. -- a remarkable and stable machine. } } } } Now, the urban legend. } } I had heard that a group (unnamed) was having a vigorous discussion } } about the best way to strop a steel knife so as to get the best edge } } for cutting thin sections.Ý How many strokes, in which direction, which } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } knocked off the table and broke into many pieces. } } The story goes that one, or another, of these people took up one of the } } shards, and suggested that it would make a good knife edge. } } Verification anyone? } } } } Joel } } } } -- } } Joel B. Sheffield, Ph.D. } } Biology Department, Temple University } } 1900 North 12th Street } } Philadelphia, PA 19122 } } jbs-at-temple.edu } } (215) 204 8839, fax (215) 204 0486 } } http://astro.temple.edu/~jbs
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 27 -- From oshel1pe-at-cmich.edu Thu Jan 20 09:53:21 2011 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFrLZ3030383 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:53:21 -0600 6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0KFrBHA005464 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:53:20 -0500 6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 6, 27 -- Thu, 20 Jan 2011 10:53:17 -0500 6, 27 -- Mime-Version: 1.0 6, 27 -- Message-Id: {a06240802c95e09c0c794-at-[141.209.160.249]} 6, 27 -- In-Reply-To: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} 6, 27 -- References: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} 6, 27 -- Date: Thu, 20 Jan 2011 10:53:12 -0500 6, 27 -- To: Microscopy-at-microscopy.com 6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 27 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" 6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 6, 27 -- X-OriginalArrivalTime: 20 Jan 2011 15:53:17.0197 (UTC) FILETIME=[273DA3D0:01CBB8BA] 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 27 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001:-0.5) 6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 27 -- X-CanItPRO-Stream: default 6, 27 -- X-Canit-Stats-ID: 02DVPRkyE - 9b55b8297af8 - 20110120 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KFrLZ3030383 ==============================End of - Headers==============================
I just remembered seeing a print ad from some science journal published the 40's or 50's (before my time!) for an early ultramicrotome. It was a high speed motor spinning some type of blade. The concept was that the block was advanced into this buzzsaw and you were supposed to catch the sections flying off. At the time I think the view was that ultrathins could only be cut at high speed. The real kicker was the ad mentioned the motor was also suitable for use in centrifuges. Crazy. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Thursday, January 20, 2011 9:54 AM To: Phillips, Thomas E.
I know at least 2 old microscopists who made glass knifes by breaking window glass - one of them here at CMU. He went to construction sites to get the broken windows.
I'm running down the reference and (I hope) an image, but one of the early tries at ultramicrotomy was sticking razor blades on a centrifuge rotor, then mount the sections on the tub, close the lid and turn on the centrifuge. The sections where then picked up from inside the tub, having been flung willy-nilly around the inside. (The image is used in the microtomy lecture to convince the students thin sectioning could be a lot worse than they think it is.)
Phil
} Von: Muţ Wolfgang } Gesendet: Donnerstag, 20. J"nner 2011 11:46 } An: 'joelsheffield-at-gmail.com' } Cc: 'microscopy-at-microscopy.com' } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } Joel wrote: } } Now, the urban legend.Ý } I had heard that a group (unnamed) was having a } vigorous discussion about the best way to strop } a steel knife so as to get the best edge for } cutting thin sections.Ý How many strokes, in } which direction, which side of the blade, etc.Ý } During the discussion, a bottle of Coke was } knocked off the table and broke into many } pieces.Ý } The story goes that one, or another, of these } people took up one of the shards, and suggested } that it would make a good knife edge. } Verification anyone? { } } Joel } } } Good morning, } Dear Joel, dear all } } I can't tell or verify the above mentioned } "Coke-shard-story" but - indeed - I can add } information as to the "knocked off } table"-part.... } } I had always heared (personal informations from } elder REICHERT-freaks and service people, from } lectures/lecturers in the 70ies and 80ies as } well as personally from H. Sitte himself) that } the group around H. SITTE at REICHERT (now } LEICA) in the late 1950ies/60ies was/were doing } such experiments with {glass plates} .... which } were thrown down to the floor... and one of them } (it was Sitte himself, as he told me) picked up } the shards evaluating the edges of "the most } promising one" for sectioning purposes (who of } that group had the "invention" to use glass as } a} crude { knife (element) I unfortunately don't } know. } } By the way, digging a little bit on this in } Google, I found one hint on a document in a } German data bank at } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver-ffentlicht } ("A simple ultramicrotome with thermal advance" published) } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } Note:Ý {Naturwissenschaften} is the former title } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } Another source:Ý http://www.freepatentsonline.com/3828641.html: } United States Patent US3828641: } } ==} Apparatus for adjusting the elevation of a } specimen in microtomes, particularly } ultramicrotomes } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } [as written in the patent document!]/Saar } Germany) } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } Filed:Ý Nov. 10,1972 } } Also see } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } (From the book Michael J. Dykstra, Laura E. } Reuss - 2003Ý (see page "History: in the text } displayed } the Reichert OMU-1 (before 1964, perhaps see } document above in {Naturwissenschaften} 1955) } from Sitte described as having thermal advance, } Sitte was never happy with, since 1964: OMU-2 } with thermal advance.... } } This just for your pleasure.... } (and I sure that searching for Sitte Hellmuth or } Sitte and microtome will find some results more } on that interesting and exciting "history" of } ultramicrotomy advances..... } } Best wishes and regards, } have a good and successful rest of the week and a beautiful weekend, } } Wolfgang MUSS (Ph.D.) } EM-Lab Gen.Hosp. SALK-LKH } SALZBURG } AUSTRIA } } } } } } } } } -----Urspr¸ngliche Nachricht----- } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } Gesendet: Donnerstag, 20. J"nner 2011 06:51 } } An: Muţ Wolfgang } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } ----------------------------------------------------------------------- } } ----- } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } America } } ToÝ Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } ----- } } } } It is certainly clear that there are many interesting stories --some } } urban legends, and some insights into the minds of those that founded } } the discipline of thin section electron microscopy. } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } microtome that I was told was developed by Sjostrand. } } This thing consisted of a disk-like plate, about 4-5 inches in } } diameter, with an eccentrically mounted arm that stuck out of the } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } The plate itself was mounted in a frame and lubricated with oil --the } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } on the wall by a long V belt (to minimize vibration), and the entire } } plate rotated, bringing the block past the knife on each rotation.Ý The } } sections that resulted tended to be arc shaped.Ý We got it working, but } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } remembers this type of machine -- assuming that my description is } } comprehensible. } } } } The other microtome that I used, and really admire, was the Huxley } } microtome, originally sold by Cambridge, and ultimately marketed by } } LKB.Ý Although it used a mechanical advance, all of the movements of } } the cutting arm were made by bending sheets of spring steel, so that } } there were no surfaces that moved against each other.Ý Moreover, the } } cutting stroke was controlled by an oil-filled damper, to minimize } } chatter. -- a remarkable and stable machine. } } } } Now, the urban legend. } } I had heard that a group (unnamed) was having a vigorous discussion } } about the best way to strop a steel knife so as to get the best edge } } for cutting thin sections.Ý How many strokes, in which direction, which } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } knocked off the table and broke into many pieces. } } The story goes that one, or another, of these people took up one of the } } shards, and suggested that it would make a good knife edge. } } Verification anyone? } } } } Joel } } } } -- } } Joel B. Sheffield, Ph.D. } } Biology Department, Temple University } } 1900 North 12th Street } } Philadelphia, PA 19122 } } jbs-at-temple.edu } } (215) 204 8839, fax (215) 204 0486 } } http://astro.temple.edu/~jbs
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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==============================Original Headers============================== 16, 35 -- From PhillipsT-at-missouri.edu Thu Jan 20 10:20:18 2011 16, 35 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KGKG9E014299 16, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:20:16 -0600 16, 35 -- X-IronPort-Anti-Spam-Filtered: true 16, 35 -- X-IronPort-Anti-Spam-Result: AvsEAH7vN03RauUp/2dsb2JhbACkUXO2AYheAoJuIoI+BIRviVmCcw 16, 35 -- Received: from um-nsmtpout1.um.umsystem.edu ([209.106.229.41]) 16, 35 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Jan 2011 10:20:14 -0600 16, 35 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 16, 35 -- Thu, 20 Jan 2011 10:19:55 -0600 16, 35 -- Received: from UM-MSXHUB05.um.umsystem.edu (131.151.121.205) by 16, 35 -- UM-THUB01.um.umsystem.edu (209.106.230.181) with Microsoft SMTP Server (TLS) 16, 35 -- id 8.2.254.0; Thu, 20 Jan 2011 10:19:55 -0600 16, 35 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 16, 35 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Thu, 20 Jan 2011 16, 35 -- 10:19:54 -0600 16, 35 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 16, 35 -- Date: Thu, 20 Jan 2011 10:19:53 -0600 16, 35 -- Subject: [Microscopy] Re: Microtome History 16, 35 -- Thread-Topic: [Microscopy] Re: Microtome History 16, 35 -- Thread-Index: Acu4ujnK/W+eYZMgQe2m/DmfMJG5qAAAyCyg 16, 35 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF1C4596EF46-at-UM-EMAIL05.um.umsystem.edu} 16, 35 -- References: {201101201553.p0KFrlKj031360-at-ns.microscopy.com} 16, 35 -- In-Reply-To: {201101201553.p0KFrlKj031360-at-ns.microscopy.com} 16, 35 -- Accept-Language: en-US 16, 35 -- Content-Language: en-US 16, 35 -- X-MS-Has-Attach: 16, 35 -- X-MS-TNEF-Correlator: 16, 35 -- acceptlanguage: en-US 16, 35 -- Content-Type: text/plain; charset="iso-8859-1" 16, 35 -- MIME-Version: 1.0 16, 35 -- X-OriginalArrivalTime: 20 Jan 2011 16:19:55.0726 (UTC) FILETIME=[E009CEE0:01CBB8BD] 16, 35 -- Content-Transfer-Encoding: 8bit 16, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KGKG9E014299 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mmgraham06-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mmgraham06-at-gmail.com Name: Morven Graham
Organization: Yale School of Medicine
Title-Subject: [Filtered] Re:Research Assistant Position
Message: Hi Listeners
There is a current opening for a EM technician at Yale University Medical School.
I had a Topcon 002B at Intel for many years for which use of a hammer was required. The ion pump would periodically grow whiskers that resulted in markedly increased ion current. The best way to get rid of them was to bang on the pump with a hammer. I don't mean a rubber mallet either. To be fully effective, this required a big iron hammer and hearing protection, as well as thick skin to withstand the curious stares and complaints about the noise and questions about one's sanity from fellow workers.
John Mardinly
Western Digital
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: Thursday, January 20, 2011 7:44 AM To: John Mardinly
Forgot how much fun these discussions of the "old days" can be. At Washington University there was an old Seimens microscope. At the time I was training as a tech, and using the microscope was not an option. However, the man who trained me, Charles Kuhn, told me that one of the microscopes he had used, and I believe he was referring to the old Seimens at WU, had to be aligned using a rubber mallet. Never did it personally, but was regaled with stories by one who did.
paul -- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 3, 21 -- From paul_hazelton-at-umanitoba.ca Thu Jan 20 09:36:45 2011 3, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFaitU013986 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:36:44 -0600 3, 21 -- Received: from [140.193.25.116] (basic116.medmb.umanitoba.ca [140.193.25.116]) 3, 21 -- (user=hazeltn mech=CRAM-MD5 bits=0) 3, 21 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0KFahNr022457 3, 21 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO); 3, 21 -- Thu, 20 Jan 2011 09:36:43 -0600 (CST) 3, 21 -- Message-ID: {4D38568A.2050009-at-umanitoba.ca} 3, 21 -- Date: Thu, 20 Jan 2011 09:36:42 -0600 3, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 3, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 3, 21 -- MIME-Version: 1.0 3, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 21 -- Subject: Re: [Microscopy] RE: Early microtomy--urban legend? 3, 21 -- References: {201101201456.p0KEu0Bl002900-at-ns.microscopy.com} 3, 21 -- In-Reply-To: {201101201456.p0KEu0Bl002900-at-ns.microscopy.com} 3, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 21 -- Content-Transfer-Encoding: 7bit 3, 21 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
==============================Original Headers============================== 12, 30 -- From prvs=994c3f914=John.Mardinly-at-wdc.com Thu Jan 20 11:10:24 2011 12, 30 -- Received: from wdscspam2.wdc.com (wdscspam2.wdc.com [129.253.55.43]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KHAMhs015780 12, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:10:23 -0600 12, 30 -- X-IronPort-AV: E=Sophos;i="4.60,352,1291622400"; 12, 30 -- d="scan'208";a="172510791" 12, 30 -- Received: from unknown (HELO wdscexfe02.sc.wdc.com) ([172.25.19.2]) 12, 30 -- by wdscspam2.wdc.com with ESMTP; 20 Jan 2011 09:10:15 -0800 12, 30 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe02.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.4675); 12, 30 -- Thu, 20 Jan 2011 09:10:19 -0800 12, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 30 -- Content-class: urn:content-classes:message 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Type: text/plain; 12, 30 -- charset="us-ascii" 12, 30 -- Subject: Hammer Required 12, 30 -- Date: Thu, 20 Jan 2011 09:10:19 -0800 12, 30 -- Message-ID: {34061960C62E274C8AF1DCAA65655558097AA6D7-at-wdksjexbe01.msj.wdc.com} 12, 30 -- In-Reply-To: {201101201544.p0KFiE15027036-at-ns.microscopy.com} 12, 30 -- X-MS-Has-Attach: 12, 30 -- X-MS-TNEF-Correlator: 12, 30 -- Thread-Topic: Hammer Required 12, 30 -- Thread-Index: Acu4uOR02gkLoEhtTJeOGW8TB0f+tgAC1YWg 12, 30 -- References: {201101201544.p0KFiE15027036-at-ns.microscopy.com} 12, 30 -- From: "John Mardinly" {John.Mardinly-at-wdc.com} 12, 30 -- To: {paul_hazelton-at-umanitoba.ca} 12, 30 -- Cc: {Microscopy-at-microscopy.com} 12, 30 -- X-OriginalArrivalTime: 20 Jan 2011 17:10:19.0902 (UTC) FILETIME=[EA9671E0:01CBB8C4] 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KHAMhs015780 ==============================End of - Headers==============================
Indeed. We are in a building that was often broken into. Years ago, I made it my business to collect the old door panes --they were a tinted glass, about 1/4" thick, and had just the right temper to make excellent knives.
Joel
On Thu, Jan 20, 2011 at 10:59 AM, {oshel1pe-at-cmich.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I know at least 2 old microscopists who made } glass knifes by breaking window glass - one of } them here at CMU. He went to construction sites } to get the broken windows. } } I'm running down the reference and (I hope) an } image, but one of the early tries at } ultramicrotomy was sticking razor blades on a } centrifuge rotor, then mount the sections on the } tub, close the lid and turn on the centrifuge. } The sections where then picked up from inside the } tub, having been flung willy-nilly around the } inside. } (The image is used in the microtomy lecture to } convince the students thin sectioning could be a } lot worse than they think it is.) } } Phil } } } Von: Muţ Wolfgang } } Gesendet: Donnerstag, 20. J”nner 2011 11:46 } } An: 'joelsheffield-at-gmail.com' } } Cc: 'microscopy-at-microscopy.com' } } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } Joel wrote: } } } Now, the urban legend.Ý } } I had heard that a group (unnamed) was having a } } vigorous discussion about the best way to strop } } a steel knife so as to get the best edge for } } cutting thin sections.Ý How many strokes, in } } which direction, which side of the blade, etc.Ý } } During the discussion, a bottle of Coke was } } knocked off the table and broke into many } } pieces.Ý } } The story goes that one, or another, of these } } people took up one of the shards, and suggested } } that it would make a good knife edge. } } Verification anyone? { } } } } Joel } } } } } } Good morning, } } Dear Joel, dear all } } } } I can't tell or verify the above mentioned } } "Coke-shard-story" but - indeed - I can add } } information as to the "knocked off } } table"-part.... } } } } I had always heared (personal informations from } } elder REICHERT-freaks and service people, from } } lectures/lecturers in the 70ies and 80ies as } } well as personally from H. Sitte himself) that } } the group around H. SITTE at REICHERT (now } } LEICA) in the late 1950ies/60ies was/were doing } } such experiments with {glass plates} .... which } } were thrown down to the floor... and one of them } } (it was Sitte himself, as he told me) picked up } } the shards evaluating the edges of "the most } } promising one" for sectioning purposes (who of } } that group had the "invention" to use glass as } } a} crude { knife (element) I unfortunately don't } } know. } } } } By the way, digging a little bit on this in } } Google, I found one hint on a document in a } } German data bank at } } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver–ffentlicht } } ("A simple ultramicrotome with thermal advance" published) } } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } } } Note:Ý {Naturwissenschaften} is the former title } } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } } Another source:Ý http://www.freepatentsonline.com/3828641.html: } } United States Patent US3828641: } } } } ==} Apparatus for adjusting the elevation of a } } specimen in microtomes, particularly } } ultramicrotomes } } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } } [as written in the patent document!]/Saar } } Germany) } } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } } Filed:Ý Nov. 10,1972 } } } } Also see } } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } } (From the book Michael J. Dykstra, Laura E. } } Reuss - 2003Ý (see page "History: in the text } } displayed } } the Reichert OMU-1 (before 1964, perhaps see } } document above in {Naturwissenschaften} 1955) } } from Sitte described as having thermal advance, } } Sitte was never happy with, since 1964: OMU-2 } } with thermal advance.... } } } } This just for your pleasure.... } } (and I sure that searching for Sitte Hellmuth or } } Sitte and microtome will find some results more } } on that interesting and exciting "history" of } } ultramicrotomy advances..... } } } } Best wishes and regards, } } have a good and successful rest of the week and a beautiful weekend, } } } } Wolfgang MUSS (Ph.D.) } } EM-Lab Gen.Hosp. SALK-LKH } } SALZBURG } } AUSTRIA } } } } } } } } } } } } } } } } } -----Urspr¸ngliche Nachricht----- } } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } } Gesendet: Donnerstag, 20. J”nner 2011 06:51 } } } An: Muţ Wolfgang } } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } } America } } } ToÝ Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } } } ----- } } } } } } It is certainly clear that there are many interesting stories --some } } } urban legends, and some insights into the minds of those that founded } } } the discipline of thin section electron microscopy. } } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } } microtome that I was told was developed by Sjostrand. } } } This thing consisted of a disk-like plate, about 4-5 inches in } } } diameter, with an eccentrically mounted arm that stuck out of the } } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } } The plate itself was mounted in a frame and lubricated with oil --the } } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } } on the wall by a long V belt (to minimize vibration), and the entire } } } plate rotated, bringing the block past the knife on each rotation.Ý The } } } sections that resulted tended to be arc shaped.Ý We got it working, but } } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } } remembers this type of machine -- assuming that my description is } } } comprehensible. } } } } } } The other microtome that I used, and really admire, was the Huxley } } } microtome, originally sold by Cambridge, and ultimately marketed by } } } LKB.Ý Although it used a mechanical advance, all of the movements of } } } the cutting arm were made by bending sheets of spring steel, so that } } } there were no surfaces that moved against each other.Ý Moreover, the } } } cutting stroke was controlled by an oil-filled damper, to minimize } } } chatter. -- a remarkable and stable machine. } } } } } } Now, the urban legend. } } } I had heard that a group (unnamed) was having a vigorous discussion } } } about the best way to strop a steel knife so as to get the best edge } } } for cutting thin sections.Ý How many strokes, in which direction, which } } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } } knocked off the table and broke into many pieces. } } } The story goes that one, or another, of these people took up one of the } } } shards, and suggested that it would make a good knife edge. } } } Verification anyone? } } } } } } Joel } } } } } } -- } } } Joel B. Sheffield, Ph.D. } } } Biology Department, Temple University } } } 1900 North 12th Street } } } Philadelphia, PA 19122 } } } jbs-at-temple.edu } } } (215) 204 8839, fax (215) 204 0486 } } } http://astro.temple.edu/~jbs } } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } (989) 774-3576 } } } ==============================Original Headers============================== } 6, 27 -- From oshel1pe-at-cmich.edu Thu Jan 20 09:53:21 2011 } 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFrLZ3030383 } 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:53:21 -0600 } 6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0KFrBHA005464 } 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:53:20 -0500 } 6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); } 6, 27 -- Thu, 20 Jan 2011 10:53:17 -0500 } 6, 27 -- Mime-Version: 1.0 } 6, 27 -- Message-Id: {a06240802c95e09c0c794-at-[141.209.160.249]} } 6, 27 -- In-Reply-To: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} } 6, 27 -- References: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} } 6, 27 -- Date: Thu, 20 Jan 2011 10:53:12 -0500 } 6, 27 -- To: Microscopy-at-microscopy.com } 6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } 6, 27 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" } 6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 6, 27 -- X-OriginalArrivalTime: 20 Jan 2011 15:53:17.0197 (UTC) FILETIME=[273DA3D0:01CBB8BA] } 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) } 6, 27 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001:-0.5) } 6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 } 6, 27 -- X-CanItPRO-Stream: default } 6, 27 -- X-Canit-Stats-ID: 02DVPRkyE - 9b55b8297af8 - 20110120 } 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 } 6, 27 -- Content-Transfer-Encoding: 8bit } 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KFrLZ3030383 } ==============================End of - Headers============================== }
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 7, 35 -- From joelsheffield-at-gmail.com Thu Jan 20 11:31:19 2011 7, 35 -- Received: from mail-ww0-f41.google.com (mail-ww0-f41.google.com [74.125.82.41]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KHVHTs032381 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:31:18 -0600 7, 35 -- Received: by wwi18 with SMTP id 18so1718115wwi.0 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:31:16 -0800 (PST) 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=domainkey-signature:mime-version:in-reply-to:references:date 7, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 7, 35 -- bh=0t+PYP/uH2Mdg2F0J7RJ8voEOJFKxX7bczfl+eGG/Co=; 7, 35 -- b=cbjmwQQucf57+U/vclDM54yIhxJrPqlb7rnwS8YgK1VQ9rIgRheH1j2tzOjk0fpzZ/ 7, 35 -- HQVgzLPBASgodYlvxv7vYXdZQ8ElKg8dSosDGJoAyxiBSJgz/4p6tgqpPWB+33yz6bhj 7, 35 -- /VjCG95PXDhJ2NNRliP6JrVWIGXY83BIAqwaA= 7, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 7, 35 -- :content-type:content-transfer-encoding; 7, 35 -- b=ANU0y+3BwOi1bDDls16xlG64Hpm/SyR/j8iJyVxzEjzc65G9X1p76jnbH2/yyYbcHg 7, 35 -- S8J7OUwpvJyyYJiQM+0WgkzQF1vAyRiywAavPcKpfu8fwYBJhCLhcBQjPb27j2TQsVP+ 7, 35 -- cOw/cILd8E/daSyBM6ZFGyjKpC4QaiHr2m5vk= 7, 35 -- MIME-Version: 1.0 7, 35 -- Received: by 10.227.157.19 with SMTP id z19mr2626557wbw.189.1295544676290; 7, 35 -- Thu, 20 Jan 2011 09:31:16 -0800 (PST) 7, 35 -- Received: by 10.227.143.213 with HTTP; Thu, 20 Jan 2011 09:31:14 -0800 (PST) 7, 35 -- In-Reply-To: {201101201559.p0KFxBRa009241-at-ns.microscopy.com} 7, 35 -- References: {201101201559.p0KFxBRa009241-at-ns.microscopy.com} 7, 35 -- Date: Thu, 20 Jan 2011 12:31:14 -0500 7, 35 -- Message-ID: {AANLkTimynhsc+65Ai6DSg8gWQ=P6RaJZjn0ZWY7TB-og-at-mail.gmail.com} 7, 35 -- Subject: Re: [Microscopy] Re: Microtome History - was "Ernst Ruska" 7, 35 -- From: Joel Sheffield {joelsheffield-at-gmail.com} 7, 35 -- To: oshel1pe-at-cmich.edu, microscopy-at-microscopy.com 7, 35 -- Content-Type: text/plain; charset=windows-1252 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KHVHTs032381 ==============================End of - Headers==============================
I can confirm the rubber mallet story for column alignment. I worked at Mellon Institute in Pittsburgh in the '70's. So did my father, Martin Haller, who taught me electron Microscopy. He worked there from the '60's to the '70's. We had several RCA electron microscopes that were purchased many years before I was trained in microscopy. My father used to align the lenses in the column of these microscopes with a rubber mallet. I took a couple of photos on these microscopes back in 1974. They shot glass plates with 3 exposures. After the 3 shots, you had to break vacuum, take out your plate, replace it and repump the scope to be able to take any more photos.
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: PhillipsT-at-missouri.edu [PhillipsT-at-missouri.edu] Sent: Thursday, January 20, 2011 10:06 AM To: Haller, Edward
I have heard all the microtome and glass knives stories also but have no evidence to their veracity. But I once was talking to Sanford Palay - an early pioneer in EM - and he told me how they used a rubber hammer to tap the outside of the TEM column to try and nudge the aperture into place. I can't imagine how tedious that must have been. Of course, the reward the pioneers all got from primitive microtomy and TEMs was that they got to discover amazing new things of a bigger magnitude than most of the incremental advances we all get to contribute to these days. But I think I would rather have done science then rather than now when high cost forces specialization and a greater proportion of time is devoted to finding funding rather than organelles. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu] Sent: Thursday, January 20, 2011 8:20 AM To: Phillips, Thomas E.
Fellow microscopists:
I was taught decades ago that early ultrathin sections were made in the following manner:
A sheet of plate glass was dropped on the floor. The shard with the best edge was selected and attached to the blade of a small window fan. The resin-embedded specimen was attached to the tip of a soldering iron. once everything was clamped and aligned, the fan was turned on, the soldering iron was plugged in, and the section were collected in a large pan of water held beneath the fan. (As the soldering iron heated, the thermal expansion advanced the specimen into the glass blade.) Sections of optimal thickness were selected from those floating in the pan on the basis of the color refracted.
I love the story. Does anyone know whether this even approaches a true story?
Thanks,
Don
P.S. Anyone still alive who has first-hand knowledge of this story would be pretty old by now, but I thought that I would give this a shot.
--
Donald L. Lovett e-mail: lovett-at-tcnj.edu Professor and Chairperson phone: 609-771-2876 Department of Biology fax: 609-637-5118 The College of New Jersey P.O. Box 7718 Ewing, NJ 08628-0718
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==============================Original Headers============================== 19, 32 -- From PhillipsT-at-missouri.edu Thu Jan 20 08:54:00 2011 19, 32 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KErxrG030207 19, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:54:00 -0600 19, 32 -- X-IronPort-Anti-Spam-Filtered: true 19, 32 -- X-IronPort-Anti-Spam-Result: AvsEAN7aN03RauUo/2dsb2JhbACkUXO1cYhEAoMQgj4EhG+BN4Qsg3Y 19, 32 -- Received: from um-tsmtpout1.um.umsystem.edu ([209.106.229.40]) 19, 32 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Jan 2011 08:53:59 -0600 19, 32 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 19, 32 -- Thu, 20 Jan 2011 08:53:59 -0600 19, 32 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 19, 32 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Thu, 20 Jan 2011 19, 32 -- 08:53:58 -0600 19, 32 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 19, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 32 -- Date: Thu, 20 Jan 2011 08:53:54 -0600 19, 32 -- Subject: RE: [Microscopy] Early microtomy--urban legend? 19, 32 -- Thread-Topic: [Microscopy] Early microtomy--urban legend? 19, 32 -- Thread-Index: Acu4rTCfGg2oH1rLRqikoaKr1g1gsgAA+QUQ 19, 32 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF1C4596EF1A-at-UM-EMAIL05.um.umsystem.edu} 19, 32 -- References: {201101201420.p0KEKJfH031321-at-ns.microscopy.com} 19, 32 -- In-Reply-To: {201101201420.p0KEKJfH031321-at-ns.microscopy.com} 19, 32 -- Accept-Language: en-US 19, 32 -- Content-Language: en-US 19, 32 -- X-MS-Has-Attach: 19, 32 -- X-MS-TNEF-Correlator: 19, 32 -- acceptlanguage: en-US 19, 32 -- Content-Type: text/plain; charset="us-ascii" 19, 32 -- MIME-Version: 1.0 19, 32 -- X-OriginalArrivalTime: 20 Jan 2011 14:53:59.0017 (UTC) FILETIME=[DE667590:01CBB8B1] 19, 32 -- Content-Transfer-Encoding: 8bit 19, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KErxrG030207 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 37 -- From ehaller-at-health.usf.edu Thu Jan 20 11:43:12 2011 26, 37 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 26, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KHhCcS016439 26, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:43:12 -0600 26, 37 -- X-ASG-Debug-ID: 1295545391-2cee01ca0000-4CH8be 26, 37 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-bin/mark.cgi 26, 37 -- Received: from mailhub1.hscnet.hsc.usf.edu (localhost [127.0.0.1]) 26, 37 -- by hscantispam.health.usf.edu (Spam & Virus Firewall) with ESMTP 26, 37 -- id 192D7790BEDE; Thu, 20 Jan 2011 12:43:11 -0500 (EST) 26, 37 -- Received: from mailhub1.hscnet.hsc.usf.edu ([10.119.4.12]) by hscantispam.health.usf.edu with ESMTP id vWDQSRI9gyDKBfzE; Thu, 20 Jan 2011 12:43:11 -0500 (EST) 26, 37 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 26, 37 -- Received: from MAILSERVER3.hscnet.hsc.usf.edu ([10.119.4.19]) by 26, 37 -- mailhub1.hscnet.hsc.usf.edu ([10.119.4.12]) with mapi; Thu, 20 Jan 2011 26, 37 -- 12:43:11 -0500 26, 37 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 26, 37 -- To: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} , 26, 37 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 26, 37 -- Date: Thu, 20 Jan 2011 12:43:11 -0500 26, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: Early microtomy--urban legend? 26, 37 -- Subject: RE: [Microscopy] RE: Early microtomy--urban legend? 26, 37 -- Thread-Topic: [Microscopy] RE: Early microtomy--urban legend? 26, 37 -- Thread-Index: Acu4s4/fp3dncIDRQdqcJkxODIBNBwAFS1Bc 26, 37 -- Message-ID: {5B34016F48340D428A2644D479F587FC359C34E3-at-MAILSERVER3.hscnet.hsc.usf.edu} 26, 37 -- References: {201101201506.p0KF64df001518-at-ns.microscopy.com} 26, 37 -- In-Reply-To: {201101201506.p0KF64df001518-at-ns.microscopy.com} 26, 37 -- Accept-Language: en-US 26, 37 -- Content-Language: en-US 26, 37 -- X-MS-Has-Attach: 26, 37 -- X-MS-TNEF-Correlator: 26, 37 -- acceptlanguage: en-US 26, 37 -- Content-Type: text/plain; charset="us-ascii" 26, 37 -- MIME-Version: 1.0 26, 37 -- X-Barracuda-Connect: UNKNOWN[10.119.4.12] 26, 37 -- X-Barracuda-Start-Time: 1295545392 26, 37 -- X-Barracuda-Virus-Scanned: by USF Health AntiSpam System at health.usf.edu 26, 37 -- Content-Transfer-Encoding: 8bit 26, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KHhCcS016439 ==============================End of - Headers==============================
Dear All, I had the same problem visualizing bee wax structures and not owning an ESEM ;-)
Solution: I put the structure on a large specimen mount, putting 2 bands of conductive adhesive tape left and right on top and put it in my normal Pt-sputtercoater. Concerning the heat during sputtering, I took off the sputter-head and added a second glass vessel (I took it from my carbon-coater) on top of the first. Did ca. 10-15 sputtering-runs with minimal current (10mA) and finally put it in the scope at 5-6kV.
See images at http://www.electronmicroscopy.info/wax
It`s a bit of a crude solution but it works. Concerning the thickness of your wax layers you may also try the same thing with an high-vac chromecoater...
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Dear Zhou, If possible, I would put in a very small SA aperture and try to use a parallel beam to get Bragg spots. These precipitates are very small, so you may need to take long exposures, which means that the specimen and aperture positions must be very stable. In order to see diffraction from the small particles, the diffraction from the much larger surrounding area must be minimized (or subtracted out). Yours, Bill
X-from: z.zhou-at-lboro.ac.uk To: William.F.Tivol-at-aero.org
Organization: Dept of Materials, Loughborough University, UK
Title-Subject: [Filtered] TEM diffraction 3nm precipitates
Message: Greetings listers,
Iím studying crystal structure of some carbides/nitrides precipitates in steel. The precipitates are 2-5nm size in a C replica TEM specimen, V-, Cr-, and Nb-rich by STEM/EDX.
How can I get electron diffraction or crystal structure information of these fine precipitates?
I tried on a TecnaiF20 TEM using nanoprobe spot 7, focused on a small particle, then diffraction, most of the time I got amorphous rings of C support, no obvious diffraction discs. STEM mode did indicate some C contamination on the session. Assuming minimal C contamination, is it possible that this method can give me some sort of convergence beam electron diffraction pattern, which may help me determine the crystal structure of the precipitates?
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Dear Frank, The key is that you're "looking for slight changes in element concentrations at grain boundaries and precipitates." In order best to see these changes, the pixel size should be smaller than the features--half the feature size guarantees that at least one pixel will be entirely within the feature. If the pixel size is too large than you will measure the average element concentrations over both the feature and the surrounding area, which will make the apparent changes smaller than they really are. I would suggest taking an image to locate the features, then place the beam on each feature in turn to do the analysis. Also take a spectrum of the area next to each feature. This should give you the best chance of seeing differences and minimize the amount of data. Yours, Bill
X-from: Frank_Karl-at-lincolnelectric.com To: William.F.Tivol-at-aero.org
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Iâ??ve been thinking about EDS line scans and Iâ??m attempting to maximize the data I collect.
I run at 20 KV on iron samples so my electron interaction range is between 1.5 to 2.3um (Berthe or K-O range calculations). According to data provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um at 700x. So right away I see if each image pixel represents a data point, Iâ??m sampling about 2 pixel volumes per data point. Looking for a sudden and sharp change in element concentration seem difficult, but wait thereâ??s more. Let us introduce another plot complication.
My customers donâ??t want to scan the entire image, tooo much dataâ?¦
So I scan a smaller section of image described above and instruct the computer to take 512 data points along a line that might only be 400 (visual estimate from screen) image pixels long.
Soâ?¦ Should I reduce the image resolution so that each image pixel will be one data point and scan the entire image? Would it be better to lower the magnification so the image pixels are larger (say 1.2um ). I could go to 100x and 512 horizontal image pixel so each image pixel is 2.3um?
Iâ??m looking for slight changes in element concentration at grain boundaries and precipitates and of course I want to produce the most meaningful data possible. Iâ??m running at conditions that suggest my electron interactive volume is larger than my spatial resolution as to produce â??continuousâ?ť data.
Or am I just over thinking the process?
Any suggestion or comments would be welcome
Frank Lincoln Electric Company -- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 11, 17 -- From frank_karl-at-lincolnelectric.com Wed Jan 19 07:37:23 2011 11, 17 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JDbNoO031503 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 07:37:23 -0600 11, 17 -- Subject: EDS line scans and time on my hands 11, 17 -- To: Microscopy-at-microscopy.com 11, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 11, 17 -- Message-ID: {OF4A811DE5.D47D1408-ON8525781D.004A569D-8525781D.004AD372-at-lincolnelectric.com} 11, 17 -- From: Frank_Karl-at-lincolnelectric.com 11, 17 -- Date: Wed, 19 Jan 2011 08:38:03 -0500 11, 17 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 11, 17 -- 01/19/2011 08:37:18 AM 11, 17 -- MIME-Version: 1.0 11, 17 -- Content-Type: text/plain; 11, 17 -- charset="UTF-8" 11, 17 -- Content-Transfer-Encoding: 8bit 11, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p0JDbNoO031503 ==============================End of - Headers==============================
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This sounds more like a food processor than a microtome!
John Mardinly
Western Digital
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Thursday, January 20, 2011 8:30 AM To: John Mardinly
I just remembered seeing a print ad from some science journal published the 40's or 50's (before my time!) for an early ultramicrotome. It was a high speed motor spinning some type of blade. The concept was that the block was advanced into this buzzsaw and you were supposed to catch the sections flying off. At the time I think the view was that ultrathins could only be cut at high speed. The real kicker was the ad mentioned the motor was also suitable for use in centrifuges. Crazy. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Thursday, January 20, 2011 9:54 AM To: Phillips, Thomas E.
I know at least 2 old microscopists who made glass knifes by breaking window glass - one of them here at CMU. He went to construction sites to get the broken windows.
I'm running down the reference and (I hope) an image, but one of the early tries at ultramicrotomy was sticking razor blades on a centrifuge rotor, then mount the sections on the tub, close the lid and turn on the centrifuge. The sections where then picked up from inside the tub, having been flung willy-nilly around the inside. (The image is used in the microtomy lecture to convince the students thin sectioning could be a lot worse than they think it is.)
Phil
} Von: Mu Wolfgang } Gesendet: Donnerstag, 20. J"nner 2011 11:46 } An: 'joelsheffield-at-gmail.com' } Cc: 'microscopy-at-microscopy.com' } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } Joel wrote: } } Now, the urban legend. } I had heard that a group (unnamed) was having a } vigorous discussion about the best way to strop } a steel knife so as to get the best edge for } cutting thin sections. How many strokes, in } which direction, which side of the blade, etc. } During the discussion, a bottle of Coke was } knocked off the table and broke into many } pieces. } The story goes that one, or another, of these } people took up one of the shards, and suggested } that it would make a good knife edge. } Verification anyone? { } } Joel } } } Good morning, } Dear Joel, dear all } } I can't tell or verify the above mentioned } "Coke-shard-story" but - indeed - I can add } information as to the "knocked off } table"-part.... } } I had always heared (personal informations from } elder REICHERT-freaks and service people, from } lectures/lecturers in the 70ies and 80ies as } well as personally from H. Sitte himself) that } the group around H. SITTE at REICHERT (now } LEICA) in the late 1950ies/60ies was/were doing } such experiments with {glass plates} .... which } were thrown down to the floor... and one of them } (it was Sitte himself, as he told me) picked up } the shards evaluating the edges of "the most } promising one" for sectioning purposes (who of } that group had the "invention" to use glass as } a} crude { knife (element) I unfortunately don't } know. } } By the way, digging a little bit on this in } Google, I found one hint on a document in a } German data bank at } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H. } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver-ffentlicht } ("A simple ultramicrotome with thermal advance" published) } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } Note: {Naturwissenschaften} is the former title } of Biomedical and Life Sciences (SPRINGER) } Another source: http://www.freepatentsonline.com/3828641.html: } United States Patent US3828641: } } ==} Apparatus for adjusting the elevation of a } specimen in microtomes, particularly } ultramicrotomes } Inventor: Hellmuth Sitte, HOMBURG (not Humburg } [as written in the patent document!]/Saar } Germany) } Assignee: C.Reichert Optische Werke AG, Vienna, Austria) } Filed: Nov. 10,1972 } } Also see } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+an d+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl =de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CC EQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } (From the book Michael J. Dykstra, Laura E. } Reuss - 2003 (see page "History: in the text } displayed } the Reichert OMU-1 (before 1964, perhaps see } document above in {Naturwissenschaften} 1955) } from Sitte described as having thermal advance, } Sitte was never happy with, since 1964: OMU-2 } with thermal advance.... } } This just for your pleasure.... } (and I sure that searching for Sitte Hellmuth or } Sitte and microtome will find some results more } on that interesting and exciting "history" of } ultramicrotomy advances..... } } Best wishes and regards, } have a good and successful rest of the week and a beautiful weekend, } } Wolfgang MUSS (Ph.D.) } EM-Lab Gen.Hosp. SALK-LKH } SALZBURG } AUSTRIA } } } } } } } } } -----Ursprngliche Nachricht----- } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } Gesendet: Donnerstag, 20. J"nner 2011 06:51 } } An: Mu Wolfgang } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } ----------------------------------------------------------------------- } } ----- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } ----- } } } } It is certainly clear that there are many interesting stories --some } } urban legends, and some insights into the minds of those that founded } } the discipline of thin section electron microscopy. } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } microtome that I was told was developed by Sjostrand. } } This thing consisted of a disk-like plate, about 4-5 inches in } } diameter, with an eccentrically mounted arm that stuck out of the } } plate. The arm had a chuck for a tissue sample, and a heating coil. } } The plate itself was mounted in a frame and lubricated with oil --the } } plate acted like a large oil bearing. The plate was linked to a motor } } on the wall by a long V belt (to minimize vibration), and the entire } } plate rotated, bringing the block past the knife on each rotation. The } } sections that resulted tended to be arc shaped. We got it working, but } } it was a terrifying thing to see. I wonder if anyone in this group } } remembers this type of machine -- assuming that my description is } } comprehensible. } } } } The other microtome that I used, and really admire, was the Huxley } } microtome, originally sold by Cambridge, and ultimately marketed by } } LKB. Although it used a mechanical advance, all of the movements of } } the cutting arm were made by bending sheets of spring steel, so that } } there were no surfaces that moved against each other. Moreover, the } } cutting stroke was controlled by an oil-filled damper, to minimize } } chatter. -- a remarkable and stable machine. } } } } Now, the urban legend. } } I had heard that a group (unnamed) was having a vigorous discussion } } about the best way to strop a steel knife so as to get the best edge } } for cutting thin sections. How many strokes, in which direction, which } } side of the blade, etc. During the discussion, a bottle of Coke was } } knocked off the table and broke into many pieces. } } The story goes that one, or another, of these people took up one of the } } shards, and suggested that it would make a good knife edge. } } Verification anyone? } } } } Joel } } } } -- } } Joel B. Sheffield, Ph.D. } } Biology Department, Temple University } } 1900 North 12th Street } } Philadelphia, PA 19122 } } jbs-at-temple.edu } } (215) 204 8839, fax (215) 204 0486 } } http://astro.temple.edu/~jbs
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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==============================Original Headers============================== 16, 35 -- From PhillipsT-at-missouri.edu Thu Jan 20 10:20:18 2011 16, 35 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KGKG9E014299 16, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:20:16 -0600 16, 35 -- X-IronPort-Anti-Spam-Filtered: true 16, 35 -- X-IronPort-Anti-Spam-Result: AvsEAH7vN03RauUp/2dsb2JhbACkUXO2AYheAoJuIoI+BIRviVmCcw 16, 35 -- Received: from um-nsmtpout1.um.umsystem.edu ([209.106.229.41]) 16, 35 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Jan 2011 10:20:14 -0600 16, 35 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 16, 35 -- Thu, 20 Jan 2011 10:19:55 -0600 16, 35 -- Received: from UM-MSXHUB05.um.umsystem.edu (131.151.121.205) by 16, 35 -- UM-THUB01.um.umsystem.edu (209.106.230.181) with Microsoft SMTP Server (TLS) 16, 35 -- id 8.2.254.0; Thu, 20 Jan 2011 10:19:55 -0600 16, 35 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 16, 35 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Thu, 20 Jan 2011 16, 35 -- 10:19:54 -0600 16, 35 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 16, 35 -- Date: Thu, 20 Jan 2011 10:19:53 -0600 16, 35 -- Subject: [Microscopy] Re: Microtome History 16, 35 -- Thread-Topic: [Microscopy] Re: Microtome History 16, 35 -- Thread-Index: Acu4ujnK/W+eYZMgQe2m/DmfMJG5qAAAyCyg 16, 35 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF1C4596EF46-at-UM-EMAIL05.um.umsystem.edu} 16, 35 -- References: {201101201553.p0KFrlKj031360-at-ns.microscopy.com} 16, 35 -- In-Reply-To: {201101201553.p0KFrlKj031360-at-ns.microscopy.com} 16, 35 -- Accept-Language: en-US 16, 35 -- Content-Language: en-US 16, 35 -- X-MS-Has-Attach: 16, 35 -- X-MS-TNEF-Correlator: 16, 35 -- acceptlanguage: en-US 16, 35 -- Content-Type: text/plain; charset="iso-8859-1" 16, 35 -- MIME-Version: 1.0 16, 35 -- X-OriginalArrivalTime: 20 Jan 2011 16:19:55.0726 (UTC) FILETIME=[E009CEE0:01CBB8BD] 16, 35 -- Content-Transfer-Encoding: 8bit 16, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KGKG9E014299 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 30 -- From prvs=994c3f914=John.Mardinly-at-wdc.com Thu Jan 20 12:33:11 2011 26, 30 -- Received: from wdscspam2.wdc.com (wdscspam2.wdc.com [129.253.55.43]) 26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KIX9hP006832 26, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 12:33:10 -0600 26, 30 -- X-IronPort-AV: E=Sophos;i="4.60,352,1291622400"; 26, 30 -- d="scan'208";a="172514723" 26, 30 -- Received: from unknown (HELO wdscexfe01.sc.wdc.com) ([172.25.19.1]) 26, 30 -- by wdscspam2.wdc.com with ESMTP; 20 Jan 2011 10:33:04 -0800 26, 30 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe01.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.4675); 26, 30 -- Thu, 20 Jan 2011 10:33:09 -0800 26, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 30 -- Content-class: urn:content-classes:message 26, 30 -- MIME-Version: 1.0 26, 30 -- Content-Type: text/plain; 26, 30 -- charset="us-ascii" 26, 30 -- Subject: RE: [Microscopy] Re: Microtome History 26, 30 -- Date: Thu, 20 Jan 2011 10:33:08 -0800 26, 30 -- Message-ID: {34061960C62E274C8AF1DCAA65655558097AA6D8-at-wdksjexbe01.msj.wdc.com} 26, 30 -- In-Reply-To: {201101201630.p0KGU7CT027262-at-ns.microscopy.com} 26, 30 -- X-MS-Has-Attach: 26, 30 -- X-MS-TNEF-Correlator: 26, 30 -- Thread-Topic: [Microscopy] Re: Microtome History 26, 30 -- Thread-Index: Acu4v1BKcpZtax5uRhqiCEWvlx7rtAAEQtjQ 26, 30 -- References: {201101201630.p0KGU7CT027262-at-ns.microscopy.com} 26, 30 -- From: "John Mardinly" {John.Mardinly-at-wdc.com} 26, 30 -- To: {PhillipsT-at-missouri.edu} 26, 30 -- Cc: {Microscopy-at-microscopy.com} 26, 30 -- X-OriginalArrivalTime: 20 Jan 2011 18:33:09.0168 (UTC) FILETIME=[7C804300:01CBB8D0] 26, 30 -- Content-Transfer-Encoding: 8bit 26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KIX9hP006832 ==============================End of - Headers==============================
One of the hospitals here in Honolulu had an old RCA TEM that had a rubber mallet in the alignment kit. They kept that old scope going until the early 1980s, I believe, with frequent visits from an aged service technician. It was a mechanical wonder.
I also heard of many of the early iterations of ultramicrotome, possibly at the knee of Caroline Schooley, from whom I learned TEM in 1976. One version I heard was mounting a block on the blade of a fan and running it past a stationary knife at high speed, using the position of venetian blinds on a sunny day as thickness control. One had to search around the room for the sections. I had to make knives from plate glass for a couple of years, and I might as well have given up on the pliers and just thrown the glass on the floor...
Aloha, Tina
Twenty to thirty foot waves predicted on the North Shore today.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 22 -- From tina-at-pbrc.hawaii.edu Thu Jan 20 12:34:02 2011 6, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KIY0GB009168 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 12:34:00 -0600 6, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p0KIXwu7025021 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:33:58 -1000 (HST) 6, 22 -- Received: from localhost (tina-at-localhost) 6, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p0KIXvLm025016 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:33:58 -1000 (HST) 6, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 6, 22 -- Date: Thu, 20 Jan 2011 08:33:57 -1000 (HST) 6, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 22 -- X-X-Sender: tina-at-b1000 6, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 22 -- Subject: Re: [Microscopy] Early microtomy--urban legend? 6, 22 -- In-Reply-To: {201101201744.p0KHi2wr017777-at-ns.microscopy.com} 6, 22 -- Message-ID: {Pine.GSO.4.64.1101200823400.24710-at-b1000} 6, 22 -- References: {201101201744.p0KHi2wr017777-at-ns.microscopy.com} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
If you check out the Glauert texts, in the volume by norma Reid and julian Beesley (pub 1991), they talk about the old high speed microtomes apparently razor blades were rotated at 10k rpm (the refs are Obrien et al 1943, and Fullam et al 1946, the Obrien paper was actually in Science vol 98, 455-456). For fun I made a similar device out of a dremel and a plastic salad bowl, the dremel cut the sections which flew off and stuck to the inner surface of the plastic bowl. I then filled it with water and the sections (at least some of them) floated off. The thickness was completely random, and the bowl size a bit enormous and not stationary.. think collecting sections from the surface of the Atlantic... but they were useable.. sort of... Obviously no serial sections. The other thing is that I was using contemporary resin technology, not available 65 years ago when this work was done, but I guess at the "cutting edge" (pun intended) S
Simon C. Watkins Ph.D, FRC Path Professor and Vice Chair Cell Biology and Physiology Professor Immunology Director Center for Biologic Imaging BSTS 225 University of Pittsburgh 3500 Terrace St Pittsburgh PA 15261 412-352-2277 www.cbi.pitt.edu
-----Original Message----- X-from: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] Sent: Thursday, January 20, 2011 12:37 PM To: Watkins, Simon C
Indeed. We are in a building that was often broken into. Years ago, I made it my business to collect the old door panes --they were a tinted glass, about 1/4" thick, and had just the right temper to make excellent knives.
Joel
On Thu, Jan 20, 2011 at 10:59 AM, {oshel1pe-at-cmich.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I know at least 2 old microscopists who made } glass knifes by breaking window glass - one of } them here at CMU. He went to construction sites } to get the broken windows. } } I'm running down the reference and (I hope) an } image, but one of the early tries at } ultramicrotomy was sticking razor blades on a } centrifuge rotor, then mount the sections on the } tub, close the lid and turn on the centrifuge. } The sections where then picked up from inside the } tub, having been flung willy-nilly around the } inside. } (The image is used in the microtomy lecture to } convince the students thin sectioning could be a } lot worse than they think it is.) } } Phil } } } Von: Muţ Wolfgang } } Gesendet: Donnerstag, 20. J"nner 2011 11:46 } } An: 'joelsheffield-at-gmail.com' } } Cc: 'microscopy-at-microscopy.com' } } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } Joel wrote: } } } Now, the urban legend.Ý } } I had heard that a group (unnamed) was having a } } vigorous discussion about the best way to strop } } a steel knife so as to get the best edge for } } cutting thin sections.Ý How many strokes, in } } which direction, which side of the blade, etc.Ý } } During the discussion, a bottle of Coke was } } knocked off the table and broke into many } } pieces.Ý } } The story goes that one, or another, of these } } people took up one of the shards, and suggested } } that it would make a good knife edge. } } Verification anyone? { } } } } Joel } } } } } } Good morning, } } Dear Joel, dear all } } } } I can't tell or verify the above mentioned } } "Coke-shard-story" but - indeed - I can add } } information as to the "knocked off } } table"-part.... } } } } I had always heared (personal informations from } } elder REICHERT-freaks and service people, from } } lectures/lecturers in the 70ies and 80ies as } } well as personally from H. Sitte himself) that } } the group around H. SITTE at REICHERT (now } } LEICA) in the late 1950ies/60ies was/were doing } } such experiments with {glass plates} .... which } } were thrown down to the floor... and one of them } } (it was Sitte himself, as he told me) picked up } } the shards evaluating the edges of "the most } } promising one" for sectioning purposes (who of } } that group had the "invention" to use glass as } } a} crude { knife (element) I unfortunately don't } } know. } } } } By the way, digging a little bit on this in } } Google, I found one hint on a document in a } } German data bank at } } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver-ffentlicht } } ("A simple ultramicrotome with thermal advance" published) } } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } } } Note:Ý {Naturwissenschaften} is the former title } } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } } Another source:Ý http://www.freepatentsonline.com/3828641.html: } } United States Patent US3828641: } } } } ==} Apparatus for adjusting the elevation of a } } specimen in microtomes, particularly } } ultramicrotomes } } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } } [as written in the patent document!]/Saar } } Germany) } } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } } Filed:Ý Nov. 10,1972 } } } } Also see } } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } } (From the book Michael J. Dykstra, Laura E. } } Reuss - 2003Ý (see page "History: in the text } } displayed } } the Reichert OMU-1 (before 1964, perhaps see } } document above in {Naturwissenschaften} 1955) } } from Sitte described as having thermal advance, } } Sitte was never happy with, since 1964: OMU-2 } } with thermal advance.... } } } } This just for your pleasure.... } } (and I sure that searching for Sitte Hellmuth or } } Sitte and microtome will find some results more } } on that interesting and exciting "history" of } } ultramicrotomy advances..... } } } } Best wishes and regards, } } have a good and successful rest of the week and a beautiful weekend, } } } } Wolfgang MUSS (Ph.D.) } } EM-Lab Gen.Hosp. SALK-LKH } } SALZBURG } } AUSTRIA } } } } } } } } } } } } } } } } } -----Urspr¸ngliche Nachricht----- } } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } } Gesendet: Donnerstag, 20. J"nner 2011 06:51 } } } An: Muţ Wolfgang } } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } } America } } } ToÝ Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } } } ----- } } } } } } It is certainly clear that there are many interesting stories --some } } } urban legends, and some insights into the minds of those that founded } } } the discipline of thin section electron microscopy. } } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } } microtome that I was told was developed by Sjostrand. } } } This thing consisted of a disk-like plate, about 4-5 inches in } } } diameter, with an eccentrically mounted arm that stuck out of the } } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } } The plate itself was mounted in a frame and lubricated with oil --the } } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } } on the wall by a long V belt (to minimize vibration), and the entire } } } plate rotated, bringing the block past the knife on each rotation.Ý The } } } sections that resulted tended to be arc shaped.Ý We got it working, but } } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } } remembers this type of machine -- assuming that my description is } } } comprehensible. } } } } } } The other microtome that I used, and really admire, was the Huxley } } } microtome, originally sold by Cambridge, and ultimately marketed by } } } LKB.Ý Although it used a mechanical advance, all of the movements of } } } the cutting arm were made by bending sheets of spring steel, so that } } } there were no surfaces that moved against each other.Ý Moreover, the } } } cutting stroke was controlled by an oil-filled damper, to minimize } } } chatter. -- a remarkable and stable machine. } } } } } } Now, the urban legend. } } } I had heard that a group (unnamed) was having a vigorous discussion } } } about the best way to strop a steel knife so as to get the best edge } } } for cutting thin sections.Ý How many strokes, in which direction, which } } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } } knocked off the table and broke into many pieces. } } } The story goes that one, or another, of these people took up one of the } } } shards, and suggested that it would make a good knife edge. } } } Verification anyone? } } } } } } Joel } } } } } } -- } } } Joel B. Sheffield, Ph.D. } } } Biology Department, Temple University } } } 1900 North 12th Street } } } Philadelphia, PA 19122 } } } jbs-at-temple.edu } } } (215) 204 8839, fax (215) 204 0486 } } } http://astro.temple.edu/~jbs } } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } (989) 774-3576 } } } ==============================Original Headers============================== } 6, 27 -- From oshel1pe-at-cmich.edu Thu Jan 20 09:53:21 2011 } 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFrLZ3030383 } 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:53:21 -0600 } 6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0KFrBHA005464 } 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:53:20 -0500 } 6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); } 6, 27 -- Thu, 20 Jan 2011 10:53:17 -0500 } 6, 27 -- Mime-Version: 1.0 } 6, 27 -- Message-Id: {a06240802c95e09c0c794-at-[141.209.160.249]} } 6, 27 -- In-Reply-To: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} } 6, 27 -- References: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} } 6, 27 -- Date: Thu, 20 Jan 2011 10:53:12 -0500 } 6, 27 -- To: Microscopy-at-microscopy.com } 6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } 6, 27 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" } 6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 6, 27 -- X-OriginalArrivalTime: 20 Jan 2011 15:53:17.0197 (UTC) FILETIME=[273DA3D0:01CBB8BA] } 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) } 6, 27 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001:-0.5) } 6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 } 6, 27 -- X-CanItPRO-Stream: default } 6, 27 -- X-Canit-Stats-ID: 02DVPRkyE - 9b55b8297af8 - 20110120 } 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 } 6, 27 -- Content-Transfer-Encoding: 8bit } 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KFrLZ3030383 } ==============================End of - Headers============================== }
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
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==============================Original Headers============================== 17, 27 -- From swatkins-at-pitt.edu Thu Jan 20 13:01:00 2011 17, 27 -- Received: from exprod7og117.obsmtp.com (exprod7og117.obsmtp.com [64.18.2.6]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0KJ0xnS017907 17, 27 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 13:00:59 -0600 17, 27 -- Received: from source ([136.142.251.42]) (using TLSv1) by exprod7ob117.postini.com ([64.18.6.12]) with SMTP 17, 27 -- ID DSNKTTiGao0K4p6etfxldeCwf8x0pHNvrjEz-at-postini.com; Thu, 20 Jan 2011 11:00:59 PST 17, 27 -- Received: from pitt-exch-14.univ.pitt.edu ([169.254.1.248]) by 17, 27 -- pitt-ht-04.univ.pitt.edu ([136.142.251.42]) with mapi; Thu, 20 Jan 2011 17, 27 -- 14:00:57 -0500 17, 27 -- From: "Watkins, Simon C" {swatkins-at-pitt.edu} 17, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 17, 27 -- Date: Thu, 20 Jan 2011 14:00:48 -0500 17, 27 -- Subject: RE: [Microscopy] Microtome History - was "Ernst Ruska" 17, 27 -- Thread-Topic: [Microscopy] Microtome History - was "Ernst Ruska" 17, 27 -- Thread-Index: Acu4yLELPf6IttVpQ0yllQgruK+LSwACk0SQ 17, 27 -- Message-ID: {F53DA84501B039439893BE88F0F963410FFCAB6D8D-at-PITT-EXCH-14.univ.pitt.edu} 17, 27 -- References: {201101201737.p0KHbH2S007671-at-ns.microscopy.com} 17, 27 -- In-Reply-To: {201101201737.p0KHbH2S007671-at-ns.microscopy.com} 17, 27 -- Accept-Language: en-US 17, 27 -- Content-Language: en-US 17, 27 -- X-MS-Has-Attach: 17, 27 -- X-MS-TNEF-Correlator: 17, 27 -- acceptlanguage: en-US 17, 27 -- Content-Type: text/plain; charset="iso-8859-1" 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Transfer-Encoding: 8bit 17, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KJ0xnS017907 ==============================End of - Headers==============================
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This whole discussion has been a "this is my life" for me. Sorry, Tina - the fan thing wasn't mine. I went to work for Dorothy Pitelka at U.C. Berkeley in '53, and our first attempts at thin sections were with a metal-knife Spencer microtome with a modifying wedge in the advance mechanism. Miserable. Then a MT-1 with glass knives; a local window company made 1" strips from old windows for us.
The rubber mallet was used on RCA EMU-2 scopes to align the objective aperture....
Caroline -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America 45301 Caspar Point Road, Box 117 Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 4, 18 -- From schooley-at-mcn.org Thu Jan 20 13:05:12 2011 4, 18 -- Received: from smtp2.mcn.org (smtp2.mcn.org [216.150.240.86]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KJ5Bml023547 4, 18 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 13:05:12 -0600 4, 18 -- Received: from [66.81.51.194] 4, 18 -- by smtp2.mcn.org with esmtpa (Exim 4.63) 4, 18 -- (envelope-from {schooley-at-mcn.org} ) 4, 18 -- id 1Pfzoi-0005EJ-6P; Thu, 20 Jan 2011 11:05:09 -0800 4, 18 -- Mime-Version: 1.0 4, 18 -- Message-Id: {a06200700c95e34089b6e-at-[66.81.41.32]} 4, 18 -- In-Reply-To: {201101201842.p0KIg3QP006164-at-ns.microscopy.com} 4, 18 -- References: {201101201842.p0KIg3QP006164-at-ns.microscopy.com} 4, 18 -- Date: Thu, 20 Jan 2011 11:05:00 -0800 4, 18 -- To: tina-at-pbrc.hawaii.edu 4, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 4, 18 -- Subject: [Microscopy] Re: Early microtomy--urban legend? 4, 18 -- Cc: {microscopy-at-microscopy.com} 4, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Paul Hazelton's comments about knowing someone who claimed to have used a rubber mallet to align a Siemens microscope, reminds me of my own experience along that line. I took mu first electron micrographs on an RCA EMB microscope in Robley Williams lab in the Physics Department here at the University of Michigan. This was only the second EM manufactured and sold by RCA. Robley had two of these instruments, one which he reserved for his own use and one that he allowed other 'qualified' persons to use. It was said that he selected the best pole pieces and power supplies from the two instruments and incorporated them to his own, and so he had one of the best electron microscopes in the country at the time. I was deemed a qualified user because I was studying under Professor L. O. Brockway who was internationally famous for his work on determining the structures of organic molecules by electron diffraction. Robley was, of course, probably best known for developing the method of shadow casting that was extensively used to enhance contrast in micrographs of particulate materials and surface replicas. At that time the tobacco mosaic virus, which have a beautiful long rod-like structure, was a favorite specimen to use to demonstrate the resolution of an EM. I remember a lecture on the merits of shadow casting where Robley showed a micrograph of beautifully shadowed long rod-like particles that everyone naturally assumed were tobacco mosaic virus; then Robley showed a second picture at higher magnification in which the particles could be clearly seen to be Lucky Strike cigarettes.
Anyway, halfway through the days of my graduate study Robley left the U of M, and his microscope was no longer available. To continue our work Professor Brockway managed to arrange for me to us an RCA EM2 electron microscope in the laboratory of Professor Thomas Francis in the School of Public Health (Prof Francis was famous for heading up the studies that confirmed the effectiveness of the Salk polio vaccine.) This instrument was a considerable improvement over the EMB; however, here is where the hammer-for-alignment feature came in. The condenser aperture on this model instrument did not have external controls for centering the condenser aperture, The aperture was merely a circular platinum disk with a small hole in the middle that was mounted in a cavity in the top of the condenser pole piece, and held in place with a retaining screw. Initially, the aperture had to be centered by trial and error, which involved disassembling the top of the column, removing the pole piece from the condenser lens, moving the aperture a bit, and then reassembling the instrument. To get a really good alignment of the aperture usually required several cycles of this routine combined with considerable luck. It was thus very tempting to work with dirty condenser apertures. FINALLY, however, someone (and I don't know that I ever heard who the sainted individual was) discovered that if the retaining screw was not tightened securely, so that the aperture could move around a bit, the aperture could be centered by tapping gently on the outside of the column. I don't recall ever using a rubber mallet, but the end of the handle of a screw driver did the job very well. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 2, 15 -- From bigelow-at-umich.edu Thu Jan 20 13:42:35 2011 2, 15 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 2, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KJgZBh018089 2, 15 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 13:42:35 -0600 2, 15 -- Received: FROM [76.234.170.127] (adsl-76-234-170-127.dsl.sfldmi.sbcglobal.net [76.234.170.127]) 2, 15 -- By hackers.mr.itd.umich.edu ID 4D38902A.A6C7B.20599 ; 2, 15 -- Authuser bigelow; 2, 15 -- 20 Jan 2011 14:42:34 EST 2, 15 -- Mime-Version: 1.0 2, 15 -- Message-Id: {p06240800c95e34c677dc-at-[76.226.70.137]} 2, 15 -- Date: Thu, 20 Jan 2011 14:42:08 -0500 2, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 2, 15 -- From: Wil Bigelow {bigelow-at-umich.edu} 2, 15 -- Subject: [Microscopy]RE:Early Micro 2, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Dr. Mardinly's comments about using a hammer to dislodge whiskers inside an ion pump leads me to point out that if the whiskers are not too firmly established it is often possible to get rid of them by turning the high voltage supply on for a few seconds three or four times with the pressure in the pump above 1 Pa (10-2 Torr), If this approach works it is gentler, and quieter, than the hammer method. Incidentally, this method (but not the hammer method) and other characteristics of ion pumps, are described on page 295 of my book, 'Vacuum Methods in Electron Microscopy'.
Good luck! -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 2, 15 -- From bigelow-at-umich.edu Thu Jan 20 14:05:08 2011 2, 15 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.12.86]) 2, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KK58DA002555 2, 15 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 14:05:08 -0600 2, 15 -- Received: FROM [76.234.170.127] (adsl-76-234-170-127.dsl.sfldmi.sbcglobal.net [76.234.170.127]) 2, 15 -- By tombraider.mr.itd.umich.edu ID 4D389573.7D8CA.363 ; 2, 15 -- Authuser bigelow; 2, 15 -- 20 Jan 2011 15:05:07 EST 2, 15 -- Mime-Version: 1.0 2, 15 -- Message-Id: {p06240802c95e4334d9d3-at-[76.234.170.127]} 2, 15 -- Date: Thu, 20 Jan 2011 15:04:41 -0500 2, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 2, 15 -- From: Wil Bigelow {bigelow-at-umich.edu} 2, 15 -- Subject: Hammering ion pumps 2, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Frank, it is good to see someone putting some thought into the process. I have had numerous students/clients come to me and ask for an analysis with some particular parameters only for me to ask if they really wanted to invest that much time and money. (Maybe I shouldn't have asked. I could have just given them the bill telling them that I only did what they asked. Naahh.)
Your interaction volume estimate seems reasonable, but you may need to look into cutting your voltage to cut down the volume. I would think 15 kV or even 12 kV would still get you a good response for most K-lines and would really cut down the volume.
Like Bill said, you want to match the volume to the feature size. That is the bottom line.
You probably also want to match the pixel size to the volume, but there is something to be said for oversampling. Even though the volume will be broader than your pixel, a lot of the interaction helps as the core. Yes, you will get stray signals from the surrounding area. You won't be able to say that the volume does not contain this element or that, but you will stand a better chance at finding what is elevated at that location as opposed to stepping over a feature and missing it.
Now as to your specific numbers, let me examine them.
You say that your pixel size is 0.8 um and there are 1024 across the image, so your field of view is roughly 800 um. I generally calculate magnification against a standard width of 120 mm (the width of a Polaroid print). That gives me a magnification of 150x. Now, I suppose if your image was displayed 560 mm wide on a 22-inch wide screen, it would be accurate to state it was at 700x magnification. Otherwise, there may be something wrong in your setup. It would be worth checking. However, your calculations of 2.3 um pixels with 512 of them across a 100x image does make mathematical sense.
(BTW, I am an old fogey in wanting to reference everything to a 5-inch Polaroid print. It doesn't matter if it's a thumbnail of an image on the web or in a journal or if it's plastered across a billboard. I tend to count all pictures as 5 inches wide in my mind's eye. Therefore, I have my SEM setup to calculate that way.)
I would probably setup for about 0.5 to 1.0 um steps across the boundaries. I would cut that even finer at lower voltages. (Indeed, I have such a sample in my queue.) I don't normally setup my pixels as my step sizes. They don't have to match unless you are collecting a spectral image or an x-ray map. Then I would want to match my pixel to my interaction volume or a bit smaller to make sure I have sampled everything well when in hunting mode.
Spectral images or x-ray maps do take considerably more time to collect. In that sense, I am with Bill to recommend that you chose your points wisely. You could probably collect good data much faster. I am not against elemental maps. Sometimes they are the best way to make a point, but they usually require pretty marked changes in concentration to perceive a change in intensity. For general work, I restrict myself to manually placed points or to a small raster of points in a specific region of interest.
Not knowing how your precipitates (might) occur, I can't give you a particular recommendation. I would be imaging as well as I could, probably in BSE mode, looking for visible signs of the particles and then using EDS to identify and quantify the material.
Dear Frank, The key is that you're "looking for slight changes in element concentrations at grain boundaries and precipitates." In order best to see these changes, the pixel size should be smaller than the features--half the feature size guarantees that at least one pixel will be entirely within the feature. If the pixel size is too large than you will measure the average element concentrations over both the feature and the surrounding area, which will make the apparent changes smaller than they really are. I would suggest taking an image to locate the features, then place the beam on each feature in turn to do the analysis. Also take a spectrum of the area next to each feature. This should give you the best chance of seeing differences and minimize the amount of data. Yours, Bill from: Frank_Karl-at-lincolnelectric.com
I've been thinking about EDS line scans and I'm attempting to maximize The data I collect.
I run at 20 KV on iron samples so my electron interaction range is between 1.5 to 2.3um (Berthe or K-O range calculations). According to data provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um at 700x. So right away I see if each image pixel represents a data point, I'm sampling about 2 pixel volumes per data point. Looking for a sudden and sharp change in element concentration seem difficult, but wait there's more. Let us introduce another plot complication.
My customers don't want to scan the entire image, tooo much data!
So I scan a smaller section of image described above and instruct the computer to take 512 data points along a line that might only be 400 (visual estimate from screen) image pixels long.
So... Should I reduce the image resolution so that each image pixel will be one data point and scan the entire image? Would it be better to lower the magnification so the image pixels are larger (say 1.2um ). I could go to 100x and 512 horizontal image pixel so each image pixel is 2.3um?
I'm looking for slight changes in element concentration at grain boundaries and precipitates and of course I want to produce the most meaningful data possible. I'm running at conditions that suggest my electron interactive volume is larger than my spatial resolution as to produce "continuous" data.
Or am I just over thinking the process?
Any suggestion or comments would be welcome
Frank Lincoln Electric Company -- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 11, 17 -- From frank_karl-at-lincolnelectric.com Wed Jan 19 07:37:23 2011 11, 17 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JDbNoO031503 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 07:37:23 -0600 11, 17 -- Subject: EDS line scans and time on my hands 11, 17 -- To: Microscopy-at-microscopy.com 11, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 11, 17 -- Message-ID: {OF4A811DE5.D47D1408-ON8525781D.004A569D-8525781D.004AD372-at-lincolnelectric.com} 11, 17 -- From: Frank_Karl-at-lincolnelectric.com 11, 17 -- Date: Wed, 19 Jan 2011 08:38:03 -0500 11, 17 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 11, 17 -- 01/19/2011 08:37:18 AM 11, 17 -- MIME-Version: 1.0 11, 17 -- Content-Type: text/plain; 11, 17 -- charset="UTF-8" 11, 17 -- Content-Transfer-Encoding: 8bit 11, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p0JDbNoO031503 ==============================End of - Headers==============================
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Hello Dr. Zhou,
Our research group has also struggled with the challenge of obtaining quality diffraction patterns from fine precipitates. The problem is magnified (no pun intended) when the precipitates are very small, less than 5nm in diameter, or when there is a high concentration of precipitates with potentially different chemistry and crystal structures (as is often the case with carbon replicas). The latter problem prohibits the use of selected area diffraction techniques for identifying individual precipitates as you will collect information from many different precipitates with even the smallest SA aperture. I'm only slightly surprised that you haven't been able to achieve good results using nano beam diffraction. We've found that vanadium and chromium-rich carbides are rapidly degraded by the electron beam. The nano beam or convergent beam probe tends to damage the crystal structure of the carbides as soon as the probe touches the precipitate. We're not sure whether radiolysis or knock-on damage is the main culprit since we've obtained less than ideal results at both 80kV and at 200kV. We have resorted to imaging the precipitates with HRTEM and interpreting the FFTs to determine structure but we're still making some attempts to use convergent beam probes to more rapidly acquire diffraction patterns.
Good luck and keep us updated of your successes, Chris Drexel University - Dynamic Characterization Group
On Wed, Jan 19, 2011 at 3:47 PM, {z.zhou-at-lboro.ac.uk} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: z.zhou-at-lboro.ac.uk } Name: Zhou } } Organization: Dept of Materials, Loughborough University, UK } } Title-Subject: [Filtered] TEM diffraction 3nm precipitates } } Message: Greetings listers, } } Iím studying crystal structure of some } carbides/nitrides precipitates in steel. The } precipitates are 2-5nm size in a C replica TEM } specimen, V-, Cr-, and Nb-rich by STEM/EDX. } } How can I get electron diffraction or crystal } structure information of these fine precipitates? } } I tried on a TecnaiF20 TEM using nanoprobe spot } 7, focused on a small particle, then diffraction, } most of the time I got amorphous rings of C } support, no obvious diffraction discs. STEM mode } did indicate some C contamination on the session. } Assuming minimal C contamination, is it possible } that this method can give me some sort of } convergence beam electron diffraction pattern, } which may help me determine the crystal structure } of the precipitates? } } Iím also open to other methods. } } Your advice is highly appreciated. } } Zhou } } Dr Zhaoxia Zhou } Dept of Materials } Loughborough University } Loughborough, UK } LE11 3TU } } } Login Host: 158.125.68.189 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 15, 24 -- From zaluzec-at-microscopy.com Wed Jan 19 14:33:42 2011 } 15, 24 -- Received: from znl.com ([206.69.208.20]) } 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JKXf70014726 } 15, 24 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 14:33:42 -0600 } 15, 24 -- Received: from localhost (localhost [127.0.0.1]) } 15, 24 -- by znl.com (Postfix) with ESMTP id E7C66316930 } 15, 24 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 14:33:41 -0600 (CST) } 15, 24 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 15, 24 -- Received: from znl.com ([127.0.0.1]) } 15, 24 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 15, 24 -- with ESMTP id yEihmXkByYx9 for {microscopy-at-microscopy.com} ; } 15, 24 -- Wed, 19 Jan 2011 14:33:41 -0600 (CST) } 15, 24 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 15, 24 -- by znl.com (Postfix) with ESMTPA id 6BC61316925 } 15, 24 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 14:33:41 -0600 (CST) } 15, 24 -- Mime-Version: 1.0 } 15, 24 -- Message-Id: {p06240802c95cfb015564-at-[206.69.208.22]} } 15, 24 -- Date: Wed, 19 Jan 2011 14:33:39 -0600 } 15, 24 -- To: microscopy-at-microscopy.com } 15, 24 -- From: z.zhou-at-lboro.ac.uk (by way of MicroscopyListserver) } 15, 24 -- Subject: viaWWW: TEM diffraction 3nm precipitates } 15, 24 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 15, 24 -- Content-Transfer-Encoding: 8bit } 15, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JKXf70014726 } ==============================End of - Headers============================== }
-- Christopher Winkler Dynamic Characterization Group http://www.materials.drexel.edu/dcg/ Drexel University 267-496-0587
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I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf).
I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution.
The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses.
Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 6, 37 -- From kraftpiano-at-gmail.com Thu Jan 20 23:08:26 2011 6, 37 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L58Q25003006 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 23:08:26 -0600 6, 37 -- Received: by yxl31 with SMTP id 31so469145yxl.0 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 21:08:26 -0800 (PST) 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 37 -- d=gmail.com; s=gamma; 6, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding 6, 37 -- :subject:date:message-id:to:mime-version:x-mailer; 6, 37 -- bh=0UOwJelpZ6QJtvR6h504fJNBtAxqg11VNSbyg5wJQ74=; 6, 37 -- b=CKvCVRNoVTsFwRnLpU5lCXf8YvPMxH1N3dS7ys8UPf3yiqhPDWGbMo0obmG88cgOgo 6, 37 -- XZULU3FfCYHsarDBM9jexEC0W3v5YP7EFDMCVYhQlXG/1kA7Yhd73anJHaX3ZIz+H7uz 6, 37 -- JgDVe+ofiVTeCAAmXGLSf7iUKv6XvcnYIVDww= 6, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 37 -- d=gmail.com; s=gamma; 6, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id 6, 37 -- :to:mime-version:x-mailer; 6, 37 -- b=PR17AmFsaRKhUKvApaUTF9Rk1PZybTM2uGPbnyI+JSfekwjDyixYQTfQWeBGOPBeUR 6, 37 -- jtKbSKxwwTpYoyFNLlhFZrNVcU8rUujb+K2L3feJgIONXADzcGx34Xj28IMbBnWZHLwQ 6, 37 -- 1EKfQrQ/cpSgDSCYg9bEB0VVr0WIDudjYmUZI= 6, 37 -- Received: by 10.151.68.12 with SMTP id v12mr198668ybk.387.1295586505917; 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) 6, 37 -- Received: from [10.101.1.3] (c-98-249-167-116.hsd1.fl.comcast.net [98.249.167.116]) 6, 37 -- by mx.google.com with ESMTPS id u10sm82704yba.13.2011.01.20.21.08.24 6, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) 6, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} 6, 37 -- Content-Type: text/plain; charset=us-ascii 6, 37 -- Subject: STM tip advice. 6, 37 -- Date: Fri, 21 Jan 2011 00:08:23 -0500 6, 37 -- Message-Id: {EA4CFA28-451C-4E4D-A543-EEA026824B89-at-gmail.com} 6, 37 -- To: microscopy-at-microscopy.com 6, 37 -- Mime-Version: 1.0 (Apple Message framework v1082) 6, 37 -- X-Mailer: Apple Mail (2.1082) 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0L58Q25003006 ==============================End of - Headers==============================
Justin: I used to make my own probe tips (for poking around inside an IC) with tungsten wire and KOH. We had a set-up under a microscope that applied a voltage (I think it was 5 volts and an amp or so but don't remember exactly) between the wire and the KOH (in a metal container) and you moved the wire in and out of the KOH to form the tip. The surface tension of the KOH as the wire moves in and out forms a nice pointy tip. It's best to have something that controls the position and movement of the wire mechanically unless you have very steady hands. Welding the wire to the KOH container at 10-20X magnification will you make you jump!
On 1/20/11 11:08 PM, kraftpiano-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf). } } I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution. } } The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses. } } Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway. } } Thanks, } } Justin A. Kraft } } ==============================Original Headers============================== } 6, 37 -- From kraftpiano-at-gmail.com Thu Jan 20 23:08:26 2011 } 6, 37 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) } 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L58Q25003006 } 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 23:08:26 -0600 } 6, 37 -- Received: by yxl31 with SMTP id 31so469145yxl.0 } 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 21:08:26 -0800 (PST) } 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 37 -- d=gmail.com; s=gamma; } 6, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding } 6, 37 -- :subject:date:message-id:to:mime-version:x-mailer; } 6, 37 -- bh=0UOwJelpZ6QJtvR6h504fJNBtAxqg11VNSbyg5wJQ74=; } 6, 37 -- b=CKvCVRNoVTsFwRnLpU5lCXf8YvPMxH1N3dS7ys8UPf3yiqhPDWGbMo0obmG88cgOgo } 6, 37 -- XZULU3FfCYHsarDBM9jexEC0W3v5YP7EFDMCVYhQlXG/1kA7Yhd73anJHaX3ZIz+H7uz } 6, 37 -- JgDVe+ofiVTeCAAmXGLSf7iUKv6XvcnYIVDww= } 6, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 6, 37 -- d=gmail.com; s=gamma; } 6, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id } 6, 37 -- :to:mime-version:x-mailer; } 6, 37 -- b=PR17AmFsaRKhUKvApaUTF9Rk1PZybTM2uGPbnyI+JSfekwjDyixYQTfQWeBGOPBeUR } 6, 37 -- jtKbSKxwwTpYoyFNLlhFZrNVcU8rUujb+K2L3feJgIONXADzcGx34Xj28IMbBnWZHLwQ } 6, 37 -- 1EKfQrQ/cpSgDSCYg9bEB0VVr0WIDudjYmUZI= } 6, 37 -- Received: by 10.151.68.12 with SMTP id v12mr198668ybk.387.1295586505917; } 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) } 6, 37 -- Received: from [10.101.1.3] (c-98-249-167-116.hsd1.fl.comcast.net [98.249.167.116]) } 6, 37 -- by mx.google.com with ESMTPS id u10sm82704yba.13.2011.01.20.21.08.24 } 6, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); } 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) } 6, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} } 6, 37 -- Content-Type: text/plain; charset=us-ascii } 6, 37 -- Subject: STM tip advice. } 6, 37 -- Date: Fri, 21 Jan 2011 00:08:23 -0500 } 6, 37 -- Message-Id: {EA4CFA28-451C-4E4D-A543-EEA026824B89-at-gmail.com} } 6, 37 -- To: microscopy-at-microscopy.com } 6, 37 -- Mime-Version: 1.0 (Apple Message framework v1082) } 6, 37 -- X-Mailer: Apple Mail (2.1082) } 6, 37 -- Content-Transfer-Encoding: 8bit } 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0L58Q25003006 } ==============================End of - Headers============================== }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 214-567-0360 SC Packaging FA Texas Instruments, Inc. 13536 N. Central Expressway MS 940 Dallas, TX 75243 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 4, 23 -- From r-holdford-at-ti.com Fri Jan 21 02:06:37 2011 4, 23 -- Received: from bear.ext.ti.com (bear.ext.ti.com [192.94.94.41]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L86bjR023049 4, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 02:06:37 -0600 4, 23 -- Received: from dlep33.itg.ti.com ([157.170.170.112]) 4, 23 -- by bear.ext.ti.com (8.13.7/8.13.7) with ESMTP id p0L86ZUd007341 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 23 -- Fri, 21 Jan 2011 02:06:35 -0600 4, 23 -- Received: from [156.117.194.82] (localhost [127.0.0.1]) 4, 23 -- by dlep33.itg.ti.com (8.13.7/8.13.7) with ESMTP id p0L86Zrf004655; 4, 23 -- Fri, 21 Jan 2011 02:06:35 -0600 (CST) 4, 23 -- Message-ID: {4D393E8B.4010507-at-ti.com} 4, 23 -- Date: Fri, 21 Jan 2011 02:06:35 -0600 4, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 23 -- Organization: SC Packaging MPI Team 4, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 4, 23 -- MIME-Version: 1.0 4, 23 -- To: kraftpiano-at-gmail.com, MSA Listserver {Microscopy-at-microscopy.com} 4, 23 -- Subject: Re: [Microscopy] STM tip advice. 4, 23 -- References: {201101210508.p0L58XuH003221-at-ns.microscopy.com} 4, 23 -- In-Reply-To: {201101210508.p0L58XuH003221-at-ns.microscopy.com} 4, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello, One of my friends told me that in the sixties, he and his colleagues were looking for decommissioned broken black glass covers of gravestones. According to him, it was the best material for glass knives.
Oldrich
On Thursday 20 of January 2011 16:54:51 you wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } I know at least 2 old microscopists who made } glass knifes by breaking window glass - one of } them here at CMU. He went to construction sites } to get the broken windows. } } I'm running down the reference and (I hope) an } image, but one of the early tries at } ultramicrotomy was sticking razor blades on a } centrifuge rotor, then mount the sections on the } tub, close the lid and turn on the centrifuge. } The sections where then picked up from inside the } tub, having been flung willy-nilly around the } inside. } (The image is used in the microtomy lecture to } convince the students thin sectioning could be a } lot worse than they think it is.) } } Phil } } } Von: Muţ Wolfgang } } Gesendet: Donnerstag, 20. J”nner 2011 11:46 } } An: 'joelsheffield-at-gmail.com' } } Cc: 'microscopy-at-microscopy.com' } } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } } Joel wrote: } } } Now, the urban legend.Ý } } } } I had heard that a group (unnamed) was having a } } vigorous discussion about the best way to strop } } a steel knife so as to get the best edge for } } cutting thin sections.Ý How many strokes, in } } which direction, which side of the blade, etc.Ý } } During the discussion, a bottle of Coke was } } knocked off the table and broke into many } } pieces.Ý } } The story goes that one, or another, of these } } people took up one of the shards, and suggested } } that it would make a good knife edge. } } Verification anyone? { } } } } Joel } } } } } } Good morning, } } Dear Joel, dear all } } } } I can't tell or verify the above mentioned } } "Coke-shard-story" but - indeed - I can add } } information as to the "knocked off } } table"-part.... } } } } I had always heared (personal informations from } } elder REICHERT-freaks and service people, from } } lectures/lecturers in the 70ies and 80ies as } } well as personally from H. Sitte himself) that } } the group around H. SITTE at REICHERT (now } } LEICA) in the late 1950ies/60ies was/were doing } } such experiments with {glass plates} .... which } } were thrown down to the floor... and one of them } } (it was Sitte himself, as he told me) picked up } } the shards evaluating the edges of "the most } } promising one" for sectioning purposes (who of } } that group had the "invention" to use glass as } } a} crude { knife (element) I unfortunately don't } } know. } } } } By the way, digging a little bit on this in } } Google, I found one hint on a document in a } } German data bank at } } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver–ffentlicht } } ("A simple ultramicrotome with thermal advance" published) } } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } } } Note:Ý {Naturwissenschaften} is the former title } } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } } Another source:Ý http://www.freepatentsonline.com/3828641.html: } } United States Patent US3828641: } } } } ==} Apparatus for adjusting the elevation of a } } specimen in microtomes, particularly } } ultramicrotomes } } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } } [as written in the patent document!]/Saar } } Germany) } } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } } Filed:Ý Nov. 10,1972 } } } } Also see } } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+a } } nd+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8 } } &hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ve } } d=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false (From the } } book Michael J. Dykstra, Laura E. } } Reuss - 2003Ý (see page "History: in the text } } displayed } } the Reichert OMU-1 (before 1964, perhaps see } } document above in {Naturwissenschaften} 1955) } } from Sitte described as having thermal advance, } } Sitte was never happy with, since 1964: OMU-2 } } with thermal advance.... } } } } This just for your pleasure.... } } (and I sure that searching for Sitte Hellmuth or } } Sitte and microtome will find some results more } } on that interesting and exciting "history" of } } ultramicrotomy advances..... } } } } Best wishes and regards, } } have a good and successful rest of the week and a beautiful weekend, } } } } Wolfgang MUSS (Ph.D.) } } EM-Lab Gen.Hosp. SALK-LKH } } SALZBURG } } AUSTRIA } } } } } -----Urspr¸ngliche Nachricht----- } } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } } Gesendet: Donnerstag, 20. J”nner 2011 06:51 } } } An: Muţ Wolfgang } } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } } America } } } ToÝ Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } } } ----- } } } } } } It is certainly clear that there are many interesting stories --some } } } urban legends, and some insights into the minds of those that founded } } } the discipline of thin section electron microscopy. } } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } } microtome that I was told was developed by Sjostrand. } } } This thing consisted of a disk-like plate, about 4-5 inches in } } } diameter, with an eccentrically mounted arm that stuck out of the } } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } } The plate itself was mounted in a frame and lubricated with oil --the } } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } } on the wall by a long V belt (to minimize vibration), and the entire } } } plate rotated, bringing the block past the knife on each rotation.Ý The } } } sections that resulted tended to be arc shaped.Ý We got it working, but } } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } } remembers this type of machine -- assuming that my description is } } } comprehensible. } } } } } } The other microtome that I used, and really admire, was the Huxley } } } microtome, originally sold by Cambridge, and ultimately marketed by } } } LKB.Ý Although it used a mechanical advance, all of the movements of } } } the cutting arm were made by bending sheets of spring steel, so that } } } there were no surfaces that moved against each other.Ý Moreover, the } } } cutting stroke was controlled by an oil-filled damper, to minimize } } } chatter. -- a remarkable and stable machine. } } } } } } Now, the urban legend. } } } I had heard that a group (unnamed) was having a vigorous discussion } } } about the best way to strop a steel knife so as to get the best edge } } } for cutting thin sections.Ý How many strokes, in which direction, which } } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } } knocked off the table and broke into many pieces. } } } The story goes that one, or another, of these people took up one of the } } } shards, and suggested that it would make a good knife edge. } } } Verification anyone? } } } } } } Joel } } } } } } -- } } } Joel B. Sheffield, Ph.D. } } } Biology Department, Temple University } } } 1900 North 12th Street } } } Philadelphia, PA 19122 } } } jbs-at-temple.edu } } } (215) 204 8839, fax (215) 204 0486 } } } http://astro.temple.edu/~jbs
==============================Original Headers============================== 4, 23 -- From benada-at-biomed.cas.cz Fri Jan 21 03:07:43 2011 4, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L97gOW007522 4, 23 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 03:07:43 -0600 4, 23 -- Received: from u117ob.localnet (a100ix.mbu.cas.cz [147.231.44.171]) 4, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 4, 23 -- (No client certificate requested) 4, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id D06561A446DE 4, 23 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 10:07:41 +0100 (CET) 4, 23 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 4, 23 -- Organization: =?utf-8?q?Mikrobiologck=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?= 4, 23 -- To: microscopy-at-microscopy.com 4, 23 -- Subject: Re: [Microscopy] Re: Microtome History - was "Ernst Ruska" 4, 23 -- Date: Fri, 21 Jan 2011 10:07:31 +0100 4, 23 -- User-Agent: KMail/1.13.5 (Linux/2.6.32-5-686; KDE/4.4.5; i686; ; ) 4, 23 -- References: {201101201554.p0KFspb2001352-at-ns.microscopy.com} 4, 23 -- In-Reply-To: {201101201554.p0KFspb2001352-at-ns.microscopy.com} 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Type: Text/Plain; 4, 23 -- charset="windows-1250" 4, 23 -- Message-Id: {201101211007.31576.benada-at-biomed.cas.cz} 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0L97gOW007522 ==============================End of - Headers==============================
All I have been watching the advice on this problem and whilst I agree with all that has been said may I offer a little more?
I have had experience with materials that forced us to use cryo within an investigation, then we (the client and I) purchased an early FEGSEM. As you may guess the cryo kit would not fit onto the FEG! Forced to take another route we found that being very careful in the FEG we could work on the very sensitive material without any apparent problems.
The settings we used were those typical for sensitive materials:-
1. Coat the specimen with a minimal current and an extended working distance e.g. normal would be ~5cm we used 7cm from memory(?) 2. Use a low emission current. 3. Use a spot size/probe current near the lowest limit of the instrument. 4. Use the out of lens detector for minimal charge (~15mm WD)
I hope that this may help?
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: 20 January 2011 17:58 To: protrain-at-emcourses.com
Dear All, I had the same problem visualizing bee wax structures and not owning an ESEM ;-)
Solution: I put the structure on a large specimen mount, putting 2 bands of conductive adhesive tape left and right on top and put it in my normal Pt-sputtercoater. Concerning the heat during sputtering, I took off the sputter-head and added a second glass vessel (I took it from my carbon-coater) on top of the first. Did ca. 10-15 sputtering-runs with minimal current (10mA) and finally put it in the scope at 5-6kV.
See images at http://www.electronmicroscopy.info/wax
It`s a bit of a crude solution but it works. Concerning the thickness of your wax layers you may also try the same thing with an high-vac chromecoater...
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
I'm not a biologist (most materials science) nor experimented in ESEM (only high vac SEM), but I've on my shelve a thermoelectric module (Pelletier/Seebeck device) which is 1.5x4 cm in dimensions.
Have a look at http://www.knap.at/datenblaetter/pel/pel_sup_kat-tem-e.pdf, where you can find a great variety of such devices. They give a Dt° in the 70°C range, and are avaible in several dimensions and power. From 10x10 to 62x62 are proposed, but rectanglaire or round shapes too. Of coarse, as an ESEM is under (bad) vacuum, thermal dissipation of the hot side is low. There is probably room enough to put a heat sink on the stage (copper block), but possibly one would need a water cooled one, what is probably not necessary on the device provided by FEI. If you have the possibilty to build something yourselve, it could be much cheaper. Of coarse without performences waranty !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
On 20/01/2011 13:02, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter. } } You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe. } } Does anyone know if this means the sample can be any size one likes? } } Dave } } -----Original Message----- } X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu] } Sent: 19 January 2011 22:53 } To: David Patton } Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu } Name: Robin Foley } } Organization: UAB } } Title-Subject: [Filtered] Environmental SEM for Biological Work } } Message: Is there heavy use of ESEM for biological SEM? What is } commonly done on biological ESEM samples? We're looking at } purchasing an environmental SEM and the sales rep. tells us we will } need a Peltier stage to cool the sample to look at wet samples. } Unfortunately, the maximum sample size for the Peltier stage is 3 mm } across. This seems tiny to me for an SEM. Are there many biological } ESEM applications that don't require the sample to be wet? } } Thanks, } } Robin Foley (who spends most of her SEM time looking at metals, } ceramics and polymers!) } } } } } Login Host: 138.26.80.193 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 22 -- From zaluzec-at-microscopy.com Wed Jan 19 16:50:11 2011 } 11, 22 -- Received: from znl.com ([206.69.208.20]) } 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JMoBNn026165 } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 } 11, 22 -- Received: from localhost (localhost [127.0.0.1]) } 11, 22 -- by znl.com (Postfix) with ESMTP id 89D5B316AFA } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 (CST) } 11, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 22 -- Received: from znl.com ([127.0.0.1]) } 11, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 22 -- with ESMTP id UF6QAiTpp+Ew for {microscopy-at-microscopy.com} ; 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Thu, 20 Jan 2011 11:52:54 +0000 (UTC) } 23, 48 -- Received: from mailapp03.uwe.ac.uk (164.11.132.65) by CH1EHSMHS030.bigfish.com } 23, 48 -- (10.43.70.30) with Microsoft SMTP Server id 14.1.225.8; Thu, 20 Jan 2011 } 23, 48 -- 11:52:53 +0000 } 23, 48 -- Received: from (egen-hub02.uwe.ac.uk [164.11.251.46]) by mailapp03.uwe.ac.uk } 23, 48 -- with smtp id 7bd0_2fac_cfd14bd6_248b_11e0_8ec4_00142221cca9; Thu, 20 Jan } 23, 48 -- 2011 11:52:52 +0000 } 23, 48 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 48 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.251.46]) with mapi; Thu, 20 Jan 2011 } 23, 48 -- 11:51:21 +0000 } 23, 48 -- From: David Patton {David.Patton-at-uwe.ac.uk} } 23, 48 -- To: "'rfoley-at-uab.edu'" {rfoley-at-uab.edu} } 23, 48 -- Date: Thu, 20 Jan 2011 11:51:20 +0000 } 23, 48 -- Subject: RE: [Microscopy] viaWWW: Environmental SEM for Biological Work } 23, 48 -- Thread-Topic: [Microscopy] viaWWW: Environmental SEM for Biological Work } 23, 48 -- Thread-Index: Acu4K66MlRtfppFTQyqwkR6SfbYJwwAa6AKw } 23, 48 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF433F9B96D72-at-EGEN-MBX02.campus.ads.uwe.ac.uk} } 23, 48 -- References: {201101192253.p0JMrH9m031418-at-ns.microscopy.com} } 23, 48 -- In-Reply-To: {201101192253.p0JMrH9m031418-at-ns.microscopy.com} } 23, 48 -- Accept-Language: en-US, en-GB } 23, 48 -- Content-Language: en-US } 23, 48 -- X-MS-Has-Attach: } 23, 48 -- X-MS-TNEF-Correlator: } 23, 48 -- acceptlanguage: en-US, en-GB } 23, 48 -- Content-Type: text/plain; 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==============================Original Headers============================== 8, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jan 21 09:29:38 2011 8, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LFTbmD020182 8, 30 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 09:29:37 -0600 8, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 8, 30 -- by mailhost.u-strasbg.fr (8.14.3/jtpda-5.5pre1) with ESMTP id p0LFTZcQ065634 8, 30 -- ; Fri, 21 Jan 2011 16:29:35 +0100 (CET) (envelope-from jacques.faerber-at-ipcms.u-strasbg.fr) 8, 30 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 8, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 30 -- (No client certificate requested) 8, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 8CB321000139; 8, 30 -- Fri, 21 Jan 2011 16:28:58 +0100 (CET) 8, 30 -- Message-ID: {4D39A656.9060606-at-ipcms.u-strasbg.fr} 8, 30 -- Date: Fri, 21 Jan 2011 16:29:26 +0100 8, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 8, 30 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.13) Gecko/20101208 Thunderbird/3.1.7 8, 30 -- MIME-Version: 1.0 8, 30 -- To: microscopy-at-microscopy.com, rfoley-at-uab.edu 8, 30 -- Subject: Re: Environmental SEM for Biological Work 8, 30 -- References: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- In-Reply-To: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-IPCMS-MailScanner: Found to be clean 8, 30 -- X-IPCMS-MailScanner-SpamScore: s 8, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 8, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.2.5 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 21 Jan 2011 16:29:35 +0100 (CET) 8, 30 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=T_RP_MATCHES_RCVD, 8, 30 -- USER_IN_WHITELIST autolearn=disabled version=3.3.1 8, 30 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr3.u-strasbg.fr ==============================End of - Headers==============================
Have you run spot analysis (boundary and middle of a grain) to confirm you can detect difference? From my experience with iron specimens, it is a pretty tough task to see boundary segregations even with WDS (at least when it is not a second phase, like carbides). X-from spot analysis you can estimate time needed for acquisition.
I do not know what element you are going to analyze, but if it is phosphorous, 20 kV is a way too high. For P you can use 4-5 kV and have a nice small zone of interaction.
Good luck
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} from: Frank_Karl-at-lincolnelectric.com } Date: 01/19/2011 05:45 AM } } I've been thinking about EDS line scans and I'm attempting to maximize } The data I collect. } } I run at 20 KV on iron samples so my electron interaction range is } between } 1.5 to 2.3um (Berthe or K-O range calculations). According to data } provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um } at } 700x. So right away I see if each image pixel represents a data point, } I'm } sampling about 2 pixel volumes per data point. Looking for a sudden } and } sharp change in element concentration seem difficult, but wait there's } more. Let us introduce another plot complication. } } My customers don't want to scan the entire image, tooo much data! } } So I scan a smaller section of image described above and instruct the } computer to take 512 data points along a line that might only be 400 } (visual estimate from screen) image pixels long. } } So... Should I reduce the image resolution so that each image pixel } will be } one data point and scan the entire image? Would it be better to lower } the } magnification so the image pixels are larger (say 1.2um ). I could go } to } 100x and 512 horizontal image pixel so each image pixel is 2.3um? } } I'm looking for slight changes in element concentration at grain } boundaries } and precipitates and of course I want to produce the most meaningful } data } possible. I'm running at conditions that suggest my electron } interactive } volume is larger than my spatial resolution as to produce "continuous" } data. } } Or am I just over thinking the process? } } Any suggestion or comments would be welcome } } Frank } Lincoln Electric Company } -- } ************************************************************* } Note: } The information contained in this message may be } privileged and confidential and protected from disclosure. If } the reader of this message is not the intended recipient, or } an employee or agent responsible for delivering this message } to the intended recipient, you are hereby notified that any } dissemination, distribution or copying of this communication } is strictly prohibited. If you have received this } communication in error, please notify us immediately by } replying to the message and deleting it from your computer. } Thank you, } The Lincoln Electric Company } **************************************************************
==============================Original Headers============================== 9, 32 -- From DusevichV-at-umkc.edu Fri Jan 21 10:44:52 2011 9, 32 -- Received: from mxnip01.um.umsystem.edu (mxnip01.um.umsystem.edu [209.106.229.21]) 9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LGiqXx009517 9, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 10:44:52 -0600 9, 32 -- X-IronPort-Anti-Spam-Filtered: true 9, 32 -- X-IronPort-Anti-Spam-Result: Ag4FAOtGOU3RauUp/2dsb2JhbACeegeFZHO0dIhohVAEhG+JWw 9, 32 -- Received: from um-nsmtpout1.um.umsystem.edu ([209.106.229.41]) 9, 32 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 21 Jan 2011 10:44:51 -0600 9, 32 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 9, 32 -- Fri, 21 Jan 2011 10:44:51 -0600 9, 32 -- Received: from um-email06.um.umsystem.edu ([169.254.1.184]) by 9, 32 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Fri, 21 Jan 2011 9, 32 -- 10:44:51 -0600 9, 32 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 9, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 32 -- Date: Fri, 21 Jan 2011 10:44:49 -0600 9, 32 -- Subject: RE: [Microscopy] EDS line scans and time on my hands 9, 32 -- Thread-Topic: [Microscopy] EDS line scans and time on my hands 9, 32 -- Thread-Index: Acu5G40UOmZxAGn/TK6djOViess4DAAa+xlQ 9, 32 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3A99B58CF92-at-UM-EMAIL06.um.umsystem.edu} 9, 32 -- References: {201101210330.p0L3UR8p002174-at-ns.microscopy.com} 9, 32 -- In-Reply-To: {201101210330.p0L3UR8p002174-at-ns.microscopy.com} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- acceptlanguage: en-US 9, 32 -- Content-Type: text/plain; charset="us-ascii" 9, 32 -- MIME-Version: 1.0 9, 32 -- X-OriginalArrivalTime: 21 Jan 2011 16:44:51.0181 (UTC) FILETIME=[85CFE1D0:01CBB98A] 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LGiqXx009517 ==============================End of - Headers==============================
I replied to Debby Sherman offline. In general stool specimen suspensions can be fixed in 2.5-5% paraformaldehyde prior to shipping. The specimens are then rendered noninfectious and can be maintained for an indefinite period at 4 degrees C. Multiple freezing and thawing, on occasion, can cause deleterious effects to viruses sufficient to render them difficult to identify by diagnostic negative stain electron microscopy.
Best regards,
Charles
Charles Humphrey US Centers for Disease Control and Prevention Mailstop G32 1600 Clifton Rd. Atlanta, GA 30333
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Wednesday, January 19, 2011 4:46 PM To: Humphrey, Charles (CDC/OID/NCEZID)
Hi all,
We will be imaging a fairly large number of norovirus samples using negative staining. This is just for sample screening to document the presence or absence of the virus.
The samples could be sent in fresh but I thought it might be better to fix them prior to shipping so that they are not a health hazard. That might also allow us to store them unfrozen for an extended period of time (months) with reduced chance of deterioration or contamination.
Does anyone have a validated protocol that they are willing to share? I used to know someone at the CDC who did TEM but no longer have that contact.
Thanks, Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 7, 28 -- From dsherman-at-purdue.edu Wed Jan 19 15:36:50 2011 7, 28 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JLanJM026947 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 15:36:50 -0600 7, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 7, 28 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0JLan3m008331 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:36:49 -0500 7, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.110]) by WPPEXHUB01F.purdue.lcl 7, 28 -- ([::1]) with mapi; Wed, 19 Jan 2011 16:36:48 -0500 7, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 7, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 28 -- Date: Wed, 19 Jan 2011 16:36:46 -0500 7, 28 -- Subject: Protocol for Norovirus TEM 7, 28 -- Thread-Topic: Protocol for Norovirus TEM 7, 28 -- Thread-Index: Acu4IPihFFWUNBgITEScuEiQwrSisg== 7, 28 -- Message-ID: {C95CC39E.B10D%dsherman-at-purdue.edu} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 7, 28 -- acceptlanguage: en-US 7, 28 -- Content-Type: text/plain; charset="us-ascii" 7, 28 -- MIME-Version: 1.0 7, 28 -- X-PMX-Version: 5.5.9.388399 7, 28 -- X-PerlMx-Virus-Scanned: Yes 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JLanJM026947 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 37 -- From cdh1-at-cdc.gov Fri Jan 21 11:51:58 2011 18, 37 -- Received: from mail165.messagelabs.com (mail165.messagelabs.com [216.82.253.147]) 18, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LHpw3e030635 18, 37 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 11:51:58 -0600 18, 37 -- X-VirusChecked: Checked 18, 37 -- X-Env-Sender: cdh1-at-cdc.gov 18, 37 -- X-Msg-Ref: server-9.tower-165.messagelabs.com!1295632310!40992952!7 18, 37 -- X-StarScan-Version: 6.2.9; banners=-,-,- 18, 37 -- X-Originating-IP: [158.111.190.156] 18, 37 -- Received: (qmail 16208 invoked from network); 21 Jan 2011 17:51:56 -0000 18, 37 -- Received: from unknown (HELO ECASHUB-CHAM3.cdc.gov) (158.111.190.156) 18, 37 -- by server-9.tower-165.messagelabs.com with AES128-SHA encrypted SMTP; 21 Jan 2011 17:51:56 -0000 18, 37 -- Received: from LTA3MF011.ees.hhs.gov (158.74.249.111) by ECASHUB-CHAM3.cdc.gov 18, 37 -- (158.111.190.156) with Microsoft SMTP Server id 14.0.702.0; Fri, 21 Jan 2011 18, 37 -- 12:51:04 -0500 18, 37 -- Received: from LTA3VS003.ees.hhs.gov ([158.74.248.102]) by 18, 37 -- LTA3MF011.ees.hhs.gov with Microsoft SMTPSVC(6.0.3790.3959); Fri, 21 Jan 18, 37 -- 2011 12:51:05 -0500 18, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 37 -- Content-Class: urn:content-classes:message 18, 37 -- MIME-Version: 1.0 18, 37 -- Content-Type: text/plain; charset="us-ascii" 18, 37 -- Subject: RE: [Microscopy] Protocol for Norovirus TEM 18, 37 -- Date: Fri, 21 Jan 2011 12:51:04 -0500 18, 37 -- Message-ID: {CBC4D3A10E913147808B5E5070217BD70F464E-at-LTA3VS003.ees.hhs.gov} 18, 37 -- In-Reply-To: {201101192145.p0JLjbrm014166-at-ns.microscopy.com} 18, 37 -- X-MS-Has-Attach: 18, 37 -- X-MS-TNEF-Correlator: 18, 37 -- Thread-Topic: [Microscopy] Protocol for Norovirus TEM 18, 37 -- Thread-Index: Acu4IjzCHHjx0vmfTWmz+2MTRdZvVgBbx0SA 18, 37 -- References: {201101192145.p0JLjbrm014166-at-ns.microscopy.com} 18, 37 -- From: "Humphrey, Charles (CDC/OID/NCEZID)" {cdh1-at-cdc.gov} 18, 37 -- To: {dsherman-at-purdue.edu} 18, 37 -- CC: {microscopy-at-microscopy.com} 18, 37 -- X-OriginalArrivalTime: 21 Jan 2011 17:51:05.0186 (UTC) FILETIME=[C680EC20:01CBB993] 18, 37 -- Content-Transfer-Encoding: 8bit 18, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LHpw3e030635 ==============================End of - Headers==============================
What is it about your tips that don't function? Are you sure it's not the sample or settings on the tool?
I spent some time last year doing STM with a DI3100 (/Veeco/Bruker) and made many many tips using the side cutter + pull method using Pt/Ir wire. I got some great images from HOPG samples to prove to myself that it was working properly.
The HOPG is highly recommended to get familiar with things (I got my sample from SPI, but Pella also has them). It will guarantee that your sample is not the problem, and allow low-mag imaging (over steps in layers) to very high mag imaging (lattice) to confirm that you have everything working properly.
What worked well for me was I would cut a tip slightly longer than I needed, then held one end with pliers while diagonal cutting/pulling away just the tip at the other end. The direction of cut started nearer the fixed end/pliers and ended up towards the free end. The part that was cut off (stuck to the cutters, flew across the table etc) was junk, the part left gripped in the pliers had a cut/stretched shape but was torn and jagged at the very end (under a microscope).
If you think about the way the sidecutters cut through the metal and plan on having the very end 'torn' you should be able to reliably get excellent tips. I taught the method to a couple of other people and they quickly managed to make similar tips without much trouble.
Chris Pawlowicz, B.Eng Manager, Lab Development UBM TechInsights 3000 Solandt Rd Ottawa ON Canada K2K 2X2 T: +1 613 576 0150 F: +1 613 599 6501 E: cpawlowicz-at-ubmtechinsights.com
I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf).
I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution.
The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses.
Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway.
Thanks,
Justin A. Kraft
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==============================Original Headers============================== 18, 25 -- From cpawlowicz-at-ubmtechinsights.com Fri Jan 21 12:09:20 2011 18, 25 -- Received: from pegasus.semiconductor.com (semi-fw.ubmtechinsights.com [207.61.67.10]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LI9I54015410 18, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 12:09:19 -0600 18, 25 -- Received: from pegasus.semiconductor.com ([192.168.2.10]) by pegasus 18, 25 -- ([192.168.2.10]) with mapi; Fri, 21 Jan 2011 13:09:14 -0500 18, 25 -- From: Chris Pawlowicz {cpawlowicz-at-ubmtechinsights.com} 18, 25 -- To: "'kraftpiano-at-gmail.com'" {kraftpiano-at-gmail.com} 18, 25 -- CC: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 18, 25 -- Date: Fri, 21 Jan 2011 13:09:13 -0500 18, 25 -- Subject: RE: [Microscopy] STM tip advice. 18, 25 -- Thread-Topic: [Microscopy] STM tip advice. 18, 25 -- Thread-Index: Acu5KiUJYDThOytvR2C07geBayGcHwAabGqw 18, 25 -- Message-ID: {0079587FD46DFC42AC07C9D337C7C895395FD0E387-at-pegasus} 18, 25 -- References: {201101210514.p0L5EufI017085-at-ns.microscopy.com} 18, 25 -- In-Reply-To: {201101210514.p0L5EufI017085-at-ns.microscopy.com} 18, 25 -- Accept-Language: en-US 18, 25 -- Content-Language: en-US 18, 25 -- X-MS-Has-Attach: 18, 25 -- X-MS-TNEF-Correlator: 18, 25 -- acceptlanguage: en-US 18, 25 -- Content-Type: text/plain; charset="us-ascii" 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Transfer-Encoding: 8bit 18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LI9I54015410 ==============================End of - Headers==============================
Something is always going on in VP and/or Environmental SEM: http://www.microscopy.org.au/ACMM20/VPSEMWorkshopOutline.pdf And, even a book: http://www.amazon.com/Principles-Practice-Variable-Pressure-Environmental/dp/0470065400
I have attended VP workshops at M&M meetings and I am reading the book.
Know it before you buy it. Our Quanta 400 (100mm stage) Mk1 has a water-cooled thermoelectric cold stage that will permit a specimen no larger than about 7mm. NOTE: PV=NRT is the key when one uses water vapor to pressurize the environment of an hydrated specimen.
Brendan Griffin has, I believe, done his original work on a XL-30, so he might have some suggestions as to what you might do in your deliberations.
My offerings of advice are as follows: 1. W or FEG - depends on requirements for resolution and data acquisition and analysis, because FEG is more expensive to maintain but absolutely necessary for high res imaging work alone. 2. Pressure ranges in VP SEM range from 1 Torr max to 20+ Torr max depending on platform, and therefore, the choice of platform will be based on range of expected uses to which the tool will be put and which gases one may wish to use. 3. Examples of uses we make of VP a. With our fully automated Oxford INCA EDS w/Feature/GSR, I have surveyed a 95 x 95 x 10mm slab of rock for grayscale thresholded ROI's or OOI's (2 sec acquisitions / object identified in a field) for OOI's so that the geologist could determine the area of the rock from which s/he would retrieve a thin section for further survey and probable selection for electron probe analysis (for dating). b. uncoated microfossils for imaging and retrieval. c. Backscatter image montages of large specimens (e.g. up to size in 1) d. There were rumors that at least on user imaged a specimen using H2SO4 vapor. e. Montage maps of large inclusions. 4. Before determining a choice, carefully create a list of expected and projected applications. a. The list defines the tool, if the list is very carefully assembled. 5. ALWAYS make know the total cost of ownership, including personnel, for a decade, and include in purchase as much annual service as possible. 6. Carefully spec the operating systems on the controlling computers with a sure knowledge of what will happen when the OS ceases to be supported, and user interface program will cease being supported by vendor. With our ESEM, the UI lost support before the OS (Win 2k Pro) did. a. My learned approach would be to assure management that THIS part of future support is clearly understood by both the supplier and the purchaser, with clear guarantees by the vendor in the purchase contract. That is, when/if there is a clear glitch in the Vendor's UI, there is a clear guarantee of support for a declared period of time. b. Recognize that each of your instruments may be the only one in the world to manifest a specific problem, and that the vendor's incentive is driven by numbers. c. Require a list of 3rd-party parts of the system you are purchasing. You will want to know how fare in the future your legacy bottlenecks might be. d. Recognize that in my world, at least, a control PC may cost a few to tens of thousands of dollars when replaced by the vendor when there is no contract. Ask what a stage replacement is at the moment, a control PC, a User Interface upgrade, and an estimate of what an upgrade of the disk operating system of the PC and the tool might be in 3-7 years (no vendor wants to give such an estimate, but remember that you have what they want. e. Recognize that you are likely to have any downtime reduced by turning service over to a 3rd party for an instrument under 10 years of age. Our TEM, after 8 years in service is quite similar in all respects to the model that is currently being marketed by FEI. FEI service on everything, and I strongly recommend it. 7. Make a set of the support documentation and parts lists a line item on your specification, and that if part numbers are changed in the future, you will be able to acquire them at no cost (for as long as you own the instrument). 7. The other matter is less obvious. Carefully spec your requirements for HV regulation and stability, in the context of your requirements for stability during an analysis as well as long-term reproducibility. This is difficult, but the HV system quite sophisticated and is repaired by total replacement rather than component replacement.
Finally, when our web site is back on line sometime next week, there are several papers related to VP SEM on the "Links" page that might be of interest to you and your prospective users.
Hope this helps,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: Friday, January 21, 2011 10:41 AM To: Monson, Frederick
Hi
I'm not a biologist (most materials science) nor experimented in ESEM (only high vac SEM), but I've on my shelve a thermoelectric module (Pelletier/Seebeck device) which is 1.5x4 cm in dimensions.
Have a look at http://www.knap.at/datenblaetter/pel/pel_sup_kat-tem-e.pdf, where you can find a great variety of such devices. They give a Dt° in the 70°C range, and are avaible in several dimensions and power. From 10x10 to 62x62 are proposed, but rectanglaire or round shapes too. Of coarse, as an ESEM is under (bad) vacuum, thermal dissipation of the hot side is low. There is probably room enough to put a heat sink on the stage (copper block), but possibly one would need a water cooled one, what is probably not necessary on the device provided by FEI. If you have the possibilty to build something yourselve, it could be much cheaper. Of coarse without performences waranty !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
On 20/01/2011 13:02, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter. } } You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe. } } Does anyone know if this means the sample can be any size one likes? } } Dave } } -----Original Message----- } X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu] } Sent: 19 January 2011 22:53 } To: David Patton } Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu } Name: Robin Foley } } Organization: UAB } } Title-Subject: [Filtered] Environmental SEM for Biological Work } } Message: Is there heavy use of ESEM for biological SEM? What is } commonly done on biological ESEM samples? We're looking at } purchasing an environmental SEM and the sales rep. tells us we will } need a Peltier stage to cool the sample to look at wet samples. } Unfortunately, the maximum sample size for the Peltier stage is 3 mm } across. This seems tiny to me for an SEM. Are there many biological } ESEM applications that don't require the sample to be wet? } } Thanks, } } Robin Foley (who spends most of her SEM time looking at metals, } ceramics and polymers!) } } } } } Login Host: 138.26.80.193 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 22 -- From zaluzec-at-microscopy.com Wed Jan 19 16:50:11 2011 } 11, 22 -- Received: from znl.com ([206.69.208.20]) } 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JMoBNn026165 } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 } 11, 22 -- Received: from localhost (localhost [127.0.0.1]) } 11, 22 -- by znl.com (Postfix) with ESMTP id 89D5B316AFA } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 (CST) } 11, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 22 -- Received: from znl.com ([127.0.0.1]) } 11, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 22 -- with ESMTP id UF6QAiTpp+Ew for {microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 8, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jan 21 09:29:38 2011 8, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LFTbmD020182 8, 30 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 09:29:37 -0600 8, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 8, 30 -- by mailhost.u-strasbg.fr (8.14.3/jtpda-5.5pre1) with ESMTP id p0LFTZcQ065634 8, 30 -- ; Fri, 21 Jan 2011 16:29:35 +0100 (CET) (envelope-from jacques.faerber-at-ipcms.u-strasbg.fr) 8, 30 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 8, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 30 -- (No client certificate requested) 8, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 8CB321000139; 8, 30 -- Fri, 21 Jan 2011 16:28:58 +0100 (CET) 8, 30 -- Message-ID: {4D39A656.9060606-at-ipcms.u-strasbg.fr} 8, 30 -- Date: Fri, 21 Jan 2011 16:29:26 +0100 8, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 8, 30 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.13) Gecko/20101208 Thunderbird/3.1.7 8, 30 -- MIME-Version: 1.0 8, 30 -- To: microscopy-at-microscopy.com, rfoley-at-uab.edu 8, 30 -- Subject: Re: Environmental SEM for Biological Work 8, 30 -- References: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- In-Reply-To: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-IPCMS-MailScanner: Found to be clean 8, 30 -- X-IPCMS-MailScanner-SpamScore: s 8, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 8, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.2.5 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 21 Jan 2011 16:29:35 +0100 (CET) 8, 30 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=T_RP_MATCHES_RCVD, 8, 30 -- USER_IN_WHITELIST autolearn=disabled version=3.3.1 8, 30 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr3.u-strasbg.fr ==============================End of - Headers==============================
==============================Original Headers============================== 25, 37 -- From FMonson-at-wcupa.edu Fri Jan 21 13:56:00 2011 25, 37 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 25, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LJtx5D007332 25, 37 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 13:55:59 -0600 25, 37 -- X-ASG-Debug-ID: 1295639758-0378c365de9f0a00001-4CH8be 25, 37 -- Received: from WCU-XCH-12.PASSHE.LCL (wcu-xch-12.wcupa.edu [144.26.0.96]) by MX02.WCUPA.EDU with ESMTP id oSATmgMVR7Md9em0; Fri, 21 Jan 2011 14:55:58 -0500 (EST) 25, 37 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 25, 37 -- X-Barracuda-Apparent-Source-IP: 144.26.0.96 25, 37 -- X-ASG-Whitelist: Client 25, 37 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 25, 37 -- WCU-XCH-12.PASSHE.LCL ([fe80::7112:a4e0:acfd:38d7%15]) with mapi id 25, 37 -- 14.01.0270.001; Fri, 21 Jan 2011 14:55:58 -0500 25, 37 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 25, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 25, 37 -- CC: "jacques.faerber-at-ipcms.u-strasbg.fr" {jacques.faerber-at-ipcms.u-strasbg.fr} , 25, 37 -- "rfoley-at-uab.edu" {rfoley-at-uab.edu} 25, 37 -- Subject: RE: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- Thread-Topic: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- Thread-Index: AQHLuYGjxFEkAm5al0SfU4vixxy7lJPbyuuw 25, 37 -- Date: Fri, 21 Jan 2011 19:55:57 +0000 25, 37 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C014C42-at-WCU-XCH-03.PASSHE.LCL} 25, 37 -- References: {201101211541.p0LFfEBr000930-at-ns.microscopy.com} 25, 37 -- In-Reply-To: {201101211541.p0LFfEBr000930-at-ns.microscopy.com} 25, 37 -- Accept-Language: en-US 25, 37 -- Content-Language: en-US 25, 37 -- X-MS-Has-Attach: 25, 37 -- X-MS-TNEF-Correlator: 25, 37 -- x-originating-ip: [10.28.57.79] 25, 37 -- Content-Type: text/plain; charset="iso-8859-1" 25, 37 -- MIME-Version: 1.0 25, 37 -- X-Barracuda-Connect: wcu-xch-12.wcupa.edu[144.26.0.96] 25, 37 -- X-Barracuda-Start-Time: 1295639758 25, 37 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 25, 37 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 25, 37 -- Content-Transfer-Encoding: 8bit 25, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LJtx5D007332 ==============================End of - Headers==============================
Hi Fred, Could you elaborate on item 6e? I'm a little confused.
I like some of your ideas to illuminate the probable life span of a system. Back when dinosaurs roamed the Earth, most people didn't pay much attention to life span because it was (usually correctly) assumed to be long.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: FMonson-at-wcupa.edu [mailto:FMonson-at-wcupa.edu] Sent: Friday, January 21, 2011 2:58 PM To: kenconverse-at-qualityimages.biz
Something is always going on in VP and/or Environmental SEM: http://www.microscopy.org.au/ACMM20/VPSEMWorkshopOutline.pdf And, even a book: http://www.amazon.com/Principles-Practice-Variable-Pressure-Environmental/dp /0470065400
I have attended VP workshops at M&M meetings and I am reading the book.
Know it before you buy it. Our Quanta 400 (100mm stage) Mk1 has a water-cooled thermoelectric cold stage that will permit a specimen no larger than about 7mm. NOTE: PV=NRT is the key when one uses water vapor to pressurize the environment of an hydrated specimen.
Brendan Griffin has, I believe, done his original work on a XL-30, so he might have some suggestions as to what you might do in your deliberations.
My offerings of advice are as follows: 1. W or FEG - depends on requirements for resolution and data acquisition and analysis, because FEG is more expensive to maintain but absolutely necessary for high res imaging work alone. 2. Pressure ranges in VP SEM range from 1 Torr max to 20+ Torr max depending on platform, and therefore, the choice of platform will be based on range of expected uses to which the tool will be put and which gases one may wish to use. 3. Examples of uses we make of VP a. With our fully automated Oxford INCA EDS w/Feature/GSR, I have surveyed a 95 x 95 x 10mm slab of rock for grayscale thresholded ROI's or OOI's (2 sec acquisitions / object identified in a field) for OOI's so that the geologist could determine the area of the rock from which s/he would retrieve a thin section for further survey and probable selection for electron probe analysis (for dating). b. uncoated microfossils for imaging and retrieval. c. Backscatter image montages of large specimens (e.g. up to size in 1) d. There were rumors that at least on user imaged a specimen using H2SO4 vapor. e. Montage maps of large inclusions. 4. Before determining a choice, carefully create a list of expected and projected applications. a. The list defines the tool, if the list is very carefully assembled. 5. ALWAYS make know the total cost of ownership, including personnel, for a decade, and include in purchase as much annual service as possible. 6. Carefully spec the operating systems on the controlling computers with a sure knowledge of what will happen when the OS ceases to be supported, and user interface program will cease being supported by vendor. With our ESEM, the UI lost support before the OS (Win 2k Pro) did. a. My learned approach would be to assure management that THIS part of future support is clearly understood by both the supplier and the purchaser, with clear guarantees by the vendor in the purchase contract. That is, when/if there is a clear glitch in the Vendor's UI, there is a clear guarantee of support for a declared period of time. b. Recognize that each of your instruments may be the only one in the world to manifest a specific problem, and that the vendor's incentive is driven by numbers. c. Require a list of 3rd-party parts of the system you are purchasing. You will want to know how fare in the future your legacy bottlenecks might be. d. Recognize that in my world, at least, a control PC may cost a few to tens of thousands of dollars when replaced by the vendor when there is no contract. Ask what a stage replacement is at the moment, a control PC, a User Interface upgrade, and an estimate of what an upgrade of the disk operating system of the PC and the tool might be in 3-7 years (no vendor wants to give such an estimate, but remember that you have what they want. e. Recognize that you are likely to have any downtime reduced by turning service over to a 3rd party for an instrument under 10 years of age. Our TEM, after 8 years in service is quite similar in all respects to the model that is currently being marketed by FEI. FEI service on everything, and I strongly recommend it. 7. Make a set of the support documentation and parts lists a line item on your specification, and that if part numbers are changed in the future, you will be able to acquire them at no cost (for as long as you own the instrument). 7. The other matter is less obvious. Carefully spec your requirements for HV regulation and stability, in the context of your requirements for stability during an analysis as well as long-term reproducibility. This is difficult, but the HV system quite sophisticated and is repaired by total replacement rather than component replacement.
Finally, when our web site is back on line sometime next week, there are several papers related to VP SEM on the "Links" page that might be of interest to you and your prospective users.
Hope this helps,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: Friday, January 21, 2011 10:41 AM To: Monson, Frederick
Hi
I'm not a biologist (most materials science) nor experimented in ESEM (only high vac SEM), but I've on my shelve a thermoelectric module (Pelletier/Seebeck device) which is 1.5x4 cm in dimensions.
Have a look at http://www.knap.at/datenblaetter/pel/pel_sup_kat-tem-e.pdf, where you can find a great variety of such devices. They give a Dt° in the 70°C range, and are avaible in several dimensions and power. From 10x10 to 62x62 are proposed, but rectanglaire or round shapes too. Of coarse, as an ESEM is under (bad) vacuum, thermal dissipation of the hot side is low. There is probably room enough to put a heat sink on the stage (copper block), but possibly one would need a water cooled one, what is probably not necessary on the device provided by FEI. If you have the possibilty to build something yourselve, it could be much cheaper. Of coarse without performences waranty !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
On 20/01/2011 13:02, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter. } } You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe. } } Does anyone know if this means the sample can be any size one likes? } } Dave } } -----Original Message----- } X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu] } Sent: 19 January 2011 22:53 } To: David Patton } Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu } Name: Robin Foley } } Organization: UAB } } Title-Subject: [Filtered] Environmental SEM for Biological Work } } Message: Is there heavy use of ESEM for biological SEM? What is } commonly done on biological ESEM samples? We're looking at } purchasing an environmental SEM and the sales rep. tells us we will } need a Peltier stage to cool the sample to look at wet samples. } Unfortunately, the maximum sample size for the Peltier stage is 3 mm } across. This seems tiny to me for an SEM. Are there many biological } ESEM applications that don't require the sample to be wet? } } Thanks, } } Robin Foley (who spends most of her SEM time looking at metals, } ceramics and polymers!) } } } } } Login Host: 138.26.80.193 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 22 -- From zaluzec-at-microscopy.com Wed Jan 19 16:50:11 2011 } 11, 22 -- Received: from znl.com ([206.69.208.20]) } 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JMoBNn026165 } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 } 11, 22 -- Received: from localhost (localhost [127.0.0.1]) } 11, 22 -- by znl.com (Postfix) with ESMTP id 89D5B316AFA } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 (CST) } 11, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 22 -- Received: from znl.com ([127.0.0.1]) } 11, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 22 -- with ESMTP id UF6QAiTpp+Ew for {microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 8, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jan 21 09:29:38 2011 8, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LFTbmD020182 8, 30 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 09:29:37 -0600 8, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 8, 30 -- by mailhost.u-strasbg.fr (8.14.3/jtpda-5.5pre1) with ESMTP id p0LFTZcQ065634 8, 30 -- ; Fri, 21 Jan 2011 16:29:35 +0100 (CET) (envelope-from jacques.faerber-at-ipcms.u-strasbg.fr) 8, 30 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 8, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 30 -- (No client certificate requested) 8, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 8CB321000139; 8, 30 -- Fri, 21 Jan 2011 16:28:58 +0100 (CET) 8, 30 -- Message-ID: {4D39A656.9060606-at-ipcms.u-strasbg.fr} 8, 30 -- Date: Fri, 21 Jan 2011 16:29:26 +0100 8, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 8, 30 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.13) Gecko/20101208 Thunderbird/3.1.7 8, 30 -- MIME-Version: 1.0 8, 30 -- To: microscopy-at-microscopy.com, rfoley-at-uab.edu 8, 30 -- Subject: Re: Environmental SEM for Biological Work 8, 30 -- References: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- In-Reply-To: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-IPCMS-MailScanner: Found to be clean 8, 30 -- X-IPCMS-MailScanner-SpamScore: s 8, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 8, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.2.5 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 21 Jan 2011 16:29:35 +0100 (CET) 8, 30 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=T_RP_MATCHES_RCVD, 8, 30 -- USER_IN_WHITELIST autolearn=disabled version=3.3.1 8, 30 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr3.u-strasbg.fr ==============================End of - Headers==============================
==============================Original Headers============================== 25, 37 -- From FMonson-at-wcupa.edu Fri Jan 21 13:56:00 2011 25, 37 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 25, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LJtx5D007332 25, 37 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 13:55:59 -0600 25, 37 -- X-ASG-Debug-ID: 1295639758-0378c365de9f0a00001-4CH8be 25, 37 -- Received: from WCU-XCH-12.PASSHE.LCL (wcu-xch-12.wcupa.edu [144.26.0.96]) by MX02.WCUPA.EDU with ESMTP id oSATmgMVR7Md9em0; Fri, 21 Jan 2011 14:55:58 -0500 (EST) 25, 37 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 25, 37 -- X-Barracuda-Apparent-Source-IP: 144.26.0.96 25, 37 -- X-ASG-Whitelist: Client 25, 37 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 25, 37 -- WCU-XCH-12.PASSHE.LCL ([fe80::7112:a4e0:acfd:38d7%15]) with mapi id 25, 37 -- 14.01.0270.001; Fri, 21 Jan 2011 14:55:58 -0500 25, 37 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 25, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 25, 37 -- CC: "jacques.faerber-at-ipcms.u-strasbg.fr" {jacques.faerber-at-ipcms.u-strasbg.fr} , 25, 37 -- "rfoley-at-uab.edu" {rfoley-at-uab.edu} 25, 37 -- Subject: RE: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- Thread-Topic: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- Thread-Index: AQHLuYGjxFEkAm5al0SfU4vixxy7lJPbyuuw 25, 37 -- Date: Fri, 21 Jan 2011 19:55:57 +0000 25, 37 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C014C42-at-WCU-XCH-03.PASSHE.LCL} 25, 37 -- References: {201101211541.p0LFfEBr000930-at-ns.microscopy.com} 25, 37 -- In-Reply-To: {201101211541.p0LFfEBr000930-at-ns.microscopy.com} 25, 37 -- Accept-Language: en-US 25, 37 -- Content-Language: en-US 25, 37 -- X-MS-Has-Attach: 25, 37 -- X-MS-TNEF-Correlator: 25, 37 -- x-originating-ip: [10.28.57.79] 25, 37 -- Content-Type: text/plain; charset="iso-8859-1" 25, 37 -- MIME-Version: 1.0 25, 37 -- X-Barracuda-Connect: wcu-xch-12.wcupa.edu[144.26.0.96] 25, 37 -- X-Barracuda-Start-Time: 1295639758 25, 37 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 25, 37 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 25, 37 -- Content-Transfer-Encoding: 8bit 25, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LJtx5D007332 ==============================End of - Headers==============================
==============================Original Headers============================== 38, 28 -- From kenconverse-at-qualityimages.biz Fri Jan 21 15:16:34 2011 38, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122]) 38, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LLGYDY024815 38, 28 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 15:16:34 -0600 38, 28 -- X-Authority-Analysis: v=1.1 cv=3jtQBdTzPyV+fq4oCU/u8ZPrJJGN11HvhaDVxyWhycI= c=1 sm=0 a=cCsHsa71OjYA:10 a=8nJEP1OIZ-IA:10 a=o/BqynnMLxMJiB4/byRFHg==:17 a=Zx37jsudAAAA:8 a=S-ikiy0eAAAA:8 a=vggBfdFIAAAA:8 a=8Wz4FX-BAAAA:8 a=SsN2on13AAAA:8 a=-gyhParZAAAA:8 a=pzmDz54eAAAA:8 a=jU2bWg6BAAAA:8 a=yMhMjlubAAAA:8 a=hCCmtxs2AAAA:8 a=F59vpjFj9DikHUfwRyUA:9 a=eUjghLitqcq2qX_Fj8IA:7 a=x3T5Eb86TmGMBu5YkpUYE0nMdL0A:4 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 a=fM1NKZWFy18A:10 a=7abxOcK5BwwA:10 a=BkF1guiUBFgA:10 a=RyjLG6jaqdoA:10 a=losLsr3N_5UA:10 a=k6cFIQMUckkA:10 a=3_2eAsHTqJoA:10 a=bsRvvyHUn3IA:10 a=G91thX30KR8A:10 a=UPymgFs0m_tAJC2f:21 a=gBBtCaMGKSruqQVW:21 a=o/BqynnMLxMJiB4/byRFHg==:117 38, 28 -- X-Cloudmark-Score: 0 38, 28 -- X-Originating-IP: 72.227.97.248 38, 28 -- Received: from [72.227.97.248] ([72.227.97.248:2040] helo=Ken) 38, 28 -- by cdptpa-oedge02.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 38, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 38, 28 -- id F9/7B-02631-1B7F93D4; Fri, 21 Jan 2011 21:16:34 +0000 38, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 38, 28 -- To: {FMonson-at-wcupa.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 38, 28 -- Subject: RE: [Microscopy] Environmental SEM for Biological Work 38, 28 -- Date: Fri, 21 Jan 2011 16:16:24 -0500 38, 28 -- Message-ID: {5CD9202EC2384D55BFE10F4932BF4D43-at-Ken} 38, 28 -- MIME-Version: 1.0 38, 28 -- Content-Type: text/plain; 38, 28 -- charset="iso-8859-1" 38, 28 -- X-Priority: 3 (Normal) 38, 28 -- X-MSMail-Priority: Normal 38, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 38, 28 -- Importance: Normal 38, 28 -- Thread-Index: Acu5pY5m+X84NQPuSFy3Y1W6iY1t2QACmmYg 38, 28 -- In-Reply-To: {201101211958.p0LJwKwM010950-at-ns.microscopy.com} 38, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 38, 28 -- Content-Transfer-Encoding: 8bit 38, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LLGYDY024815 ==============================End of - Headers==============================
Jun asked for a calibration grid with pitch near 0.1 um. My company makes and sells two relevant products: -a 70-nm pitch 1-dimensional pattern (lines and spaces) and -a 144-nm pitch square grid. These are available as ordinary calibration specimens or as standards traceable to the international meter. The traceable standards come with a test report that shows both the pitch value and its uncertainty. Please find further details at: http://www.asmicro.com/Supplies/utc.htm - traceable calibration specimens.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] 2009-2010 is our 20th year in business! =============================================
----- Original Message ----- From: junhe1970-at-gmail.com To: donc-at-asmicro.com Sent: Tuesday, December 21, 2010 8:05 AM Subject: [a] [Microscopy] viaWWW: SEM stardard grid
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Email: junhe1970-at-gmail.com Name: jun
Title-Subject: [Filtered] SEM stardard grid
Message: We frequently uses SEM ( Hitachi 4700, FE) to measure some wafer cross-section features. The measurement result is sometimes off the mark . I wonder if some one here could recommend some fine grid (0.1 um)standard Jun
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Organization: Wayne State Univ. School of Medicine
Title-Subject: [Filtered] Post-embedding/sectioning FluoroNanogold Labeling for TEM
Message: Hello, Some of our antigens are difficult to label in mammalian fat cells and muscles, such as particular lipid droplet proteins. I have tried several methods of antigen unmasking and probe maintenance in post-sectioning fluoronanogold labeling, including absence of uranyl acetate in the LR-White embedding medium, reduction of osmium post-fixation concentration to reduce etching propensity of silver-enhanced gold probes, antigen unmasking with oxidizing (sodium metaperiodate) and reducing (sodium borohydride) agents and Tris base pH 10. I have reduced my glutaraldehyde concentration to 0.10% and maintained paraformaldehyde at 2%.
Pre-embedding fluoronanogold labeling appears to work with the absence of glutaraldehyde in the fixative, however the ultrastructure is quite compromised, as one would expect by TEM.
I have been thinking now of using LR-Gold, until I hear from some experts on any other suggestions. Thank you very much! Vickie
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Email: j.knowles-at-ucl.ac.uk Name: Jonathan Knowles
Organization: UCL
Title-Subject: [Filtered] Electronics side of an EDX system
Message: Hi
We have inherited an EDX system which seems to work OK (sorry I don't have details to hand but wanted to ask this before I forgot!). The system works OK, but the electronics side is really ancient (It has a microVAX type drive with about 20Mb storage to run the OS and software) and am concerned that it might die some time. I wondered if anyone had ever built some more up to date hardware/interface? The connections for the EDX consist of a BNC for the HT and then a 9 pin connector presumably for data.
I am running a LEO1450VP SEM and encounter a problem related to the use of the mouse. The middle button of a 3-key mouse is used extremely in LEO32 software and suddenly the software disable the use of the middle button.
I am able to use both left and right button, except the middle one. I also tried to edit the toolbar to set the middle button’s function. However, both the “OK” and “Apply” buttons were deactivated so the settings I made were useless.
If anyone has similar experiences, please kindly contact me. Thank you very much.
Sean EMC -at- Saint Mary’s University Halifax, NS xyang-at-smu.ca
==============================Original Headers============================== 8, 23 -- From xyang-at-SMU.CA Mon Jan 24 13:12:57 2011 8, 23 -- Received: from HUSKY0.SMU.CA (Husky0.smu.ca [140.184.1.100]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0OJCuv1004741 8, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Jan 2011 13:12:57 -0600 8, 23 -- Received: from s422XY ("port 4882"-at-[140.184.128.189]) 8, 23 -- by HUSKY1.SMU.CA (PMDF V6.3-x13 #31544) 8, 23 -- with ESMTP id {01NX08EKYK4Y8XENI6-at-HUSKY1.SMU.CA} for 8, 23 -- Microscopy-at-microscopy.com; Mon, 24 Jan 2011 15:12:56 -0400 8, 23 -- Date: Mon, 24 Jan 2011 15:12:56 -0400 8, 23 -- From: Xiang Yang {xyang-at-SMU.CA} 8, 23 -- Subject: RE: middle mouse button problem in LEO SEM system 8, 23 -- In-reply-to: 8, 23 -- To: Microscopy-at-microscopy.com 8, 23 -- Reply-to: xyang-at-SMU.CA 8, 23 -- Message-id: {00a901cbbbfa$b4e166c0$1ea43440$-at-ca} 8, 23 -- MIME-version: 1.0 8, 23 -- X-Mailer: Microsoft Office Outlook 12.0 8, 23 -- Content-type: text/plain; charset=iso-8859-1 8, 23 -- Content-language: en-us 8, 23 -- Thread-Index: Acu7+lbpBvPk7hmmQoS04MHTTVYCrgAADeYA 8, 23 -- References: 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0OJCuv1004741 ==============================End of - Headers==============================
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Email: reynolds-at-vt.edu Name: Bill Reynolds
Organization: Virginia Tech
Title-Subject: [Filtered] Fwd: EDS line scans and time on my hands
Message: This comment does not directly address Frank Karl's original question about pixel size and measurement spacing, but it relates to the problem of detecting small changes in boundary concentrations.
Measuring grain boundary concentrations from EDS line scans is problematic because the electron beam geometry is always convoluted with the solute distribution in the sample. To determine, say, the maximum solute concentration at a grain boundary, you have to know the extent of beam broadening in the sample AND have an accurate model for how the solute is distributed across the boundary. If you don't already know where the solute is, you have to make an educated guess. For example, you might assume the solute is confined to a boundary region 1 nm thick so you can back-out a boundary concentration from the measured EDS profile, and the probe size with broadening. That involves a lot of assumptions and uncertainties.
A clever alternate strategy is to make an integrated measurement across a boundary to determine the total excess solute associated with the boundary (the excess solute is the amount of solute per unit area of grain boundary). This quantity is calculated from the difference between the amount of solute found in a sample volume that includes the boundary and the amount of solute found in an equivalent volume without the boundary (but located nearby).
The excess solute is not a concentration, and it tells you nothing about the solute distribution at a boundary, but it has some distinct advantages when trying to quantify solute enrichment or depletion at a boundary: it can be measured without knowing much about the probe size, beam broadening, or boundary structure, and you do not need to assume anything about the solute profile. Because the method employs a scanned box that straddles a boundary, it has better counting statistics than single line scans, and that makes it pretty good at detecting small solute enrichments.
The technique was developed with STEM in mind, but I don't see any reason why it cannot be used for EDS measurements in an SEM. Here are some example investigations that used the method: J.A.S. Ikeda, Y.-M. Chiang, and C.G. Madras: Ceram. Trans., 1991, vol. 24, pp. 341-348 A. J. Garratt-Reed: Proceedings of the 50th Annual Meeting, EMSA, 1992, p. 1206-1207 H.A. Fletcher, et al.: Scripta Mater., 2001, vol. 45 (5), pp. 561-567 E. S. Humphreys, et al. Metall. Mat. Trans A. vol 35A (4), 2004, pp 1223-1235.
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Email: sbarlow-at-sciences.sdsu.edu Name: Steve Barlow
Organization: EM Facility/San Diego State U Biology
Title-Subject: [Filtered] Uninterruptible power supply
Message: I am looking for a ups system for my Quanta 450 FESEM. If anyone has any experiences with various models, I would appreciate hearing about it.
A good source of knowledge for these thing is your IT department. Servers, especially those used in high power computing, tends to consume a lot of current and tend to fail badly when the power is interrupted.
cheers, logo
Gabriel Lapointe, M.Sc. gabriel.lapointe2-at-gmail.com http://gabriellapointe.ca
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On Mon, 2011-01-24 at 14:11 -0600, sbarlow-at-sciences.sdsu.edu wrote:
} Email: sbarlow-at-sciences.sdsu.edu } Name: Steve Barlow } } Organization: EM Facility/San Diego State U Biology } } Title-Subject: [Filtered] Uninterruptible power supply } } Message: I am looking for a ups system for my Quanta 450 FESEM. If } anyone has any experiences with various models, I would appreciate } hearing about it. } } The needed characteristics are: } Rating= 8 kVA/6.4 kW } Input voltage= 172-285 Vac } Input frequency= 40-70 Hz } Output voltage= 230 Vac } Output frequency= 50/60 Hz } } Thanks in advance. Vendors may contact me directly offline } } Steve } } Login Host: 146.244.234.42
==============================Original Headers============================== 16, 39 -- From gabriel.lapointe2-at-gmail.com Mon Jan 24 14:42:48 2011 16, 39 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 16, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0OKglYZ025568 16, 39 -- for {microscopy-at-microscopy.com} ; Mon, 24 Jan 2011 14:42:48 -0600 16, 39 -- Received: by vws10 with SMTP id 10so1943214vws.0 16, 39 -- for {microscopy-at-microscopy.com} ; Mon, 24 Jan 2011 12:42:47 -0800 (PST) 16, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 39 -- d=gmail.com; s=gamma; 16, 39 -- h=domainkey-signature:subject:from:to:in-reply-to:references 16, 39 -- :content-type:date:message-id:mime-version:x-mailer 16, 39 -- :content-transfer-encoding; 16, 39 -- bh=GVfBaQ+VCiRNLFcxJ0wPaQeKxFqy14XAt71oALhycsQ=; 16, 39 -- b=eRU8y3z70CsQ/rhz4BTxCxBnBZZ8lTDsIqi/oXUs6zF9n+kFyt4ep1wQ2ISrcW940d 16, 39 -- u1cE9IWZ8GDYp6SdwCDHmo1FM47aYx3heZkDaC3gkyRNDDtNNTh5dVN9V/5rQrabEPBF 16, 39 -- btm6oJe0eyYNeSaV/o4LYMd7KL5Sf6ywHmf8k= 16, 39 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 16, 39 -- d=gmail.com; s=gamma; 16, 39 -- h=subject:from:to:in-reply-to:references:content-type:date:message-id 16, 39 -- :mime-version:x-mailer:content-transfer-encoding; 16, 39 -- b=Bo0N/IclnOp4bEfaXhnspBRVdDeV17yeqoCn1mGlu7XT/+orl4wd0CdgpwyCtBvYeg 16, 39 -- mtTjg9ydLQfx7guPDrH/1EWJkHOagEeO/hT+u4XkrP2Lz/c6E657sWChFJU0JKKnbMe4 16, 39 -- BCazhlDq/lbSUQdNdybh5nMqDIqqZoVFdHlkw= 16, 39 -- Received: by 10.220.43.135 with SMTP id w7mr1259708vce.233.1295901765696; 16, 39 -- Mon, 24 Jan 2011 12:42:45 -0800 (PST) 16, 39 -- Received: from [132.204.85.122] (d-132-204-85-122.d-fac.umontreal.ca [132.204.85.122]) 16, 39 -- by mx.google.com with ESMTPS id p8sm4270212vcr.18.2011.01.24.12.42.44 16, 39 -- (version=SSLv3 cipher=RC4-MD5); 16, 39 -- Mon, 24 Jan 2011 12:42:45 -0800 (PST) 16, 39 -- Subject: Re: [Microscopy] viaWWW: Uninterruptible power supply 16, 39 -- From: Gabriel Lapointe {gabriel.lapointe2-at-gmail.com} 16, 39 -- To: microscopy-at-microscopy.com 16, 39 -- In-Reply-To: {201101242011.p0OKBX9c018428-at-ns.microscopy.com} 16, 39 -- References: {201101242011.p0OKBX9c018428-at-ns.microscopy.com} 16, 39 -- Content-Type: text/plain; charset="UTF-8" 16, 39 -- Date: Mon, 24 Jan 2011 15:42:42 -0500 16, 39 -- Message-ID: {1295901762.2679.10.camel-at-p0782954} 16, 39 -- Mime-Version: 1.0 16, 39 -- X-Mailer: Evolution 2.28.3 16, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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The insulator cable in our aged Edwards S150B sputter coater broke (so no HT) a few days ago. Does anyone have a clue on where can I order this part (or a compatible part from other makes) or get a service (I'm in Canada)?
We are recruiting a number of posts here in Oxford (UK), several of which might be of particular interest to readers here. If you know anyone who might be interested in these posts please do forward them this info. The closing dates are all Feb 3rd.
Micron Oxford is funded by a major grant from the Wellcome Trust to the Department of Biochemistry and the Sir William Dunn School of Pathology in association with the Wellcome Trust Centre for Human Genetics and The Oxford Center for Integrative Systems Biology. Its aim is to establish state of the art light and electron microscopy facilities located in the heart of the Oxford University research community with an emphasis on technology development.
We are currently trying to recruit an Assistant Facility Manager, a Correlative Light/Electron Microscopy Specialist and an Image Analysis Specialist. Full details can be found on our website http://www.micronoxford.com/
Additionally we are looking to appoint a Group Leader with expertise in the development or application of advanced imaging techniques or the modulation of protein function with a background in physics, chemistry or biology. We are also looking to appoint at senior post-doctoral level a system administrator specialising in the area of hierarchical data storage development/management. Full particulars of each post can be found at the Micron website (http://www.micronoxford.com/).
Thanks,
Ian -- Ian Dobbie Micron Imaging Facility Manager, Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU Tel: +44 (0)1865 613323 Email: ian.dobbie-at-bioch.ox.ac.uk
==============================Original Headers============================== 7, 22 -- From ian.dobbie-at-bioch.ox.ac.uk Tue Jan 25 07:26:31 2011 7, 22 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0PDQVUT021373 7, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Jan 2011 07:26:31 -0600 7, 22 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205]) 7, 22 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.71) 7, 22 -- (envelope-from {ian.dobbie-at-bioch.ox.ac.uk} ) 7, 22 -- id 1Phiuk-000891-VL 7, 22 -- for Microscopy-at-microscopy.com; Tue, 25 Jan 2011 13:26:30 +0000 7, 22 -- Received: from vpn6184.bioch.ox.ac.uk ([129.67.76.184] helo=oisins-macbook.home) 7, 22 -- by smtp2.mail.ox.ac.uk with esmtp (Exim 4.69) 7, 22 -- (envelope-from {ian.dobbie-at-bioch.ox.ac.uk} ) 7, 22 -- id 1Phiuk-00084f-7i 7, 22 -- for Microscopy-at-microscopy.com; Tue, 25 Jan 2011 13:26:30 +0000 7, 22 -- From: Ian Dobbie {ian.dobbie-at-bioch.ox.ac.uk} 7, 22 -- To: Microscopy-at-microscopy.com 7, 22 -- Subject: Recruiting correlative light/EM specialist, assistant facility manager and image analysis specialist. 7, 22 -- Date: Tue, 25 Jan 2011 13:25:28 +0000 7, 22 -- Message-ID: {g38vy96rnd.fsf-at-oisins-macbook.home} 7, 22 -- User-Agent: Gnus/5.110011 (No Gnus v0.11) Emacs/22.3 (darwin) 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
Organization: Wayne State Univ. School of Medicine
Title-Subject: [Filtered] Post-embedding/sectioning FluoroNanogold Labeling for TEM
Message: Hello, Some of our antigens are difficult to label in mammalian fat cells and muscles, such as particular lipid droplet proteins. I have tried several methods of antigen unmasking and probe maintenance in post-sectioning fluoronanogold labeling, including absence of uranyl acetate in the LR-White embedding medium, reduction of osmium post-fixation concentration to reduce etching propensity of silver-enhanced gold probes, antigen unmasking with oxidizing (sodium metaperiodate) and reducing (sodium borohydride) agents and Tris base pH 10. I have reduced my glutaraldehyde concentration to 0.10% and maintained paraformaldehyde at 2%.
Pre-embedding fluoronanogold labeling appears to work with the absence of glutaraldehyde in the fixative, however the ultrastructure is quite compromised, as one would expect by TEM.
I have been thinking now of using LR-Gold, until I hear from some experts on any other suggestions. Thank you very much! Vickie
Hi Vickie,
I had a similar situation and had successfully used pre-embed labelling with FluoroNanogold. I hope I can help. In the post-section fluoronanogold labelling, there might not be enough cross linking of your proteins to keep them stabilized especially during the dehydration and embedding steps. Antigen unmasking might not be the issue there but rather the proteins have been extracted. From what I recall lipids rely on osmium tetroxide and urany acetate fixation. Assuming you have abundant amount of these proteins, if you have already tried the gradual low temperature approach during dehydration and resin infiltration steps and there is still no labelling, I would try another approach.
You mentioned that pre-embed fluoronanogold labelling worked but the ultrastructure quality was quite compromised. That sounds promising when you get similar labelling pattern as in previous light microscopy data. In this case, you might be able to fine tune the fixation step and permeabilization if the latter was used. In my experience, this approach worked better for the Flower, a synaptic vesicle protein in the neuromuscular junction (NMJ) which we were working on. We tried several fixation combinations but found that overnight 4% paraformaldehyde fixation achieved sufficient labelling. Although ultrastructure quality was not the same as routine TEM, the organelles were still recognizable. We were a bit lucky because the NMJs lie close to the muscle surface and go only as deep as 5-8 microns into the tissue. Also, we worked on fillet-dissected third instar Drosophila larvae and did not need 50 micron thick vivatome sections. Even so, we still needed permeabilization steps but not as drastic as used in regular immunofluorescence. After trying different detergents and conc, we added 0.01% Tween 20 in the blocking step. The nice thing about using FluoroNanogold is we can examine the fluorescence labelling before proceeding to next steps. In addition, you can post fix with 3% Glut and use lower concentration of osmium tetroxide prior to dehydration and embedding so the sample quality should be more enhanced.
I am not an expert in Immunocytochemistry like Drs Paul Webster and Jan Leunissen and others out there but one thing I learned as I was getting into this is that there is no single labelling approach. Pre-embed method is not suitable for all samples but would likely work on cell monolayers/suspensions, vivatome sections and samples where antigen is easily accessible. We try to fit the best immuno-EM method to your samples which we all know usually takes time. I hope you have plenty of time to optimize the method for your samples.
I will send a pdf of the Flower paper so that you can check the micrographs. A detailed pre-embed method with Fluoronanogold is on the supplemental material.
Good luck,
Claire
------------------------------------------------------------ Claire M. Haueter Res. Tech. II (Electron Microscopy) Bellen Lab HHMI-Baylor College of Medicine Jan and Dan Duncan Neurological Research Institute 1250 Moursund St. Suite 1125 Mailstop NR-1125 Houston, TX 77030 Phone: 832-824-8772 Fax: 832-825-1240 Email: chaueter-at-bcm.edu -------------------------------------------------------------
==============================Original Headers============================== 2, 31 -- From chaueter-at-bcm.edu Tue Jan 25 11:20:03 2011 2, 31 -- Received: from iron1.corp.bcm.tmc.edu (iron1.corp.bcm.tmc.edu [128.249.224.13]) 2, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0PHK2ZE013593 2, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Jan 2011 11:20:03 -0600 2, 31 -- X-IronPort-AV: E=Sophos;i="4.60,375,1291615200"; 2, 31 -- d="scan'208";a="106861743" 2, 31 -- Received: from unknown (HELO EPW-EXHUB3-P022.ad.bcm.edu) ([10.22.132.49]) 2, 31 -- by iron1-out.bcm.edu with ESMTP; 25 Jan 2011 11:20:02 -0600 2, 31 -- Received: from EXCMSMBX03.ad.bcm.edu ([fe80::a5f2:b0d8:7a66:1bbf]) by 2, 31 -- EPW-EXHUB3-P022.ad.bcm.edu ([::1]) with mapi; Tue, 25 Jan 2011 11:20:02 -0600 2, 31 -- From: "Haueter, Claire Menoza" {chaueter-at-bcm.edu} 2, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 31 -- Date: Tue, 25 Jan 2011 11:20:02 -0600 2, 31 -- Subject: Reply on Post-embedding/sectioning FluoroNanogold labeling for TEM 2, 31 -- Thread-Topic: Reply on Post-embedding/sectioning FluoroNanogold labeling for 2, 31 -- TEM 2, 31 -- Thread-Index: AQHLvLQZqg//0Zu+HkardB4pWt5j2A== 2, 31 -- Message-ID: {F3EEF3767DFF3244826E72285C1C273448FA206741-at-EXCMSMBX03.ad.bcm.edu} 2, 31 -- Accept-Language: en-US 2, 31 -- Content-Language: en-US 2, 31 -- X-MS-Has-Attach: 2, 31 -- X-MS-TNEF-Correlator: 2, 31 -- acceptlanguage: en-US 2, 31 -- x-tm-as-product-ver: SMEX-8.0.0.1307-6.500.1024-17916.000 2, 31 -- x-tm-as-result: No--53.286300-8.000000-31 2, 31 -- x-tm-as-user-approved-sender: No 2, 31 -- x-tm-as-user-blocked-sender: No 2, 31 -- Content-Type: text/plain; charset="us-ascii" 2, 31 -- MIME-Version: 1.0 2, 31 -- Content-Transfer-Encoding: 8bit 2, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0PHK2ZE013593 ==============================End of - Headers==============================
The New York Structural Biology Center (NYSBC) seeks an experienced electron microscopist to join the staff of its Cryo-Electron Microscope Facility (http://cryoem.nysbc.org). The NYSBC is shared center that supports state-of-the-art research in cryo-EM, NMR, and X-ray. Cryo-EM facilities include four transmission electron microscopes and a new dual-beam scanning electron microscope, which support projects involving electron tomography, single particle analysis and electron crystallography of both stained and frozen-hydrated samples. In general, projects focus on 3D reconstruction of biological assemblies and examples range from the atomic structure of membrane proteins, to the subunit organization in macromolecular complexes and the cellular anatomy within the developing nematode. Implementation of new technologies is an ongoing interest at NYSBC and, with the dual-beam microscope, NYSBC plans to expand the scale of 3D reconstructions to encompass the characterization of entire cells and their distributions within their native tissue. To assist in these developments, NYSBC seeks a individual with postdoctoral experience in biological electron microscopy and image reconstruction. This individual will carry out experiments in support of collaborative projects with affiliated investigators, including EM sample preparation, data collection and image processing. Day-to-day duties also include assisting a wide range of users from the NYSBCąs ten Member Institutions and setup and maintenance of microscopes. The individual should be capable of multitasking and should enjoy working with other people. Good communication skills are essential. Qualified applicants should send a curriculum vitae and names of three references to David Stokes (stokes-at-nysbc.org). Salary is commensurate with experience. The position is currently open and applications will be reviewed continuously until the position is filled.
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==============================Original Headers============================== 5, 32 -- From Fengxia.Liang-at-med.nyu.edu Tue Jan 25 12:41:58 2011 5, 32 -- Received: from mail4.nyumc.org (mail4.nyumc.org [216.165.126.170]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0PIfwWC031891 5, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Jan 2011 12:41:58 -0600 5, 32 -- X-IronPort-Anti-Spam-Filtered: true 5, 32 -- X-IronPort-Anti-Spam-Result: ApsEAI+oPk0KkyXl/2dsb2JhbAClZbNmiGiFTwSFFw 5, 32 -- X-IronPort-AV: E=Sophos;i="4.60,375,1291611600"; 5, 32 -- d="scan'208";a="112814037" 5, 32 -- Received: from msgwsdcpht63.nyumc.org ([10.147.37.229]) 5, 32 -- by mail4.nyumc.org with SMTP; 25 Jan 2011 13:41:57 -0500 5, 32 -- Received: from MSGWSDCPMB05.nyumc.org ([10.147.54.52]) by 5, 32 -- MSGWSDCPHT63.nyumc.org ([10.147.37.229]) with mapi; Tue, 25 Jan 2011 13:41:57 5, 32 -- -0500 5, 32 -- From: "Liang, Fengxia" {Fengxia.Liang-at-med.nyu.edu} 5, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 32 -- Date: Tue, 25 Jan 2011 13:41:55 -0500 5, 32 -- Subject: Research Scientist Position in the Cryo-Electron Microscopy 5, 32 -- Facility at the New York Structural Biology Center 5, 32 -- Thread-Topic: Research Scientist Position in the Cryo-Electron Microscopy 5, 32 -- Facility at the New York Structural Biology Center 5, 32 -- Thread-Index: Acu8v4n8+leGz3MMmEyz3tESfhi+gg== 5, 32 -- Message-ID: {C96483A3.66F9%Fengxia.Liang-at-med.nyu.edu} 5, 32 -- Accept-Language: en-US 5, 32 -- Content-Language: en-US 5, 32 -- X-MS-Has-Attach: 5, 32 -- X-MS-TNEF-Correlator: 5, 32 -- user-agent: Microsoft-Entourage/13.4.0.100208 5, 32 -- acceptlanguage: en-US 5, 32 -- Content-Type: text/plain; charset="iso-8859-1" 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0PIfwWC031891 ==============================End of - Headers==============================
Microscopists, I am posting this on behalf of my colleague Mike Jercinovic. He is not a current subscriber so please be sure to cc him on any reply. His email is: mjj-at-geo.umass.edu. Thanks! TB
We have recently acquired an old Edwards Xenosput XE200 magnetron sputter coater. Does anyone have experience with these? It certainly produces an amazing coat, but has a rather bothersome problem. Often when we actuate the vacuum valve to start the initial pump down, or sometimes when we close the valve, the display goes haywire. The vacuum, head power, and head current readings all go crazy. The vacuum valve is pretty aggressive, so the whole thing gets a jolt when it opens or closes, so it seems like something might just be loose somewhere. This behavior can (sometimes) go away when switching the power off and on, or sometimes when cycling things a few times, but as this is happening quite frequently, the coater is almost unusable at this point. Today, it happened just when we vented it to put samples in. We got a "parts" machine along with the "working unit" (both obtained directly from Edwards), and I have now replaced the switching relay, the display electronics, the control electronics, and cables to the big board which has the terminal blocks....no change in behavior. I have reset all the physical interconnections I can find. Before launching in on further parts swapping, I thought it would be worth an enquiry to the community. Edwards no longer sells or supports this machine at all, so we are basically on our own.
Thanks for any help. Mike Jercinovic. UMass Geosciences.
==============================Original Headers============================== 3, 19 -- From baskin-at-bio.umass.edu Tue Jan 25 15:54:14 2011 3, 19 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0PLsEDI021802 3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 25 Jan 2011 15:54:14 -0600 3, 19 -- Received: from [172.30.52.170] (neutopia.bio.umass.edu [128.119.55.8]) 3, 19 -- (authenticated bits=0) 3, 19 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id p0PLsBaq001782 3, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 3, 19 -- Tue, 25 Jan 2011 16:54:12 -0500 (EST) 3, 19 -- Mime-Version: 1.0 3, 19 -- Message-Id: {p06240506c964f5e26fed-at-[172.30.52.170]} 3, 19 -- Date: Tue, 25 Jan 2011 16:54:10 -0500 3, 19 -- To: microscopy-at-microscopy.com 3, 19 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 3, 19 -- Subject: Edwards Xenosput XE200 problem 3, 19 -- Cc: Mike Jercinovic {mjj-at-geo.umass.edu} 3, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 19 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Tue, 25 Jan 2011 16:54:12 -0500 (EST) 3, 19 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
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Yes, the broken part is exactly as same as yours: at the bit on that connects to the head terminal. More frustrating, I can tell that it has broken at least 3 times from the record of usages--apparently a design flaw.
I might try to sold them together according to suggestions from the listers (I thank you all who replied to my email).
Best regards,
Guosheng Liu
----------------------- Hi there;
I was in the exact same boat as you, except I have an S150A coater. My HV cable broke (actually corroded and rotted away) right at the bit on that connects to the sputter head terminal. I called Edwards and spent a long time trying to even convince their service people that they made this piece of equipment. I ended up having to fax them copies of their own manual to show them the parts I was talking about.
Long story short: I never got anything from Edwards (useless). In the end I crimped to the end of the cable an eye-lug terminal and we just screw it onto the top of the sputter head now.
Best regards, Steven Cogswell, P.Eng. UNB Microscopy and Microanalysis
On Mon, Jan 24, 2011 at 8:15 PM, {gul417-at-mail.usask.ca} wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so } when replying } } please copy both gul417-at-mail.usask.ca as well as the } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: gul417-at-mail.usask.ca } } Name: Guosheng Liu } } } } Organization: University of Saskatchewan } } } } Title-Subject: [Filtered] Edwards S150B sputter coater part: } insulator/cable } } } } Message: Hello, } } } } The insulator cable in our aged Edwards S150B sputter coater broke } } (so no HT) a few days ago. Does anyone have a clue on where can I } } order this part (or a compatible part from other makes) or get a } } service (I'm in Canada)? } } } } Thanks in advance. } } } } Guosheng } }
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Artur.irani-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Artur.irani-at-yahoo.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] Beam problem
Message: Hi I am an EPMA SX100 Operator and it has a unknown problem during 2 weeks. when i choose HV(Kv) it getting on but HV is always became 0 , I checked the everywhere and changed Filament, also there is no error. but how much I try and give Hv it never changed. please help me in this situation. Kazem
This does not look like a self fix task as it seems that you have a problem with your high voltage system itself that is why it will not switch on. The only other possible reason is that the safety device which prevents the HT switching on under a poor vacuum could be at fault?
One question does the emission/beam current meter alter at all when you switch on the HT? If it does it is not the safety device.
Steve
-----Original Message----- X-from: Artur.irani-at-yahoo.com [mailto:Artur.irani-at-yahoo.com] Sent: 26 January 2011 13:57 To: protrain-at-emcourses.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Artur.irani-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Artur.irani-at-yahoo.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] Beam problem
Message: Hi I am an EPMA SX100 Operator and it has a unknown problem during 2 weeks. when i choose HV(Kv) it getting on but HV is always became 0 , I checked the everywhere and changed Filament, also there is no error. but how much I try and give Hv it never changed. please help me in this situation. Kazem
For those interested in cellular microscopy there is now The Cell: An Image Library - http://www.cellimagelibrary.org/
Also feel free to join The Cell LinkedIn Group - http://www.linkedin.com/groupRegistration?gid=3733425
David
David Orloff Manager, Image Library The American Society for Cell Biology 8120 Woodmont Avenue, Suite 750 Bethesda, MD 20814-2762 T: 301-347-9300/Direct: 301-347-9305 F: 301-347-9310 E-mail: dorloff-at-ascb.org Web site: www.ascb.org The Cell: An Image LibraryT: www.cellimagelibrary.org LinkedIn Group: http://www.linkedin.com/groupRegistration?gid=3733425
==============================Original Headers============================== 8, 23 -- From DOrloff-at-ascb.org Wed Jan 26 09:12:03 2011 8, 23 -- Received: from smtp.ascb.org (jscpp.org [173.13.233.102] (may be forged)) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0QFC3I3017865 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Jan 2011 09:12:03 -0600 8, 23 -- Received: from ASCB2.ascb.local ([::1]) by ASCB2.ascb.local ([::1]) with mapi; 8, 23 -- Wed, 26 Jan 2011 10:12:00 -0500 8, 23 -- From: David Orloff {DOrloff-at-ascb.org} 8, 23 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 8, 23 -- CC: David Orloff {DOrloff-at-ascb.org} 8, 23 -- Date: Wed, 26 Jan 2011 10:11:59 -0500 8, 23 -- Subject: The Cell: An Image Library - LinkedIn group 8, 23 -- Thread-Topic: The Cell: An Image Library - LinkedIn group 8, 23 -- Thread-Index: Acu9a2D302NV3t/tQGqajHGfrnk6xQ== 8, 23 -- Message-ID: {8D9C2A7731877D45B1691E78451EC3BE4C6BE127-at-ASCB2.ascb.local} 8, 23 -- Accept-Language: en-US 8, 23 -- Content-Language: en-US 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- acceptlanguage: en-US 8, 23 -- Content-Type: text/plain; charset="iso-8859-1" 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0QFC3I3017865 ==============================End of - Headers==============================
Hello, We have another problem with our Philips CM100. Yesterday afternoon, the operating system of the microscope crashed with strange OPCON screen (see the link bellow for image). No beeps, no buttons were working with exception of the "OFF button". After restart, CM100 started in “Default RAM init” mode.
Please, did anybody observed something similar? In our case it was for the first time. Any responses are welcome.
Image of OPCON screen after crash: http://www2.biomed.cas.cz/~benada/CM100_trouble.html
Best regards Oldrich
-------------------------- Oldrich Benada Institute of Microbiology, Acad. Sci. CR Videnska 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 6, 21 -- From benada-at-biomed.cas.cz Thu Jan 27 04:37:17 2011 6, 21 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0RAbGmx020693 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 27 Jan 2011 04:37:17 -0600 6, 21 -- Received: from u117ob.localnet (a100ix.mbu.cas.cz [147.231.44.171]) 6, 21 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 21 -- (No client certificate requested) 6, 21 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id C851C1A44726 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 27 Jan 2011 11:37:15 +0100 (CET) 6, 21 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 6, 21 -- Organization: =?utf-8?q?Mikrobiologck=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?= 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- Subject: CM100 trouble 6, 21 -- Date: Thu, 27 Jan 2011 11:37:09 +0100 6, 21 -- User-Agent: KMail/1.13.5 (Linux/2.6.32-5-686; KDE/4.4.5; i686; ; ) 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; 6, 21 -- charset="utf-8" 6, 21 -- Message-Id: {201101271137.09396.benada-at-biomed.cas.cz} 6, 21 -- Content-Transfer-Encoding: 8bit 6, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0RAbGmx020693 ==============================End of - Headers==============================
How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year old NMR machine? If both are on the same floor, then the magnetic field lines from the NMR should run through the TEM column vertically, so that the sensitivity for the strong DC fields of the NMR machine might be less ?
I would appreciate any experience or recommendations, also directly towards me.
Henning Stahlberg, PhD. Center for Cellular Imaging and Nano Analytics (C-CINA) Prof. for Structural Biology Biozentrum, University Basel at the Department of Biosystems Science and Engineering (D-BSSE) Mattenstrasse 26, WRO-1058 CH-4058 Basel, Switzerland Tel: +41-61-387 32 62 (office) Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant) Fax: +41-61-387 39 86 mailto:Henning.Stahlberg-at-unibas.ch Skype:henningstahlberg http://c-cina.org http://2dx.org
Postal Address for delivery of Letters / Goods / Express Carriers: C-CINA, Biozentrum University of Basel c/o Syngenta AG, WRO-1058.6.60, D-BSSE Schwarzwaldallee 215 Postfach CH-4002 Basel ___________________________________________________
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Email: wxufocus-at-gmail.com Name: Jizhong
Title-Subject: [Filtered] LM: Navitar Tube Lens for use with Infinity Corrected Objective
Message: Hi, I'm setting up an optical system with an infinity corrected objective lens in a special tool I'm designing. I'm thinking of using an adapter tube from Navitar to form the image for a CCD camera. The Adapter Tube has a nice C-mount for attaching the CCD camera. My question is: is the tube lens (to convert the parallel beams into a real image) resides inside the Adapter Tube? Or I need the Ultra Body Tube? Please see the sys diagram: http://machinevision.navitar.com/pdfs/Precise_Eye_System_Diagram.pdf
I am no longer the Director of Light Microscopy at St. Jude Children's Research Hospital. For questions regarding the use of the Cell and Tissue Imaging Center, please direct your inquiries to the team of Imaging Scientists in the CTIC, Jennifer Peters, Yannan Ouyang, and Jamshid Temirov. They may be reached as a group at the following email address: lightmicroscopy-at-stjude.org. The phone number in the CTIC is 901-595-3439.
Although no longer serving in my former capacity as the Director of Light Microscopy, I am currently available as a technical consultant to the Cell and Tissue Imaging Center. I may be reached directly as necessary at the following email address: samuel.connell-at-gmail.com
I am currently out of the office on extended leave. For assistance, please contact Mary Arnold (Mary.Arnold-at-MyFWC.com) and she will direct your inquiry to the appropriate staff member.
Earnest Truby
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Hello, Many thanks for all advices and suggestions. The most likely it was a power spike that caused the problem. Now, our CM100 is working again. Unfortunately, we have only DOS remote control package for microscope setting backup and our old DOS PC does not boot any more. It took me half a day to align and recalibrate the microscope manually after its RAM init. I'm thinking about setup a virtual box with DOS and remote control package in near future.
I merged together all the replies and they are available on following link: http://www2.biomed.cas.cz/~benada/CM100_trouble_r.html
Many thanks again Oldrich
} } Hello, } } We have another problem with our Philips CM100. Yesterday afternoon, the } } operating system of the microscope crashed with strange OPCON screen } } (see the link bellow for image). No beeps, no buttons were working with } } exception of the "OFF button". After restart, CM100 started in “Default } } RAM init” mode. } } } } Please, did anybody observed something similar? In our case it was for } } the first time. Any responses are welcome. } } } } Image of OPCON screen after crash: } } http://www2.biomed.cas.cz/~benada/CM100_trouble.html } } } } Best regards } } } } Oldrich } } } } -------------------------- } } Oldrich Benada } } Institute of Microbiology, Acad. Sci. CR } } Videnska 1083 } } 142 20 Prague 4 } } Czech Republic
==============================Original Headers============================== 5, 23 -- From benada-at-biomed.cas.cz Fri Jan 28 07:27:17 2011 5, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SDRHtI015581 5, 23 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 07:27:17 -0600 5, 23 -- Received: from u117ob.localnet (u117ob.mbu.cas.cz [147.231.44.101]) 5, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 23 -- (No client certificate requested) 5, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 6439D1A44664 5, 23 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 14:27:16 +0100 (CET) 5, 23 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 5, 23 -- Organization: =?utf-8?q?Mikrobiologck=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?= 5, 23 -- To: microscopy-at-microscopy.com 5, 23 -- Subject: Re: [Microscopy] CM100 trouble - solved (hopefully) 5, 23 -- Date: Fri, 28 Jan 2011 14:27:08 +0100 5, 23 -- User-Agent: KMail/1.13.5 (Linux/2.6.32-5-686; KDE/4.4.5; i686; ; ) 5, 23 -- References: {201101271037.p0RAbUuW020819-at-ns.microscopy.com} {C0F02D25-2F23-4835-9929-5C16610E5E47-at-rochester.rr.com} 5, 23 -- In-Reply-To: {C0F02D25-2F23-4835-9929-5C16610E5E47-at-rochester.rr.com} 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: Text/Plain; 5, 23 -- charset="windows-1252" 5, 23 -- Message-Id: {201101281427.09048.benada-at-biomed.cas.cz} 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0SDRHtI015581 ==============================End of - Headers==============================
I'm not sure of the distance, but you should be able to use a cheap gaussmeter to measure the fields from the NMR. Even a DC field can cause problems for a TEM, particularly a high-end FEG TEM. Anyone who has worked with magnetic samples can attest to the problems they cause.
While there may be strong DC fields, NMRs work by flipping nuclear magnetic moments which suggests there are AC or pulsed components to the field.
You do not want this beast close to your TEM column.
Cheers, Henk
At 1/28/2011 5:37 AM, hstahlberg-at-me.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Hi, } } How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year old NMR machine? If both are on the same floor, then the magnetic field lines from the NMR should run through the TEM column vertically, so that the sensitivity for the strong DC fields of the NMR machine might be less ? } } I would appreciate any experience or recommendations, also directly towards me. } } Thanks, } } Henning. } } ___________________________________________________ } } Henning Stahlberg, PhD. } Center for Cellular Imaging and Nano Analytics (C-CINA) } Prof. for Structural Biology } Biozentrum, University Basel } at the Department of Biosystems Science and Engineering (D-BSSE) } Mattenstrasse 26, WRO-1058 } CH-4058 Basel, Switzerland } Tel: +41-61-387 32 62 (office) } Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant) } Fax: +41-61-387 39 86 } mailto:Henning.Stahlberg-at-unibas.ch } Skype:henningstahlberg } http://c-cina.org } http://2dx.org } } Postal Address for delivery of Letters / Goods / Express Carriers: } C-CINA, Biozentrum University of Basel } c/o Syngenta AG, WRO-1058.6.60, D-BSSE } Schwarzwaldallee 215 } Postfach } CH-4002 Basel } ___________________________________________________ } } ==============================Original Headers============================== } 10, 23 -- Fromhstahlberg-at-me.com Fri Jan 28 04:36:31 2011 } 10, 23 -- Received: from asmtpout020.mac.com (asmtpout020.mac.com [17.148.16.95]) } 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SAaVKt022583 } 10, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 04:36:31 -0600 } 10, 23 -- MIME-version: 1.0 } 10, 23 -- Content-transfer-encoding: 7BIT } 10, 23 -- Content-type: text/plain; CHARSET=US-ASCII } 10, 23 -- Received: from [192.168.1.35] ([92.104.21.174]) } 10, 23 -- by asmtp020.mac.com (Oracle Communications Messaging Exchange Server 7u4-20.01 } 10, 23 -- 64bit (built Nov 21 2010)) with ESMTPSA id {0LFQ009UNARUIS90-at-asmtp020.mac.com} } 10, 23 -- forMicroscopy-at-microscopy.com; Fri, 28 Jan 2011 02:36:31 -0800 (PST) } 10, 23 -- X-Proofpoint-Virus-Version: vendor=fsecure } 10, 23 -- engine=2.50.10432:5.2.15,1.0.148,0.0.0000 } 10, 23 -- definitions=2011-01-28_04:2011-01-28,2011-01-28,1970-01-01 signatures=0 } 10, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 } 10, 23 -- ipscore=0 suspectscore=1 phishscore=0 bulkscore=0 adultscore=0 classifier=spam } 10, 23 -- adjust=0 reason=mlx engine=6.0.2-1012030000 definitions=main-1101280028 } 10, 23 -- From: Henning Stahlberg {hstahlberg-at-me.com} } 10, 23 -- Subject: How close can a NMR machine be installed next to a TEM } 10, 23 -- Date: Fri, 28 Jan 2011 11:35:53 +0100 } 10, 23 -- Message-id: {A81EC18B-D20C-4687-9FEA-E77A009400F7-at-me.com} } 10, 23 -- To:Microscopy-at-microscopy.com } 10, 23 -- X-Mailer: Apple Mail (2.1082) } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 8, 26 -- From colijn.1-at-osu.edu Fri Jan 28 07:46:42 2011 8, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SDkgWe015146 8, 26 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 07:46:42 -0600 8, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 8, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 26 -- id {01NX5G33RU409EGJJ4-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 8, 26 -- Fri, 28 Jan 2011 08:46:42 -0500 (EST) 8, 26 -- Received: from [164.107.76.202] (hoc3.ceof.ohio-state.edu [164.107.76.202]) 8, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 26 -- with ESMTPA id {01NX5G33FUQS9EIROA-at-ecr6.ohio-state.edu} for 8, 26 -- microscopy-at-microscopy.com; Fri, 28 Jan 2011 08:46:42 -0500 (EST) 8, 26 -- Date: Fri, 28 Jan 2011 08:46:41 -0500 8, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 8, 26 -- Subject: Re: [Microscopy] How close can a NMR machine be installed next to a TEM 8, 26 -- In-reply-to: {201101281037.p0SAbvsr024540-at-ns.microscopy.com} 8, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 8, 26 -- To: microscopy-at-microscopy.com 8, 26 -- Message-id: {4D42C8C1.3090601-at-osu.edu} 8, 26 -- MIME-version: 1.0 8, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 26 -- Content-transfer-encoding: 7bit 8, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) 8, 26 -- Gecko/20101207 Thunderbird/3.1.7 8, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 8, 26 -- References: {201101281037.p0SAbvsr024540-at-ns.microscopy.com} ==============================End of - Headers==============================
We have a Philips CM10 TEM. Jeol checked for problems prior to installing a Jeol Eclipse 300MHz superconducting magnet instrument 13m and two rooms away. As far as we know there was no interference.
Dave
-----Original Message----- X-from: hstahlberg-at-me.com [mailto:hstahlberg-at-me.com] Sent: 28 January 2011 10:40 To: David Patton
Hi,
How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year old NMR machine? If both are on the same floor, then the magnetic field lines from the NMR should run through the TEM column vertically, so that the sensitivity for the strong DC fields of the NMR machine might be less ?
I would appreciate any experience or recommendations, also directly towards me.
Henning Stahlberg, PhD. Center for Cellular Imaging and Nano Analytics (C-CINA) Prof. for Structural Biology Biozentrum, University Basel at the Department of Biosystems Science and Engineering (D-BSSE) Mattenstrasse 26, WRO-1058 CH-4058 Basel, Switzerland Tel: +41-61-387 32 62 (office) Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant) Fax: +41-61-387 39 86 mailto:Henning.Stahlberg-at-unibas.ch Skype:henningstahlberg http://c-cina.org http://2dx.org
Postal Address for delivery of Letters / Goods / Express Carriers: C-CINA, Biozentrum University of Basel c/o Syngenta AG, WRO-1058.6.60, D-BSSE Schwarzwaldallee 215 Postfach CH-4002 Basel ___________________________________________________
==============================Original Headers============================== 10, 23 -- From hstahlberg-at-me.com Fri Jan 28 04:36:31 2011 10, 23 -- Received: from asmtpout020.mac.com (asmtpout020.mac.com [17.148.16.95]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SAaVKt022583 10, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 04:36:31 -0600 10, 23 -- MIME-version: 1.0 10, 23 -- Content-transfer-encoding: 7BIT 10, 23 -- Content-type: text/plain; CHARSET=US-ASCII 10, 23 -- Received: from [192.168.1.35] ([92.104.21.174]) 10, 23 -- by asmtp020.mac.com (Oracle Communications Messaging Exchange Server 7u4-20.01 10, 23 -- 64bit (built Nov 21 2010)) with ESMTPSA id {0LFQ009UNARUIS90-at-asmtp020.mac.com} 10, 23 -- for Microscopy-at-microscopy.com; Fri, 28 Jan 2011 02:36:31 -0800 (PST) 10, 23 -- X-Proofpoint-Virus-Version: vendor=fsecure 10, 23 -- engine=2.50.10432:5.2.15,1.0.148,0.0.0000 10, 23 -- definitions=2011-01-28_04:2011-01-28,2011-01-28,1970-01-01 signatures=0 10, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 10, 23 -- ipscore=0 suspectscore=1 phishscore=0 bulkscore=0 adultscore=0 classifier=spam 10, 23 -- adjust=0 reason=mlx engine=6.0.2-1012030000 definitions=main-1101280028 10, 23 -- From: Henning Stahlberg {hstahlberg-at-me.com} 10, 23 -- Subject: How close can a NMR machine be installed next to a TEM 10, 23 -- Date: Fri, 28 Jan 2011 11:35:53 +0100 10, 23 -- Message-id: {A81EC18B-D20C-4687-9FEA-E77A009400F7-at-me.com} 10, 23 -- To: Microscopy-at-microscopy.com 10, 23 -- X-Mailer: Apple Mail (2.1082) ==============================End of - Headers==============================
==============================Original Headers============================== 20, 49 -- From David.Patton-at-uwe.ac.uk Fri Jan 28 09:26:18 2011 20, 49 -- Received: from DB3EHSOBE005.bigfish.com (db3ehsobe005.messaging.microsoft.com [213.199.154.143]) 20, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SFQH8A007281 20, 49 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 09:26:17 -0600 20, 49 -- Received: from mail57-db3-R.bigfish.com (10.3.81.247) by 20, 49 -- DB3EHSOBE005.bigfish.com (10.3.84.25) with Microsoft SMTP Server id 20, 49 -- 14.1.225.8; Fri, 28 Jan 2011 15:26:16 +0000 20, 49 -- Received: from mail57-db3 (localhost.localdomain [127.0.0.1]) by 20, 49 -- mail57-db3-R.bigfish.com (Postfix) with ESMTP id 741F8AD8278; Fri, 28 Jan 20, 49 -- 2011 15:26:16 +0000 (UTC) 20, 49 -- X-SpamScore: -25 20, 49 -- X-BigFish: VPS-25(zzbb2cK936eK7f0R542N4015Lzz1202hz31iz8275bh8275dhz2fh668h) 20, 49 -- X-Forefront-Antispam-Report: KIP:(null);UIP:(null);IPVD:NLI;H:mailapp04.uwe.ac.uk;RD:mailapp04.uwe.ac.uk;EFVD:NLI 20, 49 -- Received: from mail57-db3 (localhost.localdomain [127.0.0.1]) by mail57-db3 20, 49 -- (MessageSwitch) id 1296228376153955_25127; Fri, 28 Jan 2011 15:26:16 +0000 20, 49 -- (UTC) 20, 49 -- Received: from DB3EHSMHS011.bigfish.com (unknown [10.3.81.254]) by 20, 49 -- mail57-db3.bigfish.com (Postfix) with ESMTP id 215BF128804C; Fri, 28 Jan 2011 20, 49 -- 15:26:16 +0000 (UTC) 20, 49 -- Received: from mailapp04.uwe.ac.uk (164.11.132.66) by DB3EHSMHS011.bigfish.com 20, 49 -- (10.3.87.111) with Microsoft SMTP Server id 14.1.225.8; Fri, 28 Jan 2011 20, 49 -- 15:26:12 +0000 20, 49 -- Received: from (egen-hub01.uwe.ac.uk [164.11.251.45]) by mailapp04.uwe.ac.uk 20, 49 -- with smtp id 784e_1182_f038fb40_2af2_11e0_b166_00142223915c; Fri, 28 Jan 20, 49 -- 2011 15:26:12 +0000 20, 49 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by 20, 49 -- egen-hub01.campus.ads.uwe.ac.uk ([164.11.251.45]) with mapi; Fri, 28 Jan 2011 20, 49 -- 15:24:56 +0000 20, 49 -- From: David Patton {David.Patton-at-uwe.ac.uk} 20, 49 -- To: "'hstahlberg-at-me.com'" {hstahlberg-at-me.com} 20, 49 -- Date: Fri, 28 Jan 2011 15:24:57 +0000 20, 49 -- Subject: RE: [Microscopy] How close can a NMR machine be installed next to a 20, 49 -- TEM 20, 49 -- Thread-Topic: [Microscopy] How close can a NMR machine be installed next to 20, 49 -- a TEM 20, 49 -- Thread-Index: Acu+17j4uzXKSG+KT0+0K5ae79FPDAAJ0GMA 20, 49 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF433F9B96DCB-at-EGEN-MBX02.campus.ads.uwe.ac.uk} 20, 49 -- References: {201101281040.p0SAe1l6027719-at-ns.microscopy.com} 20, 49 -- In-Reply-To: {201101281040.p0SAe1l6027719-at-ns.microscopy.com} 20, 49 -- Accept-Language: en-US, en-GB 20, 49 -- Content-Language: en-US 20, 49 -- X-MS-Has-Attach: 20, 49 -- X-MS-TNEF-Correlator: 20, 49 -- acceptlanguage: en-US, en-GB 20, 49 -- Content-Type: text/plain; charset="us-ascii" 20, 49 -- MIME-Version: 1.0 20, 49 -- X-OriginatorOrg: uwe.ac.uk 20, 49 -- Content-Transfer-Encoding: 8bit 20, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0SFQH8A007281 ==============================End of - Headers==============================
Part of our microscopy lab is next door to the NMR lab. The high electro-magnetic fields (presumably from the high voltage lines feeding the NMR) preclude installation of SEM or TEM in areas near the adjoining wall. A distance of only a few meters is sufficient for the field strength to drop to safe levels.
We have a similar problem with the HRTEM (located ~30 meters from the NMR lab) in that a major high voltage conduit is located in the ceiling. As the EMF will drop with the square of the distance, the ~2 meter separation from conduit to TEM column is (just) sufficient to permit unimpeded operation.
The main point to take away from this is that any space MUST be checked for fields and vibrations before installing any microscope. This should be required by the TEM maker. There may be sources of EMF that are not obvious.
Cheers Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
} From: {hstahlberg-at-me.com} } Reply-To: {hstahlberg-at-me.com} } Date: Fri, 28 Jan 2011 04:40:50 -0600 } To: {raristau-at-ims.uconn.edu} } Subject: [Microscopy] How close can a NMR machine be installed next to a TEM } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Hi, } } How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year } old NMR machine? If both are on the same floor, then the magnetic field lines } from the NMR should run through the TEM column vertically, so that the } sensitivity for the strong DC fields of the NMR machine might be less ? } } I would appreciate any experience or recommendations, also directly towards } me. } } Thanks, } } Henning. } } ___________________________________________________ } } Henning Stahlberg, PhD. } Center for Cellular Imaging and Nano Analytics (C-CINA) } Prof. for Structural Biology } Biozentrum, University Basel } at the Department of Biosystems Science and Engineering (D-BSSE) } Mattenstrasse 26, WRO-1058 } CH-4058 Basel, Switzerland } Tel: +41-61-387 32 62 (office) } Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant) } Fax: +41-61-387 39 86 } mailto:Henning.Stahlberg-at-unibas.ch } Skype:henningstahlberg } http://c-cina.org } http://2dx.org } } Postal Address for delivery of Letters / Goods / Express Carriers: } C-CINA, Biozentrum University of Basel } c/o Syngenta AG, WRO-1058.6.60, D-BSSE } Schwarzwaldallee 215 } Postfach } CH-4002 Basel } ___________________________________________________ } } ==============================Original Headers============================== } 10, 23 -- From hstahlberg-at-me.com Fri Jan 28 04:36:31 2011 } 10, 23 -- Received: from asmtpout020.mac.com (asmtpout020.mac.com } [17.148.16.95]) } 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p0SAaVKt022583 } 10, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 04:36:31 -0600 } 10, 23 -- MIME-version: 1.0 } 10, 23 -- Content-transfer-encoding: 7BIT } 10, 23 -- Content-type: text/plain; CHARSET=US-ASCII } 10, 23 -- Received: from [192.168.1.35] ([92.104.21.174]) } 10, 23 -- by asmtp020.mac.com (Oracle Communications Messaging Exchange } Server 7u4-20.01 } 10, 23 -- 64bit (built Nov 21 2010)) with ESMTPSA id } {0LFQ009UNARUIS90-at-asmtp020.mac.com} } 10, 23 -- for Microscopy-at-microscopy.com; Fri, 28 Jan 2011 02:36:31 -0800 } (PST) } 10, 23 -- X-Proofpoint-Virus-Version: vendor=fsecure } 10, 23 -- engine=2.50.10432:5.2.15,1.0.148,0.0.0000 } 10, 23 -- definitions=2011-01-28_04:2011-01-28,2011-01-28,1970-01-01 } signatures=0 } 10, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 } spamscore=0 } 10, 23 -- ipscore=0 suspectscore=1 phishscore=0 bulkscore=0 adultscore=0 } classifier=spam } 10, 23 -- adjust=0 reason=mlx engine=6.0.2-1012030000 } definitions=main-1101280028 } 10, 23 -- From: Henning Stahlberg {hstahlberg-at-me.com} } 10, 23 -- Subject: How close can a NMR machine be installed next to a TEM } 10, 23 -- Date: Fri, 28 Jan 2011 11:35:53 +0100 } 10, 23 -- Message-id: {A81EC18B-D20C-4687-9FEA-E77A009400F7-at-me.com} } 10, 23 -- To: Microscopy-at-microscopy.com } 10, 23 -- X-Mailer: Apple Mail (2.1082) } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 25 -- From raristau-at-ims.uconn.edu Fri Jan 28 11:02:54 2011 8, 25 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SH2rNh026964 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 11:02:53 -0600 8, 25 -- Received: from [137.99.20.234] (d20h234.public.uconn.edu [137.99.20.234]) 8, 25 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id A66B92DF9A; 8, 25 -- Fri, 28 Jan 2011 12:02:52 -0500 (EST) 8, 25 -- User-Agent: Microsoft-Entourage/11.4.0.080122 8, 25 -- Date: Fri, 28 Jan 2011 12:02:50 -0500 8, 25 -- Subject: Re: [Microscopy] How close can a NMR machine be installed next to 8, 25 -- a TEM 8, 25 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 8, 25 -- To: {hstahlberg-at-me.com} , 8, 25 -- "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 8, 25 -- Message-ID: {C96860EA.22E5%raristau-at-ims.uconn.edu} 8, 25 -- Thread-Topic: [Microscopy] How close can a NMR machine be installed next 8, 25 -- to a TEM 8, 25 -- Thread-Index: Acu/DTG6cBV6NCsAEeC6lgAbY55CVA== 8, 25 -- In-Reply-To: {201101281040.p0SAeoxM028470-at-ns.microscopy.com} 8, 25 -- Mime-version: 1.0 8, 25 -- Content-type: text/plain; 8, 25 -- charset="US-ASCII" 8, 25 -- Content-transfer-encoding: 7bit 8, 25 -- X-Virus-Scanned: clamav-milter 0.96.1 at mta1 8, 25 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I would be interested in a summary of the answers received on this. We have a new instrument that may be installed within 200feet of an older model Varian MRI system, it is a superconductor, and the magnet is 4.7T. I do not believe that it is shielded the way the more modern MRIs are so I am curious what the field is out at 200ft from the instrument. I have the expressions for the fields from a solenoid at a distance, but I would rather have measurements than guesstimates, particularly when the kinds of static fields that we wish to avoid are probably in the milligauss or better regime. I will probably find an instrument (MRI) is operational and plot the fields as a function of both distance and angle to the principal axis of the MRI.
-- John Mansfield PhD Cphys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° 42.6439') AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
==============================Original Headers============================== 10, 20 -- From jfmjfm-at-umich.edu Fri Jan 28 12:25:29 2011 10, 20 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SIPSj3028806 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 12:25:28 -0600 10, 20 -- Received: FROM [141.212.214.158] (Unknown [141.212.214.158]) 10, 20 -- By hellskitchen.mr.itd.umich.edu ID 4D430A15.63C08.15230 ; 10, 20 -- Authuser jfmjfm; 10, 20 -- 28 Jan 2011 13:25:25 EST 10, 20 -- Subject: Re: [Microscopy] RE: How close can a NMR machine be installed next to a 10, 20 -- Mime-Version: 1.0 (Apple Message framework v1082) 10, 20 -- Content-Type: text/plain; charset=iso-8859-1 10, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} 10, 20 -- In-Reply-To: {201101281536.p0SFa58Y021495-at-ns.microscopy.com} 10, 20 -- Date: Fri, 28 Jan 2011 13:25:23 -0500 10, 20 -- Message-Id: {8999101F-6C91-4A0D-B53D-645FF0B7700A-at-umich.edu} 10, 20 -- References: {201101281536.p0SFa58Y021495-at-ns.microscopy.com} 10, 20 -- To: microscopy-at-microscopy.com 10, 20 -- X-Mailer: Apple Mail (2.1082) 10, 20 -- Content-Transfer-Encoding: 8bit 10, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0SIPSj3028806 ==============================End of - Headers==============================
I have a hard time getting the triglycerides to dye in 1 micron green algae due to their thick cell wall. Any suggestions? I plan to view this on a confocal laser scanning microscope.
Thanks!
==============================Original Headers============================== 5, 21 -- From dmywong-at-ucdavis.edu Fri Jan 28 14:36:09 2011 5, 21 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SKa7qA016884 5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 14:36:09 -0600 5, 21 -- Received: by yxl31 with SMTP id 31so1504926yxl.0 5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 12:36:06 -0800 (PST) 5, 21 -- Received: by 10.91.196.17 with SMTP id y17mr5625040agp.207.1296246965726; 5, 21 -- Fri, 28 Jan 2011 12:36:05 -0800 (PST) 5, 21 -- Received: from [192.168.1.101] ([128.120.242.213]) 5, 21 -- by mx.google.com with ESMTPS id w4sm22209508anw.36.2011.01.28.12.36.04 5, 21 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 5, 21 -- Fri, 28 Jan 2011 12:36:04 -0800 (PST) 5, 21 -- Message-Id: {012F709C-C0D0-42BE-8157-89CF50915E61-at-ucdavis.edu} 5, 21 -- From: "Diana M. Wong" {dmywong-at-ucdavis.edu} 5, 21 -- To: Microscopy-at-microscopy.com 5, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 5, 21 -- Subject: CLSM: Dying Triglyceride of microalgae 5, 21 -- Date: Fri, 28 Jan 2011 12:36:02 -0800 5, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
I am trying to do quantification of my cells by measuring the diameter of the fluorescence from a 3D construction image. I have heard of ImageJ, but I am not sure what pluggins to install. Have you tried a better program? ______________________________ Diana M. Wong Graduate Student
Franz Group University of California, Davis Department of Chemistry One Shields Ave Davis, CA 95616 dmywong-at-ucdavis.edu http://chemgroups.ucdavis.edu/~franz/
==============================Original Headers============================== 6, 21 -- From dmywong-at-ucdavis.edu Fri Jan 28 14:41:43 2011 6, 21 -- Received: from mail-gw0-f41.google.com (mail-gw0-f41.google.com [74.125.83.41]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SKfhX7020432 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 14:41:43 -0600 6, 21 -- Received: by gwj22 with SMTP id 22so1541232gwj.0 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 12:41:42 -0800 (PST) 6, 21 -- Received: by 10.90.84.7 with SMTP id h7mr5396488agb.55.1296247301077; 6, 21 -- Fri, 28 Jan 2011 12:41:41 -0800 (PST) 6, 21 -- Received: from [192.168.1.101] ([128.120.242.213]) 6, 21 -- by mx.google.com with ESMTPS id g2sm2838313yhc.28.2011.01.28.12.41.39 6, 21 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 6, 21 -- Fri, 28 Jan 2011 12:41:40 -0800 (PST) 6, 21 -- Message-Id: {D290C485-7F5E-45F1-B542-F8FEFBA0E7EC-at-ucdavis.edu} 6, 21 -- From: "Diana M. Wong" {dmywong-at-ucdavis.edu} 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 21 -- Subject: Confocal LSM: Quantification software? ImageJ 6, 21 -- Date: Fri, 28 Jan 2011 12:41:37 -0800 6, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Rather than going with the a la carte method of installing plugins for ImageJ* you could try one of the prepackaged collections. MBF ImageJ is a good prepackaged selection of plugins (http://www.macbiophotonics.ca/imagej/) You could also try Fiji (http://pacific.mpi-cbg.de/wiki/index.php/Fiji).
Regards,
Mike
(*Comments made above represent personal opinion and do not represent formal endorsement of any particular product, company, or organization by NIST.)
-----Original Message----- X-from: dmywong-at-ucdavis.edu [mailto:dmywong-at-ucdavis.edu] Sent: Friday, January 28, 2011 3:58 PM To: Anderson, Michael
I am trying to do quantification of my cells by measuring the diameter of the fluorescence from a 3D construction image. I have heard of ImageJ, but I am not sure what pluggins to install. Have you tried a better program? ______________________________ Diana M. Wong Graduate Student
Franz Group University of California, Davis Department of Chemistry One Shields Ave Davis, CA 95616 dmywong-at-ucdavis.edu http://chemgroups.ucdavis.edu/~franz/
==============================Original Headers============================== 6, 21 -- From dmywong-at-ucdavis.edu Fri Jan 28 14:41:43 2011 6, 21 -- Received: from mail-gw0-f41.google.com (mail-gw0-f41.google.com [74.125.83.41]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SKfhX7020432 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 14:41:43 -0600 6, 21 -- Received: by gwj22 with SMTP id 22so1541232gwj.0 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 12:41:42 -0800 (PST) 6, 21 -- Received: by 10.90.84.7 with SMTP id h7mr5396488agb.55.1296247301077; 6, 21 -- Fri, 28 Jan 2011 12:41:41 -0800 (PST) 6, 21 -- Received: from [192.168.1.101] ([128.120.242.213]) 6, 21 -- by mx.google.com with ESMTPS id g2sm2838313yhc.28.2011.01.28.12.41.39 6, 21 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 6, 21 -- Fri, 28 Jan 2011 12:41:40 -0800 (PST) 6, 21 -- Message-Id: {D290C485-7F5E-45F1-B542-F8FEFBA0E7EC-at-ucdavis.edu} 6, 21 -- From: "Diana M. Wong" {dmywong-at-ucdavis.edu} 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 21 -- Subject: Confocal LSM: Quantification software? ImageJ 6, 21 -- Date: Fri, 28 Jan 2011 12:41:37 -0800 6, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
==============================Original Headers============================== 17, 28 -- From michael.anderson-at-nist.gov Fri Jan 28 15:13:10 2011 17, 28 -- Received: from smtp.nist.gov (rimp1.nist.gov [129.6.16.226]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SLD7Nq017047 17, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 15:13:09 -0600 17, 28 -- Received: from WSXGHUB2.xchange.nist.gov (wsxghub2.nist.gov [129.6.18.19]) 17, 28 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id p0SLDCwB015552 17, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 16:13:12 -0500 17, 28 -- Received: from MBCLUSTER.xchange.nist.gov ([fe80::d479:3188:aec0:cb66]) by 17, 28 -- WSXGHUB2.xchange.nist.gov ([129.6.18.19]) with mapi; Fri, 28 Jan 2011 17, 28 -- 16:12:57 -0500 17, 28 -- From: "Anderson, Michael" {michael.anderson-at-nist.gov} 17, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 17, 28 -- Date: Fri, 28 Jan 2011 16:12:58 -0500 17, 28 -- Subject: RE: [Microscopy] Confocal LSM: Quantification software? ImageJ 17, 28 -- Thread-Topic: [Microscopy] Confocal LSM: Quantification software? ImageJ 17, 28 -- Thread-Index: Acu/LgWG7ETKLKAUTzyH3+igftiZtAAANZvw 17, 28 -- Message-ID: {7E80450DE7474E4881E405C742AC0DEA11DAAB5B7F-at-MBCLUSTER.xchange.nist.gov} 17, 28 -- References: {201101282057.p0SKveHC015550-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201101282057.p0SKveHC015550-at-ns.microscopy.com} 17, 28 -- Accept-Language: en-US 17, 28 -- Content-Language: en-US 17, 28 -- X-MS-Has-Attach: 17, 28 -- X-MS-TNEF-Correlator: 17, 28 -- acceptlanguage: en-US 17, 28 -- Content-Type: text/plain; charset="us-ascii" 17, 28 -- MIME-Version: 1.0 17, 28 -- X-NIST-MailScanner: Found to be clean 17, 28 -- X-NIST-MailScanner-From: michael.anderson-at-nist.gov ==============================End of - Headers==============================
I'm doing some testing today. Please bear with the likely of some test messages.
Nestor =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 9700 S. Cass Ave. Argonne, Illinois 60439 USA Tel: 530-NES-TORZ (530-637-8679) Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow of the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory President: Microscopy Society of America ===========================================
==============================Original Headers============================== 11, 24 -- From zaluzec-at-aaem.amc.anl.gov Sat Jan 29 09:53:54 2011 11, 24 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0TFrrN4014522 11, 24 -- for {microscopy-at-microscopy.com} ; Sat, 29 Jan 2011 09:53:53 -0600 11, 24 -- Received: from localhost (localhost [127.0.0.1]) 11, 24 -- by aaem.amc.anl.gov (Postfix) with ESMTP id D74DE2343B3B; 11, 24 -- Sat, 29 Jan 2011 09:53:52 -0600 (CST) 11, 24 -- X-Virus-Scanned: amavisd-new at aaem.amc.anl.gov 11, 24 -- Received: from aaem.amc.anl.gov ([127.0.0.1]) 11, 24 -- by localhost (aem005.amc.anl.gov [127.0.0.1]) (amavisd-new, port 10024) 11, 24 -- with ESMTP id tsveAIPV-hiB; Sat, 29 Jan 2011 09:53:52 -0600 (CST) 11, 24 -- Received: from [206.69.208.22] (msdvpn005.msd.anl.gov [130.202.251.5]) 11, 24 -- by aaem.amc.anl.gov (Postfix) with ESMTPA id 5D2C22343B30; 11, 24 -- Sat, 29 Jan 2011 09:53:51 -0600 (CST) 11, 24 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 11, 24 -- Content-Type: text/plain; charset=us-ascii 11, 24 -- Content-Transfer-Encoding: 7bit 11, 24 -- Subject: Administrivia: Testing today 11, 24 -- Date: Sat, 29 Jan 2011 09:53:49 -0600 11, 24 -- Message-Id: {270AF016-187C-4DDF-B8AD-5D8BE1C695D7-at-aaem.amc.anl.gov} 11, 24 -- Cc: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 11, 24 -- To: microscopy-at-microscopy.com 11, 24 -- Mime-Version: 1.0 (Apple Message framework v1082) 11, 24 -- X-Mailer: Apple Mail (2.1082) ==============================End of - Headers==============================
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Email: reynolds-at-vt.edu Name: Bill Reynolds
Organization: Virginia Tech
Title-Subject: [Microscopy] Re: How close can a NMR machine be installed next to
Message: We have a FEG-STEM with a GIF that is located about 30 meters away from an NMR lab. Our microscope operates to the microscope manufacturer's resolution specs. As others have noted, what causes problems for TEMs are magnetic fields that change relatively quickly. These come from things like power lines, large motors, and electromagnetic contacts or switches. Static fields do not matter much at all because you can compensate for them (even when the field is associated with a ferromagnetic sample in the microscope). When our microscope was installed, the manufacturer measured the stray magnetic fields (the alternating fields that are easy to detect), but I don't think they even bothered measuring the static field. As an aside, the natural static field at the earth's surface is small, but it is not insignificant. Its magnitude varies from place to place, but it is in the vicinity of half a Gauss. All microscopes essentially work in a 500 milli Gauss static background.
The large fields associated with NMR instruments are also static, so they probably should not be considered equivalent to the "stray EMF fields" discussed in various microscope installation specs. In our lab, the magnet is a superconducting coil, and the weak fringes of the magnetic field extends to our microscope. However, since the field doesn't change (and we don't distort it by moving around large chunks of iron), our microscope does not notice the presence of the NMR. If the NMR magnet ever quenches or is turned off, we will probably be able to see a very small change in the microscope alignment, but that kind of event has not happened yet because the NMR magnet has been on continuously for a couple years.
I assume, rather than a simple chord on a 2D z slice, you want to measure a line between two points on different Z planes in the 3D z stack. If so also try and track down a recent version of the 3D software Volocity from Perkin Elmer [formally Improvision], I am sure there will be a few licences for the software about in one of the microscopy cores or research groups at the university. Volocity is pretty hot at 3D quantification, e.g. cellular component volumes etc.., and it shows them as pretty pictures & videos. Although expensive to buy, Volocity works well, gives good feedback on what it was measured, and has excellent email and telephone support in the UK, and I expect the same is true in the US. You will need to find a licenced user with at least the visualisation module, and probably the quantitation module [i.e. drawing tools], particularly if you want to take the image analysis further [these cost extra and not all users will have all the modules].
http://www.cellularimaging.com/products/volocity http://www.well.ox.ac.uk/_asset/file/volocity-manual.pdf [see line tool] http://www.cellularimaging.com/lab_of_the_month/detail.php?id=12
I'm sure there will be other microscope software that other Californian University groups may have that can do this as well, including ImageJ [although I can't think of a 3D line distance measurements ImageJ app, more volume rendering, but check the Fuji version], MetaMorph [also only available via an optional 3D module] and Image-Pro Plus [3D rendering and measurement].
If you find your XY pixel cordinates of the first and last point in the 3D confocal z-stack and know your approx. optical slice thickness and number of slices, I guess you could calculate/estimate the connecting line length pretty easily [you'd convert everything to pixels and then back to um]. There'd be errors in the length measurement, e.g. owing to perhaps 10 to 25% reduction in size due to fixation, possible distortion effects and estimation of Z optical distances.
e.g. calculate using http://www.analyzemath.com/Geometry_calculators/distance_midpoint_3D.html
Also see: Microscopy Image Analysis and Processing Software links at: http://www.well.ox.ac.uk/external-website-links
Regards
Keith
No commercial interest
-------------------------------------------------
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
Dear microscopists, I have been asked to reproduce an apoptosis assay whereby cells in a dish cells are exposed to UVB light and then screened 8 to 36 hours later. Does anybody have experience with this who could give us some tips? May we use UVA, as in the UV lamp on the microscope, or do you have suggestions what type of lab might have a UVB lamp available? Thank you. Sincerely, Michael
_________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
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==============================Original Headers============================== 6, 29 -- From Michael.Cammer-at-med.nyu.edu Sun Jan 30 19:25:32 2011 6, 29 -- Received: from mail4.nyumc.org (mail4.nyumc.org [216.165.126.170]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0V1PWoF009546 6, 29 -- for {Microscopy-at-microscopy.com} ; Sun, 30 Jan 2011 19:25:32 -0600 6, 29 -- X-IronPort-Anti-Spam-Filtered: true 6, 29 -- X-IronPort-Anti-Spam-Result: ApsEANSdRU0KkyXk/2dsb2JhbAClaLBdiGiDEYI9BIUT 6, 29 -- X-IronPort-AV: E=Sophos;i="4.60,402,1291611600"; 6, 29 -- d="scan'208";a="113355490" 6, 29 -- Received: from msgwsdcpht62.nyumc.org ([10.147.37.228]) 6, 29 -- by mail4.nyumc.org with SMTP; 30 Jan 2011 20:25:31 -0500 6, 29 -- Received: from MSGWSDCPMB06.nyumc.org ([10.147.55.93]) by 6, 29 -- MSGWSDCPHT62.nyumc.org ([10.147.37.228]) with mapi; Sun, 30 Jan 2011 20:25:31 6, 29 -- -0500 6, 29 -- From: "Cammer, Michael" {Michael.Cammer-at-med.nyu.edu} 6, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 29 -- Date: Sun, 30 Jan 2011 20:25:31 -0500 6, 29 -- Subject: how to induce apoptosis for in vitro microscopy? 6, 29 -- Thread-Topic: how to induce apoptosis for in vitro microscopy? 6, 29 -- Thread-Index: AQHLwOW/gnV38X+oNUGrJiYV3UT/IQ== 6, 29 -- Message-ID: {725986F6C4F57449A27E7D10B49F82B3151350C7CA-at-MSGWSDCPMB06.nyumc.org} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- acceptlanguage: en-US 6, 29 -- Content-Type: text/plain; charset="us-ascii" 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0V1PWoF009546 ==============================End of - Headers==============================
Dear All, I would like to do some work on lichen. Now I got access to a huge herbarium collection of lichen. What it do any good to use these room-dried specimen, moisture them, fix and go through ethanol to critcal point drying? Any experience with this out there?
Best wishes, Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: ian.maclaren-at-glasgow.ac.uk Name: Ian MacLaren
Organization: University of Glasgow
Title-Subject: [Filtered] EMAG2011 - Abstract Submission Open
Message: EMAG2011 - Quantifying the Nanoworld September 6-9 2011 University of Birmingham, UK
Call for abstracts
The biennial EMAG conference will this year be held at the University of Birmingham and promises to be an excellent meeting. Highlights of the programme will include plenary talks from Dr Frances Ross, Prof. Knut Urban and Prof. Richard Henderson, together with an exciting range of invited talks giving a snapshot of many current directions in electron microscopy and its applications. There will also be plenty of opportunity at this meeting to present papers orally or as posters, and research students are especially encouraged to present their work at this meeting. Scientific sessions are expected to include:
* Nanofabrication and scanning electron microscopy * Modelling and quantification * Advances in imaging and spectroscopy techniques * Applications of high resolution imaging and spectroscopy * Functional materials * Nanomaterials * Structural materials * Biosciences, biomaterials and soft matter * Earth and environmental sciences * In-situ electron microscopy Abstract submission is now open via the conference website at:
http://www.emag-iop.org/ and the closing date for abstract submission is 2 March 2011. Further information about the conference is also available from the same website. We look forward to seeing many of you in Birmingham in September.
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Email: vcsolomon-at-ysu.edu Name: Virgil C. Solomon
Organization: Youngstown State University
Title-Subject: [Filtered] Postdoctoral Research Position
Message: A postdoctoral research position is available at Youngstown State University to study ceramic-metallic composites obtained by reactive melt infiltration. More specifically, the successful candidate will use transmission and scanning electron microscopy methods to characterize micro- and nanostructural features of the composite materials. Candidates should have a Ph.D. in materials science, chemistry, physics or related engineering discipline. The position requires extensive experience in analytical scanning and transmission electron microscopy and sample preparation techniques; additional experience working with focused ion beam is preferred. Responsibilities of the successful candidate will include planning and performing experiments, processing and evaluating the results, and preparing scientific reports and journal publications. This position provides the opportunity to work in a new, fully-equipped, state-of-the-art electron microscopy facility which opened at YSU in 2010.
More information available at http://www.ysu.edu/hr/positions.shtml
Interested candidates can apply by email or by sending a cover letter, CV with a complete list of publications, and contact information of 3 professional references to: Dr. Tim Wagner or Dr. Virgil Solomon Youngstown State University One University Plaza Youngstown OH 44555-0001 E-mails: trwagner-at-ysu.edu; vcsolomon-at-ysu.edu
it's not my particular area of expertise but I do recall that some of our researchers used to "revitalise" their dried lichen samples by simply putting them on a damp filter paper in a petri-dish. If you think about it, this is probably what happens in nature (except for the filter paper etc).
I seem to recall that this process worked with most specimens but depended on the original drying, storage and presumably the resilience of the fungi and algae symbionts.
Good luck
Malcolm
Malcolm Haswell Imaging Suite Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: stefan.diller-at-t-online.de
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Email: steven.samuelsson-at-sri.com Name: Steven Samuelsson Organization: SRI International, Inc.
Message: We recently acquired a vintage Reichert- Jung, Cryocut 1800. Unfortunately, it is missing much of the knife holder assemble. Does anyone on the listserver have these parts and are willing to sell or surplus them? The unit is in good condition and should serve us well. Thanks so much.
Steve -- Steven Samuelsson, PhD Director Cell and Molecular Imaging Facility 100-51 SRI International, Inc. 333 Ravenswood Ave Menlo Park, CA 94061 (650) 859-2980
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Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: Skirball Institute of Biomolecular Medicine
Title-Subject: [Filtered] Type K(?) insert question
Message: We want to built a custom type K (?), 110 X 160 mm with rounded corners, insert for our Zeiss microscope stage that will allow us to insert another stage insert approximately 2 cm higher than the current stage.
Does anyone know where we can get drawings of the insert that we modify for a machine shop?
Or does anyone know if such a stage height extender already exists?
Thank you very much.
Sincerely,
Michael
________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
The Hooke College of Applied Sciences, located in Westmont, IL, is offering the following electron microscopy short courses:
March 7 to 11- Scanning Electron Microscopy
March 15 to 17 - Transmission Electron Microscopy
In addition to lectures, these courses emphasize hands-on training using state of the art equipment. For further details and registration information, please follow the link below:
http://www.hookecollege.com/
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
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==============================Original Headers============================== 15, 24 -- From eschumacher-at-mccrone.com Tue Feb 1 09:42:05 2011 15, 24 -- Received: from mail.mccrone.com (mail.mccrone.com [12.54.22.114]) 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p11Fg4WM011955 15, 24 -- for {microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 09:42:05 -0600 15, 24 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by 15, 24 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Tue, 1 Feb 2011 09:42:02 15, 24 -- -0600 15, 24 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 15, 24 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 15, 24 -- Date: Tue, 1 Feb 2011 09:42:02 -0600 15, 24 -- Subject: Short Course Announcement: SEM and TEM 15, 24 -- Thread-Topic: Short Course Announcement: SEM and TEM 15, 24 -- Thread-Index: AcvCJpHK9zItNqU0QweSJMKLY4Mzdw== 15, 24 -- Message-ID: {874B1DB532886444A60A015EE363493F4849CECF32-at-mccronemsg07.tmg.mccrone.com} 15, 24 -- Accept-Language: en-US 15, 24 -- Content-Language: en-US 15, 24 -- X-MS-Has-Attach: 15, 24 -- X-MS-TNEF-Correlator: 15, 24 -- x-vipre-scanned: 05BFDCEF00205105BFDE3C 15, 24 -- acceptlanguage: en-US 15, 24 -- Content-Type: text/plain; charset="us-ascii" 15, 24 -- MIME-Version: 1.0 15, 24 -- Content-Transfer-Encoding: 8bit 15, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p11Fg4WM011955 ==============================End of - Headers==============================
Dear Stefan, I have no specific experience with lichen but I suspect that whatever structural damage occurred by air drying cannot be repaired by rehydration. I bet you can take small sample of the herbarium specimen and put it on a stub and examine directly. I think you can test it first in a vacuum chamber (for example of a good coater) and see if it outgasses much. Probably it won't. Insofar as lichens air dry in nature and plant and fungal cell walls are tough, it is reasonable to examine air dried material in lieu of fresh samples.
Clearly if as Malcolm Haswell suggests the herbarium specimen is still living, then that gives you the chance to work with fresh material. But I wonder what the odds of that are?
As ever Tobias Baskin
} } } Dear All, } I would like to do some work on lichen. Now I got access to a huge } herbarium collection of lichen. } What it do any good to use these room-dried specimen, moisture them, } fix and go through ethanol to critcal point drying? } Any experience with this out there? } } Best wishes, } Stefan } } -- } ----------------------------------------------------- } Stefan Diller - Scientific Photography } Arndtstrasse 22 } D - 97072 Wuerzburg Germany } ++49-931-7848700 Phone } ++49-931-7848701 Fax } ++49-175-7177051 Mobile } } Websites: } www.stefan-diller.com } www.elektronenmikroskopie.info } www.assisi.de } www.zwillingsprojekt.de } Anfahrt: //Mail.map24.com/Stefan.Diller } ----------------------------------------------------- } } =
We often neglect to consider that when something has already been dried 'naturally' there may be no reason the redo the process 'un-naturally'. Especially in the cases of organisms that can survive dehydration by re-animating when re-hydrated. There is a roundworm in S. America that survives complete dessication in the dried mud - waiting for reanimation in the next rainy season. (Could NOT quickly find a reference, but I first saw this in a text - when I was young - in the early 1960's.)
This is especially true, if you have a variable pressure SEM (ESEM) at hand.
Further, after critical point drying, we also fail to recognize that the 'almost completely dry' natural material has been remade into a quite hygroscopic material that will rehydrate while we carry it to the microscope or coater (evacuate (shrinkage) and coat, then re-pressurize, hydrate and stretch!). All of this was invented to permit us to view free-standing microvilli as well as other delicate cell surface features.
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: Tuesday, February 01, 2011 7:32 AM To: Monson, Frederick
Dear All, I would like to do some work on lichen. Now I got access to a huge herbarium collection of lichen. What it do any good to use these room-dried specimen, moisture them, fix and go through ethanol to critcal point drying? Any experience with this out there?
Best wishes, Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Hi all, Does anyone have a favorite TEM protocol for fixing mouse cornea that they'd be willing to share? The tissue we have is infected with a fungus so we'd like to have good preservation of both. TIA and my best, Beth
Beth Richardson EM Lab Coordinator Plant Biology Dept. University of Georgia Athens, GA 30602
==============================Original Headers============================== 5, 19 -- From beth-at-plantbio.uga.edu Tue Feb 1 13:55:21 2011 5, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p11JtIPe001424 5, 19 -- for {microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 13:55:20 -0600 5, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 5, 19 -- (authenticated user beth-at-plantbio.uga.edu) 5, 19 -- by dogwood.plantbio.uga.edu 5, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 5, 19 -- for microscopy-at-microscopy.com; 5, 19 -- Tue, 1 Feb 2011 14:55:14 -0500 5, 19 -- Message-Id: {1135C2EB-B555-4DD6-833D-1D690BEB1955-at-plantbio.uga.edu} 5, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Mime-Version: 1.0 (Apple Message framework v936) 5, 19 -- Subject: fixing mouse cornea 5, 19 -- Date: Tue, 1 Feb 2011 14:55:14 -0500 5, 19 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
The editor of a forthcoming Wiley-Blackwell book on Biofouling Methods is looking for someone to write a chapter on electron microscopy methods. This is expected to be a brief literature review, with an introduction about methods and some actual protocols, designed for beginners in the field. If you are interested, please contact Sergey Dobretsov at sergey_dobretsov-at-yahoo.com
Aloha from sunny and warm Hawaii (had to rub it in), Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Tue Feb 1 15:06:27 2011 4, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p11L6Q2R019293 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 15:06:26 -0600 4, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p11L6PH1008231 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 11:06:25 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p11L6O1Q008227 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 11:06:25 -1000 (HST) 4, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Tue, 1 Feb 2011 11:06:24 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-b1000 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: Need a chapter on EM for a book 4, 20 -- Message-ID: {Pine.GSO.4.64.1102011104350.5800-at-b1000} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
As suggested already, if you have access to a VP-SEM, all you need to do is put a piece on a stub and image. And sometimes BSE is better than SE, or a combination of the two can work well. And in a VP-SEM, you don't need to worry about outgassing so much.
After that, you could try looking at a hydrated specimen in VP mode, with water as the gas, if your SEM has that capacity.
The entomologists across the road are big fans of the VP-SEM, because it means they can now look at details of their precious type specimens, especially the very tiny ones, none of which are allowed to be coated with anything.
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 1/02/11 11:29 PM, "stefan.diller-at-t-online.de" {stefan.diller-at-t-online.de} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } I would like to do some work on lichen. Now I got access to a huge herbarium } collection of lichen. } What it do any good to use these room-dried specimen, moisture them, fix and } go through ethanol to critcal point drying? } Any experience with this out there? } } Best wishes, } Stefan
==============================Original Headers============================== 11, 42 -- From prvs=006eee44e=Rosemary.White-at-csiro.au Tue Feb 1 15:34:52 2011 11, 42 -- Received: from vic-ironport-ext.csiro.au (vic-mx-ext.csiro.au [150.229.64.36]) 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p11LYp8t003304 11, 42 -- for {microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 15:34:52 -0600 11, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 11, 42 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 11, 42 -- s=email; t=1296596092; x=1328132092; 11, 42 -- h=date:subject:from:to:cc:message-id:in-reply-to: 11, 42 -- mime-version:content-transfer-encoding; 11, 42 -- z=Date:=20Wed,=202=20Feb=202011=2008:34:49=20+1100 11, 42 -- |Subject:=20Re:=20[Microscopy]=20SEM=20prep=20of=20lichen 11, 42 -- =20from=20a=20herbarium|From:=20Rosemary=20White=20 {rosem 11, 42 -- ary.white-at-csiro.au} |To:=20"stefan.diller-at-t-online.de"=20 { 11, 42 -- stefan.diller-at-t-online.de} |CC:=20 {microscopy-at-microscopy.c 11, 42 -- om} |Message-ID:=20 {C96EC7A9.21197%rosemary.white-at-csiro.au 11, 42 -- } |In-Reply-To:=20 {201102011229.p11CTq4x016236-at-ns.microsco 11, 42 -- py.com} |MIME-Version:=201.0|Content-Transfer-Encoding:=20 11, 42 -- 7bit; 11, 42 -- bh=9TRDFqpqRN1bdVNvOFDlMx5U/Gk9blzeTG1TonXHvE4=; 11, 42 -- b=HWF8t3R2/h16Lp0f9PVok799pYD36NMLIe+idDXMmZjQSbn9j9exnpks 11, 42 -- RxlMhigmFaLwiWqZydWatdvJzGtN2LHOckHOaermHc/CfO7EL5IFEYGpS 11, 42 -- lUV+OWtYBuaO2uP; 11, 42 -- X-IronPort-AV: E=Sophos;i="4.60,411,1291554000"; 11, 42 -- d="scan'208";a="54545709" 11, 42 -- Received: from exvic-htca02.nexus.csiro.au ([138.194.81.127]) 11, 42 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 02 Feb 2011 08:34:50 +1100 11, 42 -- Received: from [152.83.195.117] (152.83.195.117) by 11, 42 -- exvic-htca02.nexus.csiro.au (138.194.81.127) with Microsoft SMTP Server (TLS) 11, 42 -- id 8.1.436.0; Wed, 2 Feb 2011 08:34:22 +1100 11, 42 -- User-Agent: Microsoft-Entourage/12.28.0.101117 11, 42 -- Date: Wed, 2 Feb 2011 08:34:49 +1100 11, 42 -- Subject: Re: [Microscopy] SEM prep of lichen from a herbarium 11, 42 -- From: Rosemary White {rosemary.white-at-csiro.au} 11, 42 -- To: "stefan.diller-at-t-online.de" {stefan.diller-at-t-online.de} 11, 42 -- CC: {microscopy-at-microscopy.com} 11, 42 -- Message-ID: {C96EC7A9.21197%rosemary.white-at-csiro.au} 11, 42 -- Thread-Topic: [Microscopy] SEM prep of lichen from a herbarium 11, 42 -- Thread-Index: AcvCC6tpNkA5cKiQSneyVPbN+dFh2wATC7YU 11, 42 -- In-Reply-To: {201102011229.p11CTq4x016236-at-ns.microscopy.com} 11, 42 -- MIME-Version: 1.0 11, 42 -- Content-Type: text/plain; charset="US-ASCII" 11, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We have been offered a demonstration in our laboratory of the Milestone "KOS EM" tissue processor. Has anyone encountered this product before? Perhaps some of you have had experience with other Microwave tissue processors that you may wish to share.
Thanks in advance Naomi
Naomi McCallum Supervising Scientist, EM Unit, Anatomical Pathology Pathology Queensland _________________________________________________ Clinical and Statewide Services Division| Queensland Health
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==============================Original Headers============================== 9, 25 -- From prvs=101452c9f9=naomi_mccallum-at-health.qld.gov.au Tue Feb 1 21:00:53 2011 9, 25 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1230q5x025783 9, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 21:00:53 -0600 9, 25 -- Received: from gwd-mail06.remote.health.qld.gov.au (gwd-mail06.remote.health.qld.gov.au [192.168.3.53]) 9, 25 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id p1230BBR007259 9, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 13:00:11 +1000 9, 25 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 9, 25 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP Server) 9, 25 -- with ESMTP id 9, 25 -- {T9af2de31c6c0a80335d48-at-gwd-mail06.remote.health.qld.gov.au} for 9, 25 -- {Microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 13:00:11 +1000 9, 25 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 9, 25 -- Novell_GroupWise; Wed, 02 Feb 2011 13:00:10 +1000 9, 25 -- Message-Id: {4D495547.88BE.00AA.0-at-health.qld.gov.au} 9, 25 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 9, 25 -- Date: Wed, 02 Feb 2011 12:59:54 +1000 9, 25 -- From: "Naomi McCallum" {naomi_mccallum-at-health.qld.gov.au} 9, 25 -- To: {Microscopy-at-microscopy.com} 9, 25 -- Subject: KOS EM (Milestone) Microwave Tissue Processor 9, 25 -- Mime-Version: 1.0 9, 25 -- Content-Type: text/plain; charset="us-ascii" 9, 25 -- Content-Disposition: inline 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1230q5x025783 ==============================End of - Headers==============================
Dear Justin, We can supply small quantities of Pt-Ir STM tips at a price much lower than "$200 per tip". The tips we have were manufactured at Digital Instruments for use with their STM. Please contact me offline and share details of the wire size requirements of the Easyscan stm.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] 2009-2010 is our 20th year in business! ============================================= ----- Original Message ----- From: kraftpiano-at-gmail.com To: donc-at-asmicro.com Sent: Friday, January 21, 2011 12:10 AM Subject: [a] [Microscopy] STM tip advice.
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I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf).
I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution.
The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses.
Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 6, 37 -- From kraftpiano-at-gmail.com Thu Jan 20 23:08:26 2011 6, 37 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L58Q25003006 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 23:08:26 -0600 6, 37 -- Received: by yxl31 with SMTP id 31so469145yxl.0 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 21:08:26 -0800 (PST) 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 37 -- d=gmail.com; s=gamma; 6, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding 6, 37 -- :subject:date:message-id:to:mime-version:x-mailer; 6, 37 -- bh=0UOwJelpZ6QJtvR6h504fJNBtAxqg11VNSbyg5wJQ74=; 6, 37 -- b=CKvCVRNoVTsFwRnLpU5lCXf8YvPMxH1N3dS7ys8UPf3yiqhPDWGbMo0obmG88cgOgo 6, 37 -- XZULU3FfCYHsarDBM9jexEC0W3v5YP7EFDMCVYhQlXG/1kA7Yhd73anJHaX3ZIz+H7uz 6, 37 -- JgDVe+ofiVTeCAAmXGLSf7iUKv6XvcnYIVDww= 6, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 37 -- d=gmail.com; s=gamma; 6, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id 6, 37 -- :to:mime-version:x-mailer; 6, 37 -- b=PR17AmFsaRKhUKvApaUTF9Rk1PZybTM2uGPbnyI+JSfekwjDyixYQTfQWeBGOPBeUR 6, 37 -- jtKbSKxwwTpYoyFNLlhFZrNVcU8rUujb+K2L3feJgIONXADzcGx34Xj28IMbBnWZHLwQ 6, 37 -- 1EKfQrQ/cpSgDSCYg9bEB0VVr0WIDudjYmUZI= 6, 37 -- Received: by 10.151.68.12 with SMTP id v12mr198668ybk.387.1295586505917; 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) 6, 37 -- Received: from [10.101.1.3] (c-98-249-167-116.hsd1.fl.comcast.net [98.249.167.116]) 6, 37 -- by mx.google.com with ESMTPS id u10sm82704yba.13.2011.01.20.21.08.24 6, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) 6, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} 6, 37 -- Content-Type: text/plain; charset=us-ascii 6, 37 -- Subject: STM tip advice. 6, 37 -- Date: Fri, 21 Jan 2011 00:08:23 -0500 6, 37 -- Message-Id: {EA4CFA28-451C-4E4D-A543-EEA026824B89-at-gmail.com} 6, 37 -- To: microscopy-at-microscopy.com 6, 37 -- Mime-Version: 1.0 (Apple Message framework v1082) 6, 37 -- X-Mailer: Apple Mail (2.1082) 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0L58Q25003006 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 37 -- From donc-at-asmicro.com Tue Feb 1 21:16:05 2011 15, 37 -- Received: from nm18.bullet.mail.sp2.yahoo.com (nm18.bullet.mail.sp2.yahoo.com [98.139.91.88]) 15, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p123G45i006759 15, 37 -- for {microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 21:16:05 -0600 15, 37 -- Received: from [98.139.91.69] by nm18.bullet.mail.sp2.yahoo.com with NNFMP; 02 Feb 2011 03:16:04 -0000 15, 37 -- Received: from [98.139.91.51] by tm9.bullet.mail.sp2.yahoo.com with NNFMP; 02 Feb 2011 03:16:04 -0000 15, 37 -- Received: from [127.0.0.1] by omp1051.mail.sp2.yahoo.com with NNFMP; 02 Feb 2011 03:16:04 -0000 15, 37 -- X-Yahoo-Newman-Id: 507173.81244.bm-at-omp1051.mail.sp2.yahoo.com 15, 37 -- Received: (qmail 69479 invoked from network); 2 Feb 2011 03:16:04 -0000 15, 37 -- Received: from asm15 (donc-at-98.226.79.148 with login) 15, 37 -- by smtp105.sbc.mail.gq1.yahoo.com with SMTP; 01 Feb 2011 19:16:00 -0800 PST 15, 37 -- X-Yahoo-SMTP: 9Mfb0weswBDvm9orSc8mC.8UiMpq8hJTCpyHEMOqu04urw-- 15, 37 -- X-YMail-OSG: OwyFFLQVM1l627x6tANLqy9dAhznmGz_RYKL37RoMqudbgD 15, 37 -- sjMM_zwbMpL4jA.5BJfq0enNJKlVVzd2uPKjZXt791Ujqn0GzXGLw1ZDNG5R 15, 37 -- K.FNTnLyj8CQ1l.J7.e4bvJR7FEgciIkD6L.9aI0MbUiyjux414wQSUT1jzS 15, 37 -- JCEM9JVgPHEt_gkSB9qOFeq5TUhFJDPIL80GCC2LoZQ1u9E8FvKe64DRZVLz 15, 37 -- GL43xGl_vYp4W4YZ7QK2zSSqYeEtHTA6jfWwDI6rBCkCYWyCe.nI1A4zq1z1 15, 37 -- buvs3WvOXPXjz1blFJP8WHeJoDmFSCfWzkU0Gzi_T6PTHZBFiDPtk1GKcO5Z 15, 37 -- cs52bx8rES9VmSyqnIqOEkGl_DKkatNWZOcUBtB.MrBc- 15, 37 -- X-Yahoo-Newman-Property: ymail-3 15, 37 -- Message-ID: {F04A2847377549D2AF7EA247052A4897-at-asm15} 15, 37 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 15, 37 -- To: {kraftpiano-at-gmail.com} 15, 37 -- Cc: "Microscopy List" {microscopy-at-microscopy.com} 15, 37 -- References: {201101210510.p0L5A0cD006982-at-ns.microscopy.com} 15, 37 -- Subject: Re: [a] [Microscopy] STM tip advice. - Commercial reply 15, 37 -- Date: Tue, 1 Feb 2011 22:14:14 -0500 15, 37 -- MIME-Version: 1.0 15, 37 -- Content-Type: text/plain; 15, 37 -- format=flowed; 15, 37 -- charset="iso-8859-1"; 15, 37 -- reply-type=original 15, 37 -- Content-Transfer-Encoding: 7bit 15, 37 -- X-Priority: 3 15, 37 -- X-MSMail-Priority: Normal 15, 37 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 15, 37 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 ==============================End of - Headers==============================
Sorry for the delay in response to this topic, but due to an error on my on my part my previous communication went astray. For those who may be interested in the history of ultramicrotomy I do have a copy of an article in PDF form which traces the development of the ultramicrotome from "wedge" sections in the late thirties where the thin end of the wedge was (hopefully) transparent to electrons, through the high speed era (actually up to 57000 rpm), overcoming embedding limitations and finally discussing the first generation of commercial instruments.
Please contact me on terry.cooper-at-btinternet.com and I can attach the missive,
Best regards
Terry Cooper TAAB Laboratories Equipment 3 Minerva House, Calleva Park Aldermaston, Berks, RG7 8NA, England Tel ++44(0)118 981 7775 Fax ++44(0)118 981 7881 e-mail sales-at-taab.co.uk www.taab.co.uk
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Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College at UPEI
Title-Subject: [Filtered] SEM sample storing
Message: Hello all, One of our EM lab users asked me a question: what is the best way to store SEM samples? I know you'll all say that the best is to process and coat first. He wants to store them in 2% glutaraldehyde and process later since he does not have time at the moment. What is your experience? I am not an expert in SEM. Thank you Dorota Login Host: 137.149.102.148 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 24 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 2 07:55:51 2011 7, 24 -- Received: from hiltonsmtp.worldspice.net (hiltonsmtp.worldspice.net [216.37.94.58]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12DtpQn024776 7, 24 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 07:55:51 -0600 7, 24 -- Received: (qmail 2812 invoked by uid 0); 2 Feb 2011 13:55:50 -0000 7, 24 -- Received: by simscan 1.4.0 ppid: 2527, pid: 2784, t: 0.4412s 7, 24 -- scanners: clamav: 0.94.1/m:50/d:9101 spam: 3.2.5 7, 24 -- X-Spam-Checker-Version: SpamAssassin 3.2.5-wspice_748 (2008-06-10) on 7, 24 -- hiltoncluster1.hiltonsmtp.worldspice.net 7, 24 -- X-Spam-Level: 7, 24 -- X-Spam-Status: No, score=0.9 Bayes=0.5 required=6.5 autolearn=disabled version=3.2.5-wspice_748 report= 7, 24 -- * 0.9 CS_796 CS_796 7, 24 -- Received: from unknown (HELO NestorPwrBk.local) (zaluzec-at-12.97.46.2) 7, 24 -- by hiltoncluster01.worldspice.net with ESMTPA; 2 Feb 2011 13:55:50 -0000 7, 24 -- Message-ID: {4D49625B.7080502-at-microscopy.com} 7, 24 -- Date: Wed, 02 Feb 2011 05:55:39 -0800 7, 24 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 24 -- Reply-To: wadowska-at-upei.ca 7, 24 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 24 -- MIME-Version: 1.0 7, 24 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 24 -- Subject: viaWWW:SEM sample storing 7, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Dorota To my experience specimens can stay in glutar for an indefinite time. I have processed samples after } 3 years stored in glutar inside the fridge, and they looked same as others being processed within a few days. In rare cases I've seen mushrooms developing in samples that stayed only a few weeks in glutar. There I suspect glutar was old, samples were dirty from the beginning, or some other unedintified factor was present. If you process and coat the specimens I think it is better to view them soon, otherwise can get humidified and morphology deteriorates. In conclusion I think it is not a bad idea to have your samples staying in glutar until you have the time to view them. With best wishes yorgos
----- Original Message ----- X-from: {microscopylistserver-noreply-at-microscopy.com} To: {eikonika-at-otenet.gr} Sent: Wednesday, February 02, 2011 3:05 PM
Dear Colleagues,
Openings for Engineers & Technicians in Physical Failure Analysis (PFA) are available in Global Foundries, R&D Center at East Fishkill, NY.
Engineers should be strong in FIB & SEM / basic TEM & device physics. Industry experience with device / wafer-fab helpful.
Technicians should have extensive hands-on skills in various TEM sample-prep techniques, e.g., delayering / mechanical polishing / FIB / CLM, etc. Previous experience at high-throughput environments preferred, but not a requirement.
Applicants should demonstrate unrestricted employment eligibility in USA, which usually requires US citizenship or green card.
If interested, please send a cover letter and resume, "offline", to wayne.zhao-at-globalfoundries.com .
Wayne
------------------------------------------- Wayne Zhao, PhD Technical Lead of PFA Team Sr. Member of Technical Staff R & D Center at East Fishkill, NY Global Foundries -------------------------------------------
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Email: bbandli-at-d.umn.edu Name: Bryan Bandli
Organization: University of Minnesota, Duluth
Title-Subject: [Filtered] HMDS, How does it work...
Message: Hi All,
I'm looking for a reference to explain just how/why HMDS works to dry specimens for SEM observation. There are plenty of references showing that it does, in fact, work and produces good results in many cases but I have yet to find an explanation as to the "why". Is it that HMDS has the right physical properties (surface tension) to evaporate without causing drying artifacts?
Or, is there something about the silanization of the sample that helps make it more robust, or both, or am I completely lost (quite likely)?
Bryan, I believe it has to do with very low surface tension. It also doesn't work well across the board. I understand that it works well with insects, but one of my sons did a science fair project comparing dried, freeze dried and HMDS treated green pepper. In his case the HMDS didn't appear to be any better than drying. His best results were from plunging small pieces into acetone cooled with a Peltier cooler, then transferring to another Peltier cooler in a vacuum evaporator until dehydrated. He was not able to compare it to CPD prep as we didn't have the requisite CO2. Obviously, the plunge freezing was not done at typical temperatures, so there was probably ice damage in those samples, but they looked the best of the bunch.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, February 02, 2011 10:02 AM To: kenconverse-at-qualityimages.biz
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Email: bbandli-at-d.umn.edu Name: Bryan Bandli
Organization: University of Minnesota, Duluth
Title-Subject: [Filtered] HMDS, How does it work...
Message: Hi All,
I'm looking for a reference to explain just how/why HMDS works to dry specimens for SEM observation. There are plenty of references showing that it does, in fact, work and produces good results in many cases but I have yet to find an explanation as to the "why". Is it that HMDS has the right physical properties (surface tension) to evaporate without causing drying artifacts?
Or, is there something about the silanization of the sample that helps make it more robust, or both, or am I completely lost (quite likely)?
Although I have HMDS and have used it, I'm no expert.
That said,
given the following pieces, I'd say the lower surface tension allows better permeation and the evaporation rate is near sublimatic in process because of both the lower surface tension and higher vapor pressure [the lower surface tension maximizes the area and the thinness of the film, both of which increase evaporation flux]:
1. Bray, Comparison of Hexamethyldisilazane (HMDS), Peldri 11, and Critical-Point Drying Methods for SEM of Biological Specimens, Microsc Res Tech, 26, 489-495, 1993.
"Recently, several drying methods which claim to alleviate some of the disadvantages of CPD have been described. One of these methods (Kennedy et al., 1989) is similar to freeze drying and utilizes a sublimation dehydrant, Peldri 11, which is frozen and then sublimed from specimens. The Peldri 11 procedure requires no special equipment but can take several hours to perform a run. Other methods involve the use of low-surface- tension solvents such as Hexamethyldisilazane (HMDS) (Nation, 1983), tetramethylsilane (TMS) (Dey et al., 19891, and dimethoxypropane (DMP) (Weyda, 1992). These solvents evaporate directly from tissue in minutes, and infiltration times usually take { 1 hr."
2. Beno, Processing of soft pupae and uneclosed pharate adults of Drosophila for scanning electron microscopy, Microsc Res Tech, 70, 1022-1027, 2007
"We found that it is crucial to leave minute amount of above used solvents (ethanols, acetone, and mixture of acetone: hexamethyldisilazane) in eppendorf tube prior to each exchange to prevent animal from fast drying and damage."
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon IN 46123 www.ph2llc.com (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
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-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, February 02, 2011 10:04 AM To: ph2-at-sprynet.com
Fascinating article. Some of my recollections (possibly with some inaccuracies but the best I can recollect) involve Fernandez-Moran who is credited with developing the first diamond knives for use with ultra-microtomes as well as a cryo ultra-microtome and a number of other interesting developments.
I worked for Fernandez-Moran at the U. of Chicago for a few years starting in 1963. This was after he was forced to leave Venezuela and then did a short stay at MIT before being recruited by U. of C. He had a microtome, presumably of his design, that only he used on rare occasion. Almost all the imaging done in the lab was with negatively stained samples.
He, of course, had his diamond knives. The technique to make them was perfected at the lab for neurological research that he built in Venezuela. (He was forced to leave Venezuela when the government was taken over by a military coup and he was on the wrong side, but that's another story.) He had a workshop there with his diamond cutters, etc. After developing the knives, he sent them out to the leading investigators of the day to get them to try them so that they would then buy them. Dupont picked up on the idea and also began making them for sale. Moran had patented the process so was able to sue Dupont and did win. I believe he later agreed to give them rights to make and market the knives.
Moran's lab at Chicago was quite a place. It was a semi-clean room lab in the basement of the Research Institute. The floors were raised so that all the water and vacuum lines for the microscopes were underneath with mechanical pumps and water recirculators a long ways away from the microscopes. He had 3 Seimens 1 and 1A TEMS on vibration mounts with the raised floor cut around the microscope bases so that moving a chair would not affect the TEM stability. A motor generator located in the attic of the building provided stable power. I started out as a technician and we all wore white nylon lab coats, white rubber shoes that we washed weekly, and little white hats. Visitors suited up in lab coats with plastic bags over their shoes. Pre-pumps to evacuate the film cameras were located two floors up. We would put on our red goggles to retain our dark adaptation, put plastic bags over our plastic shoes, and shuffle up to get new film cameras...looked like Martians!
It was an interesting time. There was also a couple of Japanese scientists working on an early Hitachi microscope trying to set resolution records. They would literally disassemble the microscope after just a few tries to clean it and get ready for the next attempts. They were the only ones with the patience to deal with that microscope. Later Perk and Elmer Company came along and helped with design changes to make it more user-friendly and thus more marketable.
Pointed filaments were also made in the lab. E.F. Fullam came one time to see how they were made and then was able to start selling them commercially.
Moran built a helium-cooled microscope to improve resolution using superconducting lenses. I do not know if it was originally his idea or if he "borrowed" someone else's idea. However, they built a liquid helium recirculator system, again with most of it in the attic 4 stories up. The helium flowed through a special jacket on the microscope that encased the entire upper part of the column through the objective lens. They managed to get a few images but then Moran lost interest. A series of health problems shortly thereafter led to closing down the lab and and end to a very interesting few years. Louie Ouwerkerk was a Dutch engineer who, along with the great U. of Chicago Instrument shop, designed and built a lot of Moran's ideas.
Debby
P.S. I have time to write this as the university is closed due to a major snow storm that hit us over the last 24 hours. Looks very pretty out there but....
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
On 2/2/11 6:43 AM, "terry.cooper-at-btinternet.com" {terry.cooper-at-btinternet.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers } } Sorry for the delay in response to this topic, but due to an error on my on } my part my previous communication went astray. For those who may be } interested in the history of ultramicrotomy I do have a copy of an article } in PDF form which traces the development of the ultramicrotome from "wedge" } sections in the late thirties where the thin end of the wedge was } (hopefully) transparent to electrons, through the high speed era (actually } up to 57000 rpm), overcoming embedding limitations and finally discussing } the first generation of commercial instruments. } } Please contact me on terry.cooper-at-btinternet.com and I can attach the } missive, } } Best regards } } Terry Cooper } TAAB Laboratories Equipment } 3 Minerva House, Calleva Park } Aldermaston, Berks, RG7 8NA, England } Tel ++44(0)118 981 7775 } Fax ++44(0)118 981 7881 } e-mail sales-at-taab.co.uk } www.taab.co.uk } } } ==============================Original Headers============================== } 6, 39 -- From terry.cooper-at-btinternet.com Wed Feb 2 05:39:05 2011 } 6, 39 -- Received: from nm5.bullet.mail.ird.yahoo.com } (nm5.bullet.mail.ird.yahoo.com [77.238.189.62]) } 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } p12Bd4GD004670 } 6, 39 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 05:39:04 -0600 } 6, 39 -- Received: from [77.238.189.57] by nm5.bullet.mail.ird.yahoo.com with } NNFMP; 02 Feb 2011 11:39:03 -0000 } 6, 39 -- Received: from [212.82.108.246] by tm10.bullet.mail.ird.yahoo.com } with NNFMP; 02 Feb 2011 11:39:03 -0000 } 6, 39 -- Received: from [127.0.0.1] by omp1011.mail.ird.yahoo.com with NNFMP; } 02 Feb 2011 11:39:03 -0000 } 6, 39 -- X-Yahoo-Newman-Id: 779398.69037.bm-at-omp1011.mail.ird.yahoo.com } 6, 39 -- Received: (qmail 86766 invoked from network); 2 Feb 2011 11:39:03 } -0000 } 6, 39 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 39 -- s=s1024; d=btinternet.com; } 6, 39 -- } h=DKIM-Signature:Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Mes } sage-ID:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encodi } ng:X-Priority:X-MSMail-Priority:X-Mailer:X-MimeOLE; } 6, 39 -- } b=IsObn+QLugwXVehYYiLw4s49xXwDqDZnhG9NNU4bIdzkofRt1VP0unu2tL6xZl2Sn4B0P6skvnc4 } oU3/Vz+MSfrTvXJJP0vwthYxKuegao2YJYJKxBY31StZ6EetQNgRNKsM9i+2WRIGannNqe0McTHQMO } CZ8dn6/wwzQ7mHXrA= ; } 6, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=btinternet.com; s=s1024; t=1296646743; } bh=oWdodXDAladTRTeAY1E2NNtKCYK+97yXwv8tXphMquI=; } h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:From:To } :Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:X } -MSMail-Priority:X-Mailer:X-MimeOLE; } b=VKuj8pFNykoHwXnX54sk3fQwJkOZuoFXszGFPDSrEBKHHYq1DhiRlqIpTJ0MbhCZ90mrkMGJGx7j } vganojRhWDRuoc0kwwPzAnbIlk/bme1OYZj+omRhbm7WR6+jbygWxaJiNoCrMyI3euTEnUXnS9XDuE } ToKBTrHyxyDEMCDBc= } 6, 39 -- Received: from Pioneer (terry.cooper-at-94.30.34.8 with login) } 6, 39 -- by smtp817.mail.ird.yahoo.com with SMTP; 02 Feb 2011 11:39:00 } +0000 GMT } 6, 39 -- X-Yahoo-SMTP: } pVuM3eCswBBmTWesC4KmNptnh5To2FPTm29gm3HoxqLR7BH.yfiY1w-- } 6, 39 -- X-YMail-OSG: NozSGW4VM1m6bRR8Zaj1i1Ns2oY8lipJOBHfz_emqEZD7Mz } 6, 39 -- zVSXR5KJ0BzFhcVKMQO.WbUOmXArrNSCPPZWh8tM6axZRAE73cHQ.ln61SO9 } 6, 39 -- DQkj6etKzZY_ajLLLC.zUoMqYjs2wMLSAAl2QujHC5Kb13GH4ItZm4d3Q64m } 6, 39 -- J6zDCnwQouOZrxCbBg4v2t3bwm5d9oDgemTErzM7csvNyzX8QVdqPncU6CZ8 } 6, 39 -- qx4oeuCZHTwoR4VAvY0LcYBzAhjEUfvcns1bVlRfFM_eK5_EWeFSz6DHdKqE } 6, 39 -- lLKPz1rsDlBI- } 6, 39 -- X-Yahoo-Newman-Property: ymail-3 } 6, 39 -- Message-ID: {8F6E8C47CA0E40BB89DFF1D968EE4462-at-Pioneer} } 6, 39 -- From: "terry cooper" {terry.cooper-at-btinternet.com} } 6, 39 -- To: {microscopy-at-microscopy.com} } 6, 39 -- Subject: Ultramicrotome history } 6, 39 -- Date: Wed, 2 Feb 2011 11:39:22 -0000 } 6, 39 -- MIME-Version: 1.0 } 6, 39 -- Content-Type: text/plain; } 6, 39 -- format=flowed; } 6, 39 -- charset="iso-8859-1"; } 6, 39 -- reply-type=original } 6, 39 -- Content-Transfer-Encoding: 7bit } 6, 39 -- X-Priority: 3 } 6, 39 -- X-MSMail-Priority: Normal } 6, 39 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 } 6, 39 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 } ==============================End of - Headers==============================
==============================Original Headers============================== 17, 31 -- From dsherman-at-purdue.edu Wed Feb 2 10:37:11 2011 17, 31 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 17, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12GbBEo011139 17, 31 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 10:37:11 -0600 17, 31 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu [172.21.6.92]) 17, 31 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p12Gb70v030385; 17, 31 -- Wed, 2 Feb 2011 11:37:07 -0500 17, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.190]) by WPPEXHUB03H.purdue.lcl 17, 31 -- ([172.21.6.92]) with mapi; Wed, 2 Feb 2011 11:37:07 -0500 17, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 17, 31 -- To: "terry.cooper-at-btinternet.com" {terry.cooper-at-btinternet.com} , 17, 31 -- "message to: 17, 31 -- MSA list" {microscopy-at-microscopy.com} 17, 31 -- Date: Wed, 2 Feb 2011 11:37:05 -0500 17, 31 -- Subject: Re: [Microscopy] Ultramicrotome history 17, 31 -- Thread-Topic: [Microscopy] Ultramicrotome history 17, 31 -- Thread-Index: AcvCzlqhWM0c9QOSSAKdkNfd5A5brwAKRJGZ 17, 31 -- Message-ID: {C96EF261.B8FB%dsherman-at-purdue.edu} 17, 31 -- In-Reply-To: {201102021143.p12Bh3sW007617-at-ns.microscopy.com} 17, 31 -- Accept-Language: en-US 17, 31 -- Content-Language: en-US 17, 31 -- X-MS-Has-Attach: 17, 31 -- X-MS-TNEF-Correlator: 17, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 17, 31 -- acceptlanguage: en-US 17, 31 -- Content-Type: text/plain; charset="us-ascii" 17, 31 -- MIME-Version: 1.0 17, 31 -- X-PMX-Version: 5.5.9.388399 17, 31 -- X-PerlMx-Virus-Scanned: Yes 17, 31 -- Content-Transfer-Encoding: 8bit 17, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p12GbBEo011139 ==============================End of - Headers==============================
We recently acquired a vintage Reichert- Jung, Cryocut 1800. Unfortunately, it is missing much of the knife holder assemble. Does anyone on the list have these parts and are willing to sell or surplus them? The unit is in good condition and should serve us well.
Thanks so much.
Steve
-- Steven Samuelsson, PhD Director Cell and Molecular Imaging Facility 100-51 SRI International, Inc. 333 Ravenswood Ave Menlo Park, CA 94061 (650) 859-2980
==============================Original Headers============================== 6, 18 -- From steven.samuelsson-at-sri.com Wed Feb 2 11:16:37 2011 6, 18 -- Received: from mail1.sri.com (mail1.SRI.COM [128.18.30.17]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12HGbQq011642 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 11:16:37 -0600 6, 18 -- MIME-version: 1.0 6, 18 -- Content-transfer-encoding: 7BIT 6, 18 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 6, 18 -- Received: from Steves-iMac.local ([unknown] [128.18.51.101]) 6, 18 -- by mail.sri.com (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built 6, 18 -- Jul 30 2009)) with ESMTP id {0LG0000CN2NOLRA0-at-mail.sri.com} for 6, 18 -- Microscopy-at-microscopy.com; Wed, 02 Feb 2011 09:16:36 -0800 (PST) 6, 18 -- Message-id: {4D499174.10708-at-sri.com} 6, 18 -- Date: Wed, 02 Feb 2011 09:16:36 -0800 6, 18 -- From: Steven Samuelsson {steven.samuelsson-at-sri.com} 6, 18 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.2.13) 6, 18 -- Gecko/20101207 Thunderbird/3.1.7 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: Cryostat Knife Holder Needed ==============================End of - Headers==============================
Great Story Debby, Thanks for sharing it with the list.
Stay Warm!
Best,
Neeraj.
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Wednesday, February 02, 2011 11:46 AM To: Neeraj Gohad
Fascinating article. Some of my recollections (possibly with some inaccuracies but the best I can recollect) involve Fernandez-Moran who is credited with developing the first diamond knives for use with ultra-microtomes as well as a cryo ultra-microtome and a number of other interesting developments.
I worked for Fernandez-Moran at the U. of Chicago for a few years starting in 1963. This was after he was forced to leave Venezuela and then did a short stay at MIT before being recruited by U. of C. He had a microtome, presumably of his design, that only he used on rare occasion. Almost all the imaging done in the lab was with negatively stained samples.
He, of course, had his diamond knives. The technique to make them was perfected at the lab for neurological research that he built in Venezuela. (He was forced to leave Venezuela when the government was taken over by a military coup and he was on the wrong side, but that's another story.) He had a workshop there with his diamond cutters, etc. After developing the knives, he sent them out to the leading investigators of the day to get them to try them so that they would then buy them. Dupont picked up on the idea and also began making them for sale. Moran had patented the process so was able to sue Dupont and did win. I believe he later agreed to give them rights to make and market the knives.
Moran's lab at Chicago was quite a place. It was a semi-clean room lab in the basement of the Research Institute. The floors were raised so that all the water and vacuum lines for the microscopes were underneath with mechanical pumps and water recirculators a long ways away from the microscopes. He had 3 Seimens 1 and 1A TEMS on vibration mounts with the raised floor cut around the microscope bases so that moving a chair would not affect the TEM stability. A motor generator located in the attic of the building provided stable power. I started out as a technician and we all wore white nylon lab coats, white rubber shoes that we washed weekly, and little white hats. Visitors suited up in lab coats with plastic bags over their shoes. Pre-pumps to evacuate the film cameras were located two floors up. We would put on our red goggles to retain our dark adaptation, put plastic bags over our plastic shoes, and shuffle up to get new film cameras...looked like Martians!
It was an interesting time. There was also a couple of Japanese scientists working on an early Hitachi microscope trying to set resolution records. They would literally disassemble the microscope after just a few tries to clean it and get ready for the next attempts. They were the only ones with the patience to deal with that microscope. Later Perk and Elmer Company came along and helped with design changes to make it more user-friendly and thus more marketable.
Pointed filaments were also made in the lab. E.F. Fullam came one time to see how they were made and then was able to start selling them commercially.
Moran built a helium-cooled microscope to improve resolution using superconducting lenses. I do not know if it was originally his idea or if he "borrowed" someone else's idea. However, they built a liquid helium recirculator system, again with most of it in the attic 4 stories up. The helium flowed through a special jacket on the microscope that encased the entire upper part of the column through the objective lens. They managed to get a few images but then Moran lost interest. A series of health problems shortly thereafter led to closing down the lab and and end to a very interesting few years. Louie Ouwerkerk was a Dutch engineer who, along with the great U. of Chicago Instrument shop, designed and built a lot of Moran's ideas.
Debby
P.S. I have time to write this as the university is closed due to a major snow storm that hit us over the last 24 hours. Looks very pretty out there but....
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
On 2/2/11 6:43 AM, "terry.cooper-at-btinternet.com" {terry.cooper-at-btinternet.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers } } Sorry for the delay in response to this topic, but due to an error on my on } my part my previous communication went astray. For those who may be } interested in the history of ultramicrotomy I do have a copy of an article } in PDF form which traces the development of the ultramicrotome from "wedge" } sections in the late thirties where the thin end of the wedge was } (hopefully) transparent to electrons, through the high speed era (actually } up to 57000 rpm), overcoming embedding limitations and finally discussing } the first generation of commercial instruments. } } Please contact me on terry.cooper-at-btinternet.com and I can attach the } missive, } } Best regards } } Terry Cooper } TAAB Laboratories Equipment } 3 Minerva House, Calleva Park } Aldermaston, Berks, RG7 8NA, England } Tel ++44(0)118 981 7775 } Fax ++44(0)118 981 7881 } e-mail sales-at-taab.co.uk } www.taab.co.uk } } } ==============================Original Headers============================== } 6, 39 -- From terry.cooper-at-btinternet.com Wed Feb 2 05:39:05 2011 } 6, 39 -- Received: from nm5.bullet.mail.ird.yahoo.com } (nm5.bullet.mail.ird.yahoo.com [77.238.189.62]) } 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } p12Bd4GD004670 } 6, 39 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 05:39:04 -0600 } 6, 39 -- Received: from [77.238.189.57] by nm5.bullet.mail.ird.yahoo.com with } NNFMP; 02 Feb 2011 11:39:03 -0000 } 6, 39 -- Received: from [212.82.108.246] by tm10.bullet.mail.ird.yahoo.com } with NNFMP; 02 Feb 2011 11:39:03 -0000 } 6, 39 -- Received: from [127.0.0.1] by omp1011.mail.ird.yahoo.com with NNFMP; } 02 Feb 2011 11:39:03 -0000 } 6, 39 -- X-Yahoo-Newman-Id: 779398.69037.bm-at-omp1011.mail.ird.yahoo.com } 6, 39 -- Received: (qmail 86766 invoked from network); 2 Feb 2011 11:39:03 } -0000 } 6, 39 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 39 -- s=s1024; d=btinternet.com; } 6, 39 -- } h=DKIM-Signature:Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Mes } sage-ID:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encodi } ng:X-Priority:X-MSMail-Priority:X-Mailer:X-MimeOLE; } 6, 39 -- } b=IsObn+QLugwXVehYYiLw4s49xXwDqDZnhG9NNU4bIdzkofRt1VP0unu2tL6xZl2Sn4B0P6skvnc4 } oU3/Vz+MSfrTvXJJP0vwthYxKuegao2YJYJKxBY31StZ6EetQNgRNKsM9i+2WRIGannNqe0McTHQMO } CZ8dn6/wwzQ7mHXrA= ; } 6, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=btinternet.com; s=s1024; t=1296646743; } bh=oWdodXDAladTRTeAY1E2NNtKCYK+97yXwv8tXphMquI=; } h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:From:To } :Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:X } -MSMail-Priority:X-Mailer:X-MimeOLE; } b=VKuj8pFNykoHwXnX54sk3fQwJkOZuoFXszGFPDSrEBKHHYq1DhiRlqIpTJ0MbhCZ90mrkMGJGx7j } vganojRhWDRuoc0kwwPzAnbIlk/bme1OYZj+omRhbm7WR6+jbygWxaJiNoCrMyI3euTEnUXnS9XDuE } ToKBTrHyxyDEMCDBc= } 6, 39 -- Received: from Pioneer (terry.cooper-at-94.30.34.8 with login) } 6, 39 -- by smtp817.mail.ird.yahoo.com with SMTP; 02 Feb 2011 11:39:00 } +0000 GMT } 6, 39 -- X-Yahoo-SMTP: } pVuM3eCsw